LOCUS       DAZKLA010000001        61088 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000001 DAZKLA010000000
VERSION     DAZKLA010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.001"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(31..1422)
                     /gene="sufB"
                     /locus_tag="ACG85N_000001"
     CDS             complement(31..1422)
                     /gene="sufB"
                     /locus_tag="ACG85N_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFQ0222813.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(1427..1897)
                     /gene="sufU"
                     /locus_tag="ACG85N_000002"
     CDS             complement(1427..1897)
                     /gene="sufU"
                     /locus_tag="ACG85N_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFQ0222814.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(1884..3119)
                     /locus_tag="ACG85N_000003"
     CDS             complement(1884..3119)
                     /locus_tag="ACG85N_000003"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFQ0222815.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(3116..4402)
                     /gene="sufD"
                     /locus_tag="ACG85N_000004"
     CDS             complement(3116..4402)
                     /gene="sufD"
                     /locus_tag="ACG85N_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFQ0222816.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(4415..5188)
                     /gene="sufC"
                     /locus_tag="ACG85N_000005"
     CDS             complement(4415..5188)
                     /gene="sufC"
                     /locus_tag="ACG85N_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFQ0222817.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(5385..6233)
                     /locus_tag="ACG85N_000006"
     CDS             complement(5385..6233)
                     /locus_tag="ACG85N_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0222818.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(6257..6943)
                     /locus_tag="ACG85N_000007"
     CDS             complement(6257..6943)
                     /locus_tag="ACG85N_000007"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0222819.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(6936..7970)
                     /locus_tag="ACG85N_000008"
     CDS             complement(6936..7970)
                     /locus_tag="ACG85N_000008"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0222820.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(8033..8289)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8365..8703)
                     /locus_tag="ACG85N_000009"
     CDS             complement(8365..8703)
                     /locus_tag="ACG85N_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFQ0222821.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(8705..9061)
                     /locus_tag="ACG85N_000010"
     CDS             complement(8705..9061)
                     /locus_tag="ACG85N_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFQ0222822.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(9067..10254)
                     /locus_tag="ACG85N_000011"
     CDS             complement(9067..10254)
                     /locus_tag="ACG85N_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0222823.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(10445..11290)
                     /locus_tag="ACG85N_000012"
     CDS             complement(10445..11290)
                     /locus_tag="ACG85N_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0222824.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            11524..12489
                     /locus_tag="ACG85N_000013"
     CDS             11524..12489
                     /locus_tag="ACG85N_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFQ0222825.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            12866..13909
                     /locus_tag="ACG85N_000014"
     CDS             12866..13909
                     /locus_tag="ACG85N_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFQ0222826.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(13971..14435)
                     /locus_tag="ACG85N_000015"
     CDS             complement(13971..14435)
                     /locus_tag="ACG85N_000015"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFQ0222827.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            14681..15628
                     /locus_tag="ACG85N_000016"
     CDS             14681..15628
                     /locus_tag="ACG85N_000016"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0222828.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(15839..17911)
                     /locus_tag="ACG85N_000017"
     CDS             complement(15839..17911)
                     /locus_tag="ACG85N_000017"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0222829.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(17844..18221)
                     /locus_tag="ACG85N_000018"
     CDS             complement(17844..18221)
                     /locus_tag="ACG85N_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFQ0222830.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(18350..18910)
                     /locus_tag="ACG85N_000019"
     CDS             complement(18350..18910)
                     /locus_tag="ACG85N_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0222831.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            19060..20352
                     /locus_tag="ACG85N_000020"
     CDS             19060..20352
                     /locus_tag="ACG85N_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0222832.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(20412..21530)
                     /locus_tag="ACG85N_000021"
     CDS             complement(20412..21530)
                     /locus_tag="ACG85N_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFQ0222833.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(21544..22965)
                     /gene="arcD"
                     /locus_tag="ACG85N_000022"
     CDS             complement(21544..22965)
                     /gene="arcD"
                     /locus_tag="ACG85N_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFQ0222834.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(23211..24332)
                     /gene="mnmA"
                     /locus_tag="ACG85N_000023"
     CDS             complement(23211..24332)
                     /gene="mnmA"
                     /locus_tag="ACG85N_000023"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFQ0222835.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            24736..25335
                     /locus_tag="ACG85N_000024"
     CDS             24736..25335
                     /locus_tag="ACG85N_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0222836.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(25473..26759)
                     /gene="efmA"
                     /locus_tag="ACG85N_000025"
     CDS             complement(25473..26759)
                     /gene="efmA"
                     /locus_tag="ACG85N_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFQ0222837.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(27030..27374)
                     /locus_tag="ACG85N_000026"
     CDS             complement(27030..27374)
                     /locus_tag="ACG85N_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFQ0222838.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(27511..28656)
                     /locus_tag="ACG85N_000027"
     CDS             complement(27511..28656)
                     /locus_tag="ACG85N_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0222839.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(28836..29810)
                     /locus_tag="ACG85N_000028"
     CDS             complement(28836..29810)
                     /locus_tag="ACG85N_000028"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0222840.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(29890..30765)
                     /locus_tag="ACG85N_000029"
     CDS             complement(29890..30765)
                     /locus_tag="ACG85N_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFQ0222841.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            30895..32802
                     /locus_tag="ACG85N_000030"
     CDS             30895..32802
                     /locus_tag="ACG85N_000030"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFQ0222842.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(33343..36621)
                     /locus_tag="ACG85N_000031"
     CDS             complement(33343..36621)
                     /locus_tag="ACG85N_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFQ0222843.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(36892..37437)
                     /locus_tag="ACG85N_000032"
     CDS             complement(36892..37437)
                     /locus_tag="ACG85N_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0222844.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(37449..38141)
                     /locus_tag="ACG85N_000033"
     CDS             complement(37449..38141)
                     /locus_tag="ACG85N_000033"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFQ0222845.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(38160..38444)
                     /gene="macP"
                     /locus_tag="ACG85N_000034"
     CDS             complement(38160..38444)
                     /gene="macP"
                     /locus_tag="ACG85N_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFQ0222846.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(38457..39020)
                     /locus_tag="ACG85N_000035"
     CDS             complement(38457..39020)
                     /locus_tag="ACG85N_000035"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0222847.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            39248..39910
                     /locus_tag="ACG85N_000036"
     CDS             39248..39910
                     /locus_tag="ACG85N_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFQ0222848.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            40034..41230
                     /locus_tag="ACG85N_000037"
     CDS             40034..41230
                     /locus_tag="ACG85N_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFQ0222849.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(41331..42707)
                     /locus_tag="ACG85N_000038"
     CDS             complement(41331..42707)
                     /locus_tag="ACG85N_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0222850.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(42744..43610)
                     /locus_tag="ACG85N_000039"
     CDS             complement(42744..43610)
                     /locus_tag="ACG85N_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0222851.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(43699..44724)
                     /locus_tag="ACG85N_000040"
     CDS             complement(43699..44724)
                     /locus_tag="ACG85N_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0222852.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(44742..45416)
                     /locus_tag="ACG85N_000041"
     CDS             complement(44742..45416)
                     /locus_tag="ACG85N_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0222853.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(45600..46493)
                     /locus_tag="ACG85N_000042"
     CDS             complement(45600..46493)
                     /locus_tag="ACG85N_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0222854.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(46567..48264)
                     /locus_tag="ACG85N_000043"
     CDS             complement(46567..48264)
                     /locus_tag="ACG85N_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0222855.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(48257..49570)
                     /locus_tag="ACG85N_000044"
     CDS             complement(48257..49570)
                     /locus_tag="ACG85N_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0222856.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(49570..53511)
                     /locus_tag="ACG85N_000045"
     CDS             complement(49570..53511)
                     /locus_tag="ACG85N_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0222857.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(53620..54078)
                     /locus_tag="ACG85N_000046"
     CDS             complement(53620..54078)
                     /locus_tag="ACG85N_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0222858.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(54376..54894)
                     /locus_tag="ACG85N_000047"
     CDS             complement(54376..54894)
                     /locus_tag="ACG85N_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0222859.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(54896..55512)
                     /locus_tag="ACG85N_000048"
                     /pseudo
     CDS             complement(54896..55512)
                     /locus_tag="ACG85N_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            complement(55509..56297)
                     /locus_tag="ACG85N_000049"
     CDS             complement(55509..56297)
                     /locus_tag="ACG85N_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0222860.1"
                     /translation="MTVIGIILIIPLLWMVFTSLKPMEEIVRYPPTFFPEKIVWENYL
                     DTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYGFAKLDFPGKKLMFALVLSTMMI
                     PGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFNIFLMRQFYLSINNELIEAAEID
                     GANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFLGPLLYIQDQEKYTLQIGLQVFQ
                     NQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGMDLTGGSKG"
     gene            complement(56359..57270)
                     /locus_tag="ACG85N_000050"
     CDS             complement(56359..57270)
                     /locus_tag="ACG85N_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0222861.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(57311..58645)
                     /locus_tag="ACG85N_000051"
     CDS             complement(57311..58645)
                     /locus_tag="ACG85N_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0222862.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            58853..59683
                     /locus_tag="ACG85N_000052"
     CDS             58853..59683
                     /locus_tag="ACG85N_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFQ0222863.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(59927..60176)
                     /locus_tag="ACG85N_000053"
                     /pseudo
     CDS             complement(59927..60176)
                     /locus_tag="ACG85N_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(60367..60561)
                     /locus_tag="ACG85N_000054"
     CDS             complement(60367..60561)
                     /locus_tag="ACG85N_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0222864.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT        17240 a        13151 c        11000 g        19697 t
ORIGIN      
        1 atatcaaggg ttctagctcc tataacaggt ttacccaaca ctgccttcca tctcataact
       61 gatcaatcga ttgagttcga ctgcatattc cattggaagt tctttggtga atggttcaac
      121 aaagcccatg acgatcattt cagttgcttc agattcggtc aggcctcggc tcatcagata
      181 gtagagttgt tcttctgaga tttttgagac tttggcttcg tgttctaaag agacttggct
      241 gttgtggatc tcattgaatg ggatcgtatc ggatttcgac cattcgtcca tgatgatcgt
      301 gtcacattcg atatgagaga tcgaacccgc actgtttttg gcaaaggtta cttgtcctcg
      361 atagtttact tctccgccgt cttttgaaat tgatttagag acgatagagc tagatgtatt
      421 tggtgcattg tggatcattt ttgcacccgt atcttggatt tggtttgctc ctgcaaaggc
      481 gatggagagc atggtgcctc gtgcaccttt tccatctaag taaacacttg ggtatttcat
      541 cgttgttttt gcacctaggt ttccgtcgat ccattcaacg gttgcgcctt cgtatgcttt
      601 tgctcgtttg gttactaagt tgtacacatt gtctgaccag ttttggatcg ttgtgtaacg
      661 gcaatacgca tcttttcttg taaagatttc tacgatggcg gcatgcaggc tgttacttga
      721 ataagtaggt gccgtacaac cttcgacata gtggatgctt gctccttcgt caacaatgat
      781 cagcgtacgt tcgaattgcc ccgtgttttc cgcattgatc cggaagtacg tttgtaatgg
      841 aacatcgact ttgacgcctt ttggcacgta aataaaggtc ccacctgacc atacggctga
      901 attcagcgct gctaatttgt tgtcggttgg cggtacaagt tttgaaaagt actctttgaa
      961 taattcaggg tattctttca aagcagagtc agtgtcagta aagacgattc ctaacttttc
     1021 gaattcatct ttcatattat ggtacacgac ttctgattca tattgtgcgg aagcaccggc
     1081 tagataagct cgttcagctt ctggtatacc aatcttttca aatgtatctt tgattttttc
     1141 tggtacgtct tcccagtcac gagcgggttt gtcacttggt ttttggtaat atttgatggc
     1201 atcgaaatcg atgtctgaaa ggtcggggcc ccatgtttgc attggcattt tgttaaatgt
     1261 ttctagagat ttcagacgga attcaagcat ccattctggt tcgcctttga ttcttgacat
     1321 ctcacggatc acttcttctg tcaagccttc tcctgtactg aaaaccggtt cgacatcatc
     1381 gtgaaacccg aatttatatt cttctaattc tggtacgccc atgttctcac tcctcttcct
     1441 tgtggtaatg atggaaagct gcggtaccat cgttttccat tgcttttcct agtgctttcc
     1501 aggcaagtgt cgcacatttg atccgagcgg gaaatttagc tacaccacca agcatagcag
     1561 catcgcctaa ttggtcggga tctttgactt cttttccttg gacaagctgt agaaagtcta
     1621 cagttaaatg ttctgcttct tctattgatt ttccgatgac ggcgtctgtc atcatcgagg
     1681 cacttgccgt actgatggag cagccactgc cgtcaaaggc gatatttttg atgattccgt
     1741 cttttacatc gacttgcaat tcgatcacat ctccacatgt ggggttgttc aattcgatct
     1801 tgcttgtcga gtcagtcaag cttccacgat gatgcggatg ggaagaatga tccaagatca
     1861 cttgacggta taaattgtct aatttagata gtcccatgtt ggaaaaactc ctttgttgcc
     1921 atgattgctt caactaaacg gtcggcgtct tgcgttgtgt tgtagaaata aaagctcgcg
     1981 cgagcagtcg ctgatacttc aaggtacttc aatagcggtt gcgcacaatg atgacctgct
     2041 ctgacggcta ccccttccat gtctaaagct gtcgcgacat catggggatg taacccatcc
     2101 aaattgaatg caacgactcc tgtccgaaaa ttcgggtctt gtggaccata aacagtcaat
     2161 ccatcaattg cttgtaattt tggcaacaca taagcggcta gttcttcttc atatcgatgg
     2221 atattgtcca taccgagttt ttgcaaataa tcgattgcat ggcctaatgc gatagcgccg
     2281 gcgatatttg gtgtccctgc ttcgaatttc caaggtagtt ctttccatgt actgtcgtat
     2341 aattcgacaa aatcgatcat ttcgccacca aattcaacag gttccatttc ttctaaccat
     2401 tttcgtttac catataaaac accaatacct gttggtccgc acattttgtg tccgctgaaa
     2461 gcaaagaaat cacagtctag tgcttggaca tcaatggcca tgtgcggggc agattgagca
     2521 ccatctacta cgatgacccc gccgttttca tggacaagtt cagctaattc ttgaacagga
     2581 ttgaagacac ctaagacatt ggatacatga gcaatggaga cgattttggt tttgtccgta
     2641 atctttttcg ctgcatcctc catatccagg aagccttctt ctgttagttc gatatatttc
     2701 aagacagcac ctgttcgttt tgccaactgc tgccacggga taacattaga atgatgttcc
     2761 atgtaagaga gcactatctc gtctcctgct ttgatatagc ggtcgccgaa ggttctggct
     2821 acccaattca agcttgtcgt cgtaccacga gtaaacagca cttctgctgt ttcttttgca
     2881 tggatgaacg cccgtacttt ttctcttgct tgttcataat catgggtcgc gcgttctgct
     2941 aacgtatgga caccgcgatg aacattggcg ttatcgtgtt cgtaatagta cgctaatgcc
     3001 tggagtactt gttttggctt ttgggtcgtc gctgcattat ccagataaac taaaggttca
     3061 tcattgacgt cttgaaataa gataggaaaa tcattgcgta gctttttagc atcgatcatc
     3121 cattcaactt cccttcgatt acttcgacta attctttccg aacttgttct acagggattg
     3181 ctgttaagac agaaccgagg aatcctcgaa tcactaagcg ctctgcttct tctttatgca
     3241 atcctctact catcagatag tacatttctt ctggatcaac tcgtcctaca ctggcagcat
     3301 gtccagcagt tacttcgttt tcatcgatca aaagaatagg attcgcatca ccacgtgctt
     3361 tatcagaaag catcaagaca cgactttctt gttgggcatc tgcacctttt gcccctttca
     3421 agatgtggcc gataccatta aaggtcaatg ttcctttttc tcggatcact ccgtgttgca
     3481 aaatatgccc gatcgtatga ggggctttat tggttactcg tgtatcgatt ccttggattt
     3541 gtctgcccga gctgattgcc acgattttga cttctgcgtg tgcaccttct cctgctaaat
     3601 ctgagtcaaa gtcagcgatc acatgaccgt cattcatgac accaattgcc cagtcgacag
     3661 aagcatctcg taaaatgtgc ccacggcgat tcatataaga agtaatattt tctcctaatt
     3721 gatctacggc tgaatatttg atttttgaac cagctttagc gatgacttcc acgatgatat
     3781 tacctgatga ttttgctact tgttctttgg ttgtctggaa acgttccaga taagaaaatt
     3841 cactatgttc atctgccacg atcaacacat gcttgaagaa gtgctcatcg gatgcgccat
     3901 cttgaatgaa taaggactcg agtggttcat cgatcacgac atttttaggc acatacagga
     3961 aaataccgct gttcataaaa gctacatgga gtgcagtcaa ttggtcttcg ttcatgttta
     4021 ctgctttttt catataatat tcttggacta gttctggata ttcgatcatt gcagtgaaca
     4081 agtcggtaaa aatgactcct ttttctgcta attccacagg cagttgttca aaaatcgtcc
     4141 atgaaccttg ttggacaagc acaggattgt ctttcattgc atcaaatgcc ggaatcgtgc
     4201 ctgtttcact gatggtttgt gtttctttca catcaaataa cgaccagcga tggaatttca
     4261 cccgatcgat atgcggcaat ggcagttcat ctgctttcgc tagtgcgtct tgacggaagg
     4321 ttctcatcca atcaggttcg cctttactta aagaaaaatc atttaccgcg tcaagataat
     4381 cgagccagtt tttttctttc attcttgctc ccccttacgc ttcttcttct ttatagtcga
     4441 ttcctaattc tttactgata ccagcgtagc cttctgcttc cagacgttta gctaggtcag
     4501 cattccctgt catgactact cggccttcca tcatgatgtg aacgacatca ggtgtgatat
     4561 agttcaataa tcgttgatag tgggtgatga tcaatgcacc gaaattgtct ccgcgcattt
     4621 catttactcc tttagcaacg actttcaaag catcgatatc cagacctgaa tcgatttcgt
     4681 ctaaaattgc aaatgtaggt tctaacatca acaattgcag gatctcattc cgtttctttt
     4741 ctccgccaga aaaaccttcg ttcagataac gttcagccat ttcttctggc atatttagta
     4801 aagccatttt ttcatctaac tttttcaaaa atgccataac agaaattttg tcgttttcat
     4861 cacgtttcgc attgatcgct gcacgcatga attccgcatt cgtgatacct ggtatctcac
     4921 ttggatattg catagctaaa aataagccaa gtcgcgcacg ttcatctact tccaagtcta
     4981 aaacattttt cccgtcaaac aaaatttcgc cttctgttac ttcatagttt ggattgccca
     5041 tgatagcggc agataaggta gattttcctg tgccgttagg tcccatgatg gcatggattt
     5101 ctccggtttt cattgttagg tttacgcctt taaggatttc tttttcctcg atcgacacat
     5161 gtagattttt tatttctaag acagacatgc attatacccc caaattctct ttcgttctca
     5221 ctcattttag actaattgag aatgaagcgc aatgaccttc cttactaaaa tcaaatatct
     5281 tttatttatt attaaacaat tttgatttct aaattagaat aggtataaaa aagatgagaa
     5341 ataagccatt acagacttat ttctcatctt ttttgttcca taccttattt tgcatcagcc
     5401 tttactggtt taacagagcc gccccatttg tctgtgatcc attcttggac ttctttgcgg
     5461 tgaagcactt caatcagttt tttgattttg tcactgtctt catcaccttt acgagtcgca
     5521 ataatattca cgtaaggaga gttgtccttt tcaagcaata cggcatcttc taatggattc
     5581 aaccctgcgc cgtaagcgaa gtttgcgttg attgcaacta ggtcaccttc atcattttgg
     5641 tacgtagaag ctaaaagtga tgggtcgatc gtatggttga acttcaagtt cttagggttt
     5701 tcttcaatat catcgaatgt tgctgtttcc agatctactc catctttgac tttgactaag
     5761 ccagcatctt gtagaattgt gatgatgcgt ccccagtctg attcagaatt tgaagtaatg
     5821 attgttgcac catcttttaa ttcggaaaca tctttgactt ttttagagta caagcccatt
     5881 ggttcgatgt ggacagcacc ggcattgaca aagtcatatc ctttttcttt gatttctttg
     5941 ttcaaataag gaatatgctg gaagtaattg gcatcaatgt cgccttcttc caaagattgg
     6001 ttcggtaata catagtcatc gaatttgacg atttcaagat cgattccttc atctttcaat
     6061 aatggtttca catgttccaa aatctctgcg tgcggtgtcg gtgaagcgcc gactttcaat
     6121 gtcgtgtcgt ctgctttatt tgctgaatct ttgctgtcgc tgttgttacc gcatgctgct
     6181 aatcctacag tcagcaaaag cgctgctgca aatccaaaaa ttttcttttt catatcctac
     6241 atccccctat atcctattat cttttgtctg ttttcttagt taagaaatca ccgattcctt
     6301 gaacgataaa tacgattagt aaaataatta aagtcgcaac gaatgtcact gttagattac
     6361 cacgctggta gccttcctgc caagcaagac ctccaagtcc acctgcgccg atcgcgccgg
     6421 ccatcgcagt aaatccgatc atcgagatcg ttgtaactgt taatccagaa atcaatgcgg
     6481 gaatactttc aggtatcaag accttccaga tgatttccca gtaacttgct cccattgcat
     6541 ctgctgcttc cagtacacca gggctgactt cacgaaaagc aatctctact agacgggcat
     6601 aaaatggtgc tgcggataaa acaagagctg gaatggctgc ttgcgcacct agcatagtgc
     6661 ctacgattgc tttagtaaat ggaatgatca aaaccatcag gatcataaat ggcgttgacc
     6721 ggaaaatatt actgatgatc gatacgattc cataaatgat tcgagcatac ggtttgtttt
     6781 tgcgtccaat agagtaaagg atcaatccca aaactaatcc gatgatcgtc actaagacca
     6841 ttgagattaa tgtcatatat agtgtatcaa ggatagcctg attcatcgtt tccgggttga
     6901 tttgtttaaa atctagatat gtttctgaaa agcttttatc cacgatggat cacctcaatt
     6961 cctactcctt gttctttgat gaacacttct gcttgatcta attctttggt ttcaccagtc
     7021 atcaagacag tcaatgaacc aaaggaacct tctttcactt gtttgatctt tccatacaca
     7081 acgttgacat cgatgttata gcgtcgaatc gcttgggaaa tgactggctc gttggcattt
     7141 ctttcgctaa aggtcaaagt tgccaaacgg ccgcttggat tttctttgat gagttcagct
     7201 aatagttctg tcgaatcagc ttctggttcg atgtcttgtt gtacaaagcg tttggttact
     7261 tcttttttcg gttgtctgaa gacagtcaat acgtctcctt cttctacgac tttccccaac
     7321 tccatcacag cgactttgtt gcagattttg cggatcacat tcatttcatg tgtgatcaag
     7381 acgatggtca aattcaattt cttattgatc gctaacaata agtctaatac ttcttctgtt
     7441 gtttgcgggt ccaatgcgct agtagcctca tcacaaagca ggatctcagg atcatttgcc
     7501 aatgctcgtg cgataccgac gcgttgtttc tgtccgccgg aaagttgtga aggatatgct
     7561 tgtccgcgtc cttctaaccc gactaaacgc aacagttctt ctgcccgttc tttacgtttg
     7621 ctctttggta cgccagctaa ttccagcggc agctggatat tttcaagtac agttcttgac
     7681 cataacaggt tgaaatgctg gaagatcata ccgattttct ttcggaatgt acgtaattct
     7741 ttgtttttta attttgtaat atcttgatcg tttacgatca cttcgccgct cgtgggaagc
     7801 tcaagtccat tcaggagacg gactaatgta ctttttcctg cgccagaata gccgacgatt
     7861 ccataaatat cccctttttc aacgtttagt gaaacgtcat tgacggcttg gatgtttccg
     7921 tttttcccat tgaatgtctt tttgacattc gttagttgga ttaatgacat gccattcaca
     7981 tctttctctt ataatttttc aaacaaaaaa gccttcgccc tcttgcctta agcaaaagga
     8041 cgaaagcttg aacttccgtg ttaccacctt gatttaccgt tacttcgcaa taacagcctc
     8101 ttccagtact tggacttagc ttctttccgt atactgtcgc gctgtaacgg gcactcccgt
     8161 agtaagtttg cttacgctcg cttactctgc tctaagacca tcttccatct cattttcgtt
     8221 acccattttc agcaaacagg gctctcttca aacgtccgtg acatgtactc ttcttttcaa
     8281 agctttctcg tttcatatag gattgcttat gattatagca actggaagaa tcagtgtcaa
     8341 cttttttcaa gtgattttct gattttataa ttcgtcaaat tcttttggat caacatcttt
     8401 gaaacttaag atccacgcat tcatttcatc tgaatcattc aagatattga tatcttcacc
     8461 gattttatca ttaacagaag aaactgtacc cgttagcggg ctggcaaatt cattgaccgc
     8521 tttttccgct tcaacttcga tcagaggttc atcttttttt agcgattgtc ctgcttttgg
     8581 cagattggca aacgtgatgt ctccaagttc ttcttgcgct tcatttgtca aaccaacaca
     8641 gtattctttt ccattaaata agatccaaag gttgtccttt ttcttcaaat gcttgcctgc
     8701 cattttatat cagttctcct tcataaattt tttcattgaa tccaattgca acaagctggt
     8761 cctctcgtaa gatcagtggt cgcttgatca gcataccatc tgtcgataag acttggctcg
     8821 cttttgaagc cgtaaactcg cctacttgat ctttcagtcc aagttcccga tatttcatcc
     8881 cgcttgtgtt gaacaggcga cggattggta cttgactcgt ttctagccaa gtctcaatcg
     8941 ttgaggcctt cggtacttct ttgatcatat caacggtttg ataactaacg ccatgctggt
     9001 ctaaccacgc tttggctttt ttgcatgttg aacatttcgg gtaccagtaa aaggtgtaca
     9061 attttcttac ctccttgaag atcttgcagg tgcttcattt tggtatctca tcgataatat
     9121 caacccgata ccgatcatgt tccctaaaat cgaggatcct ccctgactaa tgaaaggcaa
     9181 tgggatccct gttaaaggaa gtaatccgat atttgctcca atattctcaa agacatggaa
     9241 cagcagcatc atgatcaaac cgcttgcgat ataggcgtaa aattcattat tcgtatcgaa
     9301 acacacccga atcatacgat agattaaaat aaaatacaag aaaatgacga atgcaccacc
     9361 gataaagccg aagttttcac cgataacggt aaaaatcata tcggactcac gaacaggcac
     9421 atagatattg cttacgttga atcctttacc aaacaagcca ccggtaccaa ttgccaaaag
     9481 tccttgggcc ggctgataac tcattcctga cgtatcatgg aaagggtcca gccatgaatc
     9541 aatacgtgca aactgataag atttgaatcc aactttataa agcaagtctc ttccgccttc
     9601 agtcgtaacc aagaagatcg ttcctgctcc aacgactact gccaagatta ctacaggtgc
     9661 gatgatgcgc caagagattc cagacatcag gaaaatcccg ccaaagatcg caagaaagac
     9721 aagcatcgtt ccaaagtcat cttgggccat gatcaatgct aaaaccggta ttgtcacgat
     9781 caagagcttc ccaatcagcc aaaagtccga tttcatcgtc cgttctttta cttgcgtatt
     9841 atgtttagtt acgatcagtg ccatcatcaa gatgtacgca atcttcatca actctgctgg
     9901 ctggaaagta aacgcaccga atcgaaacca gtttttagac cctgttgaag cagctaaatt
     9961 ctgatcatag aatttgagca agagtcccat taccactaac cccaaaccat aaaggtaagg
    10021 agtcagtttc cagatccatt tggaattcaa atgcatgatg acaacgattg ctgccgctcc
    10081 aactaaatac cagacaactt gcatccctac gcctttcaag actgctggat tgttcggatc
    10141 atggctaagt gctacatata atgagagcag accaatcaaa cataacagaa agactggtaa
    10201 aatcacgcca taatcaatac gattatcgtt cgaagctttt aatttttttt ccatgtttca
    10261 tcccttttct taaaaaccat actgctgatt attatagcgg tccagttaga taatggaaag
    10321 tctttccata gacatttggg aaaaattaac tttccgtttg tgcagttgct tgagcaaatg
    10381 cagtacaaaa gcctactgct tatctttagt gtatcataga actttttttg atacatgcaa
    10441 ttatctacca gctgagattc tcctcgctgt gagaagtttc tgtgctttca ttgtatcgaa
    10501 ggatctcttc ggcaatctct agcagctgat cagaaaccag attggttcgt tggatgaaat
    10561 aaaaatatcg atggaaattc aacggagtca ttggcacatt ttctggaaca gagcgcttag
    10621 acaaaatcga gcgaccgatt ccttccttca gcaacgcaac aattacatcg ttacttttca
    10681 cgatcatctt cttatcttgg atattcatct cttcaaaata acgctgcgtg taatgaaaaa
    10741 caccagagtt tgcttctctt acaagccaca atttgctttt tgggtcccca gcgatgacta
    10801 gctggtcttc catcaaagag tatctttgta cgccggaagt ctctaatggc ttttcgataa
    10861 aaccaaaatc ggattcatgt ttcgctactt gttctaatga ttcatgagaa ttcgtgattt
    10921 cgatctcaaa atgcacttta ggaaaccttt tgatcagatg tttcatcaac tgaggcaaaa
    10981 gatagacagc aaaggtgtgg gaagcaacga tccgacatac ttcttcttca ttttgttcat
    11041 tgagcaattt ttgctgagtt tcttcccatt cttctaacaa aaccagtgtc cgttcatata
    11101 ataagtcggc ttgcttagtt ggaagcatct cttttcttcc gttacgttga aaaagatcga
    11161 tattcaattc ccgctccaac tgtttgattt gattcgagac tgctggttga gataaaaaca
    11221 gtacttctgc tgctctggaa aaatttcttg tttcatatac tgctcgaaaa gtcgttaata
    11281 acttaaacat ggcaccactt ccataacttt ttatgataat tactataaaa actatttgat
    11341 ttaaatatac aaaaaaccat ctataataga aatgtacaac aaaacagttg ctgatccaac
    11401 tagtaactta gatgaagaca atcagctgtc tgcttgctcg taatcataca ttcctattca
    11461 ttttacaatg tattcgttca tcttggtact aaaatctatt aaataaaaag gagaatatta
    11521 tatatgaaga actttttgaa aattattccg atcccgatct gcgggttgat ccttgggtta
    11581 gcttcccttg gaaatctgtt taaagactat caccatgttg ctttaggcaa tctaactggc
    11641 ggtatctcta tgattcttat gatattcatt tttgttaaat tgtttatctt attcgaacac
    11701 acgaaacaaa cgttgcatga tccaatcatc gcctccgttt caccaacttt tacgatgtca
    11761 ctcatggtta tttgtactta ttttgtttca tatgaaagta ttgcaccaat tgtcaaattc
    11821 atttggctgt gcgctgttat cttccaagtg atccttgtct tttatttcaa ttatcaccat
    11881 gttatcaaag ctgatctttc aattgaagca atttatccaa gctggtttat catttacgtt
    11941 ggatttgggg tcatcactgt aactgccggg aacttcttcc cacttattgg aaaaattttc
    12001 ttttggattt cattgatctg ctatgcgttc ttattgccga tcatcatcaa acgtgtcttt
    12061 ttcgtaagaa atatggctca tccgacacgt ccgctgatta cgatcgttgc agcacctggt
    12121 tctctttgcc taacaggtta tcttaaaaac tttgctgaac ctaattttta tcttgtcgta
    12181 gcattgttcg ctttatctca agtattgtat ttcgttgtgc tgtctatgct tccacgtttg
    12241 ttcaagcttc gtttttatcc aagctatgca gcctttacat tcccgcttgt tatctctgca
    12301 acagctatat ttactactgt tcattacttc aacagtttag gtatctatag tagcgtattg
    12361 gatttcttaa aagttgcgga atgtctgttt gcaagtgtag ttgtcttcta cgttcttgct
    12421 cattacgtac gtttcttaat taaagaacat agcaaacatg gaaaatttgt ttctaaaaaa
    12481 atcgcttaat aatcgattca ataggttgtg aaaaagctgt tctgcatcaa gtaataagaa
    12541 cggccaaaca aaaacagctg ttcatgtttt tgtactggtt gggcttatta ccgcagatgc
    12601 aagcttttga acaccgttta ccaagaggtc gtaaaagaag tggtcagctt caagaaataa
    12661 gaaggaattt acgaaaattg cttctcaaat ttttgtgaaa tttcggctta tttccgtaga
    12721 agctacttct ggaacaccgt ttattcgaaa caagaggtcg tacaagactt ttgtcacgac
    12781 ctcttgttct tttgtcaaaa tactggttta gtgctactat gttcgagtat gcttttagta
    12841 tttattgacg aaaggaaccg atcaaatgaa atcattgtca catccaataa aatctgtaaa
    12901 ggattcagcg gtccgcgaaa tcttacccgg tttactgctt tcttttgcca tcgctgcctt
    12961 cagcaggctt cttgctattt atctgccaca gttaggtgga gcgactattg ctattttatt
    13021 gggtatttta ttaggaaata ctttttttaa acagcctgtt ctagcaatcg gcacaaaatt
    13081 tgctgaaagc aaactattag aattctctgt ttttttacta ggtgcaactg tcacagctca
    13141 aacgattgct caaattggtg taaaaggaat tgtttttgtg ttactgcaaa tgtcagtcac
    13201 tatcatcggt gcttacttga ttggtaaaaa actgcaattc agcgatactt cttctttgat
    13261 gatggctgga ggaaacgctg tttgtggttc ttctgctatt gcttcaatcg ccccttccat
    13321 ccatgcgaaa gaggaagaaa aaggacagat catcacacta gtcaatctac tgggtactgt
    13381 gatgatgttg actctccctt ttcttggatt acagttgttt ggtggaaatc tcattgaaaa
    13441 aagtgcactt ttgggtggta cactccaatc tgttggtcaa gtagtcgctg gggcaagctt
    13501 acttgacaat tctgttgtcg aattcgccat gctatttaag attctacgta ttatgatgct
    13561 cgttgttgtt gtctatctgt ttgaaaaatt catgcacaag aaacaaccag ctctgtttga
    13621 aacgccaact gatacgcaga aaaaacgaaa attaccgctt ccttggtatg tattagggtt
    13681 tgtgatcttt tgtttggtca acagcttgtt ttctttacct gtagttattg accaatcggc
    13741 acattttatc agcagctggt ttgagatcac tgctttagcg gcaattggtc tgcgtttaga
    13801 ctttgcaaaa ttcatgaaag aaggtacacg cttcttatct tatggattag ctgtcgggct
    13861 tctccaaaca gtgatggctt tactatttat ttggctgatc aaagtttaga atgaacatga
    13921 aaaaacagtt gtgattctcg catggaacaa tgtccgaatc acaactgttt ttaattttgc
    13981 agatatcctt ctaacaacaa caattggcgt ttgatttcaa ccccatctat tgcgtttccc
    14041 atatatcctg tcagttttcc attttgcccg atcactcgat gacaaggaat aacgattggc
    14101 aatggattgg ttcgatttgc ctgcccaatc gctcgcacag ctttcggact atcaacagca
    14161 acggctactt ctttatacgt tcggatttct ccataaggaa tggtcgacaa agctttccag
    14221 acattttgct gaaaaggcgt acctctcttt atagataaag gaacatcaaa ttcttttctt
    14281 tttcccgcaa aatattcttc aaactgttct tgtgtgcgcg ttaaaattcg ctgattcgct
    14341 gattcgcttt ctgaaattgg ctgaaaagat acagcagtca atccggattc atctgcatct
    14401 aaccataaaa agccgatagg tgtgtgtatt ttcattttgt ttgctcctag tcaatagact
    14461 ggaacttcct cctttttctc tatccaaaac tacttgccaa ctaataaaaa ttgttcttaa
    14521 aagtttgata aaccgaggaa acatggtaaa ctaaatacgt tagtttacaa aaagaatacc
    14581 tgttctttta cgtaaaatca aattttaaag gtgttattta aggtttatta aattttgaat
    14641 gtaaaccata tataaacata cgattgaaag gatagaaacg atgaaagtat caattgttgt
    14701 cccatgtttt aatgaagaaa aaactgttcc tctttttttt gatgaggttg aaaaattcaa
    14761 aggtgcccat gagttcgaat atttgtttat tgacgacgga tcaaaagacg gtacgttagc
    14821 agcaatcaag aagctagcct cagcttatcc ttatgtccgc tttgtctcat tttccagaaa
    14881 ttttgggaaa gaagcggctc tgtacgctgg attgcaagct tctacaggag atttagtgac
    14941 cgtgatggat gttgatctgc aagatccgcc cgaactgctt cctgagatga tcaaccggat
    15001 cactacttct gatatcgatt gtgtcggcac ccggcgttcg actcgtgaag gcgagcctgc
    15061 gatacgcagt ttctttgcga aaaaattcta ccagttgatc aataaaatca gcgatacaga
    15121 aatggtcgat ggcgctcgtg atttccgctt gatgactcgt caaatggtgg atgccgtcct
    15181 agaattaaca gaatacaacc gattttcaaa aggattattt agctgggtcg gatttaaaac
    15241 agaatatatt tcttatgaaa accgcgaacg tgtggctggc gaaacatctt ggtccttctg
    15301 gaaattattc aactactcga ttgatggtat cgtgaacttc tcggatgcac cgttaacgat
    15361 tgcttcattt atcggcgctt tgtcttgtgt cggttctggt attgcaattt tgttcattat
    15421 tttgcgggca ttgttgtttg gtgatcctac atcaggatgg ccttccatgg tctcgatctt
    15481 cctgttcatt ggtggaatcc aattgttatg tcttggaatc atcgggaaat atattgggaa
    15541 aatcttctta gaaacgaaac agcgtcctgt ttatatcata agagaaacag aaaaagtgaa
    15601 gccaaatgaa ttgaagcctc accaataata tacgtcaaat gaagtaaaca tttttttaaa
    15661 tgaaaaagga ctgtgacaaa agtcttgtca cagtcctttt ttccgaataa atggtgttcc
    15721 agaagtagct tcttcggaaa taagccgaaa tttcacaaaa atttgagaag caattttcgt
    15781 aaattccttc ttatttcttg aagctgacca cttctatcac aacctctttc atatcttatt
    15841 acttcttcat tgctgctttt agtgctgctg ctaatgggct ttcttctggt tcttctgctt
    15901 gtgcatattt tttcagcaag cgtcgttctt catgtttggt cattttcttc ttctgctctt
    15961 ttttatcaag caatttttct gtcgttccat cgtatttaca acggaaataa gcaccgtttt
    16021 taccttcgat gatttccatt ttccggtgac attgcgggca tcgatgatta gagacttttg
    16081 gatcttttct tctgcggtag ttgcaatctg aattggtgca gacatagatt ttgccgtcac
    16141 gtgtgttttt ctcgcgcaga ttggccccac attctgggca tttcttttgg gtaatcgaga
    16201 aatcttgata ttcttgtttg cttgatttga tctcattcac caagcgtttt gtttcttttt
    16261 cgattccatt caagaaggtt cctgcttgtt tctttcctgc agcaatagct tcaaggtctt
    16321 tttcccagct ttctgtcaaa tctggtgtta ctaaagaggg atttaccaat actaataatt
    16381 gcttaccttt aggagatact tgcagtcctt gaggggtacg ttccatcagt tcagatttga
    16441 ttagtttctc gataatctct gcacgcgtag ctggggttcc cagactgtat ttttccattt
    16501 gaccaagcaa gcttgcttct gacaatggtt taggcggtgc agatagttct tttttgatgg
    16561 cgaaatcggg attgattctc attccttttt cccatttgac tgtttccgtc tcagtttcag
    16621 tttcgccttt ccatcctgct tggacgacac gattttgccg aaaaatgaat cgttctttac
    16681 caaattcaac cgtaactttt gtttggctga ctttatgggg ttgagcaaac agtcctaaaa
    16741 aacgagagac aatcatttga tagatttttc gctcttcatt acttaatttt tcataacgtg
    16801 ctctgttttc agttggcaaa agtgcatggt gatcggtcac tttactgtct tggaatactt
    16861 ttgtctgttg gacgatggct ccgtttttca aatactgctt gacctcagga gcaaagtcgc
    16921 tgactgcttg caatcgttct ttcatcgtac ttttcatgtc atttgtcaga tatttgctgt
    16981 ctgtacgcgg ataagtaacg atcttatggg tctcatacaa actttgtaca agacttaatg
    17041 ttttctttgc agaatagcca tagcgttgat tggcttctct ttggatctct gttaaatcat
    17101 aaggcaatgg cgcagattcc gtttttaatt tttcttggac ttctgtgaca gttcctttgc
    17161 ttgtactgat caattgaaca agtttttgtg cttcttcttg tgtttttaaa gcaaattgat
    17221 ttttttgaag cattttggct tgttcttttc cagcttgcaa cgtgatcgtg aaatacgttt
    17281 gtggacggaa ctgctcgatt ttcttttctt ggtctctcac taatgctagt gtcggtgtct
    17341 gcacacggcc tgctgataaa ttatccgcgt acttcacagt taaggctctg gtcacattca
    17401 atcctaccag ccagtccgct tttgcccggg caagagcaga ttcatacaat gcatcatatt
    17461 ctttcgcagg tcttagttgc ttgaatccag ttttgattgc tttatctgtt tgagaagaga
    17521 tccataaccg cttcactggt ttattgaaat gcacatactc taagatccag cgagcaacaa
    17581 gttccccttc tcttccggca tctgtcgcaa tgactgcttc tgagacatct tttcgctgag
    17641 ccagctgctt gattgctttt aattgatgtc ctgttttagg aagtggtttg attcctagtt
    17701 ttttagggat cattggcaac gtgtccattt gccagctctg ccattcctta ttcagatctt
    17761 ccggcatttt caatcctaat aaatgaccaa gggcccaagt aacgatcacc ttgggtcctt
    17821 cataataatt tttgtgcttt tgattagcac caagcacctt gcttaggtct ttcgcaacgc
    17881 ttggtttttc cgctagaatt agttgtttca taaaattcct ttctgctttc tactgtgatg
    17941 ggctgatgat ttttcaacaa cgcaagtccc ttgtattgct gtaaatcatc gtcacactct
    18001 tcacaccagc gctcatctcc gtcattccaa aaggcgctga gaaagttact ttttgcattg
    18061 atatattcat aattttgatg gatttcgttc cattgttttt cgtacgcttg tcgtgcttct
    18121 tcgaaagact cgaatgtttc ttcttcttca atatcttctt cccagtcttc aaaaaaccac
    18181 caaggctcat tgtccccgaa catggtaatt acttgataca tactcgtgct ccttctttca
    18241 gcttttctag actcatctta gtcacttttc aagaaagttg caagaatttt ctcttataaa
    18301 agggtcattt aatgaacatc ttaaaattgt tacaaatgaa tctgattgtt taaggcaaag
    18361 tagcgataaa gttattgatc atccaaaaat agctgtcttc tggatcgatt gttggttctt
    18421 ttgtaaagta tcctaaaaaa cgtaaggaaa gataaccgtg gatcatacta cgaagttccc
    18481 tgcttcgatg aactttttct tctttcgtta attgaaaagc ctcaaatatc tgcaaaatga
    18541 tctgattcgt ttcatggatt ccatctaata aaacagcttc attggtatgc ggtacactga
    18601 tcagtaactc atacactgct tgatttgaaa aagcaaagga tcgataagtt tctgcataga
    18661 tctgcagtgc ttcctgacca ctttttccca caagagcccg tcgcagctgg tcattaagtt
    18721 cctgcagaag tttcgctgtt agcgctgttt tgacatcacg aatatttttg aaatggttat
    18781 acagcgaggg agccttgata tccaagcgtt tggctaaatg ctgaaaggtt atttgctgaa
    18841 catccatctc ttctgctagt tctcgaaagc agtcgatgat tttctcttga gacagatttc
    18901 tttttttcat ttccatgttt cctctcataa aataaatcct tcactctttc agtttaccac
    18961 gaaaaagaaa gagaaaaaag agactttcat tgataaacta catattgtag tttataataa
    19021 agagctgtag atgaaaattt tcagaaagaa ggagtctaat tggctgcaag aataaaggaa
    19081 aaattattta ggaaaagaga aacttgggaa caaaacttgt ttgtcttatg gtttggaaca
    19141 tttatggctg gtatcggatt tagtttagtc atgcctttta tgtcacttta catagatacg
    19201 ttaggtaatt atagtacgtc acaattgaac ttttggagcg gattgacttt ttcatctact
    19261 ttcttggtga ctacgcttat ctctccttgg tggggaaaat tagccgacca aaaaggaaga
    19321 aaattgatgc tgttgcgtgc ttcattagga atggcaatcg tcatttcttt gatgggttgc
    19381 gtgactagtg tgtatcaatt agtcgcattg cgtctgcttc aagggatttt ttctggttat
    19441 atcagtaatg ccactgccct tgtcgcaact gggacgccaa aagaaaaaag tggtcaagtt
    19501 cttggaacct tagcaactgg ttctgttaca ggaacattac ttggaccgct tcttggcggg
    19561 atctctgctt cagcgtttgg ctatcgccct acctttttca ttacagggat catcttattt
    19621 ttcgtcttct tgttgagtct gtttttcgtc catgaaaaat ttgtcccagt gaaaaaagaa
    19681 aacatggttt ctgccaaaca gattttcaaa gacttgaaat atccacacgt cgtcatcggt
    19741 atgttcgtca caacgatgat catccaagca tccaacaatt ccattagtcc aatcatcagt
    19801 ctgtacattc gccaattgct gcatggacat gggaatgtta cgttgatcag cggaatcatt
    19861 gcttcgatac ctgggattgc cactttgata gccgctccaa gatttggtcg cttaggtgac
    19921 aagattggga gtgaaagaat acttgcgatt ggtttgggtt ttgcgattct ggtttatatc
    19981 ccaatggcat tcgtcacgaa tgtttggcaa ttagctgcct tacgtttttt gattggtatc
    20041 tctgatgctt gtctgctccc tgctgtccaa gcattgatca caaaatattc gcctcatcaa
    20101 gctgctggac ggatcttcag ttataaccaa tctttccaag caactggtaa cgtagtaggg
    20161 cctatgatcg gctccagtgt ttccagtgca tttggttatc gcggcgtctt tctttcgact
    20221 tctcttcttg ttttggtcaa cttcttgctt gtacgacaca atacacaaga gatccatcag
    20281 caagaaactg cagaagaaaa agcttcagga gtctccatag cttcagggca ctctccttct
    20341 catagacaat aagcgtaaat gaaaaacact gcttgaacag ttgtcacaga ctgtccttgc
    20401 agtgtttttg tttaggcctg ttttttcaag aactcagata agaggaataa tcccttttcc
    20461 aaggtttctt ttttacaaca gtaacctagt cgggcatgct tcggtaaatc aaaggcactt
    20521 cctggtacta atagtactcc tgtttcttct aataaattga tacaaaatgt ctggtcatct
    20581 tcttcgatgg ctaatttaat gaaagaagta gagacgtaat tgggagcgac aagttcaact
    20641 ttcggttcat tggctaccca ttgttttaaa atcacaaggt tctctgtaat gatctttttg
    20701 tttcgcgcca atattttttc tttattcttc aatgcatgta cagcaagatc atctgacagg
    20761 actccgccgc aaatcatcgt gtagtctcga tactttcgaa aaacttctgc caactcaggt
    20821 ccagtagctg tccagccaat acgaatacct ggaatggagt aggttttgga tagggaattg
    20881 gttgctatac ctttttcata gacatctacg atcgacaaga attcaccttt atccgttaaa
    20941 ggcgcatata cttcatctat caatacatat gcatctactg tacgagcaat ttctgcgatt
    21001 ttttctaaag ttttccgatc tagcaatgtg cctgtaggat tgtttgcact attcaaacag
    21061 atgatttttg tttcaggttt gactaaggct tctagctgtt ctaaatcaaa ctgccaatct
    21121 tcttcttcct tcaaatgaac aaagtcaaca gcggcaccta aagatttggg gatgtcatat
    21181 aattgctgat aagtaggaag catcgaaaca acatggtctc ctgcttccac taacgcgtat
    21241 agagctaaaa gattagcacc agtcgctcca ttcgtttgta agatgttttc cggatcgacg
    21301 gtttgataaa gagaagcgac ttcttgtttg aaaacatctg atccttcgat ccagccatag
    21361 tccaatggtt cgtttttgcg tttttcgaaa aatttactta cgctagtccc atctaagcca
    21421 acgagttcgt ctaatgtcaa tgcttcgatc gagctttgtg caatatcata taccgctttc
    21481 ttttcccatt tatttagcca ttcttctaca ccgaaatttg cgattttcat gtttctttcc
    21541 cccttatatc ttgatccagc ctacagcaag caacccgatt gaaagcagtg cggccacaca
    21601 aatcagccca atcattactt tttcttttcc cgtaatttga tgtttggttt cacgacaagc
    21661 gatccagtag aagcaaaaac caggaagata ggcaatacta cataaaagga cataagaaaa
    21721 tcctgctaaa atgatgccca tcaattcaaa aactacagca atcccaccga tcactacttg
    21781 ttttttgatt ccttctttta aagaaaactg gacttgatag atcccgacaa gtagataaca
    21841 aatcaaaatt gccgctgtac ataaagaata ggcaaattga taggcataat cagtaactaa
    21901 gaaagtcaat aagaatagat tagtcaagat tgcagtaaca actaacgaat aagttggggc
    21961 atttttttta ttcgtttttc cccaaccttt cggcaacaga ccgtctcgag ccatcaacat
    22021 cgttgtttct gctgggagca tcgtccaaga caaccaagaa ccaatgatcg aaataatcaa
    22081 gccgatattg atcagcgctg ctccccaatt tcccacgatt ccttttaaaa tatttgccat
    22141 agcaggttgt gaaatagttg ctaactcttc ttgagataag acgccgtatg gcaagatcga
    22201 agcaaggata tagataaaca ataaacaaag cagaccaatg atcgatgctt gctgggcttc
    22261 tgaacgtttc ttcgcgcggt tggctaaaac acttgcgcct tcgatcccta caaagaccca
    22321 catcataacc atcaaacaac ctttaatctg caaccagatc gttccttgct gccctgttcc
    22381 attcactaga ttatcagata cgttacccca aaaatcagca gtgaatatat tcactttaaa
    22441 tgcgataaat acaaatatta aaaataggaa taatggcact aatttacaga tcgtcacgat
    22501 tgtattgatg acgctcgctc cttcaactcc attgtttact aataaagtca gaccccaaat
    22561 aaaaatactt gcaaataaaa tagaaggaac attttgtcca cctttgaata tcgggaaaaa
    22621 ttctcccatg gcgctcatca gcatcgttgc aaaagcaaca tttcccagcc acgcagacaa
    22681 ccagtaaccc caaccactga tgaagccacc taatttacca aatgaagctt ccgcgtaacc
    22741 gaaaatacca ccatctagat ctggacgctt ttctcctaaa tgattcagcg ataataccaa
    22801 agctaaaata ccaatcccaa cgattatcca tgaaaggatc gctggccctg gagctgcaga
    22861 actagccaga tcacttgtga tcccaaacac accgcttccg attgacgaac tgacaaccag
    22921 agcaaccagt ccccatttgc taatacccgt cgatttcttt tccatttcca tcaccctcac
    22981 ttataaaatt gaacaaatca cttgcaaaag attcccttct attttgcaac tgctcttgct
    23041 taacaaaaag caactttatt catattattg gatattatga actttttaca caagtatttc
    23101 aagtataaaa tgaataaaaa ttaattttat tgcaaaaaaa cagctatgct atccaaaaga
    23161 aaaggtgttt cttttgttag ccatagctgt tttgattcat ttaatatcgc ttaaacgtat
    23221 tgtaaaactt ttgtttcttg atatgcgtgg tcgatcaaac caccacccag acattccatt
    23281 ccatcataaa atacaactgc ttgtccagga gtgattgcac gtacaggttc gtcgaatacg
    23341 acttctgcac gattatcatc caataagcga actgtcacag gaacgtcttg ctgacgataa
    23401 cggaatttcg ctgtacactt gaattctttt ggcattggtt cattcgtcgt aaagtggatt
    23461 tcacttgcat ccaagcttgt ggcatacaat gcaggatgat ggaacccttg ccccacatat
    23521 aatgtgtttg tcgctagatc ttttcctaca acaaaccagg gttcttgcga atctcctccg
    23581 ccaccgatcc ctagtccttg acgttgacca atcgtataat acatcaagcc agcatgttgt
    23641 cctttgactt caccatcaag agtgaccata tttccttttt ttgctggcag atagttgctc
    23701 aaaaattgtt tgaagttctt ttcaccaata aagcagattc ccgttgaatc ttttttctta
    23761 gcagtcgcaa ggcctgcgcg ttcagcaatt gcccgtactt ctgatttttc catgccgcct
    23821 agagggaaca tcgtttttga aagctgttct tgtgataatt ggctaaggaa gtaagtttga
    23881 tctttattat tgtctactcc acgaagcatg tggaccacgc cgttttcatc gcgagtgact
    23941 tgcgcataat gtccagtagc tacataatcg gcgccaagat ccattgcata atccaagaat
    24001 gctttgaatt tgatttcttt gttgcacatc acgtctgggt taggtgtacg tcctgcgcgg
    24061 tattcggcta ggaaatattc aaatacacga tcccaatatt ctttttcaaa attcacagag
    24121 taataaggaa tcccgatttg agcagctact ttcgctacat ccttataatc ttccgtggcc
    24181 gtgcatacac cgttttcgtc cgtgtcatcc caatttttca tgaatattcc gatcacgtcg
    24241 tatccttgtt cctttaacaa aagtgctgtt acagatgagt cgacaccgcc gctcatccct
    24301 acgacaacgc gtgttttgct gttgtctgtc atgatatcac catcatttca attagattct
    24361 gaaacatcta cgagttgaag gtcgcaactt ttcagtattt tacattatac gtcatatttc
    24421 tgaaaatttc atcccttaat tatcaagtcg taaaaatact gttctactgg acctattcag
    24481 tacacaaaca tgatagtcaa ccaaaaaatc actcatcttt tgagcattcg atataaaaaa
    24541 ttggcatact cataaaaagg gccagccttt cggcaataag gaataatttt ataaattgtg
    24601 aaaaactatt tttcaaaatt gcttcttatc gcttaaagct gcacgtctca tttacaacct
    24661 tttcttttta gatgttatga gttgctcaga taagtgattt tgtttactct aagaaaaaag
    24721 ggaggcaaaa gatacatgaa ttactttatc agtgatatgc actttttcca tgaacgctta
    24781 ttaggaaaaa acgactttgc tccgcggctc tttgcaaatg tggatgaaat gaaccaacag
    24841 ttgatcacta gctggaatgc agtaataaaa gaaaccgacc atgtttatca tctaggcgac
    24901 cttgctatgc atccgcaata tgaaaaagca aatgcagaaa ttttggaact ggtcactcaa
    24961 ctgcaaggaa caatccactt gatcaaaggg aaccatgaca gtcgcgcttt tttccgttat
    25021 ttagaaaaaa atgatcctag gcttactgac ggaagtccca aattttattt ttatgatgta
    25081 ggtgccatcg ttaaattcga ccatcaacaa tattacttga ctcattatcc gatgttgctc
    25141 gggcctaacg acactatccg caatcttcat ggacatatcc atcattacag cgtacctatc
    25201 gagaacgatg tcaatgtagg cgtagacgca ccggaaagag aactcttaaa ggaaaaacta
    25261 ccatttggca ctccgttatc tgagcaacaa atcaaagaaa tctatgaagc aaagaggaag
    25321 tacgataggt cgtgaaaaaa ctgccctata tcaagtatta aacaaataaa gataattact
    25381 catatttttc atttgagtag gcttattgcc acaggaacat taggatcaga aatggagttg
    25441 taacaagact tctgtcacaa ctccattcca tactattcgt ctttttgctc atggtgttct
    25501 tttaccagtt ttttctgtaa ttctttatca taatttcggg cagctgacgt taaaaataaa
    25561 acgattccgc ataataaaat cccgattccg gaaaacaaga acattttttc tacccctaaa
    25621 cgatcagcta atggtcctgc aaataataat ccaattggac ctgccaggtt catcaaagag
    25681 ttgaagttcc ctaagacacg acctaaaaca ttcgattcat aactctgctg gatcattgcc
    25741 atcagcagtg tattgaaata tggcgttgca caacctgctc ctgcattcaa gataagaaag
    25801 tacaagaacc cttgactatc gtttggaacc aatccgcttg caccgatcgt caggcccata
    25861 atgatgtagg ctataataat aggtttcatt cggtctttcc atgttcctat aaaactgatc
    25921 agtgcaccgc ctaagagcat tccgactgcg tagactactt ccactgctcc cgcctgtcca
    25981 acggaaccac caaagtaatc tagcgtcatc aatggataca gactgattgc tggcatgaac
    26041 aacagcatca caaatgcacc attgatcgtt atgtaccata accctttatt atccatcaat
    26101 tgctgcagcc caaattttgc atctttaaaa atagtaagcg gaacttccaa tatttcagga
    26161 gaaacttttg ggatcttcac aaaaatcaat aagccgacac caaaaactgc ccccaataca
    26221 tctaataaaa taaggtaatt gacaggaacg actgaaaaca aagccgcacc tagagctggc
    26281 gcaatgatga aattcgctga ttggaccatt cctaactgtc cattcgtcct tgttatgtga
    26341 gaagatggaa cgattgtcgg cataatcgac tggatcgttg gcatttggaa tgtttgtgcg
    26401 accgaacgca taaacaaaga cacaaaaact agccaaatcg ggaaggattc tgaaatcgtt
    26461 ccgactactg ctaaaataag cgcgaaaatc gctacaataa tatccgtgac aatcaaaaga
    26521 gcttttttat cccagcgatc cactagcggt cctacaaatg ggcttagtaa aaccatggga
    26581 atcattccta gtaaagtagc aaaactcaaa attgtcgccg aaccggtttc tcttgtcaaa
    26641 taccagatga tcgcatattg gacgaccata ctagtgattc ctgacaaaaa ctgtcccgat
    26701 aaaaagaaca aataatttct cttccaattc gttaaaacga ccgactgttc attttccata
    26761 aacatccctc ttttcttttt cattttgtat agcgtttgca caacaatcat tatacgggat
    26821 tcttttttca aaaaatacct ttttccctaa ataacaatcg tttcaattta aatttcttta
    26881 cttagaaaca ataaatcagc aaaatattaa aaataacaac atgtgttaaa tttaaaaata
    26941 aaaaagacaa ctaatttgcg tcgtccttac aaagttagtc atctaacaat aggccgtttc
    27001 aaaactagtt gtctttttat atccacagat cattcgactt tttcgaataa cccacggcta
    27061 atcatcccat ccatcagaaa ataaaattta tcttggctca ttttctgttt tggatctttg
    27121 aaaatattga ctgctttcaa tccatttgct gtagtgatcg acattttcaa tgtttgaata
    27181 tcttttgata cagtgatttt taatttgaat ccttctttgc tttgcggcgt ggcatctaat
    27241 ggtcggatca attgataatt gcctccgttt gtttctgtaa atccattatc gcgcaatgta
    27301 tatctttttg catcaggatg caaacgatag ctaattggtg cgcccagtac cgttgctgtt
    27361 gtttcaaatg ccatgatttt cacctcgata aaattagttt ctctttatta taaaagttta
    27421 tcctgatttt tactaggttt ttcagttaag ttccctaaaa aatgaaggcg ttctctcaga
    27481 catctgttca tataagaatg acttcactag ttattttttg acagcacgaa ctaattgatc
    27541 aaggaaatac tggatgtcgt cttcggtatt tcccaaacca aagctgatac ggatggattc
    27601 ttttagtatc ggtgtatctt ctccatacat tgcttctaat acgtgtgaag gctcgatatt
    27661 tcctgctgta caagctgaac cagtagaaat agcgacccca ttaagatcaa gcttcatcag
    27721 tagcaaatca tttgcttcgc caattaagcg aagattcaac acatgaggga gtttattcgt
    27781 taaatcacca tttacttgat aagggatttc gtttgcttct aatgtatcaa gaatcaattg
    27841 ctggaatcct ttatatttgt tgttacgtgc ttcacgttct tcaggtgaca gcagttcaac
    27901 tgcttttgcc ataccccaga tccctgctaa attttctgta cctgcacgcc gcttttcttc
    27961 ctgctccccg cctaaaagca atgcagggat atttactccg tctcgtttat ataagaaacc
    28021 gacgcctttt ggcccattga ttttatgagc agagatactt aataaatcga ttcctaattc
    28081 ttctggatgg atcgtttggt ttccgtacgc ttgtaccgca tctgtatgga aataagctgg
    28141 atgatccgct aaaagttcac cgatttcttt gattggcatc aaatttccga tttcattgtt
    28201 cccgtacatc acagagacca agatcgtatc ttctcgtaac gcttttttga cctcttcgat
    28261 cgacagattc cctttttcat ctacaggcag atatgtaacg tcaaacccct ttgtctctaa
    28321 atatttcatg gtattaagaa cagcaggatg ttcgatagct gtagtgatta gatgtctgcc
    28381 ttctttttga cgcgagaacg ctgtttctaa gatagctgta ttatcccctt ctgttccccc
    28441 actgttaaag atgatctcgt gaggttttgc ttgcagactg tctgcaatga tttgtcttgc
    28501 tgtttccaat ttttcatgcg cgtggcgacc aaatccatgt atgcttgaag gatttccaaa
    28561 cgtgtcttgc atgattgccg tcatcgcttc aattacttgt ggatgcatgg gggtagttgc
    28621 cgcatgatcc agataaatag ttttcacttc attcatccct tccaaattat tgctaggcac
    28681 attctagcat aaaatagcga acaaaccaaa gaagagctca tttttgtcta gaggacgttt
    28741 catgggcata gaaaaaagga gctgtgacaa aagtcttgtc gcagctcctt gaagctgctc
    28801 acttctgtca caacttcttt ccctcttttt tttgtttatt cgaattcttt ttcttccaga
    28861 cggaataacg gactcatcgg tgtattttca tggatgcgtt tgaccgcttc acccattaat
    28921 tctgaacatg taacgattgt taagttttct ggatgacgat tttcttctgt gaaaacagaa
    28981 tcagtgatac aaatatcttt gattggtgca ttgtctaaaa tacttttcgc atgatcggac
    29041 aataatccat gtgacgcaca ggcatatact tcttttgcgc cgttttcttt taatacattt
    29101 gctgctcgtg caagcgttgc tcctgtgttc aagatatcat ctaccatgat acatgtcttt
    29161 ccttgaacat caccgatcac ataaccaccc tcagttgaag catcatctgc atgatctacg
    29221 atggctaatg tgctgttcaa atattctgaa agactacgtg cacgctgaac accgctgttt
    29281 tttggcgaaa caactacgat gtcatctccc gtcagaccta attgacggta ataatgagca
    29341 aacaacggca tcgtaaataa attatctaca ggaatatcaa agaatccttg tacttgaacc
    29401 gtgtgcagat ctaaagtcag tactcgagtt gcccctgctt cttcgatcaa gttagcgatc
    29461 aatttcgcag tgatcggttc atgaggtttc gccgtgcggt cttgcctagc atacccatag
    29521 taaggcaata cgacattgat cgttttcgca cttgctcgct tcattgcatc gatcatgatc
    29581 agtagttcca tatagtaatc attgacaggt gcattcgttg attgaacgat atacacatga
    29641 tcccctcgaa tactttcttc gatattgatg gaaatctcgc catcactaaa ttgcttcacg
    29701 acgcttttac ctaattctgt tccgaacact ttcgcgattt tttcagctaa tggacggttg
    29761 ccattcaaac taaaaatctt caacgtgtca tcctgatact tattactcat gtagtcctcc
    29821 ccaaaacttc tctattcttc gatagtttaa catatttttc caagattttc tgttaatttc
    29881 agcaaattct taaggaagtg aaactgttcc gttactccaa ctggcaggaa tgactttatg
    29941 ataatcgccc cagttgtcgt gtactttgta ataacgttct cctgtttcag tttcaaaata
    30001 agcgtacgct gtcacccaat gattgccata tgtactgcca agtactttca aaataccaat
    30061 catcacaggt ttgccttcac gtatacgttt tgtagctcgc tgccatgaac ctagaagagt
    30121 tgctcgtgca cgctcatgat tgccattctt ctttaaaaaa ctagaaatcc ctgtactaac
    30181 ctgaaaagga atcgtaggaa gtcctagtgg ttggatctgg ctacgcaatg ccttcgtcaa
    30241 aaccaacgaa tcagccgtat ttttcttgcg aatctcattt ggaagcatcc accccttacg
    30301 atagtcttga taataggcaa ggagaactgc tgccgcgtaa gtaccacaaa gatagcctga
    30361 gggggtagcc catttcttat acctcaaaaa acgttcttct ggcaatccca cccagcaatt
    30421 tgcgagttcc tttcctgacc ttttttcttc gatcaaaacg ggaatatctt cttttctgtt
    30481 gatccatcta gataactcga ttggctcttc attgatcagc ttccattttt tacaagaaag
    30541 accctcttga taagtatatt ttgctggaga atattctacc ggatgtcctg attgatcgat
    30601 aatcacataa ctttgatctt caaaaaccag agctaaaaaa agcaatttac tttttcttga
    30661 ctgcagctgc tgagtgctgg ctaatttttt aattttttta tctactttga aatataaagg
    30721 tttatccact acttgattca gttgggatcg cctccttttt ttcattataa gtgaaggatt
    30781 gaaaaaaaca aatcagtttg attgttcgaa ttttcaattt tttatgttct atttgttaat
    30841 tccttcattt tgtaagataa tgggcacacg aaacaaaaaa ggagcgatcg atgaatgaac
    30901 aaagaacgat tgaaacaaga tttctatgaa gcagtcaatg aagaatggca aaaaacagca
    30961 aaaatccctg cagataaacc agctacaggc ggtttccaag atttagttga cgggatcgac
    31021 caacttttga tgggagaaac agacgatctg cttgcccatc cagaaaaagt gtcttcagaa
    31081 aaaatgaaac atttccttgc atattatcaa ctggcaaacg attatgaccg aagaaatgag
    31141 ttgcatgctc aaccgcttca acctataatc gaaaaagttg aatcattgca gtctttcgac
    31201 gaattgaata agcaatttcc agaatggctg ttggatacat taccattgcc atttaatctg
    31261 gatgtagatg cagatatgaa gaatgcgcaa acgaacgcct tctttgctta tccgccttct
    31321 ttattccttc cagataagac gtattatgca tcagaccatc ccaacggtgc acaactaata
    31381 ggcgttttct ttgatatggc tgttcagtta ttagagcttg ccggcaaaga aaaaagtcag
    31441 gcagaagcaa tcacggaaca agcagtgcaa tttgataaat tgattgctcc tcatgtaaaa
    31501 agtgcagaag aaaacgcaga ttacagcaaa atgtataatc cgcaatcttt tgatacattt
    31561 attaaataca cagataaact ggacttgaaa aaacttgtca cgggtttgat tgggacaact
    31621 ccagataaag tgatcgtaac tgatccagtg tacttcgatc atttgaatga catcctgaca
    31681 gaagctcatt ttccgttatt caaaagctgg atgatcatta aaacagtacg ttctcttagt
    31741 ggttatctat cagaagaatt ccgccaagtc agcggaatct attctcgcgt attgtctgga
    31801 acagacgaag caatgcctca gaaaaaagca gcgtattacc ttgcctctgg acaattcgat
    31861 caagtagttg gcgattacta cggaaaaaaa tattttggcg aagctgctaa aaaagacgta
    31921 gaacaaatgg tgaaaaaaat gatcgccgtt tataaaaaac gtctagaaga aaatgactgg
    31981 cttggggatg caacaaaaga aaaagccgtc ataaaattaa ataacttagg tatccaagta
    32041 ggctaccccg ataagatccc agcattattc gaccagttca aaacagtacc tgcagatcag
    32101 ggtggtactt tgctttcaaa tactcttgtt ttcagcaaac tcacattaaa agatcgtttc
    32161 agcaaatgga acaaaccagt ggatcgttca gaatgggaaa tgagtgctga cacggtcaat
    32221 gcttattatc atccatttcg gaatgtgatc gtctttccgg cagcgatcct tcaagcacca
    32281 ttttacagtc tagaacaatc aagctctgct aattatggtg gaatcggagc tgttatcgct
    32341 catgagattt cccatgcatt tgataataac ggcgctttat ttgacgaata tggcaacttg
    32401 aacaactggt ggacagaaga agatcttaca caatttcaaa acaaagcaca agcaatgatc
    32461 gaagaattcg atggtctgcc ttttgctgac ggaaaagtaa acggaaaatt aaccgtttcg
    32521 gaaaatattg ctgatgcagg cggtctaagc tgtgcattgg aagcagcaaa aacggaatca
    32581 gatctttcta tcgaggaatt ctttgtcaat tgggcaacga tttggcgtac aaaagcaaaa
    32641 aaagaatacc aacaactttt actgcaaatc gatgtccatg cccctgctaa actccgagca
    32701 aacgtgcaac caaaaaacct tgacgaattc tatgaaacat ttggtatcac ggaacaagac
    32761 gctatgtatc tagcgccaga aaaaagagtg cgaatctggt aagaggttgt gccagaagtg
    32821 gtcagcttca agaaataaga aggaatttgg gaaaatcgct tctcaaattt ttgtgacatt
    32881 tcggcttatt tttcgaagaa gttacttctg gaataccatg tattagaggt tgtgaaaaag
    32941 ctgcttagtt tcaagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata
    33001 aatttgactt actaccgaga aactggcttt tgaacaccgt ttatcgagag gttgtgaaat
    33061 cagcgtcctg acctgagtat tagagaaaaa aacggaaaaa tagttcctca tattttgccg
    33121 tttgttgagc taataccgag ggtcagctga ttgaacaccg tttagtggag agattgtgaa
    33181 aaagctgtcc tgcatcaagt aataagaaca gccaatcaaa agcagcaagg agaacctcat
    33241 ctataaaaag atagaaggaa gtgagacatc tattggtctc atttccttct atttttactt
    33301 tcctcagtca attgttttac atcataaaag gagaaattcc acttattttt ttcgctgttt
    33361 catgacataa acaactacac cgattaacaa aattcctcca gctaatgcta gcaacgggtt
    33421 attaaattct cccgtttttg ggagtccttg agtgccattt ttttcacttt cttttgctgt
    33481 cctatccgga tgagaaggtt ttccatttgg ttttccattt ggtgattcat ttcctggatt
    33541 ttctctttct ggtttgtcat ttcccggggg cacatcggca gtttctcgca cagtgatcgc
    33601 tttttgctgg gtaacagatt ctgtatgagc agaccatttt tccccatcaa atatttccga
    33661 acgataagtg acttctagcg tatattctcc ttttgaaggt gctgaaaaaa tagcggtatg
    33721 attagaaaat tgagcacttg actgtgtttc aacgatttgg atcttttctg ggaaataacg
    33781 catatcttca aagacaggcg ttccttgatt tggtgtgaaa gttgacacaa aaactgtaaa
    33841 aggcttcagt tcaatgatat ttccttctgc attctccgcc acaccatttg cttcttgtat
    33901 aataatcgga ttattttgtt catttggttt tgtctgattt ggttcttgta cgattgcata
    33961 ctctggatat tccgaagacg gtgaggtttc tccgtcaaaa atagcaccta cagataatgg
    34021 aagaccatcc actctttcct tttctgtttg acgattcccc ggtttaatct gcatcagtcc
    34081 atgaacatgg atacttccat tttcatagcg cgtaacaggc tgctgacctt ctttcgaaca
    34141 tgtatttaga gactcgatcc aatcttttga tatggtatgt ccaagagtat tttctgattg
    34201 tttctctcgt tcattccaat agtagttcaa cgagtggtag atttgatctt cgttttgatt
    34261 caatgggcgg atctgctctc tcatctcaag attctctgtt cggaaagata agataccaga
    34321 agcatggtag tctgtgtttc ctgctgaaga ggtataaaga gccacattat atgaaccgtt
    34381 attaaaactt gtactgctat tgacgataat atctgttccg ctattggaat cgattccttt
    34441 cgcaagattt tcgaaagaca aactattgat caattcatga gggcccgtta aagaagaacc
    34501 gcccattttg aatccattac cgttaccttc tccctcaatt cctggtaccc agccgtttcg
    34561 ataagcaact gagtttttga tgattacttt tccgatcgaa cctgtttcat ttttagcaaa
    34621 aaggtcccaa ccgtcatctg cattatgata cgcgatacaa ccgtcaaata catttccttc
    34681 tcctattgtc agtttggccg caaatccatc ggcatcttca aatcctggat cggcattttc
    34741 aaaagatgta cagttcttga ctaagttatg cgcgggccat gcttcgaaag gatcatttga
    34801 gctaccgctg atttggattc ctgtatttcc attccgataa gcatgaatct cttcgatcaa
    34861 attatgattt ccagaaatct gtaatccttt ttgcatagct gcactatttg taatgtcaaa
    34921 ccctcgtaaa ccccagtagt gactgaacag tgtcatccca tttccagttc ctttaaaatc
    34981 aagtacagga cgcttgtttt ctggattttt ctctgcaatc aactgaatcg ggttttctct
    35041 tgtcccgtta acgccttttg gaatcgtcaa tcctctttcg aaggaataag ttccaggcgc
    35101 catgacgatt gtctgcccag catatgcgta tctcaatgct tgtgtcagag ataatggatt
    35161 ttgtctgctt ccgtttcctg catctgaccc ttctggtgtt acataaactg tcgaatattt
    35221 aggtttacgg tagtccactg tatgaagaat ctcttttgtt tcataactag tcatctctac
    35281 gtattcttct ggcggatagt ttttatctgg cgtaaaactg aaagtaaatt cgttcgttcc
    35341 ttccatcagt tttgtctgat aatcaaattc ccgattcgca gtgatagcca agttttttgc
    35401 gttcttaagc acctctcctg ttgctttatt tttaattgtc aataatccgt cagcatttgc
    35461 tctaaaggac atagtatgat cttggtttcc tgaatgatta ctagaggtca cagtgtaaat
    35521 cggatcaatc aagcgagtag gtctttcttg cccaggagga tctgtcgcag gatctgagta
    35581 agcaacttcg attccttcta ccttgatcgt catattcctt gctgcagcaa atcctgcata
    35641 aataaccgat tcatctgaag cgtataattt ttcccagtca tacatgatgt cagaagccat
    35701 ttcttctcct gtttcaggat cgtaataata tgcctctaaa ccagtattcg tttttttcat
    35761 ctctacgtcc agtttagtaa tcgagttcgt ggccggaaac agattcccat gaccgtttcc
    35821 gaatatatta taagaacctg tttctttttc caagaaccct gctgaagtct ctaaaggtgt
    35881 tgtttccact gagacactcc caggagctgg cgcagcttgc ggatcttcag atgatattcc
    35941 tgtgattgcc cgtgtcccca atccaagtcg catcgtatat ttgctgccgc tcgtgttgac
    36001 cacttcttgt ctatccgagt cccagacata ttcgatccga ctgcctagta tggcaaatga
    36061 atttgaataa aatgcctgcc caaacaaatg attttttggt actgcgtctc ttaccattaa
    36121 agcaaatcct tcttgggcat tcgtataggt ccaagattct atttcgatcg tcgcccgcat
    36181 cctaaagttg atgtctttgg ggattgcttg ataataatag gtcattccat ctgatccgct
    36241 tgactggaat tttccgccgt tattcgtgga agagagccgc acgccgtctt ctaatggttc
    36301 aatggcatac ctactagcac ttgtgctttc tccgaaaaca acccctttcc actcttcatt
    36361 tgcttcttca gattgctttt ttgtcttttc attttctgat tctttcatca taggatcatt
    36421 tccttcttcc gtatccatat cttcactagt tcttggtatt tcctccattg ttgacatccc
    36481 ttcaatgttc acgtgtgcat tttcttcgtt cacagactgt tgttcgttag cagcaacttc
    36541 aacgacagaa attaaaaccg gggtactgag cataaggaga ttcatcatta gcgcactgta
    36601 ttttatacgt ttttttaaca agtgtattcc tcccgtaaca aattagaaag cgtttactta
    36661 aagtattacc taatttattt ttactgtcaa taaaaaacaa aattataata agtgtatttt
    36721 ataaaaagaa aaaatataac ttaagttgtt gataataaag agatttatta acggaaaaag
    36781 aataagtttt tttaaaataa cgtataaaaa agagcataaa acaaagtcgt ttgttttatg
    36841 ctcttctttt attattttgt gctttttatt ttatcaaatc actttgtttt tttataaaat
    36901 ttctgcgcct agtgtggact caaaatgtct caatgcccat tcatgtccaa cgggatcgaa
    36961 acttgccacg gaatctttga aaatatggag tttgtatcct agattatacg catcgactgc
    37021 tgtatgcaac acacagatat ctgtacatac gccggtcaaa taaatatctg tgatctggcg
    37081 ttctcgcaaa cggatgtcta gatctgttcc actaaaagcg gaataatgcc gtttgtctat
    37141 ccaataaaca ttttcctctt gctcatagtc ttggtataaa ctttttaaag agccgtacaa
    37201 ttcccgtcca cccgttccta tcacattatg cggaggaaat aagcggttct ccggatgaaa
    37261 ggaatctttt ggatcatgcg catcaatggc aaaaacgaca aaatcctttt gatcaataaa
    37321 cttctttgta tgagcagtca gctttttttc aattacttgt cctgctgtcc cagtagtcag
    37381 ctttccatcc tctgcaacaa aatcatatgt gtagtcaatc gagatcaatg cgttcatctt
    37441 agtcctcctt atacgagatg tttgacaaat tcgatcacca tttctgctga tcgtttgcct
    37501 gcgtcttcga taaattcgtc aaagctttgt gtcgccgagt gatctgctgt gtcgctcatt
    37561 gcacgtacga tcaaaaatgg gatattgaac tgacgagctg tttgtccaac agcggctcct
    37621 tccatttcgc aagctaatgc ttcagggaaa ttcgttaaga tttctttgat tttatctggc
    37681 gagtcgacaa atgtatcacc tgtaacgatc aatccttctt ttgcgttcaa attcgttgct
    37741 tttgctgctt taaccatttc actgcgcaaa tatgtgcttg cttcataata tagaggcatt
    37801 cctggtaatt gtcctgggtt gtagccaaat cctgttgcat ctgcatcaaa ataagctact
    37861 ttgtctgata taacgatgtc acctacttgt aagccttcac cgattccgcc agctgaacct
    37921 gtattgatca ccatgttcac gccgtattgt tggatcaaca gacttgtggt aatagatgct
    37981 aagacttttc ctataccgga acgtacgacg atcacttcgt ggttacctaa tgaacctgaa
    38041 ataaataaag cgcctgcgcg ttcccatgat aatggttcgc ttaggttctc tctcaaaatt
    38101 tttacttctt cttccattgc gccgataata ccgattttca tctttttaat tccttctttc
    38161 tatataaatg cgattgctaa aaaaaccaca cataacatta ggataacaat aataagagat
    38221 ttcatcaaaa aagagttcca tttggtcgtc ttttcccgtt cgctgatcct tgttttcgtg
    38281 attggtttat tctttttgct ttctttacga taaaagctgg caatcttttt ttcttcttgc
    38341 tgataagctg tttctgcttt tcgctgtttt tttaatgact cgcttgcttg tgcttgttgt
    38401 cgcttgcgaa gttcgcttcg cgtaataagc ggtcctttgg ccataagccg atccctctac
    38461 tttcctttgg tcaatagttc ccagtattga atcgcataaa tcgtctttgc atcacaaatc
    38521 aactgatctt tcatcgcttg ttgcgcctct tcaagagtta gatgatacaa ctccagtact
    38581 tcgtcttcat cttgtgctaa aggattctcg accttctcta ccccttgtgc atgataaata
    38641 tgaaggactt catttgcaaa acctggtgaa agatacatcg aagtaagatg gctcagcgac
    38701 ttcgcacgat agccagtttc ttcttctaat tctctagctg ctgtcatttc gggattttgt
    38761 ccttctcctg gatcgatttt tccagctgga atctccaaaa tcactttttc taaaggtttt
    38821 cgaaattgtt tgacaagaag cagacgatct tgttcgtcaa aagcaatgat cccgacaccg
    38881 ccaggatgaa ataccagttc tctcttcgaa gttcctccat ctggcaaacg aacttcgtct
    38941 acagcaacat caataatttt tccgctatag atttctttgc gactgattgt tttttcttca
    39001 aattgctcat tgttcagcat atcttctcct gctttctttg taatcataac tgcttttttt
    39061 tcatttttca atttttctca aaaactcctt attagtttac catagataaa ccttagattt
    39121 tctctaaata tagaaaatcc ttctttagtc cgatactctt tctagtagtc cgtatgctaa
    39181 aataagtatg taatcgaata acgaaaagaa ttcaatactc aacgataaat gacatggagg
    39241 gtttattatg ataaaacgaa attggccgtg gcttctagca ttggtcttgc tatttctctt
    39301 tgtccgaaac aatagttttt cttttgttct aggactttta ttgataggtg tagttttagt
    39361 tttgtgggga ctctttggtt cgcgcaggaa aaagggaaaa caagaagaaa ttcctgcact
    39421 gactgacgaa cttgaaactc actatgaaaa aagcggtatg acagcgaatg aaatttcatt
    39481 ttttcgacaa acaatgaatc aaacaaaaaa tgagatcaat caattgcaaa tgaatatgca
    39541 gcagacagcc aaacttaaag caatcgatct ccgccatgat ccagtcaaag cagcgaaagc
    39601 tttattcaaa gaattggtga aagaaccaaa ccgactgcat gaagcaagtc agtttcttta
    39661 tacgcatctg ccgaatctag ttgatttgac aaataagtat aatgagatca atgaccatga
    39721 gatcaaaaac aaacagacct atgaaaaact agaagaaagc gcgcagatca tcgaccagct
    39781 gtcagcttta atcgcacaag attatcaaaa attcgttgct gaggatctag atgatatcga
    39841 cgtagagata tctgttgcga aacagagctt aaagcgtgat aataaatatg aaccagaaca
    39901 aaaggattaa ctcttttgtt cataaaagaa gactcggaac attcagatga tgtctggctg
    39961 tttcgagtac tttttctaaa gtagagacat tcatgtaaga atcgagaaca gattcaacaa
    40021 ggaggaattt tttatggatc ataaaccgac tgaaacaaaa gatgttacac cagttaacga
    40081 tacacttgaa gatttattga acaacccgtt ttcaactcca acagatacgc tgaccacttc
    40141 acaacaagcg gagattgatc agctccaaga gcaacagaca gctgctcgtt taattgacaa
    40201 actaccggcg gaacgccaag aacaagctaa acagctagca gcaaaaatcg atgctaatga
    40261 tgcacagtct gtcatcagct acggatcggc tgcccaagca aaattaagtg aattctcaca
    40321 atcgatgttg aatcatgtgc aagcacaaga tattggtcct gtaggagatt ccttaactga
    40381 actgatgtat cgtctccaag aagccaatcc tgacgaactg cgtgccggag aagggaacat
    40441 cttccagcgt gtcttcggga aggtcaaaca atcgatctat gaagtgacag caaaatatca
    40501 aaagattggt gcacaaattg acaagatttc agtgaaatta gataaagaaa aagacggtct
    40561 gctaaaagat aatctgatgt tagaacagct ttaccagaaa aacaaagact actttgatgc
    40621 actgaatatt tatatcgctg ctggagaatt gaaaatggaa gaacttcaaa caacgatcat
    40681 tccagaagcg atgaaaagag cagaagaaac tggcgatcag atggatgttc agattgccaa
    40741 tgattacacg caattcctag atcgtctgga taaacgtacg catgatcttc gcttagctag
    40801 acagatcacg atccagcaag caccacaaat ccgtttgatc caaaacacaa atcaggcctt
    40861 ggctgaaaag atccaagcat ctatcgctac agccattccg ctgtggaaaa accaagtcgt
    40921 gatcgcattg accttgcttc gtcaaaaaga tgcagtaact gctcaacgcc aagtctctga
    40981 aacaacaaac gacttattga agaaaaattc agaaatgctg aaaatctcag ccatcgaaac
    41041 agcgaaagaa aatgaacgtg gaatcgtgga tatcgagacc cttcaaacca ctcaaaatga
    41101 tttgatcgaa acaatccaag agacattgcg tatccaaaaa gaaggaaaag aaaaacgtcg
    41161 tcatgcagaa gtagagcttg gacacatgga agaagatctg aaacaaaaac tgttggattt
    41221 aacccaatag atcacctttc gcttgttact aaaacaaacg cccaacaaca gtttattaca
    41281 actgttgatg ggcgtttttc ttatctttct tctaacttct ccattggagt ctatttctca
    41341 ttcatcttgc tgtgtccata gccatagcca aaataaatga tcagtccaat aaccattacg
    41401 ataccgaaca cgatccatgt cacagtttgc agacgaagca tcaatacaat acaagtcaag
    41461 atggagacga ttggcaatag cggaacgaat ggcactttga attctccagc ttttggtttg
    41521 ccaaacattt ttctcaactt cagtaagcct agactaacaa gcatcaaata ggcgagcgcc
    41581 atgatattcg ttagttccgc taatacattc aatgggaaaa atccggcaaa gaatacagct
    41641 gccacaccag cggcataagt cgcttttttc ggtgtacgat gcttttgatc gatttctgtc
    41701 ataaacttag gaagtaagcc atccttgctg acagcaaaca gtaaacgagc aagtgaatac
    41761 atcatcgaga tcgttaccgt cagtagcgtt aaaattgccc ctacagcgat caccgcgccc
    41821 actgttccat gtcctacata cctcattgca aaagccactg ggtcattgac tcctagttca
    41881 gtatacggaa cgattcctgt caagatcagc gtcacgatga tgtaaaggac agtagcaatc
    41941 agtatcgaac caatgattcc tcttggcaca tctttttgcg ggtttttgac ttcctcggct
    42001 gccattgcaa cggcatcaaa tcccaagaaa gcaaaaaaca caagagctgc accatctagc
    42061 acaccggaaa aaccaaaagg catgaatggc tgccagttat caggtttaac gaaaaatatt
    42121 cctacaatga taaacaacgc gatgatacca aatttgacat ataccatggc attattcaaa
    42181 cgtaatgctt tcttggcttc aagcgacacg atgtaagtga cgaatatcac tacgagcacc
    42241 gcaatcaagt caatgtatgt accattttcc ggattgtacc cgccagttaa tgcttgtgga
    42301 agttcggccc ctaaactttt caaaaaacct tgcatatagc cagaccatcc ggaagcaaca
    42361 gaggaaaccg ctagtaaaaa ttccccgatc aacaaccacc ctgtcagcca ggcagcaaat
    42421 tcaccaaata caacatacag ataggcatat ggcccaccaa tcactggcac tcgcgaagca
    42481 aattctgcga aacttagtcc agacaaaatg acaacgattg cagctaagac aaatgaaagt
    42541 gacagtgctg gaccagcatt ttgattggcc gcaacccctg tcaccacaaa tattcccgta
    42601 ccgatgatcg caccaattcc aagcatgatc aaatcagacg tttttagttc ttttttcatc
    42661 tcactcgtat gattgagatt gatctctttc ttacgaaaca aatccattga agttcctccc
    42721 cattttaggt tggaaaacag cgattaaact gttttcatat accctaaaag gataccacct
    42781 aaaatgacta aaaggcaacc aacaataaca taaatgagct cttttttcgt ttttctttcg
    42841 ccaaggatga aaatcccacc taaagtagag ataatgatcc ccatttgtga aagtgagaag
    42901 ccaacagcta aaccaagtga tttgactgtt agaagcatgc agatgtttcc taagccccat
    42961 aataatccac agatcatgtt tttccacaca aactgatcga ctttaacttt acggaaagca
    43021 aagaagcttg cgcctaagat cattccgata ctttgaggta agataattga catcgcatcc
    43081 aaattcgccc agttgacgat gatggtatat acaccataac caatcgtaga gaagatcaat
    43141 gctctaaaac ccttaccaaa atcaagcatt ttattatctg tatctatttt tccttcgtca
    43201 tcttgtcggg cagtcaaata cgcaccgctt acaagaagag ctaaagcaat aataccgtat
    43261 acataatctt tcgtttgtgt ccattcatgg aagaaaacgg ctcccgcaat cgtattcaaa
    43321 attaattgaa aaccagtaga caatggtagc cctacagaaa cacccatgta cttcattcca
    43381 tggaactgtc cattttgtcc aacactccaa gctaagccgg ataaaaatcc gatcaagaaa
    43441 atccaagggg tgattacagg actaacaacg aaaaagacaa ctaaagaaaa cagaaatgca
    43501 ccgatcgtca ttcctagtgt ttgttgattg gcatcgccac caattttacc acttactaaa
    43561 ccaatactac cccatgccaa catagggact aatgctatta aaatttccat aaccaatctc
    43621 cttttatttc acatgataag aaacttatca gcgaaaacgt taacaacaca agcacttttt
    43681 ttcgtttcta acgattttct aacgtttttc attataaaaa tttaagattg ggaaaaataa
    43741 agtcattgtt gtcccttttc cttcagttga ttcagccgtc aaatggatac ctaatttttc
    43801 ggccagattt tttgctaaat aaagtcctaa tcctgttgaa tgtgttttgc taagtcgacc
    43861 atttctccca gtaaatccct tatcaaagat ccgtttcaag tcttccgctg ggataccgat
    43921 ccctgaatct ttaactgaaa ggctgactcc ttgcgcttcc ttttctatgc tcacaatgat
    43981 gtttccgtga tccggcgtat atttgatggc attactaagc aattggttga agataaaagc
    44041 gatccattta gcatcggtca aaacagtatg atcctcacca acgatctcgt agcgaatatt
    44101 tttttggata aaataatttg cctgactgcg tatcacaggc tgaatgattc cttttagtcc
    44161 atattcttgg atgaggtagt cccgcgagaa actgtcgagt cttgcataat acaaaacttg
    44221 ttctacgtat tcatcgattt ttgataattc attttctagt aatgtatact tttgatcgtc
    44281 aatatcgaac tcgatcgaac gaactagcaa tttagaagcg gaaagtggca ctttgatttc
    44341 atggacccat gagtcaatat aatctttttg gtcttgttga ttactgattg ctgattgcat
    44401 tacttcttgg tgttcacgga tcagctgatt gatatattct tctaccagtt tttcttcttc
    44461 tgtcctccca ccactcaaat aattttgcaa aggggaagtt ttttgtatgt cggctaattt
    44521 tccccaccat cttctcttgc tcaagtaacc acccagcaga taaaaaatca gaaagacgcc
    44581 ctcaatcaaa gctaaataag caattgtgct caaatcaact ttgatgtttg gcgccagcca
    44641 catcacaaag atcgtcaatg caataaaaaa cagccatccc aagatcaatg tccactgatc
    44701 tttcaaatat ttaaaaaaat tcatcttaac cgctcatcct ttcagggaac gatatatccc
    44761 tgtcctactt ttgtttcgat atatccttgg atacctgcct gttcgatttt tcggcgtaac
    44821 cgattgatgt tgactgtcaa tgtattgtca tctacaaatc gttcgtcatc ccacagtgct
    44881 cgcagcaatt tttcccgact taagatcttt ccattgtgct tcatcaaaat aaataataga
    44941 cgatattcat ttttgctcaa atcaatgatc tctccgttga tttccatact tccgttgtca
    45001 acattcaacg taatgccatt gtggctcatc atttcactag ataattctgt gtagttgtaa
    45061 gaacggcgaa gtaatgcatt gatttttgca atcagtacat cgatttggaa atgttttgtc
    45121 acaaaatcat ctgcccccat gttcatagcc atgatcatat ccatattcgt attcctgctg
    45181 gagataaaaa tgatcggtac tttcgatact tcacgtattt tttgacacca ataatagcca
    45241 tccaaaacgg gaagattaat atctagtaat acaagctgtg gttcttctct ttcaaagtct
    45301 gagaacactt gattgaaatc ggtcgttcca aaggtttcaa attgccattt ctcaagttcc
    45361 tccatgatca attgtcgaat gatctcttcg tcttcgacta ccatgatttt tgccataaaa
    45421 aatccctcca tctttcttct tgctactaca atatcaaaga agcccttgtg aaacaagttg
    45481 gcttacctgg aaaatgtcat tttagcaaac gttaaaaaaa actggaaata tgcgatttaa
    45541 tcgcgtttgt ctcttccaag cagcttaaat ttattatgtt attcttaata attggtctat
    45601 tatctgttat ttttctttcg ttgtttttgt cttttaaatt ttcgataaca ccaataaccg
    45661 aaaagcgtta gcactagcaa tatacctaga aggagtataa gtaatcgata aagatggtat
    45721 ttttttgtct gttctatctt tgaagagact tcttctgtaa agggaactcg tcttcctgtc
    45781 actagtaatc ggtgagtgtt cacggtgtaa ggtgtacaag tgataagtgt gactagatcc
    45841 tgcttttctc taattgctaa tgtatttgtt tttgttggaa ggacaacaat tttctcaacg
    45901 acttcataag cgagcatccg accatacact tcaagataga atcggtcatc ttgctccatt
    45961 tcttcaagat ccgtaaagag ttttttctca gcaataccgc tatgggcggt aatcacggaa
    46021 tgtgtactct ctcctccagt tggaaaagag gtgccttgca ggactgtcgc tcctctatct
    46081 aacaaaagat cattcgtttc atcaaataaa ggcaagctaa cattgatttt cggaatatag
    46141 atggctccga tcatatgttg ttcgtaatat tcttttcctg gattttggac atttctaacc
    46201 gcttgctcaa aaggatcttt gacttgtccc attcctggga ttactgatgt atgagcaagc
    46261 gtctgatttt tcttttcatg cactgctaat tgttgctcat gtttttcttc tttatcgcgt
    46321 tcaagctgct tttgataatt ctcgatcctt ctttgatcca aataattgct gagagcatct
    46381 gctaaaaatg gatatgtaaa aactgcagct ccggcaagaa acatcactgc cataaataat
    46441 tgtaataaaa gactgatttg ggtttgaggt ctattaccta ttttcttttt cattttcttc
    46501 accttcttat ttaaaaaaac tgcagcaacc tttataaaac cgattgctgc agcttatttt
    46561 tgttgcttat acttcagact tcattttaga tttacggtac cagctgtacg ctccaatcat
    46621 caaacttatt ccgattagta agaatgccaa gagcccgttc ccaccagtag caggaagtga
    46681 cccgcgtttc atattcggta ctctttctgg ccctggattt tctactccta cgatctgaat
    46741 ttcttcatta tagcttccat aagagatgat aaattcagta aaggctgcct cacctgttgg
    46801 aagtacataa ccttctggcg ccatggtctc tttcatttga taggttccat aatctaaccc
    46861 ttgaacctca aagtatccat ttccatctgc gacaaattct gttgcttgtc cgcttgttgc
    46921 ccactcaatt ctatctgctc ctgattcata aacagctaaa ggcgactccc ctttataaaa
    46981 tattgcgtat tcagaacggt cttcattcca caattcgaat cttgcatctt taagcaattc
    47041 actgcttgat gcatcatatt tttcaaactt tcttccacct gttgtgactt tctctggcgg
    47101 ctctactcct tcttcataat caaattcatc atttcgtccg atttcaataa ccgctgtgtt
    47161 gttatggaaa tcatccgctt ctagttctgt ggaaaccttc aaattatacg tgatcgttaa
    47221 atctcctcca cgtgcatctg ctaatctacc taaagtatct actttggcta ctccattttc
    47281 ttctgtcaaa atcgtcacga caaacccgtt gcttactact tctactatat aatcttgtcc
    47341 ttttgttaaa atcgtctcgc cggctcgcac ttctatttcc tgatcataat aagttagacc
    47401 aactgtaggc gtatctgtga ttttgtaatt tttgataaca gcgcttccgt tttcaaggac
    47461 agaatcgatg aaataaggaa taggtgctgt gatcgtgtaa gaagccgttt ttcctctttc
    47521 tagatcaata taagaaatcg tttcttgttc tccagcttca ttaataatcg ttacttgtct
    47581 aaggtcgctt tcctctacat tcatctgttt atctgcttct ctgatttcgt ttttcggata
    47641 caaatgcaca tctgtattgt atgtgtcttt ccatgtagaa ccatcccaca cgccatccac
    47701 catatcaggc atcatcactg gcatcataac aaccattgga gacgcgattc tggaaatatg
    47761 agctggcgaa taagtttcta agaaaagata gactttatct ctagaagaag gtgcttcttc
    47821 cgtcacttgg acggtagaga ataccgcttc tccagcttca ttcgttgttt gtttgtcaat
    47881 acttgtcagt cgatatttta aaaaccaact ttctgtgtgc cgtttttgaa tccattcaat
    47941 cgcttcggca gaattaagac cgtcttctcg ttccggtgtt aaaggattat ctcgaatcaa
    48001 atcgtaatat acttccgtta cgtcaaacaa atcaaaatcg actcctggaa tattttcttc
    48061 accaaaatcc gtagaaacca atccgctatt cggtcgttct tccggaacag atgagaaacc
    48121 ttttttgtgt aatgtaatcg tcacttcact tggtttttgt gcgacttctt cggctttgac
    48181 tagttttccg ttcccgaaaa tccctattcc cgcagtaacg agtaacagta cactcattcc
    48241 cgtgacaatc atttttttat tcattttttt ctccccgttc tttttttatt aataaaacag
    48301 cacctaacaa taaaagaatt cctagtaaag aaatcaacgt tcttgcttca ttcgtttgcg
    48361 ggaatacgga ccattttcct gattgacctg gattgcttgg accactcggc tctttcggtt
    48421 tgtccgttgt ttcttttacg gtctcgttat aaacacgtgg tgttgcggat tcataggcag
    48481 agactggaac tttcccggat tctaattcag ccatctcctg tccgttaact actacatatt
    48541 gtggttggcc ttttgcatcc acccaaaatt cagggatgat gacctcgatt ctcttacttg
    48601 cttcatccat ctcgtaacct tccacacctt ttaattcttc aaaataatag gtgccagaag
    48661 gcaagaaacg ttgtcctgtc gtaacaaggc cctctttatc cgaaatgaat ttgcttacat
    48721 ttttgtcatt caaaggatca ttgtctgctg gtttgatcca ttgatttttc aagtcattag
    48781 caggagccat atctaaataa tatttccccc cttctatcat ctgatataga gcaaaaacag
    48841 caccttctaa tggttttcct ttttcagttg ttcctctttg acgtccatac ttgaagaagt
    48901 atggatctct tagataaccg acatttttag gatagatatg aatctcttgc agttcctcgg
    48961 ttgattctgt cgggtgataa attggcaaaa tagcagcaat tgggatagct gtctgatcga
    49021 tatcaacatc caatccaact tctggatcaa tcgattcttc gaaaatcaaa taaactgcat
    49081 cccgatcttg gtttttctta ggtgctttga tacgtgcaat cccgtctcct aatccgccaa
    49141 ccgtatcttt cccagttgtt accgaagtcc ctatttggac aagcctttct tctgtgatta
    49201 gctgacggat attgtttctg ttcgtttcag cgatttgctg gacgaattct ttaggagtca
    49261 tgttttcagc agcttttttt tcatgataat aatcggtcat gtcgtaaatg gcaaatgtcg
    49321 ctccatttag tggcaccgtc tgtgaaatga tatctgctgt ttcccctgct tcctcagaga
    49381 tagccaagcc atcattttca tggtattcaa attgatcgtt ataatcgata tctcgaatca
    49441 tccgtttatg caacacgaat tccacgacat cttcccctga ttcttctgcc gacatcgcat
    49501 gtggaagaag gactgctgca agcggtagta aaacaagtaa ttgaagcatc aaacacaaca
    49561 gctttttcat taccttgctc cctccttttt agattgtctg acaaacaaac cataggctgc
    49621 tcctgcaagc gccatgaaga atgcagcaag caggatatag aataatcgtc cggatcctcc
    49681 tgtagcaggc aacggatttt caggatcgtt cccgacacta agctgtaatc tgattcgatt
    49741 tgtctccaaa agttcatatt ctaactcttg ctgtagatca ggcgtctctg cttcttcgtt
    49801 tggataatac gaaatctcaa cttttagtgt acccgcttct tttcctggaa ctacccgatc
    49861 attttctatg tcaggttctt ctcgataact aatttctaaa ataaagtaac ctggaagttt
    49921 tctatagctg tcaggtgact tcacttcctt gatccgatag accccttcca acaagtctga
    49981 agcattgaat agggaagaat cgtttggatc tggtgccaag cctccctcga tgatttcata
    50041 ctctcctgca ctattttgtt tctcaattga aaattctgcg ccttcaagac gtcggctatt
    50101 catactgtca attttcaaaa gttccaaatc gattggcgct agttcatctt ctaaggttac
    50161 ttgtagtaca ccatcgatga tttcatagcc ttccggcaag ggagcagttt cgtcttcgat
    50221 gaccgtatct tcaatgataa acttaccttc ttcattcagt gcaaactgga acacttgatc
    50281 agatagccga tacccatttg gtgcgacaga ttccgagacc tcatattcac caatagacag
    50341 atgcagaggt tggtcgtaat ttaaatcgac atacttttct actcctacgg cacgagaata
    50401 gaagtatccg atcccaggca aacgtttcga ttcttctggt tctaacggat taccagtgta
    50461 tgctcgatac agcgggaagg aagaatcccc cactttcctg atatcgaaac ctgcaccatc
    50521 taatgtatgg ccttcatggt ctaattttct aactcggatc tcccgataat caaacgtatt
    50581 gtaagcagtg aatgctaccc ataaacgatc ctcttcatct gttgtatacg tgagatattg
    50641 ccaattttct tcacctgttt gcttgagttg gatagtccct atagcattat cgtaaagtcg
    50701 caagatccat gtgtcatttt catcaggttc tttgaaacct tctgggttct ggctctcaat
    50761 gattttgtat tctgcatagt tggtagtttc ttcactatcc ttataaacaa atgaatctgg
    50821 ctctccgatt ggatgaataa ctttctcacc atccacgatt acatatttcc cgtcttcatc
    50881 tactgaatag aacgtgagtc tccctttctc ttcctctact tcgttcacac cagaaagccc
    50941 tttcgctact ggatatccga tttccagtcc gttaccatct gtttcataaa gttcaaactc
    51001 tgcgccattg attggcatat tactgccttc aattagttta ttgatattca tgtggattgg
    51061 cttgaattca tttcgcattt ccagtttgac atacaactca tcatactctg tgccttctat
    51121 gttaggctct tgtacaacag aaagcttgtg atattctcct tcataatagg aattcggatc
    51181 tttaccttcc ggtccgccga ctctcgaacc agatgtatat tcatctacaa gctcgtagtg
    51241 acctaacatc tcatcttcgt tggctgcata ttttttgatt tggaattgat acgtctcatc
    51301 tactaactga taacgtggtg gtccagcgat ctctctgatc caaaatatgt cgctgactgc
    51361 tccagtttca tctcctaaaa tcggcatctt cgtttcaaag atcgctattc cgtccgtggt
    51421 agttttactt gtttcgattg gttcggcttc cccactctct ggattttggt catataatgc
    51481 aaattcgact ccagccaacg gctcgccatt ttgacccttc ttgctgactt ggaaattacg
    51541 gcctttatgt tcgtaacgaa tggaagaaga tgggaaatac atccgtccag tagtgatatc
    51601 attctcataa tcctccaggt tctgaatata ggccatattg ttcactgtgc aaaaatttcc
    51661 gtcttgagct cccgcatcaa gttttacttg gtacttgatc ctgaccattt ccccttcacc
    51721 taaagaaatc ggagaaaatt tataagcttg gttttcccgt ccgacatacg ttgtcggagt
    51781 cgccactact tctggtgggg cttgtccatg acgttcttca tcccatattt ctggctcttg
    51841 gtacttcatg ccgttttctc ctggatctag atggtaaaac tgaatatctg ccgatctgcc
    51901 agctccaaat ggtgagacat taaaaccagt ttcatcataa agtgtggctt gctggatcgt
    51961 tctcttgaag gtagaagaga tatcctccaa ggctgtaaca atgtcattct tgttgttcgc
    52021 attgtaatat cgcggtgtta cgccatcttc ttctaaatca gcaatatttt tcaatacatt
    52081 tcgtccaata gcatctagtc gttgtctccc gctaacgttt ccatcgattc ctaaaccaat
    52141 ggagaagata cttggcgggt tctcttgata cttgtttttc agccaatgat gataagcaac
    52201 tgtgttgatc gcagatgact tttcttgcga agtaggactg ccattcgtat tgttattaga
    52261 agaaccatca ccaacccagt gagtcgcttt attatctcct tcttttacct ctccataacg
    52321 atagtgtaca tttacttcat cttctggacg gttaaagtca gatgcatagg taactggatg
    52381 agtaaaccct gcattagaag tattcccaga aaactcctca aaatttctga acaaaacgtc
    52441 attatcttca gcaatcttat cttccatcac agtccagttg ctccaagctc cattagtgct
    52501 cgtacgataa cttgactgaa tcggtgcata actgaaggtc ggtgtcccat ctcccacaac
    52561 gattaggatt ttttctggat ttctattttc tccgccattg tctttataca atgtttcata
    52621 gccattttta agccctagtg taagcggagt tcctccgatt ggtgtacgat ttaatgctgt
    52681 attctcactt cccgtcaaac gattgatatc atttgtcaat ggaaagatgt ccgaactgat
    52741 ttgctcatgg ttattgatag ctgtactatt atggtaaaaa ttcaccatac cgattcgaat
    52801 atccagactc gggtcagaga gcagatcttt actcatttcg tttactgcct caatcaacgc
    52861 agcattttta gtttgactat tggtcccagc ggttaattca ctcatactcg ctgacttatc
    52921 tagaacaaaa acgatatcga ttggactgat ttcctttttc tcgcccccga tgacatctag
    52981 atagacattg taaagggttg ttgcatcttc aggatgtaga tcagatgctg tgctgcttgg
    53041 cagaacaggt tctacccatt ttttgatgta cgccccgtta taatccaagt aactgttttc
    53101 aaaattccgt tcatttcccc aaagctgaac ggtttgagct ggaacgtttc tatcttcttc
    53161 gcctgcagtt gatccattat tagtagcata gtcgtaattt cgccagttcg ctcccccgtt
    53221 tgtataatca ctatcatcga tcaagttctc tccgtcatat ggcgagcctt tgtctccttg
    53281 atagcgttca ttgctttgtg tccaatttcc taacactttt gtatagtcat tcgttgtaaa
    53341 cttcgggtag cgatgctgtt cgttttcaac ttctccgcta ggatagatat attcataggg
    53401 gtcttgcgta gctgttggcc caagacgctc caaaataccg ttgaataact ggcgttgaat
    53461 gcgctggagt gcttgaccgc tgggtacgat tggttcgtcc agcttttcca tctcattttc
    53521 ttcgctacgt tgaatttcag cggaatcttc tgtatcagtt tcattttctt gtaattcttg
    53581 gttatctatt ctttcatcaa ccaccacttg aagaggtagt tacccttcgt tcacatcggg
    53641 gatcttttct gtttcaaaaa tcaatgtgcc ggtttctgct tcttttgaat aaacttttcc
    53701 gacataccat tcttgaccat cgatcgtctc gacttttagg tcttctccat cttcatcata
    53761 ggaggataga cctgttccac gaacgttttt tacggtacca atcccggcgg ctgccgcatc
    53821 gattgctaaa cgtattctac cttcattggc actttccttt ttttcaaaag ccagatgcca
    53881 ttcaagggtt tcttcttttt caatatactt cccttcaatt tttgcatagt cctcatctaa
    53941 gaatacctca tcctcctttg tttctgttga ctcagcagct acatccaggg tcgtcatatg
    54001 atagactgac tggaaatatg gtccaaatag caaactgaca gttactaact gcaaaagctt
    54061 caattttaac cctttcacat ccttatactc ctttcaaaaa tatttgtctt ctttcacaac
    54121 aatcaacaaa acaatgccca tctaattata atttaattta attttaattt taaatcaaat
    54181 gatatccttt ttagttaaaa aacaccaatt atacaacatt tattattaaa attaaattat
    54241 aaaattttat aacgttctca tttaacaaaa cttaagttat taagacaaat gaataaacaa
    54301 ctacgctttc ctcgaaacaa aaaaataaag cacaaatgcg catttacgca ctcatgcttt
    54361 attcgtacaa ttaattcaga taacggcagc gcataggatg ccaataacac ttcctaaaat
    54421 agctcctcct aaaatgtcag taggataatg taaaagtaaa aaaatcctcg aataagcgat
    54481 caaacaagca aataaaaagg ctaatattcc tgcaactgga cttaattcga acaacgcagc
    54541 tactgataaa aaggctagcg ctgtatgtcc tgaaggaaaa gaagaagtct tcggtttact
    54601 tgtgtaaagg acaagccgac gatttttttc ataaggtctt ttccgtcttg tcgttttttt
    54661 gataaaaagc aaaccaacaa aaaaatagat caaactgccg ataagaatgg ctagaccata
    54721 ttctctagta tcaggtatca agactaaaac agcagcaagg agcaaccaaa taaaaccacc
    54781 attagagata ttactgataa atttcaaaaa attgattttt cgactatccg ttttccgttg
    54841 cttggcgaac ttgttgataa tactatcatc gattttagca atcaaacgca acatgttatt
    54901 cctccttttt aagaaccttt tcttgcgtac gatggataaa aagcaggact tgaaaataaa
    54961 acaagaaact gaaacctaat aaagcaaaat agattggtcc tgcaaaccag aatgcgacaa
    55021 gaaggatcaa cgtgcctgcc aaatagaaaa gcgcgctttt gaaatcatta aaaaacaaga
    55081 aaaatgcagt aatccaagaa gcttcttttt gttccagttg aagcaagatt ttgtaactaa
    55141 agagaagcgc gcttaataga agcaaaaaca acgtagtata catcaatgta tagccaattc
    55201 ccccacccca atagcggaaa atctgataat cgatcaatag aagaagaaaa taaacacttg
    55261 ctaaactatc ttgctttcca taaagagaga agttctcacg aaactttttc cagaatagtg
    55321 aaccgagctt tttctcttca tttttttgta atgataaatc atatacagaa ctgttcgact
    55381 tgcagttcct aatgtgaaaa caggcaagac gcacaggaac catagtagct gtaaatggat
    55441 aagacgtgga atccattcaa tgatttctaa gaaataatca ttggcttttt taccaatcga
    55501 tcgattcatc atccttttga acctcctgtc aaatccatcc cttcaatgaa ataacgttgt
    55561 gcaaaaaaga acagcaaaat cgtcgggatc aagacaagtg ttgctcctgc catcagataa
    55621 ttccattgtg tcgttgcttg gttctgaaaa acttgcaagc caatctgtaa cgtgtatttt
    55681 tcttgatctt ggatatataa cagaggacct aagaaatcat tccaagctgc gttgaaggaa
    55741 ttgatcgcaa tggtaatcaa ggcaggtttc gtcaatggaa gcatcaaatg actccaaata
    55801 tacaaatgat tcgctccgtc gatttcagca gcttcgatca attcattatt gatcgataag
    55861 taaaattgac gcatcagaaa tatattgaac gcattgccga agaacgatgg aacgatcagg
    55921 ggcaaataag tacctaccca gccgatcttg gagaataata catattgagg gatcatcgtt
    55981 acaaaccccg ggatcatcat cgtagaaaga accaaagcaa acatcagctt tttcccagga
    56041 aaatcaagct tcgcaaatcc ataagcaata aaagaatttg aaaggacatt accgatcact
    56101 actagcacag tgatgaataa tgtatttctt gcatagcgcc aaaaaggaaa ggcggcaatc
    56161 gtatccagat agttttccca tacgattttt tctgggaaaa aggtcggagg ataacgaacg
    56221 atttcttcca ttggtttcaa tgaagtaaac accatccaaa gcaatggaat gatcaaaatg
    56281 attcctatta ctgtcatgag gacataactt agtatttttt tgtttgccga ttcacactgg
    56341 tctttttgaa ataatccatc atttgccgtc acctccagaa taatagaccc acttaggtgc
    56401 aagcttcaaa ttgacaaacg tcagaaccaa cacgatcaga aacaggatca ttgacatagc
    56461 catcgcataa cccatatcaa aagctacgaa cgccttgttc cacatatgca gattataaaa
    56521 aagcaaagaa tttgcaggtc cgcctgttcc atttttcgtc atgacataag cttcttgaaa
    56581 aatttggaaa gaaccgatcg tagaggtgat cacatcgtaa aaaataatag gagtgattaa
    56641 tggcaaagta atacttttga actttcccca aacgcctgct ccatcgattt ctgctgattc
    56701 atataaagat ctaggaacat tttgtaatgt agctaagtaa agtagcattg ctcctccaga
    56761 actccatatt ttcatcaaaa tcaacgcagg tttcgtccat tgtggatcaa acagccaagc
    56821 tggtccaggg atatggaacc agcccagaaa tgtgttgatc aatcctgcag atggagaaag
    56881 cagctgcatc cataagatgt agactgctac tcctgataag actgccggta aatagaaagc
    56941 tgtacggaat acactgattc ctttgatttt ttgattgagt actgtggcta aaaacacact
    57001 gaaaatcgtc gtcagcggta cattgaagat gacgaaataa acggtgttgt ataacgattt
    57061 ccaaaataaa tcatcgtgaa cgaacattct tttgaaattt gctagaccaa tgaaattcat
    57121 cttggatgtc aaattataat cggtgaaact accaataact gaaaaaagca taggccctag
    57181 cataaatacc aagaacccga taataaaagg cgagataaat aaataggccc atcttgcttc
    57241 ttttttcttt cggatagata aacgcttcaa attttcttgc ctcctctgta cctagacagt
    57301 caaaaatgga ttattgattt tgcttgacta agttttctac tgctttttgt gcatctgcca
    57361 atccttcttt tggtgatttt gttcctaaaa gaatctggtc aatctgttca ttcactaatg
    57421 tcgtgtaatc cggagcatct aaaggaactg gtgtgatcac cgtattttta aagttttcgt
    57481 ccgccatttg atagatcatt tttcctttat ccgttaggtt gtcattattc actaaattct
    57541 cattggctgt cttgctcgcc atgatatcaa aacttttttc gccgaatttt tcttgtactt
    57601 ctggagaaga aagatatttg atgaattcat aagaggcctc aggatctttt gctccgtatg
    57661 gtacttccaa gacgaatccg ccaccccacg accaatggcc tgaaccttct tctttttctg
    57721 ggagctgcat cacgccaaaa tcaaaatcat ccggtgcatt ttccatcaaa ctagagtaat
    57781 aattgatatt ttgggcacgc atggctacta atccggaaat aaatggatca gatacaccag
    57841 acccaaattc ggcttctaat ttattgatcg tatttttccc ataataatct tgccagtctt
    57901 ggatccactc caaggcttcc actttatttt tagtatcaat cttgactttt tcatctttgt
    57961 caaaccaact agtcccgtta tctgcgttta gcgcccatac atcagcccct aaattccata
    58021 atggataaaa accgatgcgt gtaaattctt ttccttcttt tttatctagt ttacgtgctg
    58081 tttcttctaa ttcatcccaa gttgtcggta gatcttgttc actgattcca gcttctttaa
    58141 atatcttttt attataaaaa atgacttgtg tatctgtatt gaatgggatc gcataaggat
    58201 caccttcata ttctactgta tcccaaagct gcggataaaa atccttagaa gtgtcttttt
    58261 ctacatattt ggagagatta gttgcttgct gtgcttctgc tctttgggca acggaattta
    58321 tgtcctgtac aataacatcg ggtgcatttc cagctgttat tgcagataaa gatttagtcc
    58381 agatgtcacc ccatggctga tacttataat ttacatggat ctcgttttgt gattgattga
    58441 aatcctcaat gatttcttcg atagcttctt ttcgtgcacc ggatccccaa aaggaccaga
    58501 aatcaatatt ttttcttcca tcacttgttt tttcagaaga atttgctgca cttgaactgg
    58561 catcgcttgc attacttgat ccgcatcctc ctaataaaaa caatgctgaa cttgccagca
    58621 accctgtaaa caataatttt ttcatatttt acctccaaat tttaatgtaa ccgatgccgg
    58681 ttttaatata acgatatcat tttgttctga aaataacaat ttataaggta tgattctaaa
    58741 aaaattatta atgataccaa tactttatat aattcgcgag cgctttcttt gaatttttta
    58801 atctttcgta gcagaatgag aatgtattaa aaatagatta ggaggaattt tgatgtttag
    58861 acaccagaaa gaattgcagt ttgaagcaaa accagatcgt ccgaatccat taatcgcgaa
    58921 atatctgcag gagctgatcg gcggccaata tggcgagatg agtgtagcta tgcagtacct
    58981 gttccaaggc tggagctgcc gaggagaaga aaaatataaa gatatgctga tggacattgc
    59041 aacagaagaa atcggccacg tggaaatgtt ggcgacaatg gttgctcgtt tattagaggg
    59101 tgcgcctgtt gaagatcaag aagcagcgat gcaaaaagat ccaacaatcg gtgccattat
    59161 ctctggaatg aatccacagc acgcgatcgt agctggctta ggacctcgtc ctgctgacag
    59221 cgtgggcaat ccatggagcg gtgcctatat cattgcgagt ggcaacttgt tagcagactt
    59281 tagagctaat ttaaatgccg aatcacaagg aagacttcaa gtagcacgta tttacgaaat
    59341 gatcgatgat cgtggcgtaa aagatatgct gtctgttctc cttgcaagag atagctatca
    59401 tcaaaatcta tgggaatgtg cagtgaaaga attagaagaa aaagaaggtt caatcttagt
    59461 cccaagtact ttcccgcgtg acaaagaacg aaaagacatt gcttatgact tctataactt
    59521 ctcagaggga gaccaaagta gtgaaggttc ttgggctagt ggcaaagcac tggatggcga
    59581 aggcgaatat caatggttga aagaacctaa gatcgaaggc gctgcaccaa aattagaacc
    59641 agtaacacca aaaatgtatg ggacacctcc attcaagaaa taaatatcta cccgtagaaa
    59701 cacccgtttt ttgttccaca cgctttgtga gcaaaactga ctaaagtcat taatcataac
    59761 cacccttcaa aagggtggtt ttctctagag ctataagctc aaatcaccgg ccagcgccta
    59821 aagacgctgg ctttcatatt ttatgttcaa gccatattgc ctttgccagt tttgcccacc
    59881 tcttaaaagg tttatgtact actccttagc ccttaaaagg atcgtcttac tctcgtacac
    59941 tcagcttatc taacgcacta tcatgttttt cttgttcttg aatgtacttt ttgatggtcg
    60001 cttcattttg tcctactttt gtcacatagt atccttccac ccaaaagtgt cgatacccaa
    60061 atttatattt tagatttgca caatctcttg atgatctcct gaatattggc tcgatattga
    60121 ttataaatta tttttcttct atactttggt gtgaatacaa tgtggtattt acacatctac
    60181 tttgtacggg ctaaactatt tccctttatt gctccacccg catagcaagt ggttttgtat
    60241 ttcaatcgct cggcgcgctc aattaggtct aaagacaata aatctaaaaa cccgctgctt
    60301 cactgacaga aacaacgggt tttcagaaaa aaatgaaatg aaaaagatct cttacggcaa
    60361 tctattttat ttttttgatt ttttataaat ggaaaatgct tcaagaagtc caagaaataa
    60421 agccgtataa aaacaagaac ctatgaaatc tttatttatt agatattcaa cagaaatccc
    60481 gataacacta gccaacaata tatttatcag aagtgtaaaa taaggatgct ttttatagaa
    60541 tagaactagt gttgttttca tagactctcc tttcttcata tttccaatta atcacaaatt
    60601 cacggcatta ctagaaaaat taaaggggcg cagaagtgga aaaatactct tcgactttcc
    60661 ctacaaacca ggccatcttg tgtttaataa ctataccaaa tctaaggtac ctctaaatct
    60721 aaattccatt tgtggcggtg acctccaatt tttaactctt ctatctccca tccagtatac
    60781 ataaccttga tacttcgacc acttatatat cgaacacaca aatacctatc cattgcagga
    60841 gtactaccta acttagttgc tacttgcatc taaataacag ctgaaaagat aggacttact
    60901 cttagtaaag tatctgaact attaattttt aaactatcat ttttatcctc aactacatgc
    60961 acatctgttg caacttcact ttctgtctca tccagataaa tgattaagga atcgggaatt
    61021 gcaatatctt cagctgacgc tggaatagtt gagctaaatt agtcatcata tctttattat
    61081 aaaataac
//
LOCUS       DAZKLA010000002        54054 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000002 DAZKLA010000000
VERSION     DAZKLA010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.002"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..1666
                     /locus_tag="ACG85N_000055"
     CDS             35..1666
                     /locus_tag="ACG85N_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFQ0222865.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(1728..2369)
                     /locus_tag="ACG85N_000056"
     CDS             complement(1728..2369)
                     /locus_tag="ACG85N_000056"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0222866.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    2555..2631
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            2651..2959
                     /gene="rplU"
                     /locus_tag="ACG85N_000057"
     CDS             2651..2959
                     /gene="rplU"
                     /locus_tag="ACG85N_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFQ0222867.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            2986..3330
                     /locus_tag="ACG85N_000058"
     CDS             2986..3330
                     /locus_tag="ACG85N_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFQ0222868.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            3343..3636
                     /gene="rpmA"
                     /locus_tag="ACG85N_000059"
     CDS             3343..3636
                     /gene="rpmA"
                     /locus_tag="ACG85N_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFQ0222869.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4070..6877
                     /locus_tag="ACG85N_000060"
     CDS             4070..6877
                     /locus_tag="ACG85N_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0222870.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            7068..7559
                     /locus_tag="ACG85N_000061"
     CDS             7068..7559
                     /locus_tag="ACG85N_000061"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0222871.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            7662..8651
                     /locus_tag="ACG85N_000062"
     CDS             7662..8651
                     /locus_tag="ACG85N_000062"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0222872.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            8684..9487
                     /locus_tag="ACG85N_000063"
     CDS             8684..9487
                     /locus_tag="ACG85N_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFQ0222873.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            9508..10422
                     /locus_tag="ACG85N_000064"
     CDS             9508..10422
                     /locus_tag="ACG85N_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0222874.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            10505..10879
                     /locus_tag="ACG85N_000065"
     CDS             10505..10879
                     /locus_tag="ACG85N_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFQ0222875.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11018..11263
                     /locus_tag="ACG85N_000066"
     CDS             11018..11263
                     /locus_tag="ACG85N_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0222876.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            11435..12499
                     /locus_tag="ACG85N_000067"
     CDS             11435..12499
                     /locus_tag="ACG85N_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFQ0222877.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            12745..13170
                     /locus_tag="ACG85N_000068"
     CDS             12745..13170
                     /locus_tag="ACG85N_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0222878.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            13163..13612
                     /gene="nusB"
                     /locus_tag="ACG85N_000069"
     CDS             13163..13612
                     /gene="nusB"
                     /locus_tag="ACG85N_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFQ0222879.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            13709..14563
                     /locus_tag="ACG85N_000070"
     CDS             13709..14563
                     /locus_tag="ACG85N_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFQ0222880.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            14564..15901
                     /gene="xseA"
                     /locus_tag="ACG85N_000071"
     CDS             14564..15901
                     /gene="xseA"
                     /locus_tag="ACG85N_000071"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFQ0222881.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            15906..16130
                     /locus_tag="ACG85N_000072"
     CDS             15906..16130
                     /locus_tag="ACG85N_000072"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFQ0222882.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            16132..17022
                     /locus_tag="ACG85N_000073"
     CDS             16132..17022
                     /locus_tag="ACG85N_000073"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0222883.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            17031..17849
                     /locus_tag="ACG85N_000074"
     CDS             17031..17849
                     /locus_tag="ACG85N_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFQ0222884.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            17956..18405
                     /locus_tag="ACG85N_000075"
     CDS             17956..18405
                     /locus_tag="ACG85N_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0222885.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            18423..20105
                     /gene="recN"
                     /locus_tag="ACG85N_000076"
     CDS             18423..20105
                     /gene="recN"
                     /locus_tag="ACG85N_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFQ0222886.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(20160..20276)
                     /locus_tag="ACG85N_000077"
     CDS             complement(20160..20276)
                     /locus_tag="ACG85N_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFQ0222887.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(20349..21305)
                     /locus_tag="ACG85N_000078"
     CDS             complement(20349..21305)
                     /locus_tag="ACG85N_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0222888.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21476..21844
                     /locus_tag="ACG85N_000079"
     CDS             21476..21844
                     /locus_tag="ACG85N_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFQ0222889.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            22014..22445
                     /gene="mraZ"
                     /locus_tag="ACG85N_000080"
     CDS             22014..22445
                     /gene="mraZ"
                     /locus_tag="ACG85N_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFQ0222890.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            22466..23425
                     /gene="rsmH"
                     /locus_tag="ACG85N_000081"
     CDS             22466..23425
                     /gene="rsmH"
                     /locus_tag="ACG85N_000081"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFQ0222891.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            23430..23831
                     /gene="ftsL"
                     /locus_tag="ACG85N_000082"
     CDS             23430..23831
                     /gene="ftsL"
                     /locus_tag="ACG85N_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFQ0222892.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            23832..26024
                     /locus_tag="ACG85N_000083"
     CDS             23832..26024
                     /locus_tag="ACG85N_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0222893.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            26050..27012
                     /gene="mraY"
                     /locus_tag="ACG85N_000084"
     CDS             26050..27012
                     /gene="mraY"
                     /locus_tag="ACG85N_000084"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFQ0222894.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            27026..28387
                     /gene="murD"
                     /locus_tag="ACG85N_000085"
     CDS             27026..28387
                     /gene="murD"
                     /locus_tag="ACG85N_000085"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFQ0222895.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            28413..29501
                     /gene="murG"
                     /locus_tag="ACG85N_000086"
     CDS             28413..29501
                     /gene="murG"
                     /locus_tag="ACG85N_000086"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0222896.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            29516..30736
                     /locus_tag="ACG85N_000087"
     CDS             29516..30736
                     /locus_tag="ACG85N_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFQ0222897.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            30892..32217
                     /gene="ftsA"
                     /locus_tag="ACG85N_000088"
     CDS             30892..32217
                     /gene="ftsA"
                     /locus_tag="ACG85N_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFQ0222898.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            32240..33481
                     /gene="ftsZ"
                     /locus_tag="ACG85N_000089"
     CDS             32240..33481
                     /gene="ftsZ"
                     /locus_tag="ACG85N_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFQ0222899.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            33498..34175
                     /locus_tag="ACG85N_000090"
     CDS             33498..34175
                     /locus_tag="ACG85N_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFQ0222900.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            34189..34788
                     /locus_tag="ACG85N_000091"
     CDS             34189..34788
                     /locus_tag="ACG85N_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFQ0222901.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            34869..35090
                     /locus_tag="ACG85N_000092"
     CDS             34869..35090
                     /locus_tag="ACG85N_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFQ0222902.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            35139..35921
                     /locus_tag="ACG85N_000093"
     CDS             35139..35921
                     /locus_tag="ACG85N_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFQ0222903.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            35942..36649
                     /locus_tag="ACG85N_000094"
     CDS             35942..36649
                     /locus_tag="ACG85N_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFQ0222904.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            36911..39709
                     /gene="ileS"
                     /locus_tag="ACG85N_000095"
     CDS             36911..39709
                     /gene="ileS"
                     /locus_tag="ACG85N_000095"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFQ0222905.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(39758..41284)
                     /gene="zwf"
                     /locus_tag="ACG85N_000096"
     CDS             complement(39758..41284)
                     /gene="zwf"
                     /locus_tag="ACG85N_000096"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFQ0222906.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(41299..41946)
                     /locus_tag="ACG85N_000097"
     CDS             complement(41299..41946)
                     /locus_tag="ACG85N_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0222907.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(42130..42459)
                     /locus_tag="ACG85N_000098"
     CDS             complement(42130..42459)
                     /locus_tag="ACG85N_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFQ0222908.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            42636..43364
                     /gene="sfsA"
                     /locus_tag="ACG85N_000099"
     CDS             42636..43364
                     /gene="sfsA"
                     /locus_tag="ACG85N_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFQ0222909.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            43380..44393
                     /locus_tag="ACG85N_000100"
     CDS             43380..44393
                     /locus_tag="ACG85N_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0222910.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            44393..45670
                     /locus_tag="ACG85N_000101"
     CDS             44393..45670
                     /locus_tag="ACG85N_000101"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0222911.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            45733..48435
                     /locus_tag="ACG85N_000102"
     CDS             45733..48435
                     /locus_tag="ACG85N_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0222912.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYDQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            48587..48904
                     /locus_tag="ACG85N_000103"
     CDS             48587..48904
                     /locus_tag="ACG85N_000103"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0222913.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            48934..49254
                     /locus_tag="ACG85N_000104"
     CDS             48934..49254
                     /locus_tag="ACG85N_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0222914.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            49362..50822
                     /locus_tag="ACG85N_000105"
     CDS             49362..50822
                     /locus_tag="ACG85N_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0222915.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            50890..51111
                     /locus_tag="ACG85N_000106"
     CDS             50890..51111
                     /locus_tag="ACG85N_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0222916.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            51142..51324
                     /locus_tag="ACG85N_000107"
     CDS             51142..51324
                     /locus_tag="ACG85N_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFQ0222917.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            51324..51737
                     /locus_tag="ACG85N_000108"
     CDS             51324..51737
                     /locus_tag="ACG85N_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFQ0222918.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            51860..53041
                     /locus_tag="ACG85N_000109"
     CDS             51860..53041
                     /locus_tag="ACG85N_000109"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0222919.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(53112..53276)
                     /locus_tag="ACG85N_000110"
     CDS             complement(53112..53276)
                     /locus_tag="ACG85N_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0222920.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            53430..53503
                     /locus_tag="ACG85N_000111"
     tRNA            53430..53503
                     /locus_tag="ACG85N_000111"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53464..53466,aa:Arg,seq:cct)
     gene            complement(53572..53763)
                     /locus_tag="ACG85N_000112"
                     /pseudo
     CDS             complement(53572..53763)
                     /locus_tag="ACG85N_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT        17853 a         9166 c        11673 g        15362 t
ORIGIN      
        1 ttttagaaaa tttcatgaaa aaggagttag ataggtgttc aaaaattatg tttcctcact
       61 aaaaaaagaa ttttcaggtt atgatctttc aagatttcag aaagatcttt tggctgggat
      121 cacagtagct gctgtagcat tgcctttagc cttggctttt ggggtatcaa gcggagctga
      181 tgcggccgca ggtttgatta cggctatcat tgccggtatc gttattggcg gtttatcagg
      241 cggtttttat caaatatcag gtccgacagg tgcgatggca gctatcttga tgtctattgc
      301 ggcaacaaag ggaatgcaag gtgtattgct tgctactttt ttagcgggtc tatttttact
      361 tgcagcagga cttttccgat taggaacact gacttctttt atcccagcac ccgtaattac
      421 ggggtttact tcaggtattg ctatcattat tgcattaggt cagatcgata atttatttgg
      481 tgttcactcg gaaggtacaa acgttattga aaaactaagt agttatcaaa cgataggatt
      541 tccgataaac acagcctctt tgatcatggg agggctagtc attttaggga tgtttatcta
      601 tccaaaaaaa tgggcgaaac gaatgcctag ttcgctgctg gctatcattt tggcaacagc
      661 agttatgcta ttgttccatt taccagtagc aactgtgggt aagattccac aaacattgat
      721 cagtagtaat cgtttgaata tgggagattt tagcttttct gccttgcaac aagtggcagt
      781 tccagctatt agcattgctt tattaggaat gattgaaagc ttgctatgtg gtgcttcggc
      841 tggccgaatg gcaaataagc ccttagatag caatcaagaa ttaatggcgc aaggaattgg
      901 aaatctgttg cttcctttct ttggcgggat tcctgctact gcggccatcg cccgtacaag
      961 cgtggccatc aaatctggtg cacaaactag attagctggt atgatacatg cagttgtctt
     1021 actgctctcc atgcttgttt ttgcgccagt gatgtcacag attccaatgc cagcacttgc
     1081 aggtgttttg attgtgacgg cttggcgtat gaatgaatgg gaaacgatca aggagctatt
     1141 tactaataaa tattggtcag cagttctatt gtttatcctt acgatgattt gtacagttat
     1201 ctttgattta agtattgcta tcgtgatcgg ggtgatcggc ggctgtgtat tttttgttgt
     1261 caaaagcgct gctatcacga tttcattaga aacgatcgat tggcaaagaa tggggctgcc
     1321 agaaacggaa aagctgaata actggacagt agtgtatatt agtggtccac tgttttttat
     1381 gtcttcagaa aaactcaaaa ataaattgga agaactaaat catcacgatg gtgtcatttt
     1441 ctccatgcga ggcgtaccaa gtattgattt gacggcacgt gcagtttttg atgaatttca
     1501 agaaaaagca gagcaaagag gacaaacgat tatctatact tccttgcagc cagaagtaga
     1561 aaaacaactt tctcacttgt gggaacatac attcacgcat caacatcaaa cagttgctca
     1621 tgcgttgaca gctttacatg cgaaatatac tgcaattagc cattaaaaca atgaaaaaga
     1681 acagacaaga ctgtaaactg aagattttat ggtcttgtct gttcttttta atattgctgt
     1741 aaaagtgaag ctagtacttg ccagtcttga tggataacag gtacaaggga gcgattgtag
     1801 aaaatagctg ctggatgaaa aagtggaaga attcgatagt ttttttctga ccattcgtag
     1861 gcatcctttt gttctgttaa ttgtaagaca ggccctgtat agagttgacc atgatttttg
     1921 gtaaccgtat attctttgcc aagtaaacgt tgaagaccaa tgtttccaag tgtcgcaatt
     1981 aattttggct ggaccttcat cagttcgtaa tccaatattg gtgcatgcgc aaatacttcg
     2041 gaacgagtag gtgtccgatt tggataaaca gtttctgact gttgtgtccg tttattgatc
     2101 cgatgagtca cgcgatacgg gcggctcctt acagcactgg tgatatacac ctcttctcgt
     2161 gttaagccag ttgaagaaag tgcctgcatc aattcttttc cagcttgacc gctaaaagga
     2221 acatgcgtat caatctctgt tttccctggg gcttctccca cgagcatcag tttaggatga
     2281 agaggtcctt gcccagcgat aaatccttct acaggacggc cgttcattct taaagaagct
     2341 tcttgtatca gttcttttgg gtattccatt tatgctcctc ctttttcttt tattataaga
     2401 ataaaagaaa cacaatgcga ctattttatt tggtagaact gtgaggatag aaaagcagaa
     2461 gtattgcttg aatttttgtt ttactttttg attttaaatt tagttgacta acggttgctg
     2521 ttctggtaag ctagtactgt tgtaattgtg gactccacag ctacaaccgc tcagaacaag
     2581 taataagcac agcatatgct gtcacttcgg atggcgagtc taagtatatc agaaggaggt
     2641 gctttatagc atgtacgcaa ttattaaaac tggtggtaaa caagtaaaag ttgaagtggg
     2701 acaagcaatc tatgttgaaa aacttgacgt tgaagcagga gaaaaagttg tttttgacga
     2761 agttatctta gtaggcggag aatctacaaa agtaggcgct ccaactgttg caggtgcaac
     2821 tgttgaaggt actgttgaaa aacacggcaa acaaaagaaa gtcgttactt tcaaatacaa
     2881 acctaaaaaa cattcacacc gtaaacaagg tcatcgtcaa ccatatacaa aagtggttat
     2941 cgacgcgatc aacgcttaat agcgtttgag aaagaaggcc tatatatgat caaaggaaca
     3001 tttaagcgaa atgacgcagg tcagatcgtt tcattcactt tgaccgggca tgctgatgct
     3061 ggtccttatg gaagtgatat cgtttgtgcg ggtgtttctg cgttagcgat cagcactgtc
     3121 aacgggattg cctcattagc cggttttgag ccgattgttg aaatgaatga agaagaaggc
     3181 ggttatcttt ataccgaagt cacttcagga atgacacaag aacaaaacaa tattgctgag
     3241 attctattag aaaatctttt attaggatta caatcgatag aagctgaaaa ctcagaatat
     3301 atccaaatta aaacaattaa tgataaatag gaggtgcaga ccatgttgtt atctatgaat
     3361 ctacaattat ttgcccacaa aaaaggtggc ggttctacat ctaacggccg tgactcagaa
     3421 tcaaaacgtc taggtgctaa acgtgctgat ggacaaacag ttacaggtgg atcaattctt
     3481 taccgtcaac gcggtacaaa aatctatcca ggtgtaaacg tgggtatcgg tggagacgac
     3541 actttatttg caaaagttga tggcgtagta cgtttcgaac gtaaaggccg taacaaaaaa
     3601 caagtgtctg tttatccagt agctcaagaa gcttaattga atcttatgat taaagaggtt
     3661 gtgacaagac ttttgtcaca gccttttttg tttataaaaa taaaagttgt agtggaggct
     3721 acgaagttta ctttgtcttt ttacacttaa tatttttttg ccaatttgga atcaggatgt
     3781 tttgataact atcgttttca attgatggaa gctatattgt ttgctatcag tgaaataatt
     3841 atatggtaaa cagtgttctt tttcttgttt ccattataaa aatcgtatcg ggatttttcg
     3901 ttcttttaaa ttgaacattt ctatctatcc gcgtgttatc aacaaaacaa aatcaggtaa
     3961 atagttgacg aaacagaaat aaatacatag tattatgttt tgaagcctaa gtacaatagt
     4021 ctatagaaaa ggagcgatac cattggcaaa acgcattgac cgaatttata tgtatgtcaa
     4081 agaaaaaacc gctcatttaa caaaggcaga atatgatcaa ggagtaacaa cacaggaaat
     4141 tgctgaagtg ttagggattc agcgaacaaa tagcagtaaa gatctgaatc agctagtgcg
     4201 cgaaggaaaa ctgataaaga cagacggacg accagtacgt tacatatatc aaacactcgt
     4261 tacgcacgta aagccagtta caaaacatgt agtcagttac aaagaaacgc cggtctctga
     4321 agaaaaaaat acactccaaa tagatactaa ggatatcttt gcaaaaatca ttggagctaa
     4381 cggcagcatg aaaaattcag tggaacaggc aaaagctgcg attttgtatc cgccaagagg
     4441 attaaattgt ttgattacag gacctaccgg atcaggaaag acttattttg cacacgcgat
     4501 gtatcatttt gcaaaagcgc atcatgtaat agatgaggaa acggaactga ttgttttcaa
     4561 ttgtgctgat tatgccaata accctgagct tttgatgagt catttatttg ggtacgcaaa
     4621 aggtgctttt acaggcgcag atgaagaaaa aatggggatt atcgatcaag cggatggcgg
     4681 tatgctgttt ttggatgaga ttcaccggtt gccgcctgaa ggtcaagaaa tgatctttta
     4741 ctttatggat catggaactt acagtcgtct tggggaaaca acgaaatccc atgaagcaaa
     4801 tgtacggatc gtaggtgcga ccacagaaga cccaggttct tctctattag aaacttttgt
     4861 tcggcgtatc ccaatcaata tcaaacttcc ggcttttgaa aaacgtcctg caaatgagaa
     4921 agtcgattta gtaaaaatca tgattgccca cgaagcaaac cgcacgcagc gaaagatttc
     4981 tttgacagaa gatgtagcaa aagcattgat cggcagtgtg acctatggaa atataggaca
     5041 attgaagtca aatatccagt tagtatgtgc acgaggattt ctaaatcata tgcaatcacc
     5101 cgaaatctcg atcacaatcg atgatttgac agaagggatt cgtagcggat tgattcaatt
     5161 agcaagcaac cgcagtgcaa tggcagagct ttctaaaata ttggagccaa agataactgt
     5221 agcaccaaat gatacgatca tgaaaatcca atcggactcc tatgagttac cttataatct
     5281 atatgatatc atcggagata aagcagcttt actgaagtcg gatggtttgg atcaagaagc
     5341 aatcaatcat ttcatttcta cagatatcaa catccatctg aagtctttct ataaagacca
     5401 tggtttttct tttaatgcag acagcaaatt agcagaattt gtagatccga aaatcataga
     5461 agtgaccaat cagatttatg taatggtcaa aagatcttta ccatatgagt ttcagcaaaa
     5521 ctttatttat gctatgagct tgcatatcag ttcttttttg aaaaaaatca acttaggaga
     5581 agaacggcat acgaatgata acatccgtga gatggctttt gattttccag atgaatatgc
     5641 tgttgcgaaa gaagtgcgta gttatatcga aacgtatttt caagtggaga ttccagaaag
     5701 tgaagactat tacttgaccg ttctactcgt ctcgttacgg gcaaatcaag caagcggcag
     5761 aatcggtgta gtcgttgcag ctcacggaaa cagcacagca agcagtatgg tccaagttgt
     5821 ccaacagttg ttagatgcag agaacgtacg agcagtagac atgcctttgg atatggatcc
     5881 gaaaacagca ttggaccgaa ttgaacgcaa tgtacaagaa gtagatgaag gaagcggggt
     5941 catactctta gtagatatgg ggtcccttgc gtcgtttaat tcgcaaattc aaagagacac
     6001 tggaattcca gtacgtactg ttgatatggt aacaacttct ctagttttgg aaactgtacg
     6061 aaaggtctca gtattaggga cagatctaga tatgttgcat gaatcattga aaaattttcg
     6121 aggttacgca gaaatatctt ctgtagaatc tgttcaggaa gcaaaaccta ttcataagaa
     6181 aaaagctgtt ttggctatct gcgcatctgg cgaaggtaca gcacaaagaa tcaaagaatt
     6241 gatcgaacga gcggtaaaca agcgtcaaga aacagaatta gaagttctag ctttgtcgat
     6301 tgtggaagtg aaagaagaac tgccagttat ccaaaaagaa tatcagatca tagctactac
     6361 tgggattact gatttgaata tccaagcccc atttattcct ttagaacgtt ttattgatca
     6421 aaatatcgaa gtcattttgg atcagttgct gatggaatca gaattggagg aaactgaatt
     6481 catctcccta gatgaagagt cagctaaaaa tacgtgtgtt gagtttatct ctgataactt
     6541 catctttatt aatggtagta agttaatcga tccaatgtgg caattttcta cacaaatctc
     6601 tcagacgacg ggaatcggcg atgaagaata tggattcaaa atcaacctag tgatgcacac
     6661 agcaggaatg atcgaacgta ttattcggaa tgaaccattg actgtggaag aaaacgaatt
     6721 gacaaataca acgaatgatc ctctttatag ccaattggca gcctcagttg ttctgttgga
     6781 agatcagata aaagtaaaag tgccgataga agaaatgtat tatctgctac gtctagtcca
     6841 taatcagtta gataaaaaag aatacactgt gccatgaata cacaaaaacg atacactttt
     6901 atagtgtatc gtttttgtgt attggttaag aatcctttaa attcaattag tgtatcgtaa
     6961 aaaagttggc acgctttttg cttatagtag tgtgtgctag gaggtggttg ttcacacaga
     7021 aatgataggg gtttctgtga agtagtgagg aagaaggagt tttgcagatg gatattcgat
     7081 tggcacgtat agatgatcgt ttaatccacg ggcaagtcgc aacagtttgg tcgaagcaaa
     7141 caggtgttga acgtatcatt gtgatcagcg atgtagtcgc tcaagataaa ttgagaaagt
     7201 ttctactaaa agaagctgca ccgccgggga tcacagcaaa tgtaataacc gtgcagaaaa
     7261 tgattcaaat ctatcatagt ggattattat cacgagcaaa agtgatgctt ttattcacca
     7321 atccccaaga tgttgaagcg gttgtccgct gcggtgtaca gttgaagtcg ttgaatatcg
     7381 gcggtatgag ttttactgaa ggcaaatcaa tgattaccaa ttttgtttca gtaaatcaac
     7441 aagatattga ttcctttcgt tacttgaaca gtcaagaagt ggagttagag attcgaaaag
     7501 tacctgctga caggaaaatc cttttgatgg aactactagc gaaaatcaaa aaggtgtagc
     7561 aagaatggtt gttggaaaag gtcaaataat tctgttgtaa tggttagcaa ttattaatat
     7621 ttttgaattt gttacaacaa aagtaaatag gaggtataaa tatggtaggg attatcctag
     7681 caagtcatgg acaatttgct gaaggaatct tacaatccgg ttcaatgatc tttggcgaac
     7741 aagaaaacgt caaagccgtg attttgaaac caagtgaagg tccagatgac ttgagagcca
     7801 aattggaaga agcggttgct tcttttgata atcaagacga agtgttattc ttagttgatt
     7861 tatggggcgg aacaccgttc aatcaatcaa acacattatt cgaagagcac aaagataaat
     7921 gggcaatcgt cagcggtttg aatttaccaa tgttgattga agcctacgca tcacgctttt
     7981 caatggaatc tgctcatgaa attgcagcac acatcattga aacagcaaaa gacggtgtaa
     8041 aagtaaaacc ggaagaatta gaaccggcag aagcaccgaa agcagcagtt gaagacgcac
     8101 aacctaaagg agcacttcca gaaggaactg tggtcggaga cggaaaaatc aaatatgtat
     8161 tagcacgtgt tgactcacgt ctgttacatg gacaagttgc gacagcttgg acaaaagcag
     8221 tgcaaccaaa ccgtattatc gttgtttcag atgcagtatc aaaggatgat ctgcgtaaga
     8281 gactaatcga acaagctgct cctccaggtg taaaagcaaa tgttattcct attagcaaaa
     8341 tgatcgaagt agcaaaagac cctcgttttg gaaataccaa agcattgctt ttatttgaaa
     8401 atcctgaaga cgtattgaca gctgttgaag gtggcgtaga catcaaagaa ttaaatgttg
     8461 ggtcaatggc gcattcggtc ggtaaagtcg ttgtaagtaa agtattgtct atgggacaag
     8521 aagacgttga agcgtttgag aagcttgaac aaaaaggtgt gaaattcgat gtacgtaaag
     8581 tacctaatga ttcacgcgat aacatggatg atattttgaa aaaagcaaaa gctgaattag
     8641 caaaagcgta ataaataata ttaaaattta taggaggctt atcatgtcta ttatttcaat
     8701 aattttagtc gtattagttg cctttctagc tggtatggaa ggtattcttg atgaatttca
     8761 atttcatcaa ccactagtgg catgtacatt gatcggttta gttaccggta acctcgaagc
     8821 tggtatcgta cttggcggta cgcttcagat gatcgcttta ggttgggcaa atatcggtgc
     8881 cgctgtagca ccagatgctg cattagcatc agtagcatca gcaattattt tagttttagg
     8941 tggacaaggt gttaaaggtg ttccttctgc gatcgcgatt gccgttcctt tggctgttgc
     9001 agggttgttc ttaacaatga tcgtacgtac aatcgctgtt ccaatcgttc atttgatgga
     9061 tgcggctgct gaagaaggaa acatccgtaa ggttgaaatg tggcacatca ttgctgtatg
     9121 tttacaaggg gttcgtatcg cggtcccagc agcagcgttg ctatttatcc cagcacaaac
     9181 tgtgcaatca ttccttgaat caatgccagc atggttaaca gatggtatgg ctatcggtgg
     9241 gggaatggtc gtagctgtag gttacgcttt ggtaatcaac atgatggcta caaaagaagt
     9301 atggccattc ttcgttattg gtttcgttgt agctgcaatt tctcaattaa cattgattgc
     9361 tctaggtgct ttaggtgttg cattagctct tatttacttg aacctttcta aaatgggtgg
     9421 ttcatcaaat aatggaggcg gaagcaactc tggtgaccct ttaggcgaca ttttgaatga
     9481 ctattaatct tgaaggagga aaataaaatg gcagaagaaa aaatcaaatt atcaaaaaaa
     9541 gatcgtttag ctgttgcgtg gcgctctacc ttcattcaag gttcatggaa ctacgaacgt
     9601 atgcaaaacg gtggctgggc attttctatg attccagcaa tcaaaaaatt atacaaaaca
     9661 aaagaagaac gttctgcagc attaaaacgt cacttggaat tctttaatac acacccatac
     9721 attgcttctc caattttagg ggttacattg gctctggaag aagaacgtgc gaatggcgcg
     9781 cctgttgatg atgtagcgat tcaaggggtt aaagtaggta tgatgggtcc tttagctggt
     9841 gttggtgacc cagtgttctg gtttacagta cgtccaatgc taggtgcact aggtgcttca
     9901 ttagctatgg gaggaaatat ccttggacca atcatcttct tcttaggttg gaacattatt
     9961 cgttgggcat tcatgtggta tacacaagaa tttggctaca aagctggttc taaaattact
    10021 gatgaccttt ctggcggatt gttacaagac gtaacaaaag gtgcttcaat tttagggatg
    10081 ttcgtattag ctgcccttgt acaaagatgg gtatcaatca aattcttacc agttgtatca
    10141 actgtaaaat tagataaagg tgcttacatc gaatgggata aattgccttc aggtggtgaa
    10201 ggaatcaaga gtgcatttga acaagttaat aatggcttag ccctttctcc tgaaaaggta
    10261 acaacattac aagacaactt agaccaattg attcctggtt tagctgcatt aggattaaca
    10321 ttgttatgta tgtggttgct taagaaaaag gtttctccaa tcgttatcat ccttggttta
    10381 ttcgtagtcg gtgttgttgg acacgtcatc ggacttttat aatccataaa aatatagctc
    10441 gggcgttgtc ccgagctttt tttatatagt atactgttca taaacaaata tctggaggga
    10501 gaaaatggtt caatcaatca atacaaaagt agacttggta attgatgcta cttcgtttac
    10561 gggtctgaca gattacgggc aaatcatgat aggtaataat ggttttgagt tttaccatgc
    10621 gcgagataga cgcaaatata tccaaattcc atgggaagaa gtggattatg tactagcatc
    10681 agtcatgcta aagggaaaat ggattcctcg atatgccatt caaacgaaaa aaaatggtac
    10741 atacacattt tcttccaaag aacctaaaaa agtgcttcgt gcgatcagag aatatgtaga
    10801 tgaagataga atggtacaat cattgagttt ctttgatgta gtgaaacgtg gtattcgtag
    10861 tatgtttaaa cgcaagtaaa aaataaagaa aacttctaag aggaggtttt ctttattttt
    10921 ttactttgca gaagaaaata tgaaatagca aaaccaatta tttgtcccct ttaaatccca
    10981 tatgctaaaa ttgattagca aagaaggagg ttatattatg agttggttat ggacattgat
    11041 tgttggagcc gtgatcggag caattgcagg tgcgatcacc agtaaaggaa attcgatggg
    11101 ctgtgtaacg aatatcattg ctggactagt cggttcaagt attggacaag cgattttcgg
    11161 cgaatgggga ccaaaagctg caggtatggc acttattcca tctattttag gcgcagtgat
    11221 tttggtagca gttgtctcat ttttctttgg aaaacgatca taagttttta caaaatagta
    11281 aactaaaagg agttgcagcg agactgtgac tcctttttgg ctttatttat ttaaaataaa
    11341 aagaagtttt ccggaaattt ttatttaact gtcgtgtacg aagaaaagtt tgctataatg
    11401 gaaaaaagcg tgcaaagaaa gagggagttt cattatgatg ctacgtgtag aaaaattgcg
    11461 caaaaaaatg caagaagaaa atttggattc atttttggtt accagtccgt acaatcttcg
    11521 ttacttaacg aactttacag gtacgacagg attagctgtt attactttgg aaaaagcatt
    11581 ttttattact gattttcgtt ataccgagca ggcggcagcg caggctcaag ggtttgaaat
    11641 tattaaaaat gtcggaccta tttttgatga ggtagcaaac ttagtacgca aagaagaact
    11701 gcactcatta gcttttgagg aaacaacagt ctcattttta gaatattcgg ttttagaaga
    11761 aatcatcgat gcggaattag tacctgtttc acagatgatc gaagaactca gagaagtgaa
    11821 agatgaagaa gaaatcgcta ttatcgaaaa agcatgtcat attgcggata tggcatatga
    11881 tcacatcctg aaaatgatcc aaccgggcat gactgagatt gaagtcgcaa atcaactaga
    11941 cttttatatg cgttctcttg gcgcttcagg tgtttctttt gatacgatcg ttgctagcgg
    12001 attgcgttcg gctatgccgc atggtgtagc aagcaaaaaa atcatcgaac aaggtgactt
    12061 gatcacattg gactttggtt gctattacga agggtacgtt tccgatatga cacgtacatt
    12121 cgctattggt gatcctgggg aaaaattaaa agaaatttat caaatagtct tagatgctca
    12181 attagcagtt attgatgctg caaaaccagg aatgactggt gtccaattag atgcagttgc
    12241 tagagattat atttcaaaac atggttacgg ggaagcattt ggacattcta caggacatgg
    12301 gatcggttta gagatccacg aaggaccaaa cgtttcttct cgtgcagaaa agcaatttgt
    12361 accaggaaat gtgatcactg atgaaccagg gatctactta ccaggaatcg gtggagtacg
    12421 tattgaggat gacttgttga tcacagaaga tggaaatcgt gtgttgactc gttcaccaaa
    12481 agaattgatc atcttatagt ttcatcaata gaagaagtaa gcgtaaaaat cagtgttcac
    12541 agactttgtt atagaaaatc actcgattta atgatacact atagaagaac ttttaagctg
    12601 taagagataa taaagttggg tgttttctcc aattcatagc agtttgcagc tgatcggcgg
    12661 cagttttctt accacgtgat cactatgccg catcatagag aattcggatg tatctcgtgc
    12721 ttaggcaaag ggaggaataa ataaatggca gatgaaaaaa atttgatttt aaacgcaaac
    12781 caagatttag gagagatcgt gatcgctcct gaagtcatcg aagtaattat cgggattgca
    12841 gcttctaaag tagaaggcgt atacggaatg cgcggaactt ttgccagcaa tgtcactgaa
    12901 ttattaggac gtgcagctca cggaaaaggt gtttacttag caaacgagga agatggcttg
    12961 aaagtagatc tttactgtta tctagaatat ggtgtttcag ttcctaaagt agcaatggaa
    13021 atgcaggaac gggtcagcca gcaagtcctt ttcatgacgg atatcgattt atctgaagtc
    13081 aatatccatg tagtagctgt tgttcctgaa aaattagaac aaccaacaat cgaagaactt
    13141 tttgaagacg aagaggaaga gaatgaataa agaattatca agacacgaga ttcgggaaat
    13201 ggcattgcag gctttatttc cattagactt caatgcagac ctaacaaaag aagatgcgat
    13261 tttcaacgca atcgaattag accaccgcga catgatcaac gaagatgaat cagagtttgt
    13321 tcctgtttat cttgatacgc tagttggcgg tgtctgtgca aaaaaagatg aattggataa
    13381 agtgattgaa aaacatttaa aaaacaattg gcacatcaat cggatctcaa aaatggattt
    13441 ggtgatcttg cgtatcgcga tttttgaaat gacttatgtg acagatgtac cagcggctgt
    13501 tgctttaaat gaagcaatcg agttggcaaa aacatttagt gatgaccgtt cacgtaaatt
    13561 cgtcaacggt gttttatcca atgtattgaa agaattagaa gctggggcgt aagtcctagc
    13621 ttttttctat gaatcatcat aaacctgtta taaatcggga aaattctatg gtagaatgaa
    13681 aacacaaaaa atatctggga gtggttagat ggcagaattg attaacggaa aagaattagc
    13741 tgaaaaaatg caggcagaga ttgctgaaaa aatccaaaaa ttgaaagata atggtatcca
    13801 tcccggatta gtcgttttat tggtagggga aaatccagcc agccaaatct atgtaagaaa
    13861 taaagaacgt gtagcaaaag aaatcggaat ccattcattg gtagagcgtt atcctgagac
    13921 gatttctgaa gacgaactac ttgcagaaat tgaaaaatac aatcaagatc ctagttttca
    13981 tggtatattg gtccaattac cattgccaaa gcatatagat gaagaaaaga tcctgttagc
    14041 tatccgccct gaaaaagacg tagacggttt tcatccgatg aatttaggca gactccttgc
    14101 gggtaaaccg gacaagattc cttgtactcc atacggcatc atgaaaatgt ttgaagctta
    14161 ttctatcgat ccggcgggta aacgagcagt agttatcgga cgaagcaata tcgtcggtaa
    14221 accgatggct cagctactct tgatggcaga tgcaactgtt actgttgctc attctaaaac
    14281 cgaaaatctg gctgaactgg cacaagaagc agatatttta gtcgtagcaa taggacgtgg
    14341 acattttgtc acaaaagaat tcgtaaaacc aggtgctgta gtgatagatg tagggatgaa
    14401 tcgtgatgct aacggtaagc tgatcggtga tgtgaaattt gatgaagtgg agcctattgc
    14461 aggctggatc actcctgtgc caaagggtgt agggccaatg actatcacga tgctgatgta
    14521 tcaaaccgta aaatctgccg aagcttttgg agcaggtgaa tagatggcac aagaatattt
    14581 aactgtcact acattaacga agtatttgaa aagaaaattt gatgccgatc cttatttaga
    14641 acgcgtatat ttaacaggcg aaatatcgaa tttccgttta cgtgccaatg cccatcaata
    14701 tttcagctta aaagatgacc atgcaaaaat atctgctatt atgttcaaag gagcattcca
    14761 aaaattacga ttccagccaa aagaaggaat gaaagtcctt gtcgttgggc gtatttcatt
    14821 atatgaagcg ggaggttctt atcaaatcta tgtagagcac atggagcctg atggaatagg
    14881 tgctttatat caagaattgg aagaacgaaa agaaaagctg atgaaggaag gcctttttca
    14941 agggcctaaa aaaatgctgc cacgtttccc aaagcgaatc gcggtcctta ccagtccaag
    15001 cggagcggtt atccgcgata tcatcacgac ggtgaaaaga agatatccaa ttgtccaatt
    15061 ggttttgttc ccaacagttg tacaaggaaa ccaagcagcg gacaatgttg tcaggaatat
    15121 ccagagagta gaagagatgg gcaatttcga tacaatgatc atcggtcgtg ggggtggttc
    15181 gattgaggat ttatggccgt ttaatgaaga gcgggttgct cgggcgattt atcaagctaa
    15241 tacgccggtg atttcatctg taggacatga aacagatacc acgatcgctg atttagtagc
    15301 agatgttcgg gctgccactc caacagcagc tgcagaatta gctgttcctg ttttgtcaga
    15361 agagttgatg cgcattcaag aacggcaagc tagattgcag caggcgtttg tacgccagat
    15421 ccagcgcaaa caagaacggt tcgagcgtgc tcgacgttcc tatgtatttc ggcagcctga
    15481 aagattgtat gaaggtcaga ccatcaaatt agaccagttg aaacagcgat tatatcaagc
    15541 tacacagcaa atttatcatg cgaaagaaaa acaaacgatc gcattaagcc accaattgga
    15601 acaagtggct ccgctttatc gagttaaagc agctcgccaa gaaactgctt atctagaaaa
    15661 acgcttagaa gagaaaatcc aacaatatat gaaacaacag cagcagcgtt ttgctcaagc
    15721 tgttcaatct cttgatttgt tgagtccatt gaaaatcatg ggaagaggct acagctatac
    15781 gacgttaaat gatcaagtag taaaaagtgt caccgagatt catcaagggg atttgctgac
    15841 gatccattat caagatggaa tcgttaaagg acaagtacaa gagatagaca aaggagaata
    15901 aaaatatgga aaatccaact tttgaagagt cactgcaaga attagaaaaa attgtcatgc
    15961 agttagaaca aggagacgtt ccattagaat cagctctaga ttcgtttaaa cgaggaatgg
    16021 aattaagcaa acattgccaa gatacattag ctaaagcaga agagaccttg acgaaaatga
    16081 tgacagaatc aaatgaagaa gttgattttg acggaaacga ggaaagctga gatgagatta
    16141 actgactttt cagctattca tctacctgct gtagaaaaag aaatcttgtc ttttttagat
    16201 gagcatacaa cggatagagg actctatgat gcgatgagtt attcagtgaa agcaggcggg
    16261 aaaagaatac gtcccttatt acttttaacg gccgttgctt cctttgatga gcctattgat
    16321 gttcccgtct atcaagtagc agctgcttta gaaatggtgc atacgtattc cttgatccac
    16381 gatgatttgc ctgcgatgga caatgatgat cttcgtagag gaaaacctac caatcataaa
    16441 gtattcggcg aagcattagc tatattggcc ggagatggtt tgcttacagg agcattccag
    16501 ctaatcagta tggcacactt aggtaattct cctaagcttc tgctgcttca gcaattagct
    16561 gtttgtgcgg gatcacaagg tatggttgct gggcaagctg cagatatcga gggcgaatcg
    16621 aaaaagcttt ctttagaaga attagcattt attcatgaaa gaaagacagg gcatttgatt
    16681 cgttatgcct tgctggctgg tggcatatta gcgaagcaac ctgaagaaat cttattgcta
    16741 cttcaacgat tagccgaaca tttaggttta gcttttcaga taagagatga tctactcgat
    16801 gtgattggta caacgaaaac attagggaaa acagctggta aagatgaacg tatggaaaaa
    16861 aatacttacc cgcgtttatt aggcctagaa aagacacgtg aggcgttgga gattgagctg
    16921 ttatccgcca acaagatcat agacaaatta gaagaggacg ttttttcttt tgatggcggg
    16981 ctgatgcgcc agatgatcaa gcaatttgat gtggagaaat gagggaaaga atggaaaaag
    17041 aacgcgtaga tgtattagcg gtcaaacaag gattatttga aacaagagaa caagccaaac
    17101 gttcagtgat ggctggtttg atttacaacg aaaaaaacga gcggtttgat aaaccgggag
    17161 aaaaaattcc agtgagtagt gaattaagag tcaaaggtaa aaaacttcct tatgtttctc
    17221 gagggggatt aaaattagaa aaagcactca aagcatttga tctatctgtt gaaggaaaaa
    17281 cattgttaga tattggtgca tccactggcg gatttactga tgcagctttg caaaatggcg
    17341 caaaaatgag ttatgcttta gacgttggct acaatcagct agcttggaaa ataagacaag
    17401 atcctagagt cgtcgtaatg gaaagagtga attttcgtta tgcaaaaccc gaagacttta
    17461 ccttaggaat tccagaagtt gctgttatcg atgtttcttt tatttcacta aaactgatgc
    17521 tgcctccatt acatgctatt ttaaaagaag agggagaagt gattgcttta atcaaacccc
    17581 aattcgaagc aggcagagaa gctgtaggga aaaacggaat tgttcgtgac cctcagaccc
    17641 atcaaaaagt tttagaagac attttgtcat ttgcggcaca aggtggatat gatgtgctgg
    17701 aattaagtta ttctccgatc acggggggag aaggaaatat cgagttcttg gctcacttga
    17761 ggaaagtacc cgaaagcgga acaatcaatt cagctattaa tatggcagaa gtggtttcaa
    17821 atgcacacga acagtttgac cataaataaa ttgtaactgt gaaaatcaag tgagaagtat
    17881 gcggattctt ctttattata ccaaggaaat aagctatcat tgattaagaa gaatatgcta
    17941 aggagcgaag acgtaatgag aaaaaaagat cggcatcgtt tgattactcg attgttgagt
    18001 gagcaagata tccgaaagca ggaagaattt gttgaaatcc tgaaaagcaa aggaatatct
    18061 gttactcaag ctacgatttc cagagatata aaggaactga aactgatcaa ggtaccggct
    18121 tcagatggcg gctaccgtta tagcatacca gctgaaacaa acgaagatgt aaatactaaa
    18181 ttggaaaaac tattaaaaga cgctttcgtg gcagttgacc aaatggaaaa atttgttatt
    18241 ttgaaaacat tgccgggaaa tgcttcggca gcagcaaatt tgattgataa acgttacaag
    18301 aaagaattat tttcgattat caatgatgac gataatgttt tgatgattac acgaactgaa
    18361 gaccaggcag ttttattgaa gaaagacttc ctgcattacc tttaagttga ggagcgaatg
    18421 tgatgctaca agaaatcagt attactaact ttgcgattat tccggaattg cggctttctt
    18481 ttcacgaagg aatgaccgcg ctgaccggag agactggtgc gggtaaatcc attattatcg
    18541 atgctttagg tctgttagca ggaggaagag gttcaagcga ttatatacgt caaggagcag
    18601 aaaaatgtgt gcttgaaggg ttgtttgaac taccaaaaca agaaggattc tctgaactga
    18661 tggttgaatt agggatcgaa acagatgagg acaatctgat tgttcgacgg gatatgtcct
    18721 taactggaaa aaatgtttgt cgagtgaatg gtcatattat cacattagct aatctgagaa
    18781 gaatcggcag ttatttagta gatatccaag gacaaaacga acatcaggaa ctgctccagc
    18841 ctgaatcaca tcttgcttta ttggatcgtt ttggagatgt agctttccaa cagaagaaaa
    18901 agagttatca gcaagaatat atctcttatc gtgagctgga aaaaagggta cgaaaagtgc
    18961 agcaaaatga aaaatcctat gttcagagaa tagatatgct ccatttccag caagaagaaa
    19021 tagctgctgc tgatttgcaa gtcggagaag aagagaaatt aaaagaagaa agagaaaaac
    19081 ttagcaatta tcaaaaaatt gctgatggat tagctgcagg ttacggagca ctgacgaata
    19141 atgaacaaaa cagtgtagat ggtgttggct tggccgtttc agaactgcaa gggatcgccc
    19201 atctagatgt ggagtatgaa gcaatttatg aaaatatcca aagtgcttac tatttgctgc
    19261 aagatgcaat cggcgatatg agtcgtcaga ttgatttatt ggagttagat gagagtcgtt
    19321 tagaagaagt tacgcaacgt ttagaattaa tccgacagtt gaaacgtaaa tacggcgaat
    19381 ccattgagtc aatattggct tactatgacg aaatcaccga agagctagct tcttctgatt
    19441 tttcagaagg tcaattggac aaaatgaaag aggaactaga gcaaaaagag cttctacttc
    19501 agcaacaagc cgcagatctt catgaagcgc gtaaaaaaat cgcaaaagaa ttggaaaaat
    19561 ccattctgca tgaattgaaa agtctgtata tggaaaacac tgagtttgag gtacgtttct
    19621 taaaagaaga aaatagacaa ttgaatcatg atggatttga ccaaatcgaa ttttacatta
    19681 cgaccaatcc aggagagcca ctgaagcctt tggtgaaagt cgcttctggt ggcgaactgt
    19741 ctcggatgtt gcttgctttg aaaactattt tttcttctga gcaaggtgtg acaagtatta
    19801 tttttgatga agtggataca ggtgtgagcg gaagagttgc acaggccatc gcagataaaa
    19861 tttctcaaat atcgaaatac tctcaagttt tgtgcatcac tcatctgcct caagttgctg
    19921 cagtcgccga ctatcaatat tatattgtga aagcagtgat tgatggacgt acgcaaactt
    19981 ctgtttcaga gttgaaaaca aaagaaagag aagaagaaat tgcacgtatg ctggctggaa
    20041 gtgagattac aaaactcaca ctcgaacatg caaaagaact attgaaactc gcaaaaagga
    20101 cctgagccac ctcgcagtgg ttcaggtcct tttcagcagc tgatagctat attttatttt
    20161 tacatgacac tcattacagc agtcaataca acagatccaa tcgttaaaat cagcattgtc
    20221 caaatgacaa attttgttac tttagcaaaa gtacttgttt gttttttttc acgcattgtt
    20281 ttcaacctcg tcattaatta gttgattcaa gtgtacaatc ttttttccat ttccacaaga
    20341 gggctttttt ataaaaactt cccttttttt aaataatagg tagttacgat acaaataaat
    20401 aatgttaata gaactagcca tataaaggca tcatcccttt cagcaatagg taacttaatg
    20461 ttcattccat agatactgcc gacgattgtc ggtatagtta aaataatcgt taaagaagtt
    20521 aaaattttca taatattatt cagattattc gatacgattg ctgaaaaagt ctgattcatt
    20581 tgttctacta gttttaattg aatgcgagaa gtagtcattg cctgctctgt ttccaccaat
    20641 acatcacgta aatgattttt caaagcatga ttttcttgga aaagtttatt ttgcgctagt
    20701 tctttcaatg catctaaatt agcagaagtc gcttctctga aataaacgag gcttttttgg
    20761 atatccatga tttgataaag ttgtttgttt tcagtggata cttgaagttt ggcttccaaa
    20821 gtatcgcatt gagattgaag cgtagctaaa taactgttgt aagagagtgt gacctgccaa
    20881 atgaattgaa agaagatatt gagattaggc gaaatatcac tttttggaaa ggtctggcta
    20941 aagactttct ttaaaaacag tggttcatga ttcgtaattg tgataagctt ctttttgggc
    21001 gtcaaaataa tagagagcgg gtatgtatcc acttgaaaat aaccgctagg acttgtttta
    21061 gcaaaaggat attgtaacaa taataacgct ggctttgtca actttgattg attgaattct
    21121 tctgtacggg aattttctgc atcgtccaat atggaagtga tgtaatctga cggaatttca
    21181 aattgatgtg tcagctgatg aatctcttct tgattaggac gttctacaca ataccagatc
    21241 gtttggtcag tttcctttcc ggtattacgc agtttctcat tatctaatgt aaaatatttg
    21301 atcatgattt tcctcctgtt gcctgtttct tttattataa cggataggga attggaatac
    21361 atggaatgtg tttgtaagat atagaatata actgcataaa tttaaataaa atgaaacagt
    21421 tgtgtaaagt atatacagta tacttgaagc aacgataaaa aaggagttta cgttcatggg
    21481 cacattcaca ccacttatct tgttttggta tatttacccg gtaatcgttt tgtttggttg
    21541 ccaatttctt gtaaaattat tttcgttaaa tcgtcgtttt aaaataaaag cccctgattt
    21601 ggcgattcct tttttgtggg gcggtatgca tgcattgtct agaaatacag ggacaatttc
    21661 tatcttaccg tttttcttta tttctgtttt gctaatggga atttgtattg ctgttttcca
    21721 agcatattat tatgaagaaa ttatttatcc gcgttatttc aagatgactt ggcgtttggt
    21781 ttttctgttg acaatcgtct tgtacgctgt cttgatcatt gtgaacatac tttcttacct
    21841 atgaaaaaaa tagaaaagct gtaaaaagtc ttttaaaagg ctttttgcag cttgattttt
    21901 aaaaaaatgt ttatgaataa aaaaagttgt tcccggattt cccagaaaag gtggtagaaa
    21961 gtgggggatt gtggtagact aattttagtt agtggaggaa tggggggctt tcaatgttga
    22021 tgggtgaatt ccaacataat attgatgcaa aaggacgttt aatcgtgccc tcaaaacttc
    22081 gtgaagaact tggcgaaaag tttgttctga cacgcggatt ggatggctgt cttttcggct
    22141 atccgatgag cgaatgggaa aatctggaag ctaaactgaa cgaaatgccg ttggctaaaa
    22201 aagacgcacg tacttttgtg cgattcttct attcggctgc cactgaatgc gaacttgata
    22261 aacaaggacg aatcaatata cccagcacgt taagaaatta tgcagctttg acaaaaggtt
    22321 gcgtagtcat cggtgtatct aaccgaattg aaatttggga tgagacacga tggcaagatt
    22381 tttctgcagc agcagaagag aactttgacg aaattgctga aagtatgatt gatttcggac
    22441 tataagaata ggaagaggct gactaatgac tgaaacattt caacactata cagtcatgtt
    22501 gaaagaaacg gtagatggat tgaacatcaa accggatggc gtttatgtag actgtacact
    22561 cggcggtgcc ggacatagtg aatatttatt aagccaacta agttctaaag ggcatctgta
    22621 tgcgtttgac caagatcaaa aagcaatcga tcacgccaag ctgcgtttag cttcttatgt
    22681 cgaacaaggt atggtcacat ttatcaaagc gaacttcaga gaattagaag aacgcttgag
    22741 tgagcatgta gataaggtag acggcatttt gtatgatcta ggggtttcct ctcctcagct
    22801 agatgaagca gaacgtggat ttagttatca tcaagatgca ccgttggata tgcgcatgga
    22861 tcagtcagct cctttatccg cttatcacgt aattaatgaa tattcctacc atgaattagt
    22921 aaagattttc ttccgctatg gcgaggagaa attttccaaa caaattgctc gtgaaatcga
    22981 acgagtacga aaaacaaaac caatccaaac aactggtgag cttgtcgaaa tcattaaaca
    23041 agtcatccca gctcccgcaa gaagaaaagg cgggcatcct gcaaaaagaa tttttcaagc
    23101 tgtgcggatt gcagtaaatg acgaactcgg tgccgaagag gcctctttag aacaggcaat
    23161 ccggttattg aaagtcaatg gaagaatcag cgtaatcaca ttccattcat tggaagacag
    23221 aattgtcaaa agtatgttta aagaatatag tacggtacaa gatctaccac cgggacttcc
    23281 tgtcatacct gacgaattcc aaccagaact gaaagtcatt actcgtaaac caatccttcc
    23341 ttcagaggaa gagctaacag aaaacaatcg atcacgcagt gcaaagttac gtattgcaga
    23401 aaaaataaga atgaaagagg agtagaatga tggcagaatt aaagaaaatg caagattacg
    23461 aatatgattt acctgaattg gtggatcagc cggaacaaca aatgcctgat tcttcttcga
    23521 atcaagaaat cattcttcca caatcaccgg caaaacgatt aaagcagatt tctgctttgg
    23581 aaaaaattgc tgtggcaagc gtggcgattg ctattgttgc tttatgtgtt ttaacagtta
    23641 tgctgcgcac aaatatcagc ggagctgaaa aagaaatcac gtcaatacaa acagaaatca
    23701 atgataaaaa gcaagaaaaa acgagtttag aacaagaaaa aaacgaactt tctcgtactg
    23761 aacggattaa acaaatcgca gaagaaaaag gattgtctat taatgacgac aatttaagga
    23821 aagtgaagta aatgagtctt aaaaataaaa ttcggcggtt tatggaaaag aaaaatctaa
    23881 atccgatgaa caatcgcaaa aaagtaggta tcattttatt tgctacgagt attggattgt
    23941 tctttttatt tgccgtccgt ttctcgtata tcgttattgg aggtcatgtt gctggtacct
    24001 cattagctga aaaaacaaaa cagttgtacc aaggaagtga agtagtaaaa gcaaaaagag
    24061 gaacgattta tgatcgtaac ggtgtggcgc tcgcagaaga tgcctcctct tattcaatta
    24121 aagcgatact ctcaaaaaca tatacctctg gggataaaaa actgtatgtt gaagaaaaga
    24181 attttgataa aatagcagaa atcctgcata agaatctttc tatagacaaa aaagatgcac
    24241 tgaatatttt agaggacggc gcaaaaaaag agctgtatca agttgaattt ggtagctatg
    24301 ggaaaaatat ctcccaagaa accaagcaaa acatcgaagc ggacatgaaa aaagaaggtg
    24361 tcgctggtct ttattttgtc gaccatcagg ctcggatgta tccaaatggc gtcttttctt
    24421 ctcattttat tggatacgca gttccagaca aagatgagaa tggcttagtt ggtaaactag
    24481 gtttggagtc tgcttacaat gatatcttga gcggtaaaga tggtaagatt atttatcaaa
    24541 aagataattt tcaaaatcct ctgccaggta cagtggcaga agaagaaaaa gctgtcgacg
    24601 gacaagatat ctacacgact ttagacagca gactacaaag ttatttagaa acactgatgg
    24661 atcaagtaaa tgaagaatat cagccagaag aattgacagc agtcctgatg aaagcaaaaa
    24721 ccggagaaat cttggcaatg ggacaacgac ctactttcaa tcctgaaaca atggaaggat
    24781 tgactggaaa agatgctatc tggcgtaatt tcttggtaca agatagttat gaaccaggat
    24841 caaccatgaa agttttcact actgcagctg ctatcgaaga aggagaattc aacgagaatg
    24901 aaaccttcca atctggaaaa atccaagtag aagatgctac gatcaatgac catgactttg
    24961 gtgaaaaagg tgtgctgacc atgcgtcaag ctctttcatg gtcaagtaac gtcgggatgg
    25021 tcatactgga acagcgatta ggcggacgat ggtataacta tttacaaaaa ctgggatttg
    25081 gacaaagtac tcattctgga ttagatgatg aagtaaacgg tgctttacct acattaaata
    25141 ttgtcgaccg agcaatgagt gcttatggac aagcggtagg tgtgacgaat ttccaaatga
    25201 tgaaagcttt cacttctatt gcgaataacg gtacaatgat tcaacctcga tatatcagca
    25261 aagtggtgga tccccaaact ggagaagagc gaacaactca aacagaagtt ctagggcaac
    25321 ctttttcaaa agaaacaaca gaaaaagtcc gtgaatatat gagagatgtt gtcgaaagcg
    25381 aaaactacgg tagtgcttat ggtgtatata gtgtacctgg atacaatgta tcggcaaaaa
    25441 acggaacagc ccaaatcgct tctgatacag ggggatatca aacaggagat acagcctacc
    25501 tgtattcgat tgtggagatg gttccttctg aagatccaga ctatgtcttg tatctaacaa
    25561 tgaaacaccc taagacatat gatcgaatgg cattggctaa aatcgcaaat ccattgatga
    25621 aacgggcaat ggattttaaa gaaactgaag aagattcaga tacagaaaca aaaacagaaa
    25681 aagtatccgt agccgattat cgtaatcttg aagcggacgt tgcagcagct gatgctcaaa
    25741 aaagtggact gcaacctgta gtgatcggta atggtaaaaa agtacaaaaa caatctactg
    25801 ccaacggtga tcagttgatc tctggagaaa aactgatcct atatactggt ggtgataagc
    25861 tgatgcctga cgtaacagga tggtcaaaag cagatatcat gaagctgggg aaaatcctcg
    25921 gtattgaagt tagttttgat ggagatggtt attgtgtcaa acaagaactt gctccatatg
    25981 aaaaaatcac tgaagacaaa cttagtttta ctttagaaga ataagaagag aaataggaga
    26041 gaaaagagca tggaatggac acaagcattg attcccattg tcagcagttg tgcaatgaca
    26101 attgccgcaa tgccgctatt tattggttat tttcaaatga aaaagcaagg tcaggcgatt
    26161 cgtgaggagg gaccaaagtg gcacaatagc aaagccggaa ctcctacaat gggcggctta
    26221 gtgtttctga tcggctctat tttgacagga atttgggttg gagcttggca aaaacagcta
    26281 actcctactc ttttcatctt gctttttgta ttagcgttat acggagtaat cggctttctt
    26341 gatgatttta taaaaatatt caagaaaaga aacatgggac tgaactcgaa gcagaaattg
    26401 attggacaga ttctaggggg aatcatcttt tatcttgttt accgttctga aggttatcca
    26461 ggtgtattga atttcttcgg actagatcta cctttaggaa tcatttatgg tattttcgct
    26521 ctcttctggc ttgttggatt ttcaaatgca gtaaatctga cagatggcat cgatggatta
    26581 gttgctggct taggaagtat ctcttttgca gcttacggtc taattgcttg gcatcagcat
    26641 cagtatgatg tattaatcat ttgcttaagt gttttaggag gtttactggg tttcttcgtc
    26701 tacaacagaa aacctgccaa gatttttatg ggagatgtcg gttcattagc gttgggcgga
    26761 cttttagcgg caatttctat aatgctaaac caagaatgga cattgctttt gattggtttg
    26821 atctatgtta tggaaacagc aagtgtgatg cttcaagtta cttcctttaa gcttacagga
    26881 aaacgaattt ttaaaatgtc tccaattcat catcactttg aaatgtgcgg atggtcggag
    26941 tggaagattg atacagtttt ttggttgatc agcattgtga cttcgttgat cacattatgg
    27001 atcgtttggt aggagatgac gggaaatgaa aaaaatatca acatatgaaa ataagaaagt
    27061 gcttgtctta ggactcgcaa aaagcggagt aagtgcggcc aaattattac atgaattggg
    27121 cgcacttgtc accgttaatg atggaaaacc ttttgacgaa aatccggaag cacaagagct
    27181 gctttcttta gggataaaag taataacagg tagtcatcca atcgaattgt tggatgaaga
    27241 attttcattg atggtcaaaa atccagggat tccctacagt catcctttag tagcaaaagc
    27301 ccaagaaatg ggtattccgg taattacgga agttgagcta gcttacgaag tagcggaatg
    27361 cccaattatt gggattacag gaacgaacgg aaaaacaacg acaacaacga tgacaggact
    27421 actactgaat gcaggagcag atcaaggaat tgcacgttta gcgggtaata ttgggtatcc
    27481 agcaagtggt gtagcgcaag aagcaaaatc agaagataaa attgtgatgg aattgtctag
    27541 cttccaactc atgggaatca cagatttccg ccctcatata gcggtgatta ccaatatcta
    27601 tgaagcacat attgattatc atggtacaag aaaagaatat gtcaaagcta aatggaacct
    27661 tcaaaaaaac atgacagaaa aagattatct gatccttaac tggaatcagt cagaactaca
    27721 agaattagcg caaagaacaa aagcacgcgt actgcctttc tcgacaaaag aagtgttgga
    27781 agacggtgtg tatgctgacg attactcaat atactataaa aaagaaaaaa taatggaaat
    27841 atctgaactt ggcgtaccag gtaagcataa tgtagaaaat gcactagctg ctatttctgt
    27901 agcaaaattg tatggtatat caaatgaagc tatacgagaa acgttgcatt ttttccatgg
    27961 cgtaccacat cgtacgcaat atgtaggaga aatccaaggc agaaaatttt ataatgattc
    28021 aaaagcaacc aatattttag cgactaaaat ggctctaagt ggatttgaaa cgtctaaggt
    28081 cgtgttattg gctggcggat tagacagagg aaatacattt gatgaattaa ttccttcatt
    28141 aaaaggaatc aaagcaatgg tcgtatttgg ccaaacaaaa gaaaagttga tggatgctgg
    28201 aaaaaaagca ggcatcgaga caatagtaac agccgattct gtagaacaag cagttccttt
    28261 agcattagaa aacagtacag atggtgatgt agttttatta tcaccagcga atgccagctg
    28321 ggatcaatac ccaaattttg aaactagagg aaacagattc atggaagctg tgaaccgatt
    28381 aaaatagaaa aagtagaagc gagcgataaa aaatgaaaat tttggttaca ggcggaggaa
    28441 cagggggaca tatttatcca gctcttgcct ttgtcaatta tgtaaagact caagaaccga
    28501 atgctgaatt catgtatgtg ggagcaaaac gaggtctaga aaacaaaatc gtccctgata
    28561 caggaatgcc tttccatacc ttggagattc aaggatttaa acgaaaaatt tccatgcata
    28621 acatcaagac ggtccgattg tttcttaaga gtatccgtca agcaaaaaaa atcctcagtg
    28681 attttcaacc agatatcgtt atcggtactg gaggatatgt ttccggagcg gtagtgtacg
    28741 ctgcagccaa aatgggtatt ccaacagtta tccatgaaca aaacagtgta ccaggtgtta
    28801 ccaataaatt tttaagccga tatgtggatc ggatcgctct ttcatttaaa gatgcggctt
    28861 ccttttttcc tgaaaacaaa gcagttcttg taggtaatcc tcgagcacaa gaagtagcag
    28921 atactaaaaa atcagatatt ttgaaaacat ttgatttgga tccagaaaag aaaacagtct
    28981 tgatttttgg cggtagtcaa ggagcactta aaatcaatca agcagtatca gtttttttaa
    29041 gtgaatatat tccttcttct taccaagttt tgtatgcttc aggagataga tattatgaag
    29101 agatcctaaa taaaataaat cctgaaatac aaaatattag tgtccagcct tatatcaaaa
    29161 atatggcaga ggtcatggct tccagtgatc tgctggtggg gcgtgctggt gcaacttcga
    29221 tcgcagaatt cacagctctg gggttaccgg cagtgttggt tcccagccca tatgttacga
    29281 atgatcatca gactaaaaat gcaatgagtc ttgttcacgc aggagcagca aaaatgattg
    29341 cagataatga actaacaggt gaatcactaa gtcagacagt caacgaaatc atgggtgatg
    29401 aagaattgca aaagcaaatg tgccgagctt ctaaagaaca aggaatccct gatgcttcaa
    29461 aacgtcttta tgacttagta aaacaaataa tcaaaaaata aaggcggtgg tcttagtgag
    29521 taagaaaaaa gaaccaaaaa aattgtctca tccgtcttct gatgaaaaca atttgactcc
    29581 ttggcaaaag gctaaccgaa aatatctggc agaacaagag atgaaaaacc aagagccaaa
    29641 aaaagaagac ataacattag atacacccct ttcagattct gaagaagaaa aaacggttca
    29701 agaggaacag caagaagaaa gccagtccga gaaaaaaatc gaattttttg aagagatcaa
    29761 gcaaaagaaa aacggtggac catataacgg ttcttttttg aaccggctgc ccaatttaaa
    29821 aagtcaacgg cacaaagtac tatttcgtcg gttagctttg atcatcactg tattgggtat
    29881 accgctgatt tttttgatct attatgtttc tccttatagc aaattacaag cagttgcagt
    29941 atcaggcaat aagacagtca acagccaaga aatcatctct gatacaaaat tgtcattagg
    30001 agaaaatgtt tggggacagt attttcatcg atctacttat atcgatcgcc ttaaaaaagc
    30061 acagccaaga atcgaaacag caaatataca tttcaaaggg atgaatgaat tcgatttgga
    30121 tgtaacagaa tataaagaga ttgctttgat cgcaaaaaac aatcaatact acccagtgat
    30181 tgaaaatgga acagtattag atgaaaaagt agcaaatcct acaaaaaatc tccctatctt
    30241 ggaagagttc aaagactctg caaaaatcaa agaattaaca aagcaataca atcagttatc
    30301 atcagaatta caaaaagcaa tttctgaaat caagtacacg ccaagagcaa gcaataaaaa
    30361 tctgatccaa ttgaatatga atgacggcaa tcaagttatt gtaaatatca ataatcttgc
    30421 gaaccaaatg aaatattatt cgcaagtagc caaagatatg gatgaaaaag gtgtgatcga
    30481 catggaagtt ggtatcttct cgcatcctta cggtagcact caagaaacgg aagaatcggg
    30541 ccaatcggaa gaaacaactg aaagtgaaga aaatgcggat caaacaaacg aatcaagtga
    30601 agaggcagaa acgcaaaaag cacaaacacc tggatcgtct agcacaaacg gatcatctga
    30661 ttcaaatgat tcaagtgaaa caagtcaaat agaagataat ccaagtacaa ctccgtcaag
    30721 tttaaatgaa aattgataaa acaactttaa aaagtaagaa tgtactttct aaaaaccagt
    30781 aatttatggt agaattggaa gcagtgaata tatctattaa taagtatatt ttaaaacgga
    30841 cgctagttgt tcgcttgtat atggaatagt aagtaggagg gaatcccatt catggcaaaa
    30901 acaggaatgt atgtaggcct tgatattgga acgacatctg ttaaagttgt cgtggctgaa
    30961 tatatcgaca gccaaatgaa tattattgga gtaggaaacg caaaatcaga agggattaac
    31021 cgaggcatta tcgttgatat cgataaaacg gtccaagcga tacaacgagc agtacgacaa
    31081 gcagaagaaa aagcaggtat ccagatcaaa ggcgtaagtg tcggtttgcc agcaaacatg
    31141 cttgaagtag aaaattgcca gggaatgatt gcagttaacg gagattcaaa ggaaatcacc
    31201 gatgaagatg tcagaaatgt ggcttcagct gcccttgttc gttcaatacc tcctgaacgt
    31261 caaatcgtgt caatattgcc acaagatttt actgtcgatg ggttcgaggg aatcaaagat
    31321 cctcgtggaa tgatcggcgt aagattagaa atgtacggtc tgttgtttac aggacctaaa
    31381 acgatcatcc ataacatacg taaatgcgtg gaaaatgccg gattaatcgt caacgaaatg
    31441 gtgattactc ctttagcgct gactgaatca attctttctg acggagaaaa agattttggt
    31501 acgatcgtca ttgatatggg tggcggccaa acaacaacgg ctgttatgca tgataagcaa
    31561 ttgaagttta ccaatcttga ccaagaaggt ggcgaatttg tcactaaaga tatctctatt
    31621 gtattaaata cgtcatttaa caatgcagaa gcgttgaaaa taaactacgg ggatgcatac
    31681 ccagaacgga catcaccaga tgaagaattt ccagtagatg tgattggtca gtcagaacca
    31741 gtgaaagtcg atgaacgcta cttgtcagaa attatttcag ctcgaatgga acagattttc
    31801 aacaaagcaa aagatgcatt ggaccaaatc gaagcattag agttgccagg tggcgtcgta
    31861 ttgacaggcg gagctgctag tcttcctggt gttgtggact tggcacaaga aatcttcggc
    31921 gtaaatgtaa aattgtatgt tccaaatcac atggggctga gaaacccggt atttacaaat
    31981 gtaataagta tcgtggatta ttcagcaaac ttgagtgaag tgtatcaatt ggcaaaaatt
    32041 gctgttacgg gtgaaacctc agcagctcgt caaatggtag ttgaacaaga aacaacaaac
    32101 acatatgaga gctatgaagc acctgaagaa acaatctacg atgagccgga accaaaagag
    32161 tccggagaaa atgtaaaaaa taaaatcaag ggtttcttta caaatatttt tgattaattc
    32221 atcctgggag gaataagata tggaattctc aattgataat aatatcaatg acggcgcagt
    32281 aatcaaagtc atcggagtcg gtggtggcgg tggaaatgct gtaaaccgaa tgattgaaga
    32341 aaacgttaaa ggtgttgaat tcattacagc caacacagac gtgcaagcat taaaaaattc
    32401 aaaagctgaa acagttatcc aattaggccc caaatatact cgtggtttag gtgctggttc
    32461 tcagccagaa gttggacaaa aagcggcaga agaaagtgaa caatccttac gcgaagcttt
    32521 agacggtgca gatatgattt tcatcactgc aggaatgggt ggaggaaccg gtacaggtgc
    32581 tgcaccaatc gttgcaggta tcgcgaaaga attgggcgca ttgactgttg gtgtcgtgac
    32641 acgtccattc acttttgaag gaccgaaacg tggccgtttt gctgcagaag gtatcgctcg
    32701 tttgaaagaa aatgtggata cattattaat tatctcaaat aaccgtttat tagaagttgt
    32761 agataaaaag acaccgatgc ttgaagcttt ccgtgaagca gataatgttt tacgacaagg
    32821 tgtacaaggt atctctgatt tgatcacagc gccaggttac gttaaccttg actttgctga
    32881 cgtgaaaaca gtgatggaaa accaaggtac agcattgatg gggatcggtg tggctagtgg
    32941 agaagatcgc gtagtagaag caacgaaaaa agcaatctcc tctccattgc ttgaaacatc
    33001 tattgatgga gctgaacaag tacttcttaa catcacaggc ggtttggata tgacattgtt
    33061 cgaagcacaa gatgcttcag atatcgtagc aaatgcagca actggcgatg taaatatcat
    33121 tctaggtaca tctatcaatg aagaaatggg cgatgaaatc cgtgtgacgg tcatcgcaac
    33181 aggtatcgat gaatcaaaaa aggaacgcaa gtctagccga ccagctagac aagcacaaat
    33241 gcaatcacct gctcaaaaga ctgtattaga tatggatcaa gctaaaccaa cttcttcaga
    33301 agaagaaaac agttttggcg attgggatat cagacgtgaa caaaatgttc gtcctagagt
    33361 tgatgattcc aattttgaca atatcgaaaa aaaagaattt gatacattca accgcgaaga
    33421 aacaaaatct catggcgatg atgaattaaa tacgccaccg tttttccgtc gtaaaaaata
    33481 aaggagagga agagcacatg attgctgaca acttgcagaa aatcaagcaa gaaattcagg
    33541 attcgtgtgc tcttgttcat cgtgatccga aagaagttac attagtagct gtaacaaaat
    33601 ctgttgaatc cgctcaagct gaagaattgg ttagagctgg cgttatgaat tgtgcagaaa
    33661 accgagtaga taaattatta gaaaagaaac aagatttatt tgattttcct gaaataaagt
    33721 ggcatttaat tggtaattta cagcgcagaa aggtaaaatt gattgtaaat gaaattgatt
    33781 actttcatgc gttggacagt ttaagattag cagaagaaat ccagaagcgg ttagagaaag
    33841 aattatcctg ttttattgaa gtaaatgttt ctggtgagga atcaaaacat ggaatttcac
    33901 cagatgagct tttaccgttt gtggaatctt tagctgcctg cacaaaaatc aaggttgttg
    33961 ggttgatgac tatggcgcct aaagaagctt ctgatgagca aatccataaa gtgtttggta
    34021 cgctgaaaga attgcgtgat cttgttcaag caaaaggtta tgcccatgca ccatgtagtg
    34081 aattaagtat ggggatgagt caagactttc aaattgcgat tgaagaaggt gcaacattta
    34141 tccgtgtagg aacagcgcta ttcaaagacg aataaaggag gaacaaaaat gccgctttta
    34201 aacagaggag caattgctaa ttttttcgga ttagctgatg aggaagagta cgaataccaa
    34261 gattatcctg aaacaacaaa aaaggctgta cagccaacta aaaaaaatac aacacagaat
    34321 caatcaactc attctgtatc tgctgcgaat actcatgaaa aacaaaaaac ggcgacttca
    34381 cgtcctgtaa cgcatgaaag tattcagccg gctgaaaaga aaaaagtaat tgaactacat
    34441 caatcaccta caaaaactgt agcgaaagaa agaacagctt caagaacctc tacacaacct
    34501 aaagcaaatc ttgctggaaa aatcactgta atggaaccaa gagcttattc tgaagctatg
    34561 gcgattgcta aacgaatcat tgctggagaa gcaaccttag tgaattttca tttagtagaa
    34621 gaaaaacaag cgagaagaat cgttgatttt ctaacaggaa cagtttatgc actagatgga
    34681 gatatcaaac gtgttggaga tgagattttt ctttgtacac cgactgggat ggagattgac
    34741 agttcaaccg ctcaatcttt tgcagatgcg aatttgtttg atctgtaaga ggaggaacta
    34801 cgatttatac tattgcacta ttacttcatt tacttcaacg ggcagcctat ttctatagca
    34861 ttatcttgat ggtatatgca ttgctctctt ggtttccagg cgggtatcaa tcagggctag
    34921 gacgattatt agctaagatc tgcgaaccgt atatcagttt gttcgatcgt ctgcctttga
    34981 ggattggacc cttggatttc acaattttag gggcaatcct gatcttgaac ttagccgtac
    35041 aaggtttagc acagatcgtt atatggataa ctgtccattt agtttattga ttgaagcaac
    35101 aaaatcttta ttctcagaat agataggagg ggaaaatttt ggatgcaaat gtataccagc
    35161 actttcgggc agatgaacgt acatttatcg attcggtagg cgattggatt gaacaggttc
    35221 aaggacaata tgcaccctat ctgaccgaat ttcttgaccc aagacaatcc tatattttag
    35281 aaacactgat tcgacaaagc agtgatttgc gtttcatgtt ttatggcgga tatgaacagg
    35341 cagaaagaaa aagatgtttg attttccccg attattacga acctgtagag gaagattttg
    35401 aaacagcatt gtatgaaatc cattatccta gcaaatttgc cagcctaagt catgggaaaa
    35461 tccttggaac tttagtggga acggggatca aacgctctta ctttggcgat atcatctcgg
    35521 acggtgatcg ctggcaagta tttattgcaa atgaggtgga tcattttgtt gctgcacaag
    35581 taacaaagat tggaaaggtt gctgtacgct tggaaccaat caaatacaca gaaatgatta
    35641 tgccgaaaga tagctggaca caagaaagag gaacagttag ttccatgcga ctagacagtg
    35701 tgatctctga gatttataat atttccagac agcggtccaa gcagctgatc gaagctggta
    35761 aagtaaaagt gaactggaca gaaaatacaa gacctgattt tgtacttgaa ctattagata
    35821 ttatttcagt cagaggttat ggcagagtcc agatccaaga acttgagggg aagacgaaaa
    35881 aagaaaagta cagggtactg ttcggtgtat tacgtaaata aaataaaaag gtggtttact
    35941 catggcttta actccattag atatccaaaa taagacattc ccgacaaaaa tgcggggata
    36001 taatcaagac gaagtagatg atttcttaga tttagttgtt cgtgactatg aggaattgac
    36061 tcaaagaaat cgagaattag aaaaagcagt caaacattct gaagaaaaac ttgagtactt
    36121 caatgaatta aaagatgctt tgaaccaatc aatcatcgta gctcaagata cggctgataa
    36181 agtaaaaaca agtgcaagca aggaatccga agtgatcgtt acttctgcac aaaacaaagc
    36241 agatgagtta gttgcgaacg ctgaaaaacg tgcacatcaa ttaacaactg atgcagaaga
    36301 aaaagcaaga aaaatcttga cagatgcaac agaaaaagca agacagcttg cgacagaaac
    36361 tgaagatctt aagaaaaaaa cacgagtatt ccatcaacgt atcagcttga tgcttgagtc
    36421 tcaattagaa caagtgaaaa gtccggaatg ggatgaaatt ttacaaccat tctccagcta
    36481 cgtgactgac agccatgaag tgatcaaaga agttttagca aaacagcttg acaatgaaaa
    36541 tgatactgat gtaaactcag acacaggagt catagaagca cctatgacgg catttgatac
    36601 acaggctatt gatatctcga tcgtgcctgg tgaaaatgaa aacgagtaga acagaaaagc
    36661 actttgttat tcttaaagcg agccgatgag agtggaagtt cggtaagaca ctgcatggtg
    36721 aaagatcctc tacttgagac ctgcttttcc gaaattagag taagaagcag cggctgacct
    36781 cgttattggt cacagagagt ttgcactaga tagatgcaac taaatttggg tggtaccacg
    36841 gcatgttcgt cccttgcggc tgaacatgct tttttgtgtc cggaaaacaa ataaaggagt
    36901 gtatggttga atgaaaatga aagaaacctt gcaattagga aaaacaaaat tccctatgcg
    36961 tggtaatttg ccaaaccgag aaggcgagtg gcaaaaagaa tgggaagaaa atcgaatcta
    37021 ccaaaaaaga caagagttga acgaaggaaa acctagcttt gttcttcatg atggacctcc
    37081 atatgccaat ggtaatatcc atttaggaca ttcattgaat aaaatcagta aagacattat
    37141 cattcgttca aaatcaatgt ctggctatcg tgcaccatat gttccaggat gggatacaca
    37201 tggattgccg attgaacaag tattggctaa taaaggcgtg aaacgtaaag aaatgtccat
    37261 ggcagaatac ttggaaaaat gtcgtgaata tgcactttcc caagtagata agcaacgagc
    37321 agatttcaaa cgcctaggag ttgcaggaga ttgggaacat ccttatgtta cattagatcc
    37381 ttcttatgaa gctgcacaag tacgcgtatt tggtaaaatg gctgaaaaag gctatattta
    37441 caaaggattg aaaccaattt actggtctcc gtcaagtgag tcttctttag cggaagctga
    37501 aattgaatat aaagatgtaa aatctccatc tatttttgta gctttcaatg tcaaagacgg
    37561 caaagatatt cttgatgaag atacagcttt tgtcatttgg acgacaacac catggacatt
    37621 gccagcaaac caaggtattg ctgtaaatcc aaaatttacg tatgtattgg tggaagccga
    37681 tggccgtaag tttgtcgtag cgaaagattt gctagaaaca gtccaatcag aaatcggttg
    37741 ggaaaatgta acagttctaa aagaatttgc tggacaagaa atggaatata tgactgcaag
    37801 tcatccattc tatgaacgtg aatcacttgt gattttaggc gatcatgtta ctttagacgc
    37861 tggtactgga ttagtccata ctgctccagg acatggggaa gatgactata tcgctggtag
    37921 caaatataaa ctatctgtag tgtcacctgt agactcaaaa ggattgttta cagaagaagc
    37981 acctggattt gaaggtatct tttatgacaa agcaaatcct atgatcactg atctattaaa
    38041 agaaaaagga gctttgctga agctagattt ctttactcat agttatcctc acgattggcg
    38101 gacgaaaaag cctgtgatct atcgggcaac tccacaatgg tttgcatcta tcgataaatt
    38161 ccgtcagaat attttggatg aagtagaaaa agtagagtgg gttatcccat ggggtaaaac
    38221 acgtttgtat aatatgatcc gtgaccgcgg cgattgggta atttcccgtc agcgtgcatg
    38281 gggagtacca cttccgattt tctatgctga aaatggagaa gcaatcatca caccagaaac
    38341 aatcgaacat gtggcaaaat tatttgctga acatggatca attatctggt ttgaaaaaga
    38401 agcgaaagaa cttcttcctg aaggatttac ccacccaggt tctcctaatg gcgaatttac
    38461 aaaagaaaaa gatatcatgg atgtctggtt tgattctggt tcttctcatg aagctgtttt
    38521 acgtcagcga ccagaattat cattcccagc agatatgtat ttagaaggat cagaccaata
    38581 tcgtggctgg ttcaactcaa gtatcacgac tagtgtggct atcaatggtg tagcaccata
    38641 caaatcagtt atttctcaag gatttgcttt agatggtgaa ggccgtaaga tgagtaaatc
    38701 tttaggaaac atcattgcac cagaaaaagt gatcaaacaa tttggtgcag atatccttcg
    38761 cttatgggta tctagtgtag attatgaagc agatgttcgt gtttctatgg atatcttaag
    38821 tcaagtggca gaagtatatc gtaaaatcag aaatacgatg cgtttcttat tagcgaatac
    38881 agaagatttc gatcctaaaa aagatactgt ttcgtacaat gatttacgtt ctgtagacaa
    38941 atatatgacc gttcgattga accaagtaat caaagaaatc cgagaagaag ggtacgacaa
    39001 atataacttc atgcatattt accgtacagt tatgaacttc ttgacggttg atctatcgtc
    39061 attctacctg gattttgcta aagatgtagt ttatattgaa gcagaagatg attatcaacg
    39121 tcgttgcatg caaactgttt tctatcaaac agctgtagct ttgacaaaat tattgactcc
    39181 tatcattccg catacagcag aagaaatctg gtcattcctg aaagaagaag aggagtacgt
    39241 tcagttaagc gagtttcctg gttatgaaga gtttgcaaac caagaagaat tgatggatac
    39301 ctggactgct ttcatggact tccgtgataa tgtcttaaaa gcattggaag aagcaagaaa
    39361 cagcaaattg attggtaaat ctttggaagc aaaactaact gtttatccaa aacaacaagt
    39421 tcgtgaaatg ttaaaagctt tggatgcaga tattgcacaa ttattgatcg tttcaccaga
    39481 ctactttgaa gttatggcag cagatgaaca agtgccagat aacgctatgg tatttgatga
    39541 tgttgctatt acagtggaaa aagcagacgg ggaaacttgc gatcgctgtc gccaagttcg
    39601 taaagatgta ggagtagatg aaaaacttcc tcatctatgc ggtcgttgtg caaaaatcgt
    39661 tgaagacttc tatcctgaag ctgtagctga aggattcgaa gaaaagtaaa agggaaaagg
    39721 gggtgtgaca tgttctttgt cacaccccct tttgttatca atcgtttaat tttccacgtt
    39781 cacgatacca gttatctggt tgccagatcc attcgaatcc gtctttttca agtagttcga
    39841 aagctgcttt aggtcccatt gatccagctt tgtagttggg aaaatccggt tgtgtttggt
    39901 cccaagcttt tcgaatgaca tccacgatat gccaagattg agcgacttct tcccaatgag
    39961 agaagttcgt tccatcacca ttcaacgcat ctaatagtaa tttttcgtaa gcttctgggc
    40021 tattttcaac catttctgca ctatttctaa attccaattt gattggttcc gtttcaaatc
    40081 cttgccctac ttctttccca ttcaaggata aagaaaatcc ttcagttggc tgaatataga
    40141 tcgttagaac attaggtgga agtgtcgtat cgtcacatgg ttcatcgaca gatgttttga
    40201 atacattaac aggaacttgt ttgaatacga tgttgatccg agtccctttt tcagtcaaac
    40261 gtttgcctgt acgtacatag aaaggaacac ctgaccaacg gaagttatcg atagtgaatt
    40321 tgcctgcgac gaatgtttca gtctctgatt gagcatccac tttgtcttct tcgcgatagc
    40381 ccttgaattg tacaccatcc aattcacctt gagcatattg cccgcgaacg aagttttcta
    40441 acacttcttc ttcgttataa atgcggatag ctttcaatgc tttgatcttt tctgtacgga
    40501 tttctttttc ggaaaatgct actggcggtt ccattgcaag taatgctaca acctgtaaaa
    40561 tatgattttg caccatgtct ttaagtgcgc cactatgatc gtagtagccc ccgcgatctt
    40621 ctacaccgag gttttcgcca aaagtaattt ggatattgtc gatatagcga ttgttccatt
    40681 gagcctcaaa aatattattg gcaaaacgga tagctgagat gttttggatc atttcttttc
    40741 ctaaataatg atcaatcctg aaaatatctt gttctgggaa tacttctctg atttgctggt
    40801 tcaattcaaa agcagactgg taatcagaac cgaacggttt ttctatgatc aaacgattga
    40861 agccattttt tgtcataata ttttgtgatt tcaaatgaga aacgatcgtt ccaaaaaatt
    40921 gaggtgccat agccaaataa tagacacggt taccgtttaa ttgatagtgt ccatctaatc
    40981 ggtcagacaa ttcttttaat gtctgataat gcgttgaatc atttacatca tgggattgat
    41041 agtaaaaatg gcttgcaaag gtagttgctt cttcatctga aggattcaat gcacggattg
    41101 tttctttgac aacttcacgg tagtgctcat cgctccaggg acggcgagcg gttccgatta
    41161 cagcaaaatg ctcgcctaaa tttccttttc tgtaaaggcg gaacaaagac gggtacaatt
    41221 tacgctgggc taggtcacct gtaccgccga atatcgtaaa caatacattt ttttcattag
    41281 acatgtggat acactccttt atattttatc gacaaaaatc gtgctggccg ccttaaagct
    41341 gacggggatt ttctttctat cattttggat agtgatcggt ccttcataag ctgcgatgtc
    41401 aacgatctca tattcttcgt tgatattcag gtcaatagtg actaagtaat ccaacaattc
    41461 tttttcatca agcactcgtg cgatacgcac ttttgtaccg acaggatagg actccaatgt
    41521 ttgtcggcgt tcttcatgga caacttcctc ttttttagga atcgatcctc catgaggaca
    41581 atgttcagga tgattcaaat agttttctaa atgatctgca agatgttccg aagtcacatg
    41641 ttccaatact tcagcatcat catggacttc attccagcta tagtctaaat gttcaactaa
    41701 aaaaacttcc cataagcgat gtttgcgaat cagactgcta gctttttgca gtccgcttgc
    41761 agtcaattgt acaccttgat aaggggaatg ttctacaagc tcttctttca caagtttaga
    41821 aatcatttca ctgacagatg cagctgagac atctagacta gatacgattt gtttattgtt
    41881 gactttcgtt gtatctccac ctagttctaa aatgattttc aaataatctt ctcgatttgg
    41941 tgtcatattc gatcatcctt tcatggtaaa aaaccatcat tagtaagtgt aacaaaaaaa
    42001 acgttaattg actatttttc taaataaaaa acaaaggatt tttagacgtt tttacaacaa
    42061 aaaacgaaag atgatagaga agactcattc tttcgtttat aaaggatata tatatacatt
    42121 gggagggatc tatttttcag ataacaactt atatgaagtg tctgcagatt cgtcataaag
    42181 agtttgttcg ggataagaaa agataagata aaaaccatac tgatcacgta cgactaaacg
    42241 atattcatct atttctacca cagatgtgcc aagagactga ccgttgatca aaaacgtgta
    42301 atcagatgta atctgtaatt gttgtttttg ctggctatca ctaaagaacc atgtaccttg
    42361 atactttgag agtccttctg acttttccgc ttgttttttc tttttcaagt aaataaaact
    42421 tgagagaacg gtaatgagtg aaccaagaaa atagatcatt ttttttatca ccaaatcact
    42481 cctttatgag aaaataatcg catagacata ttctttcttc tttatttcat tgtaccgaat
    42541 aaaagctaaa atttctctaa gactttcgac tggttttttt actgtttttc tacaaattat
    42601 gataggatac atagtaggaa gaaaggtgac ataatttgaa gtatcagcaa gtggaaatag
    42661 ctttttttat tgaccgcccg aatcggttta tagcacattg tatgaaaaaa gacggagaaa
    42721 taattattgc gcatgtcaaa aatacaggta gaggaaaaga ggtcctgata ccaggcgctg
    42781 aagtggctat tgtatttgct cctggaccta aacggaaaac cgcctatgat ctgattgctg
    42841 taaaaaagaa gggtgactgg tttaatatcg atagtcaatt gcctaatcgg ctggcaattg
    42901 atggtatttt agacggcacg atccttttac caggattaac tggtccattt acggtataca
    42961 agcgtgaagt aacttatctt cactccaaat ttgatattta tttagagacg gtggtcggac
    43021 aaaaagcatt tgttgaagtc aaaggtatga cacttgaaaa taaagcgatt ggtgcttttc
    43081 ctgatgcgcc cacactcaga ggattgaaac atataggaga attaacgtat gccgcacaag
    43141 atggttatgc cgcttatgtc ttgtttattg ctcagttcga acatcttcat ctggcaacga
    43201 tccatgaaga aatgcaacca gcactagctg atatggttcg acatgcgcag caaagcggag
    43261 ttcaaatcct cgcctataat tgtcaagtat cggcagatga ggtaactgtc aagcaggcca
    43321 ttccatttga tgtagatatc ccttttgaag atcctataca atagaaagaa ggtaaaataa
    43381 tgatcaactt aggtattatc ggaacaaatt ggattactca tcaatttgtc caagcagctt
    43441 taagcacaaa aaaatatgaa ttgacagcgg tatattctag acatttggaa aaagcacagg
    43501 aatttggttc tcattatgaa ggggatatcg aattcgcaac agatttgaag acttttttca
    43561 atattactca tatggacacc atttatattg cttctccgaa tagtcttcac tttagtcaag
    43621 ctaagcaagc aattttggca ggaaaaaatg tgatagttga aaagccagct ttttcgacgc
    43681 caaaagaaat ggatgaaatc atccatttgg caaatgaaca caatgtgtat ttttttgaag
    43741 cagcaagaaa tatccatgaa tcaggtttta aaaagattgc cgattactta ccattaaaag
    43801 acgagataat cggtgcaaat ttcagctata tgaaatattc ttcgcgctat gaccaagtat
    43861 tagatggaga agaaccgaat atcttctcga ctcatttctc aggtggtgca ttgatggatt
    43921 taggtgttta ccttgtatat gcagctgttg cttggtttgg aatgccaaaa gaagtccatt
    43981 atttcccaag aaagatttct actggtgtag atggtcttgg atgggggatc ttacgttatg
    44041 agaattttga tgtatcgatc cagccaggta aaatcggtga ttcttattta ccgtcagaga
    44101 tttattttga ctcaggaacg ctagtgatga atggagtcaa tgcaattgaa caagcacaat
    44161 ttcatgatcg cgcgcataaa gaaatcattg atttggcggt aacagctgca gaaaatccaa
    44221 tggcagatga agcagaggat tttgccaatg tcatggctca tccgaaagat ccagcttggg
    44281 gattgttgta tgaagagtgg atcgagctag ctagaaatgt gaaccaagtg atttatgatt
    44341 taagaaaaaa tggcggcatc aaattcgatg cagataatga ggaagatttt taatgaacga
    44401 atttgtacaa gtatttcccg aaaattggca gaaaaaatat gaggataaag ggtttatctc
    44461 accttctctt attcaacgag cagtttttca gccactaagt aataaacaaa gtatagtagc
    44521 ggtttctcct acaggttcag gtaaaacact ggcttatctt tggcctctac tactaaacgt
    44581 ggaaccaggt gaagcaagtg cgttggtcat ttttgcttct tctcaagaac tagctattca
    44641 agtagcagat gttgcacgtg aatggggaaa agacaaagag ttgaaagtcc aatctttggt
    44701 gggcggagcc aacgtcaaac gtcagattga gggtttgaag gaaaaaccag aaatcttgat
    44761 tggtacacca ggaagaatat tggaattgat gaaagcaaaa aaaataaaag cacatcaagt
    44821 gaaaacaatg gtatttgatg aagcagacca gctatttgac gctgggaaca gccaaatcat
    44881 tgatcaaatt cttcatcaag ctccaacaga gtatcagcta gctttttttt cagcgacagc
    44941 agatcgttcc cttgaaagca ttgaaaaaat cactgggaaa acgtttgaga caattgacgt
    45001 gacagcagag gacgattctc gaaaaggact gagacattac tttttacgtg taccgatacg
    45061 aaagaaagat gactacttga gacgtctcac gcatatagaa gattttcatg gtctgatttt
    45121 ctttaatcaa ctgactgaat taggcgtaat ggaagaaaaa ttgctgtata gaggtgtacc
    45181 ggtaggaagt ctggcctctg atcagaacaa gctactccga aaagcagcgc tagaacaatt
    45241 caaaaagaaa atggtcagag cacttttgac cacagatgtg gcggctagag gtttagatat
    45301 tacaggtctt ccttatgtag tgaatgcaga agttccgtta agtgaagaag cttatttgca
    45361 tcgttcggga agaactggtc gaatgggtaa tgaaggaata gtaattacac tcgtacaaga
    45421 taataatcta agagatttaa aacggctttt acgtccatca aatataacac ttgaagagat
    45481 ttttttatac ggaggtaagc tgcaaactga gcagccaacc aaagaagatg cagctcctgc
    45541 agtaaataaa aagtatgcga aaactttttc tggaaaggag cagaacccag aatataacaa
    45601 agaaaaaata agaaaaaaag ttaaaaataa atcaaaaaaa gagagaaata aaggagcaag
    45661 gcgcaaataa gcgccttttt atgctatgat tatgaaaacg ggaacaaaat tggttgccag
    45721 aggaggggtt atgtgaaaga acgtcaagaa gagatcttgc agctattgac agaaaaccaa
    45781 caaggattga cagcaagtga tgtagctgag cgcttaacta tcgatcgaag caatgcaagc
    45841 cgatatttaa gtgaactata caaggcccat catattgtaa aaaccgctgg ccgaccagtc
    45901 gtttactctt tgccaacgga aaagtcaaaa tctgatgaag tgcatgtcga ctcttctacg
    45961 caagtgactt ttgaaacact cgttggagaa aatgattctt taaaagtcag catacagcag
    46021 gcaaaagctg cgattcttta tccaccgcgg gggttgcata cgattatttt tggagaaact
    46081 ggtacaggga agtcgatgtt tgctgaatgt atgtatcatt ttgcaatcga ttcagagatg
    46141 ctttctgcag atgcgccatt tgtttctttc aattgtgcgg attatgatca aaatccgcaa
    46201 ctgctctttg gacacatctt tgggatcaaa aaaggggctt acacaggagc agctcaagat
    46261 agtcccggtc taatagccaa agcagacggc ggtatattgt ttttagatga gatccaccgt
    46321 ttaccgccgg aagggcagga aatgctgttt actttcattg ataaaggtat ttaccgtccg
    46381 cttggagaaa gtgcacaagt acatgaagca tctgttcaga tcattggggc gacgactgaa
    46441 tcttcagaaa gtttcttatc tacgttcaat cgacggattc ctatggcaat caccttgccg
    46501 aatcttgctt cacgttcact ggatgagcgt tatgaaatca tctctctttt tatcaaacaa
    46561 gaagcgaatc gtttgaatca acgtatcgat atcgaaaaag aagcgattct tgccttcatg
    46621 ctttatgatg cggaaggaaa tatcggtcaa gtgaaacgag acttgaaact agtctgtgca
    46681 aaatcttttt tacattaccg tactcatcat gagaaaaagc taatcattcg aaaagaagaa
    46741 ctttcccttc aagtacaaaa aggattactg aaaatcaagg aaataccgga acgtctcgac
    46801 cgattcatgg acagcaaaag tcagtatttg acatttgaac caggatttgc tgatgttgtc
    46861 tggtcacaag atccagaacg aaacatgcag gtctataacg atattgagga aaaagtcact
    46921 tctctttccg aaaccggtgt ggagaacatt gatctagaga ccctcatttc aaaagatgtg
    46981 gatgcttatt tccagacata tgtcaaagaa ttgactaaaa gttcgattcc caaagatctt
    47041 ttgccagatg aaatctggcg gttaaccaat caattgtatg atgtggctga aaaacggtta
    47101 gaccgtattt ataatgaaaa agctcgtttt gcttttgctt tgcatctgca aagtacattg
    47161 gatcgtgtga gagaaggtca tatgatcgtc cacccagatt tgaataacgt acggaaaaat
    47221 tttaagaggg aattccaggt agcaatcgat ctttcaacga tcattgaaga agaagaacaa
    47281 gttgagattc cttttgatga aatcggcttt attagtatgt ttctttctat caatctagga
    47341 gaaagtgagc ctgttaaaga aaacaaagta gatgttctca tattgatgca cggcagacag
    47401 actgcttcaa gcatgttgga gatggcacaa gaactattag gaacaaaagt agggaaagct
    47461 tttaatatgc cactggttat ggaagttcaa acaatgtatg aagaagtgtt acaatatgtc
    47521 aaaaactatc aagagcattt gacgaatggc ctactcttgt taacagatat gggatcatta
    47581 aacaccttca gtgatttgat ttatgaagag actggcattc ggacgaaagc tatcacgatg
    47641 acaagtacca tgattgtgat agaagcgata cggatggcaa gtgttggaag aagcttagaa
    47701 gatatttatc aaaatatcca gctttccttt gaatcgatcg tacgtgatca gttcaaagcg
    47761 gaaaagtctg aaactttaca gcgtaaaaaa gccgtaatcg tgacatgctt tactggtgaa
    47821 ggagtagctg ccaaattata tcagcgtatt atgccagtga ttaatcaaaa taaggtagaa
    47881 ctgatccaga tgcagttttt agaacgtgaa tcattcaaaa aacacatcga tgccttattg
    47941 gaagaatatg agatcaaagc aattgcggga acagttgatg ttgattatca aaatatccca
    48001 tttttttcag cctatgacgt ttttgatgat gaaaaactaa atattttaaa gcgtatagct
    48061 gatgatgaga tcccgacagc taaaattgtc gattcacttg ctggatcatt aagcgatgtc
    48121 ttttctattg atgaactgat ccgttcattg caaaagacag ttcagcagat ccagaccgat
    48181 ttgtcgatta tcgtggaacc tggagtagat actggaatcg ttatccattt agccttcatg
    48241 ttagacggat tgatcaaaca agaaagccat cggcaatttg agaaactgtc tattttctca
    48301 aaaatttatc gtttggaaat ggatatcgtt cgggcaaact tgatggcact agaacgaaaa
    48361 tatcaggtaa aaattccaga agatgatatc gcatttctta cacaaatgtt tcttgaaaat
    48421 aaaataaaaa aatgaacaca ctaatcatac tgtttacaca ctaaacatct gtgtgcgcac
    48481 tataaaaaag agctaaaaag catgaaaacg ctatttcagc tctttttttt attggcatgc
    48541 ttcttgcgtt atataaaagt gtaaaaaaat aaaaggagtg ttaaaaatgg caaaaaaaac
    48601 aatcatgtta gtttgttcag cagggatgag tacgagttta ttggtcacaa aaatgcaaaa
    48661 agcagcagaa gaaaaaggga tggaagccga tatttttgcg gtctctgcct cagatgcaga
    48721 taataattta gaatctaaga atgtcgatgt attattatta ggtcctcaag tacgtttcat
    48781 gaaagctcaa tttgaacaaa aacttgcgcc taaaggtatt ccattagatg tcatcaatat
    48841 gcaagactat ggcatgatga acggagaaaa agttcttgca caagcagaaa atctaatgaa
    48901 ataaatcaag tatgcagttg ggggtataac aacgtggaag atcaacaaaa tctagaagcc
    48961 atcatggggt taatcatgtt tggcggtaat gcaaagagcg atgcaatgga agcgattgcg
    49021 gctgctaaaa tcggtgattt tgagttagcg gataggaaaa tcgctgatgc agaagaatca
    49081 cttatacaag cgcatcatgc acaaacagac atgttgacac aagaagcaca aggaaatcat
    49141 atgcaagtga cattattgac tgttcacagt caagatcact taatgacatc tattgcgttt
    49201 actgatttag caaaagaaat catcgaactt tatcgtcgcg tatacagcga ataaatacaa
    49261 cttctttttt gaacggaaga agaaaaattt ttgaacggat tttttatttt gttataagga
    49321 gaactctctt tatataaaaa acttatagga ggaagtaaaa aatgaacgga ctatcaagtt
    49381 ggatggagaa atacatcctg ccggttgccg caaaaatcgg agcgcaaaaa catttagttg
    49441 ctttgcgtga tgcttttatc ggcatactgc ccgcaacaat ggcgggatca gtcgctgtaa
    49501 tgatcaatgc aattattcgt gatttaccgc aacagtttaa cagtacctat gctgacagtt
    49561 tagcagcaga caaaggaatc tttgccgtat tgaatgtcat catcggaatc aatggttttg
    49621 tctggaacgg tacactagca atcgccggat tgatctttgc cttttcttgg gggtataatc
    49681 ttgcccgagc ttatggagta aatgatctag ctggggggat cgttggttta gctaccttga
    49741 ttcaaggtat ttcctttggt ccagcattat cagcagcatt agatgtgaat gttccttcaa
    49801 atgtcgcaga tgcaatcaat agttctgaga ttggtgcaac gattgcagat ggcacactgt
    49861 cagttggatt atggggttgg ctaaaactag atcatttgaa tggaaacgct tattttacag
    49921 ttatgatcat gggagctatt tcagtcatta ttttttgtaa attgatgctt gccaacatta
    49981 cgatcaaaat gcctgattca gtaccaccag cagtatctaa agcatttgca gccattttac
    50041 cagcgacgat cgctttatac gtcattgcta ttatcaatta tatcgtaggt cgtgtagctg
    50101 atgggcaact gatcattgat ttggtgcaaa aatatattgc agaaccattc ctaggattat
    50161 ctcaagggtt gggagcagta ttgatcgtca cagtatttgt tcaagtattc tggttctttg
    50221 gtattcatgg atcaaatgta ttggcaccag tgctagaggg aatctgggga caagcacaac
    50281 taatcaatat tgatattttc caaaaaggtt ataacggaaa aactggtaca ccagccgtgt
    50341 tagatgcaat cgaagatgga aaagcttata tgtgggtccg cggatcgttc gatgcttttg
    50401 catggtttgg cggatcaggc ggaacgatcg tcttactgat agctatcttg attttctcta
    50461 aacgtgcaga ttatctaaca gtagctaaat tatccattgg gcctggtatc ttcaatatca
    50521 acgaaccagt gatgtttggt ctgccaatcg tattgaatgc aatcatgttt atcccattct
    50581 tgattgctcc agtagtagca acagcaattg gatggacagc tacttatctt ggcctagtcg
    50641 ctccggtatc gcagcaagtc acatgggtag taccgccgtt cttattgtca ttcttagcaa
    50701 caggagcgga ttggcgggca ccgatcgtaa cattagtatg tatggtcgtg actttcttga
    50761 tttgggcacc atttgtcatc tctgcaaaca aaatggaccc tgccgcaggc gaacaacaat
    50821 aagatgaacg tgaggagcgg gacgctttaa gtttcgttcc tcataatgaa ttttcgagga
    50881 ggaactgaaa tgattacaat tgccagtgca atgcaacaag atgaaatcaa agaattgtta
    50941 gaaggataca atgaagaagg aatgactttt acatttaaag aaaaacaagg aatcaagctg
    51001 ttctttgaaa caagtgcaga agatccggaa gttgcggcta aaaaagcaaa ggaactaatc
    51061 aaagcacagc cttggggaac agtcctttat tttcaatcaa cagctgttta gaaaatggtt
    51121 taaaaagata ggatggttga gatgataaaa aacggtttat ttttatgtag tattggttct
    51181 ttgatcgtgt tgtatagttt gaatccacat tcgcaaaggt atgatttgta tagtatggtc
    51241 acaggtcttt ttctaacagg attaggaatc tatttcttca tgaaggggaa aaaaagagaa
    51301 gaagcaaagg agaaaaataa ctaatggaaa tcacgaaaaa attaaatatt ccagcatcgt
    51361 ttttttacga taaagttatg gactctgtcc ttttcgatgt acgtaaagcg accggcaaat
    51421 cagtaactag aaaacagctg aaaaatttgg agtacgtcaa acaattctcg caaaataacc
    51481 gggctcgcat aaaaatcgaa gaagtcattg aaaaccagtc ttataagttt cgtacatcga
    51541 caacgaagaa tgaattcgtc gttcattatc aaatcaaacc attggacgat tcgacctgtg
    51601 aggttcatta cacagaaaaa atggagtctt atggcatgct tcaaaagttg aatgatatgc
    51661 ttttaggaac aatcttgatg tattttaaac ggagacgttt caaaaaaatg ctagatatga
    51721 tcgaagcaac gtattaaatc aatgaaacat ggtactattt agttattgaa aaatgaagta
    51781 agctagaagg aatttttcat aggaagatag atctttcctg cgaatcctca ctagcttttt
    51841 tgtaggaagg ggcagcaaaa tgacaaacaa agtaagagag ctaggtgtat cttcgattga
    51901 ggaaatgttt gatttagcca cttatgcatt taatggggca gatacacctg aaaggcgaga
    51961 acgttttcgt acacttgcgg agaattcttg gaattacgga ttttttgatg aggaggatcg
    52021 gctaacaagt caagtcatgg cgaccccttt tccagtgaat ttttacggac aagagtacct
    52081 aatggccggc attggctatg ttgcttctta tccagaagca aggggacaag gcgggatcaa
    52141 tcagatcatg gaaaagatcc ttgaagattg ccgtgatcgt aaagtaagct tatcttatct
    52201 ggcgccattc tcctatccct tctatcggcg ctatggctat gaacaatcat ttgataaaat
    52261 cagttatcaa ctaaacagtc gtgatgtgcc gtttatcaaa aaaaaatctg gaaaaataaa
    52321 gcgtatggat tttgaagatg caaaagctgc aatcagaaga atttatgaac agatgccgga
    52381 aaaccaacga gctggtctga agcgcgaaga ctggtggtat acgtataaat tcaagcaaaa
    52441 aggcaattac cagtttgctt tgcattttga tgataatgag gaagcaacgg gatatatcgt
    52501 ttatcagctt gcaacgtctt cctttattat tgcggagtgg ggatttttgg atcatgatgc
    52561 tttttgcagc cttgttcgct tcgtaagttc tcacaatggt gcttttgaaa catttgaata
    52621 ttcatgcggc tacggcggta atgatcaaaa ttatctatta gaaaatccat ttgcttctgt
    52681 aatgatcaca ccttatatga tggcgaggat cgtagatatt tctttgtttt tggaaaatta
    52741 tccatttaaa aagagacaag ctgcatttgc cctacaaata gaagaagatc attatgcgaa
    52801 atggaatgaa ggaatcgtgg aagtaacaat cgaaaatgga aaaaatcatc tctcaaaagt
    52861 agaacaaaca aaactcccaa tcgttcgagg aagtatccaa catatcacgc agctattatt
    52921 agggtatcgt aaagtggaag agttggtatt ccaagaaaga atacaggtga caaaagaata
    52981 tgaagaaacg ttaagctcaa tcttgccaca gcaacagcca attcttaatg actatttttg
    53041 ataaaaaacg ggtgatgaca atatttttgt caccaccctt ttgtttttca tttggcattt
    53101 gaaggaaaat gttaaagtaa atcctctttt ttcgtaaatt tgaatttttt gttttctttt
    53161 gttttagggt tttctttttt gtggtcttgg attgcttctt tcgtaatctt ttcccgattt
    53221 tttttagatg aatctaagcg ttctgcgtat actttttctt ttggcttatc attcattgga
    53281 aaggacctcc ctacattatt tcattctacc tctagtttat catctgttaa ggactagtgg
    53341 aaacgttttg cctaacttat aagctgattt ttttagcaga taagcttgca agtaaaaaac
    53401 aaacaagcta caattgattt attgaaattg ttcctgtagc tcagtaggat agagcaatcg
    53461 cctcctaagc gataggtcgc tggttcaatt ccagtcagga acgtataaat aacccctgta
    53521 tcctttgcgg tacaggggtt tgttttgtat ttgtgccaaa aatgtgccaa actagttatc
    53581 atttttatat aaactatcga acacagtatt gattttttct ttatcctgat cttcaagctc
    53641 tcgaacaata tgaagataag tagacattgt agtttctaaa ctgctatgtc ctaaacgttt
    53701 cgatacgcta agtatattta ctccttgata aagtaaaata gatgcatgcg tatgtcttag
    53761 tccatcaata aacagtcgaa gagaaaatag ggaaaaattg attactaaag aaaaacttac
    53821 taggtcgacg gtgtagttga ttttcatact tcaatagatt cgaattaaac gatttcgatt
    53881 ggcttatacc agtcctatcc aacgaaagat atatcgttct taactctagc ggtataactt
    53941 gaagctttac gtaaagccaa tgggaatgaa ctggagatga ctttttatta tttttagtaa
    54001 tagctgatgg gttaatactt acaactttcc ttttcaaagt accatataaa aaaa
//
LOCUS       DAZKLA010000003        53719 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000003 DAZKLA010000000
VERSION     DAZKLA010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.003"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            160..840
                     /locus_tag="ACG85N_000113"
     CDS             160..840
                     /locus_tag="ACG85N_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0222921.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            930..1964
                     /locus_tag="ACG85N_000114"
     CDS             930..1964
                     /locus_tag="ACG85N_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0222922.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1978..2313
                     /locus_tag="ACG85N_000115"
     CDS             1978..2313
                     /locus_tag="ACG85N_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0222923.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2297..4777
                     /locus_tag="ACG85N_000116"
     CDS             2297..4777
                     /locus_tag="ACG85N_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0222924.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4833..6392
                     /locus_tag="ACG85N_000117"
     CDS             4833..6392
                     /locus_tag="ACG85N_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0222925.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQISYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6389..7753
                     /locus_tag="ACG85N_000118"
     CDS             6389..7753
                     /locus_tag="ACG85N_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0222926.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7924..8994
                     /locus_tag="ACG85N_000119"
     CDS             7924..8994
                     /locus_tag="ACG85N_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0222927.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9009..9677
                     /locus_tag="ACG85N_000120"
     CDS             9009..9677
                     /locus_tag="ACG85N_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0222928.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9697..10275
                     /locus_tag="ACG85N_000121"
     CDS             9697..10275
                     /locus_tag="ACG85N_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0222929.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10676..11587
                     /locus_tag="ACG85N_000122"
     CDS             10676..11587
                     /locus_tag="ACG85N_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0222930.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11965..12882)
                     /gene="rnhC"
                     /locus_tag="ACG85N_000123"
     CDS             complement(11965..12882)
                     /gene="rnhC"
                     /locus_tag="ACG85N_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFQ0222931.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13084..13521
                     /gene="zapA"
                     /locus_tag="ACG85N_000124"
     CDS             13084..13521
                     /gene="zapA"
                     /locus_tag="ACG85N_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFQ0222932.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13579..14127
                     /locus_tag="ACG85N_000125"
     CDS             13579..14127
                     /locus_tag="ACG85N_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFQ0222933.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14242..16602
                     /locus_tag="ACG85N_000126"
     CDS             14242..16602
                     /locus_tag="ACG85N_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFQ0222934.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16689..17003
                     /gene="trxA"
                     /locus_tag="ACG85N_000127"
     CDS             16689..17003
                     /gene="trxA"
                     /locus_tag="ACG85N_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFQ0222935.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17185..18963
                     /gene="uvrC"
                     /locus_tag="ACG85N_000128"
     CDS             17185..18963
                     /gene="uvrC"
                     /locus_tag="ACG85N_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFQ0222936.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19108..20007
                     /locus_tag="ACG85N_000129"
     CDS             19108..20007
                     /locus_tag="ACG85N_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0222937.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            20000..21262
                     /locus_tag="ACG85N_000130"
     CDS             20000..21262
                     /locus_tag="ACG85N_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0222938.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21423..23321
                     /locus_tag="ACG85N_000131"
     CDS             21423..23321
                     /locus_tag="ACG85N_000131"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFQ0222939.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23651..24370)
                     /locus_tag="ACG85N_000132"
     CDS             complement(23651..24370)
                     /locus_tag="ACG85N_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFQ0222940.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    24560..24607
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            24707..25039
                     /locus_tag="ACG85N_000133"
     CDS             24707..25039
                     /locus_tag="ACG85N_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0222941.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25026..27029
                     /locus_tag="ACG85N_000134"
     CDS             25026..27029
                     /locus_tag="ACG85N_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFQ0222942.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27048..27518
                     /locus_tag="ACG85N_000135"
     CDS             27048..27518
                     /locus_tag="ACG85N_000135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFQ0222943.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27539..28123
                     /locus_tag="ACG85N_000136"
     CDS             27539..28123
                     /locus_tag="ACG85N_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFQ0222944.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28138..29124
                     /locus_tag="ACG85N_000137"
     CDS             28138..29124
                     /locus_tag="ACG85N_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFQ0222945.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29114..29425
                     /locus_tag="ACG85N_000138"
     CDS             29114..29425
                     /locus_tag="ACG85N_000138"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFQ0222946.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29447..31228
                     /locus_tag="ACG85N_000139"
     CDS             29447..31228
                     /locus_tag="ACG85N_000139"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFQ0222947.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31221..32597
                     /locus_tag="ACG85N_000140"
     CDS             31221..32597
                     /locus_tag="ACG85N_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFQ0222948.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32604..33239
                     /locus_tag="ACG85N_000141"
     CDS             32604..33239
                     /locus_tag="ACG85N_000141"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFQ0222949.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33269..34624
                     /locus_tag="ACG85N_000142"
     CDS             33269..34624
                     /locus_tag="ACG85N_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0222950.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34816..36210)
                     /locus_tag="ACG85N_000143"
     CDS             complement(34816..36210)
                     /locus_tag="ACG85N_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0222951.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36302..37273
                     /locus_tag="ACG85N_000144"
     CDS             36302..37273
                     /locus_tag="ACG85N_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFQ0222952.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37270..38052
                     /locus_tag="ACG85N_000145"
     CDS             37270..38052
                     /locus_tag="ACG85N_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFQ0222953.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(38221..38592)
                     /locus_tag="ACG85N_000146"
     CDS             complement(38221..38592)
                     /locus_tag="ACG85N_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0222954.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38726..39169
                     /locus_tag="ACG85N_000147"
     CDS             38726..39169
                     /locus_tag="ACG85N_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0222955.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="ACG85N_000148"
     CDS             complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="ACG85N_000148"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFQ0222956.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(40040..41407)
                     /locus_tag="ACG85N_000149"
     CDS             complement(40040..41407)
                     /locus_tag="ACG85N_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0222957.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41391..42221)
                     /locus_tag="ACG85N_000150"
     CDS             complement(41391..42221)
                     /locus_tag="ACG85N_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFQ0222958.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42384..42821
                     /locus_tag="ACG85N_000151"
     CDS             42384..42821
                     /locus_tag="ACG85N_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFQ0222959.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42897..43517
                     /gene="rpoE"
                     /locus_tag="ACG85N_000152"
     CDS             42897..43517
                     /gene="rpoE"
                     /locus_tag="ACG85N_000152"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFQ0222960.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            43706..44212
                     /locus_tag="ACG85N_000153"
     CDS             43706..44212
                     /locus_tag="ACG85N_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0222961.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            44229..45128
                     /locus_tag="ACG85N_000154"
     CDS             44229..45128
                     /locus_tag="ACG85N_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFQ0222962.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            45215..46099
                     /locus_tag="ACG85N_000155"
     CDS             45215..46099
                     /locus_tag="ACG85N_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0222963.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(46291..46362)
                     /locus_tag="ACG85N_000156"
     tRNA            complement(46291..46362)
                     /locus_tag="ACG85N_000156"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46327..46329),aa:Arg,seq:ccg)
     gene            46422..47756
                     /gene="rpoN"
                     /locus_tag="ACG85N_000157"
     CDS             46422..47756
                     /gene="rpoN"
                     /locus_tag="ACG85N_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFQ0222964.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            47905..48996
                     /locus_tag="ACG85N_000158"
     CDS             47905..48996
                     /locus_tag="ACG85N_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFQ0222965.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            49037..50038
                     /gene="gap"
                     /locus_tag="ACG85N_000159"
     CDS             49037..50038
                     /gene="gap"
                     /locus_tag="ACG85N_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0222966.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            50148..51338
                     /gene="pgk"
                     /locus_tag="ACG85N_000160"
     CDS             50148..51338
                     /gene="pgk"
                     /locus_tag="ACG85N_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFQ0222967.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            51442..52197
                     /gene="tpiA"
                     /locus_tag="ACG85N_000161"
     CDS             51442..52197
                     /gene="tpiA"
                     /locus_tag="ACG85N_000161"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFQ0222968.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            52313..53611
                     /gene="eno"
                     /locus_tag="ACG85N_000162"
     CDS             52313..53611
                     /gene="eno"
                     /locus_tag="ACG85N_000162"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFQ0222969.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        17827 a         9355 c        11351 g        15186 t
ORIGIN      
        1 ttaataaaaa aggaagaata ttcatgtaaa cgattttatt gtttttttct cttttataca
       61 aaaagggtca taatttcaag taagcggatg aattcatgag gattttttgc atactgtctc
      121 tactatactt aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa catgattggg
      181 acattattac tttcaacact tcttctaggc ggcttggcaa caccagcttt tgctgagggt
      241 caagcgactt caaaaggaga tattaccttt actgaaccaa caaataccgt ggagcctctt
      301 aaccctactg atccttcaaa accagtagag ccagcagatc cggaaaatcc agcaactggc
      361 cagacaggtc ctttaacact ggatgtagtt cccgaattgc catttggtac acacgagatt
      421 gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa acgatacacc ttatctccaa
      481 gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa cgtgacagtt
      541 tcggtttcag attttgtaaa cggttcacaa gttttgcaag gcgctgaatt agattttgga
      601 acatccactg ttaaaagtac gtcagataac gaatccactg gaccaactag tcaaactgta
      661 actgggttga gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa agatcaaggt
      721 ctgggaacat ggctttctgt ttatgatcca gcaaatatca cactgaaagt gccgaaagca
      781 gcagctggaa cattcacggc tgatttgact tggaacctcg tggctggccc agtagcatag
      841 agtatgaaat aaattcaaca aactgcggtg aaaatactat aggtgaagga ttttttaccg
      901 cagttgttgt tgaaaggatg tggtggacaa tgaatcaaag taaacgactg tttgtttcgt
      961 ttttttctat tttaagtcta tttttcattt ttccgtcaat cagccaagca aaggattcag
     1021 caggagactt tggaatcaaa ccggtatttc cagagaacca aatagataaa gcaatcggtt
     1081 attttgattt attagtggca ccggaacaaa atcagacact agaagtaatt atcagcaatt
     1141 cttcagatga agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg agtgatggtg
     1201 gaacgatcga ttatagtcaa aaaaacccaa cgttagatga gacattgccg tttgatgtcc
     1261 gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca gaaacaactg
     1321 tacctataga agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta gcgggtattc
     1381 atgtttcacc taaagaagaa gctgaaacag aaaatgctac agaaggagca caaatcaaaa
     1441 atcgtattgc ttataatctc gcagtcgttt tacaagaaag tcaagagacg atagaaccgg
     1501 atctgaaact tttatcagga gatttggacg aggtcaacgc aaagccaact gttcagcttc
     1561 gtttccaaaa tcctcagcct aggattatca gcaatttgat atttacgagc aagatctttt
     1621 atgaaaatca gctttatatt gaaaatacgt ctaatgcatt tttagttgca ccaaattcga
     1681 attttcactt gaatcttgat ttagctggtg ataaagcaaa agccggcgat taccgcgcag
     1741 aaatcatagc aaaatcaggc gacagtaatg aatggcgatt tacacaaaac tttacgatca
     1801 aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa gaacaatctt
     1861 tcccatggct ttatgcagga ttggttgctg cagttctagt gttatggttg attttgctgt
     1921 ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt gatttcgttg
     1981 aaaacatcga tcttattggg agggattatt gcaagtttgc tgttcttcag ctggcaagag
     2041 ccagtccaag cagataccaa aagttcaggt acgattacat tttatgatct aagtccaatt
     2101 ccgcatccaa gtggttcagt caatggcgca tcaaaacagc agacacagct accgatttat
     2161 aaagaatcta atcaacatgg cgtattcccg gagacgaatg aacaaaagat ccgttttctt
     2221 tatttgatca ttggtataga attatggctt cttgcgtggt tgttgttatg gagaagaaag
     2281 gaaaaggagg atgaacatgt tgctacaaaa taaattttca ttttttcgct ccttcttttt
     2341 tacttcgctt tttgttttaa gtatatttat cttttcacct tcggcagaag ctgttggagc
     2401 aacgcctcct gccatttctt atgatccata cacgtatgaa aatatcgtac ctccaaatgg
     2461 tggacgtagt gcagtcaatg gcgggaagct tgtccaacta actggggcta accaattgcc
     2521 taaattgact gctacttatt tgaacgatgg ttcaaatgat ccttggaaag gtggcacggt
     2581 ccgttttcat tgggaaagcc atgtgaaact aacaaatgta cttgatttgg tgaatatcac
     2641 tgcacaagga tttgcatttg tggcgatcat caatttgccg gaacatgtat caagcagtga
     2701 tgtattaaaa gcaattgact ggaacaacgc ctacttgtat attgccggtg cccccatcaa
     2761 ggttactagc ggaagtttat tatcagcagg aagccatacc ttgcgttttg ggatggggtc
     2821 gtgggataca aatactctat tgacagatat tattgctatt attacgcgag gcgaattcaa
     2881 cgtggacgat atcccgcttc aatttgatgt tacagctaat gtcgcgaaga tgacagaaaa
     2941 cggagataaa tatgatacga gtccaaacaa aattttgacc aagggtaaat tacaaccttc
     3001 tatgtctaaa aaggccgttt tttctgttga tttttatgat tcgaataata ttatccaaga
     3061 tcgtatgttc aattcgattt tagctccagc tgcaggtaga ttttatccta aattaactgg
     3121 cggaaacttg aatccaggag catatgcatt ttctgcaagt gcagagataa atacgtggaa
     3181 tagttacatc tcaccaagtg attcaacagg aaaatataat acatttgctg ataccacaga
     3241 atacgtggat ggaaataacc agaaccttcc aggctctacc aatagtcgga cattgacgat
     3301 ggggctgact gaaggacgtt ttgatcagtt gccatttagt cgctttgatc gcgtagtcaa
     3361 ctattttaca ggaaaaaacg ttacacaagg agcaatactg actcatactc cagcaagcac
     3421 aagtggattg aatcagacag ccaatatcgt gtacagtggg aaagatgcgc aaggcggatt
     3481 gctttctcct gttgctttaa aggtattgga gcagtacaag ctagacggaa cggttttacc
     3541 aacaaactta acagccgata gtcagtgggg aaagctcctt tcttctgcat cgtaccaagt
     3601 cacggccaat tggtcagcaa ctgaactaaa aaagggttcg atccattaca ggctttattc
     3661 gaaaggaacc caaaaattag cgacaggatg gacagatcaa cttttccaaa cgatccaatc
     3721 aaataacggg ggtgtaaata cagctacttc tacactacct gcattggctt cagggcaata
     3781 ttattttgat tatcgcttgg tggacgatgt acttactaac atgtatccgt cttttgcgta
     3841 caaatggcag tctgaacaag ctggaatcac cactcttcct caaataacag tggctaattt
     3901 tccatctgtg agtgcagata gtcagctaaa aaaccttagc agacaacctg agacaggaga
     3961 tcccctgtct gctctatcag gagatacgat tcaagaaaat gtgacattca cgttaacaaa
     4021 atctggggat gcgataaccg acaaaaaaat cacgatctca cttcctgaaa atactactta
     4081 tatcaatggt tccttaaaat taaatggtac aactatcgca gataccggca ttcaacaagg
     4141 agtttctgtc ccagcagatc tattgagcaa aataggagat accattcatc ttacttacaa
     4201 ctatcaatta aatactgtag atacttcttt acaatccgtc agcatactga caaaggcagc
     4261 tgtactttcc agcaatatta cattagcaga tggtgctaag cttcctaacc cagttgtcca
     4321 aacatctgct aaaacaattc ttgtccccaa acaagaatta acattagtta atgtaccaga
     4381 tgattttaca tttggtaacg atctccccaa accgttgaag acaagttatt atgaatcaaa
     4441 aggcgatttt tcttttgatg tgcgggatac tcggcttcca agcacgtctc cttggcaact
     4501 gacaggtaca ttgacttctt tatttaagaa tgatcaaggt caagaattaa gcggaacaaa
     4561 attatacttc aatcattctg gatcaaagca attgattcag caaggtcaaa acacgttgat
     4621 ttatgagagt gatggaacag ctaaaggaga ggttctcgtt gactttcccg ataccgatgg
     4681 tttactctta gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt atcaaggcat
     4741 ggtcacatgg gaactgacag ctgggccaac cagctaagtg attagaattt gtatggatct
     4801 gtgataactg tcacagatcc tctttttcaa ggatgagaag gaaacttctg ctgttaaaat
     4861 cagaaatgct tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa aatgaacaac
     4921 ggcaaatcaa acttttccaa cttttattag aggaaaaagg agaggtttcc gttaataacg
     4981 tacttacccg cttatcagtt gatcgggcta ccttgaaaga agatctgttt tatttacaag
     5041 ctaacttgga aaatatcaaa caattggaaa ttgttatcca agaagggaat attttattaa
     5101 tcagacatgg aaatatagca gcagtcgatg tttattatca ttgttacttg cgctattcta
     5161 caaaatatca gatacttctt tatctgttgg aaaatggctc atttgatcgg cagaaacttg
     5221 tcatggaact taatatcagt tcggctactt tgtctcgcag agtgaaggaa ttaaatgatt
     5281 ctttaaatga gtttcatctg aaaataaaaa gcgggcggct gaccggtcca gaaagtcaaa
     5341 ttagttattt ttatttccaa ttattttggt ttggacgtcc ctatcttgaa aacaaaaaag
     5401 aatacagtga tttatcagga aacacttttt tagatctgct gcaaaaagag tttagttttg
     5461 ctattacaga agaaggacga ttgaaatttg aaatatggct ttccattatg aagaagcgaa
     5521 tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct tcctgttgga ttgtttcaaa
     5581 cgacacaatt tttattggac ctccatacat ttttgacccg ttacttcttt caatatgcct
     5641 ttccttggaa tcaaaatgaa acgttggttt tttatctttt catgatcagt aactttacat
     5701 tagatacaca taactcgcat gtccaggagt ttttgataag cgaaaaatac aaagacgacg
     5761 tagtagctca attgaatcga acgtttaaac aatcgataga agaatatttc tctttttatg
     5821 tttttccaga agagatttcg caaaagattg cgttgacagt gatgcaaaat cacgctcatt
     5881 ttgcctattt tagaggatgg atgaatattt ttggccaaga aggattaaaa cagcggttaa
     5941 ctgaagtaga agataaacaa ttcctggttt tatgtgaaag attggtcaga caaacatttg
     6001 ctattgttga ttttactgaa gtagacgagg aaaatgcccg ctttgaatta tttgctcgct
     6061 atgcatctat tttacggacg ctgatcaagc agttagaatt acgtctgcaa attgcttatg
     6121 atcttcctta tgaaaggcta atggcggata tcgtgaaaga aatcatccag gaacaattgc
     6181 tgaatacgat caaatatgat ttagtcgaat acgagaaaga aaagcagtat gatatcattt
     6241 tgactagcca attgaaagag tacccgtcac agaagtccgc taaagttttt gtattcacta
     6301 gcaacgaata taattatgat ttcccctatt tgaaccaatt tttaaaagaa tgttatttag
     6361 aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg atcattggtg cttcccatga
     6421 aggcatcaca gctgccctaa atctaagaaa acttcaaccg aaagcagaag ttttactgat
     6481 cgatgaagat cataaagatg gactaggcta tgtttcaaat ggaatcaacc tttatttaaa
     6541 aggaaagata cgtagtttag ctgaagttgc ccataacatg cggactcttc aagaaagtgg
     6601 ggcaaaactg atcaccgaat ggagagtaac tgaacttgat ccagacaaac atcagctcat
     6661 cctcgcatcc aaagaaggaa aaaacgagac cataacctat gataaattaa tcgtagcgac
     6721 aggttcctct cctgtcacct tgtataaaca aattgaagct gaaaatgtct acacctataa
     6781 aaatctagtg caatctaagc aagtacttgc cgctttaaaa gaagcaaaag aagtagtgat
     6841 cttcggtgct ggttacattg gattggaatt agcagatgct ttgagaaata aaggatacat
     6901 tattcatcta gttgattata tgccgaatgt tttatcaaga tatttcgata aggatatgat
     6961 caacagtttt caaaatcagc tgcaaactaa gcaaataaat ttttatccaa atgaattctt
     7021 gatcgattgg aaaaaatcag aagagaaagt agtatccgta caacttctgt ctcaaacaat
     7081 aaaggcagat atggtcattt tttctgccca aactcgtcct aatacgacat tgttaaaaga
     7141 aaaagtggct ttgtatgagg atgagactgt gatagtcaat gaatatctgc agacaagtga
     7201 tccggacatc tatgcgattg gagatatcgt cccagtcagt tttgacaaaa ataaacggca
     7261 tctcttttta cctttagtta ctcgagctgt acatatggca agagcagtag cgctaacttt
     7321 gagtggtcag ccaaccgctt acgacctcag acaaaagata acagcagcag tcattataga
     7381 ctacttttta ggaactgtag ggctgacaga agatgaagcg ccattcttgg aacaaaatac
     7441 cggttcgtgt tcaggagaat ttgatctatt tccacaatac gacgaagaaa acaaaacagt
     7501 aaatgcaaag ctgatctacc atccggatac attagaaata attggcggac agctgataag
     7561 ccaagaattc ttattatcag atttgaacct gcttgcggat atcgtgaaac acaaaactac
     7621 gattccacag ctagcagttg aggattttgg atttctgtct gaatacacct ctcgttttca
     7681 ctacctcaat gagttagctt ttaaagtact agctaagaga gcaaatagca atagagtaga
     7741 taaaagactg tgagaaatat cacggtcttt tattgtgtgg ggatataata aatattaatc
     7801 aatcgagaat aaaaaattca tataatgaaa tagcggaaga attttttgag cataatattc
     7861 gccaaaaatt tatcaagtga taaaattgtt gtataaaatg attatttcag gaggaggagt
     7921 taaatgtttt tagcattaaa tgaaattatg cattcaaagt tacgttatgc gttagttgca
     7981 ggggtgatgt ttttgatcgc ctacctggta ttttttttga ctgggttagc ttatggctta
     8041 gctcaagata atcgaacagc agtcgacaag tgggaggcag attcgattgt gctttcgaaa
     8101 gatgcaaatt caaatctagg gatgtccatg attactaaga agatagcaga agaagtcgaa
     8161 ggtgggaaag tggcgtatct tgcccaaaca cctggagtcg tgactagtaa agacagtaca
     8221 gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt catcatgccc
     8281 aatcttgttg aaggaaaagc ttttgataac gatgatgagg cagtaggcga tattagtttg
     8341 aaagaagaat atggtctagc cataggagat acagttaagt tatctggtag tgacaagacg
     8401 tttaaactaa ctggctttac ggatcatgcc aaattcaatg tctcaccagt tctctataca
     8461 accatcaatg cttaccaaga aatccgcttc gaaaaagaag atacaagtga aaatgcacgg
     8521 attaacgcaa tagttgtcag aggtaaaatc agtaatttac ctgaagattt agaacagatc
     8581 aaaatttcta aattcatcaa tgaattacct ggatataatg cgcaagtatt gacttttgga
     8641 tttatgatcg gatttttgat cgttattgcg gctattgtga tcgggatttt catttatgta
     8701 ctgacgatgc aaaagattaa tatctttggt gtaatgaaag cacaagggat cacaggagga
     8761 tttattgcgc gttctgtagt ggcacaaaca ttcattttat catttgttgg tattcttctt
     8821 ggtctattag gaacagtggg aacatccctt gttctacctg atgcggttcc attccaaagc
     8881 aattggctgt tttttggcgt cattagcttg ttgatgttag tagtagctgt gttaggcgca
     8941 ctattttctg ttcggacaat cgtaaaaatc gatccgttga aggcaattgg ataagaagga
     9001 ggaagaacat gaaagcaata gaatttaaag aagtagaaaa aagtttccaa gatggcgacc
     9061 aagtagttaa agcattgaaa gaaacaacca tgagtatcga taaaggggaa tttgttgcaa
     9121 tcatcggtcc ttctggttca ggtaaaagta cttttttgac tttggcaggc ggattgcaga
     9181 cgcctagcag tggctctgta tttatcaatg atcaagaatt cagtaatcaa aaagaaagcg
     9241 aacgcgttaa actacgtttt aaagaaattg gctttatttt gcaagcttcc aatctggttc
     9301 cttttctgac agtaaaaaag cagttattat tagtagataa gattaaacat gaacatcgtc
     9361 aaaaagaagc aaacgaatta tttgagcagc ttggcgtgga taagttgatc aacaagtatc
     9421 cagaagaact atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca ttgtacaatg
     9481 atccgtccat tattctagca gatgaaccaa ctgctagttt ggattcagaa aaggcacgag
     9541 aagtagtcga tatcttggca aaagaatcaa aagacaaaaa taaagcaacg atcatggtta
     9601 ctcacgatac acggctgatt gacaaatgcg atagaatatt tatgatggaa gatggtgtat
     9661 tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg tccatcgata caacaaagat
     9721 acacagcaaa aaattttgaa agaagcgact gacttgttta tgtccaaggg atatctcggc
     9781 acttcaacaa gagaaatcgc acaaaaagtt gggatcaccc agcccaatct ctatcattat
     9841 ttcggtgata aagaaaaact atatacggca gttttagaaa accacttaaa agatgttgga
     9901 aaagctttga gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac cttgacaaaa
     9961 atggcgcagt ttttgattga gacacatctg gtcgatttgt ttctgatgct tcacgatctt
    10021 gaaagcaatc tttcaaagga aacaagagat catcttttct ttctatggaa gaaaaattat
    10081 cgtgaacctt ttgaagaaat cttttcagca aaccaatctg tgctaagaaa tgggatttca
    10141 aaagaaatcg cagcaaggca ttttttctta gtgttagcac cttacatcac tcggtcgaaa
    10201 agacctgtgg aagaaccgct aaatgtggcg caattgatcg atttatattt gaatggtgta
    10261 ttaggagaag cgtaaagctg ttatataggg agaaaatagt taggtgaaga agcaagatat
    10321 gacgaagatc gttatatctt gcttctttat ataggtagtt tttcataaat acaaatttgt
    10381 tattctctca tgattatcag ggtatatttt tatttattat gtcggtgttc caggaagtgt
    10441 tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat ttattgggta
    10501 gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc
    10561 attttagcag taatgttatt ttctacggtt atatctttta cagccccttt tttgcttact
    10621 taagctgatt ttaagttttg gcccttagtc tggatctacg aggtgagagt caagaatgaa
    10681 aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt ttcaaataga
    10741 tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca gtacggttac
    10801 aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa tcggaaaaaa
    10861 tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta taaacggcgg
    10921 agagctggcg gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga caaatgatgg
    10981 ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa
    11041 accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg gaaatgatac
    11101 tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa gtacagccat
    11161 aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgaccc atactacaga
    11221 tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt
    11281 tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa tcacgacata
    11341 ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct tcactgatct
    11401 gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag aaaatcttca
    11461 accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa aggaattagt
    11521 aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga ccacttattt
    11581 tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg tttggccggg
    11641 cttattatcg cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt gagcctgccg
    11701 ttcaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt gatgactatt
    11761 gtgacttgtg accgaggagt tggcatgaga acaccgttta agaaaaaggt tgtgaaatca
    11821 acgttttgtt ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    11881 tgttgagcta ataccgagga tcagatgatt gaacaccgtt tattcggaac aagggactgt
    11941 gacatgactt ttgtcacagt tccattattt ttttagtagc ttcaaagctt tttctgtatt
    12001 ggcaaaatga agtttggcat atttttcaag cataggcatg ccgcctttct ccaatagttt
    12061 tgccgctact tggtcagatt tcgttccagc tcctgacgga agggtgattc cagcttcagc
    12121 tgatgctttg tctaactctt ccaaaaagct ggcacgactg atgatagaag cagcagcaac
    12181 agctaaatga tattgttctc ctttagtaac gaagtacagt ttttcttgta attgattttt
    12241 ttctaaacgc gcatattttt taaaatttgc ttccggcgta aactgatcga tcaaaatcgc
    12301 ttctggtttt tctggctcga ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc
    12361 gactttcatc cgaacggcat tgtattttgg ttggatctca ttgtattttt ctggagataa
    12421 gacaagcagt ttatagggga tggttttttt taagatgtca gcaagctgga tgatttggct
    12481 atcttttaac tcttttgaat ctctaacacc aagtttacgt aattctgcaa gctgatcacg
    12541 tttcacataa gcagcacata ctgttagcgg accgaaatag ctgccatttc cgacctcatc
    12601 actacctaaa acggataatt gactgatatt ttttggcaaa gaagaggctg tttttgctgg
    12661 cttggcttgt ttatcctgtg cctcccccca tttatcagct tctattgctg cattcgtacc
    12721 ttgaaacatc acttttccgg atgtataagc ggtaatggtc gtcccgtctt tttttgcagc
    12781 aaagctagta taaggaatct gtttatttaa caaataagag cgatagtatg ctttcatttg
    12841 actaagttgg tcgtttgttg ctttgataat gacgttgctc ataaaattct cctcgttttt
    12901 atacataaaa ctataccgca actcacgact tttcgttttc taagagattg cgagacatag
    12961 cattttttta aagtttatca tataatagtt aagtatgtga aaaatatttg tggagaacgg
    13021 tgaaagaacc aacttcatgg tacactaaat aaaactttca tcaaatagga ggaaatgatt
    13081 cgtatggcgc acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac atatactatt
    13141 atcggacggg aaaccaagca tcatatggat atcgtaacga agctgatcaa tgaacagtta
    13201 gcagaattga aacaattatc accacaaatg gataatgaac aagcagcaat cttgatggct
    13261 gtcaatgcgc tttcggatca attgaagaaa caagaaagaa tattggagct ggaagaagaa
    13321 acagcagaat taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc
    13381 attgaagcaa tcgagaacga agctcgggaa gtattgaaag aaaatggtca agcagatcat
    13441 accattaaaa atcatgtgga agcacaacag attttaaacg agaaacgaaa aggacaaatt
    13501 aaacaaaaat cttctaatta agatcctttt atcaaaataa gaaaaattta aaaaagttag
    13561 aagagaaagg aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt
    13621 ttttctggag cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg
    13681 tcatttattg ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt
    13741 ccatacccag ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt atttgccttt
    13801 cacctagatg aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg
    13861 ctgcttaccc gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga
    13921 caggtagact ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt
    13981 ttgattttgc agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag
    14041 catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag
    14101 ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg
    14161 tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat
    14221 agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa
    14281 gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa
    14341 ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac tgaggatgga
    14401 ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aaattgaaaa tatccgtccc
    14461 catatgaaac ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc
    14521 agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc tgacagtgaa
    14581 atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc
    14641 cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag
    14701 ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat
    14761 gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat
    14821 gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac
    14881 gatcaaagtg cttcaggtca gactttattt attgaaccaa aacaagtggt ggatttgaat
    14941 aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa
    15001 ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatactta tgtgattggt
    15061 aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc catcgtgccg
    15121 ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag
    15181 gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca
    15241 ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg
    15301 gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa
    15361 gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct
    15421 catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt
    15481 gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta
    15541 gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa
    15601 gtttatggat ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca
    15661 ttaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc
    15721 tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat
    15781 gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag
    15841 accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg
    15901 aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa
    15961 aaagcaaatg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt
    16021 cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca
    16081 aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag
    16141 aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc
    16201 caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta
    16261 aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa
    16321 cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt
    16381 cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt
    16441 ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag
    16501 ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca
    16561 aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc
    16621 agatggttcg ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg
    16681 tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac
    16741 ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt
    16801 tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg
    16861 atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga
    16921 aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc
    16981 aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa
    17041 tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt
    17101 gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta
    17161 attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt
    17221 cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc
    17281 aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa
    17341 acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc
    17401 gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg
    17461 ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg
    17521 gtgattactc gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc
    17581 ggtgcggcga atgagacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa
    17641 cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgcct gtgtccttat
    17701 tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta
    17761 aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa
    17821 aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc
    17881 atgacttgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg
    17941 attgataaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa
    18001 cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc
    18061 ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg
    18121 atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga
    18181 gaaaagaaaa aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg
    18241 tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac
    18301 gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg
    18361 acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat
    18421 cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc
    18481 atctatcgca gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg
    18541 atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga
    18601 atcgatattc ctattgctgg catggtaaaa aacgataaac ataaaacaaa tgagttgcta
    18661 ttcggtacag atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc
    18721 cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt
    18781 aaaaacagtt ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa
    18841 atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta
    18901 caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa
    18961 tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa
    19021 gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat
    19081 gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt
    19141 gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg
    19201 ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg
    19261 acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat
    19321 gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa
    19381 ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac
    19441 ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct
    19501 aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc
    19561 attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga
    19621 atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat
    19681 gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga
    19741 aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta
    19801 agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat
    19861 acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact
    19921 cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa
    19981 ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa
    20041 aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat
    20101 catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt
    20161 ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt
    20221 caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc
    20281 gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc
    20341 attaggacaa agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct
    20401 tcgagcgagt caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag
    20461 tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa
    20521 ctcgacagtc caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt
    20581 gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga
    20641 agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt
    20701 acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca
    20761 attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat
    20821 tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt
    20881 tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt
    20941 gccagtgact tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga
    21001 tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat
    21061 gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt
    21121 acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt
    21181 actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag
    21241 gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt
    21301 gttcccagct tctttttgta tagacctatt ttatttattt tactatattg gatatatatt
    21361 cattgaaaat agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt
    21421 taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca
    21481 cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga
    21541 gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc
    21601 ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat
    21661 tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag
    21721 ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata
    21781 cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct
    21841 tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac
    21901 ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat
    21961 ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca
    22021 aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata
    22081 ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg
    22141 cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat
    22201 tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta
    22261 aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc
    22321 ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc
    22381 gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct
    22441 atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg
    22501 aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc
    22561 aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg
    22621 tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag
    22681 accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg
    22741 caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg
    22801 ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca
    22861 cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt
    22921 ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta
    22981 atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag
    23041 tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt
    23101 attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta
    23161 ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc
    23221 aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta
    23281 cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat
    23341 tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac
    23401 ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg
    23461 ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat
    23521 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa
    23581 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc
    23641 agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta
    23701 cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc
    23761 tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa
    23821 gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc
    23881 ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct
    23941 gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt
    24001 ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt
    24061 tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta
    24121 gctttggatg aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa
    24181 taagtttgtc gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa
    24241 taagaggcaa tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca
    24301 cttgatttcc ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga
    24361 ttggtttcat ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaaatt
    24421 aaactatgta agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga
    24481 gaaatttcgt gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa
    24541 aagtaagcag aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg
    24601 agaaggacat tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt
    24661 tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt
    24721 agaagctttg gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact
    24781 gcggcagcag ctgaagatct atgcaacaga aaaagagcag gaattggaaa cgatccaaac
    24841 aaactatcga caacaactaa aacagttggt ggaagaaaaa gaacaatttg aaatacaaga
    24901 attagaaaaa gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa
    24961 agatcaatat caaaagcatc agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga
    25021 aacttatggc cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg
    25081 agaggatttt acaagcagta caaggaatcc aaaacgttga aatccgggat ctgtatcaaa
    25141 atagaacaaa caaccagtgg gtggagaagt attttccaga taccacgatt attgataaag
    25201 aagcaaaatt aagcgcttta ggtaatcggt tgaaagacat ccaagaagct atccaattca
    25261 tacaacacca cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct
    25321 tacaagattt ggaagccaat tattccgaag aaaatttcat aaataaattg aaagaagtcc
    25381 ttgatttaaa agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta gaagaagagg
    25441 aagaatggct tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga
    25501 cgaccatttt tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac
    25561 tgagtaagct tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc
    25621 tttcgtatgt tgctttaaaa aaatcagttg ctgttataga agaaattgct gggaaatacg
    25681 ggttcaataa agaaacgtat ccttatgaac aaaatcctaa agaaaaactg caagagacca
    25741 agcagcaatt atccattgtt cacgagcagc aaaagaagat aacgacagcg ttaggtcagt
    25801 gcagtggcta tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac
    25861 gagaaaaaat aaaagaacgg tttatccatg cgacttatct gatcgttttg caaggttggg
    25921 tagatgcaga agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag
    25981 atatatacgt cagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta
    26041 aattgaaaaa ccatccgctt gtcgaacctt ttgaattgct gactgaaatg tacagtctgc
    26101 caaaatatga agaagtagat ccaacacctt ggatgacacc attttatctt gtcttcttcg
    26161 ggatgatggt cgcagatgtt ggttatggtt tgctgatgct cattgggaca atactagctc
    26221 aaaaactact tgttctacca cgcgggatga aacgtttcgt aaaattcttt gagattctct
    26281 cgataccatc gatcatctgg ggattgatct acagttcatt tttcggacag tcattaccaa
    26341 aagaactgtt tggcatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata
    26401 cgattttgat tttatccgtg attttcggtc tgatacagat tttggttgga ttatttgtgg
    26461 cagcaaagga aaatatcagg agaaaagatt acttgagtgc tgtcagtgat ggatttgcct
    26521 ggcaagggat cctaattggg atcgttattg cgttggttgg tgccatgttg ataaaaaata
    26581 cagcatttgt ttaccttggc gctgggctat cgatcattgc tgccttatgt atcttgatcg
    26641 ttccgattat ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg tataatcttt
    26701 acggactgac cggctatatc ggtgatttag tcagttatac acgtttgatg gcattgggta
    26761 tttcaggcgg aagtattgga gcggcattca atatgctagt tgctttcatg ccgccagctg
    26821 ctcgattcag tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga
    26881 cactgctaag tgcctatgtc catggcgcac gattgcagta tgtcgaattt tttgggaagt
    26941 tttatacagg aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca
    27001 tcaatcacaa gaaaaaaaac aataaataat tggaggattt ttatataatg atggattact
    27061 taattagtca aaatggtgga atggtatttg cggtattagc gatggcaaca gcgacgattt
    27121 tttcagggat cggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcattga
    27181 caacaagtca acccgaaaaa ttcgggcagg ctctgattct gcaattgctt ccaggcacgc
    27241 aaggtttgta tggattcgtt attgcctttt tgatttttat caacttagga aatgacatgt
    27301 cagttgtaca aggactgaac tttctaggtg cttctctgcc gattgctttt acaggcttgt
    27361 tttcaggtat tgcccaagga aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac
    27421 ctgaacatgc tacaaaagga atcatttttg ctgcgatggt tgaaacatac gcgattttag
    27481 gtttcgttat ttccttccta ttagtgttga atgcctaagc ataaaggagg aaaagcagat
    27541 gaacgccatt gaaaaaatca tctctcagat gaacgaagca gcagagcagg aaagagcggc
    27601 gttagaacaa gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt
    27661 tgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg
    27721 tcagctaagg aatcgccagc aagtagaagt acgacaagaa aatttgaatg cgaagcagga
    27781 atttttaaga cgattgtttg cagatgcggt aactgaaatg gaaaattggg atgaatctga
    27841 acaaatccag tttatcaaaa atgcattgta ttcattacct ttgacaggaa aagtagcatt
    27901 cattgctgga gaaaaatcag cagcctatct ttctcagacg ttgttggatg aatggaacaa
    27961 tgaacttccc tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct
    28021 gataaacgat caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca
    28081 aggaacgatg agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg
    28141 gaataccacg aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag
    28201 acatttgaac ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc
    28261 acgatttaca gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta
    28321 aaggagcaga gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt
    28381 tggatctata cgatgcgtta tactttccat aatctgaagg ttttgacaaa agctgagatg
    28441 acaaatcaaa atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg
    28501 aaggatgcca ttcatacgca agtttcaaat gaacttcctg aaagcattat ggacagtata
    28561 cgagaagtac gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat
    28621 gatcgacagt ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg
    28681 ctgacagaag tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata
    28741 ttgcagaaaa gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa
    28801 aaagaagtat ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta
    28861 gagacagatt atgctgattt tctgcgccct gtcatccgtg agggaaatat tgatctcgta
    28921 aagcttgaac agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag
    28981 gctttcggac cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac
    29041 cttcgtttac tggttatcgg caaaagaagc catttcgaat cagaaacaat cagaaagcgg
    29101 gtgagacaag tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc
    29161 attcaaattg tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc
    29221 aattgaagaa atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc
    29281 attgagccca gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat
    29341 tcccaatcat caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa
    29401 agcagtaggt caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa
    29461 aatcataaaa gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca
    29521 agacatgtgt ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga
    29581 tgtggcgtct attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg
    29641 ctctactggt gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga
    29701 cggaatccaa cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg
    29761 cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga
    29821 agccggagaa acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat
    29881 aaaacataaa atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg
    29941 ttcattcact attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt
    30001 gacgatgctt caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc
    30061 ggatgcaccc atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg
    30121 aggagcagca gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat
    30181 tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga
    30241 gatgacggat gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt
    30301 gatggaacgt acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc
    30361 ttctatctat acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc
    30421 tatcatggca gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt
    30481 ggaagaaatg cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata
    30541 ctatgaacga tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac
    30601 cgcaatcagt gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac
    30661 attgcgggtc gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt
    30721 tccttcgatc gattggatac aaagctattc tctttattca acagaagtag ggaagtatat
    30781 ggatcatatc cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca
    30841 ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa
    30901 tgaccgtctg acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc
    30961 ttttgatgat gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat
    31021 cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat
    31081 catggacggt accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac
    31141 agaattagaa aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt
    31201 ttcagaagga ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc
    31261 cattgatggc ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta
    31321 tgcaaaatgg cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg
    31381 tccaaatatt cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag
    31441 gccatccttt agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg
    31501 gacgaccgaa agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag
    31561 aagtaatcaa tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg
    31621 caatcgatca tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg
    31681 gtcttcctca taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat
    31741 cagatgattt cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt
    31801 tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag
    31861 caaacgaccc tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat
    31921 atcttgctta tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg
    31981 cagaggcatt gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc
    32041 ctgggtatct ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac
    32101 taaaaggatc ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc
    32161 caatccctga cttgactggt tatattacag aaggacagat catcttgact agagagcttt
    32221 acaagagtgg gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata
    32281 agggaactgg acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg
    32341 cagcctatgc acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt
    32401 tatctgatgt agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca
    32461 atcaaggatt ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc
    32521 tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc
    32581 tgccagaagg gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat
    32641 tgactcgatt gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata
    32701 agcaagatga actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc
    32761 aagaaatgga agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga
    32821 tcacggaacc tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta
    32881 tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac
    32941 agctgacaga cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt
    33001 caatcgatcg gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa
    33061 cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat
    33121 atatgacgat ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa
    33181 acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag
    33241 tggtatcttt ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg
    33301 tttgccggct gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg
    33361 cctacttacc ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct
    33421 tttcactgca acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca
    33481 ttggaacagt gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt
    33541 tatgatggtt ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg
    33601 tattatatta aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct
    33661 ttacatccta aaattggcct ttgtgatcca attgattggt acggttgctt tatcttttgt
    33721 atttgtgcct gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc
    33781 tagtttttgt aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa
    33841 tgtttatttg atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt
    33901 atggcgtgat ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc
    33961 attaaccgtt acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg
    34021 caatggggca acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat
    34081 gagtgtgaca cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc
    34141 aggtctgatt ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg
    34201 cggattgaaa acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg
    34261 acgtacgaga gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact
    34321 tactttattt tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac
    34381 tgaaacgata cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt
    34441 tggtacagta ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat
    34501 cattatagcg cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat
    34561 gacaaaagca caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg
    34621 ataatttcat cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt
    34681 ttcttgcttc ttggatactt taagtgcaaa atgttcgttt attttttgag gcactacccg
    34741 ttgttgaacc tcgggtagtg cctctttttt attgaaagga atcaataaga tttgattcct
    34801 ttatttatca ataggttata attcatattt ttttattttt tcttttacga tacttagtcc
    34861 agaagctaaa atttcaaggt tattagagga tagggaaatt cgaacgaatg catctggttg
    34921 cgcacgtgta gtaaagcgtc tggaatgata aagtcggata ccgttagcaa gaaagtcatt
    34981 ctcaattatc ttagggtcaa tctcaggatc aactggaaga attcgatagt aagggacacg
    35041 attcgttggt ttttctactt tgaaaagaga atcaaaaagc tgattggctc tttttgtaag
    35101 acttaattta gtttctaaga tcctatttgc ttcatttgaa tggaggattt gagtgatgac
    35161 ttccacatct aatccagaag ttttcacatt tatattaaaa attgcttgtt ctatcttgct
    35221 ttggacatgt actggaacta caagataagc aactcgcaat ccagaacaaa taaattttgt
    35281 cattccagct aagtagattg tttggttagg taataaattt tggaaaggag gaatgatcgt
    35341 atgttgatag taagttgtta aaaatgaatg gatatcatct tcaataaccc atagaccttc
    35401 tttttcaatc acttcggcta attgctctcg tcttttgtga gagatttgaa accctatagg
    35461 attattacaa gaaggcataa gaaagacacc atgaatcttc ttttttctac attcctgaat
    35521 caatatatca ggtcgcattc cttgctcgtc aaaatcaatt ggaacgatct ctaaatgata
    35581 taactgagcc aattcaatga aattagcgta tgtatatcgg tctgttgcta ttctatcacc
    35641 tggagagaaa agtgcagaaa gtgtgatagc caatccattt tgaacaccgg aaacaataga
    35701 tagagattct aaggacggaa tgactccttg agtttttaac caatttgctg cagtagtcaa
    35761 ttgaaaattt gtgcctttgg gatcttggta attaagcaaa ccaacaagat ttgggttatt
    35821 actagttgat tgaataaacg ggacaacgaa tttattacat tcttcaaaag aactaaccag
    35881 accaaaatca atgatatgtt cggttacttc attggtagag atggtgatgg actcaaagac
    35941 attgggagaa ataaatgtgc cacttccaat attcgtatac agcaaacctt tctcaattcc
    36001 atatttataa gccttgccaa cggtagtaaa atttaaatct aaataatcag ctagctctct
    36061 ttgggagggt aactgagtgt ttttctgtaa tttattcttt aaaatatctt gttctaattg
    36121 ttgaatcaac gatcgataat aaggacgctt caatttttgt ttgtctggtc gccaagttaa
    36181 cgggtaattg tcataagaat taataggcat tttctccact ccttaaaatt gtataccata
    36241 caattttacc attgattgta ttgattttag aagttaatct tttgtgagga gggaattgtc
    36301 tatgaaaatt attaattttg aggaagtagt agcttctttt agttttgaag aagcgattca
    36361 agtaatgaaa aaatgttttt ttgattatga aagtggtaaa atcagccaaa atcctcgatt
    36421 ggtggaaatt ttacctgacg gaaaaggcaa aaatatgttt gcgatgatgc ctgcttattt
    36481 gggggagaac agatattttg gcacaaaagt cattaccgct tttccggata atcatcagta
    36541 tcatttacct tctcatttag gagagatttt cttgtttgat gctcaaaacg gactacccaa
    36601 agcattgata aacgcaaatg cagtcacctg gattcgaaca gctgctgtat ctgcgctggc
    36661 aacagattac ttagcaaatc cgcatgcaga aacacttgct ttaattggag ctggtcaaca
    36721 agcattgtcg catttgactg cgatttctac cattcgtccc atcactacag tgtttgttta
    36781 cgatatttta gaagaaagac gaaatacttt tatcgctaaa gctaaaaaac agtatcctaa
    36841 aataacgttt ataaatgcaa tatcagtaga agatgcagta cgtccagcgg aaatcatttg
    36901 tacgttgacc tctagtaaag aggcatttct tcaaaaagag tgggtaaggg aagacgcaca
    36961 tatcaatgct gtgggcacat ttactcctga tacaagagag ataagtacat cgttgatgga
    37021 aacttctcaa atctatgtag atgattactc agctgcctta acagaaagcg gggacctgct
    37081 tatagccatg gcagaaggag atttagagga atcttgtatt gttggtacat taggcgaatt
    37141 agtttctgag aaaaaaacat tttataaagg agaatcgagg ccgacgattt tcgacgcagt
    37201 tggtctagct attgaagatc tttgttgtgc agaatatatt tataacaaat tagagaaagg
    37261 ggctttaaaa tgaacttaga ttattacgta gttgatgcat ttgcagatga ggtgttcaaa
    37321 ggaaatccag cagcggttta tgtgctagaa gaatggctgc cagaaggtac gatgcaaaaa
    37381 attgccattg aaaacaattt atctgaaaca gcatttacag tgaagaaaaa ccaagaattt
    37441 gagcttagat ggtttacgcc agatcgagaa atcgatcttt gtggtcacgc gacattagca
    37501 acagcatttg ttttatttaa ctattacaaa atacctgatg aaacgattaa attttctact
    37561 caaagcggta atctatttgt gacaagaatt aaagattatt attacatgga ttttcctagt
    37621 attatgccga aaaaagtgcc gatattgtct gaatatgaag aagcgattgg ggctaagatt
    37681 aaagaagcct acttagcacg tgatttattt ttcgtattag aagatgaaaa aacggtggct
    37741 aaattgcagc cagattttac agctatcaaa aattttgaat taggaatagg agtcatcgtg
    37801 acagcagaaa gtgtgcaaaa agactttgtt tctagaacct tctttccaaa attaacaatc
    37861 aatgaagatc cagtatgtgg atcagcacat tctaatctta ttccttattg ggcagaaaga
    37921 atgaataaaa caaaattgtc agcttatcaa atgtcatcaa ggggcgggga tctagaatgt
    37981 gaattaaaag aagatcgtgt gataatagga ggaaaagcag ttttgttttc taaaggtacc
    38041 tcgtttattt gatttctttc aaatgaatta aaaataaatt ggtggagagt ggcaacctta
    38101 tagctacaat ggttgttaaa aaaagacgca aactttttca atttaatgaa aagtttgcgt
    38161 ctcattttaa ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc cccatactaa
    38221 ctatcctcgg atgacttcga ttttataacc atcagggtca atgacaaagt agtaagaagg
    38281 cgcagtgcca ggtaatcctt ttaagtcagt aatctcaaga ccagcagctt tatgttgctc
    38341 gtgtaatttt tctagatcat cagctgctat agcgatatga ccatagccgt ctcctagatc
    38401 atacgcacca tgatcatagt tgtaagtcaa ctctaattca tagtcatccc caggaagtgt
    38461 caaataaaca agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt cttcaaaacc
    38521 aaatgccttt tgataaaatt cgattgatgc ttcaagatct ttcactcgaa cacatgtgtg
    38581 agccattttc atagattcat ctctcctttt ccttttactc aatcataaca aaaagaagta
    38641 aggaaatagt tgatttgtgc ttgttcatga gaaatttctc atgtaaaatg gatagaaaat
    38701 aaaactgtta aggtggaaac tattcatgga taaaccaatt tttggtgaaa aaaagcaatt
    38761 tgctgattat caaacccgtt atgcagcata tattgtcata gaaagagatc agcagattgc
    38821 agtaatcgag gctccaaatg gtgctttttt tcttcctggc ggggaaattg aaggagaaga
    38881 gacgaaagaa gaagcgattg tccgtgagct gatagaagaa atgggaattg tggcagaaat
    38941 ttcttcttat ctgggagaag ctgatgagta tttttactcg aattatcgtg caacttatta
    39001 ttataatcca ggatatttct ttgttgctga ttcttggaag cgtataggag aaccaactga
    39061 aaagaccaat aaaatttggt gggtgagccc tcaagaagcg cttgtgaaat tgaaacgtgg
    39121 tagccaccga tgggcagtcg agagatggat cgaaaaaaat aaaaactgaa agaaagcgag
    39181 tagatcagct ttctctcagt ttttttattt attacagtac atattttttg attgcttcag
    39241 ctacaccgtg ctcgtcgttt gatgaagtga tgacatttgc tgcttctttg acaagagaaa
    39301 cggcatttcc cattgctact cctagaccag catattcaat catggataag tcgttctcgt
    39361 tatctccgat agccatgatt tcatcttgat caataccaag gtgctgtgcg agattcgcaa
    39421 ctgccgcacc tttacttgct tctttattta gtacttcgaa gtaatagggc gcacttttga
    39481 ccgttgtgta tttatcacgg aaagtttgcg gtaaacgcgc aattgctgca tctaaaattt
    39541 ctggttcgtc gatcatcatc attttgacga tgttcatgtc tggtgtcatt tcttcaggtg
    39601 ttcgatattt tagcggcatc ttgactaaac ttgcttcgtg gatcgtataa gcgctgatat
    39661 ctcgattagg cgtgtagatc gctttgtcat cgatcgaatg gagatgactg ccgacacggc
    39721 gtgccattac ttcgatctct aggaaatctt catgtgtcag tccgtgacgg gcgatgattt
    39781 tacctgattt cgtttgttgt acgagtgccc cgttataagt aataacgtaa tcattatctt
    39841 gaaaaaggtt caactcgttt aactgttcct ggacacctgg taagggacgg ccagtgcata
    39901 aaacgatcga aacacctttt gcaacagcag cggcaatcgc ttcttttact tcttttgtta
    39961 cttctctttt ctcatttaat aaagtaccat cgatgtcgat agcgactagt ttgatagaca
    40021 taatttcctc caatatattt taatttattt caatcaatgc gccattacgg atatggctgg
    40081 caaattcttg ataagtttcg tcaaataaat cataatgatc acgcaaggac ggatcaacca
    40141 tttcttttgg aaagaaaaat cgctcatctc cttgttcttg tcccgctaat gcagcaacaa
    40201 gctgactaac ctgtgataat tctatcaaag ttccgtcatt tcgcaaaatc tcgatttgtg
    40261 tgcgatgtct gccttgttct gggcgataaa aatcataagg tagatcgtag ctagaattca
    40321 cagcagtgta ataaacagga ttatatccga ccttttctac taacaaagtg agttcttgga
    40381 ctaattttga atcccggttg ccagaaaaag tagcagattt cagtggttta cgattcaaaa
    40441 aacgtttagc taaatcattt aaaataggat cagcagattc agaccattga gtaaagtaag
    40501 tgcccaagac cccatcatca agtttgaggt attcttctaa agtaaagctt ccgtcaagaa
    40561 atggcagaag taattgagaa tggagttcaa aactgctggc atcttcttga tacaactcgt
    40621 gtgcgcgatg aagcagatgc tccaaaatta cttccatacc acgggaaaca ggatggaagt
    40681 acacttgcac atacatttga tatcggctaa cgatataatc ttccactgca tgcatgccac
    40741 tcatggaaaa agcgatcccg ccttcataag gacgaatcac acgtaatatt cgggtaagat
    40801 caaatgttcc atattctgtt ccagtgaaat aagcatcccg aagcaaatag tccattcgat
    40861 ctgcatcgat ctgactagaa atcatttgga caacttgtgg gttggggtaa gttttttgga
    40921 tcacactagc taccttttca ggaaaacctt cttctactcg gttaagaatc tgatacactt
    40981 ctgtttctgg agaagtgatg atctctacag tgatggcttc atgattcgta tggaagatat
    41041 gttcaaatgt atgagagtaa ggaccatgac cgacatcatg caacagtgcc gcacataaag
    41101 tcaccagccg ttcctgatca tcccagcctc cgttgcctat tttttctttt gaaaaatttc
    41161 gagaaaagat atcgcagatt cgtcttgaaa tctcataaac gcctaatgaa tgagtaaaac
    41221 ggctatgttc tgctccgtga aaagtgaaag aagaagtgcc taattgttta atgcgtcgta
    41281 gacgttggac ttctttcgaa ttgatcaaat ctaagattac ttgatgctgg acatggatat
    41341 agttatggac tggatcacga aaaacttttt ccataggcag catttgatat ttataagggg
    41401 ttgtcataat gtagctcctt tatgttatta cgttgttcca ttctctggat ttggttgttc
    41461 cattgctcca gctggatttc tttttttagc cagtcggtag aatcctgtaa atcacttgca
    41521 gaatacagct tttcaaaagc ttttgcaaat gcttctttga cttcttttac aaaaaggggt
    41581 tggtcaagca gttcttcaag tgttgccatt gtagcaggat ccactgcagg atagccattt
    41641 gtaccaaagt cttcttttaa agcagattga taaaatcgct tcatgacttg cccacgtttt
    41701 tcctgattac cgttgacact taagtacatc ataacagaaa tcccattttt gatccgacgc
    41761 tgagcgatac cggcaaattt tttgccatgg atgcttaaat cataagttcc aggacaataa
    41821 gaatgggtga tttcgaatgc ttgtatcggt gcctttggaa aagcgtattt agtcaatagg
    41881 agcatttgct cgtaagcgtc atctatggtt attttttttg tgtcactttg ggggaaaatc
    41941 aaagaaatat tcaaaattcc ttcatctgat acgatcccta atccacctga atttctgatc
    42001 actggagcgt aacctgcatc ttccagtgtt tggatcccgt cacttagaaa aggagttcga
    42061 acatccttca tacctaagat gactgtgcag tcatattgcc agaaatgtaa aaagatttgc
    42121 tggtgttctt tagaatattc cgttaatata tctgttaatg caaaagggga aaagtcgttt
    42181 ttttgataaa tgtgctgatc tagtcgaatc gcagggttca ttcaaccggc tcctttgtat
    42241 aaattcatat tttctacaat attatactct aaaatcaaag agttgttttg gaaaaaaggt
    42301 tttctgtaaa aatggacaag ttggttgaca aaacaaaaga aactaggaac aatgtagcta
    42361 tgagaagtaa aggagaatga ataatggaat tatcacaagg aacaccgatt tcaatcaaat
    42421 tgcgtacaaa agtaaaacag caaggggaag tgcaagattt ttattttgat ttgaaagggc
    42481 aaatggtgac aattggcgat acgctgtaca tacgttataa agagattcag gaggagacgg
    42541 gcgatgagat tcctgtaacg atcaaattag ttccagatgg tcatgtacag ctgatccgtg
    42601 caggagaaat gcggatgcgt ttgaaatttg gctataaaga acggttagaa acatcctatc
    42661 gaacaccata cggtatgatc cagattgcta cttttacgaa agaactgcat gttagtttga
    42721 aagaccgtcc cacagcaggg aaggttcgaa tcgattatga tctgttcatg ggaccggaaa
    42781 aaatcggcga atattatttg accttggatt ttacagcgta aatataaaag aaaagtttga
    42841 ttttttgtgt ctgattttgt atgataagga gtagactgtg aaaggacgtg tcctgattgg
    42901 aaattagtgt atttgaaggt gcaaacaaaa gcgaattatc aatgatcgaa gtagcacacg
    42961 cgatcttaga acaacgtggc gatgtcatgg atttttctga tttagctaac cagatccaaa
    43021 actatcttgg gaaatcagac agtgaaattc gtgattcact cgcacaattt tatactgact
    43081 tgaatattga cggcagcttt atctcattag gggataatcg ttggggcttg cgttcatggt
    43141 atgctatcga ttcgatcgat gaagaagtaa accatggaat ggacgaagag gatgaagata
    43201 ctccacgtcg ccgtaaacgc aagaaagtca acgcctttat caatgatgat gaagacgcga
    43261 tcgattacaa tgacgatgac ccagaagata cagatctgac agaagaagat gacgatgatc
    43321 tgtttgatga cgatgatgat gaagatgaag aaatcgctgc ttataattct gacttgcaag
    43381 aaatcggagc agatgacccg acagatgatg aagaagactt gccaggaaat atcgaagaag
    43441 atttaagtat catagacgaa gacgatgatg atgaagatta tgaagaggaa gaattttctg
    43501 actctgaaaa agaataaaca ttattttctt acgagaggtt gtgacagaga cgatcagcct
    43561 atcagaataa ggaggtgtga cacgattttt gtcacacctt cttttttgta tataaaaatc
    43621 ccaagtaaat acataaaaaa taaaaaaatt gaaactttta gcgcaccttg tacgtcatat
    43681 taagtgaggc agaaaggagt ggagaatgat ttttactgga tataagaaaa aacggcagat
    43741 cgaaaaatac cgtcacctgc tgaatggact catcgaaact gattatcaaa aaatgttccg
    43801 tatcacgatg aattacgtac atgataaaga agaggcctta gatgtgatgc aagatagttt
    43861 tcataaagcc ctgaactatt ttgaaaaaga aaaagagatc gagcatttta gtgcatggtt
    43921 ttatcggata ttgatccgca cggcattaga tagttggcgc agacaaaaaa ggacgcctgt
    43981 ggacttattt gaaattcaag atatccccca acagttacct ttagaaactt ccgttgcaga
    44041 gctccatatg attttagata aaatagacag tccggaaaaa gagattctga tcctctactt
    44101 ttttgaagga tttcgtttga aagaaattgc gactatcttg gatatgaatg aaaacacagt
    44161 taaaacgaaa atgtaccgct cattgaatgc tttacgtcaa atcctagaat gaaggagaga
    44221 agagtgccat gtattcccaa aaagaaaatt taaaaaaact aaagaaaact tataaagaac
    44281 aagcaattcc caaagggctt aaagaggaga tccgccagcg ttttattatc gaagagcaat
    44341 catttataaa agcaaagcgg agaaagagac gtctccaagg gttcggagta tctttggcta
    44401 tcgtaatgat tacaacaagt agccttcttt ttaatagtca agttcgaagc tttgcagaag
    44461 atttacccat cttaggatca gtcattgaat tgatcttagg cgaacgcttt actgatcgat
    44521 ctgaaaaaat cgatattcag gtaccgaaga tcaatacaca aaatgagaaa gaaaataaaa
    44581 caattcatgg attaaatcaa aaatactttc gcgaaggaca agcagatttt gaaaaagctg
    44641 aaaaagaata tggaaatttt gaaacggatc attaccaagt tttgggagat tatcagaaaa
    44701 tcgtagacga taaccggttt ctcgtgattg aaaggcagat cacccaaaca gcagcagata
    44761 gccatgttga aaaaagatac gacacaatcg acaagaaaaa cagtgtccag ctctcacttc
    44821 cacttttatt caaagatgac acctatctat ctgttttgac aaaagaagtc aaacggcaga
    44881 tggccgaaca agtcaaggaa gatccatcta agtactattg gactgagcaa gatattcaag
    44941 aagggacgat agaaaaacca acacttgtta cgcctacacg aagtttttat ttgaacaaag
    45001 agcatcaatt agttttgact ttttctcaat acgaaattgc tcctggttat atgggtacac
    45061 cagaatttgt cattcctaag tccgtgacaa aaacgatttt ggcatcggaa gattatttgg
    45121 atcattagac gagaagtagt tgcgactagc agtttcaatt ttcaagtaag tacaagtaaa
    45181 taataagaag agaaaaagag aaagaagaga tatagtgaaa agaaattatt tagttatcag
    45241 tattattgct ttatccgtgc ttttgttttt cgaaattact tcggtggcct ccttgatcac
    45301 cgaagtaaaa ggacaaacac actcttcgga gcattcgtca caatctttca aacaatcaga
    45361 acaatcaacc attaaaagcc aatcaactga aacagctaac gtcaaagaag ccattcaaga
    45421 atcagcggaa aaggtacaaa gtcagtcatc acaatcaaca caagacagta caaaaacgag
    45481 ccgagatccg gctgtcaaaa gcgtcgcaat cagctttgat gatggaccag gagcgaccac
    45541 aacaccgcag cttctgcgaa tcttaaaaga aaaaaacgtg catgccactt ttttcgtctt
    45601 aggagaaaat acggcgcagc atcctgaaat cgtgaagcag acagctgaag cagggcacga
    45661 gattggcaat cacacatacg atcatcagaa tctggccact ctttctgccc agtcgatgac
    45721 ggaggaagtg acgaaagcag acactgagat caaaaaagtc actggcaaaa cccccacttt
    45781 tgttcgtccg ccctatggtt ctgtcacgaa tgtaggagca acgatcatcc agcgaccgat
    45841 catcgaatgg tctgttgatt cagaagattg gaagacaaga aatcctgatt tgattttgca
    45901 gaagatccaa gcaacagtat acgatggggc aattatttta ttccatgata tctatcctga
    45961 aacgattcgt gccgttcctc aagtgatcga ttatctgaaa gagcaagggt atcgcatcac
    46021 gacagttggg gatttattag ggcatccaac ggctgtcgag aactattatg gcagaaacga
    46081 tcatcggcca gttcagtaag tgagacaagt atctttaata aataacacat tttaataagt
    46141 agttgaacac ataactacct tacttgcaaa ggcttaacaa tatctataca ggtacaacaa
    46201 aaaactgtcc accaattttc tttggcggac agttttcact taaaaataaa gaagcaacta
    46261 ttcgtaaatt atgctaaaat agcagtagaa cgcgtcctga gggaatcgaa cccccgtctc
    46321 aagaaccgga atcttacgtg atatccacta cactaaggac gcaagcactg ggataattgt
    46381 aactaaacag ataaaaaatt acaaggggat tggacaaaaa aatgaaattt gagcaaaatt
    46441 tttcacaaaa acagcagcag acacagaaac tagccatgac gcaacagttg caacagtcca
    46501 tccaagtttt acaatataac agtgaggagc tgttggcatt cgttgagaat caagcaatgg
    46561 aaaatccatt ggtggaagtt gttgagccgg aatggcagcc agattacata aaagcttctt
    46621 catcttctta tgaaggagaa gagacaaatt acctaaatca gattcctgat acaaaagggt
    46681 ctttatttga ttcattgatc gaacaagtcc atttgaatta ccgagatact tttttgagga
    46741 aaatcgttct ttatcttgta gaatacatcg atttgaacgg atttttgacg attagtttag
    46801 aagaagccgg aaaagagatt ggagctacgc ctatccaaat gctcgatgcg ttgactctga
    46861 ttcagcaatt agaaccagct ggagtaggtg cacgtagttt gcaagaatgt ctcatgcttc
    46921 aaacagaacg agatgattat gcgccggaat tagcttatat cgtgttggaa gaatgttttg
    46981 aggaactggt ggagcgaaaa tggaaagaaa tcgcacaacg tttttctgtt gatctacatg
    47041 ctgttcagca gatattcgac tatttacaaa cgttgagtcc aagccctggc cggattttcg
    47101 accgtagcag cgaattgtac attattcctg atgtccgggt attagtggac gacgataaaa
    47161 atgtacaagt tatctcaaac cgaaaaaatc agccaaatat acgctttcaa gagagttact
    47221 tcaaacaaat gtcacaacaa gcagataaag aaactgaaaa ttatttaaaa gaacggaaac
    47281 aggaattcga atggttgaag aaaacgatcc tacaacgtgg ggatacaatt ttgcgggttg
    47341 ctcaagtcat tgtttcccgg caaaaagctt tctttatcga caaggaacgt ccaatcaagc
    47401 ctcttacgtt aaaggaagtt gcaactgaga tagatgtgca tgaatccacg gtcagtcgtg
    47461 cagtcaacgg gaaatatttg gaaacagact tcggtgtatt tgaattaaaa aaatttttca
    47521 ctacacgaat tcctacgaat agtacagaac agacagaaga cctttcagca gatacagcga
    47581 agaaaaagct gcaggaatta gtcgatcaag aagacaaaaa caagccgttg tctgaccaga
    47641 aattggttga attgctgaaa aaagatgaga tagcgatctc aagacgaaca gtagcaaaat
    47701 atcgtgattt attaggaatc ccgagttcat caaaaagaaa acgttatgat aactaatcag
    47761 tcaaaagact agaaaaagta tttcactttt tctagtcttt ttagttgaaa aacgagagca
    47821 aaactgttag acttacagtc gtgaggttga gatttccctt tattgatgtg aaattcgaat
    47881 agttaatttc tttttttatc gtatatgggt cgttttaagt ctacattgga cgtaaagtgt
    47941 ccaactaagg agatcgctat gctggatgaa ataaaaatga ttgaatctgt tgcaccagat
    48001 attatcgaag tgttgcaaga acgctataaa attttacgaa atatctattg gatgcagccg
    48061 attggacgtc gaagtttatc ggaaagtatg gggatcacag aacgagtatt gcgaacagaa
    48121 acagatcttt tgaaaaatct gaatttgatc gacacctcaa aaagcggtat gacgctgaca
    48181 actaagggcg aagaagttta tcaaagcctt gaaaacttca tggatcagct tttaggtacg
    48241 catcaaaccg aacaacagtt ggcagaatat tttggtaacc aacgctgtat cgttgtttca
    48301 ggaaacagcg aagagcagac caaagtggct gatgcattcg gcgaagcgct gagcgaagcc
    48361 ctagaccgac tacttccaga aggcgagaat atcatcgctg tgatgggagg aactactatg
    48421 gctgtggtag ccgaacaatt gtctaatctg gaaaataaga aacggcataa cctgtttgtc
    48481 cctgcaagag gcgggattgg tgaagcgata actgtacagg caaattctgt cagtgcaaga
    48541 atggctgcta aagcaaacgg taaccaccgt gcactttatg ttccggaaca attaagtctg
    48601 gcaacttaca attctttact caatgagcca tctatccaag aagtcttaaa cttgattagt
    48661 gaagcgaatt gtgttattca tagtattggg cgtgcattac acatggcagc acgtcgtaag
    48721 atgactgaaa aagaattagt catgctgaaa caagcaaatg ctgttgcgga atcttttggc
    48781 tactttttca atgaaaaagg tgaagtcgtt tataaggtcc ctagaatcgg catagaattg
    48841 caagatttgg aaaaagtccc gatcatcatg gcaatcgcgg gcggaaaatc aaaagctaaa
    48901 gcaattcgag cttatatgaa aaatgcacca aaacaaacgt ggctcatcac tgatgaagct
    48961 gctgcaaatg agattttaaa aggggtaacc ctttaaaata aatatttttt tgattttcat
    49021 aaggaggaaa tcttaaatga cagttaaagt aggtattaat ggttttggac gtattggacg
    49081 tctagcattc cgtcgtatcc aagatgtaga tggaatcgaa gtagtagcaa tcaacgactt
    49141 gacagatgct aaaatgttgg cacacttgtt aaaatacgac acaactcaag gacgtttcaa
    49201 cggaacagtt gaagttcatg aaggttcatt caacgtaaac ggaaaagaag ttaaagtatt
    49261 agctaaccgc aacccagaag aattaccatg gggcgaacta ggcgtagata tcgttctaga
    49321 atgtactggt ttcttcactt ctaaatcagc agctgaaaaa catttaacag ctggtgctaa
    49381 acgcgttgtt atctctgctc ctggcggaaa cgatgttcca acaatcgttt ataacacaaa
    49441 ccacgaaaca ttaactggta aagaaacagt tatctcaggt gcttcatgta ctactaactg
    49501 tttagctcct atggctaaaa cattgaacga caaatttggt gttgttgaag gattaatgac
    49561 aactatccac gcttacacag gtgaccaaat gactctagac ggacctcatc ctaaaggtga
    49621 cttccgccgt gcacgcgctg ctgctgcaaa catcgttcct aactcaacag gtgctgctaa
    49681 agctatcggt ttagtaattc ctgaattgaa cggtaaatta gacggagctg ctcaacgtgt
    49741 tcctgtacca acaggttcat taacagaatt agtaacagtt cttgaaaaag aagtaactgt
    49801 tgacgaaatc aatgcagcaa tgaaagaagc ttcaaacgaa tcttacggat acaacacaga
    49861 cgaaatcgtt tcttcagata tcgttggtat gacttacggt tcattatttg atgctacaca
    49921 aactaaagta atgacagttg gcgacaaaca attagttaaa actgttgctt ggtacgacaa
    49981 cgaaatgtca tacactgcac aattagtacg tactttagaa tacttcgcta acttataaga
    50041 tagacaaacg tacaagtgta aattatagac atgtacattt aaaagcgggg aagcaatcgc
    50101 gcttcttccg ctttttttga taataagttt attcaggagg aaataaaatg gctaagaaaa
    50161 cagtgaaaga tatcgattta aaagacaaaa aagttcttgt ccgtgttgac ttcaacgttc
    50221 ctttgaaaga cggcgtgatc actgacgaca ctcgtatcaa agcagcattg ccaacaatca
    50281 actacgttct agaacaaggc ggaaaagcaa tccttttctc tcaccttgga cgtgtaaaaa
    50341 cagaagaaga taaagaagga aaatctttag ctccagttgc aaaacgtcta ggcgaattat
    50401 taggtaaaga agtaacattc gtacctgaaa ctcgcggaga acaattagaa gaagcaatcc
    50461 gcaacatgaa tgacggcgac gttgtcgtat tcgaaaatac acgatttgaa gatattgacg
    50521 gtaaaaaaga aagcaaaaat gatacagaac taggtaaata ctgggcttca ttaggcgatg
    50581 tatttgttaa cgatgctttc ggtacagctc accgtgcaca tgcttctaac gtaggtatcg
    50641 cttcaactgg tatcccaaca gttgctggat tcttgatgga aaaagaaatc aagttcatcg
    50701 gtgaagcagt agaagaacca aaacgaccaa tgattgcaat ccttggtggc gcaaaagttt
    50761 ctgataaaat cggcgtaatc gaaaacttga ttcctaaagc agataaaatc ttgatcggtg
    50821 gcggaatgac ttacacattc tacgaagcaa aaggaatcaa gatcggtaac tctctagtag
    50881 aagctgataa agtggaacta gcaaaagaat taatcgaaaa agcaggcgac aaacttgtct
    50941 tgccaatcga caatgtctgt gcaccagaat tttcaaatga tgttgaaaca caagtaatcg
    51001 aaggagacat tcctgatggt ttgatggctt tagacatcgg cccagcatca gtaaaattat
    51061 ttgctgatac attacaaggt gcaaaaacag ttgtttggaa cggacctatg ggtgtatttg
    51121 aaatgagtaa ctttgctaaa ggtacaatcg gtgtttgtga agcaatcgct aacttagaag
    51181 gcgcaacaac aatcatcggt ggcggagact cagctgcagc agcagaacaa ttaggatttg
    51241 ctgacaaatt cacacacatc tcaacaggtg gtggcgcaag cttggaattg cttgaaggta
    51301 aagaattacc aggactagca gcaatcaacg ataaataaac gaccaagttt gctcaattct
    51361 gacgagtaat cgatcaaaag gtgaagggag ttttcatgac tccctagcca tttttcaata
    51421 aattaaggag tgtgttcctc aatgcgtaaa cctattatcg caggtaactg gaaaatgaac
    51481 aaaactgcat cagaagcaaa agaatttgct gaagcagtaa aaaacaaaat cccttcaaat
    51541 gacaaagttg attcagtcat cggatctcct gcattattct tacaagaatt aatgacatca
    51601 gctgaaggta cagaactaaa aatctcagca caaaactgct actgggaaaa ctcaggtgcg
    51661 ttcactggtg aaacttcacc agcagctctt gctgatctag gtgttcatta tgtgatcatc
    51721 ggacattcag aacgtcgtga atatttccat gaaacagacg aagacatcaa taaaaaagca
    51781 aaagctatct ttgcaaatgg tatgacacca atcctatgct gcggcgaatc tttagaaacc
    51841 tatgaagcag gcaaaactgc tgaatggatc gaaggacaaa ttacagctgg tttagctgat
    51901 ctttcagctg aacaagtgtc aaacatggtt attgcttatg aaccaatctg ggcaatcggt
    51961 actggtaaat cagctgacgc acaaatcgct gacgaaattt gtggagttgt acgtcaaaca
    52021 gttgaaaaac tttatggaaa agaagtatca gaagcagttc gtatccaata cggcggttct
    52081 gtaaaaccag aaaatattgc tgaatacatg gcaaaagaaa atgttgacgg tgctttagtc
    52141 ggtggcgcaa gcttgcaagc agattcattt ttgtcattgt tagacgctgt aaaataattg
    52201 ttgaaaattt gcgaaaaatg caagaaatgt ttgcactaat gagcaaaatt aactaaaatc
    52261 gtatatgagg gaaatccctt aggaaaaaac aaactcaaag gagagacaaa acatgtcaat
    52321 tattactgat gtttacgctc gcgagatctt agactcacgt ggtaacccaa caatcgaagt
    52381 agaagtatac acagaaagcg gagcttttgg ccgcggaatg gttccttcag gtgcttcaac
    52441 tggtgaatac gaagcagttg aattacgtga cggagacaaa gctcgttatg gcggtaaagg
    52501 tgtaactaaa gctgtcgaca acgtaaacaa catcattgct gaagcaatca ttggctacga
    52561 tgttcgtgac caaatggcta tcgacaaagc tatgatcgct ttagacggaa ctcctaacaa
    52621 aggtaaatta ggtgcgaacg ctattttagg tgtttctatc gctgttgcac gtgcagctgc
    52681 tgactaccta gaagtacctt tataccacta cctaggcgga ttcaacacaa aagtattgcc
    52741 aacaccaatg atgaacatca tcaacggcgg atcacatgct gataactcaa tcgacttcca
    52801 agaattcatg atcatgcctg ttggcgctcc aacattcaaa gaagctctac gtatgggtgc
    52861 tgaagtattc cacgcattag cttcaatctt gaaagctcgc ggattagcaa cttctgttgg
    52921 tgacgaaggt ggatttgctc caaaccttgg ttcaaacgaa gaaggttttg aagtaatcat
    52981 cgaagcaatc gaaaaagctg gctatgtacc tggtaaagac gttgttcttg ctatggatgc
    53041 tgcttcttca gaattctacg acaaagaaaa aggtgtttac gtactagctg actcaggcga
    53101 aggcgaaaaa acaactgacg aaatgatcaa attctacgaa gaattagttt ctaaatatcc
    53161 aatcatctca atcgaagatg gattagacga aaacgactgg gacggattca agaaattaac
    53221 tgacgtatta ggcgacaaag ttcaattagt tggtgacgac ttgttcgtaa caaacactca
    53281 aaaattatct gaaggtatcg aaaaaggaat tgctaactca atccttatca aagtgaacca
    53341 aatcggtaca ttaacagaaa ctttcgaagc tatcgaaatg gctaaagaag ctggctacac
    53401 agcagttgta tctcaccgtt ctggtgaaac agaagattca acaatctctg atatcgctgt
    53461 tgcaacaaat gctggccaaa tcaaaactgg ttctctatca cgtactgacc gtattgctaa
    53521 atacaaccaa ttactacgta tcgaagacca acttggtgaa gttgcagaat acaaaggttt
    53581 gaaatctttc tacaacttaa aaaacaaata attcttttct aatgaagata catgatcgac
    53641 tattgaaaga tagtcgagta tgatcactta gttaagtaat cgaactctct acttgctgaa
    53701 atatcagcag gtagagagt
//
LOCUS       DAZKLA010000004        49425 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000004 DAZKLA010000000
VERSION     DAZKLA010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.004"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(255..1982)
                     /locus_tag="ACG85N_000163"
     CDS             complement(255..1982)
                     /locus_tag="ACG85N_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFQ0222970.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1975..3168)
                     /locus_tag="ACG85N_000164"
     CDS             complement(1975..3168)
                     /locus_tag="ACG85N_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0222971.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3355..3999)
                     /locus_tag="ACG85N_000165"
     CDS             complement(3355..3999)
                     /locus_tag="ACG85N_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0222972.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4093..4227)
                     /locus_tag="ACG85N_000166"
     CDS             complement(4093..4227)
                     /locus_tag="ACG85N_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFQ0222973.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4229..6361)
                     /gene="feoB"
                     /locus_tag="ACG85N_000167"
     CDS             complement(4229..6361)
                     /gene="feoB"
                     /locus_tag="ACG85N_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFQ0222974.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6358..6831)
                     /locus_tag="ACG85N_000168"
     CDS             complement(6358..6831)
                     /locus_tag="ACG85N_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFQ0222975.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7232..7456
                     /gene="nrdH"
                     /locus_tag="ACG85N_000169"
     CDS             7232..7456
                     /gene="nrdH"
                     /locus_tag="ACG85N_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFQ0222976.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7615..9774
                     /gene="nrdE"
                     /locus_tag="ACG85N_000170"
     CDS             7615..9774
                     /gene="nrdE"
                     /locus_tag="ACG85N_000170"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFQ0222977.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9824..10789
                     /gene="nrdF"
                     /locus_tag="ACG85N_000171"
     CDS             9824..10789
                     /gene="nrdF"
                     /locus_tag="ACG85N_000171"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFQ0222978.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10878..12017)
                     /locus_tag="ACG85N_000172"
     CDS             complement(10878..12017)
                     /locus_tag="ACG85N_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0222979.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12326..13039)
                     /locus_tag="ACG85N_000173"
     CDS             complement(12326..13039)
                     /locus_tag="ACG85N_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFQ0222980.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13293..13994)
                     /gene="nagB"
                     /locus_tag="ACG85N_000174"
     CDS             complement(13293..13994)
                     /gene="nagB"
                     /locus_tag="ACG85N_000174"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFQ0222981.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14228..15760
                     /locus_tag="ACG85N_000175"
     CDS             14228..15760
                     /locus_tag="ACG85N_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0222982.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15951..17153)
                     /locus_tag="ACG85N_000176"
     CDS             complement(15951..17153)
                     /locus_tag="ACG85N_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0222983.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17235..17924)
                     /locus_tag="ACG85N_000177"
     CDS             complement(17235..17924)
                     /locus_tag="ACG85N_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0222984.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18030..18398)
                     /gene="rplL"
                     /locus_tag="ACG85N_000178"
     CDS             complement(18030..18398)
                     /gene="rplL"
                     /locus_tag="ACG85N_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFQ0222985.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18451..18954)
                     /gene="rplJ"
                     /locus_tag="ACG85N_000179"
     CDS             complement(18451..18954)
                     /gene="rplJ"
                     /locus_tag="ACG85N_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFQ0222986.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19158..19285)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19364..20053)
                     /gene="rplA"
                     /locus_tag="ACG85N_000180"
     CDS             complement(19364..20053)
                     /gene="rplA"
                     /locus_tag="ACG85N_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFQ0222987.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20157..20579)
                     /gene="rplK"
                     /locus_tag="ACG85N_000181"
     CDS             complement(20157..20579)
                     /gene="rplK"
                     /locus_tag="ACG85N_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFQ0222988.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            20922..21590
                     /gene="sdaAB"
                     /locus_tag="ACG85N_000182"
     CDS             20922..21590
                     /gene="sdaAB"
                     /locus_tag="ACG85N_000182"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFQ0222989.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21603..22475
                     /gene="sdaAA"
                     /locus_tag="ACG85N_000183"
     CDS             21603..22475
                     /gene="sdaAA"
                     /locus_tag="ACG85N_000183"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFQ0222990.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22566..23666)
                     /locus_tag="ACG85N_000184"
                     /pseudo
     CDS             complement(22566..23666)
                     /locus_tag="ACG85N_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(23758..24645)
                     /locus_tag="ACG85N_000185"
     CDS             complement(23758..24645)
                     /locus_tag="ACG85N_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0222991.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(24722..25267)
                     /gene="nusG"
                     /locus_tag="ACG85N_000186"
     CDS             complement(24722..25267)
                     /gene="nusG"
                     /locus_tag="ACG85N_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFQ0222992.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25368..25538)
                     /gene="secE"
                     /locus_tag="ACG85N_000187"
     CDS             complement(25368..25538)
                     /gene="secE"
                     /locus_tag="ACG85N_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFQ0222993.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25562..25714)
                     /gene="rpmG"
                     /locus_tag="ACG85N_000188"
     CDS             complement(25562..25714)
                     /gene="rpmG"
                     /locus_tag="ACG85N_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0222994.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            25876..26520
                     /gene="cbpA"
                     /locus_tag="ACG85N_000189"
     CDS             25876..26520
                     /gene="cbpA"
                     /locus_tag="ACG85N_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFQ0222995.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26576..27079)
                     /locus_tag="ACG85N_000190"
     CDS             complement(26576..27079)
                     /locus_tag="ACG85N_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0222996.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27092..27138)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            27676..28641
                     /locus_tag="ACG85N_000191"
     CDS             27676..28641
                     /locus_tag="ACG85N_000191"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0222997.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(28740..29912)
                     /locus_tag="ACG85N_000192"
     CDS             complement(28740..29912)
                     /locus_tag="ACG85N_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0222998.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(29899..31044)
                     /locus_tag="ACG85N_000193"
     CDS             complement(29899..31044)
                     /locus_tag="ACG85N_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0222999.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31058..31982)
                     /locus_tag="ACG85N_000194"
                     /pseudo
     CDS             complement(31058..31982)
                     /locus_tag="ACG85N_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32099..33178
                     /locus_tag="ACG85N_000195"
     CDS             32099..33178
                     /locus_tag="ACG85N_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0223000.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33175..33803
                     /locus_tag="ACG85N_000196"
                     /pseudo
     CDS             33175..33803
                     /locus_tag="ACG85N_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(33913..34140)
                     /locus_tag="ACG85N_000197"
     CDS             complement(33913..34140)
                     /locus_tag="ACG85N_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0223001.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34243..36831)
                     /locus_tag="ACG85N_000198"
     CDS             complement(34243..36831)
                     /locus_tag="ACG85N_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFQ0223002.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(36851..37510)
                     /locus_tag="ACG85N_000199"
     CDS             complement(36851..37510)
                     /locus_tag="ACG85N_000199"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0223003.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(37559..38062)
                     /gene="trmL"
                     /locus_tag="ACG85N_000200"
     CDS             complement(37559..38062)
                     /gene="trmL"
                     /locus_tag="ACG85N_000200"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFQ0223004.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38283..39149)
                     /locus_tag="ACG85N_000201"
     CDS             complement(38283..39149)
                     /locus_tag="ACG85N_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0223005.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39258..39899
                     /locus_tag="ACG85N_000202"
     CDS             39258..39899
                     /locus_tag="ACG85N_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFQ0223006.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(39961..41322)
                     /gene="mgtE"
                     /locus_tag="ACG85N_000203"
     CDS             complement(39961..41322)
                     /gene="mgtE"
                     /locus_tag="ACG85N_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFQ0223007.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41340..42239)
                     /locus_tag="ACG85N_000204"
     CDS             complement(41340..42239)
                     /locus_tag="ACG85N_000204"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0223008.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42241..43038)
                     /locus_tag="ACG85N_000205"
     CDS             complement(42241..43038)
                     /locus_tag="ACG85N_000205"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFQ0223009.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43016..43720)
                     /locus_tag="ACG85N_000206"
     CDS             complement(43016..43720)
                     /locus_tag="ACG85N_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFQ0223010.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            43851..44427
                     /locus_tag="ACG85N_000207"
                     /pseudo
     CDS             43851..44427
                     /locus_tag="ACG85N_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            44545..45222
                     /locus_tag="ACG85N_000208"
     CDS             44545..45222
                     /locus_tag="ACG85N_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFQ0223011.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45301..47109)
                     /gene="pepF"
                     /locus_tag="ACG85N_000209"
     CDS             complement(45301..47109)
                     /gene="pepF"
                     /locus_tag="ACG85N_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFQ0223012.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47285..48283)
                     /locus_tag="ACG85N_000210"
     CDS             complement(47285..48283)
                     /locus_tag="ACG85N_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFQ0223013.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(48509..49162)
                     /locus_tag="ACG85N_000211"
     CDS             complement(48509..49162)
                     /locus_tag="ACG85N_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFQ0223014.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        14768 a        10391 c         8716 g        15550 t
ORIGIN      
        1 tattctttaa tattttttga gaatgcactt cgtacataat aaactttaat cacattagtc
       61 tttcatacct acgatcgatc tctttgactc gcacccataa attcataata gtttgtcggc
      121 gtctttggac gtttcgaaga tgagctacgc attctctttt tttatagaca aaaaagatag
      181 agacgaacat ggagcttcca aatttggata tttgatccaa ttttggaagc tccatttatt
      241 tcgtgtctgt ctttttattt tttccagctt tcgactaatt cttggttctc tttgatccag
      301 tcttttgctg cttgctgtgg atcttttcca ttgttgattt ccaacataac tttttccata
      361 tctttttctg accaatggaa attgtctaat actttgtaag cttctggctg gtcttctttc
      421 aatccttttc gaaccatcgt atggatctgc tcttcgcttc ccattccgtt ttcaggatct
      481 tctaagtatt tcagatcata tttggcaaac atccaatgag gtgtccatcc tgtaactaca
      541 attggttcgt gttttttgat cgcttgacct agtgcaacgg tcattgctcc tgaagaagat
      601 gtctctactt tccagtcttt caagttgtca tatttttgga tcgtgttttc agcagccgta
      661 actacaccag ctcctggctc gattcctatg attttcttac ctgcctgatc ggttaactca
      721 tcgatcgaat ttacatccat gtaggctggc actgctaaac caacttttgc tcctgtcaaa
      781 ttggcaccta ggtcttcgac ttgatcttta tattgtgcat attgtgaacc atgagtttta
      841 ggtagccaag ctgaaaccat ggcatctgac tcgccatttg aaacagattt ccacatgatt
      901 gaattatcta atggcgtcat cgttacatca tagcccattt gtttcaatac ttcaccgact
      961 acatttgttg aagcaacttc tgtatcccat tcaacataag ataagttgat acgttttgtt
     1021 tctttggcag atgaaaaact ccctatacca atcgcaccta agatcactac tactacagca
     1081 ccaatgatca gtccttgacg ttttttactt ttctgtgttg ttgctcctgc agctttttta
     1141 ttgtttggct ggttcaaatg ctgtgtaaaa cggtcaatga tgattgctaa gataactaat
     1201 gcaaccccgt tgacaaatcc attcccgact tgtgctcttt gcaatgctga taatacgccg
     1261 cgtcctaagc cgggtgcacc gatcatagag gcaataacga ccattgaaag tgacagcatt
     1321 gttgtttgat tgatccctgc catgatcgta cttttagcta aaggcaattc tagtttgaat
     1381 agtttttgac gacttgtgct cccgaatgaa tcagaagctt ctactaattc tttaggaact
     1441 tgtcgaatcc ctaaattcgt aaaacgaacg gttggtggca gagcaaagat gactgaggca
     1501 aatacccctg gaaccatccc gataccgaaa aatgcaactg ctggaatcaa ataaacgaat
     1561 cctggcattg tctgcataaa gtcaaggatc ggtttgataa tactttgggc tttactgctt
     1621 tttgccatca agatccctaa tggtacccca atgatgatcg agatcacact agataaaaga
     1681 acaagtgtga cagtattcat caaatcattc catagacctt ggttataaat aaaccataga
     1741 ccgattaatg tgaacaacgt aagtcctgga cgtttcttag aaataaagaa agctgctacc
     1801 gtcaataaca agataaacaa aggagctgga atcactaaca gtaaatttgt aatccccgac
     1861 atcactgcct gtccaatcgt ttgtaaaaat gaaaataggc cagaaaaagt tgtcgtaaac
     1921 cattctgtga tattttctac ccagtttgcc actggtaatt gatagttcaa tagattattc
     1981 atgttcactc acctcgcttg tttctgctaa tgcttcaatc acactacctc ggatgacgac
     2041 accttttaat tttccatcct ctacaactgc aagtggagtc ggtgagtcat aaataatatt
     2101 gaaaatatca cttaccacca tgtctttccc aatcaccgtc acattttcat ctaggaagtc
     2161 gatcaatgga cgtttttgct tcctagcttc taacgcttga tctgcagtga tgatcccttt
     2221 caggtgtctc tttctgtcaa cagccatcag catgctgact tcttcattac gcatccttgt
     2281 caatgcaacg tttggtccat cagattcgat attcgtcgtc aatgcaggta ccatgatgtt
     2341 ttgagccgta agcactttgg aacgatcgac ttcttccaca aattcacgta cataatcatt
     2401 tgctggattg gtcaatattt cttcacctgt tccgatctgc atgatttcgc cgtctttcat
     2461 caaggcgatt ctgtccccaa tccgtaaagc ttcattcaaa tcatgggtaa tgaaaataat
     2521 cgttttttga acattggctt gcaggtccag taattcgtct tgcatttctc gccggatcag
     2581 cggatcaaga gccgagaacg cttcgtccat cagcaagatc tcaggatcat ttgctaaagc
     2641 tctggctagg ccaacccgtt gttgcatccc accagataac tgactaggat attggtcttt
     2701 aaatgaaagc aaactagaat tttctaatgc tttttctgct ttttcttgtc gttcttcttt
     2761 aggtacacca cggacttcta acccgtattc tgtattttcc aagatcgttc gatgtggaaa
     2821 cagaccgaag ttttggaaaa ccatattgat tttatgacga cggacttctc gcaattcttc
     2881 tttgcttaac ttcgatacat cttctccatc aatatagata ctgcccgaag ttggctcaat
     2941 cagacgattc aatagacgga tcaatgtgga tttcccactc ccggaaagcc ccatgatcac
     3001 gaagatttct ccttctttta cgtcaaaatt cacgtcatac acaccaaccg ttgcaccggt
     3061 tttctttaaa atctccgttt tcggctggtg atcattcatc atatctaaag cggcttgtgt
     3121 ctttttacca aatatctttg ttaaatgttt tacttctact tttaccaaac aaattctcct
     3181 tttttatgta aattcatttt tctttctgaa aaaagtgacc acactatgtt aacatcatgt
     3241 agtcactttg tcaaaaatga aagaaatttg cttgtccaaa ctgaaaaaaa cttggctaaa
     3301 tctttgtaaa taaaaggttt agccaagtta atattttttt aatatttcac acaatcattc
     3361 tgcaagctga tgctccgggt aaaaaaagtt tgtcatacgc tgtagtgtat attcatttcc
     3421 aataaaatga acgatgtccc ctgccgtaaa tttagcatag gggccaggtg aaacaagcag
     3481 ttcttcatta tgagaaattg cgataactgt ggcgccagtc gcctgccaga tattcaactc
     3541 gctaattgaa cggtccagat gcattgcttt ttctgataaa gataattcaa acggaagtaa
     3601 aggattcatc ttatgtattc gcttcgtttg attgaccagc tgatccaata attgagaaaa
     3661 atgattcagc tcatcttgtt gcttctccac acttgccatg atttctgctt ttgtatcttg
     3721 gatggattgt acatcttgat attgcaatag gaaatctttt gctttttcct tcgaagcaac
     3781 aaatgcccca cttccatgcc gtacttccat gattccaaga tctaccagca cattgatagc
     3841 cttgcgggta gtctcaggag aagcattgta cgtactggca agtgttgagc gggcatggat
     3901 cttctcaccc actttatatc ggttttcaac gatacgttcg gctaaatcaa cagcaatctg
     3961 ctggtagcgc gggcggacga tttttttctt tccagacatt ttctttcctc tttctttttt
     4021 tactttccct catcttacta tgctctagca aaataagcaa aaaaggggag aatagcctct
     4081 tgcaacgact gatcatcgct tgatctgttc cttttttact gggcaagctg tttgacagtc
     4141 atcacaattc ttaccggatt ttaaacgagt atagacaatt gcgcctgcac ttccaaagat
     4201 taacacactt aataagaatg tcgccatcct attcctctcc ttctaatgac aatatagtat
     4261 aaaactggtt cttggatttt ggctgacgga acaaaacata aacgagtcct atcatcagta
     4321 aaatagcaag atacgtccct aacgctacct gtccaccctc caataagtgt cccaactgat
     4381 aaatcacaaa actaactaaa taagctaaac cgcattgata gcctatggcg atccaagtcc
     4441 atttcttggc attcatttcc cgatggatag ccccaattgc cgcaaaacaa ggcgcacaaa
     4501 gtaaattgaa ggctaacaat gaataagctg ctgcgggagt atatgtgtgt tgtagcactt
     4561 gccagatttc tctgccattt tcggatactt ccgcatgacc aaacaaaata ccaaatgtgc
     4621 caataatatt ttctttagcg accaatcctg taatcgctgc gactgttcct tgccagtttc
     4681 cccaaccaag cggctgaaaa acaactgcca ggattcgccc gaaaaaggct aagatacttt
     4741 ggtcttcaga aacaggttga gcataaaaat tataagaaga gctgaaccag atcacgatac
     4801 tagaaacaaa gataatcgtt cctgcttttt taacaaaaga cttgctgcgg tcataggttt
     4861 gtcttaggac cgctgaccat tgaggaaaat gatatgcggg caattccatg ataaaaggag
     4921 ctgtatctcc agcaaacaaa cgtgtttttt tcaagctgat tccagagaaa ataattgcag
     4981 tcataccaag aaaataggca gaaggagcta cccaagattg atgaggaaaa aaggcccctg
     5041 ctactaaggc aataatcggc aactttgcag aacaaggcat gaaggttgtg accatgatcg
     5101 tcaaacgacg atcttgctcg ttttcaatcg tgcgactggc catgacgcct ggcactccac
     5161 atcctgtagc aatcagcata ggaatgaagg attttcctga aagaccgaat ttcctgaaaa
     5221 gtcgatccat gacaaaagcg atcctcgcca tatatccgca gtcttccaga aagctcaagc
     5281 ataaaaacaa tataatcaat tgcggtagaa atcctaacac tgctcccacg cctgcgatga
     5341 tgccgttcaa gatcagctcc tgcatccatc ccgctacttg ccattcagct aacaatctgc
     5401 caacatgttc tggtaaccat gtaccaaaca atgtgtcatt gatccaatct gtccccattg
     5461 ttcctactgt ttgaatagcc agataataga tcagccacat cacaaatgcg aagatcggca
     5521 aggctaacca acgattcgtt gcaatctgat cgattttgtc actcatcgat aattggaaag
     5581 aatcatttga cacaacacag agtgtacata gttgggtaat aaattcataa cgttcattga
     5641 ccagaatcgt ctcactatcg tcgtccaata gtttctctgt taactggatc agttgctcga
     5701 tctctttttg ctgagaaact gctatctcgt aatcctctct taccttctga tctcgttcaa
     5761 aatatttcat tgcataccag cgtttcttgc cgctttgttc tcctagtaca tcttcaattt
     5821 catgtaaagc tgcttctaaa cgtggatcat atgtacaggg aacaggaata gaaggccgct
     5881 tttttgcaga ctcctgaatc ttttcgatta gtttttcgat tccttgcttt ttcaatacgc
     5941 tgattggtaa aactggtaca ccaagaccat aggaaagctt ttctagattg attttttttc
     6001 cttctttttt gatgacatcc atcatattca gaccaatgat cataggaatg tctaaatcca
     6061 ataactgcgt agttaaataa agatttcgtt ctaaattggt agcatcaata atgttgatga
     6121 tcagttcagg ctgttcctga accaaataat ctcttgcaat cacttcttct ggtgtataag
     6181 gagacaaaga atagatgccg ggtaggtcct gaatatgaat gttcggattt tttttcaacg
     6241 atccttcttt tttttctact gttacgcccg gccaattccc tacatattga ttcgaaccag
     6301 tcaagagatt gaacaagcta gttttcccgc tgtttggatt tccgactaac gcgatattca
     6361 ttctttttcc tccctttgta tcaagattag ttcggcctct gactttcgta acgtcagttc
     6421 ataccctcgc actgtcagtt cgattggatc tcccagaggg gctagttttc ggataactat
     6481 ttttacattt cgcgtcaatc ccatatccat taaccgtcgc ttcactgcgc cttgtccgtg
     6541 gattgctacg acgatcccag tctctcctgc ttttaattga tcaagagaag tccagctttc
     6601 agaggaagca gtctttttat tgacaaagat ttgacctagt atttcccgac ttactgccac
     6661 tcgatgactt tttaccagta aaatggcatt gttgcctgat aaattgacga cgacgatttt
     6721 acttccggca acgatcccga tattttgcaa atggcgtttt gttttttctt ctgtttcgat
     6781 tcgttcgact tgataaacga aattctgctt tgcctgcgct aaggtctgca tattgattcc
     6841 tctcttgtga aataaatcat gtattaaaat cattcattat atgttctaat tttcttacgt
     6901 gttacgcaaa taaatgagaa tcattctcac atagaatact ctcggtaatt gagaatgtca
     6961 attatacttg tgatattatg agcgaatctt tagtattgat agataaaaat gagaaaaaaa
     7021 tatttcttca tagttttcca caattttttc tctattaaat ttgctatttt cccacacgat
     7081 acttgttttc agctcttttt tcaaaaacac aaaacttagt attccacagt ttccacaaca
     7141 caatatgtag tgtttgttgt ttgacttttt tactaagagt tttatactat ctattataga
     7201 taataagaat gtgttagagg aggcactcac catgaacgta aaaatctttt ctaaaaataa
     7261 ttgtatgcaa tgtaagatgg tcaaacggtt tttaacagaa aatcaaattg cttttgaaga
     7321 agtcaatatc gatgaacagc ctgaagcagt agactggttg aaagcacaag gattccaaag
     7381 tgttcctgtc attacttctg atgcagaaac agttatcgga ttccgtcctg atcagttgcg
     7441 taaacttgcc agctaacatg actaaaagaa tcaaaacgtg atacatggat ttgaagggct
     7501 tgagacagca ccagtttttg tcgcaaactt ccaaatcctt ttttaaagct taaaaacaat
     7561 gcaatgattt gcaatcaagc atcatcaaat acgaaatgaa ggttgggcgt acgtatgagt
     7621 cttaaaaatt tacaagatgt cagttatttc aagctgaata atgaaatcaa tcggccagtc
     7681 aatggtcaaa tccctttgaa taaggataaa gaagctttag ttgccttttt tgaggaaaat
     7741 gtcaaaccaa atacgatgac ttttctatct attatggaaa aaatcaatta cttgatcgat
     7801 caagaatata tagaagcaga attcattcaa ttgtattcac catcatttat tgaaaagcta
     7861 tatcaatttt tggacgagca acatttttct ttcaaatctt ttatggctgc ttacaaattt
     7921 tattctcaat atgcgttgaa aaccaacgac ggttccgctt atctggaaac ttatgaagat
     7981 cgagtagcct tcaatgcgct ttactttgcg aacggggatg aagaattagc acttgctctt
     8041 gcagacgaaa tgatccatca acgatatcag ccagctacac cttctttctt gaatgctgga
     8101 cgtaaacgtc ggggcgaatt ggtgtcttgt ttcttgactc aagtcactga cgatatgaac
     8161 tctatcgggc gctcaatcaa ctctgctttg caattgtccc gtattggtgg cggcgtaggg
     8221 attactttat caaatcttcg tgaagccggc gctcctatca aaggatatga gggtgctgca
     8281 agtggtgtcg ttccagtcat gaaattattc gaagatagtt tcagctattc taatcaatta
     8341 ggtcagcgtc aaggagctgg cgtggtttat ttgaacgttt tccatccaga cattattgct
     8401 ttcctttcag caaaaaaaga aaatgcagat gaaaagattc gcgtaaaaac tctttctctt
     8461 ggcgtgatcg tacctgataa attttatgaa ttgacacgta aaaacgaaga tatgtattta
     8521 tttagtccat atagtgtcga aaaagaatat ggtgtcccat tctcttatgt aaatattaca
     8581 gctgagtatg atcatctagt tgcaaatcca aacattcgca aacaaaaaat caaagcgcgt
     8641 gatcttgaaa atgaaatctc aaaattacaa caagaatctg gctatcctta cgtaatcaat
     8701 attgatacag cgaaccgcga gaatccaatc gatggcaaga tcatcatgag taatttatgt
     8761 tcagagattt tacaagttca aacgccttct gtgattaacg gcaaacagga atatgaagta
     8821 ttaggaacgg atatctcatg taacttaggc tcgacaaata tcgtgaattt gatgcaaagc
     8881 cctaactttg gtcaatctgt gcgtacgatg actcgtgcat tgacatttat tacagatgca
     8941 tctgatatcg atgtcgtacc accgatccaa aacggaaata agctgaatca tacgattgga
     9001 ttaggtgcca tgggcttaca cacttatctt gcaaaagaac aaatggaata tggctcagag
     9061 gattcattag actttacaaa tatttacttc atgctgttga attattggac acttgttgaa
     9121 agcaacaata tcgctcgtga aagaaaacaa gttttccata attttgaaaa atcagcttat
     9181 gctgacggca cttatttcga aaaatacgta actggagaat tccagccaaa atcagaaaaa
     9241 gtcaaagcat tgtttgaagg catttttatc ccaactgctg aagattggaa tgctttgaaa
     9301 caagcagtaa tgaaagatgg actgtatcat caaaatcgct tggcagtagc accaaatggc
     9361 tcaatttcat atatcaacga tacgagtgca agtattcatc ctatcactcg catgatcgaa
     9421 gaacgccaag agaagaaaat cggtaaaatt tattacccag cgccttatct atccaatgag
     9481 acgattccgt attacacttc tgcttatgat atggacatgc gtaaagtaat cgatgtttat
     9541 gcaatggcac aacaacacgt cgatcaaggc atgagtttga ctttattcat gcgttctgaa
     9601 atcccagaag gcatttatga atggaagaca acaacgaagc aaacgacacg tgatttgaat
     9661 attttaagac attacgcttt ccacaaagga atcaaatcca tttattacgt acgtaccttt
     9721 actgatgatg cagaagaaat cggaagcaat caatgtgaaa gctgcgtgat ctaatatcat
     9781 agatagattc gcttattcat attttaatta aaggagcatt ctcatgtcag aaacatatta
     9841 tgaagcaata aactggaatg aaattgaaga tatcattgat aaatccactt gggagaagct
     9901 gacagagcaa ttttggctag atacacgtat cccactttca aatgacctag atgattggcg
     9961 tacattgtct caattggaaa aagatacagt aggacatgta tttggcggtt tgacactgct
    10021 agatactgtc caatctgaaa gtggcatgga tcagttgcgt aaagatgtcc gcactcccca
    10081 tgaagaagcc gttttgaaca atattcaatt catggaatcc gttcatgcca aaagttattc
    10141 ttctattttt agtacactga atacgaaaaa agaaatcgaa gaaatttttg actggacaaa
    10201 tacgaatcct tatctgcaaa aaaaagctga gcgtatcaat gaaatctaca aacacggtac
    10261 accgctagaa aagaaaattg ccagcgtctt tttagaaaca ttcttgttct attcaggttt
    10321 ttatactcct ctttattatt taggaaacaa caaattggct aacgtagcag agatcatcaa
    10381 actaatcatc cgagatgaat ctgtacatgg aacctacatc ggttacaaat tccaattagg
    10441 attcaacgag ttacctgaag cagaacaaga aacgttaaaa gactggatgt acaatcttct
    10501 ttatgagctt tacgaaaacg aagaacgtta tacagaagaa ttatacgatc caatcggctg
    10561 gacagaagaa gtcaagacat tcctacgcta taatgccaac aaagcattga tgaacctagg
    10621 gatggatcca ctctttccag atactgccaa tgatgtcaac ccaatcgtaa tgaacgggat
    10681 ttcaacaggc acaagcaacc atgacttctt ctcacaagtc ggaaacggct atttgttagg
    10741 tcatgtagaa gcgatgaaag acgacgatta tttgattgga ttggaataag gatttatttt
    10801 aattcaaaaa tttttcgaaa aataaaaaga gtttagcgta cagatatttt aaatgtacac
    10861 tgaactcttt tttatgctta ttcacttgtt tcttccagca cttttggact gtctaacttt
    10921 tccgaaacat gttccattct tctcaataaa tagattttcc ggatatttaa tattatcgtc
    10981 ttaatcactg catagacagg tatgcaaatc agcatgccta atattcctgc gacacttcct
    11041 gctccaatca ataccaagat gatcgtcaaa ggatggatat tcattgattt cccaaataac
    11101 aacggcttga tcaaatttcc atctaattgt tggatcacta aaatcgacaa cgccatatac
    11161 aaagcttgaa atggagagat aaacaaaccg acaatgattg ctggtaccgc tccaataaaa
    11221 gggccgacat acgggataat attcgtgatt ccgcaaatga ccgcaagcaa caagctataa
    11281 ggctgatcga atattctcat tgccaaaaag ctcattacac caataatcaa agcatctaag
    11341 atcgtactgc tgatataggc agataatgtt tcgtttaatt cttttaccgt ttggcgtaac
    11401 tctttacgaa tcgctttcgg aaagaagtga gacaacgcat caataaattt gtgcccatcc
    11461 ttgaacataa atagcaaaat aaatggaacg gtaaataaca aaataaaaaa ttgcatcata
    11521 aatgaaatga tttttgagac acttgcagat aaactgacta atatgatatt gaacaaatta
    11581 ctaagagata aatttgctga tgctaattgt tcttccaaat tgaaattttt aaactcttcc
    11641 cgctgcgcca attcatttaa ccatttgctt gtttcatctg catacattgg caagctctgg
    11701 gtcaattgaa ttgtctgttc cattaactga ggcactacat tcatgacagt taaaacaact
    11761 aaaatgatta cgaccaaaaa cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt
    11821 tcgagaaaaa caacgattgg atcgaacata taatagataa aaccagcaac aagcaatggt
    11881 aaaaaaactg ccgaaagcat tttcaatatt ggactaaaaa tattcggcat ttgtaaaata
    11941 aagtacacgc cgatgatcgt gagtactagc tccactgtcc agaaaaataa ttttgattgt
    12001 ttaaaacgtg attgcataca tctcctccaa ggtcctttga tacttctatt ctatcattta
    12061 acaaagagtt acgcgctgag attttcattt gcttaaaaaa aacaaaaaaa ataaacatat
    12121 tttaatgagt agacgagtac ttaaacaaaa acacggtttg gagtagtgac aaaaattata
    12181 ctgcagtccc tttttccaaa taaacggtgt tcaaaagctt gcatcttcgt aataagccca
    12241 gccaaacgaa aaatataagg aactattttt atttggctgg gcttattact tgatgtagga
    12301 catctatttt acaacctcag atcatttatt gcgcaatact gactaaatgc aactttgtga
    12361 tatttttgtg tacggacaac tcctcgatca catcggcata agtgagccct tttggaagat
    12421 caatcgtata gatatttttg tacactcggt agtcatcaac gaattttacg tcaaaattca
    12481 catcttcgat tacgatattt ttctcttcaa aataatcggt aagaaaatct ttggtttctt
    12541 ttctatggat aaattggatt tctaactttt tagtcgtagg tacatggatc acgcgtttca
    12601 ctactgtgag tgcaatagca atcccgataa aactgatgat cgcaatggaa tagtaaccca
    12661 tgccgatggc gatccctaat ccagcaattg cccataaaga agcggcagtc gtaagtcccg
    12721 tcaccgattg tttcgtgacg atgatcgttc ccgcgcctaa gaaaccgaca ccactcacca
    12781 cttgagcaat cagccttgct tcatctgaac gaatcgttcc agcaagctgt ggatactcta
    12841 aagcatctct taatgcacca ctagcgatct caacttggat caatgcaatg atacatgctc
    12901 ccatacaaac taaaatatgc gtacgcatac ctgctggacg atttttatat tgtcgttcaa
    12961 aaccgattgc tccgccaaat agcatagcga tccctaaacg taaaataatc tctggaattg
    13021 tcaataccat atcattcatc taaacaccct ccagtccaat atatcatgtc tgaaaggagc
    13081 agacaaaaaa agtgcaattt ttacctctta cgaaaaaagg actgtgacaa aatttttgtc
    13141 atagtccttt ttatgaataa acggtgttca atcagctgac cctcggtatt actcaacaaa
    13201 cggcaaaata tggggagcta tttttccttt gtcttctcta atactcaggt caaaacgctg
    13261 atttcacaac ctctttagcc tactattgaa aattacaact tacttgctgc ttcttcatcg
    13321 atgatcacaa caacattgtc gtgattttgc aatacacttg ctggcaaatg attcgttaca
    13381 ggtccttcaa tcatcccttt gattgcatca gctttgcttt cgccaaatgc catcaagacg
    13441 atttctttac ttttcataat tgaaccgatt cccattgaaa cagcttttgt tggtacatct
    13501 tctgctttat cgaagaatcg tttgtttgca ttgattgttg attctgttaa gtcaactact
    13561 gaagtcgttc cgtcaaatga agcaccaggt tcattgaaac cgatatgtcc attttgcccg
    13621 attcctaaga tttggatatc gattggatgt gcatcgatga tttgatcata ctttttgcat
    13681 gctgcttcca gatcttctgc ttttccatct ggtacaaacg tttctttgaa tggtttttta
    13741 tcaaataatt ggacatccat gaaatgacgg tagctttgtt catctgaacc gcctagtcca
    13801 acgtactcat ctaagtttac agaagtcatt tcactgaaat ccaaatcgct tgaagtcatt
    13861 tctttatata aagtaactgg tgtgcttcct gttgccaatc ctaatacttt tgcacctttg
    13921 ttcatacctt ctttgatgat ttcaaatgct ttttttccgc cttcttcagc gttttttaca
    13981 cgaatgattt ccatagtgat tgctccttta tattttggta tagcccaatt atagcattct
    14041 ttttttcttt ttacaataat tagtatagac caaattgcat ttttttcatt ttcttgacaa
    14101 taaataggaa tccgtgttag gtttcataag atggttcaat caaacaatcc atgttgcatg
    14161 aaaaccaagc aagtgattca ctgtggacgc tttatgtctg cagttttttt atgaaaaagg
    14221 agttattatg agtatactga cattaaacaa cattacccag caatttggcg acaaaatcct
    14281 ctatgaaggt gtcgatctgc aattaaatgc tggcgaacat cttgggctga ttggacaaaa
    14341 cggagccggt aaaagtacat tgattaaaat catcactggt gagatcttac cagatgacgg
    14401 acagatccat tggcaaaaaa atattcacaa aggttacctc gatcaatatg tggaagtcga
    14461 tgaaacatta acaatcgaag aatttctaaa aactgcgtac gcaaaagaat acgaaaagga
    14521 agaaaaaatt gcttttcttt accaaacata tagcgagacc ttcgatgatt ccttgctgga
    14581 aaaagctggg aaactccaaa cggaattaga ccaaggagtg ttttatcaaa tcgacacctt
    14641 agttgctgag atgagcagcg ggttggggat cgatgtctta ggtttagatt cactgttgaa
    14701 aaatctaagt ggtggacaac gttcaaaggt gattttagca aaactacttt tagaaaaccc
    14761 agatgttctg atcttagatg aaccaaccaa tcacttagat gatcagcata tccaatggct
    14821 gacgcagttt cttcaggatt ttgacggtgc gtatatcgtg atttcccatg accaagaatt
    14881 tcttgatcag atcaccaccc atatcgcaga catcgagttt ggaaagatta ctaagtatac
    14941 tggccatcta aaacaagcat tgaaacaaaa ggaacaaaat cgcgaaagct atttgcgcca
    15001 atatcatgct caacaaaaac aaatcgaaaa gaccgaagct tatattcgaa aatacaaagc
    15061 cggttcccga tcaacgatgg caaaaagccg acaaaaacaa ttagataaaa tcgaacggct
    15121 gacgcctcca gcttcagcaa gtaaagctgt ctttgatttt ccattctcgc ctattgtcac
    15181 gacattagca gtggaaacaa ctgatttagt catcggatat gatcgaccgc tacttgcacc
    15241 catcaatttg accatccgct ttggtgaaaa tattgccatt cgaggcttca acgggattgg
    15301 aaaatctact ttattaaaaa cactgatcgg tgagattgaa aaaataagtg gcgatttcca
    15361 cttcccagat aacacaaaaa tcaattactt ttcacaagat ctgtactggg aaaatccatt
    15421 agaaacacca ctacaatatt taagcagtca atttccaaaa gcgactatca aagaattacg
    15481 tcgccaacta gctaaagcag ggttagttaa tcaattagca agtgaacctc tcactacatt
    15541 aagtggtggg gaacaaacta aggtgaaact tgcacagttg acaatgaacc aaggtaattt
    15601 actgattctc gatgaaccga cgaaccatat cgatcaagaa acgaaagaaa gcttacaaga
    15661 cgggatacag aaatttccag gaacagtaat catcgtctct catgaacaag aattttatca
    15721 agatcttgtg caacgagtca ttgagattga accaaaataa acgtttgatt gaatagttga
    15781 aaaaaaggag ctgtgacaaa agttttgtca caactccttc ttctgaataa atggtggcca
    15841 attagctgac cttcggtatt agctcaaaaa acggcaaaat atgaggagct atttttattt
    15901 ggctattctt attacttgat gcagaacagc ttttcacaac ctcttttcgt ctatttttta
    15961 ttttcgagat ttctctttcc tttaaaaaca actgtttgaa agaagaacgc acatccttga
    16021 aaaacaagcc cgctaaccgt tacacctaac cagccatact gctgccacat tgtcgtccct
    16081 agcaatgaac cagccgcacc tccaataaag tacataaaca taaatacagt attatttcgg
    16141 ttgcttgctg cttcacctag cccctgtact cgtgtttgat tggcgacttg accaaattga
    16201 ttcccgacgt ctaataaaat aatagctaaa agcaaaatgg ccaaatggct tccgccaacc
    16261 aataaagcaa taaagcttat tgtttgcata acaagtccca tatacacgat ttttctttca
    16321 gaataagaat ctgccagtcg tcccacgata ggtgcagcaa ctgctccaga caaaccaaaa
    16381 atcgctaata tccctgcttc gaatgttccc caattgtagg cgggactgct gatataaaaa
    16441 atcaacgtag accaaaagat cgaaaatgtt ccaaacatga aaaatccact gactgccgct
    16501 tctcttaata gacgttgaga agcaactaaa tgcggcaaac ttttaagtga atcaaggtat
    16561 gaaacagctg tactactttg ctggacagaa cgcggcattt tccagtaaag cagaagtgta
    16621 agaattccga ctgcacataa agcaaataaa tagatcatcc gccaagaaaa aagactggca
    16681 acaataccag ataccgtccg agaaagcaaa atccccacga gtaaaccgct aagcaatgta
    16741 cccatgacct tccctcttgc ttttggtccc gctagtaccg caccgtaagg aatgatgatt
    16801 tgcggcacga tcgaaagtaa tcctattaaa aaagaagcgc atgcaaataa cgtaaacgaa
    16861 ggcgcaaaaa aagcggccaa cagagagacg gcagataacg ctgccattcg aatgatcaat
    16921 tttggacgat ccaccacgtc ccctaaagga actaaaaaca gtaaccctaa cgcatagcct
    16981 aattgggtca acatcgtcaa tatcccgact gcacttgtgc taacagataa actagctgct
    17041 acttttgttc caatcggctg gatgtaatac atattggcaa caacaacgcc acaagtgatt
    17101 gataatagta aaataagcga agttgttaaa ggtttttttt cattactctt cattgatttc
    17161 ccccatttcc tatcttagtt tacaacttat ggaaaagatt ggaaatagaa aaatacaact
    17221 attcgccaca cttattattt gtgcttttgt tttccttgtt ttttcttgcg atctttataa
    17281 atgaagacaa ctaaccctac tagcacagta acacttgcaa ccaacgtgac tgcagttttg
    17341 ttatatccgt gaaacagagc ttctccacca cacgcataca acacagacga agggatgact
    17401 ccaatcacga tcgacaatat gatttgctgt agaggcaatt tcatcgcatc tgcagcaaaa
    17461 ttgaccatag aagatggaat aaccggtatc atatagccta acattacacc tatttttgga
    17521 tgtttcattt gtcggattgc ttgcacccaa tgattcgttt cggttttctt atctaaaaat
    17581 tttaagcgat gcataaggaa aattgcagtg atgttaccaa gagcattacc caaaatgttg
    17641 atgatcgcac ctgtcagtgg tccaaagctc aaaccagcaa tgactccaac tactgaagta
    17701 ggaatacctg gaacagcaca catcaccgct gtcaatacga tcaagagtat cgatgcaaaa
    17761 gtcccatgcg aacgaatatg tgcgagtagc tctgccttac tcgctctcgg atttaaaaat
    17821 aattggatgt ctgtctgata ttctaaatac aaccgatagc ccactaccag tatcagaatg
    17881 attcccaacg agatcaatat caatcggatt attttctttt tcatatttcc tcctgaaaaa
    17941 aatctatatt cattaaataa tagtttaaca taaaaacaaa aaagcaccga aatccaacat
    18001 aggatctcag tgcttttaaa tagtaaaaat tatttaacag ttacagatgc gccaacttct
    18061 tcaagtttag ctttcaattc ttcagcttct tctttagata cgccttcttt aactggtgca
    18121 ggagcgccat caactacagc tttagcttct ttcaagccta aaccagttgc ttcacgaact
    18181 gctttgataa ctttaacttt ttggtctcca gctgcagtta attctacagt gaattctgtt
    18241 tgttcttcag cagcagcagc gccaccagca gcagctacag ctacaggagc agcagcagat
    18301 acgccaaatt cttcttcaat agctttaact aggtcgttaa gttctaggat agttgcttct
    18361 tttaactcag caacgatgtt ttcaatgttc aatgccatta taatttcctc cgtttaattg
    18421 ttctttttta tttaatgctt tatgcggcaa ttaagctgca tctccatctt ctttgtctgc
    18481 cactgctttg acagcgtaag ccacgttgcg gactggcgct tgtagtacag atagcaacat
    18541 agatagaagt ccttcgcgat ttggcaattt tgccaatgct gtgatttctt ccaatgaaga
    18601 gactttacct tcgatgatac cgcctttgat ttctaatgct tttgcttctt tagcaaattc
    18661 gtccatgatt ttcgctggtg cgactacatc ttcgttactg aatgcaacag ctgtaggtcc
    18721 agtgaatact tcgtccatgc cttcaaggcc agctttttta gctgcacgtg aaagaataga
    18781 atttttgata actttcattt ctacgttagc atcacgaagt tgtttacgta aacgtgttac
    18841 ttcatcaact gttaatccgc ggtagtcaac gactactact gatgcagctg ctgtaaattt
    18901 ttcgcttact tcatcaacga ttgctgcttt ttttgcgata attgcttcac tcatttttga
    18961 gtttcacctc ctgaattttg atggaagagt tttttgcact gaaaaggcaa gtcactttta
    19021 tagttacttt gctcaaccag tattattcat cacctattca cttcgttcat agtgattcta
    19081 ctcgctttgc tcgaaaggaa actcctttcg ctttgctcaa ccagtattgt tcatcatctc
    19141 ttcacttcgt tcatagtgat tcacaataaa aaactctatg ccaccgcaga catagaggga
    19201 ctattgaata tattcaataa acgtcctcgg taggaaatta aggcttgatg ccacctactg
    19261 tcttcggtac aagttaatta ttaacctcaa ctagcttagc atgctaaact agttgagtca
    19321 agaattattt ttcagttgga tcaatcaaca ttctaatatt taattagaaa gaagcttggt
    19381 caacatggat accaggtcca aatgttgttg tgatcgttac atttttcatg tattgacctt
    19441 tagctgctga tggtttagct tttagcaata catcatggat tgctgcaaag ttttcaacta
    19501 atttttcatc gtcaaatgaa acttttccga ttggtacatg gatatttcca gctttgtcca
    19561 cacggtaagt tactttacca gctttcactt cgtttactgc ttttgttacg tccattgtaa
    19621 ctgtaccagt tttagggttt ggcattaagc cttttggtcc taatactcgt cctagacgac
    19681 ctacagtagc catcatatca ggtgttgcaa caactacatc aaagtcaaac catccgtttt
    19741 ggattttttg aaccatgtcg tcgtcaccta cgaaatctgc accagctgct tcagcttctt
    19801 ttgctttgtc tcctttagca aaaactaaaa cagtttgtgt tttaccagtt ccgtttggta
    19861 atacaacagc accacggatt tgttggtctg cttttttagg gtctacgttc aaacggtatg
    19921 caacttcaac tgttgcatca aattttgcaa tgtttgtttc ttttgctaaa gctactgctt
    19981 cggcaacagg ataaacttta cttgaatcaa ctttttttaa tgcatcttgc atttttttgc
    20041 tctttttagc cattttggtt cctccttgat tgtggttata acggttgtac ctcccacgtg
    20101 ttctatatct ttcaatatag agccaactga gaagctgctg tcttagggtg tatagattat
    20161 tctacagtga tccccatgct tcgtgcagtt ccttcaacca tgcgcattgc tgcttcaacg
    20221 ttagctgcgt ttaggtcttc catttttaat tcagcgattt ctcttacttg atcgctagaa
    20281 actttcgcaa ctttattttt gtttggttcg cctgaacctt tttcgatttt agctgctttt
    20341 ttcaatagaa cagcagctgg cggtgttttt gtaacgaatg tgaatgaacg gtcttcgtat
    20401 acagaaataa caaccggaat gatcaaacct gcttgatcag ctgtacgagc gttgaattct
    20461 tttgtgaatc ccatgatatt gatacccgct tgacctagtg caggacctac tggtggagct
    20521 ggagttgctt tacctgcagg aatttgcaat ttaacgattt tttctacttt ctttgccacg
    20581 agacatacct ccttaagtcc gtgatgtggt taattggaga tgcatatttc tcctcccacg
    20641 ttttatctga tctgaaaaaa tccagacaaa taatgtgcaa aacaatgcac actggtacag
    20701 tatagcaaat ttatttgttt tttcaagatt tttttctgct atttttacat tttgcttgaa
    20761 taaacgctaa atttattcta aaaaaacaac aatttttcgt tctcatttct tcttttatgt
    20821 tacacttgcg ctagcatggt tcaacagatg aaagcaaaca ataacacggt tttttagacc
    20881 cttgtacaaa gattcctaca taacaaaatg aggtggagaa aatgaaagat ttaaggtata
    20941 gaagtgtctt tgatattatt ggacctgtga tgattggtcc aagtagctct cacactgccg
    21001 gagctgctag aatcgggaaa atcatacgta gtatatttgg cgaacagcct gattctgtag
    21061 atatttatct atatgaatca tttgcaaaaa cctatcgggg gcacggaaca gatattgcat
    21121 tagttggcgg cctgttaggg atggaacccg atgatgaacg tttagctgat tctttgaaat
    21181 tggcatatga agccggtatg gaagtcttgt ttgttcctaa gagtgaaaaa gccgaacatc
    21241 caaactctgt aaagatgctt ctaaaaaaag gaacacgaaa gctttcagtc actggcattt
    21301 cgatcggcgg tggcaatatc cagatttctg aattgaatgg attcaaaatt tctttaagca
    21361 tgggtactcc taccttgatc gttgtccacc aagatgttcc aggtatgatt gctaaagtga
    21421 ctagtatact ttctgagacc aatacgaata tcggcactat gacagtgaca cgggaatcaa
    21481 aaggtgaaaa tgccattatg ataattgaag tagatgatcc tcaagtagaa gaaacggtaa
    21541 aaagactcaa acagctgcca aatatcgaca gcgtaaatta tttcgattaa ggagctaaaa
    21601 aaatgttttt atcgattgaa gaattagtag atcaagcaaa agcttatcca agtgtagcag
    21661 aattgatgat tgcagttgaa atggaaatga gtggccgttc ccgtagtcac atcatcgaga
    21721 tcatggaaaa aaacctctca gtaatggaac aatcaatcaa cgagggaaaa gaaggtgtta
    21781 cctccgtcac aggaattacc ggtggtgatg caaaaaaatt agatgattat ttaaatgagg
    21841 cagacttttt aagtggagaa acaatcttaa aagctgtacg caatgctgta gctgttaatg
    21901 aagtcaatgc aaaaatgggg ttgatttgtg ctacaccgac tgccggaagc gcgggtgttg
    21961 ttcctggtgt gctgatggct gtccgagatc ggcttgattt gacacatgaa cagcaattgg
    22021 aattcttatt taccgctggc gctttcgggt tagtcatcgc aaacaacgct tctatcagtg
    22081 gtgcagaagg gggatgtcaa gcagaagttg gttctgcaag tgcaatggca gcagcagctt
    22141 tggtttgtgc aaaaaatggg actcctcatc aagcagcaca agcagtagcc attacattga
    22201 aaaatatgat gggactgatt tgcgatcccg ttgcaggctt agttgaagtc ccgtgtgtca
    22261 aacgtaatgc tttaggctct tctcaagctt ttatctctgc tgatatggca ttagcgggta
    22321 tcgaaagtgt cattccgccg gatgaagtcg ttgctgcaat gtaccaagtc ggacgtcaaa
    22381 tgccgtcgat tttcaaagaa acagcagaag gtggcttagc tgttacgcca actgctaaac
    22441 gacttgcgga agagatttta aataagaggg cgtgataaag ctccttaatt tcgagtatta
    22501 agagattagt tatcttcata acaaaaaacg aagagaggac taattgccct tctcttcgtt
    22561 ttttattaat ccatcaattt cgtatatttt acttcatcga tcgtttttgt tcctgcaagt
    22621 tgcatcgtga tcgacaattc tttgttcagg tgttcaaaaa cagatgttac tccttctgcc
    22681 ccacctagat tcaacccata aatcactggt cggccaatcg caactaagtc agcaccactt
    22741 gccagagctt tgaagacatg ctctcctcga cggacaccag agtcgaaaat cactggtaca
    22801 cgtttgttga ctacttcggc aatttttggc aatacttcaa aggacgcagg acctccatct
    22861 agttgacgtc caccatgatt ggatacccag ataccatctg cccctgcaga gatagcgatt
    22921 actgcatctt ctggcgattg tattcctttt acgataactg gaagatgggt gatttctttg
    22981 attgttttga tgtcatctgg tgtcaagcct tgttttgcag ctgcataaat ttctgcgatt
    23041 ccttttcctt cgccattacc acttgcactt tgttcgctat acgcagctaa gttaggcatt
    23101 ggtaatggaa attggaattg attgatgacg tcttcttcac ggtaaccacc gagcgttgaa
    23161 tctgccgtta aaatgatagc tttagcccct gcttctactg ctttgttcaa aatgaactca
    23221 ttgaatctgt cgtctttact catatataat tggaaaaact gcggtgcgcc tggcgctgca
    23281 tcggctgcgt ctttgattgt cgtatttgca taagtgctga tggaaaagat tgatccagca
    23341 gcagctactc ctttagctgt atcagcttcc cctttttcat gtgccaatcc ttgggccgct
    23401 gatggcgctt ggataatcgg tgtatctaat tcaattccga aaacagatgt atgcaaatcg
    23461 gctgagtcga ttccgcgtaa tatccgtggc atgattttct ttgtattaaa agaagtcgta
    23521 ttttctttca tcgtccactc atcttcagaa ccaccgcgaa tatagccaaa agctcctgct
    23581 tccatgcgct cttttacacg accttctagt taggcaatgt ttacaatatc gattggatgt
    23641 tcttcattac ttgtatgata agtcatagta gatccttctt tcatcttctt gattacatat
    23701 aaatcatact aaacttacaa aaagtaagca aacatttact ctcagatact tttatcgcta
    23761 ttctttccaa ataaatcgac ttacaagctg atctacttct tggttttcat gaagcatact
    23821 atgagaggca ttttcacctg tcactacttt ttcctctatt tcatttccgc gttgcttcaa
    23881 caacgcatag accgctaatg cactgtttaa cggtactgtt ccatcactca agtctgtttc
    23941 atctagttga ccagcaatca agaaaaatcg agtggttata ggaatattac cgatcatttg
    24001 ctggtaatct tgatagcggt tagaaacgac agcaggcccc tttgctaact catctgtcag
    24061 agactgcgca ctatcatcga caaaatcatt aaaaggcgcg ccaattgctg ttaatgtctg
    24121 aacttgtggc aatgacgtat cctgtccata cgttcctaaa tagcgtagtg cactaactcc
    24181 tcccatggaa tgccccacta tgttgacttt atctatgccg tagttttctt tcagatacaa
    24241 aagacaagtt ttgatccatt ctgcttgatt ccactcgtta tttttattat cttcaaacaa
    24301 tacttggacc atcggattgt ctgcttttcc agacaaatct cctgttgctt gtatttgtcc
    24361 atctgttcct actgttaaaa ccaattcttt tactgcttgc tgattttttt ccaaacgccg
    24421 caacatccct ttaaaggaat tttctgtccc gctgtagccg tggaaaaaaa gtgttggaat
    24481 agtggttttt tctatcactt tactggattc tgccgttttt tctttatcag aaacttcagt
    24541 agtatttatc ttcgtcgtct ggcaaccact taacgcaaac aaactgataa ggactaaagc
    24601 tgctgcagtt gcacgtttgc ataaattttt gattggttct gtcatacacc tcttcctttc
    24661 catatagaaa aaagaaagac ttgaaaggcc aatcatatga tcgacatttc aagtctcttt
    24721 attaatcgat tttatctact tgatcgaaat caagttctgt actggtttca cgtccaaaca
    24781 tatcgatgtt gactttcaat ttttcttttt ctccgtcaac ttctgttact tgtccttcta
    24841 atccagcaaa tgcgccttcg atgattttca cgacttcgcc aacttctact tctagatcat
    24901 tttgtcgcag gctcataccg atagaacgca agatatgatt gacttcttct ggaagtaatg
    24961 gtgctggttt gcttccggct ccgtgtgaac caacgaatcc tgtcacacca ggtgtgttgc
    25021 ggacaacata ccatgaagcg tctgtcatga tcatttcaac aagtacgtat cctgggaatg
    25081 ttttatgaac gatttctttt tctttcccat ttttgacttc agtttctttt tcttcaggaa
    25141 caacgacacg gaaaataaag tcggccattt tcatactttg cgcacgagat tcgatatttg
    25201 cttttacttt gttttcatat cctgaatatg tatgcaagac ataccagttt ttttcaaaag
    25261 attccattga aggctcctca tttcattgta aaataaaaaa acctcagtac acccgaagtt
    25321 tttctttcgc ttcattatag cactaaaagt acgttgtggc tagcgagtta ttttaaaatc
    25381 cagccaaata agctttggat ggctgtatcc attacgtaaa acatcaatcc gaaaataata
    25441 gaagtttcga tgactactaa cgtatcttta cggagttgtt ttctgcttgg ccaagttact
    25501 tgtttgattt catcttttac gctttttaga aatttcatga gacaacctcc cttgctactg
    25561 tttatttcgt ttctttatgt aatgtatatt gattacaata tttacaaaac ttattgattt
    25621 ccaaacgttc gccgcttttt ccttcactga ctgattttgt gtagttgcga gacccacaaa
    25681 cggaacaagc gagtgctgct tttttctttg ccatttactt ctgccccctt gctgagaatc
    25741 agacttatac ttcataacag tatcacgtaa ctttttactt gtcaatttgt gaaataaact
    25801 atctattgtt ttggataaaa ttatgatatg attaatttga aattaagcta aaatgagaca
    25861 attggaggta tagcgatgct attaaaaaca cttgtatata agaaaaaaga tctgacaacc
    25921 gttacagaga ctagtacatt agaagaagca ttaaaaattt tagaaacttc cggataccgt
    25981 tgtgtgccga tcttagattc ttcaggtaat attttccgtg gaaacattta caaaatgcat
    26041 atctatcgtc ataaagcaaa tggcggtgat atgacgcttc ctgtcactca tcttttgaaa
    26101 aacgctacaa agttcattta cctggatact tctttcttca aagtattctt tacgatcaaa
    26161 gagcttcctt atattgctgt ccttgacagt gaaaatcatt tttatggtat tttgacacat
    26221 agtacgttgc ttaatatgct ctctcaatct tggagcgtag aacaaggaag ctatgtactg
    26281 acaattgctt ccacaggaaa gcaaggagat ttggctgcta ttgcaaaaat catttcaaaa
    26341 cacagcagca ttgccagctg tattactctt gatatcgaaa aagacgaata cattcgacgt
    26401 acattgatca cgctaccggc taacactgaa gcgaaagtat gtgaaacgat cgtcaaccac
    26461 ttagagaaaa agaattataa agtggtagaa attgaaaatt tgcaaaacca agaagcataa
    26521 tcaaaaagcg aaaaaagaaa acagctaatc tgctttcttt tttcgctttt tataattaga
    26581 cacgcttttc aaaatcaagc gcgcgatcta aataatacat caatggtgct gaaacagcca
    26641 tgacgataaa ctcacttaat gcaagcgtgc cataggttgt ccaaaatgct tggccacctg
    26701 ttggaactaa catataagca atcagtccca tgctgatggt aaagaagatc gtgttcaata
    26761 ctaaacgcat ctttacattt ttcactttgt tttgcaataa agctgtcagg cctaaagaaa
    26821 gcaatgtttg gccgccacca aaaaccacat ccatgatacc aaaaccaaag aaggcattat
    26881 agacgatgac cccgcctaaa actccccaaa gcaatttacg gttaaagaca actaaatgat
    26941 tcaaactttc tgaaatacga aactggatcg gccctgagga tacaggcgca actaaaatag
    27001 ttaacgctag ataaagagcc atgatgatgc catttacaac gattacggat aaccgttgtc
    27061 tgcttttcgt tttttgcatt ttctctcctc ttttctccta gtttttttaa cgcgggatgg
    27121 ttgatgaacc gcggttatgt gaaacaaaca ataattataa cagacttttc atttatttca
    27181 atccgtctta tctgctttta aaaaagcaaa agcttaaaca atgtaaacgt ttgctaaaaa
    27241 tcgtgggata tggtataaag taagtgtaag gggtcataag aattcccagt aaagcagacg
    27301 aagtaggatc tgcaagaaca gaattgttgt gaaacttaaa taaataaaaa aggatgtggg
    27361 aatttgagca acctcttact agagaacggt ctagcaagca gctgaagatc taagtggaca
    27421 tgccgaacct tggaacttct ccttacgtaa gtaatggatc atgcgcttgc taggcttttt
    27481 tgtgtttttc ggtcttcgga cctttttcat tttcctagac ttctgctata ataaccaaga
    27541 attaattttg taacagattt ttttgaaaaa acaagtttct ttcattcaaa aaatagaaaa
    27601 agcttgctaa ttattggtta accagcaaac aggagtatta aaatatcttg ttttattgaa
    27661 tagcaggagg aaatcatgaa attccattat catatattga tcaatccatc ggcaggcagc
    27721 ggaaatggct ataaagtagc agaaagaatt ttaccggtat taaaaaataa gcatattgac
    27781 tatacacttc attatagtga atacaaagga cacgatgctg agattgctga aactttggca
    27841 aaagaaacat tgattccttg gatagaagaa gaacagaaaa caatcgatac tttccccctt
    27901 ttgatcatcg taggaggtga cggcacctta catcaggtac ttgatacttt ctatcagatg
    27961 gaagtagaat tcccagttgc ttatattcca gcaggttctg gagatgattt tgctcgaggg
    28021 gcaggccttc caaaaaatcc caaaaaagga cttcagctga ttttagcagc tcaatcacca
    28081 gaaaaagtcc acataatggc ttatgaagaa aaaataagtg aaaaaaaagg aatagctgtt
    28141 aataatttcg gtataggatt agatgcagct atcgtccatg cgacaaatca ttcttccaca
    28201 aaaaagcgat tgaacaaata caacttagga tcattttcgt atttattctc tattctccgt
    28261 gctttattca ctcaaaaagg atttccaatt ctggtagatg caggaggaaa acaattaagt
    28321 ttttcaaacg catttttatg tacagcaacg aagcatcctt tttttggcgg aggaatcgcg
    28381 attgttccaa ctgctgatgc gtctaaacca gtcattgatt ttgtcgtggt cgaacgaatc
    28441 aatttcttta aaatcttgtg gctcattctt ttacttatac agaaaaaaca attgaaatcg
    28501 aaatatttcc atcactacac aacaaacaga ttacgtatcg tttcaacaac accgcaacat
    28561 ggtcaagaag atggagaaga aatggaaaaa cgaccttttg atatcacaat aaccacaact
    28621 aaacagttgt tttggtgtta ataaaagagg ttttgtcaga agctacatct ggaacaccgt
    28681 ttattcggaa aaaaggactg tgacacgact tttgtcacag tccttttttt actatgcagt
    28741 taattctttg cccaattggg ttgccatcga tagtttcttt ctttacccaa aataaattgg
    28801 atcattgaaa acaccgcagc aaaagcaact aagacacaac cattgaacca aaaagattct
    28861 gcaaatgttt gattgtaact gagtgtttca ctgatcaagt tattattttg aacaaaccaa
    28921 tatgttggtg tgaatgccgc tattttgcta accatttctg gcaagtattc tgctggtaca
    28981 aacaccccac cgataaagca actgcctgtg ataaaaatat ctttgatccc accgctggtt
    29041 tctgagtttt ttaccaatga agtgatacaa gccgaaaagc taatgatcgg caataaaaac
    29101 aagaaagtat ttaaaatatg ataccatacc gttatatccc acgaggtgtg gatcactaac
    29161 agaaccatta gaagtaagaa gccactgaac aacagcagcg agtaactaat caaactaaag
    29221 gttactttgc gattcaagtt tcgtttggta atgggcgcac tctggtttcg tttactgatc
    29281 tctttgcgat taaatgctag attgatgacg ctgaaaccgc taaaaatgga tagaaacaac
    29341 ccataagaca agaaattaaa ggttgtgccc gtcaacagct ttttcatccg ctgactatat
    29401 cccgcactta gctcaacttc tccagtaacc gagagtgcca tttgtgtctg ttccatagct
    29461 gcatcgattg ttttatcttc ttcttgaaac agaatcagtg tttgcaaaaa ggtgttcact
    29521 tgctgtgtaa caactgtttt agaaaaggca tctgggcgac tctgtatctt gatcgtcggc
    29581 atctttccta cttcgacttg ttcggtaaag tctgcaggca gatgtaagat aatgtttaca
    29641 cgattaaaat aaagagcatc atcgatttct ttttgtgagg ttccatccaa tgtaacgact
    29701 gtttccttgt cattcaaata tttacgaagg ccatcgacta caggcgactc ttcttcacta
    29761 atcaaggcga ttttcgcatc ttgcagagag gtattttcaa catcaatatt ttgcgcaaaa
    29821 aagaatgtgg caacaacagt gataattaca cctatcagta aattcgattt atttgcttta
    29881 agcaatgtcc aaatcgtttt ataaagcgtc ataacgcacc cctctttcca tccaagcatt
    29941 tcctaacgca aaaaggaaaa taaatccgat caaccataga agattggtat aaaaactagc
    30001 caaatcagtg tagaaataaa gtttatacaa actttcgctg atcaagttga cgagatttaa
    30061 ttgaccaaca atcggaatat gaagatcaat ccaatacttc acttgttccg accccatcat
    30121 accagcaaaa aagctcatcg caatcgtcac tgaaatcaag atgctatcct tttgcaccag
    30181 attcattttt ggtatcaaat taccaaccaa tgtacctaac aaaatcgcac acaacgaccc
    30241 cagagcggta acaatcatca accattgcca acgttcacca aagtctactt gatagataaa
    30301 acgaaagaca gctaacataa tataaatctc tgtcaaaaca atcgtaaagc ttgctgccaa
    30361 attagcgatt gaaactaacg attgctttct aggaatcaag gacaagcgaa taccatttga
    30421 cgattgattt gcttgctgat cctgtacatt gcggaccccc cacatcgtcc cgtagagaat
    30481 cgccattcca atcagagtga agaaataaaa acttttgaaa ctcatattac gacctgcatg
    30541 aacttcttga aaagatactt cttgttcaaa aacagtcatg atgggcgcga gatcaacgtt
    30601 ctccacttct gccactttac tcgaaattat accaatacgc tgtaaaaatt gatccatgac
    30661 gtttttcatc aacgtctgac tcattcctgc ttgacccaca tggagcgaaa cagatgaacc
    30721 atcaatgaga taaaaaccag ccaaatcatc tgcatttagc ttttcattcg cttctacttc
    30781 cgatagttcc tgtacttcaa ataatggttt atcattgact tcgacttgtt ctaagactgt
    30841 ggtaaaattt tccgaaagcg ctgtttgatc cgctttgatt acaattccag tattaatcgt
    30901 ttcgagattg ccaccttgat caatctcgcc aaaggccgcc ataaacatca atcctaaaag
    30961 aattggaaag gccaatgtcc aaaacaacat ggatttattt cgcagcagta tttttgtgcg
    31021 atatttataa aaatgccaaa acattatttc ccctcctcta gtctctcaaa tccttgccag
    31081 taatctctaa aaagacatca ttcaaggacg cttctttggt tgctattttt ccgtaactga
    31141 tcttttcttc ttgtaagatt gtaagaatcg gcaacaatac tgtatttagt tcagtagttg
    31201 ttacttggag ctgacctttt ttataagcga ttccatcaga agctattgtt cgctgacaac
    31261 acgttttctg ggagtaatgg gatgtcgatc gtcaaacgat ccgccgtccg taccatatct
    31321 tttagagatt ctttggttcc tttagcaatc acctgtcctt gatccatgat cacgatctgg
    31381 tcgcaaagaa tttctacttc ttccatgtaa tgtgttgtat agacaatggt tgctccttgt
    31441 cgattcagct ccttgatact ttctaaaatt ttattccgac tttgggggtc tacagctact
    31501 gtcggctcat ctaaaaaaat taaccgcggc ttgtgggcaa tcccacatgc gatgttcaac
    31561 cgacgtttca agccaccact aagttctcct ggacgaaacg aaacatattt ttctaatccc
    31621 actaattgaa tcacctcttc aatcaaggct ttccttttct ctttttcgga aacatataaa
    31681 ccgcaaaaat aatcgatatt ttcttgcaca cttagttcgt caaagaccgc aacgtcttga
    31741 ggaacgaccc caatcattgc cttgacagca taattttcag gagtcattgt tgtcccgaac
    31801 aatttaatgg ttcctttgtc ataatttaac aaagaaagca tacagttgat ggcagtcgat
    31861 ttcccactgc cgtttggtcc taacagacct aagacttctc cttcttgtac ttgcagatca
    31921 aaatgattca atgctacaaa atctccatag cgtttgacta atttatcaat ttcgataatc
    31981 atcatgaaaa actccttcca aatttcttgt tgatctgact ataataaagt gcaagaaaag
    32041 aaactagtga cgattggcta aaccacacat gacatttgtc acaatgaagg agtctgctat
    32101 gcataaatct cggctactca acgaaactag ctttctactg gttatctcat tgcttttgct
    32161 ggtaagtcaa ggattcgttg ctatgcaagt tatctattta ttgatcgccg ttttattcac
    32221 tggtgtcagt tatttactgc ctacaaagcc aaattggatt ttccaagtta ttctatttct
    32281 tttatgtgcc tttttacctg atcttgtcct gtttctgcca gcgacgatgc gagtgatcgc
    32341 ccctgaagaa agccaccttg attacttgtt tttgttaggt tttttgttga tttcgacggt
    32401 tggtaatcat ccccccatca aggtcgcctt tgtcgtaatt ttgctgtttt tttcctttta
    32461 cttacgaaaa aaagatcaac gaacgaccga tcttgagaaa aagtacattg tctccaaaga
    32521 tactcattgg gaaaaacaac aagagttatc ggaacaaaat aaagcattgg ccctgtcaca
    32581 agaaacacaa ttagccttgc aaattgtgga agaacggaac cgaattgccc gagacatcca
    32641 tgacaatgtc ggtcatctgc tgtcaagtgc gattttacaa attggtgcgc ttgaaatgat
    32701 caatcaagaa ccaaaactaa aagaaccatt agaaaattta agtgccaccg ttcacactgg
    32761 gatggatcgg attcgtcaaa gtgtccatga cctccatgag acatcgatct cttttcaaca
    32821 ctccttagag tttctgatcg ctgattttcc ttcttctgtt acgattgaag gaaacttgtt
    32881 tgaaggattg aatgaaacgc agcaaaactg cttcttaatg gtcatcaaag aagccttaac
    32941 caacagtatc aaacatagtc atgccaaaac cgttgtttta cattttcgaa cgttgccggc
    33001 cttttatcgc ttgcaaatca cgaatgatgg caccagccca caaaaaaatt ctgctcccgg
    33061 tattggtttg aaaacgatgc gtcaacgtgt tcaagaacaa aagggacaag tccatatttt
    33121 ccaagaaaac cagcaatttc agttaacgat cattttacca agggagggac accaatgata
    33181 acagtagtca ttattgagat gatccatttg tgacgaaatc gttacaaacc attttagaaa
    33241 gcacgaaaga gatccgtgtg ttaggcattg gtcattgtgc caaggatgcg ctagtcttat
    33301 acgaaaccca tcgtcccgat gttttgctga cggacatccg catgcctaaa caaacgggca
    33361 tcgatgctgc caaagaaatt ttggctaaat ttcccaaagc catccttttg cttttgacga
    33421 cattcaaaga tgaagaatac atccgcgaag cattttccat cggtgtcaaa gggtatttaa
    33481 tcaaacagaa cttgcaagcg attattcctt ctgtcaaatc cgcttataat ggacaggccg
    33541 tatttggtaa tgaaatcatc gagactttca ctcaaatgat gaagaataaa ccgacgattg
    33601 atagtcatgc ttatgcttct tttagtgaac gtgagcttgc aatcatcaaa gaagtagctg
    33661 ccgggaaaaa caataaagaa attgcagatg ccttgtactt gagtgacggc actgttcgca
    33721 attacatcag ccaattacta gaaaagctag atttacggga tcgcacgcaa ttggctatct
    33781 atttttatca gcatttgcaa taagtcttat ctaacacaaa aaaacctgaa tgatgctcct
    33841 tcgcatcatt caggtttttt tcacatcttc tattcaattg tcccgcaaac catttcctca
    33901 cgattgaaag aatcaaggat tcgcagggct gccaggattc gttggattgt tcggcgtgtt
    33961 aggcggaaaa ataggcgcct tgtctaacgt tttttctagt tttgattctt cacgatcgtc
    34021 tcgttctaca tcaggacttt cattcaattt gaattctttt tccatctcat ctaacttttt
    34081 aaagtcattg ccagattctt ttgctaattt cgctaattta tcttcggttt ctttagccat
    34141 aaagtaacgc cccccttttg atttcagctt agcatatcac actgaaaaaa caaaagaaaa
    34201 cgctaaccaa cgatcatttt tagctatttc tcatctttaa aatcaagcat tttttgccgg
    34261 tataaaatcg tagggagaac tgttttccat accaatcatc ggatctacta actggttatc
    34321 gatcacttct gctgtaagaa cgaaatcttt agaaacagca ggttctacct cttctttctt
    34381 ttcttctcca acaaatgaag aaaacagatc tggttccact gtttccatta cttggttttt
    34441 cgtttcattc gtcccactgt catcttctac aggtagcttt tcttttacaa cccgtggctc
    34501 ttttgtatag ttcccatctt caaatggatc atttccgacc aaagaatctt cataagctgc
    34561 tagtttggtc cggagagtga tattcatttt atcattatcc atgatccttg atttcaacgt
    34621 agtcatccgg cttgctgatt cattcgctac acaagtgaca taggcttgct cttcccctaa
    34681 caaaatcaac agttccttac tgcgagtcac tgccgtgtag agcaaattac gctgaagcat
    34741 ccgctgatat tgatggacca tgggcaaaat aaccatcttg aattcactgc cttgtgattt
    34801 atggattgag caacaataag aaagagtgat tttattccat tcgtttcttt tataagtgac
    34861 ctcatttgca tcaaactgga taactaattc atccacttta tcttcagaat cttttgccaa
    34921 gatgataccc gtgatttctc ccatatctcc attgaataca ttcaattcgg gagtattgac
    34981 taattggagg actttgtctc ctatccgata aacggtgtcg ttccaatgta cttccttttt
    35041 tccaggtttc cctggattga atatctcctg catcatttta tttaacgcat cgatccctgc
    35101 aggtccccga tacattggtg ctaatacttg gatatcttgt gcacggaatc ccttattttt
    35161 cgctttttcc acaattttag agatcaaggg ttcaatttga taagcatcac ttcggaaata
    35221 ggaacgatct ttttgattct ttgtgaaatc ttttggcaga cgtccttctt tgatctcatg
    35281 ggcaagagga ataatactag aaccatcacc ttgccgataa atctgattga gttccatttt
    35341 gggaatttgt tcgatttcca aaagatcatg gagtacttga ccaggaccga cagagggaag
    35401 ctgatctttg tctcctacta agataacttg catattcgtc ggaatcgctt tgaataatgt
    35461 gttagccagc catgtatcga ccatagacat ctcatcaaca atcaaaagtc cgccttctaa
    35521 ttctttcgta ctgactgaag gtgttttttc ccgtccattc agccccagaa gacgatggat
    35581 cgtactggca ggcagacctg tcgtttcgtt cattcgttta gctgctcggc cagttggtgc
    35641 tgcaagtaga ataggaaaaa tttcttgggt atattttgaa ggatccaatt ccagtccgtt
    35701 caactctgca aaaagattga cgattccgtt gatcacagta gttttccccg tacctggtcc
    35761 tcctgttaaa ataaatagag gggaacggat tgcttcagcg atagcttgtt cttgcgaagg
    35821 tccatactgt atgccgaata ttttttcgat acgccgaata ttcttctgca cttctttttc
    35881 aggataatgg atttcttttt tacgactgag aaggcgctgg atcgaattac cgatccccca
    35941 ttcagaaaag tacaaactgt tttcgtaaag attagtctct tcttgttgta tctttccttc
    36001 ttcaacaagt tggataatta cagtagcaac ctgatcaggc tggatttcaa ttgggcgact
    36061 tgtttctaaa atacggatcg tttgctgaag caatgtctcc gccggtacgt atgtatctcc
    36121 tgtttgaact gcatgctgaa aaatctgatg caagatagct gctcgaattc ttctcggtga
    36181 atctgctgca ataccgattt gttcggctag attatctgca cgtttgaacc cgattccttc
    36241 tatgtcttcg actagctggt acggattttc ttcgatgata tccaaagact cgttcttata
    36301 ggcctggtag atactgaaag ctaactgact accgaacccg taacgattca gtcccatgat
    36361 cacttgatcc atcccgtggt ttagccgaat cgtctcagca agcatttctc tttttggctt
    36421 cgtcaaaccg gagatttgtt ttaataccga aggatcagca atgatttttt cgatagcatc
    36481 ttctcccagc aattctacaa ttttttctgc tgttttcttc ccgattcctg ggaatttttc
    36541 gctggaaaga tacgctacga tcccatttgc agttgttggc ttttcttgct catatctttc
    36601 gagtttaagc tgccttccgt atttaggatg atccactaat tctccataaa aacgataagt
    36661 ctcattttct tgaatatcgc cgaaacttcc ggtgactaca atctcttttt cacggtaatc
    36721 agctgtggtt tcactgatgt gtaccaaaag cactttgtaa aaattactag gattttgaaa
    36781 aaaaatcgcg gcaactgtac caacgaaata aggcatttct tctggctgca tcggaagccg
    36841 cctccttctt ctatagtaaa ggttctggtg ctgtttcagt tcctaaaaaa tctgtttggt
    36901 tccatacttt cagttcatat ggcaataatc tgcaaggctc ttcagtctct aaaatcgtca
    36961 aactactatt gaacaatcca cccatttcac gcaactcact tagctctttt cctgtcatcc
    37021 attgaatggc tgcagttaaa gaagctccat gtcctacgaa taaaagtggg ccatctttat
    37081 gttgggcaac cgcatctgct acaacagttt cgatacgtgt gattgccgat tcaatcgttt
    37141 ctccgtgaaa aggagaagga tcatacagat ctaaatgatt acgcaaattc ggcagattat
    37201 ccggatattt ctggtacatt tcatcgatcg actgaccttc taaagtaccc agccctagtt
    37261 ctcttagctt atctgtataa aaaatctcta ccggacgagc aagctctttg ttgatacctt
    37321 ctgctgtatc acgtgctcgc tttgctgtac tggaataaat tgctttaaaa ggaacatatt
    37381 ttaccttttt cccaaatgct ttgatttctt cgtaactttg tggcaaaagc ggagaatcgc
    37441 cattgctccc ttgaaaacgt ctttcctgat tccattgtgt cttcccatgt cgggtaaaat
    37501 atagttccat acgcagcaaa ctcctgtctt tcttttattt tactctataa taaactgtct
    37561 aatccagctt gtcattttca tagtgatgcg tcaattctaa attagggaat ccttgctggc
    37621 gaagtgcttc atacactatc attgcagctg tattcgataa attcaatgag cggacatgct
    37681 catcattcat agggatgcgt aaacactttt cttcgttttc acgcataaat gtttctggca
    37741 aaccggttgt ttcttttcca aataaaaaga agtgctcttc tccatcagca aaattttcgt
    37801 cactatatac tttatgggca aattttgaga tcaaatgtag tttacgatta cctaaaaatg
    37861 ttaaaaaagc ttctaaatta ctgtggtaag taatatttac atcattccaa taatccaacc
    37921 ctgcccgttt tagatgtttg tcatcagttg aaaaacctag tggctcgatt aaatgaagcg
    37981 gagtattcgt ggctgcacac gtacgggcaa tatttccagt gtttgcaggg attaatggtt
    38041 caaataaaac gatatgattc atcaggatca ttcctctcat atttatgttc gtttctattt
    38101 tctgacaatt tgatttttgc ataaaaaaaa acgtatcaac tcggtgtcag tcactgcgag
    38161 tcgttacgtt agtgaagctc ttctcactaa cttttatcag aacaggaacc tgataaaaac
    38221 caatgaaaaa acaacttgtt gctaatataa caccctcaac ttttttttgc aacctctact
    38281 cattaaaaac tgctagaaaa acgttttctc ttagttcctt ttaataattc tatgtcaatt
    38341 gtaatcttct tcaaaggatt cttatataag tctgctttga ttcgctcttc tacctgccat
    38401 tctaaaggtg acatggccaa aagatctttt tgattttcct ctggtatctc aaattcatag
    38461 gttacacgct gacgttcagt taatttcatt gcttcagcaa acttggctac tacacgttca
    38521 ttggaataac tctgtttttc aggttgattc ggataaaaag cctgacgtag ttcctttaaa
    38581 taattttctt gtggcaccac cttcaacact tctccatctt ctgagagtac acgttggaat
    38641 tcttggtaat gagaaggcga aaagatattt aaaaccgcgt tgaatgtttg atcagcaaac
    38701 ggcaaattgg tcaaatcagc tacacaccaa aatgcttcgg cagattgttc tgtagctgcg
    38761 tagactcctt cttttgaaat atcaaatcca acagcatttt gaatcttttt caattttgaa
    38821 agttcattca agaagctacc ttctccgcag ccaacatcta atagcgagac ttcttcaggt
    38881 aaatgctgcg caatagtctc aataacgggc tgatacatgc cagattggat cattctacgc
    38941 cttggaacga acattccttg atcgtattct gttttaaccg catgggataa aaaataaagt
    39001 gttccttttt tggaaaggtc aaaacggtga ttatttttgc atatcaagcc attatcagac
    39061 ttttttactt cttcttggca aaccggacag cggaacagct gatgattttc tgccaaaaac
    39121 tggcgtcctc ggtcaatttt ttttaacata gttccccctc atttctgttt tatcttaccg
    39181 ttagatgctc actttggcaa ctgctaaaaa tatgatacaa tgcctagaag aaagtgaaag
    39241 ataaaggaga agattagatg atcaaagaat tttgcgctga aaactacaca tctattcctc
    39301 tagcaattgc tagaggagcc gatcggatcg aattgtgtga caacttagcg gtcggtggca
    39361 caaccccgag tactggtgtg atcgaagaaa cgttaagtta tgctggggaa aaagatatcc
    39421 ccgtaatgac gattattcgt cctagagggg gcgacttcgt ctataacgat atcgaattga
    39481 aaatcatgca tactgatttg attgaagcaa agaaattagg gacggatggc gtagtatttg
    39541 gttgtcttac tccttcaggc tggctagacg aagaggcctt agaactattg atcgaaacag
    39601 cagaagggtt gcagatcacc ttccatatgg cttttgacag tattcctgaa gatcggcaat
    39661 tcgaagcaat cgattggctg gtagaacatg gtgttgaccg tatcttgact catggcggtc
    39721 ctgctgacaa aactattgta gaaaatttcc cacggcttaa agaattgatt gcctatacag
    39781 atggccgctt aatcattttg cctggtggtg gtgtcactgc agagaatgca gcacatgtag
    39841 ctaaagaatt gaacgtaagc gaagtacacg gaaccaaaat cgtggatctt cagttataat
    39901 cttataaaca aaaaaacatt tgcaacaggc ataatgctta ttgcaaatgt ttttgttttt
    39961 ttacataaaa tagcccataa ataaagaagc aatattgaaa taaatcagta cactcgtcaa
    40021 atcgctcaaa gtcgtgataa agggaccgct tgctactgct ggatcaaatc ctagacgatc
    40081 cattagcatc gggattaaac tacctgcaag attcgccact gtgattgcaa atagcatagc
    40141 catccctatg acaaacccca agataaaatt atgtttccaa aaacctacga ttagaaaaat
    40201 cgtgatcccc gtaacagctc ctgtgacaac gccggtcaag acttcagcca aaacaagtcg
    40261 aacaaatcca tgatctttct catcagaaat cgcaagtcga cgaacagcca cagctagcga
    40321 ctgggttcca gcattaccgg cagttcctgt aatcagcgaa atgaagactg ctaaaatact
    40381 cgcttcactt accaagtctt catagtgact gattaaagaa gcggtcgaca tacctaaaaa
    40441 caacagagtg atcagccaag gcaaacgtcg agacgctgcc tttactggat tttcattaat
    40501 ttcttccaca ttgaccccgg caagtcctga ataatcgcta gcagcttcat catcaataac
    40561 gtcgatgata tcatcgactg tgacgattcc taacaaatga tcatcataat cggtaacagg
    40621 aagagctaag aagtcataat ctctaaatgt ttgtgccaca tcttcttggt cgtcgcctac
    40681 atgaactgaa agcactcgtt cgctcatcag atcagaaatc attgcgtcgt catcattcac
    40741 gattaggtcg cgcagagaaa taacccctac tagctgacct tcttgattaa cgacatagat
    40801 gtaatagatt gtttcagcta catctgcttc gttcttcagc acgtacatcg cagagcgaac
    40861 agtttgattg gctacgatcg aaacaaattc agttgtcatg atcgcgccag ccgtttcatc
    40921 ctcgtaatgg agaagttcct tgatttcact ggcatcgtct gccgacatca aacttaaata
    40981 tttagcaatt tgctttttat ccagcgtatt aagcaagtca acagcattat ctgtatacat
    41041 ctcagaaagc atatcagctg catagctagg acgcatttca tctaggtatt ctttcatatg
    41101 ttcgttgtct tcttcaatca catcgaacat atccgctaat tctttaggcg acagatagga
    41161 atagatcagc tgcctgttct cttctgtaag agattggtag aattgcccct gctcatagat
    41221 atgcatctct aaaaaactgt ttctgaatgc ctgaatatta ttttccctta aatcttgagt
    41281 caactgggca aatctttcct ctaattcttg attttcatcc aaaccaatct cacctcgcct
    41341 tattcttcaa accgtctcac aactgcctcc atgtctgcgg ctaaaggctg cttgaactcc
    41401 agcaactctt ctgtaaatgg atggtaaaaa cgcagatcat aacaatgaag ggcttgtctt
    41461 tttattccca gatccattcg cccgccatac atatcatcac cgagcagggc acagccaatt
    41521 gcggaaaaat gaactcttat ttgatgtgtc cttcctgtat gcagctggat atcaactaac
    41581 gcgatttctg ttagtcgctt ctctatccag tactcagtct gtgccgaacg tccttcatca
    41641 gagaccattc gcttcaatag cgaagaataa tctcgggcga tcggcaaatc gatatgtcca
    41701 tgtgccgata aatctgaaag ccttccactg actaaggcct gatatttttt cacaaacttc
    41761 ttttcacgaa gttgcatatc taattttgcg tgcgcaaatc catgctttgc aaaaagcatc
    41821 aaaccagatg tatcccgatc tagtcgcgtc accacatgga ttacttggtt ttcgtaacct
    41881 tgttttttat aataagcctt tacacgatta gccattgtcc catttggatg gtattgggca
    41941 ggaatagacg aaacgcctgc tggtttattt actaccagaa cgtgttcatc ttcatacacg
    42001 atatcgatcg gtgtttcatc caaaagtacg gtttcatgtt ctccttcagc aggaatcaca
    42061 atggttacct tatcatcctt agctagagaa aacaagacat tttctacttg atcattaact
    42121 ttgatttgac cgccttgaaa ttttattttc gctaacagac cctttgagat ccctttttct
    42181 tttaaaaaat attttacttg ctgagaatga tctttctcat aatcccatgt aaactgcata
    42241 tcaatcctca ctaataaagg catctttcac acgatgccaa aaatgcatat gtcgataaga
    42301 agcaaaatgg atccgttctt ctgctattcg ataataaatt gagcggatat ttgcttttgc
    42361 gacattcaat tgatcaaccg ttacaagata atcttctgta ttttccaatt ttatttctag
    42421 ccattcatca tgggcaatca cgatcggaga acccaaagta cgaaacactc gattattcag
    42481 tgaagcaatc tctgccaact gaaaggcatt gatactagga tggatcacag caccgccgac
    42541 acttttatta tatgctgtcg aaccagtagg cgtagaaatc gtcaatccat ctcctctaaa
    42601 actttcaaac agctcgtttt tgatgtatac atcggctacc atcgtacgat tggcccgttt
    42661 aatcgttgat tcattcagtg ctaaaaaatg ctggtccggt gtttcatcca aataactgat
    42721 ccgaacatcc agcaagggat aactaacact ttgttcccgg ttcgttcgta agctgtctac
    42781 tagttcttcc agctcgtaat cccgccaatc cgtgtaaaac cctaaatgac ctgtatgcac
    42841 gcccaagaaa cgcacatcat tcagacaatg actgtagtga tggaacgccg acaataatgt
    42901 gccgtctccg ccgactgaga tgaccaattc tggctcatga tcatctcgtt gaatccctgc
    42961 ttgttctaaa agtgcgttca aacgttctgt cacttctata gtctttgctt ctttattatg
    43021 aacgatcgct actttcatcc cctgcctcct ctctttcgct ttctttcttc aaaagcgttc
    43081 gatagtagac atcatctgat tttcctcttc catgggagaa taaccgctgt gcttcttgga
    43141 tttcttcacg gatactggac atctcttcat ccaactgata ggcagcttct gccgcacgct
    43201 gaagtcgttc actcatttct tctggaaaaa cgccttgata tttataattc agcgaatgct
    43261 caatcgttgc ccagaaattc atcgcaagag tccgaatctg gatttccgcc aaaattttct
    43321 tctcaccgct gatcagctgt acaggatact cgatcaccag atgataagaa cggtagccac
    43381 ttgctttttt atttgtgatg taatctcgtt cttggagaac tttcatgtct tttcttttac
    43441 ggatgatctc aacaacttga tggatatctt ctacaaactg acacatgatc cttaacccgg
    43501 caatatcaga catttcttct tctaaacgtc ccattggaat atgccgtaat tgtgattttg
    43561 ttacaatact atcgactggc ttgactctcc cagtgacaaa ttcaatcggg acatgtcgat
    43621 tttgttcacg gaactgtttg cggatccccc gcagtttcac tttcaattct gatacagttt
    43681 gttcatatgg tgctaaaaat gcttcccaat ctcgttccat agctgatacc tctacttcat
    43741 taacattcat tgtttattct accatatatg caaagttttt tcgtactctt gatttatgca
    43801 ttgaccaaaa aaagaaaatt aggcaaaata gacaaaaagg agtcgatcaa atgacacaaa
    43861 tgcttgaaat cgaatataaa tctatgctga caaagcaaga gtacgagata ttcatcgctc
    43921 actatcaatt aacagataaa gatttccgtg tacagactaa tatttatttc gataccgttg
    43981 atgaacagct gaagaaacaa aattgtggcc tacggatccg tttcgttggt catcaagctg
    44041 aatatacatt aaaaacacct gctgaaaaag ggcgtctcga aacaacggat acctttataa
    44101 cagaagaagc agaagcattt atcttcgaaa aacgtctgcc tcgttcaggc gcagtttttc
    44161 aaaaactttc tgatttgaat atcgatcctg cttcattaat ccaaaccgga aagcttacaa
    44221 caaaacgggc tgaattccct attgaagaag gacttttagc tattgatgaa agctggggag
    44281 acaatctaca cgattttgaa ctggaactag aggtaagggg atgcatctgt gggaaaaatt
    44341 gcttttataa attttttaaa acgtttttct ttaccttatc gtcctgctaa gaacaagatc
    44401 caacgtatgc ttgaagctaa aaattagaaa cctttcattt tcacaaggca cttgagttca
    44461 tttttttact tttttctgtt aagttatagg atgtgaaaga atctagtcac tagttgtata
    44521 gaatctattt tgggggagat tcatatgatc gaaatctatt tatttgttaa tcctttaggg
    44581 gaatactgtt tcgaaatgga acaacagtta ttacaattta tcgaagaaga gtacgggaca
    44641 ccatcaaaag aaaagatgca atttcgcttt cttcctttgg tcaatttgca gacaattggg
    44701 gatgtcatgc aacaaaaagg aatttctcag aacaatcttg aggaacgtaa tcacttattc
    44761 tcaatgactt attctgcagc attggactgc aaagcggcgc agtttcaagg aaagaaaaaa
    44821 ggccgccgtt ttttgatcaa attgcaagaa gcagtcggct gtaatgacat accatattct
    44881 caagaacttg ctgaatccat atttgaagaa atcggcggcg atttgaatat gtttaaagaa
    44941 gatcgtcagt ctgacttcgt taaagaagta ttcttttctg atcaaaaaat tgctcgggaa
    45001 atgggcatca caaaacaccc ttctgcagtc gtttacaatt atgcctgcga ccgtgatttt
    45061 ggtgttttag tagaagtaaa aggtacaagt agcctttgga gaatcacaga gctgtgtaaa
    45121 acaacagata actcatatca agtttttaat ggtgaacatt tacatacaaa tgattctcag
    45181 ttccattcaa gaactagcca tttacacttg ctttctaatt aaaaaaatac gtcgactccc
    45241 ttacaagaga gccgacgtat ttttttcgtt tctattcgag aaaatcaaaa atagaaagcc
    45301 ttataaacga tcgatcaatt cttctaattc atttagacgt tgttcaaaca tcgacattgc
    45361 atcttcaata tatgcagcct gtgtcatatc aactcctgct tttttcatta cttccacagg
    45421 ataatcgctg ttacctgctt ttaggtaagc caagtagttc tctagtgcct ctggttcctg
    45481 gtttagtatt ttcttcgcca gtgctgaagc agcagaaaag ccagtcgagt attggaaaac
    45541 atagtaatta taataaaaat gcggtatccg tgaccattcg aatttaattt ctgggtcttc
    45601 ttcgactgct ggaccataat attttgcatt caatttacca taactatcac ttaggtattc
    45661 actagtcaat ggcacacctt tttcatcttc cgtatgcatg aaatgctcga attcagcgaa
    45721 ttgtgtctgg cggaaaactg ttcctttaaa cccatccaag taatggttga gtacataagc
    45781 ccgtacacga gggtcttttt ctgtttccaa taaatactcc gttaggatat tttcattcgt
    45841 tgtcgaagca atttctgcta aaaagatgga gtagtcgccg tacacataag gctggttcga
    45901 acgagtgaaa taactatgaa cactatgtcc catttcgtgg acaagcgtaa atagctgatc
    45961 cagcgtatca tgccaattca ataaaatata tggatttgtg tcatagcttc ccgaagaata
    46021 agcaccgctt cgtttccctt tattttcgac aacatcgatc caacgttcag agaatgcttt
    46081 ttctacgatg gccatgtatt cttcacccat tggtttcagt gcttctaaag ctttttcttt
    46141 tgcttcttcg tacgtaaagg taattggagc ttcccctaag accggtgtat aaagatcata
    46201 catgtgcagt ttttccactt ctaataaacg tttccgtaat tccatgtatc gatgtaacaa
    46261 aggcaaatgc ttgtttacca cgtctaccaa agtatcgtat acactttcag gaatatgatt
    46321 attgctcaaa gacgcttctc tggcagagct gtaattacgg actttcgctt taaaattatg
    46381 tccttttata tgtgtgccta aagtagaagc aaatgtattt ctaaattgtt cgtaaacact
    46441 gtacaatccc ttaaatgctg cttcgcgcac ccttcgatcc gtgctttcta gcaactgacc
    46501 atacacgcca tgagataatt ggactatttc accattttct ccttcaatcg ttggaaaaac
    46561 tagatctgca ttattcaaaa cagcaaatgt atcacttgaa gcatcaaaga tttcacctgc
    46621 tccagcaaga agagattcct gctcagcaga taagacatgg gctcgattat ctactatttg
    46681 ctggatataa tggcgataga cttccaattt tggttcttct ttgaaatatt gccaaatctg
    46741 gtcatctgac aattgcaata tttctggttc aaaccaggaa acggcttcac taactttcga
    46801 aaacagactg cttgctcttg cataaagcgc ttggtaatct gtatttccag tgtcttgatc
    46861 gtttttaagg tgcgcatata cataaatgac ttcagtttgg cgataaaccc tcaatacgaa
    46921 ttcaatcgca tttaaaaatt gagaagcgcc ttgatcaagt gttcctttgt gtttttcaga
    46981 ttgttttaac tcttctgata attccaaata tttctcatca aactcttggt cgcttgaaaa
    47041 gatcttggtc aagtcccaag ttaaattttc aggcaattct tctcgttttg gcaactgctt
    47101 tacttccatg aaatccactc cttttcttcc cttagctcta tctatcaaaa tagatcatag
    47161 tctaacatcc acatgtcaca gcatctaact tttctactca ttcatgtgtc tgcttccatc
    47221 aattttagca taactaaatc ttgttttaca tagaaaaccg attcctagcc tatttttata
    47281 cttactattt tttcaaagaa gacaagtgta tgcattcgtt aaaaaactgc tgatagacca
    47341 tctcttcatc aatcatagga aaaagccatt cccttttatg ttcttttatt tggcaaagcc
    47401 atgagttgaa atcaggaact ttctggtttt ccaaggcttc ttcgtacaac attcgataaa
    47461 gaaaaacctg ttcttgtaaa taaaagaaaa accgactttt ttgatagatc cacgatgata
    47521 gatgcaaaag atgtttatga cgcaagtagc attgttcttg tatggatact gtattctttt
    47581 gcttgtgaat aagttggcgt gtcagccatt tcttcccatc ttcactcact ttttctattt
    47641 ttttcatttg gttcatttta atttcgttac tagtgaatat ttctttaagg tccctactag
    47701 aaaaagtcca gcagctactg caaaaggaaa tatgccctga tacagtctct gtaatatgat
    47761 gatacaaata aagcttttgt ttcttgagat cagctttcca gcaatgcact cctctttttc
    47821 tattatagta gcagcaactt ttttctatca atgagaattg cgattgtcca agaaaagaat
    47881 taccaagtat ccaccatact tggtaactgt tattcaaata attttgggtt ctttcaataa
    47941 atctctggtg agacagtcta gaacattgga tttctattgc tagattcgcc aacaaaatat
    48001 ccggcctttg ccgcaacctc ggcaaatagg cttccaaaaa tactggttct actgactgtc
    48061 ccagccaatc catgaagcat tcctttaact gtaagtgctc ttttgattct ctttcactaa
    48121 agaccgaaca gttatctgat ttttgatggg caaaatgagc acattttttc tttccctttt
    48181 tcaaaatcac tctttttcga cagccacgac aataatattt ctttcctttt tctgcatgtt
    48241 tagcaaaaca gcacttatta tattcattca ttgcaagcag catttttctc cctctgaact
    48301 tatttacgtc taagaattta tttacacgaa aaaagagggc gtgaaaaagc tattctgcat
    48361 caagtaataa gaacagctaa acaaaaatag ttcccaatat ttttcgtttg gttgggcttg
    48421 ttaccgcaga tgcaagcttt tgaacacatt tatacgaaaa aaagactgcg acacaatttt
    48481 tgtcacagtc tcttactata atgctgattt atacaaaata aaatttcgtt cgctcgatag
    48541 cattacgcgc catcagacat tgtccatatt cattcaatgt ttctggtgta cgatcgctct
    48601 tttcagcaaa ctccaaaatt cgagcaatct gattctctac atccgcttgg ttgagatttt
    48661 ccatccagaa gtgaacgact aggtaatagc gctcttcgaa taaatacaaa tcagtccaag
    48721 cagactctag ttcgacatct gttgcaagtt ggatcacttg ttcaaaatct tcaaaagcaa
    48781 aagttgcttg tctttcctgt tcttctaggg cgcttccaga taattcttcc tccggatcgc
    48841 tgccaataat ctgctgacga agaaagtcac tcacatcttc tgaatcaaca gtagaatctt
    48901 ctgattgaga aatttcttca tccggaagat ttttactgat aaacagttct aatccgtctc
    48961 cttttggcaa aacttggaaa gtgacagctt cgcttcctcg gaactcctct tcgatatcaa
    49021 cttcttccaa aatactataa aagaaatttt cgatttcttt gtgattgcca agtaaatcca
    49081 aaaatgaaac gccccgttcc tctaaatctt catgaccaat tagtacacgg atcgtatttt
    49141 cattaatatg ttccatttcc attttgctac acctcacttt gccacaatgt ttaatataga
    49201 ttcattgtag cgaaatcagc ttaaatgtaa agaaaaaaaa tctcgctgtt aagcgaattt
    49261 aataaaacca gcggattttt caacctttca agctatctta tttaactata acatttttgg
    49321 cagacgaata ctatatagcc gacttttcag ctaattttcc ctctctattt ttcgtgcaat
    49381 catcatcttc tactaccttt ttatacatct aatcaagaat acttt
//
LOCUS       DAZKLA010000005        46678 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000005 DAZKLA010000000
VERSION     DAZKLA010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.005"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            164..1045
                     /locus_tag="ACG85N_000212"
     CDS             164..1045
                     /locus_tag="ACG85N_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0223015.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            1058..2668
                     /locus_tag="ACG85N_000213"
     CDS             1058..2668
                     /locus_tag="ACG85N_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0223016.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2745..3404)
                     /locus_tag="ACG85N_000214"
     CDS             complement(2745..3404)
                     /locus_tag="ACG85N_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0223017.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3687..4352)
                     /locus_tag="ACG85N_000215"
     CDS             complement(3687..4352)
                     /locus_tag="ACG85N_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFQ0223018.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4499..5104)
                     /gene="dhaL"
                     /locus_tag="ACG85N_000216"
     CDS             complement(4499..5104)
                     /gene="dhaL"
                     /locus_tag="ACG85N_000216"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFQ0223019.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5132..6142)
                     /gene="dhaK"
                     /locus_tag="ACG85N_000217"
     CDS             complement(5132..6142)
                     /gene="dhaK"
                     /locus_tag="ACG85N_000217"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFQ0223020.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(6117..6512)
                     /gene="dhaM"
                     /locus_tag="ACG85N_000218"
     CDS             complement(6117..6512)
                     /gene="dhaM"
                     /locus_tag="ACG85N_000218"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFQ0223021.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6666..6986
                     /locus_tag="ACG85N_000219"
     CDS             6666..6986
                     /locus_tag="ACG85N_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFQ0223022.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(7098..7955)
                     /locus_tag="ACG85N_000220"
     CDS             complement(7098..7955)
                     /locus_tag="ACG85N_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0223023.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(8063..9367)
                     /locus_tag="ACG85N_000221"
     CDS             complement(8063..9367)
                     /locus_tag="ACG85N_000221"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0223024.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9364..9873)
                     /locus_tag="ACG85N_000222"
     CDS             complement(9364..9873)
                     /locus_tag="ACG85N_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223025.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(10048..12183)
                     /locus_tag="ACG85N_000223"
     CDS             complement(10048..12183)
                     /locus_tag="ACG85N_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFQ0223026.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12266..13921)
                     /locus_tag="ACG85N_000224"
     CDS             complement(12266..13921)
                     /locus_tag="ACG85N_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0223027.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13923..14237)
                     /locus_tag="ACG85N_000225"
     CDS             complement(13923..14237)
                     /locus_tag="ACG85N_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0223028.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14407..14667
                     /locus_tag="ACG85N_000226"
     CDS             14407..14667
                     /locus_tag="ACG85N_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFQ0223029.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14681..14995
                     /locus_tag="ACG85N_000227"
     CDS             14681..14995
                     /locus_tag="ACG85N_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFQ0223030.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15725..16279)
                     /locus_tag="ACG85N_000228"
     CDS             complement(15725..16279)
                     /locus_tag="ACG85N_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFQ0223031.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16405..18334)
                     /locus_tag="ACG85N_000229"
                     /pseudo
     CDS             complement(16405..18334)
                     /locus_tag="ACG85N_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18640..19356)
                     /locus_tag="ACG85N_000230"
     CDS             complement(18640..19356)
                     /locus_tag="ACG85N_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0223032.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19357..21180)
                     /gene="glpO"
                     /locus_tag="ACG85N_000231"
     CDS             complement(19357..21180)
                     /gene="glpO"
                     /locus_tag="ACG85N_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFQ0223033.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21194..22690)
                     /gene="glpK"
                     /locus_tag="ACG85N_000232"
     CDS             complement(21194..22690)
                     /gene="glpK"
                     /locus_tag="ACG85N_000232"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFQ0223034.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22826..24286)
                     /locus_tag="ACG85N_000233"
     CDS             complement(22826..24286)
                     /locus_tag="ACG85N_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0223035.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24276..25571)
                     /locus_tag="ACG85N_000234"
     CDS             complement(24276..25571)
                     /locus_tag="ACG85N_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0223036.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25903..26580)
                     /locus_tag="ACG85N_000235"
     CDS             complement(25903..26580)
                     /locus_tag="ACG85N_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFQ0223037.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26610..27701)
                     /locus_tag="ACG85N_000236"
     CDS             complement(26610..27701)
                     /locus_tag="ACG85N_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFQ0223038.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(27802..28236)
                     /locus_tag="ACG85N_000237"
     CDS             complement(27802..28236)
                     /locus_tag="ACG85N_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0223039.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28820..30277)
                     /locus_tag="ACG85N_000238"
     CDS             complement(28820..30277)
                     /locus_tag="ACG85N_000238"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0223040.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30274..32169)
                     /locus_tag="ACG85N_000239"
     CDS             complement(30274..32169)
                     /locus_tag="ACG85N_000239"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0223041.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32227..34002)
                     /locus_tag="ACG85N_000240"
     CDS             complement(32227..34002)
                     /locus_tag="ACG85N_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0223042.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(34052..35548)
                     /locus_tag="ACG85N_000241"
     CDS             complement(34052..35548)
                     /locus_tag="ACG85N_000241"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0223043.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35742..37364)
                     /locus_tag="ACG85N_000242"
     CDS             complement(35742..37364)
                     /locus_tag="ACG85N_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0223044.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37692..39005)
                     /locus_tag="ACG85N_000243"
     CDS             complement(37692..39005)
                     /locus_tag="ACG85N_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0223045.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39250..39474)
                     /locus_tag="ACG85N_000244"
     CDS             complement(39250..39474)
                     /locus_tag="ACG85N_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223046.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39494..39916)
                     /locus_tag="ACG85N_000245"
     CDS             complement(39494..39916)
                     /locus_tag="ACG85N_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0223047.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(40058..40768)
                     /locus_tag="ACG85N_000246"
     CDS             complement(40058..40768)
                     /locus_tag="ACG85N_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFQ0223048.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40786..41283)
                     /locus_tag="ACG85N_000247"
     CDS             complement(40786..41283)
                     /locus_tag="ACG85N_000247"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0223049.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41320..41772)
                     /locus_tag="ACG85N_000248"
     CDS             complement(41320..41772)
                     /locus_tag="ACG85N_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0223050.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41907..42512)
                     /locus_tag="ACG85N_000249"
     CDS             complement(41907..42512)
                     /locus_tag="ACG85N_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0223051.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42962..43582)
                     /locus_tag="ACG85N_000250"
     CDS             complement(42962..43582)
                     /locus_tag="ACG85N_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0223052.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43584..44453)
                     /locus_tag="ACG85N_000251"
     CDS             complement(43584..44453)
                     /locus_tag="ACG85N_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0223053.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44476..44883)
                     /locus_tag="ACG85N_000252"
     CDS             complement(44476..44883)
                     /locus_tag="ACG85N_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0223054.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44902..46191)
                     /locus_tag="ACG85N_000253"
     CDS             complement(44902..46191)
                     /locus_tag="ACG85N_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0223055.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            46249..>46678
                     /locus_tag="ACG85N_000254"
     CDS             46249..>46678
                     /locus_tag="ACG85N_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0223056.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT        13167 a         9913 c         7998 g        15600 t
ORIGIN      
        1 caagctagca attccttgtt tatgaattca ttgacagaaa tttataaaaa gaatattatg
       61 tactaaaata ataaaaaaag ttttataaaa aggtataaaa cctttttatt aaactattta
      121 atcttgttta gtatatattt ttagaaaaag gagtggggtt tttatggcag aaatcgtcaa
      181 agtcacacat ttgaaaaaga gctttggtcg tttcgaagct ttaaaagatg tctctttcac
      241 cgtacattca ggtgaagtcg ttggatttat cggaccaaat ggttctggaa aatccacgac
      301 gattcggaca cttttaggga ttataaaaaa agacggcgga acggcaacga tttttgggca
      361 agatgtatgg gaagatagtt tatctatcca tgaacgtctt tcttatgttc caggcgatat
      421 ctctttgtgg ggaaatttga ctggaggagc gatcatcgat ctcttcatga agcttcatgg
      481 aaaagggagt gctcaaaaaa aagatgaact gattcaacgt ttctctcttg atcctaagaa
      541 aaaagcaaaa aactattcaa aaggaaatcg ccaaaaagtg ggcttgattg ctgctttggc
      601 tgttgattcg gatctctatt tatttgatga gcctacttct ggtttagatc ctttgatgga
      661 aacgattttc caagaagaag ttgaaaaatt gaaagaacag ggcaaatcca tcctactttc
      721 ttctcatatt ctcagtgaag tcgagcgact agcggataaa gtcgtgatca tacgcgaagg
      781 aacactcgat gaagaaggaa cactcgatga attgcgtcat ttgactcgtt cgaccatcac
      841 tatagaaaca gaagcagaga atagtcactt agcacaattg aacggtgtcc atgactttgt
      901 gcaaaaaggt cacctcgctc aatttgctgc tgataacgaa gctttcgatg tgattttagc
      961 tgaagcgtcc aaattaggtg tgaaaaaatt tgaatctatt ccaccgactc ttgaggattt
     1021 attcatgaga cattatgaag gatgaggagg aagcgaaatg actgataaat tttcacgctg
     1081 gtccaccctt ttttctcaaa accttagccg tgactggaaa aagatcctga tatggctagt
     1141 cggtgtcact gctttttctg gtggttttgt ccctgcgttt gaagaattga cttccggaca
     1201 aggattagct ggattatacg aaaccttgca aaacccggca atgattgcaa tggtcggacc
     1261 aactccagct tcgaatgctg ctgagtatac tttaggagcg atgtatgcaa atgaaatgtt
     1321 gcttttttgc agtttgtttg ctgctgtgat ctctgtttta cacgtggtga gccaaacgag
     1381 aaaaaaagaa gaacttggtc tgattgaact tattcgttcc tttcgagtcg gacgtcaagc
     1441 aaactcttta gctgtcatcg ctgaaagtat tctgatcaat ttgcttttag caatatcgat
     1501 cagctgtacg atgatttttt tccaagcgga ttcgatcact gcagaaggtg cttggctttt
     1561 tgggctatct gtaggtagta tggggatttt ggggagtgcc gttgcactgt ttgctgcaca
     1621 actgatgcca agttcagcca gttcgaatgg tctgtcttta gcgtttttag gtggacttta
     1681 ccttctacga gcaataacag atgtagctga ttcctccctt tcgatgtgga atcctttagg
     1741 ctggtcttat ttgacctatc catttacaac taacaactgg attcctttgc tttatttgtt
     1801 tttgttcagt ctaatcttga taatcagttc atttgtttta gaggaacatc gagatttgaa
     1861 caccggctat atccccgaac gtgaaggaaa accgtatgct aaacgttctc ttttatcctt
     1921 gcgtggttta ttattctatc taaataaagg gatgacaatc agttggctta tcacttttgg
     1981 tttgatgggg gcggcatacg gctctatcta cgggaatatg caacagtttt tagaaagtaa
     2041 cgacttaatg aaacagatgt ttatccaatc tggcgtctca attgaaaaaa actttacaag
     2101 cacgatcatg gtagtcttga tttgtctagc tgctatttta ccaattgcta gcatcaatcg
     2161 tttatttaca gaagaaaccc acggacgtat gaatcagctg ttcgcaacaa aaatttctag
     2221 gaaccaactt tattggacca atatgttact agctattcta tttgctatat tggcaatcct
     2281 gattagttct ggtgcactag ggctttctgc tttggcttcg atgaaggatg cgaaagacat
     2341 ggatctattg atgtttctta aagccgggtt caatttcctg cctgccgttt tagtgtttat
     2401 cggaattagt ggctttctat tcggatggct tcctcgaatc ggtaaacttg cttatatcta
     2461 cttagcttac tcctttgcac tcaattactt tggcggaatt ttggatttac cagaatggtt
     2521 ttccaaaaca gctgtcccta gctgggttcc acggatgcca aacgattctt ttgatctttc
     2581 cgtgttcctt acgttatcat gtatcagcct tgcactgttc gtccttggtt ctattggtta
     2641 taggcgaaga gacttactag aaggataata aaaaagcatg tacaatttca tgctagcctc
     2701 caaatctggg tcaaaaatcc aacatttgga ggtcattttt ttatttacaa gtcttctaac
     2761 tgctctttat caatgatcca gacttctcga ttctttcttt ttacataacc attctcctct
     2821 aataatttaa attttctaga taaagtttcg ggggtcgtac cgataaatgc agcaagttct
     2881 ttcattttca ttggcaggaa aaagtgatcc ttccctgtcg ctttagctaa gtcaagcaga
     2941 tagacagcga ctcgttcttc tactttttcc atcatcaaaa aagttgtctg ctgctgtact
     3001 tttgttaatt tctcaacgtt gattgctaaa agtttcagac taagggcggg ataatgcagt
     3061 aagagttccg agaaatcttt ttgtctcaat atgcaaattt ccgttttttc tagtgcttcg
     3121 ccaaataaag actcatttgg aacgcctagc aactggctct ctccctcata tccgcccggc
     3181 tctattactc gtaaaagctg ttcctttccg ctagcagata attgatacac tttcatactg
     3241 ccggaggcca cgatcactaa ttgaggttct ccattcggcg caagaatctg ttctcctttt
     3301 tccacatagt gatgtttgac aagtgcgtgt actttttctt gatcttgctg tactaagtga
     3361 ttgaacaaag gaacaaatga aacacataaa tgatcatgac tcataatctc tcctccttct
     3421 aaacagctat ttctaataaa gaaacataac ttcccttatt ccaactgata attttctttt
     3481 atcatagcat aaacgtcaag cgaaccagat accagcaaat agagtggatt tctttttgtc
     3541 ccttgcttta ttttccttct tttgagacct taagtaattg attcctatca attttttaaa
     3601 tatttacttt ttgaataatc aaaaacaaac cttcactcgc aagccgagag taaggtttgt
     3661 ttttcgcatt gcttatttcg tgttgtctat tgaaattttt ccgcacctag cttttgatat
     3721 agtggtgcta gttcttgtaa ggtttcagcg gttatttgct caacgtgttc gatttcttgc
     3781 ggatagaaga atagtttacc catttgaaag gttcggtctc tcatcgcttt tttcaactct
     3841 tcttgtttct ttgtatcgga caaatagtca gacaaagcta agtggtcgtc aagttcatgt
     3901 tctggttgcg gccatatgta ataatccttg atatcgactg ttttgctcca ctctttcagt
     3961 atcgcaagca actggttata cgctgcaatg ctaccatatc gttcttcgct tttataatgt
     4021 tggtacatca aatagatttg gtattgtttt tgattcaaca aaactgctag acaggcattc
     4081 tcgtcttgac gagattcgct tcggtacgcg ctccaaaaat gactgcgtaa attccagcca
     4141 ttcgtccagc tttcgatttt aggtttgctc atgccgtaca tatcaggcaa aagtgcagct
     4201 gtctggagct gaatctcttt ccatttttgc cagtgttctt tatattcttt cttgatttgt
     4261 tcaatcttat ctgttggatg gctctctttg agctgtttga atgcgtagat tggtcgatca
     4321 aacaactgat aatctgattt tccaagttcc attgtttcgc ctctttccgt tatcttttgt
     4381 attctacctg aattttgcga tatggacaaa tagaggttgt gaaaaagctg ttctgcatca
     4441 agtcataaga acagccaaac aaaaaactgc gatgagcatc accgcagttt tattgaactt
     4501 aaggttgatt gatcacatct aaaagcgttg aaaataaaat ggcactagaa gcagctcctg
     4561 ggtcgatatg tccgattgca cgctctccta gataggaagc tcgtcctttt ttagctacca
     4621 agactttggt gtgttctttt gcctggtcaa tgatttcttt tgttaaattc cctgccttca
     4681 atgcctctgt gactggcagc caaacatcta acatcgtttt atcgtcagct tctgcctttc
     4741 ctcggctttg aatacccgct gttccctgct cgatcacttc gcctaatttt tcttgagaat
     4801 caatagaatc gacctctttt gtcgctttca tcatgttcat gaatgcagat ccgtataacg
     4861 gaccagaagc cccaccgact ttcgatatca tcgccataga gaatactttg aatgtatcgc
     4921 tgattgtttc aggatgatcg gcttgaaaag cttcttcata agctgaaacc cctcttccca
     4981 tgttatttcc atggtctcca tcgccaatcg gggtatctag atcactgaga tattgtttgt
     5041 tctgattgat tgcctctgca aattgcgtga gccattcttg gatttcttga acggttagtt
     5101 tcatattatc acccttttat cttgagattt tttaccatga aatcgtctgt acattgcttt
     5161 ctaaagaatc tttccattgt gtctccgtca agtcgatcag tgttagagaa agaccttgca
     5221 tatctagtga ggtcataaaa ttaccgactt ttcggaaaac tacattgacg cctctttctt
     5281 ccaacagagc taaaacatca ttcatgaaca cgaactgttc catcaaaggc gtcccgccca
     5341 ttccgttgac tagtacacct gcttcttttg gtaaattcga gtaatcatct aaaatcttag
     5401 taacaagttc ctttgccagt actttagaag gctgcatctt ttctcgacga tagcctggct
     5461 caccgtggat accaacacca tactcgatct catcttctcc tagttcgaat cctggtttcc
     5521 ctacttctgg tacagttgct gctcgaagcg ctagaccgat cgtcttagtt gcctgtacga
     5581 ccttttctcc cagttctttc aactcagcta gggaagcacc gtttcttgct gcatcgccca
     5641 cgattttgtg tacaaggaca gtacctgcaa ctccgcgttt tccagcagta tacgtactat
     5701 tttcaacggc gatatcatca tctacgatca cactttctac ttcaatatcg tccatagcag
     5761 ctaattcctt tgccatatcg aagttcaatg catcccctgt ataattctta acgatcagca
     5821 aaactccttt tccactatcg gcttccttaa tagccgtctg aatctgatct ggagttggag
     5881 aagtaaacac atcaccaagc accgctgcac ttaacatccc ttcccctaca aacccggcat
     5941 gggatggttc atgtccgctt cctccaccag aaaccaaacc aacttgttcc gctttctgat
     6001 tcttggctac aacacgggaa gattctacac gatgaacaag ttctggatag ctttttacta
     6061 atccttccag catttcttcg acaatattgc ttggatcatt catgattttt ttcaatttat
     6121 tcccatcctt tctttccggc attttgtgct tctgttaaga tttgctgagt atcatctgtt
     6181 ataccggcag tgatggctgc tgcaaaagct ccttccacta aaggcgcatc caccagatga
     6241 taacgttgct gctgctcttc ttccaacatg tcttttgcca gttccgagtt caataccgcg
     6301 ctaccgatat ccgcaaaaac aaaataacta gctgcatcgc ttgattcatt gactgcttct
     6361 acgattttca tcgggtctga tccgatctcg ccatttattc cgccgagaga aaaaatttgc
     6421 acctcttcgg atttttgcat ctgctcaatc atttctttaa taccatctgt tactttttga
     6481 ctatgagaaa taagcaaaat actttttttc acaagaatct tcctttcttt tacatagttg
     6541 ctaacgattg gtttcgcttt cattaatgaa caattcaagt atataataaa tttctaattt
     6601 tgggaaaata tgtgtagtgc ttcttgaagt ttgttatact ttaattaaaa gagaggagtg
     6661 atcgaatgat tacaacaaca acgaacagtg tagagggaaa agccgtagta gcttataaag
     6721 ggatcgtttt tggtgaggtc attactggga tccatgcatt taaagacttg gaggcggggt
     6781 tgcgaaatat ctttggcggc cgctcaaaaa gctatgaaaa cgaactaatg ggtgcaagag
     6841 aagaagcact tgccgaaatg gccgaacgtg caaaagcatt aggagctgat gcgatcattg
     6901 gcgtaaaaat ggactatgaa gtattaggca gcgacaatgg catgctgatg gtcacatgta
     6961 gtggaacagc tgtctccacg agataagtag gggacatctg ttttaataac taaagagaga
     7021 tgggacatgt agtggcactc aagaattaga tttttcagtc taacttcgag gtactctacc
     7081 ttcccatcct ctctttttta tttctttttg cggatgattc cgtaagctgc tgcgactaac
     7141 acacccgccc ctgctaagga ataactgaca gaagctgttt tttcatttgt ttttggcaaa
     7201 gtctgacttg ttgatttgga tggctctgtc gtttgctgaa ctgtttctgt cgtgcttcca
     7261 gaggtcactg atttttctcc tgccgaatgc aaaatatctt ttattcgttg gaaactagct
     7321 gctaagtcag ctttttttgc ttcaaaatca ttagcaactg tttgtgcttc gctgatcttt
     7381 ccgtggattg cttgactggc cgctgtgata tccgcttgag tttgacttag ctttccagaa
     7441 gctgtttgag caacctgatt taattgctga ttgaattctt ttgcttgttg gatcttcccg
     7501 tcaagatctg tttggacagc ttccgctttt gctttcgctt cggcaagctt tttctcaagt
     7561 tgtgcacggt catttcccac tttattttta agatcagcta aatcttgatc gattgctgct
     7621 tggtcttctt tcaatttgtt ttttgcagct tctaatgctt cttcgagttc ttttctttta
     7681 ttttccaagt cttgcaaatg atcgatttgg tcagaagagg tagaactatt tattgatgta
     7741 tcttctgaag atgaaatcgt ttctcctgaa ttatctgtcg gtacattgga agtcatttct
     7801 gtttccgagg caccgttttc agcaccagaa gagcttatgg ttgttgaagg ttctgtttga
     7861 gcaggttcag aagtttccgc aaaagctgtc tgtcccaata agaaaatact ggaaacagca
     7921 attggccaga ttaacggctt cattcttttt ttcatcactt ttcccatcct tactactttt
     7981 taactaattt cttattataa gcaaaaatgt ttggaagaac cataaacaca acaggctgtt
     8041 tgtctaaaaa acggtgcatc ttttattttt ttgtacgttc cgggcttttc catgtcccgc
     8101 ttccataacc taaaatcgtt ttgactgctg gaatgaaagt cgtcacgatc gttatcgcgt
     8161 tcactatcca gaacaaaagc atatatagtg gtgcgaaaag aaggtatttc aattttcttc
     8221 gtcgatcatc tacaatcagc gaagctagca actggaaaaa tcctgcgacc atttcaaagc
     8281 agatgaacac aaaactgaat gtaaaagtaa tataaatctg ttcgtagttt ccttgatata
     8341 cataatgccc gattaatcca gcaaaaagaa ctacggaaat acaaaagaat attgaccaga
     8401 caatactcaa tgtttgatca atgaagatga tcgttcttcc aatatgttta aaaggatgga
     8461 gcatcacttt tttgaaattc gtcagccaga cttctgttcc accttttgcc caacgttttc
     8521 tgtgtcgata tagcatcttc aacgtcacag gtacttccat gaaaaagata atctcaggag
     8581 caaaaacaga taaccagtca tttaactgat gatcccaagc aatactgatg tcttctgtcg
     8641 ctcgatcttg tctgaatccg cccacatcga tcaaggcttc ttttcgatac atcgtatttg
     8701 ctccactgta cgcataaaga ccaccgaaaa cagctgactg tgttcgttta atgatgccca
     8761 cgatacttga gaattcaacc gtttgagatt ttgcaatcaa tttggtacga ttttggacat
     8821 ccatatttgc agtgaccgca gcaatatgtc ttgcaccagg gcgaatgaaa taattcacat
     8881 acctaatcag tgcatctggc tccggtaccg tgtctgcatc attgcttaaa atcaatttcc
     8941 cttttgcaaa ggcaagtccg atattaaacg cgtgggcttt tcctttattt ttttcgatcc
     9001 gaaccacacg aagattagcg tattttttca tcagtctagc taatatttct ggtgtttggt
     9061 ccgttgaacc atcgtctgtc actaatacct catagttgtg ataattcaat ttcgtcatca
     9121 aatactcgat tgtatattcg atcacaattt cttcattatg ggctggcacc atgatcgtga
     9181 tgaacggttc gacactcaag ggaacatcta cccattcttt ttgcttatgt ttgtataaaa
     9241 acacataaca ccagacacca ataaaccata caaatccgcc taaaatcgga taagaaacga
     9301 gaaaaaaata cataatagac aaacaagtat ctaagaataa attacctgta tgggtgattg
     9361 agatcatttc agttccaccc cattttcttt atacaaatct gcaatgaatg tcgtatctag
     9421 atttttctct tcggagacag aataaaaacg gaccgtttct ctctcttctt ttgttccaaa
     9481 acgttccgta tacacttctt caagtaattc ttgtcttttt ttcaattttt catcttcata
     9541 ttcaaaagat ttatttactt ttttgtatag atttcgattg ttccacctag tcaatacaaa
     9601 tgaggtaatc aggaaactga aaaaaatcaa tacaaaaaag ccgattaaga aatacagcat
     9661 tttcttttct gataaaaaag cgtacaagga aaaatccttg gctctttctg gtctcgttaa
     9721 tgggaaaaag acccatataa aaggaagaac aacgatcatc catcctaaaa gagccactaa
     9781 cgtttgccaa atctttaacc agtaattacc ttttgaaaaa tacttatctt tcaccaaaat
     9841 tgatgcaact tcttctcttg ttactttttt catatcctca cctcacgttt ttttgttacg
     9901 acgtaggagt ttaccataca tttcatttct tttcacataa agtgttccta aatttttcct
     9961 aagaaaaaga acaaaacatt tctcatcagc aaatgaaaaa tattttgttc tttttatcag
    10021 ttgttctttt tattttttat ctcttaatta tgttgctctt tccaatacga caatgctttt
    10081 cattcctcta gatatcgccg tgtacatttg tctgcgtctt ctttgctcat ctccatcgtt
    10141 ttcaaatgga tataagatta catgatcaaa ttccagtcct tttgctagac tcaaaggaat
    10201 gatttggcag ttttctaatc catatgaatt ttttttaatc gattcttgta gcgtttgggt
    10261 ttcctcttct gtttgggtga taactgccgc tttttctcct tttagagaaa ccaaaatctc
    10321 agctagtatt ttcaacaatt cttcagagga tcgaaaagag cggtattcgg gttcttttcc
    10381 ttgcggtcgg atcgatacga tgtcgatctt ctcttgatcc acagcatagg ttttgaaaag
    10441 ctctgtgatt gctccgctag aacggtaact atttctaaga tcataccgat ggatcggcag
    10501 atggtagtcg tcgaaacaac gcataatatc agcaaaagta gtggaagaat taaagattgc
    10561 ctgattctcg tcacctacta aagtaaagtg agtttttgga aataattcga tcaatagacc
    10621 taattgtgcc agggtataat cttgtacttc atcaatcaat agatagcgaa atgctggtac
    10681 atttaccttt tcaacaagta aatgacgaat agctaatata atgactgctt catcaacagt
    10741 acgtgtgggt tgccaagcat aagctgagcc atatcgtttt tcataaattc tttcaaacaa
    10801 ctgatgctca tccacccatg ccatttcttc gatttgtctg gttatttttc gatatttttt
    10861 ttgtaaaagt ttccttgcat aagctacaat actttgttcc gaatcatcag aaatcaattt
    10921 accgaataat tccaactgcc tatcttctgt caacgagagt acctgattct gcacagcagg
    10981 actttttgct tgtttcaaaa gatgattctt ccattcgctc atcagtgcct ttttcgttgc
    11041 ttgaagccta tcgatcaatt taggatgagc aggaactttc tcatatattt tttcgatcgt
    11101 ttttttagaa aggactgttt ttccattttt agtaagtcca cggataaaat tcggatgatc
    11161 taaaaacaaa ggatctgatt gttttagatt atcgataaac tttttgctac gcaaacgttc
    11221 tgtctgctcc gatacatttt ctcccgtgat acgtttgaaa taagcctctt caccttctat
    11281 ttcctctgct gaaagctgtg aaaccagctg tatgatggtt agattcagtg gatttctttc
    11341 acctaatgat ggtaggacat tcgaaatata gtcgataaac cgatgattag gtgatagaat
    11401 cagcaagtca tcggatgtca tcgtatctcg atataaatag agcagatacg caatacgctg
    11461 catgatagca gaggttttac cacttcctgc tacgccattt accaagatat tttgactttt
    11521 cacatcccga atgattgtgt tttgttccga ttggatcgta gcagtgatag ccttcatttc
    11581 attggtctca tcttggccta agacttctaa taatacgtca tcttgaatag ctacagacgt
    11641 atcaaaaaac ttgaccaaac gatctctttc caaaagaaat tgtcgtcttt ctttaataac
    11701 cgtatcgatt ttattttgac gaaccgtata agaagatgga ccaatcatgt tattgtaaaa
    11761 aagttcggca attggtgaac gccaatcata aacaagattt tcatctgcat catctgtgaa
    11821 atttgctgta ccgatataaa aggattcttt tggctcatta tccagaaagt ctacaacgac
    11881 ttttccaaag tagggacttt tcaataaacg ttcgatcttt gctagctgat tttctgcgtt
    11941 tttctgtttg atattcatct gatcgatttc tctatttttc atttcaatca tcgaaaaagt
    12001 atccaggtta tctaagtaac tgtcaaaatt caatcgaagc tcgccagcca tttctttcaa
    12061 cgactggttt ccttctattt ttgccgtatc catcgattcc cgcagttcgt tttttttatc
    12121 aattagcttt tgatacacgt tcttcagata tgcttcttct aactgttttt gtgtgttgac
    12181 catacacatt ctcctctttt tttaaagtgg tggaaccgat tagtttacct cttttaagag
    12241 tcgaatgcaa gctttttaac gagtatcatt tgttaggata tatcagttct tcaggtcgga
    12301 cagtttgata taaagaaaat gcaccatcaa ggttttttac gtggtagcca gactgcttca
    12361 agattctttc cgctagatag ctacgtaatc cgctatgaca gctgacgata tattcttgtt
    12421 ggctgtctaa ttcatttaat cggtcccgaa gttcattcaa agggatatgt ttggcttcct
    12481 tgaatttgcc cttttccaat tcttctgctg ttcgcacatc taataaaatt tttccttttg
    12541 ctagttctgt cggcaattca tgccattgta cggtttcact aagcccttct gcaatgttca
    12601 ttgctgcata tcctagcatg tttactggat ctttggctga gccaaatggc ggtgcatacg
    12661 tcaattctaa ttccggtaaa tcgaagatcg tcaaatgccc tttgattgct gttgctaaaa
    12721 tatcgatccg tttatccaca ccttttgccc caacaccttg tgcaccataa atctcgccag
    12781 tctctggatg gaagatcaac ttcagtaaaa tatctgttgc ttctggataa tagcttgcat
    12841 gatcttttcc tgatacatga atgacttcaa aagacagttc gttttctttg gctgttcttt
    12901 cacttaaacc ggtggatgcg gcagtcattc caaacgttcg tataattgct gttccaatac
    12961 cgccttgatt tcttcgagaa attccggaaa tcgtgtccgc aacttgtcta ccttgacgat
    13021 tggctggtga agcaagagaa ataagtgcat cttttccagt gatttgctgt tttacaacga
    13081 tagcatcccc caccgcataa atatcaggaa tattcgtttg ataacgttcg tctactagga
    13141 tacccccgcg tatgcctaat tcgattccag cggtcactgc tagctggtta tcaggacgaa
    13201 caccaatgga taaaataact aaatcagcag ggatggtacg cccatcatcc agtcgcaatc
    13261 gtttcccgtg gtcttcaaat ccagcgacag catgagaagt aatcacttct actccttgat
    13321 gagacaattc tttttctata aaagctgcca tttcttcatc cagtgttgga agaatatgtg
    13381 gtgcttgttc gatcaccatg acttctaaac ctcttctttt taggttttct gccatttcca
    13441 atccgatgaa tcctgcacca acgatcacgg cacgctttgg ctgtttttct aatgcatgca
    13501 tcactttatc gatatctggt acatttctaa tagaaaaaac attgtcggct tctgttatcc
    13561 ctggaatcga tggaacaact ggttttgcac caggagataa aaccaatgca tcgtactgtt
    13621 caatgtgttt ttgaccctca tgtacgactt ctaccgtttt gttttcggga aagatggctg
    13681 tcacttcatg atttggacgc acatctaaac aaaaacgagc ggataatgat tctggcgttt
    13741 ggacaagaag attttcccgt tcactgattt ctcccgaaag ataataaggc aagccgcaat
    13801 ttgcaaaaga aacatacggt cctttttcaa aaacaacgat ttctgcatct tccattaatc
    13861 ggcgaagccg tgtagcagca gacattccac ctgctacacc tccgacaata acgattttca
    13921 tcttatttcc ctcctactgt tcgatcaaac cattgattca tgccaccacg tacattcact
    13981 acatcataac cagatttctt taattcttta gctgcttgtt tactcctcat ccctgattga
    14041 caaatcacat agacggtctt ttctttgtct ccatcatatc tgtttatact ttgtaatgga
    14101 acatttttag ctccttttat atgcccgccc cggtattctg caggtgttct aacatcaagt
    14161 aacgctggat tttccaaaag cttttccttt aattctggga ctgttatcga ttttattgat
    14221 tgaaatagca aattcatttt ctgcacctca tttacatttt tataaatacc tcatggggta
    14281 tattaaacag gagctttatt aatatgtcaa ccagaatgac tcttcatttt tttcactact
    14341 ttttatattt gcataaaata ccgataaggg gtattatcta attataaaaa tagaggagaa
    14401 tgtgttatgg agattgataa taaaaatatc ctgaaccgtt tgaaacgtac agagggacaa
    14461 attcgtggta tccaaaaaat gatcgaagac gaaaaagaat gtatggatgt catcactcaa
    14521 ttaagcgctg ttcgttctag tattgatcgc gtaatgggca tgatcgttgc agataatctg
    14581 aaaaattgct ttgagaaacc tgatagtaat ccagaagaac aagcggctaa actagaacaa
    14641 gcgatcaaga tgattattaa aaagtaaagg aatgatttca atgaaagtcg ttttccatat
    14701 tgatgaatta gaaaaatggt cagaaacagg gaaaaacgta aaaaacctga taaaagcttc
    14761 tccagaaacg acgattgtcg tatctgtaaa tggcatcgcg attaccggtt atctagactc
    14821 tgccaatgca gaattcttag atttgcaagg agtcgttttt cacgcttgcg ccaatgctat
    14881 gcgtgctaat catatttcag aaagttcttt acctgaacaa gtaatcgttg taccagcagg
    14941 tgtccttgat ttagttgaac tgcaatccca aggttatgct tatatcaagc cttagtcagt
    15001 agaggttgcg acagaagtga tcagcttcaa gaaatttcgg cttatttttc gaagaagcta
    15061 cttctggaaa accgtgaatc aaaggttgtg aaaacgctgt cctgcatcaa gtaataagaa
    15121 cagccaaaca aaaatagctt ctcatatttt tcgtttggtt gggcttatta ccgcagatgc
    15181 aagcttttga acaccgttta aggaagaggt tgtgaaatca gcggtttgac ctaagtatta
    15241 gagaaaaaaa cgggaaaata gctcctcata ttttgccgtt ttttgagcta ataccgaagg
    15301 tcagctgatt gaacaccgtt taaggaagag attgtgaaaa agtcgcttag tttcgagtat
    15361 taagaaaaat tttgtgaaaa tggatttcta catttttgca taaatttggc ttaataccga
    15421 gaaactggct tttgaatacc gtttaaggaa gaggttgtaa aatcagcggt ttgacctgag
    15481 tattagagaa acaaacggaa aaatagctgc tcatattttg ccgttttttg agctaatacc
    15541 gaaggtcagc tgattgaaca ccgtttacca agaggttgtg agcctaccat ttaactccga
    15601 atcacaagaa ataatttcaa aaacaacttc tcctgttttt ggttcggttg gcttattatc
    15661 gagaagtagc tactgggagt ctatttatca aaaaaaggaa tgcgacatag gtcacacccc
    15721 ttttttattt tctttgaaac atctttcggt actcgacagc atacaagaca aaagattgta
    15781 aaagaccgta agtgatgacg gcaaaaatga ctctcaccca cagcgttggt aattctggga
    15841 aaagcagcaa aaggatcatg ccaacataga tgacagcagt cgctactttg ccataccact
    15901 tcgctccact taatttgata tttttagata gcccgattaa tccagctaca aacataaagc
    15961 cttcttttat caagaataga ccaaacaaca gccaaagcca cggtcgatac catgccatag
    16021 acaaaatcag aaaaagttgt gtcaatttat ctgcaaacgg atcaagcacc ttccctaagt
    16081 ctgttacctg attatatctc cgagcaatga ccccgtctag ataatcagac aaacctgaga
    16141 caacaatgac cgctcctgct atataaaatg actgtctcag cacaaaataa agataaatcg
    16201 gcagcaaaaa aatacggaat atcgataaaa gattcggaat cgtttgtatt tctttacgcc
    16261 agtctgtgct taatttcatt ttatcgccct acctcctcag cactcttttc tttttatttt
    16321 agcttacacg gggaataaaa ccaatgattt cactgttttt atttttcttt acagacactg
    16381 atattatgag ctaatttgag ataatcactt gctgttttca tcgtttactc ttctttcttc
    16441 ctgtcgtttg cgcatttctt ttactagtac tttatgagct ttttttgatc ctcgctctag
    16501 gtcacgctgc cgtttacgtg ttttataatc gataaacaga ctgtctaaat cgtaacctgt
    16561 cgggtacaga tcttctgctg aacgcaccag acgaacacgc tgacgaggca cgtggattat
    16621 ttttttgtct aaatatacat caatcgtatc ttcacctata tcttcataaa ctaacgcttc
    16681 tttttggtat tcattgacaa atatccgatc accttttgca aagagctgac gttctttttt
    16741 attctccttc ctatttgtaa ctgctgtctt aggaagaaat tcttttttct gtgtccgata
    16801 ggctttttcc gccaagtaac gctctgcttg atggatcaat ttcatcgaca tatccatttt
    16861 atgagcaatc cacaaagctt ggctttctcc tgtttcttcc aacccgcaac tgatatttag
    16921 gacgaagtgc ttcccgatcg aaagccatag cagccggcac aaaatcttca tgatctccgg
    16981 cgaatttctt gatttctcca taatgggtcg tggcaatgat caatgcccct tgctcataca
    17041 tagattccat gattgcgatc gctagtgcag ccccttcatt tggctctgtt ccactgccaa
    17101 tctcatctaa aagtaccaac gtattccttt ttatcttttt caaaatagca gcgatgtttt
    17161 gcatatgacc agaaaatgta ctcaacgcat tttctaaatt ttggtggtca ccgacatcga
    17221 cgaaaatctc atcaaagaca gcaagctctg tcccttcttt tgccggaact tgcataccga
    17281 acatcgccat caaagtcaat aaaccgactg tcttcaacac gactgttttg cctccggcat
    17341 ttgctccggt gataatcaag cctcgatagt ctttaccgat ttcaacatcc aatggtaccg
    17401 catgctcagg taaaaatgga tgtctgcctt gcttgataat gatatgttcc gatttattga
    17461 ttctaggtgt gcttccattg atttctcggc taaattttgc ccgagcaaag atgatatcta
    17521 acaccgtgat cgtttcgatc aataaatcga tcacttcttc gttttctgcg atagcaccag
    17581 tcaatgccgc caggacttga tattcttcac tgatttcttc agcttttaac aactgatagt
    17641 gttcattcaa tttagaaatc acagttggtt caataaaaac cgtcgttctt ttatttgatt
    17701 catcgataat cgttccatct attttatttt tatagctggc tttgatagga atcgtatagt
    17761 attcgccttt ttgaacaatc attgcttctt ggatcatttc tttgtttttc ggatgacgca
    17821 agaatttcag taattttgac tggatttctt tttcgattag ttgtaattgt ttgcgtactt
    17881 tacgcaaatt acgtgacgcg tcgtcactga ctttattatt ttttattttc tggtcaatca
    17941 actcttctac atttatcaaa tcaggaagat gtttgctata agcaaacaac aagggtgtct
    18001 ggtattgatt cttatcaaaa aactttgtga tcatccgact gcttctaaga aaatccgcat
    18061 attcgatcaa atcggttggc tgcagaatca accctttttt gacttgttct gtcagtgcat
    18121 cgatcctcgg caaccccata aatggaacat gctgactgct ttctaagatc aagcgtgctt
    18181 ctcttgtttc ttcttgacga tccaagacag tttgtaggtt tgtcgagacc gtcatatcgc
    18241 tgattcgttt tttgctatag tttccaattg ctcttgcttg gatttccttt ttgatccgat
    18301 caaattgtaa ttgactaatc atttcgttgt ccatattctc tccttttctt gaagagatac
    18361 ctgtcccaaa ataaataagg gcctagagat aaataaaagg atagaacaca gcttaaaaaa
    18421 gtcatgtcct atccttatta gaatacaaag atattgtttt cctgacaggt accgtttcat
    18481 aagcacacca aataaacttt gtttattggc tattcgactt actgattaga taccagctac
    18541 actcacaaaa cataccccat tcttctaggt cttatttatt ttccacttaa ctatagcaag
    18601 acacattttg gttgtcaaac tttagtatgt tttatagatt tacaccatta atgaatagac
    18661 acctaccgcg ataattgctc cgatcattgg acctgcgatt ggtacccatg aatatgccca
    18721 atctgaagtt cctttattag ctattggcaa taccgcatgt gctaatcgag gaccaaagtc
    18781 ccgagcaggg ttgattgcat atcctgttgg ccctcctaat gaaagaccga tggctaagat
    18841 caatccacct acagcgaata cgttcgttcc aggtgcaaat tcgttttgtc caaaagctag
    18901 caacccaaga actaatacaa atgtaccaat taattctgtg atcacatttg ctggataatt
    18961 ccgtatagca ggacctgttg caaacgttcc taaaatcgcg ctttcatctt tcgttgcatt
    19021 ccaatgtggc aaataagaaa gccaaacaag aatggcccct gcaaaagctc ctaaaacttg
    19081 cgcaatgatg aaaggaacga caagattcca actaattgcc cctgtcatcg ccatagctag
    19141 agagactgca ggattcaaat gagctgggcc catgaatcca gaaatataga cagccattgt
    19201 tacagcgagt ccccaaccaa atgcgatgac aatccagcca gaagcctgtg ctttactttt
    19261 gtttaaattc acagccgcac acacaccatc tccaagcaaa actaggatca gtgtcccaat
    19321 aaactccccc atgatttgag tcatatcact gttcatttat ttctccccct ttagttcccg
    19381 cagatctgat tctgcaatca atgcttctaa acgtttagtc tgttgcgcct tctcctcttc
    19441 tgtccattca aaatatccag caattgcatc gatcacaggt tgcttgatct catcaagctg
    19501 atctcgttca aacaacagat gatttgtacg acgaatgaga taatcgcctg gggccaaaac
    19561 catttcttct tctaaaccat aacgtaaccg agctgactca gcgagactca aaccaggata
    19621 aggtgccatt tcttttgcta attcaaagat acgtcgggca ttagtcccgt aaaaatcagc
    19681 gatataagta gcatcttctt cctctaaacc ggctgctact ccttcttttg ctaattctgt
    19741 cactgtttct tctaatttag ttggatcgaa gtttccacct gaaatctgat attttttaga
    19801 gtcgatttct ttcgtctcta ttccgtattc ttcttttaat aattggcgta tcaatcgcaa
    19861 agctccttct gccatcttac ggtaatcggt gattttccca ccagataaag tcagcaaacc
    19921 atctggttct ctttctaaag aactacctct ggaaatcgta gaaggtgctt tttcatcacg
    19981 gctgttttcc aaatggttca acacgtcttc tacttcagca cgagaaactt tgttttcctt
    20041 atattcactt actgtatcga caactttatt gaagctcttg tctgaaatcg atccattatc
    20101 tccaccattg taatcagaac cagaattacc aatcaacagt ggacgaagtc ctgcccagct
    20161 tgcttcgata tcattcaatg tgatatttgc ttctggatag cgatgattga tcacatccaa
    20221 tagataatct acatcttctt gtgtgacttt cggatccgta aaatctcctt ggtaatccgt
    20281 atctgtcgta ccaaagtaag tcttgttttc tcttggaata gcaaaaacca tccgcccatc
    20341 ttgctttcct gtatcgaagt atgtgggttg cggtacaggc agttttttcg catctacaac
    20401 taaatggatc cctttagttg gacgcatttt aggagagact ggacgcgtaa aattcaagtt
    20461 tcgtacttta tctacccaag gaccactcgt attgatcact aattttgctt tgatttcgat
    20521 cacttcatct gtcagcaggt cacgagcttt gacgccaacg atttgatcgc catcatataa
    20581 aaagccaacc gctttcattt tacttactag ataagcccca tcttcggcag cctttttgat
    20641 attatcaatc actaaacggg catcattgtt gcggaaatct agatagacac ccgcgccttt
    20701 tagcccttct tttttcaaaa acggttctct ttccaatact tcttctggtg tcagcgtata
    20761 attctcatat ttggttcctg tcacatttgc cagtttgtca taaaggtcca ttgctacttt
    20821 gaccgagaac atattgaagg ttgttgctcc ttcgccctca tagattggca aaagcattgg
    20881 atctggtttt gggatgtgtg gggcaatact ttgtacgact gcacgttcac caactgtgtc
    20941 agctactact tccacatcaa atgtcttcag ataacgaata ccgccatgaa caagtttggt
    21001 cgagcgagag gaagtccctt ctgcaaaatc ctgcatttcg atcaatcctg ttttgattcc
    21061 tgatgctgct gactggactg ccacaccggc accagtgatt ccgccaccga tgatcaacag
    21121 atccaattct tcttgctgca ttttttgaat gctatcttgt cttgtcttgt ttgaaaacat
    21181 atggatttcc tccttattca aatgcttggg tagcttttac tgcttttttc caaccgttgt
    21241 aaagtttttc tcggcgctct tcctccattt gaacttcaaa tagcttgcca gcttcataaa
    21301 attcgcggat ctcgtctgta tctttccaat aaccgacagc taatcctgct aaaaatgctg
    21361 cccctagcgc cgttgtttcc aagttttctg ctcgttggat cggtacattc aagatatctg
    21421 tttggaattg catcagatat tcattgtttg ctgcaccgcc atctactttc aatacaggaa
    21481 tcttgattcc agtgtcatct tgcatcgtat cgatgatatc tctcacttga taagcaatcg
    21541 actgtaaagt agctttaatg atatcttctt ttgtcgtacc acgtgtcaag ccaaacatag
    21601 cccctcgtgc tgcctgatcc caataaggag cacctagccc aacgaatgca ggaacaacat
    21661 aaacctcatc ttcgcttgta gagcgtttcg ctgcgtcttc tgaatcgcta gctttttgca
    21721 acatttgaag tccatctctc agccattgaa tcgaagaacc agccacaaaa atacttcctt
    21781 ctaaagcata gtaaaccttt ccatttatgc cgtaaccaat tgtcgtcaat aaattatttt
    21841 tggataattg cggtttttca ccggtattca tgacaataaa tgaacctgta ccataagtat
    21901 tcttgaccat gcctggttcg aatgccattt gtccgaataa tgcggcttgc tggtctcctg
    21961 ccatccctga aattggtgct tcgcttccat aaaaatgata accttgcgta taaccataaa
    22021 cttctgagtt agaagtgact tttggtaaca tcactcgagg aatatttagt aaatctaaaa
    22081 tctcctgatc ccaatctagc tgatggatat tgaacaacat cgtccgactg gcattcgagt
    22141 aatcagtaac atgtgcttgt ccatcggtca gtttccatac taaccaagaa tcgatcgttc
    22201 caaacatcaa ttcgcctttt tcagctcgtt cttgcgcgcc ttctacatga tcaagaatcc
    22261 agcgtatctt cgttgctgag aaataggcat cgatcactaa ccctgttttt tcgtggaaaa
    22321 attcttgata gccttcattt tttaagttat ctgcaattcc agctgactgt ctggattgcc
    22381 aaacaactgc atgatagatt ggacgtcctg tatctttttc ccatattact gttgtttcac
    22441 gctggttcgt tatgccaatc ccagcaatct gatttggttt gatccctgat tcgatgaacg
    22501 ctccggcaat aactgactgt acagaattcc agatttcatt tgcatcatgt tccacccaac
    22561 tttcttgcgg gaaatattgc gtgaactctt tttgagaact gccgatgtgt cttcctttct
    22621 tgtcaaagat aattgctcgt gagctagttg taccttggtc aatcgccatg atatacgttg
    22681 attctgtcat tgtgattccc tccattatat gcaagcgctt tctttaacta ctcttatcat
    22741 actactgttt taaaaaaaga aactgtcaaa attttattct ttttttcttt ttttgacagt
    22801 tttttattat gaagctattt tttaatcaat tcttttcttt tttcagcgtc tctattcttc
    22861 ttttcaatcg aataatgtcg taaggtgacg caaattctga cagataatat tcacactgtt
    22921 ttttgtttct ctggcaaaat gtgatcacca aatcatattc tttaccttct tcataacttc
    22981 caatctcaat accgccgatt cccctcagtt ctctgatcag atattgttga aaagcaatac
    23041 gataaatcgg cagactttct aaatcgattc caatcactac tggttttgca atataaaaat
    23101 cgatcatcaa aagaacattg gcatactgga tcatcaggta actaagctca ctatcttcag
    23161 ctcttttcct cggcaattgc tgcaccgctg tttcctgtaa tttcgccagt aactgtgtta
    23221 aggaatgccc gagcattttc tgctgctgtt ccaaaagccg atcacgctca tagacttcga
    23281 tctttccaac aaagaaaaac cattcattgt tgatctggga aatttggtag cctactgcct
    23341 tttcttcttt gattttcaaa cgacgaggac gataatgcca taacatcgtt tcccgaatgt
    23401 aagtatctaa aacagcggtt ggtaactttt tgctccgtgt caaatcatac tgataaaata
    23461 tttcttcgtc tactatcgaa aaagaaacaa aaaagctgta aaacatcatt gtttcgtata
    23521 cattcagttc tgctgctgtt cgactaagat gcatcacgat gattggatcg attgcttgat
    23581 aaagccggtc cgactgatag agtaatttct tttctttcaa agttgcgtac gtactttttt
    23641 cattcaatag tctttttttc gtaatcccga gccagcaagc aatctgtgct tctgctgatg
    23701 ccgtaaacgt tgtttctaat acacggtcca atcctagaat gaacggtctt ttttctggtg
    23761 tattttgcaa aaaaagcggt cgctgatctt ccgggatata ttggaatagt tcataataaa
    23821 aatatcgtat ctgtagctct tctccgtata attgaccgtt tttgatttgt aatccaaatt
    23881 cctctaagag ctggttcaat tctttgatct ttcgaaaaac agaagactca ctgatattaa
    23941 acgctgtcgc taatcgaacg atggatactt gctgatgttc taataaatag acgagcatct
    24001 taaacttcaa gctttcctgc aataaaaaca atagaatttt ttccaaactt acatcttcgt
    24061 ccaacacaag cagcgcctga tactcatttc gctgtacttc tacttttttt tccatcattc
    24121 ggccaatcaa ttcaatatct tccaaatatt gatcaaacga ggcttttgac agttggacat
    24181 gctctctcaa accattgata gaggctgttc ccccttgcag aacaaggttt cgcaaaatca
    24241 ggatctgtaa ctgctctttt ctttctaata atgcttcaat cttcataggc agtattcgcc
    24301 acctttgacc acaccatatc tggcacacgg tattctggct ggaaaaatag ctcatgttct
    24361 tctaactgtt caaacgtcca tccttcttct actgcaattg tcagttggtc aagaaggtgg
    24421 aacatctctt ttttgctttg gagctgtgct ccaagaatct ttttttcaga atcaaataga
    24481 aatttgagtt taactttgct ttgatttatt gctgtaagct tgtagtctac gtatcctgtt
    24541 tcaatttttt ggggataaaa aatgctctct tcttctgtca aacctacgct tcctaagaaa
    24601 tagccaaacc actgattgcc gactggacga taggttccct gatcctttgt ctgtgagcca
    24661 ctaattgtct tgctgacatt tcgtgctgta cggacagcgt tatttactag agaaacatac
    24721 attttttcat ttgttggacg aaaagttact tggatcgcat cgccaatcgc ataaacgtct
    24781 ttttgagacg tttgcaagta gtcatctacc cagatcgttc catcatcatt caatgttaat
    24841 tgttcttccc acatctgatt gtcgggacga gaatgattag ccagcaaaac aagatcatag
    24901 ttctcctttc ttttttccgt ctctacaata gcggtttcat tttcggtcaa agacacaact
    24961 tgttcttcta aaaaaagtga aagcccttga tttttcattt cctttactaa cggttctgtc
    25021 atttcaggat ctagataacg aaaaagaatt gactttcggc tttcgtaaag atggatttgt
    25081 ttttttcttg atgccaaccc ttcagctatt tcgattcctg cttgtcctgc accaataatt
    25141 gcaattttct ttgcattcat gatcttttgc tgaagagccg ttacgttttg tatcttttta
    25201 actgggtgaa taagcgttga ttctgcagaa atatttcgaa acttttgatt tgaaccagtc
    25261 gctagaatca aacgatcaaa atccaatttt tttcctcctg ctaaaacgac ttttttttct
    25321 ttcaatccta ttactgtctt ttctgtgtaa acatggattc gatagcggtc agtcagttct
    25381 tctttcgtga tccaagaaag ttgtttttca ctggtaacct ttccttttaa tatcaaattg
    25441 aacccgctgg gaatccatcc taacttcgat tgcttttcaa tcaaatatat ttcacttgtt
    25501 ggcatcaatt ggcgaagata aatagccgca tgaatccctg caaacgaccc gccgacaatt
    25561 acaatgttca tctgcattcc tctttcttat aaggtgttct actgctaact tctcaatcag
    25621 aaaacacagt attcataact cgctttcaac cttatgatac atagcgatgc agcagaatca
    25681 accctctcca tcataccgtg ttctaggtct gcttctcaat aatgtactag tggacctaat
    25741 tgaagagtat catttctgaa gagatgtttc aaaaaatgta ctcttataaa agaaaaaaac
    25801 tcgcctttta cgtacctctg tccaaatagg aaacagaaaa gaacgaaaag acgagtttta
    25861 gcgtgtgtaa tgtaatagtt agaatagaca catcatttat ttttaattct ttaatgcttc
    25921 aacaactgct tcgcctaatt gatgagcaga ttgaggattt tgccctgtaa gaagccgccc
    25981 atcaatttga accacttctg aaaaagctgc tccagactga tagtttgctc ctttttcttt
    26041 caaagcatct tctgctaaga atggtacttc attcgttgta ccatttgctt cctcttcttc
    26101 attactgaac ccagtcactg tacgtccagc taaaatagat tttccagact catctgtaac
    26161 tggcaacaat cctacaacac cgtgacaaac agctgagacc agcccttcat ttcgatagat
    26221 ttgtgaagcg actttcccaa gatctattgc ttctggaaaa tcccacattg ttccgtggcc
    26281 accagcataa taaaccgctg cataatcatt tgcatccaca tcatcaggac gtaaggtatt
    26341 tcccaaggcc cgatttctga aatcttcatc ttcataatag gcccaatcga tttcatccat
    26401 attcaaaaca ctttgcggtt ctaaaggtac atatccgcct ttaggactga caaaatctgc
    26461 atcgatccca ttttttacta atacatcata aaaatgagtc agttcactta accacaaacc
    26521 tgtaggacgc tcgatctttt cgtacttttc tacatttgtt aatacgatca atactttcat
    26581 tttcccatcc tctttctata aaaattgcgt tattgataac gtgctttcaa gaaattatat
    26641 aatgggcttg gcaaataaag ctctttatat gctttttcaa aaggtttcgt tacaaattca
    26701 tcctcacggt tttccaataa tttctctgcc cagtcaggat cgacgatcat ttgctgtcca
    26761 attgctgcaa gttctgcata tttcaatacc tgctctacat cttcgcgatt gcggatccca
    26821 ccgacaccaa ctaaagggat ctttccttga atcgtgttat ggaccacttc aagaatcgat
    26881 ttatcgttgt atttatcaga acgagctatt cgatcataca tatttaatga aacatgcaga
    26941 taatctagtt ttgactcacg taatcgttca agaagccaga tcgtgtcttc aaaacggata
    27001 cctggtgttt catattcttc tggtgagaaa cgatacccta cgatgaatgg ttttttagca
    27061 tattgttcaa ccgctgagaa aaccgttgtt aataattctt caataaaacg ataacgattt
    27121 tccaagctac cgccccattg atcctctcgt ctattcgaat gcggagagaa aaattgttgg
    27181 atcaaatacg tatttgctcc atgcaactcg attccatcaa atcctgattc aatcgctcta
    27241 cgtgtggctt tgccaaatgc ttggattgtt tcaacgattt cttcttcact cattgctcgt
    27301 ggtgtttccg catcaggacg ttttgctgca actgcacttg cagatacagg ctgttctcca
    27361 gataaagttg ctcgacttgt cattcgtcca ccgtggaaaa tctgaataat cgcttttgcg
    27421 ccttctcctt ggattgcttt cgctagctca cttaatcgag gaatcatttc atcatgagcg
    27481 atgctgagtt cacctggcca gccttttccg atatcttgga catttgcagc accagtaata
    27541 acagcaccaa gacctttaga acgttgttta taataagtga tctcatcttc ggtgatcact
    27601 ccattgaaaa agctgagttg tgtcgtcata ggcgacagca tcaatcggtt cttcaattga
    27661 actcctgaag gtaaagtaat tgattgatca aattgtgaca ttgtcattcc tcccataatt
    27721 cgctctgatt aacctatcga gaatcatcta tcattaaacc actcgattag ttgtacatgc
    27781 aactaatttg cttaaaaaat tttatttttt ttgtaactta tctgttgcat cattcattgt
    27841 ctgggctaat tctttacttg cttcatttcc caaaactgct ttgtatgact gatgaagttc
    27901 ttcccagcta ccatcgactt ccttagcaaa ttggcgtcct tgttctgtaa gataaactaa
    27961 tgacaagcga ccttcgctaa ctgttttgac gtatccttta tattctaatt tttcaacaaa
    28021 acgagcaatc gttgatgggg cggaatagag catttgtgca atatcttttt gtggtattcc
    28081 atctttttct tttaatatca ataaaataaa accataagtt ggtgccagac ctgtccttct
    28141 aaacgcttca tcagctaatt tattcaacat gcgatctagc tttttcacgc taaagaaaag
    28201 acattcatct aacgcttttt caattggctt ttccatttca tcccctccat tggcgtacat
    28261 atcatagtat gatttttatt ttttgtcaaa aaacacggaa taatgtccgc ctcctctgtc
    28321 tagatttgaa aaaaagaagt agccatcttc aaagaatttc ggcttgtttt cgacaaaact
    28381 ccttctggaa caccgtgaat cagaggttgt gaaaaagtcg tttagttttg agtatgaaga
    28441 aaaattttgt gaaaatggct ttccacattt ttgcataaat ttggcttaat accgaaaaac
    28501 tggcttttga acaccgttta tatcagaggt tgtgaaaaag ccgtcctgca tcaagtaata
    28561 agaacagcca aacaaaaata gttcctcata ttttgtcgtt ttttgagcta atgccgatgg
    28621 tcagctgatt gtacaccgtt tattgagagg tcgtgagcct gccgttgaac tccgagtcac
    28681 aaggagcaat ttcaaaaaac agcttctcat gttttatgac tatggtgact tgtgatcgag
    28741 gagttggcag gagaatactg tttattcgta aagaaggggc tgtgacaaga cttttgtcac
    28801 agtcccttat ttccttttat tataggattt cgccatttga ttggatgact tgtctgtacc
    28861 aatcaaatga ttttttcttt aaccgtttct ttgtgccatt cccttcgtca tcttgatcaa
    28921 catagatata tccataacgt tttgacattt gtgaagtaga agcgctgacg atatcaatgc
    28981 agccccacga cgtataacca aataaatcta ctccatcttt gatagcttcc ttcatctgct
    29041 caatatgctg cttcaaatag tcaatgcggt aatcgtcttg aacggttcca tcttcttcta
    29101 ctttgtctat agctccgagt ccgttttcag ctatgaacag tggtttttga tacctatcat
    29161 ataattgatt caaagcaatt ctcaaaccct taggatcaat ctgccagccc cattcacttg
    29221 atggaagata cggattttta attcctccag ttattaagtt tccttggacc ccttcccaat
    29281 tttctggatg agcactgact gcactagaca tgtagtagct aaaagaaaca aaatcaacag
    29341 ggtaggctgc taagatttct tcatcaccta cttctatttt taagttgaca cctttttctt
    29401 caaacattcg ttgcgtatac gctggataat atcctcttgc ttgaacatca ctaaagaaca
    29461 aagaagttct ttgctgctca attgtttttt gaatatcatc cggatcacaa gaatacggaa
    29521 caagaagctg atagctgatc atacaaccga cttgtgaacc tttgataatt tcatgacaag
    29581 cttttgtagc taaagcactt gctataaact gatggtgtgc accttggtat ttcgcttgtt
    29641 ccaaatgggg atggttttct tcaattactc ctacgctaac aaaagggtga tgttttacac
    29701 agttgatctc attgaaagtc atccaatatt ttactttatt tttatagcgt ttgaatactg
    29761 tttcagcgaa acgaacgtaa aactgaataa gctttcgatt aggccatcca ccatattctg
    29821 taaccaaata taacggcatt tcataatggg aaagagaaac gactggctca atcccatatt
    29881 ttagacattc gtcaaacact tgatcataaa aaaataatcc cgcttcattg ggtgtttctt
    29941 catcaccatg aggaaaaatt cttgtccatg cgatagacat ccgatagcat ttaaaaccca
    30001 tttcagcaaa taaacggatg tcttctttaa agtgatgata gaaatcattt ccgtgtcttt
    30061 taggataata atacttgctg ttgggatcct gcgccgcttt tacttgctga tgcgtcatca
    30121 tatgccattg atctgtccat ttttctttag gtaaatcgct gtcgaatgta tacacatcag
    30181 acacagataa tcctttttca tctacatccc atgcaccttc acattgattg gccgctgtcg
    30241 ctccgcccca taaaaatcct tctggaaatt ttttcatatc gatcccccta aacttgctag
    30301 ttttcttgtt tgattatttt tcttatgact agtaatgata tttttgaatc tactactatt
    30361 tgtaacaata accattgtgg tcggatcata tccctctttg attaattctt gcaaatctac
    30421 ttccatcaat aaatcgcctt tacgtacttt ttttccatca gatactgcac tttgaaagta
    30481 tttgccattt aaattcactg tatcaatacc gatatggatc agtatctctg ctccttcttc
    30541 actgactacc ccaattgcat gtttcgttgg aaaaactgtt gaaacttccc cgtttacaga
    30601 actgtagatt tttccgtccg ttggaatgat cgcaaaccct tctcctaaag atccactagc
    30661 aaatgttgga tcgtttactt cagataaatc aattatttca cctgctgcag gagtacttaa
    30721 cactatatcc tttgaggatt cttcttcgat ttcatccttg tagccgaaaa gaaaagttag
    30781 catacaggaa ctgatcatcg ctaagaaata actagcaact agccaaacaa atccttcacc
    30841 aatataaatc gggaaagaca aaatacttct tgtagcaact gccaaagcac cggctcctgc
    30901 ggcaccagct actccaccaa atatagctcc acatatacaa gcaatgtaaa atggtcgttt
    30961 atatttaaga gtcacaccat aaacagcagg ttcagtgatt ccaaataatg ctgtgataaa
    31021 tgcagataaa gataaacttt ttactttctt atttttcgtt ttcaaaaata ccccaagtac
    31081 tgctcctgct tgcgcagcta cggcagtcat acatgctgga cctagcgtat cacgaccgta
    31141 gaaagaaaga ttgttggtca ttactggtac aagtccccaa tgtaacccaa aaatcactaa
    31201 cacttgccaa aatccgccga taacagcacc agataaaaca gggctaaagt tataaacagc
    31261 taaatatcca gctgcaatca gctgacttgc ataatctgca attggaccta caactaataa
    31321 aataattggg accatcacga tgatacaaat gaatggtgtc aataaatttc gaatactttc
    31381 gtgcaagctt ttgcgaatct taggctcaat ataagaaaga acccatacag ctaaaattgc
    31441 tggaataacc gtacttgtat accttgctag aatcacgggc atccctagaa atcgcaaggt
    31501 tatgctatca ctatatgcac tgacaatcgt aggataaacg agagctgctg caataaccat
    31561 agcgatgaaa cgatctgtat tgaacttttt tgcagcagta tatgccaaaa atataggcaa
    31621 gaaatagaaa aatgcatcag ctgctgcatt caaaatgata taagttcctg attcagtagt
    31681 taataaattc aaactcgtac ataaggcgag tactcctttg atcaaaccac ttcctgccaa
    31741 gacgcctaaa gtcggggcaa aaacagcggc gatcatgtct ataaaagagt ttaaaatgcc
    31801 ttttttctca gttcctctct ttccactaga tgcatcatct tctagtttca tttgtttgga
    31861 taatacgtca tacacttcat ttactgcatt tcctactacg acttgaaaca ttcctccagc
    31921 ttcgactaca gtgatcacac cttctagttg ctctaccttc tccttatctg ccttcttatt
    31981 atcttttagg gtaaagcgta accttgttgc acaatggtaa acattgctga cattttcttc
    32041 gcctccgaca aatcggacaa tatctttcgc taattgtgaa tagtccattt ttacttcccc
    32101 cttgttaatt aatgccgtag caatccttct tacaagataa gtatagttct tagaaaacgg
    32161 tttctccatt caattagtac cccttctaat agcgaacgaa actgtacaat agtgtgtatg
    32221 gtttacttag ccgagcaaat cactcactgc ttgaaagaaa acaagtatat cgtttctctt
    32281 atagtcaaag gagacaaaaa aaacacgctt gatcttcctt tgctgatgta taagtggtat
    32341 ctcaagctga tactctgtga tttttgtttc tacttggctt ttgataagac agcacaacaa
    32401 agtctcggca tttaacggaa gcataactag gtcagaacta ggagagatta gttgctgaat
    32461 ggaatctatc tttttatgga tttttatttg attttcttct ttgtagtaat gatcaagcag
    32521 ttttgttttc tttttttctt caactatggt cagcttttca cgcaaaatct gataaatctc
    32581 ccaagattca aactcatcta gaacactagg tagataaatc aaatcatccc actcaaaaat
    32641 aagctcttgc tcagtagtca aaattgcatc atattccgtt attttgtttg attgattttt
    32701 gaaaagatac actggttcta tccggcattc tttgatccac atgttcatac tttgttgaat
    32761 ctgttttttt agtgtattca aaatactaat tggctggtta ctaacaatca gtatctcccc
    32821 ttgttttcga aaaggattca acgcttcggc aagatacaaa acaaaataag cgctttcagc
    32881 taaattggga cgtaattgaa aatgtattgg gaagaaacgc cgaattaaat aaatttctaa
    32941 aggataacgg gagatgatct tttgtacaaa atgattcatg gtattatgtc cggcatccat
    33001 cctcgttttc atttgtttga tatgggttag caaaaattct gtttcatgga acaataacga
    33061 aacaggaaga tctaaaaaac gaataatcgt ttgctcaaat gctcttagat tcttctctat
    33121 ttctttgttt ttttcatcat taatcatcaa ataatttaac tctaatagtc gattttcaat
    33181 tgcttgtttt tcttgattgg aaaaagaata gggtagttct gatgataact tttttgtgag
    33241 ggtctctacc atcggaataa ataatgtata cctgtttgtt ttttctaaaa gttcttctct
    33301 tcgaagaatg gtcaaaacga gataccgaga aaatcgtatg aagtcctctc ctgaaacaat
    33361 ataattatat gatataagaa cctcttttaa tgtcttttgg acagtcggca tgatattgga
    33421 aaaaattgta ctgatttgtt gaggaaacct tgcgtgttgg atcaccgttt ctgttccaac
    33481 aagagataag aaaattcttt ttgaattttc ttcagcaatg atttttagtc cttttgtcga
    33541 cgaaacttcc agttctaatt taggattgcg ctcgatccaa cgcaaaatcg tttttatttg
    33601 taatgatacg gctgtttttg aaagaaagaa aatgtctgct atcttttgca tactgacata
    33661 ccctttttga aacaataagt acgtcacaat agttgaagct cggaaatcca tgctggaata
    33721 gattttatgt ttcgtcacct cttgtatgat ttgctgttta gtttcagatt caattgattc
    33781 taaaaaataa cctgaacgtt tatttgactg gataatgatt ttcttctcgt agatagcaag
    33841 catatttttg atttcagttt ggatcgtttt cttgttggta cctaaagctt cagctaattc
    33901 ttcgcttgtt atccaatgat tcttatttct taatactaca agaatttgga gttgtcgttt
    33961 atttaagtcc atttgcttac acctcctctt tgttataaac atacaggaaa aacctctcaa
    34021 atgacaatag tcacttgcag aggtttaaaa atcatttgaa gaaagatcta tattcaaggt
    34081 ttttaccatt agactggata atttgtttat aatattcaaa actatctttt ttcaatctcc
    34141 taaagcttcc gctcccatca ttttgtctat ccacataaat aaagccatat cgcttcctca
    34201 tttcccctgt tcctgccgat acgatatcga ttggtcccca ccataagtaa ccaatgattt
    34261 ggcaaccatc ttttattgcc tcagcaattt cctgaagatg tatttttaga tactcacaac
    34321 gttttggatc gtggattcga ttcaattcat ctggtgcttc atctagtcca atgccatttt
    34381 caactatgaa taatggtaat tcataacggt ctgtcaattc attcatgaca taacgaaacc
    34441 ctagaggatc aattggccaa ctccaaggtt cgggagattt ttccttcaaa aagggattat
    34501 cgaaaccatt tgctccgcca gtatctacgc gcatttctgc ttctttttta tagacagcgc
    34561 tacgataata actaaatgca atataatctg ccgtgttagc ttgcaagcac tgttcgtctc
    34621 catctaacat tactggtgca cagtcttctt ccttccatgt cctgtaaata taagctggat
    34681 aatgccctcg gcacatgaca tcggagaaaa accatgaatt gcgtttaaat tgcagtgaac
    34741 caaatacatt ctctggatca cagtcatatg cataagtcgc taatgcagaa aagctacaca
    34801 taatacccat cttagcattt ggatcgattt tctttaataa ttgagtagca cgggcgttcg
    34861 ctagaaacat atgatgacag gcttgataaa tcactttttt tgttagtgta gcaatgggtt
    34921 caagtttatc tctagcaggg aatgtaacca atccagcagc tgcataaggc atacgaaaag
    34981 cattattgat ctcattaaag gtcatccaat atttcacttt cccttggtag cgttcaaaga
    35041 cggtctgcgc ataaaattca aaaaagtcga ttaattttcg attttgccac cccccatact
    35101 ctcctaccaa tgctaagggg gtttcataat gactaagagt aattactggc tcgatatcat
    35161 atcgatttaa tgtattgatt agtctgtcat aaaatacgag accagcctca tttggcttct
    35221 gttcgtctcc tcttggaaat attcgtgccc atgagatgct tgttcgaaaa gcttttaaac
    35281 ctaactcctt cagtaaacga atatcctctt caaatcgatg ataaaaatca atcgcttcat
    35341 gagaaagata atggagtgat tcgtctaaag caatctccca aatctttttt tcttcattcc
    35401 actgaatact aggatgctgg ttcatgatcc cgataagcgt atctgtcaca ttaggtaatt
    35461 tgccgtcttc caaccatgca ccttcacatt gattagcagc tactgctccg ccccataaaa
    35521 aattttctgg aaatatttcc tgatccataa ctatctctcc tcgctatcaa caatttctta
    35581 cttgaagtat agctcgattc atcaaaacaa gatattctaa ctatttcggc agaagagaag
    35641 aaaaaaattg aatgctactc ttcattttta atatttatat ataacaaata cataagacga
    35701 tccccgaaag aaaacactcg ttaaccatct aagcaaacga tttattttac ttccaacaca
    35761 aatgcttcat aaggtcgtaa atcaactttg ccgtctggta actgtttcag aggataattt
    35821 gacacaataa cccttaaagc ttcacgtgga atagtgaacg tctgcttttc actggacata
    35881 ttacaaataa cgagccattg ctggtcttca tatatgcgag aataggcaaa gacatgctca
    35941 ggcgtctttt ccactaattc atagtctccc cagacgatga tgtcatgttt tctacgcaaa
    36001 tcgatcaatt tcttgtacat ataaaaaaca gaatcttgat ctgctaacgc ttcttctgca
    36061 ttgatttctt tataatttgg attgactcga agccatggaa ttccagtagt aaacccagca
    36121 tgttcacggg cgttccattg cataggcgtc ctagcattgt ctcttccttt cgccttgatt
    36181 gattccaaga tttcctcttt tgtaaatcca gaagaaatcc tttcgttata catattgatt
    36241 gtctcaatat cttttgcttc cgcgatatca tcgattgggc aattcgtcat gcctaactct
    36301 tctccttgat agatataagg ggtgcctttc atcatatgga gcaagatagc aaacatcttg
    36361 gcactttctt ttcgatacac atcgtcattt ccccaacgag aaacaattcg cggaagatcg
    36421 tgattattcc aaaataaact attccatcct tcatcaccta aagatgtttg ccatttggat
    36481 aatacttttt tcagttcaga gatactcaag ggtttcaaat cccatttgtc tttgccttct
    36541 tgctgatcta atcctatgtg ttcgaattgg aagaccatgg ataattcatt tcttttagga
    36601 ttggaataaa gtttagcgat ttcggtagtt gctccccatg tttcgccaac agtcatcaga
    36661 tttttgtcac caaaagttgc ttgattcatt tcctgaagat actcatgcag tttaggtcca
    36721 ttacttgtga tttctttatc cggttctttg ccaatcatat cgatcacgtc catacgaaag
    36781 cccccaatac ctttatcgat ccagaaattc atcatttgat aaatctcttg gcgaacctgt
    36841 ttatttttcc aattcaaatc aggttgacgc ttgctataca agtgcagaaa atattgcccc
    36901 gttttttcat ccaactgcca agcagaacca ctgaatattg agcgcaatct attaggctct
    36961 tttccattga ccggatcacg ccaaatataa tagtcccgat aaggattttc tttattttta
    37021 cgggcctcaa tgaaccacgg atgttcatca gaagtatgat tcaccaccag atccatgatg
    37081 atacggatat ttcttttctt tgcttcttca atcagttctt ccatttcttc catcgtaccg
    37141 aattctgcca tgattgcttg ataattactg atatcatacc cattgtcatc attcggagac
    37201 tcgtatactg gagaaagcca aattgcatca atacctaaat acgccagata atccaacctg
    37261 gaaataatcc cccgcaaatc tccaatacca tcttgatttg aatcttgaaa acttcttgga
    37321 tagatttgat aaacaactga atggtgccac catttttgtt ccatgatttt cctcctataa
    37381 atggatgttt ctattttttg cttaatcatt tattttagtc tctctcacta aaaattgtaa
    37441 cgctttcaaa tagctattgc accttgttat cggtaacaga ataaaacaaa aaaacagctg
    37501 aggaaattcc ctcaactgtt ttttgaaagg attgatttat gcagggaaga atgctgattt
    37561 cacaacttct ttataaacgg tgttcactca gctgtccctt ggtattagct caaaaaacgg
    37621 caaaatataa gaaactattt tttcgttttt ttctctaata ctcaggccag aacgctgatt
    37681 tcacaacctc tttatttcac attcaatatc cacatgaatc ccacaaatac aaagaacaga
    37741 ccatacatga ttggagatac ttcttttcct cttttagctg ctaacatggt aattggatag
    37801 aagataaaac ctaatgcaat cccatctgaa atactataag tcaaaggcat gccgatcaag
    37861 atcaagaatg aagggatcgc aatctctaag tttccccatt cgatttgtcg caaagattgt
    37921 gccatcaata ctcctaccat aatcaatgct ggtgcagtta cttgtgaagt aacaactgat
    37981 agtaatggag aaaagaacaa gcctaacaag aagaaaatcc ctgtagtaat agctgtgata
    38041 cctgaacgtc ctccaacggc gataccagct gatgattcta caaatgcacc aacaggagaa
    38101 gtaccaaata aggaacccgc aagcatagct gttgaatcag aagctaaagc tttccctaca
    38161 cgaggcatct tgttgtcttt catgaaacct gcttggtttg ctaatccaac taaagttcct
    38221 gctgtatcaa agaaagtcac aagcaagaat gtcagcacta cgacccacat ttgaagagaa
    38281 ttgatgtccc ctacatgttt taatgcaact aaaaatgttg gtttcaaact tggtgctgcg
    38341 gcaacaattt cagaaggcat tggaatcaaa cctgtgacca gacctaaaat cgttgtagct
    38401 gccatcccga tgaagatgcc acctggtaca cgccgaacaa gcaagatagc agttacgact
    38461 aatccaaaaa tcgtcaacca agtagaacca acgttgagtg atcctaatcc tactaatgta
    38521 gattcattcg aaacgatgat cccaccttcg ctcagaccaa ggaaagcgat aaatagaccg
    38581 ataccgccag aaatcgcata tttcaaatct gctgggatcg catcaatgat catttcccgc
    38641 agcttgaaga ttgtgatcaa aataaaaatc aaagaagcaa cgaatacgcc ggctaatgct
    38701 gtttcccatg gaatccccat tcctaaacaa acagaataag caaaaaaagc attgatccca
    38761 agagcaggtg ccgttccaat tggataacgg gctaataccc ccattaaaac acagccgatc
    38821 gcactggcta atgcagtcgc agtaaacact gctccttcat ccattccaga agcacctaat
    38881 acagttggat ttacaaataa gatataagcc attgagataa atgttgtaaa tcctgccagt
    38941 acctcacgtt ttaccgtggt gttcaatttt tctatttcga agtaggaaag tattttttcc
    39001 ttcatcttcc atccccctaa atccgaacat tataaataaa gtccgttaaa atttcgattt
    39061 attaataaaa caacgtgttt tatcatatac taaaacaaaa cataagtaaa caaaaaattc
    39121 ggattttaag caaaatccga acgttccatc tgattaaatc cctttatctc aaagttcatg
    39181 aacattcact ttggattaca agaatttctt tattgtgtta ataaagtttc ttcattctag
    39241 tctcaaaaat caatagtgtg atttgatttt accatttcgt ttctgttctt ctttgatgaa
    39301 ttcttttgta tcgacaggat tcagattatt tagtccgcca aaacctgtat cgaccatttt
    39361 tccccagcct tttgtcttct tttttcctga tttgagattc tttgctcgaa gcttctcact
    39421 atgttttgct cgccgatact tcaaataacc ttctaatctt cccattagat ccactgtttt
    39481 tcctcctttt tattcaatca gacgactcag cttttcctat aacaaaataa ttgccttcag
    39541 ggtcagaaaa attgaaagtc aagatgccat tgttatctgt gatttctcct ggttcttcca
    39601 tgttctcgta gatagtttca aattcatccg taaaaaatac taaagaaggc ggtggtccta
    39661 atacctctgg tgaaaacttc tgcacaaata cttttgggaa tacgcctaat tctaattctt
    39721 ttgagatgct gagtacaata ctcttaaatt cttctggcaa ttcgattgtt tcagaaattt
    39781 ctgcaccaat tttttctaca aagaatcggc aaatcatctc tacgtcattg acataaagca
    39841 tcacacgtgt tttttgtatc atcatttgac ccttctttct atttgatttt ctttcctgtc
    39901 tataaactga ttccattata tcacgaaagc gcaaacacct tgtgtttgat actattcaag
    39961 aaaaataaag caatagtttc taggattgct ttcctccaca aacttttttt acaaaaaaaa
    40021 aacggcaatg aatgccgctc ttcttatttc gattctttta taattctgct tctgattcat
    40081 ctttggttag tgtgtgtcta acactagtta cgccaaatgt ttgatcgatt ttttccttca
    40141 acgcttcgat tgcctgtgcg ccacgtgccg cttgttcagc attgatggat tcaatgacca
    40201 gcaccgctgc ttgtgtttct tccgtcagca tcgttctttg ggcttcacgc cagttgagac
    40261 agcgacagat cgctccttgt tcatcatagt agatgatttc ttccggaagt gccggagcat
    40321 cggaatctgc acctaaaggc aggaatgatt cgccaccttg ggcttttcct aaatgcagat
    40381 ttcctgcgat cttttcgata tcttctccgc cgcaaggtac gccaaaagac aaggaaatac
    40441 tattatagat atctacaagc ggattgattg ggaaaaattc ccgttcttga tttgcacgtt
    40501 tcagtaaagc ttcaatagaa gaacgtgcac cttttttagt cttgaatgtg gtaaatgcct
    40561 gacgccactg agcaatgact tcgttttggc tgaatgtttc ttctgtaagg taatttcttg
    40621 cttcttttgc tgcctcattc aataattgat gatacgtttc ttcactttct tttgttacgt
    40681 ggttatcgat accatttacg gataagattt cgatggttgc ttcaggaaat acttcccaga
    40741 aattggtatc cactattact tttttcatct gttcacacct cttgattaga ataattgctt
    40801 gatgaataga tagtctgttt ggggatcatc tcccatataa aaaacatgtt cgctgtattt
    40861 gataaatcct tttttacgat aaaaagcgag cgcaacatcg ttgtgttccc aaacaccgag
    40921 ccaaatggct gttttttgct tttcttttgc tttttcaata gctgtatcta gtaaaaatgt
    40981 accgatacct tgatgtttaa atttttttcg tacatagatt cgctcgattt ctaaaaaatg
    41041 atcagccatt tcctcagatt gactcgttcc tgtatttagt ttcaggtaac ccgcaggctc
    41101 ttgatcgata ctcactagga aaaaatcggt ttctttatct tttagctctt ctgtaagttt
    41161 ttctagtgca taagcttgtg tcaagtaatc agctaaatgc tccggtgaat tatcttttcc
    41221 aaatgtatct gtaaatgttt cgatactaat ttgttgtagt aagtctacat ctgaaggcgt
    41281 catttttcga atttcgtatt tattcacggc ttcttccttt caatagatcc ttttattgcc
    41341 ttttttgaca aaattccaat cttcgctaac attcttgcat actttattta aaagatattc
    41401 caattgtttt gcttcttcgt cagaaagacc atttaatgcg actgcattgg agtagttgtt
    41461 ttctcgaatg atgaaaggat agatttctgc tcctttttta gtcggataga gtttcttgat
    41521 ttttttattt tctttatctt ccaaacgttc gatcatccca ttggattcta atttcttgat
    41581 tgctcgggca gtcgttgtac gatctacttt gatcatttct gctagttttt cttggatgat
    41641 tccaggattt tcgcaaatac gtactaaata aagatactgt cctcttgtta aatcgacttc
    41701 tttgaattcg atattgctaa tagaatctag cgctcgggca atcattccga tgtctcgaag
    41761 gatttcagcc atttcatccc acctttattt tgtttgtaag actagttcac ctctttttgt
    41821 tgcaaatgca actattttca ataaaaaaat caagttcttt tttaataact agtaaagtat
    41881 attcttttct tcttttacga aaaagctcaa tcacaataga tctggttttt gttttctaaa
    41941 ataagtaact gtttcttcgt tgttcgcttt tctgatagct ttggcaactc ttctatacta
    42001 aaaaatccca tgttggaagt ttcattattc tcaataaact tggcctcttc accgtggatc
    42061 gcacaagcaa aaacaagttt ataatactga aaaagctgtg gaatatcttt ttgtttattc
    42121 gtatcaaaaa ctgctcgcaa ctgagtagtt tctactgtta atcctgtttc ttcatagact
    42181 tctttacgca cattttcttc aggagacaat cctatttcag caaatccacc aggaagtgac
    42241 cattctttcg tccgtttatc ttcgattagt aggaccttgc catctttttt gatataggca
    42301 cgaacatcta cttttggtgt tggatgtcct tctccaatcg ttgtgagcgc ttttatttct
    42361 tcgaaagttt cttttcctat ttcactgatc agttccaatg aaatcgtccg gatctcctca
    42421 aaacgctctt tatcaaatac atcttttcca taatatagac cagcttgaga aagtgcgagt
    42481 agtcttttgt attgagccaa cttcttgttc attctcttcc cttctttttt agcaaatgta
    42541 tgactatgta atcaagtata gaagactggt ttaattacgg caaataaata taatgaaatc
    42601 tgcaataaat aaaagaatgt taaaaaactc gagaacaaaa gacattcgac ttctctgggg
    42661 ctgtgaataa atggcgttca aaagcttgca tctgcggtaa taagccccac caaacgaaaa
    42721 atatgacgag ctattcttgt ctggctgttc ttattaattg aggcaggaca gctttttcac
    42781 aacctcccca ctaaacggtg ttctcctacc aactcctcgg tcacaagtca caatattcat
    42841 caaacatgag aagctgtttt tttgaaattg ctccttgtga ctcggagctg aacgacaggc
    42901 tcacgacctc tcggtaaaca gcgttcaaaa gccaattttt cgatattaag ccatttcttt
    42961 gttacttatt gtcattgatt tctttttcta atttatccaa tagtggagtg taatccactt
    43021 gagtatagtc tgttacatca aattcatata gtgttcctaa ctggaatttg tcatattttc
    43081 ggtcggcgat gatctttttg aatgcttctt tcgtaatatg attggtccct ttcgttcgat
    43141 cttccaatac gtcaccatag tgataatgat ccatgatata gctcaaatga cgatctgatt
    43201 cacgatcacg tttgtaccga cgttgccata acacttcaaa gtctgctgtt aggcgaatcg
    43261 tgatcacctg atatccgtat ctatctgcta attcttggag tcgtgctttt tgcttatcac
    43321 taaaaggata ctcggacaca atcactttct ttcctgcttc catatacaaa tcaagtgctt
    43381 tgtaatagaa aggataaact ttcgtccttt cttgttccgc ccgttcttcc aaattgttaa
    43441 acccgatgga ttctgcatag tattccttga tttcatcgag cgtgatggca tacatatctg
    43501 gaaatcgttc ccgtagttta ttcataagat atgtttttcc tgtaccagga ctaccagcca
    43561 gaagaatgaa atactttttc atctcattta tttcctaagt taattggact ttccaagttc
    43621 ataaacagtc caatcttatg cttgacaatt tcttttactg cttcatttgc tggaatcaca
    43681 ttatggcgaa gagacttgtt gatttctgtt tcactaaagg tttctttagc tactttcgtc
    43741 cagttgacta aaagctccgt tccaacattg aatttgcgga tcccgttatt gatcaattca
    43801 ggataatctt cttctttcac acctgttcct ccatggataa ctagggggac atcaactact
    43861 gcatcgattt ctttcagcaa gggaatgttt acttctgttt ttgatttgaa ttgtccatga
    43921 ttcgtaccga tagcaatagc taatgcatcc acgcctgtag cttctacaaa tttcactgcg
    43981 tcttccggtc ttgtatatac cttgtcattt tcagcgacat ggattccttc ttctgtaccg
    44041 ccgattgttc caagttctcc ttctacggaa acaccgtgcg catgggcata ttctactact
    44101 gcttttgtct tcaggatatt ttctttgaaa ggcagatggg acccatcata catgacagaa
    44161 gtatatccgc tgtcgattgc ttctttgata tcatcaaagt ctcgggcgtg gtctaagtgc
    44221 aagactgcat caaccatttc ttcttctgca atagctgtca ttacattgac taacacatgc
    44281 atgccgatat atttagcggt atcgacactt gtttgaataa tgatcggcga acccatttct
    44341 tttgctgcgc ctagcatagc aggaagcatt tctaggttat gcgtattaaa agctccaact
    44401 gtaaaattca actcttctgc tgtttttgtt acttctttca atgttgtata catcttttat
    44461 tgccacctta tctttttatt tgtttccttt gatcgttact tgttttgcaa tttgtcgcat
    44521 ctcatccatt ttgcccatgt agtaatcgat cccttgatca tcaccagcac gagcagcatc
    44581 tgcacgcttc tggttataga ggctcatcag ttttttatac ccatctccga tcgtctgttt
    44641 attttccaaa ctcttttcta aatattggat ggcttgatcg ttcacataca attcagaaaa
    44701 tttaagtcca acttgttcat aaagtttcgc taatttatgc ttatcttggg tagtggaaat
    44761 ctcttgtttc aattgtgtaa tctctgattg aatcttttcg gtcgcttctg gcgatagggc
    44821 tttttcttct tttgccagaa cttttttcgg ttctttttct tttttcttaa aaaatccaaa
    44881 catcattttc cctactttca attaaaatgc tttcaaaact gggcttaaga tccaggcata
    44941 taagagtgct gcggctactg tatctacatc ggttgctgta gcattgttga atcccatcga
    45001 attaaagatc gttactagca aagcgggcaa taatgtaata aagaaaccat gagcaatacc
    45061 gccaatgatc gctcctcggc gtccgccaac tgcgttgcca aatatccctg ctgtccctcc
    45121 ggcaaagaaa ttggttagca taccaggtaa aatcatcgct aagccgaaca tgggcaaagt
    45181 aaacatggca ataatcgtac caatcgttgt cgtaatgaaa cctaaaatga cagcatttgg
    45241 gctataagga aagaaaactg gacaatctaa ggcagggatc gcatcaggaa ctaatttcat
    45301 agctatccct cgaaatgccg gcacgatttc acctaacagt agacgtacac cagctagcaa
    45361 tacatacaca cccactacga attggatggc ttgtaaaaag gcaaacatga tatagttttg
    45421 atctcctgat aattcagaag cgaatggttc gcctgcaaat aaaacagtga tgatatatag
    45481 cgggaccatc acgaccatga cagacaaata agtatcttgc agaaattcga aatgtgtcgg
    45541 caacttgata tcttcgatcg actttttatt ttcgcctttc tcaccaaaaa gcttagcaac
    45601 accggcttca aataagtacc cgatcgtaca aaaatgacct aaagcaatat catttgatcc
    45661 agtgatttta cgaatgatcg gttgcgctac tgccggcata gcaatggcga aacaagctcc
    45721 gataaaacca ccgaccaaga tcaatacgat ccctcgcaga ccggcaaaat agccaaagac
    45781 tgtcgtcatc gttgccatcc ataaaattgc ttgaccagtt aaaaaaatgt acttccattt
    45841 tgtgaaacga gccaaaataa tattgaatac aaaaatggct aagaacgtca gtgcaatatc
    45901 tctacctaaa cccaaatcat tcattgcttg accattgata gattcaatag aagggatgat
    45961 cccttgcata tgaaatcctt cagtaaagat tttcccgaaa taagttaaac ttccaacgat
    46021 gatgctcgaa ccggcactca acacttgaaa tcctaggagt gtctttaacg tgccagagat
    46081 gacttgtcct gctgatttct tctgcagtag tagacccaac atagcaatca atgcaatggt
    46141 gatcgatgcc tgagtcaaaa tattttcgat gataaaattg ataacgccca tgttttgatg
    46201 tgcaccccaa aagttagact agaaatctaa ttaaaagggg tgcttttttt gcggaaatat
    46261 acatttgttt ttaagaaaaa agtagtttca gactatttaa ataacgaagg cggctacaaa
    46321 tatcttgcac ataaatatca aataaatcgt acactggtta gacattgggt aaggatttat
    46381 aactatcatg gttgggaagg cttggttgga ggtggcaaaa gctacactac aaaatttaaa
    46441 cttgatgtta tagaatatat ggaaacaaat ggtctttcta tccaagaaac tgctaaaaaa
    46501 tttaatatcg gttcaaatag aactctaagt aaatggatag agcaatatga agaaggcggt
    46561 gcttcttcac ttgagagcca aaaaaggggc agaaaaatta gtatgaattc caagctaaac
    46621 attcctaaaa aacttaaaga tgagtctctt gaagaagaag ttattcgttt aagagcag
//
LOCUS       DAZKLA010000006        45670 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000006 DAZKLA010000000
VERSION     DAZKLA010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.006"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..245
                     /locus_tag="ACG85N_000255"
     CDS             6..245
                     /locus_tag="ACG85N_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223057.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            312..521
                     /locus_tag="ACG85N_000256"
     CDS             312..521
                     /locus_tag="ACG85N_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223058.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            581..1252
                     /locus_tag="ACG85N_000257"
     CDS             581..1252
                     /locus_tag="ACG85N_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0223059.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1305..3281
                     /locus_tag="ACG85N_000258"
     CDS             1305..3281
                     /locus_tag="ACG85N_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0223060.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3294..4049
                     /locus_tag="ACG85N_000259"
     CDS             3294..4049
                     /locus_tag="ACG85N_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0223061.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4046..4870
                     /locus_tag="ACG85N_000260"
     CDS             4046..4870
                     /locus_tag="ACG85N_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0223062.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            4880..5128
                     /locus_tag="ACG85N_000261"
     CDS             4880..5128
                     /locus_tag="ACG85N_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223063.1"
                     /translation="MKIIRILVFNLSFLAATILCCGLIFSQLSIYPRYQRLFIHFLNI
                     QCASMPLEVIKKLLINPIFMVSLVLLTGLVTWVLGKKE"
     gene            5171..7270
                     /locus_tag="ACG85N_000262"
     CDS             5171..7270
                     /locus_tag="ACG85N_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFQ0223064.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7308..9365)
                     /locus_tag="ACG85N_000263"
     CDS             complement(7308..9365)
                     /locus_tag="ACG85N_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFQ0223065.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9479..9691)
                     /locus_tag="ACG85N_000264"
     CDS             complement(9479..9691)
                     /locus_tag="ACG85N_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223066.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9878..10345
                     /locus_tag="ACG85N_000265"
     CDS             9878..10345
                     /locus_tag="ACG85N_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223067.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10342..10584
                     /locus_tag="ACG85N_000266"
     CDS             10342..10584
                     /locus_tag="ACG85N_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFQ0223068.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10602..10904
                     /locus_tag="ACG85N_000267"
     CDS             10602..10904
                     /locus_tag="ACG85N_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223069.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10976..13030
                     /locus_tag="ACG85N_000268"
     CDS             10976..13030
                     /locus_tag="ACG85N_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223070.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            13030..15642
                     /locus_tag="ACG85N_000269"
     CDS             13030..15642
                     /locus_tag="ACG85N_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFQ0223071.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            15639..18170
                     /locus_tag="ACG85N_000270"
     CDS             15639..18170
                     /locus_tag="ACG85N_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFQ0223072.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18222..18554
                     /locus_tag="ACG85N_000271"
     CDS             18222..18554
                     /locus_tag="ACG85N_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223073.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18555..19148
                     /locus_tag="ACG85N_000272"
     CDS             18555..19148
                     /locus_tag="ACG85N_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223074.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19311..21005
                     /locus_tag="ACG85N_000273"
     CDS             19311..21005
                     /locus_tag="ACG85N_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFQ0223075.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            21693..23570
                     /gene="ltrA"
                     /locus_tag="ACG85N_000274"
     CDS             21693..23570
                     /gene="ltrA"
                     /locus_tag="ACG85N_000274"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0223076.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            23701..24078
                     /locus_tag="ACG85N_000275"
                     /pseudo
     CDS             23701..24078
                     /locus_tag="ACG85N_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            24078..25283
                     /locus_tag="ACG85N_000276"
     CDS             24078..25283
                     /locus_tag="ACG85N_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0223077.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            25299..25943
                     /locus_tag="ACG85N_000277"
     CDS             25299..25943
                     /locus_tag="ACG85N_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223078.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            25954..26760
                     /locus_tag="ACG85N_000278"
     CDS             25954..26760
                     /locus_tag="ACG85N_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223079.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            26782..27012
                     /locus_tag="ACG85N_000279"
     CDS             26782..27012
                     /locus_tag="ACG85N_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223080.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            27558..27833
                     /locus_tag="ACG85N_000280"
     CDS             27558..27833
                     /locus_tag="ACG85N_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223081.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            27875..28297
                     /locus_tag="ACG85N_000281"
     CDS             27875..28297
                     /locus_tag="ACG85N_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223082.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            28316..29908
                     /gene="mobP2"
                     /locus_tag="ACG85N_000282"
     CDS             28316..29908
                     /gene="mobP2"
                     /locus_tag="ACG85N_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFQ0223083.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            29920..30255
                     /locus_tag="ACG85N_000283"
     CDS             29920..30255
                     /locus_tag="ACG85N_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223084.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            30402..30596
                     /locus_tag="ACG85N_000284"
     CDS             30402..30596
                     /locus_tag="ACG85N_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223085.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            30628..33000
                     /locus_tag="ACG85N_000285"
     CDS             30628..33000
                     /locus_tag="ACG85N_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFQ0223086.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            33061..34521
                     /locus_tag="ACG85N_000286"
     CDS             33061..34521
                     /locus_tag="ACG85N_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFQ0223087.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            34532..36736
                     /gene="topB"
                     /locus_tag="ACG85N_000287"
     CDS             34532..36736
                     /gene="topB"
                     /locus_tag="ACG85N_000287"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0223088.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            36752..36901
                     /locus_tag="ACG85N_000288"
     CDS             36752..36901
                     /locus_tag="ACG85N_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223089.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            36975..37568
                     /locus_tag="ACG85N_000289"
     CDS             36975..37568
                     /locus_tag="ACG85N_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFQ0223090.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            37584..38483
                     /locus_tag="ACG85N_000290"
     CDS             37584..38483
                     /locus_tag="ACG85N_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFQ0223091.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            38486..38971
                     /gene="ssb"
                     /locus_tag="ACG85N_000291"
     CDS             38486..38971
                     /gene="ssb"
                     /locus_tag="ACG85N_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0223092.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            39337..39597
                     /locus_tag="ACG85N_000292"
     CDS             39337..39597
                     /locus_tag="ACG85N_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223093.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            40038..40244
                     /locus_tag="ACG85N_000293"
     CDS             40038..40244
                     /locus_tag="ACG85N_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFQ0223094.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            40244..40495
                     /locus_tag="ACG85N_000294"
     CDS             40244..40495
                     /locus_tag="ACG85N_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223095.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            40510..40947
                     /locus_tag="ACG85N_000295"
     CDS             40510..40947
                     /locus_tag="ACG85N_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223096.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            40940..41647
                     /locus_tag="ACG85N_000296"
     CDS             40940..41647
                     /locus_tag="ACG85N_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223097.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            41812..42012
                     /locus_tag="ACG85N_000297"
     CDS             41812..42012
                     /locus_tag="ACG85N_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223098.1"
                     /translation="MNKIIPLLFVLFVGEILMRHAAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            42028..42207
                     /locus_tag="ACG85N_000298"
     CDS             42028..42207
                     /locus_tag="ACG85N_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223099.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            42548..42811
                     /locus_tag="ACG85N_000299"
     CDS             42548..42811
                     /locus_tag="ACG85N_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFQ0223100.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            42805..43155
                     /locus_tag="ACG85N_000300"
     CDS             42805..43155
                     /locus_tag="ACG85N_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0223101.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(43179..43793)
                     /locus_tag="ACG85N_000301"
     CDS             complement(43179..43793)
                     /locus_tag="ACG85N_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0223102.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            44107..44529
                     /locus_tag="ACG85N_000302"
     CDS             44107..44529
                     /locus_tag="ACG85N_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223103.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            44539..44742
                     /locus_tag="ACG85N_000303"
     CDS             44539..44742
                     /locus_tag="ACG85N_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223104.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            44953..45537
                     /locus_tag="ACG85N_000304"
     CDS             44953..45537
                     /locus_tag="ACG85N_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0223105.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT        16743 a         7205 c         9261 g        12461 t
ORIGIN      
        1 agtagatggc gcgaccagtt ttagaggcga tggatatgga tactttgaca aaaggaaggt
       61 atgtacagct gttagctcgt ggttattcac ctaaagaagt attcaaagcc ttatctaaag
      121 gaacagatat ggaaaaagaa agattgcgaa aagaattcga ttattggaga acccataatg
      181 gaatcaagga tttgaaacca gcacggccag taattaaacg aaagaaaaag ataacccaag
      241 actgaagtgt agagcgtatg tgatcatagg cttttttatt tttgcaaaaa acaaggaagg
      301 taggaaaact catgaattat gaagaaatcg aaaacagaaa aaaagtgtcg aaagaaatgg
      361 aagaaaagtt actaaaaacg atgaagcaaa aacaccttaa aagattatct gtcgcgcaat
      421 atatcaatga tatgcagtta acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa
      481 actttgaaca gttgagacga acttatagaa ttcattttta aaactttttc tatggaaatg
      541 agaaaaagtt tttttgtttt gtctgaaagg aggcagatgg atgaaaaaat ggttatttgg
      601 ttttttagga gtcactttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa
      661 acatgaagga gaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
      721 tgcacatgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
      781 gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc gtcaataaac aagtatttgc
      841 acaaattcag attcctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata
      901 cactttgagt cttggagcgg ctacttattt ttatgaagat gccgaaatgg gcaaaggaaa
      961 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atattcaaaa
     1021 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
     1081 aacgcgtaaa tttatcgtac ctgggtactt taaattaatc gatggcgttc ctgaagagaa
     1141 ctctttcttg tctttgccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
     1201 ttatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttt
     1261 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1321 atgtaaaaaa agctggaatg cttgttggaa tgttaggaat cattgcaggc tgctttcagg
     1381 ggtatcaagt acatgcagaa gatgtcacgc aaaaaacacc accagagaag gtcaatatca
     1441 cggttcataa attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca
     1501 aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1561 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgagagaag
     1621 attctgatct aaccaacgcg aaagtggacg agattgtaca agatgtagaa gacaagggat
     1681 ctaacagtga gtacgtcaag aatgcaacca acaaaattac ttcagctgta gatgaaaatg
     1741 gtgagatcac ttttactgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     1801 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     1861 ttgcgccaat gacagataaa acaggatcag gatttttaaa aaatattcat ttgtatccta
     1921 agaacattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcgcagacc
     1981 aaagcaaaca aaccctttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     2041 aaggaacaaa attgggtgat cttgtatcgg atgatcaagg aaagctgact gcgactgatt
     2101 tgacgttagg aaaatactat tttgtggaag ttccttcgga agttgtagtg ggatcagatc
     2161 aagaaccaac agcagatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata
     2221 aattaacctt tgaaattacg aatgatggcg tgacaagtga cttaaaagct tcatacgtaa
     2281 actataaagc accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt
     2341 tccaaattgg tgacgcagta aattatcaag gaacgattca tattccaaca gacattgcag
     2401 gtggcgcaga cggtatcaca gtaaacggtg tgaaaagcga aacttctcct tacagtgtat
     2461 ttaaatgggg agatacagca ggtaaaggac tttcttatgt tgctgaaaaa gcagatatta
     2521 aagtaacaaa taatgatggt tcagtcgtat taaaagaaaa tactgactat aaaatcgaaa
     2581 attctgatag tggttttgtg atcgatttta tcgtaaataa tggacaagta agtgatacag
     2641 ttgcgaattt acatggtcaa gatctaaaaa tgacttataa catgtttgtg aatgatagtg
     2701 ctgaaatcgc aaatccatta acaaatagcg tggatttcgt ttataacaat aatccattta
     2761 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggc gcaaaattct
     2821 taaaagtcga tagcggccta tttggaacag gtatcaaagc aacaccgctt gaaggcgcag
     2881 aatttgcagc taagaatgca gaaggtaagt actacggtgg acttactgat acagataaag
     2941 atggtgtcaa agaagcagtt tgggtagacg atgtagcaaa tgcagcaatc ctaaaatcag
     3001 ataaagaggg tcattttgaa atcactggtt taactgaagg agattactca ctggaagaaa
     3061 caaaagctcc tgaaaactat caaaaattaa caaaagagat ctctttcaaa gtagacaaag
     3121 attcatacaa agaagaaaac cgaatcacga tcaaaaataa tcaaaaagct tccgtaccaa
     3181 tgactggtag caacggtttc caatcttacg tattgatctc ttgcttgttg ctaggagcag
     3241 ggggactttc tgcagttgtt tactttaaga agaaagccta gaaggatcag ctaatgatta
     3301 ttcgtaagcc cccaaaaaga agagtcatgc acaaactgat gatatttttt tggttgctgt
     3361 cggcagtagc agtctattta attccagttg tggaaacaaa tgtagcgaaa tttcaacaac
     3421 agcaaagaat tttagccatt gaaaatcttg aaaaaacaga ttcttctttg gatgttgcga
     3481 aaatagagtt aggcgatcct gttggtgttc ttacaattcc aagtatttct ttaaaactac
     3541 caatttatga cggaacttcc gacaaaattt tagagaatgg tgtagggatt acggaaggaa
     3601 ctggcgatat tacaggagga aacggtaaaa atcctttaat cgcggggcac agtggacttt
     3661 ataaagataa tcttttcgat gatttgcctt cagtaaaaaa agggcaaaaa ttctttatta
     3721 aggtaaatga agaacaacat gcctatcaaa tttatcgaat tgaagaagtt aaagcagaag
     3781 aattacaaaa aaattattta gattatctta tgcctgaaaa agaccaagat aggatcacgt
     3841 taatgacatg cacaccaaaa ggtgtcaact cacaccgctt tttagtgtat ggtgaacgag
     3901 tttccttcag taaagaagat ttaaaactac agaaaaaagc aaaaaatagg aaatcaattt
     3961 cattggattg gcgattagca ctgctattcg cttttttttg cgttcctatt atttttattt
     4021 ataaaaaaat aaggaggaga tcggaatgaa taagagaaaa tggctgtttg tttgtgtagc
     4081 agctctcttt tctactgtag cttattcgca actgtctttc gctgacagca gtgatagtag
     4141 taccactcaa acaatagata attcaattac tgcaccaagc gaaacggaca gttcggagaa
     4201 tactacagaa gaaacggcag attcaaatat aagctttact gaatcaacga atccaacgtc
     4261 agaaagttct tcagagactt cagaaaagcc gcaagacgta gaagatacgc aagtgatcaa
     4321 gcaagccatt catattcaaa aaattatttc ttcgaatcaa atcaacaatc aaggcagtca
     4381 agtagcccag caagaaggta tcaatggcgc gaagtttatt gtctacgata tcacgaatat
     4441 cttagaagaa atgtcacaag atgataatga gaaaaatcaa tctgtagaaa taattgagtc
     4501 aaaattgaaa gatcgagcaa aaaaactttc atacgaccag ttgaaaaaag tggctgaagg
     4561 agaaaccaaa acgattgatg atcaggaagg cgtgattgaa ttttccattg aagtacctgc
     4621 aaacaaaaaa caagcatact atatcgttaa tgaatcaagc cctgaaaaca tttcaaacag
     4681 tgaagatatt gttttattga cacctgtttc aaatgaaaaa ggtgaatttt tgacggatgt
     4741 ttggttatac cctaaatcag agaaatcaga gccgaaagaa gaaattaaaa aagttgtgtc
     4801 aactggtgta aagaagaact tttttgaaaa ttgttgggaa ttttttgctc aattattttt
     4861 tgataattag gatgaaatga tgaaaataat aagaatatta gtttttaatc tatccttttt
     4921 agccgcaaca atattgtgtt gcggtttaat tttttctcaa ttatcaattt atccaagata
     4981 tcaacgattg ttcattcatt ttctaaacat tcaatgtgct tccatgccat tagaggtgat
     5041 taaaaagctc ttgatcaatc caatttttat ggttagtttg gttcttctta caggattggt
     5101 aacgtgggta ttaggaaaaa aagaatgaaa agcttacgat cgtttaaaaa aatattagat
     5161 ggagggcatt atggaaaata aaattcattg cactacggga agagtaatta ctttagtgtt
     5221 gtttttgttc cttcttcttt ttgcgagggg aactgcagtc caggccttag aaaaagttga
     5281 tccgagtcaa atcaaagaaa ataataatct ggtgctttat catgatagtg attcattgag
     5341 aaatatcgaa gtaactggga tcaatggatt taagatacta gatgagtatt caactaaaaa
     5401 acaaaaagta cttgcttggc caaaagtcaa agatttgaaa agtacgtcaa aaatcgttgc
     5461 ccattatcaa aaagtaggta cttatctagg aaaagaaatt gattgtgacg tgactttttc
     5521 taattttgta gcgcacgctg gggttaacca agacggatta gctaaaacaa ttaatggcaa
     5581 aaagtattat aattatttgc tctttgataa aaatatttat cgaggatttt tcatgcagtc
     5641 aatcaatgtc atgaatgcaa aactagaatt taagtatgca ggaacaaaaa caaaagtagc
     5701 tttgagtgaa ccacagccag aaattcaatc aaaaatgaga tcgatgagag cagctggtat
     5761 tgatttaaac gatacatttc tatccattta cagtttaaat ggacagtatc agcaacgtgt
     5821 gtcttggtat aatgacaata atgaatctgt cggtgaacgt aatattgata tgagagaatt
     5881 tgttgggtat gaaaaaatgg gtagcttacc ttattttgtc acaacagata cagcatgtgc
     5941 agaatacaaa gctcctgcgt tatcaacaaa caatttaact tcaaaagtag tgggaggacg
     6001 tgcagaaaag gcttatagct cgaatgatca tttcaccgat gttgtaggag ctgatactta
     6061 tcacagaagt ggtgtaacgt atacgcttca aggcgcttcc ccaacattca tgattggcgc
     6121 aaatacgaat agtatgatgt ttagctttga tactgcattg ctatggacac cacaaccatc
     6181 gaagcctaca aaagaagtgt ttaacaaagc taatactgaa gaggcagcac acaatattga
     6241 caaaaaagtg attccacaag gatcagatgt ttactatcat attcaccaaa agtttgatgc
     6301 attaacagtc aacacaatga acaaatacaa atcatttaaa atcactgata cctttgacag
     6361 caaaaatttt gatatggtat cggatgggaa aaactatgat ggcgcattgt ggtatgttga
     6421 agcgaatggt acttggaaaa aagctgatac aggaggaaag ttaactgtat caggtaatac
     6481 agtcacttat actgcttctg acagcttttt agctaacgct gcaaattacg gtagggatta
     6541 tattttgcag ctccatatga gagcaaaaaa tgatgcaaaa ggaattgcac caaatgtggc
     6601 tactgtaacg tttaacaatg atccgaacaa aggaacacaa aaaacaaata cagtagaaaa
     6661 ctatttctct cttaaaccaa ataaaaaagt ggaacaagat gggaaagatg ttgatggtcg
     6721 aaatgatggt aaaaaaggaa cgcctacagc tcctttaaat gcaggatctg aagtacaata
     6781 tctagtcaca caaaaatggc atacaaaagg tgtagacatt gcctcagatc attacaagca
     6841 atttagtatt caagatccag tggaagatcg tttgacgtat aaagaaggtt ctgcgcgagt
     6901 gatagatatg agtacaggaa aagatattac ttcagaaggt acactaacct atgatagcaa
     6961 ttcaagaacg ctgaagtggg aagcttccgc tgatttctta agtaaaaatc ttttagatgg
     7021 acgagaaatt cagctgatat ttacagctaa aactcctttg aatgaagaat tggatatcga
     7081 caaccaagca aaagtttcag tagaaagtat tgagaaagca actaatgtag taacgattgg
     7141 ggtaaaacct aacttacctc agatcattgt gccaaaaact ggatcgaatc atttagtgct
     7201 aactgttatt ggaagtatat tatttcttac tttagcatca ttgggctatg tagttttaaa
     7261 actcaaataa atataaaaac aaggctgaga atcagccttg ttttctttta tttgaagatt
     7321 ggattcgcac taataaatac attatctacg ccaattctac cagcagagtc aactacaaaa
     7381 ttatgaccta atccgagaat actttggtta ttataaagta aatttgcatt atgggcaggg
     7441 ctatttttcc atcctaccat ggcatcgata gcaccatcgt ctgataaaag gtcatcaata
     7501 tacagcccag tttgacttat gttttctccc ttagcaccgt agcagcctaa gctttccaat
     7561 aaatcttgga aagtaccacc accagggcga tagtgactga agctgacttg taattcttta
     7621 gctcgaatat ctgcagcctg ttgaacaata tcattgaatt gcattggggc aacaccattt
     7681 tgttggcgat aatcatttaa ccaagcaagt tctttttgac gcataaagtt acgtaattcg
     7741 tctaatgaaa tctttttata tttgaatgtg attgttttat tttgatcgac gttattaatg
     7801 gatcgttggc cgtttttatc cacggtaacg gtaggatcgt catttctgca caagaaataa
     7861 ttttgatcag tcatgttttt ccaaggagta gcaaagctat ccccttcgta tactgttttt
     7921 gattcactag agagaacagc gttggtatca gcatttacat gattaacagt cacattaaat
     7981 ttacgagtgt agttgaaaat ataattttga ctttcagtca cattgtttaa ggttactgaa
     8041 tttgttggtt ctacccgatc gtctaaaaca tatccttgaa tagaaggagc agttaaagtg
     8101 acactgtcac cgtgagtgac attgactatc tgttctttca aaatattaac tcgtccttcg
     8161 tttttgtagt tgttattgca caacaaattg atttgatgca cttgattata attgaatgtg
     8221 attgttgtat tacctgtgat tgttttgctt tgagtatcat taccaatcac tttgtagcta
     8281 aaattatctt tagcgccttc gctatattta aatgcttttg ccttagcagt gtaagtagaa
     8341 ccagaagtta cttttgcttg atctgtagca atcacttgac ctgtttgatt gtcaatatgg
     8401 ttaatagtaa ccgtatattc ttttggtgtt tcaggtttgt gttgcaaccc tttaaatgca
     8461 gcatcaagac tatttttagc attgtttact tgtgtttgag aaaccttaga atcgtctagt
     8521 acttttttag cattggataa agcagtctga aaattattcc aagattcttc tgtaaaatct
     8581 cctttagcag ttgattttac tcggttgtat aaagcttgta attcagcttt attcacagta
     8641 ggaactggtt ttttctttaa tcctttgtaa gcagaatcta atttattttt ggcactatcc
     8701 acttgctttt gagtcgcttt tgagtcattt aacactttct tagcattgtt taaagcggtt
     8761 tggaatgcgt tccaggtatt gtcggtatag tcaccttttt tcgttccctt aacgctcgta
     8821 taaagagctt ctagagccga tttattcact ttgggtttag aggtgtcagc gccataccaa
     8881 ccgatacctt cgtatttcca gccatagtct ttggttaacc attgtacttc tttccaactt
     8941 gcagtgtagt gatgtgagcc agattttaca ccaggaagga aaaggcggta gatcggttta
     9001 ttattttttt cggcagaacg ccaggcaggc ccttcataag tccagccgta atttttagtc
     9061 aaccatttca cttcatccca actagctgtg taatggtgat cacgaagacc tttattgtac
     9121 aaacgataga caagatggcc accgctagtc ggtgcttccc aggcggggcc ttcataattt
     9181 ccccatttta tctttactag atggtcacgt tcttttttgc tagaggtgta aaaatgttca
     9241 ttattatttg gattgtaaag gcgatacatg acgttagttg aagcagcgtc aacagatcca
     9301 gcaagactaa gtgaggcagc aaatgttcca atagctaaaa cagtttttgg attaaataat
     9361 ttcatttttt caactcctaa atttaattaa aaatgtttac atgattatta tatatcggct
     9421 aaccgatata gtcaataaaa aataagaaaa gccttatata attttggctt ttcttatttt
     9481 atttgttagt tgaagtatta atatcaacga tttcttcagc caatttattt aatttcatag
     9541 catttatgaa tctcatgttg tctatactag agcgtttgtt ggcaaggtca cttactgtag
     9601 ttaagggtac gccagattct atggatatag aacgaactga tttaccacta tcaaacagca
     9661 accattcgat tttcttttta tcagcgtaca tttcaatgct ccttcttgtt ttttctttat
     9721 tatagctctt ttttgtaatg aatcacaata tcaagagaaa aattttctat agaaaggtgg
     9781 atttttatga gtattcgttc tccgtgtatc caaatgaggt ttgttgctac tttcgttatt
     9841 tgatatacta aaaataaaca acgaaagaag gaataaaatg aagaaaaagc aactatggtt
     9901 tgtctctatg tttgcaatat tttttattat tttaagtgga tgttcaagta ataagaaatc
     9961 ggaagctaaa gaagataata gtttattgca accaggaact gtttggaaag aagaagtagg
    10021 tggtcttatt cataatttaa aaattataga tgatactacc tgggaatatt ctgaaagtgt
    10081 atggcatcca gatcctgtac aaataacagt taagaggcaa aaagactatg aaggtttaga
    10141 aagatacaaa atagtagatt ctgcaggcat tagggaattt ataaacaaac ctgattcatt
    10201 atttattgtt gttccctatg agaaggatgg cgtaaaaaaa ataatttttc ttggatctag
    10261 caaggatgag aagcgaacga aagagaaatt aattcatgat ggatcagagt taaataaata
    10321 caaattacaa aagactagtg agtgattgct agtcttttta tttatcctga aggaggaatc
    10381 acagtggcaa aagttaaaac accattcgaa aaagccgcgt ctaaaatctt agaacgccgt
    10441 ggtgtttctg aaatacagtg gaaaaaagaa gttcttgaaa aagcacaatt tgacctatta
    10501 cgaggaaaag ataaggactt agaagaatat atctatcaac gtgaacgtga aaaacttgta
    10561 ttcgatgaaa ttattaaaca ttaaaaggag cgaataatct tatgtttgaa tcaattacaa
    10621 tgaatttttt aactcaaccg aaagtagtag ctgcagttga tatttttaga gcattacaaa
    10681 ctgccgcaac tagtttgaaa aataatctga tgatttttgc ggctgtagct gccgttgcct
    10741 ccattgctct tggaggtatt ttcttcttct ttgggcgaag aggagctgaa aacgggaaga
    10801 cacacgtagg ttatgtattg ttgggggtag gagttatttt agctgccgct tctattgtag
    10861 tttcaatgtt ctcaatgttt ggacaaaccc cacaaggact ataaaaaaaa caagtaggag
    10921 gagtaaaaag tacgtggcta ctttttactc ctttttttat gaaaggagaa tattcatgtt
    10981 ttttacgatt tcaggaaatg caaaattgga taaagagtta ataagaaaag caacgaagaa
    11041 agtgggaaag cgtacttcct ttttaccgaa aacaattaaa tattctgtca ttgaaacgga
    11101 agaacagcta catgtgttag gacaagtgtt agaacaagag aatcaatctt ctgttaaatt
    11161 gactctcacg ttacatgata aagaaggaaa aatagaaaag gctggcacag aatacgtttt
    11221 tggcccactg gattatcaaa tttcagaagg tttttatcct acattgatag atttaatccg
    11281 ccttgatgtg tttaaagata cggatgaaac tatgagtgtg gaagagtctt atcgaaacga
    11341 aaaactgcta caagttttag aagaagagtt agagagcgac caaagaaaag tgaatcaagt
    11401 gagtgaagtc gaagtaaatg aagaagtacc atgggaacga ccaatctttc caaagaagga
    11461 gtcaaaagaa aaagagacag tctatgttga cgaacaagct gatattccga ctcagaaatc
    11521 gatatttgag gaagagaacg atgtgatggg tcaattggag gtagatccct ttgaagaaac
    11581 aatggaggat gaagagacgg acgaagtaga agttgatcca gttgaagcta atgaagagtc
    11641 aacatggact gaaggggatt ttcaggaata tattgaacca cttatcagtg aagaagtaga
    11701 aaaaaacgaa acagagatac aaaaggcaac tgtatttgat tatattttgg atcaatatcc
    11761 gccagagcat gaatggatga aagaaaaaaa caaattattt ttaaatgagc tttattcgaa
    11821 atatcaatta ccccaaacct atcaagcatt tttagagagt aaagaagaat taatctcaca
    11881 gggtaaaggc acacttttag aacagttaga gtctgtccaa tcaacaaatt ggcatgagct
    11941 tgcacaagaa gatttattag ccacctttga agaacgtcaa caagaaacag atcaagaaat
    12001 taatgagtat gcgacggaac agtcgtctaa ttgggagaaa gtaaaagagg aactaaataa
    12061 agaagaacag cgagtgattg aagaagaagt acaaaaagtc aaagagcggt acgaaaagaa
    12121 acgcgacaat gcttattcct tgtgtgtaga aaagatcaac aattttacta agacgaatca
    12181 agaagcgtta tcgaaagaaa aagaaaactt gttaaaagaa cgtattaaag aacgaaaaga
    12241 agagttctac cgcggtttac gtacagataa gctaaagatt agcaaagaat taaatgaaca
    12301 actgaataag ctatatcaaa caacgactga agttcttatg gaaaaacacg aacagatcca
    12361 gcaagaatta caagtacaaa ttccaaaatg gaaaaaagag tatgaaattc aacaacaaga
    12421 attgaaagaa caagaagaaa aacaacggca aaaagaaaaa gagcgtgaac aattagaaat
    12481 aagaaaacgg gaacaggatc ttgcggaaca aaaaatggcg atagaaaaac aacggttaga
    12541 gcaagaaaag gaaaaagaag cagctgctcg taaggaacgt caagaacaaa tggatacatt
    12601 gcgtcaagca cagttgaatt atctagcaca acctcaacca gtagctgctg ttcaaacgcc
    12661 agtgaacaac caagcaaatt taactccttc accccagcgt tcctttaacg gatggatggt
    12721 gggatgtatt gcaagtttaa gcttgcttct aggtggagga acagtatttg cttttaatca
    12781 tatcaatcaa tcttcacagg ctgattctgt agccacaacg gcgtcaatca aggaagaagc
    12841 ccaggaacaa gcaagaaaag agtatgaaca acaattagaa gaattattga atcgtaccaa
    12901 tcagtcaaca acagagtcaa gcatggaaaa tagcaaagag agtgatccta gtaaggctaa
    12961 agaaggatca agcgattcaa ctgtacactc aaacacttcc gaaagttcag aatcaaatga
    13021 ggtgaaataa tgcgtattag agaaacaacg agacgaaaag acaaacgatt caatttagaa
    13081 aagaataggt atctttctca tttctatcgc tatactcctt caggagaaga gctaccagaa
    13141 aaacatgaca aacgtatttc tcaaaagatt ttttatagtg gcttagctta tcttccttta
    13201 aactatgttg ttacaagaat accagaagca tttcatcgat ttgagattat tccaggaagt
    13261 ttacaaggag tgaatatttt tcagttacaa gaaatgccgc agttattcca ttttgattca
    13321 actggttttt ggaatccttt gaccccaaca acaagtttag gcacacgttt agttgtttct
    13381 acgatttgtg ttgttccagc tgtacttatg ggattgaaaa tggacatgtg gtttcgtccg
    13441 ctagcagatg gacaaaaagg ggataatcga ttaatgacct taaaagaaat ccaacagatt
    13501 tatccgcaaa tacctgaaac aaaaactact tttccaggtt atggaggtat tccaatcaca
    13561 cattacaaaa aatattggta cattcaaaca gatactgtta atacgtgtat tgttggtacc
    13621 tctcgatcag ggaaaggtca gattgaagtg ttagccacca ttgacaattt atctcgtgca
    13681 gaaaaacaaa gttcaatggt cgttaatgat ccaaaaacag aattatatgt tggtagtaaa
    13741 gatgaactag aagctagagg gtatgatgta tatgccttta atatcaccga tccactgcaa
    13801 tcaatgagtg ataatccatt ggcactcatc gtgaaatact ggaaacgagg agacatagat
    13861 actgcaacac agttaaccaa tactttcaca aatacgattt attatgatgc caacgcttca
    13921 gataacaaat actttaacga aaacgcgcag aaagctgtca atgcgcttat ttttgcgttg
    13981 ttagaacaag ctgatcaaac aggagattac tccaaattga caccgaataa tctcgttgag
    14041 cttctttctg agattggcgg ttttaattat tatccagatc cagacaatga attcaaacag
    14101 ttgaatgcct tagatgaatt tatgaatcag ttgccacctg gaaacattgc aaaaaaacaa
    14161 tatggggcaa cgaaaattgg atctgataaa gcgaagggaa atattttatc aacggcaatc
    14221 acgggattga atccatttac tctaacgaaa attgcaaaga tgacttctca atcaacgatt
    14281 gattataaat cggttgggtt tccaaaatac ttagatttga aattacatga aagtctttta
    14341 aatcaacgag tcgtagtaga attttatcat aagggaaaga aatttcatga agaacgtgtc
    14401 aaagtaggct ataaaggatt tagtgaaatc aattttgatt gtcatttagt tgcaggtgat
    14461 caagtttgtt taaaatacga tcatcaagga aaagaatatg tggcttggta tgaattttct
    14521 caaagagtat tgaaagacga agagggtaat atcgtttatc ggaaaaaacg aggagaaacc
    14581 catttgccag aattagaaaa agaagccgtt ctaacattag atgaggaaaa aagtaactta
    14641 ttagtagagt ctattcggat gcactatacc gataaaccaa tggctatttt tatgcttaca
    14701 cctgattatg atccatctaa ccatatcatt gttagtattt ttcttagtca gttatataaa
    14761 gaattatcta cgcaatgtgt taagactaaa ggagataaat gtcaccgcag gattcatttt
    14821 attttagatg aatttgggaa tatgccacca atggataata tggaaggaat catgacggtc
    14881 acagctggcc gaaacatgtt gtgggatctg tttattcaat cttatcagca actttatgcg
    14941 aagtatggag aacaaaacgc agcgattatc aaagaaaatt gtcagacaca tatttatatt
    15001 atgtcgacag atgataatac catcgaagaa ttttctaaaa aagtggggaa taagacggtt
    15061 gaacaagaga acagtaccat caattcatta ggtatgaata ctcatatcaa tcgcaatgtt
    15121 gattcagatc gtatcttaac tcctgaaaga atttctactt ttttagaagg agaaacgatt
    15181 gtactcgctc ctttaaaacg acaagatttg aaaggtcata aaatacggcc gtatcctatt
    15241 tttaatcatg gggaaacgaa tcttccttat gcttatcaat atattgaaga tttcaatcca
    15301 tcgtgggata tcaatgatat agacatacat tgtacacatg cggacttgga tcttaaggct
    15361 ttacaaattg attatgatta tttcctacca gatgaatatg ctagaaatct ctatcatgga
    15421 ctgcatgatc aattagaaga aaaaaacgaa gaaaatatgt tttataaaat cgttcaatct
    15481 tttgagaatg agacggctaa aaaattgtta attcaatttt acgaagaaca agcagcagat
    15541 cgacttcaag aatatgttga aaagttgatc aaaaataaag aaatttctga aatgattggg
    15601 aaacggttgt tattggaatc tgaaaaggtg gtgaaagaat gatcattgga ggaatttttt
    15661 ctggcatagc cgactggtgg tcaggcttat tcggagaagg ggatgcaaac cccaaaaccg
    15721 taacgaaagt cttacaagat tatgggaatt atctgcagta tcaagatgta tttagttgga
    15781 tttggaacac ttttctgtgg ggattgatta aaggattata taatctcaat actttactag
    15841 aaaaaaccat ttatcaaagc tttgatttaa aagacttatt gaacgccgct ggggtaaatg
    15901 cactatttca aagtttgatt agtaaggtat tggctctttt ttgtgtcgtc acattaattt
    15961 atttaggctt gaaatttttt atgagtaaac acccaccaaa agtcaaaaat atcttggtta
    16021 atttatttat ggccatgatc ttgatttttg gtggtagttc tttaatcgat gaagggttaa
    16081 atatcagtaa aagcttttac ggagacgtca ctgcagcaaa taaagcagat aaaacgggat
    16141 cacctgcttt tcaaattatt caaaataatg tgatcgatgt cagtacgtta ctagcaactg
    16201 acccgaataa agtagaatcc ctaccaacag ataaaagaaa tgctttaaca gcgaaaaatt
    16261 ttaaaacagc gaatatcaat gggattatta ctcctgaaca agctaaaggt atggcaggta
    16321 atgataaaag tgggttatca gatatacaaa aggatcggtt aaaggcatta caataccgat
    16381 tggaactaga tgataatggg aaagaaattc ctgttcaaat tagtgacgaa ggcttagcaa
    16441 aatatgtcta tacttcaggg tatcgtcgat acagtagtaa accaggtatt attttagctg
    16501 gtgaatcttc tttggcagta gcctatttat ttatcctatt tacgatcgtt acttgcatta
    16561 ttgagctggt atttaaaaaa ttctatttag tgattgccgc gtcaacggac tttgagacag
    16621 gtcaacggat gaaaagtgcg atagaagata tttctcaatc cttcttgtta ttggcgttta
    16681 cgattttaga attacgaatc tataccttaa tgctttcagg tatcggtgat ttgcatgcgg
    16741 caggaaagat caatggattc ttgtatgtcg ttgctttgat tgtactaacc attgcgctat
    16801 tcaaaggctc tcaatcagtt acgaaaattt tcggcgttga tacaagcctg aaaaacggca
    16861 gtaattctct tcttagcatg tttgccttag gaaatattgc gaaaaatgtg ggtagcggag
    16921 ctaaaaacct tgcaagtgct ggtaagagtg gtttaagcca tttaaatgat attcgtaaaa
    16981 atggatttgg ccatcgaaaa gatgttaccc ctgataacgg ggaagaacaa tcagacgata
    17041 aagctacaac tgtacctaat agtaagaaaa cagtttccga aatgttcggg aaagcgaaaa
    17101 atggtgtttc taaagccgca gaaggtcttg gttacgtcaa tgaacgtggg ttaaaaggaa
    17161 cagctgaaga tcttacagaa aaagccaaag acaccttaga agataaatta gacggcacga
    17221 gagctaaaga attggctgaa gctgtgaaga atccatccga agcactagaa aaacaaaaag
    17281 aaaaagcttc agaagtcaaa gaaggactaa aagatgcgta tggtgaagga caagtcaatg
    17341 cagcagtgaa agctaataca acaaccgaaa aagaaaatca agaaggtata gtgattcctt
    17401 cagtcaaaga tcaccaagaa gcgttgcact ctaatgaaaa ttcgattgaa gagaaaaatt
    17461 ccatagaaga aagaaattca gaagaaggaa aaaatggaca gttaccaaaa actgatccag
    17521 aacaatatcg ctataaacct aacagtgacc ttgaaaatga gaagcgaatt tcagatttat
    17581 caaaagtaaa tcaagaagac aaagcagaaa atgagggaca gttaccaaaa actgatccag
    17641 aacaatatcg gtatagatct gttaactccg aaaatgaaaa acgcatttca gatttaccaa
    17701 aacgaaatca agaagtacaa acggatgagg attccatcgt tctgccaaat gataatggaa
    17761 tcgaagttgg aactgaaaaa tcaacgaaaa ttggtttacc taaggcacaa aatttaccaa
    17821 tgaaagatag tcgtttgaat caattcaagg ataatgatca aagttttgtt catcaatcac
    17881 ctgcaggtac tgttcaaatg aaaccaagtc gtttgcctga aaatggaaat ctgacaactg
    17941 tttcttacaa tttatctgaa agtggtgaga cagggaatgc gtccgttcaa aatgttgtga
    18001 caagagaagc tgcaccaaaa cctattcaaa acgttcatac aggttacaag ccagcaaatg
    18061 tggcatttac tgcaccaaca aaaataaatg tgacaagaga aacaaaaaac aggaacaatg
    18121 cattgaaaga acaactattg agggatatca aacaggatat caaaccataa aaggcctaca
    18181 agttaggcct tttttctatg tatgaaaagg agaaatcatt tatggataaa tatagtaaca
    18241 tgaaaaaccc tccaagattt agtggagaat ggaagttttt cgcttttttc tttttagatc
    18301 tattaacgct tggtggctgc tttttttggg cgaaaatggt caataatctt gtccacttac
    18361 cactcttttt tgcactcttg aattatggct tatggattgc gatagcgtta gtgattgttt
    18421 ggcgacccta taataaccca ggtcaacgac aaatttttgc gattatcgag tctatttttt
    18481 ttgcggacaa gaaccattat catgcgttag atcctaaccg aaattatcat ggtacgtata
    18541 aggaggatga ataaatgcga gtcaaaggac aaaattcagt tgaaaggaaa tccattttgc
    18601 agtttggaaa accagaagag cgctctatct tggacttatt tccatataaa tcctgtacag
    18661 aagagggagt cttaattact agagaagatc attttcaacg ttattatcgt gtagtttcta
    18721 cagacgtgga aggattaaat gaacaagaga aactagaacg aatgaatcag ttgaccaccg
    18781 tcatgcgtac gtatgtgcca acaattaagt tagttagttt gaccacagag acggatctaa
    18841 cggaacaaat cgtgcaaaag cggcaattat tagataaaaa tcgtatggaa cagtccttag
    18901 gaagaaatct acaacgatta aaaaaatatg aaaagaaatt agtcgaagaa atccaggaat
    18961 tgaaacgagc agaacaagaa cgtcctgact taagcttctt tttcgtcatt gaaggaaaga
    19021 caatcaagga tttacaaacc agaaaccgtc agttaatgcg atctagtggc attttagggt
    19081 taaaaccact ctacaaaaaa gaattgatca agattctgta tcgaatgaac aatatgaatg
    19141 atgagtagga atggagaaga gagaatgaaa tatccgttga tatggttaga aaaagatgtt
    19201 ccgaaggaag agaccactag aaaatttcct cacactttaa gcctgggtaa gttgggaaaa
    19261 gggcaatctt ttaaccaagt aaagaatcaa acaagaagag gtgactgtac atgttagaag
    19321 caattaaaac agctttttca ccacaaaatg atcaaaaagt acttgatcca ttaaagcaac
    19381 taggaaatcg ggaaatagaa attgtaaaat ccaaagggta tgatttagat tttttaaaac
    19441 aagtgatgcc acaaggtggg ttaaattttg atgaagaata tgtgacgtat ggtaatggtt
    19501 attcacgatg tgtgacagtt tatcactaca caaaaaatcc tacgcttttt tggttggcca
    19561 atctcatgaa caatcctcat acagtggcca cactcgatgt tagcacagat gaaaagcaaa
    19621 aagtgattga tcaaatcaat cgtgccttag atgaattaaa tgatcgatca gataatgaac
    19681 gaaaagcgac tgaccgaaat aaaagcgcgg aagaatacct ggaactagcg gaatatgcta
    19741 gaaaattgac ttctaatggg gaaatttcta agcaagtcaa agtgagagta catgtttacg
    19801 gaaacacgat tcaagaagta gatgaacgag cgcaggaact gatcgaaaat ctcaaaggga
    19861 tggattatcg tgcggtgact tatctgcata aaaccaaacg agattggcaa tcgatgttta
    19921 ccatgttcga tgaacaagct gaatggttcg gaaatatcaa agggcaatca ctaccatcgt
    19981 taaacattgg cggaggtttc ccgttccacc accaaacgct aaaagatcca ggagggacac
    20041 aatatgggga aaccatgaca ggcggcccat ttgtctggaa tgcgacgtat aaaaatagtg
    20101 tacggttgtc tactaatacc ttgcttctag gaatgatggg agctggtaaa tcgaccgttc
    20161 ttaaaatgat cgcggaagcc catttagctg caggagactt tatttggggc tttgaaaagg
    20221 gaaaagactt tattcctttc ttaaaggaat ataacggtat tatggtgcgt ttagacggat
    20281 ctgacggaat gatcaatcct ttagagatat ttgccacacg gacgtatgat gaagcatcca
    20341 gtttatatga cgatggatca gtcaaagatt tgaagatcaa tgaagcagcg tcctatcaaa
    20401 cacacttaga caaagtggtc tatcaggtac agttagtgag tcctcagcta aaaggaacga
    20461 tgaaagcaga atttaagacc tacttaaatc gtttttatga agagtatgga acagtaccac
    20521 gcggctttac ttcctcaaat tcgcgttcaa atactgatac gcaagtcaca gggaaagatc
    20581 cagaagctta tccgacgttc aaagaatttt tagacttttt aggacaactg gaattaccag
    20641 gtgcttccca agagaaaaag aatcgaaaag aagaaatgga atccatcgta gaatccttat
    20701 gtgagacgta tggcatgatt tttgatggtc atagtacgat tcgacacctt gaccaacaac
    20761 agttggtctt ttttgacatt gactctatta caggcttaga tcgaggggta caacaatgtc
    20821 aaatgtacat ggctatgacg ttgatttgga atcaggcatt gattcaagga agaagacaac
    20881 gacgtttaat taaagaaaaa caaatggaag aacaagactt caaacgcttc ttggcgatga
    20941 ttgatgagtg ccataatggt gcgacctgtt ctgcagtgaa aagctgtggg ttgcaagtag
    21001 tctaaaaact attgtaataa ctcttataac gagaagtcaa atgagtgggt aacgccacaa
    21061 agggcttcca ctgatactcc gacatgcaac aagtagttta tgcagaccaa cgttgtgtga
    21121 agctcggtga agtcggctga aggataccgt aaggtaaaag aaatagtgaa taccacgaaa
    21181 gttctctaga ggtaaagggt gtatccgata agtcgggtgt ccaaaaaaac ttaatatggt
    21241 gagtatgtac aaacgtactg acgaactagc gaatgtactg tccgaaaggc atagctccta
    21301 gaaatgggag agacagtgag caaaaaatca ctcattgtgc taacttgtgg ttgagtaaaa
    21361 gtcgttgtta tgaaagacca tgctgcgtta caggcgtagg agaagttagg gggcaaaagc
    21421 tagcatctaa gttaaggcat gtggatagat ataagggaac aggggaagcc atcaacatgg
    21481 agagtgcata ctcgatgaag tggtagtaag aaatatgtga atgacaaata acttacttta
    21541 gtgatggtga aagtagagtc acgactgatg aaggttctgt aatggaactg gaggaacagg
    21601 ctcgattcgt ctgtaaaagt catgaaaccc gttcaaccat gggattctcg tatgactaaa
    21661 aaggtcacga gccaattgga gcgtgataaa ccatgacaaa agaaacaagt ccaaaagata
    21721 ggaagttgcg gcatgcagaa tattatggca tgacggaaat cttcgataag atatactcca
    21781 aaagtttgaa gggagataat ttcaagaatc taatgcagat tatcatttct gataacaaca
    21841 ttgtcctagc atatcgtaat atcaagagaa atcacggtag tgtgacaaga ggagaagatg
    21901 gagtaacgat taaagatatt gaaaatctca accgtaatga attcgtagaa attgttagga
    21961 agcgattcgc acattataac cctcggaaag taaggcgtgt agaaattcct aaatataatg
    22021 gtaaattaag accgttgggt attccatcaa tatgggatag aatcgcgcag caatgcatct
    22081 tacaagtatt agaaccaata tgtgaagcaa aattcaataa tcacagctat ggtttccgcc
    22141 caaatagatc agcagaacac gcaatagcag attgtatggc ccgaatgaat caaagtcaca
    22201 tggaatatgt ggtagatgta gatatcaaag gtttcttcga cgaagtcaat catactaaac
    22261 tcatgagaca aatctggtct atgggtatac gagacaaaca gcttctctta atcattagga
    22321 aaatgttaag agcacccgta gtacttccaa atgggaagtt gatataccca acaaaaggaa
    22381 caccacaagg cggaatactt agcccattac ttgcgaacat caatctcaac gaatttgact
    22441 ggtggatagc aaatcagtgg gaggaaaaag atgccaaaga gctaaaacat cattttagga
    22501 aaaatggagc ttgggataaa ggtaatatct atcacaagct cagaaagagt acaactctaa
    22561 aagaaatata cctagtgaga tacgcagatg attttaaaat tttctgtaga aattataatg
    22621 aagcgaaaaa agttttctat gcaactaaaa tgtggttaca ggagcgtcta aagttaccaa
    22681 tatctgaaga aaagtcaaaa atcacaaatc tttcaaaaga agaaagtgaa tttttaggct
    22741 ttacgatgaa attagtgacc aaaggtaaca aaaaggttgt taatacgcat ataggtacaa
    22801 aagcacaaag catgattaaa gtgaaactaa aaaaacaaat caaacgaatt caaaaaagtc
    22861 ctaatagcaa agataccttt atctatattg gagtctataa cagtatggtt attgggattc
    22921 ataattactt caaaattgcg actcattgta gtgtagcaat gcgaaaaatt gcaagagaac
    22981 tcagaccaag ttttgaaata cgactacgag aacaaggaat ctcaaaagaa ggacaatacg
    23041 gaaagtacga aggatacaag ccttacctac ggtcaggaca aatacgatat ataagagggt
    23101 ttccaatatt accagtaggg tatattcaac acagacatcc gatgtgtaaa aaaagaaata
    23161 ttaataaata caccgaagaa ggtagggaag ccattcataa gaagcaacaa accgtagaag
    23221 aatggaagat atcgtggttg tgtagcaatc cagtgcttag taagagagca acgattgaat
    23281 ttgcggacaa tcgaatttca aaattcatcg ctcaaaaggg aagatgtgca gtaacaggtg
    23341 aggagcttat cttatctgaa atgcattgtc atcatattat cccttatcat gaaagcaaaa
    23401 gtgatagcta tgaaaaccta gtgattgtta ctgaggaggt tcatcgggta atacacgcta
    23461 cacaaagtga aacgattgag gaacttctaa aatatctaaa attaaatcca aaacaaaaag
    23521 aaaaactcaa tgagttgaga ttaaaagttg gtaacgaaga aatatcttaa taaatatttg
    23581 taggacggga aaacagaatg tcttttatag acgagggaac gccgtatgcg gtgaaagtcg
    23641 cacgtacggt gtggactggg ggaaaagacg gagataatat caaactctta cctatcagta
    23701 tcgattaatc cagattttat tgaaacggta aattatgtca caaactttca acgagaaatg
    23761 cgtaaagtag aagcaatgac tgttttagct acacaatccc cacaagaaat ggtgccagaa
    23821 aatatgtctt ctgataactt ctcgaaactg aagaaagtct ttgaattttc ttctacgaag
    23881 atctttatgc gtatggatga atctatttta cgtcatatag aaggattagt gggtaaatca
    23941 atgacgaact cagaattaga atccattcct caacaaaatc aaggagacgc tgtgatcaat
    24001 tttggctcaa aagaaaccat tcgtgttcat tttagtccga acaaacgtca attaaaattg
    24061 tttgatggag gaaaataatg gaaaaccaaa atgaaagttt aatcaagcag tatgttaagc
    24121 gtcgtgcaaa gcggcgctta tttttgtggt tgtttggtac ttctgcaggt ttgattacta
    24181 ttttgatcac tgtgttcgtg accctatttc taatacttgc ggcgggctcg attgataatt
    24241 cggattcaga ttcaagcagt ggtggcgaag cttttacagg agaatattcg gaaggcttac
    24301 cgatttataa agaaatcaaa ggaagaggcc ctttttctga tgagattgcg caatatgccg
    24361 taggtgcagc agtgaaatat aaattattac caagtgtgat cctttcgcaa tacggttacg
    24421 aatcagcgtt tggtacatcg gcgtccgcaa gaaatgatct caactatttt gggattactt
    24481 ggtttgatgg ttgtctgttt cctaaaggaa ctgcacgagg aattggtggc attgaaggtg
    24541 gctggtacat gaagtttccc aactcaaaag ctgcattttc ctattacggc tttatggtag
    24601 ccacacaatc caattttaat gcttgtgtag gaaataaaag ccctggcgca agcttattga
    24661 tcttaggacg aggcggttat gccgctgcag gaattacgga agatagtccg tattatacag
    24721 gttgtatgtc aattatcact tctaataaat taaccgaata tgatgaattc gccatcaaac
    24781 attggggtga aggtggcaat aacaacggca cgattactgg tgaatggacg aatccattcc
    24841 ctggcagcag tttagataaa agttccttca gcggtggtca attatttgga acgaatccag
    24901 gcggtgaatt ccgtccgaac ggcttccatg atggattaga ttttggttca gttgatcatc
    24961 caggaagtga gattcatgcg gttcatggcg gaaaagtcgt gtacgttggc aatcctggta
    25021 tttcaggttt gggtgcgtgt gtgatcgtga ttaattacga tggcttgaat atggtgtatc
    25081 aagaatttgc aaatagtaca ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg
    25141 gtcaagtaat tgggatacga gatacagcac acttgcattt aggatttact cgaatggatt
    25201 ggcgtcaagc acaaggacat gcattcattg atgatggtac atggatcgat ccgttaccat
    25261 ttttgaatag cagtaaaaaa tagatcagga gaaaaaatat ggaaaataaa acgttaaaaa
    25321 tcgtcttaac agctgcaatt agcgttagtt taatcggcaa tattgcgttg ttttggcagc
    25381 tgaaagagga acaagcagca aatacctata aaattgaaga agtggctaaa aacgcgtctg
    25441 agaagctcaa aaaaaattcc tctgaggcac aggcgaagat cgacaagtta aatcaaacca
    25501 tcaatgagaa aaaccaagaa atcgatcagc tgaagcaaca agatggcaca aaagcaaaag
    25561 aggaagtaac tgaagcacaa agaaagacgg cagaagagtt cggtcaattg gccattgata
    25621 aatcattaag cagaaatgaa ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga
    25681 tcgacaaatt aagtcctaca gacgatgatc accaacatga tgattacgga agttactcct
    25741 ttacattagg tgacgtgcaa acttacgctc aaacaagcag tgtaaaagag gaacaaaatt
    25801 ttgtcgtatt tgttgattat acaatcggca atccgcaatt caaagatgtc aaaccacaaa
    25861 aaataaaagg tggattaact gtcactgaaa aacaagtaga tggtcaatgg aaagtcatag
    25921 actttagtta tttcactcgt taggaggaag aaaatgagta agaaaaaagg agaaagacaa
    25981 cgtttcaaaa aacgaaaaag ttatcttaga tggctgggaa tctttatgat tctcggcttt
    26041 ttgattcttg ctagtccgat ttatacgaat cagaaggtag atataccaga tgaggtaagc
    26101 acgaacgcct ttttaaacac gaaagagctg acaatggttt caaaagaaaa aggagcgaac
    26161 cagcaattag tttttgaatt tgcgatcgat gatgaagatc aaagcttgtt aaaagaactg
    26221 gctaatctta attatcgcgt agaagtaaag acagctaaag gggattatca agcaatacga
    26281 gccaaagtga ttaaggtgtc tgatgattat tttgcagtga agctaacgca tgtaccagaa
    26341 gaatatatcg ccatgcgttt aaccattatt cctgaaaaaa ttgatccaaa agtggatatg
    26401 caagaaccac aagatctgat tttttatatt cacgaagata aggtcaaaga tcacgtgaaa
    26461 gacgaagatt atgaacaaca tgcgatcaat tacaaagtga aaggatacaa gaaagaacaa
    26521 aaagcagctc aaaaacaaat ggaatctttc caagcaacca ttgatttgaa tgaagaatta
    26581 gtgaagaagc taaaagatca attgccttat caagtcgcag atgatcagga aaatactgaa
    26641 aacaagatta atagctacca gcaagaaatt gaaacatcga aaacgcaact gaaagatcaa
    26701 gaagagatca ttcaaaaact tcaagaaaaa attgatcgta tgacaaaaga aaagatttag
    26761 aaggacaggt ggagtgaaaa aatgaagcga acaaacttgg ctgacaaaga tactttcgct
    26821 gagcagaaag tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcga
    26881 gaagtgaatt tatctaacac aacaatatct gagttgaaga atggtcgaaa gaatttagat
    26941 accttaaaat tcagatcatt tgaagctcta tatgtagctg ctaaaaaata ttttacagct
    27001 gataacaaat aaaaaacagg agtattttat gtgaaaaaag tatataaaaa atgcttatga
    27061 atcagctata tcaccttaac catttgttaa tgagatggga gaaaacatta accacgattc
    27121 taacgcaaac atcaagcaca taaaacattt aacactcctc ctttctggct gaagatttca
    27181 accaaacagg aagacagcgt atcaattata gcatacactt tttcttaaat tcatttttca
    27241 tatgacaaaa tatttccttc taatcgacaa agttagaagg attttttatt gtctaaatac
    27301 acaaataacc ttaattttta aagagcataa ggaaacgtga tatcactttc tcacgaaagg
    27361 tgttaggtag gctgtgccta ccacgtttct aagaaagata aatgatatca aaattttgga
    27421 tttgtgtgta gatttgtggg taggatgaaa gcgaatgtgg gcagtaaaat aagcgtttgt
    27481 gtgtagcaaa atcaaaaaat gtgtagggat atgtgtagct acacatatcc ctacacataa
    27541 taaatttgga ggaaaatatg gacttattaa ttagaaatat tgaagaaaag tatattaata
    27601 aaattgacaa acgttgtgaa gaacttacgg tcaagactgg caaaaaatgg agtcgaaatc
    27661 aatacctaaa agttttgatc gaaaatgatt ttgatcacgc tcttctgaat tataaaaaag
    27721 accaatttga tcggttgtta gaaaaatttg tggatattca aacttataat acgaaaactt
    27781 tagaagaata tattgcaaca aataatcaat taattggttt gttaattgaa taataaaaat
    27841 agtattgtga atttataaaa ggaggaaatc aattttggca aaagcaacaa gaaaacatgt
    27901 atatctttac gatatgcata tcgaagtact tgatgaaata atttctactc atgaagatat
    27961 atctaattat agtgaagcag tcagacattt gtgtatgatg tatgatccaa aaatcgaaga
    28021 aaaaataaat ccaattcaaa ttaaattgaa tgcaatggga aaagaaattt ctattttaac
    28081 cgaaatggtt tcagagtttg cggatcttca tttgaaagat acaggaatac taattggtac
    28141 aaattcaact gtttatcaag aagcagaaga acgtgtggaa ggaaaaatta aatccaatca
    28201 aaccaaaaaa tattcttcta agttaaagaa accagtgaat gaatctgttc ataccagagt
    28261 agatccattt gatccaacga agattctaaa aagataaaag tagaaggaga acgtgatgaa
    28321 taaatcgcct gcagttattc ttacttgtca atttaccttg cccaataaaa aatctttttc
    28381 aacatacatt gattatatga caagagaaaa agcactggaa gaaatggaac gacgtacgcc
    28441 cgaagaagaa caggaactgc aacgaatcaa gaatgcttta gaggattttt atattcctga
    28501 aggagaaact ttttcggaaa gtaagacgga taaagaattc agtgaaacgt cgaatgaagc
    28561 tcaatcgttg atggaacatg gaatcaattt tgacaagtta gaggaacaag attttaccaa
    28621 atatctttct tatatgaccc gtcactatgc gttagaacaa aagaaagaac tgacgcaaag
    28681 ggaacaaaaa gaataccaac ggttgaacca agcaatgaaa aaatatcaaa cagagccaca
    28741 taaaaaagct tcaggaaatc ttcctggagt tttttctatg gcttcagacg aagtgaaagg
    28801 aagtgatctg aaggagatca aacaaatctt ccaaagagga caacaaaaag gctcaatcat
    28861 ttatcaagat gttatctctc acgacaattc gtatcttgaa aaaatgggct tgtacgatcc
    28921 caaaacagat accttagatg aagaaggttt gaaagcagct ggaagaaaaa tgatggagac
    28981 attattcgag aaagaacaat tgaatgaaac aggttactgg atggcaacga ttcaccgtaa
    29041 cactaagcat atccatattc attttgcagt tgttgaaaaa gaaaatacgc gtaaaccgta
    29101 tactgagatt gaaaatggtg tgagttatat cgtaccgaag gggaagagaa gacaagcaac
    29161 attagacgca atgaaaacca attatgtaca tgagttagaa cgtttctctt atgaaaagga
    29221 tcgaatgatc ggtatggaga aacgaaattt attgacacga aaaagtgatt tgcgtaacac
    29281 cttaacaaaa gtagtaaaag aaccagcacg ttacgatcga cacgccatga acttattaaa
    29341 tgaagtatat aaaaccctac ctgaaaaacg ttcggaatgg aattatggag atgaaaatcg
    29401 aaccaagctt tcaccattta caaggggaaa acttgatgag ttaaccaaat atgttttaac
    29461 aaaagaacct ggatatcaag aatatataca gatttcaaaa gcattgaaag aagaagccaa
    29521 aagcctttat ggggaaagca aaagagaaac aaagaatgca gaaaaaaacg ccctctttga
    29581 tttaaaaaag cgcacaggaa atgcgatttt aacagaactg aagagacagg atagtcaaat
    29641 aaaaccatta atagaaagtg tcaatcagat gcaatctatt tacaatgaac gatcctttcg
    29701 aacactcaaa cagttgccaa caaatgaata caagtatagt tatcaagaat tcgtaaagcg
    29761 taggaaacaa ttccaacaaa gagttgtcac gcagcgaagc ttgcgtcaat taaatcgaac
    29821 aattaatgca catgaagaaa aataccacgc aagtaaagca tatgaagagc aacagcgaag
    29881 aatccaaatg gaacaagaaa gacaatagga ggaagagaaa tgaaacaata tttagatcaa
    29941 tggaaagtca tagaaggatc actaaaagaa gaacgaatcg aacaacttcc tgattgttta
    30001 gaaaaagagc atttgtttca gataagcgaa atgttgagaa acgaacagtt tgatccaaat
    30061 caatttttag ttgttgagta cccagctact ggtgtgtatt gttgtaatca tgtgaagggt
    30121 gaaaaatatt ttattattca agaatacgaa ggtaagttag cgccttatta tactacttgg
    30181 gaaataaatg aagagggaat caataatttc ccatgtgaaa gcattgaaga gtctatttct
    30241 ctcacggaat gttaaataaa tagagacgta aaagagtcct gaacgtgatt gtccaggact
    30301 ctttttcaat gaattgtttt ccgccaattc actgagtggt gcgattcaat tttattatat
    30361 caaaagaaag tgagattcca aagtgaaggg aggaagaaga aatgactcag acaacgattg
    30421 gtgttgtggt tgaaaaaagc agggataatc tcgtttttgt gacagaaatt caaacaggta
    30481 gagcttttgt agtcacagac aaagctgcaa aagcgtatca aaatggcaat attttaacgt
    30541 taaatctgac tacgaagatg ttcgttgatg cagtagaaaa ttatccattt gtttagacaa
    30601 acgatatttt acaaggagga aacggacatg gcagaagaaa aagaaccact agataatagt
    30661 cggttgggaa agtccaaaag aaaattagta cggttgcaaa acgaattaaa taatcaattt
    30721 caaaagctat ttgatcacca aaaattgaca aatgggcaac caatgaatga caaacgaaat
    30781 ggtcgttcat ggtttaacca gcaagaaaaa atagaaaata aaatacaaag tttgagagaa
    30841 gagatcaagc aacaagaaaa acaggtagaa aaacttgaaa gacaagaaga gctaaaggaa
    30901 atgggctata acaagtacgg cggactggat atgacgatcg ataatattcc aagaatcaaa
    30961 gaagaaatcg aacgctttga aaaaggtgaa tcgacttttt cagccgccac gatccgaaaa
    31021 tatcaacgga agttggaaac attagaacag ctgaaagaaa aatcagaaaa aggaaaagaa
    31081 aatatcctcc ctgaagtaca agcgatcatt gacagcggac gagtgacgca atggaaaaaa
    31141 aatccaacca tttatttttt gaaaggctat cgaaaagttg ctttagaact gaatgaaaac
    31201 gggggatttg aggaatcaaa aaaataccaa gccacgtcgg aagaggaaaa agcagctgtt
    31261 cgtgaattac ttgaagaggc tgcaccaact gattctctga aggagaaaga aaaagatcat
    31321 ttctatcgag tagaattcaa cgaaaatgat ccagaaaaag gattgaaaag ttatggtggg
    31381 gagatcgtaa cattagaatt gatcgatcaa cttaaaacgt tggataacca atacgctgca
    31441 gaagcaggtt attataaatt ctattttgaa gaagtgaaga acggagaagt agtagatcag
    31501 atccgaatgg atctagggga tggcgtcaac agtaatcacc ccatctattt agagttagca
    31561 aaattgtgcg aacaagtacc tgagaaggaa caaaaacaat cggtcaaaaa tgaacctgtt
    31621 tcgaaagaac aaatgcagtc gaaagaaaca aaaacaaaac aaaagggcta tcaaaaacga
    31681 acggcagaag agatcaaaca agaagttaaa tccctttcgc aacaggcact ggatgcggtg
    31741 aaaaagcata cccattcttc tgaagacgtc aaagaacttt tgaactttat gtctcgtttt
    31801 ccagaacgat cctttagaaa tcaattattg attgaggaac aatttccagg tgcaacggct
    31861 tgtttaggtc gtgccgcgtt gaaaaaagaa ggaatctata tcaaaaaagg ggaacaaggg
    31921 aacaagatct ttgtcagaaa aacggtcaaa ggattttatg agaaaggacg tggctttgtt
    31981 cgagaaacag aagctacacc agaagataaa aaacgaatcg aaagtggtca aatcgaagtg
    32041 atcaaaaaac cttactacac gattgaaaaa gtgtttgata tcacacaaac acagttaaaa
    32101 cctgaagact atccgaaaat ctttccgaac cgtgtctttg attttcaatt ggataaaaaa
    32161 ggacagtcag aacttcaagc tgggattcaa gcggtagcca acaatattgg aatacaaatt
    32221 cgagatatga cagaaagtga agtctatcaa agagaattag gacaagcacg tggggcgtat
    32281 gttcgtaacg aatttactgg cgaagaagaa atcgtcatga atactcgaaa tactcctacg
    32341 cagcatttag ctacaagtat tcatgaatta gcccatgcaa gaatgcacaa attttcagaa
    32401 ttagataccg caactaaaga attgcaggca gaaatgactt cttatattgt gtgcaaacat
    32461 tttggaatgg acacttcgga gaaagctatt ccttatattg cagcatggac gaagaatggt
    32521 cagacgttgg atgataagga agcggcggaa cgtgggaaaa tcatgaatga tgtcagccgt
    32581 gtcgcaaatg aattcattca aaccatttct aatgaaatca atcaatacag agaaagagaa
    32641 ccagagatcc aaccagcaga agtgaagcag gagcataaaa atactgtaga aacggcagag
    32701 aataagaata ttgtgcccgt tggttcactt tggatcgaca agaaagacgg gaaagtaatg
    32761 gaagaagata cgggtcgaac gttacatttg aaggatgcgg cgtttattga agatccgcag
    32821 cataatcaag tgattttaaa aggaaaaagt tatggaaaag agattcaaac ggcgttacct
    32881 tcagaagaat ttgcgctagg aaatccattc cgattttcac ttgaaacagt gtcagaagtg
    32941 gccaaacgaa aaaatgaaat ctattatgaa caacaattgt cacaaaaaga acgcgtataa
    33001 aacaagtaaa aaagactatc tgaaaaacgg atagtctttt ttcttttggg aggtttgaaa
    33061 atgaccgaaa aaaaaatgag tcttattgat cgatgtaaac agatcgatat agtggatttt
    33121 gctcgaaaca atggaatggc tgtagtcaat aaaggacgag actatcgctt agaagaccat
    33181 gattcatttg tttttgatcg aagaaaacaa cgtttttatt ggaacagtca aaatattagt
    33241 ggcgacatta ttgaattagc taaacttttt ttcatagaca aagagatcca agactctaag
    33301 caacaattta aagcagccac tgattttatt ctaaagaatg aagacaaaac agaacgagtg
    33361 gaaaatcttc attttgaaac agaaaagtac aaagatcacc ccgttgatta tcaaccactc
    33421 acagaaaaag gaaggaatta tctgaaggaa gaacgaaaac taccagagtg gctgattgat
    33481 tatgcagaaa aagaagggtt gattgccgaa ctaaagccaa aatatgaacg gcaaaatttc
    33541 ttagttcgag acgatcgttt agatcatgcg gtcgcctttt tatggaaaga tccacagaca
    33601 aaagagacag taggggcgag ttatcaagga acgttcattg attatgaacg ttttggtgag
    33661 agaggcacat ataagcatat agataaaaat tcgacagcga atcatggctt taacctaaag
    33721 atcggtgacc ctaagcagtt aaaatttttt gagagtagta ttgatttact tagctatgct
    33781 gcactgaatc gtgatcagtt aaacgatacg tggttagtct ctatggaagg gttaaaacac
    33841 catgtgatca gtcattattt tggtgaggca gtatctgaat tgagaaagaa acaagccttt
    33901 cctcaatcca tcgagatttg tgtggataat gaccgtgcag gacatatttt ctacgaaaaa
    33961 gaacaattaa tgggcgcagt cgatccattt acgaatcaga aagtacgttg tgaacgagga
    34021 atcgctaatg attggcaagt gccaaaagaa tacaaaatca tttatgaaga agtcgcaaaa
    34081 gaagaaaagg tcacaccaga agctattatg gccatacaca agacagaaaa caatctgcaa
    34141 ttaacgaacc aactggtttc cgctcacaaa gtaaatgcgt tttttggcca gcagctttct
    34201 gttaacgatt caatagaagc cattaatttg aaagatattt gtagagaagt cgcaaaagaa
    34261 ttaaaagttt gtgaacgcgt ggatggtacg tacgattttg atcgttttta tcaagaaaaa
    34321 ggggatatta atgcgcagat tcttttctct tataaagcag aacaatacta caaaggctat
    34381 aaaaatcatg aacatgaatt tatacctgaa gtaaaaaagg actggaacga tcaattgaag
    34441 catgagatcc agcaacaaga aatcagaaag caaaaacgtg ccatgttgtt tcaacaaggc
    34501 agacaacaag aaagggagtg aaatcgtcat gatgaaaacc gttattttag cagaaaagcc
    34561 gtcacaagcg aaagcttatg ccgatagctt ttctaaagct acccgaaaag atgggtattt
    34621 tgagatccaa gatcgattgt ttacaggaga aactgtgatc acgtatggtt ttggtcattt
    34681 agtcgagtta gattcgcccg atatgtacga tgaaaaatgg aaacaatggg cattagaaca
    34741 tttgcctatt ttccccaatc aataccacta tcatgttcct aaagataaga aaaagcagtt
    34801 tagtgtcgtc aaacgacagc tgcagtcagc tgatacgatt gtgatcgcga ctgatagcga
    34861 tcgtgaaggg gagctgattg cctggtcaat cattcaacaa gcaggcgcca atcaaggaaa
    34921 aacctttaaa agactttgga tcaactcctt agaaaaagaa gccatttatc agggattcca
    34981 acagttacga gacgcagaag agacctatcc aaaatttgaa gaagtacaag cacgtcagat
    35041 cgccgattgg ttaattggga tgaacggtag cccactgtat agtttactct tgcagcaaaa
    35101 aggcatacca ggaagttttt ctttaggtag agtccaaacc ccaacactct atatgattta
    35161 tcaattgcag gaaaagatta gaaacttcca aaaagaacca tattttgaag gaaaagccca
    35221 agtaatcgct caaaatggcg catttgatgc gaagcttgat ccaaatgaaa cccaagcgac
    35281 acaagaagca tttgaagcct atttaaaaga aaaaggtgtt caattaggaa aacagccagg
    35341 tacgatcctt caagtcgaaa cagagaaaaa aagtgcggct agcccacgtc ttttttcttt
    35401 atcgagcctt cagtctaaaa tgaatcaact catgaaagct agtgccaaag atactttgga
    35461 agccatgcaa gggttgtatg aaggaaaata tctaagctat ccgcgaacgg atacccctta
    35521 tattacggaa ggggaatatg cgtatctgtt ggatcactta gacgaataca agcatttttt
    35581 aaaagccgaa gcgattccga ctcctattca tacaccgaat agtcggtatg tgaataataa
    35641 gaaagtccag gaacattacg cgatcattcc tacaaaaacg gttatgacag cagcggcttt
    35701 tgaacaacta agtccactgc agcaggctat ttatgaacaa gtgttaaaaa caacggtggc
    35761 catgtttgcg gaaaagtata cctatgaaga aacaacgatc ttgacgcaag ttcagcagct
    35821 tcagttaaag gcaatcgaaa aggtgccgct agatctaggt tggaagaagt tatttggaaa
    35881 agagagcgac ggaaaagaga aggaagaaga gccgctactt cctaaagtga caaaaggaga
    35941 aatggtgacg gtggatctac aagttcttga aaaagaaacc aagccaccac agccttatac
    36001 cgaaggtacg ttgatcacgg cgatgaaaac agctgggaaa accgttgata gtgaagaagc
    36061 gcaatccata ttgaaagaag tggaaggaat cggtacagaa gcgacacggg cgaatattat
    36121 tgaaaccttg aaacaaaagg aatatatcaa ggtagagaaa aataagctcg tggtgacgaa
    36181 taaagggatt ttgctttgcc aagccgtgga aaaagagccg ctgcttacga gtgccgaaat
    36241 gaccgccaaa tgggaaagct acttgttaaa gatcggtgaa cgaaaaggaa cacaaaccac
    36301 ttttctagcc aatatccaaa aatttgtctc tcacttattg gaagtcgtac cagggcaaat
    36361 ccaatccact gattttggat cgacgttaca agaagtgaaa gcagccagtg aaaagcaaga
    36421 agccgcgcgt catttaggaa tttgtccaaa atgccaggaa caagaagtct tactctatca
    36481 aaaggtggca gcatgtacgt ctgaagcctg tgatttcaaa ctatggacaa cgattgccaa
    36541 aaagaaactt acagcgacac agctgaaaga gatcattcaa aatagccgaa caagccaacc
    36601 agtgaaaggg ttaaaaggtc agaagggttc gtttgaagca acgattgttt taaaagaaga
    36661 cttcactaca ggctttgaat tttctgaaaa gaaaaaaacg aacttcaaga aaagaacacg
    36721 acgcactaca aaataatgga gggttaatct tatgcgtaat acaacacaca aacaacaaga
    36781 aatcgaaaat caaaaagcca aaattcgttc agaacaggaa aaaatgcgtg ccagcaaacg
    36841 aagactgcag caattgaaga accagcagcg gattcaaaaa gaaatcgaat tagatcgata
    36901 aattcctttc ttgttaatat aaaactaaca tgttatagtt gtgttagttt tatattaact
    36961 ttaggggaag agtaatgaaa gacaaaatcc aatgtatttt tgataaacat aatggcaacc
    37021 tatccttgaa ggatgctaga agtgaaggat tatcgcctat gacgttagat cgattagccg
    37081 aaaaggataa aatagaaaag gtagcaccag gtttctatac cttaaaggat gcgttcgtgg
    37141 atgaattgta tctcgctcag agtatctatt ccagagggat tttttctcat gagactgcgc
    37201 tcgatctttt tcaagtagga acatatatcc ctaaaaagat caatatgatg tttcctaaag
    37261 gatacaatgt atcaaaggag aaattagatc agtatgccat tcaaccgcat tacactaata
    37321 aagaaaattt tgacctggga gtcacagaaa cagaaagctt ttacggcaat aaaattttag
    37381 tctatgattt agagcgaacg ttatgtgaca tgtggaatcc aagatacaaa gcaagcgtag
    37441 aggtaaaaca agaagcatta aaagaatata tgacaagtac cagtagaaat acaaataagc
    37501 taagaagata catggatatt ttaaaatcac caaaagaaat gactttgtat atgttgccac
    37561 tttattgaga ggagtaaaga tgaatgacac cgacacaatt taaagagaaa acaagaaatt
    37621 tcgcagcaga gaaacattta aacccacaaa ttgtccaaag atattatatg atggaaaaat
    37681 tattagaatg tattgttgaa tcgccttatc atgatgattt tgtattaaaa gggggatttc
    37741 tgattggatc aaaatacggg ttagaaaacc gttcaaccgt tgatattgat acgacttatc
    37801 gaaattcgaa actaacaaaa gaaaagttag aaactgtttt ttctgaatta acagctaatc
    37861 ctacaaaaga tggaattcgt tttgaattaa agggattaac agagacgagg gaagccgatt
    37921 attatccagg ttttcaagca aagtttattg cttatcttga aaatgcacgt gtacctttta
    37981 aattagatat tacttcaggt gattctatta ttccagcagc attgatgtat gaacacaaat
    38041 taatgtttga agataaaacg attcatattc cagcttatcc tacggaacaa attctagctg
    38101 aaaaattaca tgccacgttt agttttggaa aagataattc gcgcatgaaa gatttttatg
    38161 acctgtatat gattccgaaa ttagaacgta tcgatgagaa aaatttgtat aagagtgttc
    38221 aaggaacatt caaggaaaga aataatcaat cggtgtttaa gcatttgtat gatcaggaaa
    38281 tgcctcaatt ggtgaaagat gagggcttga gagaaaagtg gaaaagatac caacaagaaa
    38341 actattttgc caaagacatt tctttcgaat ctacactaac ttccgttgat tccctgatgc
    38401 aatcaattgt agagcaagag aaggcagaac gaaaacaaaa ttttaagaga tcacaacaaa
    38461 tgaatcaaga aatggagaga taaagatgat caataatgta actttagtag ggcgattaac
    38521 taaagatcca gatttacgtt ataccgcaag tggcacagca gtggcgactt tttccctagc
    38581 cgttaatcgt aatttcacga atcaaaacgg caatcgagaa gctgatttta tcaattgcgt
    38641 gatttggcgc aaaccagcag aaacgatggc gacattagct aaaaaaggca gcttgatcgg
    38701 cgttgtcgga cgaattcaaa cacggactta tgacaaccag caaggacaac gtgtgtatgt
    38761 gacagaagtt gtggccgata acttccaact attagaaagt aaagcagcga cagaaagccg
    38821 tatgcacgct gatcaatcgt ccagcagtcc aagccctacc acctttgaac aacgggatac
    38881 agcgacgcct aacaacaatg gattgaacgc ttcacagaat ccatttggtg gacaatcaat
    38941 cgatattagc gatgatgatc taccgttcta agtagcattt tctattggtt gcatttgcta
    39001 cggtttatgt tataatttag acaacaaaaa gaattttcaa tctataacca aaaaccacta
    39061 gctggcgggc tagtggcttt cttttacgtt tctattataa gaattaacgg gtaggcttac
    39121 tgtctttctt ggcaaattta caatcaagcc atttgcagat atagtgcgcg gctacttctg
    39181 ccatgattgc aaaaacaaaa tcaaaaagaa ttttcaaacc tataacctcc tccccttgcc
    39241 agattaggcc ggggtagcag acagcaagac tattatagca taattgaaat atccaaatga
    39301 ttggattttt gctataattt tagcgaggtg attgtgatga acaaatatgt tggtttgctt
    39361 gataaaatcc gagtaataaa aacgcaaccg ttattggttc gctttacttt acaaacgatt
    39421 catgaatcga ttaattgtgt agttgcagat attgaaatta tcgataaatt gctaattatg
    39481 gatgatggaa aatacaatat tgccgtcaca ggtcacttca ataaacgaaa tcagctagtt
    39541 attgctagca tgtatgttag aaatcccgat cattttacgc gatcgatggg catataaata
    39601 aaagaagagt tgtagtccta aatgaggatt gcagctcttt ttgttttaca tagaaacagg
    39661 aaaagacctt tttaggaaga cacaagtgcc ttctcaattg aaaaaccttt ctctcactcc
    39721 aaccgccacc gccgttcgag aagaaaggaa gtgaacgctg cacgttcact aagatatggt
    39781 gtcttgcaaa gcaagacaaa atagacaaaa cttcctctca ccaaaaggtt cgttcctgtt
    39841 cgttatctct ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg
    39901 acaaggacaa gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattt
    39961 attctggcca acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag
    40021 aggaggcaga atcaaacatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat
    40081 tattcccaaa ctatcatgca ccacatactg ttgaatgcga ttgtggagaa agagcgaaat
    40141 gtatcaaaag ctatcgtttg tatcaatgtc caacatgcgg gaaaaaatat accttgtctt
    40201 atggcgatta cattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cacaacacaa
    40261 attattgact gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa
    40321 agtagcagaa gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga
    40381 tgcaccgact atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca
    40441 agaaaacatg gaaacttatg aaatcgaata ttggttaacc aaattagaga actaaaaagg
    40501 agaaaaaata tggctcaaaa ttactatgac gaatttgtca aattaccttt ggataaaatg
    40561 gcacaaaaaa tggaggatat gacgttccta taccatgaaa cacgtgtacc caaaaaacat
    40621 tacaaggaaa aattgtcagt cgcagtggaa gaaatgattg aatcaggtgt cgaaatgaat
    40681 ctcattgcga cgtactatcg aacactggaa gaactgaaaa aacaaaatgg aaaatggttt
    40741 ttccaagcct tactatgctt ggaagtaggt gtcaaaccaa gcactatcaa accttccgaa
    40801 tatcaagcct tagaactgac ttatgcaaaa ttcgcacaga ttaagaaagc taaagcaatg
    40861 gctagcgact ggctagatac ttttgaaagt atcgaaaaaa atggtgcatt ttatacgatt
    40921 gaatcggagg agaaagataa tgaataaggt ggtttatcag ctaacaacag aagatgtttc
    40981 gcaattttta acaagcaaag aacacttgaa tgaagtaatc agacggattc aaaaagactt
    41041 tagttgtatg gaagaaatga agtactttat tcaaagagtg gaagaacagt tgtatatgga
    41101 aagcgaaaca agtcaaacga aggtcacagt cagccataat catttagacg tgtataaacg
    41161 tcgtcctgaa acggtaaaaa aaggaacaga gcttctacga ctagaaacag aaaaatacat
    41221 catgaatgtg tacctagaaa ggagtgagct ggtcgaaaac tttgtcagct acacgatttg
    41281 taggaaacac tattactcag aatcagaata ttattcaatt ttcgagagca tggaattaat
    41341 ggaagaagaa atggaagagc taaacagcaa aaatgcgacg gtagacgatt attaccttgt
    41401 atttactagg tttttgacgt gtgggacgga atctatcgtg ttaatgggaa tggattatga
    41461 actcgctgta cccgaagcat ttttgaggga atatgtagaa gaagtagata acccaggcga
    41521 cgactggtac acgactttaa ctgatttttt agacacctac acttacgacg atacagaaga
    41581 aatccaaaat gaacttgacc gtaaacgaat gccatatcaa cgatttgaaa ggaaaaaagg
    41641 taaataacca tttataccga aaagtgagga tgtgtgaaat aagaacaaaa aaccttgaaa
    41701 ttaagggaat ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt
    41761 gcgaaataag cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa
    41821 atcattcctc tattgttcgt actgtttgtt ggcgagatac ttatgcgtca tgctgcagct
    41881 gtacatttaa acttcgtagt attcttagga atactggtat tttgtttgat cttttggcga
    41941 tttgtattag cccttgtgag tctactattc aagatgattg cagccatatt tttcataggg
    42001 ttatttcgat gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc
    42061 gtgacattag atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa
    42121 agtgaatcta agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga
    42181 tttgatatac cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata
    42241 gcacttaatt gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat
    42301 caataactat gaaatcttta aagtatccag aagagattat ttttagatca atttttttct
    42361 tacctgattc taaagatttt gttttcttaa acatattatg atttcctttc gactattttc
    42421 atataaatgt taacatagtt tatggattca tggaatgtta atctaattta atagaaaagg
    42481 ataattaagt atatacgttt tttcttatgt atgttatact atgtatatac agaaagaggt
    42541 gtctactatg gaaatgttac ctataaaaaa atggggaaat agtaatggcc ttagattgcc
    42601 aaagtatatt atggaatatc ttggaattca tacagaagac aaagtgaaga taatacaaga
    42661 agaagtaaac ggtcagaagc ggctaattat cgaagcggct aatatagaaa atgaattaac
    42721 aattgaacag ttatttgaaa attacactga tgaaagaaat catgtgacga ttcaaaattt
    42781 aggtgaagca gtaggtaatg aaaaatggtg agaaaaccta gacaaggcga tattctatta
    42841 ttgaatacag cacctcgatc tggacacgaa cagacaggta aaagacctta tattgtttta
    42901 agtcatgata ttattgcgga ttatagtaac gttgttacag ttgctccaat ttctagtaca
    42961 actagaaact atccgctata tgtaaatata aactcagaat atgaaatgaa gacaacagga
    43021 aaagtattat tagatcaact aacgacaatt gattatgaag ccagagagtg tatttttttg
    43081 gaaaaagcgc atgatagttt agttgaggag ttattgatga aagttagaac agtttttcaa
    43141 aaagtaagca aataaaatag aaacagcagc atgatcaact attcgtgatt gaccatgctg
    43201 tcttttttta ttctatatac ggtttgtctc gtaacattgt agtcttttgc aatcttagcg
    43261 atcgcaacac ctttttttag atcctcaacg atgtttttat aaacaagacg acgttgggga
    43321 tcttttgcat tggcactgta tagcttagga cgtcctttat aaacaccgtt cgcttttgca
    43381 atcttaatgc cttgtgcctg acgtcgttta gattcggtac gttcttgttc agcgatcatg
    43441 gccaaaattt gaatgatcaa atctttaatg aatttatcca ggagaggatt gccaattgct
    43501 tcggccatga tcggtagact tgtgatgatc agctgcacat ttttcttctt caagtaattg
    43561 actgaagcga tgatctcgtc gtaatttctt ccaagtcgat caatagcttc cacaataaaa
    43621 atatcttgat ctcggacgaa atccagtgct tcttgaaaaa caagtcgatg agtaatcgtt
    43681 gcgccagatt gtttctcaat gaaaattctc tctgcaccga attttttcat ttcttcaatc
    43741 tgccgttgtt cattttgatc gatggatgag acacgtatgt atgcaactct caaaattttt
    43801 acctactttc tgcaactatt taaaaaacac cctaatcata cacgagaaaa ccagattttt
    43861 caatgtcgcg gtcgtgtcta gttagggtat accttatata gacaaaaact aatgatggag
    43921 gaaatagtat gtgtcacttt tctttttaaa tggcaatatt ttttgtaaat aaatgtcaat
    43981 caagaaaatt taaacgatta tgacattaaa aatatatttt atgaattttc tagtatataa
    44041 cttgatttat ctgttaagat ttattaagaa aacgttttct tatttttgat ttcgaggtga
    44101 tagacaatga aaaataagtt tattttatct ttaggtattt taactattgg aattagtgta
    44161 actggaacta caattcctac atttgctgat agtacaaatg aaccaactac tgtagtagat
    44221 aataaccaag aattaactcc tttagatagg aacggaatag ccattggtaa cccaactaac
    44281 gacttaactg ttactgaaaa ttatttaata gtttctccat tacttgctcg agcagcttct
    44341 aaaacgatac gagtgacagg aacgatgatt tttgataaag ttggttggcc ttctagacct
    44401 cctcaatcac ggaattggaa agaaaaaaga aatggttatt ggtactatgg aaaattgtat
    44461 ttacaaagtt ataaatcagc tggaaacaat tggattgcga cttattcagg aaccttaact
    44521 agaaagtagg gattttttat gccagaattt gtgaacacac caaattttta tcaagatagc
    44581 gatattctaa gtaaaattaa tttaccgaaa gaagaatcag ctttacaagt tcagcgaact
    44641 ttacaggaga cacaaaaaat tcaaaaatca atttctaatc tagaagattc tgaagaaaaa
    44701 agaaaaaaaa gagaccaaaa aacatatcaa aaagaaaaat aatgatttta aaaaaagagt
    44761 gtctagtcga cattcttttt ttctaattga aagtgttcat aaaatcaccc atttctggac
    44821 actttctttt tttgctttat ttttcaaaaa aacaattcat ttcatgttca taaacccata
    44881 acaatatcta tgttcagtta aactatatgg ataaagttat gttacaataa aattaatgaa
    44941 ggagtgattt ttttgaaaat aggatacgcc cgggtatcta ctggtttaca aaatttggat
    45001 ttacaagaag acagcctaaa taaatttggt tgtgaaaaaa tttttactga tcatatgagt
    45061 ggggcaaaaa gtaatcgacc aggattagaa atggccattg attttgttcg ctctggcgat
    45121 acacttgtcg tttggcgctt agatcgttta ggtagaaata tggaagattt gatttcaatc
    45181 gtcaatcgac tgaatgagcg tggtgtgagt tttcatagta ttcaagaaaa tattacgatg
    45241 gacaaatcaa gttctacagg acagctcatg tttcatttgt ttgcggcatt tgcagaattt
    45301 gagcgtaatt tgatcctgga acgatccgca gcaggacgag cagctgctcg tgcaagagga
    45361 cggtttggtg gtcgtcctga aaaattgtct gaacaagatt tggaattact gaagacttta
    45421 gtggatagcg gtacacctat taagactatt gcggaacggt ggaacgtctc aagaaccacc
    45481 atttatcgtt atttggcaaa aatggaggag aaaaatggaa agacaaattc tcattagttt
    45541 ttgtcattta atgctgaaaa tttttgccat ttatctagaa aagtgacaat agtatgagaa
    45601 ttttttgtaa gtgtctactg ataacttatt ctcaaatgtt gtaaggattt tattcatttg
    45661 ttaatattaa
//
LOCUS       DAZKLA010000007        44836 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000007 DAZKLA010000000
VERSION     DAZKLA010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.007"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACG85N_000305"
     CDS             212..604
                     /locus_tag="ACG85N_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0223106.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACG85N_000306"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACG85N_000306"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFQ0223107.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACG85N_000307"
     CDS             2698..2943
                     /locus_tag="ACG85N_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223108.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACG85N_000308"
     CDS             3165..4775
                     /locus_tag="ACG85N_000308"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFQ0223109.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACG85N_000309"
     CDS             complement(4752..4964)
                     /locus_tag="ACG85N_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223110.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACG85N_000310"
     CDS             5067..5936
                     /locus_tag="ACG85N_000310"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFQ0223111.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACG85N_000311"
     CDS             6101..7372
                     /locus_tag="ACG85N_000311"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0223112.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACG85N_000312"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACG85N_000312"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFQ0223113.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACG85N_000313"
     CDS             8763..9026
                     /locus_tag="ACG85N_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFQ0223114.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACG85N_000314"
     CDS             9471..9920
                     /locus_tag="ACG85N_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0223115.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACG85N_000315"
     CDS             9977..11395
                     /locus_tag="ACG85N_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0223116.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACG85N_000316"
     CDS             11395..12255
                     /locus_tag="ACG85N_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0223117.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACG85N_000317"
     CDS             12429..13202
                     /locus_tag="ACG85N_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0223118.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACG85N_000318"
     CDS             13349..13714
                     /locus_tag="ACG85N_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFQ0223119.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACG85N_000319"
     CDS             complement(13862..15070)
                     /locus_tag="ACG85N_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0223120.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACG85N_000320"
     CDS             complement(15089..15367)
                     /locus_tag="ACG85N_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223121.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACG85N_000321"
     CDS             15560..16216
                     /locus_tag="ACG85N_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0223122.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACG85N_000322"
     CDS             complement(16658..17455)
                     /locus_tag="ACG85N_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFQ0223123.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACG85N_000323"
     CDS             complement(17452..18636)
                     /locus_tag="ACG85N_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFQ0223124.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACG85N_000324"
     CDS             complement(18661..19908)
                     /locus_tag="ACG85N_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0223125.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG85N_000325"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG85N_000325"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFQ0223126.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACG85N_000326"
     CDS             21888..22202
                     /locus_tag="ACG85N_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFQ0223127.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACG85N_000327"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACG85N_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFQ0223128.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACG85N_000328"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACG85N_000328"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFQ0223129.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACG85N_000329"
     CDS             23546..23875
                     /locus_tag="ACG85N_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFQ0223130.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACG85N_000330"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACG85N_000330"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFQ0223131.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACG85N_000331"
     CDS             24863..25687
                     /locus_tag="ACG85N_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFQ0223132.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACG85N_000332"
     CDS             25680..26027
                     /locus_tag="ACG85N_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFQ0223133.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG85N_000333"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG85N_000333"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFQ0223134.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACG85N_000334"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACG85N_000334"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFQ0223135.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG85N_000335"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG85N_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFQ0223136.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG85N_000336"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG85N_000336"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFQ0223137.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACG85N_000337"
     CDS             31684..32451
                     /locus_tag="ACG85N_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0223138.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACG85N_000338"
     CDS             32441..34483
                     /locus_tag="ACG85N_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0223139.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="ACG85N_000339"
     CDS             34764..34913
                     /locus_tag="ACG85N_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFQ0223140.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="ACG85N_000340"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="ACG85N_000340"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFQ0223141.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="ACG85N_000341"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="ACG85N_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFQ0223142.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="ACG85N_000342"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="ACG85N_000342"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFQ0223143.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="ACG85N_000343"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="ACG85N_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFQ0223144.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="ACG85N_000344"
     CDS             39330..40412
                     /locus_tag="ACG85N_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFQ0223145.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="ACG85N_000345"
     CDS             40412..41215
                     /locus_tag="ACG85N_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0223146.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="ACG85N_000346"
     CDS             41285..42025
                     /locus_tag="ACG85N_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0223147.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="ACG85N_000347"
     CDS             42036..43040
                     /locus_tag="ACG85N_000347"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFQ0223148.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..>44836)
                     /locus_tag="ACG85N_000348"
     CDS             complement(43085..>44836)
                     /locus_tag="ACG85N_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223149.1"
                     /translation="TGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDSKYTR
                     TIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGL
                     SLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMI
                     IAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHS
                     FLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELM
                     PIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTT
                     INWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEV
                     NTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARY
                     QKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSI
                     HEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDT
                     FIDRITVQLSRNYPNPV"
BASE COUNT        14406 a         7826 c         9340 g        13264 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgt
//
LOCUS       DAZKLA010000008        44468 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000008 DAZKLA010000000
VERSION     DAZKLA010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.008"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1879)
                     /locus_tag="ACG85N_000349"
     CDS             complement(<1..1879)
                     /locus_tag="ACG85N_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0223150.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VE"
     gene            complement(2042..4120)
                     /locus_tag="ACG85N_000350"
     CDS             complement(2042..4120)
                     /locus_tag="ACG85N_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0223151.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(4133..4408)
                     /locus_tag="ACG85N_000351"
     CDS             complement(4133..4408)
                     /locus_tag="ACG85N_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223152.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(4770..5225)
                     /locus_tag="ACG85N_000352"
     CDS             complement(4770..5225)
                     /locus_tag="ACG85N_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223153.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(5379..6587)
                     /locus_tag="ACG85N_000353"
     CDS             complement(5379..6587)
                     /locus_tag="ACG85N_000353"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFQ0223154.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(6699..7031)
                     /locus_tag="ACG85N_000354"
     CDS             complement(6699..7031)
                     /locus_tag="ACG85N_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0223155.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            7179..8054
                     /locus_tag="ACG85N_000355"
     CDS             7179..8054
                     /locus_tag="ACG85N_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0223156.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(8136..8717)
                     /locus_tag="ACG85N_000356"
     CDS             complement(8136..8717)
                     /locus_tag="ACG85N_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFQ0223157.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(8723..9982)
                     /locus_tag="ACG85N_000357"
     CDS             complement(8723..9982)
                     /locus_tag="ACG85N_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0223158.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(9984..11030)
                     /locus_tag="ACG85N_000358"
     CDS             complement(9984..11030)
                     /locus_tag="ACG85N_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223159.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(11265..11540)
                     /locus_tag="ACG85N_000359"
     CDS             complement(11265..11540)
                     /locus_tag="ACG85N_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFQ0223160.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(11783..13093)
                     /gene="der"
                     /locus_tag="ACG85N_000360"
     CDS             complement(11783..13093)
                     /gene="der"
                     /locus_tag="ACG85N_000360"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFQ0223161.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(13275..14504)
                     /gene="rpsA"
                     /locus_tag="ACG85N_000361"
     CDS             complement(13275..14504)
                     /gene="rpsA"
                     /locus_tag="ACG85N_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFQ0223162.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(14639..15319)
                     /gene="cmk"
                     /locus_tag="ACG85N_000362"
     CDS             complement(14639..15319)
                     /gene="cmk"
                     /locus_tag="ACG85N_000362"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFQ0223163.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(15368..16003)
                     /locus_tag="ACG85N_000363"
     CDS             complement(15368..16003)
                     /locus_tag="ACG85N_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFQ0223164.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(16069..17472)
                     /locus_tag="ACG85N_000364"
     CDS             complement(16069..17472)
                     /locus_tag="ACG85N_000364"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFQ0223165.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(17459..18490)
                     /locus_tag="ACG85N_000365"
     CDS             complement(17459..18490)
                     /locus_tag="ACG85N_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0223166.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            18521..18742
                     /locus_tag="ACG85N_000366"
     CDS             18521..18742
                     /locus_tag="ACG85N_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFQ0223167.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(18907..19644)
                     /locus_tag="ACG85N_000367"
     CDS             complement(18907..19644)
                     /locus_tag="ACG85N_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFQ0223168.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(19797..21137)
                     /locus_tag="ACG85N_000368"
     CDS             complement(19797..21137)
                     /locus_tag="ACG85N_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0223169.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(21374..22897)
                     /locus_tag="ACG85N_000369"
     CDS             complement(21374..22897)
                     /locus_tag="ACG85N_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0223170.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(23275..23886)
                     /locus_tag="ACG85N_000370"
     CDS             complement(23275..23886)
                     /locus_tag="ACG85N_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0223171.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(24009..24134)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(24216..24932)
                     /locus_tag="ACG85N_000371"
     CDS             complement(24216..24932)
                     /locus_tag="ACG85N_000371"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0223172.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(24938..25507)
                     /gene="scpB"
                     /locus_tag="ACG85N_000372"
     CDS             complement(24938..25507)
                     /gene="scpB"
                     /locus_tag="ACG85N_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFQ0223173.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(25491..26261)
                     /locus_tag="ACG85N_000373"
     CDS             complement(25491..26261)
                     /locus_tag="ACG85N_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFQ0223174.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(26258..26629)
                     /locus_tag="ACG85N_000374"
     CDS             complement(26258..26629)
                     /locus_tag="ACG85N_000374"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0223175.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(26648..27535)
                     /gene="xerD"
                     /locus_tag="ACG85N_000375"
     CDS             complement(26648..27535)
                     /gene="xerD"
                     /locus_tag="ACG85N_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFQ0223176.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(27631..28104)
                     /locus_tag="ACG85N_000376"
     CDS             complement(27631..28104)
                     /locus_tag="ACG85N_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0223177.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(28209..29072)
                     /locus_tag="ACG85N_000377"
     CDS             complement(28209..29072)
                     /locus_tag="ACG85N_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFQ0223178.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(29268..31367)
                     /locus_tag="ACG85N_000378"
     CDS             complement(29268..31367)
                     /locus_tag="ACG85N_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFQ0223179.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(31587..32696)
                     /gene="rpoD"
                     /locus_tag="ACG85N_000379"
     CDS             complement(31587..32696)
                     /gene="rpoD"
                     /locus_tag="ACG85N_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFQ0223180.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(32720..34561)
                     /gene="dnaG"
                     /locus_tag="ACG85N_000380"
     CDS             complement(32720..34561)
                     /gene="dnaG"
                     /locus_tag="ACG85N_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFQ0223181.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVRNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(34743..37058)
                     /locus_tag="ACG85N_000381"
     CDS             complement(34743..37058)
                     /locus_tag="ACG85N_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0223182.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(37243..38103)
                     /locus_tag="ACG85N_000382"
     CDS             complement(37243..38103)
                     /locus_tag="ACG85N_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFQ0223183.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(38162..38794)
                     /locus_tag="ACG85N_000383"
                     /pseudo
     CDS             complement(38162..38794)
                     /locus_tag="ACG85N_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            38961..39722
                     /locus_tag="ACG85N_000384"
     CDS             38961..39722
                     /locus_tag="ACG85N_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0223184.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            39748..42339
                     /gene="mprF"
                     /locus_tag="ACG85N_000385"
     CDS             39748..42339
                     /gene="mprF"
                     /locus_tag="ACG85N_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0223185.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(42527..43714)
                     /gene="tuf"
                     /locus_tag="ACG85N_000386"
     CDS             complement(42527..43714)
                     /gene="tuf"
                     /locus_tag="ACG85N_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0223186.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            44082..44438
                     /locus_tag="ACG85N_000387"
     CDS             44082..44438
                     /locus_tag="ACG85N_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0223187.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT        12421 a         9412 c         7590 g        15045 t
ORIGIN      
        1 cttctaccca ttcactcaag tattcccacc gtgccatttt ttcttcaagt tgtgcttctg
       61 tttcagatac gtcgttttgc aattcttgta atcgagtgaa attatctcct tgatgattca
      121 tctcttcttg gagcaatgag attttttctt caagttctgc aatctcatct tcaatcgttt
      181 cccattcttt ttgttccatg taagataatt tcttcttttc agcaggttcc ttgctctgta
      241 tttttggttt cgctttttca gcaggctgct gggtctcttt ttgcttcagt aaataactgg
      301 acatgtttcc gtaataaagt tcttgttttc cgtttccttg gaatactaat aacttatctg
      361 ctaccttgtc caagaaataa cggtcatggg aaacagtgat cacagcacct ggaaaacttc
      421 taaaataatc ttccaaaatc gtcaatgttg ctatgtccaa atcattggtc ggttcatcca
      481 ataataaaac attaggttgc tgaatcagca gtttcaacaa aaataaccgg cgtttttctc
      541 cgcctgaaag tttgcctatc agtgtcccat gcataaatct tggaaataaa aatcgttcaa
      601 gtaattctgc tactccgatt tgactgccat ctgctgtttt tgcttcttca gcagcttctt
      661 gtaagtaagc aatcatccgt ttatctggtg ccatttcttc attttcttgt gtataatacg
      721 caagatgaac cgtttcaccg atagacattg taccgctatc caggggaatt ctgccagcta
      781 gaatattaag caatgtcgat tttcccgcac catttttacc agtaatacct agtctttccc
      841 gagattgaat caataaatct aaatgttcta gcagtgtttg gtcatttata cggtaactgc
      901 catctttgat ttccagtact tttttcccta aacgttgtgt cgctaggttc agctctaaat
      961 catcttcttg gtttacttgg tacaagttct cctttagatc ttcgaaacgg ttgatccgtg
     1021 cttgctgctt ggtgccacgt gcctgtacgc cagccctcat ccaagctaat tcctgtttat
     1081 aaagccgctt gcgcttttct tcttgttcta cttctacacg atcacgttct gctttttcta
     1141 agacgtaagc ttcataattt cctttatact cgtataattt tccaaatgaa agttcgaaga
     1201 ttcggtttgc tactctatct agaaaataac gatcgtgggt aaccatcaat agtgcgccac
     1261 ggtattgttt caaatagttt tccaaccact cgatcgcttc ataatctaaa tggtttgttg
     1321 gttcgtctag taatagtaaa tcgggttcat caatcaacac ttgcgccaga ctaactcgtt
     1381 ttttctgacc acctgataaa gtactgattt ttttatctaa ttcactgatt cctaattttt
     1441 gcaaaataat ctttgcgttg gtatctgtag tccaagcatc ttctttattc atttgttctt
     1501 ctgctttagc ataacgtttt tgcacatcag ggtcttctcc gttttcccct agttcgatca
     1561 atgcttcctc atatgctcga acagtttgga tcaaggggct gtttccttga aagactgctt
     1621 ggagaacagt taattcatcg gaaaattcag ctgcttgaga aagataacca atacggtaat
     1681 cagttggata aaacactgtt tgccgatcac cgtcgctgct atcgataccg gcaataatat
     1741 ttaagagact tgttttaccc gtcccgttcg tcccaatcaa cccgatacgg tctttttcgt
     1801 ggaccaaaaa agaaatctgg tcaaacaaat ctttttcacc ataagttttt ttgaggtcag
     1861 ttacttttaa ttctttcaac atgttccctc cttatcttgg atattgtatc ataaaaatag
     1921 tgaacttcag gaaaattttt ttggtatttg ttctttttgt gttctcaaaa aaagaacagg
     1981 accgattcga tcctgttctg atatgttttg ttttttataa aacttgaaaa tttttacctt
     2041 tttattttat tggactcgtt ttttttgact ttgctcgaaa cgcaaatcgg aaagaaaagt
     2101 tatcagatgg tttgttcatc gtataagatt ctaacaaatt gatattcctg cgatagctgt
     2161 tcgcagaaaa cgtcgtgatt ttttcagcaa cttcgtattc atcaatgatt tttctttcaa
     2221 gataataacc tcttagcatt tttttcgtaa acagactatc ttgctggatc atccacgaag
     2281 aaatcaaatt ccccgagttc atttttattc cttcaagttt gcgttgctgg ataaaaaatt
     2341 gaaccatcgt ttcatcataa atatcagtca agttatctaa actttcaata atcaactgag
     2401 tatttttttg atatgtctct ctgatttctt ggatatcttc atcactaata aatgtctgac
     2461 gatcagcata tcttctttgc caaaacattt tcggatggag aacgattcgc attaacctac
     2521 ggacaacaat gaaccaaaag ccgataaaag acaaaatctg gcttgtgata gaatgttcga
     2581 atcgagaaag aaaacgggag taaaaacgat aaccattaga agaaagcttg cccgaacgat
     2641 aactttcatc caatccatcc cgttcgatcg acaaaatcag tgcttgtatt tcttgtactt
     2701 cttgcttttc atcatcggtt aaatcttcca agtacagatc acgtatccgt tcttgataat
     2761 tttctatcac tgcttcagtg gctaaaaatt cttttgtatt acgatcaatt tcctttattt
     2821 cttttcgcaa aacttcaacg acctcttcta aaatcatcaa agcattcaaa tccgtgcttt
     2881 tcgcttcctc tgtttccgtc agcataggca agacaatgat ccctatgacc aaagtcacta
     2941 agatgacacc cgccgtcaga aacaaaagca aagaccgctg gtaaaacatc atattattga
     3001 ttgaaaatgg caaaataaaa atcgtagcca aactgactgt tccctttaca ccgccaaatg
     3061 ttaagatcaa gatctcgttc aactgttttt tgaacttttt actgccatct ttgaatacat
     3121 aaaaaagact gatagaaatg aatctgatca caaaaagcat gacagagatt aatacgataa
     3181 tcgctaataa aagaccgttt ggatataaac cgtctcccca tactggcgag aacacttgcg
     3241 tcaattcgat tcctaaaaat ataaatacta atgcattcag tgtaaaagca atagttgtcc
     3301 atgtactgtg ggacaagctc gacaattctg catcaaacac agagattttc ctaaaaccag
     3361 aagcttggag aatacctgct gccactgcag caatgattcc agatacatca aatgcctctg
     3421 ccaatacata ggctagaaaa ggaagcaaaa gttcgatcag cagataccct gtgacatctt
     3481 gtgctgatgc tttttcgatc aaatgaatta ttttttgttt gaaccagaca agcaaaaagc
     3541 cgatcaaagc tcccccaata ctagaaacta ctagactcat ccccgcgttt actgctgaaa
     3601 aagatccggt gatcagcgca ccaagagcaa actggaaggc agtaacacca gatgcatcat
     3661 tcaaaagacc ttctccttcc aaaatatgca tcgccttttt agggatattc actcgtccag
     3721 aaagagaact gactgctacc gcatctgtcg gacctaacgc tgctccaaaa gcaaagcagg
     3781 cagcaagtgg gacacttggc aacaggaatg ataacgtcgc acctaccccg acaagggtca
     3841 aaatgactcc tccaaatgcc aagaacaaaa ttgtcccgaa atttttgaga atcgaagaaa
     3901 tatctgcttt ttctccttct ctaaataaca atggtgcaat aatcataact aagaacaatt
     3961 caggttcaaa atcgattgat tgtccccact ctgtcagacc taataccact ccaaggaaaa
     4021 tttggatcag tggagctgga atcattggca aaatgcggct agctatgttc gataaggtga
     4081 tcgttactgc aaatacaata actaactcag caaattccat cgtccatccc ctttattttt
     4141 tattttgtgt aagcattttt aaaaattgtt cttttttctt taaaattttg gtttcgacta
     4201 cttcaacctg tgtttggttc ccctctaatt cacactgtac acgttttgac tccaaacgaa
     4261 gcagatcatg tttcagacgt tccaactgac gatacatcaa tatatttctt ttacgcaact
     4321 tacaaagtat ttgacggcgg tcatccatcg aaagggcgcc atagcgatca tgcaacagtt
     4381 ttttttgttg ttcaaaactc tctctcattt tccagatctc ctatacacaa acttccatca
     4441 ttatagcaaa gttctttttg gatgaaaaat atttcattgt ttcatcgttt attgctagtt
     4501 agaaatttcc ataaattttt caacgcctca ttctattcca aatagtatca gatatcaagt
     4561 aaaacttaag aaagataaaa gtttctgaca aaaggatgtt tactttccca ttcctgcctt
     4621 tcttgttctt ttatcacaac aaaaaaatgg ggctgcgaca aaaatcttgt cacagtccct
     4681 tattctgaat aaacggtgtg gaagagacag cctatcagta gtaataagac acatctcttt
     4741 cacagcctca tttttgcgta aaaatcgatt tattcgaaaa attccccgta tacatcttct
     4801 acacgttgat ctggattgat cacgatatac aagttacctg ttttttcgct gatttttgtt
     4861 gattgatatt gattgtctaa tccttctgtt cctttatcta gatatgggaa gtacacaaga
     4921 tcaggtgtgc catttcggta aaggatttcc attcgctcga tatcttcgta tttcaatgcg
     4981 cgattaaaca tgcccatttc cagtccgcct aggttgatat catgtgtcgc aactgcgtcc
     5041 ccttcatgga agatttcgat tttaaatcca gcacacggat ggatttcaac aaattcactg
     5101 ccattgattc gtccaaaact tgttgtgatt tgtttgatcc aaagatcgcc gatatgacgg
     5161 cggttgatcg tccaggtttc tccatttcta aaataaaatt ttagcgctgt catttcttta
     5221 ctcattaaaa agactccttt atgatgtaat ttattcttgt actacctcag tttagcacaa
     5281 atttatgtaa gtggtcacat cttatgtttt ttcttctaat gacgtatctg cccactttaa
     5341 gtagccaaca aagctgagca gatacagtct agtgtttttt aaatcaattt ttctttcgat
     5401 aaaaaatcca aaacctgttt tttatcattt tttattcttt gttctacgac ggctttttca
     5461 gctaattgca aagcttcact aacccactta ccaggttttt ttccagtttc ttctagcaag
     5521 attttaccat ctactgctaa ttcgcttaat gacttgatag gtaaacgatc ataggattct
     5581 cctacggcat ccatctgagg tgtttcgtca aaatagatca tcaattcttc tacttgcagt
     5641 gcatgttttt tgcctaagaa gtacaattcg aatggttcca aagctccagt ttctcgttgt
     5701 cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt ggttagaaca cttccacgat
     5761 ttcaaaaagg aacgaatctc attttctttt agatccagtt ggctgatcaa taatgcccat
     5821 gcttggctct cttctttcaa tgggacggat ggtaattcag caaaacgtaa aagtgcttcg
     5881 ccttcttctt tcaaaccggg acagcaaatg tagcactctg tttcaataaa tgcatggatt
     5941 cccgcggccc gttcatttcc caagagcatc ttgatgaatt ccacatttat acgttctaca
     6001 gatattttac ctaatagctc gtggttttct ttgatggcgt agaaggtttc ttcctctaat
     6061 ttgaacccta gttgactgac aaaacgcaac ccgcgcatca ttcgtaaggc atcttcatgg
     6121 aaccgctcat gaggattgcc tacagctcgc aagatctgtt cttctagatc ttgcagacca
     6181 tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa acgcattgat cgtaaaatct
     6241 cttcttttca aatcttctgc caacgaacga acgaattcga catgatctgg tctacggtag
     6301 tcctgataag ttgattccgt ccgaaatgtg gtgatctcat attgttcatc attttctaat
     6361 accaacacag ttccatgttc gatcccaata tctatcgttt ttggaaaaag ctgtttgatt
     6421 tcttctggaa atgcgcttgt tgcgatatcc acatcatgga ttggctggtt cagcaagata
     6481 tcacgaacac ttccacctac aaaataagct tcatatcctg cattctctaa ttttttcaga
     6541 actggaattg ccttttggta ttccagaggt aattgtttta atttcatttt tccaaccttt
     6601 cctgcgtgtt tcttgtcctg agataaagac tcagttgcat ggtgactgtt tgacaagtcc
     6661 gtttcagagt cttcaaggcc aggaccactt catcttgttt actcgtcctt acgctcaaag
     6721 cgattatggt ctctttgatt gaatttttcc atgttttttt caaaaacttc agttaagtcg
     6781 atattcaatg aattagccat gatcatcgta acgaataaaa catcaccaag ttcttctgct
     6841 accgagttaa ctggttcaga agcctttttg cttttttctc cataagtgtg gttgacttct
     6901 cttgccaatt cacctacttc ttcagttaat cgagccatct gagctaatgg agagaaatac
     6961 cccaccttga attgctggat atattcatcc acttcctgct gcatactgta taaagaacgc
     7021 tttttatcca tctttttccc tccaaaacta tcttaccaaa actttaacgc aaatcctaga
     7081 taagagcaat tgagaaaatc gaatttccat gctacattta ggaaggaatt tctaaatgga
     7141 aattctgtga aaatacataa ttgaaagctg gaattcatat ggtcaaacga aaaacagtaa
     7201 aagaagttct ttttatcatt ataggaacaa gtatttatgc atttggtctt gtttacttga
     7261 acatcgctaa tcatttagca gaagggggag tctcagggat caccttgatc ttaagagctt
     7321 tatttggtat tgatccagct tatacgactt tattaatcaa catcccattg attttactag
     7381 gaggaaagat cctcggtaaa cgttccttgg cctatactgt tctaggtaca ctttctttgt
     7441 cattcttttt atggatctgg caaagggttc cactagaaat caatttacag catgatctac
     7501 tgatcgtttc tctacttgcg ggtttagttg caggtgtagg aagcgggatc gtctaccgca
     7561 tgggtggaac aacaggcggc agtgatatta tcgctcggat tttagaacaa aattacggca
     7621 tcactatggg gcgttcccta ttggcatttg atatcatcgt cttacttgct tcattaacct
     7681 atatcgactt gaaacgtatg atgtatacgt tgattgtttc gtatgttttc agtcgtgtga
     7741 ttgattcgat cttagatggc ggatattctg caaaaggtct acttgtcgtc tccaacaaaa
     7801 gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag aggtgtgact ttcctttctg
     7861 gagaaggcgc attctctggt aattcaaaaa aaatcatcta tatggtcgtt agctctagtg
     7921 aattaactga agctaagagg atcgtccacg agattgacga aaaagctttc ctttcaatca
     7981 tcaatgtgca tgaagtggaa gggcaaggct ttacttattt gaagccacag acaagattac
     8041 tgaaaaaact ataagaaaaa agacaaagat aggctatgag gaaagattcc acatccagct
     8101 gacctttgtc ttttttagta ctatactatt tttaactaat tgttatgttc acttacccgg
     8161 ttacttttct tttgtgtctg caaaaggatc tttcggttaa cttcatcaga taatttcaaa
     8221 aaagcatccc tatctccatc ttctagtgct tgatcgattt gagcataaag catttttagt
     8281 tttgcctctt cttctttttc ttgaaaatat cgttcaatta attcagtatc ttcttcactg
     8341 actgaagaat cccaagaaga aaatggattg tcttctaaga tagatagata ttctcttgtc
     8401 tgccaagcat tttcgaatat acattccaca tataaaggct ttttccaatt caaacggatt
     8461 tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct gcaaaaacaa tttcatgggt
     8521 tccccttcaa cagatagatc actgatctgc agtcctcgat ccgcaaaatt cgccccttca
     8581 acaaagtgga cattgcttaa aatcgcttca tgattggcaa gataattcaa gatccaaaaa
     8641 acttctcttc ggctaaaaga tacatgattg accatccacg ttaaaaaatt cttcttttcc
     8701 cgtacatcaa ttagcatttt tctcacctct ctgttaggtc atctaataat gacagtgcct
     8761 ccatatctcc tggttcaagt gttaaataat aagtcaataa ttcttgcgct cttttcagct
     8821 gcccttcttc tcttaagaaa aaggcatact ctttcaagaa atctggttca tgtgatagtt
     8881 catgataagc ctgttcatag tgtacagctg caacggtaaa gtcttctaat tcattataag
     8941 catgggctag gttccattct gcataaggat tagcagtttc ttccatctga ttgatcactt
     9001 gtatcacatc ttcatatctt tcatcatcca agtaaagatt acttaaagta agtaatgttt
     9061 catcttgttt gtctcctact tctaaagctt tcagtaaaag ttcttctgct cgctgtctat
     9121 catgcagacg gaaagcattt tccgaggcca ggtgataaag ttcgacttgg taaggatttt
     9181 ctttgattcc ttcttctaac actgtttgtg cttcttcaat catttcttct tcttgaagag
     9241 cttgtcctag atatagatat aacgattgat aatgcggatt aacttctcga agttcttcca
     9301 gatagttgat tgcctgctga ttttctttca attgaagatt cgtaaaagct agctggaaca
     9361 gtcgatcatc tgtcctttct tctttcagtg cttcttctaa aggtacgata gctgcttcga
     9421 attctccttg catacttaag gattgtccta aacgttcctg catcgagatt cctgaaattt
     9481 catttatccc gcttgccaac aatgtttcat aaaccactgc tgcctctttg aaacgatcaa
     9541 ctgaaaaata caattcagct agcgcaaact ggatcaatgg ttcatcagga agcaacccag
     9601 ctgcttcttt caattttgct tcactcactt cagggattcc gataacttgg tacaaatcag
     9661 ctatcgccaa taagctttgc ggatagtaat cactttgctt atccactttt tccaggtatt
     9721 caaaagctaa gtcgatctca ttatcttcga ttgcaatttc tgctaacggg atgttcagtc
     9781 cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat ctgttttgct tcgatcaaaa
     9841 agccaatagc cagcaattct tcgcctagtt cagctaatat atctgctggg tcttctttta
     9901 atgctttttc taaaaagatt tgtgcttgtg acaagtcttc atctgataaa gcttgcaaca
     9961 ttttttcgct atttgtattc atttcaattc cctcttatct cactgacgat ttgttgaaat
    10021 accgtcattg ttctcaataa ttctttttcg tttggatgtt ggtatccgtc tattatacct
    10081 attttttcta aaacaagtat ttttttgctt ttttctgcca aaaaaagaac tttttccaaa
    10141 tactccatct ttgaaatctg ttccagccag tggattggat agccaagcaa gtccagccac
    10201 acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt ggagattcca cacgaaatga
    10261 aacatcattc ctaaaaattt tttcatacta gcagaaagta gatggccatt ttcggtcaga
    10321 taaaaagctt gttcaaacag tttaccataa tttgacaggt tttctgcctc taattgataa
    10381 aaatagatca tttcttcgat catcccggca aacggccgag tcgttaattg ctttttattt
    10441 ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac aaatcattcc gcaacagata
    10501 aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct cgctgattgt ttgatcataa
    10561 acgatgcgtt ctggcagatt gatggtttgc agcactacac gatttggtag ctgcaaaatc
    10621 gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg atacagggat catccatagt
    10681 gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca gatccatgat cccttcattt
    10741 ccaaatccga tgataaacat ggattgattt tcgggatagc gtttgacgaa atcaagcagg
    10801 ttctttaatt ctgttaaatg attgcattga gtgttggcac aaatatacca gtcattgttt
    10861 gcttgatttg caaataacgg gttgatctta tctgcaaaca aatcataata gcgctgattc
    10921 gttaaaaata agattggctg atcaatggaa agttctctta gctgactgac tagcgtttct
    10981 ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat caagttccat acgcatccct
    11041 cctcttctta tttttatttt accattagta agcaaattct agaaaagata agagcaaagc
    11101 tcacctcata acttgcaaat aaataaaaat taaataaaat acatgtgttt tctatgtatg
    11161 acatacggat tcggaatctc tgtctcttat ttattccttt tctgctagga aataaaaaga
    11221 aaagcacttg tagccctaga gccacaagcg ctttttcaac tgtcttattt aacagcgtct
    11281 tttaatgctt tacctggttt gaatgcaggt actttgctag ctggaatttc gatttcttca
    11341 ccagtttgtg ggttacgacc tttgcgagct gcgcgttcgc gaacttcaaa gttaccaaaa
    11401 ccgattaatt gaactttttc gccgttagct aaagcatctt gaatagttga aaatacagca
    11461 tctactgctg cagttgcatc ttttttagtt aaatcagtag ctgaagcaac tttttcgatt
    11521 aattctgctt tgtttgccat ggatagattc acctcctcca caagagatat gatagaaata
    11581 tcatttagtt aaaactttct gagtggtcca gaagtttaaa aagaaatatt gattaacttc
    11641 aatattctgt tatcacgata tcatatgaac cgcttaatag caaggttttt accaattttc
    11701 acatgctttt tttagtcata gaaacgtaac aaacgaaact gtctttttct tttgaaaaaa
    11761 aaacgtcgat atggtaaaat gcctattttc tcctacgagg gataattcgg atggacgtcc
    11821 cttcaaaagt aaatgctttt ctgatttgat tttctaagaa acgttcataa gaaaaatgca
    11881 tcaattcctc ttcattgaca aaaacgacaa atgtaggtgg tttgacagcg acttgtgtag
    11941 cataaaaaat cttcaggcgt tttcctttat ctgttggtgt tggattgatt gctacagcat
    12001 ccataatgat atcattcaac acagccgaag agatacgaag attctggttc atacttactt
    12061 gttcgatcat ttcaggcaga cgttccaacc gttgtttagt cactgcagaa acaaaaacga
    12121 ttggagcgta atcaagatac cggaattcat cacgaatctc ttgttcgaaa tcacgcattg
    12181 tattggtttc tttctttaca agatcccatt tattcacgac gataataatt ccacgaccag
    12241 cttcatgggc gtaacccgct actcgcttgt cttgttcccg aatcccttct tcagcattca
    12301 gtaccatcaa aacgatatca gaacggtcaa tggcacgcat tgcacgcatc acgctgtatt
    12361 tttcagtatt ttcgtatact tttccgcgtt tacgcatacc agctgtatct atcattaaga
    12421 atttctgacc actttcagaa acgaagtggg tgtcgattgc gtctcgggtc gttccttcaa
    12481 tatccgaaac aattacacgt tcttctccta aaatcgcatt gatcaaagag gatttaccaa
    12541 catttgggcg accaatcaaa ctaaacttaa tcgtgtcgtc gtcctcttct tcagtctctt
    12601 cagagaaata cttcactgct tcatctaaaa tatcaccaat ccctagtcca tggcttcctg
    12661 aaacaggata aggatcgcct agccctaagg aataaaattc atagatatcg tttctcattt
    12721 ctggattatc tactttattg actgctaaaa tcactggttt attacttctg tataaaattt
    12781 tcgcaaccat ctcatctgcg tctgtcacgc cttcgcgtcc gctgactaca caaatgatta
    12841 catctgcttc ttcaatcgcg atttctgctt gatgcttgat ctgatccata aaaggttcat
    12901 cgccaagatc aattccgcct gtgtcgatca cgctgaattc tcgacctaac cattcacctt
    12961 ttgcataaat acgatcacga gttactccag gagtatcttc gacaatcgag atacgctcac
    13021 cagcgatgcg gttaaaaatc gtagacttac cgacgttcgg gcggccaacg attgcaattg
    13081 ttggatttgc catattattt cctcctcaca agtttccaat ctcacttagt ttaacgaatg
    13141 cattgccaac tttcaagcat agattgtcaa ttaaacgaag tatttttatt ccttattgaa
    13201 atactaatcg cttttcaaaa gaaaaactga gacaaaagca gctgctttca tctcagtcac
    13261 agatacaaaa tcaattattt ttcagaatca tcggttgctt tttcttctac ttcttctttc
    13321 aacgcatcac ctaaaatgtc tcccatagta aatccagtat tttcttctgg taattcataa
    13381 gactcaacat tttttggttc ttcttctgaa gctggttttt cttctaaagc tttgatactc
    13441 aatgcaatac gatgttcttc tggatggatc tctaatactt tcactttcac ttcgtcacct
    13501 tcatgaagca cttcatgtgg tgtcgcaata tgtttatgtg aaatttgaga gatatggact
    13561 agcccttcta ctccagggaa cacttcaaca aaggcaccga agcttgtcaa acgtttaact
    13621 gttccgtcta atactgtgcc aactggagct tttgtatcga tttctgacca aggtccaggc
    13681 tgtgtgtctt tgattgacaa ggaaacacgt tcttgttcag gattgataga caacactttg
    13741 acatttacat catcattgac agccaataca tcacttggtt ttgctacatg actatgtgaa
    13801 atctctgaga catggactag accgtcaatt ccgcctaggt cgacaaatgc gccaaaatca
    13861 gtaagacgtg caacacgtcc actgagaaca tctccctcat ggatagttga aagaatttct
    13921 tttttctttt cttctctttc gttttccact accgctttat gcgataagat caaacgattt
    13981 tctgatggtt cgatttcgat gattttaaag ctcaatgttt tgcctttata gtctgaaaag
    14041 tctgcaacaa aatgatcttc caccattgat gcagggacaa aaccacgtac accgacgtca
    14101 acgactaaac cgcctttgac aacatttgta acaggtgctt cgatgacctt gccctcttgg
    14161 aaatctcttt cgatatcttc ccaaactttt ttagcatcta aacggcgttt tgaaagtaga
    14221 tagctgccgt tttctttatc tttgccgatc gtactgataa cgactaaatc taatacatca
    14281 ccgactttta ctacttcgct gatatcttct accggcaaag ttgataattc ttttgccgga
    14341 acgactcctt caactccagc cccttcgatt ccgacaatca cttgtttgtc ttcaatgact
    14401 aaaacttcgc ccttaacaac atcgccaact tttacttctt gtacgctttt cattgcgtct
    14461 tccattgact ggttgttctc ttgattttga tcaaattctg tcataacact tgtcctccta
    14521 accaacattc tgccgtaagt acggtactgt ttttagttta attgattatt caaaaaaaat
    14581 aaagcgattt cgttcgtttt ccttgaaaaa acaattgttt ttcatttttt ttcttaattt
    14641 aaaaaagaag ataacctttt ttcaaaacaa cagcttttat tgctgcaacg acttctggaa
    14701 tcgatttgcc agtcgtatcg atacgaacag catcctctgc ttgcttcagc ggagagacgt
    14761 cccttgttga atcataatgg tctctttcct gtatctcttt cgtcagtgtt tcaagatcag
    14821 tcggaattcc tttttcttgg ttctctttga aacgtctttc tgcacgctca cttacgcttg
    14881 ctactaaaaa gattttcact tctgcattcg gtaatacagc tgtcccgatg tctcttccgt
    14941 ccatgacaac gccaccgcgt tcccctattt ttcgttggac agcaactaat tcttctctga
    15001 cttttccatg tgcagagact tcagaaacag cttttgtgac atctggttga cggatttcta
    15061 aagtcacatc ttttgatgct atgaatacat gctgtccatc ttcttgttgc gaaaaagaga
    15121 taggatactt tttgatcagc gaaactagcc cttcttcatc tgaaaaagat acattgttcg
    15181 taatagctaa ataagttact gcgcgataca tggcaccggt atctgtataa atataattca
    15241 attcttttgc taatatcttg gcaacggtac ttttcccaga tgaagcaggc ccgtcgattg
    15301 caatactgat ttgtgtcatg atataactcc tttttctcag aaaaaaagcc gcaaagcgcg
    15361 gctattatta tttaattctt aattcttgac cagggtaaag cataaagttg tttggatcta
    15421 agccattcaa ttcaaacaat tgatcagtgg tgatccctgc acgctcagcc acttgttttg
    15481 gaccttcgcc tgcttgaaca gtcgttgttg aaccggctgc agctgatgaa gaaggtgttg
    15541 tttctgctgc attgctgctt gaatcccctg cttgataagt tgcctcactt gaggaagtcg
    15601 gtgttgtctc tgctgcaata ctgctttctg acgttacacc tgtatcttga gacacagtag
    15661 attcttccgc gattgtacta gattctactg ttgaagtctc agttgactcc tcttttgttg
    15721 tacttgtaga actttctact actgatgtcg aagtttcaga agaagttgct gctgttgcat
    15781 tgttgcttga tccatttctg atccagaaat aagcacctgc tggaatagcg atacataaga
    15841 ccagtaaaat caaaaatagc gaaagaaata atgtatttcc tttcttttgt tgtctctgag
    15901 aacttcttga tgaaacttca tcatctgtat cgtagattgg ttgttcccat ggttcttgtg
    15961 tttcgttatt ttctgtagat tgatgtctat cttttctgct cactgtttta cctcctaaac
    16021 tctatcgtgt gtattatacc ataggaaaaa gtgtcttgtc ctaaaaaatc aatgttccat
    16081 gtttttttta aataaattta gtaaaatcgt tccccaatga agttgttcag tcttagaaaa
    16141 aagcttcctc tttggcagaa gttctgcttt tgctccatca atatcacaac aattctgcgg
    16201 cttttcggag agtttttcgc caaaataagc taagatgaat tttctgcggc agttttcttc
    16261 attaatatag cgaagcatct gctgcaggcg gaattctttt tctttttcct gacgtttgag
    16321 ccgttccacc caattttctg gtttttcaga ctgctggatc agctccatcc attttttttg
    16381 cagctcatca aagggagctt gctctgtggc atattccaaa taaagctcaa aactttgtcg
    16441 ctgttctcta gaaagttgat taaagaaata atggatacgt tcatccccac tttgataaaa
    16501 aagaattgct gcactttctt cctgatctct gcctgctcga ccgatttctt gtacatagtt
    16561 ttctagactg tctggcaaat cataatggac cacataacgg atatcagatt tatcgatccc
    16621 cataccaaat gcattcgtag caatgagaaa ctgcagctga ttttttacaa actgctgttg
    16681 aagttgtctt ctttgagctg catcaagacc accgtgatag tatccaactg taaaacgttc
    16741 cctgaataaa tgatacaatc gttcgacttc tttttttgtt gcacaataaa tgattgccgc
    16801 tccttgtgct ctttccatga agtgttcaag ttcttgttct ttttgctgcg ttttccgaac
    16861 aaaaagcgag atattttgcc gattgactga ttgtctgatc tcttttggtg gtcgatcaaa
    16921 tagtacatga cgtatatctc ttgctactaa atctgttgct gtcgccgtta gtgccagtgt
    16981 gaccgggttt cctaaaagct ttcggatttt tcctaactgt tgatattctg gacggaaatc
    17041 aactccccat tgagacacgc aatgagcctc atctactaca tataacgcaa tattttgttt
    17101 tttcagatgc tctaaaagag aaggattagt caacatttcg ggactcaaaa acaaaaattt
    17161 atattgcgat agatgacgga ggacgtatct tctttcagat tgcgaaagta aactattgaa
    17221 agcagctact cgtttctccc ctcgtttttg taattgcgtc acttgatctt ccattagaga
    17281 aagcagcggt gacacgatga tgaccagacc ttccattaaa taccccgtca attgatagca
    17341 aaggcttttt cccgttcctg taggcaaaat agcgagagta tcttctccat caagcaacgc
    17401 ctgaataatt tcttcttggc cgggacgaaa tgaatcaaat ccaaaatatt tttttaattc
    17461 atttattaac attctgtatc gcttcctttc tcaatagata gatttgatac aaacgaaatt
    17521 ctccataatc taattgtcca tctacattcc attcgctaaa gcgccactct cgcacggaat
    17581 ccttatttgg ttccaaacgt tcgattgttt ctgcagagag aatccactcg tatggaaaat
    17641 catcaaaaaa caaggcccac tcaataagat gatctgttac tgttcctctt tttaaatgac
    17701 ggattgccat gatctggtca attgtctgac cattcataaa catctgcttg gtgatcatca
    17761 tgctttgatt gaaattttgc tgtaataatg aatcgatcaa agcgtgccaa agcgaatctg
    17821 gccgtttttc gatttgtgct aacaaaaggt ggatacatct gctttgttgc agataaaccg
    17881 ctgactcatc attgtttttt gccagctgat aaggaagcag ccctgttcgc tcatttccag
    17941 ataattggtt agctaaaaaa tctgctgctt cttctggtat ctccgaaaac agctgcacca
    18001 aactctcata cgcagtatca atcaaaattt cacgtggcaa ttggctttca ctaagccatt
    18061 tttttagttg gaaagtataa aaaggacttg tctctgctgg aagatagtct tgaatacccg
    18121 ttgccagatt agaaattact tgaacagcaa atttaatgag ccgccaacta gtttcccctg
    18181 ttctgccata gcggtcaaaa cgcaaaccag tatgttctat ttgatgttcg gacaacatat
    18241 cagcccctgc aggcgttagc tttgcttcat tttcatttat ttcgatccaa ccttgctgcg
    18301 caattttttg caaaatttga taaaacttat cctgcttcaa ttctggaaaa ctgttatgga
    18361 taaacaaaag ttcgtgggta aatccataaa taagtactga agtcgttcgt tttcccacaa
    18421 ggagttggta aagagaacta atccgcagct tgtgtcctga tgaaaatagc gataaaataa
    18481 aaacaatcat attattctcc ttaagttagg atggttagaa atgtcactac tatgtaaact
    18541 tgtacctgaa cgctgtattg catgcggtct ttgtcaaatc gaagcgccag atattttcga
    18601 ttatgatgaa gaaggaatcg ttttatttgc ccaagaacca gaggctcatc aacagttcgt
    18661 gatgccggaa gaagaaacat atgtaaaaaa agcgtatcaa aaatgtccag ttcgagcgat
    18721 tttattagaa aataaaatat aaatataaac gaaactttaa aaacgtacac taagttctct
    18781 aaacttgaaa ataagccggg atctgtttct cttgttccgt gaaatcccga cttattttga
    18841 aagattcatt tgctatctat agttaattga ctagtttttt cacggaccct ataggaccca
    18901 tttatattat ttcaactttt tcagccagtt ttccaatgta ttgacgatca cagtttgctg
    18961 gtctgcgtta gaaatttctg cctctccatc tcctttttgc tcaccatagc tgccaaaacc
    19021 tccatgattt ccgccattga ttgatacata ctctgttctt tccggtaaat actgcttagc
    19081 ttcttgataa gcttcctgat tcaatacttt atcttttgtc gccgtgatcg ataaaacagg
    19141 aacagctgat tctgtcaatg ttcctttttt gtcgggataa cttgccatga agaatacccc
    19201 ttttaaatgt ttgtctgaat gattcttatt gacataacga cttgccatta cgccacctaa
    19261 tgaatggcca ccgatcacat aatcggaatt tggaaaatct tctttgacct tatccgcttt
    19321 gtttccgccc atcactgcta gatcaagtgg cattttgaca atagcgactg aaaagccgtt
    19381 ttttgctaaa tcttgcgccc aaatactata actttctggc tctacgagag caccaggata
    19441 aaaaataagt aatggagact ttccatgatt ctttttctct ccttcaaaaa ataagtaatc
    19501 ttctttgttt tttccttctt ttaccaacac ttgcgcttct gacgatggtt cgtacgtatt
    19561 ttgctgtata tagtaataac cggctccacc gagtaatcct aaaatgatga ctaagccaat
    19621 tagtattcgt ctaaatattt tcactttctc ccaccttttc tatctgagtg accccttttt
    19681 cttaatcaag tatgattata ccttaaaaaa ataagagaga aatgcaaact ttccagcatc
    19741 aaataaaaaa agacggaata tccatttgaa tatccgccct ttttgtatgt ttatttttat
    19801 ttagcacata aacgatacac agcttctgca taaatttcca ttgctttata catactactt
    19861 aattcccatc gttcattcgc ctggtgcata aagtcaggag tatcagggaa catcgcaccg
    19921 aatgccacac actggttcat cgtacgagca aaagtagcgc cgcctgatac aaacggttca
    19981 gtcatgtctc cagtaatatc gcgatatgtc cccaatagat ttttcactaa ctcactgtct
    20041 agtggcacgt ataatgaatc aaggaagtca aattcttcat aagtcaaccc atatttttct
    20101 gctgttttag ttaatttagc tactaaatca tctttcttga atgtaacagg gattctcata
    20161 tctacaccga tttttgtttg ttctggtgtg atttccaaac ttgcaaagtt catcgttagc
    20221 tcgccggatt gttcatcttc tgttttgccc acgacagctt caccagttgc attttcttga
    20281 actaattttc ctaagaaatt gattgggcca aaatcaaata cttctgaaag agcaattgcc
    20341 agacgagaaa tagcattcac tccttgtgct gcatctttcg cgtggatact tttaccaaga
    20401 acgacgatgc cttcaccttg ttcttcgaaa tcgaattcgt gttttttcaa tgcttctttt
    20461 acttcagaaa gcttttctcc agagtaagga gctgcatcag gaacaacatt caacgctcct
    20521 ccagctttta cagaaaactc gttcgttcct ggtcctgtca agtaagcttg caataagcct
    20581 ttttctgcat aaattaaagg aaattcagca tctggcgcaa atccttgcgt aattccttct
    20641 tctttttcgt tatatttttc taaacaacgc cataaatttt cttcatctgt accaaagata
    20701 aaacgaatac gtgtcttgaa ttccacgcca gcatcaatca aagctttgac ggcatacatt
    20761 gccgcaatag aaggaccttt gtcatcttgg gatccgcgac caactaacca gccgtctttt
    20821 atagttggat cgaatggttt tgtttcccaa ttattttcgt cccctgctgg aacaacgtcc
    20881 atatgacaca agataccgaa taattcatct ccagagccaa tctctgaata accgtaatag
    20941 ccttctggat ctttaaatgt tctaaatcct agcttttcac tgatttccaa tactttatcc
    21001 aaagcttcaa tcactttttt accgaaggga tgtccttgtc ctgaatctgc ttcatctagt
    21061 acggaaggaa ttcggatcaa ttcgctcaat gattctaatg cttgttcatg atgttgttct
    21121 gtcacaaatt ttttcatgat attatcctcc taatatgtta aaaagggtca tgtcaaattc
    21181 ctccgacaca acctcttctt ctcaaataat gacgttttaa gcctgctcca tcagcaagca
    21241 gaatgttctt aacaaatgat gaaagaaaaa acactcactg aatggagcta ttgacttatc
    21301 cacgtgctta tcgatagccg tttatcggtt tcttcattct tatgatagac cttgtacctg
    21361 tatttgtcta ggtttagaac attgctgcta cacctaggat cacacagatg gctacaaata
    21421 atatcgcaat taatttaccg gtaaatttcc accacactga aacatcgaaa cgagcaattg
    21481 ctacagcacc cattacgata gcagatgttg gtgtaattaa gtttactaaa ccagatgcag
    21541 attgataagc agtgattact aaatccttac cgacgccggc aaattcaccc ataggtccca
    21601 tgattcccat tgttgcagca gctaagccgg aagttgaagg aatcaagaat gacataggga
    21661 tatagaaaat aaatgtaata atgatgaata cacttgaaga taatgaactt aatccttgtt
    21721 cgccccaatg taagatggtg tctgtgatta atccattgtt cataaccact tggataccac
    21781 gagcaacagc tactacgatt gctacaccga tcaaatcaga agcaccagct aaaaattcag
    21841 agacaaattc agactctttc atgccataag tgattgctac tacgatggac attactaaga
    21901 ataacatagt aatttctggg aagtaccagt cacctaatgg aagcatgctt ttacctagaa
    21961 ccgtacctag taccggaagg tttgttaacc agtttgtcaa tgattcaaaa atcgtgaaat
    22021 tcgcattcaa ggttgtccaa ggaatcaaac tgatgatcat gataccaaaa gtaataaaga
    22081 acaatacatt aacatgtttt tgttttttcg tgattacttc ttgacgaccg attgcttcaa
    22141 tatcgaaatg cttcatatct tctttacgtt ggttgtatac taatgatttt gttggatctt
    22201 tttcgatctt tgatgcataa cgatacacat atacaataga aatagctaac aaaataacga
    22261 acatcaagaa tcgaagacca ataccttcac ccatcgaaat cccaacagct tgtgaagcta
    22321 cgcctgttgc aaaaggattt accgttgaag ctagacagcc tacttgagac cctactaata
    22381 cgattgctac agctactact gtatcaaagc ctacagccat catgactggg atcagtaaag
    22441 catagaatgg aatcgtttct tctgccattc cgtaagtaga gccgcctaat gcaaataaca
    22501 gcatcaatac tggaataagc attttttctt ttcctttgaa tttcttcaca actgaagcaa
    22561 tcccggcatc tagtgcccct gttttgttga taatacctaa aaatccacca acaaccaaaa
    22621 tgaagaaaga gattgggatt gcagcaggtg tttctttagt tagctccccc gctggtacac
    22681 ctagcatccc attgattggc gccatgaaaa tatcccacag cccttgagga ttttgttgta
    22741 ctcgttcata tgatccagcg acgatgttgc ctgcatcgtc tacactatag tgcccagctg
    22801 gtataaacca agtaagtaca gcgattactg caataataat aaataagacc gtatacgctg
    22861 aaggcatttg aaattttttc ttttttgttt gttccatctt cttcttcctt tcttttccgt
    22921 tagagacaac aattttggct ctaatgttat tttatcaaaa agagtatcag aacaaaaata
    22981 ataacacgca ttttgtaaaa cttttgttca aaaagttcgt ttttccctat tccctcctct
    23041 tttttcaggt ttttaaagct ttcttctttt tcattgtagc gtttcctttt ttttataaac
    23101 aaacattttc gtcttttaaa aggagaagaa tttcacattt gcaaagaatt gattgatttt
    23161 tatgcataaa tcaaaaaaat actcaaaaca aacaaataaa acgtttacat tattgcatgt
    23221 cttgtatttt tttccaaaaa aaaagaccaa gaaatttctt gatctttttg atatttattt
    23281 gactgctgta tgacgttgtt cgaactgatg ttgttttcga cttaaccatg gaagaagttg
    23341 cgcatgcagc actaaaaaga cagcactcac gatgaatcct ttgatcaagt tgaaaggaac
    23401 gatcccaatc agtacatagt ttgctaaaga catgcccagc atctgtgttg cgttcagccc
    23461 taaaaacatt aagtaaagag gcgtaataac gaaataattt gcgatactca taaaaatagt
    23521 tagagcgatg gttcctgtaa tgacacctaa aaatttgttt cttcctttat gctgtttctt
    23581 tacactaaag aaataataaa tcgggaatgt aaagatcgtt gttgataaga aactagcagt
    23641 gtctcctacc aaattatccg gagaaaaacc tgtagtcagc agatgtaata ctgaacgcaa
    23701 gaacgctgtt gcaactccgg ccgctggacc gaataagaac atgctgatca ataccgagat
    23761 atcactaaaa tcgatcttca aaaaactaaa agccggcatg atcggaaaag caaaatactg
    23821 taaaactaat gcgatcgcag caaacatcgc tactgcaacc attttctgta cacgggtgtt
    23881 cttcatttga aatcctcctg ttaggactcc tcttcagcga actttttgac gtttctctcc
    23941 aaatgaaaaa actctatttt ccagggaaaa tagagttaga gttatttgat caagcgtcgt
    24001 caaataagct ctatcttctt tatccagact atactgtcgg tatcggaatt tcaccgattc
    24061 agctattttt ttgcaaaata ggtcgtggac tataaccacc ggtcgggaat ttcaccctgc
    24121 ccctgaagac gaaccttata taatttttgt ttacatattc attatacata tctattcaaa
    24181 aaacgcaaca tctaacactt ataaaaagta agtgttcatt ttgagtggtt cagcaaactg
    24241 ctgacttctt ttttgttcag ttcacggtaa tccccaggac gtaggccttg taaagtcaga
    24301 tctccgtatt tttcacgttt tagtttttgc acaggaagac caactgcttg aagcatattt
    24361 ttcacttgat gattacgtcc ttcgtggatc gtcaattcca cgatactgtg gtttgtctta
    24421 ggatccactg ataaaatttg gaatttagct ggagatgttt ttcgtccttc gatctttatt
    24481 ccttttgtca aaggtgcaag catcgatttt gtagcgatcc cttttacctt tgcaacgtaa
    24541 actttatcca cttcatgttt tggatgtgtc aggcgctgag caaaatctcc atcatttgtc
    24601 agtagcaaga tccccgaagt atcgtaatcc agtcgaccta caggatagat acgttctttt
    24661 accatcggca aaagatccgt aacaactttt cttcctttgt catctgcaac agcagaaata
    24721 actcctttag gtttgtacaa taaatagtaa ccgtattctt cttgataaat tgggacttca
    24781 tccacttcca cagtgtcatg tttcccaact tttatcccta attcccttac aacttgtccg
    24841 tttactttta cacgtcctgc aaggatgaat tcttctgctt ttcgacgaga agtgatgcca
    24901 gcgtgggcaa ttactttctg caatctctcc attacaatta ttctccttta ttcattccat
    24961 caaagaatag atctggtgac acgtcttctt caagtgaaat ttccatttct tgtgtatcag
    25021 gcagttcttc taatgatttc aaaccaaaat aatctaagaa gtagccagtt gttccatata
    25081 atatcggccg acctggtcct tctactctac ctttttcttc aatcagttta tgggcagcta
    25141 gacgctgtac cgctcctgaa acctgaactc ctcgaatctg ctcgatttca gctcttgtga
    25201 ttggctgttt ataagcgatg attgctaagg tttcgacggc ggcttgagat aatgtgtttg
    25261 atgtcccttg cgcatatcgc ttaagtaagg gagccagctc ttttttagtc gttaatacat
    25321 aatggctttc cgtttcaaaa atatgcaaag ctgagtgcga gttttgagtg taagattctt
    25381 ttaattcttt cactaaactt gtgacttctg cttcgctgag atttaacagt ccgcttaatt
    25441 cttcattact gatcccttcc tcgccaacaa taaaaagaag ggcttcaagt tcacttaatt
    25501 tcgtcatgtt cggtatctcc tactgctgga taaagcataa ttgattcata gttttctttt
    25561 tgttgcgcaa tgatctgccc ttttttcatc aattctagca aagccataaa agtagtgaca
    25621 acttcacttt tagaaggtga ctcaaaaaaa gaatcgaaag tacagccatg cgcatttctt
    25681 tttatacgct cttcgataaa taccattcgt tcttcgatcg ttgtgtcatc gggcgtgatc
    25741 gttttttcca ctttttgaga aaatttctta cgttctaaga ttttctgcat ggcacggatc
    25801 aaatcgattt ttttgacttg tcctcgcttc aacggctttt cagcatccgc taacccatcg
    25861 atgttcatag gttcttttgt gtaataaaga cttcgttcct ctgctttttc tgtcagtctt
    25921 tcagctgcat atttatattt acggtattct aaaagctgag tgaccaattc ttctcgagga
    25981 tcttcttccc ataattcttc atcgtctgtg acgaattctt ggacaggaag caatgtcttg
    26041 cttttgattg ccatcaatgt agcagccatc actaagtatt ctcctgcaac agctagctcg
    26101 agtgttttca ttgtatggat ataattcata tattgctccg tgatcgtagc gatcggtata
    26161 tcatatatat cgatttccat ttgctgaatc agatgaagca gcagatcgag aggaccctcg
    26221 aatacatcta atttaatatt gatctccgtc aactcattca ctctttcttc tttttctttg
    26281 ctagccattt tccaatgtaa ggctctgtga tttcagttgc acggatggag acatccgggt
    26341 acttttcctg taattcatct aacatttgaa agcccatatt ctcaccgcga taagacggat
    26401 tcaaagaaat gtcatggatg atcagctgat cgttcgttgc ccagtaaata ccaataatcc
    26461 cttgaatgtt ggttgaacct tctggcgtaa agaaaaaaag ttcataatcg tcttcagatt
    26521 cgtacgtatc aatctccttc aacaaagaag ttttacctgt cagtttctta tggaaactca
    26581 atagtcccat agcaatttta cggttttctt tccggtaatg tgttaacatc ttctcacctt
    26641 cctatgacta agcccttgga aaatgctgct tataaacatc cgtcattctt ttcttcgtga
    26701 tatgtgtata aatctgagtc gtagagatat ccgcgtgacc taacaattcc tgtactgtcc
    26761 gcaaatcagc tccattttct agcagatgtg tggcaaagct atgccgcagc gtatgtggtg
    26821 ttacttcttt gtaaatccca gcctctcgta ctaactgctt cagatttttc cagattcctt
    26881 gccgagaaag acctttccca tgattattga caaatacatg catttcagat gcgtccttta
    26941 caagtaatgg acgtgcttcg tctaagtagc gttccagcca ttggatcgca taatcaccta
    27001 aaggaatgat ccgctcttta tctccttttc caactgtctg taccagtcca agagaaagat
    27061 gaagatctcc caatttcaat ccaatcaatt cactgacgcg catcccagta gcatacatga
    27121 cttccaagat tgcacgatct cttatcccta acgtttttgt cgtatcaggt gcttcaatca
    27181 gcttttctac ttctgtcaga gtcaatgtac ttggtaattt ctgtgctttc ttcggcgtat
    27241 cgatatgttg catcggatca tgatcagtga tacgctcttg ccgtaaaaac tgatggaatc
    27301 ttctgagact tgagatcatc cgagtaacag ttgctgtcgc ttttttttct tcatgcaact
    27361 tctccagaaa acggatgact aaaaagcgat cgacatcctg ccatgaagca attttctgct
    27421 cctgtagaaa atgcaagtac tgttccaaat cacgttcata gctttgtcgt gtattggcgg
    27481 acaatccccg ttcaatattc aagtagtgga gataatcgtt gatttgctct ttcataataa
    27541 tccccctcat gcctctctat catatcagat accgcttcat tctgatagtc tttttccata
    27601 atctgagcaa agaaaaagcc gtctagacac tcagacggac tggtttcttt tttctttttc
    27661 atagcattcc tgacagattc catggaacgt tagccgatga tcttttacta aaaaatgata
    27721 acgttcttct actacttgct ccacttcaag taaaagatcc tcttctactt cctcgatcgt
    27781 gccgcagttc aagcatagta aatgatgatg gaaatgtttg gcgccttctt ttctcaaatc
    27841 ataacgggct aatccatcat taaaacttac tttatcgaca attttcaatt ctgtcaagat
    27901 ttcaagggtt cgataaacag tagcaagtcc aatttccgga ctcttttttt tcactaaaaa
    27961 gaagatttct tctgcagata aatgctcttt ctcattttcc aaaagcacaa gcagagtagc
    28021 ttctctttgc ggtgtcagct taaaccccgc ttcatgtagc tgctgttttg tttttttcat
    28081 cgccgaaata gcattcatga ccatttactc aaccccaaca atttataata attacaaata
    28141 aaaaaagata taacactatg ttaattaaaa ttattataag ctagttaaga aaaacatgca
    28201 agggattctc aattagattt tttgactagt tttgtcacac cgttttcttg tgtaatcaat
    28261 ccttgtttca aaagattacc taatgcgcgc ttaaatgctc ctttgctgat accaaaagct
    28321 ttcatgattt cttctggatc cgatttatct gtaaagttga tttgctgatc tgcagaacgt
    28381 tctaaaatgg ttaaaatcat ttgtgcatca tctgatatag cttcatatgc tcttggcttc
    28441 aatgaaagat tcagtacacc gtctggtcgg acaccaatca ctcgaccttt tatctgctcg
    28501 cctagacgag gttcattata tcgttcagaa ggatggataa acccaagata gaaatcttcc
    28561 gttaaaacgt acgtacctgt taatttcaag cggtatgctg ttcctatcac atctttgttt
    28621 ttcatttctt ctgttccata tttgcttaaa gatttgaata ttttttcatc tgctaaactg
    28681 ccccaaatac ggtcttttgc atcaactcta agggaaatca tcaagcgatc ccctttttta
    28741 ggccatagct ctgtcattgt cggcaattca tcaagagata cgacaaagtc tttatctgga
    28801 agaccgatat cgacaaacgc acctaaatct tttcgtgtag cagtaacgat tccaaaagcg
    28861 taatgaccga tacggctttt aggaatttgt gtagtgatcc gattttcatg tttttgattt
    28921 tggtaaccga atccttcaac tgcttcaccg attttgtgtt caccttctga tttgcttaag
    28981 ccaaaggttt gtccgttttt ttggacaaag tagttttttt cattttcatc aataataata
    29041 cctgtaaata tagttgctaa taaatcgttc atttttttct ccctctatag tgaaaggttt
    29101 tcttaataca tttctttgct atcataacat aatgttatct acttgaatag ctgagccttg
    29161 gtttggaaag gtgaaaaaag gagtatgaca acagttgtgt catactcccc tttttcgaat
    29221 aaacggtgtt aaacggcagg ctcacagcct ctttcatttg cctaaaatta attgctccca
    29281 tttcctccga accatccagt tttacagttg atggttacga tgtaaaatgg caaagcactt
    29341 tctccaatgc ctttcatcaa tgcagatttt gttgaagtgg catatagatt ataatagcct
    29401 tccccatttt cgattctggc tttttctaat ctgtaacctc cttcaacgat atatccacgt
    29461 tggatacatg tagcaatcac tttgctttgg acacctggtg cccacgtcca tgctggatta
    29521 gatgcatcgg caatatcact tgctctggca gatttgattg gctgattcgc tgagtttgaa
    29581 gaggaagggg tcgaggatgc cgcttcttgc tgtgctgctt gtgcgaccgc agcttgttga
    29641 gcggcttgag ctgcagctgc tgcttgagcg gcttgttttg cttgttcttc agctgctttt
    29701 ttcgcttctt ctgctttttt cttttcctct tcttcttttt tctctttatc agtgagcatc
    29761 ttatctactt gtttcaattg ttcattcaac tgagcagata gatctgtatt tttcactttt
    29821 tctactgctt cttttgccgt cttgtattgg tcgcgtgttg ctgaatccac tacttttccg
    29881 tctttaaaga ctatagctgt tttttcttta gctgtttgca attcagcata ttgattttta
    29941 gcttctgttt gtgcttgttg aatcaatttt ccaaaatctg tgttatccgt ttcgattgct
    30001 tggatctctt tatctgcttt caacttgatg tcatctgcta gatgatcatc gacgatggca
    30061 ggtgctatga accactcatt cactttttgg atatctcgga tcttagattg gatatcttgg
    30121 taagttgttt ctaactctga atatcctttt tcattcttaa gactggataa atcatttttt
    30181 agttttgata aatcttgatg aatcatatct gcacgaataa attgatggtg atcatctgta
    30241 taaaaggcag caagtttctc atgaagttct tccatttgtt gctgtctttt tgcttctgct
    30301 gctacttcgg cttctacttt ttgatgatgg tcataataag cccagcttcc tccaccgagt
    30361 agaagtactg ctgctaaaac gagataaggt actttttttg attttttctt ttgcgcttta
    30421 tctggatctg ttttttctaa gtttgattct tgatgtgttt tttcagtatc tggcacgatt
    30481 gttattgatt tcgcttctgc ttcaaacagc ttaggctggt tctcttcttt ttctattttt
    30541 gcttcaagat cagtaccctc tttgcttaca gattgatttt cagcatcgga ttcactattt
    30601 atttcatcgg gctctgttga ctttttgtct gtgttttcga gtatttcttt tttctctgct
    30661 tccggcagtt tattcgtttt ttccttttct aattgttttt cagtaaatac ttcagttttt
    30721 tctgtgatat ccgtttttac gtcttttatt tttactgttt ctttacctgt ggcgttttct
    30781 ctatcagagt ctttttcagt ttcttgttcc ttttctggct ctactttgtt ttcctgtgca
    30841 attttttcac tgatcatttt gagcagctct ttcgcgtgct cttcttttgt atcttcacga
    30901 tgtgctttga tataagcgga tagtatcgaa ttttcttcct cattatcgac gatttctttc
    30961 agttcagcta ctcttctttt ttgttttgat tcaagcgttt cttcttgatt ctttcctgat
    31021 tcttccgaag tctcatgaac agttgctttc ttctgttcat ttttctctat attagttact
    31081 tgatcgtgag caggtggttc ttcgtcttct ggttctgctt tttcgaataa ttccattaca
    31141 gattctagag gtaaatcttt caattccgac cattcgattg gatcgtttaa ttctgtatca
    31201 ggaaagactg catcttcagt tgatttatcc tgcatagctg catctgtatt tgtttcttca
    31261 attgattctt ttatttgctg atcttctgca gttatttgat gaccgcagtc tggacagata
    31321 gtattctgct ccattttttt cgatccgcaa tttggacatt ttttcatcac ttagcgatcg
    31381 gctcctttac taggtttacg ctaccattta atctatcata ttctatacgt cacgccaata
    31441 gcttattttt aggatttact taaaaataat gaatgaattt ccttcctata gaaaaaaaag
    31501 cttgagactg cgtagtctca agcttttttt cttttaaaat aaaccaaaca gttttactga
    31561 tttctcttct ggtcaatagt ccacgcttat tctaagaaat cttttaattg ttttgaacga
    31621 gaaggatggc gcaattttct taatgcttta gcctcgatct gacgaatacg ttcccgagta
    31681 acaccaaata ctttccctac ttcttccaat gttctcgtcc ggccatcatc taaaccaaaa
    31741 cgtaagcgaa ggacattctc ttcccgatca gtcaatgtat ctagtacatc ttccaattgt
    31801 tcttttagta actcataagc tgcgtgttct gctggacttg ttgcttcctg gtcttctatg
    31861 aaatcaccta agtgagagtc atcttcttca ccgattggcg tttcaaggga aacaggttct
    31921 tgcgcgattt tcaagatttc tcggactttt tctgttggaa gatccatttc cgcaccgatt
    31981 tcttctggcg taggttctct tccaaggtct tgcagcaatt gacgttggat acgtatcaat
    32041 ttattaattg tttcaaccat atgaactgga atacggatcg tacgtgcttg gtcagcgatt
    32101 gctcgagtga tggcttgacg aatccaccat gtagcatagg ttgaaaactt aaatcctttg
    32161 cggtagtcaa atttttcaac tgccttcatc agtcccatgt ttccttcttg gatcaagtca
    32221 aggaactgca tgcctcgtcc tacataacgt ttagcgatac ttactactaa acgaaggttt
    32281 gcttctgcca gacgctgttt cgcttcttgg tcgccttctt cgattttcaa tgctaacgct
    32341 acttcttctt ccgcagttaa aagggagaca cggccaattt ctttcagata catacgaacc
    32401 gggtcattga tttttacacc ggttggcgca gaaagatctt ctgtttttgc ttgttcagct
    32461 tttttctcag cactcttcaa gctgtgaatt gatggatcac cattttcatc caccacactg
    32521 atacctgcgt cttcaatctt ttggatcaat ttgtccattt catctgcatt caaagtgaat
    32581 ggtgtagcaa gctgattgga taaatcatca tatacaactt gtcgtttggg tttgttttct
    32641 ttgatgaaag ctgctactgc tgcttcatac gtttttttat ctgttccatt tgccataaaa
    32701 gaaggccccc cttgagtagc tatttggctt gttttaactg tttcgttaaa ttgatgattt
    32761 caatcgtcaa ttcaagctca agctgtttgt ttccagttcg tctggcttct tgctgcattt
    32821 gtttttttaa tgttatttca tcagctaagc cagatttgct gatcacatct agcaagtctt
    32881 ggatttctcg tggcgaactt tcttctgcca ccggtaaata ttcgatttcc accaccaacc
    32941 gccttaaatt ttcatcttgc aaaaagtcta aaaactctgc aaggttgaat tcataatgtt
    33001 gtgagacata tgcatcgaat aaaaaataaa cttctgcata tgcatcatga ataaactgaa
    33061 attctcgttc ttttaaaata ttacggacag aaacttcttt aaatgcacga tataatagaa
    33121 tctgctctgc cttttctact tgcgtcaaag gtctcttttt ccgtgcagtt tcttgtctcg
    33181 taacctgagt gtgttgactt tcctgaggtt cttgacgatt ttgctgattt gtccttcgaa
    33241 gatcgcgcag ttgtttttgt atcgtctctc tgccgatctg aaactctgtt gacagttgag
    33301 atagatacat atcttgttcg attggtgaaa caactcggct aagttcaact agtaattctt
    33361 cgaggtattc gatcttgtct ttttcattat ctaaattttt cccttgtttt aaataacgtg
    33421 atttaaaagt aaagactgtt tcgcgcccat gagccactaa ttcttggaaa gatgcttctc
    33481 catacttacg gagatattca tcaggatcaa gcttttctgg aacgactacg acagaaagtg
    33541 ctaatcgact gttttgccga agcaattcaa tccctcgatt ggttgcttca aaacctgcgt
    33601 tgtctccgtc atagcaaaag accaattctt tcgtcagctt ttccatttgt tggatctgtt
    33661 gagctgttag acttgtcccc atagaagcta atccagtttt gataccagcc tgccatgcag
    33721 caatcacatc cataaaccct tcgaacaaca aaatttctcc gtttttccgg atatcgcttc
    33781 ttgctttatc aagattaaat aaaacttgtc ttttattgaa cagttctgtt tctggactat
    33841 tcaagtattt gggctgatct ttttcctgcc ctttttcagg tgcaagccag cgtcctgaaa
    33901 aaccgatcgt ctggccttga gaattacgaa gtggaaacat cactctttga taaaaacgat
    33961 cagacatacg gccatcatca tgcgtgacaa acaatccaga gcgttctaac aattcatttg
    34021 aaattgattc attcttgaat acttgctgaa gaaaagaacg ctcattaggt gcaaaaccta
    34081 tgttgaattc ttcaattaat tctaaagaca acccacgact ttgcaaatat tctaaagctg
    34141 gctgtccagc ttttgtatgc aatagcatgt gatgatagat ttccgctgct ttttcatgag
    34201 cagcaatcag tttccccgta gcagattcag gatttcggac agctatagta cgctgacgcc
    34261 attgatcttc taacggtatc tgttcgaagt ctgccacttt taccactgct tctggaaaac
    34321 ttagaccttc tagttcttga agaaaagtaa acacgtttcc gcctttacca caaccaaagc
    34381 aatgaaaaat ctgcttgtct tctgcaacag aaaaagacgg tgtacgttct tcatgaaaag
    34441 gacacagccc caagtaattt ttaccagact ttttcaactg gacatattgt ccaatcacct
    34501 caacgatatt cgtttgactg cgtactgttt cgataacctc ttgaggaatc cgttgagcca
    34561 ttttttcacc ccctgctcta ttgatatctt ttgggtttaa cagaagatat ttcaggagaa
    34621 taaccggtaa aaaatcgcac taactgaaat cagtgcgatt ttaaaggaca caattataca
    34681 tgtttgattg tatcatattt cattgctttt tcaagtataa cacatttcta tctcattaag
    34741 catcaatttg ctactctttt cagtaactga ttttgaatat cagccttcca gtacaatact
    34801 cgaagaacaa tatttacgag catgaataca aagaagtatc ctacgctctc tcccaaccct
    34861 aagtttactt ccaaaattcc atggaacagc attgcagcta catatatccc tacgatcgtc
    34921 gttactgcta ggaagatgcg caatgggttg aagggataac aagcttttac aactgccatg
    34981 caactgatcc caatcagcaa gtaatacatc aaagtagtag tttcagcatt tccccatgcg
    35041 aatgtttttc caagtagata caccgcaatg atattcacta ctacaagcaa ggcatttggt
    35101 aaagcattaa taagtgctgt aggcaaataa cggtaagtga tttttctttt atcttgttca
    35161 aaagataaaa agaaggaagg atacccttca atcgctaaat caatcagcgt gatttggata
    35221 gggataaacg gaaatccaat tgctgtaacg gcacatataa tcgataaaat aaatgaataa
    35281 atcgttttga taaaaaagat gcctgagact ttcgtcacat tgttgaccac tcggcgtcct
    35341 tcaaacagga cttctggcaa tgtagtaaaa tcagagtcca acaataccag attcgcaatc
    35401 tgacgtgttg ctccatcacc ttctgccata gcaatgctgc aatctgcttc tcttaacgcc
    35461 aaaacatcat tcacaccgtc gcctgtcatt gcaaccgttc tgccgctttc tttcaattca
    35521 tttaccagca atttcttttg ctgcggactc actcgtccaa aaaccgtata gcgatgagca
    35581 gcttctcgca cttccatttc ttcagtaatt tgagaaagat cgacatacgc atcataattc
    35641 ggtaatcctg cacgtctagc gatattggag acagtaaaag gattgtctcc agaaatcact
    35701 ttcagatcca caccttcctc atgaagataa gctagtgttt cttgagcgtt ttttcggatt
    35761 ggatcatcga tttcaaacag agcaaccggc tcaacaaatg ctggtttttc ttcgttcaat
    35821 tgctgatttg gagcgatccc caacataagt acgcgatagc cattcgcttg ggctgttttt
    35881 agactgtccg gaagccgatc ttcttccacc aaacgttcag gcgctcctac gtacacgact
    35941 cccagttctg gaaattccat cgcgccccat ttgcgatcag aagaaaatgc gactttgttt
    36001 gtcacatcat accgattaga aagctcaaaa taatctctca tagcccgcat agtgatgttg
    36061 ttatcactgc tttctgccag atacgtcccc ataatttctg ggatggcttt ctcgtacagc
    36121 ggatgtagtg cttctacccg ctggatactc attttccctt cagtaatggt ccctgtttta
    36181 tccaagcata atgtatcgac atgggccagc gtttcaattg aatacatgtc ttgaacaagg
    36241 attcgttttt ttgccagttt ggttacccct gtcgttaatg caatacttat tagcaatacc
    36301 aaaccctttg gaagcatgcc taacaaagct gctgcagatg caataacaga aattttgata
    36361 gaagcatcgc ggatgaacag tgcttccaaa aatagtatga tccccaaagg aataatcacg
    36421 aaactagtga atttggatac tttgcgaatc gaatctacaa gttcagaacg aatagctttg
    36481 tgagtctttg cctcgctagt caagcggaca gcataattat ctgcacctac tcggataact
    36541 tcagccatga cagaaccgct tgtcaaaaag cttccagaaa gcaattcagc tccagtttct
    36601 ttgacaatca aatcagattc gcctgtcaaa agagcttcat ttgcttctgc ccgtccagat
    36661 ataacctgta aatcggctgg tatctgttct cctgctccga tttttaccaa atctcctaat
    36721 accagctctt tagtagaaat cgattgttct ttaccgtccc ggatcactag tacttctgat
    36781 tgggaaacaa tcgataattt ccgtacaaga tttcttgcat gtatttcttg ataaattcca
    36841 ataacgatat tgacgacaat aattgctaaa tacgccatgt tggaatacgc acctacaaat
    36901 aacagacaaa tcccaatacc aaagttcaga aaattgaata acgtcaatat attatcgctg
    36961 aatatttgtt ttgtactttt tgatgcatct tcctggtagt cattttgctg gccagaagcc
    37021 attttctgaa ctacctcttg ctgtgtaagt ccattcattt tctttttctc cattttccgc
    37081 caactttcaa aaataatcac gtgtttaagt ctagcgtgtt ccaagattaa aaacaacttg
    37141 tttttataaa atttatagaa aaacacacga atgattttac aaaaaagatg ccaacctttt
    37201 tgtttgaatc acccgtgtgc tgtttttatt atcaatctat ctttacttat attgttcctc
    37261 caaacggctc atccaccaac ctagtgctgt accggctata aaaagaacag gacaagctag
    37321 taccccagca actcccaatc ccgcgtaatc ggtaggaatg atcataatcg ttgaagcaaa
    37381 aactacacct aaaatgaaat ggaatagctt gctatatgcc tttgagaaaa tgtaatccat
    37441 gatttttgac aatccaagaa cacaaagaat cccaccaatt ccgatcggca caatcacttc
    37501 aagtcgtact tctttaaatc cgtcagccat cgctttatac aatcccatat acaccaaaaa
    37561 attagaaggg ctcaatcctg ggacgatcat acccaaacca atcaatccgc cggcaatcag
    37621 ccatgttcca aaattctgat cgacttgccc gtcaaataat tgagcgccgt ataataaaaa
    37681 caaataagcc agaacaaaag taacaaccat gatgatcaca tcagaagtat ctcttccttt
    37741 tttccctgct tctttccata acgcaggaac agttccagcg atacatccga caaaaaacca
    37801 taatatcgtt gtttcatacg cacctaataa aaaactgact aaaaaagaaa gtagaaagat
    37861 gcctgtcact ccgcctagac caacaggcag aaaaaacaaa acattttctt tgaaattttt
    37921 tgtaaggtgg gctaaaaaag aaatcagccg ctcatacatg ccaaagacag ctgctaatgc
    37981 accaccgctg actcctggca agataaatcc tgaaccgata aacattccct tcacaaaccg
    38041 taacatccag tctttggttg tgtaccccac ttcttgattt tccatttttt gccttttttc
    38101 cactatttgc gtactccttc tttcatttaa ttttattcat gattgaacat ttcctgctat
    38161 tttacttaaa gactttttca taggtctcta ttattttttc attcattttg acaatatcag
    38221 gataaaacat gttacctcca ttatgataga gatagccgcc attgtaaagc aaggactgga
    38281 tccgccaata tcggtattgt ttcccttcct catttcctaa aagtgggctc agcacatctt
    38341 tagaatacgc ttctgctaat tctactgtgt tttctccgcc atgatttttt acataggcaa
    38401 tataatcttt cccaaaatta tatgcttgga cagctgtcca taaatcgcat ccttgtttct
    38461 ttgcttctac tatcatttcc gccaaatatt tgacaccttg atcaatactg tcagctggat
    38521 tatcaatgat ctcagcttct ccatgcagac tttccgagct ctgcatgaga tctgtccctt
    38581 ttccttttga ttcggtcaat attatcgcct tgatcaaatt ctcgtagttt tccatatgat
    38641 aattttttgc agctgttgtt atttctattt gatagctttc cactcgtttt accgaacgat
    38701 aagttcttcc tcataaaaac aacaccgcac caataaccgt cagaatgatc atgtaaaaaa
    38761 gaatacggat aatagataat cgttttcgct tcatttcatc acctagttat tttgacatct
    38821 ttttttatct tactatttct ttatcttctt ggctatttct tccttgattt ctgtgtacgc
    38881 cttaagaaaa aatgaatttt cctgtatttt ttgacatctt acgaaaatga tcgttgtttt
    38941 tcagtaaaaa caattgccca atgactgata taatcgttat actttacaca gtaggtgatt
    39001 atgtgaaaaa agaaaagaaa tatgcaaaaa tgccagatgg gagcgaaatt tactatgaaa
    39061 agagcggtca aggcttccct ctctttttac tgcatggcaa tgacggcagc ggccgctttt
    39121 tttcagaaca ggtccctgta ctcgaacgct actataccgt gtatctagta gatagccgag
    39181 gacatggtcg ttcgacaaac gaagcatcta tgttgaattt tcagttaatg gccgaggatc
    39241 tgaatacgat catgttgctg gaaaaaatcg atcaggcaga ttttcttgga tttagtgatg
    39301 gagcaaatct agcactggta tttgccagca gcttccctaa aaaagttcat cgattgattt
    39361 taaattctgg aaacacgctt gttaaaggcg ttcgtttttc tgccagagtc atcagtaata
    39421 ttcattatgc atgggtatgg ttactctctt tatttcgccc aagtcttcgc aaaaatcttt
    39481 tagttatcaa acttttgctg catgacatcg ggcttacaga aaatgattta aaaaaaatca
    39541 actcccccac tttgatcatc gtgggaaaaa aagatgtaat caagctaaaa cattctttgt
    39601 atatagctaa aactatacca aaagcttcct ttgtgcttgt gaaagaacaa ggacatgaat
    39661 tagctagaaa agatcctgaa cgcttcaatc gagaagttct tcaatttctt tctgaaacat
    39721 aactattagg aatagaaggt gatttatttg ttaaaaaata ccatacaatg gctgaaaaac
    39781 cacctaggac tgttcaaaac aatctttttg atttctgtca ttgtcattat tgtcggtgaa
    39841 ttgatgtcca tcggtaaaac tctatctatc cagcaattag gagaaacgtt tgccactata
    39901 cctgtatgga aaagcggact aatgctattg attggtctta tctctgtgtt acctatggtt
    39961 ggttatgata ttattttgaa tcggatgctt gaacaaaagc agaatcctcg ttacctgttt
    40021 gagacaagct ggctgatcaa tacgatcaac aatattgccg gtttcggcgg gtttgttagt
    40081 gtggggcttc gctctgaact atatggacaa aagaaagaga gcagaaacgt gatccaagca
    40141 ttatcgaaag ttttcttgtt tttgatggct ggactttcta tttacagtct gctttctttt
    40201 ttacttgtag tcttcacgcc agttgctcct tttttgaagc aatattggat atggttgatc
    40261 ggcggcggtc tatattttcc tgtcgtcttt ttatttacta ctttcaaaaa gaaaggattc
    40321 cttggtggta cctcacttaa aactcaagga cagctattgc tagtttcatt gttggaatgg
    40381 accggtgtcc tgtttagttt cctttctgtt ggttatttaa tggaaatccg gattgatctg
    40441 tggcaaacaa ttccattatt cattgctgct tcagtgatag gaatcgtgtc tatgattcca
    40501 ggagaaattg ggagtttcga tgtaatgatg attatcgggt tgtccgctat tggtgtgcca
    40561 agagaaatag tcgttgtgtg gatcttgctc tatcgcctgt tctactacat cattccgttt
    40621 ttgattggaa tcgtcttttt cttcaaaaat atcggttcca cttttgatca gcgttattct
    40681 ggtatcccta aacaattagc aacagaaatt gcgcataaaa tcgtggttgt cttattatac
    40741 ttttctggaa tcatgctagt actttctgcc actatcccgc aagcttttac agagtttcgc
    40801 tggcttcata gtttgaaccc attgaaattt cattttatta tccaatttcc tagtatacta
    40861 ttgggatttc ttttaatcat tatgggcaga gggatcgcag cacgtgtgaa acgagcatat
    40921 ttgcctacaa tcttcttgat tgttctagct ttactttatg ttttattaag tgattttagt
    40981 ttcacaccgg tggttttctt gtctattctg cttctgatca ttcttgcttc taaaaacgaa
    41041 ttatttcggg aacaattgat ctattcatgg gagtggcgta cgatcgatgg aatcattatt
    41101 ggtgcactgt ccttgcttta catcgttatt ggggtataca atctgccgga tttccctcat
    41161 cggcgtcacc attttatttc ctttttctta tttccttctg aaaagatttg gttttcggga
    41221 ttgttggcca tcatcgctgt tagtttcatg atcgttctat ttgttcattt ccttcaagga
    41281 gaaaagaaac agattggcga agcctttaat gaagaaaagg ccctgaagat ccttacaact
    41341 tatggaggaa actccgatag tcaattgatc ttcttaaaag ataagcggat gtttgcctat
    41401 gaaaaagacg gtgaaccaac tgttcttctc caattcgctt gcttcaataa taaatgtatt
    41461 gtaatgggtg atccttctgg taagaaagaa gattttccag aagcaatcga agcttttatc
    41521 gaagaaacag atcgactatg ctatttacca gttttctacg aaacaagcga agagattgtt
    41581 atgattttgc atgaattcgg ctacgatttc attaagatgg gggaagaagc ttatgtagac
    41641 ctgaatagtt ttacaacttc agggaaaaaa atgaagggca ctcgtgctgt cttgaatcgg
    41701 atcgaacgag aaggatttac atttgatgtc ttacaaccac cattttctac tgagcaaatg
    41761 agtactttca aaaatatctc tgataattgg ttgggttcac gaaaggaaaa agggttttct
    41821 ttaggctttt tctctgaaga ttatctacaa agagcgccta ttgcagttgt caaaaatacc
    41881 caagaagaaa ttgtcgcttt tgcaacgatc atgcctactt acacgaacaa taaagtagga
    41941 acgattgatc tgatgcgata tgattctaaa aaagcacctt ctggcagtat ggatttcttg
    42001 ttcttgcatt tgtttgccta tatgaaagaa gaacatatcc aatggttcaa tttagggatg
    42061 gcccctcttt caaatgtcgg aacttccaga aagagttttt tacaggaaag gattgcatac
    42121 ttggtttacg agtttggttc tcatttttat tctttccatg gattaagaga atataaaagc
    42181 aagtacgcta caaactgggt ctcacgttat actctatatt ctagagatag ttggattgca
    42241 tatgttatga ttgctttgct gattattgat aatgcgccag ttgagcagac acaaggtaga
    42301 ataagtggtt tgaaaaaatg gatacgaaga aagtattaaa aactgcatac cgatagatga
    42361 acactctcat gatgaataaa agagactgaa gtaaaacgaa agttcatgct tctacaatct
    42421 cttcttatac aattgaagaa caaagagcgg tccaaacaga aatcctgttt ggaccgctct
    42481 tttattttaa acatttttca ttaccaaaat attcgagtcg cgtttcttat gcttcgatct
    42541 ctgtaacgat acctgaacct actgtccgtc cgccttcacg aattgagaat gtcgttccgt
    42601 tttcaacagc gattggatgg atcaagtcaa catcaatcgt cacgttgtca ccaggcatga
    42661 ccatttctgt atcttctggc aatgtgattg ttcctgtaac atcagttgtg cggaagtaga
    42721 actgtggacg atagttgttg aagaatggag tatgacgtcc accttcttct tttgtcaaca
    42781 cgtacacttc tgctttgaat tttgtatgtg gagtaatgga acctggttta gcaagtactt
    42841 gtccacgttc gatatcgtca cgttggatcc cccgtaacaa cacgcctacg ttatccccag
    42901 cttccccgta atctaacgtt ttacggaaca tttctacacc tgttacaact gctttttgtg
    42961 tttcaggttt gatccctacg atctctacct catcaccgac acgaacagca ccacgatcaa
    43021 tacgacctga ggcaacagtt cctcgacctg taattgagaa gacgtcttcc actggcaata
    43081 gtaatggttt atctgtatca cgttctggtg ttgggatata ttcatctact gtatccatca
    43141 attccataat agcagcttca gcatctggat cgccttgcaa tgcttttaat gcggaacctt
    43201 tgatcacagg agtgtcatcg cctgggaaac catattcgct caataactcg cgaacttcca
    43261 tttctaccaa atcgatcaat tcttcatcat cgactaaatc aactttgttc aagaaaacaa
    43321 tcaggtattt tacaccaact tggcgtgaca ataaaatgtg ttcacgtgtt tgaggcatcg
    43381 gaccgtcagt tgcagaaaca actaagattg caccatccat ttgtgctgcc cctgtaatca
    43441 tgtttttcac atagtccgcg tgtcctggtg catcaatatg cgcgtagtgg cgtttttctg
    43501 tttcatattc tacgtgtgcg gtattgatcg taatcccacg ttcacgttct tctggagctg
    43561 catcaatact agcatagtct tgaggatttg ctaggccttt tttgcctaat actgtagtga
    43621 tcgcagcagt taaagtagtt ttcccatggt caacgtgtcc aatcgtacca atgttaacgt
    43681 gtggtttact tctgtcataa tgttcttttg ccataattta caaacctcct aaataatata
    43741 gatcactgaa aaatttcagc tctcaccttt ttattatagt ctttttttga ctaaatcaaa
    43801 aagaatacgt ccgacttact ttttgtaaga ttcgaactaa gcttattata cgtcaatatt
    43861 ggtcaaagtc aactaagagc actcgtctgt cttatacgcg aaaaaagtac cttttataaa
    43921 taaaaaaatg tgttttttga gaaaaacttc aagatagtcc ttaactttct tttgaagcaa
    43981 taagaacaaa atgaggaaaa gtttaaaatt cagctgttgc caacaacttt caatagacga
    44041 aatcttctat ttcgttaata ataaggaaag aggtgaagta catgaaaaaa ttaattggcg
    44101 tacttgtcgt tttaggcata tttctcggcg gaagttttgc agcttatcat tatttttacg
    44161 gcggcgaagc ttattataca aaaatcacga cgaatggcga aatgtcagct acccaagcag
    44221 ataacggaga gaaattcacg acttatacct acaagcaagc ggcttataat aaagatggtg
    44281 aaaaaaaaca ggtgacgctt cgcgaagaaa gagaacatcc attaaagatg aatgcgtatc
    44341 ttaaactcaa agtcaatcct cgtaaaggtg tattaagctg ggaagaaata aaggcttctg
    44401 aagtgcctaa aaatgcggca gaaaaaatcg ataattagta aatggtctta aaattttatt
    44461 agataaaa
//
LOCUS       DAZKLA010000009        43903 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000009 DAZKLA010000000
VERSION     DAZKLA010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.009"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(108..452)
                     /locus_tag="ACG85N_000388"
     CDS             complement(108..452)
                     /locus_tag="ACG85N_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0223188.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(445..879)
                     /locus_tag="ACG85N_000389"
     CDS             complement(445..879)
                     /locus_tag="ACG85N_000389"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFQ0223189.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            1017..1754
                     /locus_tag="ACG85N_000390"
     CDS             1017..1754
                     /locus_tag="ACG85N_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0223190.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            1747..2958
                     /locus_tag="ACG85N_000391"
     CDS             1747..2958
                     /locus_tag="ACG85N_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0223191.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            3162..3944
                     /locus_tag="ACG85N_000392"
     CDS             3162..3944
                     /locus_tag="ACG85N_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFQ0223192.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            4014..4676
                     /gene="trmB"
                     /locus_tag="ACG85N_000393"
     CDS             4014..4676
                     /gene="trmB"
                     /locus_tag="ACG85N_000393"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFQ0223193.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(4774..6528)
                     /gene="ade"
                     /locus_tag="ACG85N_000394"
     CDS             complement(4774..6528)
                     /gene="ade"
                     /locus_tag="ACG85N_000394"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFQ0223194.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            6660..7589
                     /gene="comGA"
                     /locus_tag="ACG85N_000395"
     CDS             6660..7589
                     /gene="comGA"
                     /locus_tag="ACG85N_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFQ0223195.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            7546..8595
                     /gene="comGB"
                     /locus_tag="ACG85N_000396"
     CDS             7546..8595
                     /gene="comGB"
                     /locus_tag="ACG85N_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFQ0223196.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            8592..8870
                     /gene="comGC"
                     /locus_tag="ACG85N_000397"
     CDS             8592..8870
                     /gene="comGC"
                     /locus_tag="ACG85N_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFQ0223197.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            8845..9315
                     /gene="comGD"
                     /locus_tag="ACG85N_000398"
     CDS             8845..9315
                     /gene="comGD"
                     /locus_tag="ACG85N_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFQ0223198.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            9287..9634
                     /locus_tag="ACG85N_000399"
     CDS             9287..9634
                     /locus_tag="ACG85N_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223199.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            9597..10022
                     /gene="comGF"
                     /locus_tag="ACG85N_000400"
     CDS             9597..10022
                     /gene="comGF"
                     /locus_tag="ACG85N_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFQ0223200.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            9994..10353
                     /gene="comGG"
                     /locus_tag="ACG85N_000401"
     CDS             9994..10353
                     /gene="comGG"
                     /locus_tag="ACG85N_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFQ0223201.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            10525..11532
                     /locus_tag="ACG85N_000402"
     CDS             10525..11532
                     /locus_tag="ACG85N_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0223202.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            11557..12741
                     /locus_tag="ACG85N_000403"
     CDS             11557..12741
                     /locus_tag="ACG85N_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFQ0223203.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(13178..14761)
                     /gene="abc-f"
                     /locus_tag="ACG85N_000404"
     CDS             complement(13178..14761)
                     /gene="abc-f"
                     /locus_tag="ACG85N_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFQ0223204.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(14866..14994)
                     /locus_tag="ACG85N_000405"
     CDS             complement(14866..14994)
                     /locus_tag="ACG85N_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223205.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(15339..15482)
                     /locus_tag="ACG85N_000406"
     CDS             complement(15339..15482)
                     /locus_tag="ACG85N_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFQ0223206.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(15705..16184)
                     /locus_tag="ACG85N_000407"
     CDS             complement(15705..16184)
                     /locus_tag="ACG85N_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0223207.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(16257..16541)
                     /locus_tag="ACG85N_000408"
     CDS             complement(16257..16541)
                     /locus_tag="ACG85N_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223208.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(16619..16813)
                     /gene="ssrS"
                     /locus_tag="ACG85N_000409"
     ncRNA           complement(16619..16813)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG85N_000409"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(16885..18171)
                     /locus_tag="ACG85N_000410"
     CDS             complement(16885..18171)
                     /locus_tag="ACG85N_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFQ0223209.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            18309..18980
                     /locus_tag="ACG85N_000411"
     CDS             18309..18980
                     /locus_tag="ACG85N_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFQ0223210.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            18946..19434
                     /locus_tag="ACG85N_000412"
     CDS             18946..19434
                     /locus_tag="ACG85N_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFQ0223211.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            19447..20394
                     /gene="prmA"
                     /locus_tag="ACG85N_000413"
     CDS             19447..20394
                     /gene="prmA"
                     /locus_tag="ACG85N_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFQ0223212.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            20395..21159
                     /locus_tag="ACG85N_000414"
     CDS             20395..21159
                     /locus_tag="ACG85N_000414"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFQ0223213.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            21392..23605
                     /locus_tag="ACG85N_000415"
     CDS             21392..23605
                     /locus_tag="ACG85N_000415"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFQ0223214.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            23616..24062
                     /gene="dtd"
                     /locus_tag="ACG85N_000416"
     CDS             23616..24062
                     /gene="dtd"
                     /locus_tag="ACG85N_000416"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0223215.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            24233..25492
                     /locus_tag="ACG85N_000417"
     CDS             24233..25492
                     /locus_tag="ACG85N_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFQ0223216.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(25581..26528)
                     /locus_tag="ACG85N_000418"
     CDS             complement(25581..26528)
                     /locus_tag="ACG85N_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0223217.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            26922..28214
                     /gene="hisS"
                     /locus_tag="ACG85N_000419"
     CDS             26922..28214
                     /gene="hisS"
                     /locus_tag="ACG85N_000419"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFQ0223218.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            28229..29998
                     /gene="aspS"
                     /locus_tag="ACG85N_000420"
     CDS             28229..29998
                     /gene="aspS"
                     /locus_tag="ACG85N_000420"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFQ0223219.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            30107..30982
                     /locus_tag="ACG85N_000421"
     CDS             30107..30982
                     /locus_tag="ACG85N_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0223220.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            31248..33131
                     /locus_tag="ACG85N_000422"
     CDS             31248..33131
                     /locus_tag="ACG85N_000422"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0223221.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(33206..33490)
                     /locus_tag="ACG85N_000423"
     CDS             complement(33206..33490)
                     /locus_tag="ACG85N_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0223222.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(33480..34214)
                     /locus_tag="ACG85N_000424"
     CDS             complement(33480..34214)
                     /locus_tag="ACG85N_000424"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFQ0223223.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            34343..34966
                     /locus_tag="ACG85N_000425"
     CDS             34343..34966
                     /locus_tag="ACG85N_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFQ0223224.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            35158..36279
                     /locus_tag="ACG85N_000426"
     CDS             35158..36279
                     /locus_tag="ACG85N_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0223225.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            36286..39414
                     /locus_tag="ACG85N_000427"
     CDS             36286..39414
                     /locus_tag="ACG85N_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0223226.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            39433..42627
                     /locus_tag="ACG85N_000428"
     CDS             39433..42627
                     /locus_tag="ACG85N_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFQ0223227.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(42773..43480)
                     /locus_tag="ACG85N_000429"
     CDS             complement(42773..43480)
                     /locus_tag="ACG85N_000429"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0223228.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14175 a         7783 c         9251 g        12694 t
ORIGIN      
        1 cgttgtcgtg gtagtttcaa acaaaattca aaatttcatc acattcctag gaaatacgca
       61 aacttttctc acttatatag ttaaatctgt tgagaaaata atcattatta tatttgttca
      121 ggatactctg gcagatcagc ttttagtttt tctacttgtt cattgatttg tgtgatacgt
      181 ggttcggctt ggaatttaaa atcttcgata tcttttttga ttgcttcttg gaaagcagga
      241 atcacagatt cagctgtttc ttttgtttcg atcaatgcgc ttttaaaatg attcaggctg
      301 ttatttaaat catttgtcag agttgttgct tcttccaatt cttcgatcag tttctttctt
      361 gtttcattcc cgctcctggg cgccaataac agacctccga cagtgccagc agcagctcca
      421 aataacagtc ctttgaaaaa gtttttaatc atcgcaggac acctgcttta cgattgtttc
      481 ttgaatagct tttagttctt ccgatccata actatcttga tgattgacaa agtgaataga
      541 aaaatcatcc tctttgctgt agcgagggac taaatggata tgtgaatgaa aaacagattg
      601 ataggctagt tcacggttgt tattcaagat attaagacct ttcatttctg gaaaagcttt
      661 ttccaatgct tgagcaatct taggcacacg cgaaaaaacc tctcctgcaa gttcgggatc
      721 atattcaaaa atatctgcta catgacgttt aggcacaact aatgtgtgtc ctttcgttgc
      781 ttgagagatg tctaaaaaag catagacctt gtcgtcttca tatattttat agcttgggat
      841 ttcttgattg atgattttgc aaaaaataca gttttccata taaaacatcc tttctataaa
      901 gaataaatac ttcagcttaa ctttaccata agttaaagct attgtaagga aaaaaacaaa
      961 ataccgtcat tttcttcgtg gtataataaa taaaaataaa tggcggagga aaaagaatga
     1021 gtttaattat cgatcatgtc acaggggggt acggacatct ccctgtgtta aaagatatca
     1081 gtttcgatgt acagcctgga gagatagtag ggttgattgg actgaacgga gcagggaaaa
     1141 gtacgaccat caagcacatt atcggtctgc tggaagcttc aaaaggaacg atagaaatca
     1201 atcatctatc cttaaagaaa aatacggaag aatatcgcaa acaaattggg tatattccag
     1261 agactccttc tttatatgaa gaattgacat tgagagaaca tatcgaagtc actgcgatgg
     1321 cctacgatat acctatggat atagcaatga aaagagcaga aaagttgttg gagacttttc
     1381 ggttatctaa tcgtcttgac tggttcccag ctaatttttc aaagggaatg aaacagaaag
     1441 tgatgatctt atgcgccttt ttgatcgagc cttctttata tatcatcgac gaaccatttt
     1501 taggcttgga tccactagct atcaatgcat tgcttgaatt gatggtagaa atgaaaaaag
     1561 aaggagccgg tattttgatg tctacgcata ttttagctac tgcagaaaaa tattgtgatc
     1621 gttttgtggt cttacataac ggagagatac gagcagacgg ttctcttgag atgttgagaa
     1681 aagaatttca gctgccggat tcttctttgg acgaaatcta tattgcttta acaaaggagc
     1741 aggcacatga gtagtttttt tcaaaaacgt ttagcaagac atcaaaagaa aatgatgaaa
     1801 tatatgcgtt atgtattgaa cgatcatttt gttttagttt gtcttttctt gattggcggt
     1861 gtgggattat attattctaa ttggttgaaa accctgcagc caccgtttgc aattggaggg
     1921 ctcataatct ctgtattttg gatgctctgt ttattcgtgg gtaagatggc tacttttgca
     1981 gaggcagcag atattgtttt tttacttcct aaagaaaaag aaatgcaaac ctatttggaa
     2041 cgagcttttc gctattcttg catttttccg ttgatctttt tgatattggc atgcggattg
     2101 gccatgccat tagtggtggt tagtacggag caatcctttt cgatgttttt cgtctatctg
     2161 atcatattat ggagtttgaa attcagtcat ctgcaaatca agagagcaga attgtttcga
     2221 ctagatcaac aagttattcg ttattggaaa ttaggctggt ttttttcttc tctgatcttt
     2281 ttgtttctat cattttattg gctaatatgg gcaggaatag caggtgcatt agtacaggca
     2341 agtctatatt actggttttt gtggaagcaa caagaaaaag gattggattg ggagaaaatg
     2401 gtgcatgtag aagaacatag gttgcatcag atctatcaat ttatcaattt attcacagat
     2461 gttcctgaaa ttacggcaaa gatcaaaaga agacgctatt tggatcctat attgactcat
     2521 attaaaagag aatcaaaaaa cacttatctt tacttgtttg caagaagatt ggcaagaggt
     2581 tctgatttta gtggtcttta tttccgatta gtaattatag gtgggttatt gattgccgga
     2641 gtagtcgact tttattttag tctgattatc ggtttcttat ttttatattt gatcggtttc
     2701 caactgttac ctttagcgaa ccagttccgc tatatggttc tcgttcagct ttatccaatc
     2761 cataagaatc agaagaagca ggcgattcag tatctactca catgggtatt aattatcgcg
     2821 tcggttattt ttggtttgat tgctgcagtc gtattagatg ggcagcagcg atgggttccg
     2881 ctagcggctt atttgattgt tactatgctc tttgttaaac tatatgtacc aactcgtttg
     2941 aagaagatgt cagactgaaa acaaagctgt gtagggtaaa aatgagaatg aaaaatagag
     3001 cgttgacagt aagaatgctg ttcgttctat tttttttgag aaaagctggt tttttgaatg
     3061 gcaattaaat gaaaaagaac gtatatgtgc ttgacgtgta gtgttcaggc gactagaata
     3121 ataagtactg caaaacaaag agtaagagga cgggagaatg catgatggag tttcagttag
     3181 accaagagtg gcggttacgt cccatcaaag gcgacacagg aaaaacatac ataggcatgc
     3241 gtgatgagga caaagtcttt attaaacgaa acacgactcc gatgttggct gctctttcaa
     3301 gggaaggaat tacaccaaaa cttgtttgga caaaacgaac ggggaatggc gatactttga
     3361 cagcacaaga gtggctggaa ggtcgcttgc tgactcctaa tgagattggt caaagaaacg
     3421 acgtgattga tgttttgtac catttacatc attcaaattc tcttaaaacc atgctgaccc
     3481 gtatcggtgg tcgtgtcatg acgccggaga agatgctgca aaaatatgaa aaagaattac
     3541 cggaagaact ccagaaaaat caatttctgc aaattgtcca tcgttttctt cgtgagcaga
     3601 tcccgtcaat agataaggaa aacttatcag tagtacacgg agatgtaaac catcgtaatt
     3661 ggttagtatg taaaaattat ttatatctag tcgattggga ttcaatcatg tttgcagacc
     3721 ctgcggtgga tattgcaacg attttaggaa attatgtgcc actgtctagc tggaatcagt
     3781 ggctgatcag ttatggcata cgcccgactg acgaaatgct tgaaaaattt cattggtatg
     3841 cggccatgaa tttgctccaa gaaatcacca gatattgcaa acgtgaagat catcgacgga
     3901 tgaacgaaga aattttacaa ttaaaacgaa ttttcagtgg ctgaggtttg gcccaaactc
     3961 tgcggagatt tggccagacc tttttaatta gaggaaagga atggagataa ataatgcgtg
     4021 tccgaaatcg tccgggagca gcggaaatgt tagcggctca cccgaatttt gtaattagtg
     4081 atccaacatt gtggaaggga aaatggaatg aattgtttga aaacgatcat ccaatccaca
     4141 ttgagattgg tatggggaaa ggccagttca tcactggaat ggcaaaagcc catcctgaaa
     4201 tcaattatat tggagtagaa atgcaggtca gtgttgtttc gattgcacta gacaagttga
     4261 tcgaacaacc gcttcctaac ctgaaacttc tacatgtgga tggatcagcg ttgacagagt
     4321 atttcgctga cagcgaagta gatcaaatct atctgaattt ttccgatcca tggccaaaaa
     4381 aacgtcatga aaaacggcga ttgacatata agacttttct tgctgtagat gaacagattc
     4441 ttcgtccaaa tggagaaatc catttcaaaa cagataatca aggattgttt gagtattctc
     4501 tggcaagttt ttctcaatat ggcatgatcc tcaaacaggt atggttagat ttacatcaaa
     4561 gtcaatttga aggcaatatt atgacggagt atgaagaaaa attttcttca aaaggtcaac
     4621 gtatttatcg agtagaagct caatttcaag ataagaccaa aaagaaaagt ctgtgaaact
     4681 aactttctgc tttcataaaa acattaatat ccataaataa atcccagaaa atgaacatta
     4741 cagatgtaat gtattttctg ggatttattt gatttaaaat gtatttattt catcggtttt
     4801 tccacaaggt ggaatcaatt ggaagcgaga aaatgaaaat aatcctttgt cggtgatctt
     4861 caattcgggt atgacagata atgttaagaa agaaagtgtc aaaaaaggat caaaggtttt
     4921 agctgctcct agttgatgtg ctttagttat taattcattc aactgttcat agacgatcga
     4981 aaaaggttcc tgtgtcatga ttccgcctat aggaagagaa acacaagcta tttcctgctg
     5041 tttgttgaca acggtcatcc cgccaccttt ttgaatcaaa agattagcag catacagcat
     5101 atcctcgtcg tttgtcccaa cgacaactag attatgcgag tcatgggcaa tcgttgtcgc
     5161 tatcgctcct tgtttcagct gaaatccttt cactatgcct aatccaatgt ttcccgtttg
     5221 atggtgacgc tcgatcaccg caatctttaa ttggtctttt tcgattgaag gaatgaatcg
     5281 ttcgccttgt cggtccactt tttctaccaa atctttagtc actagactat tcgggataat
     5341 ttcgatcaca tgggcttgtg aagaagaaag aagcagctct aacgaatgtg gatttaatgg
     5401 ttgagcgttt agttttggca gacaacacgt atatgaattt gaatgatcag caaataaatg
     5461 gtcttggagt tgcccagatt gaacaactaa tgttccctta tagaaaactt catgaattgg
     5521 cagcttggta agatcgtctg ttaagaagaa atcagcttga tacccagggg caatcgcacc
     5581 tagtgttttc aaatcaaaac attctgctgc attcaatgtt gccatctgaa tggcttgtaa
     5641 tggatcgatt ccaagtgcaa tagcttgacg gacgatatga tcgattgatc cttcttttac
     5701 taaatcattg atcagtttat catcggtgac aaagaagcat cttcttgaat tttcaggtgt
     5761 gatcgccggt agtagcgatg ccagatcttt tgcaacggtc ccttctcgaa ccattaaata
     5821 cataccaaga tccaaacgtt ctttagcttc ttttgcagtt gtagcttcat gatccgtccg
     5881 aatacctgct gcaggataaa catttaactc ttccatgccg ataccagctg catgcccgtc
     5941 aatttttttc ttatgctggt gcatcaattg gatcttttct aggatgtctg tttcattatt
     6001 tgctactgct tgatagttca tgacttcagc taagcctaaa acttcgggtt tttgtaaaaa
     6061 aggggctaat ttttttgcat ttaaatttgc accattggtc tcaaaagggg ttacaggtac
     6121 acaagaaggt agcataaaaa agatattcat gagtgtttgt cttgcatctt ctatcataaa
     6181 ctcaagacct gtactcccag caatgtttcc tatttcatgg ggatctgtca ctacagtagt
     6241 aactccatga agcaaggata cttttgaaaa ttcgcttggt gataaaagtg agctttcgat
     6301 atgcacatgg ccatcgatga atcctggcac tagatattgc ccattagcat tatgtacgct
     6361 ttttccttcg tattcaccga ttcctacgat cattccttcg cagattgcta catctttttc
     6421 catgatttct ttcgtaaaga cgttgacgat cttagcatgc ttgatcacta ggtcagcagg
     6481 ctttttttta cttgctactt cgatatattt ccttaatgtt gtttccaatg gagtgcctcc
     6541 caagataaat ttttataagt ataacataaa ttcggttttt gccaatattt ttttgtaaat
     6601 tcattttatt tgaaacaaaa agatcaaaaa gacgtatgtt ttttgtggag gggagaataa
     6661 tggaaataaa acgattatcg gatcaaatga taagtgaaag ttttgccaaa catgcccaag
     6721 acgtatatat attgccagct tcagaagtat tttacatcta ttttcgctat ggaaaaaaac
     6781 gaacattctg gaagaaagtc tcttcggcag atggacagca actgatctca agatttaagt
     6841 atttgggaca gatggatatc ggagaaaaaa gaaaagctca gttaggtgca tttacttatt
     6901 ctttagaaaa acaggaagtt cgtctgcggt tgtcttctgt aggagattac cttcaacgag
     6961 agagtttagt gattcggcta ttatacggta cttttgaaac accccatcgt tgctttagtt
     7021 ctcgagattt tgcaactatt ttacagaaaa ccgtcagacg tggattatac cttttcagcg
     7081 gaccagtcgg atcaggcaag acttctctaa tgtatcggtt ggctttggaa gaaaaattgc
     7141 aagtgattac tatcgaagat cctgtagaga tagaagaacc gttttttctg cagctacaga
     7201 tcaatccgaa aatagcacag acttatgata cacttttgag actcgcatta cggcatcgtc
     7261 cagacttgct aatcattggc gagataagag atgaatcaac tgcacatgct gcggtaagag
     7321 cagcactaac agggcaccgg gtttttgcaa ccgtacatgc acgcaatctt gaaggtacat
     7381 tggtcagaat ccatgaactg acaaagaaaa aagaagagct atctgaatgt ttagctggtg
     7441 tcatttatca agaacttttg gaagatgcca atcaccatcc ttctgtttta tggagttatc
     7501 aatttatgga ggaagaatca gtaaaaaaat catgggagga aagttatgaa gaagctcagg
     7561 aaaatatttg gcagtcagaa ctattctaaa ggattaacaa gaagccagca aaaccagttt
     7621 gcacaaacca tgggagaact gcttttatct ggttttagtc tgcaagaatc cctttattta
     7681 ttactcacga tccgttcgtt tccacctgtt attttggcaa atatccaaca tcagctaaag
     7741 gaagggcaag cgtttagtga ggtactttcg caaataggat atggagcaga gcaactggta
     7801 cagatcgagt tagctgaacg tcatgggaat ctgccgacaa ctttgttgga aattgccaaa
     7861 cagtttcggc ttgttgaagg atttcgcaat gatttgaaaa agctgcttgc atatccgatg
     7921 ctattgctca cctttttgct aggtatcttg tttagtatgc ggttgttcgt tttgcctcag
     7981 cttattggtt caggaatggt catgcaagag cattggggta ttcagctgat acaagtcttt
     8041 cattggtatg ttctactatt catcctagcg gttactctat tggctttact agtaaggtat
     8101 cgtctgatga agttgtctcc tgttgatcag agtgaatgga taagcagaca attattgatt
     8161 ggaccgatat attccttgta tcaatcggct tatttgtcac tagagattgg aaagcttttt
     8221 catgaaggtt tcgaattaag gcaaatcata gtctgcttaa aggaaacgaa acaagaaagt
     8281 ctgattcagc atcttgcctc taagttgatt caaggattgg aggcaggtat gtcattggct
     8341 gagcagttcc agaattatcg ttttctatca gaagaattca gtaaaattgt tttacaggga
     8401 gaggcaaagg gaagtttagg taaagaactg attttttaca gcgaatggac aagaaaacag
     8461 cttttcaaac gtcttcatca ctggatgcat tttattcagc caatcatttt tttgggcgta
     8521 gcggctttga ttttattagt ttatgccgct gttttattac ctgtttacgg aagcatagag
     8581 gaggttttgc catgaaaaag agaaaaatat cttattatag cggttttaca ttgatcgaaa
     8641 tgctaatcgt cttactgatc atcagtgtat tagtgttgtt gttcgtccct aatttgtcgc
     8701 gttataggaa tcatgtagac caagaaagtc gtgaagcgat catccagttg gtagacacac
     8761 aaaaagagct ctatgcgctt caaaataatg gaagagttcc tactgtggag gaattgttga
     8821 atgaaggcta tatcaaacga gaacatgcag aaatctatca gcggccttga aggtaaggtt
     8881 ttagcatcag gatatacgtt gtttgaatca ctcttactga ttttattact tacaacgatc
     8941 ctttcttttc cagttctcgc attttctgca tggaagaatg aattggcagt ctatcaattt
     9001 ttcggccaat ttgaaaaacg aatctattct acgcaaaaaa tcgctattgt gaatcagatc
     9061 caaacaggct tttattggaa tagtgaagag aatcaaatca tttttcatgt tccgaatcca
     9121 gataaaataa attgggaagt attagatatt cctgatgaga ttaccttgaa acgccatgct
     9181 tctattactt ttgctgctag aaccgggaat gaaagttcac taaaagctta tcaattttat
     9241 tgggaagaaa aagagcggac gattacgtat caattccaga tagggagtgg tcgctataca
     9301 aaaagaattg aataaaacag gatttgtttt gttggagtcc cttacagcgc ttatgatcgc
     9361 ttctgtctgt gtctacttag tgagctttgg ccagctgttt ttgcttcagg cagaaaagaa
     9421 aagtggggag gaactgctat ttattcgaat gatttatgaa gatatcaaga cgcagcggat
     9481 gtatcacaaa agaccagagc gaagggaaga aatgatccca tacgatatcc aaattgtcga
     9541 taataatgga gtaatcgaaa aagtgtttgt caatatggag aaagaagcga tcagtattga
     9601 aaaaacaagt aattccagca tttacgctga ttgaatgtct aattggactg atgatattga
     9661 gcagtctatt gctcacattc aatcttttga ttcagcaaac catccaagta agtgaatttt
     9721 tgcagcgtaa agatcaaaaa gagtggctaa tatttttagc acaattagaa gaagaactca
     9781 agagcagtca caatgtctct gtgaaaaatc agcaactgca ctacacgatc gaggataaac
     9841 agtatatcat cgaaagatat caggcaatga tacgtaaaag aagaacttcc ggcggacatc
     9901 aacctatgtt aacaagtatc actgagcttc aattattgga aaaagaacac acaatttctt
     9961 tttatgtaca ctttgaaaat ggagaagatg gatatgccat atggacaaaa aacgatcagt
    10021 agaaaatgtt ataagggctc tattttattt tctgtcttgc ttttatttct tttatgtacg
    10081 tttttattga tggcagcatt agaaagctac cgtttgtcaa tggatcttaa tatacgaatc
    10141 aaaaattatt atatagcaaa aataatgaag cagatggctt tatcggaaca gtctacgctt
    10201 gccgaaagtt cggaaggagt tttttactac acaactggat ctgtaacata tcagtgggta
    10261 caacagtccc tattcttaga ggtggaagtc tcaccgttta tctttcggtt cacagaagaa
    10321 gtaaaaaatg atacagatac tagtacagaa tgatggaatg ttcgtgaaaa cgccctcttt
    10381 tctgttataa cactgttttt aataaaaaaa tgtttgccgt ttcacaaata aaaaacagtg
    10441 aaaacgattg aaattttgtc aattgttcga tagaattaaa caggtgaaaa aattgttata
    10501 atatatctag aaatgaggtg caccatgtta ccagaaaaaa tggaacaagg gtttaatcaa
    10561 aacctggaag ctattcagct tttacagaat gcattgggaa cttccttttt agaagcctat
    10621 gtcgaaaatg cagaaaattt aatagatgat tatcaagtac gtgtggtgga tggagtgccg
    10681 acaaaagaga caacgcaacg gatcacagct ctttatgaag agttgaagaa actttctttc
    10741 gagccagaag aatggcgcag actgtctcaa ttacttttat tgaagggcag ccagacagaa
    10801 catttgcagg cgaaccatca actcactcct gatagtatcg gttttttatt tgtttttctt
    10861 attgaacaac tatacacaaa taaaaaagca ccggttaaga ttctggatat tgcggcagga
    10921 atgggcaact tgttattaac agttctttta aacctatcga atgcggggta ccaaacagaa
    10981 ggaattggtg tggatattga tgatacattg ttggctgtcg cagcttctac aagtgatttg
    11041 acacaagcaa atgttcaata ctttcatcaa gacgggttgc aggaattatt aatagatcct
    11101 gtcgattttg caatcagcga tttgccaatc ggttattatc cgaatgacga aaaagcaaaa
    11161 gagtttttaa caagtaccga agaaggccat agttatgcac atcatttatt gctagagcaa
    11221 tcaatgaaat atgtgaagcc ggatggtttt ggtttgtttt tgatgccgag tggattttta
    11281 gaaacggatc aaagtgaaga aatcaaaaaa tggtttaaag aagaaggtta tcttcaagga
    11341 atgattcaat tgccagatga attgttccgt aataaacaat cgcaaaaaag tattttgatc
    11401 ttacagaaaa aaggaccaca agcacaacaa gtaaaagaag tcttgcttgt gaaacttgct
    11461 tcattaaaag aaccagagaa ggtgacggaa tttttcaacg agtttaaaaa ctggaaatca
    11521 tctgctctgt aatcattcaa cgagaaggag agtcatatgt ctaaaacaat tgcaatcaat
    11581 gctgggagtt caagtttgaa atggcaatta tatcaaatgc cagaagaaac cgtagttgcg
    11641 aaagggatcg tcgaacgtat cggtttacaa gattctattt ttaccatcaa gtacggcgat
    11701 aatcaaaaat tcgaacaaat catggatatc gaaaaccacg aagtagcagt aaaaatgctg
    11761 ttagatcagt tgattgaact gcaaatttta agctcttatg atgaaatcac cggagtaggg
    11821 caccgtgttg ttcacggagg agaaacttat ggggattcag tagtgatcga tgagaaagta
    11881 atggaccgca ttgctgaatt agcagaattt gctcctttgc ataatcctgc aaacttaatg
    11941 gggatcaaag cgtttaaaaa gattttacct gacattttga gtgtggctgt attcgataca
    12001 tctttccact caacaatgcc aaagcaaaat tatttatata gcttaccggt gtcttattat
    12061 gaagatttca aagtaagaaa atatggcttc catggcacaa gtcatcgtta tgtgtcggaa
    12121 cgtgcggcaa aattattgaa caaaccgatc gaagaactaa aaatcatcac ttgccactta
    12181 ggaaatggtg tatcgatcac agcagtcgat ggaggtaaat ccatggatac ttctatggga
    12241 ttcacaccat tagctggggt gacaatgggt acacgttcag gtgatatcga tccagctgtt
    12301 ctcccatatt taatggaaaa attagagatt gatattgatg aaatgatcaa tgttttgaac
    12361 aagaaatctg gtctgctagg cctaacagga ttgtctagtg atatgcgtga tcttgaaaag
    12421 aactatgaaa aagaacatat tcgtttagct tatgacattt tcgtggaccg catccgaaaa
    12481 tacatcggcg gttatgtgac aacgatgaat ggtgttgatg caattgtctt tactgctggt
    12541 atcggcgaga atgacggaca tgtacgtagt gaaatcatca aaggtatgtc ttggtttggt
    12601 tgtgaaatcg atccagcgtt gaatgaattg cgtggtgaag aagtagatat ctctacaaaa
    12661 gaatcaaaag taagagtatt agtgatccca acagatgagg aactaatgat tgcccgtgat
    12721 gtggagcgtc tgcgcggcta agccaaagca ttcgttagga taaaagatga aacgcatgca
    12781 agagggaatt cttgcatgtg ttttttttgc ggagaagacc gcaaaatggt aatttaactt
    12841 caatttccct tcttttttac aggatcttct tagggaattt tgcttggaaa agtggtcaaa
    12901 agggaaaaga ctggcatttt atttattgat tttcaggaaa atcccaatat tttattttca
    12961 ttcatcttta taagggacat tacatatgta aaaagaaaaa acatttttag atcagcctcg
    13021 tgggctgtcg actttattct tcgctttatg tttagctaga aaaccgatcc gaaacattca
    13081 ttgatcgaat aataaaaact tgattttcca cctgtacttt gcctgaatcg tgaaagcaca
    13141 ggtatgaaaa tcaagttttt tgttgcgaaa aaacgtccta gtcaatcagt tggccgagcg
    13201 gctggcggct gatctgctcc tgatcaataa cgagtgcttc ttggaagtgt tgctgcaaaa
    13261 agtagcggtc atggctgact gctaggagcg ttccttgata gtcttgcagc atttcttcaa
    13321 tttcttctcg agcatagctg tcgaggtgat tggttggttc atccaaaatc aggacgttga
    13381 ttttttctaa aaagagtttc agcaacgcca aacggatttt ttcaccaccg gataaatctt
    13441 tgatccgttt acccacatcg ctttgataaa agccactttt ggctaattgc cgtcgagcga
    13501 cttcttcgtt tccagtcaat gtgatggcgt aagccagcaa acggctatcg ggatcggcaa
    13561 actgcagctg ctgcggcaaa taaccgattt gcaagctact acctaatttg atggtgccac
    13621 cgtaaggctt ttcctcaccg agtaataatt tgagtaaggt tgattttcct gcaccattgt
    13681 cgccgatgat ggctaaatga tctccacgat agactgcaaa actgctgtct ttgaatagta
    13741 cttgatcagc gtactgctgg tgcaagtttt gaatgatgaa aacttctttg ccagattttc
    13801 caccattaga caagctttgc agccgatttt tcgggggagg tggcggtttg acgagtgtca
    13861 gtttcgccag gcggcgttcc agttctttgg cttttttgaa gaaggactca ttgtctcctt
    13921 catgtgccca ctgacgaaaa cgacgaatca tgactttcaa ccgctggact tctttttgct
    13981 gcagttcgta attttttgtc agctcagcca agcgagcttc tttcaagact cgaaaacggc
    14041 tgtagtttcc gggatactca atcaatgcac cttcttccag ttcccatgtt tttgtggtga
    14101 cttgatcgag aaattgccga tcatgagaaa cgaccagaac cgattttttt gtattctgta
    14161 aatagttttc caaccagcga atactgttaa gatctaaatg attggtcggt tcatcgagta
    14221 aaagtaaatc ggcatcggct gctaaaattt tggctaaagc gactttaact cgttcgcctc
    14281 cgctaagttc tgttaaccgt gcttcctgac ggtgtggaat ccctaagcct tgcaagatcg
    14341 ctgcaatccg gtcttctaat acgtaaccgc ctagttcttc aaagcgatgc tgcagctcac
    14401 cgtaacgggc cagacacttc tctaagtttg tatctggttc tgtcatcaat gcttcgtagc
    14461 gactcatttg ctgtttgagg tcctgtatct cagcggcaga atggagcaag tagtggttga
    14521 cggtcgcttc ggaaactgta agttcttgtt cgaccgtccc gatctttaag ccttttttgc
    14581 gactgataac accgctgtcg acaccttctc tgccggtcaa gatttgtaaa aaggttgatt
    14641 tgccagttcc gttgataccg actaaggcaa ttttttcacc agcttcaatt tgacaggata
    14701 aatcctcaaa taatggattc ccgccgattg tttttttaat agaaattgct tgtatgatca
    14761 tgatctctct ccttgagacc actcaaaaag gccatggtta tccatggcct gtgttattta
    14821 aactcagatt atcatgagaa aaataccttg agatggccat tgattttacg tgaattcaac
    14881 tgttttggac aaacttgtcc cgtcaacagc aactgtaaaa ccaattgcat ggctaaatgc
    14941 gatatggtat cgttggtaat tttcattact tgttatttta gtcatgcgca tcatagtatt
    15001 ttccctcctt cgtgtagttg tcttttccag tgtacctgaa agtgtcagaa agttcaacta
    15061 gcgctgacat ttacttaata tttatcgatg tgtaattctg tcagagagct ttttttatct
    15121 gactgagggt aatgtctcgt ggaccacatc attgtattct gagctaaacc agcgttcagg
    15181 cgaaatcata tggtgtttga taaaaaactt tgtctacatg acttcctttt gccatttgct
    15241 taataccgtt gcattaaaca gctcttctct ttggaaatct caccaacatc gcgttcagga
    15301 gtatcgttgt ggttgcaatg gcaagcggta gcctttcatc aattcttgaa tgatttaccg
    15361 aatgtagcac ttgccttctc gcggtacccg aacccgaaat aaaattttag tttggcgata
    15421 aaatcggtat ccgccatcga tacttcatat tttttcgtat cagattcatc ggaaacttcc
    15481 acattgacga accaataagc tttggcatgc ttaggtcgtt ttaaaccaca ccagggcgac
    15541 taatcgccct ggtgttcact tttttttatt ttacttgaac tcattgactg ttttcccttt
    15601 attttaaaca ttataaagac cgatgagtgc ttgccgcgtc ttttgttttt atcacggttt
    15661 tttgtgcaaa aaaacctgcc gatagttggc aggtcgtcta cgtgttaagc tttggtgtgg
    15721 tctttagctg gcaattggtt ttctaaatca gttcggacaa caagcacatc gcaagctgcg
    15781 tttctaataa catattcaga aacagaacca ataaataaac gttctacagc attcaatcct
    15841 gtggctccta acataatcaa atctacttga ttatcttctg ggatttctct ggcaataatt
    15901 tgttttggtg agccatattc tacgacagag gttacattat tcaagccagc tttcttcgca
    15961 ttgctttcat aatctgccaa tgtttgcttt gccatttcag tagcttgttc tgcaagcatt
    16021 ccatcaaagg atgaaacggt ttggaaagaa cgtgtgtcaa tgacatgtgc taatagaagt
    16081 tctccgttat ttcggatagc tacattgacc gcctttttaa aagctaattc agcttcatca
    16141 gaaccatcta cggcaaccat gatttttttg tattgttgta acataagcat tcactcctca
    16201 agtcaatcgt attgtactct tttattgtag aacactttag ggaaaaagag aattatttac
    16261 tgctttggtt cagcaatttt ttttgcaaat cggatactga aggtggcaga aacaataatt
    16321 acgatcagta aaaagaccaa ggcaataaag cgttgattga aaaaagctgt taaaataccc
    16381 attacgccta aaacagcata tgcagctccg taaaattgta ggaatccttg atttttatca
    16441 tttttgttga tcaatacaaa aaagatatcc tgtttattaa ataaataata gctaacgaat
    16501 aataatacta aagtggcgat aatcattaaa atcctaatca tgaacaaatc ctccaaaata
    16561 agcaacaaat agaaaaagca atttgccaag agcaaattgc tttattcttt aaaagatggt
    16621 aatctccgtt aatgccgttg tttgtcctgt agtattgatc ccgcgaattc aagcaggtgg
    16681 gtcccatggt cgtagcttcg aactaccaaa tgataaggca tgttaccagc agtgacacaa
    16741 gacgggtccc aagatatcaa taaaaatgtt cggtcaacac acaaagagga caattcgaac
    16801 atcacagatt tcccacctct atagtagcat atgaatttaa aaagagcaat gagagagctc
    16861 gcagaaacag aaccgttttc ttgtctagtt ttttggagga tgctttttcc actcttttat
    16921 acggtaatac tgttgaccca gagcctgttc atatttccct gtatcttttg gctgataata
    16981 ctgagcattc ttcagtttat ctggtaaata ttgctgattg acccaagcct gatcaaaatg
    17041 atgaggatac tgataaccca cgccacggtt gagtgatttc gcacctttgt aatggctatc
    17101 tctcaaatga tcaggaacat ctccagcttt gccctctcga atatcagcaa gtgctgcatc
    17161 taatgccatg taggcagaat tggattttgg cgacaagcaa agatcgacga cgacgtctgc
    17221 tagtgggata cgtgcctcag gcaatcccaa tttttcagca gctaatacag cgttcactgt
    17281 acgagccgca gcagcaggat tacctaaacc tatatcttca taaccgataa ccatcagacg
    17341 ccggcaaata cttgccagat cacctgcctc aactaaacgg gcgagataat gaagtgcagc
    17401 atccacatca cttccacgaa tcgatttttg aaaagccgaa ataacgtcat aatgagcatc
    17461 tccatttttg tcatgggtaa gagcttttct ttggacacat tcttcaatga tcgacagtgt
    17521 aagatgaatc cttccttctt tatctgaagg agtcgatagt gtagccagtt ccagcccatt
    17581 caatgcactc cgcaaatcgc cgttcgttgc acgcgaaagg tgaagaagag catcttcgtc
    17641 taactggatt gcttgttgtc caaggccgcg ttctttatcc cttaaagcat gttcgactgc
    17701 caactggatg tcttgttctg ttaatggttt gacttcaaag atctgtgtac ggctgcggat
    17761 cgctggattg atggtaatat aaggattctc agtagtcgct ccgatcaaga tgattcggcc
    17821 actttctaaa tgaggaagca gaaaatcctg ttttgtttta tctaatcggt gtacttcatc
    17881 caataagagg atcactgtac cactcatttt tgcttcttcc gctacaactt gtagatcctt
    17941 ttttgaatct gtagcggcgt ttagcatgcg aaatgcataa ttagtagagc cggcgatcgc
    18001 acttgcgata ctcgtttttc cagtaccagg aggtccatat agaatcatgg aagagagcat
    18061 gcgagcatcg accatgcggc gtatgatttt tccttccccg actagatgct gctgaccaac
    18121 gacttcatcg atcgttcgag gtctcatgcg ataagccaat ggctgttgca taggattttc
    18181 ctccttcttg tttttacgtc ccttaattat atcatattta gcaagatgcc tagaaagaac
    18241 agccttctgt tgttttgttt tcatagctta tccaactaat attgtgtatg ataaagtagg
    18301 tgagaaaaat gacggataaa caaaagatag atgaattttt ttggaataaa acgacgccag
    18361 aagtggcacg ggatttattg gggatgtatt tggagcatga cacaccagaa ggaagattag
    18421 ctggatatat tgtagacgcg gaagcatacc taggtccaga agacgaggcc gcccatagct
    18481 atggtttaag aagaacaccg cgtgtgcggg caatgtatga aaaaccaggt acgatctacc
    18541 tttacacgat gcatactcat cggattttga acatcattac tcagccagaa gggattcctc
    18601 aaggagtaat gatacgtgca atcgaacccg ccgcaatgat tgatcaaatg agtaaaaatc
    18661 gtggcggaaa aactggtcca gatatcagca acggtccggg aaaattagtg gaagcattag
    18721 caattcctca agaactttat ggtcagtcta tagcagatag ttcgttacgc ttagtttttg
    18781 agaaaaaaaa gacaccaaaa aagatcaatg ctttgcctag aatcggtatc ccaaacaaag
    18841 gtgtatggac agaaaagccg ttgcgttttg ttgtgtcggg aaatccttat atctctttac
    18901 aaagaaaaaa ccaaatcgag aaagattggg gttggaggaa agaaaatgaa aaagaaggat
    18961 ctattaacat atttagatga aataattgaa aaacaagtga cagactatga tgtagcttta
    19021 gattgggata caaaaaatca tacgatcgag atcgttgttc gcttgtttgc agaaaataaa
    19081 gcacaatttg agattgatga tgcagaaggg atcgtttcgc aagaaccaat tatcgaattt
    19141 gaagacggcg tacttctttt taatcctcag aaatctgttt ttgatgaacg caattatcta
    19201 gcagtaatcc cttatgaagg aaaaaaaggg ttggcaaaat cagtagctga ttctttagtg
    19261 gaatatctaa atgaagtatt agctcaaggg caaagcgatt tgcttgattt tttggatgaa
    19321 gacgatgacg aagctgtatt cgagcttcac tgggataatc agcaactagc agaattagta
    19381 gaagtaaaac agcaaaatga agcagatact tatttaccgt acccaagtta ttaaggagtg
    19441 acagatatga aatggaatga agtaaaagta gaaactgaaa gtgaagcagt cgaagcagtt
    19501 gctaatattt tgatggaggc aggggcgagt ggtgtcgcga ttgaagattc tttagatgtt
    19561 gaacattttc agtcggatca atatggtgag attttagata aaaatgattt tacatcgctg
    19621 aaagaaggcg cactagtcat ggcttatttt ccggaaacga ctttcttacc ggaaatcttg
    19681 ccttttatca aagatcgtat cacacaatta cctgagtttg gtctagctat tggaaaaaat
    19741 aaagtaacag ttagtgaagt agaggaaagc aactgggcaa cagcctggaa gaaatactat
    19801 catccggtgc gtattacgcg aatgctgacg gttgcgccaa gctgggaaaa ctaccagaca
    19861 gaagatcctc tagaaaaaat tatcacacta gatcctggta tggcatttgg cacaggcact
    19921 catcccacga cttccttgac attgacagca ctagaaacag tcttgcgtgg tggtgaaaca
    19981 ttgctagatg tgggaactgg gtcgggcgtg ctaagtattg cagcaaaata tcttggtgca
    20041 aaagatgtct atgcctttga tttagacgaa gtagccgttc gttcggcgaa agaaaatatg
    20101 gatatgaatg aaatagccaa agatgtccat gtatcagcta acgatctttt gacaggaatc
    20161 gagattgaaa gtgatgtaat cgttgcgaac attttagcag atatcatctt attgatgatc
    20221 ccagatgctt ggcgtttact gaagcaaaca ggcacgctga tcgtttcagg aatcattgaa
    20281 gagaagaagc aaatggttct ggatgccatg ttagaacaag ggtttatcgt ggatcaaatt
    20341 ttccagcaaa aagactggta tgcaattatt ttgaaaaaac cagaggtgga atagatgcag
    20401 cggtattttt taaaagaatc atatgaatca aaagactatt ttgttttaga aggggaaaac
    20461 tatcaccaca ttgtacgggt tatgaggatg gaacccgagg atgaagtttt tttggcattc
    20521 aatgatcaaa tagccattgt tgcagaaatc acggagatta ctgaagaggc tgtttatctg
    20581 aaagaaatta aaaaagaaga aaatcaaaag gaactgccaa gttcgattac catagcgagt
    20641 ggctatccta aaggggaaaa gctggattgg atcgttcaga aatccactga attaggagcg
    20701 caagcttttc ttggtttccc ggcatcagcc tctgtcgtta aatgggatgc aaaaaaacgt
    20761 atgaaacgac aacaacggct tgaaaagatt gctcaagagg cagcggaaca atcccatcgg
    20821 cagatcgtgc catcagtaga gttattggaa acgaaacaag atttatttga gcggctagat
    20881 gattatgacg tggtcttggt tgcttatgag gaatcagcaa agcaaggcga aaaatcaaat
    20941 gcagccaaaa ttcttagtga aacaaaacca gatagtcgaa tcttagctgt ctttggacca
    21001 gaaggtggat tatcccgcga tgaagtcgat tattttgtag aacacaaagg gatactttgt
    21061 ggattaggac caagaatcct tcgtacagaa acagcacctc tttatttatt aagtgctgct
    21121 agctatcaat gggaattaat gaatccttct ttcagctaac gttcttcatt aataaaagag
    21181 tatcgacaga aagaattgaa aaaatagtca acaatttgta taatagaaac aattatttat
    21241 acaatggata cgaaaaatcg tttgaaaaag cttttaaaaa gcgaacattt ccgtaaaaaa
    21301 acgtataact gcacggattt tgggacaatt atcaaacaga tatttgtccc gattcccgtt
    21361 ttaccgaaaa aagaaagggg gatcgattcc tgtgacaaaa gaagaaattt taacgggacc
    21421 cggtgtcata aagctcgtta gtcaatacat gggtcccgaa catgtagcat ttgttgagaa
    21481 agcatgcgaa tacgcgactg ctgcacatga cggacagttt agaaaatcag gcgaacccta
    21541 cattattcat cctatccaag tcgcaggtat attagcagat ttaaaaatgg atccccatac
    21601 agtggctaca ggcttcttac atgatgttgt tgaagataca gaaatcactt tagaagatct
    21661 gagagaagaa tttggcgatg acgttgctat gttagtggac ggcgtaacca aattagggaa
    21721 aataaaatat aaatcccacg aagagcagct agcagaaaac caccgaaaga tgctgctagc
    21781 aatggctcaa gatttacgag ttatcatggt caaattagct gatagactac ataacatgcg
    21841 tacgttgaag cacctgcgag aagataagca aagaaggatc gctcaggaaa ctttagaaat
    21901 ctatgcgcct cttgcacatc gtctagggat cagccggatc aaatgggaat tagaagatac
    21961 agcacttcgt tatctaaatc caaaacagta ttaccgtatc gtccacttga tgcagacgaa
    22021 gagagaagaa cgtgaaaaat acgtaagtgg tactgttgaa gatattcgaa tagcgacaga
    22081 agagctgggg atttttgcag aaatctatgg acggccaaaa cacatttatt caatctatcg
    22141 taaaatgaaa gatcagaaaa agcagttcaa cgaaatttat gacttgctag ctattcgagt
    22201 gatcgtagat tcaatcaagg attgttatgc tgtattagga gcaatccaca caaaatggaa
    22261 accaatgccc ggcagattta aggattatat tgccatgcca aaagctaata tgtaccaatc
    22321 tttgcatact accgtgatcg gtccggcagg aaatccggta gaaattcaaa tccgaacaca
    22381 ggaaatgcat gaaatcgctg aattcggggt tgctgcacac tgggcctata aagaaggaaa
    22441 aaatgaaaaa gtagaaccag atggtatgac gaaacaatta agctggttcc atgagatact
    22501 cgaacttcaa gacgaaagct atgatgcttc tgaatttatg gaaggcgtaa aaggagatat
    22561 ctttagtgat aaagtctacg tcttcacacc aaaaggagac gttactgagt taccaaaagg
    22621 atccggacca ttggactttg catacagtat ccatacagat atcggtaaca aaaccactgg
    22681 tgcaaaagta aatggcaaaa tggtgcagct tgattacaaa ttgaaaaacg gagatatcat
    22741 tgagatcatg acttctccaa attcatttgg cccaagtcgc gactggttga aattagttgc
    22801 tactagcaaa gcaagaaata agatcaaacg tttcttcaaa gcccaagatc gagaagaaaa
    22861 tgtgatcaaa ggccacgaat ccgtggtcaa atgtattaca gatctaggat ttacgcctaa
    22921 agatattttg acgaagaaca aactgcaaga agcactcgat cgttttaatt atcaaacaga
    22981 agatgatctc tatgcagctg tagggtatgg agaagttagc cccttgacga tggccaatcg
    23041 tctgactgaa aaagaacgta aagaacaaaa aatcgagcag caaaagcaag aagcagaaga
    23101 aatcatgaat cagccgaaaa aagaacctga caaaatgaaa gtacgtcatg aaggtggcgt
    23161 tgtcattcaa ggggtagaaa acttactaat tcgtatcagt cgctgctgta atccgattcc
    23221 tggtgatgat atcgttggtt atatcactaa aggcagaggg atatccattc atcgtcgaga
    23281 ttgtccgaat gttcagcctg acaaaccaaa tgtagcagaa cgtttgattg aagtcgaatg
    23341 ggaagataca tcgaatacac gaaaagagta tgatgcagat ttggaaattt acggctataa
    23401 tcgttcaggc ttattgaatg atgtacttca aacagtcaat gcgctaacga aaaatctcaa
    23461 cagcgttgaa gcacggacga ataaagataa aatggcgacg atccatttga cggttggtat
    23521 ccagaattta tcccacctaa agagtatcgt ggataaaatc aaagcagtac ctgatgtcta
    23581 cagtgtacgc cggacgaatg gataggagga cagatatgag agcagttgtt caacgggtaa
    23641 gtaaagctgc tgtgtcagtc gatcagcaag aaattggaaa aattggctac ggcctacttg
    23701 ttttattagg cattcatgaa accgatacgc aaaaagatgt ggactatctg gtaaaaaaaa
    23761 ttgcacaaat gcgtattttt gaagatgaac aaggaaaaat gaatattagt gtagaagatg
    23821 tgggaggaca aattttaagt atctctcaat tcacactgtt tgcggatacg aaaaaaggaa
    23881 atcgaccaag ctttgtggaa gccgcacgac cagaaacagc tattccttta tacgaatcat
    23941 tcaatgaagg aatcagaagt cgcggcatca cagtcgctac cggacaattc ggagcagata
    24001 tgtcggtttc tctcgtcaat gatggacctg taacgatcat tattgattct aaaaacaaat
    24061 aaagtaatct ggttttcact agtaaaaact atgaaacacg tcgcttaagt tagactgata
    24121 aactctaact taagtggctt ttttgtctgc aaaaagcgct tcttttgtca gcagtcggaa
    24181 aaaaagttaa gcttaaatta tacaagttat tttttatagg aggttgctga agatgaaaga
    24241 agtaccagta gtagatatca aaatcacaga tttaaaaaaa ttgtatcaaa aaggatacaa
    24301 catgttggag gagttacgcc atgaagcgga cgctcccgtc gtcaaagcaa agattttcaa
    24361 taaagaagct atcacgatct acggatcatc agcagcaaaa gtattttatg atccaaggaa
    24421 tttcaaacga aaaggtgcta tgccaaaatt agtattgaag acactttttg gtcaaggtgg
    24481 agtacagact ttggatggag ctgctcatca ccatcggaaa aatattttta tggatctaat
    24541 gacaccggaa agaatggaag attatcatcg tatcttagat aagaatctga cacaagcact
    24601 tgaagcacag catggtcaat ttgaactatt tgatttaagt aagatggttt tctttacatc
    24661 tatttgcgag tgggcaggta tcaatttatc agcgatttct aaagatgagg tcgaaaaatt
    24721 agcagaatat caaatctcta tgatcagcgg aacgtttact tctccgattg atcacataaa
    24781 aggtgtcgaa aatcgcaaaa aatcagaaaa atgggcgcaa ggcttgattg aagaagctcg
    24841 acaaaatcct gtggctggaa aagaaaatgt ggcattatat gcttttgcca atgccacaga
    24901 tttagatggt cagcttttac ctctggaagt ggcagctgtt gaattattga acatcatccg
    24961 tccaacggtt gctctaactg tgtgggcggc attgatggga catgcacttt tcagtagacc
    25021 agatttgtat cagcaattaa aaaatgactt ttcgacgtta caagatccgt ttattcaaga
    25081 aatgagacgc tattatcctt tcttcccaat gctacctgcg atttcgctga aagaagtaga
    25141 agtagatggt tatcgtatac cagaaggcag ttgggttatt ttagatttat atggaacaga
    25201 ccatgatgaa cgaacagttg aagcgccaga ttcctttatg atcaaacggt atgtagggaa
    25261 agcaaaagat atttcctata aagaagaata tgagatgatt gcacaaggcg ggggaaattt
    25321 tcgacaaatg catcgctgtg caggagaatg gatcactctt catagtctac gtgtgttttc
    25381 cgatcagcta gtaaataagt ttgagttctc tgtacctgaa caagattgga cgattccatt
    25441 caatcagttt ccgacttatc cgaatagccg agcgctactt tacaagaatt agattagcat
    25501 aaacttatcg aaaaaaagga agatcaaaca gtgctgatct tccttttttc atattactat
    25561 aaattgataa ggtcaattga tcaaaatcca cgaaatgctt catcaatggt ttgataatct
    25621 tcattggata aatgcaaatc taaagcttgg acattgtcag atacttgctc tggacgctta
    25681 gctccaggta taactacaga cactcgtgga ttcttcatgt accaagctaa aacaagttgc
    25741 gcaggagtgg cttggtatct tttagcaatg gggcgaagta catcgacagc ggttaaaatt
    25801 tctctgaaac gttctccttg gaaatctgga tttctttttc gaggatctcc ttcaccaaat
    25861 tgtttttcct caccaagttc atattttcct gtcagcaaac cggaagcaag aggaaaatag
    25921 ggaacaaaac tgattttgtt tttttcaaga taagggaata attctttttc agcctgccga
    25981 tggatcaaac tgtatttatc ttctactaca tcgacatatc catctgcatt cgcttctttc
    26041 aattgttcta acgtgaagtt tgacacaccg actgctcgga tttttcctgc ttcttttaat
    26101 tcgtgcaatg tagcaactga ttcatttttt ggtgtgcttt catccggaaa gtggatataa
    26161 aaaatgtcga tgtaatcagt ttgcagacgt tttagtgcat cttccaccga ttgtttgaga
    26221 aactcaggag aattatcaaa tgttcgccct ttatttggta catgagcagc ttttgtagcg
    26281 atgacgacac gtgagcgatc atattctttt tccttcagta cctcgccaat cagttcttcg
    26341 gaacggccgt ttccgtaagc ataagcggta tcgatcaaat tgatcccgct attcaaagct
    26401 gtacgaacta cttgttttcc tgtctcatct tccaaaccgt caaaaagatt atgtcctcca
    26461 acggcatttg ctcctaatcc aagctgttct gcgtagactt gtgaatgtcc gattcttatt
    26521 tctgccatag ctgattcctc ctttttgtct agtctaaaac aaaataccaa aaagctgaac
    26581 taaaacgttt ttgtgagggg aaatagtcag atatctcttg actttgagac tggtttttag
    26641 taggttataa gtaatatcga atgtcaatga aagagcgagt aagtttttga aactttacaa
    26701 gagaggatca tcgaggctga aaatgatcta aagtttcatt agcctaaaga cactctggag
    26761 atggacgagt gaaaagccgt cccgcgtacg agcgttattc gtaagaggaa agagaaatct
    26821 ttcaaaatta ggtggtaccg tgtatgaatt tcattcaccc ttgttcatga tgaacaaggg
    26881 tgattttttt atttacatag ctaagaaagg gagagaaaac tatgagtttc caaagaccaa
    26941 aaggaacaaa tgatattttg ccaggcgaat cagaaaaatg gcagtttgtc gaagaaacag
    27001 cacgtttatt attcagagat tatcagtata atgaaatgcg tacaccgatt tttgaacact
    27061 atgaagtgat atcccgaagt gtaggggata caacggatat cgtttcaaaa gagatgtatg
    27121 atttttatga caaaggggaa cgccatgtga cgcttcgacc agaaggtaca gcaccaatcg
    27181 ttcgttcttt tgttgagcat aaattgttcg gcccagagta tgcgaaaccg ttcaaaactt
    27241 tttatatggg accaatgttt cgctacgaac gccctcaaaa aggacggtta cgtcaattcc
    27301 atcaaatcgg agtagaagca tttggatcag aaaaccctgc aactgatgtg gagagcatgg
    27361 tgatggcact tgatttcttc aaacagttgg ggatcacgca aatccggcta gtcatcaatt
    27421 cattaggtga taaagaaacg cgtcaagcct atcgtcaagc attgatcgac tatcttttac
    27481 cttttgaatc agagctgagt gaagactcta aacgccgcct tcatgaaaat ccattacgcg
    27541 tattggacag taaagacaaa cgagatcaaa aatttgtggc agaagctcca tctatcttag
    27601 attatctaag cgagcctgct caaaaacatt ttgataccgt tgtttccatg ctggatgcat
    27661 tggctgttcc ttatgaaatc gacagtaata tggttcgcgg actagattat tacacacata
    27721 cgatttttga aatcatgagt gacgcaccaa aaatgggggc acaagcaacg atttgcgcag
    27781 gcggacgtta tgacaatcta gtggaagaac taggtggtcc ttcaacacct ggcttcggat
    27841 ttgcaatggg tatcgagcga ttactcttga ctatggaggc agaggaagta gttatccctg
    27901 catttaatga gttggatgct tacgtggtcg cactcggaga cgaaacaaat atcgaagcat
    27961 tgaaagtggt ccaagcgatt cgaaactttg gtttttcagc tgaccgcgac ttcatgaacc
    28021 gtaaagcaaa agcgcaattt aagacagcag ataaagcgaa tgccaaatta gtattggtga
    28081 tcggtgaaga tgagcttgct agtcaaacga ttaatgtcaa agcaatggca agccgaaaag
    28141 aaaaaagttt tccattagaa gagatatatg aacattttga tgaagtctat gatgaaatga
    28201 ctttattcga gtaggaggaa aaaaataaat ggcacaaaga acgatctatt gcggattagt
    28261 ttccgaacaa caattagaac aaacagttac attaaaagga tgggtacaaa aaagacgaga
    28321 ccttggggga gtaatcttta tcgacctacg tgatcgtgaa ggaatcgttc aaatcgtctt
    28381 taatcctgaa atcaacaaag aagcatggga aatcgcggat aaatgtcgca gtgaatacgt
    28441 gatcgaagtg actggggaag ttagaagacg cggagaaaat gcagtaaatc ctaaaatggc
    28501 cactggtgaa ttcgaagtga ttgcaagtga aatcgtgatt ttgaatcaag caaaaacccc
    28561 gccatttcaa attgaagatg atcaagcaat cagtgacgaa atacgtttga aatatcgtta
    28621 tttggatctg cgtcgtccaa aaatgactaa aaacatcaaa ttaagaaatg cgacaacaaa
    28681 agcgattcgt cattatttag atgataatga ctttctcgat atcgaaacac cttacctagg
    28741 gaaatctaca ccagaaggcg caagagacta cctagttcct tcacgtgtcc atgctggaca
    28801 tttctatgcg ttgccgcaat ctcctcaatt gtttaaacaa ttgctgatga atgctggatt
    28861 cgaccgttat tatcaaatcg ttcgttgttt ccgcgatgaa gatttacgtg gagaccgtca
    28921 gcctgaattt acgcaagtag atattgaaac aacgttctta acagcagaag aaatccaaac
    28981 ttacacagag ggattgattg cgaaggtaat gaaagaagtt cgcggtatcg aagtcacatt
    29041 gccatttcct cgtatgacct atgatgaagc aatggctcgc tacggcagcg acaaaccgga
    29101 tacacgtttt gatatggaat tgatcgatct aagcgataca gtcaaagaag tagaattcaa
    29161 agtcttccaa atggctcttg aaaacggcgg tgtcgtgaaa gcattgaatg ctaaaggcgc
    29221 tgcagatcgc tactcacgaa aagacatgga tcagttaggt caatatgtag gacaattcgg
    29281 agctaagggg cttgcatggc tgaaagtaga agaagacgga ttgaaaggtc caattgctaa
    29341 attcatggga gaagcaacag aagcaatcat taaagcaaca gatgcaaaac caggtgactt
    29401 gctgatgttt ggcgcagata aaagtgaaat cgtcgctgct gcattaggtg ctatccggac
    29461 acgtttagga aaagaattag gactaattga tgaatcgaaa ttcaatttct tatgggtaac
    29521 tgattggcca caatttgaat tttcagaaga agaaggtcga tatgtctctg ctcaccatcc
    29581 attcacgatg ccaaaagaag aagatatccc gttattggct gaagaccctg caaaagtcta
    29641 tgcagaagct tatgatatcg tcttgaacgg ttatgaatta ggcggaggtt ctatccgtat
    29701 tcatactcgt gagcttcaag aaaaagtact agaaactcta ggctttacaa aagaacaaat
    29761 ggaagaacaa tttggtttct tgttaacagc tttagattac ggattccctc cacatggcgg
    29821 tattgcatta ggattggacc gttttgcaat gttattagcc ggagaagata atattcgtga
    29881 agtcattgcc tttcctaaaa acggaaaagc agcagatgtg atgagtgatg caccaagtat
    29941 ggtttcgcct ttacaattgt tcgaattgaa tattgatgta acgaatatag aagaatagcc
    30001 gataaaaagg taaaaaggat ttgtcgaata cgcttctttt gacaaccttg atttaattga
    30061 aaaacacttt ataatgaagc cataacatat aaagcggggc ttttccatga aaaaaacatt
    30121 cgaaaattgg gcttatcatg attacacgac caagctttcg gtatctatcg tttatgcgtt
    30181 gatcgcatca gtcgcattga atttctttta tcagccagga catatttatt caagtggtat
    30241 tactggtcta gctcagattt tgacgacttt gtcaaaatca ctgataggat tcgaagtacc
    30301 ggtatcaaca actttgtatt tactgaatat acctttgttt gtattggcgt ggttaaaaat
    30361 cgggaagaag tttacaattt tcacttttat cacagtcact ttgacctccc tttgtatcca
    30421 tattttaccg gaaacagtcc tatctaccga tccaatcatc tgtgcgttgt ttgggggggc
    30481 tgtgatggga ctaggtattg ggctagcgtt gagaaacggc ctatcatcgg gcggtctaga
    30541 tatcgtgagt atcacgattc gtaaaaaaac aggaaagtcg attggttcta tttcgatcta
    30601 cttcaatgca ttgatcgttt ttgttgccgg aattctattt ggctggcaat atatgtttta
    30661 cagtacgttg tctattttcg tcagtggaaa agtaactgat gtcgtttata ctaaacaaaa
    30721 gaagatgcag gtaatgatcg taacgaaaca tccagaaaaa gtagtaaatg aaatccaaaa
    30781 ctgtatgcgt agagggatca cgatcatcaa tgaagcagaa ggtgcttata agcatgataa
    30841 gcagacagta ttgatgacga tcgttacacg atacgaactg cctctactta gagaagcaat
    30901 gaaagcttct gatccacaag cttttgtaag tattgcagaa aacgtccaga tacttggtcg
    30961 tttctatgag gatggcattt agatcgagtt tgacaaaaat tcatgaaata ctgctgtaga
    31021 cagacgttcg tttgtctgca gtttttttct accatagtag aatcagcaaa gaaaaactgc
    31081 ttttttattt gaaattttct taaagttgaa aaaaagcatg tttatttttt taaatttatg
    31141 ggatgataga aaaaaatagt agagaaagga cacgattcta cttgctattt tcacttaata
    31201 cccgtatatt acgaatgaaa aaagtttatg aaaagttgga atattgaatg gaaaaacaaa
    31261 aaaggttaaa aaacagccgc tacatcactg ggtttgatgg gctccgttca ttagctgtgg
    31321 tgggtgttat cttctatcac cttctgccaa ctagtatgaa aggcggctat ttgggcgttc
    31381 cgattttttt cgtcgtatct ggttatttga tcacggatct gctgcggcag gaatgggaac
    31441 aaaatggaaa aatcaatatt tggcaatttt atgtcagaag aatgaagaga ctctatccgg
    31501 cacttgtttt tatgcttgtg acagcttcgg cttatatcac tttgtttcag cgagggctgt
    31561 taaacaatct tcgaggagtt gtggtcagca gcttactgta tgtcaacaac tggtggcaaa
    31621 tcaataacgg tttatcttat ttcgatcgat ttgctaatga gtcaccgttt acgcatatct
    31681 ggtctttagc agtagaaggt caaaactatt tggtttggcc gatcttgttt gtattgttga
    31741 tggtatttgt tcgtaaaaga aaatggatct tttatactgt gttaggtaca tccttcctct
    31801 ccgccatctt gatgatgatc ttgtatacac caggaggcga tccaacccgt gtttattacg
    31861 gtaccgacac tcgtctgttt tctatatggt tgggaagtgc gcttgcgttt gtttggccaa
    31921 gtacgcgttt aaagaaaaac atcccgaaac aagcgaaaaa tattctgaat atcgctggag
    31981 gaatctcgct tttggcactg attctttgct tcttcttttt agatgatcat tacaatttca
    32041 tctattacgg aggaatgctc ctagtgagtc tgttatgcgt agtgctagtt gcagtgacag
    32101 cacaccccgg agcaagtttg aatcgatggc tgacaaatcc agtatttact tggattggca
    32161 aacgaagcta tggcatttat ctgtatcagt ttccagttat gattttttat gaagcaaagg
    32221 tgactgatat tgccgatcat gttttgttgc atactttagt ggaaatcaca ttgattctaa
    32281 tcatcagtga actttcttat cgatatgtgg agcgtccgct agcacgtttt gattatggtc
    32341 gtacattcca agtgatgaaa ggatggttca gcaaaccgat catcagtaag agcaaaccat
    32401 gggttgttcc aggcacatta cttgtagcaa ttgcactcgt tggtttcgtt acggcaccga
    32461 aaaacgctgt aacagccgat caaaaacaac tgcaggcaaa aatcgctgaa agtaaaaaat
    32521 tagcagaaga aaccagaaac aattcagaaa acaccactag ttcggttgac caagcgatcc
    32581 ttgataagta cgagttgtcc aaggcacaag ggaataaggc acaaaaatta gaactaacgg
    32641 catttggtga ttcagttatg ttggatgctg caacggatct acaagaactt tatccaaaag
    32701 tagtggtaga tggagatgtc ggacgacaat tatatgccag tgtaccgtac attgaaaaat
    32761 taaaggaaca agatttgttg aaagacactg ttctgattgg actaggaacg aatggctcat
    32821 tctcggaagc tcagtttgat agtgtgatgg atgcaatcgg tgatcgaaaa gtatactggg
    32881 tcaatgtccg agtaccgacg aagcgctggc aaaatgacgt gaatgcgatg cttgaacaga
    32941 tgacgaaaaa atacgacaat atgacgctga ttgattggta cagctacagt aatgaccata
    33001 gtgactggtt ttatgatgat caagtccatc caaatcctga agggatgaca cattacattc
    33061 acttagtcag cgaagccttg ctaggaggaa gttcaagtac gaacagcagt gagacaaacg
    33121 tgacagaatg atagctacaa aaaacttcag gcaaaaacat ttgtttttgc ctgaagtttt
    33181 tttatattta ccgtgttttt aacattcata tagattcagg aagcaaaacg gttgagtgtg
    33241 tctgtaaata acgttctttt tgttttcggg tgagtttttt gaatgcagct tctgcttttg
    33301 ttgcttcgct tcttgaagaa aatacttctg catggatcat cttaacaggt cttctttttt
    33361 gaagacgagt atatttagca ccagtaccat tgttatgttc ctctaatcgt ctagaaagat
    33421 cggttgtata tccaccataa aatgtctgat ctttacagaa taatacgtaa aaataatgtt
    33481 catttgccat agagcatctc acgaatctct gcagtgtatt ctccatccat attgtaagtg
    33541 atcaatggtg gagcgacttt gaatccgtct aatttgcctt ctttgattcc ttcaactaac
    33601 aaaatatttg cctcttttcc aggttttggg taaacaaatt ggatcctttt aggtgcaatt
    33661 cgatagtctt ccatgacctg aatgatttct aagaaccgat caggacgatg gaccatagta
    33721 aagcgtccat ttgtttttag tagcttactt gaaatatcga tcacttcttc taacgtcgta
    33781 tgaatctcat gacgggcgat tgccaggtgt ttattagggt tcgttttatt tgttggcagc
    33841 cctttgaaat aaggtggatt acagacgact aagtcacaag aattatgtcg aacgacagat
    33901 aagctgtctt tcagatcgat ggtgtggaca gtcatttgtt catttaaatg gttaagcgcg
    33961 atactacgtc ttgccatgtc tgctagacga ggttgaagtt cgatagcgtc gatagccgcc
    34021 tgggttcgtt tacttaaaaa aagtccaact gcgccatttc ctgcacaaag gtcaacaatc
    34081 ttcccttttt taggtacacg ggaaaagtga gcaagcagca cagcatctaa agaaaaagaa
    34141 aacactttgg aactttggat aatcttgata tcttctgcgt ataattggtc gattcgttcg
    34201 tcttcgtgaa gcatggattc cctccaaaat gaaaaaaata gtcgtctgta ttatactatt
    34261 tttattaaaa agaaccaagg tggcttgtca aacctctatt ttttatgcat actatgacca
    34321 taagaaggga aaggaagaga ctatgtttta tcgatttatg cgcggggtag ttcgagtact
    34381 attagcctta gttaatggaa atgcccgcta tgagaataaa gaagttttac cgcaagatga
    34441 gaattacata ctagttgctc cccatcgtac ttggtgggac ccattatatc tggcggtagc
    34501 tgcagcaccc aaggaattca gctttatggc aaaagaagaa ctgttcaaaa atccagttct
    34561 gcgatttata ttaactcgat ccaatgcttt tccagttaaa agagaaaatc caggaccaag
    34621 cgtcattaaa acacctgtaa agattttgaa atcgacaaat ttgagtttga tcatgtttcc
    34681 cagtgggaca agacatgcta cagaacttaa aggcggaatg gcattgattt ccagaatgtc
    34741 taaagcgaaa atcgtgccag ctgtatatca aggtccgttg acacttgggg atctgttcaa
    34801 gcgtcagaga gtgacagtgc gtttcggcga accaatcgac ttatcagata tcaagaaaac
    34861 ggataaagaa ggtatggcgg agatcgaacg acgaacacaa gcagcttttg acaaattgga
    34921 caaagaagtg aaccctgatt tcaaatatga agtcaaaaaa aaataaaatg ataagtttat
    34981 gcgtgggcgg tcttttgaat ctactgtcct gttcagttca gactttttca atgaaaacga
    35041 gggagagacg tcaagtctgc cctctttttg cgttccaata tgtaaaatag aagacaggtg
    35101 tcatgtaatg gatcaaagtg ttataataat tagaaggaca ttttttgagg tgattgaatg
    35161 cgttttttac atacagccga ttggcatatc ggaaaaaaac tacaaggtta tgacttgatt
    35221 gaagaccagc gtcatgtact atcttctata ctagatatcg cgaaagaaga aaaagtagag
    35281 gcaatcgtga ttgccggaga cttatatgat aggagtgtcc cagctgttga tgccgtagaa
    35341 ttatacaatg aattgatggt cgattggaat ttaaaggaaa agatcccgat ttttgctata
    35401 tcaggaaatc acgatagcag tacgagactt tcagcgggta cgccatggtt cagttattcg
    35461 aactattttt tgcgaacaca gctagcagaa gcatttgaac cagtcgagtt taaaaacgta
    35521 caatttttct ttcttcctta ctttgaacca attgcagcga gattatattt tgaagatgac
    35581 aatatccgaa cgatcaagca agcggtagcc aaggtgatag ataaaatgca ggagctattt
    35641 gattcagaaa aaaaacaagt attgattagc catttttttg taatgggaag tgtaaaatct
    35701 gattcggaaa cgaaaatcga agtaggcggg ttggattcga tccctggtga actacttaca
    35761 ccgtttgatt atgtagcact aggacatttg cataatcaag ctgctttgag acaggataat
    35821 gctcgctata gcggttcccc attgaaattc tctttatctg agatcaacca aaaaaaaggc
    35881 gtatggatcg ttgacctgac aacaggcctg gagttgaatt ttagggagat cgaacccctg
    35941 catgatattg aagaaataac agcgagtttc aaagaattgc tggatcctgc cttttatcag
    36001 tccgtcaatc gggagaatta catctatatc tatctcactg atagaacgat catcccgaat
    36061 atgatgaacc agcttcggca aatatatcct cgtattttgg ggattgaacg ggcaaacggt
    36121 cgagcagaac aaacaaagcg caaacgaaaa gtactagaaa tgaaagatcc tcaaaaatta
    36181 gcagaagcat tttttagtga aatgacggaa gagcagctaa ctgcaaacca agaaaaatgg
    36241 ctcggagaaa cgttgcaaga aatcatggaa atggaatgat caagaatgaa accgaaaaaa
    36301 ttacaactta aaaattttgg tccttttatc aatgagaccg tagatttttc acgtttgaca
    36361 gaagcaccgc tttttttgat cagcgggaag acaggagctg gcaaaacgac gatttttgat
    36421 gggatcacct tcgcactttt tggtgaaact tccggaagac ttcgctctgg aaaagagatg
    36481 cgttctttgt ttgctacacc agacgaagaa acaagcgtga ctttttcttt tgaacatcag
    36541 cagatgagct atgaaatcga acgaaaacca gaacaagttt tagcaaaacg aaaaggaaac
    36601 ggaacaaaaa aacaggcagc caaagtaacg ttgaccattt ttgatgggaa aggaaaagaa
    36661 ataaaacaaa tcacaaaacg tacagaagca gatcagttga tcagagaact gatgaatctc
    36721 gatgcgaaac agttttctca aatcgtttta ctaccccaag gagaattccg taattttttg
    36781 gtttcatcaa gcagtgataa agagactgtt ttacgtcatt tattcggtac tcaatttttc
    36841 caacagttca atgagcgatt aaaagaaaaa gcaaaacaac agcaacaaaa attggatcat
    36901 ttagagcaag agctgcatct tctgcaaaaa aggtttgttt tagtcaaaga agaggaacca
    36961 gcagcagggt ttgaagccgt tttagcccaa tggacaaacc atcaggcaat actcgaaaaa
    37021 caaatcacgg aagaaaaaga agctctaacg gaacttcagg aaaaacaaaa gcaactagaa
    37081 aattcctact atcgttttga aagcttgaaa aaaagttatg aagaaaaaac gatattaata
    37141 aacaagcaaa aggaattagc tgctcaaaaa gacgaaatag aggaaaagaa acgatggatt
    37201 gggtattatg aatttacgca acgattagtc gaaccaatcc agcgcctaca agaaataaaa
    37261 gttgaaaaaa tagaactgtc acaaaaaagt agtgagcaaa caaagaaact agcgatgact
    37321 acgcagaatt atgaagagtg gcaaaaaaat gaagcaaacc gcgtacgccg acaaacaaaa
    37381 atcaaagaag ctgctcaaaa actgcaaaac aaacagatga tggtgccaat tgtcgaatcc
    37441 ttacgtagta aaaaacaaga cgcagaacaa ttaacaattt cattggataa agaagaattg
    37501 aatgccgaga agctgtatca aaatctcgct atctatcagg aaagaactcg aattcttcaa
    37561 gaaaatttac tggatcagga aaaactgcaa gaagatcgtc ttgcttttgg acagttaaaa
    37621 tatctagatg aaaagcagca agaaatagaa aaacaaaaag caaaagacac tgggtcgctc
    37681 gcacagataa gagaagaact caaaaaagca actgaagaaa taacaaaaaa tagtcagcta
    37741 ttgaaagaac agacagaaca agcagaacag ctgaaaagcc aatgggcaaa gcagcaaatt
    37801 gctcgtcttc agcttttttt gcgtccaggg gagccatgtc ttgtttgtgg atcaacggaa
    37861 caccccatac aacaattaaa acacgaacag ccaaataagg aagatatctt aaaagtagaa
    37921 caacaactgg cagaagctga gtctgctgta gaaagaactc agcaaatcat tgccaaaagt
    37981 gaagcacatg aaaataatct tcaacaacaa ttatcagatt tagaacaaaa aatcatggca
    38041 acgaatatac aattggctga acaagaagaa cacttgcaac aggctttgaa aaaggcattt
    38101 cctgagataa cagaacaaaa aacaaaaaca actcttgtag taatcgaaca atctttagat
    38161 agacgaaaaa gcaagatggc caatgcaaga atagagctgg aagaaataag acaacaagca
    38221 gaaaagctcg aacaacaatt agctgtctac aaacagcagc agacagaaaa aaataatcag
    38281 ctccagcaac tgcaaggaga aatcaaaagt ttagaacaac aattgcaaga tacggatagt
    38341 aactgtttag aagaggagat acgacgactt gaaaaacagt tagaagaaga ctcgaatcaa
    38401 ctagctgttg agcagaaagc aggagtgcaa ttgttgcaag atcttcatac gcttcggaca
    38461 cagcttgatc ttcaacatga acaaatagaa aaaatagatg aaaaaataga aaaggaacaa
    38521 gaaagaatag ctaaaaagtt agaaagccag tcgtattttt caacaattga agaaatctgt
    38581 gcatttgcac aacagcaagc acaatataaa gaaaaccaac gtacagttac ttcttatcat
    38641 gaagagcaat tagtgacagc tgaccggctg cgtcaattag aaaatatcca gctaactgag
    38701 acattcccag atacagaaga gatcagccaa cagttagaag aaataagacg tatgatcgaa
    38761 ataaagcaag aaaagatata cggtttacaa gaacaaaaaa ttagtaatca aaaaatctac
    38821 gatgaatgtc tttcgattta tcacacaagc cagacgaaat tagatgagct tacgcaatta
    38881 cagcagcttt cgcaaacatt caacggagaa aatcctaaga agactagttt ggaacggtat
    38941 gtgcttcagg tttatcttca agaagtgtta caagtagcaa atgatcatct ccaacgttta
    39001 acgaaaaatc gttatcaatt tgagctagcg gataccattg gcagttatcg agggaaaacc
    39061 ggattagaaa tcaatatcta tgatgatcag gcaggtatgt ctcgaagtgc gcataccctg
    39121 tctggcggtg agagttttat tgcagcactt tctttagcat tagctcttgc cgatgtgatc
    39181 cagacacaag ctggtggtgt agcaatcgat gcattgttta ttgatgaagg atttgggtca
    39241 ttagatgaag aagctttgga aatggcaatg gaagctttag aaacgatcga aagtgaagga
    39301 agaatgatcg gcatcattag tcatgtaaga gaattgaaag aacgagtatt acaacaaata
    39361 agaattgata cagaaggtag cggacaaagc aaaatacgct atcaacttgg ataaaaaggg
    39421 agattggtaa ctatgaaatg gagtattcca gaaaaagtca ttgcacgtgg acgtcaatac
    39481 ttgcaagaag gccgagtgtt atcggttgtc cctgaccaag aagaaaacgt gtggcatgca
    39541 gaagtcttgg gcagtgaatt gtatatggtt gatttggatg gtacagcaaa agagatagat
    39601 tactgccaat gtccctattg ggaggaacat aaatattgta agcatacagt tgctgtggaa
    39661 ctttatttac ggtcgaaaaa tctatctcgt atcatgaaaa aagaccagcc aatgctagaa
    39721 ccggtaaaag caacaagtga gggagaaatc attgcaaaag gtttttctcg tctaaaaaca
    39781 caaaacaatc ctcaagcggt gcaacctttg attattgaat accaagtaga aacgattgag
    39841 actaatcagt atcacccaga gctttctatt ttggcacttt acttgcgtat cggttcattg
    39901 gcatcaccga aaaaaacata tatcgtaaaa aacatctatg attttttaca agcttacaca
    39961 gaaaaagaaa cttacactgt taataaacag taccagtttc gtctagataa acgggcgttt
    40021 caaaaagaag atcaaaaaat actcacgcat cttgcaggaa gtgctcagac gcatcaatta
    40081 ttaggtacga acggcttaca agtcaaagga aaactagata aaagatatct gcttctccca
    40141 gtagaacaag cccgcttcct gttagagcag atgaatcaga ctacacgttt gcatctgcag
    40201 ataaatgaac aaaacgatca aggaatacaa ttttccgatc aaaacaaacc tttatcattc
    40261 aaagtggaaa aatttggaga agattatttg ctgcaagcat tgaatgattt tgattttttc
    40321 tttatgcatt atcagtgggg agttatccaa gggacatttt attctctgac gaaatatcaa
    40381 caaacggttt atcaaacttg gaaacaattg attcgccgat tggagaaacc agaagtagtg
    40441 ttcaagaaaa aagaattacc gtctttgttt aaagagatta tccctctgct gtcagagatc
    40501 ggcgacgtag ctattgcccc tgcagtagaa gaggaagtag cagatatccc cgtcgaattt
    40561 attttctttt tccgaaaagc aaaaggaaaa atccaagctc gagtcgattt cgtctatggt
    40621 gaagtcatct attcgactga cccgaagcat gaggtctcga cagaacaggc ttttcaggtg
    40681 ttacgtgaca cagtagaaga gaaacgagta cttgatctat tcagaatgta tcgctaccag
    40741 aaaaatgaaa ctggttatga acgtccattg ccaagcgggg aagaactata tgcctttttc
    40801 cgcacagaac tatctgtctt ccgtcagttt ggtgaagtca gacttggaag aaaattgcga
    40861 gagttgtatt tagatgcgca taaacatcaa ccgaaaatcg aagtaaccga gaccggttca
    40921 tggctcgata ttcgattcga tgtgacaggg atagaggagc aagaaatcga tcatgtcctg
    40981 caaagcttgc tgagaaatga tgctttttat acattagaaa atggtcaggt tctttctttt
    41041 gattcagaag aatttcaaca aacaagccaa gtgttgcaac aattacggga atcgatacgt
    41101 acagaagaag gaacgatcca tgtacctaaa aatcaaggat tgatcattca aaatcaattg
    41161 gaaaaaagca atgctacttt tagtgaatcg ttccaaacga tggtacaaga tctcattcat
    41221 ccagaaagat accaggcaca gcttcccaaa ggattgaatg caacgatgag agattatcaa
    41281 aagcaaggtt ttcgatggtt gaagatgtta ggtcattatc agtttggcgg tattttagcc
    41341 gatgaaatgg gacttgggaa aacactacag acaattgcct ttttactttc ggaaaaagaa
    41401 gaacggaaaa gcttctctgc tttgatcgta gcgcctgcga gcttaattta caactggcag
    41461 gcagaggtga gaaaattcgc cccttcttta agtatccaag taatcaatgg gaataaaaaa
    41521 gaacgtgaag aattactggc taaagacaca gatatccgag tgacttctta tgccagctta
    41581 agacaggatc tagctaatta tcagtcgcaa aaaattgatt atttgattct tgatgaggca
    41641 caaatggtca aaaatagcag tacgaaaact gcacaagcat tgagagaatt agctgtacct
    41701 cagcggtttg cactgagtgg gacaccaatc gagaataatt tagaagaatt gtggtcattg
    41761 tttgcaacga ttatgccagg atttttcccg actaaaacaa aattcaggga acttgccgca
    41821 gaagaaattg cgcaaatgat tcgaccgttt attttacggc gagataaaca aactgtgttg
    41881 aaagacttgc cggaaaaaac agagatgaat ctatattctg cacttacaga agaacaaaaa
    41941 actgtttatt tagcttactt gcgtcaaatg cgtgaagaaa tcacatcaat ggattctgaa
    42001 gcattcaaga aaaaccggat cgggatactt gctggtctga caagactgcg acaaatttgc
    42061 tgcgatcctc gtctgtttat tgaagattac caaggcggtt ccggcaagct cgaacaagta
    42121 aaagatctac tggtagctgc caaagagaac aaacgaagag ttttgctgtt ttctcaattt
    42181 acagggatgc tgactatctt gcaagaagag ttagccgagc tagggatctc gacattttat
    42241 ttaagaggca gtacgaaacc acaagatcgg ttaagtatgg tagacgcatt taatgctggc
    42301 gaaaaagatg tttttctgat ttccttaaag gctgggggaa ctggattgaa tctcacggga
    42361 gcagatacgg tcatcttgta tgacctttgg tggaatcctg ccattgaaga acaagctgct
    42421 ggacgtgcac atcggatcgg acaaaaaaat gtggtagaag tgtggcgtat gatctctgaa
    42481 ggaacgattg aggaacgcat ggattcactc cagcaagaaa aacgcgaact gttccaaaaa
    42541 gtgatccaag gtaatgaaga acagttgacg aaactgactg aagaagacat ccgcatgatt
    42601 ttaagtgtag gagaaatcga ggagtaagag actcgaaaaa gcgttcagta atgggtaaga
    42661 aagcctttct aaagaagcga ttgtttattt gatttaaaaa atagcaagga ggctgggatt
    42721 ccattttatc ccagcttcct tgctatcatt tttttatttc tatttttaaa ggttatacaa
    42781 agattgtctt cattgtctct aacagctgat caaaagaaga aatctcgata tcgcaaattg
    42841 gagtctgttc aattttgcgt tctcgatggt tgaaccaaag cgcttgccag ttagcacttt
    42901 ttgcacctag aacatcattg tcgtaattat cgccaacgta taaggtctct tctggaagca
    42961 tatgaaattc tttctcagcc agctgaaaaa tctctttttc tggtttttgg aaacctgttg
    43021 cttgagaaat gagcatattg cgggaaggaa tccaattgtt caattgaagt tgcttcagtt
    43081 tttttgtctg atggtcagtc ggcccatttg tgataattcc taatgggaca ttcttttcct
    43141 tcaagaaatc gagggttttt ttgacctcct catgcaagca gatattatca agttcttcct
    43201 cgtaaatttt ttgaaataag aggccatctt cttccgtaat atgagggtag tctaaatctt
    43261 ttagtgattg gctgatccga tgtgctcgca tatattctag tgtccaatct cctgccatta
    43321 cttttgggaa gttttcatca ctatgatggc ggaaccggat atatagtgga tgcatatctg
    43381 catctgaaac aagcggaacg actcttttga ccgcgtttcg aaatggctgc tgctgatcat
    43441 acatcgtatc atcaacatca aacacaactg atttaatcaa aattttacac gcctttcggt
    43501 tttcattgat tttttcacaa atccttgact attgtaacag aaaaaattgt ctaaaaaagc
    43561 tgataaataa aggtttttct tactatattt ctatttcctt gacagaataa ggtttttata
    43621 tttcagtttt tttcactaaa aatcgtgaaa cacgtcaata agtttgatga acaaattaat
    43681 cattatggta gaagtcgatt gatttatttt aatgcaatct ctgtggaatt ttcgaaagat
    43741 ttattttttt aatacagtct caatataact attcaaacat aaaataacgt tatttgattt
    43801 atttgtttat agtaaatttt atttagtaat cattgcttta aaatactacg cttatataac
    43861 agagaaaata agcgttataa taaagaacag tattatttaa aat
//
LOCUS       DAZKLA010000010        40931 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000010 DAZKLA010000000
VERSION     DAZKLA010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.010"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..1189)
                     /locus_tag="ACG85N_000430"
     CDS             complement(38..1189)
                     /locus_tag="ACG85N_000430"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFQ0223229.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="ACG85N_000431"
     CDS             complement(1202..1639)
                     /locus_tag="ACG85N_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0223230.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGGVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="ACG85N_000432"
     CDS             complement(1654..3420)
                     /locus_tag="ACG85N_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFQ0223231.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="ACG85N_000433"
     CDS             3684..4361
                     /locus_tag="ACG85N_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0223232.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="ACG85N_000434"
     CDS             4364..5491
                     /locus_tag="ACG85N_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0223233.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="ACG85N_000435"
     CDS             complement(5691..6131)
                     /locus_tag="ACG85N_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0223234.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="ACG85N_000436"
     CDS             complement(6142..8175)
                     /locus_tag="ACG85N_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFQ0223235.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="ACG85N_000437"
     CDS             complement(8177..9880)
                     /locus_tag="ACG85N_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0223236.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACG85N_000438"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACG85N_000438"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFQ0223237.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="ACG85N_000439"
     CDS             complement(10963..11988)
                     /locus_tag="ACG85N_000439"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0223238.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKTVLPK"
     gene            complement(12197..13066)
                     /locus_tag="ACG85N_000440"
     CDS             complement(12197..13066)
                     /locus_tag="ACG85N_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0223239.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="ACG85N_000441"
     CDS             complement(13525..15417)
                     /locus_tag="ACG85N_000441"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0223240.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="ACG85N_000442"
     CDS             complement(15414..17141)
                     /locus_tag="ACG85N_000442"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0223241.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="ACG85N_000443"
     CDS             complement(17157..17807)
                     /locus_tag="ACG85N_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFQ0223242.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="ACG85N_000444"
     CDS             18011..18307
                     /locus_tag="ACG85N_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0223243.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="ACG85N_000445"
     CDS             18479..19099
                     /locus_tag="ACG85N_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0223244.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="ACG85N_000446"
     CDS             complement(19191..20357)
                     /locus_tag="ACG85N_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0223245.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="ACG85N_000447"
     CDS             complement(20369..20722)
                     /locus_tag="ACG85N_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0223246.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="ACG85N_000448"
     CDS             20850..21872
                     /locus_tag="ACG85N_000448"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFQ0223247.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="ACG85N_000449"
     CDS             21891..22052
                     /locus_tag="ACG85N_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223248.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="ACG85N_000450"
     CDS             complement(22311..22745)
                     /locus_tag="ACG85N_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223249.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="ACG85N_000451"
     CDS             complement(22855..24714)
                     /locus_tag="ACG85N_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0223250.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            24715..24855
                     /locus_tag="ACG85N_000452"
     CDS             24715..24855
                     /locus_tag="ACG85N_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0223251.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(24832..25992)
                     /locus_tag="ACG85N_000453"
     CDS             complement(24832..25992)
                     /locus_tag="ACG85N_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0223252.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACG85N_000454"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACG85N_000454"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFQ0223253.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="ACG85N_000455"
     CDS             complement(27739..28437)
                     /locus_tag="ACG85N_000455"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0223254.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="ACG85N_000456"
     CDS             complement(28394..30055)
                     /locus_tag="ACG85N_000456"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFQ0223255.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="ACG85N_000457"
     CDS             30273..31673
                     /locus_tag="ACG85N_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFQ0223256.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="ACG85N_000458"
     CDS             complement(31925..33004)
                     /locus_tag="ACG85N_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0223257.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="ACG85N_000459"
     CDS             complement(33090..34049)
                     /locus_tag="ACG85N_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0223258.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="ACG85N_000460"
     CDS             complement(34061..35572)
                     /locus_tag="ACG85N_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0223259.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="ACG85N_000461"
     CDS             complement(35594..36157)
                     /locus_tag="ACG85N_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0223260.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="ACG85N_000462"
     CDS             complement(36163..37026)
                     /locus_tag="ACG85N_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0223261.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="ACG85N_000463"
     CDS             complement(37040..37936)
                     /locus_tag="ACG85N_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0223262.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="ACG85N_000464"
     CDS             complement(38111..39400)
                     /locus_tag="ACG85N_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0223263.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="ACG85N_000465"
     CDS             complement(39372..40889)
                     /locus_tag="ACG85N_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFQ0223264.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT        11793 a         8582 c         7090 g        13466 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgcc ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgt
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt t
//
LOCUS       DAZKLA010000011        38245 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000011 DAZKLA010000000
VERSION     DAZKLA010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.011"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..347
                     /locus_tag="ACG85N_000466"
     CDS             72..347
                     /locus_tag="ACG85N_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFQ0223265.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            353..1120
                     /locus_tag="ACG85N_000467"
     CDS             353..1120
                     /locus_tag="ACG85N_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFQ0223266.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            1288..3117
                     /gene="typA"
                     /locus_tag="ACG85N_000468"
     CDS             1288..3117
                     /gene="typA"
                     /locus_tag="ACG85N_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFQ0223267.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            3378..5150
                     /gene="recQ"
                     /locus_tag="ACG85N_000469"
     CDS             3378..5150
                     /gene="recQ"
                     /locus_tag="ACG85N_000469"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFQ0223268.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            5264..5566
                     /locus_tag="ACG85N_000470"
     CDS             5264..5566
                     /locus_tag="ACG85N_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFQ0223269.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            5569..6732
                     /locus_tag="ACG85N_000471"
     CDS             5569..6732
                     /locus_tag="ACG85N_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0223270.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            6758..10186
                     /locus_tag="ACG85N_000472"
     CDS             6758..10186
                     /locus_tag="ACG85N_000472"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFQ0223271.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            10207..11331
                     /locus_tag="ACG85N_000473"
     CDS             10207..11331
                     /locus_tag="ACG85N_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFQ0223272.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            11312..11758
                     /locus_tag="ACG85N_000474"
     CDS             11312..11758
                     /locus_tag="ACG85N_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0223273.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            11742..12080
                     /locus_tag="ACG85N_000475"
     CDS             11742..12080
                     /locus_tag="ACG85N_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFQ0223274.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            12199..12759
                     /gene="rsmD"
                     /locus_tag="ACG85N_000476"
     CDS             12199..12759
                     /gene="rsmD"
                     /locus_tag="ACG85N_000476"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFQ0223275.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            12756..13247
                     /gene="coaD"
                     /locus_tag="ACG85N_000477"
     CDS             12756..13247
                     /gene="coaD"
                     /locus_tag="ACG85N_000477"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFQ0223276.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            13237..14280
                     /locus_tag="ACG85N_000478"
     CDS             13237..14280
                     /locus_tag="ACG85N_000478"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFQ0223277.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            14439..15167
                     /locus_tag="ACG85N_000479"
     CDS             14439..15167
                     /locus_tag="ACG85N_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFQ0223278.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            15283..15528
                     /locus_tag="ACG85N_000480"
     CDS             15283..15528
                     /locus_tag="ACG85N_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0223279.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            15525..15743
                     /locus_tag="ACG85N_000481"
     CDS             15525..15743
                     /locus_tag="ACG85N_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFQ0223280.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            15879..16418
                     /locus_tag="ACG85N_000482"
     CDS             15879..16418
                     /locus_tag="ACG85N_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFQ0223281.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            16411..18684
                     /locus_tag="ACG85N_000483"
     CDS             16411..18684
                     /locus_tag="ACG85N_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFQ0223282.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            18722..19768
                     /gene="holA"
                     /locus_tag="ACG85N_000484"
     CDS             18722..19768
                     /gene="holA"
                     /locus_tag="ACG85N_000484"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFQ0223283.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(19857..20111)
                     /gene="rpsT"
                     /locus_tag="ACG85N_000485"
     CDS             complement(19857..20111)
                     /gene="rpsT"
                     /locus_tag="ACG85N_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFQ0223284.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(20511..20780)
                     /locus_tag="ACG85N_000486"
     CDS             complement(20511..20780)
                     /locus_tag="ACG85N_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223285.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(21042..21170)
                     /locus_tag="ACG85N_000487"
     CDS             complement(21042..21170)
                     /locus_tag="ACG85N_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223286.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(21250..21705)
                     /locus_tag="ACG85N_000488"
     CDS             complement(21250..21705)
                     /locus_tag="ACG85N_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFQ0223287.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(21843..23771)
                     /locus_tag="ACG85N_000489"
     CDS             complement(21843..23771)
                     /locus_tag="ACG85N_000489"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0223288.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(23968..24801)
                     /locus_tag="ACG85N_000490"
     CDS             complement(23968..24801)
                     /locus_tag="ACG85N_000490"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFQ0223289.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(24993..25697)
                     /locus_tag="ACG85N_000491"
     CDS             complement(24993..25697)
                     /locus_tag="ACG85N_000491"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0223290.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(25720..26346)
                     /locus_tag="ACG85N_000492"
     CDS             complement(25720..26346)
                     /locus_tag="ACG85N_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0223291.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            26672..29047
                     /locus_tag="ACG85N_000493"
     CDS             26672..29047
                     /locus_tag="ACG85N_000493"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFQ0223292.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(29071..29562)
                     /locus_tag="ACG85N_000494"
     CDS             complement(29071..29562)
                     /locus_tag="ACG85N_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFQ0223293.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            29749..30669
                     /locus_tag="ACG85N_000495"
     CDS             29749..30669
                     /locus_tag="ACG85N_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0223294.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            30823..31167
                     /locus_tag="ACG85N_000496"
     CDS             30823..31167
                     /locus_tag="ACG85N_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFQ0223295.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(31357..31857)
                     /locus_tag="ACG85N_000497"
     CDS             complement(31357..31857)
                     /locus_tag="ACG85N_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0223296.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            32385..32468
                     /locus_tag="ACG85N_000498"
     tRNA            32385..32468
                     /locus_tag="ACG85N_000498"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32419..32421,aa:Leu,seq:cag)
     gene            complement(32792..33430)
                     /locus_tag="ACG85N_000499"
     CDS             complement(32792..33430)
                     /locus_tag="ACG85N_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0223297.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            33765..34199
                     /locus_tag="ACG85N_000500"
     CDS             33765..34199
                     /locus_tag="ACG85N_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0223298.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            34222..34542
                     /locus_tag="ACG85N_000501"
     CDS             34222..34542
                     /locus_tag="ACG85N_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223299.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            34683..34874
                     /locus_tag="ACG85N_000502"
     CDS             34683..34874
                     /locus_tag="ACG85N_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223300.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(34945..35202)
                     /locus_tag="ACG85N_000503"
     CDS             complement(34945..35202)
                     /locus_tag="ACG85N_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFQ0223301.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            35360..35623
                     /locus_tag="ACG85N_000504"
     CDS             35360..35623
                     /locus_tag="ACG85N_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223302.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            35647..35835
                     /locus_tag="ACG85N_000505"
     CDS             35647..35835
                     /locus_tag="ACG85N_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223303.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(35918..36103)
                     /locus_tag="ACG85N_000506"
     CDS             complement(35918..36103)
                     /locus_tag="ACG85N_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223304.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            36318..36689
                     /locus_tag="ACG85N_000507"
     CDS             36318..36689
                     /locus_tag="ACG85N_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0223305.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(36839..36911)
                     /locus_tag="ACG85N_000508"
     tRNA            complement(36839..36911)
                     /locus_tag="ACG85N_000508"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36876..36878),aa:Gln,seq:ctg)
     gene            complement(36957..37385)
                     /locus_tag="ACG85N_000509"
     CDS             complement(36957..37385)
                     /locus_tag="ACG85N_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223306.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(37445..38203)
                     /locus_tag="ACG85N_000510"
     CDS             complement(37445..38203)
                     /locus_tag="ACG85N_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0223307.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT        12735 a         6553 c         7758 g        11199 t
ORIGIN      
        1 atgacaactt cttggtttat taccacttcc tatgctacac tgaagaggaa tatgaaatag
       61 gagagttgaa gatgcaagaa tatcagtatc cacttgattt agattggaca acagacgaaa
      121 tggtcattgt aatgaagatg tgggaagatc tggaacgtgc gaatgaaaaa gggataaaca
      181 atgaagcttt tttgaagact tatcagcagt ttaaaactgt tgtcaaatcc attggtgagg
      241 aaaaacgatt gggacgggaa tttgaaaaag cttccggtta ttctttatat cgtacagtgc
      301 agcaagcaaa aaaaaatcca aacaaaactt tgagaatgaa cgggtgagat aaatggaaca
      361 gatgattgat catatcaagt cctggcttga agaagctggt gagaaaatca aatcttctca
      421 acaaacatta acaatctcgc aaaaatcaaa tcgaacggat ctagtaacaa atgtcgatcg
      481 aaacatccag aattttctaa tcgaaaaaat ccattccttt gatccagagg cgaaaatatt
      541 ggcagaagaa gaagggtgta atcaattatc ggatctcgat ggaagagtct ttatcatcga
      601 tcctattgat ggcaccttga attttgtgat tgaaggggaa aatttttgta ttatgctcgc
      661 tgtttatgaa tccggcgttg gaaagcttgg tttcatttat gacgtgatga gaaatgaact
      721 tttttggggt ggaaaaggaa taggcgtgta taaaaatgat gagccattgc ctagaccagc
      781 aaataaatcc ttatcggaag gcttacttgg tgtgaatagt tatcttttcg gtcataataa
      841 atatggaatc cgagaaattg cggaaagcag catggggaca cgtatgtacg gatgcgctgg
      901 tcttgaacta gtggcaatct tgaaaggaaa ccacattgct tatatttcaa accttagccc
      961 ttgggattat gcagcgggaa atgtcttatt ggaagagttt ggtatggtaa caagcggtct
     1021 ttttggtact cctttgacat ttgaaaaaag ggaatactat ttagcaggga cttcttgtgc
     1081 atatgcagaa atcgcagacc ttttgaaaaa agaaggctga aaaaaaggta gaaatcattc
     1141 acaaaaatga taaaaacgag caaaagatta attttctcga aaaaatgaaa acattttact
     1201 atatttcatg aaaaaagtct gctataatta gcagtcgagt aaaatttgcc ctgtcataaa
     1261 gaaccaataa aaaaggagtt agttacattg aattatagaa acgatatccg taacgtcgca
     1321 attattgccc acgttgacca cggaaaaaca acattagttg atgaactatt aaaacaatca
     1381 gatacattag acgcacatac gcaattgcaa gaacgtgcga tggactcaaa tgcgctagaa
     1441 aaagaacgtg gtatcacgat tttagccaaa aacacagctg tagactataa aggtattcgc
     1501 gtaaacatca tggatactcc aggacacgcg gacttcggtg gcgaagtaga acggatcatg
     1561 aaaatggttg acggcgttgt gttggtcgta gatgcgtatg aaggaacaat gcctcaaaca
     1621 cgttttgtat tgaaaaaagc actggaacaa catatcacac caatcgttgt cgtaaacaag
     1681 attgacaaac cttctgctcg tcctgagcat gtcgtagatg aagttcttga actattcatc
     1741 gaattaggag cagatgatga tcagttagac tttcctgtga tttatgcatc tgcactaaat
     1801 ggtacgtcaa gcttgtcaga tgatccagca gatcaagaac caaccatggc acctattttt
     1861 gatacaatta ttgaaaaaat ccctgctcct gtcgataaca gcgacgaacc attacaattc
     1921 caagtttcat tacttgacta caacgattat gtaggtcgta tcggaatcgg acgtgtattt
     1981 agaggaacaa tcaaagtagg agaccaagta gcattaatta aattagacgg aacagttaag
     2041 aaattccgtg taacgaaatt atttggtttc tttggactaa aacgtctaga gattcaagaa
     2101 gcaaaagctg gagacttgat tgctgtctca ggtatggaag atatctttgt cggtgaaaca
     2161 gttactcctg tggatcatca agatgcgtta cctatccttc acattgacga accaacattg
     2221 caaatgactt tcttagttaa taattcacca tttgccggtc gagaaggtaa attcgtaacc
     2281 gctcgtaaga ttgaagaacg tttgatggca gaacttcaaa cagatgtatc cttacgagtt
     2341 gaaccaacaa actctccaga tgcatggaca gtttctggtc gtggagaatt gcatttgtct
     2401 atcttgattg aaaacatgcg ccgtgaggga tacgaattgc aagtatctcg tccagaagtt
     2461 attgaaaaag aaatcgacgg tgtaaaatgc gaaccgtttg aacgcgtgca aatcgataca
     2521 ccagaagaat atatgggtag tgttatcgag tccttgagct tacgtaaagg tgaaatgcaa
     2581 gatatggtcc atacaggaaa cggacaaatc cgtttgacat tcttaacacc tgcccgcggt
     2641 ttgattggtt attcaactga attcctttca atgacacgtg gttacggaat catgaaccat
     2701 acttttgacc aatatctgcc aatgcttcct ggtcaaattg gcggacgtca ccaaggggcg
     2761 cttgtttcga tcgatacagg aaaagcaacg acttattcta tcatgagtat cgaagaacgc
     2821 ggtacagtct tcgtagaacc aggtacagaa gtatatgaag gaatgattat cggggaaaac
     2881 agccgtgata atgatttgac tgtcaacatc acgaaagcaa aacaaatgac aaacgtacgg
     2941 tctgctacta aagaccaaac atctgtcatc aaaaaaccaa aacaattgac gttggaagaa
     3001 tctttggaat tcttgaatga tgatgaatat tgcgaagtaa caccagaaag cattcgcttg
     3061 agaaaacaaa ttttagagaa aaatgctcgt gaaaaagcat ctaaaaagaa aaaataatct
     3121 gtaaaaggat gaagagagaa accagcttgt tataaaaaat agctggtttc tttttttgtc
     3181 caactctttt ctgtggagaa gtcatctttg tcaatggaca taaattcaga aaatacgccg
     3241 aaagacgttt aaaagacttt taaaaaactt atttctagag agtttttgtt tcataataag
     3301 ataatgtttc acgaaaggag atcaacttgt ccttcgaaga aaatcgaagt aaaatggcaa
     3361 gagaaaggag cgggaagatg aacgacttat taaaacaata ctttggctat gatgagttta
     3421 gacctggtca aaaagaaatt atccagaaag tcattgatca ggaaaatgta ttaggaatca
     3481 tgcctaccgg aagtgggaaa tcaatctgtt accagcttcc agctttacta ttagatgggc
     3541 ttactgtggt tgtgtcccct ttaatctctt tgatgaagga tcaggtggac gcggcgaatc
     3601 aattaggtat acccgccact tttatcaata gctcgttaga tggttacgaa acagctagac
     3661 gcttccaaga aatcgataga cagcagtacc gtttacttta tatcgctcct gaacgtttta
     3721 tcatgccgga ttttatccaa gcaatgaagc gatggaacgt ccgtatgatc gctattgacg
     3781 aggcacattg tatctctcag tgggggcatg atttccgccc aagttattta cagatggcga
     3841 accagctaga tcaactgcca aatcgtcctg taattgttgc tttgacagct acagcgactg
     3901 tgcaagtagc tgctgacata aaaagacttt taaaaattcc agaaaacaac catattcaga
     3961 ctggttttga acgagaaaat ctacgatttc aagtaattaa agatcaaaaa aaagaacaat
     4021 atctaatcga atatttaaag atcaacaaaa atcaatctgg tatcatttat gcagcaacga
     4081 gaaaagaagt agaccgtctc tatcatttgc ttaaaaaatt cgatttttcc gtgggtagat
     4141 atcatggtgg tctaaatgag aatgagcgaa cagaaatgca agaagccttt ttatacgatc
     4201 gcttgcagct gattgtagcg acaaatgcat ttggtatggg tataaataaa agcaatgtcc
     4261 gttttgttat ccattatcaa attcctggct ctctagaagc atactatcag gaagctggtc
     4321 gggcgggaag agatggtctt tccagtgaag ccatcctgct tttctctcct caagatatcc
     4381 aagtccaaaa gttttttatc cagcagtccc aaagagaaga agggcaaaag caaaaagaat
     4441 atgaaaaatt aaaagcgatg acagaatacg tgtatattga gtcctgtctt cagcaataca
     4501 tcctaaacta ttttggcgag acaagcagtc cgtgcaaccg ttgtggtaac tgtttagatg
     4561 acagagaaat agtagaagta acgacagaag cacagatggt tctttcttgc ttaaaacgta
     4621 tgggtgagaa ctatggtaaa caaatgctga tgaaagtctt agcaggatcg aaagaacaaa
     4681 agctgcaagc tttaggtttt ggacatttat ctacctatgg actgatgaaa aaccaatcgc
     4741 aaaaagaaac gatgcagtta attgaatatt tgatatcgaa tggctatctt ctgaccatca
     4801 atggtgagta tcctgttttg aaagtaacag aaagagggat acaagtattg aaaggacaag
     4861 aatctgttta tcgaaaggaa cccaagaaag tccagcagct atcggatgaa gaaaccgata
     4921 cactatttga agtgctgcgg gaactgcgaa cggatctagc atcagaagca ggcgttcccc
     4981 cgtatgtcgt attttcagat tccacattga aagaaatgag ccgaatacgt ccatcaagca
     5041 gattggagat gcttcagata aaaggagttg gccaaagcaa gcttgataaa tatggcgaag
     5101 cctttttatc tcggataaaa aatgcggact ctaacgttct ggataagtag aaagaacgaa
     5161 tacgtaaaaa tgaataaaaa caactggtta aatctgtttt ttttgctcct tatgaaaatc
     5221 aaatatgtta taattaagga attagaatgg gaggtaagca cagatggatt ctttgtcgaa
     5281 aaaacttgcg atccaagtat taaatgatga agcagaacgg atcaaacgat taatccgaaa
     5341 tcaaaacaac agtctctgta tctcacaatg caaagccttc gaagaagtgg tagatacaca
     5401 gatgtacggc ttttcccggc aagtcggttt tgctgtccgt ataggagtaa tcggaaagaa
     5461 cgagggccag ctgatgttaa gtgagctaga acgagaattg aatcagttat atagtgaggt
     5521 ttaccaagaa atttatgata aaaaagaagt tggcaaggag gaatagactt gcctaagaaa
     5581 gtgaaaaaaa ggcatctatt agactatagc atcttgattc cctatttaat tttatgtgta
     5641 ttgggattga tcatggtata tagttctacg tcgtatctat tattagaaaa cggacaaaat
     5701 ccgtccgcat cagtcattaa ccagagtatt ttttgggtgc ttagcttgat tgctatcgca
     5761 ctactttata aaatgaaaac agatgttttg aaaaaccagc gtttgatcat ggcagccatc
     5821 gcagttttga cgattttgct gctgattgtt gtgttttttg gtaaggagat caatggggcg
     5881 aaaggttggc tgcaaattgc aggattctcg atccaacctg ccgaatacct aaaaatcatt
     5941 tctatctggt acctgtctct gactttatct aaaagacaaa acagtgtaca aaaagatttt
     6001 cttgggactg tgaaacgtcc acttgcaatg gttatcggac ttactgcatt agtcgctatt
     6061 cttcccgatt ttggtaatgc ggcagttatc tttttgatta ttttggttct gcttttagcc
     6121 agcggagtca attatgtgta tactttgatt gtaggagtag gaggcttttg tctaagtact
     6181 tttacgatct ggctgatcaa tatcacaaac ggaaagatct tacccggacg tttacagtat
     6241 atctataatc gatttgctat ttaccaaaat cctttctcag acgaattaaa taaagggcat
     6301 caactagtca acggctacta tgccatgttc aacggaggac tatttggcag gggattaggt
     6361 aacagtatcc agaaaaaagg atttttacaa gaagcacaaa ccgattttat ttatgcaatc
     6421 gtagtggaag aactgggagt gattatgggg attttgatat tagcgttgct attctttatg
     6481 attgtgcgca ttgtccttgt aggtatccgt tcaaaagatc cgttcaattc gcttttgtgc
     6541 attgggatcg gcgcaatgtt tttgatccaa gtatttgtca atcttggagg aattactggc
     6601 gttattcctt tgacagggat cacgttccca tttttaagtc aaggaggctc cagtttgctg
     6661 atgctttcga tttgtgtcgg ctttgtcctc aacatcagtg cggatgaaaa acgcaagagc
     6721 ttaggtttat aataaaaaaa gatgtaggag tggttcgatg aaaaaagttt tagttgctaa
     6781 caggggtgaa atcgctgtac gtgtatttcg tgcttgtaca gaattaggaa ttaaaactgt
     6841 tggaatctat gcagaagaag atgaatattc cgtacaccgt ttcaaagcgg atgaagcata
     6901 tttagtagga caaggaaaaa aaccgattga tgcgtatcta gatattgaag ggatcatttc
     6961 tatcgcgaaa gaatgcggag ctgacgctat tcatccgggt tacggtcttt tgtcggaaaa
     7021 cttgaatttt gcgcaacggt gcgaagaaga aggcatcatt tttgttggac ccaaactgca
     7081 tcatttagat atttttggag ataaaatcaa agcaaaagct gcagctatcg aagcggggat
     7141 tgcttcgatt ccaggcacag atggaccaat cgcttctata gacgatgcct tggagtttgc
     7201 gaaacaatac ggttatccga ttatgatcaa ggcagctcta ggcggcggcg gacgcggcat
     7261 gcgtgtagct cacgatgaaa agagtgcaag agagggctac gaacgtgcga aaagcgaagc
     7321 aaaagcagcc tttggtagtg atgaagtgta tgttgaaaaa tacatcgcta atcctaagca
     7381 tatcgaagta cagattctcg gggatacgca tggaaacgtc atccatcttt ttgaaagaga
     7441 ttgttctgtt cagcgccgtc accaaaaagt agtagaagta gcgccttgtg tctctatgaa
     7501 tgaacagcaa cgtcaaaaaa tctgccaggc agctgtccaa ttgatgaaat atgtcgggta
     7561 tgtcaacgca ggtacagtag aatttcttgt tgaaggagat gatttttact ttatcgaagt
     7621 caaccctcgc gtccaagtgg aacatacgat cacagaaatg attacagata ttgatatcgt
     7681 aacaacgcaa ctgttgattg ctcaaggttt ggatctgcat aaagaaatcg gattgcctca
     7741 gcaagaagga attaaattaa atggttcagc cattcaatgc cgaatcacta cagaagaccc
     7801 gttgaataac tttttaccag acacagggaa aatcgatacg tatcgttcac ctggtggttt
     7861 tggcgtacgt ctggatgtcg gaaatgctta cgcgggctat gtagtgacac cgtattttga
     7921 ttcattgcta gtaaaagtat gtacgcatgg tgctaccttt gaaacagcca ttcagaaaat
     7981 ggaacgttgc ttgaaagaat ttagaatccg tggagtgaaa acaaacattc catttatgct
     8041 caatgtcatc acacatcctg aattccaatc aggaaatgca aaaacaacct tcattgatag
     8101 tacagcaaca ttatttgaat ttcctcgact acgagaccgc ggaaacaaaa cgatgaaata
     8161 tatcggtgaa attactgtga atggttttcc aggtatcgaa agtggcgaaa aaccatttta
     8221 tgaagaacca cgtatgccta aagatctaat cacaagaact gattacgtaa cggctaagaa
     8281 tgtattagat gcgaagggtg ccgatgcttt ggtcgaatgg atcaaaggac aggaaaattt
     8341 gttgttgacg gatacgacat ttagagacgc ccatcaaagt ctgctagcaa ctcgtgttcg
     8401 gacaaaggat ttcaagcaga ttgcacgttt gactggagaa ggtcttcctg aactcttttc
     8461 aagtgagatg tgggggggag caacatttga tgtggcttac cgctttttga atgaagatcc
     8521 ttggcaacgg ttgagaaaaa ttcgttcttt aatgcctaat acgttgctgc aaatgctttt
     8581 cagaggttca aatgccgttg ggtattctaa ttatcctgac aatgtattgg tggaatttgt
     8641 caaagaagca gcagcgcaag gaatcgatgt atttcgaatc tttgatagtt tgaactggac
     8701 tccacaaatg gaaaaaagta tccaagctgt acgggatact ggtaaaatcg cagaggcagc
     8761 tatttgctat actggcgata tcaacgatcc aagtcgggca aaatacaatg ttcagtatta
     8821 taaagacatg gcaaaggaac tagaacaact gggtgcgcat atcattgcaa tcaaagatat
     8881 ggctgggctg cttaaaccgc aggcagctta tcgtttgatt agtgaattga aagaaacgac
     8941 agatctgcca atccatctgc atactcatga taccagcggg aatggaatca tcacttattc
     9001 tgctgcttca aaagcaggag tagacatcgt tgacgtggca atgagtgcaa tgagtgggaa
     9061 tactagtcag cctagcatga gcagcttgta ttatgcactg gtcaatggtc cacggctgcc
     9121 agaaatcacg attgaaaatg cacagaaatt aaaccattat tgggaagatg tgcgcatgta
     9181 ttacaaaccg tttgaaaacg gcttgaatgc tcctgaaaca gaagtatata tgcatgaaat
     9241 gccaggagga cagtattcga atctacagca acaggcaaaa gctgtcggat taggtcatcg
     9301 ctgggatgaa atcaaacaaa tgtatcatac tgtcaacttg atgtttggcg atatcgttaa
     9361 agtaactcca tcatctaaag tagttggaga tatggcacta tttatggtcc aaaatgatct
     9421 tacagaagaa gatatttatg aaaaaggcga aacactaagt ttccctgaat ctgttgtgac
     9481 tttcttccaa ggagaactag ggcaacccgt tggcggattt cctaaaaaat tgcaaaagat
     9541 tattcttaaa ggcagacctg cattgaatga acgtccaggt cttttggctg aatcagtcga
     9601 cttcaacgaa gtaaaaaaag aattggcaga aaaaattggc tatgaaccaa aacaagacga
     9661 agtattaagc tatttgatgt atccgcaagt ctttttggac tatcaaaaag catataatca
     9721 atttggcgat gttacattac tggacacacc aacatttttc caaggtatac gtttgggtga
     9781 aacaatcaat gtacaaatcg aacgcggaaa aacgcttatc attcgtttag atgaaatcgg
     9841 agaacccgat atcgaaggga atcgcgtact gttcttcaat ttgaatggac aaagacgtga
     9901 aatcactatc aaagataatt cgatcatcag tacggtccaa acccgattga aagctgaacc
     9961 gacaaaccga gaacaaattg gagcaacgat gtctggttct gttttggatg tattagtgaa
    10021 aaaaggcgat aacgttaaaa aaggtgatac gttgatgatt actgaagcga tgaaaatgga
    10081 aacggccatc gaagcacgtt ttgacggaga agtcgcacat gtatatgtgt cttcaggaga
    10141 cactatttcg tctggtgacc ttttgattga agtgactgag aaataataag cgaaaggtgg
    10201 ggaaacatga aaagactggg tttgttttgg ctgattctgc tattagttat tgtggcattc
    10261 tacatggaac cagtcttgtt tccgccaaaa cttgaagtga aggaaaactc tgatcaacaa
    10321 atatcaaacc aaatgacaac gtggaaatac caggagctta aagcaactgg tttttctact
    10381 tatatcggaa aaccgatcga tcagctggaa aaggattttg gccaagccta tgatcgatta
    10441 actagcggat ttggttttga aacaagatat tatgaaaata aagctagtca tctcacattt
    10501 gaagcgaaca tccaagacag cagagtcaca actgttaaag tactgcaaac agggcctgct
    10561 gatatcgcgc cgttttattt agggatgacg atgcaagatc ttacgaaaat cactactatc
    10621 tatactaatt tcacttttga gtaccatgat acagaagttg gaatcgaact aatggaagag
    10681 gatatgaatt atcgtccgct aattgctttt gataacgaca cttttgctat tttatttttt
    10741 gatcaaacaa caaacggcct attttcagtt gtgtatttag ataaagatag tcttttgaaa
    10801 ctattgccat atcaagtgtt tggtgaaggc cttccacatt atcaagcaga taaatcggct
    10861 gattgggaat caatcaatcg cgttaaagaa cgaaaaagta cgactttatt aaatatgctt
    10921 agaagagaag acgaacttcc catatacaat caaacacttg cctttcaaaa ccacagtcag
    10981 ctgctactac atgatttttt gacaaggcct gaggaaatat tgtcagagga acgtatggct
    11041 gattggcaaa aatcgctagg cagtgctcaa acgacagtga aattctcact gacgaatgat
    11101 gaattggaga agttaatcag taaacaaaaa ttagaaaata ctagtgggct atttattcat
    11161 ccggcgattg acgctacttt ttcgttttta tattggtaca gcgatcctta caaccatgac
    11221 cgtttcatgg acccgggaac ggattctgta ggcattgctt tttctaaaga aaatatgtta
    11281 gtcttattgc aagaagaaaa cggagaaagt agtgatagca gtgatcgtta acgaagagct
    11341 gtttcaactg gaggatcaat gcctccaagt cgctgaaatg ataaaaaaga gtaaaacgat
    11401 ggcttcttat cttgaacata aacagtcaat ggaagaatct gcaaaagttt gccagttgaa
    11461 agaagcgttc ttgtctaaga aagaagcatt tgaaaatgta gcggcatatg gtgagtatgc
    11521 acctgattat cgtgaaaaaa aacgagctgt tcgaaaagca aaaagacagc ttgatatgga
    11581 tgagcatgtt gcagctttcc gatatagtga aacacagctg caaagtctgt tggatcaaat
    11641 tgtccaagta cttgccgaag aagtttctaa tgaaatcaaa gtagatgcag gtagcccgtt
    11701 ttttgaaacg aaaaaacact ttggatgcgg aggtaattgt catgcaatta gacaatagtg
    11761 aatttgaaat aacaaaacga cgcgggctga tcgtatgggt ctacagctta aaacaattaa
    11821 agaatctgaa aaagttcggc ttgttgcatt atgtctcaag aagaatgaaa tatgtcgtgc
    11881 tttacttaaa tgaagagaca ttggaagaaa cagaagaacg aatccgtaaa ttacattttg
    11941 ttcgaaacgt agaacgttct taccgaccgg atatcgaaat gaattttgcc gaaaaaatag
    12001 gaaacaaaac agtattaaaa gacgaaggtt tcgaaattga agagctaaat acagaaatcc
    12061 gattagctga acatctttaa aaatctctta tgagcagttt ttaaataaaa aataacttgg
    12121 ataacgatcg atttttgatc attgtccaag tatttttttg gtatactagt accagtacca
    12181 tttctaagga gtgagtagat gcggataata tcaggaaatt atggcgggcg ccgtttaaaa
    12241 agtttagcag gggcgaacac cagaccaact acagataaag taaaagaatc catattcaat
    12301 atgattggac catattttga tggagagaca gtattggatc tgtttgcagg aagcggtggt
    12361 ttagctatcg aagcagtatc tagaggctgc agtcatgcgg tatgcgtaga caaaaattac
    12421 caagcgctaa aaatcatcaa agaaaacatc gaaattacga aagaaccaga gaaatttacc
    12481 acaataaaac ttgatgcgga caaagcaatc gaacggctag cagcggagaa acaacaattc
    12541 gactacttgt ttcttgatcc gccatatgca aaacaaaaaa taatcgatca aatccataaa
    12601 atggaagaac tgggactttt ttctaaaacc gcagttatcg tatgcgaaac ggataaaaca
    12661 gtcgaattgc cagaaactgt ggctggattc catcaaataa gaaaacagat ttatggcata
    12721 acggccgtaa ctatctatag aaaagaggga aatcaatgaa acgtgcatta ttcccaggaa
    12781 gttttgatcc ttttacaaaa ggtcacttag atacggtgga acgtgcggct aaattattcg
    12841 atgaggtagt aattggtgtt tttatcaata cttcaaagaa aagtttgttt cccccagaag
    12901 aaagaatgac attgatcaca aaagctgttt ctcatcttcc taatgtaaaa gtcatgcatc
    12961 aagaaaatca attgacggta gaaacagcaa aagagattgg tgcagatgcg ttgatcagag
    13021 gcatacgaag tatcaaagac tttgaatatg aacgagagat tgctcagatg aatcatcatc
    13081 tatatgatga attggaaact gtcttcttat tagcaaaacc agaatatagt cacgtgagtt
    13141 ccagtatttt aaaagaagta ctgcactttg gcggagatgt gagcagctac ttaccgccag
    13201 tgatcaacga agctttagct ggaaagcgtg aatcaaatga aagctaaaca atggttgaaa
    13261 acagtactga ttatcatatt tggaatgttg atcattggat ttgtagtagt tcccttgccg
    13321 tattatgtag aatcaccggg agccacgatc gatctgaaag aactaattac ggtcaatgat
    13381 aaggaagata aaggttctgg gtctttctct cttacctctg ttggtatcag gcaagctaca
    13441 ggtttcttag ctttaaaagc taaaatgacc ccttttgaag aattgatcag tgaagaagaa
    13501 ttgactggtg gggcgacaaa tgaagaatat aatcaaatgc aacagtatta tatggattct
    13561 tcccaaaatg cagcaatcga acaagcgtta aaacttgcag acaagcctta taaaatggaa
    13621 ttcaaaggcg tatatgtcat gagtgtagag aaaacatcga atttctttgg caagctttca
    13681 gttggagata cagtaacaaa agtagacggg aaaagctttc aaagtacaga agcatttatg
    13741 gattatgtaa aaagccaaaa agttggacaa acaatcgaaa tcagttattt gcataatggt
    13801 gaagaaaaaa cagcttctgg tgatctgatc gagttaccaa cagataaaaa ggcaggaata
    13861 ggtatcacgc ttactgacca taccgagatc gaatcagata cagatgttcg tattaattct
    13921 ggctctatcg gtgggccatc agcgggattg atgttcactt tagaaatcta tgaacaagtc
    13981 actggtaaaa acttgcgaca tggtaagcaa attgcaggaa ctggaacgat caacagtgaa
    14041 ggagaagttg gccgaatcgg aggaatcgat aaaaaagtgg caagtgcgga taaagctggc
    14101 gtagagattt tctttgcacc agatgatacg atttcagacg atgttaagaa agaatatcct
    14161 gaaataaaaa ccaactacga agaagcaaaa gctgccgcta aaaaattgaa ttcctcaatg
    14221 aaaatcgtac cagtaaaaac agtacaagaa gcgcttgact atctagagaa aatgaaatga
    14281 aactaaataa ttaaataggg gttgtgacac gagttctgtc acaacccctc tttttcgtca
    14341 aaaatcttca cgataaaaat aaaaacaaaa gaaaaaattg acgtaattag atgttgaagg
    14401 gacgattttg ccagagctca ctgtttcttc aattttaaat gaaaggagta atgaatgatt
    14461 tttactggtc attctttgaa aaaagaacaa tgatagaaaa aatcagcaag aaaatactag
    14521 cttcacccaa attgatcatt tggatgtcta tgatagttct tatcttttgt atcgcaagtg
    14581 gttttttatt atcaaatttg ctattttata gagaaacaga agagactgct gcaaaagaaa
    14641 tactttcttc gtttagttcc agtggagaag atccatcagc tgaaggaaac gatgtagaag
    14701 aggatgctcg tccaaaggtc atgtacaccg atatcaaagg aagtgtgaag gagcctggta
    14761 tctattcctt ttcttcagaa gaacgggtct atgatgtact aaagcgtgca ggtggattat
    14821 tagaagaagc ggatagcgat cgaatcaact tttctgcgaa gatagaagat cagcaagtcc
    14881 tatatatccc tgctgtaggt gaagagctgc cagaacatct tgatcagttg gcatcctcgg
    14941 aaggaaaaca gcctacagca gattccgagc ctcccaaaat aaatatcaat acagccactc
    15001 catcagaact tcagcagatt cctgggatcg gatcagtcaa agcgcaagaa atcattcgat
    15061 ttagagaaga aaacggatcc ttccagaaag tggaagacct gcaggagatt tctggtatcg
    15121 gtgaaaagac agttgaaaaa ctaaaaaact tcgttacaat taaatagaat gattcaaaat
    15181 cgactggcac gagcaataaa tggacgctaa tggatactaa gaataatcca attctaagag
    15241 cttggcagga cgaggaggta gtatttttgg ataggaaata gcttgattgt tcgcaaagaa
    15301 acattaaaaa aaccaatgct gaatgtctat ctccaaaata aaatatctgg gattcatatt
    15361 atgaatacgg ctgtcagcgg caataacagt caagctctac gagaacgctt tgccaaagat
    15421 gtacttagct atacagcaga taaagtattt attctcattg ggacgaatga tctagcagag
    15481 cataaacaac tttctaaaga aacgtaccaa aaaatctgca gtggatgata gttcagctga
    15541 aaaaagacta tagttctgat catgctttct ttatcacgcc accagcagtc gatgagacaa
    15601 aacaaaaata tcggaataac aagttgatca aagaatatat ctctgttctc acagaagtag
    15661 caaaacgcca tgattgtgaa gtatcgattt ttatacggaa tttcttgaac aaatggattc
    15721 gaagaatttt tataatgggc taatggatga cggacttcat ttcggcagtg aagggtagga
    15781 tcttttgtca gacaggatga ttcgttatat ttaaagaata ttttgtttaa gcaatgagta
    15841 ctttttaatc aacttgaaaa aacggaggaa tataaaacat gttagaaaaa tcttcaaaag
    15901 catgcgatac gaataaagaa aaagttactg ataagtcaca taatgaacgc attccttggg
    15961 accagtactt tatggctcag gcagtactgt tatctttacg tagtacgtgt acacgtttag
    16021 aagtaggagc gacactcgtt cgagaaaaaa ggatcattgc tggcggctat aatggagcag
    16081 tcagtggaga tgtccactgc atagatgaag ggtgttacgt cgtggatgga cactgcttgc
    16141 gaacgatcca tgcagagatg aatgcactcc tacagtgtgc aaaacttggt atcccgacgg
    16201 aaggttcaga aatctatgtc acacattttc catgtttggc ctgtacaaag gctttgttgc
    16261 aagctggtgt gaaagggatc aattatctcc atgactatcg aaatgatcct tatgcccaag
    16321 cgttgatcga acaattaggt gtatctgtac accaagttgc tttagactca cagcattttt
    16381 caaaacttca aaatgaatta acaggaaaac atgtctagcg ttttagaaag gcatgttcga
    16441 aatcggttga tttttccggc aattacgtcg atgattatct gtttgttgta ttacactgac
    16501 aattggggtt atcgtttgct tcttgccgga atttttttat ggttttcgct aaaagaagga
    16561 cgttttatcg cgtacttgtg tattttgtgt agcataacgg tatccatttc ttataggcta
    16621 caagagtatt catttttaga ggaaaaaaca acatctgcgc aaattactgt atttacagat
    16681 acgattcgtg caaatggtaa ttttttgaca atggaaggaa ggttatcaga gaataaacaa
    16741 aagctccagc tttcttatca agtaaacaca gaagcagaat tgaaaacatg gcttaacttc
    16801 cgaggaaagg gaatagaatt gactgctgaa ggcgagttca tctctccagc aaaaaagcgg
    16861 aatctttata attttgacca agaagcttac ttcaggaacc atagaatttc tggacggttc
    16921 caaattcaaa agattgaaca gatcaccatg caaaatcact ggcgaaactg gcttcagaga
    16981 aaaagaggat cttttatctc ctttgtccaa tctgaatttc cgcaaaaaac aagtgtatat
    17041 attaattctt tattatgggg ctataaagat gcaggattca gagaaccaga agaaattttt
    17101 agggagagtg gtttgctgca tctgttcagt ctatcgggtc tacacatcca attttattta
    17161 gggtggctgt attatttgtt tcgacgtatc ggatggccac tacacgtcag tattattccg
    17221 ttgagtctac taactatctg ttatgtttct attgcaggaa gttctatttc tgttttgcgt
    17281 gctagtttgc tgtttttatt gcgattgcta gtcaaattgc tgaataagca ttattccacc
    17341 atggacttat ttgcagttgt cttttggctt cttttactat ttgatcctcg atttcttttt
    17401 catgcgggtg gacagcttag ttttttcatg agttttttgt ttatcctgat ttcttttgat
    17461 ggagattggt tgaaaaaaac ggcctctcac gtcctgttta ctatgatcac gatgccgttg
    17521 attgtatggc atttttatga gtggcctatt ttaggtagtc tttttacatt actgattgca
    17581 ccggtattca agtttctttt tcttcctggt gtgttatttc tcacactctt caatcgctgg
    17641 ctgccggaaa ctttactttt gctgatagaa gagggattaa tcttatttga gcgaataatc
    17701 gatttttctt cagggtcgtc tctcattata ggacgtttgc cccttgtgct atctgggatg
    17761 tatattttcg gattactgat ttgtatggat aggttgaaag aacaacctgt gaaacacttg
    17821 ttttatgttg gtcttttttc ttttattttt ttgactttgc catttttacg cttttcttca
    17881 acgatcgctt ttatcgatgt cggtcaagga gacagtatct ttttgcaaag tccttttcga
    17941 aaagaaacga ttttgatcga tactggaggt cagttacact ttgaaagaga aaagtgggcg
    18001 caaggtatga tacgtcctcc tgctgaaaga aatatcgttc catatctaaa aggtcagggg
    18061 ataagtgtaa tcgataaact catcttgaca cacgacgata cggatcatgt gggagaatta
    18121 cccacgttat ctcagcattt tactatcaaa aaaatttata tcggttgggg cgctggacga
    18181 aatgagccat tacaaaggca tttatctgaa gctcaaaaaa atggtactga gatcattgaa
    18241 atcaaacatg gggacaggat cagcggttat tatgatcttt atgtattgac tccttttgaa
    18301 aagggagagg gaagaaatga agattcgatt ggtctatgga tggaatataa caataggcgc
    18361 tttttatttt taggagattt gaatcaagca atggaaaaac aattgctttt gatttatcct
    18421 aatctgaaag ccgatgtagt caaattaggt catcacggta gccgtacgtc aagtaacaca
    18481 gattttcttt cccatattga agcaaaacat gggattatat cctgcggcgt aaataatcgt
    18541 tatggtcacc cgcacagcga agtattggag acactagaag aaaatcagat aaaaacatgg
    18601 cgaacggatc aacaaggaat gatcacctat cgctggcatc cagtttttca tcctcatggg
    18661 ctagtggaaa caatgataga ctagtaattc tcatctgctc atggtaggat aatttttgaa
    18721 agtggtgagt aatttgagta tacaagcaga gttacaaaaa atacgtaaag aaaaacttgc
    18781 atcttgttac ttgattttag gaacagaaaa gtttctccaa gatcaagttc ggacagaaat
    18841 tgtcaaaaaa atccaattag agggcgaaga tgacttaaat tttttaagtt ttgacatgga
    18901 aaatgcaagt ttagatgagg tagtagctga agcagagacg ttgccgtttt tcggagatca
    18961 acggttagta ttcgtagaga atccttattt tttaacaggt gaaaaagtaa acaatggaat
    19021 cgaacaaaat actgatttat tgacggctta tttgaaagaa ccattagaat cgactgtatt
    19081 agtattcttt gcgccatatg aaaagttgga tgaacggaaa aaagtaacga aacaattgaa
    19141 aaaaacagca attatcatca atgtccaaca gttgaatgaa aaagaagtac gtcaatatct
    19201 gatgaatacc ttagagaatt cctcaatcaa aatggaacga agtgcaattg atttattctt
    19261 gaggttgacc gacttagatt tatccaaaat gatgagagag ttacagaaat tggtccttta
    19321 tggacaaaat caacagcaaa tcacggtaag agaagtagaa gaattagttc ctaaaacgtt
    19381 agaacataat atttttgaca tgacccaata tattttaaaa gggaagacgg aacaagcttt
    19441 gcgtttatac gaagatttag ttatgcaagg ggaagaaaca atcaaaatta atgcgatttt
    19501 gctttctcaa ttacgtttat ttttacagac aaaatttctt gtaaagattg gttatcagca
    19561 ggcaaatatt gctgaaacat tgaaaatcca tccatatcgt gtaaaacttg ccatgcagga
    19621 agtaaaaaaa ttcgatgaac atttgctggt acagttgttt gatcaattag ttgagatgga
    19681 ttatcaaatc aaaacgggac aaatcgaaaa agaattaagt tttcaactat tcgttttgcg
    19741 cacagggcag ttgttaagaa atcgataaga agaaagacga gggcaaagca tgcgggaagg
    19801 tcgtttgttt ttgtcctttt tttgacaaat aaaaaaatcg gccgaagccg attcttttat
    19861 ttagcgattt ttttgcttaa acgagatttg tcgcggttag ctttgttttt gtggattaga
    19921 ccttttgatt cagccatatc aatagcttta acagcttctt tatataacgc atctacgttg
    19981 tcagcacctg aagctacagc atcttcaaat tttttgattg cagtacgcat agcgcttgtt
    20041 tgagatgaat ttttcacatt agcgttttcg cttgtacgaa cacgtttgat tgcagattca
    20101 atgtttggca ttagtttcac ctccgataac ttaagttctt acgcgtgtta ccgcataatt
    20161 caacattcat tattatacct aaaggcagcg tgctttgcaa tatgaacatg acaagttttt
    20221 ttccttaaca gtcttttatt aggatttttt ttcagtaaaa acggatagta caagctgaaa
    20281 aaaatcaaaa agacgctatt gtgggaggca atagcgtcaa accaaactag atttttattt
    20341 atctatttaa acaaagtttg aatgggaatg tttggtacta agttattata taacaaagag
    20401 aaccgtttgc aaagtttttt ttgaaaaggg atacgattcc ctgttgttct ttgtttttct
    20461 attaagaaac tgcagttatt ttataaccag tatcctttga tcagtaaagg ctattctggt
    20521 gtaccgatta ttttaatgta ttcatcgata ttttttatca acattttttt tatgaccaac
    20581 tggttccgac ggttccattt gccacttact tccagcacat atttatttag gggcaaacgt
    20641 aagatatatt ttgccaaata gcgatcttta agcaggccat tgatcacacg gtcctcaagc
    20701 tgcaagttga tcccagtcag catcattggc tgttcttgta aaacgtacgt atcaattaaa
    20761 agaccttcaa aagtttccac aagcgtcttc ctttcttttc aaagtaacct gaaaacaggc
    20821 actaattctt ccattttgat attcgttgaa gtggatgcga tcattctttc ttttgtccac
    20881 tgtaatctag tataacaaaa aagagcagaa gagccaaaaa gaaaaaagag catcgaattc
    20941 tataggaagt ctcattggca cggcctaaat tattacaaaa cattgattaa aaaaagcccc
    21001 tgcatcgcag gagcatcccc tatatcgttt taataggtca atcaaaaagc agtatcaaac
    21061 acattttgta tcaaatcagg aaataataga taagctaagc ccagaatcaa aagtaccgct
    21121 agaagaaacc agccgattgt tcctatgaaa tactttaatt tttcgaacat tgtatcacct
    21181 cgttgtcagt atatcacgga tcagtaaatt gggggtgtta agaatctcct aaaatagaca
    21241 aaatttgttc tattttccta aagcaaatac ttgtaagata cagtaagtga taccagaagc
    21301 aatgaccggt ccggcggcga ttcctcttaa gaagacaaca cctaaaatcg tgccgaaaac
    21361 aagtgcaaca gtgatttgtg gatcagaagc aatcaatcct acacccttgg aagataagac
    21421 agccactagc ccaccgcaaa aaacagctac ccagccaata ggggatttaa aggcttctaa
    21481 tagatcacgt agaccgattt ctcctgttgc gatagggatt agaatagccg ctgtgatgat
    21541 cgtcacccca atattgattc ctttgccttg aagccaaggg aaaaaggaac gggaggctgg
    21601 tatgagcttc ataatcaaaa caaaagctgc cgctatcatc agactttgat ttttagctaa
    21661 aattgctaca agtaaaataa ttcctaaaaa aagccaactt tccatgaaat atattcctca
    21721 ctttcttcga aacaatcata acatgttttt agaaagttac aaaagtagga attcatcctt
    21781 caagacctat tcataagctg tttttttcat aaaataaaca aaatcataaa aaaacacttt
    21841 gcttatttga cagaagacag ccgcgctttg tatagtggac aagacttaca tttttttgaa
    21901 gaatcagaaa gatcaggtaa aggtcttagt aaacgcaagc cattcagaat aaccaatatt
    21961 gtgcttcctt cgtgtcctac aactccaaga ggtagattta aaaattgcag gaagttagaa
    22021 gcaatcaaca agagaataac aagaatcgaa aaaataatat tttgtttgat gatccgccgc
    22081 atttttaaag atagccgatg actgtagaca aatttattta agtcgctttt cattaagacg
    22141 atgtctgcga tatccatagc ggcatctgtc ccttgcccca tagcgacacc taaagaagcg
    22201 gctgctaaag caggcgcatc attgactcca tcaccaacca tcgcatttac ttggtaaagt
    22261 tcattctctt ttttgactag ttgtgttttt tcttccggag tacagcttgc atagtaatgt
    22321 tcaatcccta cttgttcagc aatggccttt gctgtgccag aatggtcgcc ggttatcata
    22381 cttgtatgga tgttttggga tttaaagtag gaaattactt gttgggtatt tgcttttgga
    22441 atatctagca gacctaaaac agcaattaac tgctgatctt tagaaagata aatcacggtt
    22501 ttgccttgat tttccaaacg ttcgatcatt tccgcaatct ctttgctgat tatcatcgtc
    22561 tcaggatcat atgcgtgctt gccaactttc cattgcgcac ctaaataggt agtttgtaat
    22621 ccaaaaccag taatttcttc tattggtaag tattggattt cttcaggtat tgtgcaatcg
    22681 aaatattgca aaatcgcatg agcaagtgga tgcgtcgttt tttgttccat agcaacaaga
    22741 agttgttgtg ccaaatcctt ttcatcaaag aaaaagctgt cagttacaac tggttttcct
    22801 ttagtgagcg ttcctgtttt atcaaaggca atcgctttta attcagaaag cttttctaaa
    22861 tggataccgc ctttgattaa aatcccattt tttgcagaat ttgaaagagc agctagagaa
    22921 gctggtgtag cagatgcgac aagcgcacaa ggagaagcca caactaataa taccattcct
    22981 cgataaaagc tttcatccca actccaacct aggaaaaagt gggggagaag gatcattaga
    23041 ggaacagcaa gcaaaatcaa ttttacataa ttattttcga ttcgttcgat aaatgaagct
    23101 gtttgagttg gcgtattctg cgcttcttct actaattgga taatcttagc aaagacagtt
    23161 tcggaactcg tttttgttac tcgaatcgtg atgggatttc cttgattcag cgttcctgca
    23221 tacatctcat cgcctaaata tttttctaca ggcacagatt caccgcttaa tgcagactca
    23281 tcgatcgttg cctgtgtgct ttctaaaaag ccatctatcg gaacagctgc tcctttagcg
    23341 acaaatacca agttatccac ctttagttga gaaacatcta cttcttgcat ttgtccattt
    23401 tcttttaata attgggcttt ttgtggttga agattcatca aagcagtaat ttctttttga
    23461 cttttattag ttgtgtattc ctctaatgca ccgcttaaac aaaagatgaa tgtcaacatt
    23521 gctccttcaa agtaattacc gatcaaacag gcaccaatag cagctaaagc catcaataaa
    23581 tccacattca gtgtccgatc attccacaat tctttcaagc cttccgatgt ttgtttatat
    23641 ccaccgatga taatggcact gatataaaaa agtggggtga tggtaagaga gaatcggtcg
    23701 aagataaaac cgaagatcat aaataaaagg cagccgattg ttgctgcaaa agcttttttc
    23761 tgattagtca tcgttattag acctccttgt caaaacaagg atagcataat aaaaatgtaa
    23821 ttgataataa taacagctaa atgagaacga taatggttat catttggtga ttaagcttga
    23881 tgttgttttt attatttata atgattatta ttaaagaaaa taaaaaacca gaacatttct
    23941 caaataagag aagtgttctg gttttttcta cattgcaata aaccaataac caatcaaaat
    24001 gataccaagg ataatacgat accagccaaa agcggtaaag tcgtttttct tcaaatagct
    24061 tagtaagaat ttgatagcta agacggaaac cacaaaagat acgaatgttc ctacaagaag
    24121 aacaacagtt tcttccattt tgaatgaatt cccttcagct aaatatttga cgatcttcaa
    24181 gccgcttgcg ccaaacatga caggaatccc taagaaaaat gaaaactctg tagcgacaaa
    24241 acgcgaagca ccaatcaaga tagatcctag gatcgttgcg ccagaacggg atgttccggg
    24301 aacaagtgcc aagacttgga ataacccaac aagaagagct gcttggtacg taaagtcatt
    24361 cagagaagtc caacgaggtg ttttgttttt gttgcgtttt tcgacaataa taaaggcaat
    24421 accgtaaacg atcaaaacaa tggcaactgg taagaacttg tggaaatttg cttctaacca
    24481 atcatccaaa ggcaaaccaa tgatgatggc tggtatgcaa gcaacgatca ctttaaacca
    24541 aagtgtccac gtatcttttt tttctaccgt tgatttactt ggagaaaatg ggtttaactt
    24601 gtgaaaataa agaacgacga cagccaagat cgcccctaat tggataacga cattgaacat
    24661 cgacataaat tgtgaactgg catccagttt gatgaattca tctgctagga tcaaatgacc
    24721 agtactactg atgggcagcc attccgttat tccttcaata ataccgagaa tcactgcttt
    24781 gataatattt gcaaaaaaca taaattcctt ccttcccaat taagatacat cttatagtat
    24841 accgttattt tttgagaaac ccaatctgtt ttttaatatt aagaaaaact tgagaaataa
    24901 agcagataag tacacgaaga gaaaaaagaa gacaaagccc tcgttcagac tttgcctttt
    24961 atgattctct ggtaaaaatt aaaatatccc cattatagaa acgagtcgcg actatgtgtt
    25021 ttcccgttat cgatataaga cgtatcattg atgccgtgga tagaaaatcg accgtttgca
    25081 taagtgacct ttgttacgct gccatttaaa agcccagcac gaacaggctg tttaggatcg
    25141 atcagattaa gcaaaaaggc gatcgtcaaa ccatgagata cgactaaaac atttccacca
    25201 ccattttttt cgcagtgatg ggcgatatct tcaaatcctg tccatacacg gtctcgcaat
    25261 gtttcataag tttccgccca cccagcggta tctaagttgt atacagcatt agcaatttgt
    25321 tcaaaagaaa cttctgtggt aaacatttca tcatatgttt taaaattcaa tacacgaggg
    25381 atcacacccc acatttccat gtcatatcca ccttcaagag acccgaaaca ccattctcta
    25441 atcctttttt caataaaata aggaatttct tttcccttag aatgttctcc tagaataata
    25501 cgggcagttt cgattgcacg accactgtca ctagaataag caaatgaaaa gtcgacattt
    25561 tttaacccgc gacctaaata atgaatgcct tcttgtcctt gctttgtgag gggtgtatca
    25621 caccagcctt gtactcgttc aattgtattg aacatcgttt ttccgtgtcg tacgatataa
    25681 agtgtagttt ctgtcatttt attctcctcc taagtcgttt taacgattga aaaaaggatt
    25741 tgtccttttt tcctgttcaa tcgtcgttgc atcgccatgt cctggataga ctgcaaattc
    25801 atcaggtaat acaaacagct gggtccgaat actatgaagt aattgttcta aattccctgt
    25861 atacagatcg gttctaccga tacttcctct aaacagggcg tcgccagaaa cgacaaaatc
    25921 atcaaaaata aagctgacgc ttcctataga atgccctggg gtagggacga cgcgaaaaga
    25981 catgtttcct aaagtataag ttttcatttc aaattcatat tctgcaggcg agacaataac
    26041 atcagcaata tcatcatgtc taccgagtcc agaaagattc aaaatcggat cacctagcca
    26101 tttttgctct agtgggctga cataaactgg gatttgataa tacgcgcgta aaggttccac
    26161 tgcaccgata tgatcgtagt gagtatgggt aagcaaaatc gctaccggct gttggtttgt
    26221 ttttttgatt tcttgtttga ttcgttccgc atcttctcca gggtcaatga tcaaaagtgc
    26281 ttcttcgttg tagaccaaat aacagttttc ttcaatcatt cctgttttta atctttcgat
    26341 atgcatgtgc attcctcctt gatagccaaa acttctttca tccattagta taacgcattc
    26401 attgctagga aacaaaaaca ggtacttatt tctctttact tatctgaatt atcagactat
    26461 atgaaaagta tttggatatt ctaaagagaa ggaaagtaag ttttcatagc attttaatat
    26521 gtccttgtta cattagattc atccaaaaat ccaaattcta tatcctatat ccctgcctaa
    26581 tcagcagggt gtttttttat gtttggaaag agagctcttt ttgctaaact aggttttttc
    26641 aggtaatctt agagagaaag aggtggtgga aatgatcaaa cagtcaaaaa tatctgtgcg
    26701 gcacttagta gaatttattt tgcgacgtgg aagtatcgac aatcgtaaaa aaagtaacca
    26761 tactgcatta gaaggagcga gaatccaccg caagctccaa aaagatgcgg gagaaaacta
    26821 tgaaaaagaa gtctttttaa aaacaacagt tcaattagat gaatattacc tgatcgttga
    26881 aggccgtgca gacggtattt tcaaaagaga cggtatttat tatatagatg aaatcaaaac
    26941 ctccgaacca cgatttgaag atttagaacc agaacaagtg gaacttttct ttcatcaagc
    27001 aagggtgtat gcctatatct atagtcaaga acatgattta caagaaataa atctgcaatt
    27061 gacttacttc cagacggcag aagaagtgat cacgagaaag gttgaacatc agacaagaga
    27121 gcagttagaa acttttttta aaaagctgac tgaagattat aaagaatggc ttgtttttca
    27181 agaaaactgg cgcactgtac gcaatgcctc tttgatggct ttgaaattcc cccatgatga
    27241 gtataggaaa ggtcagcgtg aactagcggt agctgcttat aaaacgatcc gtacgaaaca
    27301 gaaattgttt gtagaagcac ctaccggaac aggaaaaaca atctctacgt tattccccgc
    27361 tttgaaagct gtaggggaag aagagggaga aaagatcttt tatctaacgg ctaaaacaat
    27421 aactagacaa gtagctgaag atgctatgac agcattaaaa gatacaggag ctgaagtaaa
    27481 aagtgtcact ttgacggcga aagacaagat ctgcttttta gatgagacga cctgtaatcc
    27541 agatcagtgc ccatatgcca atggttatta caatcggatt aatgaaggtt tatgggatct
    27601 tttgaatcat gagaatcaga tcactcgtga agtcatcgaa acttatgcaa gaaagcatac
    27661 actttgtcct ttcgaactat cattagatgt cagtatctgg tgtgacgtaa tcattgggga
    27721 ttacaattat ttatttgatc caactgttta tttaagacgt ttttttgaag atgaaaaaaa
    27781 cgaggactat ctgtttttga ttgacgaagc acataatttg gtgaatcgtt cccgagagat
    27841 gtattcggca gaactatctt atgaaaaaac gaaacgaagt aaggaagcta taccaaagga
    27901 attcaaaaaa cttcatcgcc gtttcaacaa actattaaaa gaatttgatt cgatccgaga
    27961 aatagcaaaa gaagatcatt gggattatca tcatcaaaaa gcaccggctg aatctttagt
    28021 aaaggcaggt tatcaattaa gcgaaaaaat taaagaatgg ctggcagaat ttcctgagca
    28081 cccatcacaa gaacaattgc ttccttatta ttttgatttg cttcattttt tgaaggtcag
    28141 tgaattttat gatgatcact acgaaacaac agtcgaaaaa acgtatcgcg atctgatagt
    28201 aaaagagttt tgtattgatc caagcttgtt tcttgagcag tcgttggata aaggaaaaag
    28261 cagtattctt ttttctgcta gtttttcacc actctcttat tatcaggaaa cactgggggg
    28321 acaaaaaagt ctggcttaca aactgccgag tcctttccca gaaggaaacc agcaagtttt
    28381 catcgcaaat tacctagaga cgacttatcg aaaaagggaa cacagtctgg caaaattagt
    28441 agagacgatt catgcttttg ctgatggaaa ggtcgggaat tatttcgttt tctttccttc
    28501 ttatcaatat ttagatcttg cggtggaagc attcagacag caatatcccg acagcaatgt
    28561 gctaatccaa gaaacggaca tgaatgagga agaacgcgaa cgatttttaa caaaatttca
    28621 aacaaatccc gctgaaacat tgattggatt ttgcgtactt ggcgggattt tttcagaagg
    28681 aatcgatttg agaggggacc gattgatcgg cagtatgatc gtaggtgtag gtttgccgca
    28741 gatgaatcat gagcaggaac tgatcaaatc atattatgat gagaaggaac accttggatt
    28801 tgcttacgct tatcagcttc ctggaatgaa caaagtcttg caagctggtg gtcgagtgat
    28861 tcgtggcatg gcggatcaag gaatcgttgt attggcagat caacgatttt ctagtttttc
    28921 ttatcgacgt ttattcccac aacattggca aacagcaaga gaagtacgaa gtgttgggga
    28981 attggaagaa ggaatacagc gtttttggtt atcaaaagaa gcagtaaatc aaaaaaacga
    29041 ggaatgacag acgtcagcct cgttttttga ttaacgatag ttagtgaatt gcaactcgat
    29101 tggtaagtca agtttacgta ataaagcaat cacagcttgt agatcatcac ggttcttacc
    29161 tgtcactcta atctgatctt cttggatttg tgcttttact ttcagttttt catttttgat
    29221 agctgtagtg attttcttcg cgttttcacg atcgattccg ttgaccaaat cggcagtttg
    29281 tcgagctttg ccacctagag cgtgttctga ttcggaaaaa tggatatttt tgattggcac
    29341 atttcttttg atcagcttac caaataatac atctttgatc tgttcgattt tgaaatcatc
    29401 atcgctcaat acaactagat tgctgttctc taatttgatt tctactgtag aatccttgaa
    29461 gtcaaagcga ttggacaatt cttttgttgt ttgctgaatc gcatttttta cttcttctgt
    29521 attgacttct gatacaatgt cgaagctagc atcttttgcc ataggaaccc tcctgatata
    29581 catataaata tttaccatac atctatctta tcataggatt agggcagatt cttacctgtt
    29641 tgctaaaatt cttctcattt gagttcgaag tattttattt taatagataa actgttattc
    29701 ttacatttgg aataccaaag taggggagag gtgcttgtga gtaaaattat gactgcgtat
    29761 cataaaaatg aaagattaaa gaaaatagtg gttattttga taaccggatt gaccgctgcg
    29821 gtcgctttaa attatttttt gattccagcc aatgtctttt ctgcagggat gaatgggatc
    29881 gctcaaatca tttcctcgct tttatatgaa tggtttcata tcaagataga cacaggaaca
    29941 tttatttttc ttttgaatat ccctgtgttt gtattagggt ttctcaaagt aggaaaacga
    30001 gcaacgatcc ttagttttgt gaatgttgta tgtgtatcgg tgatgacgat gctgcttcca
    30061 gtcggacaga tcactgataa tattttgatg aatgccctag taggcggtgt tttattaggt
    30121 cttggcgtag gattatcttt aaaaatgggc tttacaactg gtgggatgga tatcatctct
    30181 cttgtacttt cacaaacaac tggaaaaaca gtcggaaact acatgctgat tctaaatggt
    30241 atcattgtag tcatcgcagg cttcttcttt aactgggaaa gtgccttgta tacgattatt
    30301 tccatttatg caatgactca agtaattgat gccatccaca cgagtcatca aaaagtaaca
    30361 gcaatgatcg ttaccgtcaa gccagaagag gtaacagaag aactagcaaa acggatggta
    30421 cgtggaatga cattactgcc gtctgtcggt ggctattcaa aaaaacaagg gaacatgatc
    30481 atgatggtca tcacccgcta cgaaatgtat gatcttgatc agatcgttca tgaaatcgac
    30541 gacgatgcat ttatcaatat tttaccaact cattcagtat ttggccggtt tgcaaatgaa
    30601 aacgaacaaa aaatgtatcg aacaactggc gtattgccag aaataaaaaa tcgttcacaa
    30661 aaaaaatgag gaatcaggtt tattttagaa taaatttaat ctattacatc taataagaac
    30721 atttttataa atttaaacga gaatcttact tctaaattct catttacaag cagctaaaaa
    30781 aagagtatga taaataaaaa tcgtgttaag gagtgtcgta cgatgagcga agcaaaccaa
    30841 gaacggtctg gacaagaaat agaactaatc aaagaacgta ttcttgctgc cttggaaatg
    30901 gtgattgatc ctgaattagg aatcgacatc gtcaatttag gcttgattta tgatatagaa
    30961 ttcaatcctg aaaatggaga aacagtcatt aagatgactc taacaactat gggctgtcca
    31021 ttagcagata ttttaacaga atctatccat gacgcattaa aagaagtgcc agaagtaacg
    31081 aaagcagaag tcaaactagt ctggtatcca gcatggacga cagataagat gagccgttat
    31141 gcacggattg cgttggggat acgatagaaa tcttggctga ctagctatga gaagcaaata
    31201 ggaaaaacaa aaaagccttt tatcctttct agaagaaaag agataaaagg tttctttgtt
    31261 tataaaattt tgtctgctat attataagtt aattaaataa atgtgttcta ttgataaagg
    31321 acattcaaat ccctttcctt ttggaatcct tcttttttac atcagtttga ttttcgtata
    31381 gtattctttc tcatcttcac gataaatcgc agccccattt ttttgttgtg ctttaagact
    31441 gccaagattt tttttattta cagacaaaag tgcctcatca atgccgattt tttttgcctc
    31501 ttgtagtact aatgctaatc cttgtgtggc atatcctttt ttacgataat taggggagat
    31561 gccgtaacca atatggccag cgccgtttct taatgtttcg ttcagtttat ggcgcaaatt
    31621 aaaaattcct acatactcat gttcatcact ttctaaaata aagacggttg cgggaacgta
    31681 actctcagga agatcgattc ctaaagacgt tttttcgcat ttttttacat aatctaaaaa
    31741 ttcagcaaat gtatagccat aagcattgtt gataaatcca ctctcatttg gtccaaagct
    31801 agtttgtagc tgataagctt tctttaaatc tgcttcccat agtttgatca gtttcatcgt
    31861 attcacctct ttaagtaacc ttattcattt gttttctact atagcactat tgttgtaaat
    31921 agccatgcct atttttattt attgtgtcat atcgtattaa gaatcatcga acaatacaaa
    31981 tatgaattac aataaaaaaa ttatttgaag ttcgttcatt cgttacttat gatctctaga
    32041 agaaagaaaa cgtgaattac ttcagcgtct agaattccag aaggatagta atcaacagaa
    32101 gaaataatca atatccaaat gaaagaagat agctgaatcg agaaaaagag gaaaaaagaa
    32161 atgaagaaaa aaataaaata gttactaatc ccagagtcgt tcgtttaaca ttgaaatcga
    32221 tcatatggat aaaacagatt ttttcaccaa ccctcaatag atgatggagt tgctcggatg
    32281 aattatttgt gtagatggca taagggaggg cgtacagaaa tgaaaaagaa caaaaatatg
    32341 ttcttgacaa aaaacacatg taatcctaaa ctaaaagatg tgatgcggct atggcggaat
    32401 tggcagacgc gctagcttca ggcgctagtg agggtgacct cgtggaagtt cgagtcttct
    32461 tagccgcata atttgttttt ttataaagag aaggtacacc tggagcgatg agaaagaaaa
    32521 taacatatta tctaaaagaa tataggaaaa attttaaaca acggaaccag attcaaataa
    32581 atcaaaaaaa atttgtgtga aggtgggaca gtttaaaacc taatagattg attcaaatag
    32641 tagatttata gattctgata caagaaatac tggcaaacga tagttaagga ataaagacta
    32701 aacttaaact caaaaagagg tcatgcgaaa gaataaatcg catgacctct tactatattt
    32761 tgaatgtgtt taaacgttca aataatgaat cttagtacca accgtttgct tcccagaatg
    32821 ctttcgccgc ttcccaagaa ccataacgtg aagtaacata ttgttctgct actctttctt
    32881 ggtttgcagc agagtagtca ccatttaagt aagaagaatc taattggtaa cggccgatgt
    32941 aacgtccgtt cgttgctgtg taagaaccgc ttgattcttt ttgtgcgatc cattcttttg
    33001 cagagcttgt acttgctgga gtagcagcag tttctgttac agcaggagca ggttgtgttt
    33061 cagcaacagg cgctgcttgt gtttccgttt gaaccgtttc ttgtacaaca ggttgttcag
    33121 cttgaacagg ttcagcttgg actggtgctt cttgaacggc tgcttgaggt tcagcagctg
    33181 cttgtgcaga gccttctgtt gggattgtta attgttgtcc tgagtagatc aggttaatat
    33241 cagaaattga atttgattct gcaatggcat tgatcaagga gttatcacca acgtattttt
    33301 gagaaatcgt tgatagagta tcacctgatt gaacggtata agcttcgtcc gcatgagcag
    33361 tcgttcccat gaagaatgca gcgccggcag ccaaagttgt tccaaaaagt aaagttttaa
    33421 gtgatgtcat gaaaaatctc ctttattcgt taaaaagttt tatttgttgt gttcaacgag
    33481 gaatacttta acacgtgaaa atatttcata ggtaaaagaa gcatgacaat ttatgacaaa
    33541 tccacgtttt ttgtaacaga atgatgttcg ctcgatgtga aaaatgatga ggaatttcac
    33601 ataatgcaga aataatggga ttagaacagg tgttctgatg agaaaagaaa tgtataaata
    33661 acttgaaaga aattgctttt gttcatcatt ttatttacat attgcgtata ttttttgtat
    33721 ttttgcttga gatgttacaa taaaagaaaa aggaggcggt tccaatgagt aaaagatacc
    33781 agaacatcat ggtagcagtt gacggatcaa gacaatcgat caaagcgttc gctgaagccc
    33841 ttgacttagc aaaggacaat gaagcccact tatttatcgt gtcaatcatc aacaaagttg
    33901 agctgaccca tagtgcctat gccttttcaa aaatctatgc agatgaaaaa caaagaacag
    33961 aagtagccat gctgaaaaaa atcaatgatg ccaaagagta cggcattgct gagatccacg
    34021 caatagttga aactggagat ccaagaaatt tgattgccaa tgtcatccct ggacaagaaa
    34081 atattgattt gatcgttatg ggagcgacag gaaaaggtgc tatacaacaa gcattagttg
    34141 gatcgaccgc ttcttatgtc gtatcacatg ccccttgcag tgtgttggtc gtgaagtaat
    34201 gagataaggg aggaatagaa aatggttcgt cctcaagaag tccgtgcgcc aaaagaaaaa
    34261 atagaaattt tagcaattat tgaagacggc acacaaacaa agaagggata ctcgatcgct
    34321 ttagtaaagt ggaaaggaaa aaaaggtgtt gctctgagat gggatggaga taaccaacaa
    34381 gataaaggat tccctgtcac agctaacggt tatcatccag cgtggtttgt tctgcctgat
    34441 aaatttatgg aattatacag ctatgattat gcaagagccg cagcatcaat caaagcatta
    34501 gataaattaa cagaagagca gaatgaaatg gaggaaaaat aaaaaagacg aaccaatcag
    34561 cctttagaaa actgattagt tcgaggagta aaaatgaaaa aaggttattt gggatgaaac
    34621 tagaataatg cgtataactt aaataaaact taaagcaata aaatggcaag agggtgaggc
    34681 aaatggaagc aggaaaagta ttacaggaag taaaagcact atgtgcagat aaaaaatatg
    34741 aagaagcgaa aagcctgatc gaaaagaaca aagaaaattt ggaagaaaag tacccgatcg
    34801 cacaagaatt tatagaatta aaacaaggaa cgatccttca ccgattcaaa catttttttg
    34861 gtataacaga atagaaaaaa cgattaaaga tcggcagaaa tttgctgcat ctttaatcgt
    34921 tttcttttta aacatgcgag ggattcaatt ctgattctta tataaaaaat agatccaaat
    34981 aataagaaaa attactgaaa gcgtcaattc gatgatgtgt gttattaata cagacgttgt
    35041 ttcccaatgc ctaaaagaaa aaatttgata agtaaaatat acagcaggta aagaggccag
    35101 taaaactgaa aacttctttt ttgtccattt tatgtaatag aaaagaatgc ttatcataat
    35161 aaaaaaagga atataaaaaa caaattgacc gatcgcgatc atactattgc ctccgataaa
    35221 taaagtccat gatacaaaat aacactttgt gcgaaatggc gcaagcagtt ttgtggattt
    35281 atccggtaaa atctgtatcg attttgaaat tagaggagaa acttgctata atcaaagtga
    35341 aagaaggagt gggattttca tggatcatgt agtgttagaa atcgatttgc atttgatcaa
    35401 taatattcgg gtgatttact atgttgtaag taacagtgtc gaacaacgtg tactgacgaa
    35461 taaaataaat ggtattttag caaagaaaga cgttcaccga ttcaataatg gtgaaggaag
    35521 ttattatagt atcccagtag aaaaaattat ttatacgaca gtcaaagtca gagaagatct
    35581 agataccaaa aaagcatatg aacctatttt tacaacttat tgattgacag cagatggagg
    35641 aaaaaaatga atccatatga acaacttatt caagatttga tcgatggaaa aatcgaaaag
    35701 attgatgtaa aaagagaaga attaatggct tttcgagaag cgtggctgaa gttggagaat
    35761 cgtaaatatg tggtgggaaa agcctcactg aacggcaatg tgacttatca ttacgatccg
    35821 acacgtttat tttagtatga agtattcaga aaagaaccaa aggaggacga tgcagcagca
    35881 ttgtcctcct ttgattttac aaaagtgaaa gttttactta tttgaataga tttttaaatt
    35941 tgtttttgac actgtcgata tttacatctt ttaaaagatt ttgggctttc tctttataag
    36001 gtcctaattg ttctttatgt tcttcgataa atcgttttgc gccatcgaaa ttgccgttct
    36061 taatcatttc cttgactttt tgaaataatt cttcttgatt cattttgaac acatcctttg
    36121 ctatcaacat gattttttac atggacaaaa cgagaaaaac tgatttatcc acacgtcaat
    36181 aatatcaaat aacgatcatt tttactaaaa aaacactcga ttctcgcttc taaagtagga
    36241 gaaaaatctc caacttggga cggatggaat ttctttaact gacccatata ctgaatatat
    36301 aacaaggagg tagaaagatg aaagtgatta aatatttgat tggactggcg gcgattagtg
    36361 gtattttgct atttggactg aaatattata ctgaaggaag ttatggagaa atccctggta
    36421 tacttgatca gctaaatcca ttagttgaaa aaggagaggt ctatgtaaag acacaaaaac
    36481 cagaggaagt aaatgaatat ggtacagcaa ggtatgtgca gaaagctgct gatgccaatg
    36541 ggaaagaacg aacggttgaa tttaatggtc tttctgtgtt aaaagaaaat cattatttga
    36601 aaattagcaa taaaggagca cacatagaaa cgtatgagga agttcctaga gaaaaagttc
    36661 cggagaaagc attagcaatt attgggtaaa tcagtttttg tattaagaat acgcccgatt
    36721 ttattattca taatttaatt atgtaaacta aatacaagta gtaaaaataa tacaataatc
    36781 cccctgcaaa aagattgttc ttttacaggg ggttcatttt ttcagcaagc acatcaaatt
    36841 gggatggcag ggttcgaacc tacaattgct tgattcagag tcaagtgcct taccagtttg
    36901 gctacatccc atttattcaa caaaataatc atagcacttt cctaaaaaaa cgcaagttat
    36961 ttttcttgat cggatagttg ttgctttact cgtaaaaggc ggcgtactag tttcttttct
    37021 aatccagcag gaagggcaat cagttctgct actgacaaga cggtgtccaa taactgatat
    37081 tctttgacag gttcattcat aatatgatag atgtttgcta aacgttcaat atattcaaag
    37141 cgagtatact ctgttcgagc aattagaaat ttcaataaat cggtggcttt ctcgtactct
    37201 tgatctgcgt aataaacaag agattgacga tacaatttga tttccagttc attgagggta
    37261 gcaggtctgc gacgctcgtt ccactcatta atagagactt taccttgaac atttcccttg
    37321 cagtcacgga caaaaataat cggaatttca tttggatcca tttttttatg tgtgaatttt
    37381 tccattatct acgctccctt caaaggtttg gtatcttgag tataagggat ttttgtttga
    37441 attactagct tttctccaaa gttagcatct gtccacccat tgcagtgaaa atcgccgaaa
    37501 aatcgttgat cggtactgct cgatttttta caccataggg atcgttgaca taaatatttt
    37561 ctttgtcata tccagtgact acacaagcat gaacaagagg agtgactctt acttctccat
    37621 tatttgtggt ccaagtcata aaatcagttt catctgggac ttcaaaattg atagtcacag
    37681 aagtccagac aggatttcct ttagatagtg cttttttgat atcatcgagt tgagcatcgg
    37741 tttttggaaa agtgtgataa tccgtttgga cgatttgctg agcaagggag gcaactgggg
    37801 gaacatagac gaccatagcc cagtctccgc cactgatatc accaacaaat ccttcattag
    37861 gatcgccatg agtcccatct gcattgaaaa cagggacgta atccaattgc tcagctaact
    37921 ggtttttagt tgtttcataa ccataaaaag ataaaagcat agaaagcgaa gtgacttcac
    37981 agccattttc taaagaagga gaatcaaact ggctctctaa tggaacatct agtaaaactt
    38041 ctgctttctc ttttgtcttt ttcgtttttt gtgttgaact tttttgtgca gtgttcattg
    38101 tatgttctgg agaatggata tgtaggtaat aacttgtacc gcttaataaa agagcagctg
    38161 tgccccctag aaacaatagt tttttgaacg tttttttctt catttgaaat ctcccttttc
    38221 agctgtttat ctttcctatt ataaa
//
LOCUS       DAZKLA010000012        37560 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000012 DAZKLA010000000
VERSION     DAZKLA010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.012"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..1595)
                     /locus_tag="ACG85N_000511"
                     /pseudo
     CDS             complement(1..1595)
                     /locus_tag="ACG85N_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1918..2397)
                     /gene="rlmH"
                     /locus_tag="ACG85N_000512"
     CDS             complement(1918..2397)
                     /gene="rlmH"
                     /locus_tag="ACG85N_000512"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFQ0223308.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            2825..4114
                     /locus_tag="ACG85N_000513"
     CDS             2825..4114
                     /locus_tag="ACG85N_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFQ0223309.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4153..4758)
                     /gene="ytpR"
                     /locus_tag="ACG85N_000514"
     CDS             complement(4153..4758)
                     /gene="ytpR"
                     /locus_tag="ACG85N_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFQ0223310.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(4963..5427)
                     /locus_tag="ACG85N_000515"
     CDS             complement(4963..5427)
                     /locus_tag="ACG85N_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0223311.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5449..5769)
                     /locus_tag="ACG85N_000516"
     CDS             complement(5449..5769)
                     /locus_tag="ACG85N_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0223312.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(5913..6992)
                     /gene="pepA"
                     /locus_tag="ACG85N_000517"
     CDS             complement(5913..6992)
                     /gene="pepA"
                     /locus_tag="ACG85N_000517"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFQ0223313.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7192..7542
                     /locus_tag="ACG85N_000518"
     CDS             7192..7542
                     /locus_tag="ACG85N_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0223314.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            7765..8712
                     /locus_tag="ACG85N_000519"
     CDS             7765..8712
                     /locus_tag="ACG85N_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0223315.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(8769..10187)
                     /gene="pepV"
                     /locus_tag="ACG85N_000520"
     CDS             complement(8769..10187)
                     /gene="pepV"
                     /locus_tag="ACG85N_000520"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFQ0223316.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10336..11181
                     /locus_tag="ACG85N_000521"
     CDS             10336..11181
                     /locus_tag="ACG85N_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFQ0223317.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11185..11625
                     /locus_tag="ACG85N_000522"
     CDS             11185..11625
                     /locus_tag="ACG85N_000522"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFQ0223318.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            11675..11869
                     /locus_tag="ACG85N_000523"
     CDS             11675..11869
                     /locus_tag="ACG85N_000523"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223319.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12005..12691
                     /locus_tag="ACG85N_000524"
     CDS             12005..12691
                     /locus_tag="ACG85N_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0223320.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IQEALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13099..13788)
                     /locus_tag="ACG85N_000525"
     CDS             complement(13099..13788)
                     /locus_tag="ACG85N_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223321.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(13763..15892)
                     /locus_tag="ACG85N_000526"
     CDS             complement(13763..15892)
                     /locus_tag="ACG85N_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFQ0223322.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(15940..17277)
                     /locus_tag="ACG85N_000527"
     CDS             complement(15940..17277)
                     /locus_tag="ACG85N_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFQ0223323.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(17529..17888)
                     /gene="rplT"
                     /locus_tag="ACG85N_000528"
     CDS             complement(17529..17888)
                     /gene="rplT"
                     /locus_tag="ACG85N_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFQ0223324.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(17941..18141)
                     /gene="rpmI"
                     /locus_tag="ACG85N_000529"
     CDS             complement(17941..18141)
                     /gene="rpmI"
                     /locus_tag="ACG85N_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFQ0223325.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18213..18737)
                     /gene="infC"
                     /locus_tag="ACG85N_000530"
     CDS             complement(18213..18737)
                     /gene="infC"
                     /locus_tag="ACG85N_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFQ0223326.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(18781..18906)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            19057..19689
                     /locus_tag="ACG85N_000531"
     CDS             19057..19689
                     /locus_tag="ACG85N_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0223327.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(19691..20596)
                     /locus_tag="ACG85N_000532"
     CDS             complement(19691..20596)
                     /locus_tag="ACG85N_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0223328.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(20681..21340)
                     /locus_tag="ACG85N_000533"
     CDS             complement(20681..21340)
                     /locus_tag="ACG85N_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0223329.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            21656..22600
                     /gene="mvk"
                     /locus_tag="ACG85N_000534"
     CDS             21656..22600
                     /gene="mvk"
                     /locus_tag="ACG85N_000534"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFQ0223330.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            22593..23570
                     /gene="mvaD"
                     /locus_tag="ACG85N_000535"
     CDS             22593..23570
                     /gene="mvaD"
                     /locus_tag="ACG85N_000535"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFQ0223331.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            23583..24668
                     /locus_tag="ACG85N_000536"
     CDS             23583..24668
                     /locus_tag="ACG85N_000536"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFQ0223332.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            24665..25729
                     /gene="fni"
                     /locus_tag="ACG85N_000537"
     CDS             24665..25729
                     /gene="fni"
                     /locus_tag="ACG85N_000537"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFQ0223333.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(25978..26781)
                     /locus_tag="ACG85N_000538"
     CDS             complement(25978..26781)
                     /locus_tag="ACG85N_000538"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFQ0223334.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(26801..27643)
                     /gene="kduI"
                     /locus_tag="ACG85N_000539"
     CDS             complement(26801..27643)
                     /gene="kduI"
                     /locus_tag="ACG85N_000539"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFQ0223335.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(27659..28666)
                     /locus_tag="ACG85N_000540"
     CDS             complement(27659..28666)
                     /locus_tag="ACG85N_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFQ0223336.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(28673..29317)
                     /locus_tag="ACG85N_000541"
     CDS             complement(28673..29317)
                     /locus_tag="ACG85N_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFQ0223337.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29417..30175)
                     /locus_tag="ACG85N_000542"
     CDS             complement(29417..30175)
                     /locus_tag="ACG85N_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFQ0223338.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30302..31453)
                     /gene="fucO"
                     /locus_tag="ACG85N_000543"
     CDS             complement(30302..31453)
                     /gene="fucO"
                     /locus_tag="ACG85N_000543"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFQ0223339.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(31535..31846)
                     /gene="rhaM"
                     /locus_tag="ACG85N_000544"
     CDS             complement(31535..31846)
                     /gene="rhaM"
                     /locus_tag="ACG85N_000544"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFQ0223340.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(31848..32693)
                     /gene="rhaD"
                     /locus_tag="ACG85N_000545"
     CDS             complement(31848..32693)
                     /gene="rhaD"
                     /locus_tag="ACG85N_000545"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFQ0223341.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(32707..33963)
                     /gene="rhaA"
                     /locus_tag="ACG85N_000546"
     CDS             complement(32707..33963)
                     /gene="rhaA"
                     /locus_tag="ACG85N_000546"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFQ0223342.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(33960..35420)
                     /gene="rhaB"
                     /locus_tag="ACG85N_000547"
     CDS             complement(33960..35420)
                     /gene="rhaB"
                     /locus_tag="ACG85N_000547"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFQ0223343.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            35573..36442
                     /locus_tag="ACG85N_000548"
     CDS             35573..36442
                     /locus_tag="ACG85N_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0223344.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(36568..37467)
                     /locus_tag="ACG85N_000549"
     CDS             complement(36568..37467)
                     /locus_tag="ACG85N_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0223345.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
BASE COUNT        10915 a         7895 c         6883 g        11867 t
ORIGIN      
        1 aactaattaa aagaaagagg taactacata aactaattaa aaaagctaac ttcatagttg
       61 gaggataaat ggaagaaact tcaatgattg tttctccttg tgttccttca atcgacacta
      121 gtcctattat caatctctct tttttgtcca tttacttttg cgactgttcc ccaataatca
      181 aatacaggaa gcgttatttt ttgtccttta gattcatgta ctgtatagac tattttcgaa
      241 tccatcactt gataaggcat agtatgccat tccttgccaa ttcttacttc atgattctgc
      301 aaatgttctt tgtattgtaa tgcttccgtg ggtgcatagt cataaagtcc tccgggaagg
      361 agtgtcgtgt aacgaataag attttcttga ttcattacca agtgatctct ctgagtgaca
      421 actttatgtt catagtttgc ttttgttgca gcagatacag ccaacagtaa taaaacaatt
      481 gccgttatat taactacttt tctttgctta gctgatgtgc tcttcatgaa aacgaacgat
      541 aaaatcaacg agccaaacag tacttgaaag cctaagattc gccaaggaaa ttgaatcatt
      601 tgaatgggtg tattttgtaa taaagaccat ggaaaaaatt tggtggataa gagccaagag
      661 actaccgcac taaggtaaat cactttttct ttgcgattaa atcctttaaa aaagaagagt
      721 ggacagatca aagaaataat accaatgatt cctaccgtaa aggttgtcag gtcattattc
      781 atcgcaccaa tcaaactatc cgagacattt aatgcttggt gttgtaaggt cgttttatct
      841 ggctcattaa tcgattgaac catgatttgt cggaacatcg gataccaaaa gtatgctgtt
      901 aaaagaatcg tcattcctac tgcctttgac aactccacaa tcaaccggat gctttctttt
      961 tttcttgttt cacttactac aaagccgcct aagactagta agccaacggc tattacactc
     1021 ataaaagtgg aaagaacatg tgtataaatg agtaagctca tactgactgc taatggaatt
     1081 gcacatggct ttttctcata aatcagtgcg taagcataat acataactat cggtataacc
     1141 gtaagtgcaa tcgcttcagc aatagcagaa cgatgataaa tatcaatcgt acggtaccca
     1201 taaaatgtat ataaacagct aaatataaaa gccgcatcga ttctttttaa aaattttagt
     1261 ccataatgaa acatcagcat gatagtacaa acattcaata gccaaacata taagatataa
     1321 ctaacgatta aattattact tatccaataa aaaatgactg ccggaaaaaa ggtcaagtag
     1381 ggataaaaat aattcactcc gctcccataa tgattaaacg tcgtaaaatt gattggacca
     1441 gaaaaaattg aagataaccc tttgactcta gcaaaatgga aggcaatatc ccccgatgta
     1501 gaaatagtat gaacatttct aaaataattg attgaataaa gtccagcaat aattgctagg
     1561 agaatggaca atattatttt tttctttact tgcattactt aattatatac tcctttcatg
     1621 gattgcttta tctagtataa cttaaatttt aaataaatcg ttatttattt ttactctcac
     1681 ttaaaaaagc actaaaatta taagagaggg aggtaatggt ctgaacacca agaattgttc
     1741 caacatcgaa ttgcttaact aatgagttca aagcttaatt atgtggctct tatatttacc
     1801 gagcgtggat tgcgatctct ttagcgaaac aaacatcttt cataaaaaaa acagccatat
     1861 ccgctttctt ggatatgact atttactact actttactat ctttcattct tcttgtctca
     1921 cttatgataa ggcgcccccg cattgatccg gaaggcacgg tagatttgct cgacaaggat
     1981 cagacgcatc aattgatgtg gatacgtcat ttttccaaaa gagatctgag cattgcttct
     2041 tttcatgacg gcttcgctga gtcctaaaga gccgccgata acaaaaacaa ggtggctttt
     2101 tccttggata cctaattggt cgatttgttt agcaaaagat tcacttgtag gatttttccc
     2161 ttcaattgct aaggcataga cgtattcttg ttctttgatt ttcgctaaga tccgttcccc
     2221 ttctttttct tttacttgtc tcatttgcgc ttcgcttaaa ttctccgggg ctttttcgtc
     2281 aggaacttcg atcaattcga tttttgcata ggcattcagc cgtttgacat actcattgat
     2341 accttggatc aggtattttt cttttaattt tcctactgag ataattttga tattcatgtt
     2401 ttctctcctt tggtcttcat gctcactttg tttattataa tcaatttttt cgtctatatg
     2461 tttattttgg ctatttttgt taaaaagatt tccccaacag ttatgcacaa catccacagg
     2521 tttatccaca gaaaacagtg ttattttacc ttatttcatc acattcatta ggcaaacata
     2581 cgttttataa gaatttatcc actttataca caggctgtgg atatgtttta tcccttattt
     2641 ttccaatcct atccgcgtag ttttacacag tatctgtgga aaaagaaaat caaatatttt
     2701 catctgaaaa caatgatttt tcctaaagtt tttcataaaa agttcacagt ctcaaagtag
     2761 actaaagtca acaaataaga ctatactaac agtagataaa tgaatgtaat tggaggagga
     2821 acacatggat agaaaaaatg tgacaccaaa gatgaaaaaa agtaaaaata atagcctctg
     2881 gcgtaagtta ggtctggggc ttgttggagg gatcgttgga ggactactga cagctggcat
     2941 tttttatgca gcgatgggca ctggcaacaa tagttctact gctacaagcg gcaatcagaa
     3001 ttctgccggt gaaacagttg tggaaaatgt caaagtcaat gtagattctg atatcaccaa
     3061 agcggtagat aaagttcaag acgctgtcgt ttctgttatc aacttacaaa gtcaaaacca
     3121 atcaagcggt tttggcggac tattcgggca gcaagaagaa agtagcagct cagatgatag
     3181 taatttggaa gcttatagtg aaggaagcgg cgtgatctac aaaaaagacg ggaatactgc
     3241 ttatgtagtc acgaataatc acgtagtaga tggccagcaa ggtcttgaag tcttgatgaa
     3301 agacggtacg aaggtaaaag ctgaattagt tggaacagat gcttattcag atttagcggt
     3361 gttgaagatc aattcagaca aagtcgaaac agttgcctct tttggtgact caagtgctct
     3421 gaaggttggt gagcctgcga ttgcgattgg ttccccacta ggatctgaat atgcaaattc
     3481 tgtcacttca ggaatcattt cttccttgaa ccgacaagtg acaagtacga atgaatcgaa
     3541 cgaaacagtc aatatcaatg cgatccaaac agatgcagca atcaaccctg gaaattctgg
     3601 tggtccttta gttaatatcg aaggacaagt tatcggtatc aactcaagta agatcgcaag
     3661 tacttctgaa tctacttcta atgtaagcgt agaaggaatg ggatttgcta ttccaagtaa
     3721 cgatgtagtg aatatcatca atcaactgga aaaagacgga aaagtgacac gtccagctct
     3781 aggtatcaca atggtcgact tatctgccgt ttctactcag caacaagaac aaatcttgaa
     3841 gattccttca tctgtaacta acggtgtcat cgtgacaaat gtccaaacag ctacaccggc
     3901 tgaaaaagct ggattgaaac aatacgacgt cattactaag atcgatgaca aagaagtttc
     3961 ttctggtgta gaacttcaat ctgctttgta taagaaaaaa gtgggagatt ctgtcaaaat
     4021 cacttactat gaaggctcga aagaaaaaac aactactgtt gaattgacga tcgatcaatc
     4081 ggcattaaaa caaaatcaaa atagtgaaaa ttaataaaaa agaggttgtg acaaagactt
     4141 gtcacaacct ctttattttg gaaatgcttc tccgactacg gcatcctcag gcaatactaa
     4201 gattcctttt tttgctggag catttggcaa atgcaattca cgagcggaac agatcattcc
     4261 atagctatct acccctcgca aagttcctgg ccagatcatc attccatctg gcatcatagc
     4321 tcctggtttt gcaacgacaa ctttttgtcc agcttcgata ttgggtgcac cgcaaacgat
     4381 ttgcaatact gtaccattgt ctacttctgt ctcagtaatc gataaatggt cagaatcagg
     4441 atgtgggacg catgttttta cgtacccgac aacgatcttc ggttcatgat cagcttcaag
     4501 ctgttcttcg aatcctgctt ctttgatcaa tcgattcaat ttcgctaatt cctcatctgt
     4561 taagcttact tggccgttcc cttcgattgt tagatgatcg gagatatgga aaaagttcca
     4621 agcaacaact tgaccttgtt catctttgat ccgagcaaca ttccatttgc gttcaacagt
     4681 gttttctgtt cctttatcgt cggctataat aaccatcaag gtgtctccga cgtgtgcttt
     4741 gttataagca aaaatcatgt tctttcctcc attatccttt gctgagattt atcttaacaa
     4801 aaaattgcat tcactgcatg aaattttttt catgctttat ttccgaagaa gcctcttctg
     4861 gaacaccatt tattcgtaac aagggactgt gacaagactt ttgtcacagt ccctttactg
     4921 ttttagttaa ttgattaaaa aggtcgtaca agttccactt gcttaattac ttggctgttc
     4981 ttcttcgtca gaaacgatca agacatcaca aggtgaatga cgtatcacgt agctggctac
     5041 acttcctgtc ataaagcgtt cgactgcatt tagacctgac tgcccaacca tgattagatc
     5101 gatctcatat ttttttggca atttgactgc catggattca cgaacagaac catatgcaat
     5161 aattccttct atatttgtgt aatcaacaga tttcccatat tctttacatt cttcgatcat
     5221 ttccttggct gtttcagttt gctgatcgac tagattttga tcaataggtg catagcccat
     5281 aaagttataa aattgctgat cgattacgtt tgctacatat actttcccat tattacggcg
     5341 agccacttct accgcttttt tgaaagcagc aagtgcttgt tcactaccgt cgatacctac
     5401 taaaatattc ttatattctt gattcatctt gacccctcct aacagatgtc agccttttgt
     5461 tacactttct aggaacgatt cgatttcctg ttttgtttta cgatctttat tcactagacg
     5521 acctaattct tttccgtctt ggatcacgac aaaacttggg ataccaaaaa tgttccattc
     5581 agcggctaca tctatgtact tgtcacggtc tacttcaata aattgccaat cagaaaagtc
     5641 attttcaatt tcaggcattt gcggtttgat aaaacggcag tctccgcacc agccggcagt
     5701 aaagaaaaat acactctttc cagtttctac gtagcctgct aattcttcta aactatttgg
     5761 gataatcatt ctttgtcctc tactttctgt ttatgtattt ttcaattaaa ttatacacct
     5821 ttctttcgaa ctaacaaaca ttgatcctct ttttataaaa aaaatctggc aaaaagcaat
     5881 cttttgccag attttatagt ttcactatcg cattattttc cgtatacgat cgtatttact
     5941 gttgatttat ccaaaccttt cagtgtttga atcaacattt ctcgtgcagc ttcaaaatcg
     6001 cgaatactga acatcgtttg atgcgtatgg atataacgtc ccactacgcc aatcactgta
     6061 cttgggattc cttcgttttg tgtatgagca gcacctgcat ctgtaccgcc ttttgaaaca
     6121 aagtattgat aagggatatt gtttgtttca gcgatatcca gcaagtactc gcgcaaacga
     6181 ggaagcatga tcaaacctgg atcttggata cgcatcaatg taccttctcc taaatgtccg
     6241 aatgtgccat ttttcgtcac tgtatcatct gctgcagagc agtctactgc aaagaacaga
     6301 tctggcttga atttattgac agaagcttta gaaccacgca agcctacttc ttcctggaca
     6361 ttcgcgcctg caatcaatgt atgacctaat gtttcatttt gcaacgcttc taaagcttct
     6421 aataccattg tacagccata gcggttgtcc catgatttac tgataatatt ctttccgttg
     6481 gctgttttga tcgtttcagc gtaaggaacg attgtgtctc ctggaaggac accgtaactc
     6541 attgcctctt ctcttgattc aaacccagca tcgaacaaaa tatcggttac ttctactgat
     6601 ttttgtccgc ttgttcctcg caataaatgt gggggaacgg aagaagaaat acatggataa
     6661 ttcttaccag tagaggtttt aagtgtaaaa cgctgagcag aaacaacgta aggattccag
     6721 ccgcctagag gtacgacttt aaaaagcccg ttttcttgga tttgtgtcag catgaatccg
     6781 acttcatcca tatgtgctgc gaccattaca cggggtgcat ccgcttcttc atgatggcgc
     6841 agaccaaaaa tgccgcccaa accatctaac tgtaactcat ctaccagcgg ttccatatga
     6901 ctttccatat aagcgcgaac atcttgttca aaaccgcttg ttccttgtaa ttctgttaac
     6961 tctttgattc gttgaaatgt tttttcttcc atatgctatt cgtctaaatt tcagactaaa
     7021 ctcctctcag acttatattg atctgcggat aaaatatgat tttttctgca gaccgatcgt
     7081 atttattata gcttattttt tctggtagcg gttatttatt tcaaagaaaa tatgagagtg
     7141 tggtaaaata agcacaagga gtaacaagtg agaagaagga ggtatgtcca catgtatgaa
     7201 gaaagcgaac taagctattt taaaggcggg ttagcagttg gaatcggtct aggattattg
     7261 agcggggtag cttcaacgat ttggtatcat cgcaaaaaag tattgtcagc agaccaagta
     7321 cttgaaaata tcaagtcatc ttttctaaaa gaaggtgcaa tcgaaggttc ttggatctct
     7381 tttgaaaagg aacctattag gaaattcgcc gttcggtcga gtgcttatcg gggaggtctt
     7441 tctcgaatag aagatggcga aactgtttat tatgaatttt tagcagatgc tcatacagga
     7501 accgtattag aaatttcaag aaaaaaaggt gcaacagatt aatacgtcaa aaaagtgaga
     7561 gaacgaatac gcacccacta ttcaaaagag aggtatcatg tccttctttt gtagtgggtg
     7621 gttttttaga tagagctttc caaacatata ttccagtatt ttctttttaa ataaatgata
     7681 tttagcggga aaaactagca tttcctttga ttatgaggta tttttaattt agcttttgtt
     7741 ttctatagtt aaggagagtg aaaaatggaa caacaagaaa aaattgctgt tctgattcct
     7801 tgttacaatg aggaagcaac aatttcaaca gtcattgcag actttaaacg agaattacca
     7861 gaagcagata tttatgtcta tgataataat tcgaccgatc gaacatacga attggccgtt
     7921 caaggtggag caatcgtaaa aaaagaacct cgtcaaggaa aaggaaatgt gatccgccaa
     7981 atgttctttg atatcgatgc cgactattat ttaatggtag atggcgatga tacttatcct
     8041 gcagaagctg tacatggttt gctcgagaaa ttacgttctg gtgaagcaga tatggtcatt
     8101 ggtgatcgcc tatcaaatgg aacctacttt gatgagaata agagaccttt ccacgatttt
     8161 ggaaataacc ttgttagaaa tacaattact agaatgtata aaacaaaaat tcttgatgtc
     8221 atgaccggct atcgaggatt caatcgtatc tttgtcaaaa gttttcctat tatgagttct
     8281 ggttttcaaa tcgaaacgga attgacgatc catgctttag ataaaaaatt caagttcgtt
     8341 gaaatcccaa tcgattatcg tgatcgtcct gaaggcagcg aatcaaaatt gaatacattc
     8401 tctgacggat tcaaagtgat catgatgatt attaaaatgt ttaaagatta taagcctttg
     8461 cttttctttg ggatttggac catcttcttc tttttatttg gattggtcac ggggattcct
     8521 gtgattcgcg aatatatgct gacaagcttt attacacgca tcccttctgc gattttaagt
     8581 actggattga tgactttagc acttctttca ttagtaacag ggttgatttt ggatacagtc
     8641 gtcacgaacg ctaagaaaga atatgaattg aatctatatc atgtttatca tgaataccac
     8701 agtagaaagt aaaaaaagga tcaagactgc aaaaataatt tgcaattctt gatcctcttt
     8761 ttaattaatt atttcgccaa acgatagatg gcatctgcat agattgctgt tgctctgaac
     8821 aagtcatcaa gactcataaa ttcgtttgct tgatgcattg tatcagtata tcctgggaac
     8881 atcgctccat aagcaacgcc acgtttcaac aaacgtccgt aagttccgcc accgatgatt
     8941 tgttcgtagc ctttttctcc agtgtggtct tcatagactt gcaataatgt agccaccaat
     9001 ggatcatcca ttggtacgta atgtggttcc attacgcgag cacctcgagt aactgttgcg
     9061 ccttcttgcc ccactgtttg ttcgatgtca ctttgcaatc cgtctactgt tacacctttt
     9121 ggaaaacgga aattcatatt gatgaagttc gcttcctctg taccattttc ttcaaaagca
     9181 aatagtccag cattcattgt caatttgccc attttttcat cttcataagc gactcctagt
     9241 ttttctccat aaaaatcttc atggacataa gcagctgcta catggataaa ttgtttagca
     9301 gatcctagga attcatagtt atctaagaac aatgccaaga aagaacctgc attgatacca
     9361 gattgtgggc ttgcaccatg tgcgccttta ccaactaatt cgatttttac atacgtattg
     9421 tccgcttcaa tcgttccgct tactggattt gcttcgatga attggtaaaa tgcttcttcc
     9481 atcgcgatag ctgcatctgc acttggtact tgaagtgctg cagttgctgt tcctggcacc
     9541 atattttcgc gaagtccgga aacaaaagat ttcaagacat aatctcctgc attgtcgcct
     9601 ttgaagcgta aagctaaaga aacatttcct ttttcaccat tgatgattgg gaattcagca
     9661 tctggtgaga aaccaaaatc tggttcttct tcatgttcga aataataagc catatcgccc
     9721 cagccgcttt cttcatcgct gccgactacg aagcgaactt tttttgatag tttcaagtca
     9781 agttctttaa tgatcttcat cgcatagtaa gctgccatac ttggaccttt gtcatcgctt
     9841 gatccgcgtg caaagatctt tccatccttg attactggtt cataaggatc tgtgtcccag
     9901 ccatctcctg caggaacgac atccatgtgt ccaaatatcc ctagcgtttc gtctccttcg
     9961 ccaagatcaa tatgccctgc atagttatcg acatttttta ctttaaatcc atcacgttcg
    10021 ccataagcta acatatgttt caatgcgtcg cgaggtccag gtccaaatgg tgcatctggt
    10081 gttacttttg agtcatcgcg ctcgctgttg acacgtaaaa gatttttcaa gtcttcaagc
    10141 aagtcttctt tacgtgcttc tacttctttt tgccaatcaa ttgtcataaa gttaatcctc
    10201 cacttattta agtcttcgtg ttcatgatac cacaaattac agaaaaaaat agttcaaaaa
    10261 atctgacaat tccttttctt atctgttttt ctctaaaaaa aatatacgct ataatgagta
    10321 aaaggaggtt tttagatgct gataaacgaa caactagtga aaagagtcat cacacctcgt
    10381 ccaaaaattt cacataaagg aacatttgga cgtatcgttt tgattggcgg caatgaacaa
    10441 tatggcggag caatcattat gagtgcttta gctgccgtac acagcggagc gggtctcacg
    10501 actgtagtaa ctgatgaaaa aaatcggagt gctcttcaca gtcatctgcc agaagcaatg
    10561 attgtagttt ggacggatac tgaccagttt ctttccgtaa taaagcaagc agatgtactc
    10621 ttgattggtc ccggattggg tacttcttct aagagtttgg aacgtttcaa acaagtacta
    10681 gaaaaccaat caaaagaaca atggctggtg attgatggct ctgctttaag tttacttgct
    10741 gctaatcatc ttttgctacc ctatcccgaa caaactgtac ttacacctca tcaaatggag
    10801 tggcaacggt tgagttctct accgatacaa gaacaacggg ataaagaaaa caaaaagaag
    10861 caacaggaaa tcggtgctgt gctcgttttg aaaagtcatg agacaaaaat ttatggtcac
    10921 cagcacttct acaccaatcc tttaggttct gctgctatgg caacgggcgg catgggtgac
    10981 actttagctg ggatgatcac aggattttta gcccagtttc ctcaaaatga ccaaacgatt
    11041 gcagcagcta tttatcttca tagtttcatt ggagaccagc tggcaaaaga acaatatatt
    11101 gttttgccga cacaaatcag cgaagcactt ccgaaatgga tgaattattt ttgtaaaaaa
    11161 tcaatggaaa acgaggcatg agtcatggag aaatttacag gttatgtgcg agaacctttt
    11221 tattatgaaa cagatcaaat ggggattatt catcacagca attatatccg ctggtttgaa
    11281 gaggctcggg tagccttatt ggtacatcta gggtttccct ataaaaaaat cgaagaatct
    11341 gggatcatca tccccgttct ggaagtaaat tgcagctata aagaaatgat ccattacgga
    11401 gaaaaagtga gcatccatcc aactatccaa aagtatacgg ggacacggtt ggattttagc
    11461 tacgaaattg tgggaactga agacaaaaaa ttaaaaacta ctggcagtag taaacactgt
    11521 tttttacttt ctgattcgca acgattagtg aagctgagca aagtcaatcc ggagttagat
    11581 cagttattcc aagcgtacgc ttctttggat gatgtaaatt cttaataaag accgtctaag
    11641 catttttctt gtattgtgtc gagtagttcg tataatgaaa agaaaaaagc aggtgatccc
    11701 catgtatgtc gttaaagtaa tgcacggtta catcgataaa actggctgtc gcactcggga
    11761 aaaaaactta gacaatctgc tcattttcaa agacaaaaaa gaatccgaag cttttgctaa
    11821 aagaatcggc ggtcgtgtca aaccgatcca agaggtacga ccagattgat tcaatatttt
    11881 tcttttcgag gctctaatag gcatgcctat cagggctttt tttgcagact gtgtttttat
    11941 gcccacacga tttgtaagcg atttatgtta taatagatta tatctgcata gaaaggaagg
    12001 ttaaatggtt atcaataatt tgcaacagca agcttatgaa tctatacgta aacaaatcat
    12061 ttattccgag ttagcacctg gaagtaaaat ctccgataaa gtgttagaag aacaactagc
    12121 tatcgggcgt acaccgattc aagaagcact gatccaactg cgcaatcaag agcttgtaaa
    12181 aaccattcca cagtccggca cttatgtttc aaaaatcgat atccgatctg cttcattagc
    12241 ccgttatacg cgggaaaagt tggaaaaacc gatcctgcaa gaatgcagtg cgaaaatgac
    12301 caagcaagat caagctaaac tagaaaaaat actagaacaa accgatcagg cagtaatcgc
    12361 tcaaaataaa aaagcttttt ttgatttgga tactgatttt caccgaactt gttatgaaat
    12421 tgctggaaaa cgagaaattt gggattggct agaaagttat agtacgcact tgaaccgttt
    12481 cagatggtta cgtttgacga tttcagaatt agactggggt cgagtattag atgaacatca
    12541 gacaatgctt caatcaatga ttgatcataa ctttgatgaa gtcggatttc tttgcacgat
    12601 gcatttacat atgattatcg aggagcaaga atacgtgatt cataattacc ccgattattt
    12661 tgaagatatt caggacagac ccatcaaata agaggttttg gaatcagcgt tttgacctga
    12721 gtattagaga aagaaacggc aaaatagttc ctcatatttt gccgtttttt ttgagctaat
    12781 accgagggtc agctgattgc acaccgttta ttgagaggtt gtgaaaaagc tgccctgcat
    12841 caagtaataa gaacagccaa gtaaaaatag cttcttatgt ttttaacttg gctggactta
    12901 ttaccgcaga tgcaagcttt tgaacaccgt tttccaagag gtcgtgagcc tgccgttcat
    12961 ctccgagtca caaggaacaa tttcaaaaaa cagcttctca tgtttgttga atattttgac
    13021 ttgtgaccga ggagttgaca ggagaacatc gtttattcgg aaaaaaagga ctgtgacacg
    13081 acttttgtca cagtctcttt atttgtaatc taaatcatac tccgtacatc ttttttctat
    13141 tttttttatt tcttctaaga tcccttggct acgaatttca ttatatggcc aaagtaagac
    13201 tgcctctaat acataaggcg aaaaatcgct ttgattcatc tcttttgaaa aactcatcgc
    13261 tgctatctcg ctcaatgcac gaatgtttga ttgatgtgta taaaaaggca accgccacag
    13321 cacaattttt tcgctttgcg tataaattcc cttaacagaa gcttcgatgt ggtgaaatag
    13381 ttcatgtcca ataatcaaag cttgcgctgt ttctttagag atcgaagcta gtcctgaaca
    13441 ttttaatttt tctaacggct ccgtcatcaa tcgaatccct tcatcttctg cataagttgc
    13501 aaaagtgatt cgttcgttta tttgctctga ttcacaggaa acgattggaa cagccatttg
    13561 ttcacaaatt tctcctacat cggcaaaagg gtactttttt ttgatgctac atgccgtttc
    13621 gataccacaa tcgattgatt gttgtataag aattttctgc ttggacaaag gaatcttttt
    13681 ccaaaataaa tctcgccgaa actggtagaa tccccaaatt tcttcgggga tctgttgtaa
    13741 ttcgtttagt aactcttcta acttatgttt gtttgatatt tccggcaacg acgatcactt
    13801 cttcctccgg ttccatagta gatacttcca gatccatcaa aagctgtagt tgttccagat
    13861 ccgtcgctgt aaaatcagag actcttgctc ctaagcagag ataaaattct ggtcgggcaa
    13921 tcaattcagt ttgataaact gccatatctt cccaaagttg ttcgacagct gtcatataat
    13981 tggctgcagt tgttttcata catgaagcat gtccacgttg gaccttgata aatccttttt
    14041 gatcgatgat ccgaagactt ccagcatttt ttgaacgatt ctgttcgccg catacataaa
    14101 agaaaggtgt attttccaat aagcaaactt catttttatt taatcgcata tcctctgcag
    14161 caagctctag tgcttcttct gttgtgatct ccttggttag atcggttgct ttcacttccg
    14221 tagaaccagt agcgatagcc gttacttttg aagtctgtgg atcgatttct acgtggactt
    14281 cgatcgattc tggtgtagcg cctgattcaa tcgctttatt catcgcttca tttttcaacg
    14341 atcgtatatc ttctttactt ggcgaaggga tgatacgttc tacaacatct cggaccattg
    14401 caagtgctac accaatagaa gaaatcactt cggcgttttc aggaatcgaa tatttcacac
    14461 ccattttatt actaaagtaa gtaatcaatg aggcagcgcc gccccctaca cctacaagag
    14521 aaatctgatc tttctccaag tgatacttgt ctgccaattc taaaatcacc ggttcaatct
    14581 tagcatagga tttttccatg atctgttctg cgatgtcttc aaccgtcgta tgacaataat
    14641 cagctaatgc ttggatggct tttcgtgcag atgggacatt tccataagaa aaatgttctt
    14701 cttgcactaa tccaaggaca ttggctgcac atgtatttgt aatagtcact tcttctccgt
    14761 cttccatcac taccttgacg taatcagcag ggtctccttc tttaggagag aaaaattcaa
    14821 ccttaggacc tttgattttt tctgtctccg taaatactgc ataatctaga ccggcaatat
    14881 gtgcagaacg tggaccgaca tcgatgattc ctgactgatt agcccgtacc atcgaaccgc
    14941 ctgccacacc tagaacccga acatctaatg aagagatata tgttgggtgg ccacctacaa
    15001 ttgaataatc gattgctgga cgtccatttt taatgacccc gatattggtt gttgttccgc
    15061 ctacttcaaa ataaacacca ttagacgctc tcaagtacat caaagagccc atgactgatg
    15121 ctgctggacc ggaaagcatg gttaaaacag gtcgtttttt catttcattg atttccatta
    15181 ctccgccgtc tccgcgcata atcatcaaag aaacattgac ccctgcttct cgtacggaat
    15241 cttctgtaga ggttgccgta tctagcattt ttggtaaaat agatgcattg attgctgctg
    15301 tccgagttct tcttgtcaat ccatataatt ttgtgatgtc agaagccatc gttgtaggga
    15361 tcgattttac cgatgctgct tcataaacaa ctcgttctgg ttccccatta tctactccaa
    15421 atgccataga agaaaccaag acctccgctc tttctctttc taaagaagaa atcgtttctg
    15481 aaacacggtc tacatttaag tgtttgacag gtaagaatgc attgacaatc tctatttttt
    15541 tcttattccc taaatcgata tcgttcaatc tagtttgtcg ttttgctaaa aaaccttcta
    15601 atccaccttt tgccatcccg attacgccga cttttgctac gtctccttca atcagtgcat
    15661 tagtcgcttg tgtggtagaa tgtgcaacaa acacaacatc ttctggactg atattatttt
    15721 ccagcaaaca attttgaaaa gactgaacga cacctgcggc tactccacgg acatcatcgt
    15781 gggttgtttt cactgaagat tttccgatga tttcgtgcgt cgcattatcg attgcgactg
    15841 ctttcgtatg tgtaccgcca acatcaattc ccattcgcac aaaacgtttc attttctttt
    15901 cctccattca tctaatcgtt tttatcgacg ttttgtttac taggcgttcc cgtacaataa
    15961 gaacgtgatc aggcataaga tcgctccgat gatccaacca gttgggatgg atttcttcat
    16021 atattctcga gaagttactt ttgtataacc taatccccaa gcaacccaag attgtgtaat
    16081 atctaaatgc tgaggagcaa tcgttgtgat tgcaaataac gggaacaaaa aggctggcga
    16141 catcgttgga ttaacagcta atacgacagc taaaatagct gagcctgaac cgactagatt
    16201 cattggaccg cggaaaaatc ctagtggtgt caatatagca aatacgattg ccaagactaa
    16261 tggtgattgc ggtatccaat ctccaaaaat agctgaaaaa taaggagaag catacgcagc
    16321 tgaattattg aacatcgcta aggtcatcaa gaaaccaacc attggcgcaa catctactgc
    16381 gccatctgta aataatttcg caaactgacg acagatttct ttataacctc cgcgcaattt
    16441 tccagtaaac agcaatgccc agatacctgc tagaataaaa cctaagatga ttggaagatc
    16501 taaaagaacg acccctaaaa ctggtaataa tacggctaac catgagatca ttggtgcgtc
    16561 gccaccttca gctggtacgt ttgctgccat tgcatagcgt ttctttttgt tcattgatat
    16621 attagcgacc gttagtacca caactaagct tacaatcatc ccaatcatac caatttggaa
    16681 atagtcatta taagtatagc tttcagccgc tggattgaaa ccagcataaa tcgtttgata
    16741 ttgtttgaaa ttgacgatat tagcaaagat tccagccata atcgagccca taaatgagaa
    16801 cagtgcaacc ggtactgaaa tacctaatga catcaagatt ggcaacacaa taactgcaat
    16861 agaaatcact ggtccgattc ctgtcatcga catgaatata acagcggtta caatacatag
    16921 taagctcatc gtgattcttg gcttgtcccc tccaagttca actacctttc gaatcagggt
    16981 agctgcgatt ccgctatcta ctagaacacg cccgaaaaag gcaccaaaga agacatttac
    17041 caagatcgat ttggcgtact cagctggccc cgtcgtatag atatgcgtca acacatcaat
    17101 tactccttgc ccttccatcg ccgggtttgg tgaaaacgtg tttccgacta gcgctaatcc
    17161 catccataca gtagccatga tggcaaagcc gaccatcaag ttgtagcctt ttacacagta
    17221 ccagaccatg aaaaagaatg agataactaa caaaatacca ataataatat cactcatgtt
    17281 attcctccgt ccattttgtt tttctgataa gcaaactaat atatcagtca aactcattct
    17341 aacttatgta gttagcgctt tcattaaagg gtgaccataa atgttcagtt ttcaattaaa
    17401 aaaccttaat tatgctattt tagacttata cgttattgtc ctgtttcatt tgatggagga
    17461 ttgtaaataa aaaaagagga aagaacataa cttttcgtta tcttctttcc tctttataat
    17521 tgtttagctt atttagccaa agcttctttt gcttggtcag ccaatgcagt aaatgctgct
    17581 gcatcgttaa cagccaagtc agctaacatt ttacggttga tgtcgatttc agcaactttc
    17641 aaaccgtgca ttaattttga gtagctcaag ccattcatac gagctgctgc gttgatacga
    17701 gcaatccaca atttacggaa gtcgcgtttc ttttgacgac gatcgcgata tgcgtagttg
    17761 tatgaattca ttacttgttc ttttgctgtt ttaaataaag tgtgttttga accgtagtaa
    17821 ccttttgcta attttagcac tcttttacga cgtttacgag ttactgttcc acctttaaca
    17881 cgtgccatgt ataattcctc ctaataaaaa taaatcttca gattctgtta tttgtacttc
    17941 ttatttcatt tttgctagtt gttggcgaat acgtttgaag tcgccactag aaaccatgct
    18001 tgctttacgc aattgacggc gttgtttttt tgttttgccg tggaaacggt gacttgtaaa
    18061 cgcgcggaag cgtttcaatc cgcctttacc agtacgtttg aaacgttttg ctgatccgcg
    18121 gtgtgttttt tgttttggca tgactatatt tcctcctcaa aatctttcgt tatccgacta
    18181 tcggctgtcc aacagccagt tagtcattca aattacttac tgtcgttttt cggtgccagc
    18241 gtcaagaaca tgctgcgtcc gtccattttc gctttttgtt ccactgttgc aatatccgca
    18301 gtttcttcag ctaagcgatt caagactttt tgaccaattt ctttatgggt aatggcacgg
    18361 cctttgaaac ggatagaagc tttcacttta tcgccctttt ccaagaactt acgtgcatta
    18421 cgaagttttg tattgaagtc attcaagtcg atcgtaggac ttaagcgaac ttcttttaca
    18481 ttgatcactt tttgtttttt acgcgcttcg cggtctttct tttgttgttc gaaacggtat
    18541 ttaccgtagt ccatgattcg cgcaactggt ggtttcgcac caggggcaac taacacaaga
    18601 tccaagtttg cttgttctgc aatattcaat gcttctgctt ttgtcttaac tcctaattgc
    18661 tcaccatctg aaccgatcaa tcgtaactct cgtgcacgaa tgccgtcgtt aaccatcata
    18721 tcctttgcta tggtcattca cctccaaata ttatgagaga aagaaaagct taccgaataa
    18781 acggcaataa aaaaacgagt tctcttacag aacccgcgcg tacttcacct aatagaacca
    18841 gtgaaaacta tctagcccag tgacattatc aactaaggcg agaagcgggt gcttctgctt
    18901 ttatttctca accatcatag tatagcaaaa actaatcttt ttgtcaatgt ttttcataca
    18961 gtttgtcttc cttttctgaa aatgattaga ttagcagact ttcttgcagt gattcccgct
    19021 tctttgctat actaaagaaa aacgaggtga acccacatga aaaaaatcga acctattcca
    19081 ccttctcttc ctcttcttac aaagggagag ttttatttag aagatgagga gatcttctct
    19141 ggtcttctga tcaacggggg tgaccaaagc tatctcgatg ccaaaaatat ggtattaaga
    19201 gagagtcata tccaaaaact tacgatgcaa aaaacagttt tacgtcattt tgaatgcagt
    19261 aatgtcattt ttgaaaaatg tgatttttcc aatctggagt ggattggtgg cagtttccac
    19321 caagtcgtgt ttcatcaatg taaattaaca ggtacgaatt ttgccgaaag ttatcttcga
    19381 gactgtactt tcactgattg tattgcttcg atggcttcct ttagtagtac aaacttgaaa
    19441 gctgtctcct tcgatcattg tcaattagaa gacagcgaat tttatgaggt cacttggaaa
    19501 aacctttttt tgtctgacaa tcagttgacc ggttcgaact ggtttcgaac ttctctgaag
    19561 ggattagact ttacgactaa tttcttcaca gcaatcgctt tatctcaaga ttctttggcg
    19621 ggactaatcg tcaaccaaga gcaagcactt gtgatcgccc aagcccttgg tttgcagatc
    19681 aaagactaga tcaacgaatc cgccgccaca gtgcaataag catccaaata gataagacca
    19741 aactgatgat caaggcgatt ccccatccga aaacggaatg ggtaaagggt aactggagat
    19801 tcattccgaa gaatcctgtc acaatagttg gaacggtcaa gatcagcgac cagaccgtca
    19861 aaaatttcat cgtgtcattc aagttgttat tcagcaaatt attataagtg ccagagagct
    19921 ggtctaaaat ctgcgaagct aagttcgtca tttcaactgc ttgtcgtgct tcgatcagcg
    19981 catcgtccaa ctcttctttt tccttttctg acaacttgcg ataaatagcc aatgccttga
    20041 tttgttctaa aagtactaca ttttgttttg ttcctgtaac taagtaaacc aaaccgatct
    20101 ctaaatctga aagttgcagc agattcttgt tcgttgtttt ttctcttaat ttcagattaa
    20161 gccgctgacg ttcactattc acttcctcga ttaaaggaaa aaaggaatca gaaatcaaaa
    20221 acaatgtacg aaacaataaa ctgtatacac tcatgtctgg cattcgttcc aaaagcgatc
    20281 gaattagtcg aatcacgtac tcggtattcg gcgtggtaaa tgtaaacaaa ccgtcttctt
    20341 ttaaaataaa agcgatagga ctagtttcat aatgattatc gactttttgc tggtgcggaa
    20401 catttgatac aagcagtaac gcttcatcaa aagaatcgta ctctacgcgc gcccgttcat
    20461 ttttatctag agaataagtc agcatctcgt cactaatgcc atattcttct tttaagtttt
    20521 taacagctag cacatcgttt tgctgtacat tgatccaaaa attacggtct tgattaaaat
    20581 agtgtttttc ccgcatactc tatcctcctg acatttgttt ccagtataaa gaaaaaagtc
    20641 gctcaaataa aggtttaccc tttatttgag caactgtaaa tcaaatggct actttggttt
    20701 ggcgaatctc tgctaatttt tcttcgatca ttttcaagac ttcttctttt gcttgaggat
    20761 cttccacaaa attatagcgg tctccatcga tttggatttt tggactttct ttgtattctt
    20821 cataccattg atcgtaacgt ctgttcaatt ctttgtaata atcgtataga cttggatcaa
    20881 aggacaattg ttcatacgaa cgaccacgtt tttcgatacg ctccaacatc gtatcaaaag
    20941 atacacgaat atgaactaat aaatccggat gttttttatg ggcagcatag ggaagttctt
    21001 ccatcatgtt ttccagcagt tcatcataca cacgaacttc tgtttctgtt gctcttccta
    21061 gatctgcatt tagatggaaa agcagtgaat cttcataaat cgaacggtcc aatacattat
    21121 tgtcatcttt cattgcttgt ttgatgcttg caaaacgttt attcaaaaaa taaatttgca
    21181 gcaaaaaagc atactgttca gggtttgcat aaaataaggg caagacttca ttatcatcaa
    21241 tcgattcata aaatgcctga ctatcaaagt gttcagccat catttctgtt aaacttgatt
    21301 ttccggcacc aattgtacct gctaaaacga tcacgctcat ttacctccag ctcacttcat
    21361 cactaaatat tgtgtttaac tccaaccttt ttaaatatac ccccaatatc tagtagtgtc
    21421 aattgtattt ttttctagtc aaagaagaaa aaaagtcaaa aaattcttaa gaaaaaagtg
    21481 ttttttcttt agaaaacgta ttcttagttt tacttctgag atggttttag taaaataaag
    21541 gaggaatgtt ttttgagacc cttagtggac aaaaaaattc gtgaagcagc gcaactcgtt
    21601 ttgggcgctt tattttttgc ttaaaaatca cttttacgtg agaaggagtt agattatggc
    21661 aaactatggc caaggagaat caagcggaaa gatcatattg atgggcgagc acgcggttgt
    21721 ttatggagaa ccagcgattg cctttccttt ctatgcaaca aaagtcaccg cattccttga
    21781 agagctggat gcaatggacg atcaactggt ttcttcctac tattcaggaa atttagccga
    21841 agctcctcat gcattaaaaa atatcaaaaa attattcatt cacttaaaaa aacagcatga
    21901 catccaaaaa aacttgcaac tgaccattga aagcacgatt cctgctgaac gtggaatggg
    21961 atcaagcgct gcagtcgcca cagcagtcac tcgtgctttt tatgattact tagcatttcc
    22021 tttgtctcgt gaaatactat tagaaaatgt ccagctttcg gaaaaaatcg cccatggtaa
    22081 tcctagtgga atcgatgcag ccgctactag cagcttgcag ccgatttatt ttacaaaagg
    22141 gcatcctttc gactactttt ctttgaacat cgatgctttt ttgattgtcg ctgatacagg
    22201 aatcaaagga caaacaagag aagccgtcaa agatgtcgct cacctctttg aaacccagcc
    22261 tcatgaaact ggacaaatga tccaaaaatt aggatacttg acgaagcaag caaaacaagc
    22321 aatcattgaa aattcaccag aaacgttggc acagacaatg gatgaatcac aatcacttct
    22381 ggaaaagctg acaatcagca atgattttct taatctattg atccaaacag caaaagatac
    22441 cggggcattg ggtgccaaat taactggcgg tggacgcggt ggctgtatga ttgcattagc
    22501 acaaacaaaa acaaaagccc aagaaatcag tcaagcactt gaagatgcgg gtgctgctga
    22561 aacttggata caaggattag gagtacatac ctatgtttaa aggcaaagca cgcgcatata
    22621 ccaacattgc tctaatcaaa tattggggta agaaaaatga agaactcatc cttccaatga
    22681 acaacagcct ttcgttaaca ttggatgcat tttatacaga gacagaagtc atcttttctg
    22741 atagctatat ggtagatgaa ttttatctag atggcacctt gcaagacgaa aaagcaacaa
    22801 aaaaagtcag tcagtttctt gacctttttc gtaaagaagc cggcctctca ctaaaagctt
    22861 cagtaatcag tcagaatttc gttcctaccg cagctggatt agcctcctct gccagcgggc
    22921 tagctgcttt agcaggcgct tgcaatactg ctcttaagct tggattagat gatctctctc
    22981 tttcaagatt tgctcgacgc gggtctggtt cagcttgccg aagtattttc ggtggtttcg
    23041 tcgaatggga aaaaggccat gacgacttaa gttcttacgc taagccagtc ccttccgatt
    23101 ctttcgaaga cgatttagca atggttttcg ttttgatcaa cgaccagaaa aaagaagtgt
    23161 ccagcagaaa tgggatgcgt cggacagtcg aaacatccag tttttatcaa ggctggttag
    23221 attccgttga aggggatcta tatcaattga aacaagcaat caaaacaaaa gatttccaac
    23281 ttctcggaga aacgatggaa agaaacggac taaaaatgca tggaacgact ttagctgccc
    23341 agcctccatt cacttactgg tctcctgaca gcttaaaagc gatggatgcg gtgcgacaat
    23401 tgagaaagca aggcattcca tgctacttta cgatggatgc tggccctaat gtcaaagttt
    23461 tagtggaaaa cagccattta tctgaggtac aagaaacatt taccaaatta tttagcaaag
    23521 aacaagtaat tactgcacat gctggtccag gaattgcgat tattgaataa agggagattt
    23581 ttatgattga agtatctgca ccaggcaaac tctatattgc cggagaatat gcagttgttg
    23641 aaacaggcca tccagcagtt atcgctgcag tcgatcaatt cgtgacagtc actgtagaat
    23701 ctgcacgaaa agtcggaagt atccaatctg cacaatatag tgggatgcct gtacgttgga
    23761 caagacgcaa cggagaatta gttttagata ttcgggaaaa tccttttcat tatatccttg
    23821 ctgctattcg cttgactgaa aagtatgcac aagaaaaaaa cattctttta tccttttatg
    23881 atctgaaagt aacaagtgag ttagacagtt caaatggccg gaaatatgga ttgggttcta
    23941 gcggtgccgt gaccgttgca acggtcaaag ccttgaatgt tttttacgcg ttgaatttat
    24001 ctcagttgga gattttcaag attgcagcac tagccaattt agcagttcaa gataatggtt
    24061 cctgcggcga catcgctgct agctgttatg gtggctggat cgctttctca accttcgacc
    24121 atccttggct ccaagaacaa gaaactcagc attctatcag tgagttactt gccctggatt
    24181 ggccaggtct atccattgag ccattgattg ctcctgaaga tttacgttta ttgattggtt
    24241 ggacgggtag ccctgcctct acttctgatt tggtcgatca agttcaccgt tcgagagaag
    24301 ataaaatggt ggcttatcag cttttcttaa aaaatagtac agaatgtgtc aacgaaatga
    24361 tcaaagggtt taaagaaaat aatgtaacgt tgatccaaca gatgattcga aaaaaccgac
    24421 aattactgca tgatttatct gcaatcactg gggtcgtcat cgaaacgcct gctttgaaca
    24481 aattgtgtaa tttagctgaa cagtatgaag gagccgcaaa atcttctggt gcaggtgggg
    24541 gcgattgcgg aatcgtaatt gttgaccaga aatctggcat tcttccttta atgagtgcat
    24601 gggaaaaagc agaaatcact ccactgccgt tacatgtcta tagcgatcaa agaaaggaaa
    24661 accgatgaat cgaaaagacg aacatgtttc actagctaaa gcctttcatg ataaacaaaa
    24721 aaacgaattt gacttcgtac ggatcatcca taaccctttg ccgcaaatag cagtttctga
    24781 tgtcgatcta agtacacagg cagttgggtt tacactaagc agcccatttt atatcaatgc
    24841 gatgactggc ggaagcgaaa aaacaaaaaa aatcaaccaa gatcttgcaa tcgtcgcaag
    24901 agaagcagat ttgatgattg ccactggttc agtcagcgct gccttgaaag tcccttcttt
    24961 agccgatact tatacgatta tgcgacaaga atatcctcac gggaaaatca ttgccaacat
    25021 cggtgcaggg acttctgtcg aaagagccaa agaagctatc cgattgtttc acgcagatgc
    25081 cttacagatt catttgaatg cacctcaaga gttggttatg cctgagggag accgtgattt
    25141 taccaattgg aaagtactta ttcaagaaac tcaaacagcc attgacgtcc ccctcatcgt
    25201 aaaagaagtc ggttttggca tgacaagaga aacgctcaat gatctagctg ctttaggagt
    25261 tcatacagtt gatatcagcg gtcgaagcgg aacaagcttc acccagatcg aaaacgcccg
    25321 tcgctccaag cgggaactga gttatttagc tgactggggg caatcgacag tctcttcatt
    25381 gcttgaagca aatgaagcag acacttctat ggaaatccta gcctccggag gtatccgcaa
    25441 tgcctatgac atatttaaag ccctttgtct cggcgcaaat gccgtgggta cttcaggaac
    25501 cgtactgacg catctgatga atcacggcgt agaagaaact attatactga tgaagcaatg
    25561 gcaagaagag cttcgtctgc tttatactat ggtcggcgca acgaataccg cggccctaca
    25621 ccaacaatct ctgatctttt ccggaccagt gaaggattgg tgcgaagcaa gagggatcga
    25681 cttagtgaag tatggaagac gcacagaaaa gagcgtggga caaaagtaaa gaacactttt
    25741 gtctcacgct ctaaacacgt acaaacggcg gaagtaaaag caactccttc ggaaataagc
    25801 tgaaattcac aaaaatttga aaagcaattt tcgtgaattc cttcttattt cttggagtta
    25861 aatgcttctg tcccagcctc ttttttcgaa taaatgatgt ttaatcagct gtccttcggt
    25921 attcgttcaa tccgctgatt tcacaacctc tccactaaac agtgtactaa agctctttta
    25981 aggttgtttt ccgatatagg caagtatgcc tccgtccaca tacaagacat ggccgttaac
    26041 gaaatcagat gcgctagaag ccaagaagac agatggtcca gccaaatcga ttggctcgcc
    26101 ccagcgtgct gctggcgtac gtccaacgat aaattgatcg aatggatgac gttcaccgtt
    26161 ttcttgtgtt tcgcgtaatg gagcagtttg cggtgtagca atataacctg gtccaattcc
    26221 attacattgg atattgtatt gaccgtattc agaagcgatg tttttcgtca gcatttttaa
    26281 tccgcctttt gccgcagcat aagcactgac cgtttcgcgt cctaattcac tcatcatgga
    26341 gcagatattg atgattttac cgccgccttt ttcgatcata tccggaataa ctgcttttga
    26401 aacgatgaat ggggcattta aatccacgtc gattacttgg cggaagtctg ccgcatccat
    26461 ttcgatcatc ggtgtccgtt tgatgatccc tgcattgttg actagaatat caactgaacc
    26521 tacatcttct ttgatctgtt gaaccatcgc ttgaaccgct tcttcatcgg tcacatcgca
    26581 gacataacct tttgcatcga tgcctgcttc tttataatgc gctatacctt catcaactga
    26641 ttcttgattc aagttattaa atacgatttt tgctcctgct gtagctaaag agcgtgcaat
    26701 ctcaaatcca ataccataga cagcaccggt gactaatgct acttttccat ctaaacgaaa
    26761 catatccata ctaaattcca tcattttctc tcccttatgc ttattttaat tgatccattt
    26821 gcaccatatc catgtctgta taggtgatat tttctccgca cattgcccag atgaatgagt
    26881 aattgcttgt acctacacct gaatggatcg accagcttgg cgaaataatg gcttgttcat
    26941 tgtctacaac taaatgtttt gtttcatctg gtttgcccat catgtggaac acacgtgtat
    27001 cttcattggc ataatcaaag tagacataag cttccatacg acgttcatgt gtatgacaag
    27061 gcatcgtatt ccaagcgctt cctggttcta aaatcgtata acccatttgt aattgacagc
    27121 tttcgcatac atttggatgg atgtattggt agattttacg ttcattcagt gtcaggcctt
    27181 caccagtctc cattggtttt acttggtcga tactgatttt tacatttgga tatttatgat
    27241 gagctggaac agaactaata tagaatttag ctgggttttc agcatcttga gaagaaaata
    27301 caacatgtcg tgtttctttt ccgatatagt atccgtcttg ttttttcatt tcttcttttt
    27361 gcccatcgat ctcgatgaat cctggtccac cgatattgat cacacctagt tcacgacgct
    27421 ctaagaagta atcaacacct agttctttgt ctaatgtgat ttctaattct ttttcagtag
    27481 gtgtcactcc accaaagatc attcgatcgt tgtgcgtata agtcaaactg attttccctg
    27541 gttcaaatac cttttctact aaaaattctt ttctcaattg ttccgttgaa taatgacgga
    27601 tatcttctgg actgtgtgta taacgtgttt ccatttcttg cattttcttc tactccttct
    27661 atcgaatgat ttttccagag ccgcatgcta aaaattgtgc gacttcttct tgactaaatt
    27721 ggttgcagtc cccatggatc gtatgcttca aggcagaagc tgccgtagca aaatcaatga
    27781 tttcttgcgg agacatattg gataaaatcc catggagcac gccacctgca aaagcatctc
    27841 cgccacctac tcgatccacg attggggtga ttctatggac ttgggattcc acatattcat
    27901 ggttcatcca caatgtcccc ttcaattcat tttcacttgc tgaatgaacg gtacgcttgg
    27961 tcgaataaaa cacctgaata ttcgggaact tttcctgcat tttttgataa taataaatta
    28021 gaggttgttc ttctgcttct ggcgctttct cgatacctaa caaataaaga gcatccattt
    28081 ttccagctga gcagatatct actaaaggta aaagctgatg gattacttcg ctggcttctt
    28141 gctgtgtcca taattttcca cgatagttga tatcgaagct gatcaggcag ccagcttttt
    28201 ttgcagcttc tatcacctgt ttcgtcattt tttgccattt tttagacaaa gctggggtga
    28261 ttcctgaaac atgaaatata tctattcctt ggaataactc ctctagcgac cattccaaat
    28321 gatccatttc agcaaaactt gatccggcac gatcatagat cactgaggcg gcgcgttcgc
    28381 cactaccagt ttccatataa taagtaccta gtcttgatcc accagataat acctgtttcg
    28441 tatctactcc ataacattgc aagtgctttt tgactgcttt tcccaatgcg ttatcaggta
    28501 cttttgtagc aaaaatcgct tcatgtccgt aattagctaa agaaattgct acgtttgctt
    28561 cccctccgcc ataatgggca gcgaatcgtt cacttgaatc gatacgggtt ccctgctctg
    28621 tggacaatcg caacatgatt tctccgagtg tcactacttt tcccataagc agctacctct
    28681 tgatttcttt catttttgct gcatactgtt gcgcgacttc cgttactttg tcaaaatctc
    28741 ctgtagctgc gggtgctaaa aggttcccac ctactccgac tgttacgaca ccagcatcga
    28801 accactcttt catattgtcc agactcacac cgccagttgg catgatattg acttgaggca
    28861 tcggcgcctt gaaggcagaa atgatacttg gtccataagc gcttccagga aatagtttga
    28921 cgatatctac cccgcttttc agcgctgttt tgatctcggt aatagtcata catcctggta
    28981 agtaaggaat ttgatacaga ttgcacatct ctgccgtttc ttgatcaaag gaagggctaa
    29041 caatatactc tgcgccagct aagatagcca agcgagctgt gacagcgtct aatactgtgc
    29101 ctgcgccaat cactatttct ggttggtctt tgtagaatga caaaagttct ttgattactt
    29161 gatctgcttg agggactgta aaagtcaact caataccagt tagaccacct ttgatgattg
    29221 cgtggcaagc attcaaagct tcttctttgc tttttccgcg taccaccgca atcactccgg
    29281 cgtttttcaa tcgagataaa atatcaattt ttttcatctt ctccacctat ttcctattta
    29341 gaaattttat tttataactt ggaaatctat acccatatta tacggcagaa aagaaaaatt
    29401 gcaaacgctt ttttcattat ttttgaattc tttgctcgat ttcgcttttt gtttgcttga
    29461 gggcactgat tatttgttct tttgtctcat ctgttaaacg atatttcggc atacttacac
    29521 taaatgcacc aatgatttgt tcgtttttca cgattgaggc tcctatacaa aaaatatcac
    29581 gttcgatttc ttcatcgtca aatgcgatgt gggtttcttt taccgttttg atttcctttt
    29641 tcaaccgtaa tgcattggtg atcgtatatt ctgtgtaagg gatcaacgga tgcttttcaa
    29701 gataatcagt gtactgctct tcactgaatt ctgctaatac ggctttgccc atcgcagaat
    29761 tgtataatgg tcgggtgatt ccgatttttg aagacatgcg gatcgtttga tgttttggat
    29821 ctagtttgtt cacgtacaag atctcattgt tgtccaatac ccctaagtgg atagtctcat
    29881 cgatcttttg ctgcagctgt tctaaaaacg gtaaagtcaa ctctaccaaa tcgatttgtt
    29941 caatgttttt attcgcgtag cgaataagct ttgcacctaa acgatagttt ttctctgaat
    30001 ttttatttac gtagccgatc atcaacaaag tatctagtat tttcaagact gtagaagagg
    30061 tcattcccac accctttgcg atcgtctgca aagagacatc ttggttttcc gctaaatagt
    30121 ctagtatttt agaggctttg atcaatacag ttccataagg ctttttattt tccatataat
    30181 attctcctat ttctttataa gaaaaattat agcggaaata ccatcaattt aaaagagata
    30241 tccagatata aactaccatt tctctttaca ggcaatttaa tgttgaagat ccgcttcgtt
    30301 tttacatcaa actttgatat aaagcaatga tttcttctac tgtcgtttct cttggatttc
    30361 ctggtgtgca tacatctcgc aaagcatctt ctgcaattgc tggaatatct gcttctttta
    30421 cacctaattc agaaatggtt tctgggattc caaccgcttt gctcaaccga tcgatctcac
    30481 cacaagccgc ttctcttaca tcttctaaag acatttcagc tgctccttta atccctagaa
    30541 caagagcgat ctcacggtac ttttctccag tatattcttt gttatatttc atgacagttg
    30601 gcaaaagtgc tgcacacgct acgccatgag gaatgttgta ccatgcactt aacgggtgag
    30661 ccatcccgtg taccagtcct aaaccgacat ttgagaagcc cattcccgca atatactgtc
    30721 ctaatgccat tttttctcgc ccattttgat cgccatctac agaagcttcc aaagaatggc
    30781 cgatgatttc gattgctttt aaatgcagca tatcagtcat ttcccaagcg ccttttgtga
    30841 taaacccttc aatagcatgc gtcatagcat ccatacctgt tgatgcagcc aattttttcg
    30901 gcattcccat catcatatcg ctgtcgatga acgcgacgat tgggatatca tgaggatcaa
    30961 cacaaacaaa ttttcggtga tttacttcat ctgtgatgac gtagttgatc gttacttctg
    31021 ctgctgttcc agaagtcgtt ggtatagcta atataggtaa acatggattt ttcgtatcag
    31081 caacaccttc taaactaatg acgtctgaaa actctggatt cgtcaaaata atcccaatgg
    31141 cttttgccgt atcgattggc gaacctccac cgattgcgat caaacagtct gcctctgcct
    31201 ctttaacaaa agccactcca tcttttacat tttggatcgt tggattaggt acgatcccgt
    31261 cgtataaagc ataagcgatt gctgctttat ctagaagttc tgttactttt gttgctatac
    31321 cgtgctcgat caatcctttg tcagtaatga cagctgcttt gccgaatcct cgctgctgaa
    31381 attcagggac gatcgactca atcgctcctt ttccaaaata cgatgtttca tttaaaatca
    31441 ttcgattact cattttctat tcctcctaat ttgtattatt tttcacaata aaaacgaaag
    31501 aaccccaagc aaccggttgc ccagttgctt gttgctacag ttcaaagact ggcatcaatt
    31561 cccaactgac tggagaggca tctggatttg tctccatgat atcttccatg tacttccacc
    31621 atttttgatt gatagatgta tttgccattt tttcccataa cttttcatct tcgatcacta
    31681 agtaaccaaa taacaaactt gtactcggat caagatagat cgaatagtcg atgaccccgt
    31741 gttcacgaag tgctgtacgc atttctggcc aaagttcatc atgtcttttt ttgtattctt
    31801 cttgtttttc tgggtaaact ttcatgcaaa aagctttttt caccatgtca gacacctgct
    31861 ttcgtatgaa cttcctccaa ataacctgct tttggagtga caccaaacgc atctgctaaa
    31921 tgccacaggt tttcgtccgt gattgtttgc aagattggtc cttgcgcttt gacgtacgta
    31981 tagacttctg ctgccttttc tgctgtctcg atcaaaccaa agacttcatc catgtctttt
    32041 cctgacccgt agattccatg ttgtggccat aaaaccaatc gagtttcttt cattttttca
    32101 gctgttgctt ccccaatctc atttgttccc ggaaccatcc acggaatgat ggaaattcct
    32161 tctggaaaaa caactaagca ttccgtacac atttgccata gcgtacgact aaaggaacgt
    32221 tcgtctaatt catgcgtaaa actcatcgct aatagatgac tagcatggca atgcataacg
    32281 atccgattat ctgggtctac ttctaaacgg gcgatatgac tcatgaaatg acttggcaac
    32341 tcgcttgtag gaaggcattc gttttccagc ccccacaaaa gttccagtgt tttgccatct
    32401 tcagcaactc gaacgattcc aagattctcg gcaggttcgc tcgctacatt tttaaaatat
    32461 cttcctgaac cagttacaat aaaatagcat cctgctaaac ggctggcatc aaaaatcatc
    32521 gagatgcggc ggatcacttt ttcaggatcc aagaacggtg tgatctcttc ttcttttagt
    32581 aaataagaaa tattcccgcc atttctttca tcccatccca agcgataaag attcgacgtc
    32641 gtttcgatca tttctttcac ataaggcgct tgtaaaatat ctattttttt caatatagcc
    32701 acactcctat tttcttaatt ccaacacgtc tttttgatat tcctggactt cttccagcca
    32761 atccaaacca actggaactt gatttttgta acagaaataa ttccatacat cggcatacgg
    32821 gaaatctttc aattcttccg tataagccat tcttgaagta aagtcaaaag ccagctccat
    32881 ttctttcaat cgctcaactg gttccagcat agctttcagc agtgcttttt gtgtatttct
    32941 cgtaccgatg acccaagcag cgatacggtt gatcgttgca tcaaagaaat ccagaccgat
    33001 atttgttttt tccaacaatt cgttacggac taattcttta gcgatttcct gcaattcatc
    33061 atccatcaaa accacatggt ctgaatccca acgcactgga cgggaaacat gcaacatgat
    33121 tcctttgctg aataaagcta atgaagataa tttattggaa atcacttctg ttggatgaaa
    33181 atgtccagca tctagacaaa caagtttgtc acgagtcaac ccgtaaccca tataaaattc
    33241 atgggaaccg actgtataag cttcagcgcc taaaccaaat agtttgcttt ctactgcatc
    33301 ttgtacatac cgttcatcta actcttctgt aaaaatttca tcgagtgatt ccattaaacg
    33361 ttcccgtggg gtaaaacgat caatcggatt atctttcatc ccatcaggta cccaaaagtt
    33421 attgatactt gtttgcccag tttctttccc aaagtactcc gcgattttgc ggcttctttt
    33481 cccgtgttcg atccagtatt cacgaacttc tttatctgga tgagccaatg taaagccgtc
    33541 tttcatcatt ggatgcgaaa agaaagtcgg attgaagtca agaccgattt cttctttttt
    33601 tgcccactct acccattttt caaaatgttt cggctcaatt tcatttagat cgacagattc
    33661 ttctgtatcc aaatagatag catgaaggtt caatttgtgt ttgcccggta tcaacgaata
    33721 agccttctcc agatcttgtc taagctcttc aggtgttcgt gcggctcctg gaaaattccc
    33781 tgtcgccatg attccaccgg tcaattcgcc atccggtgtc aagaaacctt tgacatcatc
    33841 tccttgccag caatgcatag aaattttgat gtcctgtatc ttttctagta cagcatctgt
    33901 atctacaccg atagttgcat atttttcttt tgcttgttcg taatttcttt caatcgtgtt
    33961 catacgatta ctcctttgat tttattcacg taccattcca tcgtgttttg cttattttca
    34021 tctgggtaaa aagtttcaca tggaaacgac tgtttgatta catttcttgc ttgttctata
    34081 ttcgaaaact gacctgtact gagcatttgc atggcaacat tcccgattgc tgtggcttca
    34141 ctaggtcctg cttctacaat gattccggca taatctgctg tcaattgatt cagtagacga
    34201 ttattcgatc cgccccctac gatgtgcagc ttttccaatt tcgttccagt taagtgttct
    34261 actttgatca gctcttgtgc gtaacaaaga gcaagattat catacacaca acgagcgata
    34321 gctcccgatg agacagggac tggctgctct gtttcaaaac agtattgctg aatctcttct
    34381 atcatatttg ggggattcaa aaatcgaggg tcattgattt ccatcacttg ctgcagcggt
    34441 ttttcttttg cagcaagctc tgccaattcc gcataactgt actcgtactc ttgcattcgg
    34501 gcaacttcct ggatcaacca catccccatg atgtttttca aaaaacgcac cgtgttatgg
    34561 gcaccccatt cattcgtata attttcagaa aaagcattgc gagaaacatt ggcgatcttt
    34621 gtttcaatcc ctaaaagcga ccatgtccca ctgctgatat aaccccaacg ttctccttga
    34681 cctggcgttc ctaatacagc tgacgccgta tcatgacttg ctactgtgat aaaggtcaca
    34741 tctggcaaat catattttga aaattgttct ttttgcagat ctcctagtat cgttcccggc
    34801 tctgtcaatg gtggaaataa ttccttcgag acaccgattt tttcaagaag ttctatttcc
    34861 cattcttttg tccctgcatt cagcagctgc atagtagatg cattagtttt ctccgtcacc
    34921 atgttaccag taaagacata ccctaaataa tcaggtatca gtaacaattt atgtgctttt
    34981 gccaaatttt ctttttcttc agcaaacaat tggaacaagg tattgaaagg ctggatctga
    35041 atccctgtct gctcatacaa cttatctaga gaatagattt cagaaaactg ttctactgct
    35101 ccattggtac gcttgtcccg ataagcaatc ggatcattta tccgatcccc ttgttcatca
    35161 atcagacagt aatcgactgc ccatgtatcg atccccgcaa aaaggcgatc aatccctgtc
    35221 tgctttaatt tttcaaatcc aatcaaaatc tctttgataa gataatcgat cttccagcga
    35281 tcgtgaccgt ctttgttccg aaagccgttt ttgaaccggt gtacttcttc caagatcaaa
    35341 gaaccttttg catccctcgt acttttcatt agtcgtccgc ttgatgcacc gatatcgatg
    35401 gcaacaaatg ttggattcat ttctccacct cttttacttt attgttgatt agcactttat
    35461 taataaagcg ttatcattta ttgacaatgc tatcataata cagaatctta tattttgctt
    35521 cacactattt tggtatataa ttgcattatc ttgacaagtt gggagtgttt acatggatta
    35581 ctgggaaaaa gccaaccact catggtcgga agactcaaaa agagtgatct tgacgcctac
    35641 acaaagaagc aggaatttat ttttttatat ccaagaaatc ggttacttca aagcaccaat
    35701 cccttactat actgaacgag cacatttacc ttcttacttg ataaagttta ctttgtctgg
    35761 caaaggttcg cttacatata atgataatca gcaacagcta aaagctggag acttattttt
    35821 tattgactgc caagaatatc agcgttatca aacgatcagc gaagaaccat gggaaatgga
    35881 ttggatccat ttgaatggtc agaacgtcca atcattttat caggagttca caaaagacgg
    35941 ccataacacc ttccattcaa caggagatcc atcagaaaac aagatccacc atttgatcac
    36001 agaattgatc cacatgcaac aaggcacgaa tgcccgtacc gatttccagt cctctgtagc
    36061 tatccacgag ttgctgaatg aactgatttt gcaaaagtat cagctagatt tcgaagaaga
    36121 ggatattccc gcctacgttt acaaactgaa gcaatactta gacgaacatt tcaaagaaac
    36181 gattacactt gatcaattag aacaacaatt cttattgaat aaatatcagc tgagcaaaga
    36241 tttttccaaa tttatcggtt ctccaccaat cgattatttg atcagcaaaa aactttctta
    36301 cgctaaagac cttcttcggt atacagaatt tacggttcga atgatttccg aagaaatagg
    36361 gattgagaat tttgcttact ttagccgcct gttcaaagca aaaacaggac tatcgccgag
    36421 agactttcgt aaattcagct gattcatata atatgtacgt cattttttca aaatattttg
    36481 aaatatatag ataaaaataa aataaccgca gtttctgctt ctatgtaaac aacagcagct
    36541 actgcggtta ttttttagtg atcattctca ttgttcatct tcaaaatgtt cataatgaac
    36601 caactgtttc tgtgtatcac gtttcaaaaa ttctttaggc gttatgccga actctctctt
    36661 gaatgctttc agaaaatgcg gataagaaga aaaaccactt ttactatata cagcactaga
    36721 gctgcggtat tctcgcaaca agtgcttggc atagatcagc tttttcttca ttacatactg
    36781 atgaaacgta aatccagtgt actttttaaa ttctcgtgtg atatggtatt tgctgacaaa
    36841 aaactgcttt tccatttcct ctaaagaaag cggatgagat aaattttttg agatatattg
    36901 taacatttgt tccacacgct cattaggaac tacagcattc tgttcttgcg cactctcttc
    36961 tttttgtaca acttgattga gaaaaatcaa gtattctacc agagcttgtt catattttat
    37021 gtcttcaccg taaatttttt tatcaggagg atggtcgata aatgataatt tttgcttcaa
    37081 tacttcctgt tctatttgca atacacgact cctcttctct cccattggtt gaaaacaagc
    37141 cgatagatta gaccatggcg tggaacgact ttctagaaaa gaaggggtga caaagatata
    37201 gacacgctcg aatacatctg aagattggcg gacgatacga tgcaaatcat tggaatggat
    37261 gagcagcaca cttcctgctt tgatcgtaaa ttgtcgtccg ttcaaataaa aagtcgcttc
    37321 tccctttaat gtagcatgga tctcatagaa atcatgatga tgatagatcg ttttattttg
    37381 gctgacgaca tctttgacat gatagatttc atactgatca gaaaccatcg tattgacttg
    37441 ttcaccgctg attttctttt ggagcatgtg ccgcacctcc ttttgttcac ttatgcacaa
    37501 ttttattata ccacgttttt tgtaatcaaa aagagctgct tttatatact cttataaaaa
//
LOCUS       DAZKLA010000013        37079 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000013 DAZKLA010000000
VERSION     DAZKLA010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.013"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..384)
                     /locus_tag="ACG85N_000550"
     CDS             complement(<1..384)
                     /locus_tag="ACG85N_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223346.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKI"
     gene            complement(569..967)
                     /locus_tag="ACG85N_000551"
     CDS             complement(569..967)
                     /locus_tag="ACG85N_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFQ0223347.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(964..1737)
                     /locus_tag="ACG85N_000552"
     CDS             complement(964..1737)
                     /locus_tag="ACG85N_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0223348.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1734..2417)
                     /locus_tag="ACG85N_000553"
     CDS             complement(1734..2417)
                     /locus_tag="ACG85N_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0223349.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2584..3471)
                     /locus_tag="ACG85N_000554"
     CDS             complement(2584..3471)
                     /locus_tag="ACG85N_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0223350.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3468..4337)
                     /locus_tag="ACG85N_000555"
     CDS             complement(3468..4337)
                     /locus_tag="ACG85N_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0223351.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4474..5760)
                     /locus_tag="ACG85N_000556"
     CDS             complement(4474..5760)
                     /locus_tag="ACG85N_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0223352.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            6033..6611
                     /locus_tag="ACG85N_000557"
     CDS             6033..6611
                     /locus_tag="ACG85N_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0223353.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6611..7540
                     /locus_tag="ACG85N_000558"
     CDS             6611..7540
                     /locus_tag="ACG85N_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0223354.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7537..9273
                     /locus_tag="ACG85N_000559"
     CDS             7537..9273
                     /locus_tag="ACG85N_000559"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0223355.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(9336..10172)
                     /gene="pheA"
                     /locus_tag="ACG85N_000560"
     CDS             complement(9336..10172)
                     /gene="pheA"
                     /locus_tag="ACG85N_000560"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFQ0223356.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(10169..10678)
                     /locus_tag="ACG85N_000561"
     CDS             complement(10169..10678)
                     /locus_tag="ACG85N_000561"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFQ0223357.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10671..11966)
                     /gene="aroA"
                     /locus_tag="ACG85N_000562"
     CDS             complement(10671..11966)
                     /gene="aroA"
                     /locus_tag="ACG85N_000562"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0223358.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11979..13070)
                     /locus_tag="ACG85N_000563"
     CDS             complement(11979..13070)
                     /locus_tag="ACG85N_000563"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFQ0223359.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(13087..14253)
                     /gene="aroC"
                     /locus_tag="ACG85N_000564"
     CDS             complement(13087..14253)
                     /gene="aroC"
                     /locus_tag="ACG85N_000564"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFQ0223360.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(14255..15319)
                     /gene="aroB"
                     /locus_tag="ACG85N_000565"
     CDS             complement(14255..15319)
                     /gene="aroB"
                     /locus_tag="ACG85N_000565"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFQ0223361.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(15352..16374)
                     /gene="aroF"
                     /locus_tag="ACG85N_000566"
     CDS             complement(15352..16374)
                     /gene="aroF"
                     /locus_tag="ACG85N_000566"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFQ0223362.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(17033..18007)
                     /locus_tag="ACG85N_000567"
     CDS             complement(17033..18007)
                     /locus_tag="ACG85N_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFQ0223363.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17988..19871)
                     /locus_tag="ACG85N_000568"
     CDS             complement(17988..19871)
                     /locus_tag="ACG85N_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFQ0223364.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19889..22114)
                     /locus_tag="ACG85N_000569"
     CDS             complement(19889..22114)
                     /locus_tag="ACG85N_000569"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0223365.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            22229..23263
                     /locus_tag="ACG85N_000570"
     CDS             22229..23263
                     /locus_tag="ACG85N_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0223366.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(23330..24343)
                     /locus_tag="ACG85N_000571"
     CDS             complement(23330..24343)
                     /locus_tag="ACG85N_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0223367.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24421..24609)
                     /locus_tag="ACG85N_000572"
     CDS             complement(24421..24609)
                     /locus_tag="ACG85N_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFQ0223368.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(25049..25177)
                     /locus_tag="ACG85N_000573"
     CDS             complement(25049..25177)
                     /locus_tag="ACG85N_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0223369.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(25180..26553)
                     /gene="rlmD"
                     /locus_tag="ACG85N_000574"
     CDS             complement(25180..26553)
                     /gene="rlmD"
                     /locus_tag="ACG85N_000574"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0223370.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26664..27719)
                     /locus_tag="ACG85N_000575"
     CDS             complement(26664..27719)
                     /locus_tag="ACG85N_000575"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0223371.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27729..29159)
                     /gene="gatB"
                     /locus_tag="ACG85N_000576"
     CDS             complement(27729..29159)
                     /gene="gatB"
                     /locus_tag="ACG85N_000576"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFQ0223372.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(29156..30628)
                     /gene="gatA"
                     /locus_tag="ACG85N_000577"
     CDS             complement(29156..30628)
                     /gene="gatA"
                     /locus_tag="ACG85N_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFQ0223373.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30628..30933)
                     /gene="gatC"
                     /locus_tag="ACG85N_000578"
     CDS             complement(30628..30933)
                     /gene="gatC"
                     /locus_tag="ACG85N_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFQ0223374.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(31039..33075)
                     /gene="ligA"
                     /locus_tag="ACG85N_000579"
     CDS             complement(31039..33075)
                     /gene="ligA"
                     /locus_tag="ACG85N_000579"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFQ0223375.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(33092..35326)
                     /gene="pcrA"
                     /locus_tag="ACG85N_000580"
     CDS             complement(33092..35326)
                     /gene="pcrA"
                     /locus_tag="ACG85N_000580"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFQ0223376.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            35481..37040
                     /locus_tag="ACG85N_000581"
     CDS             35481..37040
                     /locus_tag="ACG85N_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFQ0223377.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT        10240 a         8053 c         6687 g        12099 t
ORIGIN      
        1 aatcttttgc tgacgtctaa aaatagaaaa agaattttcg tcatctattt gataacgcat
       61 ttgtttttgc agttctgctg cagtcagatg gatcttccca tcgtttgtat cgatcattaa
      121 accttctgga aacagttcaa ccactgctcg atcccaagat tcaagagaaa gctcgatata
      181 atcttttact ggtaattcca attctacctt aaaaaagtgc aaaacagccg acagcccttc
      241 tgcttgatag atttctccta atgttagccc ctctagatgg aactctggtg aaaaatgcag
      301 caatgtttta ttttcctgca attgaacata tagaaatgta gatcgctcaa tcagagtatc
      361 ttttaaaaca actaaaaaat tcatttttct cccccttttt tcatcatttt taaaatagca
      421 tattttgaaa agaaagtctg atgcttagag tagaatttca taaaaattta ctatttcata
      481 agtattcacg agaaaaatac aaacaaaaaa gcggaaataa cgctcatccg ccttcttgtt
      541 tgtgtttatt tcctcttcaa acgcttgtct atgattcaga cttttctcta tcgttatttt
      601 catctaaaag ttcttgcatt ccgataattt ctcgatattg atgaatctta gtgataccaa
      661 tgactttgtc tacatcaata ctaaaacaaa taccattacc tggaatatcg atgttgatgc
      721 cttgaccaat cgcatccatt actttattcg tcaaactatc gggtactaat gtcaacacaa
      781 tatctttttc tggcgaaatg tccaaaccta aaaatttccc tgaatcatgt acaccagaac
      841 ctcttccatg taagatcgtt ccgcctgctg cacctgcttc ttttgaaaag tcgataacat
      901 cttcgcccat tcctccgtca acgatcgtca caatcatgtg aagattcaat gctacaagat
      961 ctttcatttg ctttccccca atttttgcaa tctttctttt tcgattctgt ctttttcatc
     1021 ttttcgttta taaatatatc ctagtactaa agtggataat attggtgtca atgcaatcat
     1081 tgcaaccaca ccaaacccgt caatgatcgg atctcttcct tcaatttgtg cagcgattgc
     1141 cacgctcatt gataaaataa atgtcgaaca catcgggccc gttgctactc caccgttatc
     1201 aaaagccatt gctaaaaaga tcttgtccac ttttcgtcct aaaataaatg ctagcccgta
     1261 tcctgggatc aagaaataat aaagagagaa tccgacgagc aaacgccaca tagacaatcc
     1321 tactgctgcg ccgatcccaa ttgaaattac catcagcatg attttcgatc ggattctccc
     1381 ctcgctcaat tcttcaacct gtttgatcat tacatgaatc gccggctcag caaaccctac
     1441 taagaacccc attaaaaagc caaggggaac cagtatataa ttgtaaggca atcccgcaat
     1501 agcactgcct aaatgctgtc ctaccggtac ataagccata ttgactccgt gcaggaaaag
     1561 gatcaaccca atagtcgtta tcaagaaacc tttcataatg tcaaagaaac gctttttgcg
     1621 catacgaaaa ctaatgatat tcattaaaac aaataggatt acgataggtg ccactgccaa
     1681 aatgacttcc atggtaacga tttcgattcc atctatcact tgctgccaga aactcattgg
     1741 aagatcaccc ccaaaatcat cacggcaaga atcggcccta acgaagcaat accgaccatt
     1801 ccaaaactgt cattttcatt tttcttttgt ttgatcatac tagtcatccc tccggcaagg
     1861 gccaagataa atggaacagt tactggacca gtagtcactc cccccgaatc aaaagcaatc
     1921 ggcataaatt cggaactggt gaaaaatgag gcgatcaaaa cgcctatgta gcctatagcc
     1981 atcaattgat aataggatag cttgaatacg acccgaagca gcgcaaaggc tagaaatact
     2041 ccagtgccga cgctgacgat cccaatcagc aacactctgc caatctctcc gtcagatata
     2101 tcatatactt gttgtcccaa aacttgcacg gaaggttcag caatcgtgat cacgatcccg
     2161 atagcaaatc ccaaactgac caaaacggtc aaacttttct ttttgatcat atatttccct
     2221 actagatctc cgacttccat catagaataa tctgctccaa acaaaaagag cgtcattccg
     2281 atcatcatga ttgccgcacc aaccagaaat gaagcgaggt cttctccttc taacggtgca
     2341 aaaacaaacg ttaaaatgac aatcaggaca gtcatcggca atatagcagc aatcacttct
     2401 ttaaaatttt ttagcatgta tcctcctcct ttactcttta agtttacctt aaaatgaaag
     2461 agttttcgag cacaaactta aaacgttatc atttaatttt tctcaacata aaataacaca
     2521 cgtcccaaaa gtatcttttt acttggaaaa atgactttga aggaacgcgc ttattgtgtt
     2581 tttttatttt acagagccga tcattccttg aacgaattgc ttttgcaaaa caaaaaatac
     2641 gatggcagtt ggtaaagtag cgatgactaa tccagccatg atcattccat agtctggtgt
     2701 ataggaagca cccatagcag ataaaacaag cggaaccgtc cgtttatcag gagattgcaa
     2761 agctactaag ggccataaat aattgttcca gctactcatg aatgtgatga ttgcagccgc
     2821 ggcataggta tttttagccg tcggcatata cactctaaaa aaaatactta gttcttttag
     2881 accgtctaat cgagcggctt ctactagatc ttttggaaaa gatttaacat tttgtcgaaa
     2941 gaagaagatc aaaaaagcag tgctaacaga tggcaggatg acaaccaaga aactgttgat
     3001 tcccaaaacc gttccgttca attgtgaaaa cattcgatat aaagggatca tcaatgccgc
     3061 aaaaggaacc atcatcgata gcagtagaat attgaaaatt aggtcttttc gtttactttg
     3121 ataaatctca aatccatatc ctgccattga cgaaatcagc aatgcaaaca cagtggtcac
     3181 caaagcgatc atcgctgaat tccaaaggga acgagtaaaa ttcagctcat tggaaaacaa
     3241 attttgaaaa ttgatgatca agttactgcc aatctttatt tttcccgatg tgatatccac
     3301 gggtgtattc gtcattccca aaaccatcca gacaaaaggg aaaatggaaa taaatgatag
     3361 gatgcttaat gctgtatact ttaatactga cccaattgtc gaacgaagtt ctaatgcttg
     3421 ttttttgacg ttggggcgtt cgattgtttc tttttctacc atatttttca tcgactttct
     3481 cctcctactt tcgtttggat tatcgacaat atcacaataa aggaaacaat aacaaaggaa
     3541 actgctgccg catatccaaa attaggcgtg aatttataac tcaaattata gatgtattga
     3601 gatagggtgg tcgttgcatt tgctggtccc ccgccagtaa tattttgtac ttcatcaaaa
     3661 agctgcaatg tcccgatcgt cgaagtaatg gaagtaaaca aaatgattgg ttttaaattt
     3721 gggatcgtca ctttgataaa ctgctgccat ttagaagcac cgtcaataga tgctgcttca
     3781 taaatctccg aatcaatatt ttgcatgcct gaaaggaaaa aaatcatatt gtatccagtc
     3841 caacgccaag tgatcgaaag aatgatcaga actctcgccc aaattggatg agtcaaccag
     3901 ccaatcggtg tgtcaatcat accaatgccc aacaacaagt tgtttacaag gccagattga
     3961 gaaaatagac ttttcatgat cagagaataa ctgaccatcg aagtgataca tggaagaaag
     4021 atagcggttc gaaacaaccc tttatatttc aactttttat catttaaaag gttggaaata
     4081 aaaagtgcta gaaacaacat gatcggtact tgtacaagta aatacaggaa tgtattaaaa
     4141 aaagcttttt taaaagttgc atctgtcaac atccgctgat aattacctaa cccgttaaat
     4201 gtcatattga cagggggacc actttgaaaa gacgtgatca aagccaaaac catcggaata
     4261 aaaaccatga ttgatatcaa aatgactggc aagattaaaa aagcatgccc gtttttgaat
     4321 aaatctttct gcttcattct tttccctcca attctatcat tgattatcta catgaaaaga
     4381 caagaaagac aatatctgta aggcagacgt gatctttttt gtcttttcgt ttgtctgatg
     4441 aaaaagcgaa gaaattcact tttttaccac cgatcaatta gccaattgtg cttctacttg
     4501 tttttgtgta tctgctagta ccttgtctgt ctcttctcca ttgatgattc gatgaagagc
     4561 ttcagctacg actgcttcaa ttgcataggt ctcatgtccg taattcactt ctggtatttc
     4621 agatgtccat gttgaaaaat cgtcaaagat cttttgacca ctgtaaaatt cagaagcttc
     4681 ttgataagct tcttgttcat tcgcgctaag catcgtagat actagtccga tttctttcgc
     4741 aagagtgccc atcagttctt cattcgaagc aaacgttgct tctaggaaat ctttcgctga
     4801 atcttctcca gctactcctt tgatcacata ccagccagcg ccgccaaggt tagaagcttg
     4861 cgctttttgc agattttctg gaagtgcagg tattggtgca atcttccatt ttccagattg
     4921 gttttctgct ccttggatcg ttgaagaata ccaagcgcca gttgggctag aagctactgc
     4981 tcctgattgt atcgcagtta ctccagcatt ccagtcagag gtttgttcga ctagatcttc
     5041 tttcaaaaga gtggcaaaca attcaagacc atactttaaa gatgcattgt cttggatcgt
     5101 gactgtctcc ccatcttcag atgtatacca ttctcctgcc tgttgcataa tcatccttac
     5161 tcgaccaagg tcagaaggat tgacttcagc gattttcttt cccgtctttt ctttgacatc
     5221 tcgcgctact tgaatatact cgtcccactt cagattctcc atatcctctt ctgtataacc
     5281 tgcttctgcc atcagatccg tacgatagaa gtttgctgtt acgcccgaat cgaaaggaac
     5341 accgtatatt ttatctccta ctttgtttac cgcaaattta tacggtgcaa aatgatcttc
     5401 ctttacaata gcagacaaat tagcgaatgc atctggataa gaagtcaaat agccttgtat
     5461 cctataatct tcaatcaaga cgatattggg caatccttct gtattcccgc tcgctaaagc
     5521 ggtgttcagt ttctgaacga tatcatcctg agacatcgta acaacttcta tctctgtttc
     5581 ttcgttctca taaacttttt ttgcttcatt cactgctttg atattgaatg tctcatccca
     5641 cgcccagaca gtgacttttt cttctttgtc tgctgtacta ctagcacttc cgcatcctgt
     5701 taagaaacct gatgctgccg ctaaacaaac gactccagca attaactttc tgatgttcat
     5761 ttttatccgc tccttttcca tccattagtt gtttacgttt gctacattca tcataccaaa
     5821 atttgaaaac ggtttcttta ttattcagat acttcaaaaa tttctcctaa aataataaaa
     5881 aaatacaaat caactatgac aaaaggaata atcttagcat ctagaccaaa gatcgaaatg
     5941 gtgaggtttc tttaaaataa aaatatcctt gcattttctc ctagaaataa gtatttttaa
     6001 attacaagac tattcttaag gcgggtaaaa aaatgccaat acgcgtaatt cttatcgatg
     6061 atgaacagac gattttagat ggaatgaaac agttgatcaa ttggtctgag cacgggtttc
     6121 aattaatagg gactttttcc agtccgctga aagcgcttga tttttctttg gtacatgcaa
     6181 tcgatatcgt ggtcacagat gtaatgatgc ctgaattaaa tggaatcgaa ttatcgcggc
     6241 tattaaaaca gcaacaacct gaaacacaga tccttatcct ctctagctat gatgaatttg
     6301 atatggttaa agattctttt aaagaaggcg tcgctgatta tcttttaaag cctcgattga
     6361 gtcctgattt ttttctaaat tccttaactg ccgtttcaca aaaaatcaaa aaaaagccca
     6421 acaaaagccc aatcaccaat cgttatgagc agatctgcga gaatctttca cagcagattg
     6481 ctggacaaac tcgtccattg cttgaccctc ttctttttga acacaccttg tttttctgtt
     6541 atataccgaa aaacggccat tcagttcaaa acaaggctct aaacaaaaac agtcgatcac
     6601 ccattcattg atggatagcg acgattcgtt tactatttat cctgtttcta ccttttcgga
     6661 agaatccggc tatgtaatca atactgattc caaagataca cttaggaagt ttgttcaaaa
     6721 gtttcgtcaa gaagtttttc caggtgattt ttttgttgtt tctgattctt ttgcaatcca
     6781 agatatcaat gaaatattcc aagagatcag aaattgcacc aaaggacaac gtttctatca
     6841 taagggaaag aaccttgctt atcagcatga acttcttccg ttattaacca accacgagca
     6901 gcaagcaagt agttatttaa caaaaatcgt tcataaagac tacgctttag ctgtcaaaga
     6961 acttgagaat ttcttcacga cgatattgga acagcctatt gatccagctt atttgaaaca
     7021 tgaggcaatc aatcgtttct atgcattact caacgctttg gctgacgagc atgttgggaa
     7081 tgaagaactt tctttgctca agatcgccct tccttcttta ttagccgatg ctgagtattt
     7141 cgaagatttt tctattcttg tcaaagaaac acttgagaaa ctcacccact tttctgcaaa
     7201 tgcaaaaaaa gacaatttcg atatcttgac tttgatctac gagttcgtcc aggaaaatta
     7261 tgagaaagaa atcaatttac aacttttatc cgaaaaatat catttttcat atagctatct
     7321 ctcagccatc tttactgaaa aatttggtat caacttttca aaatatctaa aaaaagtccg
     7381 tatcagtaaa gccaagctct ttttgacgga aacacggctt actttgacgg aaatctgtga
     7441 gaaagtcggc tataccgaac tgggttattt ctccagagtt tttaaagaag agacaggaat
     7501 gacaccttct caataccgta aaggaacttt tgtaaaatga gaaaaaaaat caaagagcat
     7561 gaactctttg ccaaacttct gttcattttg ttcatcggtc tttttctgca agccatcgtc
     7621 atttctttgt ttatttatca tcgatcaagg gatgcctata tccagctgtt caatcaatcg
     7681 aatgatgtgg tactaaaaaa gattcaatct gaatttgaaa gtttgaatga tacaatcgag
     7741 aatacattag ccgcttttga ttctaatcca gctgtcaaaa gctatttttc caatgatcca
     7801 gcccaacata tggaacagct tcagcaacta agaacaatcc aaaaaatgaa tgactctctt
     7861 tcaaaaatcc atccaatgat cgattacgat gtattgatct ttggagaaaa tggaagaact
     7921 tttgtcggaa acgacatgct cactgccgta tctgccgatt ctttttttca atctgcgatt
     7981 gctcagagag tgaatgaacg tgctgctgat acacaaatgc tttttgcaca ccacggattg
     8041 actcttcgcg ataagaaagc acccagcgtc ttctttgtca aaaaactgaa aaatacattg
     8101 aatcacgttt acggatatgc tgttttatcg atctcatcca agcaactagc gaatcttttt
     8161 caatctgtag taaatcctga gatatcgaag atctccttca tgaacgaaga acagacgatc
     8221 atcgcatcaa atgaagaaaa tacaattggg agccgatcaa tgattttcaa aaaactaaca
     8281 gtaggtgaaa caacgatttc aaatggacag cagttgaccc gtcttcctct gtaccgccag
     8341 gattgtacat tactcagtca agtcgatctt cattctttat ctaaccaaat gggcgtgatt
     8401 tttccgatta ttttgtataa tatcgtgaca cttgcttttg ctggctcatt ggtatttatg
     8461 tatttggatc gacacacaaa atcgatctac cgcttgattg attcgctaaa acggctagag
     8521 gaagatggct taaaagctac tgttcctgta caaggaagcc atgaagtcca attgctaggg
     8581 aatacgatca atcaattgct aagtaatgct cagcatcatt atgaacagac gatccaagac
     8641 gaaaagaaga aacgttcgct tgaaatccaa actatgcaag cacagataca gccccatttt
     8701 atttataata cgctgactag cttgaaattt ttgatttggc agaaaaaaac agatgaagca
     8761 gttcaaggaa tcgacagttt cattacttta ctaagaagta cgattgggaa aaaagaagaa
     8821 gtgattccag tcaaggaaga aatcaaaagc gtacaaagtt acatcgatct tttgagtatg
     8881 cgatatggcg aaagaatctc ggctaaaata atgattcctg atgaactgca gtcattatct
     8941 atgccgaata tgatccttca accaattatc gaaaacgcct atcttcatgc ttttcacaat
     9001 aaaacgcaag gattcatcac ggtgtacggc aaaacaaaaa atcagcttct tttattcgaa
     9061 gtgattgata acggcgatgg cttcgatact gaaaatacca aacaaaaaaa tgatcatttt
     9121 tcaggaatcg gcatcagcca tgtagatgag cggatacgcc taatgtatgg cgatgcatat
     9181 ggcttaacga tccaatcagt gattgggaca ggaacgactg taacgatttg tctaccgctt
     9241 cttcctaaaa atcaatcaga atatcaaaaa taaagaatag ccaagcgctg atttttttat
     9301 tttaagttaa ataaatcaat cagtattctt gactttcatc tacgcgtctc ttctactgtc
     9361 agcactggat aacagcccaa tatttgactt tctcctccga gcatcttgat ttcttggagc
     9421 gcattctcta ctaatgtcat tggacggtcc aacaacaaat cgatcacgaa aaaatattct
     9481 cccaagctag ttttcaatgg tcgggattca atcttactta agttgatttc ccgccagcca
     9541 aaagctgcca gcattttatg aagcatccct ggccgattag caggtagtgt caagatgaca
     9601 ctcattttta ctggagctga ctgctggctg gtcatctttc ggtcaccgac gatccaaaaa
     9661 cgtgtctggt tcaactcatt atcttggata ttctcagcta agatctccaa tccaacatgc
     9721 tgtgctgttt ctaatgaagc aatcgctgct gcgtcttctt tcggatgttc tgctacatac
     9781 attgctgcag cagttgttga ttctgttgct actaaaggaa cattaggata atgcgtttct
     9841 aaaaactgtt gtgattgtgc tagtgcttgt ggatgtgaaa ggatttttgt aatctttgtt
     9901 gctggattgc ctaaaagctg ctgcttgata ggaagaacga tttcactttt aacttcgact
     9961 tctggatgtt tggaaagaag atcgattgtg tgatgaacag ctccttccaa tgaattctct
    10021 acaggaacga cagccagatc gacttgtttg cgaaacaaag cgcgcaaaca aacagggatg
    10081 gaagcatacg cagtcaattg ttctgtagga aatagctgac aagccgcctg ataagtgaat
    10141 gaactttctg gccctagata gctgacgttc atatgctttt gatcctttct ataatggtat
    10201 cgatgatctt ttctggtgtt tgattggtcg tgttgattcg atgtgtcgca ctttcttcat
    10261 accaagaaag tcgttgtgcc aataatgctt ttatttcttc tcttgttttt ccaagagcta
    10321 aaggtcgaat agacgtttca tcttgttgga tacgatcaac tagacagtcc gcttctgcct
    10381 ctaagtaaag aacaatcggc tgtgttttta aaaaacgccg attttctgca ctttggacaa
    10441 tccctccacc agtcgcgata attttgtctt catttgacag ctttcttaaa atatctgact
    10501 ccacttttcg aaagcttttt tcaccgtaat agtgaaaata ttcggcaatc gacaattgga
    10561 tatgttcctc aatctttgta tctaaatcta catactcttt ttgtaaacat tgagctaacc
    10621 ctttaccgat tgttgttttt ccagcgccca tgaaaccaat cagcaaaatg ctagccatga
    10681 gcttctcctc ctgacacaag ttctgctaaa tcgtcaaaaa atgccggata agagattgaa
    10741 acagcttcag ctccttctag ttcgcaaggt ccatcagcta tcaaagcagc tacttgcagc
    10801 atcataccga ttcgatgatc gcctctgctg gacacttttg cgccatgcag aggtgtcggc
    10861 ccatggatga tcagaccgtc tgctgtcgct tttatctttg ctcccatttt ctgtaattct
    10921 tctgccaccg catcaattct gttggtttct tttactttta attcttctgc gtctttgatg
    10981 atcgtgattc cttctgcttg agtagccact aaagctagaa tgggcaattc atcaatcaga
    11041 cgcggaatga tttctccacc aatcgttgct ttcttcaaat gactcgtctt gacacttaaa
    11101 tcagccgatt ggttctgttc attgtgattc gtctgggtaa tcgctgcgcc catctcctcc
    11161 aacacatcta agataccagt tcttgttgga ttgaccccga cattttttaa caacagttgg
    11221 ctttccggaa caagaagtcc tgctgctaga aagaaagcag ctgatgaaat atcacctgga
    11281 acagttatct gttgaccagt taatttttgc ggtccggtca ctatgatcgt tttatcttcc
    11341 actgtgatcc ttccgccaaa ttgacgaatc atttcttccg tatggttacg ggaacgttct
    11401 ttttcaacga ttacggatgt gccttctgct tgtaatgccg caaacagcaa agcagatttg
    11461 acttgcgcac tggctacagg catattgtat cggataggtg ataaagagcg ggttcctgtc
    11521 acagtgattg gtagatactg gtcattttct tctccttgca agtcagcgcc catctcgcgt
    11581 aaaggttcca tcactcgccc cattggacgt ttagacaacg aagcatcccc gaacagagta
    11641 gtcgtaaaag gctgtccagc caaaataccc atcaataaac gtatggtcgt accggagttt
    11701 cccatgtcaa ttgattcggt tggttggaca aatgagtggc taccgcgacc atgaatcttg
    11761 atcacctctt cctcttcttc gatctcgaca cctaattgtt ggaaggcatg taaagtactc
    11821 agacaatctt ctgcgcgcaa aaaattttgt atagtcgtcg ttccttcagc aattgctcca
    11881 aacatgatgc tgcgatggga aattgattta tctgctggta tcctaacagt ccctcttaat
    11941 ccatgtattt gttgtaataa ttgcatttat ccagcctcct atttgatcca gcaatgataa
    12001 ctggtattag acataatgat tctttttgct tgtaacaaat cctgttggtt tttaaaactg
    12061 atttgcagta ttcctaaaat atcttccctg gtctcttgga tcttcaaatt aatcaaagaa
    12121 actcccgctt ttccgatcag tccagttact tctgcaataa cgccaggttc atctggcaca
    12181 tcgataaata aatcaaaaaa ggcaggaatc gcgccttgct tatggacagg caactggtct
    12241 cgactttctt ttgcccgatt gaaaaaagaa aagatttccg attggttctc agtcatgatc
    12301 cattctgcta tttgtgtcat ttcttgttgc caatctgcca atcgttccaa taatatctgc
    12361 ttattgctca acaaaatatc tgtccacatg acaggatctg aggaagcgat cctagtgata
    12421 tctcgaaagc ctcctgctgc cagttgtttg gctcgcggat actcttgatt gaattgttct
    12481 gcctgattga ctaagccagc tgccagaata tgtggaaaat gacttagcat tcctgttata
    12541 cggtcatgtt ctcttgctgt aagagttaca tattttgctt tggttccgca aaatagttct
    12601 tgcagttttt caacatcttt tttcgtcttt tccaaaggtg taaagatgta ataggcattt
    12661 tcaaatagtt tagcatctgc tgcctgtata cctgatttat gagaaccagc cattggatgt
    12721 ccaccgacaa accgaagatt tttctgctca gcggatgtca cgacttgctg cttcgtactg
    12781 ctgacatccg taatgatgac gtgatcagac aagttgattc tttcaaggac atcaagataa
    12841 gctaaggttg tacggacggg aacggctaat aagataacgt ctgcttgttc tgcacctgtt
    12901 tcaaaatcac ttgcaacgta atcaacgatc tgctgttcta ctgcttgctc acgggttttt
    12961 gactgactat cccaaccaac tactatcagt tctggattgg tctgacgaat acaaagtgcc
    13021 atagaagcac caatcagtcc aagccccacg ataaaaacat gttgtgccat cttatttgcc
    13081 ccctttttag taatgtttca agtagtcacg gtactccttc actgcagctt tcaactcaac
    13141 aagcgaatca cttgggaatt ttgacaatag ctctgtcgct accacagtgg ctacaacatt
    13201 ttccgcaacg acgctagctg caggaactgc tgtactatcc gaacgttcta cacttgcttt
    13261 gtaaggttct tttgtatcga tatcaacact ttgaagtggc ttgtacaatg taggaatggg
    13321 tttcatgacc ccgcgaatga caagtggttc gccattggtc attccgcctt cgaagccgcc
    13381 tagattattg gtacgtcgga aatagcctct ttcttcttcc cataggatct catccatcac
    13441 ttctgagcct ggtaatgaac cagaagtgaa tccgacccca aattctacgc ctttaaaggc
    13501 attgatacta accacagctt gtgcgatttt ggcatctagc ttcgtgtccc attgtacgta
    13561 gcttcctaat ccagctggaa cgcctccaac gagcacttct acaattcctc cgatcgtatc
    13621 tccatttttc tttgtctgat cgatcaaatc ttttattctt tgctcaactg ctggatcaac
    13681 aactcgtact tctgacgctt cagaaatttc tttgatttct tgaatcgaga tatttccagg
    13741 tatttctgct tggattccac caagacaaat cacgtgtcct gctacgatga tgtcaagttc
    13801 ttccagaatt ttcttggcaa ttgcgccgat tgctacacgc atggtcgtct ctcttgcaga
    13861 ggaccgttca agcacattcc gaagatctcg atgatggtat ttcataccgc ctaccaaatc
    13921 cgcgtgtcct ggacgcggac gtgcaactcg ccgcagtgat tggtcttttt caggcacttc
    13981 tgctacagac atgacagttg tccaattttt ccaatcttta ttttctacca ctaaagtgac
    14041 aggtgaacca attgttttgc cgtgacggat gccagacgtg atccgaacac gatctttttc
    14101 gatcttcatc cttcctcctc tgccgtaacc agcttgtctg cgagcgagtt cgtgattgat
    14161 ttcttcttct gacagaggta atcctgctgg tagtccttca ataatggctg ttaattctgg
    14221 tccatgggat tctcccgcag tcatataacg catcttacag tcctcctaag taatctttga
    14281 atgtctctgt gggaacagga acgatttttg cttgcccaat cttttctagc aagatgatat
    14341 tcaattggct tcctcttgct ttcttatcat gggttatcgc gtggaataat tcatctggat
    14401 gacttagatc gtacgtaatc ggcagatgga atttttgaag tatcctttct aaagcagctg
    14461 tcgatccagt tggagacaag ccgatttttt ctgcatgaga agtgatggct accattccaa
    14521 tagctactcc ttctccatga ctgactaccc catatcctgc tgtattttca attgcatggc
    14581 caattgtatg accaaaattt aaaataagtc gatttccttg gtcaaagaca tcttcttcca
    14641 ctacttttcg tttgatttcc aacgtagcag taatgattgg aacagcatgt tccagaagat
    14701 ctttttcgtc tttcagctcg ttcagctgtt tccaaagcgt tgcatctgct atagctgctg
    14761 atttgatgat ttcagcgatt ccttctcgta aacgacgtac cggcaaagtt tttagaacct
    14821 caggatcaat caatacacca gctggctggc taaatgttcc gacaaggttt ttcgctttgt
    14881 ttgtattgac tgctgtcttc ccgccaatac tagaatctac ttgtgcaagt agcgtagtcg
    14941 gaatttgaac gaaatggatc ccacgcatga acgtcgaggc cacgaatgct gctagatcac
    15001 ccaccattcc tccaccaagt gcaagtatcc catcgctgcg ggtaaaatca ttatctgcta
    15061 aaaaatcgta taactcagct gcttgttcta aagatttact ggtctcaccc gccggtacga
    15121 ttgaaaggac tggttcaaaa ttagcttgtc tcaattggaa catgattttt tctgcatata
    15181 gtggagcaac gtttgtatca gtgatcacag ctactttttg aggtctccaa atcgtttgaa
    15241 gccatgatcc agcctgttct agtgcgcctt tttcaatatg aatatcgtat ttttggttat
    15301 caacatgtac agtaagcatt cgtttcactt ctttctttta aaaagctgga attaatcgag
    15361 tgtctgaatc ttttccattg cttctttcat gatcgcaact tctttcatca tgcgtgagta
    15421 agtcttttca ttcaacgatt ggggaccgtc tgaccaagca tttgcaggat caggatggat
    15481 ctctacgatc atgccatctg ctccgcttgc cacacccgca cgagccattg gtggtacaag
    15541 ttcccaaatt cctgtaccat gacttggatc aacaatcaca gggaaatgag ttaatttttt
    15601 catcaatggg acagcactta aatcaaacgt attacgtgtc gctgtttcgt aagtccgaat
    15661 cccacgttcg ataaaaatga caggagattt gtcttcgact gcaatgtatt ctgcagcatt
    15721 aagccattca ttgatcgtac cggaaattcc acgtttcaat ccaacaggtt tacctgtttt
    15781 accgacagca gatagcagtt taaaattttg catattacgt gcgccaatct gtaggatatc
    15841 gctgtactca gccaccatat cgatatgccc ttcatccatc acttcagtaa tcactttcat
    15901 tccaaattca tcagccgctt gacggatgta tttcaatcct tcttcttcta aaccttggaa
    15961 agcatatgga gaagtacgtg gtttgaaggc accacctcgc agaatttttg cgcccccggc
    16021 tttggccatt cgcgcacatt ctctgatttg gtctaatcct tcgatagaac aaggaccagc
    16081 catcgtcacg aaactgccgt caccaatttt tactccgtct acatccacga ctgtattgcc
    16141 aggatggaat tctcgagaag tcaatttgta tgtgtttaaa atacgcacag cattttctac
    16201 tccttcatag cgtgtaaatg ctccttcttg taaacttcgc gtatcacctt ttagtccaat
    16261 cactactcgt tctgttcctt gatcgattga gactttgaat ccttcttttt cgatatgatc
    16321 gacgactttt tttatttgtt cttccgttgc ttcttttttc ataattacta tcattgtttt
    16381 tcccgctttc ttttgtttac ttatctatct tggctgattt tctctacttt ttcaaggtgg
    16441 ttttacatta attcctacta aatgaccgtg tcctgttgtt ctcattttcg ctccttcttt
    16501 cctacttatg acccagataa tcaacaaaaa aagatgtccc tgtaggaaat tgcctctaca
    16561 gtgaacatct tcaacgagcc ttaaaaagcg ctgcaaaaat ataaaatagc cactatagat
    16621 gaatttctct acgtggctgt aagtcgtgtt taaaaatctt tagccatcat agatacaaac
    16681 ctggtttgtt tgtacccacg aagtcatgaa tacaaaccta tctaaaatag ctaaagtaat
    16741 acgtattcag ttttgtaagt tttctagatt tcataaaaaa tcgctccaaa ctgatgtaag
    16801 aattgtacct agtatagaca taaaaaaatc gactgtcaat catttttatg aaactttttt
    16861 taattttctg atagttaact ttgtttttca gaaaaataac aattggtttt attgaagaaa
    16921 atagtcatga acaaaagtat ctgagaactt ttttactctc ttgttcatga catatagaat
    16981 aaagcagctt tgtcttgtaa actccgtttt tcaaataata atctaaagat tgctactgaa
    17041 taataaagcc gatttcatgg tcctcttcgc tgctgattcg gtaaggtgac tcaacatccg
    17101 ctccccagct attgataccc ccgactcctc ttacttttgc aaaaatcgta agaaccgttc
    17161 gtctttctgg cggtagttct tctatatgtg ttgcctgttc cagttcttct gctttgaatg
    17221 gtaaagcgga aaaagcaaat ggcgtatcct gctgttcaaa tcgcaacgaa aaatcttctt
    17281 gatctttatc cgtattatcc aacgtttgat ttcttgttag ttctacccat ttcgtttcca
    17341 tatgcatgcc gcattcttgg ggaacaaggt atggggaaac ggggagtcct gccactttat
    17401 aaataccttc gacaccacca gccatccgat ccggatatgt ttcccctgat aggccttcat
    17461 actcaaaaaa ttttgctttc gttggcataa tgaaccttaa acccaatgcg ggtaattccg
    17521 gtaatccttt ttgtccataa taatgcatat tgacaagcat tttaccctct ccagcgattt
    17581 ggtaggaaat tgtcacttgg gtttttggta ctgtgttgat ttccagtgta aaaatgattt
    17641 caaccgtttc agcgtattca ttacttgaat attgattatt tactggagca atcggcaaat
    17701 cgatcttttt attgtccacc agtacttcta tttctttgac ttttggatac aatccggctc
    17761 ctaaccacat accggattta aatccaaatc cgctacctct gtcattatcg gtcgttgctc
    17821 gccaaaaaac tggaagtgtt tcccggtata gccattcttt tccgttttta ttgagcgatt
    17881 ccagtccgcc tcttgtataa gaaaaaatat aatgcgtatt ccccgatttg acacctaatg
    17941 tacagtcgcc gaagacgatt gcaacttttt ccgtatccat atgactttta tttactgtat
    18001 ttttcataat aatatcttac ctcctgcatg gcgggttttt cttgtccatc tgcaaaaacg
    18061 atcccattcc ctgagaattc atagtcactt ggtttatcgt caaagtcacc gccatagcgc
    18121 atcacttctc tgcctgtgat tggatcagtc acttggatcg cctgatcttt aaaatcccat
    18181 atatagcctc cttggtacat ttcataacga tcaagcaacg tcatgtaaga atccattcca
    18241 cctaacgaat tccccatgtc atgcatatat tcacataaga tgaacggttt ttccgcttta
    18301 ttttcaaggt agtcaatgat ctcttctggt ggcgcataca tgcagctttt tacatctgaa
    18361 atcgaatctt cgaatgctgg atttggaaaa ttcccttcat aatggacaag tcgatcgggg
    18421 tctacttttt tgaagtaatc gttcatttgt cgaatgtttt cttccgcata agattcattt
    18481 ccaagagacc aaaaaagaat acttggatgg ttttttaatg tttcgaaatt cgtttttgct
    18541 cgatcaagaa cagccgcctg ccactgaggt actgcacctg gtacgttgta agaaggctca
    18601 aatgcgccca ttttttgcca tgagccatgg gattcgaggt tcgtttccgc catcacatgg
    18661 attcctgctt ggtcgcataa ataataccaa gtgatctggt tggggtaatg agatgtccgt
    18721 acgccgttga tctttgcatt ttttatgata gccaaatctt tttccatatc ttctatagtg
    18781 atggcgcgtc cttttttcgg atgccactca tgtcgattta ccccgttgat aatcatccgt
    18841 tttccattga agtagatgat tttatctttg atttcgattt tacgaaaacc gaaggaatag
    18901 tcaatatact ccgttactct tttttcttcg tctatcgtct ccaattcaag cttatataaa
    18961 ttgggctgaa aatgccccca cagtgccaca ttttcaaatc catcgatgcg aatcgtcatt
    19021 tgctctttta acggatattg ctgttgatag atttccttcc catcaggatc tagtatcgct
    19081 acattcagct gactgttttt ggctacttgc tcaaaagaca aatctacttc aagtttgcca
    19141 ttttgacatt tgtcatctaa gcgaggtttg atccaaagat cttctaaatg gttttgtcct
    19201 cgagcaacta agaagacatc tctgaagatc ccaaaaaatc ggaaaaagtc ctgatcttcc
    19261 aagaatgcag ctgtacttct tttgtaaacc tccacagcta gaatattttc ttcttccaaa
    19321 taaggtgtta aatcaaattc agacggagtg aacgaatctt cggcataccc gataaaatga
    19381 ccattcaacc aaataaacat tgcttgttct acaccagcaa aatgaatcga gatttgtttt
    19441 ccctttagac cttcttctaa tcgaaaagtc gtcaaatacg aaccaaccgg attgtcttta
    19501 gcagctgaaa agatcccttc cgcctctttc tcagggcttg tcgtgaaagg gggacggcga
    19561 aatacatgtc cctcccacgg atacatcgta ttgatgtaat ggatcttgtc ataatttgct
    19621 aattcaatat gactaggaac agtgatttca tcaaaagaac aggcatcaaa atctttttta
    19681 taaaatgttt ttggtcgtac cgaaggattt ggcgaataac aaaaacgcca tgatccattt
    19741 aaagactgtt taagggagga tgtggtttct acttcttccc ttgttgcata acaaacatga
    19801 tcgctatgtg catttgattg gttcactcta aaaacttgtg gatcatctaa ccattttata
    19861 tttgctttca tacttttttc cttctctctc ataacttttt gaaatgaagc agttctgttt
    19921 ggaaatcacc ttgaaaatcg gggaagtaaa gtccaactgt catcaattcg gcccccgaaa
    19981 atatccgctt cgaggtctgt tcctgataaa gtgcctcatc atctaacccc atcagatata
    20041 cttcatggaa cgctggttga gctgaagcta gtatcctccc taaaaacaca atagcttcat
    20101 cttgtttagg agacacaaac atccatgcgg caatattttc ttcaaaagga ctcgccaaac
    20161 gataataatg gccatactgg atcaccggac gaatcgtttg ataggatata atttggtctt
    20221 ttacttgctt ttcttcttca ggagacagct ttgtcaaatc cagttcataa ccaaaaacag
    20281 cgctcatagc tgtttctcct cttgttttca acgaagtaga tctgtcggtc tgatgatttg
    20341 gtacagctga tacatgagct gtgatggacg aaatcggata agcaaggctt gttccatatt
    20401 ggattttcat acgctccact gcatccgtgt tatcacttgt ccatgactgc ggcatataat
    20461 aaataaaccc ggcatcaaac cgtccacctc caccagaaca gccttcccat aagatttccg
    20521 gataatcagt tgtcaaatgt tcaagtagtt cataaagccc gagcatataa cgatgcgcta
    20581 cttctccttg tctctgtggg tcaagggtaa tcgaatagac atctgaaagc gaacggttca
    20641 tatcccattt gatgtagtca agaggaatcg tatctaaaat ctttctcatt tgtttctcaa
    20701 tcgtctgccg aacatctaac cttgtaaaat ccagtacatg ttgtgatctg gaagcagaag
    20761 gcattctacc tggctcctgc ataagaaaat caggatgtgt tcgatacagt tcagaatcaa
    20821 ccgagatcat ttcaggttca aaccataaac caaatttcaa tcctttgcta tgagcataat
    20881 ctgctatttc acgtaatcca tttgtcaact tgccttcata ctcgaaccag tctcctaatg
    20941 aagaattgtc cgcatctctt tttccaaacc agccatcatc taatacgaac atttcgatgc
    21001 ctagctcctt tgcttcatca atgatcgcct tgattttttc actattgaag tcaaagtaag
    21061 tggcttccca gttattgacc agaatcggcc gttcagcaaa ttgatgacga cctcttgcga
    21121 ttctttcacg tagcaactca tggtggatct gactcatttt gttcaatccc tgacttgagt
    21181 aagaaaggat catttcaggt gtttgaaaag actggcctgc attcaattcc cacgaaaaac
    21241 gatggctgtt gatcccaccg acgactctca attgattgat ctgatctttt tcaagttcga
    21301 aagaatggtt gccggaataa acaaattgaa gtccgatcgc ttctccagta ttttctgtcg
    21361 tatgatgatg aactaaagca ataaagttat tcatatggtg actgctcgtt cctcgacggc
    21421 tttcaaatac ttttgttcca gataaaacag attgccgtga aatctgtctt tcattgacat
    21481 gtgctcctgg caaagcaatg acttcatcaa atcgcctagt atgggcaaag tctagctgga
    21541 aactagccgc tttttctagt ttgattgttt ctccggtttc atttcttatc tttacagatc
    21601 gtgtgatcac tgctctatca gtaaatatgg tatagaacaa atcgaaataa atcgctgttt
    21661 ctctatcctt aagtgaaatc accaacgttt gtgcctctga cttgtccatc acataagttt
    21721 gaggcaagcc ttcgatctct ggcttcccat ctgtaatata atggctgtca tatctcagat
    21781 ccagtaagtc tgatccattc ttgcgctgaa tgatcgatgc gggcgttcga aagtccatgc
    21841 tgccatgaga agagtattct tgcggtagtg tatctttcga cagagaccgg tcaggattta
    21901 agggaacatt tcctgaaaat ccacgatctc ttcggggata atttttattg tctttgtact
    21961 gcttgactgg tttgccaaaa taaatatgcg ccaatacatt gctttcttct atctttatca
    22021 gataagaaag aaatgtattt cgtaaatgga agactcggct cttttcatca aataagatga
    22081 aatttttatc tgtttttcta tgcgtaatat ccatcatttc actcccaaca aaacggaaac
    22141 cgtttacttt tttttataat tcctgtatac tgtttactaa acaattttgt caataaaaat
    22201 tttttatttt tttgaggagt gtgtaaaaat ggttggaatt agagatgtcg cacgaaaggc
    22261 aaatgtctct cctgctacag tttcccgtgt gttgaataac gatcagacga tccatgtggc
    22321 tcagactact cggcaacgga tagtagaggc agctgctgcg ttggattatg acatcagtac
    22381 tcgtaaatat atcaaaaaga gaatgccttc gattggtgtg atctctacga tcggcaaagc
    22441 gagcgaagaa caggatatct attacaaaca tttgcgagta gggatggaag aagaagctcg
    22501 acgtctgcat ttagggatga atcgaatcta taatctatct gataatccta aacaatggga
    22561 agatctagat cagttaggtg cggtgatcgt tgttgggact gttacgaaag aatcgattac
    22621 cgccttgtta aaacaaaaca tgcaaatagt cgtcgtggat aaccctgaca tccagcaaga
    22681 ggtcgatatg gtgtatgcag atcttgaacg gatgactaaa accgtactgg aattgttctt
    22741 agaaaatggt cataaaagaa tagcctatat cgggggatat aaagtcgata tggatgaatt
    22801 cggtgggaag cagatttcta taaatgaaaa gaggatgcgt gcctatcagc attttatgat
    22861 cgatcatcac ttagaaaaat atatgtctta tcatctaggt ggctggacag aaaaacaagg
    22921 tgaggtactt gcgactaatc tattagaaaa tgaatcccct cttcctactg ccattcttgt
    22981 cgggagcgat ccaatggcaa ttggtgtcta tcgggcattg aaacaaaaaa atatccaaat
    23041 tggagaagag attgttgtag caagctttga taatatcgag acaatcgcaa acctctcccc
    23101 tggcttgacc agtgttcaaa tcaatgcgaa atcaattggg caagcagctg ttcgaatggc
    23161 attggagcga attgacggta tacgggacga accaatcatt ttgacttatc ctgcacgatt
    23221 gattgttagg gaaagtttta tcccaagaac aaaaaggtat tgaaagagag gttgtgaaaa
    23281 gccgcttcgt ttcttcatac tcaaaacgaa acggcttttt cacaacctct tacttatact
    23341 tattcacaaa atccaatgtc tgctggtaac aattttgcga ttcttttagc gtgtaatcaa
    23401 attggtattc atgggtaatc tctttttttg tatctgtata aaacaaggat tgcacgggta
    23461 cctttaattc tgtcaaccga ttttctaatg cttgaccttg ctcctggaat gagtatgcat
    23521 ttccgtcagt tatatacgtt ggcggaaatt ccttagaaac atggtcagcc acactagctt
    23581 ctttcaattg ctcactcgtt ttccaatctt ttgttccaat caacgaccac gcaacggttt
    23641 tgacgaaaaa cttcataaat cgatcatccg aatgctgctg tgctgttagc ttcaaatcaa
    23701 caggtccgca ataagaaatg gttcctttca gactatcttt tggaagtagc tgtttgattt
    23761 tcatttgttt agcatacgca tcattcgttt gtatagctgc atattgcaat gcgatttgtg
    23821 aacctgcact atctccacca aaaaataatt gttccatgtc tagcaatttg tcgtcttttt
    23881 cttcagctaa taaatctttg atcagttcat tcacttgaag aacttgatta gggtattctg
    23941 aatcaggcgc taattcataa ttcatcgcaa ccactgcgat gttggcgtct gaaactaatt
    24001 ttgtcgcaaa ttctttgaca cccgatttat ctccgccgac gaatcctcca ccatgtaccc
    24061 aaaatagaac aggaacaggc cccttgctgt tttttggata gtagatgtca taagtattct
    24121 ctttatactt ggactcatac gtcaaatctg tttttttcgc cacgtttttt tctgcttgat
    24181 cgaacgtttt tttatcagtg atttgaacag tatttgaaaa catatggttg atcaagaatg
    24241 ctccaggtct gggggaaagc tggaaggcaa gaaagattgc aaacgcgatc ccaagaatga
    24301 ttccgaagaa ccatccgata aatttaagaa ttttcttttt cataaggctc cctcagtagt
    24361 tagtattaag cattctgatt ataccatagt gttatttcct atcaagctct cctagatgat
    24421 ttaatgattt tccccaaact tcttctgcaa tgcttttgac aaaagaaatt ttttccattg
    24481 ttcttcaaac aaaacatttt cttcttctgt tgaagcaaat ccacattgag gacttaagca
    24541 caaacgatcc aaaggcagat atcgtgtcgc ttcgtagatc cggtcaatca cttgctggtg
    24601 ctcttccatt tcgccatttt tcgttacagt caaaatctaa gacaactaat ttatcttcag
    24661 agactttctc taaaagaaca aaactcccag cacgttcact atcgtattct aaataatagg
    24721 cacggacatt ttcttgagta aaataatggg tctgctacgt cgtcatcccc cctgatgccg
    24781 ccaaaatcga ataataattt ccgcgtcaaa catgtatatt tagtgttgaa tcatttgaga
    24841 atatgaaaat aaaaatcttt ttgatcaatc agttaatcaa attgaaataa cgaaaaatct
    24901 gagacagaat gacgttcatt ctgacccaga tttgattttg cacaactcaa cagtccttat
    24961 tggcaaatcg ccaaaataat gtagactaac taaaaagact aagaaacaat aacaaaagtt
    25021 aacacttctt agtctttctt tagcacgtct atttattaaa gattttaggg ccttttctca
    25081 tgtttttacg atcattctta ggtgtttcca tttgtttgga tcggcctcca ccttgtttct
    25141 ttttgatcaa ttctttcatt ttttcttttt ggtccatttt tattctcctt taacgaatga
    25201 agcgactaat tcgatatgat gggtttgcgg gaaaagatct acaggctgaa ctttcttcac
    25261 ggcataacct gcttctgcaa ataacttagc atctcttgca aaagttgccg gattacatga
    25321 gatatagacg attcgttctg gtgacatttc gattgctgat tcaataaaat gatgatctaa
    25381 tccttttcgt ggagggtcaa ctacgatcac agatggtttg atcccttttt cttgccattt
    25441 tttcataact ttttctgctt ttccgacttc ataatgaaca ttttcgattt tattatttaa
    25501 agcattgaac tgtgcatctt caattgcttc tggaataact tccatcccat acaccttatc
    25561 cactctgtct gctaaagaca agccaatcgt acctattcct gaataagcgt cgatcacaat
    25621 gtctttttta tctaactcgg caaactcgat ggctgtttgg tacagatact ctgcttgttg
    25681 agtatttact tgataaaatg actttgctga gattcgatag gtttttccta atagctgatc
    25741 ttcgatatag tctctgccat ataaaacttt ttctttgtct cctaaaatca catttgtttt
    25801 ttcttcattg atattttgga tgaccgaaac cacttctggc aattcttcat gaatagcttc
    25861 agcaatttcc ttggatttaa agaatttttc tttacgagta actaaaacga ccatcatttc
    25921 atgggaataa tgaccacgac ggataatgat gtgacgaagc tgtccttcat ttttttcttc
    25981 attatatcct cgtacttgga accgttgcaa gatatctctg actactttga ttgcttgatc
    26041 gatgacagga tcttggatat aaaaatcttc gatagggact agatcatggc tattcttttt
    26101 gtaaaaaccg gtggctaact gtccatcgat acggcgaaca gggatctgtg ctttattacg
    26161 gtagccgaat ggttctgcca ttccgaccgt tggaagtaca ggaacttctg gcatcttagc
    26221 aatcttcgcc attacatgct ctacttgttt ttgtttgaag acaagctgtt gttcatactt
    26281 caaatgggct aaaggtgcga tacctatgcg aagtaatgca ttgttttcta caggctgacg
    26341 atcaggtgat tgtgtaataa atgtttcgac ttttccatat ccaaacttat tgcctgcttt
    26401 tactactagt atttctactt cttctccagg gagtgcattt tcgataaata atggataacc
    26461 atcgattttt gctaccccca ttccttcata actcaagtcg acgatttcga ctgtatatcg
    26521 ttcgtttttc tttactattt gctgttctgt catattttgc tcctttaagc ctatttttca
    26581 atcctactga actctactat aaatcagcta attacacaac taaaaccggc actgacctta
    26641 ctacgttcag caccggcttt tgattaattc ttttgattag gtgtttcgtc tgctattttt
    26701 ccgtcaccgt cgatatcttc atccgttaaa cgttcgactt cttttacgaa ttctttgctg
    26761 acaacctcta attcttcttc atctgtacct gtgattgctt catcaggaat ctgatcggta
    26821 ttggcaaaga actcaatgtg ttgatgaaga ttttggaaat gcatcggtgc ttccccgccg
    26881 tattcaccat ctaagttgat cattactggt cgattgatat catcttctgt ttcaacgaac
    26941 aaactgcttg ttttggtata gattattctt ggatcatcga catgtcgacc gccattcagc
    27001 atgagtgctg caatatgcaa gatctcaaag atatttgctg tcttcacaat gattaaagaa
    27061 aacttcccat catccaattt ggcatctggc gcgatctgtt cgaatcctcc aacagagttc
    27121 gtcagcccta ggaaaaacat cgaagcattt ccgatatatt ccccttcatc atacttcaag
    27181 cgcattttga ttggctttac tcgagggagc atctctgctc ctttcgcaag ataagcaaga
    27241 taaccaaaaa tactctttag ttctgatgga acttcataag tcaattctgt caaatagcca
    27301 cctgcagcga tattgatgaa gtaactgtta tcagaaaggc cgatgtccat ttttacggtt
    27361 tggtgttttt ttatcacttc tgctgctgct ttaatattat ctctaggaat ttttaatgct
    27421 cgagcatagt cgttcgttgt accagctggg atgatggcca ttttaggacg gtgttcaagc
    27481 ggagcgattc cattaaccac ttcgttgatt gtgccatcgc caccagctgc cactactaga
    27541 tcaaaaccta gttctgcgac tctacatgct tcattcttgg ctgaatctgg ttctggtgta
    27601 gttgcatagg cgctggcttc aaatcctgct tcttctaatg cctgtaggat atccgccaaa
    27661 ttttttttca ataattcttt tcccgaggtg gggttataaa ttacgcgtgc ttttttcatt
    27721 ttcagcctct atcgtttcgc taattcatct ttaagtagct tattcacgac accaggattt
    27781 gcttgtcctt tagttgcttt catgatttga ccgactagga agccaaccgc acgatctttt
    27841 ccatttttga aatcatcgac agattgttgg ttgttatcca aaatttcatt aatgattggc
    27901 agtagtttgt tcggatcaga taattgcacc caaccatttt tttcaactac ttcttgggct
    27961 tctccgccat ttgtgatcaa ttcacggaag acttttttgg cgatttttga gctgatcgtt
    28021 ccatcggcga tcaaacgaat cattcctgct aagttctcag gtgtcaattt tgtctcagct
    28081 aattccagtt tctcactgtt caagtaagca gaaacttcac ccatcaacca gttagatgct
    28141 tgcttggcat ctgcaccatt ttccaaaaca gcttcaaaaa aatcagacat ttcttttgta
    28201 agtgtcaata ccattgcgtc atactctgga agtccaaact cacggatata gcgttcgcga
    28261 cgagaagctg gcatctcagg caaactagct tttacttttt gaatccattc gtcatcgatc
    28321 acgaatttcg ggatatcagg ctctgggaag taacgataat cactagaccc ttctttgaca
    28381 cgcatcaaaa ctgttttatt cgttgtttcg tcaaaacggc gtgtttcttg ttggatttct
    28441 cctcctgaaa gaagaacttt tgcttgacgt ttttcttcaa aagctaaacc ttttttcacg
    28501 aaagataaag agttcaagtt tttcaattct gctttcgtac cgaactcttc ttgtccataa
    28561 gggcgaagag aaatattggc atcacagcgc atagagcctt cttccatttt cacatcgctg
    28621 acttctgtaa attggataat tgatcgcaat gcttctagat aagcgtatgc ttcttctggt
    28681 gaacgcatat cagcttcaga aacgatttcg atcaaaggcg tgccttgtcg gttcaaatca
    28741 acgtaagaat aaccgccaat accatgcatg ttttttccag catcttcttc taaatggaca
    28801 cgttcgatcc ggattttttt ctttttacct tctacttcga tttcgatcca tccgtcatga
    28861 ccgattggtt gatcaaattg ggaaatttga tatgcttttg gattatctgg gtaaaagtaa
    28921 tttttccggt caaaatgagt atctttagag atttcacagt ttaatgccaa agctgctttc
    28981 atcccgaatt ccagcgcagc tttattcatt actggcagca cgcctggata accccagtcg
    29041 atcacgtttg tattgctgtt tggttctgca ccaaaatgag ctggcgctgg agagaaaatt
    29101 tttgagttgg tttttaactc gacgtggact tctagtccaa tgactgtttc aaaattcatt
    29161 cgtctttccc tcctaaaata gctggttgtt gtttatggaa ttctgttgct tgttcaaacg
    29221 catatgccgc ttgatacatc gttttttcat caaaataatt accgatgatc tgcaatccaa
    29281 ctggtaatcc ttctgataaa cctgccggta cagacatacc tggaagtcca gccaagttga
    29341 cagggatcgt taagatgtca ctcatgtaca ttgtgattgg atcgttaatg ttctcaccga
    29401 ttccgaatgc aactgttggt gaagatggcc cgatgatcaa gtcataatct gcaaagactt
    29461 tttcaaaatc tcgtttgatc aatgtacgga cttgtcctgc ttttttgaag taagcgtcat
    29521 aataacccgc gcttaaggag aatgtaccta acatgatacg gcgttttact tcttcaccga
    29581 atccttctga acgtgagttg acatatacat catctaatga tttcacattt tcagagcgat
    29641 aaccgtagcg gataccatcg aatcgttgca aattggaact tgcttctgat gaggcaatga
    29701 tataataaac agccacaccg tattttgaat gaggcaagct gacttcttcc acaacagcac
    29761 ctaatgaacg gaatgtttct gctgctttca atacagcttc ttttacgcca ggggccacac
    29821 cttcgcctaa gtattcttta ggtaaagcaa tcttcattcc tttgatgtcg cctgtcagtc
    29881 cctctgcaaa atcaggaact gaaacgcctg aagaagtacc gtctttttcg tcataaccgc
    29941 tgatagctgt caatgctaat gcattatctt tcaccgtacg ggtcattggt ccgatttggt
    30001 caagagatga agcaaaggca atcaaaccaa aacgtgagac acggccataa gtaggtttca
    30061 tccctacgat gccgttaaaa gctgctggtt ggcggatact tcccccagta tcactaccta
    30121 aagaaactgg aatttgtcca gctgcaactg ctgctgcaga accaccagaa gagccaccag
    30181 gtacttttgt tgggtcccaa gcattttttg ttttcttgaa ataagaagtt tctgtacttc
    30241 cacccatagc aaattcatcc atgtttagct taccaacagg gatcatatct gcttgataga
    30301 ttttatccat gaccgttgca tcataaattg gttcaaaatt gtaaaggatc ttagatgcag
    30361 cggttgtcaa gatatctttt gttacgatat tatccttgat accaattgga ataccagcaa
    30421 ggacgttgct ttcatcaatc cctttttcat ctaaagcttt tgcctgttcc aatgcttttt
    30481 cttcgttcaa tgtaatgaat gcctcgacct caggctctgt ttctttgatt cgttcaaatg
    30541 ttgtttttgt caaatcttgt acagtgattt ctttcgatac aagcatatga tgcagttctt
    30601 ctagagaacg attgtgtaat tctgtcatta tgcgccagcc tccccattat cgatgattgc
    30661 gggtactttg ataaagccgt cttctgtttc cggcacattt ttcaataatt catcgcggtt
    30721 ccatccagct tgcgcccgat cttgtcgcat cacgtttact gtttcaacca cattcgacgt
    30781 aaatggaaca ccttccgtat ctacttcttc cagcatttca accatatcga tgattttacc
    30841 taattggtcg gtaaaacttg caagttcttc atctgcgaac gcaagttttg ctaatttggc
    30901 aacgtgtttc acttgttctt cactaatcgc catactaccc cttctttcta aacgagttaa
    30961 aaactttgct ctctatttta acacaagagt gccgtgtctg aaattgttta ttgaaatttg
    31021 tctgaaaaaa ctcatttttc attcttctac tgagcgactt ttagccaatg catctgccat
    31081 ttgatcttct gtccaaactt cgattcctaa ttcttgtgct ttggtcagct tgctgccagc
    31141 atcttctcct gctaccacga tatccgtttt tttcgatacg ctaccagtca ctttcccgcc
    31201 aagattttcg atcgtctctt ttgcttcttc acgagtaaat cgtgttagtt taccagtaag
    31261 gactactgtt ttttctttaa aaggagactc gacttcttgc aattgggcat ttcgaagccc
    31321 tttgtattcg aaattgacgc ctgcttgttt taattcattc atcaattcat ggacttcttc
    31381 gttactgaaa taagtgacta cactgtcggc aatgatatca ccgatcgtat caagcgcaat
    31441 aatcgactca tagtcacttt tactcaatgt ttctagatca ccgaaatgtt ctgctaaaat
    31501 ttttgctgct ttcgctccga catgtcggat tcctaacccg aaaatcaacc gctcgaccga
    31561 attatctttg ctgttgtcga ttgctgtcaa aatattattt gctgattttt cttttatttt
    31621 gtccaacgtc aataactctt cttctgtcaa tttgtaaaga tcagcaacat ctgctactag
    31681 ttttttatca tacatctgtt ctagtacgcg cggccccaac ccatcgatat tcatcgcatt
    31741 tcttgaaaca aagtggttca aaccttcttt catttgagca gggcatttag gattgatgca
    31801 ccgtaaggct acttcttcat ccaaatggac aagttcactt ccacaaactg gacaatgtgt
    31861 aggcaattgg tattcttcgc tgtctttagg tcgtttatct aacacgactt gcgaaacttc
    31921 tgggatgatg tctcccgctt tataaatcag aactgtatct ttcaagcgga tatccttcaa
    31981 cttaatataa tcaccgttat gcaggctggc acggcttact gtcgttcctg ctactcggac
    32041 tggtgtcatg atagctgttg gcgttactac ccctgttcga ccgactgtcc attcgatgtt
    32101 ttcgattagc gtttcaactt cttctggcgg aaacttgtaa gctgctgccc atctcggtgc
    32161 tttgatagta aaaccaagtt gatcttgtag cgagaattca ttcactttga tgacgattcc
    32221 atcgatttca tagggcaaat ccacacgttt gtcatgatat tcttcaatgt atgaccacac
    32281 ttcatcaatc gaatgacata gacgtttttc ccgattcgtg tggaatccaa ttttttctag
    32341 ctcttctagc gcatcatact gggtttttgc ttgcatagga ccgaaatcag ctactgtgta
    32401 taaaaaagtg tccagatttc ttttcgctgt gattttagaa tctaactgac gcaaagaacc
    32461 agctgcggcg ttccgaggat tcgcaaagat atcttttccc tctgcttctc tgtcttggtt
    32521 caattggaca aatgaccgtt ttggcatgaa acattcgcct cttacttcga tgttcattgg
    32581 ttcttttaat ttgattggga ccgaccggac agtttttagg ttttcagtga tgttttctcc
    32641 tacagttcca tccccgcgag tcgcacctct cacgaaattt ccatcttcat aacgcaaaga
    32701 aacggaaagt ccatctattt ttagttcaca gcaatagtca actacacgtc ccactgcttt
    32761 ttgtacacgt tgatcaaaag ctatcagctc ttctttgctg aaaacgtcgt tcaaactgta
    32821 aagcggaata tcatgagtga ctttttcaaa accttctaat acttttccgc ctactcgttg
    32881 agtgggagaa tcagacgtga tcaaatcagg atattgttcc tcgattgcta ctaattctgc
    32941 gtattcttta tcataaacat agtcttccac tgttggttta tcttctacgt agtattcgcg
    33001 agaccattgg ttcagtcttg tacgaagttc ttctgctcgt ttagcagctt cgtcaatcgt
    33061 catttcatgt tccatcattt accctccact actaagcttt ttcaattggc gcaaaggcag
    33121 ccaaaagacg ttttactcct tgacttggga aagccacatc tagttctaga tccttcgacg
    33181 tcccgcttac tcggacgact gttcctagac cccatttttt gtgtttgact ttatctccag
    33241 gtttccagct gttattttcc cctccagtag cacttttaga gggaacaacc gctgtctctg
    33301 gctgtttata gctatatgct gtttttttag cgaatacagg cgttttttta ggcgtaggac
    33361 gcatgccttc gatttccagt aactcctgat cgatttcttc tacaaaacga gaaggtcggt
    33421 tatattgcgt ccgtccatat aatgttctgg agaaagcatt tgttaaatac aacgcttctt
    33481 ctgctcgcgt gatccccaca tatgcgagac ggcgttcttc ctccagttcg ctttcttcca
    33541 tcaatgctcg agataaagga aaaaccccct cttctagacc aatcaggaat acaactggaa
    33601 attctaatcc tttggctgca tgcaaagtca tcaatgtcac ttgggaagca tcttcttcta
    33661 aattgtcaat gtcggaaaca agtgccagat catttaagaa gaccgttagt ttttcttctg
    33721 gtgcatcagc gtcttcttca ttttgctgtt cgaattgttt gtcgaattct tgtgtgaccg
    33781 ttaaaaattc ctccaagttt tcgatgcgtg cttgtgcttc taatgtattt tggattttta
    33841 agtcttctag atatccgcta cgatccaaaa cctctttcgt caattctgtc actgtcaagt
    33901 atgggatcat ctgagtgact tcttggatca tctctccaaa tgcacctaat tgctgtcctg
    33961 cttttccacc gatatttgcc agatccacat tttgcgctgc ttcaagcagt ggccattcat
    34021 gaagagaagc aaaactgcgt agtttttcga tagatcctgg tccgatgcct cgtttgggcg
    34081 aattaacgat acgctcaaaa ctgattgaat cttgaggatt taccagcaca ttcaaatacg
    34141 ctaaaatatc tttgatttct ttacgatcgt agaacttgtg ccctccaacc attttataag
    34201 gaatatttgc tttcaacaga gtttcttcca ttacacgaga ttgtgcgttc gtccggtaca
    34261 agatggcaaa gtcgccgtaa ttccgatgat tgcttcgaat ctcttcttgc atccggtcta
    34321 cgataaaacg ggtttcgtct cgttcattat ctgcacggta gtaagtgatc ttgtttcctt
    34381 ctttgttttc tgtccacaag ttttttggtt ttcgattgct attgttttca atgacttggt
    34441 ttgctgcgct aaggatgttt ttcgtggagc gatagttttg ttctagcaaa attactgcag
    34501 catctggata atctttttca aaatccaaga tgttctgcat atctgcaccg cgccagccgt
    34561 aaatactttg atctgcatct cccacgacgc acaggttcct aaatctacct gccaataaat
    34621 tgactagcgt atactgtgcg tgatttgtat cttggtactc atctacatga atataatgga
    34681 atttgttttg ataatacgtg agtgaatcag gatgttcttc aaataatcga atcgtattca
    34741 tgatcaggtc gtcaaagtcc atacattgat tatattgaag ctctttttgg taagcagcat
    34801 aacattttgc cgcaatctct tcgtaataac tgccttgcat ttttgcaaaa tcctgagggg
    34861 tctgcaggct gttttttgct tgagagattg ttcctaatat acttcgagga tcatattttt
    34921 tcggatcaat gttcagttct tttagtatcc gtttcatcaa ggtcagctgt tcagaagaat
    34981 cgatgattgt aaagttccgg ttatacccga taaaatcaac atctcttctt aaaatacgca
    35041 cacacatcga gtgaaaagtc gatacccaga catcttcgcc tccagatgcg agaatcgcat
    35101 tgacacgttc tttcatttct tttgcagcct tattggtaaa agtaatcgct aggatgttcc
    35161 aaggattgac ttctttttcc tcaatcaaat aggcaatgcg gtgtgtcagc actctcgtct
    35221 ttccacttcc tgcacctgcc attagcagta atggtccgtc agtgtgaaga acagcttctt
    35281 tctgacgggg gttcatcccg tttaataatt cagctttgga tgacattaac aacatccttt
    35341 cttttatcaa tcttgttaat tatagcattt tcttggcttt cattaaacta gctagttcaa
    35401 aatcatttgt ttctttttat ggatttttcc ttgtcaattc gttgatttcg cgctaaatta
    35461 aaagaaagcg aggcgtttga atggaaaaaa aaggaaaaat caagaaatta gataagactc
    35521 agatatattt tattctaaaa ggaatatttg ttggactgct gacaggaatc gtcgttagcc
    35581 tgtttcgatt atctatcgaa aagctaagcg atttagttcg aagtatctat gaaatgagtc
    35641 gggaacagcc gatttactta attggcctag ctatttgctg catccttgca gcatttttcg
    35701 tgggttatct ggtcaaaaat gaaccggata tcaagggaag cgggattcca caagtagtgg
    35761 gacagcttcg cggggaatta tcaatgaact ggttctctgt tttatggaaa aagttcattg
    35821 gcggtgtact ttcagttggt gcgggccttt ttctaggtag agaaggacct tctatacagc
    35881 ttggcgcatc cattggacaa ggagctggac aattgtttcg gagtccatct tcagaagaaa
    35941 agattttaat ttctagcggt gcaagtagcg gacttgcagc agcattcaat gctcctatcg
    36001 caggattact ctttgtttta gaagaaatcc atcactcctt ttctccactt gtttggctga
    36061 catcatttgc ttcagcaatc actgctaact tcgtctctct ttactttttc ggcttgagac
    36121 ctgttttata tctaggtgac cttccttctt tgccgttgaa atattatggt tccttagtga
    36181 tcttaggagt acttctaggc atcttaggtt ttatttatca gaatgtcttg ctggctttgc
    36241 caaaatttta caaccgattg cctttaccag cttattttca cgggttcgtt cctttcttgc
    36301 tgattttgcc aattggttat ttatggccta gtactctagg cggaggcaat caaatcgttt
    36361 tagcttttgg acatgcttcc ttgccaatca ttatgattat cggtttgttt gttcttcgat
    36421 tcgttttttc catgatttct tacggtgggg gtcttccagg ggggattttc cttccgatat
    36481 tgacattggg ggctttaatc ggtagctttt atggaaacat tatggttgat ttcgggatgg
    36541 atccaatcta catcaaggat tttgtcgtta ttgccatggc tggttatttc acagcgattg
    36601 gaaaagcacc gctgacagca attattttag tgacagaaat ggttggctct cttaatcatc
    36661 tgatgccttt aggattggct gctcttgttg cttatatcgt caatgacctt ctcggcggta
    36721 atccaattta cgaatcatta ttggaacgtc tacttggaaa tactcagacc gcctcactaa
    36781 aaggtgcaag gacaactttt gattttccag taacggcaga gagttcttta gacggattaa
    36841 tggtacgaga cttcaattgg cctaaaaata tgctgctaat ctctatacgc agaggaaatc
    36901 aggaaatctt gactcacggt gatacgatca tgaatgtagg agatattttg gttatcttga
    36961 ctgatgaagc aaacttgcca agaatcaagc aggaaatcga ggctaaatct tctatcacaa
    37021 atttgaagca atcattttaa cgaacatgga aaaactattt tacaacaact attcatcta
//
LOCUS       DAZKLA010000014        36874 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000014 DAZKLA010000000
VERSION     DAZKLA010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.014"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(87..815)
                     /locus_tag="ACG85N_000582"
     CDS             complement(87..815)
                     /locus_tag="ACG85N_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFQ0223378.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            974..1855
                     /locus_tag="ACG85N_000583"
     CDS             974..1855
                     /locus_tag="ACG85N_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0223379.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(2092..3135)
                     /locus_tag="ACG85N_000584"
     CDS             complement(2092..3135)
                     /locus_tag="ACG85N_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0223380.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(3138..3806)
                     /locus_tag="ACG85N_000585"
     CDS             complement(3138..3806)
                     /locus_tag="ACG85N_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0223381.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            3948..6284
                     /locus_tag="ACG85N_000586"
     CDS             3948..6284
                     /locus_tag="ACG85N_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFQ0223382.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(6399..6710)
                     /locus_tag="ACG85N_000587"
     CDS             complement(6399..6710)
                     /locus_tag="ACG85N_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223383.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            6891..8366
                     /locus_tag="ACG85N_000588"
     CDS             6891..8366
                     /locus_tag="ACG85N_000588"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFQ0223384.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            8413..8997
                     /locus_tag="ACG85N_000589"
     CDS             8413..8997
                     /locus_tag="ACG85N_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFQ0223385.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            8999..9418
                     /locus_tag="ACG85N_000590"
     CDS             8999..9418
                     /locus_tag="ACG85N_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFQ0223386.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(9699..9983)
                     /locus_tag="ACG85N_000591"
     CDS             complement(9699..9983)
                     /locus_tag="ACG85N_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFQ0223387.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(10335..11045)
                     /locus_tag="ACG85N_000592"
     CDS             complement(10335..11045)
                     /locus_tag="ACG85N_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFQ0223388.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(11042..12514)
                     /locus_tag="ACG85N_000593"
     CDS             complement(11042..12514)
                     /locus_tag="ACG85N_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0223389.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            12652..13125
                     /locus_tag="ACG85N_000594"
     CDS             12652..13125
                     /locus_tag="ACG85N_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0223390.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(13278..13829)
                     /locus_tag="ACG85N_000595"
     CDS             complement(13278..13829)
                     /locus_tag="ACG85N_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0223391.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            13934..14464
                     /locus_tag="ACG85N_000596"
     CDS             13934..14464
                     /locus_tag="ACG85N_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFQ0223392.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(14947..15390)
                     /locus_tag="ACG85N_000597"
     CDS             complement(14947..15390)
                     /locus_tag="ACG85N_000597"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0223393.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(15406..16566)
                     /locus_tag="ACG85N_000598"
     CDS             complement(15406..16566)
                     /locus_tag="ACG85N_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0223394.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            17073..18773
                     /locus_tag="ACG85N_000599"
     CDS             17073..18773
                     /locus_tag="ACG85N_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223395.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(18875..19708)
                     /locus_tag="ACG85N_000600"
     CDS             complement(18875..19708)
                     /locus_tag="ACG85N_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFQ0223396.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(20012..22177)
                     /locus_tag="ACG85N_000601"
     CDS             complement(20012..22177)
                     /locus_tag="ACG85N_000601"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFQ0223397.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            22437..24731
                     /locus_tag="ACG85N_000602"
     CDS             22437..24731
                     /locus_tag="ACG85N_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFQ0223398.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            24724..25389
                     /gene="pgmB"
                     /locus_tag="ACG85N_000603"
     CDS             24724..25389
                     /gene="pgmB"
                     /locus_tag="ACG85N_000603"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0223399.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            25382..26389
                     /locus_tag="ACG85N_000604"
     CDS             25382..26389
                     /locus_tag="ACG85N_000604"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0223400.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            26411..27430
                     /locus_tag="ACG85N_000605"
     CDS             26411..27430
                     /locus_tag="ACG85N_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0223401.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(27711..29078)
                     /gene="nhaC"
                     /locus_tag="ACG85N_000606"
     CDS             complement(27711..29078)
                     /gene="nhaC"
                     /locus_tag="ACG85N_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0223402.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(29187..30608)
                     /gene="tyrP"
                     /locus_tag="ACG85N_000607"
     CDS             complement(29187..30608)
                     /gene="tyrP"
                     /locus_tag="ACG85N_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFQ0223403.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(30851..32728)
                     /gene="tdc"
                     /locus_tag="ACG85N_000608"
     CDS             complement(30851..32728)
                     /gene="tdc"
                     /locus_tag="ACG85N_000608"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0223404.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(33019..34275)
                     /gene="tyrS"
                     /locus_tag="ACG85N_000609"
     CDS             complement(33019..34275)
                     /gene="tyrS"
                     /locus_tag="ACG85N_000609"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFQ0223405.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(34387..34592)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            34855..36156
                     /locus_tag="ACG85N_000610"
     CDS             34855..36156
                     /locus_tag="ACG85N_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0223406.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(36225..36824)
                     /locus_tag="ACG85N_000611"
     CDS             complement(36225..36824)
                     /locus_tag="ACG85N_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0223407.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        11367 a         7562 c         6622 g        11323 t
ORIGIN      
        1 caaaatcagt ttgtcaaata caaaaaaact gactaaaaga tttgatcctt tttagtcagc
       61 aaaaaatggc ttctctttga tttttattat aacagtggca taatccaatt tgtccacatc
      121 cagctcagtg gcatgataac gatcattgca ggtatcatgt cagctactgg gaacattttc
      181 acgttgatca ttctaaaacc ggtagcgacc atcaagaatc cgccgcaagc tttaaaatct
      241 aagatcatgt ctggtgtagt caatggataa ataaattttg ccaataagaa caaaatataa
      301 aaaataatga attggggaat tgctatcaca gaaacgacat accctaaatt acacgcaaaa
      361 atagctgctg tgaaaaaatc aaggacagat ttagaaatca aaatcgtact gtctcctgtc
      421 atcccagaat ctaaacttcc atagatcccc gtaccacttg cacaaaatag aacaatgacc
      481 gtcactaatg tgctgataaa ttcatcgtgg gtcataccaa gcttttcact agggaagatt
      541 ttagaaatag gtacttgcat cagcgcagcc cccttattta tcccattacc taaatgaaca
      601 atcaaaccaa tcgctgttcc tatcactaaa gcaaaaataa cagccggcat atttttcatc
      661 ggcgcaatgg aatagattcc catccccatc gaacacacac caaaaaccat cgtcagctgt
      721 gctttaaaat gttcacttaa tttatttcct actaatccgc caattagtcc gccaaacaaa
      781 acggaaccta cattgatcaa aactccagtt ggcatcaaaa aactcctctc tttttctacg
      841 aagcgaaaaa aatcagaaaa atttctgtaa ttattgcttt cgctgcaaga aagattgtaa
      901 cagacagaga agattacacc aattcatttt ttgtgttaaa gtattcttta ttggaataag
      961 taggaggaat atgatggata tccataaatt cgaagttttt cttgatttag ccgaaacaat
     1021 gaatttcacc aaaactgctg atcgacaatt caccacccaa ggaaatattt ctaaacagat
     1081 tctttcgtta gaaacagaat tagacgtcaa attattcgaa cgcgcccata gaaaaatcga
     1141 attgaccgaa gctggaagct tattgctccc atatgtaaaa aatgtcgtag aacaatacca
     1201 tgctatgcaa gaagtgcttg aaacctatac gaaagacaac aatctttcat taaatatctt
     1261 aacgataccg acaatgatca attataaagg tttttccaaa atcaccgagt ttttgaaacg
     1321 ccatcctgaa tttacagtcc agttgaaaga agtggaaagt gtcgaattaa cttacaatga
     1381 cgccaatgaa catagtgaca cgatccactt tgctagaagt ttccaggccc caccaagttc
     1441 gattgagtgg ttgcctactg aaacagacga ttttgtagca gttctaccta aaaatcatcc
     1501 tttagctagt aaaaagaaat tggatctaag cgaactgaaa aaggagaact ttttgctcct
     1561 tggccccact acgaacttgt atcaacctgt actagatctg tgtcgcgaag ctggatttga
     1621 accaaaaatc tcttataaag gagcaagaat cgacttgatc atcaatatga ttgcaaatga
     1681 catggggatt gctattgtta tggaaaaaac agtcaagaat ctattaaaag aaaatactgt
     1741 gattctccct atttctccaa caacagagag ttatcttgca tttactcgta aagtcggtga
     1801 gcattctttg gcttcagata cattttggga gtatttaaat caagaagaaa actaagccct
     1861 acattttctg tccatctatc cacccaaaaa aagagagcga cgcatgactt tcgctctctt
     1921 tcttgcatca tgtccttgtt tcaatgacac ctctaacatt tctcgtagtt caataagaaa
     1981 gcagcgattt ttatgccaaa agaaccgtaa gaacatttcc ctaatcacaa aaaaacgact
     2041 aaaaacccct aaaaaccttc aatagaattt ttaggggttt tattcttttt cctattgttt
     2101 acttaaccgt atcattgctg tcgttcctac tccttcgata ctgctataat caatacgtcc
     2161 atcatgcagt tctagaattt ttttaaccag tgaaagtcct agcccattcc cagaaatttg
     2221 acgacttgta tctccttgat aaaatttttc aaacattcga tctctggtct cttcattcat
     2281 tcctacacct gaatcggtca cttccagtac aatctctgtg gctgtctcaa atagtttgat
     2341 atgaatctgt ccattgattt gattgtactt tatggcgtta tccatgatat tcagccacac
     2401 ttggtacaaa aactcttcat tgcctgtgta atttactgca gcaagttcga tatccaattc
     2461 tagctgttct ttctcccatt taggctgaaa aaaaacgatt acttctctga tttgttcatc
     2521 taatcgatac tctttctttt ccagttggat acgctggttt tccagtttgt tcaattttaa
     2581 gatgttctct gttaattgtg aaagctgcgt gatactttcg ataatccgat ataaaaatat
     2641 ttggcgttct tcgtcagaga gatttggggc ttgtaacaat tgaacatatc cttggattgt
     2701 tgctagtggt gttttgaatt catgggatac actggacaca aagtcattcc tcagtgtctc
     2761 gatactattg agttcttgga ccataacgtt gaaatcttta tacagttgct gtacttctgc
     2821 tactttctgc tgttcatcca ttcgaatcga aaaatctccc gttgctacca gactcatatt
     2881 tgtccctagc gcactgattg gatgcaggat acgccgccca acaaaaatcg atattcctgt
     2941 acccacgacc atactgaacc ccgcaaagac gaataacggg tataacgttg gttttccagc
     3001 cgattcagca ggcgagatag aatgtcgaga caataagaaa ataaaaccga gaaaacacag
     3061 cagtgtcact aagatcgaaa tgaagacaat actagcaaaa tagatccata attgtgaaaa
     3121 tagctttttt ttcatcttta aataaccgcc ttatagccta gtccccgtac tgtgatgatt
     3181 tgaaaatctt ccgtcttttc aaaacgggtc cttaaacgtt tgatatgtac atcgatcgtt
     3241 cgttcttctg tattcgtatc taatccccaa atatcatcca tcaactgttg gcgagtgaag
     3301 atcttgttgg gataagacag taatttatac agaagcagaa attctttttg aggcagaatc
     3361 aacgaatgct gatcttgtaa cactgtataa gcatctagct ctaattttgt ttgtccgatt
     3421 atcaagctac tgctgtaatt gatctttgaa cgtcgaagca atgcttccac acgtaggatc
     3481 atctcattga catcgatggg ttttgtcata taatcatctg ctcctaattt gaacccgcgt
     3541 ttcttatcct caaaatcggc ctttgccgta atcattagaa caggtgtgtc aaatctggtg
     3601 ctacgtaatg agtctagcaa gtcatagcca tccattttcg gcatcatgat atctgtaatg
     3661 attaattcta tacgtgtcgc ttctagtttt ttcagcgcat cctctccatt caatgcggtg
     3721 atgactgaaa agcccactcg ttccaacaca gaacggtaaa gcaactgtaa ttgcggatca
     3781 tcttctacta ctaaaatacg attcattgat ctccctctgt tttttgaaaa atttctagat
     3841 tttttcttgt agttcatctt cagttaacat ttatttcgta tactacgatc attgttctga
     3901 tatacttatt agtataccga atatttcaat acgggagaag ggtgattatg cttcaattaa
     3961 aagatataaa aaaatattat aaagtcggcg aaaccaccac caaagcattg gatggtgtgt
     4021 ctgtcgcttt cagacaaaaa gaattcgtag cgattttagg tcctagtggt tctggtaaaa
     4081 cgactatgct gaatgtgatt ggtggactag ataattatga ttccggtgat atggtaatca
     4141 acggaaagtc tactaaagac tttaaggaca gtgactggga tgcctaccga aacaactcta
     4201 tcggtttcat ttttcaaagt tacaacttga ttggtcatct aggcattatt gaaaacgttg
     4261 aacttgggat gacattaagt ggtgtttcta aagacgaaaa acgaaaacgg gcagaagatg
     4321 cattgcaccg agtcggcttg acaaatcata tgcataaaaa gccaaatcag ttatctggtg
     4381 gacaaatgca gcgagtggcc atcgctcgag cactagccaa tgacccagat atccttttgt
     4441 gcgatgaacc aaccggagcg ttagatacag aaacaagtat ccagatcatg gaattgatcc
     4501 aagagttatc gaaagaaaaa ttagtgatca tggtcacaca taatcctgaa ctggcccatc
     4561 aatacgcaga tcgaatcatt gaattttctg atggtaagat cttaacagat tcccatcctc
     4621 acatcgaacg accaaaagat gatcaattta acttacgcag aacaaagatg agtttttgga
     4681 cagcgttaaa gctttccttc aacaatatcc gaacaaaaaa aggacgtact ttcttgacct
     4741 cctttgcttc cagtattggg atcatcggaa tcgcgatcgt tctgtccctt tcttctggat
     4801 tccaaaaaca aatcgataac acgcaagctg aaacgatggc gaaatttccg attactattt
     4861 ctaaggtaac gactaaccag actcgtgacg atgccggatt gggtgcaagc aaagcggatt
     4921 atccagacag caaaacgatt actgcaaaag tcagcgaaga agataaagca cagcacacga
     4981 ataagattga tcaaacatac gtcgattacg taacagacat cgatcctaac ttaagtaata
     5041 atatcggatt tacccgtact acaggaatca atcttttacg tgatgtaaat ggcaaagtac
     5101 agcctgtcag cttctcgaat caaaatcctg atacggaatc tctttcttta tctagtacga
     5161 tgtcagcaat gacaggtgtg ggagtgtcaa gtttccctac acagcttgac actagcaaag
     5221 aaaacttttt aaaagacaat tattctttac ttgccggaag ttatccagcc tcagcaacag
     5281 atgtcgtgct gatcgtagac ggcaataata atactaatat caacgcattg aagaatcttg
     5341 gatttgatgt gaaagaagac gaaaaacttg attttgatga tatcgttggt acaacattca
     5401 aattagtaaa caacaatact tactatacaa aacttccaac tgggaatttt atccctaata
     5461 cagattatga tgcgatgtat cagaatgctt ctgatgaatt gaagatctcg gggatcctac
     5521 gcgtgaaatc ttcttctaca atgaatctct tatctccagg aatcgcttat agcgatcaat
     5581 taacgactca aatcgtcaac gaaaacaaag aatcagaaat cgtcaaagct caaagagaca
     5641 gtggtgtaaa tgtcttaact actgaaaaag ttgacgaaaa tgccaaacaa acattgctta
     5701 gttatttagg tggagacagc ttgccttcaa gtatcatgat ttatccgaac aattttgaag
     5761 acaaagaaaa gatcttggat tatctagatg attacaataa aggcaaatca gatgaagata
     5821 aaatcattta tactgattta gctggaacga tgactgaact gactggcgga ttgatggatg
     5881 ctatcactta tgtccttatt gcttttgcag ggatctcttt agtaacaagt atgatcatga
     5941 tcagtatcat tacttataca tctgtgattg aacggacaaa agaaatcggt gtactaaaag
     6001 ctttaggtgc acgtaaaaaa gacatcactc gcgtatttga cgcagaaacg tgtattttag
     6061 gtatttcttc tgggatatta ggtgtgttta ttgcatggct agctacgttc cctatcaata
     6121 gcatcttata caacatgacg gatctaaaaa atgttgctca actaaatcct gtccatgcga
     6181 ttattctagt gatcgtctct actatattga cgatgcttgg cggtcatctt cctgcacgaa
     6241 tggcagcgaa aaaagacgcc gctatcgctt tgcgtgcaga ataaggttgt gacaaccaca
     6301 tttagatttg agtcataaga gcgctgaaat agtagaaaac agcaagaaac gaggaagtaa
     6361 tatgttcctt gtttcgcgct gtttttttta attatttttt ataattctgg tatacttttc
     6421 tttcctttgt accacaactc ttctggcgta tacctgtctt ttccatttat gaatactgtt
     6481 ccatctgctt caatcgttac ttcagtccca atccacatat tacgagaaag tgcgagaaga
     6541 ttttttcttt ttcctcttcc tttttttcca aattgcaagg catcggttat ctcttctgtc
     6601 caatgagatt tcacgtattt tacttctccg ttttcaaatg taactttcaa aatattcgct
     6661 atattttcca cttttactgc tttaggaaat gactcctcca tttttttcac tttccttcct
     6721 ccaacactaa gtatgcgctt tcttttagtg ataagtatat catagtttat tctattatca
     6781 aaaaaacgca tccattccat tgtagaatac tttaccgaac aagttgaatt gttttcctct
     6841 ttttcacctt ttactataga taggtaaaga caactaagga ggaatctctc atgacagatc
     6901 aaccgtatga tttaagaaaa gtcatcgaag aactaaaaac acttccaggt caatatcacg
     6961 aaacagatat tgaaatcgat cctgaagcag aattatctgg tgtttaccgt tatatcggtg
     7021 ctggcggtac agtaaaacgt ccgactcaag aaggaccagc tatgactttc aacaatatca
     7081 aaggtttccc cggcacacgt gtgaacatcg gaacaatggc tagtcgtaaa cgtgtaggtg
     7141 aaattttgca ccatgattat cgaacacttg gtcgtttctt gaaagacgct gtagaaaatc
     7201 caatcaaacc tgtgaaagta gacaaagcag acgctccagc acaagaagtc gttcatctag
     7261 ctaccgatgc agacttcgat attcgcaaca ttttgcctgc acctacaaat actcctgaag
     7321 atgctggtcc atacatcaca atgggtgtag tcttaggatc agatccagaa aaaacaatga
     7381 gcgatgtaac gatccatcgt atggttctag aagataaaga tacgatcggt atgtacatca
     7441 tgcctggcgg acgccacatt ggtcacttcc aaaaacaatt cgaagccttg aataaaccaa
     7501 tgcctatcac tatcaatatt ggtttagatc cagcaattgc cattggcacg actttcgaac
     7561 cacctaccac tccacttggc tacaatgaac tatggtgtgc aggagctttg cgtaacgaac
     7621 ctattcaaat cgttgatggt atagcagtcg atgaagtggg tattgctcgt gctgaattca
     7681 tcattgaagc agaaatcatg ccaaatgaaa caatgcaaga agatatcaac acgaactcag
     7741 gaaaagcaat gccagaattt cctggctata ctggggatgc taatccagct gtacaagtag
     7801 tgaaagtcaa agcagttact caccgaaaag acaatccaat catgcaaaca acgattggtc
     7861 ccagtgaaga acacgtatct atggccggta ttcctacaga agcaagtatt cttgccttgg
     7921 tcgataaagc tattccagga aaagtagtaa atgtctacaa tcctcctgca ggtggtggta
     7981 aattgatgac catcatgcaa atccgtaaag aaaacgaagc ggatgaaggt attcagcgtc
     8041 aagcagctat tcttgcttta tcagccttca aagaattgaa gacagtcatt ttagtcgatg
     8101 aagatgttga tattttcgac atgaacgatg tagtttggac tttcaacact cgtttccaag
     8161 gagataaaga tatcatcgtg atcccaggaa tgaggaacca tccattggac ccatcagaat
     8221 taccagaata taatcctggc tctattcgtt tccgtgggat aagtgcaaaa actatctttg
     8281 acggtacagt tcctttcgat atgaaagaac attttgtacg tgcaaaattc caagaagtag
     8341 aagattggga aaaatatttg aaataatgaa tgatctgcaa ctgttttgac atcataaaaa
     8401 ggagtgcgga aaatggaaca attggcacaa cggaagaaaa aaatcgtgat tggtgtaagt
     8461 ggcgcttcag gcaccattta tgccatcaac ctagtgaaga aactaaaaga atatccgatg
     8521 atagaaacac acggaatcat cagtccatgg gcaaaacaga acttaaagct ggaatccgac
     8581 ttatcactaa ctgaattcga aagcctgttt gattaccatt attcaaacaa agatttaggt
     8641 gctgctgtcg ccagcggatc atttttgacc gatggaatga tcatcgtacc ggcaagtatg
     8701 aaaacagtcg caggtatctc tgttggttta ggcgacaacc tgattgcacg tgctgctgat
     8761 gtaacaatga aggagcaacg aaagttagtc attgttcctc gtgaaacgcc attaaatgcc
     8821 attcacttgg aaaacttgac taaattagca cgactaggtg tgcaaatcat accaccaatt
     8881 cctgcttttt ataatcagcc tcaaacgatc caagatcttg ttaatcacca tacaatgaaa
     8941 cttttggatc aatttgaaat tgtaaatgag cattcaaaac gttggcaggg tgattaatat
     9001 ggaaaaagaa acgatagttg aagtaaccaa actaggtttc acgatccaag ctgctgttaa
     9061 agtaatcggt aaagacttat tgatcacttt aacaggcgga gatacaccac atatcggaac
     9121 agtcactaca ttttctcgtg atacagagat gcaaactgta cgtttcccta gccacgatgg
     9181 tcggttccat aaagacgatg tcttagccga aaaaatagct gtgatcatca aaactgccgt
     9241 tccgggaagt tgtacgatca cttccggtat ccatgttgac catatctcca atgcacaaat
     9301 caacgcctct tttccgatgt cagaagagtt aggttacaag attttagatt ggttggaaaa
     9361 aatagatttc catatcaaag aaccagttta ttataaagat ggtgaaaaac cattatgaaa
     9421 gaagaggttg tgaaatcagc gttatgacct gagtatgaga gaaaaaaatg gaaaaatagc
     9481 ttctcatatt ttgccgtttt tgagctaata ccgagggaca gctgattgaa cgccgtttat
     9541 caagaggttg tgataaagcc gcttcgtttc gagtagtaag aaaaattttg taaacatggg
     9601 tttccacatt tttgcataaa tttgacttac taccgagaaa ctggcttttg aacaccgttt
     9661 atcaagaggt tgtgacacgt cttttgtcac aacctctttt atttttgctt cactgtaact
     9721 gctacaatat caccgatttc ttttccgatg tttttacgaa tatcttttcg tagtccaagg
     9781 atgtagcaga ctgaaccatc tggatttttc acgcccatat tcacgatact gccttcatac
     9841 ggcacattgt caaaagttgc ttggacttta actcttccag caccgaattc ttttcttata
     9901 tcatacggaa aaataatata tgccccgccc ttgccgactt cggaagcata gatttttgat
     9961 tcaaaagaat aaatttccct cattttattt ctcctatttt ttacatctag tatagcatag
    10021 caaaaaaaag aaggaactgt gacaaaagtc ttgtcgcagt tccttttttc gaataaacgg
    10081 tgttctcctg ccaactcctc ggtcacaagt cacaatagtc aacaaacatg agaagctgtt
    10141 ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacaacct cttccttaaa
    10201 cggtgttcaa aagcttgcat ctacggcact aagcccaatc agatgaaaaa tatggagtgc
    10261 tatttttatc tgattgttct tactgcttga tgcagagcag ctttttcaca acctcctaca
    10321 tggtgttcaa aatattattc gccttttgct tctttcacta attttgcgaa tgcttgttgt
    10381 tggaattcag cagctaaggc tttcgtacgt tgttcttgct ctgctttgct catttttgga
    10441 ttttcagtca ttgcttttac cataccgtct tgaacataag cttttgcttc actttccatc
    10501 gtactttgct gtttcttcac taattcagct aacttggctg cttggtccgt acttaattct
    10561 acccatgtct ctgggatttt gaatgtattc actcggctgt catattcgtg attgttatca
    10621 gcaatattga accagaattt atacatgtcg tttttgtatt cccaataagt agttttcgtc
    10681 actttttcag catcgccgtc tgtcttttct actttatttg tttcgttatc cgtaccagtt
    10741 gcttttggtt tttcttgttt ttcatttgtt cggtaaagat acactttttc ttttccattc
    10801 cctaaagctt tatacaaaag catatctgct ccttgggaat cagcactgga aacaattttt
    10861 tctgtttttg tttccgttac tttttccatt ccaaagtgat tggacaaatt agcgacgatc
    10921 aaacttagtg aagcgacgaa gattagtccg aaaatcaaag ataaaaaggt ttgccatgtt
    10981 tttttagcga acacgttcgt taaagcaaaa gcaagcaagc tgacaatcaa aactccaata
    11041 atcatgcatt atcccccgct ttcattagaa caggttcttt tttcttcaat aaaaaggcaa
    11101 taataaagcc aagaatccca aagatgatcg ctacgataaa cgcggcatgg taacctgaca
    11161 gtgtggcatt gattgcctga tctttataag ctaatggcgt attatccaat acagatttgc
    11221 ttgggatatt tgcttttgtc acattcgtca acacactgat caaaatagct gtaccaatcg
    11281 agcttgcgat ttgacggaat gtattattta ccgctgtccc atgactgatc aagttatttg
    11341 gcaatgcatt cataccagaa gtcgttaccg gcatcatgac catggagatc ccaaacatac
    11401 ggatcgcata aaggatcacg atatataaaa ctggagtcgt ttttgtcaag aaagcaaatg
    11461 gtgcagttgc cgctgtcaaa atgaacatcc ctgtgatggc taaacgtttc gcgccgtatt
    11521 tatcgaaaat cgcacccgtc acaggcatca tagcccccat gatcaatgca cccggcaaca
    11581 acatcaagcc agaatggaat gctgattctc cacgaatatt ttgaatatac aatggtacga
    11641 ccatttctgc ccctaccatc gccatatttg ttacaccagc taaaacagct gcaatcgtga
    11701 atgtagatga tttgaataca cgaagttcca agaatggatg ttccaaatgt aattgacgcc
    11761 atgagaacaa ggcgatgaaa atcacaccaa caactaagaa accaattact ttcaagcttc
    11821 cccagccgtc atctcctaca ctagaaaatc cgtaaagtaa actaccgaat ccaatcgttg
    11881 ataaaatagc ggataaaata tcaatgctag gatttgacaa tggaataaca cttttcatta
    11941 ggaagaatgc taaaaccaag acaagaacaa cgattggcag taccatacca aacaagtcac
    12001 gccaatggta gttatcaatg atataaccag ataatgtagg gcctaaagca ggagcaagtc
    12061 caataacgat ccctgacatc cccattgctg atcctctttt ttctggcggg aaaatagata
    12121 gcatgatatt ttgcaataat ggcatcgata ctcccacacc gcaagcttgg atcaaacgac
    12181 cgattagtaa tgtactaaaa ttaggtgctg taaagcaagt aatcgttccg atcaaaaaga
    12241 tcgtcatggc agaaagatat aaatttcttg aactgaattt attgatcagc catgctgtta
    12301 ttgggatcat gatcccgttg actagcaaga aaccagtcgt taaccattgc acatcagacg
    12361 ctgaaatatc gaaagctttc atcaatgtag gaaaagctgt cgtcaataag gtctgattca
    12421 aaacggtaca aaatgtacca attaataaga caacaactaa aagtgtccga ttatatggtt
    12481 ttccgtaaat atctacgggt tggttgttac tcatatttct ccttctttct gaattttttc
    12541 tgaaactact agaatacttt agtcctaaat tcgctatttg tcaacctact tgacacataa
    12601 gttaatacgg atataattaa gggaagcaag aggacaaaag gagtgacaaa aatggttggg
    12661 gcttccctta aacgtttgtt ggaaaatgat caattggttg ttggcatcag tgaattaagt
    12721 gatatcgtag gtgtgtctcc acgtcaatta aggtattggg aacaaaaggg attcattcgt
    12781 tcgatcgaag ctgatgcgaa ttgtcctaga aaatatcgtt tacctacagt cataaaagta
    12841 gaactgatca aaaaatattt agatgacgga tttactttgt caaaagctgc agaaaaggca
    12901 gaaatccgcc aaaagaaaat gcaccatgtc cgtaaagttt tttcaaaagc aatcaaagat
    12961 atcgaattga tcgacgaacg ctatacgatt ttacaaatcg gtaacttcaa agaagagaat
    13021 gctaagatgt acatcattca tgacaacgag acagacgaat taacttacaa gctccttcct
    13081 gtcgatgaaa aagctgacta cgaacagttg ttaaaagaac tctaaacgcc actaatctcc
    13141 taagtaccaa gctttttaat aagtagatgc tacataaata cttgaaaaaa ggcctgtttg
    13201 aacataagaa tatccgaacg gtgatctgcg tttctttcat atagaaagga attctccatc
    13261 actgttcgga tattttttta tttttttcgg ttactttctt ctaatacatc tgttagccaa
    13321 tttgcatatt ctgtttcccg attgatcgca tgcgctaaaa tcaggtagtg tccgtaattt
    13381 tcatgttttt ctttttcagt aggaaagatc gttttttttc gctctaatag atgaagtcgt
    13441 ttttcttcat gaaggtcacg ctgctgctga ataatttcta ctaatgccgg atcattcacg
    13501 tctttgacga aatacaattt cagaacaaat tcgtctcgat taaccggtag ttcattggtg
    13561 ggtgtatgaa tccactctgc caattgttcc ttcccttctt ctgttaattc ataaactttc
    13621 ttttccaatt tttcacctgt gatctctact tgatgctgaa tgattccttg ctcttccatt
    13681 tttgcaagtt caggataaat ctgactatgt tttgcttgcc aaaattctcc tatttcattt
    13741 ttgaacgatt gattcaattc atatcctgtc atcttcttct tacttaataa ccctaacaaa
    13801 atatatcgaa gtgtattttt tcttgccatc agttttccct ccttgacaaa tctttaaatc
    13861 cattctatca taaatgagta atcgaaaaca tgtagtttat tacatgtttt aatttataag
    13921 gagatgctta aacatgaaag aatttaaaga attggaagat tttattggaa ctcactttat
    13981 ctatacgtat gacaatggtt gggaatatga atggtatgcc aaaaatgacc atactgttga
    14041 ttaccgtatc catggcggaa tggtcaaagg ccgttgggta aaagaccaag aagctgatat
    14101 cgttaaattg acggacggcg tattcaaaat cacttggaca gagccaacag ggacagacgt
    14161 ggcattggac tttatgccaa atgaaaagaa acttcacgga atgatctttt tccctaaatg
    14221 ggttgaagaa catccagaaa tcactgttac tttccaaaac gaacatatcg cagaaatgga
    14281 agcagctcgt gaaaaatatg aaacttatcc aaaattactt gttcctgaat ttgctacgat
    14341 cacttatatg ggtgatgcag gtatagacaa tgaagatgtt attagcgaag aaccatacga
    14401 tggaatgcca gatgatatcc gtagcggaaa atacttcgat gaaaattata aacgagtaaa
    14461 ataaggttgt gaaagagagg ttgtgaaatc agcgttctga cctgagtatt agagaaagaa
    14521 acggaaaaat agttcctcat gttttgccgt tttttgagct aataccgagg gtcagctgat
    14581 tgaacaccgt ttatcaagag gttgtgaaat cagcgttatg ctttgagtaa taagaacgct
    14641 agttagaaaa tagcttttca tattttctag gctagtgggc ttattaccga gaagcagccg
    14701 tttgaacacc gtttatcaag aggttgtgaa atcagcgttt tgacctgagt attagagaaa
    14761 caaatggaaa aatagttcct catattttgc cgttttttga gctaataccg agggtcagct
    14821 gattgaacac cgtttatcaa agattgtaac aactgcatat tcacacagtt aaattagtga
    14881 cttcaaaatg aaataatgct cccagcaaaa aattaggctg ggagcattat ttcgttattc
    14941 tgttcatcaa aaatcagcgt tttgattcca tttcatcaca agttcctcct catttactgc
    15001 ttcatctttt tgctgtgttt gaattgtaaa accttgtgaa agatagaaat gaatagctga
    15061 ttgatttttt tgatagactg tcaaagtcag tgttttattg gtttctttta tttttttcat
    15121 tagttgcgta ccgattcttt gttgccggta ttcccttttc acaaagattc ctgcgataaa
    15181 ctgatcaatc accccagcaa aaccaataat ttcttgatct atttctgcta agtaaatcac
    15241 tgcatctggt aaagctttcc taacagattc tacttgattc tcccaataag ttgatggaat
    15301 gaattcatga gaatccaagt tagcatgcag ccaaatcgat agaacagatt ccagttcttt
    15361 ggcagacaat tttgttgttg tgcggatcat tttttctctc cttacttatt cattgatttt
    15421 aaaataaaaa ccattttttt gaagctcttt tacaagtttt cgcccctcaa aaaagtatat
    15481 cttctcttgt tttacttttc taataccaaa agaaacagca actttaggat catcagctga
    15541 aacgattcga taagttccac caggcaattg atccagcttg actttcccat gatggtcgct
    15601 ttgtactgaa aacggcacac catttctagt aagtgttttt ttcttatgtt tcaaaatgaa
    15661 tgaactacca tttaccggtt tccctgacga atcactaata ataaatgaaa aatcaagcac
    15721 ttttttactt aatctatact gatagattag tcgtccgata aacactaaca gtatgactac
    15781 agctattatc gttcctgcaa gaataaacca ttgccgcatg gttctttcct gatttccttt
    15841 tccttcttct ttttttaccg attcgttata aggaatccga tgtcctgtca ctagcaaacg
    15901 atgcgtgttg atcatatacg gcgtacatgt aagcaaggta actaagtctt ctccttctga
    15961 aatcgttaga acagacgtgt catctggcag taccacttgt atatttttaa cttgataagc
    16021 caatttctcc cccaaaacag tcaaaataaa cgtgtctccg ttttctaaac gatctaattc
    16081 ggtaaataac cgtcgattag gtaaaccgct gtgagcagag atcactgaat gcgtcgaatt
    16141 tccaccaagc gggaaagagg ttccttgcaa gacgcctgct ccattttcaa ggatcctacc
    16201 attgagccga tcataaagtg aaatatttaa ttggatctta ggtatattga tacttccgat
    16261 cagccattcc tctaattggc tggatgttgt actttcattt ctgttctttt cattgaaagg
    16321 atcagtacca ggattgaatc cttggtcagt taatcgttgg tttttctcct ccattttctt
    16381 tcgctgcact tcaatttcag ctgcggtccg tttttgtact tgttcgatac gtttttgatc
    16441 aataaaacta tttaaagaat ccacataaaa tggatataaa gcgatcagta accctacgac
    16501 gatacatatt aataaaaaca aacgaaacaa aagtcgacga gactgttttt gcttactctg
    16561 cttcacatgt gtcctccctt gcaagttttt ttgttctttc ttttattata tgggttcagg
    16621 aaaaaaatag aaaatgaaag ccgatgaggt tgtgaaaaag ctgtcctgca tcaagtaata
    16681 agtacagccc aaaaaaatag ctactcatat tttttgttgg gttgggctta ttaccgcaga
    16741 tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa actgcgttat gctttgagta
    16801 ataagagcgc tagtcagaaa atagcttctc atattttcta ggctagtgag cttattaccg
    16861 agaagcagct gattgaacac cgtttaccga gaggtcatga aaaagctact ttgtttcgag
    16921 tattaagaaa aattctgttt caaaacaagg agatgcaaca aaacttatgt cgcatcctct
    16981 ctccttagaa ctttcactta tgtttttctt gtaaaattca taaatatttt catgaatttt
    17041 ctaatttctt gcctacctct cgcataggat atatgatgaa tttatctaaa gtttgcaaag
    17101 gagttcgaca attgaaaaaa tatggcccat ttttagcaat ttttttcatt gctttggcat
    17161 ttctcatgcc tcatttattc acacataaac taattttggg tgctgatgcc ccattccatt
    17221 acaatcggtt ctatgatgcc gcgcaacaaa ttaaatatgg ggatttccat tattttcctt
    17281 ccctttatgg attccagcaa agtggcagga tcgtgaatgc cgtttacggc ccatttttcg
    17341 cttatttaca aggattgatc gtgttgcttg caggtagctg gtttcattat cagattcttt
    17401 caaatttcat tctttatctt atttcaggat tttctatgct gattttactg agatatgtca
    17461 atgttcgaaa ttgggtaagt gtaccagtag ccgttttatt catgagtacg tattctattc
    17521 aatactggtc tatcaaccaa ggattcacta gctgggggac ttgtttttat cctttatgta
    17581 tgatccctat tgctgatttc gtccttcata aaaagttccc tgttatcaaa gtcgctgtct
    17641 cagtgggact tatgacacag atccatttac tgtcttcgat catgttagct ttgatgtata
    17701 tcccttttta tctttattac ttttttaacc agactgaaaa aggaaaagca ctgcttcaac
    17761 tgattaagtc ggtcttgctt tattttgttc tgacagcaaa tatttgggca ggattagcta
    17821 ttgtttacag tgggaataag attcttcctc cctttgttaa cagagaaatg agtcaaaagg
    17881 cgatagatct ggatggcagt tattggctcc attaccctaa ggcctttcct atattgatat
    17941 tatcaggttt tttgcttttt attttctttc aacaaaagaa caccttattc caaaaaatcg
    18001 tgttgtacac aagtcttgtt ttcttttttc tttctactag cttgattcca tggacgacat
    18061 tgatcgaaaa aaatgtgcct tttattagtt tgatccagtt tccatttcga tttttcgtgc
    18121 catttacgct attgcttcta ctttaccttg ccctttcact gaaccagtgg tctgaacaaa
    18181 agtggttccg tcttttaagt atatttggca ttcttatttt ttcagcacag acgatccaga
    18241 acttatacca gcatctggaa aagtgggaaa atgaaacatt tgtcagtcga catacttatc
    18301 tattcgatac gccggaagaa gcaaggaaaa cattcttcag caaagatatg tctaccagct
    18361 tgttcgtttt ccaaaaaacc acacctgact atctgccaat ctataaggag acaaaagaca
    18421 ataaatacga ccggtataac gaagaaatac tagaaaacga aacaactttt ataaaaacac
    18481 atccaggcgg tcttttgaca atcaagtgga aaaacactgg ggacaaaact gttcatatcc
    18541 ctgttatcgt ctacgatcgg actgtgctac agcaaaatgg aaaaacattg actgattatg
    18601 aagtaacaga tatcgggacc ccaatcgtga agcagcaaaa aggaatcaac gagctcacgc
    18661 ttcattatca aacgccaatc tatttctatt tcattctttc tcttactttg attggctggt
    18721 ttaccttgct atgtttattt atttatcata gatacaagct tttacgtgct taggcaaaag
    18781 ggataaatac atccgatcaa aaaactctcc aaacggtata atcgtttgga gagttttatt
    18841 ttaatcagtt tttttatgtt atatgttata ttttttattc gttcacatct aattcgattt
    18901 ccaccccgta gtgatcactg atgattggac tatcgatccc atcaaaaatc cgttgatatt
    18961 ttttaacctg cattccttca gggacgaaaa taaaatctat acgcaatttt tctgtatttt
    19021 gttcccaacc atctattttc ttttccacag tagctgcttt tcctttttca ttcgcgacaa
    19081 catacgaatc ttgaagaaga aggcaacttt ctcttacagt ataataacct tgagagtcta
    19141 ctggattatt gaaatcacct aggaaaaaaa gtggtacgcg agtttccaaa aagtagtttt
    19201 ccaagttttt ccactcaaaa taaaagccac tttttttatc tgtccaccaa gaaaagtggc
    19261 agctcgagac aaaaatgtcc tgatcatcta tctttgtttt cccaatcaag atcttgcgtg
    19321 tctgataatc ttctttcttt tgaaattggg agacggtaag gacctgactt tccaatgttt
    19381 ttttcgtaag cagtgcattt ccttcttcat atttatcata accgatgtgg ctcatttccc
    19441 aactccaaaa atactctttt ccgtgttcct ttaaaagctg aactaaacgg taggcaaaat
    19501 tatctttacg gataggtact tgattcttta ccggacagaa cttcattagc ttcgcaggtt
    19561 ctacttcttc agattccagt aattggttga tttcttgtaa agcaatcact tcataatctt
    19621 ctaacaggat cttatctgct aattcttgca attttttctc tggttctggc tcaagccagc
    19681 tatgcgtgtt gagtgtcatg aatttcattg gttttctcct tgtcttcatt tcgaaacaaa
    19741 agggttgtga caaaattttt gtcacaacct ctccagcaaa tagtgttctc ctgctaactc
    19801 ctcagtaata agctcaatgg accaggagct attttttgtt tggctgtttt tatcacttgg
    19861 tgcagggcag ctttttcacg acctctccac taaacagtat tcaaactgct gcttctcggt
    19921 aataagccca accaaacgaa aaataggagg agctattttt gtttggctgt tcttatgact
    19981 tgatgcagga cagctttttc acaacctctc cttacatttt aatggagccg atcactttac
    20041 cagctgtcac tgtacctaat tgcttgatca ctacttcttc tactttatcg tttgtaaata
    20101 cgatcatgat cgttggatct ttttcttcgt tttggatcac atcaagatcc atctcagcta
    20161 atttcgttcc tgttcctact tcttggcctt ctttgacagc aatctcaaaa gctggctgat
    20221 tcatttggac tgtatcgatt cccatatgga tcaaaacttc gataccatct ttcgtttcca
    20281 gcccaattgc atgtttgcta gggaaaatac ttgtcacttt tccagcgatc ggcgcaacaa
    20341 cttctcttga tgttggaata acagcaaagc cgtcacccat cattttttgt gagaataccg
    20401 gatcttttac ttcagataaa ggaatcactt ctccagaagc tgcggaaact agttcttgtt
    20461 ctatacctaa gtattgctta ctacctgctt gtccatttcc tgtagaaacc atttctggct
    20521 ctggaatctc tgctcctgat tgcagcagat cttcgatatc tgatttcaag acatctgctt
    20581 ttggtccata aactgcttgg actccattgt ctttgacgat caatcccatc gcccctgctc
    20641 gtttccaggc ttcttcagtc cctacttttt ctcgatcatt tacacttacg cgtaaccgag
    20701 tcatacaagc atcgacatct ttgatatttt cttttcctcc cagcaaatga atgatctgga
    20761 ccacttgctg atctgctgtt cctgttccgc tgttagatac aacggtttct tcactgtcat
    20821 tgtcgtaatt tccgttacgt cccggtgtag caaagttgaa ttttttgatc aagaaatttg
    20881 ctaagaaata cgtaacaaca ccaaaagcga tcacacacaa cacgaagttg ataagatctc
    20941 cacctagacc cgctttgata gctaacggtg tccgagtcag caattcgata ttcccaaatg
    21001 aatggacccg caatggcaag atatctgcca tcgcaaatgc agccccttgg atcactgaat
    21061 aaatgacata taatggcaca gcagcaaaca taaacatgaa ctccaatggt tctgttacac
    21121 ctgttagaaa gacagccaac gctgctgaaa aatacattga tttatatctt gctttcttat
    21181 caggatcgac attacgatac atcgcaaaag ccattcccat caagatacct gaagaaccaa
    21241 tcatttgtcc tactttgaaa cgagccggcg tccagttatg caacacaaac tcatattgcg
    21301 acatatcccc tgcaccttta agattcacta aatcagtggc ccaagccaac cataacggat
    21361 cttgcccaaa tacttgtgtc cccgcctgtg cgcctgataa aatctcatac gatccgccaa
    21421 gctgtgtata gttgatagga atcgtcagca tatgatgcaa tccaaatggc aatagtaaac
    21481 gttctaatgt tccaaataaa aacggtgcta gaatcggtgc gctttcttgt gattctgcaa
    21541 tccataaacc aaaattattg attccagcct gtacgtttgg ccacaccaca gcaagaatca
    21601 atgccacgat tgttgaccat aagataacaa caaacggaac aaagcgcttt ccattgaaaa
    21661 atgacaaagc atccggcaat tttctataat tatagtactt attataagcc attgccccga
    21721 caaatccagc gatgatccct acgaatactc ccatattcaa agctggtgct tccaatacac
    21781 ttgtaaaaaa tcctttaacc atgatcttcg taccgaacag agtgtgggta aatgcatctt
    21841 cattcgcaag catgtcactt gtgacaccaa agatagcccc agtgatccga ttgattaaaa
    21901 taaatgagat cccagctgca aatgctccgc ctgctctttc ttttgcccaa cttcctccta
    21961 ttgctaaagc aaataaaata tgaagattac caataattgc ccaaccaata ttttcaatta
    22021 ctccgcccgt tgtgacaagc ggcgtccaat caggattgat caagggaatt gatttcccta
    22081 tactgatcat caacccagct gccggcataa ctgctaccac aaccattaaa gcctttccaa
    22141 atttttgcca aaactcaaaa ctaaatatct ttttcattcc gtttagctcc tttacgcaat
    22201 cggtttcgtt aaacgtatta tagcgtgcgt tttctttttt gtaaatgctt tattttttat
    22261 ttttctgtct ataccattct ataaaaccta taatacgtgt gttcccttct ataattttct
    22321 cctaaaaatt tattttttta agcttttttc tatgaaatat tttcttttgt ctattgactt
    22381 gtgcaatcgg ttgcgtttat tatgaacgta taaaacagct cattaggagg agaaaaatga
    22441 aacagatcaa acgactattt gctattgatc catggaaaat cagcacgaca caattagata
    22501 aaaaaaatct tcgactgcaa gaatctttaa caagtattgg aaacggttat atggggatgc
    22561 gcgggaactt tgaagaaact tactcagggg accaccatca aggtacttat ttggcgggtg
    22621 tctggtatcc cgataagaca cgtgtcggct ggtggaaaaa tggttatcca gaatactttg
    22681 gaaaagtgat caacgccttg aactttatcg cgatagatat ttcgatcaac gatcagctga
    22741 tcgacttggc ttctttggaa ccagaagact tttactggga attagatatg aaaaatggtg
    22801 tattatctcg tacattcaca atcactactt ctactaataa agtacgcatt tccttcgaac
    22861 gttttctgag cattgtcaaa aaagaatcag cttatatcta tttaactata gaaatgcttg
    22921 aaggtagcgg aacagtcaaa ctcgtatcaa aattggatgg aaatgttcaa aacgaagaca
    22981 gcaactatga tgaacatttt tggaatgaga ttgatcgtgg aactatagaa gaaatcggct
    23041 atttgacgac taaaactatt ccaaacaact ttgggattga acaatttact gttactgcag
    23101 caatgcgcca tttcgttaat ggaaaatcag tcatccctga ctatcacgaa gatactttag
    23161 cattacatgg ttgcttgtct gcagaattat ctcaaggtga gacatggtca gtggaaaaag
    23221 aaattatcgt tcttactagt agagatattc cagaagcgca gcaagcagaa aaagctgtct
    23281 cagaattaac gaaccagaca aacaattata tccaagcaaa agaagagcaa acagcggctt
    23341 gggcagaacg atggaagctt gccgatgtgg tcatcgaagg agacgaagaa gcacaacaag
    23401 gtattcgatt caatttgttc caactattct ctacttacta cggcgaagat gaacgattga
    23461 atatcggccc caaaggattc acaggagaaa aatacggcgg agccacttac tgggatacag
    23521 aagcgtatgc tgtccctctt tatcttgctt tagccaaacc tgaagtaact aagaatctgc
    23581 tgaaatatcg gcataatcaa ctgccgcaag caatccacaa cgctcagcaa caaggattaa
    23641 aaggcgcgct ttatcctatg gtcaccttca ctggtgtcga atgccataac gagtgggaaa
    23701 tcacattcga ggagattcac cgaaatggcg cgattgccta tgcaatctac aattatacga
    23761 attacaccgg cgatacggaa tatttgaaaa atgaaggact ggaagtttta gcagaaatcg
    23821 cacgcttctg ggcagaccgt gtccattttt caaaacgagc aaataaatac atgatccacg
    23881 gtgtgaccgg cccaaacgaa tatgagaaca atatcaataa caattggtat acaaatacga
    23941 tcgcagcttg ggtcttgcgt tacacgcgag aaaattattt aacttatcaa aaagaagctg
    24001 ctatcactat cagcgaagaa gaactagcca aatggaaaga tatcgcagag aacatgtact
    24061 tcccagttga cgaagaatta ggaatctttg tccagcatga cacattctta gataaagatc
    24121 tcatgcctgt atccgatatt cccacaagcg atcttcctct gaatcagcat tggtcttggg
    24181 acaaaatcct tagatcttgc ttcatcaagc aggcagatgt actacagggg atttatttct
    24241 tcaatgatac ttttacaaaa gaagaaaagc aaaaaaactt tgatttctat gaaccaatga
    24301 ctgtccatga gtcttccctt tcgccaagta tccattcgat tttagcagca gaattaggaa
    24361 tggaaagcaa agctgtagaa atgtaccagc gaacagctcg cttggatttg gataattaca
    24421 ataacgacac agaagacggc ttgcacatca cttccatgac tggtagctgg ttgtcgattg
    24481 tccaaggatt tgctcaaatg aaaactgccc atgatcagtt gagattcgca ccatttctgc
    24541 caaaaacttg gaaagcttat tctttccata tcaattatcg cggacgttta ttgttcatct
    24601 cagtcacaga tgaacaagtt actttcgagt tattagaagg ttctcctcta gaaatttacg
    24661 tctatgaaca gtcttattct ttagcggatc gtctagaaat aacgctaaga aaggaggaag
    24721 caaatgttta aaggggtatt gtttgattta gacggtgtca ttaccgatac tgctgaattc
    24781 cattatcgtg cttggcgaaa gcttggtcag gaaatcggca ttacgatcga ccacacattc
    24841 aacgaacagc taaaaggcgt cagccgagaa gactcactct ctttgctttt agcttatggc
    24901 ggaaaagaac actctttttc aaaggaagaa tttgcagaat tagcgaaaag aaaaaacgat
    24961 tattatttag agatgatcca gacgatcgaa ccaaaagatg tcttccccgg ggtcgtgcct
    25021 ttactggatt ctttaaaaga agaaaaaatc aagattgctc ttgcttctgc aagtaaaaac
    25081 gggccattcc tacttgaaaa aatgggtctt acaccttact ttgatgctat tgccgacccg
    25141 gcagaagcaa caaacggaaa acctgcgcca gatatcttcc tccttgcagc aaaagctgtc
    25201 ggactaaaag cagaagaatg tatcgggatc gaagacgcgc aagccggtat ccaagccatc
    25261 ctctcaagtg gcgctcaacc aattggcgtg ggacgtgctg aagaccttgg agaggagatc
    25321 ccgcttgtcc ctgatacgac ttttcttacg atcgattact tgaaagaaaa gtggcatgac
    25381 catggataat ccagagatct ccgtcgtacc agttccagag actgaaataa agaaaatcac
    25441 attgaagaac gactacttag aagtcgttct tctcaattac ggtgcccgcc tgcaccaaat
    25501 cttcgcacca gacaaagaag gaaaatcaga aaatgtttta ttgtcttacg atcgtttcga
    25561 agatgttcta acagacaaaa gcttttttgg agcaactgtc ggacctgtag ccggaaggat
    25621 ccgggatgct tcttggggtg agcatagatt ggagaaaaac tgcggatcac accatatcca
    25681 cggtggcacg aaaggctggt ctttccaatt ttgggaagta gaagttttca aaactccatc
    25741 atcaataggt gtggtctttt acttaaaaga tgaattttct acctatccag gaccaatcac
    25801 tgctacgatc acttaccgtt taacaaaaaa cgaattagag atgacaacct ctgctagtag
    25861 ccttgttgaa accatctgca atccgaccaa ccatgcctac ttcaaccttt caggaaatgg
    25921 aacaagagac atctatgaac atcagttaac ggtcttttta gatggtgtgc ttgaattaga
    25981 tcaagaaaaa ctgcctactg gaaattggac aaaaaaagaa aaactgccaa tcgactttag
    26041 aaaatcccca acactgcaag agatccttgc atgttatcca gatggactag atgatgtatt
    26101 tcttttgcat catcctaggc tttccaaaac ttccttacaa ttattcgaga agcattctgg
    26161 tcgccaaatg actatagcaa cttctaataa aagtatggta ttattttcta cgactggatt
    26221 cgaagctgat ttctctgtca atggaaaaca gatgcattca aactacggat tagcaatcga
    26281 accgcaagaa atccctgata tcgttcattt ccccaaattt ggatcgatca acctccatcc
    26341 tggtcaagaa cggatcagtc agacgattta tcggttctct gcacaataac aagagaaagg
    26401 tgtttttatc atggctatta ccgtaaaaga tgtggcgaaa aaagccggtg ttgccacctc
    26461 gaccgtctct cgtgtcatca acgatcatcc tagtatttcc gagacaacaa agaagaaagt
    26521 ccgaaaaatt atggatgaac taggttatgt tcctaacatg actgcccgca atctaggaaa
    26581 acgcatttcc agtgccatcg gcgtaatctt accccctctt gattcaaaag agcggatcgg
    26641 taacccattt tatcttgaga tcatggaagc aatcaacgaa gaagcgcgga attatgatat
    26701 gactacagca atcgctactg ctaaaaattt tgaattgctg ttagagaacg tccagcgtat
    26761 gcatttacaa aaacaagtag atggttttat tctagtgtat tcagatagtc aagatactgt
    26821 cattgattat ttataccaaa accacatccc tttcactttg atcggacagc cttatcatca
    26881 tgagaacgaa atcgtttatg tagataatga taaccagctt ctaggtaaac aagcaacaga
    26941 atttttgatc cagaatggac ataagaatat cctgtttgtg acaaacacga ctcatgaaag
    27001 tttgtatttc gaacgatatt tcggttatca aaaagcaatg atgatggctg gtcttcctgc
    27061 gttatccagt gtagtactag agcatccgga ggattatctg tcctttgaga aaacgttgaa
    27121 agaaaacgaa gccacagcat tggtggtcat tgacgatatc ttcgcccttc gcacgatgca
    27181 gttggcgcaa atgtatggct atgtcattcc agagacactc tccgtcatta gcttcaacaa
    27241 ctcgatcttc tcaacattaa cacaccctta ccttacaagt atcgatatcg atatctccga
    27301 attaggacgt gtcgctatgc aaaagctgcg cgaattgatc catgaagaat tgagcagtgg
    27361 cgtacgtttg gtcatccctc atcgcttgat caaaagagag acagtcgttc ctttagaaaa
    27421 cccatactaa atagctgact cactaaatac gctgaccatt tatcctatgg tcagcttttt
    27481 atgtaaaaga agatactact tctaacacat tgtttatctg taaaaaagag cgtgggacaa
    27541 aagtaaagaa cacttttgtc tcacgctcta aacacgtata aacggcggaa gcagaagcaa
    27601 ctccttcgga aataagctga aattcacaaa aatttaaaaa gcaattttct tgagtttctt
    27661 cttatttctt ggagttaaac gcttctgtcc cagcctcttt tttccttcct tcaatttttt
    27721 acacgtcgac gattcaataa aaaaccgcct aatattgtaa agacaggtgc aagaatcgag
    27781 aaaaaggcga atggcaagta atgcaacgcg ctaactttca tcgttcccat aatgaatgtg
    27841 ccgctgactc cccacggtac gagagaattg acggtcgcac ctgaatccgc tagtgttcgt
    27901 gtcaaatagg tttttgataa gccaacgtca tcaaaagaac gcttgaatgt ttctcctgga
    27961 agaatgatgg acagatactg ttctccaacg atcagattga ttccgataca gctcaaggca
    28021 gtaaacccga ctaaacgaga aggtcggtcc atcttttctt ttaattcttg aacgatggta
    28081 tcaacgatca gatatttgat cagtaaccca cctaatgcca atgcaagtaa aatcaaacta
    28141 gcagaaccta acatactcat gatcccgcct cgagaaagca gttcatctag ctttggatca
    28201 ggtgtatttg cgacataacc attcatcaag atgtctgctg cttttccgac cgtcaaactt
    28261 ttctcatgga taaaagaaag aacgagagca gctgtcgatc ccgctagtaa tgtagggata
    28321 gccggaattt ttttgattgc ccccccaaat aaaataacta gtggaatcaa tgtccaagga
    28381 gagatccaaa agctcgcttt catgttttgg atcatttcat tgatactagc tagatcggct
    28441 gaatgactgt tatgtcctaa tatcgtataa aaaatcaacg aaaggacgaa tgctgggaaa
    28501 tccgtataac gcataccagc gatatgttcg aatagattaa ctcctccaat acccgctgcc
    28561 aagttggtcg tatctgataa aggcgagcag ttattcccta aaaaggcacc ggaaacgact
    28621 gcgcctgtag tcattgcatc attgaatcca agtaaatgac cgatccccaa aaaagcaatt
    28681 ccaatcgtcg aaatcgttgt aaatgaactg ccgacagtca ctccgacaat tgcacaaaca
    28741 agaaatgctg tcggtaaaaa gaaacgcaca gaaacaagtt taaatccata aaccataatc
    28801 gtagggatgg ttccagataa aatccatgaa gcaactaaga caccaatcaa aagaaaaatc
    28861 aggattggaa tgatccctgg agtgattcct ttgatgatcc cgtcatggat ctcatcccat
    28921 gtgaactttt taaatctgcc ataaaacaac aaacacatga aagctgcaag gatcgggata
    28981 tgtggcgaga gctggaatcc gatgatcagt actcctagga gtgccaacaa cacgatcaat
    29041 acagcgatac tttcttttaa agaaatattt ttattcatcg tcgatctcct atttcaaatg
    29101 aaaaggccct gccatgaaaa aactattcac ttggcaggac ctcctactaa atcagcgaca
    29161 aaacgtcggc tgaaagactt atttttttat ttttcttcta aacttttttc tacctcaatc
    29221 ccccaagaat ctttcttgaa gtgattgatg atcaatggaa taacgaacat gaccactgtg
    29281 atagctgcta ctaataatgc atcactcaat gtcgctgtac ctaatgtagc aaatacgaca
    29341 acgatgatag agaagatcag catgcagctg actacccaag ctaatccgtt tccgttttta
    29401 ccaattcggt aaggacgttc gaattctgga tgtttcttac gcattttaag caatgaaaca
    29461 gcaatcaaaa tgtacacgat acaataaata acagttgtag catttgtaag tgtcaagaat
    29521 acagcactta cgtcatccat tacaccataa agcaaagcaa ataatgaaat aacgattgat
    29581 tgtgttagaa cgacatttct agatacgccg tatttgtttt ctttatggaa tccgaatttt
    29641 ggtggaagga atccttcacg agctacttgg atgattgttt ttgatggtcc agtaacccaa
    29701 gctgataatt gtaaaagcac accaataaat accatgaagc tgaagatatt tccgatcact
    29761 tctggtaagc ctaggatttg acagtcaatc aagataggct gcgtgatatt tgctaattcc
    29821 atttttccat ctggtacgac atttgctaca agcatcgcat tgatgacatt caataaaact
    29881 aatccgatca aagcgataaa tacgcctttt gtataatttt ttgttgcgtc tttcaaacga
    29941 ggcatgtaaa cagaactcat ttcgatccca acaaaaataa atgtgatacc agctaagtat
    30001 ttcaatgaat caatatgttc caagtcaggg aaagcttttg accatgaaaa gtcacctaga
    30061 tagccgtttg agactagtcc gactttgaag gctgccaaaa cacctaaaac gaacatgatg
    30121 actactggga tatatacccc tagccataca ccgatgttcc cgccgacttt tgccatatcg
    30181 aatttcaagt tcaagatcgt gatgatccag tagataacta aaatacaacc taaaacgaac
    30241 cagtgattat tgccaagttc tacattacca aatgtgtttc ctaacaatgg tcctaaagta
    30301 gaagcgacca ttaccatacc tgggaacatt tgcacccata atagccatgc aacgacaaat
    30361 ccccatttgc tgcccagtgc tgtttttacc cataactgag gaccgccttc ttgaggaagc
    30421 atcgtggaaa gctcaccgga aaccattgat atcggaccgg caaaaaacaa aactgcaaaa
    30481 attgaataaa aaatcaatgt ccaaccgact gcggctaacg taggaatact ccgaaccgtt
    30541 gcacacagtg ccattgtcag accgataaag gtcccaagac ccattttact tacttcagat
    30601 ttactcaccg ttgacactct ccttgatttt ttggataaaa taacggtgtc tttgaagtag
    30661 tttcagcaat aaacgaaaca attcagcgaa tatttgaggg gattctttga gtttattgct
    30721 tgaagggttt cttcaaagac aacctcaaaa caaagggatc gaagagctta ggtctcccct
    30781 tgctcttcag atccaaacat gcgtcagaaa cagttagtat tcaagctgtt ttctaacatg
    30841 attaccttgc ttattttgct tcgcttgcca ataattggtc tgcgtagatt ttttccaatt
    30901 tttcttgaag agctgctttg attttttcag catattcttc aaagtgttct tctttgttca
    30961 tgtatggtgt cattacgctg gcacgcaata cagttacttt tccggcacgg ttccattctt
    31021 catctgagaa tcctagttgg ttcacaaatt gtaatgggct gttgccatag tctgggatag
    31081 caaagtcagt atgtgaagtt aagaactcat taccgtaaat gcttcctttc acgtatgaag
    31141 aataatcata tacgtcatgg ttcaatttat tcattgcaac taagtcatcg ttgccttttt
    31201 ctttgaatac atagtctacc atattgaaat ctggataagt taatggatga acttcgattt
    31261 ctttgtctcc tactttaaat gacaagtcat tcaagaagtt gtagaaacga tgtgcacctt
    31321 cgattgatgc acccattaat ttaccatatc ctgtgacatt caaaggtaag acatggtgtg
    31381 ctgcccaaac gctggctgct gttgcccctg cttttgaacc ttccaagata taagccccaa
    31441 gtaatgctgg gatatctgcg cctttttcaa atacatatgt cgcaaagtaa gagataacgt
    31501 ctctcatgcg gatatctttg atgacgatac caccggcaga atatggaaca tagcccattt
    31561 tatgtgggtc aatcgttaca gattctgctt cttcgattgc tttatatgca ctgtgtactt
    31621 cttctaagat ataatctttg ttttctgtaa agacattgta tttgtaatga acatctttta
    31681 aatcttcaaa tggaatgaag ttgttgtctt cgtctaagaa aatcgcacgg ccgtatccgc
    31741 cataagcagc atctacgtgt aagtagaagt aaataccgtc tttttctaat acgcgacgca
    31801 atgcaacgat tttatcgata ccgtcgattg ctccttcttc tgttgatcct acaacaccga
    31861 ctacacctag gattggtgtt ttttcagctg ctagaccacg aacgattttt tcaagttcgt
    31921 tgatgtccat gcgatagttg tggtcaactg gcactggaat cacttggtct aaaccgatac
    31981 cgatgatgtc ggcagctttt agccatgaat agtgtttagt ttgtggaacc aaccattttc
    32041 ctaatttttc taaatgtttt ccgctacgtg ctgagtgagc tttgatttcg tcgatttttt
    32101 ctgggacgct gtctaacaga ttcatgattt cttctgttga caagttcatt aattcccaat
    32161 cgctttttcc agcaactagt tcaggtgtca cttctttcat tgcaagcggc aatgatttga
    32221 tgttgcgggc ataccaaagt ccttctaagt ttgctaaaga accatcagca acgatgtggc
    32281 cccagccatc tttatagctc attaattttg caaattccat tcctacttct tcttccattt
    32341 ggcttgttgc aggagaagat tcataggcaa cgttattacc attccatagc atagcaaaat
    32401 tgtacgctag taaagatggc atcaatgttt cagagttcat atgaccccag taacgtccgg
    32461 cattatgcca aggaacggaa tgggtacgca tccgcgctga aatttctgat agaacatcct
    32521 ttgttttatt cacagtgtgt tcaaaactag cactgctttt ttcttctggc gtaataatag
    32581 gcatgtcttg aggcatgtag ttttgacgcc agcctaaatg ttcatccacc aattcattca
    32641 acaaagcttt atagatttgg ccgttctcag ctttgtcccc aatgaatagg gcatttaggt
    32701 ttagatcttt cgacaatgat tcactcatag tgaaaccctc ctaaaaaaat atttaaattt
    32761 tgtcgagccc tacattacac ctgttcttct attctgtaaa ctaagttttg gcctgatttt
    32821 taatcatatt ttaatatgta tcaattatcc gaattgttca cacttttgaa ataattaaaa
    32881 aaaacgaggg aatttacaaa tgtaaactcc ctcgcaaaga ttgtattaac gacaaccagc
    32941 gttctatttt aggatgggtt tcaaaattgg aatgttgtaa tagtatttcc ctttttcaag
    33001 gtaaaaactt actaaattct aatcaattac tttggccaaa aagtaatttt tcttaccttt
    33061 tcgaataacc acaaacttac catcgaattc atcggatgga tttatgacaa aatttaaatc
    33121 ggtaacgcgg tcaccgttaa tacttatagc tccgtttgaa acatcttcac gtgcttgacg
    33181 tttagatggt tcaatttttg tggaaacaag cagttccacg atattttcag gtgtgcttga
    33241 aatttcaacg ttcggcattt taccgaatcc ttgcgcgatt tcttccgcat ttagttcttt
    33301 aatatttcca gagaacaatg cttgcgttat tttttgcgct tctttcaagt cttcttcact
    33361 atgcacaaat cttgtaactt cttctgccaa acggcgttgt gcttcacgtt tctcaggttc
    33421 tgtctctact tttttcgcta agtcttcgat ttcttcttga gacaagaaag taaagaattt
    33481 caagtatttc actacatcgc gatcatcttg attcaaccag aattggtaga actcaaatgg
    33541 tgaagttttc ttcggatcaa gccaaacggc accgcctgca gtttttccga atttcgttcc
    33601 gtcagccttc agcatcaatg ggatcgttaa accgaacgct tctgcttctg gtccttctaa
    33661 ttttcggatt aggtctaatc cagctgtgat atttccccat tggtctgctc caccgatttg
    33721 caattgcaca tcgtatgttt tatgcagatg caaaaagtcg atagattgca ggatttggta
    33781 agtaaactct gtaaatgaga ttcctacttc caaacggctg gcaacgatat cttttgctaa
    33841 catcgtgttg atgttgaagt ttttaccgta gtctctcaaa aattcaagta aagagatttt
    33901 tgacaaccag tcgtagttgt tcacaaatgt gatatttgca tctttaccaa acaattttct
    33961 catttgattg gaaagtgcgt ccacgttatg ttgcacttgt tccatcgttt gcaacacacg
    34021 ttctgttttg cggccgcttg ggtcgccgat cgttcctgtt ccgccgccaa tcagaatata
    34081 tggatgatgg cctgccaatt ggaatctttt catcatcata aatggaatca aatgtccgat
    34141 atgcatcgaa tcaccagttg gatcaacgcc gcaataaagt gaaatgctct tttcttcaac
    34201 gagctcgcga agtctttctt cgtttgtttg ctgattaatg gcgtcgcgcc aagttaattc
    34261 atcaataata ttcattttac tgacctccgt gtagtttttt tatgaaagca ggaacctgct
    34321 agcatatgta attaattatt tcttcaggat aggataggaa ataaaaaaag tcccttggca
    34381 tataagccaa gggacgaaat ctcgcggtac cacccaattt gtatgtctag atatccaaca
    34441 tacctctcga acatttaacg ctgtcgcgtg gcaatcactg ccaaagactc ctgaaatgta
    34501 attcgcttta aagcatgtac tggtttgcag cgaccaccag ctttctgaaa cattggcttt
    34561 aacaactact tcgtttcgat cttcgtcttg ttattattaa tatgtgtcta tcatagagcg
    34621 caactttttc tttgtcaaca gaaaaaggat atttttttta aattcctcat tttttctttt
    34681 ttgaatccgc tctaattccg aacattttat tttcaattat tgttaaaaga aataactttt
    34741 tctgtatttt ttctgcaatt tatcgagaag tcactttact ttgcctctta ttctttcttc
    34801 gttacaataa ggaatgggac aagtaaaaaa atgaaaaagc aagggacgag gtatatggaa
    34861 aaaaagcaaa aaattatttt aggagccact tctgctttaa ctatcgttat tttaattcta
    34921 agtttgtttt ttgtcatccg atcatctaca aaaaaagaac caatcattcc tgaaaaaaaa
    34981 ataacagaaa aagcaaaaga accgacagat gataagcaac aactagttac tcaagcaaaa
    35041 aatctggctc aaagctacga ttatgaccaa gcgattgcgt tattggaaaa agatagtaag
    35101 aacggatcta ctgaaacaca acagcttctt caaacactaa aaaaggagaa agaacaactg
    35161 atcgactggt ctgatcctac tcagatctct catgtctttt tccacagctt gatcgtggac
    35221 ccgcaaaaag ccttccactc tcaacaggca caaggataca aagattatat ggtgactgtt
    35281 gaagaattca accgttcgat cgaacaactt tatcaaaatg attttgtttt agttaatttg
    35341 aatgatttga tccaaaaaga cgaacaagga aatctttcct ttaacgggct tgcgctacct
    35401 gaaggaaaaa aaccattggt tctttcacaa gatgatgtca gctattatga atatatggat
    35461 aattccggtt ttcctagccg attggtcgta tcaaataaca atgaaatcaa aaatgtctat
    35521 aaagagaaag ataaagaaga aatcggtgac tttgacatgg ttcctctgat cgatgccttt
    35581 gtcaagaaac atcctgattt ttcttatcga ggcgcaaaag gtaccttggc cctgacaggt
    35641 tacaacggag ttttaggtta tcgaacatca aaatctcaat atggagacaa tgaaaaaaca
    35701 aaaaaagaga ttaaagaagc taaaaaagtg gcggaacagt tgaagaaaga cggctggagc
    35761 tttgcttccc atacatgggg tcacttgaat atgactcaag cttcactaga agatgtaaaa
    35821 aaagacaatg aattgtggca gcaagaagtt gcacctatcc taggaaaaac aaatattctg
    35881 atttatccat ttggtgcaga catcagcgac tggcagcctt attcagcaga aaatcaaaaa
    35941 tttgcttact tgaagagtca aggtttcgat attttctgta atgtcgatgc ttctacacca
    36001 gcttggggac aattaggtac ggatttctat cgaaatgctc ggatcaatat cgatggcatt
    36061 cgttttcaat ctgatttaga cggaaaaaat cctattcttg atccattcat caatgtccat
    36121 gatgtctatg acaaagaagc acgcgaatct gcttgatttt acgtcattta tgaaccaaaa
    36181 aagccgaact gacattttat cgtcagtttc ggctttttta tgctttattt cttcttcggt
    36241 gcgattccct tgatcaggac ttctgtccat tctgctgtat atggaagcag ttttgcgtat
    36301 atctccggct tagaaattcc ttctaccatt gcttgaaaat agaggatcaa gaactcatcc
    36361 gaatatttca aatccacttt tccttctttc cgccctcgat ggaaaagatc aagcatgata
    36421 ctgaaacttt cttgcgtata cgcatccatc gcgtcagaaa attctttttt ttcttttttt
    36481 gtatagtatg tcatcaaatc gatataaaat tgtttgttca ctcgattgag ataacggatt
    36541 ttgttttcac tcatggcaat cagtgtttct tcaaatggaa gattttccgc aatcaacgct
    36601 ttcgcttctt gccctaaatc attcataaat gcaaaaaata cttcataaat cagcccttcc
    36661 ttgctgccaa aatatttaaa aatcgtcgct ttacttacct gagcgttctc agctacctta
    36721 tccattgtca ctccagcggt tcctatgtct gtgttcatca agtcaaaagc tgcttgtaaa
    36781 atctctttct ttttctcttg tgctctcttt tcaaaaccgt tcatttgtag agcctcccgt
    36841 aattttaaac ttttataata tatatagttc aaat
//
LOCUS       DAZKLA010000015        36791 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000015 DAZKLA010000000
VERSION     DAZKLA010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.015"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(533..1036)
                     /locus_tag="ACG85N_000612"
     CDS             complement(533..1036)
                     /locus_tag="ACG85N_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0223408.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1395..2420
                     /locus_tag="ACG85N_000613"
     CDS             1395..2420
                     /locus_tag="ACG85N_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0223409.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2743..3621
                     /locus_tag="ACG85N_000614"
     CDS             2743..3621
                     /locus_tag="ACG85N_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFQ0223410.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4097..4213
                     /locus_tag="ACG85N_000615"
     CDS             4097..4213
                     /locus_tag="ACG85N_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0223411.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5141..5479
                     /locus_tag="ACG85N_000616"
     CDS             5141..5479
                     /locus_tag="ACG85N_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223412.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5573..5803
                     /locus_tag="ACG85N_000617"
     CDS             5573..5803
                     /locus_tag="ACG85N_000617"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFQ0223413.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6203..6574
                     /locus_tag="ACG85N_000618"
     CDS             6203..6574
                     /locus_tag="ACG85N_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFQ0223414.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6848..7540)
                     /locus_tag="ACG85N_000619"
     CDS             complement(6848..7540)
                     /locus_tag="ACG85N_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0223415.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7543..8328)
                     /locus_tag="ACG85N_000620"
     CDS             complement(7543..8328)
                     /locus_tag="ACG85N_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0223416.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8565..9965
                     /gene="lacG"
                     /locus_tag="ACG85N_000621"
     CDS             8565..9965
                     /gene="lacG"
                     /locus_tag="ACG85N_000621"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFQ0223417.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9990..10307
                     /locus_tag="ACG85N_000622"
     CDS             9990..10307
                     /locus_tag="ACG85N_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0223418.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10320..11993
                     /locus_tag="ACG85N_000623"
     CDS             10320..11993
                     /locus_tag="ACG85N_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFQ0223419.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            12007..13638
                     /locus_tag="ACG85N_000624"
     CDS             12007..13638
                     /locus_tag="ACG85N_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFQ0223420.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(14129..15694)
                     /gene="guaA"
                     /locus_tag="ACG85N_000625"
     CDS             complement(14129..15694)
                     /gene="guaA"
                     /locus_tag="ACG85N_000625"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFQ0223421.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15753..16676)
                     /gene="coaA"
                     /locus_tag="ACG85N_000626"
     CDS             complement(15753..16676)
                     /gene="coaA"
                     /locus_tag="ACG85N_000626"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFQ0223422.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            16872..17477
                     /locus_tag="ACG85N_000627"
     CDS             16872..17477
                     /locus_tag="ACG85N_000627"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0223423.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            17785..18801
                     /locus_tag="ACG85N_000628"
     CDS             17785..18801
                     /locus_tag="ACG85N_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0223424.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            18942..20243
                     /locus_tag="ACG85N_000629"
     CDS             18942..20243
                     /locus_tag="ACG85N_000629"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFQ0223425.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            20289..20951
                     /gene="deoC"
                     /locus_tag="ACG85N_000630"
     CDS             20289..20951
                     /gene="deoC"
                     /locus_tag="ACG85N_000630"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFQ0223426.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            20972..21370
                     /locus_tag="ACG85N_000631"
     CDS             20972..21370
                     /locus_tag="ACG85N_000631"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFQ0223427.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            21436..22518
                     /locus_tag="ACG85N_000632"
     CDS             21436..22518
                     /locus_tag="ACG85N_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0223428.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            22730..24292
                     /locus_tag="ACG85N_000633"
     CDS             22730..24292
                     /locus_tag="ACG85N_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0223429.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            24285..25418
                     /locus_tag="ACG85N_000634"
     CDS             24285..25418
                     /locus_tag="ACG85N_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0223430.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            25418..26374
                     /locus_tag="ACG85N_000635"
     CDS             25418..26374
                     /locus_tag="ACG85N_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0223431.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            26473..27639
                     /gene="deoB"
                     /locus_tag="ACG85N_000636"
     CDS             26473..27639
                     /gene="deoB"
                     /locus_tag="ACG85N_000636"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFQ0223432.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            27663..28481
                     /locus_tag="ACG85N_000637"
     CDS             27663..28481
                     /locus_tag="ACG85N_000637"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0223433.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            28498..29205
                     /gene="deoD"
                     /locus_tag="ACG85N_000638"
     CDS             28498..29205
                     /gene="deoD"
                     /locus_tag="ACG85N_000638"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0223434.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(29503..30189)
                     /gene="gpmA"
                     /locus_tag="ACG85N_000639"
     CDS             complement(29503..30189)
                     /gene="gpmA"
                     /locus_tag="ACG85N_000639"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0223435.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(30361..30942)
                     /locus_tag="ACG85N_000640"
     CDS             complement(30361..30942)
                     /locus_tag="ACG85N_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0223436.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(31075..31758)
                     /gene="rpiA"
                     /locus_tag="ACG85N_000641"
     CDS             complement(31075..31758)
                     /gene="rpiA"
                     /locus_tag="ACG85N_000641"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFQ0223437.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            32077..32490
                     /gene="rpsL"
                     /locus_tag="ACG85N_000642"
     CDS             32077..32490
                     /gene="rpsL"
                     /locus_tag="ACG85N_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFQ0223438.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            32574..33044
                     /gene="rpsG"
                     /locus_tag="ACG85N_000643"
     CDS             32574..33044
                     /gene="rpsG"
                     /locus_tag="ACG85N_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFQ0223439.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            33138..35225
                     /gene="fusA"
                     /locus_tag="ACG85N_000644"
     CDS             33138..35225
                     /gene="fusA"
                     /locus_tag="ACG85N_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFQ0223440.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            35397..36584
                     /gene="tuf"
                     /locus_tag="ACG85N_000645"
     CDS             35397..36584
                     /gene="tuf"
                     /locus_tag="ACG85N_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0223441.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
BASE COUNT        11499 a         6553 c         7824 g        10915 t
ORIGIN      
        1 aataaaaaaa taacatatct cattttacac ttttgttatt ttatacttaa aaaataacga
       61 cacagaaaat accgtgtctc agatttctct actacttcta aaaactactg tttttttcct
      121 ttgaaatgcc ttcaaaaagt aggatcaggc agaatatact ggcagaatca aggagaaggg
      181 agcctaaata aggagaaaaa ccatagaaaa tataaagtaa ttacttataa aacaagcttt
      241 ttatagaaaa ataatatacc tgagtagggt ataaattaag acggaattcc tttctagatt
      301 gagatattga ctaggaagaa tgttaagatt ttaagccttt aagctaaaaa aaaacccttt
      361 ttgttaaata aggggaatat agcctgtcag tttcaaactg caagtcgtcc tgctgcaata
      421 atatagtaga ttagagatga aaatagattt ttttaaacca atgactgaat aaaaagaccg
      481 tcatagatac acattggaat gtgtctatta cggtcttttt ttacgaacga ttttattgaa
      541 gacggacagt tcgggtcaaa aataaaaaag ctacgatcag aaataagaat aaaatgtatc
      601 ccaaaataat ggctagacgg tctaacttat ttgtcgatag atgtttgttt agaagttgat
      661 aggcccataa acctatcagt cctcctgttg tattagttat caaatcagtg atgtccgcta
      721 cgccaatacc gaataggaat tgaatcaatt ctgccaagaa gcttacaagg aaaataatcc
      781 ctaatttttt agaaaaagaa agtctcttga aattgacatt caaaagcacc ccaaaaggaa
      841 aaaagacaat caagttataa gtggtctcac gtaataccgt cgtattttca aaagaaaaag
      901 tagaaaaagg gacgagattg acattgctgt aagaataagt gagaaattgt ggaagattaa
      961 atgatagctt gaataaaatc aaccataata gaaagaataa ataaatccct aataaaaatt
     1021 ttgatacata ccgcatagac atctcctcca tttaaaaagt ataccatcga aaaccgctga
     1081 aaaatcataa aaaagtatga agtttaagga ccaacgtagt tatcgtacga aaattagaag
     1141 cttttttttg ttgggaatcc ggtttcttta acaaagctaa gatagcgagt tgcttggaaa
     1201 agttgctatc ttttttctgt caaaacgact cggaatgttc gttttttgtt ctaatttctc
     1261 caagttgttt actttttgat actaagttta tgaaaaatca agaaatagtt gttatactag
     1321 gtaatgctga caaacgggat tggcatttca tcttggttca gctttttttc caattactag
     1381 gaaggaggga tagattgttc aagacttatt ttcgtttcaa taaaatacaa gataatcctt
     1441 ttcgtatcat gaatctgatg ctacttatct ttattgtatt atggttaggt tacataaatc
     1501 atgatttttt gatttcaagt ttttcatcat tagggatctt tacttttttc tattatcaaa
     1561 acataccgat gaaatatttg ctgaaacgta tgtttctgat cggttgcgga ctgttggttg
     1621 ctttcgtttt ggggatctta tcgacttatg ttttatggct tgaaccattt gccgtagctt
     1681 tggttgcttt ttccagcaga ttcgtgttgc gattattcca tatttctaaa ccaggtggct
     1741 tgttttttgc gatgctttct gccatgggaa cgagcatgca gctgccaatc gctcaattac
     1801 cgatcgtctc actttatttt tttatgggtg tcgtttttgc cctgattgcg gcagtgatta
     1861 caaaattact ggattcacgg cctgagcaga caattgaaaa agcaacttta aaagaaagat
     1921 ttcacgaaga gcctttagtg atcattgatt ctgttttcta cagcgctgcc ttatttcttt
     1981 ccgtttatgt cagtcacggc ttgaatctgc ataatcctta ttggttgacc ttgtcatgtg
     2041 catcgatttt gctagcagaa aacttggatg cgatgaagca tcgacaagtc caatatctga
     2101 ttggatcgat gggtgggtta tgtgtgtcgg cattcttgtc atttgtgccg tttacgcagt
     2161 tgcagaccat ttttctgatc acttttctat atggaatcgc acaatttcta gttgctagaa
     2221 attatgcagt tgctaatatt tttcttaatc cgatggcgtt gatgctatct acacttattc
     2281 gtggagcata cttgatttct ttgatcgaat atcgtttttt agggatcgtc ataggaagtt
     2341 tcatcggttt aggtgttgcg tgggtaatga cggttggttt gcaacattat ttaacggttg
     2401 ttcgtcaaaa attagaatag aaggacaaat atatgtgcca aaagaggtta tgacaagatt
     2461 tttgtcacaa cctctaattc aaggtgttca aaagccagat tctcagcatt aagtcaaatt
     2521 cacacaaaaa tgtggaaagg aactttcaaa aaaattttat atttattcta tcaaaaaatt
     2581 ttgtttatga tggtaactga ataaactttc ctgagatggt caatgctttc cttgaaagtc
     2641 ttacagatat tctttcttca tagaaagttt aagttttata gttagtgtac aataaaagta
     2701 tgtttatacc atatggtgtc cggataatca gtcatttaaa caatgaggtt caaagatatg
     2761 aaaaggggaa atttgatgaa gaaaataagc aatgaaatcc aaattctggc agaaacttta
     2821 actcttccca aagtcgttat agagcaaatg attgtacaag caaaaaacat ccttgatgag
     2881 gtgtttgagc aatatagtca tgaactaatg gatcctaata aagcaaaggc gactgcaaag
     2941 aaaatagaca aagtctatga agaggaacct tttgcagcat tagctattta tctatatgca
     3001 gcttctcaaa gttggaaaaa aatttatcaa ccattgaaga ttccaagaac tatctatttg
     3061 gcaacaatga atgcttttac tagatttatt catgaacatt tccaagtaac aggaagctat
     3121 cgctttgacc gtggtttttg gatttggcat tacactagtg gcttgatctt tcggattggt
     3181 gaattggagt acgaaatgac atattttccg aaagggtaca cggatctaag attagaagga
     3241 aagcagtgcc tttctcttca tattccttcc gatgcagatt tatctcagaa taagataaga
     3301 gatagttacg aaagtgccat tcgttttttt gaactttatt ttcctgatta ccattaccaa
     3361 gtgatgtaca cagatacttg gttactatca cccaatctta ctaaatggtt gaaaaaagag
     3421 tcaaaaatct gtctttttgc agccgattat cgattattat cagttgatga acaggacgat
     3481 agtggagtcc cttggatctt tggacgagta gatgcccaaa tacatgatta tccagaaagt
     3541 acgagtttac agcgacaagc aaaagagcaa ttgcttgcag gcgagcacat tggctctggc
     3601 ttaggtattc ttacttcata agtttagtac ttcgaaatag tttaaaagag cgtgagacaa
     3661 aagtaaagaa cacttttgtc tcacgctcta aacacgtata aacggcggaa gcagaagcaa
     3721 ctccttcgga aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaatttctt
     3781 cttatttctt ggagttaaac gcttctgtcc cagcctcttt tttagtgttc tatcgaacac
     3841 acagtacgtt caataggtaa actagctata acaatagata ttaaagttaa cggacaagag
     3901 ggcctttttt aggtagctaa aacgtaataa tgaaaattta tttccagaga taggagtcaa
     3961 tcagctagtc tgatcgactg ttttaacaag ttgactttca ttttaacttt tgataccatt
     4021 taaaacacaa atcgtaattg tttcgatttg tgttttttag catgactaca atcaaagaag
     4081 aaagtgggtc gtccatatgg aaaaaacaga tatctcaagt gcataccgcc gcttgaaaag
     4141 cccaaatatc aaaacacgaa aacgtgcgct gaagatcatc aaagatgtga aacggaatag
     4201 tgggaaaagg taagaagaag gaaacttaat taaaaaaata gccgaaaaac gtttttcggc
     4261 tattttcaat aatcggtttt tgtgtaaggt ttccgaaaga actgctgttg attttccatt
     4321 tttaacatat gataaattgg tagaatcttt cttttcaaat tgataccatt ccctaattcc
     4381 agttatatag ttaactacag aaccacaaag cacgtacgtt tataaaagta ttatctgtag
     4441 ttatttttca attccctttc aacctcttat tttgaccagt agtattgatc actttacctt
     4501 ttaacacctt tgttcgtttt tgaaacattt ttagtcaaga atggaaacgt aactacataa
     4561 tcaaaggttt tgcgaaatat ttcatgaaat atttcatgta gaagcgagca atacggttgc
     4621 aaataaccat tatcgtttaa taagcagtta cgaaagctcc caatatctct aacgttcagc
     4681 tcactccatt tcttttcttc aaaaaaatca gtgattcgta tctctttcta tttgtcgaaa
     4741 aagttaactt taaaaagtaa cgccgaaaaa atatgcaaaa aaataaaaaa gcagttacta
     4801 ttgctagaat aacatcaagg aataataagt tttggttttc agataatagt cgataagttg
     4861 tgtattggaa ttttagatac agtaataaag tatttttcat gaaattttgt gagaatcact
     4921 attacaagta tctttaataa aattaatggt taaggaggaa cttaaagtag aatgtaaatg
     4981 aatagttgta gtcagtcgat tgtgatacta gactcaaagt tgcaggaata agtttttcaa
     5041 tttgtgattg gaagataaaa aatgaaaaag aagggctttt ttttatactt tgttgattgt
     5101 agtgtttatt ttccctctgt ttagccaagt aacattttct atgactcaac cagtttcaag
     5161 tgaggaacat gataaccttt ttcgtgctac agaagagttt aagaaatatc aggaaaatat
     5221 tgttccggag actccattag ctagtgagcc aattactgat attgatggaa accaaatagg
     5281 atggtatata cagtacgaag ttatggtggt aaataatgac tcaatagtaa aaaatacagt
     5341 aggcttagag ctaacaccga ttgaaaaggt gagttcattg ttaacgtata tttatactga
     5401 tgaaagtgac gagactgaga tcattttaac ggattactca aaaattgcta ctgataaaaa
     5461 gtatatattg tggatgtaga cactagtgaa atactttatg atatagaaat tgatgaaaat
     5521 ttactagatg tagcacaaga aatcgatcat caaaagcagg aagctattcg atatgctggt
     5581 agaaaaggga aaagaaaata tttattacgt gaatgtagca aaagttagag aagatgaaaa
     5641 tgagtggaaa gaattcaaga gtcgctactc aattaactcg actccgacat ttacagttta
     5701 cagagaagga tcgatagaaa aaactgtttt ttggactaaa gaaagtggaa tgtctttagc
     5761 tgaagtagaa gagtttttgg attatgttag tatgcaacag taagggagtt cttattatga
     5821 aaaattttga cacatattat gttattgttt tttcgatatc tttgctgtta atattactaa
     5881 ctttaatgca gtataaagag tatccagagt attttatttt aacttttgta tatttagtaa
     5941 gtagctacac acttctatat aaattcagaa gttcttctag agtttctaaa gtttgtttct
     6001 taattagctt attgattcta taatattttt gatttatata ggaaattaaa aatattatag
     6061 aatcaacact cagagaacgg gttggttttt aatagattta ataagtactg ataatgaaat
     6121 ttctcctggt tgggaaaacg aagttggtta ccctgtagaa gaattttttc aagattactt
     6181 tgcggagtat gccgatagga taatgagaga ttacgtaaca gataaaactc aagaagtcat
     6241 caatcaatcc cagtcattaa atgaattatc tgataaggcg aaagaagaag cagttgccat
     6301 tatttcttca gggattagtg actatattga tatgagtggt gaaatcaatt atggcgataa
     6361 agacaaaatt gtacgcatga taaaagcttt tatcaaaact tttgatgtga aggcagatct
     6421 taagtttaat gatacttacc tagtaggaaa attaattaat atccttggga cagagaaaga
     6481 tacggaaacg tttatttcct ctttgtctat gttgtttaca gataagcatt tttcaactca
     6541 gcttggtgga gaggaactag tggaaatctt ttaaccatta cgcccagcat taataaaagt
     6601 atatcaaaaa agtactcctg atgattttta agattggttt gagaaataat gtgaaaacac
     6661 tatttttaat gttaattata gatagagatg aacgcgtcat atggttaaaa gaatgaaaaa
     6721 gtcaaagata cactgctata tttcactaag gatatagcag tgtatctttc atgtcgtttc
     6781 actggcagtt ttttataaaa ggaggtgtgc atttcagttg cttttctttg gtagttagtt
     6841 gaatcgttta agaattaacg gatgtggcag acaggtgctc tgtccaaaaa ccagtaaaat
     6901 ctttgatggt tccatcttta tttagctgtg cctgttcaaa tactgttttg tttttaccga
     6961 atggatcaat tgcaataatt gttccgatcc ccgcacgttt ggcagcaagt aatcctgagt
     7021 aagcatcttc gataacaaga caatcagccg gagctaagct cagtttgtct gccgctttaa
     7081 gaaaaatatc cggttgcggt ttcccaggga aagagccgtc gtcatagaca atcttgtccc
     7141 agtcaaacca gcgggctaaa tcgaaaatat caaaataaaa agcaacattc tctttgacag
     7201 tagccgtggc aatcgttaat ggaatctgtt ggtcagtcag ctggtcaaga gtcgcttcaa
     7261 gcccttttgt caactgaagc tcgttttcat tttgcaagca aagcatacga taatagcttt
     7321 ctttttcttc tgacaatacg gcgatctcag aggcggtcaa atgctctgaa atgaaatgtt
     7381 tcaaaatctc cgtattcgtc cgaccatgaa tattttttaa aatatcctcg tctgataaac
     7441 catcttttgt atgatcatgt atcatatgca gccaagcagc ttcatgcaga tgagagtcca
     7501 aaaacattgt tccgttgaaa tcaaatataa tccctttcat agttacgctc ctatagtggt
     7561 attttcggtt gaatcagcaa tttgcagatc gacatattgt gcatagtgct cttgaacctc
     7621 ccgcgagatc tggcgatccg taatgagcag atcgaaatca tgcaaattat aaaacacata
     7681 aaaatcatct cgattgaatt ttttattatc aacgagtaga tatttcttac gggaattatt
     7741 tagagcgatc tgctgtgctt ctccttcttc ggtactgtaa gtagagatcg cttcgttgtg
     7801 aacgccattt gaactgataa atgcttttgt aaactttagt cttcttaaat tgtcgttggt
     7861 gatcggtccg acaaatgcgc cggtattttt ccggaaatca ccgccaatca gtagaatttc
     7921 tggtccatct gcttgagcaa gaatatcaaa aactggataa ttatttgtaa tgatccgaat
     7981 tttcttgttc agtaaatgcg tcgccaatag ttcgattgtt gtacctggtc ctaagaaaat
     8041 cgtttctccc tcgtgaatca aggaggctgc tgtggcagca atttcttttt tttcttgaat
     8101 ctgcagagtg gctttttcag tgtgagaaag ttcttgatct aaagtgtagg aaagactttg
     8161 agcccctcca tggacacgca gcagcttgcc cgatttttct aattcatcta aatctctgcg
     8221 aacggtcata tcagaaacat ctaattgatt gatgatttca ttaacggtaa ggattccttt
     8281 tttattgacc atttctacga tagtaagcaa tctttctttt ttcaacattg tcttcacctc
     8341 taatttaagg ataatctatc atataaacgg acaacagtca atctaattcg aacaaaagta
     8401 aacatttagg gtggttttta tgtttgttac aatagatttt attgctgttt tctggttttt
     8461 atgattattt gtgtttgttt ttgttgacaa atgttttgga tgcgtttaca atgcaatcag
     8521 ttagaaagag cgaaaactga ttatagattt gaggagagat gtacatgctt aaattaccag
     8581 aagattttat ttttggcgga gccacagcag cctatcaggt tgaaggagcc actaaggaag
     8641 gtggcaaagg cgctgttgct tgggatgact ttttagagga acaagggcga ttcagtcccg
     8701 atccggcaag tgatttttat catcaatatg caaaggacat tgaattgtgt gaacgttttg
     8761 gtgtaaatgg tctaagatta tccattgctt ggagccgcat ttttcctgat ggagctggga
     8821 aaccaaatcc agaaggaatc gctttttatc atcgtgtgtt cgaagaatgt aagaaaagaa
     8881 acgtaacgcc gtttgtgacg ttgcatcatt ttgatacccc taaacgtttg tttgaccatg
     8941 gagatttttt gaatcgagaa acaatagaag catttgtttc ctatgccatt ttttgttttc
     9001 atgaattcaa agaagtcaag gtgtggagta cctttaatga aatctatcca gttgcaacca
     9061 atcaatattt gttaggtgtt ttcccaccag gaatcaaata tgattttaca aagatcgtcg
     9121 cctgtcttca taatatgatg gttgctcatg cccgtgtagt gaactatttc aaagaaaacg
     9181 aacttccggg tgaaattggt gtagttcact cattagagac gaaatatgcg gcaacagacg
     9241 cgccggaaga taagcatgct gcctttttag acgatgcatt gtcgatccgt ttcttattag
     9301 atgctaccta tttaggctat tattcaactg aaaccttgac tgctcttgat gagatatgtg
     9361 aagcaaatca ggccagctat cattttcctg aagaagattt cgttgaattg aaaaaagcat
     9421 ccacgcgtaa tgactactta gggatcaatc actaccaatg ccattttgtc aaagcttacg
     9481 atggagaaaa tgcaatccat cataacggta cgggtgaaaa agggacctct gtttataaag
     9541 tgaagggaat cggagaacgt atttataaag aaggaattcc tcgaactgat tgggactggc
     9601 tgatttatcc agaaggtcta tatgacttgt tgctgcgaat taaaagcgat tacccacatt
     9661 acaacaaaat ctatatcact gagaacggga tggggtataa agatcagttt gaagatggca
     9721 ttattatgga tcagccgaga atcgactatt tgagagtcta tttggaatcc ttaagcaagg
     9781 cgataacagc cggggtaaat gtgaaggggt attttttgtg gtcgcttatg gatcttttct
     9841 catggacaaa tgggtataac aaaaggtatg gcttgtttta tgttgatttt gaaacccaga
     9901 agcgttaccc aaaagaatca gcttactggt acaagttggt cagtgaaacc aaaacgatta
     9961 tttaataagt gataatggag gaagaaaaga tgaataaaga ggaattacaa atgcttggtt
    10021 ttgagatcgt tgcatattca ggcgacgctc gcagtacatt attgaaatta ttgaaagaag
    10081 taagacaagg aaactttgaa catgttgatt cggcgttgaa agaagccgac gaaaatttaa
    10141 cccttgcaca taattcgcaa acaaaaattt tagcggaaga agcatctggg aaagagatgg
    10201 agatgggatt catttttatc catgggcagg atcatttaat gaccacttta ttgctgagag
    10261 atctgattca agactttatt gtactgtaca gacagaatca aggataggag aagaacgcta
    10321 tggatggaat cattaaacaa atcgaaaaag ggaaaccatt ttttgaaaaa atctcacgta
    10381 atagttattt aggagccgtt cgtgacggat ttttaacagc gatgccagct attttatttt
    10441 caagcatttt cattttgatt gctgcggttc ctgagatttt tggttaccaa tggcctgaat
    10501 ctgtctccac atggctatgg aaagtctata attattcgat gggtgttgta gggttactcg
    10561 tttcagcaac cactgctcgc tgcttggctg attcgatgaa tcgtcgctta ccgaaaaaca
    10621 aagtaatcaa caccacctct gtgatgctgg cctctgtggt aagtttcctg ttgttaagcg
    10681 tctctccgat tgaaggtgga ttttcaacgg aatatatggg gacaaaggga ctactggctt
    10741 cctttgtcgc tgcattttta acagttaatt tatataagtt ttgtgtgctg aaagatatta
    10801 ccattaaaat gcctaaagaa gttcctggaa cgatttcgca aaccttccga gatatttttc
    10861 ctttctcttt ttcagtattt gcggccgttt taattgattt aggcattcga caaatcttcc
    10921 aaatctcatt tgcagaagca atcattacgg tgctgcagcc gctatttaca gcagctgacg
    10981 ggtacttagg aatcgctatt atctggggag cgatggcgct gttttggttt gtcggtgttc
    11041 atggaccgtc gatcgtggag ccggcaattg cggcaattat ctatgccaat gtggaaacca
    11101 atctgcaatt gttcaattcc ggtgaacaag cttctaaagt actgacggtg ggtttaggga
    11161 attttgtagg aactatggga ggaactgggg caacattggt tgttcctttt atcttcctac
    11221 tctttgctaa gtctaagcag ttgaaggcag ttgggaaagc ttcctttatt ccagtaagtt
    11281 tcgctgtcaa cgaaccattg ctatttgcgg cacccatgat tttgaaccct tattttttta
    11341 ttccattttt gttaacacct gttgtaaacg tttgcctgtt caagtttttt gtcgatgtat
    11401 tgggcatgaa cagctttatg tacgttttgc cttgggcaac tccagcaccg atcggtttgg
    11461 ttctaggaac gggcatgggt atcttagctt ttgttctagc agcggttcta atcgtcgttg
    11521 acttcctgat ctatctacca ttttgcaaag catacgatgc tgtcttagta gagaaagaag
    11581 cacaaaaagc agaggtgatc gcaaccgagg aaggtgcttc tgccgatgta cgtgaggaag
    11641 tacctgtggc agcgagtcaa gttatcagcg atgcaaataa agaaatcaat gtgttggtac
    11701 tttgtgctgg cgcagggaca agtgggatgt tagctaatgc tttaaaaaaa ggtgcatctg
    11761 aatcgaaagc accgatcagt gcaatggcgg gtgcttatgg ttctcattac gaaatgctgc
    11821 aggattttga tatggtggtc ttagcaccgc aagtcaattc ttactatgat gatatcaaag
    11881 aagacaccga tcgcttagga atcaagctgg ttgcgacaaa aggagcagaa tatatcggat
    11941 taacgagaga tcctgaaaaa gctgttcagt ttattctgaa ccaatttcaa taaaggagga
    12001 gccataatga aaatgggaat ttggttgcgt aaagctgttt tatttgcagc tgtgttaggg
    12061 gctttggggg ctgtttcaaa aactgttgaa gcatctgcca gcgaatttat tcgcggagca
    12121 gatatttcga tcctgtcaga tatggaaaaa tctggtgcaa aatattatga agacgatatt
    12181 tcaaaagatg ccttagagat tttaaaaaat aatggtgtca actatgttcg actgcgtttg
    12241 tggcaggatc cttataatag taatggagaa agttatggag cagggacaaa tgatctggaa
    12301 acgacgattg accttgcaaa acgagcaaaa aatctgggac ttaaggtgct gctggatttt
    12361 cactacagtg atttttgggt tgatcctggg aaacagaatc ttcctaaagc atggcaaggt
    12421 ctgacctttg aagaaatgaa tacggcattg tacgactata caaagaacgt tctgtcggaa
    12481 atgaagcagc tggacgttta tcctgatatg gttcagatcg gcaatgaact gaatagcgga
    12541 atgctctggc cttatggcaa gtcttgggga gaaggcgggg gagaatttga tcgattggct
    12601 gcttttttga agtctggtat ccaagctgtt cgagacactc aacctaaaac aactccagta
    12661 atgctccact tagctgatgg tggcgataca ggagcattta cttggtggtt tgatgaaatc
    12721 acgagtcgag gtgtgtcatt tgatttaatt ggagtgtcgt attatccgta ttggcacggt
    12781 tcaatgaatg aacttcaaac caatatgaat gcgatcagcc aacggtatgg taaagaagtg
    12841 atcgtagttg aaacagctta tgggcataca acagcgaatg ccgatactat gccaaatgct
    12901 tttggtgaag ccgaagcagc tgcgggggga tatgaaccaa caccgacagg acaagcagag
    12961 tatttgctgg atcttgccga tcggattcag gcagtaccga ataatcgcgg ggcgggcttc
    13021 ttctattggg aacctctttg gtacaacgga aatgtgagtt gggcaacaca agcaggtatg
    13081 aattatctca gtgttcaatc cactatggga aacgaatggg acaaccaagc gatgtttgat
    13141 tttgctggaa acgccttgcc aagcttgcgt gcgtttaaac aagcaggggc gcaaataaat
    13201 ctagttaaaa atgcaagttt cgaaagcgat ggaccaacga ccagtccttc caattggcaa
    13261 acctggtttc aacaaggaac cgctaagggt acgatcaaaa cggaagcggg cacttttttt
    13321 ggggactaca aactaacttt ttggcaggat caagcatacg aggcatcagc ttatcaaaat
    13381 gttagtggca taagcaaagg aacttacaca ttgagtgcct atgtaatgtc tgggggagag
    13441 tttgatacta atcagttata tatcaaaaat tatggtggag cggaactaaa tactgcggtt
    13501 gttaaaacaa gtgaatggac aaaaattaca atcaaagaca ttccaattac aacaggtgtt
    13561 tgtgaaattg gggtttatag caaggcttca ggaaatgctt ggacgaacgt tgattttgtt
    13621 tcattggtga aacaatagac tgcggatcgt ttcgattcaa gatcggaggt tcccttcagc
    13681 cacatcgtta gcgtagaagg caggaatcga acctgcacct cgcctttgcc aaaaataacc
    13741 agaaacgatt tccagtcatt tttaccaaaa cgagtaaagc accagacttc atctacactt
    13801 tgtattatgc taaagagagt tcaacgattt cgtaagaagc aatgaaaaaa ttgcaaaaag
    13861 ttgtgcagat aacataaagt ttgtttaaga aagggtaacc ttagaattcc gtcgtgaata
    13921 tgatttgagc acttagatgg atagaaaaaa gttctcggaa aacagttcgt ttttcgagaa
    13981 cttcttttta tgataagaat gaaacaaatt aacttatatt atagtctctt ttggtaggga
    14041 accatcttat ttttaatgaa aatcaagcat tttagctatt gaagaatgtt gatttatagg
    14101 gattccccct ccatattatg ttggctcttt attcccactc cactgttgct ggtggtttag
    14161 aagtgatgtc atacacgata cggttgacgt gggctacttc gtttacgata cgtacagaga
    14221 ttttttgcaa gacttcccat gggatacggg caaagtcagc ggtcattccg tcgattgaag
    14281 ttacagcacg gataccgact gtgtagtcgt aagtacgtcc gtcacccata acaccgacag
    14341 agcggatccc tggaagaaca gtgaaatatt gccagatatc acggtcaagt ccagcagctg
    14401 cgatttcttc acgcaagata gcatcagatt cgcgtacgat ttctagcttg tcttcagtga
    14461 tttcgccaag aacacgaata ccaagaccag gaccagggaa tggctgacgc caaacgattg
    14521 aatcaggcat gcctaactga gtccctaatt cacggacttc gtctttgaat aatgtgttta
    14581 atggttcgat caattcaaat tgcatatctt ctggcagtcc acctacatta tggtgagatt
    14641 tgatcgtctg tgctgtttct gtaccagatt cgataacgtc agtatacaac gttccttgtg
    14701 ctaggaaaga gacgccttct tcaccagcaa gttttgttgc ttcgtcatca aaaacatata
    14761 cgaattcatt cccaatgatt ttacgttttt gttctgggtc agatacacct ttcaacttgt
    14821 ctaagaagcg atctcttgca ttgactttga tgatgttcaa gccgaatttt ccgcctagac
    14881 tttccattac ttgttccgct tcgcctttgc gtagcagacc gtggtcaaca aagatagatg
    14941 tcaattggtc accgatcgct ttttgtaaaa gaaccccaac gacactagaa tcaacgccac
    15001 ctgaaagacc aagcaagact tttttgtcac caacttgttc acggattttt tctacttgca
    15061 tatcgataaa gttatccatt gtccagttac cttcacagtg acaaacatca aaggcaaagt
    15121 ggcgcagcaa ttcgattccg tattcggaat gacgtacttc tgcatggaat tggataccat
    15181 aaaaattacg tgttgtgtct tggatagaag caatcggaca atctttgctt gttccgactg
    15241 tttcaaagcc ttcaggaact ttagttacca agtcaccatg gctcatccat actgtttgtt
    15301 gttttggtgt ttctttaaac aagacagctt gatcgcttgt tacttctaac aaggctttcc
    15361 catattcgcg gtttttagca ggctctacgg aaccgccaag tttgtacgtg atcaattgca
    15421 taccatagca aatccctaat acaggaatac ctaaattgaa gatctcagga tcgatcccaa
    15481 atgcattctc atcatataca ctgtttggtc cgccagaaag aatgattccc tttggattca
    15541 tcgcttttac ttcttctgca gtaatgcggt ggctcaataa ttcggaaaag actccaagat
    15601 cacgaatacg tcttgtgatc aattggttat actgactacc gtagtctaat acgataatct
    15661 tttcaacgtt cgtcatatcg gcaacgtttg tcaaatttat tacccctatc cctttaaatt
    15721 ttatctcaaa atagtcaaac tattttaaaa tgctaatatt ttcgcatgaa aatcgaatcg
    15781 atgatatgat tttcagtttt atggagaatt atatcggctc ttcctcgtgt aggcaagata
    15841 tattcctgta aatttttcaa attcacattt ttccatacat cttttgccat tttaaaggct
    15901 tcttcacgat ctccgatagc aaaacgataa taataattct ctggattttg gaaagccgta
    15961 tccagtaacg caccaaaacg atccaaatac catttttcga tcagcacggg atcggcatcg
    16021 acatagacag aaaaatcaaa aaagtcgctg acgtagattt gctgattcgc aggtaattga
    16081 agcgtattga tcccctcaac gatcaagata tcaggctgct ggatcaattc gtacttgtct
    16141 ggaatgatat catacacatc atgtgagtaa acaggtactt ttatctcttc ctgaccagat
    16201 tttacacggt tcaagaaatc gatcagcatc tccatatcat aactttcagg aaatccttta
    16261 cggtccatga ttccacgctc ttttaatata cgatttggat aaagaaatcc gtcagtcgtg
    16321 atcagctgaa cgtttcttcg tttgaaggta cgagacaaaa tcgtctgcag cagtctggct
    16381 gttgtacttt ttccaacggc tacacttccg gcgatcccga taataaaagg cggtacttct
    16441 acgtatttat gaagaaataa acctttactc aagaacaaag attcaaattc tttcatataa
    16501 agataaatca gatgagcaag aggtacatag atatctcgaa catcctgcat ggaaatgcgg
    16561 tcattcaaac ttttgatatt atctagttca tcttgtgtca aaggtgcttg cccattacga
    16621 taaaattctc gccattcttc tcttgccaca cggtagtagt tcattggatc attcatatta
    16681 gcctcctgcg ttctcccatc aatggaacta ttttaacaca aaataaacgc aaaccccacc
    16741 acttaagaga gattgccgat ctttttgtat aatctttata atagaaagaa acggagtctt
    16801 attcttgact tgcgcacgga tacataaaag gatagactgt tgtcatgaaa gaaatgagaa
    16861 ggatggaaga catgacaaat cattactatt ctgaacgccc agatacggcg cacgattttg
    16921 aacaatgggc ttttgagtta aaaggcagaa cctttcaatt tgtaacagat tctggtgtat
    16981 tttcacgtga aacagtggat tatggttcac gtgtcctgat tgatgctttc gattgggaag
    17041 agttacctga tgaaggaaag atcttagatg tgggatgcgg gtatggaccg attgggttag
    17101 ctttggcatt tgcgacacag cggtttgttg aaatggtcga cattaatgaa cgagcggtaa
    17161 gtctagcgca aggaaacgca aagcgtaacc aaatcgaaca tgttgatatc catcaatcga
    17221 atatctatga agcagttcat gaagaaacct atgcagctat cgtaagcaat ccgccgatcc
    17281 gtgctggaaa aaaagttgta catgaaattt tgacaggcgc atatcctcgg ttgaagaagg
    17341 gcggtacact gacaattgtg atccagaaga aacaaggagc gccgagtgcg caaaagaaaa
    17401 tggaagaaac atttggtaat gcagaaatcg tcacaaaaga taaaggatat tatatcatca
    17461 aaagtgtgaa agaataacta gctgaaaaat agtggagcga cgaaagagtg gaactgggcg
    17521 tgcatgtacg ggtacgtatc agttcttttt ttgttgagaa gtttttaaaa acaaagaact
    17581 attttttctt caaaaagaaa tagtaggaac agagtcgttg ttttttacga taaaataaat
    17641 aagatacaga acttgtatag aaggctgcca agtcggtaga taaaggaggt ttttatcaaa
    17701 aaacatactt tccttttttc tctattcttt tcttgggggt tttggtacac tgatagggaa
    17761 tttagataga agggagccaa taggatgaag ctgaccatca gagatatcgc agaaatggca
    17821 ggcgtatcag taacaactgt ctcacagatt ttgaataaca aaggaagcag atttagtgat
    17881 aagacaagaa aaaaagtgtt gtcgatcgtg aatgaacatc attataagcc cgattatttc
    17941 gcttcaaatg tgatcaatcg acattcaaag accatcggaa tgatcgtccc tgatgtcact
    18001 gatttcttct tctccaaact gatcgaaggt gtagagagct atttgaatcc tttgggctat
    18061 gtgatcatgt tatgcaattc tcgtcatagc caagaaaatg agatcaagta tttacaagag
    18121 ctttcccacc gctcagtgga cggaatcttg ctggccacac caaatatttt acctgagaaa
    18181 tatgcattag acagcggttt ttatcaaaaa atgccaatca ttttaatcga tcgcggattg
    18241 aatcgtcggg ataacggacg gcttatcgta aaggaatatg agggcgccta tcaagctgtt
    18301 tcgctgttta ttgaaaatgg ccatacgaag atcggcatgc tgaaggaaac cactggatac
    18361 tatcaattag aagaacgttt caacggatat cgtcatgcgc tgaaagatca tggcctgcct
    18421 tttaatggga aatttgttga acatggagag ttgactgtcc aaggtggata tactgcatca
    18481 aaaaaattac tcaaacataa agatatcacg gctatttttt gcgggaatga tgccatggcg
    18541 atcggatgct atcaggcgat cgatgaattg ggcaaaaaaa tcccagaaga tatctcagtc
    18601 ataggattcg atggattaaa gctgtcagaa tacatgattc cgcaattgac gacagtgcag
    18661 cagccaagtt ttgatattgg attttatgct gcacgtttcc tgatcgatac gatcgaattt
    18721 cctcaaagaa aagttccgaa caaagtgttc gaaacaaaat taattattcg tgagagcgta
    18781 aaagtattga caaagggcta actcggcggt atattgctta tgtaagcatt tacaaaggct
    18841 agttagtgct ttttttattt ttagtaaacc agtttactaa agtggtttac ctttcctaaa
    18901 aataatgtat aataccgaat gaagacaagg aggatcaggc aatgcgaatg gtggatctga
    18961 ttgaaaaaaa gagggatgga aacgagctgt cgaaagagga aatcgagtac atcgtcacga
    19021 actatacgaa tggaaagatt ccagattatc aagtcagtgc attattgatg gcaatttttt
    19081 atcaagacat gacaaatgaa gaaatcacga acttaacatt ggcgattgct aattctggtg
    19141 atgtcatcga tttgagttct cttgaaggaa taaaggtaga taagcattcg acaggtggcg
    19201 ttggtgacac gaccacattg attttagccc ccctagtagc tagtgtcgga gtgacagtgg
    19261 ctaaaatgtc tggacgcggt ctaggttaca caggtggtac actagacaag ctagaagcaa
    19321 ttcccggatt tcagatcgag ctttcggatg aagaatttgt acgcattgtt aatgaaagta
    19381 aagtagcggt aattggtcaa tcaggaaact tggcaccggc ggacaaaaaa ttgtatgcct
    19441 tgcgtgacgt cacagcgacc gttgattctc ttccgctgat tgccagttcg attatgagta
    19501 aaaaaatcgc ggcaggagca gatgcaatcg ttttggatgt gaccactgga gacggtgctt
    19561 tcatgaagaa catcgaagat gcaagacgtc tggcaaagac catgacgagc attgggaaac
    19621 tggctaaccg agaaacagtg gcggttatct cagatatgtc agaacctctt ggcgaagcta
    19681 tcggaaatag tctagaagtt gtcgaggcaa tcgaaacatt acaaggaaat ggtccagaag
    19741 atctagtaga aatgtgctac gccttaggaa gccaaatggt tgttcttgct ggcaaagcta
    19801 agacaaccga tgaagcaaga acgttgcttc aagaagctct ggaatcaggt aaagcgttag
    19861 caaaattcaa agaaatgatc caaaatcaag ggggagaccc agcgatcgtg gaacatccag
    19921 aacgaatttt gactgcacgc tacacaatgg agcttcctgc taaacaaagc ggagttgtgt
    19981 caaaaatcgt ggcaaatgaa ttaggaattg ctgcaatgat gctaggtgct gggcgtaaaa
    20041 ccaaagaaga agatattgat cacgctgttg gattgaaact gcataaaaaa attggtgaca
    20101 ctgtgacaaa aggtgagtca ctattgacga tctatagtaa cgacgaagaa atcacttctg
    20161 tcattgaatt attatataaa aatattgaaa ttggtgaatc ggcaatgaag ccaacgttga
    20221 ttcatgacat catcacagaa taaaggaaaa aagcggactt taccgtttta aagaaaaggg
    20281 gaaaagaaat ggaactaaac cgaatgatcg accatactat tttgaaagca gacgcatcta
    20341 aagaagctgt gatgcaaatt atcgaagaag caaaaaaata ccatttttat tctgtctgca
    20401 tcaatccagc atgggttgcg ctagcgaaag aacaattaca aggtgagcca gtagccgtat
    20461 gtacagtaat cgggtttcct ctaggagcaa acacacccga gacaaaagca tttgaaacaa
    20521 cagatgccat caataacggt gcagatgaag tcgatatggt catcaatatc ggtgctttga
    20581 aatcaaagga tgatcaacaa gtccaaaaag atatcgaagc agttgttgaa gcagcaaaag
    20641 atcgtgcgct tgtcaaagtt attattgaaa catcgttatt agatagagaa gaaatcatcc
    20701 gagcttgcga aatagcaaaa gctgcaggcg ctgattttgt caaaacatca actggtttct
    20761 caacaggcgg agcaaaggta gaggatgtcc gtttgatgcg tgaaactgta ggaccagaaa
    20821 tgggtgtcaa agcttccggc ggtatccata acgaagaaga agcaatggca atgatcgaag
    20881 ctggtgctac aagaatcgga acaagcgcag gagtagcaat cgtatctggt tcaactggtg
    20941 aaggctatta attttttcaa aggagaagct catggcagaa ctcaaacaag aatggatcaa
    21001 cacagctatt gaagcgttag ataaagccta tgttccttat tcccattttc cggtaggcgc
    21061 ttgtctcgta acggaatcag gcaagattta tcaaggcatc aacattgaga atgcatcatt
    21121 tggattaacg aattgtgccg agcgcacagc ttttttcaaa gcagtatctg aaggagaaaa
    21181 aacatttacg catctagtcg tagcggggca cacccccgat cctatctctc cttgtggtgc
    21241 atgtcgacaa gtaatggcag aattctgttc ccctgatatg ccggttacgc tagtaggaga
    21301 caatggtgtg acgaaggcaa cgaccgttcg tgcactgctg ccttatgcat ttactgaaaa
    21361 agatctataa ctcatgttca ttcagtcagc tgactgttga aaaataaaac aattatttta
    21421 ggaggcttta cagaaatgaa aaaagcaaaa ttatttggtt taggtgctgt cgcactagca
    21481 gcagggttat ttttaggtgc atgcggcaac aacggctcta ctgattcatc cggtggcaaa
    21541 agctcatcag acacaacaac tgcagcattg atcacagaca caggtggtgt agacgaccgt
    21601 tcattcaacc agtcagcttg ggaaggacta gaaaaatggg ggaaagatca aggactttca
    21661 cgaggaaatg atggcttcca atatttccaa tcttcaaatg aatcagatta catcccaaat
    21721 attgaccaag cattgaatgc tggattcaaa acgatttttg gtattggtta taaactaaaa
    21781 ccagcgatcc aagaacaagc aacaaacaat acaggtacga actttgttat tatcgatgat
    21841 gtcatcgatg gattagataa cgttgtatct gcaactttca aagacaacga agcttcttat
    21901 cttgcaggtg ttgcagcagc ttatacgact gaaacaaacg tagtcggctt tatcggcggt
    21961 gtaaaaggtg aagtaatcga tcgttttgat gctggattca aagccggtgt tgatgcaggt
    22021 gcgaaagaat taggaaaaga aatcaaagta ttgaatcaat atgctggtga cttctctgct
    22081 ccagataaag gacgttcaat tgctcaaggt atgtatgcac aaaacgcaga tatcatcttc
    22141 catgcttcag gcggtacagg taacggtgta ttccaagaag caaaatcatt gaacgaatca
    22201 ggcgacaaaa aagtttgggt tatcggtgtt gaccgcgacc aatcagacga aggcgaatac
    22261 acattaaacg gcgaaaagaa aaacttcaca ttgacatcta cactgaaagc agttggaaca
    22321 gtagtagaag atttggcaca aaaatcagcg gacggtaaat tccctggcgg cgaacacact
    22381 gtttatggat tgaaagaaga cggtgttggt ctaacagaag gacaactttc tgacgaagcg
    22441 aaaaaagcag ttgatgaagc aaaagaaaaa atcatttcag gtgacgtaaa agttccagaa
    22501 actcctgaag aaaactaaaa ccatttctat agccttgtta gacacatgtt tgacaaggct
    22561 atggtttgat tagaacaaga attgttcatt tatccaagct gccttgtgac agtttggata
    22621 agggaacaac tccctgaagt aatgtttgtt tcaaaagaag aacgctcact aagcgtgaat
    22681 cttagtcaat cgttgcccta gatagaaatc gttaggatgt gaaaaaaagg tggttcaaga
    22741 aaatattgtc atagaaatgc agaatattac caaagaattt ggaacattca aggcaaatga
    22801 caacatcaat ttacaagtaa aggcaggaga aattcatgcg ttgcttggag aaaacggtgc
    22861 tggcaaatct acattgatga acgtgctttc cggattatta gagccgacat cagggaaaat
    22921 tttgatgcgt gggaaagaag taaagatcac aagtccgacc aaagccaacc aattagggat
    22981 tgggatggtc catcagcact ttatgcttgt tgatgccttt actgtaacag aaaacatcgt
    23041 gttgggaagc gagcctagtc gcgcagggat gcttgaccat aaaaaagcac gaaaagagat
    23101 ccaaaaagtt tctgaacagt atggattgtc agtcaaccca gatgcttacg ttcgtgatat
    23161 ttcagttgga atggaacaac gggtagaaat tttaaaaaca ctttaccgag gagcagatgt
    23221 actgattttt gatgagccga cagctgtatt gacccctcaa gaaattgatg aattaatcgt
    23281 gatcatgaag gaattagtca aagaaggcaa gtcgatcatt ttgattacgc ataagttaga
    23341 tgaaatcaaa gcagtagctg accgctgtac agttatccgc cgtggaaaag gaatcggtac
    23401 agtcaacgtt aaagacgtaa cctcacaaca attagccgat atgatggttg gaagaacggt
    23461 ttcattcaaa acgatgaaaa aagaagcgaa gcctcaagaa gtcgttttgt ctattgaaaa
    23521 tctagtggtg aaagaaaatc gtggattaga agccgtgaaa aacttgaact tagaggttcg
    23581 tgctggcgaa gtacttggta tcgctggaat cgatggaaac gggcagtcgg agttgatcca
    23641 agctttgact ggtttgcgaa aggcagaaag cggacatatc aagctaaaag gggaagacat
    23701 cactaataaa aaaccccgaa aaatcactga acatggtgta ggacacgtgc cagaagaccg
    23761 tcataaatac gggttagtcc tagatatgac attatctgaa aatatcgcac tacaaaccta
    23821 tcatcaaaaa ccttacagta aaaacggcat gctgaattat tcagtaatga atgaacatgc
    23881 cagagaattg atcgaagaat atgatgttcg aacaacgaat gaacttgttc ccgcaaaagc
    23941 tttatcaggc ggaaatcagc aaaaagcaat tatcgctcgt gaagtcgacc gagatcctga
    24001 tctgttgatt gttgcaaatc caactcgtgg actggatgta ggtgcgatcg aatttattca
    24061 taaacgtctg atcgaacaaa gggacaaata caaagcagtg ttactgatca gtttcgaatt
    24121 agaagaaatt ttaaatgttt cggatcgtat tgctgttatc catgaaggag aaattgtcgg
    24181 tatcgttgat ccgaaagaaa catctgaaaa tgaattaggt ctattgatgg cgggctatac
    24241 cttagaagaa gcacgaaaag agttagaaaa gggggctggc gcacatgaat gatcgcagcg
    24301 aaaaattccg taatttatta gtgcctgtac tttcagttgt tttaggattg atcttaggcg
    24361 cgatattgat ggctgctttt ggttttcagc ccatccaagg ttattcttct atgctagatg
    24421 cagcccttgg ttcgcaaaga gggataggtg aaacgttaag gcaagcaacg ccgttgattt
    24481 ttacagcatt aggtttttct gtagctaact cagctgggtt tttcaatatt gggctttctg
    24541 gacaagcact ttgcgggtgg atcgtcagtg tctgggtggc acttgcgttt ccagatctgc
    24601 caaaagttgt tttgcttccg ttatgcgtga tcttaggtgc cttagctggt gcgatggcag
    24661 cggctgttcc aggtttactt cgagcatttt ttggaacaag tgaagtgatc gtaacaatca
    24721 tgatgaacta tatcttatta tacgtaagca catttgcttt acatgaagtg atgcctgaaa
    24781 gctatcgctc aagtttggac tcttccaata tgatcagtca aaatgcctca ctgcggttac
    24841 catggttaac acaagctttt ggcggctctc gcgtaaactc aggattattt ttagcaatca
    24901 tcgcgcttat tttcgtttgg ctgctgatga agaaaacgac tttaggattt gaaatccgtt
    24961 ctgttggatt gaatcctttt gcttctgagt atgcaggaat gagtagtaaa cgtacgatcg
    25021 ttttgtcgat ggtcatttct ggcgctttgg cagggcttgg cggtgtggtt gaaggattag
    25081 gaacgtatca aaacttcttt gttcaaacga cttcgctatc tattggattt gacggaatgg
    25141 ctgtttcctt gcttggttct ggatcagcaa tcggtattct tttatcagcg atcttgttca
    25201 gtattttgaa gatcggtgga cttggcatgc agacaggagc aggtgttccg tttgagatcg
    25261 tcaatgtatc gattgctttg attatcttct ttgtcgggat caacttcctg attcgtttcg
    25321 tcatggctaa agtcttccaa gggaaaaaac aagaagagat cgttgcgaca atagaatcga
    25381 aaccaacgaa tcaaaaccat gaagggggcg aactataatg tcgactatcg aattaattgc
    25441 cttgatcctt acttctacac tggtttattc aacaccgcta attctaacgt cactaggcgg
    25501 aactttttct gaacgaagcg ggatcgtaaa cgtagggcta gagggaatca tggtcatggg
    25561 tgcttttagt tccatcgttt tcaacttgac gatgagtcca tcacttggtt ctatgacacc
    25621 atggatcgct attttagtgg gtggcgtcgt aggtatccta ttttctctga ttcacgcagt
    25681 agcaacagtc agtttgcgtg ctgaccatat tatcagcggg acagtaatta atctgatggc
    25741 acctgcttta ggtgttttct tgatcaaagc atggtatggt aaaggtcaaa ctgataacat
    25801 cacgcagaat ctggggtatt tttctttccc aggattagca gatattccgg tcattggacc
    25861 aattttcttt aaaaatacat tcttacctgc ttattgtgct attttgattg ctgtcctagc
    25921 ttgggtgatc atcttcaaga cgaaattcgg cttgcgcctc cgttcagttg gtgaaaatcc
    25981 tcaagctgcc gacacattag gtatcaatgt atatgctatg cgttattcag gcgtactgat
    26041 ttctggcttg ttaggcggta tgggcggtgc tgtttttgct cagtcgattt ccggtaactt
    26101 ttcagctggt acgatcgttg gacaagggtt catctccatg gctgctatga tctttggtaa
    26161 gtggaatcct ttaggggcaa tgggtgcctc attgttcttt ggttttgctc aaagcttgag
    26221 tatcattggc gcacaattac cagttatttc ttcgattccg ccagtattgc tgcaaattgc
    26281 tccatatttg ctgacaatca ttgtgctggt tgttttctta gggaaagctt ctggtccaaa
    26341 agcaaacggg aagaactata tcaaatcaaa ataatctttt agcgaataaa aagggtgggt
    26401 tacgggaagt gtttttcgtc tcgccctttt tgttttaaag gcaataaaaa taaaaagaaa
    26461 gaaggaataa tcatgtttaa acgcgtacat ctgatcgtca tggattcagt aggaattgga
    26521 gaagcaccag atgccgataa gttcggtgat gttggttctg atacattagg acacattgcg
    26581 aaagaagcag gattgaacgt tccacattta gagcagcttg gtttaggaac gatccgtcct
    26641 cttgaaggtg tggaatcagt gaaagatcat caaggctatg cgacgaaatt agaagaagtt
    26701 tctgtaggga aagatacgat gacaggccac tgggaaatca tgggattgaa tatcaaaact
    26761 ccatttcgtg tgtttcctaa tggattccct gatgatctga tcaaacaaat cgaagaattt
    26821 tctggccgta aagtcgtatg taacaaacct tatagcggaa cagctgttat tgacgattac
    26881 ggtgaacacc aaatgaaaac aggcgattta attgtatata cttctgccga tcctgtcctt
    26941 cagatcgcgg cacatgaaga aatcattcct ttagaagaat tatatcggat ttgccaattc
    27001 gttcgtgata taacaaaaga tgatccgtat atgattggcc gaatcattgc acgtccgtac
    27061 gtgggtgaac caggagactt cacacgaaca agcaatcgcc atgattatgc gttgaatcca
    27121 ttcggacaca cagtccttga ttctttgaaa gaagcaggaa aggacgtgat tgctgttgga
    27181 aagatcaacg atattttcaa cggacaagga atcactgaat ctgtccgcac gaaaagcaat
    27241 atggatggcg ttgaccagct gctgaaagtt atgaaaaacg atttttcagg aatcagcttt
    27301 accaatttag tagattttga tgcattatac ggtcatcgtc gggatgtagt cggctatgcg
    27361 catgcgattg aagaatttga cttacgaatt ccagaattgc ttgaagcgat ggaagaagac
    27421 gacttgctaa tgattacagc cgatcacgga aacgatccga cgtttactgg taccgatcat
    27481 acgcgagaat atgtaccgtt attggtttac agtaaaaaaa tgaaagaaca aggggcattg
    27541 cctcaaggat attattcaga tatttcagca acgattgctg agaactttag tgtttcttcg
    27601 actgaaaatg gcgaaagctt cttgaaatta ttaaaataat ctttttaaat aggagaaagc
    27661 aaatgaccaa gttaagcgag atgttacaag aaacgaccca gtttatcctt gataaaggag
    27721 taaaagaaat tgattttggt ttgattctag gttcaggatt aggtgaatta gcagatgaaa
    27781 tcgaagatgc tgtaatgatt tcttatgatg agatcccgtt ctttcctgtt tcgacagtag
    27841 ttgggcatgc aggaaagcta gtttatgggt ctctggcagg caagaaagtg ttagccatgc
    27901 aagggagatt ccactactat gaaggtcatt ccatgcaaac agtgacctat ccagtacgtg
    27961 tgatggccgg actaggggcc cattcagtca ttgtgactaa tgcatgtggt ggtgtcaacg
    28021 aatcatttgt accaggagat ttgatgctga ttaccgatca tatcaatttt actggtgaaa
    28081 atcctttgat cggtccgaat gaagatgcaa tgggccctag atttccagat atgagtcaag
    28141 cttatacgcc tgactatcaa gcaacagcaa aagcagcagc agaaaaacta ggtttggatc
    28201 ttaaagaagg cgtatatatg ggctattctg gaccaactta tgaaacgcct gcggagatcc
    28261 gaatgtctcg gacaatagga gccgatgcag tagggatgtc gactgttcca gaagtcatcg
    28321 tagcagctca cagcggatta aaagtgttag gaatctcttg tattaccaac ttagcagcag
    28381 gtatgcaagc gaatctgagc catgaagaag ttgtagaaac aactcagaga gtcaaacaat
    28441 cttttaaagc attggttaaa gaaacattga cattattgta ataggaggaa caataaaatg
    28501 agtgtgcata ttgaagcgaa agaaggcgag atcgccgata agattttact tccgggagat
    28561 cctttgcgag ctaaatatat tgcggagact tttttggaag atccagtttg ttacaatcag
    28621 gtacgcggta tgttaggctt taccggcaaa tataaaggag aacgtatttc tgttcaagga
    28681 acaggaatgg gaatgccatc agccaccatc tatgcccatg aattgatcca gtcgtatggt
    28741 gtaaagaaat tgattcgagt aggtacatgc ggtgcattgt caaaagacgt ccatgttcgt
    28801 gatttagtac tagcacaagg tgcagcgacc agttcttcga tgatcgaaaa aaatttccaa
    28861 gcctttcatt ttcctccaat cagtgatttc aatctattac tgaaagcata tgagatagct
    28921 aaagaaaagg gctatacggt tcatgtagga aatgttcttt cggaagattc tttctacaaa
    28981 gatgatttga cagagacgtt ccaattagct gaacttggcg ttttaggagt ggaaatggaa
    29041 gcagcggctc tttattactt aggcgcgaaa taccatgtgc agacattaag tttgatgacc
    29101 gttagcgacc atttgatcac cggtgaagaa acaacagctg cagaacgtca atctacgttc
    29161 aatgaaatga tcgaagtagg attggaaaca gcgatagcag aataagaagg aaatgaaaaa
    29221 gctgccctgc attaagtaat aagaacatcc aagcaaaaat agctcctcat gtttttgact
    29281 tggctggact tattattgca aatgtaagct tttgaacacc gtttaccaag aggttgtgaa
    29341 tctgccgttt aactctgagt cacaaggaac aatttcaaaa aacagctttt catgtttgat
    29401 gaatattgtg acttgtgacc gagaagttgg caggagaaaa ccgtttatac gaaaaaaggg
    29461 actgtgacaa atcgtgtcac agtccctttt ttatagtgtt ttttataggt agtatttttt
    29521 agcaactgtt aagtcatcat ttaattcata aacaagtggt tgacctgttg gaatttcaag
    29581 atccatgatg tcttcgtcag agataccttc gatatgttta gctagggcgc gtaatgagtt
    29641 accatgtgca gctactaaaa cagttttgtt gtctagtaat gctggagcaa tttcatcttg
    29701 ccagaatggc aaagcgcgtt ctaatgtcac tttcaagttt tctccaccag gaatatcgcg
    29761 tttgtctaac attgcgtaac gacggtcatt tgctgcagaa ccttcgtcag tttcttccat
    29821 taatggagga agagtatcgt aagaacgacg ccagatgtgc acttgttcgt cgccgtattt
    29881 ttcagctgtt tcttttttgt tcaaaccttg taatttacca tagtgacgtt cgtttaagcg
    29941 ccatgatttg atttgtggta cccaaagttg atctgaatat tctaagatca agttacatgt
    30001 tttgattgca cgagttaaaa cagaagtgta agcaacatca aattcgattc cagcttcttt
    30061 gattttgcgt ccgccttctt ttgcttcttc gattccttct ggtgctagat ttacatcagc
    30121 ccaaccagta aattggttca atgcattcca ttcactaaga ccgtgacgag aaaaaactaa
    30181 ttttggcatg tttagctctc cttttaagat aggtttatat ttctgaaatt ttcagttctg
    30241 ttttattgta caattttttg gaagagattt ccatcgaaaa attagataag attctatgag
    30301 aaaacaagta gagaatataa aaaaacgaac agtgcccacg caactgttcg tttttaggta
    30361 ttagatgcat aattgctcga tttctttttc aggtaaattg atatatttat aacatgttcc
    30421 aactgaaaca ccgcattttg aagaaataaa ttggatcgtt tctttttttt cataatagag
    30481 acgtcggatt tttttgatcg tttttgcatc gatttttggt cttccgccga tttttccttt
    30541 tttacgagct tcatctaatc caactaatgt acgttctttg atcaatgcgc actccatgtt
    30601 tgctaggttt tccattaatt ggaaatagat ttttcccatt tcgcgcgtat cgattccttc
    30661 atcaagacta atcaagtgca gcccgcgatc tttgaataat tgcgtgagtt ctgttaattg
    30721 ccgcgtagat ttgcctaatc gatgaagttg gcaaatgact agtgcatcgc cttgttgcat
    30781 gtcagcgatc actgactcca aatgaacagc agtagaatca ttttgttcca cttcatatgt
    30841 ctcatggaaa atttcttgac aaccaaagtc agataattga tcaacctgag ttgttaaatc
    30901 aacgtcattt atggttgtgc gtgcataacc aataattttc atggtgattc ctttctagca
    30961 gtgattttct aactggatat aacagtaaaa atgtagcata aagtacgtca aaaagatagt
    31021 tttttcgcaa attttccgct gtcttttttt attggatgat caagcagttt ttctttattt
    31081 acgacggatt gcgtcaagaa tctcaacgcc tttttctgag ccgacaacta ctttagaaac
    31141 catgtttagg aaaagcccat gttctactac cccaacaagt tcatctaaat aggtagctaa
    31201 gctttctggc tgttcgatga cttctagatg cagatcaatg atgtaatgac caccatctgt
    31261 ggttaagact tctccgtttt cgtctgtacg caagacgggt tggaatcttt tttcttcaaa
    31321 aatatgaagt agctgttggg agccataagg gatcacttca actgggagtg ggaacttccc
    31381 aagtttatcg actaatttag aagaatcaac gatccaaatc gtttcttttg agtacgtagc
    31441 gactattttc tcgaaaagca aagcagcgcc gccgcctttg atcccttgaa aatcagcact
    31501 gatttcatca gcaccgtcta ttgtcaaatc gactacgggg acttcatcaa tactctttaa
    31561 cggtattcct aatttttccg cttgttcttt tgtcgcattt gaagtagtaa ctcctgtgat
    31621 acgtaaacct tcttcacgca tacggcgacc aatctcttct accatataat aggcggttga
    31681 accggtcccg agacctacga tcatcccgtc ctttacgaat ttcgcggctt caatgccagc
    31741 taattgtttt aaattcatct ttgcgtccac ctttttttag tttgagcatc gtgctttcct
    31801 cactatttta gcgtgattta cggtaaaaag ataccattct tttttattcc aataaagctt
    31861 tttcaaaaaa aattgcatct cttgttgaca tgttttttca ttcgtgatat tctaacaaag
    31921 gtgctttatc tacaggtttc tgtgaagaaa cgtgctgact gtttatcaaa gcatatttga
    31981 taaggatgca gacattgcat ttatttttta tcacaaaaaa agaaccacct ggatgtgtgg
    32041 aactagacgc aaatcgtggg aaggaggaaa caaggaatgc ctacaattaa ccaattagta
    32101 cgtaaacctc gtaaatcaaa ggtggaaaaa tctaattcac ctgctttgaa caaaggatat
    32161 aacagcttta agaaaactca aactaacgta aactcacctc aaaaacgtgg ggtagcaact
    32221 cgtgtgggta caatgacacc taaaaaaccg aactcagctt tacgtaaata tgcacgtgtt
    32281 cgtttgtcaa acttaattga agttactgct tatatcccag ggattggtca taacttacaa
    32341 gaacacagtg ttgtattatt acgcggtggt cgtgtaaaag acttaccagg ggtacgttac
    32401 catatcgttc gcggtgcttt agatactgct ggtgttaacg accgtaaaca aagccgttct
    32461 aaatacggaa ctaaaaaacc taaagcttaa ttttaattat taaacaaatg aaacttgctt
    32521 ctactatata gaagtaatta ctatgaacaa tatttcggaa ggaggagtta cggatgccac
    32581 gtaaaggtcc tgttgcaaaa cgtgatgttt taccagatcc tatttataac tcaaaattag
    32641 taactcgctt gatcaaccgt gtaatggttg acggaaaacg cgggattgca gctaacatta
    32701 tctataatgc ctttgacttg atcaaagaat ctacaggtaa cgatccatta gaagtgtttg
    32761 aacaagcaat gaaaaacgtt caacctgttc ttgaagttaa agcacgtcgt gttgggggtt
    32821 caaactacca agtaccagtt gaagttcgcc ctgaacgccg cgttactcta ggtcttcgtt
    32881 gggtagtaaa ctacgctcgc ttgcgcggtg aacacacaat ggaagaacgc ttagcaaaag
    32941 aaatcatgga tgctgcaaac aacacaggtg cttctgttaa aaaacgtgaa gacactcata
    33001 aaatggctga tgccaaccgt gcgtttgctc actatcgctg gtaagattct ctctgtttgg
    33061 tcggctttta taagtcgacc tcagatagtg tccttatttt tagcaactag atagtaaaga
    33121 gaggagaaac aaggagaatg accagagaat tttctttaca aaacactcgt aatatcggta
    33181 tcatggccca catcgatgct ggtaaaacga ctgctactga acgtatcttg tactatactg
    33241 gtaaaatcca taaaattggt gaaactcatg aaggagcttc acaaatggac tggatggaac
    33301 aagaacaaga acgtggtatt acgatcactt ctgctgctac tacagcacaa tggaagggtc
    33361 accgaatcaa catcatcgac acaccaggac acgtagactt cacagttgaa gttgaacgtt
    33421 cattgcgtgt acttgatggt gctgtaactg ttcttgatgc acaatcaggt gttgaacctc
    33481 aaactgaaac agtttggcgt caagcaacaa cttatggtgt acctcgtatc gtatttgcaa
    33541 acaaaatgga caagatcggt gctgacttct tatattcagt atcaacttta catgaccgtt
    33601 tgcaagcaaa cgctcaccca atccaattac caattggtgc ggaagacgac ttcacaggaa
    33661 ttatcgacct tgtaacaatg aaggctgaaa tgtatacaaa cgatcttgga acagagatcg
    33721 aagaaacaga aattcctgaa gaataccgcg aattagctga agaatggcgc gaaaaattgg
    33781 ttgaagctgt agctgaaact gatgaagaac ttactttgaa atacttagaa ggtgaagaaa
    33841 tcactgaagc tgagttaaaa gaaggtatcc gccgcgcaac agttaacgta gaattctacc
    33901 cagtactatg tggttcagca tttaagaaca aaggggttca attactattg gacgctgttc
    33961 ttgattactt gccatcaccg ttagatatcc cagctatcaa agggattgac cctaaaactg
    34021 atgaagaagt agaacgtcct gcagacgaca gcgctccttt ctcagcttta gcatttaaag
    34081 ttatgactga cccattcgta ggtcgtttaa ctttcttccg tgtgtactct ggtgttttac
    34141 aatcaggttc atacgtacaa aacgcaacaa aaggcaaacg tgaacgtgtt ggtcgtatcc
    34201 tacagatgca tgccaactct cgttcagaaa tctctgaagt ttacgctgga gatatcgctg
    34261 ctgctgttgg tttgaaagac acaactactg gggacacact atgtgatgaa aagagtctag
    34321 ttattcttga atccatggaa ttcccagaac cagttatcca agtcgctatt gaacctaaat
    34381 caaaagctga ccaagataaa atgggtgtgg ctttacaaaa actatctgaa gaagatccta
    34441 ctttccgtgc tgaaacaaac gtggaaacag gcgaaacaat catcgctggt atgggtgaac
    34501 ttcacttaga tatcatcgtt gaccgtatgc gtcgtgaatt caaagttgaa gctaacgtag
    34561 gtgcgcctca agtatcttac cgtgaaactt tccgtgctgg cactcaagcc gaaggtaagt
    34621 ttgtacgtca gtctggtggt aaaggtcaat acggtcacgt atggattgaa tttacaccaa
    34681 atgaagaagg tgctggtttc gaatttgaaa atgcgatcgt cggtggggtt gttcctcgtg
    34741 aatacattcc agctgttgaa actggtttga aagatgctat ggaaaatggt gtcttagctg
    34801 gctatccatt agtagatatc aaagctaaac tttacgatgg ttcataccat gatgtcgatt
    34861 caaatgaaac tgccttccgt gttgctgctt ctatggcatt acgtgcagca gctaagaaag
    34921 ctaatccagt aatccttgaa ccaatcatgg ctgtagaagt tgttatccct gaagattatt
    34981 taggtgatgt tatgggtcac gtaactgctc gtcgtggacg tgttgaaggt atggaagctc
    35041 gcgctggcgg ccaacaagtt gttcgtgcga tggttccact tgctgaaatg tttggttatg
    35101 ctacaactct tcgttctgct actcaaggtc gtggtacatt tacaatgaca tttgaccact
    35161 acgaagatgt accaaaatca gtacaagaag aaatcattaa gaaaaacggc ggaaaagctg
    35221 aataatttct tgaaaccgga tagtcctgca aagagattat ccaaagagac tatattttta
    35281 atatagtttc gtgtaaaata attatcgatg atatgggcat gtggtgtacc ttgtgtacat
    35341 catataccta tcagacaaat aaaaaatata ttgtattttt aggaggacat tttaaaatgg
    35401 caaaagaaaa atttgaccgt tctaaaccac acgttaacat tggtactatc ggacacgttg
    35461 accatggtaa aactacatta acagctgcaa tcacaactgt actatctaag aaaaacggcg
    35521 gccaagctat ggcttacgat caaatcgatg gtgctccaga agaacgcgaa cgcggaatca
    35581 caatctcaac agctcacgtt gagtatgaaa cagatacacg ccactacgct cacgttgact
    35641 gcccaggaca cgcggattac gttaaaaaca tgatcactgg tgctgctcaa atggacggag
    35701 ctatcttggt agtttctgct gctgacggcc caatgcctca aactcgtgaa cacatcctat
    35761 tgtctcgtca agttggtgtt ccttacatcg ttgtattctt gaacaaagta gacatggttg
    35821 atgacgaaga attactagaa ttagttgaaa tggaagttcg tgacctatta acagaatacg
    35881 aattccctgg tgacgatgtt cctgtagttg ctggatcagc tttgaaagct ctagaaggcg
    35941 acgcttcata cgaagaaaaa attcttgaat taatggctgc agttgacgaa tacatcccaa
    36001 ctccagaacg tgacaacgac aaaccattca tgatgccagt tgaagacgtg ttctcaatta
    36061 ctggacgtgg tactgttgct acaggtcgtg ttgaacgtgg acaagttcgc gttggtgacg
    36121 aagttgaagt tgttggtatt gctgaagaaa cttcaaaaac aacagttact ggtgttgaaa
    36181 tgttccgtaa attgttagac tacgctgaag ctggagacaa cattggtgct ttactacgtg
    36241 gtgttgcacg tgaagacatc caacgtggac aagttttagc taaaccaggt acaatcacac
    36301 ctcatacaaa attctctgca gaagtatacg tgttgacaaa agaagaaggt ggacgtcata
    36361 ctccattctt cactaactac cgtccacaat tctacttccg tacaactgac gtaacaggtg
    36421 ttgttgaatt accagaagga actgaaatgg ttatgcctgg cgacaacgta acaatggaag
    36481 ttgaattaat ccacccaatc gctatcgaaa acggtactaa attctcaatc cgtgaaggcg
    36541 gacgtacagt tggtgccggt gtcgttacag aaatcaaagc ttaattcact taagttttaa
    36601 agtaaagcaa catttattcg gacgtaatcg ctcggaacga aggtcttagg atctttgttc
    36661 cgagcttttt tatttattgc aggatcagga aaaaagacag gagtattata aatagaagaa
    36721 acacgaaata aaagagtcag tattttctaa atgaaacttt atgaaaacaa aatgtttttt
    36781 acaagaaaaa g
//
LOCUS       DAZKLA010000016        36517 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000016 DAZKLA010000000
VERSION     DAZKLA010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.016"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            321..641
                     /locus_tag="ACG85N_000646"
     CDS             321..641
                     /locus_tag="ACG85N_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFQ0223442.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            795..1700
                     /locus_tag="ACG85N_000647"
     CDS             795..1700
                     /locus_tag="ACG85N_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0223443.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1697..2470
                     /locus_tag="ACG85N_000648"
     CDS             1697..2470
                     /locus_tag="ACG85N_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0223444.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2651..4075)
                     /locus_tag="ACG85N_000649"
     CDS             complement(2651..4075)
                     /locus_tag="ACG85N_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFQ0223445.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4286..4732
                     /gene="nrdI"
                     /locus_tag="ACG85N_000650"
     CDS             4286..4732
                     /gene="nrdI"
                     /locus_tag="ACG85N_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0223446.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4797..6779)
                     /locus_tag="ACG85N_000651"
     CDS             complement(4797..6779)
                     /locus_tag="ACG85N_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0223447.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6793..7689)
                     /gene="murQ"
                     /locus_tag="ACG85N_000652"
     CDS             complement(6793..7689)
                     /gene="murQ"
                     /locus_tag="ACG85N_000652"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0223448.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7692..8753)
                     /locus_tag="ACG85N_000653"
     CDS             complement(7692..8753)
                     /locus_tag="ACG85N_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0223449.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8964..9491)
                     /locus_tag="ACG85N_000654"
     CDS             complement(8964..9491)
                     /locus_tag="ACG85N_000654"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0223450.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9558..11060)
                     /gene="eat(A)"
                     /locus_tag="ACG85N_000655"
     CDS             complement(9558..11060)
                     /gene="eat(A)"
                     /locus_tag="ACG85N_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0223451.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11318..12439)
                     /locus_tag="ACG85N_000656"
     CDS             complement(11318..12439)
                     /locus_tag="ACG85N_000656"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0223452.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12565..13506
                     /locus_tag="ACG85N_000657"
     CDS             12565..13506
                     /locus_tag="ACG85N_000657"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0223453.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13656..14552)
                     /locus_tag="ACG85N_000658"
     CDS             complement(13656..14552)
                     /locus_tag="ACG85N_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0223454.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14821..16161
                     /locus_tag="ACG85N_000659"
     CDS             14821..16161
                     /locus_tag="ACG85N_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0223455.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16379..16822
                     /locus_tag="ACG85N_000660"
     CDS             16379..16822
                     /locus_tag="ACG85N_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFQ0223456.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17326..18744)
                     /gene="nhaC"
                     /locus_tag="ACG85N_000661"
     CDS             complement(17326..18744)
                     /gene="nhaC"
                     /locus_tag="ACG85N_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0223457.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19147..20370)
                     /locus_tag="ACG85N_000662"
     CDS             complement(19147..20370)
                     /locus_tag="ACG85N_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0223458.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20401..21240)
                     /locus_tag="ACG85N_000663"
     CDS             complement(20401..21240)
                     /locus_tag="ACG85N_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0223459.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21257..21937)
                     /locus_tag="ACG85N_000664"
     CDS             complement(21257..21937)
                     /locus_tag="ACG85N_000664"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0223460.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21939..23012)
                     /locus_tag="ACG85N_000665"
     CDS             complement(21939..23012)
                     /locus_tag="ACG85N_000665"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0223461.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23068..23306)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23349..23582)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23659..24330)
                     /locus_tag="ACG85N_000666"
     CDS             complement(23659..24330)
                     /locus_tag="ACG85N_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0223462.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24417..27065)
                     /gene="mgtA"
                     /locus_tag="ACG85N_000667"
     CDS             complement(24417..27065)
                     /gene="mgtA"
                     /locus_tag="ACG85N_000667"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFQ0223463.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(27407..27581)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            27754..28191
                     /gene="ndk"
                     /locus_tag="ACG85N_000668"
     CDS             27754..28191
                     /gene="ndk"
                     /locus_tag="ACG85N_000668"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFQ0223464.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28213..29079)
                     /locus_tag="ACG85N_000669"
     CDS             complement(28213..29079)
                     /locus_tag="ACG85N_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0223465.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29239..31332)
                     /locus_tag="ACG85N_000670"
     CDS             complement(29239..31332)
                     /locus_tag="ACG85N_000670"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0223466.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32081..32704)
                     /locus_tag="ACG85N_000671"
     CDS             complement(32081..32704)
                     /locus_tag="ACG85N_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223467.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32863..34260)
                     /locus_tag="ACG85N_000672"
     CDS             complement(32863..34260)
                     /locus_tag="ACG85N_000672"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0223468.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34292..35263)
                     /locus_tag="ACG85N_000673"
     CDS             complement(34292..35263)
                     /locus_tag="ACG85N_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0223469.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            35637..36062
                     /locus_tag="ACG85N_000674"
     CDS             35637..36062
                     /locus_tag="ACG85N_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223470.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT        10568 a         7607 c         6397 g        11945 t
ORIGIN      
        1 ttttttaatt tattgcccga taatatatag aaataaggtc tagttactgt tttttttaaa
       61 tgattaataa ataacaactt ctttctatga aattgcaaag cattcatctt cttttccgct
      121 aacgatatat ccgtcagtgc ttggtacact ttactaaaaa gtatgagatt gccgatatct
      181 ggataggtgt gctatactac agaaaaagat atacaatctg ccaccgggta ggataaagag
      241 gatgatcatt ccattaggtc tttgaaggga tgattttcct ctttttgtgt atttttttat
      301 agttaaggag gaatgtaagt atggcatgga tggaattaat catagcagga attttggaag
      361 tattctggtc aacgatgatg aaatggagtg atggtttttc taaaatcaat tattcttttt
      421 acacagtgat tggcatgatt gctagttttt actttttgtc aaaggcaatc aaaagtttgc
      481 cgatgagtct ctcttatcct atctggacag ggattggcgc agttggttcg gtcataattg
      541 gtgtggtctt gtttcatgat aagctgaatc tttccacttg gttctttgtg gggatgctac
      601 tcatcagtat cattggcatc aagattacta gcggccacta gacacttgga ataagtactg
      661 atcagaaaaa ataagccttt ttatttaata ttcaattttt ttatgaaaaa ataagaaata
      721 agtccaatga cctagcgact tctccactca tcaggtgtat gcttgtacta tctatagaaa
      781 aggagtgaat atttttgaaa agtctttcgg tccaagaatt atccaagaac tttggtcaca
      841 aacaagccct cagcgatgtc agttttagct tatcccctgg caaaatcgtt ggattagtag
      901 gtcctaatgg cgctggaaaa acaacgatca tgaaagctat tttaggactg atccattact
      961 cagatggaac aatctgtatt ggtgaaaatg agatttctcc ctctagtcat aaagggttgg
     1021 aacaagtagg agcattgatc gaatacccgg gtatctatcc attcttaaca ggctatgatc
     1081 atctgaagtt attctctgaa accaacgatg tatctgctat cgacactatc gtgaatcagc
     1141 tcaagatgga aaagtacatt cacaaaaaag cgaaatccta ctcgttggga atgaaacaaa
     1201 aattgggaat tgctcttgcc ttactgaacc atccgtctat cgttatttta gatgagccga
     1261 tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa aatcattacc gacttggcta
     1321 ctcagggcac ctctttcttg atctccagtc acttactcag cgagcttgaa aaaatggtcg
     1381 atgagctgat cctgatcaat caaggaaaga tcatcaaaca atgtacaatg gctgaattga
     1441 accaaacagc gcaagatttc atcgttatct ctacaacaaa tgatctagct gctcgaacga
     1501 tcttgacaga agctggatat actttggaag atcctgataa aatcaagctt ttaaaagaaa
     1561 acgaagaatt attaggaacc gtgatccagc tattgtctgc acaaaatatc catgtgacag
     1621 acgtccagca tctgacgaat gatttggaat cttctctatt ggaaattctg gattcagtaa
     1681 agggggaaga agaaaaatga cattgcttgt aaaacaagaa ttatttaaat tgatcaagaa
     1741 aaagtcaact gctgttttat ctgttctatt agttgttttg ctcataggaa cagcacttct
     1801 agccaaaaaa tacacgacga tcattgatcc agttgaaatg actgctcagc tttttagtgc
     1861 cacatcttgg attgtcttta tcatgattgc agctgcaagc acgatcattt cgatggaagc
     1921 acaatatggt acattgaaaa atctccttta tcgaaagtat tctcgaggag aaatattagt
     1981 aagcaaatgg atcacattag ttatttattc tgtttattta taccttttag ctattattgt
     2041 cactgtgttg atgaaactga ttcttttccc ttcgatttcc ttcacggaga aggtttctac
     2101 tggtcaaaca ctgatccaaa gcttgttcac ttatactttg ggtagttata tcggtttatg
     2161 gctgatttta agtttagtgt tgatgattgc ttgctttatc aatagttccg gcgcatccat
     2221 ctcagcagga atcgtctttt attttgcttc gtcaatcata tctggtatcc ttattgcact
     2281 catccaaaaa tgggagtgga tcaaatggaa tccgatcagt atgttgaatc tgcaaaacca
     2341 aattggaaac gaagagatca tgaagcaatt gactcatctt tctactaatc aaatgctatt
     2401 cggtaattta gcttacatcg ttttgttctt agcattagga tatctcgtat ttaagcgaaa
     2461 aaatgtatga tgactcatca aaaaagaggc tgtgaaagag acgttcagcc ttattgccga
     2521 ttggctgtct cttaaacacc gtttattctg aaaaaggagc tgtgacaaca tttgtgtcac
     2581 agctcctttc ctagtatgtt tttgaataaa cgacaggctc acaacctctc tttatacttc
     2641 ttttatactt ctatacgtga agcatcccag tggcaaataa aaccattgca atcactccga
     2701 ccatcgtcac acctataagt aaaatcttat ctaagtggat atgttgtaca ggctcgtttt
     2761 gctccttacg tgctttgagg taaaaataaa tgcctggcaa gaaaagaaca agaacaagca
     2821 ttacttcatt aaaggctacg actagcatag cccatacttg aaaggcactt gctaaaagcc
     2881 ctactaaaaa ttggacccat tctttccgct gataagaaag tttcatttgg tacaatccta
     2941 agaaaagcca cgtgaagaaa atagctgctg taccaagtgt gtaagcaaag ttataagcac
     3001 tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca taacgtcgtc aatacgatag
     3061 cataagttgg tgccccattt ttgttgagtt ttccccatgc tttaggcaaa agtccgtctt
     3121 cagccatgtt tcccattgtt tcaatcggta agatcgtcca tgagagccaa acaccaacta
     3181 aagaaatgat caaaccgccg ttgatcacga ctgctcccca tttaccaacc atcatttcaa
     3241 acacatagcc catagcaggc tgcgccttga tcgtttgcag ctgattcatc gtcattgtcc
     3301 catatggaag catggaaacc aagacgtaaa taataatcaa gccgaatatc cctagaatcg
     3361 ttgctttccc aacatctgat tgtttttttg ctcgtgaacc tacgacagag gctccttcaa
     3421 taccaacaaa tacccaaagc atcaccatga tgcaactcaa tacttgactg gaaaacggga
     3481 tcggctgtcc attcgcattc aatataatgt cgctagaaaa gttatcaatg aattgttgga
     3541 tcgaaaacgt gctgatagct actacaataa ataaaaataa tggcactaat ttacagaatg
     3601 tgacgatagc attgacgaaa ctagcatttt cgactccccg attcactagc catgcatatc
     3661 cccataaaaa gactgtactc atcacgatcg atgccagatt ttgcccacct tcaaatactg
     3721 ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa agctacgttg cctaaccagc
     3781 atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa acggccaaaa ccttcttctg
     3841 cgtaactaaa gatcccgcct tttaaatctg gacgtttttt attgatatta ttgatcgaca
     3901 aggagagcat caacattgct gttcctacga gtacccaact gaaaatagct gggccgccag
     3961 ccgatccgga gatgtccgtc ataataccaa atacgccacc tccaatcgct gaaccaatta
     4021 ctaaggcagc aagtggaatt aaaccaattc cttttttctg ttgctctgta tccatgtaag
     4081 catcctccaa aataaaaccg cttttatcaa aaagtataac aaaccttttt tatttttact
     4141 taattaataa aaactttaat agaaatataa ttctaccggc ttttttctca ttatacaagc
     4201 aaagcgccta gtccctttcc aaattacacc accaaaaaac ttaaaaaaga tgctatacta
     4261 aaaggaaaga attggaggat gatccatgaa cattttatat atatcgattt caggaaatac
     4321 gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa atgcatgcag aagatcctgt
     4381 caatcctgaa gttactttaa aagaaatcca tgaaaatagt gattttacga aagaaacaga
     4441 accatttttt actttcgttc ctacttattt agacggcgga aatggtttag ataacggtga
     4501 tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa catgaagaca atcataaact
     4561 ttgtcttggt gtagtcggta gcggaaataa aaacttcaat aatcagtatt gtctaacagc
     4621 caaacaatat gcacaaacat ttggctttcc tttcttggca gattatgaat tacggggaac
     4681 accatctgat gtgacacgaa tttatcagat tttagcagaa aacaataaat aataaaaaag
     4741 aagaagctga aaaatagatt tcagtttctt ctttcgtttc tattgtttct atcatattaa
     4801 gctttgacat ccatcacagg tgcttttgct tttgctttac ctgttttttc caagacaaag
     4861 ttttttacac gatccatatt ggttaccaag acgatcatgg atgtttcttt gcctgctgct
     4921 ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag gtgtcacttg ctgcccttct
     4981 gttactttca catcaaatgg cgtaccgcct aagtcaactg tgttgattcc catatgaagc
     5041 aagatttcta aaccatttgg cgttgtgatc ccaatagcgt gtttcgttgg gaagacagag
     5101 ctgactaccc cagtaactgg agaatagata tgtccgtttt ctggttctac aaagtagcct
     5161 tcacccatca tcttttgtgc aaaaactgga tcacttgctt tatctaaaga aacaactgtt
     5221 ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg aagtttgagg tactactttt
     5281 tcaggcactt catttactgt ggctgtttca gtcgctaatt cttctttttc taaacggtct
     5341 ttaggaatac cgaagaagaa tgtagcaacg aagccaccag cataagctgc caataatcca
     5401 aggatgtagc ctaaccattt accgtcagag atcaatggga tcaaagcggc tcctgaaggt
     5461 ccaatagcga tagccccgac atttccgatc attccgatca ctgccccgcc gatacctcca
     5521 ccgatacaag cagtaataaa tgggcgtcct agaggtaatg ttactccgta aatcaatggt
     5581 tcgccgattc ctaggatccc gactggtaaa gctcctttga tcatttctgt taattcttta
     5641 tcttttttgc agcgaatcca taatgccaat gctgcaccca cttgtcctgc accagccatc
     5701 gcaagaattg gaagcaatag cgtacgacca gtttcatcaa tcatttgcac atggattgga
     5761 gtcaaaattt gatgcagtcc gaacataacc atcggcaaga ataatgtgcc tagaacgaag
     5821 ccggcaaatg ctccaccaac gccaagtacc cagttgatcc caccgaccat gccatcagaa
     5881 atgaaacctg ctaaaggcat gatgaagaaa atttcaatca gaccgatggc aatcaatgaa
     5941 agagttggtg taacgatgat atctaccgca tcaggtacta ctttatgcag ttttttctca
     6001 acgattgaca atagccaaac agcgaagatg acacctagga tcccaccttg tccagctgca
     6061 agagcttcac cagtaaataa gtttttcagt ggtgcttccg gattcatccc tgtcagcatc
     6121 acaacggccc cgataactcc accaagagtt ggtgttgcac caaaaacttg tgcagcattg
     6181 atacctgtat agataactag gtagctaaac atcccgtttt tcaagatgtt catcacatcg
     6241 atatattgtt gccatgttgc tgcatcaagt gttccggctg tgactaagtt tgctaaaact
     6301 gcggcaatcc ctgccacgat cccagtaccg acaaatgcag gaatcattgg cacaaagata
     6361 ttagaaatgt cttttaatac cgatttgatt ttagatggtt tttgtttcga tttttgtgcc
     6421 gctttcattt cttgtgcccg ttcgtttaca cggtcttttc cagatgctga tgaatttgct
     6481 gtagctgtac ttacagaaga aagtttttct cctaatttca cgcctgccaa atcaaccatt
     6541 tgctgtgcta ctttgttcac tttcccaggt ccgatgatga cttgaagctg ttcatcttct
     6601 actacaccaa gcactcctgg aatcgctttt aattcagaga catttacaag atcatcattg
     6661 atgatcttca tacgcacacg agtcatgcag tggacaatgc tttctacatt gcccatgccg
     6721 cctgcccctt tataaatttg ttttgccagt tcttccaaag tcagttcgtt ttttgccatg
     6781 gtcttttccc cctcataagg tcccttttac aaatccgttt gcttcagcta attttgcttg
     6841 agcttcctct ttatcactat ttgtcaaaat catgacgata gccagcttaa cattttgatc
     6901 tgcttcttca aagtactttt cagctgtttc gtaatcagct tctgtcgctt gcatgatgat
     6961 tcgttttgag cgttcgatta gtttttcatt tgttggtttg acatcgacca tcaagttatt
     7021 gaatacttta cctataccaa tcatactgat agtagaaatc atattcaaga taagtttttg
     7081 tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat tctggacctg catcgacttc
     7141 gattgggaaa tccgcgtgtt tactgatttc ggcttgttta ttgcaagaaa tcgttcctgt
     7201 gacagcaccg atcgattttg cgtaatctaa tccaccgatc acatatggtg tacgaccgct
     7261 agctgcgata ccaataacta tatcattttt tgtcaaattc agcttgatta gatcttcttg
     7321 ccctagctca taagaatctt cggccccttc gacagctaca gtcattgctt cttctccacc
     7381 agcaattaga cctatgacca tttccggttc tacaccgaat gtcggtacgc attcagcagc
     7441 atctaacaca cctagtcttc cacttgttcc ggctcccata taaatcagac gtccaccttt
     7501 tttaaaggac tcaatcgtgt tagcaatcac tggttcgata cttgtcagtt ctttttcaac
     7561 cgcagcggcc actttctgat cttctttgtt cattagttga acggcttgag aaatactcat
     7621 ctcatctaat ccaaaagttt cttggtttct gcgttccgtt gttaggttct ctaaattgat
     7681 tttgctcatt tttatcttcc ttcttttctt ataaattgat agttcatacc agctttgatt
     7741 tggttgatca atggtagctc ttctttgata actttagcta ctacattcac tttttcatcc
     7801 gctggcaaat catatttggt taattggatc tcacccatat agcgcaaata cttttcgtta
     7861 tctaaagtaa tagacccttt catacgcgta gctgtatttc ttgctgggat aggcggaatc
     7921 tctcgaaaac gtgcatctac gcttcgtatg acatctcttg catcgtcttg ccgattcgta
     7981 tgcttcccta atatatattc gtaaaattct tcatctacag gttccgtatg gagcaagact
     8041 gtctgttctt tttggaaaga agcaaattgc tcctgaacgt tttctgacag acctgcatct
     8101 ccgatgtaaa caatatccgt gttacttgct aatagatcta gagcgcctgc aagaggatgg
     8161 atccctcgat gttcttccaa agttggcaat ccttgatata gtgggcctct caactctgta
     8221 tctcctggta caaaaccttg gacaaggaga ccttgaattt tgagccattg attttttttc
     8281 tgataccatt ctttgtccag ccctgtttcg ggacgtggat aataattatg ccaagcttca
     8341 atctgtgaga aatcagcttc tgcttctttc aattcatcga tatctttagg cgtgatcgtg
     8401 ctagcattca aactgatttt caattgatgt gaccagtcgg caatctgctg attggaaata
     8461 tgataatcca ttcttaaacc tgttacacca attgctttca aaggcgttag atcttctaat
     8521 gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga ccatcaattc tagtttattc
     8581 tttttcgctt cgcttcctag ttcgatcaat cgcttttgat atgccgaaac atcatcttct
     8641 ggtatatgca tggaagtgaa aataccgaca aagccattgg atgccatttg gcggatatac
     8701 tgtttcttct cttctgacaa agaatcgttc ataaatattg aaaagccaaa catattatcc
     8761 ctctttttct gtatccgtct tcatttctat gactagagta tatatctaaa gaaacaaaat
     8821 ttcaatactt ttattaaaat aattaaattt catttctaaa ggttgagttt atattatcaa
     8881 aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa gcagtgcctt gaaatatttt
     8941 atttaaataa cacaaaaaca taattactca taagaaacca attctgtatt atctaccatg
     9001 aatgtaaaga acccctcatt aggcatcgga tgaaaataag cattgatcac tcttatcaag
     9061 gcattatggc aaactaacag atagacattt tcttcgtctt cgatgcattc tttcaataaa
     9121 ggaacgatcc gtgcatacac atctaagact gactccccat ttgggaatcg tacagcgaac
     9181 gctaaacgtg ctttttgaaa gttctcatct ttgcttggca gtccatcata atccccaaaa
     9241 tccatttcta ccaatcgttc atcaacggtc agcggcaagc tgagacgatc agcaactgct
     9301 tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga ttttcgtgat tggaacaggc
     9361 aaatctacta ccttttctgc tagtttctgt gcctgcatct gtcctttttc agtcagcggc
     9421 acatctgccc gtccgcaaac taatccttgt gcgttccaag ttgtttctcc gtgtctcgtt
     9481 acataaagca tgtgattctc tccttcgttg gcaaagttcc ccttaaccat accttgttgt
     9541 tcttgagatt acaagggtta gattctctca agagaaataa tctcagtaga tactttatcc
     9601 aggaaggttt gatcatgttc cactagtaac atggcaggtt tcacgttcaa gatcagttgt
     9661 tctaattgtt cttgattgaa gacatccaaa taattcaatg gttcatccca tgtatatagt
     9721 tcagctggct gtgataaaga tttagccaat tccacttttt tccgttggcc catactcatc
     9781 tgctcgatct tgttatgaaa aacatctctt tccatcccaa gctttcggag gttgttcaaa
     9841 aatgcttggt agtctacttg gtttttctct gcaaattccg ccaacgttcc tcgattgtct
     9901 tcataatttt ggcttgcata actaatgctc agatgttttg gctggtattt ttctcctata
     9961 accttgccgt tgaatgcccc cagaagataa tggataatgg atgactttcc tgcaccgtta
    10021 ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa aatgaattgg ctcgaataac
    10081 agattttcat accctaattg aagatcttct acgcttaaaa gtcgcttatg gtgagacgct
    10141 tggctattca tcgtcaaaga atcgatatat tcgatatctt ttaatagttt ttctttctcg
    10201 ctgatctggg tctccatccg attcacgatt gctttggatc gtttcatcgt ccgtgcagca
    10261 tcagcaccca ctgctccttt attcactcgt ctagattcag tatcgatgaa tccgacttgt
    10321 ttctttgttt tatctccttc tcgggaacga gaccattcgg ctttttcagc tgctgttttc
    10381 tttagcctac tgacttcttt tttcaatttt tcattttgag ccatttcaaa ttcatcacga
    10441 agttttttct gttcttcata gatagagaaa ttcccttgat aaagttccag ttgacttttt
    10501 tcgattgcta aaacatggtc cactacttca tcgataaatc cccggtcatg gctgaccacg
    10561 atgaagcctt gttttttctt tttcaaataa gccgctactt gttttctacc agagatatcc
    10621 aaatgattcg ttggttcatc gattaacggg aaatgagtgt catccacaaa taaaagtgcc
    10681 agtaggactt ttgtcttctc tccccccgat agtgtgctga attctctcca taagatttca
    10741 ggatctgttt gcatcaattg gagctctctt tcgatttccc atatctcaaa atccgtaata
    10801 tcatttaaca cgtaataggt taaacgttct ttatcttttg tctgttgcgg gaaataggca
    10861 aattcttgct gatggattac ttgcccttga taaggtagtt tgttttgtag aatattcagt
    10921 aaagttgtct ttcctcgacc gtttcgtccg ataagtccta gtttccattg tgtgtcaaaa
    10981 tttagatttg cttgttcaaa taataatgtg ccttggctgt cgtagccgaa tgtcagattt
    11041 tttatttcga ttttagacat tttccatcac ctcgtaaaaa aagaccagtc ctattttcag
    11101 aactggctaa agaggatgca agggttggat acacacaaaa agacccatac atcgaaaaaa
    11161 ccaagccaat cccgaaaatc tgcacactgc ttccttgtct ccacaaggta cagtctgaat
    11221 gcagctttca tcaatcgact taatttttca atgcacggat ccctcacttt gtcctatatt
    11281 tgaaattacg atagcataaa gaaaatgatt ttgtcaacta cttcgggagg atggtgaaaa
    11341 atctgctaag tgtctgtcct aagatttttg ttttttctaa tgtttgcctg tgttccattg
    11401 cttcttctaa acctattgga cccaaatgaa tacttaagac aattgggaga aaatcagaaa
    11461 gtccttctag ctcttggtct ctacttccgc aaatagcgat tattggtttt cgatattttt
    11521 ttgcaagcac tgctacaccg tatggaactt ttccgtggat cgtttgctga tcgatccgcc
    11581 cttctcctgt aatgatgaca tctgcttgtt cgatcttttt ttccaatcca gtgatttggc
    11641 tgactaaatc gaaaccagaa accatcttcg ctcccaacaa aaaaagggct ccaccaatac
    11701 cgcctgctgc tccacttcct ggaatatcat ctaaatccac ctcccagaaa tcttttattt
    11761 gctgagcaat ctttcctgct tgtttatcta agaaagctag cgtcgtatct attcctcctt
    11821 tttgtcttcc aaattgccaa gcagctcctt gttgacctgt atatggattg gtaacatctg
    11881 cagcgataat caattttact ttagaaacaa gaacgtttct tcgatcaaaa tgaagttttt
    11941 tagtagataa taaaggattt ccttctgtgt acccttctaa agaagcaccc aaggattgga
    12001 gtaatcctag accaccatcg ctacacccgc ttcctcctaa agtgacaata atttgttcta
    12061 cttcttgaag cagtgcatct ttaataagtt ctcctaaggc ataactggaa gcaagtcgga
    12121 tagtttgatc cgttggttta tcaagtagat ctaaccccaa tgcttgtgca gattcgatga
    12181 ctgcggtgtt ttttccttgc caagttgtca ataaatagct gatttttcgc tgacggaata
    12241 agagatccat cccatcaatg gtacgccatg ttcccctgag cgcgtaatag attgcttctg
    12301 cagtattttc tccgccatct gcaatcggca cattgatttt tcttccttta cgatcttccc
    12361 agccatttag aactgcctga tttagttcaa atgaagaagc acttccttta aatgaatcaa
    12421 ttgccgccaa aatatccatc tgtatccctc actttcttct taagtaagac actttttttc
    12481 atttttttca aacaaaacaa tgctattttc ttccattttt ctatatacta aaagggaact
    12541 cattgaataa aggagagaaa aaacatgaaa atcgcaatag ctggggccgg agcaatgggc
    12601 agccgaatcg gattgatgct acatcaaagc ggaaatgagg tcctcttgat cgatcgttgg
    12661 cctgctcaca ttgaagccat tcgtacaaat gggttgattg ctgactttaa cggtaaagaa
    12721 gtggtcgcaa aactgccaat ctattcacca gaagaaatca tcgaaagcaa cgaacacgtg
    12781 gacttgatcg tagcattaac aaaagccaat caattagatg atatgttttg ttcgatccaa
    12841 tccattatta ctgacaatac ttatgttttg tgtttactaa atggattagg tcatgaagat
    12901 gtactggaaa aatatgtgcc aaagaaaaat atcttatttg ggatcacgat gtggacagct
    12961 ggtctagctg gtcccggaaa agtcacattg ttaggtgatg gagaaatcga actagagaac
    13021 ttggaaccag aaggcgaagc atttacaaaa aaagttgtag aagtctttca agaagcaaat
    13081 ttaaatccga tctatagcca taacgttcgt tattccattt ggcgaaaagc ttgtgtaaat
    13141 ggaacattga atggtttatg cacgattttg gattgtaata ttgctgaact aggcgcgcaa
    13201 aaagcggcag agagcatggt tcgaacaatc gtatctgaat ttgcttctat cgcagcaaaa
    13261 gaaggaatca tactagatca agaagaagtg taccaacata ttgcttctac ttatgatcct
    13321 gacaacatcg ggttgcatta cccatctatg taccaagacc tgatcaaaaa ccatcgtttg
    13381 actgaaatcg actacatcaa tggtgccatt tggcgaaaag gacaaaaata tgatattgct
    13441 acaccatatt gtgccttttt gacccaatta gtccatgcaa aagaaggaat ccttggcgca
    13501 gaataaaagg ctctctgatg agaatcagct gaatcagcag ctggttctct ttttttaagc
    13561 ccaataaaag atcaaaaaga aaacacaact ctcatgatgc cataagaatt gtgcttgttt
    13621 gtttcagtac ataatctctt caaaatgtag gttttttatt tgttttcaac agaggagagg
    13681 tcatattcgt attgctcata aatagtggaa tcgttagtga taaatcgttt tgcccaattt
    13741 tctgcatcat aaaattccaa tagctctgat aggattggct taaatggtgt atgcagaaat
    13801 tccggtaatt catctaaatc acgttctcga agcaaaaaaa tattttcagg acgataaaaa
    13861 tgataatgag caatcgcttg attatccgtc actagtttct tttgaaaata catggattca
    13921 aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca gttccagaat cccgcgcgat
    13981 tcccctgttt ttttcaggta ctcttcataa gtaagatatc caggttgttc attccctcga
    14041 tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta taaattcctg cttatagctt
    14101 tctgctcgtt cttttctccc attattcgtt gcaccaaaaa atagatccgt ctttggttcg
    14161 ctagaagaaa aattcatatg tttcgaataa aatgaactat tatgtttcag cccaagttct
    14221 tttgcttcca aaaaatcaaa atgataaatc tcatcgattg ccggttcatc ttgtgcctgc
    14281 ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa aaaataacac aatttttgca
    14341 ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa atccataatc taaaaaaacg
    14401 atcagctgat aatttgccaa ctgttgtcta gctgatttaa tgaattccga ctgtagtaaa
    14461 tcttttccgg accaaacatc taaagagcgg atggaaggca aaaaataaag tgatttattt
    14521 tttgcaacta gcagtacgtc atttttgttc atcttttccg cctcactttc tctgtcactt
    14581 tttcctatga tagcctgttt aaatagtaaa aagccagtaa aacgtttcct atatttgtat
    14641 gaaaatttaa catttgtatg aaaatatgat gaaataaatc attcttctat taaaattcgc
    14701 ctaaaagttt tctgaatagt tgtaattgtc tgaaaatttg ttatcattag tcattgttaa
    14761 atattgctat ttattcttaa tataaatttt gattgtgaat ttaaaagaaa gaggtcattt
    14821 atggaagata aaaaattgaa aaaacaactt agctccagac atatcaccat gttagcttta
    14881 ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg aagcgattgg gatcgccggc
    14941 ccttctgtat tgattgcttt tttgattggt gggctgattt tatatatcgt gatgaaaggt
    15001 ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg gattatctgg tttgatacgc
    15061 ccttatctag gtgctcattc tgctgatttt actgattggg tctactggtc tatgtggatt
    15121 atcaatatca tcgctgaagc tgttgcagct gcttcctttt tgcagttgtg gttcccgcac
    15181 attccagcct gggtatttgt ttttatgttg gctgttttaa ctacgatcat caatctttat
    15241 tcggtacggt tattcgcaga aacagaatat tggctggctt ttgctaaaat cagtgtcatt
    15301 atcttactga tcatttttgc tacttatctt gtcggtcagc aaatgttggg cacaggcgtt
    15361 ttccctaccc ttcagcaatt gactgaccat ggtgggttta ctcctcatgg tatgaaggga
    15421 atcatcagtt ctttgcttgt agttatctat tcttacggcg gttctgaact gattgctatc
    15481 acggtaagcg aagcagatga tcctaaacgt gcgattccta aagcaatcaa aggtgtcatg
    15541 ggaagaatca tttcctttta catcattcct ttgtttttgc ttttgattct ttttccttgg
    15601 aatactttag caggcacgaa tgtcagtcct tttgtgatgg tatttgaaaa aatgaatatc
    15661 ccatttgcag cggatatcgt caattttgtg attgtccttg ctttgttctc atctatcaat
    15721 tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt tgaaggatcg aaaaggttcc
    15781 tctagaaaac tagctgtttt aaacaagcat caagtaccgc aacgtgctgt tttattttgt
    15841 accagtgttc tttatcttgg tgtagctctt tcttattttg tcggtgacaa attatttggc
    15901 tatctggcag gttccctttc gtatacagta ttattgatct ggatcattat cagtgcggca
    15961 tcctttgtac tggcactaag aaaaggaaca ttatgggata aaggaatcag cttttttgca
    16021 ttagctgtct taggattgat tttccttggt atcctgttca cgaactccat tggggtaact
    16081 gtattgactg gcctgcttta cctgttcatt tatttcagtt atcaaaaaaa gaatgatgcg
    16141 ttcgtattga cgaatgaatg aaatcgatag aaaattttat tttatttatg tggaatagat
    16201 ggagctattg aagaaaggag ttgtgacaaa agtcttgttg caactccttt ttccgaataa
    16261 acagtctcat gacctcttgg taaacggtgt tcaaaaaccc tctgtgcaaa ataattgatt
    16321 gttctccatg ctcatgacat gatagaagaa aggaatgaaa aaaaggagtt tgttttaaat
    16381 gaaaaagtat tggaagtttt tgttgatgat cggtgtttca acggtagtta tgtttggtat
    16441 gatgtacttg aatgtctatg cgctcgatca tatcttcttc agtcgtacac gagtattcat
    16501 ggcgttgatg atgggtgcag tcatggcagt catcatgctg ttgttcatgt ggaaaatgta
    16561 tgaaaacaaa aagataaatg ttatgatctt agccgctagt cttcttgttt ttgccggttc
    16621 cttgtttatg gtacgaagcc aaactactgt caacgacaca gcttggatga aggccatgat
    16681 tcctcatcat tctatcgcta tcctggcaag taaacgagct gatttaaaag atcctgaagt
    16741 aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa gagatcgaca aaatgaaaaa
    16801 aatgatcaaa gaaatggaat gaccattatc cgtccaaata aaaaaagagg atgttccaga
    16861 agttatcagc ttcaagaaat aagaagaagc tacttctgga acgccgtgaa taaggggttg
    16921 tgaaaaagct gccctgcatc aagtaataag aacagccaaa caaaaatagc ttctcctatt
    16981 tttcgtttgg ttaggcttat taccgcagat gcaagctttt gaacaccgtt tattaagagg
    17041 ttgtaaaatc agcgttctga cctgagtatt agagagacaa atggaaaaat agttcctcat
    17101 attttgccat tttttgagct aataccgagg gtcagctgat tgaacaccgt ttatcgagag
    17161 gttgtgaaaa agtcgcttag tttcgcgtat taagaaaaat tttgtgaaat ggctttccac
    17221 atttttgtat aaatttggct taataccgag aaactggctt ttgaataccg tttattcgta
    17281 aaagggattg tgacaaaagt cttgtcacaa tcccttttta tgtttttatt gttctaattg
    17341 cgctgtcttg cttactgctt cttgcggttt cacaaatttt tttgagattc cccaacctgt
    17401 gaaggcactg accatcgaaa acacaggaga aagcaaactg aaaaatacga atggcaagta
    17461 agtgagtgta ggaataccaa atgtacttgc tacgaaactt ccggcaattc cccaaggcac
    17521 taagtagttg attactgttc caccatcttc tagcgttcta cctaatacag ttggatcaaa
    17581 tcctgctttt tcataagttt ctttaaatgc attaccaggc agaataacag ataggaattg
    17641 ttcaccaata aaaatattta ctccgatact tgtcatcaat gttgtaaaaa tcaaatttgg
    17701 tgtgttcgtc atctttcttg atactcgttc catcacggtt gagatcaagc caaattcgat
    17761 caacaatcca cctaatgaca aagttagaat aatcaatgac acagtaggca tcatactttc
    17821 gattcctccg cggctaagca gtgaatcgat ttgttggttt ccagtatttg aaacaaaccc
    17881 tgattcgatg accccagcca acttcgttaa ggaagtttgt gggttttgaa taaaaatcat
    17941 tcctactgct acagcgatat tcaacaaaat cgttaaaatt gccggcacct ttttccatgc
    18001 acacagtaac attaaaaata aaggaatcag tgaccagatt gaaatagtaa agtttgcttc
    18061 tagaacatca gtcacttcac gaatagatga aagacttgcg gctttacttg tgtgtcctaa
    18121 aatcatgaat aaaaagaaag aaacaacaaa tgccgggaca gttgaccaca tcatatgctt
    18181 gatgtgtttg aacaaatcgg catcaacaat agctgctgct agatttgttg attcggataa
    18241 tggagacatc ttatctccaa aaacggcacc tgaaacaata gctccaacaa ccagcgctgg
    18301 atttaagcct agtgttgtcc caataccgaa aaatgcaatc ccaatcgttg acataacagt
    18361 aaaggcactc cctacagcgc tacctacgat tgcacacaca acaaatacag aagggacaaa
    18421 ccactggaca ctgatcaatt tgaacccaat gaccattaga gttggaatga tccctgcttg
    18481 tatccataca gcaatcaatg aaccgactaa aataaaaata aagattggga tgattcctgg
    18541 tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc gggtatcttt ttatcactgc
    18601 atacaacatg accattccga tcgctgctaa gatcgtgata ttaggtgaga cctttaatcc
    18661 gacaacgcca atcgcaatgc ctaaaacaat aaccattaat acaagtgttg cttctaaaaa
    18721 acttggtgtt cctttttttc ccatgataat ctctcctttt ttagttgttc ttatgttcta
    18781 aaaaagttaa gatatcggat tactccgccg caattcatcc gcgattcctc ctttataaaa
    18841 ataaaaagac ctgttttaaa ctaaggacga atgactccgc ggtaccacct taattaaaaa
    18901 cagatctgtt tttcactttc aaacttttat tcaaatgtat ggactttcca tgtaattcgt
    18961 caattaggct tgatcggttc acagcgacca ccgactttct gaacaaaaca cctaaccgct
    19021 actgcgtgaa aagttgactt gtacgaagtt taccttccat aagatcattt gtcaatgaaa
    19081 aagaaaatat tttttcattt ttctgaaaat tatttttata actttcacac acaagattat
    19141 tttttactag aaagtagcgc cctcttgttt ttttcttgct actatttctt gatgttcttg
    19201 cttgattctt tctaatagct ttggctcttt caacagatca agagctgtat tggcgagtag
    19261 ttctgctgca atcgcaatag aagcaaggcc tttgtcactt ttagcagcag ctttgaattc
    19321 ttctgtgtgt ccggcaatat atgtctcaga gattgctacc gtaggctgga ttgttggtat
    19381 gacttggctg acatttccta catcagaaga accgagactt gttctttgtt ccgtttctat
    19441 ttcttcatct ggaacacctg cttcttttgc atgctggaag aataagtcat caaaagaagg
    19501 agtaacgatg acatcatcaa ctgcattttg gaacaatcca aactcaaaag tcgtgcccgt
    19561 acttatcgca gcacctcgta cgatattttc cactttttca tatactttat ctaatgtttg
    19621 ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa tctggaatga cgttggcagc
    19681 tactcctccg tctgtaatga tcccatggat attgacatct tttggcaatt gcaagcgtag
    19741 agcgttgatt ccattaaaca cttggatcaa tgcttccaaa gcatttactc ctttttcagg
    19801 tgctgctgct gcatgggaag cgacaccgta aaatttgatg tcgactggat cattcgctaa
    19861 agattctgtc gttttcccat aacgaaaagc tggatggacg cataacgcta catcgacatc
    19921 ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat ccgttctcgc ccccttcttc
    19981 ccctggggtg ccatatacac ggatttctcc accagtttca tcaatgaatt gcttcactat
    20041 gctggcggct aatacggagt aagtaccaaa caaattatgc ccgcatgcat gaccaatacc
    20101 tggtaaagca tcgaattctg ccaaaaaggc aattgtggga ccgggtttat tggaaatata
    20161 acgtgcatca aaggctgttc ggtgtccagc tacttctttt tctacccaaa acctttcttt
    20221 ttctaattgt ttaatcaacg ctgctgagga ataaaactca tagttactta cttcaggatg
    20281 ttcgtggata tctagagcaa gtgcttgata ttctggtaaa cgatattgga ttagatcctt
    20341 caattggttt gttgagattg ttgttgtcat aaaaaattcc tcctatgcta gtcatttttc
    20401 ttattttctc ccgaatgttt cccatgcagg cacgcttgat ccttttgatg ttttctcgat
    20461 tactttgact gtgtcttctg tttgataagc gtctactatt ttttgatacg ttttattgtt
    20521 ttcatcttct tttcttgcaa cgatgatatt cacataagga cgtgcatggt catccactgg
    20581 ttctaagaag atggcatcag aagcaggatt aaatccagca tctacagcta caccgctatt
    20641 gatcactgaa atgtctacat ctgacaaagc acgtgctgtt tgggaagcat ctaattcaga
    20701 aatgtctaga ttttttctat tctcggtaat atcagaaaca gtgggcgctt gtccttttgc
    20761 cttatccact ttgatcaatc cagcagtttg caataacaga agagctctgc cgccatttgt
    20821 aacatcatta gggattgcta cttttgctcc gtctttgata tcagctacat ccttcaaagt
    20881 gttagaataa attcccagcg gtgcattgac agtatttccg atcgatacta aatcagttcc
    20941 gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg aaagaattca aatcaatcga
    21001 tccatctgct aaagccacat ttggttgatt gtaatctgta aatttcacgt attccaaatt
    21061 aatgttctcc tttttcaaac ggtctttcac gttgtcccat acatctgtat cttctccaat
    21121 aatccctagt tttacgatag tttctttttt gtcagttgta tctgcttttc cttggctaca
    21181 ccctgccaac caaataattg ctacaaatgt aatgactaca aaacgaataa tttttttcat
    21241 tttttctcct ccactttcaa tgtgtaattt ttttaatcag ttgattactg ataaattgac
    21301 ttaaaaaaac taagattaga atcaataatg tagccacaat cgttacatct gtttggaatc
    21361 gattataccc tctcgatata gcaagattcc ctaatccgcc tgcacctact gctccagcca
    21421 tggtagtcag tccgatcacg ttgatgatag tcaacgccga gacccgaatg ataccaggca
    21481 aaccttctaa caaatacaca cgtaaaatga taccgattgg actggttccc atcgcttctg
    21541 ccgcttcgat caccccagga tcaacttcta gcagtgcgtt ctcgatttga cgagcaaaaa
    21601 acggaacaac tccaaaaatc aatggcacta aagctgcttt cgcaccaatc gtcgttccaa
    21661 ctaagaaacg agtagtcaat gctaaaagag aaagcaagat aatgaaaggg atggaacgaa
    21721 tgatattgat cagcttatca agtatttcaa atagcagacg gttagctagt aatccattag
    21781 ggcgtgtcac tactaacgtc acacctaaga tgattccaat catcccgcca atgaatgctg
    21841 tccaaaaaga catatatagt gtttgcttcg tcgcttcgac aaattcatcc ggaatcaaat
    21901 aaacattggg caaatatgtt ttaaagaaat cagtcatttt atcttcctcc ttcgatcact
    21961 ttgatacgac tatctaaatc gtttttttgt aaaatattta tttttacacc agatttgatc
    22021 aaataatcca atgctttttt ccgctgtttg gtttctccag ctaaagtaac gatcaaactc
    22081 cctaacggcg tttcttgtaa taactctaca ttgccatata aaatattcgc tgttacttga
    22141 tatttgctgt acagatgagc gattagaggt tcgttcgtct gatttccaat ataacttaat
    22201 tccaccagcc atttattggc gatctgatcg gcaaaagcag aatgggcaat gatcgtttct
    22261 aatgcttggt caagatgagt agctgttcga ataaaatctt tggtcaattc ttcttcagga
    22321 ttgctgaaga tctggatgct gctacctttt tcaacgattc ttccatcttc cataaccgct
    22381 accttgttgc agatttcttt gacaacttgc atctcatgag tgatcaggac gattgttaaa
    22441 cctaactggc gattcaattt ttttaacaat gcaaggatct gaagggtcgt tttcggatca
    22501 agtgcactgg ttgcttcatc acataataat accttaggtt cattcgccaa agctcgtgcg
    22561 attgctactc gttgtttttg tccccccgac agttgtttag gataagcaga tgctttttct
    22621 tccaaaccga caagttccag tagttcattc actttttgac gacgttcctg tttcgatttt
    22681 ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag ttcggctttc cattagattg
    22741 aaatgctgaa agatcatacc gatggccttt ctttcttttc gcaattcttt gggagagagc
    22801 tgactcagat tcgtctgatt gacaatgact tctcctgctg ttggtttctg cagcaaattc
    22861 atcacacgaa ctaacgtact tttccctgca cctgagtaac cgacgattcc ataaacatcg
    22921 cctttttcaa tcgttaaatc tacagaatct actgcctgta tctctttttc tttttgtttg
    22981 aaacatacag aaacatctcg caattcaatc attaaaattc ctccttatct aaaaaaagcg
    23041 tcctcaagaa aatgaaaatg tcattttctt gaggacgctt ttttgcgtgt taccacctca
    23101 tgttcaagct gtgttcacac acaactcctc ttcaagtacg atcatactct agcaagatat
    23161 cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg aattgctcga tggccatttt
    23221 ccaatagtcg ttccttgctt cttctcagct accgaagctc tctgtcaaag gttcttctat
    23281 cgtacttacc atttcaatgc aaataatatt caaaaaaaaa gtccttttac taacataaac
    23341 tatgtcagta aaaggacgac gtatcgcggt accacctttt ttcggaagtt tccttccctc
    23401 aagcccaaca tctcaaaaag agagattggc gttcgtttta acggtcgaac agttccgaat
    23461 ttgcctactt atcgacaaat tattctccaa ggccattttc agcttttctt acttgactct
    23521 tctcaccagc cagagcctct ctgcaaagtt tccaaactta ctttcctttt caacgaaatc
    23581 gatattttgt tgttgggatt aatctacatg aggaaaactg aaaagtcaag tacgaaattc
    23641 aaattatttt ttctatattt atttttctgt ttttttattt aacatttcaa taacagttcg
    23701 aagatctttt ccttccgcaa gaagtttcaa tttttccttt tcttcgacgg tataatttcg
    23761 gacaaaactg gaatcaggta tcaaaacaac gcctaatcct gctcgtgaag cagctgttgc
    23821 tcctactaaa gaatcttcca ctgcaaaggc tgaactagct tctaatcctg ttcgttccaa
    23881 agctgtcaga taaggaatcg gtgaaggctt gttctccgac acttcgtctc cgaatgtaaa
    23941 cgtgtccaag taatcagcta atttaaagtg atccaagata tctgttgctc ttttcgtaac
    24001 agtggtcgta gcaatgccta gtttcatatg acgttctttt aagtctgcca atatttcttt
    24061 tgcatatggt tttaagtgga tggctccgct tgtcagctgt tgataaaaat attcttcacg
    24121 cttcgctcgg acttcctgta ctttttcagg gctacctgct gctttcacta aaatatcaaa
    24181 actttgtttc cagttttttc cgccccaagc gtcaacgatt tcttttggga tatggatatc
    24241 aaattgctga aatccccaaa gccagccatc acgatacgtt ttttctgtgt caactaacaa
    24301 accatctaaa tcaaatattg cacctttcat ctatacatta atcctttcca aaacaataca
    24361 catagtttag cacgactggt ccaaaaagaa cagaagcaaa gaaccagcct tctcgtttat
    24421 aagagttctt ggaatcgacg gatatagatt ttcttcacag ctgtcacaag cgccagataa
    24481 gcgatgactg tagcaaataa ccataaccag taattgccag gaagtggcat caagcctaat
    24541 tcagcaccaa aacttgtaaa aggaaggaca gaaccgacaa taattcctaa tgtagtgata
    24601 acaaacatgg caaatgttgc gttgctttgg ataaatggta ctttcggtgt acgcagtgca
    24661 tggagcacta aggtttgtgt ccatagtgat tcgacaaacc atcctgcatg gaataatgcg
    24721 atgaaaatca ctcgctgtga actatccaat gtatggaaac ttccgcccac tacagcagga
    24781 caaataacaa aatacatcaa caagtaagta gtaatatcaa atatcgaact tgttggacca
    24841 aagtaaacca tgaattcccc aatactagat gcattccatt tttttggctc gtccaaatat
    24901 tctttatcca tccgatccca tggcaaggaa atgcaggaaa catcatagat cagattcaaa
    24961 aacaaaagtt gtaaaggcag cattggtaaa aatggcagga acgtacttgc aatcagcacg
    25021 gaaaacatat tgccaaaatt agaacttgct gtagccttga tatatttcat gatattccca
    25081 aaagtttctc gtccagaaag aaggcctctt tccaaaatcg tcaaatcttt ttctaaaagg
    25141 atcacgtctg cagattcttt cgcaatatcc acagctgtat ctacagaaat accgatatca
    25201 gccactttca ttgcaggagc gtcattgata ccatctccta aaaatccgac agtatgacca
    25261 tttttacgta aagctgtcgt caaacgtgct ttttgctgag gattcaattt tacgaatact
    25321 tcatggtctt cagctacttg tgctaattca tgatcactca tttccgagat tttctcaccg
    25381 gtaaccaatt cattgatctc tagacctact tgacggcaca ctgaacgtgt aactaattcg
    25441 ttgtccccag tcagaacttt tacagctaca ccatgttctt tcagagcttt caacgcttgt
    25501 ttcgttgttt cttttggtgg atcaaggaaa gcaagataac caatcaaaac catgtctgac
    25561 tcatctttga ctgaaaattc tccaacgacg cttggattgg tcttttgtgc gacaccaatc
    25621 actcgtaagc catcttcatt aagtgactgt acttttgcaa gtacggcttc ttttcgttct
    25681 tttgttaatg gtgtaacagt accgccaaga tcaataaatt tagaaatgtt cagcatttct
    25741 tcaatcgcac cttttgtgat catttgtgtt ttaccagctg gatcagcaac cacaacactc
    25801 atacgacgac gttcaaaatc aaaaggaatt tcatccactt tttgataatt gatcttatct
    25861 gtatctaaag tttctgttgc ttcatcaatg atagccacat ctaacaaatt tttcagccct
    25921 gtttgatagt agctatttag atacgcatgg cgcaaaacac gatcatcttc atttccttca
    25981 caatccatat gatattcaag aatgatttta tcttgtgtca atgtccccgt tttatccgta
    26041 cataacacat cgattgcacc aaagttctgg atggcattca aatttttgat cactgttcct
    26101 tttttggcca tcacacttgc tcctttgacc aaattcgtcg taacgatcat tggaagcatt
    26161 tcaggtgtta gaccaactgc tacagatagt ccaaaaagaa aagcttctag ccaatcccct
    26221 tttgtaagcc cattgatgac gataactgct ggagccatca atgacataaa tttgatcagt
    26281 agcatggatg ttttatggat gcctaattca aagcttgatt gaattggttt ttcagatagc
    26341 gtttctgcta catgaccaaa tagtgtttca tttccaacag aaacaatgat tccttccgca
    26401 cttccactga tgacattact tcccataaag acaagatttt cgtaacttgt ttcgctttgc
    26461 tgatcgtctt cacatacttt cgcatgcttt tctactggat agctttcccc tgtcaatgct
    26521 gcttgggaaa tgaataaatc ctttgcggac aataaacgca tatccgctgg tatcatatcc
    26581 cctgctgaaa gccgaacaag atctccacag acgatttctt ccataggaat ctctgtgtac
    26641 tttccattac gttttacagc tgccgttact ttcacaagtg atttcagttt ttcagccgct
    26701 tgattggaac gtacggactg aatcaacgtc attgtcccgc tgacgataac catagtgaag
    26761 ataatggcta caccgaaaag atctttatct ccaggagctg ctaatacata ttccgtgatg
    26821 aatgaaataa ttcctaatgc gatcaacact aatgtaaatg gcgtgatgta tgctttcaat
    26881 atttctacaa tgaatggtgt ttttttgcca tatgaaatcg cattttcacc gtaattttct
    26941 cttgaacgtt cgatttgttc ttctgtcaat ccatttgctg atgcatggaa tagttcaaat
    27001 aattcttgtt tcgtttttcc agcaaaagct gcatagattt tttgtgtatc tttctttttt
    27061 tccatctcaa tctctccttt gtgtccaaaa gacagctgtt ctcgctgctt ttggtcttta
    27121 gctattcatc ggatcgatag agaaaaaact gaaaggggct gagaacagat agataatagc
    27181 cctggccttt cctctagatc cttgtttttc gaatgactgt catccatttg ttctcatctc
    27241 ctttactaag taattttaat tcatgacttc caatcatttt tcgaatagtt atttttccaa
    27301 ttagaccacc tcctttttat ttttcaccga tggggatttg cggcaacaaa aaaacctctc
    27361 gccaaatact cagcgaaagg tttgaaaaag catattaaaa aaagcttctc agatctcatc
    27421 gttgagtttt gacactgtac aacgtaaaaa gcagatacct gcttttagct accttatgaa
    27481 aagccttatc tcgacaattc ctgttctacc cattggcgtc tctcgacgtt tttgggcagt
    27541 agcctatgtt tgtataggag cctcacctaa caaatcttat tcggttttcc ttcgttaata
    27601 taacaactaa aaaagaggat gtcaaatcaa ttttccatgt ttttcatact ttttttaaaa
    27661 tacacacgtt atattcctgt ctcacgcggt tttatttgtc tcattttcca cctcccgcta
    27721 taatagaaat atagtaggaa acgaggtgct gttatggaaa gaacacttgt tattatcaag
    27781 ccagacggcg tgagacgcca tttagtcgga agcatcatcc aacgttttga agcaaaggga
    27841 ctggccattg ctgaaatgaa gtttgaaact atgactcctg aattagcaaa agaacattat
    27901 cagcacttga ctgagcgcag tttttttgat gaactgatcg attacatgac ttccggtcct
    27961 gtcgtttatc ttgtgctggt cggcgaggaa gtgatcgata tcgtccgtaa gatggttgga
    28021 gcaacaaaag ctgccgatgc tgttccaggt acgatccgag gagactacgc gctgccgggg
    28081 actgaaaaca tcattcacgc ttctgattcc cgtgatgctg cggtaaaaga gatcgcccgc
    28141 tttttcccgc cattcgatat aaacaaaccg tctgaaaaag cggcgaagtg atacaaattc
    28201 taaactgctg gtctagttgc tgggttcagc aatggccagc agttttttct ctaaggacct
    28261 ccgttgattc tttcctaaca tatattcgag cactgtgaag aattcttggt agtctttcgt
    28321 cttttcttcc cctaaagcag cagaaatcaa tctttgataa taatctgcca tcaatgtttc
    28381 atagatcact gtacttcttt ctttatgtgc taagctccgt ttcatatctt cattcacact
    28441 acgtgccttt ttcaaccgac cattttctat ataaaacgaa gtcaggttca ttaaaaaagt
    28501 aaagagatac tgctcatgat tgttcaactg tgctaatcgt cgcattcttt ttttgatttt
    28561 caaataattg ttctcaatga accgatcact gaatatccaa ggaaaatcag ctagaataga
    28621 tagttcaaat tgaccatagt ctggtatctg catcaagtat tcttcaatga ttcggcattc
    28681 ataacgtaaa aattcaaatt ctgtctcaga tacttctgtt ttttcgacca tgtgaagatt
    28741 caaaaccaaa taatacagat cttctgtttt cttaaaatgc ctacggttat tgctgatctc
    28801 tttataagga tcaaagctgt cttcttgtac ccgatctagc attcgtcttt ttttctttaa
    28861 ctttattggt tcatccagct ggtttcgcat ccattcatat tctgcaagtg tcatgttcat
    28921 tcgctctaaa aatgatctca atgttacaaa tgacattttt ttcactgaat ttgtctcaat
    28981 cccgactaga gaagtcttaa aacagattcc ttccgctaat tgggactgtg tcaacttctt
    29041 gttgactcga agtttcttga tcaattcacc atcatacata atagttctcc tttttaattt
    29101 aaatttttcc acattcaata aatagatacg ttaaaaagga aaaatacacg aaaaaaataa
    29161 aaaaatgttt ttttcataaa aaataccaac cttcaagcat tagaatgttg tctaactact
    29221 gaaggttggt ttgattgttt attgattatt tttttgctgt tcaacgtctg ttcttgttac
    29281 tggatataag ttggttgttg ttttattacc attttttgaa aagacacttg tcgaatgttc
    29341 gcctttttgc ttttcgattg cttcattacg ttccgcttca tttttatagt catatttttt
    29401 cggatcaatc gtctcaaatc cgtttggcac ataaaaccgc aagagatttt cctgattcag
    29461 cttatctgaa agacttaacg cagtcttgac tttttcctga tctttggaga tttcctcatc
    29521 gattcctgct gctttcgtat cgatgatttc tcctgtctga ttattgtaag cttttcctcc
    29581 taggacggtg tatttgggtg tcacaaagtt cccatttcga aaagcaacta cttgatcatg
    29641 ctgagaagat aataagtctg taccaaaatg aagataatct ttatcatcga ttcccaatag
    29701 atgtagtaat gtcggcaaaa catcgatttc tccaccgtat tcatgattga ctgtcccttt
    29761 agtataacca ggcatatgga tcatcaatgg cactcgttgc atctgtgcgt tgtcaaattc
    29821 atcccatgta tctggatctt taccaagagc gcttgctaaa tctttattat ctgtattcga
    29881 gatcccaaaa tgatccccgt aaatcacaaa gattgaattt tggtaaatac cagatttttt
    29941 caaatacgta aaaaattgtt ccaatgattg atccagataa tgtgccgttc gaacataatt
    30001 atctactgtg ctattgccag tattcaaaga tggaaagtcg aaattcttgt catccgtata
    30061 ataaggcgtg tggttggtta cggaaagaaa tttcgtgtaa aacggctgtt gtagatgctc
    30121 aagatattgg gcagattctc tgaataaatc tttatctaat atcccataac ctaacgtttc
    30181 gtcggattca tcaaaataag atcgatcaaa gaaattgtca taacctaagt ttttatatac
    30241 atgatctcta ttccaaaaac tagctacatt cccatggaaa acagcgcttg tatagccttg
    30301 cttttgtcca aggatcgctg gtgcggcttg aaagacattg tctgacccaa gttctgtgaa
    30361 taatgagcct tgaggtaaac caaatgtgcc agtttccaac atattttcag catcacttgt
    30421 ctttccctgt cctacttcat gaaagaaatt gtcaaagctg atcgttgctt gattctggaa
    30481 aatactattt aaaaacggtg tgacttcttg tccgtctacc ttcatattaa tcaaaaactg
    30541 ctggaaactt tctaaatgga gaacgatcac atttttccct ttagcgatcc caaatgtttc
    30601 gggatttggc tctgcataat gtttctttgt ataatctaaa acttcatcaa tcccattact
    30661 gcttgcatgc gcgcgaacac tgctagtcat tcctgttttg attccgtcat aaatagtata
    30721 cgcatcgatt cctagatatt tgacaatata tgaacggtca aatgttcgct gcagcaattg
    30781 tggacggttg gcttcgctca gcgctagatt tcctgaaaaa ataagccctg aaagacaggt
    30841 aacagcgata gccatcggtt tgccgacagg gttgcttttg ataggtattt tcttgacctt
    30901 caaccaaacc agtagcccaa tgaacacagc gatatccagc cagtagatga catcatgggg
    30961 cttcatcaaa gaaaaggagc ttcctgacaa tccttgcgac actttcgaaa agcctaatac
    31021 agatttgact gtaatgaaat ctgtgaattc ccgataaaaa ataatattca agtacaacaa
    31081 aagcgtattt aaaatatcaa tgatcaaaag aatagctaac gctggctttg gctttttaat
    31141 atacaaagaa aggccaaaaa acaataaggt agtcgcaatt gggttgatcc acaagataaa
    31201 gtactggatc gtaccttcca cacctaatga aaaatctgca tagtaggcaa tgatcgtctt
    31261 cagccagaaa aagaaaacag ctgtcaaaac gattgctaaa cgattttggc acaatttttt
    31321 aagttgtgtc acgtatatca cttctctcta ttcacatttt ttcttatttt gctcctgaaa
    31381 aatgaaaatt tcctaaaaaa gttgttatct taagattttt ttattctttc taaagattat
    31441 tccgtccact tttcacagca taaaaccgct gcgatgtaag cgattaaact aatgaagaga
    31501 taaaagtctt gtggtgaccg cttcactcca cggtattcaa aagccagttt ctcggtattc
    31561 gtacaaaaaa agaggctgtg acaaaattct tgtcgcagct ccttatttct tgatgcagga
    31621 cagctttttc acaacttctt ccttaaacgg tgttctcctg ccaactcctc ggccacaagt
    31681 cacaatattt ataaaacatg agaagctgtt ttttgaaatt gttccttgtg gctcagagtt
    31741 aaacggcagg ctcaagacct cttgatacac ggtgttcaat cagctgaccc tcggtattag
    31801 attatgtcag gacgctaatt ttacaacctc ttggtaaacg gtgttcaaaa gcttgcatct
    31861 gcggtaataa gcccaaccaa acgaaaaata taaggagcta ttttttgaaa ttgctccttg
    31921 tggctcagag ttaaacggca ggctcaagac ctcttgatac acggtgttca atcagctgac
    31981 cttcggtatt agctcaaaaa acggcaaaat atgaggaact attttgccgt ttgtttctct
    32041 aatactcagg tcaaaacgct gatttcacaa cctctgtttt tcattgtcca taggattgat
    32101 attgtccttg tgatgactca ttcgtaataa ttgaggcttg attttcttca cttcccgtta
    32161 tcgtttgtcc actgccagaa tctgaaggac ttgttcctgt cgactcgttt ttttgagaag
    32221 agacagttgt tttcttgact tgttcatttg tctttttcca ttccgctaaa cgatcggcga
    32281 ccactttttt gtctactttt tcagaataag cggtgatcac ttttccattt ttcaaacggg
    32341 ctactttgtc gccatcatga gtcaaaagca aaacggtcaa atgcttgtct ttaaattgat
    32401 attgtctgag agaaatcgca ctaagtacgt catcgatatt aataaattga ttttcttctt
    32461 ttccttttgg caaataaatg tccaattcgc ctttttcgta attggcagtt attttatact
    32521 ccgcccacca ataagttgag aattcttttt tgaaattatc tactgattct ttgcttgcta
    32581 cgattttttc tgtttctttc tgagcaagat ctgctgtcat ttttcctgaa aatgctgcgg
    32641 ataaaataag cagtaagaaa aaaccgaata accaaaagag aagaaacggt ctttttttcc
    32701 tcatgctttc ctccactcct atttaatttt gactattcta ttatatacaa aaaatgaaat
    32761 agaaagacaa aaaagttgaa aaagatcgta aataattgaa cttctttttc acaaaatggt
    32821 aagaatggag aatttcacgc atttgctctc aactattctt tcttaagcct caaatccgtt
    32881 tttatctgat acgtctttga accaatgacc cgattttttg attgttcttt ttccatcttg
    32941 tgctaagtct actgaaataa agccatatcg attcttatat gcattggtcc acgaccagtt
    33001 atccatacat gtccacatat gatagcctaa acaattggca ccttcttgaa tggcttgatg
    33061 gacatatttc agatgagatt ggacaaattc gatccgataa tcatcgtgga tcatgccatc
    33121 ttctttgata aatcgtgctt cgttttctac acccataccg ttttcagaaa taaagcagtc
    33181 gatatttcca taatgatctc gtacattgat caaaatatca tagattcctt gttcatagat
    33241 ctcccatccg cgataaggat tcattttacg accaggcatt tcataattgt cgaaataatc
    33301 ctcaggcatt ggaccgtttt tagtatcgat tggtgtttct tttgctttta cccggcgtgg
    33361 ctggtaataa ttgataccta aaagatctac tgtattttct ttgataaccg tcaaatcctc
    33421 tgcatggatc acaggcatga gatcaagttc ttttactatc tcaatcaatt cacttggaaa
    33481 ctcgcctttt acagctggat caaggaatga acgattgaag aacgcatctg ctatttgcgc
    33541 tgctttaaca tcttcagggt ttgattcgtc tcttggatag cttggtgtca gattcaagat
    33601 gataccaata acaccacctt gctccatttc atggtaagat ttgatggcta acgctgaagc
    33661 caacgcttca tgatagccaa cttggatcgc ttttttcata tctgactctt ctggataatg
    33721 ccatcgatat aaataacctg cttcgactgg aacgattggt tcattatgag taaaccattt
    33781 ttttactttc gaaccaaaca attcaaaaca acttttggca tatttttcat aggcttcaac
    33841 cgtttctcgg ttcaaccatc cgcctttttc ttgcagatac atcggcatat cgaaatgata
    33901 cagattcaca aagggttcga taccgttttc aatcaactca tcaaaataac gatgatagaa
    33961 acgaacggct tcttcgttga ttgttccaga ttcatcaggt atcaaacggc tccattggat
    34021 agatgttcga aatgaattat gcccagtctg tttcatcaac tgtacatcct cttgatactt
    34081 cgtataaact tgcgaggttt tttcaggtcc tacttgatca aagaactttt gcggttcttt
    34141 tttgtaccaa taatcaaaaa tgttctctcc ttttccatcg cctgaaatcc ttccttctgt
    34201 ttgtgggcca cttgctgcag aaccccacca aaaatttgct ggaaattgat attttttcat
    34261 caaagcacct ctttttcttt tttagttcgt tttagttgat tccatgtatg atattttcca
    34321 tcacaatcac tttttcattt tcttcctctt cttttttgat tcgttttaac agctgttctg
    34381 ctgctcgttc cccgatttta tctgttggtg gcgtgatcgt gattttcgga aggatactca
    34441 gatcgttttt atctaaataa tcatagtgac tgaacgcaat atccttcccg attctgacac
    34501 cttcttgatt gaaacattcg atggcaccaa tcgacatatt gtaattgctg acaaaaatag
    34561 cagttgcccc tttttctaat aatgcttttg ccccgtaata gccgcttttt ctagaatagt
    34621 ctcctgtaaa gacctctaat tggatcgatg gatcattttg gaccgcttct ttggctcctg
    34681 ctaaacgttc tcttgctacg tagtctgttt gagtggcagc aataaatccg atttttttat
    34741 gtccttgttc accatgatga gcgatcactt ttgctacact ttctcgatca ttcactaata
    34801 ttgtatcaat acctgctggt ccttctggac aattcaatgc gataactgga atactcattt
    34861 ttgtgagcat ttcaaatcca gaccactctt cttcggataa gaagacaatc agcccatcga
    34921 tcacatgctt catcaggtat tcaatttttt gttcaatttt ttctggctga tcggcaaacc
    34981 cgcttaataa taagctatac ccattttctt cagctactct ttcgatccct gagacaactt
    35041 ccgacccaaa acggctggaa atcccgttta tcaaaagtcc aatagagaag cttcgattat
    35101 tcttcaaccc tttagcaatg atgttttctt tatagtccaa cgctgaaatc gcttctgcta
    35161 tttttttcat gttcttttcc ttcagttgct gaccattcaa gtaacgggaa actgttccca
    35221 ctgaaacacc agccatctta gccacatcac ggatcgatgc catacattct cactcctgcc
    35281 agttacattt gatttctctt tcttgttgtt cgattatgat gaccgcattc tttgcatagt
    35341 gttgcttgtc atcaatatcc atataactaa agtcttcagt agagcaactt cctctcacta
    35401 ttaattatac ttaaacaagt atttgaaccg gttcattttt aatatagcat ttttttaaaa
    35461 gattgcaaca cttttttaaa acgctttctg aaagtggcgg attttttgcc tttttaaaat
    35521 tttattctaa aatttactct atttgtgggt gttttgtgcg tttttctctt ccatccgttt
    35581 gaagttttac attccaatgg tataatttct ttatcctatc aaatggggga agtattttga
    35641 agaaattaat cgtactattt attttgtctg cgggactttt gacattgact ggctgtgatt
    35701 tgtttggcgg ctctgaccaa tcctcggacg aaacaaccga ttcttctgtc ttaaaagcgg
    35761 aaaaagcaga taccaccaac agtgtatata cgctaaaagt acgtaaagat atggatttat
    35821 ccgcgggaca gttgacattt acagataaaa aaatggaatg gacacgcacg tatcagacac
    35881 aaggaactga ttcttctgaa tcatctgaag aaaaaacagt gctgacagat atcaagatca
    35941 ctaccaaaga tgacgaatat attatttcag gaaaagaaaa taaaaaagaa acaacagtca
    36001 tatttacaaa aatcggtaat taccgtttga aggatgagga tggcaacatc tacagtctat
    36061 agtgccgtct gttggatctt cttttgtttc tccccttctt ttctgctata gtaaagaagc
    36121 aagatgattg taaaggagac gaaatggatg atcgatagtc ctgattattg tacatgtaat
    36181 aacaacaaaa atatccatac agaatttaat gaccaaggaa gcttgagcat atgcgacaac
    36241 tgtcacaaac tagtcaaaga ttcatttaaa acatttgaat cttatcctgt tcaaaattat
    36301 gtaaaaagta gaagaagata aacaaatcca agcaaaactc actatggctg atttttatga
    36361 acttcaaact cagcttcttc ctttgatcaa gcttcatcag taaaacaaac agtggatcat
    36421 ttcagtttac aaatcaaaga gtaaagattt atttatttca aatatttgaa tataaaataa
    36481 ccaaacgata gaaaggctta ttctacatca cgtagta
//
LOCUS       DAZKLA010000017        35925 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000017 DAZKLA010000000
VERSION     DAZKLA010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.017"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..113)
                     /locus_tag="ACG85N_000675"
     CDS             complement(<1..113)
                     /locus_tag="ACG85N_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFQ0223471.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFII"
     gene            complement(115..834)
                     /locus_tag="ACG85N_000676"
     CDS             complement(115..834)
                     /locus_tag="ACG85N_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0223472.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            1382..1672
                     /locus_tag="ACG85N_000677"
     CDS             1382..1672
                     /locus_tag="ACG85N_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0223473.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNT"
     gene            1744..2970
                     /locus_tag="ACG85N_000678"
     CDS             1744..2970
                     /locus_tag="ACG85N_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0223474.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(3058..3450)
                     /gene="rpsI"
                     /locus_tag="ACG85N_000679"
     CDS             complement(3058..3450)
                     /gene="rpsI"
                     /locus_tag="ACG85N_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0223475.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(3466..3894)
                     /gene="rplM"
                     /locus_tag="ACG85N_000680"
     CDS             complement(3466..3894)
                     /gene="rplM"
                     /locus_tag="ACG85N_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFQ0223476.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(4428..4895)
                     /locus_tag="ACG85N_000681"
     CDS             complement(4428..4895)
                     /locus_tag="ACG85N_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFQ0223477.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(5015..6553)
                     /locus_tag="ACG85N_000682"
     CDS             complement(5015..6553)
                     /locus_tag="ACG85N_000682"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFQ0223478.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            6633..7319
                     /locus_tag="ACG85N_000683"
     CDS             6633..7319
                     /locus_tag="ACG85N_000683"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFQ0223479.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(7520..8047)
                     /gene="msrA"
                     /locus_tag="ACG85N_000684"
     CDS             complement(7520..8047)
                     /gene="msrA"
                     /locus_tag="ACG85N_000684"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0223480.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(8131..8808)
                     /gene="pgmB"
                     /locus_tag="ACG85N_000685"
     CDS             complement(8131..8808)
                     /gene="pgmB"
                     /locus_tag="ACG85N_000685"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0223481.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(8805..9935)
                     /locus_tag="ACG85N_000686"
     CDS             complement(8805..9935)
                     /locus_tag="ACG85N_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0223482.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(9935..12118)
                     /locus_tag="ACG85N_000687"
     CDS             complement(9935..12118)
                     /locus_tag="ACG85N_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFQ0223483.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(12115..13140)
                     /locus_tag="ACG85N_000688"
     CDS             complement(12115..13140)
                     /locus_tag="ACG85N_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0223484.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(13185..14012)
                     /locus_tag="ACG85N_000689"
     CDS             complement(13185..14012)
                     /locus_tag="ACG85N_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0223485.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(14009..15076)
                     /locus_tag="ACG85N_000690"
     CDS             complement(14009..15076)
                     /locus_tag="ACG85N_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFQ0223486.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(15102..15938)
                     /locus_tag="ACG85N_000691"
     CDS             complement(15102..15938)
                     /locus_tag="ACG85N_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0223487.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(15935..16804)
                     /locus_tag="ACG85N_000692"
     CDS             complement(15935..16804)
                     /locus_tag="ACG85N_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0223488.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(16874..18157)
                     /locus_tag="ACG85N_000693"
     CDS             complement(16874..18157)
                     /locus_tag="ACG85N_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0223489.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(18171..19856)
                     /locus_tag="ACG85N_000694"
     CDS             complement(18171..19856)
                     /locus_tag="ACG85N_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0223490.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            20131..21156
                     /locus_tag="ACG85N_000695"
     CDS             20131..21156
                     /locus_tag="ACG85N_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0223491.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(21751..25404)
                     /gene="rpoC"
                     /locus_tag="ACG85N_000696"
     CDS             complement(21751..25404)
                     /gene="rpoC"
                     /locus_tag="ACG85N_000696"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFQ0223492.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(25483..29100)
                     /gene="rpoB"
                     /locus_tag="ACG85N_000697"
     CDS             complement(25483..29100)
                     /gene="rpoB"
                     /locus_tag="ACG85N_000697"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0223493.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            29828..30796
                     /locus_tag="ACG85N_000698"
     CDS             29828..30796
                     /locus_tag="ACG85N_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0223494.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            30814..31719
                     /locus_tag="ACG85N_000699"
     CDS             30814..31719
                     /locus_tag="ACG85N_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0223495.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            31716..32522
                     /locus_tag="ACG85N_000700"
     CDS             31716..32522
                     /locus_tag="ACG85N_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0223496.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            32679..33647
                     /locus_tag="ACG85N_000701"
     CDS             32679..33647
                     /locus_tag="ACG85N_000701"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFQ0223497.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(33964..34488)
                     /locus_tag="ACG85N_000702"
     CDS             complement(33964..34488)
                     /locus_tag="ACG85N_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFQ0223498.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            34775..>35925
                     /gene="scm"
                     /locus_tag="ACG85N_000703"
     CDS             34775..>35925
                     /gene="scm"
                     /locus_tag="ACG85N_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0223499.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTS"
BASE COUNT        10354 a         7568 c         6640 g        11363 t
ORIGIN      
        1 aaaataataa aacaacctac tgttccagaa acaatcccta taactacaga tgtaattaga
       61 gcattctgta aaaagtgaaa gtcatacaga ccattaataa aattttgaag catattattc
      121 cccctctcca ataaatatag tatctccata ggcttttttt aaattttctt cattaaatac
      181 agattctaca cttccgtgag caattagatt tcgattcaaa agaataatat tatcaaaata
      241 cttctttacc ttgcttaaat catgatgcac aattagcact gtttttccct gatgtttcaa
      301 tttttttaac gtatccataa ttattcgttc actaactaag tctatcccta caaatggttc
      361 atctaaaaat atgaaatccg cattttgagc aagacatctt gctaaaagta cacgttgaaa
      421 ttgtcctcca gataattctc ctatttgatg attagaatat tttcccatat cgaccttttc
      481 caaagccttt gaaactcttt gccattctgc atttttaatt ctttgaaaaa ctttcattcc
      541 ggcatatgtt cccatagata cacattcttt tacttttatt ggaaaagtaa aatcaatatc
      601 agctttttgc tctacatatg caatcttcac cagtttttta ttgatatctt cattattaat
      661 ggagacctct ccctgatgag ggataatatt taacattgct ttaagcaatg tggattttcc
      721 agcaccattt ggaccaataa tacctgttaa agcaccttct tgtattttta aagaaatctc
      781 gttcaatgct aaaaaatcgt tataacaaac attaagcttt ttcacttcta acataacttt
      841 ctccttctaa atgataatga ttatcacgat catatggtat acaaaaatta tattttagtc
      901 aaccctaaat tttatagttt agattttttt ctgttgctga agattagaat gacaaaacta
      961 tttgaaacta cgcgtatagt aagtagcttt caaaatatat aaaaaaggag cgttctcaaa
     1021 tgagaacatg ctcctaaaac tactttattg gcgtatggaa aaattatact tgaagtggat
     1081 agcgggttga ataagttgat aagcatcaaa acatttataa aaattattca agactatgtc
     1141 tgttccatat acaaatatga aaatcagtaa actccattta acttttttcg tagtgtgtct
     1201 ttttatacat gtttatcgtt attaatagtg aataggaaaa aaactatttt tgatcattga
     1261 caattaaata caatttttga ataacgatcc ctgctattcg agctagaaac tcaagtacgc
     1321 catgacctga ttagcgacaa atacttagag atttcttttt gttctgggaa aacaaaatgc
     1381 catgacaata gcagcaaata atgatacttc cgtaaggctc gccgtaaact ggggagaggt
     1441 gaaattccta tgtgcttacc caaagcaatt caaaaatttc ataactacta ttttaggaga
     1501 tgattttata gaatccagag tctacctgaa taaaaaacaa acctgtgcat ttctaggaat
     1561 cgataataat actctagata agtggattcg tatatataat ttcccagtaa taagagttgg
     1621 tggtgtagtt cgctttgacg tagaagatat taaagacttt atgaatactt gaaattttct
     1681 tctcgccgca tatactaaaa ttagtgaaat atactgcctt acttttttta aggagtgagg
     1741 attttgccta caaagctttc aaatgggaaa tataaaacta atctgagata tccaaaaagg
     1801 tttaaagaaa taacaggaat tgcatcagaa aagtatcaaa aaacatttcc aaataggcaa
     1861 ttggcgatta aagccgaaaa tgatatgaag aagaaaatag aaaaagtttt gagagaggag
     1921 aatgcaaact cattagaatt aaaaggaaaa ataacgttta aaaaatttta cgaatctaaa
     1981 tggcttccta gatatgaatt aggtcaaaca attagatcaa acaggcctcc tagtgatata
     2041 accattagta atacaaaaga tatatttcgg ttgcatattt tacctatgtt tggtgaatac
     2101 gcaatgaatt atctaaatat caatactgag ataatatcag atgaattaac caaaaaatcg
     2161 aaagaatatg caaatataaa aatcataaaa ggatatgttc gttccatgtt cgatattgca
     2221 gaaatactaa attatatcga atttaatcga actactaaaa ttattcaaag tatcactgca
     2281 cctaaaaaaa atgctttaga agaaaaaaga atacaagaag gtaagcaagc actatcatca
     2341 aaagaactta ctaattggat agaagcagta aatgatgatt ttaataatca tttacttact
     2401 tttcatgatt acacactttt tatgattacc ctgtatcttg gtgatagaaa aagtgagact
     2461 tacgctttgc aatggaaata tattgatttt gaaaaacaaa cggtccgtct taaacatact
     2521 ttagataaat atcaaagaaa aaagtttaca aaaggtcgaa aagataccgt cattcaagta
     2581 ccagaagttg taatgacatt gctaagtgaa tggaaatcgg tacaagctga tcaattgttg
     2641 aaattaaaaa ttaagcaaac gttagatcag tatctcttta cttatactaa accatctgga
     2701 gaagtaaatt gtcctgttca tgccgattac ctaaactatc gaatcaattc aattaaaaga
     2761 aggcatcctg acttagttca tttatctcca cacaaactcc gtcatactta tgcaactata
     2821 gctagacaag gtggagcaga tatgaatcaa atttcaaatg ctttaacaca ctcagatatt
     2881 tctacaacaa agatatatgt gaacacacca gatatagttg ataaggctgt ttttgaagct
     2941 tttcagagag gattgaataa gtgtgattga tatgtgttct taaatgtgtt cttaaaaaca
     3001 aaagaagcac tttccaaata attgaaaagt gccagaaacg ttgatatata aaggatatta
     3061 acgttttgag aattgtgaag ctttacgtgc tttcttaaga cctggttttt tacgttcgac
     3121 catacgtgcg tcacgagtaa gtaagccagc acgtttaaga gctaaacgga agtcaggatc
     3181 aacttctagc aatgcacgag cgataccatg acggatagct cctgattgtc cagcgtatcc
     3241 tccgccgttt acgttaacaa atacgtcata tgcgcctttt gtttcagtaa cagcgaaagg
     3301 ttgattgatt acttcacgca agtcagcatg tggaatgtat tcttcaacat cttttttatt
     3361 tacagtaatt ttgccagttc ctggtactag acgtacgcga gcaactgcgt ttttacgacg
     3421 gcctgtgccg atatattgaa cttgtgccaa tgaaattccc tcctattaga ttaagtttgt
     3481 gatgtctaaa acttctggtt gttgtgctgc atgtggatgt tctgctccac cgtatacgtt
     3541 caattttgta aattgtttgc ggcctaaagt gtttttagga agcatacctt ttacagaagt
     3601 ttcgatcaaa cgacgagaat ttttagcacg caattcgcca gcagtgattg atttcaatcc
     3661 tcctgggtag ttgctgtgac ggtaataaat tttgtcagtc gcttttttac ctgttaattt
     3721 cactttgtct gcattgatga cgatgacgaa atcgccagta tccaaatgag gtgtgaaagt
     3781 tggtttattt ttaccacgta gtacagatgc aacaactgtt gaaagacgtc ccaatggaac
     3841 atcagttgcg tctactacat accatttacg ttctacttcg cctggtttgg ccatatatgt
     3901 tgtacgcacg ttattttcct ccaatttaag ttctgatgtt tgacatacac ttcgagtttc
     3961 cggggctctt tgtggggcaa acaataccat ttaacatatt acccgctaat ctagtcaatg
     4021 tcaacgtttt tctgcttgat tgaaaaattt atatgaaact ttacttcttt tctttctttt
     4081 atataaaagg gtcgtttcct gtcaaaatga aaaacaaaaa gaggctgtga caaaagtctt
     4141 gtcacaaccc cttattttga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
     4201 caatatccat aaaacataag aagctgtttt ttaaaattgt ttcttgtgac tcggagctga
     4261 acggcaggct tacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct
     4321 caaaaaacgg caaaatatga ggaactattt tgacgtttgt ttctctaata ctcaggtcaa
     4381 aacgctgatt tcacaacctc tttttcggtc aagttcaaca acagttttta cgcgtcgatc
     4441 tctggcgctt tgcccaaatg agcttggatc atccagatac gtttttctgt ggctgttttg
     4501 aattcgataa agatatcttg tgttggatcg tcgccttctt ctccagaaac ttcgattcct
     4561 ttttggtaaa gatctgctag ataacgatag cctgcaacaa gtgtttccaa acgttctgga
     4621 atagtacgat cccagcggcc aatttcatca gggatttttg tgttatctgc aaattcacgc
     4681 aatgtagaat aaggtgaacc gtctaatgtg atcaagcgtt cggatacttc atctaactga
     4741 gcgttcaaat catccatgaa ctcatccatc attgggtgca atgtaaggaa acctggtcct
     4801 ctcatgtacc aatgtgtttg gtgaacaaca acagacattt ggcttagatc tgcaactaat
     4861 tgatttaata cttctttggt ttgttggaat ttcatctttc ttgcctccta acattttctt
     4921 acacttaaaa taatagtaga ctttcaatga aaagtacaac aaaatcattt acctgagaac
     4981 gattttcagt tcgctttttt atccgtaaag ccaatcattt ttcagaatca gctaattctt
     5041 ctcgcagttg agcaaattgc tgataagtct gtgctaaatg cttttgtgtc gaaacaaata
     5101 atggatagat tttctgatat tttttatgat cagtttcttg tactccaact tgctctttca
     5161 attctatcaa ttcctgtgct tcactgatac tttggatctt tccgatgctt ctccagccta
     5221 aaatcacagc tcctaatgcg gatgcttctg tgacagtagg gaaaacaagc gggtaaccca
     5281 gtacatcagc catcatctga cgaaaaatcg gagattgagc aaacccgcct gtagcacgga
     5341 tctctgtgag ctgattttcc tggcaaactc cttttaaaat ccgataaagg ttgagacaga
     5401 tcccttctag aatcgccttc atcatttctt tttctgtatg ttgccgctgt aaaccgataa
     5461 aactgccaaa cgcatctgcg tcccaaagcg gtgctcgctc accaagtaaa tagggatgaa
     5521 aaatcagacc attggcaccc gctggcacaa aagcgatagc cgaaagcagt gcatcatatg
     5581 gattttctcc tttttcttta gcagattgaa cgacttcctg taaaaatgta tgccctgccc
     5641 aatcaaagat gccagcacca ttgctagtcg ctcctcccac tacccaatgt ttctgatcca
     5701 gaacataaca aaacgtctcc atttctggat ggaactgagg ctgagtagta ataaaacgaa
     5761 gtgctccgct cgttcctacg gtcaaagcgg cgatcgtatc accaaaagca cctaacccta
     5821 gattagaaag cggcccgtct gcccctccca aaacaaatga tatatcattt ggcaaaccta
     5881 gttctgctgc tgtttttgat ggtagacctg ttacttgata ggtggaagga aagacttgcg
     5941 gcaattgctc tattcgaatg ttcaagtagt caagcacttc ttctgcccat tggaattcat
     6001 ggatatcaaa atatccagtt cctgaagctg ttccgtagtc gctgatcagt tcgccaaaca
     6061 atctataaaa gatatagtct ttgataccga caaaataagc agtttttttg aataattcag
     6121 gttggctgtt ttgcagccac ttgattttgt tcaatggtgt cattggatga atcggcgtcc
     6181 cagtctgccg ataaaattct ttgccaagca cggtatattt cagtttttct gcttcgtccg
     6241 ctgctcggtt atctgcccaa gtgatcattc gagataacgg tgttttctct tcatccaaca
     6301 atatcaggcc atgcatcgca gaggagaagg agacaaattg aatgattgtg tctgaaggca
     6361 actgtttaat tacttctttt ataacggtgc agacaccttg aaagatcaat tcctgatctt
     6421 gttctgccat cccttgtgtt tcttggatca aagggtagcg aaaataagcg gatgctgctt
     6481 cttgggcttc atctgtgaaa gcaactgctt tggtttgtgt ggttccgata tctaatccga
     6541 ttgaaacgtt cattttttca cctcaaattt tatgtatgta caaaaattta gtttaagctt
     6601 gcgtatcttg gttttgaaaa gggggagatg gcgtgttact ttattttatc attggttgct
     6661 gtatcggatc atttttatgt ttaaccgccc agcggattcc gttaggtcac tctattattt
     6721 atcctagatc tcattgtgtc aaatgctgtc gttcgctttc ttggtatgaa ttgattccga
     6781 ttctctcgat catcgtccaa cgatttcgct gccgctattg caggtgtcgg ttgcccttct
     6841 actatttgtt ggcagaagcc ttatgtggtg gtctttttgc ctggttcttt actttttctt
     6901 ccgcaagaaa tttttcgacg ttcatatgga tgctttcggc tttgctattt tcgttgatgg
     6961 atttatttta cttcatggtc cattctcaca cgctattctg ttcttgggca attttatgga
     7021 ttttttggtt gcaggccggc gtcttccaat ggcaaagtgt gctgttaacg tttatcgtag
     7081 gcggtgtgtg ccttcgctat ggtcaatcct atctcggagc tggcgatact ctgttgatgc
     7141 tttcttggag tggtggcttg tctctagaag agttgcttca gatcttgttt cttgctagtc
     7201 tgtttggaat atccgttttt tctgtttact ttatctgtca taaaaaaaag ttggaaaagc
     7261 tgcctttcat tccttttttg agcagtgcgt tgctgattgt ccttcatctt aggtattgaa
     7321 aaaaggttgt gacaaacgac ttgtcacaac ctctgatata tagtgttctc ttaccaaatc
     7381 cttgtgactt ggagctaggc gacaggttca caacctctcc actaaatggt gttcaatcag
     7441 ctgaccctca gtattagctc aaaatgctgg ttttacaacc tctgatatac ggtgttccat
     7501 aagtagcttt ttcggcttct tatttcttga cgccccaatg tcgacgaagg aaacctgcac
     7561 gtccagaacc ttctcgataa agattgtaat ggagcttgtt tttcttgtaa taatcctgat
     7621 ggtaatcttc tgcaggataa aattcgcttc ttggttcgat ttttgtcacg attggcttat
     7681 cgaatcgccc gctgtcttgc aatgcttgtt ttgaacgctc tgcaatattt cgttcttctt
     7741 catcattata gtagatcact ggacgataag aatcaccgcg atcagcgaac tgtcctaatg
     7801 cgtcagtcgg atcggtttgc tgccaataga tttccaccaa ctgatcataa gaaataactt
     7861 ctggatcata ttcgatctct actgcttctg tatgccctgt tgtgcctgtt gttacttgtt
     7921 cataagttgg atttggtaca tgcccgccag tataaccgga tgtcacagaa atgatccctg
     7981 gctgctcttc aaatggtttg accatacacc aaaagcatcc gcctgcgaaa actgcttttt
     8041 ttgtcatata aaatccccct tttttctcat cataccttga tttccaaaag gaggataacg
     8101 ttttgcttgt gacagaaaaa acggaaacta ttatatacgt gatacggctt ctgataaatg
     8161 ccaaaacgaa cgtacttgat caaaaacggt taaatcagga atcacatatt cagcagaagg
     8221 aagatttatg ttttcaccta ttcccaatac atgcatgtta ccagccaaag ctgcttgaca
     8281 accagcttct gaatcttcaa tgaccaagca tgcatttggc ggtacattca attgctgtgc
     8341 tcctttgaga aaaacttctg gatcaggttt tgcttttgat acttgcgtgc cgtcaataag
     8401 gacatcaaaa taacttgtca aattcgtttt ttctaaaatg aggcgtgcat ttttgcttgc
     8461 tgatcccaat ccgatcttga tgtttgtttt tttcaagtac tcaagaacag ctattgcacc
     8521 aggcaaaacg gatgtttcat ccattttttg aatcgcttga agatatagat tgtttttttg
     8581 ctcagcaaag gctgatttct ctttttctga gaatttctgt tccatttttc caaatgacag
     8641 caatcgctct agtgactcgg ttctggatat tcctttcagt tgctcgtttt ctttctcagt
     8701 gaatggaatg gacagctggt tagctaaaac cagccacgct tcgtaatgat aacgagctgt
     8761 gtctacaaga acgccatcta aatcgaatag tgctgctttg attttcatcg attacctcct
     8821 tgaaaatgaa cgacttgaag taaagatgcg atgtttttag ctgtgcgatg aagattatca
     8881 gtggcttgct gtaaagcttc ttgtaaaggc acagctgcag gaaggatcgg aaaaacggcc
     8941 gaaatgccaa catctgctgc tttttcgctt cctttaccaa cacttccgca aatagctatc
     9001 acaggaacgc cctctggtac tcttctagca atcccaactg gcgcttttcc ttttagactt
     9061 tgtccatcta gacgtccttc gcctacgatt acgaggtctg cttgctgtac ccgtttatcg
     9121 aactccagta aatccaatac gtagtcgatg ccagagacta ttttccctga agcaaatgct
     9181 aacagtcctg cacccattcc accaccagca ctactgcccg atgcctcgat tacttgaggg
     9241 gcaaatctca tataaaaatc atgcatggct tggtcagcca ctgctaattc attttctttc
     9301 aagccttttt gtggtccgaa aacaaaagaa gcgccttctt ttccgcataa aagattagta
     9361 acatcagtaa tgacagtgat tttggcttta atcagctcaa taggcacgtt cgttttatca
     9421 actgtagcaa ttttacctag attttctccc agtgcttcta ctttttgatt atcttgatcg
     9481 taaaaagcat aacctagacc ttctgccatg ccgatgccgc catcgttggt actacttccg
     9541 ccaacgccga tgatgatgtg tcgaacacct tgttgcaaca attgtcgtat cagttctcct
     9601 actcctcgcg tggataacct aagtggctgt tgttggttta gtgggacatc tttcaatccg
     9661 acgatttctg ccatttcaaa aatcgcagtt tgaccgtctg aagcgtactg gatttctttg
     9721 tttttgccaa atgcattagt aacgactgct gtttgccgct tcaaccttaa actgtctgct
     9781 aatgatgaaa ccgttccttc tcctccatcg cctataggta gcagatcata ttcagcctga
     9841 acaaaaactt cttgaaaacc tgattgaatg gcttgtgctg cctctaaggc tgtcagactt
     9901 tctttgaaag aatctggcgc aatcagaata cgcattattt tcccctccgt tccaagatca
     9961 gttctttttc cagctggtat tggtctccat tgacaaaaat aggtaatgtt tctttcgtct
    10021 ctttttttat tactatttgt ttttttgtca aaacaaatag caacctgtga tttttccaaa
    10081 taaaaggaaa agaaattttt tcccatgctg aaggaagtct tggagaaata gaaagagttc
    10141 cattcgttac tgtaatacct gaaaaaccaa atataactgc tagccatagt gctcctaatg
    10201 aagcagcatg aattccgtca tctgatgaat gcggatctgg tcctaaatca atcaggcacg
    10261 cttcttggaa taaatgatac gcccattcca tttcttggat acggcaagcc tctatggcgt
    10321 gaatcgcttt actcaacgat gagtcgtgga ttgttttgtt ttcatagtaa tgcaaattct
    10381 tagaaacgat tggttgagaa aaatactcag gtaataagta taacagcatg acaagatctg
    10441 cttgcttgct gacttggagt tcatttactt cgctacgggc ataatcaagt aaaatagact
    10501 ggctgccagg acgttctttg taagtggtca aatcaataac tggcttactg agatacgtgt
    10561 catcttgcgg aatcagctgt tctttgttag gcactggcaa atataactta tctaaaaaat
    10621 tttgacattc tttcaaaaaa agcggatcgc cagcttgata tttaacagct aaggccacat
    10681 tataatgcgc cagatagttc gtatatgcat tattgtcgat atattctgtg tattcatctg
    10741 gaccaatgac tctgtgtatc tctaaatgat catcgacttt tacagctctg ctcagccaaa
    10801 aagctgctgt ttcatgaatg agcgtttttc cctttgtttc taaaaacgat tgatcctctg
    10861 ttgcttggac atactcttga acggcataag caaggtctgc actgatatga tgttctgcgg
    10921 cagctgaagc aacttcttgt cttgttccgg ttttgatatt caatgcagca aatttgggtg
    10981 tctcttcctg accagtgtgt gcgctttccc atgggaaaag tgcaccttga tagtgataat
    11041 ctcttgcttt ttgccgtgcg ccttctaatc gatcataacg gtacagcagc aaattctttg
    11101 ctacttcagg aaactgatat aaaaagaaag gcaggataaa aatttccgta tcccagaaaa
    11161 catgcccttt ataaccttcg ccagttagtc ccttcgcccc tacactaaca cttggatcat
    11221 tgcggggagt cataatctgt aaatggtaag cagcgaagtc catcgctaac tgatcgaaag
    11281 aagaatcgcc ttggatcgtt atccgatgct gatgccaaaa atctgtccaa taagacatcg
    11341 actgctctaa aatttctgta taactaacgc ttttctgggc ttgggcaata cttttcttaa
    11401 acgggtctgt ttgctgaaga gaggtagaga gcacgatgat cttctccagc gtaaaggacg
    11461 tattttttct gatcttttct gaaaaggaag cggtgatccg gcgatttttt gcttggaata
    11521 cacctgcttt attgaattta gctgcaatcc caatatcaat tttactttct gttgtctggt
    11581 aggatgcact gatcaaatcg tcaccaaata ctcgcagttc tttttcaacc aactgctgtg
    11641 taccaaaatt ggtttgctgt ccatcgatac ccgttttcac atctacttgg cagtcttcgg
    11701 tcaacggctt gatgaccact ttcagggctg cctgatggag attattttga tcagcaacac
    11761 gacaagtaat gacttccact ttttttcctt gagcagtttc aatgagcgca tagcgtttaa
    11821 gctctcccat ttctaaatct agtgcccgct cgtatgcaag aactttcatc gtctcaagag
    11881 aaaaatgttc accgtcgatt tttatttccc atctgatgac atctgggaga ttcactaact
    11941 cggctgattc tgctcctaaa ggtcggttat atacaccagc tgcaaacatt ccccttactt
    12001 gattccgata gtcttcctcc tggcttcctc tgataccaaa aaatccattt ccaatggtca
    12061 tctgactcgc atacttattg atatactcta aatcaaactg tttttcttct actttcataa
    12121 ggtaataact tctcctgttt ctgccgaacg atataaattt tccaccagat tctggatgac
    12181 atatccttgc cagccatctg caatcatctg agtctggtca ccgagcgccc gattcacaaa
    12241 atgagccata cttagttcat gtgggtcatt aactggcgtg gtcttttgta aggaaaccag
    12301 ctttccatct acatcttcga atagttcgcc tggatacaga ttagcacctg cctgatcgcc
    12361 acaaaaagcc acattcagtt tgtcttctct gatattcaaa gcaaacgaag tattcagttg
    12421 gatgatcttg ccgttttcta attccagtgt gccaaaaatt gcatcttcta ctgtgaattt
    12481 gcttggatcc catttcccga atttccctga attttttttc gtaccaattt tttggaagct
    12541 gtacgcccga acgcttttta ctttaggaaa tccagtaata tacatcgctg tatcaagcat
    12601 gtgaatgcca taatcgatca aaggcccgcc tccttgaagt tctttgtcga taaacgttcc
    12661 ccagcctgga acaccagacc gacgaagcgc attcgcttct acgtaataaa tatccccgag
    12721 aacatcaaga tgattcttga tcattgttgc ttcgtcagaa aaacgatgct ggaatccata
    12781 agccaaaatc tgcccgtttt tatcggctgt ctctgccatc tttcttgctt gatcagctgt
    12841 catagctggt ggtttctcgc acatcactga agcattagct tcgagggcag ccaatacatg
    12901 ttcgaagtga aagcgattgg gtgtacagac actcacgata tcgggagaaa tctcattcag
    12961 catttgtgta actgatgtat acgcatgaga aaatcctgta cgtcggcaaa agtcttctgc
    13021 tcgctggctg actggatcaa ccacagcggc caattctaag acatctgatc ttttttgata
    13081 ataagcagca tgtacttttt ctgctacttg tccagcgcct atgattgcta ctttcttcat
    13141 ttatttctcc ctcctttgct tatttactta ttgttattct ttctctattc tttctctaaa
    13201 tcatttaaat gacgcgctgt ttccagacag tttttcaagt attgcaatga ttcctgataa
    13261 attttcactt cattttctcc tcgcaaacga cattcatata ccatataacc ttcatactca
    13321 ttttgaagta gcgtttgaaa atggcgagta aaatcaattg cgccagatcc tggttggtag
    13381 cgatgattat ctgctaaatg gatatgaccc acatactctt tttgcgtagt caatgctttt
    13441 gaaatatcat cttcttcaat attcatatga taaaaatcag cgatgatctg cacatgtttc
    13501 aattgattag agtcaatgta atgacgcgca tccgctaaca aattgatcat atggtcttgg
    13561 tatcggttca aaggctccaa ataaataacc gtacctgttt cttcggctac tttatctaaa
    13621 taacgaagtg attcgctgac tgctttatag tctccttctt tgcttcgggg tgaaaccatc
    13681 ggcggtaatc gataagtgaa cattccccaa gctgctggga taacgattcc tttgcctcca
    13741 acctcgctta gtgcacgcaa aatctcagca atttgacgta caccattaag acgccgttct
    13801 tcgataaagt caccgatcca cccatcgtag ccgttacaag ctgtcactac tgggatccct
    13861 gtttctttga ttgcctgttt gactgtgtcg atattttcta ttaacaactt accgtcgatt
    13921 tcgtattctt caaatccaca gtctttgata aatctaaatt tttctaaaat gtctcttggg
    13981 aaaaatgctt gatcttgcgt accgaatttc attgttctcc tcctcggact tgaacgccca
    14041 ttttgatact ttcttctggg tgctggtcta cataacgcat gtaagcttct gcgctttctg
    14101 caaatggcac aactggatca atgatttctt cacaatccaa gtaaccgttc atcaataatt
    14161 cccaacatgt gtcctcgatt cgtttacggt tccatcttgg gtaatctggg tttggttcac
    14221 tagatgctcg agcaaagacg atttttgcat tattgaaatg tgcttcgcga ccaagattaa
    14281 acccagctgg aaaaggttta gcaaaggcaa cataagaaat gatcccgcca tagccgatcc
    14341 ctcttaaaga agcttgtaat gcggcagcac tgccactcgt ttcaataact gaatctactc
    14401 cacgtttatc tgtcaattcc tttagacgaa aaccgacatc tgctttcata ggatctagcg
    14461 tgtaatcggc accgtatttt tctgctaatt ctctacgctg cgctatggga tcgatcccaa
    14521 cgatggtagt agcacctgct tttttagcta attgaatcgc aatttgcccg atagcgccta
    14581 aaccgaagat tgctacagca tcacctgctc gaacatgtgc ttcccggata ccacctaaag
    14641 cgaattgagc aggatcgtaa catactgcat ttttccattg atccaaccga tcaagtttac
    14701 gtagtttata attatcaaca ccgtttgtaa tcagcgtttc acgaatgggt ccataagtgc
    14761 agactaactc tcctttttga tactctgtca ccaaactgcc cgtttcttcg atcgtaccga
    14821 caatcatatt tcctaattga aattcgccaa ataccacacc gcgttggctt ccttcttctc
    14881 ttggcaagaa taggcgccat tcttctgaaa attcttcatc gataaatgga cttaatcctc
    14941 taaagtcgac aatttctgtt cctggttttg gagtagcgaa aattactcgg atacgtactt
    15001 cgttcttttc gatctcacgg tcttcgtaat ctttgaatcc ggctattctt ggttctaatg
    15061 caactaattt tttcatcttt tctttcctct ttcttttctt tttatccttt tacgccgcct
    15121 tcgactaaat cacctttaat aaatcgttca gataacgcat acataatgat gactggtaaa
    15181 gcggtcacca atgaagcggc catcattcgt ccccaaatgt agtctggtgt actgaataat
    15241 gtgttcaaac cgattggtaa agtaaatttt tcggaggtcg acaggaaaat cgaagcaaat
    15301 aaataatcat tccacgaaac cataaagcaa taaacgaaga cagaaatgat tccagaaata
    15361 gccaatggta taatgatccg aataatgatc tggatccggc taagaccatc gatcattgct
    15421 gcttcttcaa tatcagtcgg aatcgtgtca aaataacttt ttagcatgaa tatcgcagta
    15481 ggaagtgttt gaacgaccat cgtgataatc agtgctgtat tggtatcata caaacctaga
    15541 ccggaaatga ttttaaataa tggaacaacg atcaaaatac cggaaaacat ataaacggtg
    15601 taaaagctag cattgatttt cgagcgagct ttgaagcgta gttttgacaa agcgtatgcc
    15661 ccgctgatcc caatcactac tgcagttacg gaggctatca aggagatcac taaactattt
    15721 ttgaaataca tgatgaatgg aaaaatctca gcattgaaaa tatcctgata atgttcaaaa
    15781 gtccattctt tcggaaaaat agtttgtgtc gttgcaattg cttcttgtga atccttgaat
    15841 gatgtcatca gcatgatcaa aaatggaaac aaattgacag ctaatagcaa caaaatcgtc
    15901 gcgtaaaata agattttcct gctcttttta tttttcattt caagtccacc ttctttcgtg
    15961 agagcaagac gaccgctgca ataatgatga acaatagaat cgatatagct gctgcttttc
    16021 caagatcatt gaacgcaaag gctgtctgat acaaataaac gcctaagata ttgactttat
    16081 tcgtcagtag atagacatct gtaaacatat agaacatcca gatagatcgt aaaacaatca
    16141 cagttgctag tactggtttg attgccggca gtgtcacgat cttgaacttc tgccaagcat
    16201 ttgcaccgtc catttcagct gcttcataaa gcgttttgtc gactgtttgc aaaatagcca
    16261 aaaatgaaat aaacgcatac ggatagtatc tccagatagc aaacaaaacg accataaaga
    16321 agctagcaac tggctggtcg aaccacagcg gtgcttcgtt gaaaagctgc aacctatcta
    16381 ctaacagatc attgacgatt ccataactat tattgaacat atatttccaa gcaaaaatca
    16441 aagaaataga tggcgtaaca taacttaata aaatcagtga acgcaccgtt ttcctaaatc
    16501 tgaacttgcg gttaagcaaa atagctacgg ctaatcctaa agctgtactg cctgctacaa
    16561 ctaagaatgt gtaagcaact gttaaaccta aagaacgata aaattcacta tcacttaaaa
    16621 tttcaagata attgcttaat ccgataaaaa cggcgtctga gttaggattc agcggcatat
    16681 cgaaaaaact gatctgaata ttggaaagca ttggaatcag cactaaaaca gctaataaaa
    16741 gcatgcttgg tgctaaaagt cccatggcta attttaagtc agaatgtttc cttggacgat
    16801 tcattgaaac ctccccctga taatctgcca atagctatct acttttttcg aacaccattg
    16861 gcagaacttc ttgttatttc gtattttttt gtgcgtcagc taaagcttga tcgacatctt
    16921 cgtttccgac agttacttgg ttcactgctt ttccgatcac acctgagctt gtgatatctc
    16981 ccattttcgt gaaattcttt tccccgacta gtccaaagac ttgtacttca tcaaaagcac
    17041 tagcaatctc agatggcagc tcgccaaaag acttgatgac ttcattttct ttgtaaccat
    17101 ccagttcgac tacttgttta ttcactggtt gagcaccacc aggagacatc aagacccact
    17161 tttccatgtt tttcggttga gataagaaag ctgtgaatgc ttcagcagcc tctttttgtt
    17221 tctcgtctag accagaagaa atcgtcaatc cagatactgt gccatagaca gcttgatctt
    17281 tttgtgttgg gatagcaaaa ccgatatttt ctgagttccc cgcttcataa acagaaggca
    17341 aaatgtaagt tgaatagatt gccattggtg ctgttccatt catgaaagca tctttgattt
    17401 ctgtcacatc attagagcct ggcatcgtgt attgggataa gttttgataa aacgacaatg
    17461 cttctctcat cttttccgta tcgatcgtta cctcgccttc atcatccaaa acatttgctc
    17521 catttgacaa agcaaattga gagaacgctt gctcactcat tgttccttca gcagtaggca
    17581 tagcaattcc atattgttta ttctctttat ctgtgaaatg ttgagcaatt tttaaaatat
    17641 catcccaatt ttctggttct gaaaaaccag cttctgataa tttttctttg tcataccaaa
    17701 taccttgaac ccagccgcta attggtgcag caatatagct ttttccatct tctgaacgaa
    17761 caaggttttt ggctccgtca taataattat cttctccagc ttcctcgata acgttttgga
    17821 ctgccgtctg atcaatcagt tcatctttat ccatcacttt agcaaaatcc tgactgactt
    17881 ccattacttc tggtaattta ccggaacgag ctaacgtaac gatttttgta ttgaacgctg
    17941 attcttctac cggcacttgc ttgattttga tgtctggatt ttctttttca aaatcagcaa
    18001 ccagttcatt gatcacactt aatcgttcct gttcgacaga agagtgcatg aattgaatcg
    18061 ttgtctgttg tgattcgtta gagctttgac tttcaccaca agcagtaaga agaaatccag
    18121 tagctagcag acaggtagta acaataattt ttttcatttt tttccacctc ttattttatt
    18181 attttcagcc acatatattc atacggctgg aatatcaact ttcgttcttg actgcaatgg
    18241 ataatttgtc cagaaaacag gtctaggtaa tctccttttg gtaaaagata acctgctgat
    18301 tgattggaaa aattatggat aactaagact ttctcttcct tcgcttcgcg aataaaactg
    18361 ataaaagaat ctgctgagtc cagtaagtaa atcaaactat tcggatggaa ggctttttct
    18421 ttttttcgaa tagatagcat ctgggtcaat tcaccatata ctgctgttct taatggatct
    18481 gtctgcaact ctttctcaat atctgctaac aaaaattttt gccgattgat ttttcgattc
    18541 tctcctgttt tttctacgcc tttgtaatca tttcgacttc ccagcacact ttgaatataa
    18601 attgccggca cgcctggtaa cgttaccaaa aatgaatgag cagctaaaaa gcgtttgctt
    18661 ctccattccg tcgtatctgt tggagaactt aacgcatcta gatacgtagc gtttatctca
    18721 tatggactgg tcgttccatc tgggtttttc ttgtagttta ctcgggtacc ctctttcttc
    18781 agctgttcca ctaattcaag gatcgttttt tccggcagaa tacctcgtac cggattcaat
    18841 ccgataccgt catgggaggc taaaaaatta aaaaaagttg tttttttcga tgcgaaatcg
    18901 atggttttta accattcttt caacttactt gtattacccg aacgaatcgc ttgaagggtc
    18961 aaaggtggta gcgaaaactg atagaccatc tgtgcttcat tctcgccatt cccaaaataa
    19021 ctgatattat ctttgtgtgg cacatttgtc tcagtgatca ataccgttcc tggagctgct
    19081 tcatcagcga ttgcacggaa taattggatg attttatgcg tttctggcag gtgtatacaa
    19141 gaagttccta gttctttcca taaaaatccc acagcgtcca agcgaataaa tgaagcgcct
    19201 ttttctaagt aaaacaaaag aatatcgatc attttcaata aaacttctgg atttttaaaa
    19261 ttcaagtcta cttgatcttc gctaaaagtc gtccatacgt gtttttcttt cccgtttttg
    19321 tcaatgaacg ttgtaagtaa cgggctggtc cttggtctag tcacgctgct tgtatctgta
    19381 tccagtggca aatcaataaa aaaatctttg tacgtatcat tcaagtttaa atattcctga
    19441 aaccaagtac tttcggcaga catatgattg cagacaaagt cgaacattaa ccgtgtttcg
    19501 cttctcattt cttctatatc ttcccatgtg cccaaacggt cgttgattgc ctgataattt
    19561 acaacggaga aaccatcatc agatgtatat gggaaaaatg gcaagaaatg tacaactcta
    19621 aatgtatctg ccaaatactt ttgataaaat ttcttaaatg tacgcaaagt tggttgctgc
    19681 tcttcaaaaa attgatcccc ataagtaatc aagaaaatat cttcttcgtc ccaatgcagt
    19741 ttgcctgcct ggcctttctt ctgctgataa acactgactt tttgttcaat catagaggcg
    19801 gttttctcag acaaatctga gtcgcgataa atcgcttgta aaagttttgt atacatcgga
    19861 ttcccctttc ttttttggga gcgttcccaa cgatttgatc atattatgga aaacgcttac
    19921 tgtcaatagc atttttttaa ataattttga tatacacaaa aatacaaata agaaagctta
    19981 tgtatcaata attaatagct tcttaaaaaa atatggtctc tcatgatcat gcaagctatt
    20041 cctaaatttt caaaatcata gacaaaaaag aatgtctatt gtactatgga attattctaa
    20101 aacgctccca aaaaggaggt gcatataaaa ttgaaaccta ctatttatga cattgcgaaa
    20161 aaagctaatg tctcaaaatc aactgtctcc agagtattaa ataaccagcc aaatatctct
    20221 gataaagcaa aagcaagtgt acttcaagca atcaaagaat tgaattatga accgagcaaa
    20281 attgcaagag gactgtcagg aaatggtttt gacgcgattc tagcattatc tcatcgtact
    20341 tctcatacga cctatggcaa tccttttttt tcagatgtta ttcaatccat ttctgccgtt
    20401 tctgaacaaa ataacttcga cttgattcta caaacggcaa aaacaccaga agaagagcta
    20461 gaaaaatgcg cgaaaaagat ccaagaaaaa atgatccgag gaattattat tttaagctct
    20521 acgatggatg aaacacttct ttttgaattg gatcaataca atattcctat tgtggtaatc
    20581 ggaaaagtcg gaaacgacta cacgaacatc tactcagtgg acacggataa tttccaggat
    20641 agttacgatt tggtggaaac actgatcaag aatggacacc gtcatatcgg ctgtctccat
    20701 gctccgacag aagtttatgt atccaatgat cgtctcaatg gttaccgtca atgcttattt
    20761 gatcaccacc tagatatccg taatgaatgg attatagacg gcggctttcg cttagaagat
    20821 agcatggcag caatgaatga tttaatgaag ctacgagaaa gaccaactgc gattttttct
    20881 accgatgcat taaaatcatt gagtatttac aaagtggcag aacaatctgg aaagcagata
    20941 cctgatgatc tttcgatcgc aggattcagt gatcccttat tgtcctccct attttcgcca
    21001 cagttgactc ggatagacat tccgacaaat cagttaggaa tcaaagccac agaactttta
    21061 ttccaactaa tccaccacca ataccctgaa gaaaaaagaa tcattctttc aaccgagatt
    21121 actctcacag attcgatcca acaacaagaa atgtagaaca gaattataaa agaacagaaa
    21181 cactgaggat aagtcagtta ctctgttctt ttttcaatca ctcatccctc gttaccccct
    21241 cccggtattt tagcgaattt ttcttatttt ccatctgttt tagatagctg tggataacta
    21301 taatgtttgt gttaaactat tttttccaca gggaagaaaa aactattttt aaccacgttg
    21361 tgtataagtt tttatagttc gtttttttaa acacatttac ccttatttca atccatgtta
    21421 tttggggata cttatctcta aaacataaat agaataacag acatgtctac tggttttgtt
    21481 ttattcgagg aattattcaa taatagctag caaaatatta aaaataaccg ctctatcttc
    21541 ccatcaattt attttgcatt caaaaaatga tctatcttta cttttgttga ttgacttctc
    21601 ttggaagatg atcgttccga taccttaatt ggatacgaac cgatgttgtt aagatccttt
    21661 cccacttttc ttctagttag acaaattatt ttagacaaac caaaaaagag gttgtgacaa
    21721 taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca ttgcgtcttc
    21781 tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg ctacaccgac
    21841 ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga tgatcttacc
    21901 tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga tcgcagcatc
    21961 cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat ttgtttctaa
    22021 tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc ctcctgagac
    22081 tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac ctggtaagat
    22141 atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa ccattacttc
    22201 gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta cttcacgaag
    22261 caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg gatcgattga
    22321 tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt tcatacgtgc
    22381 tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta cttctttggt
    22441 acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg tgatcactgc
    22501 ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac cttgagtgat
    22561 atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag taccaggttc
    22621 accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt cagagccagt
    22681 tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac atgtgaagac
    22741 agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga tgtcttcgga
    22801 aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta cttctttacg
    22861 ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc cctcgcggat
    22921 ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga ttacatcttg
    22981 ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca aggctgtatc
    23041 ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg atagtccttc
    23101 acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg ccatcaaacc
    23161 acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag aatcagacat
    23221 catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga tgctgtcttt
    23281 tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga tcagaccacg
    23341 acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga tttcttgttt
    23401 ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg tcgaatgttt
    23461 gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt tgaatcgttt
    23521 gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata ctaattcttg
    23581 ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt ccggtgtttg
    23641 gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag gcatgatctc
    23701 gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc attcagtaaa
    23761 tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat gaacatatcc
    23821 gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt cgccttcgcg
    23881 tccttcttct tccattgtca agtagtagtt acctagaacc atatcttggg aaggagtaac
    23941 aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca acatacgtgc
    24001 ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc catcgaaatc
    24061 ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt gtactaaaac
    24121 tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca gcaatactgg
    24181 gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac gttcgatttt
    24241 acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac gcatcacgaa
    24301 tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca tcttcaagaa
    24361 aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta gcaagttttg
    24421 acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg ggcggttacc
    24481 tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta ctgcttcttg
    24541 cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta acaaacgttt
    24601 caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac ttgttgcaaa
    24661 acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag ggataacgtc
    24721 cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa tatccaaacg
    24781 gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt tcaattcggc
    24841 tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg cacccatagc
    24901 agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt ctgtcaacaa
    24961 ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag caaaataaat
    25021 gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg aaggaatccc
    25081 tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca tgcgttcacg
    25141 gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc ctttataacg
    25201 gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa aaatccgctc
    25261 acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct caggtttttt
    25321 tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta tttgcatact
    25381 ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat ggcaaaccgc
    25441 aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta aattatcttc
    25501 tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt cagcagcttt
    25561 cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat caacagtgat
    25621 caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt cgatatccaa
    25681 gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga atgattccgg
    25741 tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt tcacacgtcc
    25801 gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg caccgtaagc
    25861 ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag ctttacctcc
    25921 caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca atttgtcatc
    25981 gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc catcaaatgg
    26041 ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac cggcttcacg
    26101 aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg ctacgtggat
    26161 acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga tgttcatacg
    26221 tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag gtaggaatgg
    26281 catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac gaccggccat
    26341 tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta acatgtttac
    26401 gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt gaacgatacc
    26461 gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt tttcgccaaa
    26521 gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg gcgtgacttt
    26581 ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac ccatttcgtc
    26641 taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt cttcaggtcc
    26701 taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag tgtatacgtc
    26761 atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc cttcccaagt
    26821 catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca tagatggtcc
    26881 gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg gacgttggtt
    26941 gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat ctaatgcacc
    27001 gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac cgtcatgttt
    27061 acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag taccaaccca
    27121 tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac ccatcaaggc
    27181 acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg caactacttg
    27241 tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt tttcactttg
    27301 agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg ggctgtttgc
    27361 ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt ggtcagtaac
    27421 acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga atttatttac
    27481 tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag gagtttcgat
    27541 cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac cggcgcggtc
    27601 acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca attcgccaag
    27661 agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt ctttgatact
    27721 tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag tatcttggat
    27781 cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga attggttttg
    27841 taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat catcgacatt
    27901 gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt cggctggacg
    27961 aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt cacgttcagg
    28021 gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga ccacaccgtc
    28081 ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag ctagtgtttc
    28141 catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg tttctggatc
    28201 aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta attttttgtt
    28261 tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga aacgagcatt
    28321 cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac gttcgtaaac
    28381 gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt ctttttcgac
    28441 tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat cggaaccaaa
    28501 tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga tacgtacata
    28561 agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag ggataacagt
    28621 agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga cacctggtga
    28681 acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg ttccttgttc
    28741 tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc cagtctcgcg
    28801 gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat gcgcacgagc
    28861 ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca aagatagatt
    28921 gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc cctcatctaa
    28981 gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca acacttcact
    29041 gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt gtccagccaa
    29101 gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt tacaataata
    29161 tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg caaaaaaaaa
    29221 ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt gtttccttat
    29281 attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat agtttttgca
    29341 acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt tcttctgctt
    29401 tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga caattcttaa
    29461 ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga ggaacttcta
    29521 tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac tttagctact
    29581 ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt tgtatctatg
    29641 atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct aagtggctga
    29701 gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta ttttccgact
    29761 tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa ttaaggagga
    29821 aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac tagctttagg
    29881 aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa caattggcgt
    29941 attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca aagaaggatt
    30001 agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa atgcccaaaa
    30061 caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc ctgatttgct
    30121 acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca ctatcccgat
    30181 gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca atgaaaaacc
    30241 tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa tcggcttact
    30301 gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg gtgaagcaaa
    30361 ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg tcaaagtcaa
    30421 agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc tagctaaaga
    30481 tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa gtgttgtcgg
    30541 tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc aagtcaaaga
    30601 cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggtaaacaaa cagggatcat
    30661 ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa cagcgaatga
    30721 tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc cagacagcat
    30781 caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc tcttttctta
    30841 tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata cgtcactttc
    30901 cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg tgcagggatc
    30961 gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct aggcttttta
    31021 gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa aattcccgcc
    31081 cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg tgtgatgggt
    31141 gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga gtcattcggc
    31201 ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat cgttgtatgt
    31261 ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac tggcgataat
    31321 ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat cggttatatg
    31381 atttctaatg gatgcattgc tctttctggc tccttgcttg cgcaaaacaa cggctttgct
    31441 gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat catcgcagaa
    31501 gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt aggtgcggtc
    31561 atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag tgattcgaaa
    31621 cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca gcaaaaattc
    31681 catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta acattggaaa
    31741 atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg aaaggcatta
    31801 atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg gctggtaaat
    31861 ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa attttattga
    31921 atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc agtcgcgtat
    31981 tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat cttgctcttg
    32041 ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa gaccgaacat
    32101 ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta tcctcagaaa
    32161 tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc acattacagc
    32221 gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct acttccaaaa
    32281 cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct tttatggtca
    32341 ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg catcaaggaa
    32401 ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc caattattag
    32461 cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt ttatcttcat
    32521 aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt ctctttcctt
    32581 ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt gttctctcct
    32641 tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa gacaaggtac
    32701 ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca cagcaactgg
    32761 aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca ggctatcgtt
    32821 ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat gcaaaagaaa
    32881 tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg ttgacttctg
    32941 aatctacgat gaaagatgtc aaactggcag cagctcaagg agattctgtt tcgaagttat
    33001 ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt ttcgctgaac
    33061 ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt gatgaattgc
    33121 ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct gtaaccggtc
    33181 gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa gtctgtggga
    33241 ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc atcattggga
    33301 tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa gaaaaagcca
    33361 tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc agcaaaatct
    33421 ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc tataaagaaa
    33481 aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact tatgtcggtc
    33541 ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa cagaagatga
    33601 gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta tcagatttca
    33661 caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca aaagactctc
    33721 cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta aaaatatcta
    33781 taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag tcatcagttt
    33841 acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata gaccatgggt
    33901 aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt tttagctagt
    33961 gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag agtgcatagc
    34021 agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg tccagttctc
    34081 gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa tagcaaaatc
    34141 agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga tctgctttgc
    34201 ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg gaaaccggct
    34261 ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa tctggaaatc
    34321 taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg gggctctatc
    34381 ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac gttcaaaccg
    34441 atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt attttcctcc
    34501 tttttattta tcctacttac tttttcctaa ttctaggata cctacttata tgttactaca
    34561 cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga ttgaaagaaa
    34621 aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt cattaatatt
    34681 ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa aagagtataa
    34741 taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa gtttactagt
    34801 cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag cggaaacata
    34861 cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg gcttgttagg
    34921 aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg cacatacaga
    34981 cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag agcttcatga
    35041 aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg ctaattcttc
    35101 aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta acgtggatgt
    35161 agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact taactccaga
    35221 tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg aatttgctaa
    35281 attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat atactgctgc
    35341 agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag ataccgggtt
    35401 tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc aaatcattaa
    35461 tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat tgaacgtaca
    35521 gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt cggatgcgaa
    35581 tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc cttttcaagg
    35641 aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg gaacgattat
    35701 aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg aaatatttat
    35761 cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat ctaccacaag
    35821 tgatagctca gattcatcaa cttcaagcga cagtacagat actacctcta ccacaagtga
    35881 tagcacggat acatcagctt caagcgacag tacagatact acctc
//
LOCUS       DAZKLA010000018        34603 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000018 DAZKLA010000000
VERSION     DAZKLA010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.018"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACG85N_000704"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACG85N_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFQ0223500.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACG85N_000705"
     CDS             complement(2020..2922)
                     /locus_tag="ACG85N_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0223501.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACG85N_000706"
     CDS             complement(2919..3611)
                     /locus_tag="ACG85N_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFQ0223502.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACG85N_000707"
     CDS             complement(3654..4295)
                     /locus_tag="ACG85N_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFQ0223503.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="ACG85N_000708"
     CDS             complement(4333..4647)
                     /locus_tag="ACG85N_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223504.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACG85N_000709"
     CDS             complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACG85N_000709"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFQ0223505.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACG85N_000710"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACG85N_000710"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFQ0223506.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="ACG85N_000711"
     CDS             complement(5984..6916)
                     /locus_tag="ACG85N_000711"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFQ0223507.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="ACG85N_000712"
     CDS             complement(6913..7689)
                     /locus_tag="ACG85N_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFQ0223508.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACG85N_000713"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACG85N_000713"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFQ0223509.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /locus_tag="ACG85N_000714"
     CDS             complement(10933..12009)
                     /locus_tag="ACG85N_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFQ0223510.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="ACG85N_000715"
     CDS             complement(12014..13294)
                     /locus_tag="ACG85N_000715"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFQ0223511.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="ACG85N_000716"
     CDS             complement(13313..14239)
                     /locus_tag="ACG85N_000716"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFQ0223512.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="ACG85N_000717"
     CDS             complement(14252..15535)
                     /locus_tag="ACG85N_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFQ0223513.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACG85N_000718"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACG85N_000718"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFQ0223514.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="ACG85N_000719"
     CDS             complement(16323..17234)
                     /locus_tag="ACG85N_000719"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0223515.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACG85N_000720"
     CDS             complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACG85N_000720"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFQ0223516.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="ACG85N_000721"
     CDS             complement(17700..18398)
                     /locus_tag="ACG85N_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFQ0223517.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="ACG85N_000722"
     CDS             complement(18385..18771)
                     /locus_tag="ACG85N_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223518.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="ACG85N_000723"
     CDS             complement(18918..20588)
                     /locus_tag="ACG85N_000723"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFQ0223519.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="ACG85N_000724"
     CDS             20771..21004
                     /locus_tag="ACG85N_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0223520.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="ACG85N_000725"
     CDS             complement(21103..21534)
                     /locus_tag="ACG85N_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFQ0223521.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="ACG85N_000726"
     CDS             21613..22026
                     /locus_tag="ACG85N_000726"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFQ0223522.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="ACG85N_000727"
     CDS             complement(22064..23212)
                     /locus_tag="ACG85N_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0223523.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="ACG85N_000728"
     CDS             complement(23334..23930)
                     /locus_tag="ACG85N_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0223524.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACG85N_000729"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACG85N_000729"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFQ0223525.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="ACG85N_000730"
     CDS             27160..27591
                     /locus_tag="ACG85N_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0223526.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="ACG85N_000731"
     CDS             complement(27636..28985)
                     /locus_tag="ACG85N_000731"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0223527.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="ACG85N_000732"
     CDS             complement(29055..30218)
                     /locus_tag="ACG85N_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0223528.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="ACG85N_000733"
     CDS             30347..30670
                     /locus_tag="ACG85N_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223529.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="ACG85N_000734"
     CDS             complement(30853..32202)
                     /locus_tag="ACG85N_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0223530.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="ACG85N_000735"
     CDS             complement(32271..33224)
                     /locus_tag="ACG85N_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFQ0223531.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="ACG85N_000736"
     CDS             complement(33326..34516)
                     /locus_tag="ACG85N_000736"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFQ0223532.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9829 a         7584 c         6082 g        11108 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttt
//
LOCUS       DAZKLA010000019        34448 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000019 DAZKLA010000000
VERSION     DAZKLA010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.019"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..1041
                     /locus_tag="ACG85N_000737"
     CDS             352..1041
                     /locus_tag="ACG85N_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0223533.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACG85N_000738"
     CDS             1028..2236
                     /locus_tag="ACG85N_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0223534.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACG85N_000739"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACG85N_000739"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFQ0223535.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG85N_000740"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG85N_000740"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFQ0223536.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="ACG85N_000741"
     CDS             complement(6339..7034)
                     /locus_tag="ACG85N_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFQ0223537.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG85N_000742"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG85N_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFQ0223538.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="ACG85N_000743"
     CDS             complement(8233..8424)
                     /locus_tag="ACG85N_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFQ0223539.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="ACG85N_000744"
     CDS             complement(8445..9335)
                     /locus_tag="ACG85N_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFQ0223540.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="ACG85N_000745"
     CDS             complement(9322..10089)
                     /locus_tag="ACG85N_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0223541.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG85N_000746"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG85N_000746"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFQ0223542.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG85N_000747"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG85N_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFQ0223543.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG85N_000748"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG85N_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFQ0223544.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="ACG85N_000749"
     CDS             14660..15043
                     /locus_tag="ACG85N_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223545.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG85N_000750"
     CDS             complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG85N_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFQ0223546.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="ACG85N_000751"
     CDS             complement(15987..16805)
                     /locus_tag="ACG85N_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFQ0223547.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG85N_000752"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG85N_000752"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0223548.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG85N_000753"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG85N_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFQ0223549.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="ACG85N_000754"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="ACG85N_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFQ0223550.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="ACG85N_000755"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="ACG85N_000755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFQ0223551.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20824..21066
                     /gene="yaaA"
                     /locus_tag="ACG85N_000756"
     CDS             20824..21066
                     /gene="yaaA"
                     /locus_tag="ACG85N_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFQ0223552.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21053..22177
                     /gene="recF"
                     /locus_tag="ACG85N_000757"
     CDS             21053..22177
                     /gene="recF"
                     /locus_tag="ACG85N_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFQ0223553.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22174..24120
                     /gene="gyrB"
                     /locus_tag="ACG85N_000758"
     CDS             22174..24120
                     /gene="gyrB"
                     /locus_tag="ACG85N_000758"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFQ0223554.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24140..26611
                     /gene="gyrA"
                     /locus_tag="ACG85N_000759"
     CDS             24140..26611
                     /gene="gyrA"
                     /locus_tag="ACG85N_000759"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFQ0223555.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26847..27146
                     /gene="rpsF"
                     /locus_tag="ACG85N_000760"
     CDS             26847..27146
                     /gene="rpsF"
                     /locus_tag="ACG85N_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFQ0223556.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27196..27708
                     /gene="ssb"
                     /locus_tag="ACG85N_000761"
     CDS             27196..27708
                     /gene="ssb"
                     /locus_tag="ACG85N_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0223557.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27734..27970
                     /gene="rpsR"
                     /locus_tag="ACG85N_000762"
     CDS             27734..27970
                     /gene="rpsR"
                     /locus_tag="ACG85N_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFQ0223558.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28113..30086
                     /locus_tag="ACG85N_000763"
     CDS             28113..30086
                     /locus_tag="ACG85N_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFQ0223559.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30092..30544
                     /gene="rplI"
                     /locus_tag="ACG85N_000764"
     CDS             30092..30544
                     /gene="rplI"
                     /locus_tag="ACG85N_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFQ0223560.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30818..32185
                     /gene="dnaB"
                     /locus_tag="ACG85N_000765"
     CDS             30818..32185
                     /gene="dnaB"
                     /locus_tag="ACG85N_000765"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFQ0223561.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32965..34257
                     /locus_tag="ACG85N_000766"
     CDS             32965..34257
                     /locus_tag="ACG85N_000766"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFQ0223562.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10510 a         6702 c         6821 g        10415 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    20761 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    20821 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    20881 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    20941 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21001 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21061 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21121 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21181 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21241 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21301 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa
    21361 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21421 agtaaaaggc agcccgcaga tcaggcggaa gtttttagat atggaattag gccagatcaa
    21481 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21541 cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21601 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21661 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21721 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    21781 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    21841 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    21901 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    21961 taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22021 tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22081 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22141 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22201 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22261 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22321 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22381 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22441 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22501 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22561 gttgtaaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22621 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22681 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22741 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    22801 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    22861 ggcgggatca aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    22921 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    22981 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggtgga
    23041 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23101 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23161 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23221 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23281 ttcttgctgg aaaacccaag tgtgggccgg caaattgttg aaaaaggatt actagcttcc
    23341 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23401 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23461 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23521 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23581 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23641 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23701 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    23761 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    23821 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    23881 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    23941 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24001 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24061 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24121 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24181 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24241 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24301 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24361 catgggtaaa taccatcctc atggagacat tgcgatctat gagtcaatgg tacggatggc
    24421 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24481 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24541 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24601 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24661 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24721 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    24781 tggaccagat tttccaacag gtggtttggt tatggggaaa tcaggaatcc gaagagcgta
    24841 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    24901 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    24961 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25021 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25081 cgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25141 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaatcc ttgaaaacta
    25201 tatcgaacat caacgcgaag tcatcacacg acggacggtc tttgaaaaag aaaaagcaga
    25261 agcaagagca cacatcttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25321 tgcgattatc cgcgggtcta aatcagatga cgaggcaaaa gcaacaatga tcgaacggtt
    25381 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25441 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25501 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25561 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25621 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25681 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25741 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    25801 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    25861 tccagaatac gggcgtacag ccaaaggaat cccaatcatc aatttactag gtatcgattc
    25921 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    25981 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26041 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26101 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26161 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggtcagag ggatacgcct
    26221 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaaaatcaag aagtattagt
    26281 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26341 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26401 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26461 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26521 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26581 aacagttgaa gaacaaggat cagaagaata accatcaaga ctaagtgtgt aagctagcaa
    26641 gaataaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26701 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatta
    26761 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    26821 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    26881 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    26941 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27001 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27061 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27121 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27181 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27241 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27301 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27361 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27421 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27481 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27541 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27601 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27661 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27721 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    27781 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    27841 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    27901 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    27961 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28021 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28081 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28141 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28201 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28261 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28321 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28381 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28441 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28501 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28561 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28621 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattagatg
    28681 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28741 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    28801 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    28861 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    28921 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    28981 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29041 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29101 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29161 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29221 tcgttatcaa tgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29281 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29341 aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc
    29401 tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc
    29461 caagtaaacc tctgctgact tatattgaat cttcagcttc ttcagcttca gagctagttt
    29521 ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat
    29581 tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa
    29641 cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt
    29701 tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact
    29761 atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta
    29821 tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca
    29881 ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg
    29941 tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac
    30001 tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata
    30061 tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa
    30121 aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct
    30181 aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca
    30241 aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga
    30301 attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg
    30361 tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat
    30421 caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa
    30481 cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga
    30541 ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa
    30601 ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca
    30661 gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata
    30721 aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct
    30781 tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg
    30841 aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc
    30901 agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca
    30961 tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt
    31021 tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta
    31081 tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat
    31141 cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa
    31201 aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt
    31261 ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc
    31321 cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac
    31381 tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct
    31441 tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg
    31501 aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt
    31561 aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct
    31621 ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat
    31681 ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact
    31741 agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg
    31801 taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact
    31861 ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca
    31921 gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga
    31981 tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga
    32041 cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg
    32101 aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc
    32161 gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc
    32221 tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga
    32281 tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt
    32341 caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac
    32401 gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg
    32461 tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt
    32521 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac
    32581 ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct
    32641 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc
    32701 acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa
    32761 gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt
    32821 tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat
    32881 aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta
    32941 aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc
    33001 gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg
    33061 taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg
    33121 catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt
    33181 gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa
    33241 acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg
    33301 gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc
    33361 ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat
    33421 aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta
    33481 aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac
    33541 ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat
    33601 gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga
    33661 acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt
    33721 gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc
    33781 ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta
    33841 ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta
    33901 gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat
    33961 gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta
    34021 atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa
    34081 ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac
    34141 gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct
    34201 gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga
    34261 taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac
    34321 gataataata acttgaagag gtagtgaaag agacgtttag ccttattgcc gataggctgt
    34381 ctcttgaaca ctgtttattc agaataaggg actgtgacaa aaaccgtgtc acagtccctt
    34441 tttccgaa
//
LOCUS       DAZKLA010000020        32606 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000020 DAZKLA010000000
VERSION     DAZKLA010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.020"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(161..1210)
                     /gene="recA"
                     /locus_tag="ACG85N_000767"
     CDS             complement(161..1210)
                     /gene="recA"
                     /locus_tag="ACG85N_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFQ0223563.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFANPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1319..2560)
                     /locus_tag="ACG85N_000768"
     CDS             complement(1319..2560)
                     /locus_tag="ACG85N_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFQ0223564.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2800..3378)
                     /gene="pgsA"
                     /locus_tag="ACG85N_000769"
     CDS             complement(2800..3378)
                     /gene="pgsA"
                     /locus_tag="ACG85N_000769"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFQ0223565.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3587..4510)
                     /locus_tag="ACG85N_000770"
     CDS             complement(3587..4510)
                     /locus_tag="ACG85N_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFQ0223566.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(5249..6535)
                     /locus_tag="ACG85N_000771"
     CDS             complement(5249..6535)
                     /locus_tag="ACG85N_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0223567.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6528..7796)
                     /locus_tag="ACG85N_000772"
     CDS             complement(6528..7796)
                     /locus_tag="ACG85N_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0223568.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            8048..8509
                     /gene="mscL"
                     /locus_tag="ACG85N_000773"
     CDS             8048..8509
                     /gene="mscL"
                     /locus_tag="ACG85N_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFQ0223569.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8568..9014)
                     /locus_tag="ACG85N_000774"
     CDS             complement(8568..9014)
                     /locus_tag="ACG85N_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0223570.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(9107..9352)
                     /locus_tag="ACG85N_000775"
     CDS             complement(9107..9352)
                     /locus_tag="ACG85N_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFQ0223571.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(9467..10045)
                     /locus_tag="ACG85N_000776"
     CDS             complement(9467..10045)
                     /locus_tag="ACG85N_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0223572.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(10122..10652)
                     /locus_tag="ACG85N_000777"
     CDS             complement(10122..10652)
                     /locus_tag="ACG85N_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFQ0223573.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(10656..11516)
                     /gene="rlmB"
                     /locus_tag="ACG85N_000778"
     CDS             complement(10656..11516)
                     /gene="rlmB"
                     /locus_tag="ACG85N_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFQ0223574.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(11509..11904)
                     /locus_tag="ACG85N_000779"
     CDS             complement(11509..11904)
                     /locus_tag="ACG85N_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFQ0223575.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(11901..13313)
                     /gene="cysS"
                     /locus_tag="ACG85N_000780"
     CDS             complement(11901..13313)
                     /gene="cysS"
                     /locus_tag="ACG85N_000780"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFQ0223576.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(13310..13846)
                     /gene="epsC"
                     /locus_tag="ACG85N_000781"
     CDS             complement(13310..13846)
                     /gene="epsC"
                     /locus_tag="ACG85N_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFQ0223577.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(13932..14171)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14168..15628)
                     /gene="gltX"
                     /locus_tag="ACG85N_000782"
     CDS             complement(14168..15628)
                     /gene="gltX"
                     /locus_tag="ACG85N_000782"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFQ0223578.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(15798..16955)
                     /locus_tag="ACG85N_000783"
     CDS             complement(15798..16955)
                     /locus_tag="ACG85N_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFQ0223579.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(16989..18359)
                     /gene="radA"
                     /locus_tag="ACG85N_000784"
     CDS             complement(16989..18359)
                     /gene="radA"
                     /locus_tag="ACG85N_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFQ0223580.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(18422..18949)
                     /locus_tag="ACG85N_000785"
     CDS             complement(18422..18949)
                     /locus_tag="ACG85N_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFQ0223581.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            19179..19985
                     /gene="proB"
                     /locus_tag="ACG85N_000786"
     CDS             19179..19985
                     /gene="proB"
                     /locus_tag="ACG85N_000786"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFQ0223582.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            19982..21229
                     /locus_tag="ACG85N_000787"
     CDS             19982..21229
                     /locus_tag="ACG85N_000787"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFQ0223583.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            21305..21649
                     /locus_tag="ACG85N_000788"
     CDS             21305..21649
                     /locus_tag="ACG85N_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223584.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(21766..22092)
                     /locus_tag="ACG85N_000789"
     CDS             complement(21766..22092)
                     /locus_tag="ACG85N_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223585.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(22707..23909)
                     /locus_tag="ACG85N_000790"
     CDS             complement(22707..23909)
                     /locus_tag="ACG85N_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0223586.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            24026..24541
                     /locus_tag="ACG85N_000791"
     CDS             24026..24541
                     /locus_tag="ACG85N_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0223587.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(24740..25186)
                     /locus_tag="ACG85N_000792"
     CDS             complement(24740..25186)
                     /locus_tag="ACG85N_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0223588.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(25233..26711)
                     /gene="msr(C)"
                     /locus_tag="ACG85N_000793"
     CDS             complement(25233..26711)
                     /gene="msr(C)"
                     /locus_tag="ACG85N_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0223589.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(27065..27694)
                     /locus_tag="ACG85N_000794"
     CDS             complement(27065..27694)
                     /locus_tag="ACG85N_000794"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0223590.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(27678..28142)
                     /locus_tag="ACG85N_000795"
     CDS             complement(27678..28142)
                     /locus_tag="ACG85N_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0223591.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(28629..29897)
                     /locus_tag="ACG85N_000796"
     CDS             complement(28629..29897)
                     /locus_tag="ACG85N_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0223592.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(30128..30865)
                     /locus_tag="ACG85N_000797"
     CDS             complement(30128..30865)
                     /locus_tag="ACG85N_000797"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0223593.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(30932..31501)
                     /locus_tag="ACG85N_000798"
     CDS             complement(30932..31501)
                     /locus_tag="ACG85N_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0223594.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            31629..32501
                     /locus_tag="ACG85N_000799"
     CDS             31629..32501
                     /locus_tag="ACG85N_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0223595.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
BASE COUNT         9126 a         6982 c         5864 g        10634 t
ORIGIN      
        1 acttttctat taaattattc tttgtagatg aacagatatt tttattttat gtacccactc
       61 gtataattaa ataactcacg atttctttat tcaataaaaa aacggcaggt caaggagagt
      121 acctactctt cagaccagcc gtttttgttt atcataattt ttattcttct actaaaggca
      181 attcttcttg tcctttttca tcttctggga cagcgacttc ttcaccaata ccataagctg
      241 ctctaactag agcagaaact tctgccatca tttcaggatg attagccata tagattttag
      301 cgttttcccg tccttgacca atccgatctt ctttataaga ataccaagca ccgcttttat
      361 caacaatgtc tttttctact gccatgtcta gaagttctcc ttcttgtgag atccctaaac
      421 catacatgac atctacttca gcgattttga aaggaggtgc tactttgttt tttacgactt
      481 tgatctttgt acggttacca acaatatctg taccttgttt caattgttct gcacgacgta
      541 cttccaaacg gatcgttgcg tagaatttca atgcgcgtcc tccaggagtg atttctggat
      601 tcgcaaacat cacgcctact ttttcacgga tttggttgat gaaaatagcg attgtttttg
      661 ttttgttgat cgaaccagag agtttacgca atgcttgaga catcaaacgt gcttgtaacc
      721 cgacatgtga gtcacccatt tcgccgtcga tttctgctcg tggaactaaa gcagcaactg
      781 aatcgactac tacgatatct acagccccac ttgatactaa agcatcagcg atctctagac
      841 cttgttctcc tgtgtcaggc tgtgaaagaa gtagttcatc gatattcaca cctaattttt
      901 gtgcatattg cggatctaac gcatgctcag catcaatgaa agcggccgtt ccgccatttt
      961 tttgtacttc tgcaatagcg tgtagtgcaa ctgttgtttt acctgaactt tcaggtccat
     1021 atacttcgat gatacgtcca cgggggtaac cgccgacacc taaagcgaca tctaacgcaa
     1081 gagagccact tggaattgta gagatttgtt ggtcgatttt ttcgcccaat ttcataatag
     1141 agcctttacc atagctcttt tcaatttttt tcagtgcagc atctaaggct gctttacgat
     1201 catctgccaa tacgataatc ctccttatat ttacaaatca gagcaacatt tcattatcct
     1261 tatcatatct tttttagtga aaaaaagcaa gaaaaaaccg aacaaatatt cgcatttatc
     1321 attttcttaa aagctgtttt ctcaaaagat caaatccttg cataaccgca ttatatctga
     1381 tagcagcacg atctctggca aagtgatatt tttccgaaac cacgccgtct ttagatgaaa
     1441 tagcgatcca tactgttcct gcagattgac cttctaacgt gtccggtcca gccactccgg
     1501 tgaaagctac actgtaatct gttcttgcta tctcacgtgc ttgaatggcc atctgctcca
     1561 cacattcttt gctgactgtt ccatattctg ctaatagctc cggatcaatc tccagaaaac
     1621 cagcttttgt ttggagcgag taggtaacga atccgcctgg aaatacttca gatacaccag
     1681 caatgtcgcc taatgcggct tggaaggcgc cggctgtcaa actttccgct gctgttacag
     1741 tctgcttgtt ttctttcagt agttccacta cgacttttgc tagcgaatta tcatcgccgt
     1801 atccataaaa atattcccct actcgttctt ggattttttc ttctaatgct aaaagagctt
     1861 gattccctgc ctgaacatca tttgttttca ctgtcagtcg taatgtcact tcatttggtt
     1921 tggcatatgg tgcgatcgtt ggattgatct gtgtctcaat cagatctttt agctcagtga
     1981 ccagtcgcga ttcaccaatc ccataaaagc gcaaaacttt agaaattagt ttttcttcag
     2041 aaggaaaacg ttcctctagt aaaggacgta cttgttccac aaacattggt ttcaactcat
     2101 taggcggacc gggaagaagg atatacgcat gtgtatctgt ttgataaaaa gtccctaaag
     2161 caagccctgt acgatttggt agaggaaccc cgccttcgat aatctgggac tgttgcagat
     2221 tgtttttggt catttttcga tgggttgttt caaaatatgc caataatttt ttgtatcctt
     2281 ccgtattttg tatgagagac ttaccgaggt gagccgcagt tgcttctttt gtcaggtcat
     2341 cttctgtcgg tcctaatccg ccacataaga gtatgagttc acttcttgtt tcagctagag
     2401 caatcaactc ttctaaacgc tgctgattat ctcctacaac tgtttgataa taaacttcga
     2461 tacctagatc agctagctgt tctgacaaga aagtggcatt cgtattcacg acttgtccta
     2521 gtaaaagttc tgttccgaca gctattattt ctgctttcat tacttcccgc tcccttatat
     2581 agtatacgct gtatttttat atttttttat ttaaaaagag ggcaaggagc tgtgacaaaa
     2641 atcttgtcac agctccttgt tccaaataaa cagtgttctc ctgccaaaac ctcggtcata
     2701 agtcacaata tacataaaac atgaaaagct gttttttgaa attatccctt gtgactcaga
     2761 gttagacggc aggctcacaa cctctccact aaacgatact tacattgatc ctttgaacac
     2821 accgctgttt ttgatgaagt aatccacacc tgaatagata gtaaagatca gacaagcata
     2881 aagcatgatt tgatctactg gaataccgat caggctgaat gggatattat tgataaacag
     2941 caagataata gcaaccattt gtgtagccgt cttgactttt cccggccaag ctgcagccat
     3001 gacttctcca tgttcgacaa gtagtaaacg caagcctgtg acggctaatt cacgacagac
     3061 aatgatcgct actacccaac caggggcctt tccttggtca accaacatga taaaagcagt
     3121 cataactagc attttatctg ccaacgggtc cgcaaacttg ccaaaattag tgacaaggcc
     3181 tcgcgcacgg gcaatttttc catctaacca gtctgtgata ctagctaaag caaaaataac
     3241 tgcacctact agctgtgtga cagccagtgt ggtgtctcct acgttaaatt gtccccaatc
     3301 taaaggtaaa cctacaacca aaatgaaaat cgggatcata caaattctaa gtacagttaa
     3361 tttatttggt aaattcacac tatcgactcc tttcttccct attctacgtg actatcagcg
     3421 aagtttccac cgatattatt tacttttcca aatgaattta ttcaaaggag tatttttatt
     3481 gaaattgatc tttttgaatt aacgaagaag gaccgcgaca cagatttgtc acgatccttc
     3541 ttttttatac ctacaagtaa acagatgcgt aaactaattt atacgatcat tctgcgtatt
     3601 gaagagtaag gttcacattt tttggactct gattgttttc ccctggatta actggtacgg
     3661 attctccatt cgctttgatc gttgcattgc ttgctgcacc aatcgtgatg gtcgcatttg
     3721 ttgctgtttc cggtaacacg gtactctgtg tttcatttgc tgccagtgtc ccttgataaa
     3781 cataggcatt gtctactagc acacctaacc atacacgatt gacagcagta aattcaaggg
     3841 ttacaggctt ggtagcgtct gtgatattta ctgcgatggc actgccagaa tcttctcctg
     3901 tagctattgc cattttcttt tcttcacttg attttgttgt tgattctact gtagaagact
     3961 ctttggtctg cgtgctttgc tctgaggaag aggactcctc gactgatcct tctactttaa
     4021 gcgaagaagc tgtgtctccg attattggat cagcttgacg atcttgccaa gtcatatagc
     4081 ccacgacaac gacaatcgcc aaagcaacca atcctaataa aatgacagga agacttgtcc
     4141 aaaagcgact tggatgcgat tcttcctgat gcattgcttt tctagaagta cttactgttt
     4201 caggctgcgg acgttcaggg accgatcctg tcaatttgct tttcccatca aaaacatcga
     4261 ctaaatggtc tccatcttct cctacagctt cagcgtattg ccgaataaat gcccgcacgt
     4321 aaaatttacc aggcaattgg tcaaaatcat cattgtcgat tgcttccaaa taacgttttt
     4381 gaatttttgt aatctgctgt aattcatcta aagatatttt tttattcagg cgtgcttgcc
     4441 gtaatttttc tccgatggtt tcaaaagcca ctcgttcatc ttctccagtc tgtttgattc
     4501 tccgaagcat ctgctgttgc aacgaaagac gttatgtttg ggtgctgtga caaaagtcat
     4561 gtcacagctc cttttttccg aataaacatt gttctcctgc caactcctcg gtcacaagtc
     4621 acaatatcca ataaatataa gaagctgttt tttgaacttg ttccttgtga ctcggagtta
     4681 aacggcaggc tcacgacctc tttcttaaac ggtgttcaat cagctgttcc tcggtattag
     4741 ctcaaaaacc ggcaaaatat aaggagatat ttttccgtat gtttctctaa tactcaggtc
     4801 aaaacgctga tttcacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa
     4861 taagtccaac caagttaaaa acattaagaa gctgttttta cttggctgtt cttattactt
     4921 gatgcagggc agctttttca caacctcttg gtacacggtg ttccagaagt agcttcttcg
     4981 gcaataagcc gaaatatcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt
     5041 cttgaagctg accacttctg tcacaacctc tttgattact tcgtttgatt ttattttaat
     5101 ataagttgat taaaagagag catgatttgt tttattttct cactaatttt tccaacacaa
     5161 ctaagaatac cataaaagct gattttggag gaggttccta ctacaagtct aaaacgaaat
     5221 ttaagacttt tcctcataaa atactggttt attttggtct catgtaaaac tcgctgaagg
     5281 cctcttcact tataaatgct tccccagctg ccagtacgtc tttcatttgg atcgaatcga
     5341 tgatttcagg aagatcaaat aaagttcgat cgccaaataa actttgcgta aattgattgg
     5401 caatatattc gatcgaattt aatgattgga aatattgtcc aagcattttc ttcttcagca
     5461 aatcgagatt tgtttctgac acttccggat catcagcgaa tccaagaatg atttctttga
     5521 ctttttctgc tgctttctct ggttcgtccg tatctccgct gaagtccgca aagtgaaatt
     5581 cacgatccaa gctgaattca aaaccaaagc tgtcatcgat aattccttga ttgtacatcg
     5641 ctagatagtt tcgagaagta ttgcctaaaa tcatttggaa caacagattg attgctgttt
     5701 tatagcgtaa cagttctgtt ccttcttgcg gcaatgtgtc taatccttta ataccaagta
     5761 caaatttatc tcgtgtaatg gctgcttcca atgcactttg tttgatgatt tcttttgtgt
     5821 tttcagggaa ataacgtaca atttcttgct ttggagggaa attttttgct tcttggtttt
     5881 cccgaataag tttcatcaat ttctcaggct ccatcttacc aactacaaat aaaaccatat
     5941 tgcttggctg ataaaacgtc cgatagcacg tatacaagtc ttgagctgtg attttgtcga
     6001 tactttcaac cgtgcccgca atatcgatat gcaatgggtg tgtaggatac aagttattta
     6061 gtatgccaaa aaacatccgc cagttagggt cgtcttcata catttggatt tcctgaccga
     6121 tgattccttt ttccttgttt acagtttctt ctgtgaagta aggtgcttgt acaaaatcga
     6181 tcagagtcgt cagattcttt tctacctgat ccgtcgtaga aaacagatag ctggttttcg
     6241 taaaacttgt gaaagcattg gcggaagctc cttgtttccc aaataattga aagacatcac
     6301 catcttcttt ttcaaacaac ttatgttcta aaaaatgggc aattccatct ggaactttca
     6361 ccttttcttt ttcaccatag ggaataaatt cgttatcgat cgaaccataa ttcgtactga
     6421 acaaaccata tgtcttgtga tagtcatttt taggcaacaa atacacggtc aaaccgtttg
     6481 gcagtacttc atgataaagt gtttcattga tttgctcata ttctgtttta ttcatttgca
     6541 tttcctcctg ctaaaaagaa gattgcctgc aattcgattt gctcagctac ttgttgtatc
     6601 tctttgatcg ttacgctctc catacgtttc aaccattctt catcagataa ttttgtttcc
     6661 ggcagccagc tgtccaaaaa agaagcttcg attgccgctt gcggagagtc tagagaaagc
     6721 agatattggt ttctcagcat tgcttttgtc tgagacagtt ccaactctgt tatttcacca
     6781 tttcgcaaac tttctaattg ctcgtgaatc agatgaagta cttgatttcg gttcttttca
     6841 tcgataccgg tttggacact catgaatcct cggaacgtat ccacgctact agaggcataa
     6901 taagctagac tttctttctc tctgacattc atgaaaagtt tagagtgagg aaaaccgcca
     6961 aataaaccgt taaagaccat tagcgcaaaa cgttctggtt catcgtaata cacatttgtc
     7021 tgataagcta ggttcagctt tgcttgaacg attggctcac gtacttgtcg ttccttcacg
     7081 atattcactt gaggttgggt ataaaacaga tctggataaa ctgtttcccg cgactcaaat
     7141 ggtaattgcc caactgcttc agctgctttg tccggatcaa catcgccgac aacaaaaatg
     7201 tcgatttgat catttgccat catttcttga tagtaggacg ctagcgaact agcagtgatc
     7261 ttgtccaaat cttcgactac gcccaaccct gggatctttt gttccgggct gttttcaaaa
     7321 taaagttctt gcaaggcata ggaagcaaaa gtttgtttat cttccttcaa actttccaaa
     7381 tatagacgca gattgttctt ttccaagtca aacgtcaact gatcaaactg atgattttta
     7441 atatttggat aaaataaaac ctcttttaaa aaatctaccg cttgagaaaa aaggtttgga
     7501 tcattgacat attcaccatt cacaaaactg atccctgcgt ttagccaatg taaatttcct
     7561 ttttttctaa ctgataatcc aaagcttgcg ccataaagct ctgcaagttg cgcacttaac
     7621 tttgtctgat ccgaataatt caaactattt gtttccaaca tgctgctgag aagtgaacgt
     7681 ttcgtgatga cttcttgttg cagacgcgca gtaaaacgga taaaaatccg gatcgttttg
     7741 tattgttttg ttggtaagac atgaagattc acgccttctg ccaattgata caccatattt
     7801 atatgctcct tttcacagtt gattgcccgc ttactctttt aatgggtcaa cctgcctttt
     7861 ttacttattc ctctgcttga aatttataag ttcaggcaat tcgccgtttt ttctaaatga
     7921 tgttacactt tatagtatag catagatgtt ttttatcaaa agaaacagac ggctaaaagc
     7981 gtatattgtt tttttatttt ttatttttcg atatactaaa gacaaataaa agaagggagg
     8041 gtcattcatg atcaaagaat tcaaagagtt catcatgcgt ggagatgtct tggatcttgc
     8101 tgtcggcgtt gttattggta gtgcgtttac aagcatcgta aaccaagtgg tgcaaggctt
     8161 gatcacacca ctaataggtt ggtttgtcgc aatgatcaca ggtacttctg atttagaagg
     8221 agcactttca attcttgact ggtcgccgac aaaaggcgtc acatttgcgt ttggtaacgt
     8281 tattagtgct atcattacat ttctgatcac tggttttgtc ttattcttag ttgtcaaagc
     8341 ggcaaaccgt gcgaagaatt tacgctccaa agaagagcaa gtggaagtgg aagaaactcc
     8401 tactgcagaa gattatctta gtgacatacg cgatcttctt gccaaacaag tggcagaaga
     8461 caatacacat ccagtagagc aaacaaatct ttccgatact tctaaataat aaagaatcta
     8521 aaaaaaagaa agccgatttt cagtgtcggc tttctttttt tagattctta acttgctact
     8581 tgttcctttt tcatgtcgat atgtggaata tcatcttcca aatacacatc tgagattgcc
     8641 ttgaatccaa acgactcata aaaagcttgt aggtattctt gtgcgccgat cacgattggt
     8701 ttttctggaa aatttttctg gatcatgtta atcgtttctt gaacaatctt tcttcctaaa
     8761 ccatctttac ggttgtcctg atgaacaagg acacgtccaa aatggatact ctccgcttct
     8821 tcatatactc gcgtgtaggc aaggatcttc ccatcagtat cttgaaaaaa cgtatgccaa
     8881 gcttctccat caatgtcatc aacttcttga taaggacact gttgttccac aacaaaaact
     8941 gcgattcgtt ctttgacgat cgcgtaaaac tctgctgttg acaattcttc aaatttcttt
     9001 acctgatatt ccactaagca aacgccccct gcttttttat tcttagcata ccgattcgca
     9061 cacaaaaaag caaacgaacc ttacgaatct caggctcgtt tgctttttaa actgcttcag
     9121 ttaaaaattc gatctcaact gtccgagtaa gtacatctga atagctgtaa gatactcgtt
     9181 caaaagaatt ttcttcagga tccaaatcaa caacgaatac tgaaggatag gtttctgtta
     9241 aaacgccgcg gcgttctgtt tgacgctttc ttcctgtctg agcaaccaac atgattgggc
     9301 tgccgatacg accttctaaa tctttcttga ttgaagctaa agttgttggc atacagttca
     9361 cctctactat caactatacc acatattttt aaaattttca agtttaccat aaaaaaaaaa
     9421 gaaatgcaat aaaaataaat aagaagctca ttaagatttt tctaaattat tcctcattat
     9481 aaaatttcaa gtgttttttt attttgagtt ttactctccc gtaagcgctc attacttgct
     9541 tgtgatccat tgctaaacat tcagccatgt ctacaaaatc aacgccttgc atataataat
     9601 gaaaaatttt agcttctatt tttgaaaact ggatttcgca attttcgatt gcttctttta
     9661 ataaaagatg ctgaattgct aaaggttctt gcaatgtgat ctctgctaaa aagtctggtc
     9721 cttctgtctc tattttttgg tcgattgaga cttcatcttt ttgatcacga cgcttcagcg
     9781 cgttctgctt cctcaacaga gaatagattt gattgcttat aacgctttta taataacttc
     9841 caaagctaac acctttgctt ttttcgtaag ttctcaatgc ctgatataaa gcgatccttg
     9901 cttcttgttc ccaatcctca gaaacataat ttcttaaata ataggtgttt ttcaaacgta
     9961 gtaaaagtgg aaaatatttt tgatacaggg cagcaaatgc ttcttcgtca ccattacgtg
    10021 cagcgcgcaa taactcctca gacataaaat aattcctcca taaattcgtt tatggaggaa
    10081 tcttatcatc aatgattcat cgaactgatt agaacgatat gttatttatt ttttctcatt
    10141 aaatcgtcca atttttggga caattttttt aactgctctt cattccatgg agaattacgt
    10201 cggaaatcaa taaaatgcat atctttattt tcttgggcta ttgttttttc cgtttttttt
    10261 atcgtcttat ataattcata agcagaagtt cgtaatgctc cttgggaaaa gatcatccac
    10321 tgttctgcta aatcgcttgt cgccactgtc acttgtgtca aacggtcatt cagctgacca
    10381 gcgattctct cgatataact gtcagccgtt tcgtcttctt tcgtaaaaac aacatccaac
    10441 tggtatttct tataattttg ttggatgccc ggaactagtt gggcatcgaa gacaacgatg
    10501 acttccattc cttcatattt cgcataattt gataaacgat gtaaaagagc ttctcttgca
    10561 tccgccattt tgtcctgctt tttaagctgg acaagttccg gccacgaacc aatcatgttg
    10621 tagccgtcta caatcaacag ctgttttttc atggtctact ccccctgacg ttgggaaaaa
    10681 gcctgataca taagcaaacc agcagcaaca ctggcattta agctctggac gtgcccggtc
    10741 atcgggatcg tcagcaattc atccatttct tttttcaatc ctggactcat tcctttgcct
    10801 tcattaccaa tgactaaagc aattgatcct ttggcattcc atttttggaa gtttgtgccg
    10861 gtcatatctg tgccaaatac ccaaaatcct gtttctttga gctgttgcac agcttgtacc
    10921 aaattcgtca cgcgtgcaac agggacatgt tcgactgccc cagtggaagt ctttgttacg
    10981 ataggtgtga ttccaactgc tcggtgtttt ggtatgatca caccatcgac gccacttgcg
    11041 tctgctgtcc gtaaaatcga cccaaaattg tgtggatctt cgatgctatc caaaatcaaa
    11101 aagaaggggt cttctttttt ctcttttgtt cgttcaatca gttggtcaag ggataaatat
    11161 tcataaggtg taatcgcaag aacgactcct tgatgcacgc cgtgatcgct caaagtttct
    11221 aatttttgtt taggaaccca tttgactggc acagcttgtt cacgtgcgat ttctttcagt
    11281 tcttctactt tttctcctcg tgaatcttct tgaagaaaga gtttattccc tcgcccttgt
    11341 tgtagcgctt ctactgcggc atggtgccca aagacgaaat tatcttctaa tgcttcttca
    11401 ttttgatcag aaactgactg atttcgtcca gtttgctttt tatttgaata tctattttgt
    11461 tttttgtctt caaatccacg tttcgattga tgtttgtttg ttctttcttt acccattttt
    11521 ttcaccaact ttctgaatac accagcggat caattcttcc atccgctcgg tttgttttgt
    11581 caaatgcaga tagcccatga gcgcttcgaa ccctgttgcg acccgatacg tcgtaacatc
    11641 agcatttttt gcgctcgtat ggcttttagc atttcttcct ctgcgataat aaagttcttc
    11701 ttcttctgtc aaaagatgtt cttccagcat ttgctgaatc aaaaaggctt gtgcattcgc
    11761 agaaacataa ttcgttgccg cacgatgcag cttattgggt tttgtttctc cttgatcaat
    11821 caggtggtca cgaacataga tttcgtaaat cgcatctcca acataagcca aggctaaacc
    11881 gttcaattgt gtgtaatctc tcatgcttct cttctccatc gggttccttg cggtgtatct
    11941 tctaataaga tccctttttc tttcaacaaa tcacgaattt cgtcgcttcg gacaaaatca
    12001 cgattgcttc ttgcttgatt tcgttcttcg atcaattgtt caatctcttc atctaataac
    12061 tcttctgctg taaataaaat accgaagatc gaaagccatt gcgagaataa agcttctcct
    12121 tgtgccaaga cagcggcaga aactttcggc tggtctagat aagtgttcag ccacttagca
    12181 agctcataga ccactgtgat cccatttgct gcattaaaat catcgtccat ttcttcgatg
    12241 aatcgctgtt ctaaattttc taacaaagcg attttatccg catcatctgc caaattctct
    12301 gaggcagttt cttggcggaa tttcaaattg tcaaacgtat tcttcagtcg ttgtaaattg
    12361 gtggctgctt ctttcatcgt cgtttcactg tagcggatcg gacgacggta ttgtgtggtc
    12421 gccatgaaga aacgcaagac ttgtggatca acgtttttga tcatttcatg tacagtgatg
    12481 aaattcccta gagatttact cattttttca tcgtcttcgc caatcgttac atagccattg
    12541 tgcatccagt aattagcgaa ttttttccct gttttcgctt cactttgtgc gatttcattt
    12601 tcatgatgag gaaattctaa atcttgcccg ccaccatgga tatcgatcgt ttctcctaaa
    12661 tgttttgttg ccataacgga acattcaata tgccaaccgg gacgtccttt gccccatggt
    12721 gaatcccaag aaatctcgcc aggttttgct tgtttccata aagcgaaatc taatgggtct
    12781 tctttttgct gctgttcttc ccctgtacgc tgacttgctc cgacttccag ttcatcgatg
    12841 gattggtcac ttaatttccc ataattttca aatttacggg tacggtaata cacatcacct
    12901 gaagattcat aagcatagcc tttttcaacc aacacttgaa taaatgctaa aatatctggc
    12961 atatgatcca tgactcttgg atgcagtgta gcaggttcga cattcaacgc tttcgtatct
    13021 tcttcaaatg ctcggatgaa acgatcagca acttctggtg ctgtaatacc taattcgttt
    13081 gctgtacgga tgattttatc gtctacatcc gtgaaattcg atacgaaatc gactttatag
    13141 cctcgatatt caaaatagcg tcgaattgta tcgaaagcaa tggtgctacg agcatttccg
    13201 atatggatat aattatacac agtcggtccg cagacataca taccgacatg tcccttttgt
    13261 atagggatga attcttcttt ttttctggtc aatgtattgt agatttttat cattctgatc
    13321 tcctcacttt ttctcctttg attcgaacga cttttgctgg tactcctacc gctgtagcgt
    13381 cgtctggaac atctttcaac acaacggcac ctgcaccgat tttggcgttt tttccgatcg
    13441 ttaccggtcc taatatctgc gcattcgcag agagcatggc tccttgttta acagttgggt
    13501 gtcttttacc cgtttctttt cctgtgccgc ctaatgttac gccatggaat aaaatcacat
    13561 cgtcctcgat ttctgcggtt tcgccaatga cgattcccat tccatggtcg atgaacacgc
    13621 cagttccaat cgttgctcct ggatggatct caacaccagt taggaaacgc cagaattgag
    13681 cattgatctt cgccaataaa aagagccggt gctgatataa aaaatgagag aaacgatgcc
    13741 aaaacaaggc atgaaaacca gggtatgtca aaacgatctc agctgtcgaa cgtgcagccg
    13801 gatcgtttct ctttgctgca tcaatcgctc gggtaaacca actcatgact ttccactcct
    13861 ttcgtaagaa ttttatgtag gaaatcatct tcttataaag aaaagaagcg ccttttaaat
    13921 aagtttcctt atcaaaagac gctttccgcg tggttccact tttcttcata atcaatgatt
    13981 atctctgaca gctaacgcct gttatacgtt cttggctact ttgtttccta taaaacagtt
    14041 tttcctgttt tctgtgtttc gccaagacca ctcccagagg catttcggca accattcatt
    14101 gataccttgc accacccggt atctctcttg taatgatcag ctgcttactt ttttctgttc
    14161 atcgtgttta gttcagtact tgatttaaat gtgctaatga tttttcacga ccaagcaatt
    14221 cgatcgtttc aggcaattct ggaccatgca tttgtcctga tacagctaca cggattggca
    14281 tgaaaagatt ttttcctttt acgccagttt ctttttgaac agctttgatg gcagctttga
    14341 cagttggagc atctactact tccatttgtt ctagctgtgc tttgaatgcg tggagaacag
    14401 taggaactgt ttcaccagca agtacttctt ttgctgcttc attcaataca ggatgttcat
    14461 tgaaaaatag cgtagacaga tcaacgattt ctgcagcata gctcatttga ggttgataca
    14521 ggctaacgat cttcttcacc cattcgattg tcttttcatc tgggttttct gctacacgtc
    14581 catcagcgat tagataaggc agacacatgt cagtaaatgc atctagatcc attgctttga
    14641 tgtaatggtt gtttacccat tgcagttttt tcgcatcgaa agctgcgggt gatttactta
    14701 ggcgttttgg atcgaacatt ttgatcaatt cttggtgttc gaagatttcg tcttcgccca
    14761 caggtgacca gcccaataat gcaataaagt tgaacattgc atctggtaga tagcctaatt
    14821 cgcgatattg ttcgataaat tgcaagattg attcgtcacg tttgcttagt tttttacctg
    14881 tttcactgtt gatgatcaat gtcatatgtc cgaaggttgg tggtgtccag ccaaatgctt
    14941 catagatcat cagttgtttt ggtgtattgg caatatggtc atctccacgt aaaacgtgag
    15001 tgattttcat caaatgatca tcgaccgcaa cagcaaaatt ataagttggc ataccatcgc
    15061 gtttttggat gacaaagtcg ccaccgatat tatcggattc gaacgaaata gcgcctttca
    15121 ccatatctgt aaatgcgtat tctgtattgc gaggcacacg gaaacgtaca acagattcta
    15181 gaccttgtgc ttctttttct gcttgttctt caggtgtcaa gttcgcacat gttcctgcgt
    15241 aatgaggcat ttctcctctt gcgcgttgtg cttcccgttc tgcttctaat tcttcttctg
    15301 tgcaatagca tttgtatgct cgattgctag caagcagctg atcaatcagc ggttgataga
    15361 tttctttacg ttcagattga cggtaagggc cgtattctcc aggattttca ggagattcat
    15421 cccaattcat tcccaaccaa gcaaggtttt ccaactggct tttttcgcca tcttcgatat
    15481 tacgtttttg gtcagtatcc tcaatacgaa taataaagtc tccatcattg tggcgggcaa
    15541 acaaataatt gaataatgca gttcttgcat ttccaatatg caggtgccca gttggacttg
    15601 gtgcatagcg tacgcgtact ttcgtcattt tcttcctctt ctttcaaact agactttgac
    15661 taacagcaaa aattccgtca gttacggctt ctcaaatatc atttatttaa agccactcag
    15721 cacaaatttt accttttcta aggtaaatag taaagccaac tggttgaatt tccttaccag
    15781 ttggctgaat ttctttttta ttttgattgt ttagtctttt tctctgtctc ttttttattg
    15841 tcgattcctc gtcctgcatg tgcaggttta gcaaagatca tgcgtccagc agctgtttga
    15901 agggcacttg ttacaacgac ttcgatacgt tcattcatgt aatgctgtcc atcttctacg
    15961 acaaccatcg tgccgtcatc aaggtaggct actccttgtt ggcgttctgt cccggctttt
    16021 actactagaa cattcatcgt ttcaccaggg atgacgactg gttttacggc atttgccaac
    16081 gcattgatat taagaacagg tacattttgg aattcagaga ctttattcaa gttatagtca
    16141 ttggttacaa tgacaccatc taaaagctta gccaatttga tcaatttgct gtctacttca
    16201 ctgatatctt caaagtctcc atcgtacatt tcgacagata taccctcttc tttttgcaag
    16261 gcatttagga tatctaatcc gcgacgacca cgcacacgtt tcaaactatc ccctgagtcc
    16321 gcgatgtatt gtagttcgta aagaacaaaa ttagggatca tcaaggttcc ttctaagaat
    16381 ccagtctttg taatatcata aatccgaccg tcaatgatca cgcttgtatc caagatttta
    16441 tatttatgga aattttctcc tgattttcgt tctaacagtt gactctcaac ttgactagct
    16501 gtttcttgct cgattttttt gcttcgtgaa ccaaaaactt tcttccattc ttcgattcgt
    16561 gtcgttccca ttctcaatcc cagatagcct aagatgatca tcagtaaaat cggcaataca
    16621 ctattgacga atggaatttc catattgtaa agaggggtcg agatcacaac acctaaagtc
    16681 aatccgatga tcgcaccgat cgcaccgaag agaagataag tcaagctgac ctcacttagt
    16741 ttttgttcta cttgtttgat aatagctgag atatgtttgg ccaacagcaa agataataaa
    16801 aagaaaataa gtgcgcctaa caggctgttg gtaaatgtat tattcagcca tccgtttgac
    16861 tgttgaccga ttgcctgcca agctgagggt aaaaatgaaa tacccaagct tgcgccgaca
    16921 atgatcatca gcaaagtaat gacacgtttt tgcatcgttt ttcctccgtt tcgacaatca
    16981 tttattagtt agcggaacac acgcttcaac gtttcagcca aagttgctac tccaactatc
    17041 tcgattcctt ttggcgcttc ccagttacct aagttgtttt taggtaggta tacttttgtg
    17101 aagccgagtt tttggacttc tttgactcgt tgttcaatat gactgacacg tctgatttct
    17161 ccagtcagtc caatttcacc gatgaaacat tccgttggct gtgttccttt ttctttatag
    17221 cttgaagcaa tactgacagc gatagctaaa tcaatcgctg gttcatttaa ttttacccca
    17281 cctgcagctt ttaagtaagc atcttgattc tgaagaagca acccagctct tttttccagc
    17341 actgccatga tcagagatac ccgattgaaa tcaagacctg ttgttgttcg tttggcattt
    17401 ccgaacatag taggtgtgac taatgcttgg atctctacca aaattggacg tgtcccttcc
    17461 attgccacaa cgatagcaga gccagttgca ccgtctaggc gttcttctaa gaaaatctgt
    17521 gaaggattag cgacttcttc caatccatgc tcatgcattt caaaaatccc aatctcattc
    17581 gttgagccaa atcggttttt tactgctcgc aaaatccgaa aactatgatg ttgttcacct
    17641 tcaaaatata acaccgtatc caccatatgt tctaacattc ttgggccggc gatcgagcct
    17701 tctttggtaa catgaccgac gataaaaatg gctatcccat ttgttttagc ggttttgagt
    17761 agttctgcag tggtttctct tacttggctg acacttcctg ccacacttgt gatgtctggc
    17821 tgcgtcatcg tttggatgga atcaatgata acataatcag gggagatatg ttcgatggca
    17881 cgactgattt cgttcatatc tgtttcagcg tatagataaa attctgtgtt gatacttccg
    17941 aggcgttccg cgcgtaattt tatctgctct gcgctttctt cgccggatac atataagact
    18001 tttccgccta tggctgctaa ttgctgagaa acttgtaaca gcaaagtgga tttcccgata
    18061 cctggatcgc caccaatcaa gaccatagac cctggcacca cacctccgcc taacacacgg
    18121 ttcagttctt ctaattttgt tttgatccga ggttcttttt tagggacgac gtctgcgatt
    18181 tttgttggtt ttgctttttc tcctgtcaaa cttgtccgag ttctgcggtc cgttgtatct
    18241 tgttcgattt cttcgaccat cgtattccat tttccgcaat ttgggcagcg tcccaagaac
    18301 ttaggcgaca catacccgca attttggcaa acaaattgta cttttgattt ttttgccatt
    18361 gtaccgcctc cttttatcct aatcatgaga cattaaatca agcttaagta tctttaacgt
    18421 tttatttact ggaagaacca aatccgcctt gacgttgagc agtcgtttga tcttgatctg
    18481 cttttaaaaa cggcaaaaag atcccttggc cgatacgctc gccttttttg atcactacat
    18541 ccttgaaacc aaagttggtg aattggaaca tgatatggcc ttcatttgcc tcattattgt
    18601 aataatcact gtcaatcaca cctactccgt ttgtcaacac taaaaaccgc ttcaacggat
    18661 tgcttgaacg gttagcaagc tgcaaaaatt catcttctcc catgtaagct ttgattccag
    18721 taggcacgag ggttggtttc aattgttttt cattttctga tacatctttt ttcaatccat
    18781 ctgcgactgt ttccaacccc tttttccagc ctgctttcca gatacttgga atcacgacct
    18841 cttctgctgc ttcaaaatca tagccagcag catgatgtgt agcgcgctgc ggtagattga
    18901 tattttgatc tttatattta gaaataactt caaatcctct ttttctcacc ggttttcctc
    18961 cctaaataaa ctactcctat tctaacatat ccgaggctgt aaaagacttt ctttacaaat
    19021 tcttcaagaa attctgctat cttaaagtaa accattaatt cgttcttttt ccaaacgact
    19081 gcaagttgta caaaaaaact attttttaca tgactttact cgtcaaaaca agtagaatag
    19141 aacgaaatta atgagtggat atttgattga ggtgtcacat gcgtaaagaa attaaaaatg
    19201 caaaacgaat tattgtcaaa gtaggaacaa gtacattgat ttatcccaat ggaaatatca
    19261 atcttgcggc aatcgatcag ttagcttttg tcctttctga tcttagaaat cggggaaagg
    19321 aagtgatctt agtctcatca ggagctgtag gcgttggtat gcataaatta catttgtcag
    19381 aacgtccgac tttgattcct gaacagcaag ctgcggcggc tgttggtcaa gccgaactga
    19441 tgaatatcta taatcaaaga ttcagtgtat ataatcaaca gacggcacag atcctcgtga
    19501 caagagacgt ggtagattat cctgaaagtc gtaaaaatgt gaggaataca ttggaacagc
    19561 ttttgattat ggacacgatc cctatcgtga acgaaaacga tactgtagca gtggatgaat
    19621 tagatcatct gacaaaattt ggcgataatg atcagctgtc agccatcatg gcaaaacttg
    19681 tcgcggcaga ccttttgatc gtcctttctg atatcgacgg gttttattca gataatccga
    19741 ctacaaatcc cgaggctcag atgtatcgct atatcgataa aatcgatgaa aagattctgg
    19801 cacaagcagg tggtgctggt agcacatttg gaacaggcgg tatgcagagt aaattaaaag
    19861 ccgcttctag gatatttgaa gcaaacagcg caatgatttt agcaaatgga aaaaatccgg
    19921 caattatttt tgatattctt gcaggaaaag aagtaggtac tttatttaag gaggacttga
    19981 aatgactgac ttgatgcaat taggacaaca ggcaaaagat agcgcgcaac aactagcact
    20041 tatgcctaca aaccgcaaaa atgagctact tgttcaaatg gcacaaacca tcaaaaagaa
    20101 tcaacaagcg atcattgaag caaatcataa agacttagaa aaagcagtcg aaaacaatat
    20161 cagtgaaacg atgcaagacc gcttgcagct gacacctgag cgaatcaatg ccatggctac
    20221 agagatcgaa aaaattgctt cgttagacga tcctattgga aaagtagatg agatgtggac
    20281 gaacaccgac ggattgagaa tcggcaaaaa gcgcgtgcca ctaggcgtga tcggcatcat
    20341 ttacgaatct cgtccaaatg tcacgacaga tgcagctagt ctagcattca aatcagggaa
    20401 tgccgttatc cttcgcggtg gcaaagaagc attcttctcg aatcaattat tagtccaatt
    20461 attgcagcaa gtactggtca gtgctggcga atctccttat gccatccagt ttgtcgatga
    20521 tacttctcat gaaactgcag gacagttgat gcgattgaca gaatacttag atgtgctgat
    20581 tcctcgaggc ggagcaaaat taatccaacg agtcaaagaa caggcaacgg tacctatcat
    20641 tgaaacaggt acaggaaata atcacgtata tatcgataaa gaagcacagt tatctatggc
    20701 agtgaacatc atcgtcaatg caaaagcttc ccgtccatct gtctgtaacg cggcagaaac
    20761 attgctgatc cattctgaga tcgcaccttt tttcttgcca gctattgaaa aagaactggt
    20821 agaacacggg gtctctttac gtgcggatgc tcgtgcgtta gaatatctag agacggctgc
    20881 tttagcagaa gacacagatt gggatactga atatctagat tatatccttg ctgtcaaagt
    20941 ggtcgattct ttggatgaag caattgcaca tatcaatcgg cataatacta aacattcaga
    21001 aacgatcgtg acagatagtt atgcggctag tcaacgtttc ttaaatgaag tggacgcggc
    21061 ggctgtctat gtcaatgcct ccactcgttt tacagacgga tcggtatttg gttttggtgc
    21121 agagattggt atctctactc aaaagttgca tgcccgtggt ccaatggggt taaatgaatt
    21181 aacttccaca aaatatatca tttatggcga tggacaaatc cgcgaataat gtttcgtgtg
    21241 aaacattatt cccgcctttt gccttttttc tgtaaaggtt ctatccaact agaaaggtgt
    21301 caatatgttt gaaattttaa tgattttttt tatttacctt atttctctga atatcgctgc
    21361 ttttctggga gtcggtattt tatccctttt ctttcaattc aaaaaacgaa gcatagggag
    21421 tcaacgggaa aaatggtcgc agtattttga caaaatcggg caaaaagggc tggttacacg
    21481 cttgcacatt agttacatgg ttgcattatg cttattagcg acaatcaatt actattcttt
    21541 tttcgatcat tcaatcgctt atacgattac gctactgatt gctgggattt ttcacttatc
    21601 ttataaatat cagctgaata aaaaccactt gaatcggact ttccgctaaa aaatatccgt
    21661 acgataagta gagtgccttg aagatcggca aaaaactgcc ggacttttta gaggctgtga
    21721 caaagatttt gtcacagcct ctttttccat ataaacggcg ttcgcctacc agctgcctgg
    21781 taaatctgtt tcctcttcgt taagaagcga aaatccgtca aatgtttcgc catcataata
    21841 acgaagcagc tcatccatgg acaagttcat cgttttttct ttttcttctt ccgctgattc
    21901 agccagtagc tgttcccgat acacctgata cataccagct attttttgct gcatcgcttt
    21961 tctagtttca ccttccgcat tgattattgg caattctttg atatagccac gataaatcaa
    22021 ccgatcatct gaaaattcaa tgggtaattc aacgagtgtc aacccgactt cctgaaaata
    22081 ttcttccagc atctattatc cccccattct ttttcttaaa tgaattttag cttacctact
    22141 gaaaattttc cagtttcatt attttgagaa aaaaagggaa tgtgacaaaa atcgtgtcac
    22201 atcccctttt tccgaataaa tggtgttctt ctgccaactc ctcaatcata agtcacaata
    22261 ttcaacaaac atgagaaact gttttttgaa attgttcctt gtgattcaga gttaaacgac
    22321 agtctcacaa cctcttggta aacggcgttc aatcagctga ccctcggtat tagctcaaaa
    22381 aacggtaaaa tatgagaagc tatttttccg ttttgtttct ctaatactta ggataaaacg
    22441 ctgattttac aacctctgtt aaacggtgtt caatcagctg tccttcggta ttagctcaaa
    22501 aaacggcaaa atatgaggaa ctatttttcc gtttgtttct ctaatactca ggacaaaacg
    22561 ctgattttac aacctcctga taaacggtgt tcaaaagctt gcatctgcgg taataagccc
    22621 aaccaaacga aaaatatgag aagctatttt tgtttggctg ttcttatgac ttgatgcagg
    22681 acagcttttt cacaacctct gatttcttat tcttcttcat ttgtagcaaa ataattacgg
    22741 tgataaatga tacaacatac actgataaga acaatggcac ctgcaaaata aaacgcatag
    22801 ctttcaccaa tatgttcacc aatcacacct gcaataaatg gtgctaaagc tccaccgaaa
    22861 aagcggacaa aactatagct ggacgaagca acgtttcgtt gcagacctgg tatttccata
    22921 gcaatcgtcg tcaacagtgt attgataatt ccttggaaaa atcctgctaa cacaatgctg
    22981 atcggtacaa ggactggcat agaagttcca gctcctaata acagcaaaca aataatgaat
    23041 aagacaaatg ctgctaaaat tgtccgataa gtcgtaaact tccgctcaag agccggtgca
    23101 acgaaaatcg aagcaatagc cagaagcaca ccccagccaa aaaatacaaa accgacttct
    23161 aattcagaat agccaatcaa taaaaatggc gcatacgcaa gtaaagtaaa gaatccgaaa
    23221 ttatataaca atgcgattag tccaactgaa cgaagtttat gattgcttaa cgctttgaca
    23281 ccttcccaaa ggcctacttt tctctttggt ttctcagttt caggtaaaag taccgctagg
    23341 gctataaaac cgcaaaacat caaaaatgct accccaaaaa atggataacg ccatgaacca
    23401 ctgcccaaga ccccaccgac tagagggcca acagccattc cgcagccaag tgctgcttca
    23461 tacatcatga ttgctttttc tgtgttacca actaaaatac tgacgatcgt tgctaaagca
    23521 gtagagacaa ataaagcatt cccgacaccc cagccggcac gtaacccaat cagcattccg
    23581 atattcccag ataatccgcc aagaccagcg aatacaacga tgataaacaa tcctgtcatc
    23641 aatgtttttt tcaaaccgat ccgactagat aagaaacctg taaatagcat caccacacca
    23701 gtgactaaca tgtaactagt aaataacagg gtcgtttcag ctggcgtagc gtttaactgt
    23761 gcagcaatcg tttttaagat tgggtctact agacccactc ccataaaagc gatcatacag
    23821 gtagctgcta caactagcgc tgaacttttc ccctttgttt cttcatttgt ttgttcttgt
    23881 atatctattt gattctccaa tatttccatc cctttctctc tattagcatc atacatgatt
    23941 tattttacat gtcaatatgc acatgtaaaa cgtggaaact ttactgtgat ataataaaga
    24001 aaataagacg agaggtagga tttttatgaa tactttagcc tatatattat tatgcgtgct
    24061 tttatccaaa ccacgttctg ggtatgaatt gaagcagctg atcacaattt tttgggaagc
    24121 ccatcacagt cagatctata caacgcttgc caagttagac cagcagggtt atgtgaccgt
    24181 tttggacaaa gacgagcact ctcaaaaaaa gatctaccac ttgactgatg aaggacgtgc
    24241 tcttgtagag gaatggatca aagaggaaac tcccgctcct acgcaaaaag atgaatttct
    24301 agcaaaaatc tatgcttttt cgacgctcga taaaacaaca gctactggac tgctattcac
    24361 tagagaacaa cagctgaaca aaatcatgca aaaaaatcaa aaaaaattga atgacctctc
    24421 gctagacaag aaacaagaat ttggacgtta tgttgtgatc cagcgcagaa ttatgctttg
    24481 tcagcaggaa ctccaatggt gtcagcaagt aagagaacaa atgcagcagt tttttgagta
    24541 agggataaac agaacctttc tagaaatatc tattgaagag caaattatct tcaataaaag
    24601 agcaatacag ttggaaaaga aaaagcgatc cgaacgacag gccaaaacct atcgttcgga
    24661 tcactatttt tctagaactg cattcaagag actatgacaa aaagcatgcc aaggcctttt
    24721 ttcaaataac agtgttccat cattcattac tttgaattgc ttttagcaca cttcttgaaa
    24781 gcgggccaac gatgatcaac tgcaacggta aagcggcaat cacattcatt ttccaagcat
    24841 tcatataagc agagaataat tgatcaggta ttccttgttc catgatcaca ccgaataaag
    24901 acatacatgt caccattcct aaaaccatca agcatgaaat agttaagatc atttggacct
    24961 tgctttctgg atttactggt agtttaaagg caattttttt cgccagcaca ccgatgatga
    25021 agacatccaa aatgaaagcc accacgaatc caggcactag accaatcaac aaacttgata
    25081 aatgaagatc ttgatgcagc cataaattat acgcgctcat ccccaagacc atcaaaaaac
    25141 acatcattgt tgtaaaaatt attccttctt ttttatttgt aggcaatctg taccactcct
    25201 ttaaatttgt cacgaaaatc atcgtagcac atttaaaaat ttctcgtaag tacttttttt
    25261 attttctgcc cttttatttc ataaacttca tcagccgtac gctcgacaaa gtaagaatca
    25321 tgagaagtaa acaaaatcgt tcccggatat atttgcatta gcttctctaa agcttcgatc
    25381 gttgccatat caataaaatt agtcggttca tctaacagca acacattact tggcttagta
    25441 aaaagcaaag caatcgtcag acgggtcgct tctcccccac ttaatgtaca taacggtttc
    25501 gtcaaagttt cctgagcaaa ccctaagtga ttcagcaaac tacggacaag cgattctgaa
    25561 aactctgttt gtctcatgac aaaacgaatg atggattctt cagatgtcat ttgataaccc
    25621 ttttgctgat atacttgaaa gcttactttc ggagagagaa ggattccttc tccttgtttg
    25681 cggatatgtt ctaataaggt tgtcttacct gaaccatttt caccgacaat cgcgattttt
    25741 ttacctaaag aaaactgaaa atcaccattt accagtaatg ttctcccact tctttccaat
    25801 tggacatttt gtcccatgat gggatagcgg ctgtggagtt caagagattt tggtactgga
    25861 aaacggattt gtttcatctg ctgcggcttg gttgtttctc ctattttttg gagtcttctc
    25921 tctaatgttt tcgcattctt ttggatggct ttttgtacac tgtctttttg tttagaggaa
    25981 gacaaccgat ccagtctgat ttgttgcttt ttctttttac tggacacttt ttccaacttt
    26041 tccgcttgtt ttttcttttc ttggatcgat ttttccaatc gttttttctc tttcatgatc
    26101 gactctgctt ctcgtagctg agtctttttc tccaattcct tttgagtgag ataggcacta
    26161 taattccccg aaaactctcg gatttctccg tcttttactt cccagatttt cgatgctagc
    26221 tcatctagaa aatagcgatc atgactaaca aaaaccagcg taccataata ataacgaagc
    26281 tcttcaatca gtcgatcaat actttttcta tctagatggg ttgttggctc gtctagaagc
    26341 atcccttgtt cataagaaga aagaagacgt atcaactcga tctttcgttc ttcccctccg
    26401 ctccatttcc gctctgaagt ttcttccagt cttaactgga aatgactggc taattccaaa
    26461 tttaggtcct ttgcttcagc gagataggtt aattgaggca ggtagttgaa ggtgatctct
    26521 gtttggattt tcccatgatc gggaaaaagt tcaccggcta ttagtttgag caaagtcgat
    26581 ttaccgacac cattttctcc tatgatccct atacgatctt gctgatagac acgaagagaa
    26641 tcaatagata gttctagctg gttgccaaag ctgacttgta gatttgttat atttactgct
    26701 aaattttcca tatgttttac actcctattt catgtaaaac aacaattatc gatactaaaa
    26761 aagagcagaa aaatctgttt gcaaaaaaac tgcatcacat cttttcctac tctgaacgaa
    26821 ataactatga ctaaatcacc aaatctgtca tagctttcac ctattctact gtcgttcgaa
    26881 aaaatggaca tactccatcc atgaaattgt tgtttataca tgtcttttga atttatttag
    26941 ttgttattca aaagttcgaa acgtaatttc atcgatgcag tcatttttcc gacctccttt
    27001 tttattaggt taccacacac gattcaaaaa aagcaatagc tactgaatag taactgaaaa
    27061 ccttttattc gaacatagtg atcttttctg gtgctaaacg aacagactcg taaccttctt
    27121 ctaccgcttt tcctattgat aaaataataa ctggaacata acggtcttta tccaaaccaa
    27181 atgcctctgc caactgatct gcttcgaatc caccaattgg atttgtttca tagccatgat
    27241 cacgcgcaac taacatgaat tgcatagccg ctaagctaga atcgactttg actacatcgt
    27301 tcatttcttc tcttgaaaaa ctcttgtaat aaggaatgat cgcagctaat tgttgatctc
    27361 tgacttcttt tggcatcttc cctgattcgt aagcttggtt gtagatttct tcgccatatt
    27421 cgtaacattg catatcgcca aaaatcatga ccattgcaga agaagtatca ttttgtctcg
    27481 tattgaaacg gatcaaagga cgcaagattt cttttgcttc ctcgctttca gcgaccacaa
    27541 agcgccatgg tttcatattg acagaagaag gtgcagtcgt cgcttctttg atcatttcca
    27601 gcatttcttc gtgtgggatt ttgtatgttt catcatatac gcgaacggat cttctaccag
    27661 tcataattgt tgaaaaatca tttttttgtt ccattattat taacctcttt cgtttctgtt
    27721 gttatattgc aaagttgggt attcaattta tctaaaagtg ttagaaattg tttcatttct
    27781 acatcgctga aagccgggga tagcacggaa cctgtatctt tatggtccat ttcacaatgc
    27841 ttgattttct tctgggcttt ttctgttagc gctacataca cttcccggtt gttctgagga
    27901 tttctctgac gcgaaacata gccatttgct tccaatacct tcagatgacg agtaactgct
    27961 ccttgatcaa tctgcaactt ttcttgcaaa ctgctttgta gcaatggatt ttcttctttc
    28021 agtctcatca tgatctcgta tctagtaaga cttataccga tattttcttc aaagatctgg
    28081 acgatttttt gatcggctaa tttgatttga tataaaaggt gactaatctc ttctaaagac
    28141 ataatttcct cctttctaat aattgactcg tcaacgattg acttgtcaat aatagaataa
    28201 aaaaagaaaa aagtcaaaga atcagctgtg taataaaaaa aacgctctca atagaaaagt
    28261 atagagaaaa gaaatttttt gtagaatgaa cggaaaaaat gataaagaaa gcacaacgct
    28321 aaaaccaaga ttattgattc ttatcaaact atagaagata gctcttctgc agtagataat
    28381 tttacaccgc caaaaatttc cgaaatcgcc ttttgcaaga atcccgtcga atattagaaa
    28441 ttggatcagt atgcttaaac agtaataaaa aagttatgac aagatgttgt cacaaccttt
    28501 tttgagtaaa cggtgttcaa aaacttgcat ctgcggtaat aagttcaacc aaacaaaaaa
    28561 catgaaaatc tgtttttgtt tggctgttct tactacttga tgcagggcag ctttttcact
    28621 acctcttttt aataaccata aaatcctatt ccttcatatc tccagcccat cggtgttagc
    28681 caatctcttt ccttagcact caaggtatag tgatgggtac cagttttttc atttggatta
    28741 aatagtcgat aaattggctt ggaaccacca gaataaaaac ttagaccttc ataacgccaa
    28801 ccgatttttg ttaaagagtt gatctcagat tgtgccattg tgtaatgatg gtctccactg
    28861 taaggattgt ataaacggta gacaggttgt ccgctgtttg gtgcttgcca agcaatccct
    28921 tcatattccc agcctacagt tggcagataa tttttttctc cggcattcaa tgtatataaa
    28981 tgttctcctg tatgacggtt atacagacga tacatcggag aagttgaagt tggtgctgta
    29041 tcatactgtg tcagattata ggtttcgatt acattgttca atgtagaacc ataattaggc
    29101 gcagtggcat aacgacctgt tagccaagcc gtagcatctt tgtaagagtt cgtattgctt
    29161 ttccatgcac cggaatagta gtaaacgcct ggctgaaaag aagtcgtctt caataccgtt
    29221 acgttatctt gcaatgattc tttgtacgaa ggatattttc taaaagcggc attcaccgta
    29281 atccattgcc cattcacata ttccgaagta ggcatcgtaa cagattggcc attatagctt
    29341 cctttaatcc caaatagatt gtaattaggg gcagaagcca aagtactgtt cccccagcca
    29401 ctttcaacga tggcttgagc aatcatgact gatgcatata agtcattggc acttgccaaa
    29461 ctttgtgcag ttgaggcaat actatcaatg aacgcttgtt gagaattagc agatgagcga
    29521 gcataaaaag catagccagt atagttttcg aattcagaca aatctgcttg atgggtatgt
    29581 gtcaatgttt gcccatctgt caattggttc gtgatttctt ctgcattctc tggattgttc
    29641 tgagcttctg ctgatgtact gtcttcggtt tgtgtttgtt ctgttgtttc agttgttggt
    29701 tcttcggttg aaattgtatt ttctgagaca gtagctgact gcaaagtact ttctgtatcg
    29761 ttttcaattc ctgtttctga agaactcgat gtctgagttg cgacagtttc tgtcgattct
    29821 tcagctagca ctgttgtgtt ggcaactgct agatttccta aaagaataac gctaaataat
    29881 aaattctttg ctttcatctt ttttctcctt tgaattatga aacttttcct aaataacagt
    29941 gtaactaaaa aagtaaaagc aagtcaatgt ccatatttaa ataacatttg tttcttttta
    30001 ataaatttgc tttttctgca taaaaagacg taaatgaaga atattcttct ccattcacgc
    30061 cttaaattga tggtcgtttg aataactatt taacaagtaa ttctctctgg atattatccg
    30121 ttatggatta tacttcctgt gtcgtcggac ggataatcat gttgctgaca gacatccggt
    30181 ctggcgtttc tatcgcaaat gctacagcag aagctatatc ttcagatgtt aatccccatt
    30241 ctttttgcgc ttcatgaagt tcaagagcag cctttttgtc gctgatggtt tgatataatt
    30301 cagtttgaac agctcctgga gagatgatcg tagatttgat gttattttgc cgttgctctt
    30361 gacgtaatcc ttccatgatt gcacggacag caaattttgt tccgcaataa acggcggaat
    30421 caggataaac aacatgtccc gccacagaat cagttgaaat gatatgccca gatttttgtt
    30481 tttccatgat tggaagtacg gcggaaattc catttaacac acccatgatg ttgatatcaa
    30541 gcatattttg ccattcatca cggtgacctt caactaatgg agcggttggc atgattcctg
    30601 cattattgta caatacatcg attcgaccga acttttccat cgctaaatcg ataactgctt
    30661 ggacttgtgc aaaatctctt acatctgctg tttgaatata tagctcagct tctggtaaag
    30721 attcttttat tgcttttagg cgatcttctc ggcgtgcagc tataactagt ttagcccctt
    30781 tttctgctaa tagcttcgtt gttgcttcac cgattccgct tgaagcgccc atgatgacaa
    30841 tcactttttc tgataaatca ttcattctat tctcctcctg ttttgaaaaa tctcttgctt
    30901 atactcacta tctcttattt catcttactg ttcaaataat ccgttcgaca ggtttgacga
    30961 ttttggcagg gattcctgca acgatggtat tgtctgggac atctttcgta acagttgcat
    31021 tcgctgcaac aatggcattc tcgccaatcg ttacgcctgg cagaatagtt gctcctgctc
    31081 cgatccaagc atttttcttg atgtggattg gctttactga caagcctctt cgttcttttg
    31141 gatcggttag atggttcacc gtgatgaggt tgacacgagg accaatcaat actgaatctt
    31201 ctaaacagat gccacctaag tcaacgaaca tcacttgacg attgataaag acatttttcc
    31261 ctatactaat atgtttgccg aaatctgtat aaaaaggcag cagaacagtc accgtatcat
    31321 ctactttgtc actaatgata ttttctagcc gtttgcggat atctgctggt tcatggtacc
    31381 cagtattcag cgctgctaaa ggctcttcgt tttccgcttg gatctgatgg atcacttcaa
    31441 agagcggatc cgttactcga atctctttat tgacgatttg tttttcgatg aggcttgtca
    31501 ttttgctccc tccttttatc cttactttat aacggacggc ttatgatgtc taatacttat
    31561 aaaacatact taactattcc ttttaactat acttagtggt atgctttttt aaaaacagga
    31621 gggaaaaaat ggatattcgt atactgacgt attttttagc agtagctaga gaaaaaaata
    31681 tcagccgagc agcaaaatct ctgcatatca cgcaacctac tctctcaaaa caactgaaag
    31741 atttagaaga agaacttggt gtgacacttt tcactcgagg ctcccgagag atacagttga
    31801 cggattctgg tcgatatctt tattcaaaag gaaaagaaat cttgtcgttg gtagaaaaaa
    31861 cggcgaacaa tttaacaaat gacgcaatca tctctggaga aatctacatt ggcggaggcg
    31921 aaactaaagc aatctctttc atttctgata gccttcattc gttgatcaag gcgcatcctg
    31981 atatccagtt tcatttatat agcgggaatg cagatgatat catagacaaa ctggatagcg
    32041 gattacttga ttttggactc gtaatcgaac cgacaaacaa acaaaaatac cagtcatttc
    32101 gactgccagc ccaagatcgt tggggacttt tgatacatcg gaatcatcct ttagcaaagc
    32161 aaacagtcat ccatccgcag gaactgaaaa ctgttccttt gatcatttcc cggcaaacgt
    32221 cagtggacag tcaactagct gaatggctag gtttcagcat agatcagtta tcgatcgtcg
    32281 gaacatataa cttattgtac aatgcttctt tgatggtcga taacgggact tttggtgcat
    32341 tagctctaga tgggatcatc aatacttatg ggacggaaaa agtattcatc ccactcgatc
    32401 cgccattaca agcttcactg aatctcattt ggaaaaaaga ccagcctctt tctcctgctg
    32461 ctcgtaaatt tttagatgtc ctaacggaga aatacagtta agtagagttt ttttccttcg
    32521 ctttcagctt tttaacaacg aataagttaa gcaaaagatc caatgattaa ataacataag
    32581 tttcttaatt tgttccttcc ttttat
//
LOCUS       DAZKLA010000021        32179 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000021 DAZKLA010000000
VERSION     DAZKLA010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.021"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(120..500)
                     /gene="yajC"
                     /locus_tag="ACG85N_000800"
     CDS             complement(120..500)
                     /gene="yajC"
                     /locus_tag="ACG85N_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFQ0223596.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(597..1742)
                     /gene="tgt"
                     /locus_tag="ACG85N_000801"
     CDS             complement(597..1742)
                     /gene="tgt"
                     /locus_tag="ACG85N_000801"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFQ0223597.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1960..2658
                     /locus_tag="ACG85N_000802"
     CDS             1960..2658
                     /locus_tag="ACG85N_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0223598.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2826..5465)
                     /locus_tag="ACG85N_000803"
     CDS             complement(2826..5465)
                     /locus_tag="ACG85N_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0223599.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6084..7115)
                     /gene="queA"
                     /locus_tag="ACG85N_000804"
     CDS             complement(6084..7115)
                     /gene="queA"
                     /locus_tag="ACG85N_000804"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFQ0223600.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7308..8482
                     /locus_tag="ACG85N_000805"
                     /pseudo
     CDS             7308..8482
                     /locus_tag="ACG85N_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
     gene            8486..9127
                     /locus_tag="ACG85N_000806"
     CDS             8486..9127
                     /locus_tag="ACG85N_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0223601.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9124..10041
                     /locus_tag="ACG85N_000807"
     CDS             9124..10041
                     /locus_tag="ACG85N_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0223602.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10038..10709
                     /locus_tag="ACG85N_000808"
     CDS             10038..10709
                     /locus_tag="ACG85N_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0223603.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11250..11738)
                     /locus_tag="ACG85N_000809"
     CDS             complement(11250..11738)
                     /locus_tag="ACG85N_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFQ0223604.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11939..12205)
                     /locus_tag="ACG85N_000810"
     CDS             complement(11939..12205)
                     /locus_tag="ACG85N_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0223605.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12205..12468)
                     /locus_tag="ACG85N_000811"
     CDS             complement(12205..12468)
                     /locus_tag="ACG85N_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0223606.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12989..13129
                     /locus_tag="ACG85N_000812"
                     /pseudo
     CDS             12989..13129
                     /locus_tag="ACG85N_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(13271..15094)
                     /locus_tag="ACG85N_000813"
     CDS             complement(13271..15094)
                     /locus_tag="ACG85N_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0223607.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15692..18397)
                     /locus_tag="ACG85N_000814"
     CDS             complement(15692..18397)
                     /locus_tag="ACG85N_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFQ0223608.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18703..19650)
                     /locus_tag="ACG85N_000815"
     CDS             complement(18703..19650)
                     /locus_tag="ACG85N_000815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFQ0223609.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19840..20109
                     /gene="rpsN"
                     /locus_tag="ACG85N_000816"
     CDS             19840..20109
                     /gene="rpsN"
                     /locus_tag="ACG85N_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0223610.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20285..20647)
                     /locus_tag="ACG85N_000817"
     CDS             complement(20285..20647)
                     /locus_tag="ACG85N_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0223611.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20658..21779)
                     /gene="alr"
                     /locus_tag="ACG85N_000818"
     CDS             complement(20658..21779)
                     /gene="alr"
                     /locus_tag="ACG85N_000818"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFQ0223612.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21795..22145)
                     /gene="acpS"
                     /locus_tag="ACG85N_000819"
     CDS             complement(21795..22145)
                     /gene="acpS"
                     /locus_tag="ACG85N_000819"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFQ0223613.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22275..23786)
                     /locus_tag="ACG85N_000820"
     CDS             complement(22275..23786)
                     /locus_tag="ACG85N_000820"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0223614.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24108..25460)
                     /gene="murF"
                     /locus_tag="ACG85N_000821"
     CDS             complement(24108..25460)
                     /gene="murF"
                     /locus_tag="ACG85N_000821"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFQ0223615.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25613..26689)
                     /locus_tag="ACG85N_000822"
     CDS             complement(25613..26689)
                     /locus_tag="ACG85N_000822"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFQ0223616.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26885..28630
                     /locus_tag="ACG85N_000823"
     CDS             26885..28630
                     /locus_tag="ACG85N_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFQ0223617.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28910..29347)
                     /locus_tag="ACG85N_000824"
     CDS             complement(28910..29347)
                     /locus_tag="ACG85N_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFQ0223618.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29331..30704)
                     /locus_tag="ACG85N_000825"
     CDS             complement(29331..30704)
                     /locus_tag="ACG85N_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223619.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31026..31514)
                     /locus_tag="ACG85N_000826"
     CDS             complement(31026..31514)
                     /locus_tag="ACG85N_000826"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0223620.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31536..32138)
                     /locus_tag="ACG85N_000827"
     CDS             complement(31536..32138)
                     /locus_tag="ACG85N_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0223621.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9199 a         6882 c         5941 g        10157 t
ORIGIN      
        1 cacacccgat tattcaatat tgagcaatat atcccatgct cttatctctt tcttttttga
       61 ataaaaaatg aggtaggaca tctttctgcc ctacctcgtc tttaaataag ctttgatact
      121 tatttttctt ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt ttttacttca
      181 actgtctcag ctggttcttc tgttgtttca acagtagtcg ttgcatcatt tgttacgaca
      241 gtccctggtt tgaccgtttt gattgctgca cgatcgtact caaggaagat cccttcgcag
      301 tctaaaacaa ctgtccgttt gtcattgtcg atttctgaaa caactccatg taatccgccg
      361 atcgttacga ctgaatcacc tggtttcatg ctatctaaca agttttgacg ttcttgctgt
      421 tgtttctttt gcgaacgtga cataaaaaac cacattgcta atagcgcgat aaacatcaca
      481 agcattggta atgttcccat ttttttcacc tcactaattc tgtatctaca aaaaacactt
      541 tagcagaaaa atgctgataa cactagtttt ttacttaatt tttaaaagat tttcctttag
      601 aagctttttg cgttttcttt attgaacccg tattcttcaa aaaaggcttc tctaaattca
      661 agcaaattat catccatgat tgcttgacgt acttgtttca ttagattcaa caagaagtat
      721 aaattgtggt aagaagtcaa acgaatccca aatgtttcat cacatttgat caaatggcgg
      781 atataggcac gtgtatagtt tttacatgta taacaatcac atttctcatc aattggacgg
      841 aaatcatggg catatttcgc atttttcact actagacggc cttgagaagt catacatgtg
      901 ccatttcgag cgattcgagt aggcaagaca cagtcaaaca tgtctactcc acggataaca
      961 ccatcgatca atgaatcagc agcaccgaca cccatcaagt aacgtggttt gttttcgggg
     1021 atcaacggtg tagtgaattc taatacacga ttcatttctg atttaggttc cccgacagat
     1081 aacccgccga ttgagtaacc agggaaatcc atcccgacta gatctttcgc gctttgacga
     1141 cgaagatctt caaaccctgc tccttggatg attccaaata atccttgacg gtctggatta
     1201 gcatgagctt tcaatcctcg ttctgcccat cgagacgtac gttctactga ttttttcacg
     1261 tagtcgtaac tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat atcagagccc
     1321 aatttatttt ggatgttgat tgctttttct ggtgataaaa acatacgcga accgttcaag
     1381 tgatttttga aatgcacgcc ttcttcttcg atattccgca tatcacttaa tgaaaaaact
     1441 tgaaagccgc ctgaatctgt taaaatcggt tgatcccagt tcataaattt atgcagtccg
     1501 ccagcttctt ctactaagtc ttcaccaggg cgtagccata aatgatatgt attacttaaa
     1561 ataacgccag cacccatttc tttcaactct tctggggaca ttgtttttac tgtagctaat
     1621 gttcctactg gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat caattcccct
     1681 aaacgcgcgc cagtatgttt ttctttcttg attaaacgat agcgaatggc aggttgcgtc
     1741 attcatttcc ctactttcta tataaaatat cttgacaact cccaccatca taatcgtttc
     1801 aagtgctttt tgcaagttcc ctcggcttcc ttctacttta tttaatattc ttttttttat
     1861 ccttttttta tgaataacaa aaaaaagaaa aacattatga cagaaatatt ttttgataca
     1921 tggtatattg gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta
     1981 aaacgccaag caaaagagag tctaaaagga cgttggggac aagctgtttt actaaatcta
     2041 atccctacat tgattggtat tgctactatt cttcttatta tgattcctat tatcttgttt
     2101 gccaccacca tatatgatgg atcagcgagc gtctcagaag cgacaacttc aggaggcggt
     2161 agttcaggag gcggtggcgg aattatttcc agcatcatta gcgctttgtt catgagcggt
     2221 atttcttgga cttatcttga tattttacgt ggacaaaaag atcagatcga accattcaaa
     2281 gatgcatttc gtggattcaa aggtttgttt atcggcggag tgatcagctt agctgtgctg
     2341 atctcgatct ttacaacatt atggggtttg ctattagtta ttcctggaat cattaaaagc
     2401 tattcttatt ctcaaagcta ttttatttac tacgatatcg tccaagaaac aggacaaaca
     2461 cctaaagtac tggatacgat cactgcaagt cgaaaattga tggatggtta taaaggacaa
     2521 cttttctggt tagatctttc atttatcggc tggcatatct tagcattagc aactgctggt
     2581 attggttatc tatggctcag cccatacatt tctgcaacaa aagcagcatt ttatgaacaa
     2641 ttgccaaaag atatttaaaa gagagtgtga cttgtgaccg agggggtggc aggagaacac
     2701 cgtttatcga gaggcagtaa aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa
     2761 cattcatgtc acacctcctt tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca
     2821 gttaatcatt cctcttcttc tgcttccggt aatcgatgga acttgatcag ttcattcacc
     2881 gcgattggta cgatagataa aatccctacc aagaaccagt gattcaaact gatgtgtact
     2941 aggccgaata gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat agctaatgcc
     3001 agtaacgcca tttctaccaa agctttattg gtcgctaaat tgactttgaa gatcgattgg
     3061 ctgctgcgta cattgaacac atgcagaatc gatgaataag ctaagatcaa gaatgcaaca
     3121 gtttgaccga catgctgact tgcgtctacc catgggctga tagtatcgac ataactacct
     3181 aaataataac caaacaaggt tacgatggtg aagacccctg catttatggc aattttttgc
     3241 aataaacctc gagcaaagat cccttcattt ttcggaatgg gtggctgatc catgatgccg
     3301 tgttccgctg gctctcttcc taaagcaaat cctggaagtc catcagcaac tacattgaca
     3361 aacagtagtt gcacagctgt aaatggtgct ccccatccta acagcatcgc aatcaaaaca
     3421 ataaagatct ccgaaatatt acagcttagc aaaaagttga tagctttacg aatattctga
     3481 taaaccgaac gtccttgact gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa
     3541 atcatatctg cggcactttg cgccacatct gttcctgtga tccccatggc acacccaacg
     3601 ttacttgctt tcaacgctgg tgcatcattc acaccatccc cagtcatcgc tacgactgca
     3661 cccgtacgtt gccaagattt gacgatccgg atcttgtctt ctggcgttac ccgagcatag
     3721 acagagagag ctttgacacg cgcatctaat tcttcgtctg ataattgatg cagttctgat
     3781 cctgaaagtg cttctgacgg atcattcaag atacctaatt ctttcgctat ggcactggca
     3841 gtcaccacat gatctccagt gatcatgact gttttgatgc cagccttttt cgctcgcttg
     3901 attgctcctt tactttctgg acgtggtgga tcgatcattc cgatcagacc aatcaaacgt
     3961 aagtcttttt ccagtgcttc agaagaaata tcttgcggtt cttcgtcata agtagcataa
     4021 ccaacagcaa tcactctcag tgcccgttta ccaaagcgat cattaacgat cgccgcttgt
     4081 tctacatcgc ctgaactaaa gcgcggagca agaacatcga atgctccttt ggtgattgag
     4141 atgtattttt cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga
     4201 agctcgttta ctcgtggata tttttcttct aattctgcct ttgtatgata attctcttca
     4261 acagccctta cgatggctac ttctgttgga ttacctttga taacaaggtc gtcatcttct
     4321 tgctcgaccg tcacatctgt acatagtgca gccattttca atatttccat cgcgctgttc
     4381 gtcattgaat cttcgatatt cgttacttca tcttctttgc tccatacccg gcggacgcgc
     4441 atttcatttt gtgtcagcgt tcctgttttg tctgagcaga taacattagc ggttcctagc
     4501 gtttcaactg ctggcaactg tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg
     4561 tatgcaagtg tcaacgtaac aataacagtc aaggtttccg gtacagctgc aacagccaag
     4621 gaaatcgaag tcatgaacat ctcaaggagt ggttctcctt gcagttcacc aatcacaaat
     4681 actaaagccg cagccgctaa agcgatcata ctgattcgtt tgcctagctg attcaatcgg
     4741 acctgtaaag gtgttttttg catagtattt tcgttcaaaa gatccgcgat cttacccatt
     4801 tctgtagcca tgccaatggc agtgacgatc gctttcccac gacctgcagc aaccgtacat
     4861 cctttaaata ccatattaag ttggtcacct aatgactcat cttcttcagc aataaatgct
     4921 gcatcttttt caactgcttc actttctcca gtcaacgccg attcttctac acgtaattgt
     4981 gatgcttcga tcaaacgggc atcggcagaa atagcatcac cggactctaa aacaagaata
     5041 tcacccacta ccagttgctc tgactcaaca gtggtcactt tgccgtctcg tatgacagtt
     5101 gccgtttgtt tattcatatc ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg
     5161 acaatcgcca ataccgagtt gatcacaacg atagcgatga tcagcagtcc ttcaaataaa
     5221 tccccatgtt ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac aagaaggatg
     5281 atcgttgtaa aatcggacaa gcttcgaaag aattttttga tcatcgattc tttcggcgcc
     5341 tcatcgaatt tattcaatcc ctgctttttt cgccgttcat ccacttcttg tgaagacaac
     5401 ccagtatctg gatctgcttc tgttaccttc tttacttctt ctattgtctg cttgtattct
     5461 tccatcctat tcctccttta aaatcttcgt acaaaaaaag gtacttattt tactataaca
     5521 tccttttttt atttacttca aataaagggc ttatcgatta tttgttaaga aagcgtaaaa
     5581 tttaaagtgc ttatatttgc aaacgctacc aaaggatgct attatataga tgtaaggaag
     5641 gaggatacca tcaaatagac agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt
     5701 gttagaagta caggtttcac ccagacatgc gtgtagagta caaacctata aatagtgaat
     5761 gcgtatggat gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat
     5821 tgaataacgt aaacgtctga aaattgaata ggaacaagag atgtttcaaa tgttgaagat
     5881 gtaacgaaaa caaaattttt gtttaacatg aagaaattca acaccgtgct ttgcatgtat
     5941 gatggaaagt tacaatcatt tctgtttatt tgaatccttc cgaggtacaa gaaagccgag
     6001 cttatcgaga gctcggcttt ttctttgtca taaaatcttt gtaaagcttt tttgttattc
     6061 aaaactagcg gcttttcata accttatttc acgaacatcg catctccaaa actaaagaaa
     6121 cgatagcgct cttcaatcgc atgctggtaa gcagataaag tcaattctct tcctgcaaag
     6181 gcactaacta acatgactaa agtcgatttt ggcaaatgga aattagttga gaaagcttct
     6241 accactttga attcatagcc aggcgtaatg aaaatatcag tccatccgct gtctgctttg
     6301 atttctccat taaatttcgt tccaatcgtt tctaatgttc gaattgaagt tgttccgaca
     6361 gccacgattt tcccgccatt tttacgtact tggttcaatc gctcagccgc ttcttccgtc
     6421 aaacggtaga attcactatg catatggtgt tcttcaattt tatctacgct gacaggtcgg
     6481 aatgtaccta accctacatg tagtgtcaga tagactaatt caacaccctt tgcttcgatc
     6541 tcatcaagta attctttcgt aaaatgcaat ccagctgttg gcgctgcagc agaaccattt
     6601 tctttcgcat agacagtttg atagcgatct gggtcttcca gacgttcttt gatatatgga
     6661 ggcaatggca tttctcccaa tgattcaaga atctctagaa aaattccttc atactcaaag
     6721 tcaatgatcc gtccgccgtg ttccaattct tctttcacta ctgcttgcaa tctgccatca
     6781 ccaaaagaga ttcttgtccc aactttggca cgtttcgcag gtttgatcaa tgtctcccac
     6841 gtatctcctt gtgtattcgt tagaagaagg acttctaaat gcgcaccagt atctggtttt
     6901 tctccatata aacgagcagg cagaactcgt gtattattca taactagcgc atcaccaggc
     6961 tgtaactctt caatgatttc atggaaatgt ttatcttcca tctctccagt tttccgatcc
     7021 aaaacaagta aacgagaatg atcgcggtct tttaatggtg tttgtgcgat taattcttcc
     7081 ggcaagtcaa aatcaaaatc ttctgttgtt aacatatcgt cactcacttt ctatcatgtg
     7141 cttcatcatt ctatcatgtt tttcgttctg ttggcaaaat cagcactttt tcacttctat
     7201 tttttgtttt cctaaataaa aaaactaccc tatttgtatt ttttcattgt tttaggcata
     7261 ctagtaatag atagttcttc attgaaaata gaaaaagagg tggcaccatg attgaatttc
     7321 aaaatgtgtc aaaaatttat aaaggtggaa aagtcgctgt agaggacgtc aatctttcat
     7381 ttgaaaaggg tgagttcatt tgttttatcg gtacgagcgg aagcggaaaa acgacgacga
     7441 tgcggatgat caaccgaatg actgatccaa cacaagggaa aattttgatt gatggaaaat
     7501 ccatccaaga aattgatcca gtggaattgc gacgtcagat cggctatgtt atccaaagta
     7561 ttggattgat gcctcacatg acgatccgtg aaaatatcac tcttgtccaa aaattgttga
     7621 aagtcagtca agaagaacgg aataaaacag ctgaaaaaat gattgatctt gtggaattgc
     7681 cccgtgagat gttagatcgc tacccacatg aattatctgg tggtcagcag cagcgtattg
     7741 gagtcgttcg tgcccttgca gcgaatcaag atatcatctt gatggacgag ccattcggtg
     7801 cgcttgatcc aatcactcgt gattccttgc aagaccttgt caaagattta caagagcgtt
     7861 tgggaaaaac gatcgtattt gtaacccatg acatggatga agcgattaaa ctagcaagcc
     7921 gtatcgccat catgagtgaa ggccgtgtga tcaatttgat acaccagaaa atattttacg
     7981 tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac tacaagccaa
     8041 gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga ttacaccgga
     8101 aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata cgttgttagt
     8161 taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga ataaaaaacg
     8221 aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg taaaagaatc
     8281 cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat acgtgccggt
     8341 cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag tggatatcgt
     8401 ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt cagctactcc
     8461 tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg gttcagaaat
     8521 tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca ttggtatcat
     8581 cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca cgattgtgat
     8641 tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat tgatgatccc
     8701 gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt cattattgcc
     8761 gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc gggatgtggc
     8821 aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc ccattgctat
     8881 gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat gggctacact
     8941 tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac tgaataatta
     9001 ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac tattggctga
     9061 ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag aggagaatga
     9121 agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat acttgcgagt
     9181 tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat cacaggtgga
     9241 atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga acattataca
     9301 gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca tcaagcgatg
     9361 atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt aacgactaca
     9421 ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa agatgaattt
     9481 gagaaaagat acggtcaaac gtggttcccg tcctatagtt ttgaaaatac gtatgtcttt
     9541 cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga tctgaaaaat
     9601 gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa aggagacggt
     9661 tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc tatgcagatt
     9721 ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg ctactctacg
     9781 gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca attcttccca
     9841 ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc tcatttaaaa
     9901 gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga attgaactac
     9961 gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt acaagaaaac
    10021 cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa caatttttct
    10081 attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac ttcctgattt
    10141 ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc atgatttcta
    10201 ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag acgatccctt
    10261 ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat gtcgtgattg
    10321 ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc gggatgatcc
    10381 aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct agacaacgac
    10441 tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata agaaacgcgt
    10501 tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt ggtcttggag
    10561 atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta gcaggtgcat
    10621 tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg gaaagacggc
    10681 tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag cttctgcgac
    10741 catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata atccactctg
    10801 attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca ttccgtatac
    10861 tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt tttttcttat
    10921 ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc ataccacatg
    10981 atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac atcctttggc
    11041 gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt cagctgcatc
    11101 atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa tcgggaactt
    11161 gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc catgggaggt
    11221 ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca actagacttg
    11281 gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc gatatattgc
    11341 caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt gccgttgata
    11401 ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt gacattcatg
    11461 ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt attgtatttc
    11521 gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca ataagacgac
    11581 tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg ataaacgaca
    11641 tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac tgccaacact
    11701 aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc ttgttttaca
    11761 taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg tggagatata
    11821 ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga acaaaaaaag
    11881 agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc ttctcacttc
    11941 actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca tcaactttgt
    12001 aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt gtaggtctca
    12061 gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga atcacttctt
    12121 caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc gcagagtttt
    12181 tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat cgatattcgt
    12241 aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg tttgctcaag
    12301 aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag cattgtttcc
    12361 actacgatca ctaataatct cgatttcagt atgattttca tttacctctt tcaataactg
    12421 ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg tctctcctcc
    12481 tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac aataaagcac
    12541 ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt tttagggagg
    12601 atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa cgtaaagtaa
    12661 ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag tctatgcaac
    12721 aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct tgaataacct
    12781 gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc gtcaagtctt
    12841 ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca ctatcgattt
    12901 gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac tgagttatgg
    12961 taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca catggcactt
    13021 aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat ttggcaaagg
    13081 attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa taggaagaat
    13141 aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc tttagctgag
    13201 aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt ttttgatttt
    13261 aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata ttttcacgcc
    13321 aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc tgccagcgtc
    13381 tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc gctgcatttt
    13441 tacttacgat atctacataa cggataattg gattttcaat cgagcgataa gaagagtgga
    13501 cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt tctttttctt
    13561 tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg atcgaacgtg
    13621 cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca agaacggtat
    13681 tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct gctacttttt
    13741 tataatgatc atggatctta taaaaaatga acataacgat tggcataatg atgaagaaag
    13801 gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat gagatgaacg
    13861 cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt ttccatttga
    13921 cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag tataacggaa
    13981 tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct agcgctagtg
    14041 tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg tgaggcatga
    14101 atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg gtgttagcag
    14161 caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata ccatgtccaa
    14221 agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg atcccatacc
    14281 aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc atcatcgtca
    14341 atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca ttactaatcg
    14401 cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc aaagcgatag
    14461 aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca gcacctgtca
    14521 cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat gtgtacaccg
    14581 gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc atcagtagca
    14641 aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc gctgatgtga
    14701 tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg atcaataaag
    14761 agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg acatatgctc
    14821 ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat gaaatcaata
    14881 aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca gacaaagcga
    14941 ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca tcagacgaca
    15001 ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca gattttaaag
    15061 gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta cacacaattt
    15121 tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta cgctcactga
    15181 ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa tatcaagcgc
    15241 tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt tttctttgaa
    15301 ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt tttgatatct
    15361 taacttttgg aagagagatg taaggctgct ttctacccat cattcttact actcgaagca
    15421 tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc tgcggtaata
    15481 agcccaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt attacttgat
    15541 gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga ccctcggtat
    15601 tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct ctaatactca
    15661 ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc tcatgcgccg
    15721 cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt cctacgattc
    15781 caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt ggccaataaa
    15841 ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag aacggattga
    15901 tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg ccagagatcg
    15961 acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat gcagccaaga
    16021 catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg ctgtagaccg
    16081 gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc aagctttgcg
    16141 caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt ttcgtaaaat
    16201 tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa agaagaacag
    16261 cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg ttatttgcga
    16321 ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca ctttcttttg
    16381 ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt tcgccttttc
    16441 ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca ttgttgatca
    16501 agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt actgttgtca
    16561 atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa tcagcggcca
    16621 atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc ccttcattga
    16681 tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca tggatttctt
    16741 gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca ttcgttacag
    16801 ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg ctcagtgcgc
    16861 cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca gtggaactga
    16921 tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg gcatcaattt
    16981 gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg ttcaaagcag
    17041 ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga tcaataattg
    17101 gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg accagtttat
    17161 cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca ttgctgatgt
    17221 ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca gtatctatta
    17281 acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct acactgtttg
    17341 tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg tttgctagat
    17401 cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact tgttggatga
    17461 tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg atcgaagcaa
    17521 aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg attttttctt
    17581 gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca ttcaatgcaa
    17641 cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca tttgctttat
    17701 tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt tttgtgtagc
    17761 cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact ttattgatac
    17821 ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat aaagtcgaac
    17881 tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct cctttatcgg
    17941 tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt tttggtttct
    18001 tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc aagtaaatac
    18061 ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa tcaacgaatt
    18121 gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca atggccactt
    18181 ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc agctctccag
    18241 tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc gaagggatga
    18301 tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt ttccagtcaa
    18361 gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt cttctttttt
    18421 cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt tcaaaaaagt
    18481 cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata ttgagaaata
    18541 attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat tttatgccaa
    18601 agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc tttgccccta
    18661 caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt cgaatgacag
    18721 gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga tgacatgctt
    18781 aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt ggtaggcttt
    18841 gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa acgattcata
    18901 aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg caacatacct
    18961 gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt tcgtcgcatc
    19021 gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat cataagcagc
    19081 aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg cactggttaa
    19141 gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg cacctgatgc
    19201 tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga ttagattata
    19261 agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt tatctggaat
    19321 ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc cgactgcatg
    19381 gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct ctgtcacaat
    19441 accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct ctttcatttc
    19501 tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt ctacacgaac
    19561 atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat cttgtgcttc
    19621 tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc tttaagttta
    19681 cgtttatgcc tttattttga caaaaaaaat gtcttttcgt tttggcgcat gattcaaaac
    19741 aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc gtaaagatta
    19801 cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa atcaaagatt
    19861 gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg ccaggaatta
    19921 aaagcgcaaa aagattacga aggcttgcgt aaacttcctt tagatgctcg tccagaacga
    19981 ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa attcgggatg
    20041 tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt aaaaaaagcc
    20101 agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac cactccttgg
    20161 atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag aagcagacgt
    20221 atcttttttt acgcacagct ggtccttttt tatatgtaaa ggctgcatgg ttacttcgct
    20281 atatttattt agtaacagga gtcagtgctt cgatacccac actaattttc aatgctcggt
    20341 caacttctgc cataatgggt cgatccaaat ggcagacctt ttctttcaat cttgatttat
    20401 cgatcgttct gatctgctcc aacaggataa cggagtctct ttctattcct gttccagtag
    20461 ctttgatccc aatgtgagtc ggaagcttag gtttagccat ttttgccgtt atggccgcaa
    20521 caatgatcgt cgggctaaaa tggttaccta gattattttg aataaccaaa actgggcgtg
    20581 ttccgccttg ctccgatccc acgactggag aaagatcagc aaaataaata tctcctcttt
    20641 ttaccatagg atcctcctta ttcttgatat tctcttggta tccgctgtcc taatccgcaa
    20701 gccacttcat agtggatcgt acctaattga tctgctatat cctgcatagt gatttctttg
    20761 tctgcatttt tgccgataag cgtcactttt gttccgacag gaaattcttg aggcaatcgg
    20821 atcatcagtt gatccataca tacacgccca atcgtttcac agtagttccc ttctaccaat
    20881 agagaaaaac cctgcatctt tcgtggccag ccatctgcat aaccgatcgg tatcgttccg
    20941 atccactctg cttcaggagt gatatatgtt tctccatagc cgattccttc acctgctggg
    21001 agttttttga cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa tggataaact
    21061 tccggtagtg cgtgcccaga gggattaagt ccgtacattg ctactccata acgaatcata
    21121 ttgccgattg tttcatcatg ccataaagcc gttgcactat tgctgacatg tacatatcga
    21181 ggaagttcgg gaagtttttt cagcacttct ataaagcgtt ctttttgcag attccaatat
    21241 gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata aaccttccca ttctaataca
    21301 cgactttctt tgatgaatgc agctgcctct tttacttctt caggtgtaca aaatccgata
    21361 cgacccatcc cagtgtctgc ttttagatga atcgaaagag gcgtctctag cttacttttc
    21421 tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac tgataaatca
    21481 tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag gatgggctga
    21541 gtaaatccag cttctctcaa ctcgactcct tcatctaaat ttgaaacaca aaagcccgaa
    21601 gctccacctg caactgctgc ctcagctgtt tcaatagctc catgaccgta tccgttcgct
    21661 tttactactg caaacaattc ttttccttgc ggaagtcttg caacttcatt tgctacattt
    21721 tcagttatag cttttttatg tatcactgct ttcgttgggc ggtgccaagc gacaaccatc
    21781 gtttttcctt ctttctagtc ttccaaaata atttgtgcaa aagctgtttc atttgtatga
    21841 gtaatactga cccatacatt gccttcatga ggtgaacgcg tcacaacagg ctgaccattt
    21901 tcatttttta aaatctcgac atcttgaaag gtaacttttc cgatcccagt tccccaagct
    21961 ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa attctacttg gcgatgaaaa
    22021 ggcaaggact ggaatagttt catttcatca gaggtcaaga ttcgtgcaat gaattttggt
    22081 ttttcttcga tcagccgagt gatcctagga agttcgactg catcgatccc aattcctttt
    22141 atcatatgct tttctctttt caataaattt agaacactct cattctacca aattataacc
    22201 attttgccac aaataagtcc acaaataaca aaaatgaaag tttctcttaa aaaagagaaa
    22261 ctttcatttt atgtttaatc gttatttgaa cggattacaa atccgcgttt tttatcattg
    22321 tgacgtttac cgccatcttt ttggctgtta ttatttttgc tataaccgcc ttttgatttg
    22381 tttccacgat aattaccgcc gtcacgacga ttacggttgt ttccgccacc acgtttgcca
    22441 tttttattgt atcctttttt ctgcattggc aatgggcgtt ctggtgtgat cttcactggc
    22501 actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa atcttgtgca
    22561 gaatattctt ctaataattt atctgctgtt tgcaggtatt tatctagacc attttctgca
    22621 agttttgttt cgatttgttc aactgcagca cctaattgac ctttgaatgc ttctttttcg
    22681 gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac atgcaagtag
    22741 tccatttcat ttggtgtaac gaaagtcaca gacataccgc cttttccggc acggcctgta
    22801 cgtccgatac ggtgaacgta gctttctgga tcttgaggga tatcatagtt gtacacatga
    22861 gtgacacctg aaatatctaa tccacgtgca gcaacgtctg tcgctacaag gatatctaaa
    22921 tgaccgctct taaatgaacg caatacgctc attcgttttt gttgagacaa atcgccatgg
    22981 attccttctg cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc tacacggcgt
    23041 tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa catcaaacaa gcgagtcatg
    23101 atatcgaatt tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa gtcagctgtc
    23161 atttctttcg ctttgatctt cacatgatgt ggatttttca tgaatttaac gccaatattc
    23221 ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc aggaacttgt
    23281 gaaatgattt tttcgatatc ttccaagaag cccatattca acatttcatc tgcttcatcc
    23341 aaaactaatg tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg gtcaagcata
    23401 cgtcctggtg tacccacaac gatatgtgga cgatctttca agccacggat ctgacggcca
    23461 atatctgctc cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc taaacgatat
    23521 aattcttctt gtgtttggat agctaattca cgtgttggtg cgataactaa accttgtaat
    23581 tcgtgacgat ctggatcgat tttttctaac attggtaatc caaatgcagc tgttttccct
    23641 gtacctgtct gtgcttgtcc aataacgtct tttccagcta atgctagtgg aatcgtttct
    23701 gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa caattctggt
    23761 gataattcta gttctttaaa tttcaaatga atcctcctat atggttgtta actgaggatg
    23821 tagctttttt gctaccccgt cgatttgttc ggtcaaagtg tattgtttct ttttgacagt
    23881 ggccacgcac gtttttatcc attcttagcc cctgtggcac caccgaaaaa tcactttgcg
    23941 aagtttaggc attagaaaag ccaaacgggt cgcgttcagc tttatcatta ttatgacatc
    24001 tggcttcaac aagttgtttc tatggcaaaa ccagtccaat ttctcttcat ctttcggcaa
    24061 taggttattc taacatagtt tttcgttttc tgcaagttta cagattttta tttcatttgc
    24121 tgaagttgtt ctaccacttc aaataagccc atgctgttgc tgccttttag cataatacta
    24181 tctgttgctt caagattttg tttgatagca tcaatcaatt tattcttttc tttttcaaaa
    24241 tagaacacag gtagttgggg gtatttttgg ttcaattctt cttttagtgc atgcatttgt
    24301 tccccataaa ggaaaatcat ggcgaatttc tcaggatcga tatgttcact catttgcgca
    24361 tgcatattta aagactcttt ccctaattcc agcatatctg ctaacacagc gattctgcgt
    24421 ccttttgttg gcagattttt aaaagtatct aataccagtc ccatggctgt tggatttgca
    24481 ttgtagacat cgctcaaaat agctgcacca tttcctgccg ttagccattc ggttctgttt
    24541 tgagtgatag aaacatgggc gagcccttgg cgtatctcag gtatggacag tccaaagaag
    24601 cgcccgatcg tatacgcaat caaagcattc gttacattat aacttccagg cagcgggatc
    24661 gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt ttccttttgc tgtcacttcc
    24721 gcatgaacat cgccatttcc aataccgaat gttgtgactg tttgtactaa tgttgccgtt
    24781 aaaggtgtca gcaacggttc atctgcgggc acaacgagta atccgtctga tacaagtccg
    24841 tcagtgattt ccattttggc tttagcgatt ccttctcgcg acccaagatt ttctatgtgt
    24901 gcttcaccga tcattgtgat tgctgcagct tttggttgag ccataagtga aagttccgtg
    24961 atttcattgg cgtgatccat gcccatttcc aaaacgatct tttctgtatc ttggggcatg
    25021 tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat ttccttgcgt cttatatgtt
    25081 ttgtattttt cagacaagac agacgcggtt agatctttcg tggtggtttt accattgctt
    25141 cctgtaattg caacgacttc tgcgcctact ttttccaagt aataggttgc caattgctgc
    25201 atggctttca aaggatctgc tacaggaata actggaaaat tttttggagc ttgttcaaca
    25261 ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg cttgctggat aaattcatgt
    25321 ccatctcgcg taccagcaag aggaacgaat aaacttcctt cggttatctt ccggctgtca
    25381 aattcgacat tggtaatctt tgtcagtgca gagcattcgc tgcccacggc ttgtgcaatt
    25441 tcttgtattg ttagattcat tgagatctct cctaaaaaag gttgtgaaaa aagcgtttgg
    25501 cctaaacggc gggttcgcat gactctcact tccaagtaaa tttctctatt cgtatttaga
    25561 agctagaatg aacgcggtta cgcaatcact ccagcctctt ctttatttct ctttattcat
    25621 ttttttcaaa aaaagattga cgctgatggt atcgattcat tcctaactgg atcagttctt
    25681 caatcaaatc accgtatttc aagcccatat tttcccataa gagtgggtac atactgaact
    25741 ccgtaaatcc tggcatagag tttaattcat tcaggaataa ttcattttta tttgtcaaaa
    25801 agaaatcgca ccggctcaat ccgcttccac ctaacatcgt gtaagctaac ttcgcgtact
    25861 cttgcgcttt ttgataaact tcttctggca cttcggctgg aatctgcatt tcgattttat
    25921 tattgatata ttttgcttca taatcataga atgctacgtc ttttacgact tcaccaggca
    25981 aagtcgtccg aacgtcttca tttcctaata cagcaacttc gatttcgcgc gcttcaattc
    26041 cttgttcaac gattgctcga gaatcatact gataggctgt tgctaaagca ttttgcagct
    26101 cttctcggtt ttctgctttt gtaatgccga cactagaacc catattcgcc ggtttgacaa
    26161 acatcggata aagcaaagaa ccttcacatt gatcaaatac ttttttagga ttttctttcc
    26221 attgattctt aagtactggt acataaggaa cttgcggcac accagcagct tgtaaaatat
    26281 acttggtcat gattttatcc atggcacatg cactggtcaa tacgcctgcg ccgacataag
    26341 gcatattcaa tgtctctaag aagccttgga tcgttccatc ttccccgttt ggcccatgta
    26401 aaactggaaa aacgatggct ccttcttctt tgatttcgcc cggattgatc acttttcctg
    26461 taaagccttc ctctgtctgt ccacttgggt cccatgaaag atgcaagaca tctttgctgg
    26521 caggtttttc tgttagtaat ggacctttga cccattgtcc ttcttttgta ataaatacga
    26581 gttgaacttg gtaataatta taataaatgg catttaaaac tgaaaatgcg gaaagaatgg
    26641 acacttcatg ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa agattgtcct
    26701 cctgtattct aaatcgttca gtgatttact ctcatatttt agcacaaaac aaaaataaac
    26761 gaataactta tttataaaaa aacgagccag cttctaaaag aacgagcgtc atttattttt
    26821 tgtcagctag attatttgtc ccgtttttta taaaaagtta tgataaagaa aaggagggat
    26881 gatgatggcg caaacaaatc cacgcagccg cttggcagaa atcattcgag tgttcatcaa
    26941 ttacaatgta gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa aaaaagcctt
    27001 tgaagaacta ggccccacct tcatcaagat aggacagatg ctttctgttc gagaagattt
    27061 gctttcttct gccttcactc aaacattcaa aacattacaa gatagtgtac ctagcgacac
    27121 attttcaacg gtcaaaaaga cgatcgaaac agaactttct ctttcattat cagatatatt
    27181 cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc atcgtgccaa
    27241 gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg ccgaagagat
    27301 ccgacttgat cttcaactat ttgaacgagc gatccctttg atcaaataca ttcctgaaac
    27361 tagcgtagtt gatctaaaag gggtactcca agaagtaaaa cgttccttga tcaatgaaat
    27421 ggacttctta aaagaaagtc aaaatggcga acaattctat caaaagaaca atggctggaa
    27481 agagatccgc agccccaaga tttatgacgc tttttgttcg aaaaaagtga tcgtgatgga
    27541 agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa ctgaaacttt
    27601 tatcactgga attcaaaaca agcagctgaa acaagaagtt gcaaagcttt tagttgagaa
    27661 ctttatgaaa caggtgtttg atgatggttt tttccatgct gatcctcatc cagggaatct
    27721 gctattccat gtactaacaa aagaagaaca gacacaagct agccgaaaaa ctgaaacagt
    27781 ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc acttctgcag aagaccctgt
    27841 tgcgccatat accatcaact acatcgattt tggtatgatg gggcatcttt ctgcaggact
    27901 taggcaaaaa ctcacacaag ccgttttagc gctgtatacg aaagatgctt atcgaatcga
    27961 aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc ggttccacca
    28021 agaactgaca agttttcttg aacagtatta tgattctcct attgatgaaa tcaacttgca
    28081 agaagtattc acacatgtcg tgacgatttg tcatcaaaat aatctgcagt tcgatcgtga
    28141 tatcacgctt ttgctgaaag catttgggac attagaaggg gtgatccgcg tcctcgatcc
    28201 tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa cattattttc tcactcatct
    28261 ggatgtagaa gatacattga aacaaagcgg attagactta ttggaaggga tgaaggctgc
    28321 gcctaagatc cctcaacagc tccaccatct attagaaatg tggacttcgg gacaaggaaa
    28381 agtcaatttg gaattgaaaa agcaagacaa attattgagc aggatcgaaa gtatgatcaa
    28441 tcggcttgtt ttcggtatga ttttagctgc attgatcgta gggtcctccc tgcttgtcca
    28501 agcagcacct gtagaaaacg cagaggtcgt ttccttatta gggatattta cgtatgctat
    28561 tgccgcattc gtcattattt ttttggcaat cgatgcattg atccaaatgt ataagaagag
    28621 gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc aagaaataag aaggggtttt
    28681 acataaattg tttctcaaat ttttgtgaaa attcggctta tttttcgaag aggctacttc
    28741 tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc acccagtaat aagaacaatc
    28801 aaaaagggaa catggaacac atttatcctg tttccctgtt ccctttttga tgtattcgag
    28861 cataaataaa ataccttcgt tacctactat cttctgtact tggtaaacct tagtcaattg
    28921 ccttttttaa cgcttgacga aaagacatat gatctttctc cgtaaagggt tttggaccct
    28981 ttgtccttat gccagctttt cttaactttc cactatgata acgttgttct agaagcgtgc
    29041 cgatattcgc ttcgtcaaat tcttttccag agtgatggaa cactcgcaca cccttcggca
    29101 atacaagtga agctaaacca taatcttgcg tgatcaaaaa gtcattcttc ttaaccatac
    29161 cgacgatttt ataatccgca ctatcagctc cgcggtctac gtagacgatt tggacatgat
    29221 tagcgtattc tttggtcgta taatggtcca cactggtcac gataaccacg tcaagaccat
    29281 aggattctcc taattttatg acatcttctt ttactggaga accatctcca tcaatgaata
    29341 gcctcattct ttttcgcctt cttccataaa tgataaacag ttactaatcc gctcagtaaa
    29401 agaccaacat tgatccattt tgccaagata agcagttgta cactttctat cggcactgat
    29461 tcatggttca gcgtgatcaa taatagatgg attggctctc ccatttcttt tgctaaaaca
    29521 aacaaaagcg tcatcaataa aaactggtgg atcacactcc ctttattttg agtagtatac
    29581 ttgacaacga tccggcgatc cattggaata gctgtatcag tttgataata ttccttattt
    29641 agccagctgc ctgttactac gtgccagaaa ctaagcagaa agacacctat cgcaaaacca
    29701 tttggaatca agaggaccaa taaagaagaa ctcagtccaa gcaattgcat agccaatgga
    29761 gaccatgtta aacgtttcaa aatccatggt ccgactattt ttgctgcaat caagcctgct
    29821 gcataaggca aatacataaa aatcgaaagt ttttctaatc cgtacagtat gccaacataa
    29881 aaactgctaa ataaaaaaag gaaattccct agcattccat ttagaaaagt aaacagattt
    29941 agccagcttg gcaacttcca ttccttcttc tggtgtccta aaacaaacac gaacaagatg
    30001 aacagcagca agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa taacaatcgg
    30061 gcatttctca aaagaaacaa taagccaaag aatatcaaaa ataaaacaag ctctcgcgta
    30121 gctaccagat ttcttttgat gtctttgaaa tccagttcgt aacgcggata atgactaacc
    30181 gtatggtagg ccatgacgta aaaaagtgta taaatagtaa agcttgctgc aatctgagtc
    30241 ggtgcaggca actcaatcgc ttgatacagg agaaatagcc agatagctgc aaataagtac
    30301 tttttcccat ccatattgat aaaaccttgc gtcttttcgt gaaaattgac cgtggtatta
    30361 gcaggtacaa gccatgctgc actcagccct agcaaaacac ttgaaaaata aaataaagga
    30421 aaataaaata cgcctaatat tccaagcaat gaaccagcac caccaatcag cagagcgatc
    30481 atcagcaacg tgtaactatc tagaccaaat cgtagactgt tgactaaaaa tagtccagtc
    30541 atccgaaagg cataaaacag cgtaaatggc aaaatcgtca gccaattatg cgagtctgtt
    30601 agtcccaaaa ataaaagata aggaataaaa ataaccgtgt tacttaagat aaagggcgtt
    30661 gtttgtgtaa aattacgccc aaatcgtttg agtttttcga acatgacaat ggctccttaa
    30721 acagtcttat aactaatctg ctcttcgatt ttagcattaa aggggaaata aaccaatttt
    30781 acagaatata ttcttgtaaa aaaaaataca gaaagaagca taagcttctt tctgcatttt
    30841 tacggtactt gattactcaa gcagacaagc ccttctccac aaaagtcttg cccgacaccc
    30901 ctgcatccgg caggtgataa agatagaaat caattatcct gtgcctccaa aatcaattag
    30961 gagctctgta aaccagagtc cttctcgacc cgtcgcataa atttacttta acacagttcc
    31021 tatgtttatg cgagagcttt tttcatttta taatcgacca tttgaaatcc ttgaccgata
    31081 tctgaaataa gttcttctgt aatctcaaat ccttcatgct tataaacctc gattgcttga
    31141 tgattcccct gattcactcg caaaaagatc tctctttcct ttgtttgcgc agcaagttct
    31201 tcataccagc gaaaaactga agaagtcaat ccttgaccac gtactttatt tagcaaatat
    31261 aatttactta aataaatttt tccatttatc tcttcataag cagtgtaacc gacagtcttt
    31321 tcttcgcttt ttaataaaaa gtaggacaca ccttctgtca gttctttttg aatctgtttt
    31381 tttgactgat aagttttcag catgtaatcc acttgttttt gaccaatgat cggcacaaaa
    31441 acttccggcc atacttcttg tatgactgcc atcagctcat ccagttcatt ttctgttact
    31501 tttatttttt ccatacaccc acgtcctttt ttacattata aatgaccttg cgcttgatgg
    31561 aataaaaaga gcatgatcat gctatacaca cccaccgacc acggtttcaa gaaaatcaca
    31621 atctgcataa attccctaaa agataatttc gtcaaaccag ctactagaca gatcaagtcg
    31681 tctggcgcaa agggaagcag caaggtaaca actagtactt ttttaatatc tttcgttttt
    31741 cttgtcagca attcatcaaa tttctgatac ttttttggcg ataaaataag ctggacaaag
    31801 gctacgccat agtaacggac aagcaaaaat ccgattgctt ccccaatcac taggccaata
    31861 caactatata aaagcccaaa tccattgcca aacatcagca tgcccgctac tgaggagatc
    31921 cctcccggca aaataggaat gatcacttgg atcacttgca acagaacaaa tatcatcact
    31981 gcatacttgc caaattgctg gataaacgct tgcaaagacg tgctcgatcg aaaaatccca
    32041 tgatgataag cataaagaat caacacaaag aaaaaaaaca gaccaataat gggcatgatc
    32101 tgaagacatt tttgtaaaac tgatcctttt ttagacatct atattcccca catctttttt
    32161 catttattaa aactgcctc
//
LOCUS       DAZKLA010000022        31270 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000022 DAZKLA010000000
VERSION     DAZKLA010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.022"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(372..1682)
                     /locus_tag="ACG85N_000828"
     CDS             complement(372..1682)
                     /locus_tag="ACG85N_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFQ0223622.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1694..3052)
                     /locus_tag="ACG85N_000829"
     CDS             complement(1694..3052)
                     /locus_tag="ACG85N_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0223623.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
     gene            complement(3254..4822)
                     /locus_tag="ACG85N_000830"
     CDS             complement(3254..4822)
                     /locus_tag="ACG85N_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFQ0223624.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4826..5236)
                     /locus_tag="ACG85N_000831"
     CDS             complement(4826..5236)
                     /locus_tag="ACG85N_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0223625.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5233..6156)
                     /locus_tag="ACG85N_000832"
     CDS             complement(5233..6156)
                     /locus_tag="ACG85N_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFQ0223626.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6153..7031)
                     /locus_tag="ACG85N_000833"
     CDS             complement(6153..7031)
                     /locus_tag="ACG85N_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFQ0223627.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7018..8355)
                     /locus_tag="ACG85N_000834"
     CDS             complement(7018..8355)
                     /locus_tag="ACG85N_000834"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFQ0223628.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8358..9461)
                     /locus_tag="ACG85N_000835"
     CDS             complement(8358..9461)
                     /locus_tag="ACG85N_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0223629.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9535..11043)
                     /locus_tag="ACG85N_000836"
     CDS             complement(9535..11043)
                     /locus_tag="ACG85N_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0223630.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11009..11701)
                     /locus_tag="ACG85N_000837"
     CDS             complement(11009..11701)
                     /locus_tag="ACG85N_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFQ0223631.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11723..12697)
                     /locus_tag="ACG85N_000838"
     CDS             complement(11723..12697)
                     /locus_tag="ACG85N_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0223632.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12997..13869
                     /locus_tag="ACG85N_000839"
     CDS             12997..13869
                     /locus_tag="ACG85N_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0223633.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14327..16525)
                     /locus_tag="ACG85N_000840"
     CDS             complement(14327..16525)
                     /locus_tag="ACG85N_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0223634.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16757..17749
                     /locus_tag="ACG85N_000841"
     CDS             16757..17749
                     /locus_tag="ACG85N_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0223635.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17809..19230
                     /locus_tag="ACG85N_000842"
     CDS             17809..19230
                     /locus_tag="ACG85N_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFQ0223636.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19223..20722
                     /locus_tag="ACG85N_000843"
     CDS             19223..20722
                     /locus_tag="ACG85N_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFQ0223637.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20761..22155
                     /gene="uxaC"
                     /locus_tag="ACG85N_000844"
     CDS             20761..22155
                     /gene="uxaC"
                     /locus_tag="ACG85N_000844"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0223638.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22314..22997
                     /locus_tag="ACG85N_000845"
     CDS             22314..22997
                     /locus_tag="ACG85N_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFQ0223639.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22999..23835
                     /locus_tag="ACG85N_000846"
     CDS             22999..23835
                     /locus_tag="ACG85N_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0223640.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23846..25600
                     /locus_tag="ACG85N_000847"
     CDS             23846..25600
                     /locus_tag="ACG85N_000847"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0223641.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25876..26217)
                     /locus_tag="ACG85N_000848"
     CDS             complement(25876..26217)
                     /locus_tag="ACG85N_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0223642.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26285..28456)
                     /locus_tag="ACG85N_000849"
     CDS             complement(26285..28456)
                     /locus_tag="ACG85N_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0223643.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28667..29524
                     /locus_tag="ACG85N_000850"
     CDS             28667..29524
                     /locus_tag="ACG85N_000850"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0223644.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29595..30395)
                     /locus_tag="ACG85N_000851"
     CDS             complement(29595..30395)
                     /locus_tag="ACG85N_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0223645.1"
                     /translation="MTIKKKLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGID
                     KTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNS
                     CIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAA
                     GPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLL
                     QKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
     gene            complement(30392..>31270)
                     /locus_tag="ACG85N_000852"
     CDS             complement(30392..>31270)
                     /locus_tag="ACG85N_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0223646.1"
                     /translation="AHCLNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKSNCGRRPLV
                     LPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRK
                     PNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKV
                     IITLKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFA
                     DPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRKSLDYRTPL
                     EVFLSYVSIDDLSNLI"
BASE COUNT         9347 a         6427 c         5530 g         9966 t
ORIGIN      
        1 gagtgtttcc cgattcgttt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt ttaacgactt agttatctta tcacgtctaa
      241 aaacatgtgt caactctttt ttggaatttt cataaaatgt ttcaatgaag cgcgacaacg
      301 ttgtttaata tatcattaaa aaaaataaag gtcaagctaa aatattattt aagcttgacc
      361 ttattataat ttcaacgatt gtcaaaagta ttgtttgcag ctgtgttgcc agtttctata
      421 gataaatcac agcttttgaa acataagttt tgtactgctt cgattcgccc gaattctttt
      481 gcagttattt ggcaatgaga aaagcagaca tcttctatag gcttttcagg aaatgctact
      541 aaatcaaaag ctcgggaagg aagtgagtaa cctggagaaa ggcatgcctg tacgtttttg
      601 atgtgtatgt ctttgacttt cgtcatttgc tttgattcag gtatttcttc agccactgct
      661 tgccaacttt ctggcatttg agcaaggtca ttgctatttt ttctattgta ttcattatgc
      721 cagtccatta tccaagaaaa aggaaattgt acatcaatca tattcagatt ttctaccctg
      781 atattttcga tcactccccc tctttctttt gaagatttca ttcgaatacc acagtctgac
      841 tgaaaaaaat cgatatcgtg aatatatacg tcactaactc cagcactaac ttcgctacca
      901 atagttacac cgtatcctga acggatttta catctagcaa cctcgattct tgatgatttt
      961 cgattgactc ttgcaccatt gccatctctg ccagacttaa tagcaataca atcatcacca
     1021 caggccaaat cacattcata gacacgcaca tttgttgaag aatcaatatc aatcccatct
     1081 gtacttggtc catcattgtt gcggatgatt actttttcta ctaatacatc gttagaataa
     1141 gtgatttgca agttccaaaa acctgatttc tgaaaaatca aatcacgaac aacaacatgt
     1201 tctgcatgat acagtaaaca agcgcgtgga cgtttaatag cataatccgc aatccaacga
     1261 agtccctttt gatcgtattc agctcttgtc ccgctttttt gatcttttcc ccaatacagc
     1321 tcccaccaat ggtctccttg accgtcgatt attccttttc cttctataag aatatctttc
     1381 gccgagagaa cattcaaaat cgctgctggc catttcattt ctacgccggc tactcgtgta
     1441 tcgatttctg gaaatgcttc gatatctgtt ctccccttga ggactgcacc ttcttcaaag
     1501 ataagatgac tcttatcttt taaaaaaagg gctccgacta aataaatgcc ttttggaata
     1561 aatacaggta agtgttcttt cgcaccttga tcgattgctt tttgcagtat tattgtatca
     1621 tcttgttgag tgtcgtttgt tttaaagttc aaagcattga taatcccttt ttttgtccgc
     1681 aatacgtttc tccctatctt tcattgattt ttttcaattc ttcaccaata aatacagttt
     1741 ttgaacgatt cagtgggtaa cctactagca aaatgattgc ggatacaaat aagcagaatg
     1801 ctggtaacaa gtttgccatc gtataaatcc tttcttgtac taaaactgat tggatggctc
     1861 cacctgttgt agacgattgg taaccaatga gtgccagcat aaatccgcct accgctcctg
     1921 cacatgcttg tcctactttt ctcgcaaaag aatttactcc atatactgtt ccatcttctc
     1981 ttaagcctgt cttatattga tgataatcaa tcacgtcagt aatgaatgcc cagaccatca
     2041 aattgaataa accagatcct aaagtagcga taaataaaag aatcagatat acccatgcat
     2101 ttgttatatg aaggaaatac atcagcaagt acatacatga agaagcaagt aacgcaaata
     2161 cgcttgcttc ctttttacca aataattttg tcagctttgt agcaaatggt gctaaaacta
     2221 ctactgttcc gtaagtaaat aaaagagcaa cagacattgc ttgtttatta tggaagtaat
     2281 cattgaataa ataagtcata ttcgtcccag ctaaaatctg attgatcaca atgaaaatgt
     2341 caacaatgac taaaataatc agtgctcgat tagctaacaa accttttacc aactgagaag
     2401 cagatacttt ttcgtttttg acaactctta ctctttcagt agtcaattta caagttaaca
     2461 tatatgcgac aaatgctatt gcagcacatc cgattgaaat ccaaaagaaa tgattccctg
     2521 aaacgacttg ttgtccactc ttgttatggg tatacattaa aatcggaatg aagaatcctg
     2581 tagttgatcc gccaatcgca gaaccaatgc ttcggaacgt ggacaacgag gtgcggtcat
     2641 taggatttga gctaattgct gctgccattg ctccatacgg gatattgatt gttgagagaa
     2701 aaatcccata aacaatgtat gaaacaaaaa tataagcaat cttcaccgtc attggaagat
     2761 gttggacgaa tggcaaaaac aaaatgacag tcactaagca aaatggatac ttcatttttc
     2821 tgatccaagg aataaatcgg ccagtttctg ttaactctga gacatcacat aatcttccaa
     2881 ccatcacatc agcaaacgca tccaaaattc tcgaaatcaa gaataacgta cctacgattg
     2941 cccctgaaac gccaagaaca ttcgtataat aaatcatcaa gaaactattg actaaaccta
     3001 aaataaaaca gttacctaga tcaccgaaca tgtagcctat cttatcggaa atcccaaatt
     3061 cctttttttc ttgtttttat attcacaatg ttggcttttt attgcattat cttgacagat
     3121 aaaaaacaaa aaataatcta aaaacattga tgcataagat tgataacttt ttttacataa
     3181 caatatatac aaacaaaaaa attcactaac tagtgaaaat aagttagtga acctcattta
     3241 ttaaagaatt aaattacaga tgatcttttc catcatactt gaaaaactca aaatcagcat
     3301 aacttcctct ttttttattt agatcatgca ctgctatccc gataaaattt ccagtaaacc
     3361 ctcctgagag tatacacatg tttttttgcg gacctacttt cttccattct tcagttgata
     3421 cgtctttcaa atgaaactgt gctgtctctt tctttacagt gactttcaaa aaagtcttct
     3481 tttctgtgag tggaatcgtc caatcttcta tttgtgctac tccgttttct gattctatca
     3541 tacgtaggca ttttcccttg tcctcattcc aagtaacata acaataaata taattggatt
     3601 catttaagta cagtaccagt ccagccatct gattaaaatt attaggcatg tattcaaatg
     3661 ctgtctccgc atcaaaagta aaatccgttt gccgaatagc taacaaatga tgttcgaagg
     3721 tagattgaag agattcaccg gaaatcagac gtaaaaaacc tggtctacat tttgtatcac
     3781 accaatcagc atttggcaaa atgcgtctgg cattccagtt cttctttagc tctccttcaa
     3841 atgtatccat aaactgatga tctttttctt gttgtacctc ttcatttgtc tcaatcatta
     3901 tttcttccat cggagttgtc tggctatctt taagtcttaa ccaatcgtcg gacgtccaat
     3961 acacttcttg aatagcggtt tctcttccca aaatagccgc accgtttaat ggtctggtac
     4021 aaagatatgt catgtaccat cttcctgacg gggtttgtac taagctccca tgtcctgtac
     4081 attgcaatgt agaagcaggt ttgtcactag ctgtcagcat aggaaaacca ggatcaagct
     4141 cgtaaggacc agtaatattc tttgctcggc aaactgtaac tgaatgtcct cttcctgtac
     4201 cgccttctgc cgtcatcaaa taataatacc cgttacgaaa atacaaatgt ggtgcctccg
     4261 ttttcgctaa ttctgtacct gcaaatattt tatagatggg accatcaagc ttcttcttct
     4321 ctgcatcata ttcttgtaat acgataccgc tggatttatt cggtgtgtcc agacgataat
     4381 cccatagttc attgacaagc catttttttc ccgtttcaag atcatggaac aaagaaggat
     4441 caaagccgtt actatttaaa taaattggtt cggaccaagg acctgtgatc tcagatgcag
     4501 tgatcaaata attgtggaca tctttaaacg gacggcctgc acttttgaca tctgtgtaaa
     4561 caagataaaa caatccatca gcataactga tctgtggcgc ccatatactt ccatttgtag
     4621 gatttccttg caaagatact tgagtggtca aaatatccgt tgtatgtgac cagttgacta
     4681 aatttttcga ctcatatact cggattcctg gaagccattc aaaagaggag acaataatgt
     4741 aataccgctc tttcactctt aatatattag gatctggatt gaatcctctt aaaataggat
     4801 ttttcacttt gatagtctgc atgattcagg ctccttttct cattcttact cgatataaac
     4861 tgaatgcccc agctgcgaaa taaaaaaatg caaaaggaaa cgcaataaac tgagtgccaa
     4921 tcaacaatat gaatcctgtt ataaaataca aaccagtggc agttgttaag tattttcctt
     4981 tagcagtcaa aaacaatgct actgctaaaa aagcaagtga aataaacaac acaacggcaa
     5041 acagaccagt catgtcctgt aataatccaa atgtattttc taaagacaaa gttgaatgat
     5101 ctacagcaag cgactgataa ctttttttaa actctgacgc agacatatta cttaaagtaa
     5161 ctgctaaccc gccaaagaaa atcaaagata gactgccacc gaccattcct agcaatagtt
     5221 cccattttct tttcatttct gaaccccctg cttttctaaa tgtatccatt caggtctttt
     5281 gatcgatgtt tcataattgt tattacgttc agagaatttg acagtctgtt catttttctt
     5341 gtcatcccaa ttatcccaac cagttgggtg aacatgccca cctatcttac acgcttcaaa
     5401 taagacattt gcatacggac gccacggtct tcctaaatag acgtttctag tatcaggttc
     5461 tgcagtcaag gtacagcttt caaaacgata ccctgttttt tgatttttcg gcgtagatgc
     5521 tgctgtgatg tatccaggat ttttactggc taagctatgg atatgacact gatgaaaaac
     5581 ggcatctgcc ccaccaaata tgaaatccac cgttccttca atccagcaat attcataggt
     5641 ctgacgacaa tactcaggat tggcagaagc cggtgtacaa aaaggagtac catctttttg
     5701 caaatcaggc aacggacccg tgcataaggt atcctgataa ccagatagtc gacagttttt
     5761 taaagatacg cgatctcctg aattgaataa agcaacagcc tgtcctacct tttctcctgg
     5821 acctgctgta ttttcgatgt gaagattttc taatacgaca ttcgatcctt ctaagaaaaa
     5881 agtagcactt cggaaagtcc ctaactggct accgtcaaga tactcctgtt ttgcaaaacg
     5941 attaccggag ataactactg ttcctgctcc cacaagttta aaattggata atctgctttc
     6001 tatatattca tcatatcttc cttcttttac tatcaagacc tttggatggc tatccggcag
     6061 gcctgccaaa taatcgattc cttcttggat agtttcaaaa tccgtcgggg tatctatagc
     6121 attctttcca attatcagtt tgtctttcat cctcataaat gctcccttat gaattgtttc
     6181 caaatatttc tcatttcttg tgtttccata tgaccgcctg ttaaagaaaa agaagaaaag
     6241 ttttctggac tacccattga ttgatacttt ttgtccagcc cttttctcag cttcagtacg
     6301 ccttcgtcca agcacatcgt atcgtttttt ccaactaagc taagacgagg gcgcggagcg
     6361 attttttctt ggattgctaa cgttgtataa gctgtcaata gatttggcac atagtaataa
     6421 aacccatgat gatctaggcg gcgatgtagc aaaagtattt cgatgtccac ctgtgccgct
     6481 agatcaatac aaactttgac acgagaatcc aatgctgtca cccaccagct taataagcct
     6541 cccatagaaa gaccaatcgt tgcgagccgt tgagtatctg tgtcttccct tgtctccaaa
     6601 taatcgatca agctgatcac atcataaata cgcattcccc ataacgtttt tcctgttaat
     6661 aaaaattgtt tgaacaactc gctttcagaa atacctccgc gttcttcaaa tccccaagca
     6721 tcaatcgacc aaacagcata tcccattcct gtcagtactt ctgcaaaaga tggagaagct
     6781 aagtaaggag caccatggat caattcactt ttcccgactg aaaaatctcc gccgtgagaa
     6841 tgcaggtaaa ggaccggcgg aaacggtcct tctttttctg gataagcata gagggctggt
     6901 actgtttcca attcatttaa gtacaattga tagtattcaa gcataaaacc gttttgtttt
     6961 tctcggtata atcgttttcc actaggggta tgtctgctag atggagcacc taaaaaatca
     7021 ttcaactgca taagcaaagt ttttacgaac aaccagttca tccatatttt ctgattgatt
     7081 tgattggaat ttctcaatac tgatttcttt agtattttct aatagaaatg ctggtccctc
     7141 gtgattttca atcgacatct ggttaaagga aaccttaccc gcaaagccaa tataaaatcc
     7201 ttgtctgctc atttcctcta ttcctgtcat catagcagga acaccgggaa ctgcatcttg
     7261 ggccatcgag acatcgatat tgtcaaatgt gatttcttgt atgaattgtt ccgctaatcc
     7321 ataaataaaa cctgcagaag catggacatt ccttgccgtg atattcgaaa aatggatcct
     7381 tctgaatgct ggtgtccgtt catctatcgg ataagccttt ttttcccata catacggttc
     7441 tttccctcgc ggaccacaga aataataaag attcagtata aatggacaca tcacattatc
     7501 catgactata ttggatactc gaatatcttc aacgatcccg cctcgacctc gtctagattt
     7561 caatcgtatt cctcgatcag tttcttggaa gatacaattt gaaatggtaa tattacgaat
     7621 actaccactc atttcacttc ctaatacgac accgccatgt ccatggacca tcgtgcaatt
     7681 tgtgatcgtg atattttcgc aagcgatcct ttcgtacgta tcttcagtcc ctgccttgat
     7741 tgcaatacag tcatctccca cgtcaatatg gcaattacta atacgcacat ttttgcatga
     7801 ttctggatca attccgtctg tatttggtga atccgctggg ttcaagatcg tcaagttatc
     7861 aaacgttgca ttactgcaaa gaatcgggtt gatcgtccaa cttggtgact ggatcaattt
     7921 aatatctttc acagtaattc gatgacagtt atggaaactc atcaatttag gacgaggata
     7981 agcgagatta tctggttcat ttctaaaagt atgccaccat ttttttccat ttccatctaa
     8041 tgtaccgaaa ccagtgacag agatgttctc tacattttga gcatagatac aggaagcata
     8101 aacttttcga tggactcctt cccaacgtga atgaacaaca ggataatctt ttggatcgtc
     8161 agagaatttc agaaccgctc ctgcgcttaa atgaagttcg acattgcttt ttaaaaataa
     8221 agctcctgtt agaaattcac ctgctggaac aactacggtc ccgcctccgt cacttgctgc
     8281 agcatcaatg gcttgttgta ttgcttcagt attcaattca tcaattgatg cgccaaatgt
     8341 caaaatatca tacatattta taatgcctcc acttcaatca atgcttgcaa gaatgcacca
     8401 acgccttttt gatcattaca aataattggt tcactgatgt agtaagcata tgaaccatct
     8461 cgctgatctg ctccgcctaa ccctgctact tgacagtttt tattcaagtt cagccagcct
     8521 tcttttgttt ccagaacaaa ttcatcaagt aatccttgat gtgatttctt cacttgtgga
     8581 agccattctt ctttgttcag cagtcccaaa cgtattcctt tagccattgc gtaaacaaac
     8641 atactactgc cggaagcttc cagataattt cctttccgtt ttgtttctgt tgttacttga
     8701 taccatgtcc cgctgttttc ttcttgatat ggaagtaatg cagccagtgt cttttctaga
     8761 atttctttca gcaaagaaca atctgtttct tcatgaagta attcataggt atctgctgca
     8821 gccatcaaat accagccaat cgatcggctc caaaaatgat gggatagacc tgtttttgga
     8881 tcagcccatg gttggacctt cttttcatcc catgcatgaa tcagcaaatt tgtttttgga
     8941 tcgaatagat gtttataact gatttcaaac tgtctcaaaa catcttggta atctttttct
     9001 tcactaaaat tctggataaa ttcagcataa aaaggagaac ccatatataa gccatccaac
     9061 caaatttgat aaggataaat ttctttatgc caaaaagctc cttcggatgt gcgtggatgg
     9121 tttgccaact gctgggaaag gagttctgct gcttttttat atttttcttc gcctgtttct
     9181 ttgtacaata aaaagaaaag ctttcctgta tttacatggt cgatattgta ttcttcaacg
     9241 ctgtagccac ggatcgttcc atcttcttgc acgaaatagt cgatattgtc ttttatgaaa
     9301 tcaaaataaa ttttttcttg ggttcgttcc caaagtaatt ggaacccttt aagtacgacg
     9361 ccatagtcat aagaccattt tcctttatac cacttttctt catataagcg aggtgttctt
     9421 tccatgatac tttttgctaa tttctttgac cagttttcca ttatttgctc tcctttaaaa
     9481 attaaaatag gatttgggac aaataccaag aagtatttgt cccaatcgtc tatattactg
     9541 tgccgctgct ttgtatgctt cctcgtactc ttgtgtgatt cgatcgcctc cttgtgcttt
     9601 ccagtcttct acagcttttt tgaatccatc ttcgtcaagc tgtcccatga tgtattgcac
     9661 cgtagcatca atgacgattt tttcaatttc tgatcctact tcattgttcg tcggtgaatc
     9721 caatggcaca gaaggatcca ttacagcaaa ttcttcattt tctttgatca attgattaga
     9781 gaattctttt tcagggtctg tatctttgaa agagaacgtc acttcactag gacgagagct
     9841 tgataacggt tgcacctctt gttgccaaag atccatatcg ataaattcga tagaaccatc
     9901 atcattcaat tcataatgcg ttccttcgat accgccagtc atcaatttat acacatcttc
     9961 ttctaaaaga tcatcaacaa attgaagtaa tttcttcaat tgtgcttcgt cttttacttg
    10021 agatttggga aatgctagca agccgccaat accatttcct tctgaccaaa catgataacc
    10081 gccgccttca ccatctgaga tcttgtttac aggcactaat tccatatcat cttgaatacc
    10141 ttgacccatc gtcttcagat tcttgatatc caccattcca gtgtagattc cagttcttcc
    10201 ttgtgcaaat tgttcttgct gatcagtctt ttgggtaact gcaaagtctt gtgccaaata
    10261 gccattctca aatagatctc ttgaaaaatt caaagctttc atatactctt ctgtatcaaa
    10321 ttctggagtg aatttgccat tatcgtctac agcccagcca tttggtgctc caaaataaga
    10381 agtgaaaagt ttaaagctgg tataacgaat atcactaggc gcacgatcgc caaaaccaac
    10441 tgtgtcgtcc acaccatttc catcaggatc gttttctgta aattgtttag ctacttcata
    10501 aagctcatcc atggtagttg gaacatctag tcctagatta tccaaccagt cttttcgaat
    10561 gactaagcca gcacgagcgt agtctttttg tatcggtacg ccgtacaagg caccgtcgat
    10621 actcgcagct tcgattctat cttcagaaat ttgacttaaa tttttatagt cttttaaata
    10681 ttctgataca tcccagaata cgcctgattt taacgcattg cggacagaag agtttttcaa
    10741 catcgcaatt tgaagtgtaa cgatatctcc tagttcatca gaagccaatg cagttgttaa
    10801 tctttcttct tttgaagcat caggaatcca gttgaatgtc agttccgtat cggttttttc
    10861 ttcgatcaag ttcacaatcg tgtctgtagg cggtgaagca tgatgcaaaa tatttaacca
    10921 agtgatttta gcttttccat cttctgtctc tgaagaactg ttacttgaac tgctgccgca
    10981 tgcggcaagt gctagaattc ctgcaaaact aagacctaat tttacgagcg ttgctttttt
    11041 cataaatttt ctccttcact gttttaataa tatttttgtt ttcttctgtt ttttctgtat
    11101 atgagttctt ccattccaaa tggcaaaata tagaatttgc tgtccacgtg actgcggaaa
    11161 agaagagact gccaccgata aaggttaaaa cacctggcat tgtttggatg atcatcagat
    11221 aaatccctac catacttaat atcaacacta atgagtaatg aagatgtgag aatccgaata
    11281 aaaatgccat tttcattttg aaaaaatatc ctttccaatc ataatgagcc atgattggga
    11341 aaatatagag tgctgagagc acatatatga acgcagcaat atacaaagcg atcctaagat
    11401 accaattgtg tatgaaagaa agatccacaa gtaaaacaat cccgatggcg ccatatatcc
    11461 agctcatcag caaggtttca cgatagtttt ctttaaaata tgtgaagtac gttttcgtca
    11521 aaggaaacgt ttcttttgag cgcagccatt ttctaatcac tgcaaccatc gcatacgatg
    11581 caggaccgaa agtaaaaact cccaaacctg cgatgactaa accgccccac agaaaattca
    11641 aataaaccaa ctgtaaaata cgatttagcc ttccgtttat tttatctaat gactgcagca
    11701 tatctttccc tctctttcta tcttagtaaa cgccgtcttc tcctagcttt ttggcaatct
    11761 tattagctaa aattaccata aacaatccta cgaccccttt aaacaatccc attgctgtac
    11821 tgaaacttaa ctgtccattc ttcaatccag ctgtataaat ataggtgtcg aaaatttctg
    11881 caacgcttcg attcaatgaa ttcaataaca agtacatatg ctcgaaccct aagtctaatg
    11941 tctgaccgat ttttagaatc agcaaggtaa tgattactgg acgaattgcc ggcaatgtaa
    12001 catgccatgt tttgcgtaag cgtcctgctc catccatttc tgcagcttca tacaactgtg
    12061 tatcgactgc agttatcgct gaaaggtaga tgatcgtaga ccagcccaac tctttccaga
    12121 tcacttgtcc gatatatact gtgcgtgtcc attcagggga tgttagaaaa ctgatttttt
    12181 caaatcccat tgttgcaagg aattcattca atacccctcc gtcaacattc aaaaatacat
    12241 aagtaatcga aacaatgatt acccaagaca tgaagtgagg gatgtaaatg atcgtttgaa
    12301 tcccgttttt caatcgtttg ttcgttactt catttaatag taaagacaaa atgattggca
    12361 tcggaaaaaa cacgaagata tttaatccga acaagatcaa cgtatttctt aataatgtaa
    12421 agaatgtcgg ttctgtaaac aaacgaatga aatgtttgaa accgacccaa ggacttccca
    12481 ttattcctaa aaatggttga taatcttgaa aagcaataac gagtcccccc ataggaagat
    12541 acctaaaaat caagaaataa aggactcccg gcagaatcat cacatataaa aatttattcg
    12601 ttttgattcc tgcaattatt ttttttcgtc ttctgagctt ttcttctttt ttcgatagcg
    12661 tcagttgaaa tggttcttgt ttctttacac tttccataag cccctcctta gttttgtgaa
    12721 tgtttgctgc ttatgacttc atgatagata actatctgaa ttacgtatat aatcattttt
    12781 accgtaagta aaaacgctta catagcaatg atttttgtgc tatacaaaac gataaagtgt
    12841 attttttcac aaataaacta cgcatagcaa accttctatt tttttctatc tgacacttag
    12901 cataggaaat gattatatac gtaatctttc accgatgata aattgaacat atagaaacag
    12961 aaacatttaa tttattttga ggaggagcct atctatatga aaaagaaagt ctctatagga
    13021 gaaagaagtt ttacactttt caacaatacg tttctaattt tgctagcttt gatctgtatc
    13081 gttccttttt taaatatcat cgctacatct tttgcttcta cacaagaagt cgtcgcgaaa
    13141 aagtttatcc tttttcctac taccttttct ttagacgcgt atcgctacat tctttctacg
    13201 ccgaccattt ttagagcact tgctgtttcg attggtgtta caggtgtcgg aacgatcgtc
    13261 agtatgtgtg caacttcact gatggcctac ggtctttcca gaaagtattt gtttggtcga
    13321 ggtttcgtca actttcttgt tgtattttct atgttgttca gcggggggat gattccaacg
    13381 ttcttagttg ttcgttctct agggctggtc aattcttact gggccatgat cctgcctgtg
    13441 gctgttaatg caatgaatat gatcattatg agaaactttt tccaagcctt gccggatagt
    13501 ttggaagaat cagcaaaaat ggatggttgc acagattttg gtgtattttt caaaattatg
    13561 ctgccattag cgttaccgtc tattgctact atctcgcttt tttatgctgt tacttactgg
    13621 aacacttata tgactgctat tttgtatatc aacgattcat ctaaatggcc gatacagatt
    13681 cttttacgcc aaatcgtcat tgtatcaagt gggatgcaag cagaaagtag tgccgttgat
    13741 gtgattccac cagcacaaac agttaaaatg gctgttatcg ttattgcaac agtgcctatg
    13801 ctgattgttt atccttttgt tcagaaatat tttgtcaaag gagcactcgt aggttctgtc
    13861 aaagggtgat tatgtactga acgcaaaatg gtagttgtgt cagaagtggt cagcttcaag
    13921 aaataagaag gcatttacga aaattgcttc ttaaattttt gtgaaatttc ggcttatttt
    13981 tcgaagaatc tactcctgga acaccgtgta tcagaggttg tgaaagagac gttcagcctt
    14041 gagcgatgat aaaaactctc acaaacattg cgacgagtaa cccacaggag caataagaag
    14101 cgattttgaa aaatagcttc ccatatttca caaaattatg ccttattgtc gatagcctgt
    14161 ctcttgaacg ccatttattc gtaaaaaaga actgtgacaa gacttttgtc acagttcttt
    14221 ttgcgtgctt agccttgaaa tttaactatc cataaataca aatactaggg ttattctaac
    14281 ttcttttatt gcttgaatca gtccacaaga aaaacagcct cttgattcac aaatattttt
    14341 ttcgatattc tcccggcgta atcgattcct tctttttaaa gaaacgaata aaattttgcg
    14401 gatttcgata ttgcaatctt tctgcaatct cttttacgga gaggttagtc tctttcaacc
    14461 atttttttgc tacctctaaa cgatgatttt gaacaaaatc accaaatttt tctcctgttt
    14521 cctttttaaa tacattactt aaatagttag ggttataatg caacctatca gcgatcacat
    14581 ctaaagacag atcacgatca tattctgtgt agacgatatg cataatattc tccgacaaat
    14641 ttttaaattc atgatcagtg attgcttgag agcattctgt gattggcaga actaattgtt
    14701 ctaaaacaag tttttctagt tctttgggat gataattgtt cagtgctttt acataaattt
    14761 gtttcatgtt atcaaagttt tttgaatcta ctcccaataa ctgtcctaat tgaacgagtt
    14821 cattgatcaa gcggaccgaa actacttccc gactcaaagg atttttattt tgtgcaaaca
    14881 atgcatcaat caattcatgt actaaattct tcacttcctg atttccagaa cgaatggctt
    14941 caaacaatcg attttgttct tctatcggat atctaataag tgttttttca tgagcagcag
    15001 gaacgatttc ctgataaaaa ataattgaac ttggaccagt atttacacga taatacaggg
    15061 cttcttttgc ccgatcaacc gattgcttgc tttcattcag cgtttcgaac cggttactga
    15121 tgcctatact gatagttaaa ttcaagtatt cttttacacg tttttgaatg agtcggcaat
    15181 actccatgac ttttttgctt gcttctgcat catctttatc cattcgataa atagtggctt
    15241 gcatttcttc attcaaaacg atcggcagca ttcgattttc ctttggcaca atttccgtga
    15301 tcatgttgtt gatcgctaaa agtagcaaat cacgctcccc accgtgacta tcttctaaca
    15361 gatcgatttg aaccaaagct gtataatagc agtcattttg agaactataa ccaaattgct
    15421 gcaaccgctg atctagttct cgcctttcta ctcgatttct aaataaacta agtacaaata
    15481 gcgtttccag ctgtggcttt tgggtaacta agtgtgcact taacgcttct ttttctccta
    15541 tgatccgact gactgattga gcgaccaaat ctagttcttt cccactgaat ccttttgatt
    15601 taagattcag acgttcctgt atttgagaaa tcggacgggc aaatctgtcc gaaaataagt
    15661 aggaaagaag accaatgagt cctaccagtg ttaaagacac tgcaactagc ccgattctca
    15721 gattgcggat cattgcgcca attgcactag agtcaacttc taatctgtaa atccaattat
    15781 tataatctga tttcagtact atatacgttt tatctttttc tcttatcact ttcagctgat
    15841 tgtaatctag atccacgtcc gttaatacag ttggttgctc tttatttttt actgtctgag
    15901 tagtaaaaag gttgccaact tcgttttcaa tagaaagtat gtcttctcca ctttcaccaa
    15961 caacttgttc tattgtatgt ttctctatcg tagcaattcc caatgcatat ttttcttttt
    16021 caaacattgg caaagaaatc accatttcga ttcctttgtt tttcttttgc caaaataaat
    16081 tatttttgtt atttatgtac ttctgttgat acttttcaat ttcctcttcg gtcagttgac
    16141 tcaacgatcc attgattact ccccatttac cagccaaact gactaaatca taattattat
    16201 catccataac aatcgtttct ataaaattta gttctttcct gatttcagaa taagtggagt
    16261 aatctgtata tgtcagcgga tgcttgaata tgtccgagaa atttttggtc gaaccatacg
    16321 tagaaaacga atattcaatc gttctaattt tccattcaac gttagtttta acttgcctga
    16381 gcaaattctt tttgtctgtt aaataactcg attcgatcga ttcataggta ttctggtaaa
    16441 tgaataaact aaatcctatg acaagtacgg ctcctaataa aaacatcggt aaaaatattt
    16501 tataaaatac tgtacttttc ttcattttct tcctctcctc cttttaaaat aaaaggcttt
    16561 catttttctg taaaattgat actcatctaa ttaaatgagc attccccgct ttcaagaata
    16621 gcataattaa aaataaaaat ccaagtaatt tttaacgtgt gcaatctttt catatcaaag
    16681 atctattgac ttatcgtagt tatcgtaata tgatgaatgc gttaacaaaa agtgaaaaag
    16741 taaggaaaat gatggaatgg taacgatcaa agatgtcgct aaagcagctg gcgtttctca
    16801 tacgacagtt tctcgtgctt tgaataacag cagtttaata aaaaatgaaa caaaacaaaa
    16861 aatacgacaa ctagctaagg aaatgaatta ttcgccaaac tatagtgcaa aaggtttagt
    16921 gaatcaaaaa aaatatctaa tcggactatt tttctcaagt ttgcataaag gaacttcatc
    16981 aaatttccta gcagattcca ttgcaggcat ccattcagta ttagacacaa attacagttt
    17041 atctgttgaa agtatcgatg ttgttgattt aacagaaatc aacctgcaac gctacgatgg
    17101 actgatcgtt atgagtcaaa gtgatgatga tcagcctttc atcgactatc tcaaagaaaa
    17161 agaatttcct ttcgttgttg tcaatcgtca catcaatgat ccagcggtta tcaacgtcac
    17221 tgccgatgat gcggccggtg ttatgcaggc agtagattac ggtattggat gcggacatac
    17281 gaagatcgct tatattggcg gaaagaaaaa ttttcgttcg acaaaagaac gggaaaaagg
    17341 ggtattgaaa agtctaaaaa aagcgagcat ccctgtcaat ccagcctatt ttttaacagg
    17401 agattacagc ttagaaagcg gcctccaaaa catggaatac ctattatcac tttctgacct
    17461 gcctactctg gttttttgcg gcaacgacga catagcgatt ggtgcattga gagctgtaca
    17521 tttagctggc ctccgtgttc cccagaacat ttctctgatc gggttcgatg attctccagt
    17581 cgtctcctat ctcaatccgc cgcttaccac cgtacgtaag cctttgaaag aaatgtgtca
    17641 agaaggaaca aagcttttac tagatttgat cgaaggcagg acgatcgaac aatctaaaat
    17701 tgagctctcc accttgttga tccttcgtga gtcgatcaat tacttgtaaa ctacaagtaa
    17761 ttttatataa aaatgttaac gtgtgcataa cacagaaagg aagtttatat gaaaaaactg
    17821 acaaaagaat tagtgaaaaa aaacgaagca cctgtaaaag ttcttcagtt tggtgaaggc
    17881 aattttatgc gtggatttat tgattggcag attcagcagc tgaataacca acaattgttt
    17941 aaaggtaatg ttgcaatcat tcaaccttta gaacaaggtc ttggaaatat gatgaaccag
    18001 caagataatt tatatacggt cattttacaa ggactcagca atggcgaaat cgttgatact
    18061 tccgagatta tcaccgtagt agaacaaatg atcaatcctt atgatgaatg ggatgcttat
    18121 ctatctctag cagaaaatga cgacttggcc tatatcgtct ccaatacaac agaggcagga
    18181 atccaattcg tggagactga taatttaaca gatactcccc cacagagcct cccgggcaaa
    18241 ctgacagcct ttttataccg acgtttcaaa ttaaacaaag ctggctttac catcattcct
    18301 tgcgaactga ttgacagaaa cggcgaacgt ctaaaagaaa tcattttacg ctacgcagaa
    18361 aaatgggatt tagaacctgc ttttgttgag tggttagaaa aagaaaatgt tttctgttgt
    18421 agtttagttg atcgcatcgt cccaggttat cctcgcgaag atgcaggtgt tttcaatcaa
    18481 cggcataact atgaagatca attgatggtc aaagcagaac cattcatgct ttgggtgatc
    18541 gaagggccta aagaattgga gaatcaacta ccattcaaac aggctggctt gaatgtcatc
    18601 atcacagatg atatgactcc ttatcgtgaa cgtaaggtgc atttgcttaa tggaccgcat
    18661 acagcgatgg tccctcttgc tttgttggcg gaattggaaa ctgtagaaga agtaatgaaa
    18721 gatcctttat tctccgcata tgttgatcag ttattcaatc tagaattgat tcctatgctt
    18781 tctttaccta aagatgaatt agccatttat gctgaccaaa tcaaagaacg tttcttgaat
    18841 ccttttgccc atcacaaatt agaagcgatt tcattaaata gtgtttcaaa gttcagcacc
    18901 cgtctgcttc cagtgttcaa gaagtatatc gaagaacaaa atcaggtacc accgttaatc
    18961 actgtttcac tagctgcgct acttcttatg tatcgaggcg atcaagtaaa gccacatgat
    19021 gatgaaaaga cgataagcga atttactgat gcttggtctg ataatggaac agctattcct
    19081 cgattattgc aaaacgctgc gctttgggga gaagatttgt ctcaaatacc gaatgtgaca
    19141 gatacagttc aagaaatagc tgcaaagatc gatcaaaaag gtgtccggtc agtattacaa
    19201 gaaatgaaag gaacgatggt ccatgcttga aaccctgcaa ccaacaatga aattaaatcg
    19261 acgagacagt gtggtcgtcg cattaacccc tattccaaaa caaacacaac tgacgattga
    19321 tcagcaaacg atcaccactc ttgaagatat cccgcaaggt cataagattg cattgggtga
    19381 tttgaaagct ggagacaacg tgatcaaata tggctatcca atcggtcatg tgacgacaga
    19441 cgtaactgcc ggtcaatggc tgcacactca taatgtaaaa accaatcttt ctggtgaatt
    19501 ggattatcga tatgaaaaag atgtccatcc aagtcattac ctttttgaga accgaacatt
    19561 tcaaggttac ttgcgaaaaa atggaaaagt agggattcgt aatgatttgt ttttagtacc
    19621 taccgttggg tgtgtgaatg gcattgctga actgatcgtc aaacagttca aggaaaaaca
    19681 tccagatctg ggacagtttg accatattac gattttaaag catccttatg gctgttcgca
    19741 gcttggaaaa gatcatcaaa atactcgaga aattttagcg gacgcagtaa atcatcccaa
    19801 tgccggagga gttttagtat ttggattagg ttgtgagaac aatacagttc ctgaattcaa
    19861 aaaaatttta ggtgaatatg atgaagaacg agtgaaattt ctagtggctc aagatgttta
    19921 cgatgagatc gaacaaggtg tggctctatt agaagagctt ttaacagcgg cggaaaatga
    19981 ccagcgcata tcagtcccgc tctctaagtt gaatgtcggt ttgaaatgcg gcggttctga
    20041 tggtctatcg ggaataactg ctaatccttt acttggtgca ttttctgact acttgattgc
    20101 tcaaggaggc agcacgatct taacagaagt gccagaaatg tttggcgcag aacaagtatt
    20161 gatggcacga gcggaaaatg aagaagtttt tgactcaatc gttgacttga tcaatgactt
    20221 caaacagtat ttcttgtctt acggggaacc agtttacgaa aacccatctc ctggtaataa
    20281 agcaggtggg atcactacat tagaagataa gtctttaggc tgcacgcaaa aatcaggaag
    20341 ctctcctgtc gttgatgtgc tacaatatgg cgaaaaaatc agaaaaccag ggcttagttt
    20401 attgcaagct cccgggaatg atcttgtagc tgcctctgct cttgcttcat ccgattgcca
    20461 gttggtcttg tttaccacag gaagagggac tccgtttggc tcatatgtcc caacggtcaa
    20521 ggtatctacg aatacaactc tttttgaacg caaaggtcat tggatggatt ttaatgcagg
    20581 cgtcttatta gaacaaccaa tggaagtaat cttagaatcg tttgttcaaa aaatcatcgc
    20641 tgtagctagt ggagaagaaa cgaaaaatga acaaaatgac gttcgagaaa ttgccatttt
    20701 taaaaacgga gtaacacttt gattttcata aaacagtata aaaagaaaga aggaagattt
    20761 atgtttttat ctgatgattt tttattaaaa gacgattggg cgaaaaagct ttaccattcc
    20821 tacgcaaaaa acatgcccat tattgattat cactgtcact tagatccaaa agaaatttac
    20881 gaaaacaaca atttcagcaa tctgacagaa gcttggttag gaggcgatca ttacaaatgg
    20941 cgcttgatgc gagcttgcgg cgtgcctgaa gaaaaaatca caggaaatgc ttctgatttt
    21001 gaaaaatttt tagcttggtg tcaaaccgta ccaaaaatta tcggaaatcc gctttacagt
    21061 tggtcacatc ttgaattgaa gcgtttcttc ggtattgact tgttgataaa tgaagaaaat
    21121 gcagagaaaa tttggacaca agcaaatgaa attttggcag ctcctgattt tcgccgtcgg
    21181 gaaatcgcaa aaaaatcgaa tgtagaagtg atttgtacaa cagatgatcc cgcagacgac
    21241 ttgcactatc atcagttgct tgctcatgaa gaaccagatt tgaaggttct accttcattc
    21301 cgtccagata aagcactcaa tattgaaaaa gaaggattta atgcatgggt cacactactg
    21361 gaagaagcag ctgaccaaac gatcaccacc tatcaagaac tgatagaagt cttagggcag
    21421 cggatagaat acttccatca acatggatgt cgtgtttccg accacgcttt agacatctta
    21481 cgctataaag aagcggatga aacagtatta gaagagattt ttcaaaaagc acgagctaac
    21541 aaactattat cttcagatga aatcgatgct tatcgtacag aaacactgat acatttgacc
    21601 cacttctatc attctcataa ttggacgatg cagctgcaca tccatgctta tcgtaactgt
    21661 aatacacaaa tgttggaaaa attagggccc gataccggct atgatggaat gaatgatctt
    21721 tctttgacta ttcctctgca aaaattattg aaccgtgcag aagaaacgga tcagttgcca
    21781 aagacgatcc tttattcatt gaatccgaat gactatcctg cgattttagc tttgatgggc
    21841 tgtttccaaa aagaacagaa tggaaaactg cagttaggtt ctggatggtg gtacaacgat
    21901 actcgagctg gtatgcgcga tcaattgaca caatttgctg atggtagtgc tttggggaat
    21961 tttgtcggca tgctgacaga ttcaagaagt ttcctgtcct acactagaca tgaatatttc
    22021 cgtcgtgttc tttgtgaatt tttaggcgaa atggtcatgc gaggagaagc acctgaagat
    22081 gaacaacttt taggaagtct agttcaagcg attagttata caaatgctaa aaattacttt
    22141 ggcttttttg cctaattgat taatagtaac agtaacgaat caaaaacagg atagtgaaat
    22201 aacggaatcc gtttatccac gttttagaga agaagacgga ccaaatttgc tccgtcttct
    22261 tctcttgctg ttctgcttca ttgcctataa acctttatga aagaagagat aacatgccta
    22321 ctatccatat tgccggtgat tctactgcgg cagaaaaaga tgaaaacaag cgtcccgaga
    22381 caggttgggg agaaaaaatc tctgcttatt tttcacccga gatccagatc aataaccaag
    22441 caaagaatgg acgcagcacc aaatcattta tccaggaagg aaggttagcc cgtctagctt
    22501 cacagtttca gccgaatgat ttcttatttg tacaatttgg acataacgac cagaaaatcc
    22561 aagaagaaag aggtacgctt cccttcggta gctatttaaa aaatctgcag atattttttg
    22621 aatgcgcaaa aaaagcgaac gtccaaatgg tcttactgac atctgtgacg cgacgagatt
    22681 acttagaaaa cggaacattg aatccagata tacttggaga ctatccaaaa gcgatgcgtg
    22741 catttgctga gaaacaccat attcctcttt tagatgtgtt ctcaagatca caagaattgt
    22801 tccagacctt ctcaaaagaa gagacaaaaa aattctacct gcatcttata cctgatactt
    22861 gtaaaaatta cccagaagga ctaaaagaca atacccactt tagtccagaa ggcgctgata
    22921 aagttgcccg aataattgtt gaattgataa aagaatcccg tctgcctctt gctaattatt
    22981 tacaaaaagg agtttgagat gtttccatta aatttaggca tccgtgccca cgatttagat
    23041 gttcgaaatc gtgaagaaat cgttcaaaaa gtatcacagc ttcacttgtc ccatatccaa
    23101 tttgcaccac aaaaagcatt tcctgaacaa cttaaattaa ctgacatgac acttgggagt
    23161 gctgcctatt ttggtgaata cttccaaaaa aacaatatcg aactttccat tttaggatgc
    23221 tatatcaacc tatccagcaa aaatcaagaa gttcgtgcac aagcattggc aactttcaaa
    23281 cagcaaattg ctctgtgctc tgcctatcaa gccaagatga ttgctacgga aaccggtagt
    23341 gtctctgtcg gttacacaaa agaaaatttt actgaagaag cttatcagat cgccagaaat
    23401 tctatcattg aattagtcgc tcatgcagaa aattttggcg taactgttgc tgttgaagca
    23461 gggatcaatc atccaatcta taactatcag ttagctaaac gtttaatcga tgaagtagct
    23521 tcaccaaatt taaaaatcat tttagattgt gcgaatttga tgcataaaga aaatcatgct
    23581 gatcaggaaa aagtgatccg aggtgccttg gacaatttac atggttatat tacagcgctt
    23641 catctcaaag actatatcat ggaaaatgga aagatccgca tggtacctgt tggcaagggc
    23701 tgtttagatt tccgcccggt actgcattat atcaaatatg agcgtcctct aatgtacgcc
    23761 actcttgaag ctactcctga gatttatgtt cctgaagcaa tagaacactt gcagtttatt
    23821 taccaagatt gttaaggaga agattatgga ggaacgaata cagaaaagtt atcaaaaata
    23881 tcatgcaaat gcttttcaga cattgctgca tctttatcag ggaaattatt ggcgttttgc
    23941 catgtcagga ctctttttta ttatcaaaca tcttccatct tggttaatgc cgatcgctat
    24001 tgccaatgta atcaatgcag cttcatcgaa agattcttct cagttgcagg ccatttattt
    24061 caacgctttt ttgatggctt gtctgattgg tcaaaatata ttgacgaatt attttcatgt
    24121 aaaataccat agcacttcga tccgtcaagt agaagctgga ctaagagcga cccttatcaa
    24181 gaaaatccaa gaactatcta ttccttatca aaaagaattg caatctggac gaatccagtc
    24241 aaagatcatc cgtgatgtag aagctatcca aacgttgtcc tctcaagtat ttgtcagcgg
    24301 attaaatatc gctgtcaatt tattcgtagc tttaggtatc acgctttata aaagtcctgt
    24361 tgttttcatg tttttcttat tgactgttcc tctcgccgca ttcgttactc tctttttccg
    24421 taaaaggatc agacagtcca actcagactt caggcaagaa atggaagaga cttctgcaaa
    24481 agtgatcgaa gtgatcgaat tagcaccgat tgctcgagct catgggttag aaaaacacga
    24541 aacaactcgc ttaaataagc attttactca aatctatgaa aaaggattcc acttagatat
    24601 cctgcaatct gttttcggat cagtcagctg ggctagtttt cagatatttc agctgatttg
    24661 tttgattttc actgggatat tagctttaca agggcgcatc caacccggcg atgtcgtgat
    24721 gtatcagacg tatttcacga cgatcgtcaa ttccgtttct ggaatgatca cgctgattcc
    24781 tacgatttca aaaggcatgg aatctatcac ttctattggc gaactgttga ttgctggcga
    24841 tgtagaaact tatcacggta agaaaaaaat ccaacatttg gatggaaatt ttcgttttga
    24901 ggatgtttct tttctttatc cggactcact caaaccgatt atcgatcatt tttctctaga
    24961 ggtgaaagct ggagaaacaa ttgcttttgt tgggccatct ggctctggaa aatctacact
    25021 ccttaactta ttgattggat ttatccagcc gacttcagga aaaatttttc tagatgatca
    25081 gcctttggat gaactggata tgcgaagtgt gcgaaattat ttagccgttg ttcctcaaac
    25141 cactttgttg ttctccgctt cgatcaaaga gaacatcact tatggcttga aaaacgtatc
    25201 aaaagaacgt ttagatgaag tcatcgaagc tgcgcaactt tcttcactga ttgaccagct
    25261 tcctgatggt ctggatacct tagtaggcga acatggcaac aagctgtctg gtggccaaaa
    25321 gcaacgtatc tccattgcac gagcgttgat acgtcagcca aaaatcattt tgctagatga
    25381 agcaacctca gctttagata accagtcaga gaaaaaaatc cagcaagccc tgaattacct
    25441 gacagaaaca cccactactt ttattgtggc tcatcggctt tcaacgatca aagaggcaga
    25501 taaaatcgtt gtgatcgatg aaggaaaaat cgttgaaata ggtacttacg aaaaactgtt
    25561 agctcaaaaa ggctatttct accgcttgaa aaatgaatga caaaaaatag gagctatgac
    25621 aagacttttg tcgtagctcc tatttctgaa taaacggtgt tctcctgcca actcctcggt
    25681 cacaagtcac aatagtcatc aaacatgaga agctgttttt tgaaattgtt ccttgtaact
    25741 cagagttaaa cggtaggctc acgacctctt ggtaaatggt gttcaagaga cagcctatcg
    25801 gcaataagaa gcaattttgt catcgctcaa ggccgaacgt ctctttcaca ccctctgttg
    25861 tttcataaaa tagttttatt cgctgtaaag gtcgcgaact ggtgtcatga tgatacggtt
    25921 caagtcattg atgattgtac tgaagccttg ctctttttgc atcaagtcat tgattacttc
    25981 ttctgattgg attttcacag ccatttcttg tgctttcatt gcatcttcgt cgctgaactc
    26041 ttgtccactc atttgttttt cttgtaattc gatttgcatc gcttggaagt ctttgaacag
    26101 tttgtgtgct tcttcgtttg cttgtacttt ctcgtacgct tctttcaacg ctttgaattc
    26161 tggtaattca cggatctcac gttcgatttg attcgcgcta tcgtagatgt ttgacatgtt
    26221 atgccctctt ttcttttagt agttactctc tattgtaaca tggggttatt cgttttcaaa
    26281 ggggctactc tccttgatat ttccgccaga ggtctttagc cgcttcacca attccctgaa
    26341 cgccttcttt caccatatcg ccgaagtttt tcagcccttc ttttgcttgt tcaccatatt
    26401 gattcaagtt atcttgccaa tttccagact ctccattaga atttcctgtg tttccaactt
    26461 gatctgcagc taccacttgt cctcctgtgg cataggcatc tgctacacta aatcctgttt
    26521 ttgaagtata cggaaggatg ccttctgccg ctgatttgaa gactttcccg acaacatttc
    26581 cacttgtccc ttccaaatag tgcagcttgc tggattcttc gtaacccatc catgtcgaaa
    26641 tcactacgtc tggcgtgtaa ccgatcatcc attggtcatt tgcttttgtg gcatcgaaat
    26701 tagtttctgt ggttcctgtt ttccctgcta tcgtataacc tgcaggattc gcagcagccg
    26761 ctgttccatt tgaaaatgta ccaagaagca tgctggtcat ttcatcggct gtttcctttg
    26821 atatgacctg ttttggtttt ttgtttgtgt tatcgacgat caccgctcca gtcgaatcaa
    26881 cgattttcgt gataagatgc ggcgtataca tctgaccatc attggcaaat gcactgtaag
    26941 cacccgccat gatcaatgga gatactcctt tttccagtcc accaagggct aagccataat
    27001 acttatcaga ttttgctaaa ggcaggccga attcttctgc tttatcatat cctttttgta
    27061 aaccgatctc atgcaaaagc catacagtag gcaaattcaa actttgtgca acagcttgat
    27121 acatcggtac ttctccttgg tatgtcccgt caaaattatg cgcatcgtaa taactttgcg
    27181 gttcgtcttt caagatactg gaaggcttgt atccagcttc cagtgctggt gtatatacgc
    27241 ttaatggttt gatcgttgat cctggtgaac gacgcatctg tgtagcaaag ctgaatcctc
    27301 taaagacatg atctcctctt cgtcctacta gtgcttgtac accacctgta ttcggatcta
    27361 aagctactga agcagattgg accatggcac catctgaagc atttgctggg aataatgcac
    27421 tgttttgata agtagcttct aattgctttt gataatcctg atctaaagct gtatagattt
    27481 tatagccttt attcatgaca tcttcttcat caagaccata ctcgttaact gcttcatcga
    27541 tcactgcatc aaaataataa ggataacgat aaccagaatc tgaattatcg tatgtgtcat
    27601 taagatacgc gctgatatct gtctggcttt ctgcttgttc ttgttctttt gtgatttttc
    27661 cgttttcttc cattacactt agtactgtgt ttttccgatc attcgcattt tttgggtgat
    27721 cgatcggatt ataaatacca ggacctttca gcattcctgc caaagtagca gcctcagaca
    27781 gcgtaacatt cgttgcatct actccaaaat actttctgga agcgtcttgg acaccccata
    27841 ccccgttgcc aaaataggca ttgttaagat acatggtcaa gatttcttct ttgctgtact
    27901 tcttttcgat ctcgattgcc aaaaataatt ccttcgcttt tcgatcaaac gtttggtcta
    27961 atgtcagata cgcatttttg gcaagctgct gagtgatcgt acttcctccg ccccctgatg
    28021 ttccgccaga caagaccatt cggactgctg cacgggcaat ccctttgata tcaaacccgt
    28081 gatgctcgta aaaattccta tcttctgtcg agattactgc atcttggata tagggagaaa
    28141 tcgcgtttaa ttctacatat gttccttttt gggcgtacag tgtgccagct tcttcgtttt
    28201 tcttatcata aatgactgtc gattggctta atcctgattt taacgtttcg acatttgctt
    28261 gttttgctaa aataaagagg tagatacttg tcactaagac acagatcaaa ccaatcagca
    28321 aaatcaattt gttcacatga tattttttcc agatgcgctt tcgccatcga tggaattgtc
    28381 cgagatatgg tctgatccaa cgccaaaaac tgatcaaagc agcttttatc ttttctaaaa
    28441 tgctttgaaa atccatttgc ttcttctcct gtctatttga ttcaatcgtc ttattttacc
    28501 atagtgaaag agtgttttct tcctgcttaa gttgtagttc aatatgtttt tttacgttag
    28561 tgcaagtaga gtaacataag aatgaacaga aaaatgtaag ttttttctta aatatgtttt
    28621 aaaaatggta caattagtct gcgattgcac tgaaaggagt acacttgtga aaatcacgat
    28681 cactttacct atgaaccatc ctgaaacaac aattcgcgac ttgcttgaaa aggaatggtt
    28741 agttcctaga aaggttcgac actttttgcg tatacgtaaa aacgtttgga tcaatcaaca
    28801 acctgctttg ttccatcatg aagtaaaggg caatgaccag atcacattgt tattcgaaga
    28861 aacagattat aattatcaag aagttcaatt aggagatgcg aagcacatcc gtgttttata
    28921 tgaagatgag catttgatta tagtggataa gcctgcagga atgaagactc atccaaatga
    28981 accgacagaa aacagtacct tactcaatca tttagctgct tatctcgaca ctaaacagca
    29041 acgaccttac gttgtccacc gcttggacaa agaaacaagc ggtgccgttt tatttgcaaa
    29101 aaatccattt gtcttgccga tacttggaag aatgttggaa caaaaaatga tctatcggag
    29161 atatcaagcc atcgtatggg gagaaattgc caaagatatg accatcacca ataaaattgg
    29221 acgagatcgc catgatcgga gaaaaagggt cgtagatgat aaaaatggtc agagcgcctt
    29281 gacacatgtc actgtcaaca aagtactttc tgaacaaaca caagtctatt gtgttttgga
    29341 aaccggacgt acacaccaga tcagagttca tttagctcat aagggacatc cgatcgttgg
    29401 agaccctctc taccaaaata gaacagctga acgactgatg ctccatgcat tagaactgca
    29461 catgatccat ccatttactc aagaaaaaat agtcggtaaa gctttacctg gattatggga
    29521 atagaaataa aaaagaacag agtcttatca gcagataatg ataagactct gttctttttg
    29581 tgtaatttgt ctgcttactt ttgatgttcg tgttctgcta acagcaaact accgataatc
    29641 ccactttcat tcggcagtcc ccaacgaacg atatattcac ttacttgagg tgtttccata
    29701 taaccaccca tcaattcaac aaattgctga cggatttttt gaaggagatg atcctgattc
    29761 attaccccac cgcctaaaat aatcttttca ggtgctaacg tcaacgtata gttgatcagt
    29821 gcttgagcaa tataataagc ctgaatagcc caaacaggat gatcttgcgg cagatcttgc
    29881 cctttgatcc ctgtgcgtgc ctcaatagac ggtcctgctg ctaatccttc taaacaatct
    29941 ttatgataag gacaagtacc ttcataatgg tcatctggat ggcgtttcac ccaaatatgt
    30001 cccatttcag gatgtgcgat ccctgaaaaa atttcttcat tgatcacgac acctgcaccg
    30061 attcctgttc ccacggtaag gtaaatacaa ctattttttc cttgtgctgc tccttgcttc
    30121 aattccccat atgcagcagc attgacatcc gttgtccaag cagcaggaat attgtagcgt
    30181 tccttgattg tgccaagaaa atcgaattgt gcccaccctt ttttgggagt tgccaaaacg
    30241 tatccgtaat tatcttttgt tttgtcgata ccaatcgggc cgaatgagcc aatcccgatt
    30301 cccttcaatt cataacgatc aaaaaattca aatacctgat ccaatgtttc ttctggtgtt
    30361 gtggtaggga tacttaattt tttcttaatt gtcaaattaa gttagacaga tcatcaatac
    30421 ttacgtaact caaaaatact tccaaaggtg ttcgataatc cagtgatttt ctaggaatat
    30481 tatttcgttt agatgcgaca gattgaataa aaaattcatc tactgaattg aaatccatag
    30541 atttcaataa accatctcta cgtaacaatc cgttagaatt ctcgtttagg cctctttgag
    30601 acggcgttcc tggatcagcg aaataaatgg caatatcatt gacattactg atctgtttcc
    30661 aatttgaaaa ttcctttcca caatcaaaag tgatggattt gaataggttt ttcggtacag
    30721 attcaaacca ttgatttaat tttttttcaa tatcaatcgc ttgtctacca cacggtttca
    30781 atgtgatgat aacttttgat aatcgttcaa ctaaggtaat tacagcactt ttatgtttca
    30841 gacctacgat agtgtcacct tcaaggtgac caaactcatt tgagaattgg ctataatcct
    30901 tttcacgttc atgaatagag cggcggaaag tttgttttcc acgtctttct tgatgtccat
    30961 tcggtttacg cttgcctttc atcggtaagt cagaagaatc aaatagcccc tttttgaaca
    31021 tacgataaat ggtacgagca gaacaactaa taggaaacgc tgcacgacca acaatcacat
    31081 cgggtgtcca tccttgaaca acctttcttt gaatatattc tgattgttcc tcgggtaaaa
    31141 caagcggacg tctaccacag tttgatttgt ttttcttata ctgttgataa tattctaagg
    31201 ctgattttcc ttgcttgaag aacagatata ctttatggat cgtttgtctt gaacgattca
    31261 agcaatgggc
//
LOCUS       DAZKLA010000023        31204 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000023 DAZKLA010000000
VERSION     DAZKLA010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.023"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..371
                     /locus_tag="ACG85N_000853"
     CDS             3..371
                     /locus_tag="ACG85N_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFQ0223647.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="ACG85N_000854"
     CDS             551..1492
                     /locus_tag="ACG85N_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFQ0223648.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1561..2292
                     /locus_tag="ACG85N_000855"
     CDS             1561..2292
                     /locus_tag="ACG85N_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0223649.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="ACG85N_000856"
     CDS             2436..4616
                     /locus_tag="ACG85N_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFQ0223650.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="ACG85N_000857"
     CDS             4636..5118
                     /locus_tag="ACG85N_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFQ0223651.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="ACG85N_000858"
     CDS             5134..5895
                     /locus_tag="ACG85N_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0223652.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /gene="murC"
                     /locus_tag="ACG85N_000859"
     CDS             6026..7360
                     /gene="murC"
                     /locus_tag="ACG85N_000859"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFQ0223653.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="ACG85N_000860"
     CDS             7667..8152
                     /locus_tag="ACG85N_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFQ0223654.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="ACG85N_000861"
     CDS             8145..8828
                     /locus_tag="ACG85N_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFQ0223655.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="ACG85N_000862"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="ACG85N_000862"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFQ0223656.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="ACG85N_000863"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="ACG85N_000863"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFQ0223657.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..13073
                     /gene="coaE"
                     /locus_tag="ACG85N_000864"
     CDS             12444..13073
                     /gene="coaE"
                     /locus_tag="ACG85N_000864"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFQ0223658.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13125..13457
                     /locus_tag="ACG85N_000865"
     CDS             13125..13457
                     /locus_tag="ACG85N_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0223659.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13769..14260
                     /gene="nrdR"
                     /locus_tag="ACG85N_000866"
     CDS             13769..14260
                     /gene="nrdR"
                     /locus_tag="ACG85N_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFQ0223660.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14283..15656
                     /locus_tag="ACG85N_000867"
     CDS             14283..15656
                     /locus_tag="ACG85N_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0223661.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15656..16582
                     /gene="dnaI"
                     /locus_tag="ACG85N_000868"
     CDS             15656..16582
                     /gene="dnaI"
                     /locus_tag="ACG85N_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFQ0223662.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16711..17151
                     /locus_tag="ACG85N_000869"
     CDS             16711..17151
                     /locus_tag="ACG85N_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFQ0223663.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17295..17996
                     /locus_tag="ACG85N_000870"
     CDS             17295..17996
                     /locus_tag="ACG85N_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFQ0223664.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17993..19114
                     /locus_tag="ACG85N_000871"
     CDS             17993..19114
                     /locus_tag="ACG85N_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFQ0223665.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19129..20361
                     /gene="pepT"
                     /locus_tag="ACG85N_000872"
     CDS             19129..20361
                     /gene="pepT"
                     /locus_tag="ACG85N_000872"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFQ0223666.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20493..21401
                     /locus_tag="ACG85N_000873"
     CDS             20493..21401
                     /locus_tag="ACG85N_000873"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0223667.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21436..21708
                     /locus_tag="ACG85N_000874"
     CDS             21436..21708
                     /locus_tag="ACG85N_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFQ0223668.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21719..22765
                     /locus_tag="ACG85N_000875"
     CDS             21719..22765
                     /locus_tag="ACG85N_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFQ0223669.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23016..25625
                     /gene="clpB"
                     /locus_tag="ACG85N_000876"
     CDS             23016..25625
                     /gene="clpB"
                     /locus_tag="ACG85N_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFQ0223670.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25776..26462)
                     /locus_tag="ACG85N_000877"
     CDS             complement(25776..26462)
                     /locus_tag="ACG85N_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223671.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26440..26934)
                     /locus_tag="ACG85N_000878"
     CDS             complement(26440..26934)
                     /locus_tag="ACG85N_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0223672.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26954..28174)
                     /locus_tag="ACG85N_000879"
     CDS             complement(26954..28174)
                     /locus_tag="ACG85N_000879"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0223673.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28321..30156
                     /gene="lepA"
                     /locus_tag="ACG85N_000880"
     CDS             28321..30156
                     /gene="lepA"
                     /locus_tag="ACG85N_000880"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFQ0223674.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            30352..>31204
                     /locus_tag="ACG85N_000881"
     CDS             30352..>31204
                     /locus_tag="ACG85N_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0223675.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNR"
BASE COUNT        10539 a         5486 c         6702 g         8477 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13021 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13081 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13141 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13201 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13261 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13321 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13381 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13441 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13501 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13561 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13621 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    13681 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    13741 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    13801 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    13861 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    13921 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    13981 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14041 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14101 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14161 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14221 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14281 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14341 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14401 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14461 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14521 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14581 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    14641 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    14701 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    14761 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    14821 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    14881 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    14941 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15001 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15061 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15121 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15181 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15241 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15301 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15361 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15421 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15481 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15541 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15601 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    15661 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    15721 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    15781 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    15841 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    15901 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    15961 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16021 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16081 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16141 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16201 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16261 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16321 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16381 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16441 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16501 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16561 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16621 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    16681 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    16741 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    16801 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    16861 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    16921 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    16981 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17041 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17101 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17161 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17221 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17281 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17341 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17401 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17461 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17521 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17581 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    17641 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    17701 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    17761 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    17821 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    17881 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    17941 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18001 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18061 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18121 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18181 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18241 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18301 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18361 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18421 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18481 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18541 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18601 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    18661 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    18721 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    18781 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    18841 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    18901 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    18961 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19021 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19081 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19141 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19201 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19261 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19321 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19381 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19441 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19501 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19561 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19621 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    19681 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    19741 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    19801 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    19861 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    19921 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    19981 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20041 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20101 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20161 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20221 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20281 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20341 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20401 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20461 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20521 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20581 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    20641 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    20701 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    20761 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    20821 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    20881 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    20941 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21001 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21061 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21121 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21181 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21241 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21301 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21361 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21421 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21481 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21541 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21601 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    21661 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    21721 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    21781 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    21841 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    21901 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    21961 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22021 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22081 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22141 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22201 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22261 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22321 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22381 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22441 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22501 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22561 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22621 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    22681 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    22741 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    22801 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    22861 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaactatgtg
    22921 tatattttga ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata
    22981 agtgatctta cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga
    23041 cgcttcaaga agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata
    23101 tcgacattgc ccatgtatgg aaaatctttt tacaatctaa tcattttggt cgaaatttct
    23161 atacggatgc cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg
    23221 agtatccagt tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct
    23281 ttcgattatt gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa
    23341 cggaagtcgt tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga
    23401 ataaaaacgg attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag
    23461 aaagagtgac ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg
    23521 atttagtaca gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa
    23581 tccgtgatgt catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag
    23641 aacccggtgt agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag
    23701 atgtgcctga aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg
    23761 ctggagcaaa attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca
    23821 aaaaaagtga tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag
    23881 gaaaaacaga aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg
    23941 aattacattt gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca
    24001 aagcgttgga acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga
    24061 tcagtattct gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg
    24121 acaacgcatt agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac
    24181 cggacaaagc aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact
    24241 ctatgccaac ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag
    24301 ctgcactgaa aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag
    24361 aattagctga acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag
    24421 aagaagtcaa ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag
    24481 aagatgcaga aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac
    24541 ctcaattaga aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga
    24601 tggttcaaga gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta
    24661 tccccgtaac gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac
    24721 ttcataaacg agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc
    24781 gttcacgggc tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac
    24841 caactggtgt cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag
    24901 aagatcatat ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc
    24961 tagtcggagc tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg
    25021 tcagacgaaa tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg
    25081 tcttcaatat cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag
    25141 tagtagattt caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc
    25201 tggaaggcgt gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc
    25261 tattacgtgg aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta
    25321 caccattgag cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac
    25381 aacgtctgga acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg
    25441 ctgaaaatgc ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg
    25501 aagtagaaac accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag
    25561 taacgattac tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg
    25621 tctaatcttc cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc
    25681 tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt
    25741 cttgaagctg cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat
    25801 ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt
    25861 aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt
    25921 ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg
    25981 tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg
    26041 aataagagga acaaccaagg actggttttt gatatctcag catctttcat caagtaacca
    26101 atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc
    26161 caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag
    26221 aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg
    26281 acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct
    26341 tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca
    26401 ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc
    26461 atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt
    26521 caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc
    26581 ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa
    26641 gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc
    26701 gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc
    26761 agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc
    26821 ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg
    26881 gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac
    26941 atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct
    27001 ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag
    27061 cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc
    27121 ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac
    27181 cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa
    27241 caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt
    27301 cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa
    27361 tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat
    27421 ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc
    27481 agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct
    27541 catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac
    27601 catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta
    27661 atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa
    27721 aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag
    27781 gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta
    27841 gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac
    27901 ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga
    27961 tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg
    28021 aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt
    28081 ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta
    28141 attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga
    28201 taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt
    28261 aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct
    28321 atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct
    28381 catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg
    28441 acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga
    28501 atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat
    28561 atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc
    28621 cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa
    28681 acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat
    28741 aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc
    28801 ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat
    28861 attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta
    28921 aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg
    28981 atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca
    29041 tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg
    29101 gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg
    29161 gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg
    29221 aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg
    29281 gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct
    29341 caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc
    29401 caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt
    29461 taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct
    29521 gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca
    29581 aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg
    29641 atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt
    29701 gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat
    29761 gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa
    29821 aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca
    29881 atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca
    29941 gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg
    30001 gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa
    30061 gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc
    30121 gttctgaaaa tggatgagca agatagtgtg aaataactta taatcaatcg aaaaacctat
    30181 taaataaacc tttttgagta gatgcaacac tgaggattat ctgtataaaa gattttaaac
    30241 tgtcatttgg ttatcacggc gatactaaaa ttaacaaaat tggaatattt ttcttgaaga
    30301 tttttagctg tcagtatatg atgaaagcat ctcataggag gtgctttttt catggttgat
    30361 aattcaagtg gaagggttct taaatctcta agaaaagaga aaaaactatc tcaaaaaaag
    30421 ttagctgatt tagcaggtat ttcacaaagt actttagtta aatatgaaaa agggtcaaga
    30481 aaaatcccta aagacgtaga taatactcta agcaagattc tgaatataga aactctaata
    30541 aaagatgagg aagataagat tgagattctc atcggtaaac taattgcata cagagatatg
    30601 aataagttac ttaacaaaga gttagctgat aatataggaa ttagtgaagt attactcagt
    30661 tatgtgttaa atagaaagag aaatccgtca aaagaaatgc aaaaaaaaat cgatattttt
    30721 ctattaagta atgaaaaaga aattttgaag gagattaatc gtgatagtga gatatttagt
    30781 ttatcaaaag acgataaaat tgttatggga aaaagaatac gagaagtccg aaagaaccgg
    30841 gaagagacgc ttgaaaaatt tggtaaaaac tttaccatct atacaggcaa aaatgttata
    30901 agtagatggg aaaaaggtat taatattcca gatattgaaa aactaatgaa tattgcctat
    30961 cttggtaagg tcactgttcc ttaccttatg tacggagaag attataagaa tatacttcca
    31021 aaagatgaaa gagttagcga ttttaaaaag ataaattctt ttagtatggg attaaggatg
    31081 aggaaaatta ggaaagatta ttatctagaa agggaggagt ttggaaagct tttttctcct
    31141 tcaatttcaa aatggtccat agataggtat gagaatggta gagatatacc caatactaat
    31201 cgaa
//
LOCUS       DAZKLA010000024        31176 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000024 DAZKLA010000000
VERSION     DAZKLA010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.024"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..354
                     /locus_tag="ACG85N_000882"
     CDS             37..354
                     /locus_tag="ACG85N_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0223676.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            347..955
                     /locus_tag="ACG85N_000883"
     CDS             347..955
                     /locus_tag="ACG85N_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFQ0223677.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            1010..1801
                     /locus_tag="ACG85N_000884"
     CDS             1010..1801
                     /locus_tag="ACG85N_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFQ0223678.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            1939..2427
                     /locus_tag="ACG85N_000885"
     CDS             1939..2427
                     /locus_tag="ACG85N_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFQ0223679.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            2478..3350
                     /locus_tag="ACG85N_000886"
     CDS             2478..3350
                     /locus_tag="ACG85N_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0223680.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            3526..3888
                     /locus_tag="ACG85N_000887"
     CDS             3526..3888
                     /locus_tag="ACG85N_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0223681.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            3897..4649
                     /locus_tag="ACG85N_000888"
     CDS             3897..4649
                     /locus_tag="ACG85N_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0223682.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            4663..6465
                     /locus_tag="ACG85N_000889"
     CDS             4663..6465
                     /locus_tag="ACG85N_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0223683.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            6742..7782
                     /locus_tag="ACG85N_000890"
     CDS             6742..7782
                     /locus_tag="ACG85N_000890"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0223684.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(7834..9978)
                     /locus_tag="ACG85N_000891"
     CDS             complement(7834..9978)
                     /locus_tag="ACG85N_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0223685.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(10335..11708)
                     /locus_tag="ACG85N_000892"
     CDS             complement(10335..11708)
                     /locus_tag="ACG85N_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0223686.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(11708..12103)
                     /gene="rbsD"
                     /locus_tag="ACG85N_000893"
     CDS             complement(11708..12103)
                     /gene="rbsD"
                     /locus_tag="ACG85N_000893"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFQ0223687.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(12131..13048)
                     /gene="rbsK"
                     /locus_tag="ACG85N_000894"
     CDS             complement(12131..13048)
                     /gene="rbsK"
                     /locus_tag="ACG85N_000894"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFQ0223688.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(13156..14169)
                     /locus_tag="ACG85N_000895"
     CDS             complement(13156..14169)
                     /locus_tag="ACG85N_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFQ0223689.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLRLNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(14250..14849)
                     /locus_tag="ACG85N_000896"
     CDS             complement(14250..14849)
                     /locus_tag="ACG85N_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0223690.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            15035..15736
                     /locus_tag="ACG85N_000897"
     CDS             15035..15736
                     /locus_tag="ACG85N_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0223691.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            15746..18457
                     /locus_tag="ACG85N_000898"
     CDS             15746..18457
                     /locus_tag="ACG85N_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFQ0223692.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            18908..20536
                     /gene="treC"
                     /locus_tag="ACG85N_000899"
     CDS             18908..20536
                     /gene="treC"
                     /locus_tag="ACG85N_000899"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFQ0223693.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(20596..21777)
                     /locus_tag="ACG85N_000900"
     CDS             complement(20596..21777)
                     /locus_tag="ACG85N_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0223694.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            22029..23552
                     /locus_tag="ACG85N_000901"
     CDS             22029..23552
                     /locus_tag="ACG85N_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0223695.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(23621..24349)
                     /locus_tag="ACG85N_000902"
     CDS             complement(23621..24349)
                     /locus_tag="ACG85N_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0223696.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(24362..25642)
                     /locus_tag="ACG85N_000903"
     CDS             complement(24362..25642)
                     /locus_tag="ACG85N_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0223697.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(25783..26385)
                     /locus_tag="ACG85N_000904"
     CDS             complement(25783..26385)
                     /locus_tag="ACG85N_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0223698.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            26630..26836
                     /locus_tag="ACG85N_000905"
     CDS             26630..26836
                     /locus_tag="ACG85N_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223699.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            27148..27867
                     /locus_tag="ACG85N_000906"
     CDS             27148..27867
                     /locus_tag="ACG85N_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0223700.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(27948..28631)
                     /locus_tag="ACG85N_000907"
     CDS             complement(27948..28631)
                     /locus_tag="ACG85N_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFQ0223701.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            28849..31035
                     /locus_tag="ACG85N_000908"
     CDS             28849..31035
                     /locus_tag="ACG85N_000908"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFQ0223702.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        10401 a         5495 c         6244 g         9036 t
ORIGIN      
        1 aataaaatat tacacttgag aatagaaggt gacgtaatgc acatccagat tccaactgtt
       61 cttcttgatg gtactgtgct agcagcatta agcaaggaag atatgtatgg gtatgcctta
      121 acaaaaatcg tacaagaaag tctctctgtc agtgaatcta cactatatcc ggttttgaga
      181 agattgaaaa aagaaggata tcttgagacc tatgatgaac cttttgaagg cagaaaccgt
      241 cgatattact cgttgacacc aatgggaagt cgtcatttag aagaaattat tgatgaatgg
      301 attgtttttc gagattcttt agaccggtta ttggaggaga gcacaaatga atgaatattt
      361 gaatgaagtt gtggatcatc ttcaagaaat tcctgaagac gatcgttttg atttgatcca
      421 gtattatgag gaatattttt tagattctgg aaaaacatta gatcagatca ttgatgaata
      481 tggtacacca aagcagtttg ccttaaaatt aaaaatcagt tacttttcag aagaagaaca
      541 agcagaaaac gagttagtga aaaatccaaa aagacaaatt cgcctgattt ggttaatcat
      601 tatcggttta tttgcttctc ctgtcctcat tccactagca ctggggttta tattggttat
      661 tgggggacta ttggttgctt tactagcgct catttttagt atctatgttt tatgtatcag
      721 tatcttaggt gttggcctat tcatgatcat ctcaggaatc agtgtgtttg gacaatcgat
      781 tctgagtgga ttattcttta tcggtgtcgg tctgttcgcc acaggaagcg cagtatttat
      841 ttcgccactg ttgctgaaag gaacaaaagg gcttttttat gtcatgatga actttgttaa
      901 atggatggga cgacgtttta ttacaaaaag agatctgcat tcagcgggcg tttgaaggag
      961 gacacataaa ttgagattga aacatttttt gattaccggt attggtctga tgcttactgg
     1021 tggcctccta gcaggtgcaa cttatacatt aggcgctcaa aagagtctga cttgggataa
     1081 tggtcctcgg ttgatcgata tggttactga gacaaaaaaa atcaaggatg caaacaaaat
     1141 cgtcatccat gcagaaaatc aaacagtttc ggtcatacgc ggattggatt ttgagataaa
     1201 aacgacttat gataagacag aaaagccaga aataaaggag aaaaatggcg tgttgactgt
     1261 gaatgcagtc aaaaaatcaa caggtgcaat cattagttta gaagacacaa gtgcacaaat
     1321 cactattgtt gtccctgcag atcatgaatt atctgaattg acaatcgaag ggaataatag
     1381 tgcaattgat atcaacgaag tatcaatcaa aaaagtgaat actaagacac atgatggctg
     1441 ggtatcgttc aatgaattta caggaggaac aataactgcg aaagatgaat taggctcact
     1501 tcgattagat gaggccaatg tggataactt gatcgtcaca ggtgaaaaca gcaacttcca
     1561 tctagaagac agtttgatca gcaaagaagg aaaagtcatc ctaaacggtg gcaatttatc
     1621 tttgcatgag atggaaaaca gtggctatac tatccaaacg tctggtgaat cttatattga
     1681 aaaagactac cagccaataa caaatacttt gttagaacaa ggacagaaaa agctaaagat
     1741 cactgctaaa gacacatcga tcaatattac gacggatcaa gaggacaatg aaacagattg
     1801 atcctgtact caactggata ttttaagaaa aaacagcaaa gatccttcca atctttgctg
     1861 tttttcatcg cttaacgcac ataactatag taaaataaga aagaaaatat ctaaaaagaa
     1921 aacagggtga aagagtatat gaaggtagaa gaaggtcaaa gagaaaagtt aaaaacagaa
     1981 atggaaagat tacatactta tatcactcaa ctaagccaaa ccttctatga tccagacaaa
     2041 gaaaaagtga tggttaatta cccgaacaat tcagaaggtc gccaattaga gcaagtgtac
     2101 catgaagttt tcaaacattt attgacggtg aagaaagagc tggattatta ttctttaccg
     2161 attatcgata caggaatatt gaagtatgat gggaagaagg aacgatttat ctttaaatct
     2221 gttcgggaag atttgcctct ttcagcaggg atggatctag aggtcttggt agaagactat
     2281 tttacagaag aaaagcactg ggttcgaaca aaattggatt atttaccgca ggcagccgga
     2341 gggacacagg caagcggttg gtacatcact gaagacaaag agttagaact agaaggtgta
     2401 atggcgcgta ttcgaaaaaa aaattaaaaa agtaacatta gatatttatt gaaggataaa
     2461 ggagcgcagg aagtagcatg agagaaaaaa gagcatttta tatcaatggt tatgcaggga
     2521 ttgtcggatt ggtcgtatta gctttagttg gattgttttt cttctattta ggtatgtggc
     2581 aagcgaaagt ttgggcactt tttttaagta tttttctttg gttgatcacg ttattgttgt
     2641 taagttcggc aactgtagtc agtcctaatc aagcaaaagt aattttgttt tttggacaat
     2701 atttaggtac gattcgagaa aatggctttt tccttacgat acctttggca caaaaaatga
     2761 ctgtttcgtt gaaagttcgg aatttcaata gttctgtttt aaaagtcaat gatttggatg
     2821 gaaaccccat cgaaatctca gcagtagtag tctttaaagt cgttgataca gcaaaggcat
     2881 tattcgatgt agcgtattat caagattttg tagaaatcca aagtgagaca gccattcggc
     2941 atatcgctag ccaatacccg tatgatacat tcaatgagaa tgatttgacg cttcgaggaa
     3001 acacgacggc agtgtctgac gagttgcaaa aagagctgca agagcgttta gcagtagcag
     3061 gtgtcgaagt aatcgaaaca cgcttgaacc atttagccta tgcaacagaa atcgctagtg
     3121 ccatgctcca acgtcaacaa gcaaaagcca ttttgtctgc tcgacaaacc attgttgaag
     3181 gagctgtcac aatcactcaa atggccttag agcaaatcga agatggacaa gaaatcaatt
     3241 tcaccgatga tcggaaagtc caattgatca ataatttact tgtctcaatc attacagaca
     3301 aaggaacgca acctgttatc aatacaggcg acatgaaaga ccgtaagtga catacttttt
     3361 gtaagatagg aagaaggagt ttaggacgga ttattgtctt gaactctttt tttagttgtt
     3421 ttaaagaaaa tgacaaaatt gaaagaagaa tgatagttga ttcgaaggca gaattgttcg
     3481 cttttttcta taatcagtat ataaagtaag agagaagggg aaaaagtgaa aaaacttctg
     3541 atcggtctac ttgttttcat cgttgtggta cttgcagggg tgaaaatcgc agattacgta
     3601 gttatgggtg gagaagaata ctatgtacaa attacgacag atggtacaaa aacagtttca
     3661 aaatcagact ctggacaaga aacagtcaac tatagatata tcctgcctgg ctatacgaaa
     3721 gagggtaagg aaaaagaact ggacttcaag ggacaattga ctcgaccgct tcgtaaagga
     3781 gcatatttaa aaatcacctg gaacaaaaat aaaggtgtca cttcgtatga agaagtcgaa
     3841 aaaaaagacg tgccgaaaac agcactggaa aaactaatta aagggtaggg aaagaaatga
     3901 acaaaatagt cgaagtacag aatgtaacaa aagtatacgg aaaaaacaat gagaaaaaga
     3961 cgcaagcatt aagcggcatc agcttcgatg tagaaaaagg cgaatttatt gggatcatgg
     4021 gcgcttctgg ttctggtaag tcgacacttt tgaatatttt atccacatta gataaaccaa
     4081 cagatgggca tatccgaatc aatcaaaaag atgtgaccac attaaaagga aatcaactag
     4141 cagattttcg tgcgaatgag attggcttta ttttccaaga tttcaattta ttagaaagct
     4201 tgacagcaca agaaaatatc gctgttcctc tttctttaca aggcgtcaga ccaaaagaaa
     4261 tcaagcaacg tgtccaaaaa attgcagaac gtttatccat ctctcatatt ttggaaagct
     4321 atccttctga gatttccggt ggacaaaaac aacgggtcgc cgccgcacgt gcattgatca
     4381 cacaaccaac gattctatta ggggatgagc ctacaggtgc gctggattcc aaaagtgccc
     4441 gggatctttt agatacgatg gacgaattga atacaaaaga tcatgtatcg attcttcttg
     4501 tgacgcatga tccgttttct gcaagttatt gccaacgtat tttattcatc aaggatggcg
     4561 gtatccatca agaagtgaaa cgtggagatc aaagccgtga ttccttctat cgagaaatac
     4621 tatctattct tggaaacttg gaacagtagg gggagattga ctatgctttg gaaattatca
     4681 cttactggga tcaagagccg tttaagagac tacttggtgt tattctcagg attagttatg
     4741 gcatctgcta ttttttacat gtttgaatca atggcaagca acgaagcttt tttgaaaagt
     4801 aatacgatcg ttgcggctac agttatcatt ttccgattcg gttctgtttt actagggatc
     4861 atcacattcg tttatatttt atatgcaaat tcctttttga tgacgatgcg gcaaaaagac
     4921 tatgcaatgt ttatgatgct gggagcaaaa ggcagaaaaa tcgcacagat gattttttct
     4981 gaaacattct tagtagggat cgcagctact ttagtgggtt cagctgtcgg tgtaggattg
     5041 acaagtatcg tccaccgatt attagtggat cagttagatc tcaatatttc gcattttaca
     5101 ccttttagta ttcacgggtt gttgatcact gttttgtttt ttgctgtttt gtttttatta
     5161 gctgctgttg tcaatgcttt ttcgattgtc aaaaaaccga ttttagcatt gattcgtgcg
     5221 gatcagacac caacaagaat caagcagcat aaattcttgt ttttccttga agtagtactg
     5281 ggaattgctt ttctaggctg cggttactat atgatgaaac acgtggggac ctatcaagtc
     5341 tttggtatag gggtagcctt agttacgatc gtattaggaa cttatttcat tttccattct
     5401 gtgatcattt tctttttatc attactaaag aaatcagaac agatttcatt gaaaaaattg
     5461 aacaatttca cattgtcaca gttaaacttt cgtattcgtg aatatacaca aatgctctca
     5521 atggtcgcta tgctgtttgc cttggcactg ggcgctttga cggtaggatt aggttttaaa
     5581 aatgaaattg cagacttgac agaaaacaat tctggatatg atctagtgat gaataatcct
     5641 cgggcagaag atcagcaaaa aatcaaagaa ctttcaccaa cactttctgc ttcttatact
     5701 caaaaagaag atgcgggaac gatttactat aatgcaagtg aatttgcggc tcaaccattg
     5761 atcatgataa aacatgaaga tggaacaccg ccgaagatca cgaaagaaaa agttcctgct
     5821 aaaaaaatga atgaactgac tgtacaagag gaacttcgaa gctatgaatt gccggaacag
     5881 caagaaaaag aaatcaaggt agtcagtcaa tcagaatttg atcaactgaa cttgccacaa
     5941 tccactttac agctagttca ggtaaaagac ttccaagcaa acctgaaacc aatcgaaaca
     6001 cttgttgctc aaaataaaat gaacaatcca tctttgcaaa aagtagagta cagcaaccag
     6061 aaatatgaaa tgtatgaagt gtataatggt ctattctctg gttttgaatt tatgggattc
     6121 ttcctaggtt tagctttctt gaccatgctt gctagctgtt tgatgttcaa gatcttgtca
     6181 ggaagcaaga gtgacattgc tcgttatgaa atgcttgaaa agataggaac acgacgcggg
     6241 ttgcttaaac aatcgatccg tagagaagta ggcgtgttat tcttagcacc aggaatctta
     6301 ggtgtagtcc atgtcttatt cggattacaa atgtttggtc tattcatgca aaatccatac
     6361 aaagatattt gggttccgtt tacgatattc ttcgtattgt atttcatcta ctatgtactg
     6421 acgacatggt tatatactgg aatcgtattg aaaaagagat tgtgaaaaag ctgtcctgca
     6481 tcaagtaata agaacagcca agcaaaaata gctcctcata tttttcgttt ggttgggctt
     6541 attaccgcag atgcaagctt ttgaacaccg tttgttcgga aaaagaggtt gtgacaatat
     6601 ttttgtcaca accttttttt cacggtcttt tattagaata tagtaaattt tttctcgtta
     6661 ctcacggaat aagctgttaa gaaaacggtt tccatgtata ctggaatcag tgcaaatgaa
     6721 agaaaagagg gtgcaaggaa catggaaatc aatgaacaaa attttggcga aaactatcac
     6781 ttgattacga tcacgaatca aaatgggaca tctttgtctg tcagtgatct tggggcacgg
     6841 gttgttcatt ttcaaacaat aatcgataat gaaaaacgag agttgatctt aggttttgat
     6901 gaagcaaaag aatatctaga aaaagaccct tatatcggtg cttcaatcgg acgtatggct
     6961 ggacggatta cgaatggcac attcaaatta gatgatgaaa cgttcgaagt aaaaacagac
     7021 ccggacactg gtcattgttt acatggtggt gcacctagct ttgaagccaa gcagtggcat
     7081 tatacagtca tcaatggtga aaacgaagca tcggtcattt tctatcagac tagcccagat
     7141 atggaaaacg gatttcctgg aactttagat gtagaagtac ggtatacgct gacgaacgat
     7201 gatacatggc gagtgacggt ccaagggatc agtgaccgca aaacagtatt caatcctaca
     7261 aatcatgtct atttcaattt aaccggtgat ccagcacagt caatcgacga acatatactt
     7321 tggttgaata gccgattatt tgctacgctc aataaagata caacgcctac aggagaagta
     7381 gtttcagtaa gtggcacagc atttgacttc caacgtccta aaaaattagg tgaggtcttt
     7441 gccagtgatt ttcgccaatc aatgatagtg ggcggcatcg atcatccatt tttgttgaaa
     7501 aaacaatctg gtttactaaa tgctgcggaa ttgacaagcc cagatgaaaa agtatcgatc
     7561 caagtaaaaa cagatgcttc aggaatcgtg atttttacag caaattttgg agaagatgga
     7621 ccggaaatgc gcgggaataa attagtaaat catggcggta tcacatttga gactcaagaa
     7681 ctgccaggag cggagcgact tgaaagtttt ggagatgtca ctctggaacc taaccaagta
     7741 cgcgagtttg taacggaata caaaataaat ataaagaaat agaataaatt aaaaagggag
     7801 tgtgacagtg tcacatcccc tttttaattt attctatttc tttaaaggca gatcaagcca
     7861 gaatgatttg tctaaactta acaaggttag tacaggaaca gttgcagctt taactgtacg
     7921 agtagttgtt tctggtatct gtcttgccgt aaaattgttc cacaaatccc agattgaagc
     7981 gacacggaag atggaagaag cgttgttgac gcgttttatc gttgaaaatt ctaatcctgt
     8041 gattggcacg ccatctgttg tccatttgat tggcacatcg aatcgcttag acatattgag
     8101 gatttcagcg tttgcttctt cagttgttgc tgcttctttt ttcggttcat catattttgt
     8161 caggtcgtac gcttcgatga tcgcgttcaa tttttgtgaa tattggcgat ctgtagcata
     8221 acgtccttcc aaatatttgg ttgcttcttt gtagtctttt gtttccgact tccaagttgg
     8281 tttgtagaaa tcaggattgc cagtcagtcc atttttgatc aagtcggcat agtcttccaa
     8341 actttccttg taagaaggat atttacggaa ttgtgcgcgg atttggtagc tttgtccgga
     8401 actatcttgt tcaagtgtat tgaaggtcac gctgcttcct ttatatgatc ctttgatacc
     8461 aaaaaggtta tgatttggtt cgcttgataa taagctgttt ccagatccac tttcaaggat
     8521 agcttgagcc atcatgactg atgcgtagat tccttctttt tgcccgattt cctgtgcatc
     8581 atctgcaatc tttttcaaga actgatcagt tgtttggttt tgactgaaat ggaacgagcc
     8641 atctaatcca tctaaaatag atgagccgcc tgtcagcgaa cctgaagcag caggtaaacc
     8701 tgctaagttt actcggacag caaaatcagg cgtgaaatct gaaatagaag ttactcgaac
     8761 tttatctcct ggttgaggcg catggataaa ttgtccgttc ccaatataga tacctacatg
     8821 gtgagtaaca ccggaagaag gtccccagaa gacgaggtcg ccaggttggg cctgacttac
     8881 tggaatctgc gtacctgcat attgctgttc gcctgtccag ccaccgatat aatgaccggt
     8941 tgctttcata tatacgtaat aagtcaaacc agaacagtca aaagtatttg gaccttttgc
     9001 tccccataca tatggttttc caagatgctt gaatgcttct cgaacgattg cttgttggac
     9061 attcgttgta tttgtaggca atacagcatc aattggtgat tttacaacaa aatcagaaga
     9121 agcagaagat ggattctcta taccgctttg atttgctgta tttggacgag atgaagcatt
     9181 gttcgttccg ccattcgatg aaccattgtt tggatggttt ggttctctct gagtatttcc
     9241 attgttattc gatccagatg ggtttgtttg attcgaagaa ttcccgctgt tcgaattatt
     9301 gttattattg tttgaattat ttgaaccatt attattgttg ttattaccca catctggttt
     9361 gtttggcgtt tccggatctg gtgtactagg gacttcttca ctaggatcag ttggtttctc
     9421 tgttgaagta tccggtccag ctttaaattc tgcagtaata gataaattgt tcgtacctac
     9481 tgtaaaggtc tttgcatcat aatttacttc tttggcacca ttaacagcga ctgaagcaat
     9541 ttgttcacct tcagctggcg taatagtgta agttaccttc gtttccgcta ataaaccttc
     9601 taccgttcca ctttccgtaa tatccaaagg ttgctgttca ggggaatccg ctgtcgaagc
     9661 agtcagttgg atcttaccaa tcaaagaagg agcaattgta agggaatatg tctcggctgg
     9721 cttttcattt tctgatggat cagccggatc aatttcagtt ggctcggtcg atcctgttgt
     9781 tgagttagtt gttccagttg ggtcttctgt aaaagaatcc gttgtttcac tagtagctga
     9841 gctggatgta gtcgtatctg ttgtgactgt ttgatcagct atttgattat ctacggcaaa
     9901 tacggtattg gtcgacaaaa cagacggagc aatcagccaa gtagcagcta gcaaacgtat
     9961 cgttttttta tttttcataa taaactagtc cttttccttt cattgatttt ctgtcttatt
    10021 cagcattcat tagacatttt ttggaacaga taatcgtgtc gaaaagcttt ttcttttcat
    10081 aaatctgtct gtgtattcta aataaaacaa tttgtaaata agcaacgagc attcattcgt
    10141 ttttctttga cgttcatttt attgcataga ccacctttgt ttggggataa acaagtgggg
    10201 ataaaaatgg ttatgcatat gtaaattata ccaaagtttg gaaagtatca caaaaattta
    10261 taaaaataat gatcctaaaa agaaaaagac tgaaatatgt gataaacatc tcatacttca
    10321 gtctttttca ttaactattc ataagacatt aggtcttcat caaacccgct aacgagagta
    10381 gtgatcattg ctacgactag actaacagcc agtcctgcta agtaccagaa aaaagtaccg
    10441 gaaatgaatg caggcaatgt ggtcagacta ccaaatacaa aagcgatcgc tttcacttgg
    10501 gcaaatccca ttacagctcc accagcagct gcaccaatgc aagcagcaac aaatggtttt
    10561 ttcaatggga tcgcaattcc gtaaacagtt ggttcggtca ctcccgcaag taaaatcgaa
    10621 gcaaaagcag aaccggagat cgaacgggtt tttgaacgtt tacttcgcag aaatacacct
    10681 aaagctgcac cagccattcc aaaattggct gctcctgtca acggaacgat tcggtcgaac
    10741 ccataagcag agatattact gatcatgaca gggtttacag cccattggat accaaatatt
    10801 accaaaatat tccaaccacc agcgatgatt gcgccactga tcggtccgtt aatatccatc
    10861 atccaagtaa ttgcggcagc taaaccttcg ccaaggtaaa cacccgcagg cccgaatacc
    10921 attaacgtca aaggaaccgt gataactaat gaaagtaaag gtacaaatac taattgcata
    10981 ttttcgcgaa tatatttttt gaggaatttt tcaacaaaag aataaaagaa aatacctaaa
    11041 atcgccggca tgacagtaga cgtatactgc atcagtacta caggaatccc cagaaaatca
    11101 gtagctgctt tgccactgtc tgaaagtaaa gaggtcagat tcggttcaat cagtgctgca
    11161 gcaatgacag ccgcaatata tccattcact ttaaacgttt tggcagcaga aaaagcaaga
    11221 aagattggca gaaaataaaa gacggcattc gacgcagcag acaaaatatg ataagttccg
    11281 cttgtttcac taagccagcc aatcgtagtc gctaaaacaa ccagaccttt taatatacca
    11341 gaaccggcaa acagcggaat gatcggcgta aagatttccg atatcaaacc aaatagtttt
    11401 tcgatcggat ttagcttttc tttcggagct gaatgatcga tttgacgcgt caattccttg
    11461 taagcaggag ccaatccctg atccatgatt atttctgcat atctatcgag taaaatgaaa
    11521 tacttaatgg aagggatttg ctgttttaat ttttctgcat tggctactgg ctgttttttc
    11581 aatttgatac gaagccgagt cgcacatgga aggacagaat caatattgtc ttttcctccc
    11641 aactgttcaa tcagcatatg aatctgctcg gacagtacca tctgttcagg attcattgtc
    11701 gtttccatta gaagaccact ccactcacta atataatatt ggcgtaaggt gtcatttcgc
    11761 ctgtacgaat aaatgcatga cagttattca actctttttt catttcttca tgtgggataa
    11821 agctaatcgg tgtatctggt aacaataatt tgattttttc caatatttcc gggttcattg
    11881 tctttatttc ttccgccaat tcaatcgatt gtacctcaag ttcttctaaa acgttttcta
    11941 aaacagataa aaaacttgga agaccgtttg agacagccaa atctattttt tcagtaccaa
    12001 aggggacagg catgcctgca tccccgatac ttaatttatc aaagtgcccc atttgtgcga
    12061 ttactcgtga aatatctgaa ttgattactt ttcctttttt catgtttgtt tcttcctctc
    12121 attgttaatt ttagactgct gattgttcta aagcttcgcg gtaagggata gaaggttgcg
    12181 caccataaga ctgcacagca acagaagagg ctagatttcc ataaaggatc gcttctttca
    12241 agttactaag gtctggctga agtgtgctgc ttaatgcgcc aataaatgta tcacctgcgg
    12301 ctgttgtgtc aacggcatcc actttaaaag cagggacgat accgtgttct ttttctgtat
    12361 gatagaaagc gccggcactg cctagtgtaa tgattactgt tccaatacct aattcatgga
    12421 gtttttcggc tgctgcgacc aaactgttat gatctgtcac tctgattcct gtaatgattt
    12481 ctgtttctgt ttcatttggt ataatgatat ctgtcttttc aagtagttca gtaggaatcg
    12541 tttcacgagc tggtgcagga ttcaggatcg ttgtttttcc tgcgtctttt gcaatagaaa
    12601 aagctaggat cgtcgcatct aagctacttt caaactgagc aataataaag tcactgtttg
    12661 taatcagttg cttgtttttt aggacttctt gttcatggaa agcattattt gcccctgcat
    12721 ggatcaagat actattttct cctgctgcgt ctaccataat cattgcttgt ccggttgtgg
    12781 cgccttggat ttcagctatc ccagatgtat cgatgttttc ttttgtcagt agatctaata
    12841 actgttgtcc ttcgctgtca gccccaacgc ctccaataaa agatgtccgt gcgcctgaac
    12901 gtttggctgc cactgcttgg ttggcacctt tacctccgcc agcgtgaaaa atctcatgcg
    12961 catgcatcgt ttcgcctggc ttgggcatgt tggttaaacg aagggtagtg tccatattaa
    13021 tacttccaat tactgtaacc gtgttcattt tacaaacctc caactatctc atttattttg
    13081 atgggaaaat tattttttaa aacgttttag taaaacgttt taatagttcg ataaaaatat
    13141 agcacacctt gaaagctatt tcaagggtgc agttgaaaat cttttttcta atcgtacagg
    13201 taatcttttt tcttcccatt ctttttctgg gaattggatc cgatctaaca ataacttggc
    13261 agatgtttgt cccaattcga agattggttg tgcgatcgtt gtcagtttcg gcttgatata
    13321 ctcgcacata tctatattat catagccaat aatgctgtag tcttcaggga tgctttttcc
    13381 agcttcctct aatccgcgat ataatccaaa agctaattca tcatttaaag caaacacacc
    13441 agttgcagca gaatcaagaa gttctgctgt gatctggtag ccaccttgtt ttgagaactg
    13501 tgtaggtaag aggatcagtt ggtcgtgagg gatttggtaa acatcaagag cagatttaaa
    13561 cccctcaatc cgagcgtgga cattttccgg tggattttca ggatagacaa gggcgatcgt
    13621 ttgatgtcct agactaagca agtgcatgcc cgctaggtaa ccaccgcgaa aatcgtctgt
    13681 tcgtacggca tcactaaatc cttcagattt tttctgatcc aaaacaatga agggccggcc
    13741 ttgttttttc aaattttcgt tgattgcatc tgtagagaca gcagaagtcg cgataatgaa
    13801 tccatccaca cctcgacgtg tgagctcggc tagatattcg atttcttttt gatgatcaga
    13861 atcagcatta cataatatcg tgacgaaatt ctgcttatac aagatatcct caattcctcg
    13921 catcagtgta ctgaagaatg gattcgtgat atcaggaacg agtacaccaa ttgttttcgt
    13981 ttccttggta atcatttgtc tagcaaaata gtcaggctga tagcctaatt cttctttggc
    14041 agctaaaact ttctgaacag ttttcggact gaatttctcc gtatttccat ttaatctcaa
    14101 ggagacagtt gtgattgaaa cgccggaaag tttagcgact tctttgattg tgatcttttt
    14161 tttcaccatg ttcatactcc tatctaaatc ctgtggttat tttcccatga aatcaaaaag
    14221 aagtaaatga aaatcacgtg tgccagtact tatttgcttt gttggatagc ttcgattatt
    14281 tgtttcgttg agcgtactcg tgcagttttc ggaaatatct tttttatgaa caattgatgg
    14341 gattctttgt cacgatcact gcaagcatct tcagcaagca cgatatggta cccgtgttga
    14401 aaggcatcta atgcagtagc atatacaccg tttgttgtcg ctacaccgct taaaatgata
    14461 gtttcgattc cacgccggcg aagttgaaga tcaagagaag taccaaaaaa agcacttgga
    14521 ttgtattttg tgatagtcaa catattatct gtatctttca aagaatcctt caaaagtaaa
    14581 tcagtgaatt ctggaggtac agaagcagtc attccaccca tatcagtttg ttgactcaaa
    14641 taagggaaag tggagatgtc tacgttcact aaaactttca aggcagcggt acgtttcaat
    14701 gttcttacta acaaatcatt gtttgtcaga atagatgctg cagtgtgtgg agagagtgta
    14761 ccgttattta cgatctcttt ttgcatgtcg atcgcgacaa aagcagtttt tgataaatca
    14821 aaaatcatat tttcctttaa atcattcatg atgttccttc tttcaaataa caatgttttt
    14881 tatgtaagta aatcataaat cgtttatttg tcaactaaat taaaggattc ctttctttag
    14941 tctagagaaa ttcagtggta aaataaataa gtgtgagaaa acgcattcga tgcatagata
    15001 tagcgagaaa tatcgtgaaa atggaggaat cagagtgaag tatgtcgaag taaaaaatga
    15061 atacaaacga tacaaaatgg gagaaacgac cattacagca aatgacgata tttctttttc
    15121 aattgaaaaa ggcgaattag tgattatact tggtccaagt ggagcaggaa agtcaacgat
    15181 tcttaatatt ttaggtggga tggatacacc tgatgaaggt cagatcatta ttgatgacac
    15241 agatattgcg caattttcag ataaacagct tactgcttac cgacgaacag atgtagggtt
    15301 cgttttccaa ttctacaatc tcgttcctaa tctaacagca aaagaaaatg tggaattggc
    15361 aacggaagtt tctcctgatg cgcttgatcc tgtagaggta ttgaggcaag tgggcctagc
    15421 tcatcgattg aataactttc cgtcccaatt atcaggaggc gaacagcaga gggtttcaat
    15481 tgctcgtgca ctagctaaaa atcccaaact tcttttatgt gatgaaccga caggcgcgct
    15541 ggattttgaa acgggcaagc aagtattgaa attattgcaa aacgctagca gacaacacgg
    15601 caataccgtt ttgatcatta cacataattc tgctttagcg cccatcgctg atagagtcat
    15661 tcacattaat gatgctaaag taaggtctgt tgaattaaat gatcatcctt catccattga
    15721 tgagattgta tggtaggggg attttatgaa aacaagaaca tatcttaaag caggttttcg
    15781 cgaaattcgg caatctaaag gacgattcat tgcaatcatc cttattgtca tgctaggaac
    15841 acttttattt gtaggtgtca aaactgctgg acctgtcatg caaaaaacga tggatcacta
    15901 tgttggtaca gcaggacttt ctgatttgca aatcgtttct actggtggct tgacacaaaa
    15961 agatattgct caagcagaaa agatcccaga tgcaaaagta gaaacaggaa aacagatcta
    16021 ttattccaat actggcaaaa acgaagtgat tcaaatattt tcttataaca aaaataacaa
    16081 gcagaacaag ctacaattaa ctgatggaaa actcccggaa aaaccaaacc agcttgtatt
    16141 ggatgaaaaa gcggaagatg aagggtataa aatcggtgat atttatcaga ttgattcaga
    16201 tgaattgaaa gaaaaagagt ataccatcac gggctttgtc cgttctccat tgttcatcaa
    16261 caatttggag agaggatacg cgaacgtcgg aaatggtagc gtcgattatt ttgtctattt
    16321 acctgaatcc gtttttaaaa cagatattta ttctgtctta tatcttgatt ttacaaacgt
    16381 aaagaatttg aatacgtaca gcaaagctta caaagataaa atggagcaaa atcaagaaaa
    16441 ggcagaaaag tatttacaag atcgacccga agaacgctta gaagagctga aaaaatcggc
    16501 caacgaagaa ttagaaccgg cacagcaaaa aatagcggat ggcaaagcac aaacaacgca
    16561 agcaagaaca cggttagaag atgctaaaaa acagttagcg caacagtcag caactatcca
    16621 gcagcttcct gaagcgcaac gaacaatcgc tgttcaaaca ttatctcaac aagaagctca
    16681 acttgctgaa caagaaaggc aattagttga gaaagaacgt gaactggcct ctgcagagga
    16741 agaattgaat cgaaatgcag aaaaaattga aaatttaact aaaccatcat acctatacaa
    16801 tgaacgttca gaaaatgtcg gttttcaaga atttggtgat ctgtctgatc gtatcgctgc
    16861 gattgccaat gtttttcctg ttttcttttt ctttattgcc gcattgatca cctttacgac
    16921 aatgacacgt atggtagaag aaaaccgcag ggaaatcggg acattaaagg cgttaggtta
    16981 tacgaaagtt gaaatcgcaa aaaaatatgc aatctatgca agtctagcct cagggatagg
    17041 tattgtttta ggaacgatat taggaacgaa tcttttgcca aggatcattt ttgagctgtc
    17101 taacgagcgg tatgatattg ggagtgcgct tattttttat gactggccgc cgattttaca
    17161 agcagctgcc gcattcttca ttgccgcatt tggcgctgca atgatcgttt tattcaaaga
    17221 tttaagagaa cggcctgctg cattgcttca accgaaagca ccgaagccag ggaaacgcat
    17281 ttttcttgaa tacatcactc cgctgtggtc acgccttagc tttaatcaaa aaatcagtta
    17341 ccgaaatctc ttccgatata agtcaagaat gcttatggcg attatcggaa ttgctggatg
    17401 tgccgggctg atggttgcag gcgtgggatt gaaagattct ctaagttctg tttcagccaa
    17461 acaatttgga ccgatcattg actatcaagc aatcgttaca ttggattcat ccacggaaga
    17521 tagtcgtcaa aaagcagcgg atatgctgca gaagcaagaa aaagtaaatg agatcctagc
    17581 tattcatact caaaccgtag aattgagaaa aaaaggacat gcttcgcaaa gtctgacagt
    17641 gatcgtacca agtaaaacag ctgatttgga tcgttttatc catttaactg atcttgaaga
    17701 gaacccacgc tccttgccta aaacaggaat cgctattacg caaaaagtag cagaactatt
    17761 tgacttatct acaggagatc atatttcgct ttatgatgaa gatcaaaaag aatggaaagt
    17821 cgaagtaaaa gaaatcgtga aaaactatct aggtagcttt atttatatgt cccctgaata
    17881 ttatgcttca gttactgatc aatcgatgat ggataatgca tttttgctgc gtacagataa
    17941 gatgacaaca aaagaagaag aaatgctttc cgagaaactg cttgccacag acgctgtgac
    18001 aaatacgtct ttcatgtcca aacaaatcga aacgcaagaa gaatcaatgg cgaacttaga
    18061 ctcggttgtc ttaatctttg tcgtattatc aggactgctg gcttttatcg tcttgtataa
    18121 tctaactaat atcaatattt ccgagcgtgt acgcgaatta tctacaataa aagtccttgg
    18181 attttttgat aaagaagtca cgatgtacat tgttcgagaa aatatcatct ttactctttt
    18241 aggaatcatc ggaggatttg gtgtaggata tatattgact gacttcatct tgcaacaagc
    18301 gtctatggag aatgtcatct ttccattagt gatcacatgg tgggcttatg tcttatcagc
    18361 aggattaact atcgtattta cggttattgt gatgattgtt acccatttca aattaaagca
    18421 tatcgatatg attgatgcgt tgaaatcgaa tgaataaaaa cagaggttgt gaaatcagcg
    18481 gtttgacctg agtattagag aaacaaacgg caaaatagtt tttcatattt tgccgttttt
    18541 tgagctaata ccgaaggaca gctgattgaa caccatgaat cagaggttgt aaaaaagccg
    18601 tcctgcatcg agcaaaaaga atactcaaat aaaattagct cctcatgttt tacatttgag
    18661 aagactgatt gccgtagata aaagcttttg aacaccattt atagaagaaa caagaatatt
    18721 ttgctatttt aaacaagagg atagacaaat acatgtctga tctcttgttt tttattgggc
    18781 aacagacctc ttagaatgta taaagataaa ttttacaaac gctttcggtt cagttataat
    18841 aataaatgat aacttgtata tataagttgt ggtaggtaat aaatcagttg aatggaggta
    18901 aatatccatg acatttaaag acaaagtcat ttaccaaatc tatcccaaat cttacaaaga
    18961 cagtaatggt gatggaattg gagatttaaa aggaatcaaa gaaaaactcc cgtatctgaa
    19021 tgatctaggc atcgacatga tttggctaaa ccctatttat ccaagccctc aaaaagacaa
    19081 cggttatgat atctctgatt acacagccat cgatcctatc tttggtacga tggaggaatt
    19141 cgaacagcta atagccgaag caaagaaata ccggatagaa atcatgctcg atatggtgct
    19201 gaatcacgtt tcgacagaac atgaatggtt caaaaaagca ctagcagggg accagcatta
    19261 tcaggacttc ttcattttaa gagaagagcc aacggattgg gtatcaaaat ttggtggaag
    19321 tgcttggtcg ccatttggag atacggggaa atattatttg cacttatatg atcggacaca
    19381 agctgacttg aactggcgga accaagcagt aagagaggaa ctattcaaag ttgttcgttt
    19441 ttggatggaa aaaggggtaa aaggattccg tttcgatgtt atcaatgtta tcggtaaaga
    19501 cgaacaattg aaaaataata aagaaaatga tggcaaacct gaatatacgg acaaaccaat
    19561 cacccatacg tatctaaaag aattgaacca agcaacgttt gggacggatt ccactatcat
    19621 cacagtcggt gaaatgagct ctacgacgat cgaaaattgt atcttgtata cagaacctag
    19681 tagagaagaa ttatcaatgg tctttaattt ccatcatttg aaagtcgatt atgaaaatgg
    19741 gaataaatgg tctactcctc catttgattt tgcagaatta aagcgactat ttcatacatg
    19801 gggagaaaag atgagtgagg gtaacggctg gaatgctcta ttttttaata atcatgatca
    19861 gccacgtgcg ctcaatcgat ttgtagctat cgatcagtat cgaaaacaag gagcagagat
    19921 gctagcaacc atgatccatt tgaaccgtgg gacgccctat atttatatgg gagaagaaat
    19981 cggcatgatc gacccagact attcatcgat ttccgactat attgacgtag aaagtttgaa
    20041 tgcttataag ttactccttg aacaaggatt cacagaaaaa gaagcgtttc gtcggatcaa
    20101 agcaaaatct cgcgataact ctcgaacacc catgcaatgg tcagacacag aacaagcagg
    20161 atttactaca gggaaacctt ggttaaaagt tggaggcaag ctagaagaga ttaatgtaga
    20221 aaaagaacta tcttctgaag attctatttt gtcctattac aagaaattga ttcggctgag
    20281 aaaaacatat ccaatcgttg cacaaggaga ttatcatgct tatgaagcaa atcatccgga
    20341 agtttatggc tttcttcgcc aatttgaagg acagcagctg cttgtattaa caaatttcta
    20401 tgataaagat acaaggatcg ccatcccaga aggattcaca gaagccaata ttcttatcga
    20461 taattattcc gatactgttc ttcatcaaag tctggagcta aaaccatacc aggcaattgc
    20521 tttgctgaaa aattaaaaca aaaacacaaa acgtcgttcc tacgagctat tctcgtaaga
    20581 tcgacgtttt gtgttttatt taaaataatc ttcttcagaa aatgaagagt tttcatactc
    20641 tcctttatcg ccataaaatt tattgtagcg ttgtttgaat ttacggaaaa taaaagtaac
    20701 gagtaatttg actaacgtat agataggaat accaaaaata acgcccacaa ttcccattag
    20761 atcacccatg acaagtaaaa cgattaagat cgtaatcgga tggatctgta aacgatcacc
    20821 cattactcga ggaatcacca agtcaccgtg gagaagttgt acggcaaacc agacaatgac
    20881 aaatttgaca gccataaaag tagagtcttg gaatgcaatg atcaaacctg gaaagaaggc
    20941 gataaacggc ccaatatatg gaatgataca taacaaccca gcagaaatag ctaaaacact
    21001 agcaaattct aatccgatca gtaagaaaca aacccagtag actgctccaa ggataaatga
    21061 agcaatgatt tgccccttta gaaatgaacc caattgttga ttagcaatct ttgtcaaatt
    21121 tttaaaatct gtacgaaatg ctggtgggac aatcgctaaa actgactgat aaaatttttg
    21181 aggatctctc aaaaggaaaa aagcgacaat tggaccagta aataaagtaa taaaaatagc
    21241 tgtaacagtt gaaaaaatgt tgctgatccg ttgtgcgcca ctttcccaat aatttccaat
    21301 aaatccagct atatcagacg tcacgtcgtc aatagaagcg aaaaattgat taaatgaatc
    21361 ggcaaaaggt gttttctcca aaaattgtcg gagactctct tgaaaatctt gaaacaaatc
    21421 tggcaaacta ctgatcaatg tctttgcctg ttcttcaaga aaaggaataa gccatatgct
    21481 tccaagcccc aataaaaata aaataatcac ataaacgata atgactgaca agttcctcga
    21541 aaggtgcttg ctcaaacgtt tgatgactgg ttccaaaaga taatacaaga tcagtgcaaa
    21601 caataatggg ggcaagatag tagcgataat gattccgaca ggcttgaata taaacgagat
    21661 ctgatggaaa aaataaatga tcagcgcaag taagataact attcctaaaa catagtaaga
    21721 ggtttttcct cctaaaaagc ggatcaaacg attcgttgta tctgtttctt ttttcactct
    21781 ttttcctcct ttatctataa attcattata gacaaaagcg ggtataaaag gaaaatgata
    21841 gaggttgatt gcaaaaatat cgtcaatcat tcatataaac tttctcatca attaaaataa
    21901 gaaaacgctt gacagttctt ttaaccatat gataaattaa atcctgttgc taaaacgtaa
    21961 ctattacggt ttgcgaaaag cgaatcggaa cattcaaatg aaagaagatg agaagaggag
    22021 tgtcataaat gaaacgattg ataggtatat tggccatgct ggttttagca ggtatgttaa
    22081 ctgcttgcgg ggcaagtggg aaagccgagg acagtaaaga aaaattgagc gtgatgacga
    22141 ccttttatcc catgtatgat tttacaaaag caatcgtcgg ggatgaagga gaagtagagt
    22201 tattgattcc agcagggact gattcccacg attatgaacc ttctgctaaa gacatggcaa
    22261 agatccaaga tacagatatc tttgtttata atgatgaaaa catggaaaca tgggtccccg
    22321 caatccaaaa aacattacaa gaaggcaatg tccatacgat aaaagcgaca gaaggtatgc
    22381 ttttacttcc gggaagtgaa gaaggacatg atcatgacca tgaacacggg gaagaaggcc
    22441 atacacatga gctagaccca catgtatggc tagctccaag tttagcaatc aagcaggtag
    22501 caaacatacg tgatcagctg attgaagctt acccagaaaa acaggaagta tggacaaaaa
    22561 atgcagcagc ctataccgag aaacttcaag cactccatca gctttatcaa gaaaccttta
    22621 agcaagcaaa acaaagatct tttgtcacgc aacatgctgc attcaactat ctagcgttag
    22681 aatatggatt gaatcaagta tcgattgctg gtctttcttc tagtgaagaa ccgtctgcgg
    22741 cgagaattgc tgaactgaaa cactttgtta aagaacatgg aatcaactat atttattttg
    22801 aagaaaacgc caaagatagt attgccagaa cgttagcaaa cgaagcaggt gtatctttag
    22861 aagtattgaa tcctttggaa ggattgacga atgaacaaat agaaaatgga gaaaactatc
    22921 tttctatcat ggaagcaaac ctcgaagcgt tgaaaaaaac gacagagaca gaaaacccgc
    22981 tagaagatac tttgaccccc aaaaaagaaa agagcgtcta caacggttat tttgaagacg
    23041 aggatgtaca agatcgctct ctttcagatt gggcaggaac ttggcaaaca gtagatacgt
    23101 atgtagaaga tgggactttt gatcctgttt ttgaatataa agaaaaattg aatcaggata
    23161 agacagcaca agaatataaa gaatattata cgaagggcta tcaaacagat attgcttcaa
    23221 tcaagatcag tgagaaagag atggcattta cgtttaagaa tggaacaacg aaacgttcaa
    23281 attatcgtta tgtagggaaa gaaatcttga cctacgaggc aggaaaccgt ggcgtaaggt
    23341 atctattcga agcagaagat aaggaaagtg gcgcttttag atatattcaa ttcagtgatc
    23401 acacgattgc accggctgac tccgaacatt ttcatttata tgcaggaaat gaaagtcaag
    23461 aagtattgct gaaagaaatg gagaattggc caacatttta tccagataca atggatggac
    23521 gtgaaattgc acaggaaatg cttttgcact gataggcaaa aaagaaccga agggtttgga
    23581 tcggctggtc atttagtcgg tccaaactat tcggttcttt ttatgaaatc aatttcttta
    23641 atccacggat agaggctggg atggtctcgc cgtttgccaa cgtgacttct ctggtttttt
    23701 tattaatcga ccgcacattt tgtgtattga taacatagga tttatgacaa cggataaaat
    23761 tccgatgcat tttctcgatt tctttgatct tctcatagaa ttgtaattgg gcatgagtca
    23821 aatggacttc cagtttatgt ggcgctccta gcgaagtaga aaaatattgg atgtctttga
    23881 tcggtaaaaa tttgatctca tcattaaact ctattttgaa gatatctttt tggatttggg
    23941 acatttgaaa atcttcgaaa attttgctca ggatatgctc gacattttct tttaaatcat
    24001 aaagatcttc tttgacgata taatcagtcg gttcgatgtt gctttttaag attttcatcg
    24061 acatttccgt atgggaagtc acatagatta tttttcctaa aggatctaat tcacggatca
    24121 atttaccgat cgctacacca tccatttcac tgtcttctag ttcgatatct aaaaagtaaa
    24181 taccaaaaga agaagttctg atcttggctg cattgagaag ttcttccgga tcagaggtag
    24241 aaatatggac ataactgtct aagttttcaa tcatgattct atttttgatc accgttgata
    24301 aaacttctaa ttgttttgga tcgtcttcac aaatataaat gggaatcata tcattactcc
    24361 tttattttat ttgtatataa atcgtttgag aaaaacgctg gttttcaatt gatgtttcaa
    24421 ggaaaagata aggattggtc tttgtcaatt catccaagat aaacaagcca aggccttcgt
    24481 tttttggatt tgtttttgtt gagaaccctt tttgtttcaa ctggtttaac ggtaatgtat
    24541 ttgttgttgt attgatcaca cgaatcatca cggaatgatt cttcttgagt atggtcaatt
    24601 cgattttttt cttctttgca gcactagctc cttcaatcgc attgtccaag ataataccaa
    24661 gcatacgtac aaggtttacc gtatgtttcg tatccagttc gattggctca ggaaaatgaa
    24721 gctggacgat caaccctttt tcctgtgcca tcatgagttt taatgacagt aaactacgga
    24781 tctcaggaac tttcatatta tccaacatac ttagattggc cggaaaaagg ttcatcatct
    24841 ggcgtgtagg taatatcgtt tgatcataat aagaccgtaa tccttctaag tcgccttctg
    24901 aaatatagct gtgaagagaa aaaagcagat tttggtaatc atgtttgaat tctctcaagt
    24961 cttgatagtt tttttctaga tctttgatat aatcctgttg catcctgact tgttgctcta
    25021 ataactgatc agctaatttc ttattagaga ttttccaata ataaaccaag atgatcaggc
    25081 caacacacag atatagaatg aaaaaactcg tattcaaagc taagacatca ggtgtttcac
    25141 cagaaaaacg ggtataaaaa ataatgatat agtatgtaaa taatatcaat gtcccgatcg
    25201 taccgatcat acggcggtcc acagtgtgaa accaagtctc taacgatttt tttaaacaaa
    25261 aagctaataa agcagataaa aaagcggata agacaatgtg aaaatactgc aatgaatctc
    25321 ctggttcaat aaccgaagat ttcaaaacaa aaaaatcgcc gatgcttgtt aagtgatctc
    25381 ctaaaagcga cgtcaataac ccaaaaggaa gcgcgtaaac caattgttga cgcgaagtcg
    25441 aaaagaagaa aaaagtaagc atcagcattc ctatagcaat tagagcagta aaaatcccta
    25501 aaatcgaata gacccatcca atcaaaatac aagaaatcag aatgactccc gcaaacagat
    25561 atttgtatcg gatgtgatgg tcaatcaata agatacaaat tgtgtaaatc aaatattgca
    25621 acgctatgga ccataaaaac ataaagtaca gctcccattg atttttacca aattatagca
    25681 caattggcgt attatttttt taatttttca gggattttga attcagaact cataccaaag
    25741 cagcagccag atacaaaaaa gtcagataag ttagttaatt gtttaatgat tgtttttttc
    25801 atgaaagatt cctccaaaat tcatcaagta gtttgtaata ggaagcaacg tagctatttc
    25861 taaaacaatc ccaagtagca aataggtaga aaagtaagaa gctgagaaaa ataacgtaat
    25921 gagagaaaaa attagaaaca tgctgatcgc cttctttaat aatgggggac gtttctcggg
    25981 ttcaatggga ttgattgctg tacctcttga tccatagcga agcagtaaca aaaagcagct
    26041 gattaacaaa aagaccatca ttgggaagga gatagtcaat tgcagatgtt tcatcaccaa
    26101 gggaacgccc cataaataaa gtagtgtcca cagcagacaa acgaattcag agtcggaatg
    26161 caatccataa gcatatctgc ggatgcagat ataagaaaga aaggtgattc ctgtttccgc
    26221 ccatgagcct gtcaccagag aaaagactag aacgaccgct agttttccaa gattattcaa
    26281 taaaacttct aagacaaatt ttacttggac atacaccata tcttcttccg atttgtctcc
    26341 gctgaataag gcttctaata atttgttcac aactttatct ttcatctcat acgtacctcc
    26401 accacaaatt ttatgctaaa aaaaataaaa aacgtctttt gtttcataaa aagtggtttt
    26461 gacatcaaaa ccgtcatgat ctcctaaaaa aatcttcgtt ttccaagaac cttaccaaaa
    26521 aaagtgacag ttttgatacg aaaagtactt tttatgacaa gtttgtgctt tttcgagtga
    26581 tttatgttaa aaaattactg accttataaa aggtgatgga gggattctta tggaaaatga
    26641 acaattgttt gaagtcggcg aaacatatgc atttaaatct gatttatttg aatcaccaat
    26701 aaaaggaaaa atagaaaaaa tctatgaaaa tagtgtgatg atacgtgtca tgaaatgcgc
    26761 agtagaagac aatgataccg ctcataatct gcactggcag ttagttgtac gcaaatcaag
    26821 tacaatagaa gaataaaaac agataaaatg ttgagagcat aaagaggttg tgagtctccc
    26881 gtttagctct gagttacaag gaataatgtt aaaaaacagc ttttcatgtt ttatgaatat
    26941 tgccttgtga ccgaggtgtt ggcaggagaa tactgtttat ttgaaaaaag ggctgcgaca
    27001 agacttttgt cacagccttt attttcatga ataggagtta cttttggcta cttttagaga
    27061 agtagctttt aaggagcaga taaagtcaag gcttttattt ctataaatga agtagtaaaa
    27121 tgaagaacag attttattac aggaaggatg ccgataattg tgatcattgt tgcaggaacg
    27181 atcggtgcag ggaaaagcac actaactgaa atgcttgccc aagatttaga aacaaaacct
    27241 ttttatgaga acgtggaaga taatgaagta ttgccgctat tttattctaa tccagaaaag
    27301 tatacttttt tattacagat tttctttttg aacaaacgct ttttggcaat caaagatgct
    27361 ttcagccatg atgacaacgt actagaccgc tccatctacg aagatagtat gctgtttcat
    27421 ttgaatgcag atttagggcg tgtttctgaa gtagaagtaa agcagtatga aggtctttta
    27481 gaaacgatgc tgaaggagtt agaagaaatt tcgccacaaa agaaaccaga tctactggtt
    27541 tatattcgtg tatcctttga aactatgtta gcgagaatca aaaaaagagg ccgggaatat
    27601 gaacagctag aacaagatcc tgaactctat tcctactata aagaactcaa tcgacgatac
    27661 gaagagtggt atgaacagtt tgatatatgt cccaaaatag tgatcgatgg ggacaaatat
    27721 gatttcgtag ccgatccggc atgtggagaa caaatcgtac aagaaatcaa gatgcgggca
    27781 aagaaaatga cagaagaggc agatgcccag tcgatccgct acactacaaa taacccgaaa
    27841 gcaaagggaa tctttacaac taaataatat aaaaactaag caaattgctc actcgcagat
    27901 atttctctgt gagcagcatc ttgcttagtt tttttgattc attgagatca aatagtgaac
    27961 cgattcgtat gataaaattg ccataaatga aatgtctgag tgagggccat gatcaaaaat
    28021 aaaatggaaa cgatcaaaat cgaatgggcg ccaaacagat gatgacagaa aacaaggaca
    28081 atagctcctg cgataaaggc aagaacgaca cttagatagt ggaaaaatcg atcaaaatct
    28141 gctttttctt tagaaataca ataatcatat aagttcgttg ctaccccttt taaatttcca
    28201 gttgtaaata aattagtgta ggcacgcccg ttgattttat caaaagctac ccactggatc
    28261 gatgcagtaa aagcaatcaa aatcgttact aatgcgggat gctcatggac gggtgtcaaa
    28321 ctaacaaaga aaaaaatcac tgcttcataa tatagaacaa acaagcgcca gaaatgtttt
    28381 tctttttttc taaaataatc aattagatac tttgttagaa gaatccctaa gaaaaagaaa
    28441 agggtagaca atactttttt accaacactt agccattctc cgtcaaatgc ttggatgatc
    28501 gatagaatga tattgccagt ttgagcagag gcaaaagcac catattggat ataagtataa
    28561 ctgtccatgg ctcctccgat aaatgtcagc aaaagtccta ttgtacgccc ctcgtgaaag
    28621 gaagtctgca tttttttcat caccttttat gaatattttt gtcttcctta tcttagcata
    28681 aacgattgaa aaaaaggaaa aattttcaga aaactaaaat catatcgctt cccattttca
    28741 tttttagaaa ggtctgttag tataaagaaa aaatagacaa cgaaacttta aataaccaac
    28801 ttgaaataaa caaattcaaa gaagaaagat gggaaggaaa tcaatcagtt gaatgaaaaa
    28861 gaaattctcc gattacaaaa agaattgaaa cgctgggaga acaagtataa aaagcttcaa
    28921 caagtctttc ggctacaaga agaaatcatt gctacctatg aagcggctta tggcgaagca
    28981 cccggactta ttgaggaaga agtacaagta aaagaaagtg aaggaaacag ctctttcttt
    29041 aaaatgatgg agaaacgttc ctataatcgc ttcaatcaag aacaaaaagc agctatcaca
    29101 tatgatatga agaaacatct tcgaatcata gcaggagctg ggagcggcaa aacgcaaaca
    29161 atctgtgcaa aagctgttta tctgatgacg caaaaacaag tagacgaaga acgcatacta
    29221 atgatcactt ttacaaggaa cgctgcaaat gaactaaaaa aacgagtaga taatttcagc
    29281 cagagaaaaa ccgctgtcca tatcggaaca ttccatagta tcttttttag gatatacaat
    29341 gaaatttgtc gaaaatttcc tgaagtagca atgcaaggaa tacaaggaga cttttcaaag
    29401 gatactgcac aaaaagtgaa tgctgttttg caacagctga tacgtaaata taatctttat
    29461 atatttgatc aatatggaga aaaaacgatt gcttctcggt tagactattg gcagaatatg
    29521 aacctttcag tagaagaaat ggttcagtta gttaaagaaa aatatgattc cattgataag
    29581 aatgcacgac agccgatatc agaacgactg tatggattgc tcacggaact ccaagaacaa
    29641 aaaagaagcc agcaattgct agagttcaat gatgtactcc aaaatctgaa aagcgctttg
    29701 gagaatgagg agatccagca ttatatcggc caaaaatatg attatctatt cattgatgaa
    29761 tttcaagata ctaatccact tcaatgggaa atcgtgaaac ggatgacaaa ggaaaacagg
    29821 atcaagttga ttgttgttgg agatgatgat cagagtatct atggattcag aggatcagag
    29881 ccagcataca tcaagaactt tgaaaaagaa tatcctacca aaactctttt cttattaact
    29941 aattatcgtt ctcgggcagc tattgtccaa ggagcaaata gattgatcag ctataataaa
    30001 aatgatcgga taccaaaggc gatgattcca gcacaaaaag aagcaggtgt tatggaagcc
    30061 tatttgtttt ctgatactaa agcagaaagt cagtggctaa tcaaacaaat tcagacattt
    30121 attcaaaaaa atgggaaata taaggaatca atcatattat accgatcagc ttcacaaacg
    30181 cagcagcttg tccaatctct gctgaaaacg aatattcctt ttgtactgga agcagatagt
    30241 ccatatgaag gtatctttgg tatcaagtct ttccaacatt tctatcaaaa aattgttcat
    30301 tggcaaacag cactgaatct gcaaaagaaa aatcaggcct accagcaatt gctccggcag
    30361 ctgatggcag actgctacct gaaaaaaagt gaatgcgatc aatacttttc tcctaaatat
    30421 caaaatatag ccgttgcaga ttatattttg tcgataagcc ccaatttaaa atcgaaatct
    30481 gcagaaatcc gagagtttga ggaagcgttg cgtaaaacag accagcatca agaaatctat
    30541 tcgcttgttc aaagctatct tcatctgcca cgtatagcta aggaaatcga ccagagtgat
    30601 caagaatgga tacgagaaga agtaaagcag catgctactt tcagaagact ccaagcctta
    30661 tcgcaagaaa cccaagcgac agctaaagaa ttaaaaaccc gcttgattgc ttacagacaa
    30721 ggaaaacttg atgcactatg tatccagagc attcacaaaa gtaaaggtct tagttaccga
    30781 aatgtctttt tgattggttg taacgaagga agtttgcctt ataacggagc gacagaaaag
    30841 aaagtaatcg actttaatga aataaaagca gaaccagtaa cgacgataga agaagaacga
    30901 agactatttt atgttgcgat gactagagca agaaatcgtc tgtatctatg tgtacctcaa
    30961 atgaaaaatt caagaaaatt gaaagtatcg agatttatca aagaaaccgg gagtcgtgtg
    31021 aaaaaaggaa agtgatagaa gagagggtgt gacagaagtg gtcagtttca agaaagaaga
    31081 aggaatgtac gaaaattgct tctcaaattt ttgtgaaatt tcagcttatt ttcgaagaag
    31141 ctatttctgg aacacagttt attcggaaaa gggagg
//
LOCUS       DAZKLA010000025        29383 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000025 DAZKLA010000000
VERSION     DAZKLA010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.025"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..893)
                     /locus_tag="ACG85N_000909"
     CDS             complement(45..893)
                     /locus_tag="ACG85N_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0223703.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1117..3057)
                     /gene="thrS"
                     /locus_tag="ACG85N_000910"
     CDS             complement(1117..3057)
                     /gene="thrS"
                     /locus_tag="ACG85N_000910"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFQ0223704.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3394..3573)
                     /locus_tag="ACG85N_000911"
     CDS             complement(3394..3573)
                     /locus_tag="ACG85N_000911"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFQ0223705.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3620..5443)
                     /locus_tag="ACG85N_000912"
     CDS             complement(3620..5443)
                     /locus_tag="ACG85N_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0223706.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5721..8000
                     /gene="helD"
                     /locus_tag="ACG85N_000913"
     CDS             5721..8000
                     /gene="helD"
                     /locus_tag="ACG85N_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFQ0223707.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8093..8740)
                     /locus_tag="ACG85N_000914"
     CDS             complement(8093..8740)
                     /locus_tag="ACG85N_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223708.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8919..9638)
                     /locus_tag="ACG85N_000915"
     CDS             complement(8919..9638)
                     /locus_tag="ACG85N_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0223709.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9769..10920)
                     /locus_tag="ACG85N_000916"
     CDS             complement(9769..10920)
                     /locus_tag="ACG85N_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFQ0223710.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11137..11877)
                     /locus_tag="ACG85N_000917"
     CDS             complement(11137..11877)
                     /locus_tag="ACG85N_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0223711.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11881..12204)
                     /gene="rsfS"
                     /locus_tag="ACG85N_000918"
     CDS             complement(11881..12204)
                     /gene="rsfS"
                     /locus_tag="ACG85N_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFQ0223712.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12261..12854)
                     /gene="yqeK"
                     /locus_tag="ACG85N_000919"
     CDS             complement(12261..12854)
                     /gene="yqeK"
                     /locus_tag="ACG85N_000919"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFQ0223713.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12838..13488)
                     /locus_tag="ACG85N_000920"
     CDS             complement(12838..13488)
                     /locus_tag="ACG85N_000920"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFQ0223714.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13498..13809)
                     /gene="yhbY"
                     /locus_tag="ACG85N_000921"
     CDS             complement(13498..13809)
                     /gene="yhbY"
                     /locus_tag="ACG85N_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFQ0223715.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13825..14934)
                     /gene="yqeH"
                     /locus_tag="ACG85N_000922"
     CDS             complement(13825..14934)
                     /gene="yqeH"
                     /locus_tag="ACG85N_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFQ0223716.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14931..15458)
                     /locus_tag="ACG85N_000923"
     CDS             complement(14931..15458)
                     /locus_tag="ACG85N_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFQ0223717.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15638..16423)
                     /locus_tag="ACG85N_000924"
     CDS             complement(15638..16423)
                     /locus_tag="ACG85N_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFQ0223718.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16416..17285)
                     /gene="accD"
                     /locus_tag="ACG85N_000925"
     CDS             complement(16416..17285)
                     /gene="accD"
                     /locus_tag="ACG85N_000925"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFQ0223719.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17286..18665)
                     /locus_tag="ACG85N_000926"
     CDS             complement(17286..18665)
                     /locus_tag="ACG85N_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFQ0223720.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18673..19092)
                     /gene="fabZ"
                     /locus_tag="ACG85N_000927"
     CDS             complement(18673..19092)
                     /gene="fabZ"
                     /locus_tag="ACG85N_000927"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0223721.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19117..19593)
                     /gene="accB"
                     /locus_tag="ACG85N_000928"
     CDS             complement(19117..19593)
                     /gene="accB"
                     /locus_tag="ACG85N_000928"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFQ0223722.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19596..20831)
                     /gene="fabF"
                     /locus_tag="ACG85N_000929"
     CDS             complement(19596..20831)
                     /gene="fabF"
                     /locus_tag="ACG85N_000929"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFQ0223723.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20850..21587)
                     /gene="fabG"
                     /locus_tag="ACG85N_000930"
     CDS             complement(20850..21587)
                     /gene="fabG"
                     /locus_tag="ACG85N_000930"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFQ0223724.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21599..22516)
                     /gene="fabD"
                     /locus_tag="ACG85N_000931"
     CDS             complement(21599..22516)
                     /gene="fabD"
                     /locus_tag="ACG85N_000931"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0223725.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22623..22847)
                     /locus_tag="ACG85N_000932"
     CDS             complement(22623..22847)
                     /locus_tag="ACG85N_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0223726.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22900..23862)
                     /locus_tag="ACG85N_000933"
     CDS             complement(22900..23862)
                     /locus_tag="ACG85N_000933"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFQ0223727.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23872..24312)
                     /locus_tag="ACG85N_000934"
     CDS             complement(23872..24312)
                     /locus_tag="ACG85N_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0223728.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24618..25583
                     /locus_tag="ACG85N_000935"
     CDS             24618..25583
                     /locus_tag="ACG85N_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0223729.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25640..25870)
                     /locus_tag="ACG85N_000936"
     CDS             complement(25640..25870)
                     /locus_tag="ACG85N_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFQ0223730.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25956..26843)
                     /locus_tag="ACG85N_000937"
     CDS             complement(25956..26843)
                     /locus_tag="ACG85N_000937"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0223731.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26932..28155)
                     /locus_tag="ACG85N_000938"
     CDS             complement(26932..28155)
                     /locus_tag="ACG85N_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0223732.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28172..29218)
                     /locus_tag="ACG85N_000939"
     CDS             complement(28172..29218)
                     /locus_tag="ACG85N_000939"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0223733.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
BASE COUNT         8146 a         6436 c         5062 g         9739 t
ORIGIN      
        1 cgttttgtta ttctaaaaaa cttatataaa aaatagtaag gtttttattt cgaatccata
       61 aaatattctt ggattgcttc tcgtgttttt ttcacttttt cgctaagctt ttcgttttta
      121 gccatataga aataaaaaat gatatcgatc gtagcaaatt gggccaaaag tgaattagtt
      181 gccgcacttc gattcgtgat ttcttttggt ctagatgttt gtacgagtac atcggctatc
      241 ttgctaagcg ggttatttcc aaattgcgtc aacgcaatga tttcgatatt catgcttttc
      301 gccagctcgg ctaatgcgac tacatctgca cttctacctg agtttgaaac aagccaaagc
      361 actttctttt tctcattgct gacaagttgc gggagcaaga cgtggatatc tttttcaaaa
      421 atgattggct taccgacacg agaccatttt tgcagaatat cttctactac taaagaagag
      481 gcgccaaccc catataaaaa aacagtctct gccgattcca aacactctac gactttttgg
      541 atcgtttctt cttctagaat atctactgtt tcttttatcg ctacttgtgc attgttacca
      601 agtttgtttt tgatagagta aaaggactca tttgcttcta tatcactaaa accttcatcg
      661 attccagtct gtagttctgt tgaaatcgtg attttgaagt ccgtcagact ttgaaaaccg
      721 atttttttgg aaaaacgtac aactgtcggc gcactcaacc cggaagcttc tgctaattca
      781 ttagctgtca ttgtagggac cttctcaata ttaccgataa tgtagttggc caactttttt
      841 tctgctgaag aataatcttc catcatactt tcaatacgac taattacgct cataagttcc
      901 tccacccttt taatgatagt ctatgtaagt ataccatatc agacccttat tcacggtcgt
      961 atactggata aaaaacaaaa aaacaatccg cccaacagaa tatcttatcc catcaatccc
     1021 acagattcta ttgagcatct gtgtctttga ggaatacctg ttatctattg ttcggattgc
     1081 tttctatcta ctactttttt acaggaaatt tacctattag ttttctctgc tgtaattttt
     1141 gacttctgct tccatagcat caataaagat attcaaatct tctacagctg tttctttact
     1201 tccataacga cgtacgttca ctgttgcatc ttccaattcc ttgtctccaa caactaattg
     1261 gtaagggatc ttttgtgttt gagaggcacg tatcttatag cccatttttt cattacgatc
     1321 atcaacttct acacgcaacc ccttcatttg caaacgttct ttgatttcat aagcataatc
     1381 gccatgtgca tcaacagaaa cagggatgat tgttgcttgt ataggtgcca accatgttgg
     1441 aaaagcgcct ttgtatactt ctgttaaata agcgacaaaa cgttccattg tagaaacaat
     1501 tccgcggtgg ataactactg gacgatgcgt attttccccg tcttctccaa catatgtcaa
     1561 atcaaagcgc tctggcaata agaagtctaa ttgtatagtt gataatgttt cttctgtacc
     1621 aagagctgtt ttgacttgaa catctagttt tggtccgtaa aaggctgcct cgccttctgc
     1681 ttcaaagtaa tctagttcta attcatccat agcggctttc agcattgctt gggctttttc
     1741 ccacattgcg tcatcatcaa aatatttatc tgtattgttt ggatcgcgat agctcaagcg
     1801 gaaacgataa tctgtaatat tgaagtcagc atatactgcc accatcaatt caagcgtacg
     1861 tttaaattca tctttgattt gatcaggacg aacaaatgta tgaccatcat tcaacgtcat
     1921 ttctcgtaca cgttgtaaac ctgacaaagc acctgatttt tcatagcgat gcatcattcc
     1981 taattcagcg atacgaattg gtaattcacg ataagaatgg atcgtgtttt tgtagaccat
     2041 catgtgatga gggcagttca ttgggcgaag aacaagcatt tcgccatctc ccatatccat
     2101 tggcgggaac atgtcttctt gataatgatc ccagtgtcca gatgttttgt acaactcaac
     2161 atctcccatt attggtgtat atacatgttg gtaacctaga ctaacttctt tgtctacgat
     2221 ataacgttcg atcgtgcgtc ggattgttgc cccttttgga agccagaatg gcaaaccaga
     2281 acctacttct ggggaaacca tgaacaagtc aagttctttt cctagtttac gatggtctct
     2341 ttcttttgct tcttcacgga gacggatata ttctttcaaa gctttcttgt caaaaaaggc
     2401 tgtcccgtat acacgttgca tcatttgatt gtttgagttt ccacgccaat aagcacctgc
     2461 cactgataat aatttaaata cttgtatacg gccagtggat ggaacatgtg gtccacgaca
     2521 caaatcgatg aattcgcctt gttgataagc agtaatcact tcttcttctg gcaattcact
     2581 aatcagttct actttgtacg gatcacttgc aaaaatttct aatgcttcct gttttgaaag
     2641 aacacggcgt tcgatcggca gattttcttt gacgattttc atcatttctg cttcgattgc
     2701 cggcaaatct tctgcagtta cttggttttg tccattatct gtatcgtagt aaaaccctga
     2761 atcgatagct ggtccaacgc caaagtggat atttggatag aggcgacgca tggcttgggc
     2821 catcaaatga gctgctgaat gacggactaa gccaagtgca tcttcatggt ctggtgtgat
     2881 gatctcaata cttgcatctt cctcgattgg acgagtcaaa tcaacgagtt ttccgttcac
     2941 tttaccagct aaagcttttt ttgctaagct ttttgaaatg ctttcagcaa ttgccaatgt
     3001 tgttgttcca gactcaaatt ctttgactgc accatctgga aaagtaattt ttatcataat
     3061 agtttcctcc ttgaaatgtt ttgattgatc agatcatcaa gctgaaaaac aaaaaaatcc
     3121 tagcatctct tgaaaagaga tactaggacg aaaagttcgt ggttccacct aatgttcaaa
     3181 cgcaaatgcg ttcctcaagc gttgtaacgg tccgaccgcc cttgcttcaa caagggtttc
     3241 gaaagtggtc atcctttggt tttctcctgg aaaagtttca gcctagcttt tcctctctta
     3301 tcgaagactc tcaaattcag ttggctttct catcaatcaa ttatgtgttt atttaaccac
     3361 taataaatag aaaaatcaag tgggaaacta aacttatttt ttccattccc catttacacc
     3421 gtatgttcct tttttcatct cattgatgat gacatggatg tgttcttttg gtgcctgcgt
     3481 gtttttatga acagcttccg tgatatcttt catcatattt tctaattgtt ccggactgcg
     3541 accttctact aattcgacat gtacgaatgg cataatgctc tctcctcttt ctgatcgttc
     3601 ttttttcaac tcgattctat catactatgt ttgtcttggg tactgaatca ggaaaaatgg
     3661 ctggatcttt ttttgctctc aaggcactca aaatagagac cgtatccacg acttcttgca
     3721 aaacagctcc gaataatgcg gggattagtc ccgtacttgc aattaacatc aaaaaaatac
     3781 aaataaagat gccaatcagt acagactgtt tcgctattct catcgtatct cttgaaatca
     3841 ctactgcttt tgctacacgc aagagatcat ctttcaagat gaccgcatct gcactttcac
     3901 tagcagctgt agaaccatga gcccccattg caataccgat atctgcagca gcaagtgctg
     3961 gtgcgtcatt cacgccgtct cctaccatcg caacaggtct ttgatcttta gggatacttt
     4021 ctaaaatggt cagtttatct tgaggcaagc tacgggcatg tacagaatct aattggactt
     4081 tttctgcaat cgtttgagca atggcagatc catccccggt gatcatcatc aaatttttca
     4141 catcaagttt tcttagctcc ttgattgtct ttgctgcttc tgggcgaatc tgatccgaaa
     4201 ataggatcgt tcctgcaaaa gatcgatcaa ttgttacata gattgctgta ttggacgttt
     4261 ctatttcttc agctgaaaca aaagaggcac gtccaatctt tacttcatga ttattgacta
     4321 ctgcttggat tccttgaccg gtcacttctt tcagttgact aacaggatac agttggctgt
     4381 ttttcacgtt gttcactaac gaacgagcta agatatggct agattcctgt tctgcactag
     4441 ctgcatacag aagtagctct tcttttttta ttccattgac tggctgaatc gcctctactt
     4501 ccaatatccc tttggtaagc gttcctgttt tatcaaaagc aatcgttttt gtacgtgcga
     4561 gcttttcaat cgttgttcca gtttttacaa cgatcccgtt tttacttgaa cggctcattc
     4621 ctgcaactaa agcaatgggc gcagctagaa tcagtggaca aggtgatgcc actaccagta
     4681 cctcagcaaa tcgtaaaggg tcttttgaga accaccaaga aataccaccg atcaaataag
     4741 caatcaacgt gaaagggact gcataacgat cagctaaacg gacaaatcgg gcaggctttt
     4801 cttttgattc ttcgactagt ttaataatca tttgatactg gctgtctgcg gcacgttttt
     4861 cagcgatcat ttggatcgat ccgtctccat ttaaagaccc tgacataatc tgatctccag
     4921 gtttcttggt ttccgggcgg gactcacctg tcaaagaaga ttcatcgacc gaagattctc
     4981 ctttggtcac tcgaccatct gcgggtacaa gttcgccagg cttgactagt aactggtcat
     5041 caatatttat ttcatcgatt actatatcct gcaattttcc ttctttgaat cgatgggcaa
     5101 ttcttggggt gttatctaac aaagactgca gttctttacc cgctcgttta ttcgcgtaat
     5161 cctctagact atctccgccc gtaagcatga tcaatatcat caaactagcc cagtattctt
     5221 tgacggctaa tgttgcaagg attgcggtaa tcgctaatac atctacgcca tatcttcctg
     5281 aacgtaacgt cttgatcatt tccaaaaaca tcaagaaggc gagaatccct ccagtaaatg
     5341 cgacaatcgc aaatgcaagg gccggctggt gaaaaacaaa ttcacttaat aaagctatcc
     5401 caccaataag caaagtaagt aaaaattttt tgaaatgtgt cattgttttt tctcctcttt
     5461 tatgtgactt agagaggtta gtaataaatt tccagctgcc tctttcatct taagcatagt
     5521 ccttgactag cactttttaa aacaataggc cttaattgaa aacaactatc actatcagcg
     5581 tttccaaaat tcatcagttc tacaaacaaa aatattcagc aaggattact ttttcgattc
     5641 atcagttaaa aagtaccttt tttcgaaaaa ctatgctata cttttaattc gttatttacg
     5701 gcggaaggag caaacatgcg atgaatgaga aacaactaga acaaacgcac gtggatgaaa
     5761 cgatcgaact aatccagatc gaacaaaata tgctggatac aaaacaacaa gaattgactc
     5821 aggaaatgaa tcattcgaca aaagacacag ctaatcacaa gattcgagct ggttccaatg
     5881 aagcttttta tgaatctgct gttgaatatc gccaacatga gcaggaattg cttttgaaat
     5941 accatactct tgaagcacag caaaaacgcc tgcaaacgtt gtctgtcatg aaagatagtc
     6001 cttattttgc ccgtatcgac tttaaagaag accgtgaaac agaaacatta tatctaggta
     6061 tcgcttcttt aagagatctg gcagaagaaa caatcgttat tgactggcgc gcaccaattg
     6121 ccaatcttta ttatgaaggt gagattggtc ccgcatttta tgaaactgaa acagatagaa
     6181 tagatgtcga tctgcagttg aaacgacaat ttaaaatcca agaaggacgt attttatcta
     6241 tggtagatac gtcggaagta atcaatgatg actttttgct agagatattg gatgaatcat
     6301 caagcaacca tatgaaaaac atcgtctcta cgatacaaaa agcacaaaat gcaatcattc
     6361 gggacacaac aagtaaagta atgctgattg aaggaatcgc cggaagtgga aagacatccg
     6421 ctttgcttca acgtattgct tttctgttgt atcataatcg caagtggctc gaggctgaaa
     6481 acgttcttct attttcaccc aaccatttgt tttctgatta catctcgact gtattgcctt
     6541 cattaggtga aagcggcgtg cctacacaga cattcaaaaa ttatttggtg cagctgctgc
     6601 cagagttttc tttactagat gaacagcaac aagaaagtgg ttttttgcta ggggaaaaag
     6661 atccgataca agtgatgaaa agcggattga ccttagtcga tcagattgat cgctatatcc
     6721 aaggcatcac aaactacggt cccttgttcc gtgatatgaa aataaatggc cgtacgatcc
     6781 tatcaaagga atcgattcgc caatggtata aagaaacaaa tgaacaatta cctttacatc
     6841 atagactttc tctattacaa acgaagctcc tgaaaaaatt aggcggtctg caaaaagacg
     6901 aaacacgtca gcaatgggtc aaagatctgg cggaagaaca gctccaagaa ctatacgcaa
     6961 cagatccaaa tcttgaatat acagaacaaa aggagcgttc attacgtaaa aaactggcca
     7021 accaaatcgt acgtaaaaga ttccgtcgaa tccatcgagg gatcatgaat tatcaatttg
     7081 tcaatataac gaaacaatat ctccactttc tacaaactgt gccactttct cttctaacac
     7141 gacataaaat cagcgagacc aggtggcagg aacatttgac aactgttcgt acacacttgc
     7201 gtcaaagaga attgcgtcaa gaggatgctg tactattctt tttactgatg cgccgaacat
     7261 atccagtgac ggttacacac aaagctcgct atatctttat tgatgaaatg caggatttcg
     7321 caccatcaca agtggcattg ctgcaagcct tgttcccaaa agcaaatttg actttctgtg
     7381 gagatctaaa tcaaaatgtc tttggaaatg aaacgatcgt tggatcattg gaagaccttt
     7441 tcgtggaccg tgaagtcact cgtttccaat taacgacaag ctatcgctcc acaaaagaaa
     7501 tcactgattt tgctaatcat tttattgtga aagaacatca agtagaaaca acagcaagaa
     7561 aaggagacct tcctttgctc atccaaggaa caacagatga tatcaaattg aattggttag
     7621 cagataccat tcgaacgact cagggaaaag cccggtattg gcgtacagca atcatagcta
     7681 aaacacaaga agaatgcgaa gctctttatg ctcaactgcc agacgagttg aaacaacaag
     7741 tccagctgat tttaaatgag agtgacttta tgaagcgttc cattatttta cttccagcct
     7801 ttttagcaaa aggtctggag tttgaccgag ttttcttgtg ggatgtagat gcagaacatt
     7861 tttcttctca gcaagacaaa ttgatccttt acaccatgtg tacacgtgcg atgcatgaat
     7921 tgaccctttt tacctccaat aatgaaagtc ctttgttaaa caatatggat atttcatctt
     7981 atgagaaaaa agtactgtaa gtaaaaagac aactgctatc attaatgtta gcagttgtct
     8041 ttttatttac aataataatt gcagaaaaag taattcaatc atttaaggtc atttattcta
     8101 tatataaatc caaatctgtt tcaggatatt ttaatttgac aagcgaaata atcattttca
     8161 gttcttccat gttttctgga tattctgggt caaatacctt gctatgacgg ccaatcccgc
     8221 cattgtcgat caatcggtaa attcgattcc attcaatgaa ttggtcaatt gattggaaat
     8281 tgctgattgg atagccgcca caatccaaaa tctctgatac acgaaatgct aggtatgcac
     8341 tatcgatcac tttatttttt ttcgtgtcta tcttctcatt tctttcgata agaatttttt
     8401 caggacgatg tttcaagctc cactctaata gtagaaagaa agcctccatc cgatactctt
     8461 cttcaaataa ccgaaaggct gaatcagaac gacgaaaaag cagcggaatc agataatttt
     8521 gtctataaat cagcattggt gcgcggtcca ccatacactc taaataacgg ttcagatacc
     8581 ctgtaccgac atagtttcct aatcgatagc cttcaacgcc gaatcttgcg tatttttgcg
     8641 ctaattttga acaatcagtt acgttcacag ctaactcttc tactttgcgg taaccgctta
     8701 agatacgttc aaaaggttct aaagtgattt ttgcttccat attcttcccc gtccatcttc
     8761 cacatacttc atatcatttc acatgatact tatgctctca tcataccaca ggttgaatta
     8821 gaaaagaaaa taaatcacac aaaaaagcaa gacaatctgt tagtgagatt gtcttgcttt
     8881 tcgaaaaaga ctgaatactt ttcgttaaaa ctggttgctt agtcttctaa tgccacatta
     8941 tggaacacat gctgtacatc ttctagatct tccagtgcat caatcatttt ttcaaatgtt
     9001 tcttgatctt ctggagatag tgttacctcg ttttgaggga tcatttggat ctcagcgatt
     9061 gaaaaatcag tgattccttt ttcttttaat gcttcttgta tactatggaa atccgctact
     9121 tcaccgtaaa cgatgatttg tccttcttct tccattacat cgcgaacatc gatgtctttt
     9181 tccattaggt attcaaggat ctcgtcagcg tcttctcctg caaaagcaaa gattgctgta
     9241 ttgtcaaaca tgtaagaaac cgctccggaa acacccatgt tcccgccatt tttcccaaat
     9301 gctgcccgta cgtctgctgc tgttcgattc acattgttcg tcaatgtatc aacaatcacc
     9361 attgagccgc ttggtccaaa tccttcatag cgcaattcag agtatgtttc atctcctgca
     9421 ccttttgctt tttcgattgc tcggtcaata atatgttttg gtacattata agttttagcg
     9481 cgatcaatga cgaaacgtag tttttggttt gcatgcggat caggatcacc tgattttgct
     9541 gccgcgtaga tttcaatccc aaattttgca taaatccggc tattattagc atctttagca
     9601 gttttctttg ctacgatatt tgcccattta cgtcccatat tttcacttcg ctttctgatt
     9661 tgatagtttc ataacgcaaa aacgtcctgt tccattatac ttgtgaatca cgattctgtg
     9721 aagctttttt tctttcttcc ttcattcttc tcatttttag tcaattatct atactcggat
     9781 tgggatttgc ccgaaattct gttccggaat attgcctccc aattgataga tttgatcact
     9841 tctaactaac agttccgctt cttgtgcctc tttttttccg attcgggaat aaatagaaat
     9901 ctcttttttt tcttttaaga atcttcttcc agcatcggta aatccaagta gtcgaagata
     9961 atttttattt tgttggacag gaatctcttg ctttttgata ttcagcaaga cataacaaga
    10021 taatcgttgg atccgtgtcc atgtatatct ttttgttttt acctttgaca caaaatcctc
    10081 aaaactatca gcagttgcag ctgctgtttt catgcgcgat tctattcctt cggtcatctg
    10141 atagatatct gtcagttcca gcaaagtaga agataacaac cgataacgca aatacggcca
    10201 ataagattcc caagtctgca gcgaacaagt tgaaagatct tcttctgtct gaggcggcac
    10261 aagtccagct attctttttc cttcttctaa cgcatggcga atggctgttg cactggctat
    10321 cctctgatct gtgatgattt tttcatgata gcctgcactt ttacgtcgga tcggatgtaa
    10381 ttccataggg tggacatact tggcattttc ctttgcataa cttaatccca agatatgatt
    10441 tggcgaagaa aaatcaagcg taatatcggg atacagactg cgaaaaacct cagtcatttt
    10501 ttgcggataa ctatacgttt gatcagacag ttcttgaaat gcctcttcta tcctatcttg
    10561 attctcttgg acaaagcgtc cgaaactgct gtaatcaaac gaagtctctt cttctgttcc
    10621 aaaacacaaa ctgtcacagt ccagtgtttg caagattttt actgcacctt tagcaaaata
    10681 gtcagctgac tgaaccgccc attcgacggg aagctccact actaaatcca caccattttg
    10741 taaagcttca ttcgcgcgat gccatttatc gatgatagcc ggttcgcctc tttgcaaaaa
    10801 attcccgctc attacagcaa tgacgacctc tgctcctgtt atttttcgtg ccatttccac
    10861 atgatagcga tgtccattgt gaaaaggatt gtactctacg ataatcccgc agcttttcat
    10921 gcgcatcctc cttcatagtt atcgataaaa aagaaataga gacaagtaaa aaaaggcaaa
    10981 aatcggaaaa aatacccgaa ctatataaac tattctcgca aacacaatac ttctgcgacg
    11041 aaaagctgca ctgcaagagc aatgcctata tcgttcggat atataattta tgttgaagat
    11101 ttattctttt cttgttaact cgagcaggca aaaccgctag tctttgtatg ctacaaagaa
    11161 ccagcgcttt gatttctctg atgctgcttc gtctgtaaaa tcagaaaatg cttcgacttt
    11221 cgaaaagccg ctgttttcca gcattcttaa ataactttcc aatggatagg tacgctcttg
    11281 atgtagttca tcttcacgaa taaatcgacc agacccttct tgttcttcaa cgaaaaaggt
    11341 aagaaaatgt tcgatgctgt gtttctcgct tcctggataa ctatcccaca aaaaagcaaa
    11401 attatcccct tgataatgat agctatattc tggaaaaacc tcatctactt gataggtgga
    11461 gtgtacgtca aataaaaatc ggccattttc ttctaacgct tgatagactt catcaaacac
    11521 ttgctgtacc tcttgtctat ttttcatata gcacagcgaa tcagaaaaac aggtaattgc
    11581 ctgatattgt ccgatttctg ataaatcgag catattgcct tccacaaatt ggatcgatgc
    11641 gtcttcttca tatgctcgct ggctggcaat catcaacatc tcttctgaaa ggtctaaccc
    11701 tgtcacttta tatccatctt ttgcaaaggc aaccgctaat gctccagtac cgcatgccag
    11761 ttccaaaata ttttttgtgt cttcttgtaa atgacgtttc gaaaaagcta accattgctg
    11821 ataaagtgtt tcgtccatca cttcatcgta aatgaaagca aatgtttcat aagccatatg
    11881 ttaatccaac cattcactaa gatcgactag cggagcatct gaccatagtt tttccagatt
    11941 atagaaattt cgttctggcg catggaaaac atgtacgatc acatctccaa gatcgatcaa
    12001 tacccatttt ccgccttctt ttccttcaat acgttttact tcatatttgt tttcttcttc
    12061 tttatcaatg atttcttctg taattgcgtt gatttgacgt tcgctatttg ctgaacagat
    12121 catgaaataa tctgccaaca aggaaacatt tcgaacgtct aaagcgataa tgtcttctgc
    12181 ccgttttgaa tctgctgctt caatcgcaat ttttaattgt tctttactat ctatgatgat
    12241 ttcctcctaa tatgagcagc ttattttttt gctacccagt aattatatgt ttcgattgtt
    12301 tttggataaa tctgttgttc ttgcgcaagc aaatgggcca atgtatgttt tgtttcaaat
    12361 gctacagctt cgtctagatc agcccatgca atggctcgtg catcttgtac cccaggaaaa
    12421 tctcgacctg gttcaatata gtctgctaca taaatgattt tgtcaaggag actcatctta
    12481 gcagccccag tcgtatgcac ccggattgca tgaagaatct ctgcatcggt gattcctaat
    12541 tcccgctcta caaacgaagc gccaacaaga ccatgccaga ttgcattatt ataattcaat
    12601 aactccggat caaaaccgtc tcttacgatt accattttga actcctcatc tggtcgttct
    12661 tttgcataat catgtgtcaa tgcagcaata ctagcttttt caggagaagc accgtatttt
    12721 ttggcaagag ccacggctgt ttcttctact ccaagcacat gcttgaagcg ttgttcgctc
    12781 attctcatct gtattttttg catcaggttt tctctagaat aagcagtata ttcattacta
    12841 tagtccatat tcatacaacc cttcattttg aatataatca attactttat ctggaacaag
    12901 gtagcggata gaacagcctt ctttgatctt ttgacgtatt ttcgtggaac tgatgtcgat
    12961 ctcaggtaca tctacccaaa ttacaggata tggagtatcc gttgtgtatc ctggtcgacg
    13021 tatacccaca aagttgacca tagaagtcag ctcatcgatt ttataccatt ttggcaaata
    13081 ttctaccata tccccaccga taataaagta ataatctgta tccggattgt tttgcgtcaa
    13141 ttccttcata gtatcatagg tgtaactttt cccaccacga gctaattcaa tcgtttcaat
    13201 ctgcaaaaaa ggattgtctt ccaccgctaa ttccaacatg ttgagtcgat gttttgcatc
    13261 aatcgtctgt ttctcatcaa catgaggtgg ctgataagaa ggcatcaaaa atacgcggtc
    13321 aagacctaaa ttccggccag cttgttcagc catcactaaa tgtgcaaggt gaacgggatt
    13381 gaatgttccg cccaagatcc ctacttgctt tttcttctct aagaattgag gcatttcttc
    13441 aggaaataat tgtgaacgaa caaatgtctt agcttgcgta ttcatcatgg tcactcctta
    13501 tattgctttt acttcttttg atagcctttg atacttttct tttgtggaag gtttgtataa
    13561 tacaataaca cgtccaatga tttggacgat ttcgcaatct atttcttttt ctaatacttg
    13621 agctgcttct tctgcaatct catctgtatt ttgaagtaac gatactttga tcagttctct
    13681 tttttctaaa gcttcttcaa tttgaacgat cattgctgaa ttgatcccac cttttccgat
    13741 ttgaaaaatc ggctgaagat gatgcgcttt gctacgtaaa aaacgctttt gttttcctct
    13801 taaactcata attttccttc ttccttaaat caatgctttg cgtttcaata catctacacc
    13861 ctttggcgcc cagcccgcaa ttactactgg cgctgttaca gtgatccagc ctagaccggc
    13921 aaaaacgatg tctgtttttt ctttcacaga aaactcaaaa cgaacaagct ccggaaatgc
    13981 ctcgacttca tcaggacgag gaggctgtaa taatccacct acatgttttt gataaaattc
    14041 gtctgctttt tccagtttcg ttcgatgaat atttacatca tttgacacat aagcaataaa
    14101 tgaagcacga ttgccgctga taaaatcaaa tcttgcaagt ccgcccaaga aaagtgtttg
    14161 cccttcattc agctgataaa ctttaggttt gatttctttt aacggtgcaa tgatccgcaa
    14221 atctttttta cctaagtagt gcgccatttg atggcgatgg ataattcccg gtgtatcaat
    14281 caaaaaatgc ccgtcatcta aaggaatctc gattttatcc aaggtcgttc ctggaaactg
    14341 cgaagttgtg attacatctt gtactcccgc tgtatttttg atgatttgat tgataagcgt
    14401 cgattttccg acatttgtta ctccgacgat atagacatcc cgtccatcgc ggtattcttc
    14461 gattttttct aatagttccg tcatctcatg agatttctta gcactagtta ataatacatc
    14521 tactggacga attcctgctt cgtaagcacg ttccttcatc cattgtgtta atttagtttt
    14581 tttcaatgat ttaggtaata tatccacttt gttcccaact aaaagtactg gattgtcacc
    14641 gacaaaacgg tgtagtcctg ggattagtga accattgaag tcaaaaatat ccaccacatt
    14701 gacgatcaat gcatcttttt gccctaattc attcagtaag cgcaaaaagt cgtcatccgt
    14761 caatgagaca tcttgggtct cattataatg gcgtaatctg aaacaacgct gacaatagac
    14821 ttccccggaa gctaaacctt tttccaaagc cgtttgcggc gtgtatccta attcaccggg
    14881 tttgtttgtt tgtataacgg caccacagcc gatacattgt aattcttctg tcattcgatt
    14941 ccacctcgcc agcccatatc agggtgtttt gctaatagat aggccataat tcttttttcc
    15001 caaaaacgat taaattgtgt cttccaacta tctgttgcga taatgggttt tacaaggatg
    15061 ctacgtatcc ctgcacgatt ggctccacgt atatcagtca taatctgatc gcctaccatt
    15121 actatctcat catctgctaa atccaattgt ttctttgcta tgttgatccc tcttgctaaa
    15181 ggtttcattg ctcgtgcaac atacaaaaga tcaaattttt caatcgctcg cgcgacacgt
    15241 tttgaattat tgtttgaaac aacaacaaca gggattcctg catttttcat ttctaaaatc
    15301 caagtaagca actcttctgt tccatctgga ttattccatg caatcaacgt attgtctaaa
    15361 tcggtcaata ctgctttgat gcctagattc tttaattggg caggtgtaat ctgatagatg
    15421 gcttctacca tccatgttgg tttgtaattt gaaaacatgc tgtctcctca tctgcaaaaa
    15481 atgaaggagc acataaagta ctcctcaacc ccgtattata ctgcatatca gcaaaaaaaa
    15541 ctacctctat actcgttcgt ctttcagaaa aagcatttct ttacaaaatg accaaaaata
    15601 aagattgatc acagaaaaat ttagaaacaa aaaacaacta atattggcgg aacctcttgt
    15661 atcgtttttc cagtaattta tgctgatcta atcgaacaag ttccgtcaat ctttcaatca
    15721 acgctttttt cataatgtgt gcgactttat atcgtggcaa ctgctcgccg ttgaacgttt
    15781 caggaataac tcgatcaacg attcctaact cgcgtagttc tggcgcagta attttcatca
    15841 actcagctgc ttctgcggcg cgactaccgt ctttccacaa aatcgacgca aatccttctg
    15901 gagaaagcac agcataaatc gtttcttcca gcatccagac ttcatccgct aaacctagag
    15961 caagggcacc gccacttcca ccttcaccaa tgatgatggc gatgatcggt acctttaaat
    16021 cagacatttc gagcagattt ctggcaatag cctctccttc gccgcgctct tctgctccta
    16081 caccgcaata tgcacctgct gtattgatca aggtaatcac cggtctattg aacttttccg
    16141 cttgtttcat cagtcgtaaa gcttttcgat agccttcagg atggggagaa ccaaaatttc
    16201 tgcggatatt ttcctgcaga tcccgtcctt tttgaatacc gatcactgtt accggtttgc
    16261 catctaatcg agcgattcca ccaacaatcg cttcgtcatc tgcaaataaa cgatcaccat
    16321 ggcattcctc gaaagaatcg aagatctctt caaaaaaatc caagcttgtc catcgttctt
    16381 tagctcttgc taactgaacg acatcatgtg ctgtcttatc cacgatctat ccatcctttc
    16441 tgtgtatgca attcaagaag cgtatggatt ttctgtttca attccatgcg gggaacaatc
    16501 tgatcgacaa aaccatgaga taataagaat tcagcctttt ggaaatcttc tggaagttcc
    16561 tgcttgatcg tctgttcgat gacgcgtctt ccagcaaaac cgatcaagct ttggggttct
    16621 gctagaataa tatcgccttc cattgcaaaa ctggcagtta ctcctccggt cgtcgggtcc
    16681 gttaaaacag ttagataaaa aagaccttca ttgctatgcc gtttgacagc agcagagatt
    16741 tttgccatct gcattagaga aaagattcct tcttgcatcc gtgcgccacc tgaagctgtg
    16801 aacaagacga ctggaagact ttcctttgtt gctcgttcga ataatctggt gatcttttct
    16861 ccgacaatcg ttcccatact tcccatgata aaattaggat ccatcactcc aataatgaag
    16921 ggaatcccct gaatggttgc tttaccagtg aggactgctt catgaagtcc tgttttatct
    16981 tgcatttttt taattttttc cgtatattcg ggaaatgcta gcggatcttt ggttacaaga
    17041 tccgtatccc attcttcaaa acttttttca tcaataatca aagctagacg ctgccatgca
    17101 ccgattcgaa aagcgtatcc gcaatgaggg catacttttt cttcaccgat gtctttcgta
    17161 taaaggatat gtttgcaatt tggacacttt gcccacatgt tatcaggaac agatggcgag
    17221 ttagcaggtt tgctctctct attaggattg atccgaatat atttcttttt cttaaacaga
    17281 gccatctatg cgcctcctat ctccgtttct ggtgtccaat tcggtaaaaa ttcttcttgt
    17341 aaaaatgctg tgctgtaatc acctgctatg actgcaggat gactaatcaa atccatctga
    17401 aattctgcgt tcgtgatgat tccttctgtg ataaattcac ttaaggctcg ctgcatcttc
    17461 attaatgctt caaaacgcgt atttccatgg acgattattt ttgcaatcat tgaatcataa
    17521 tatggcggga tcgtatatcc cgggtaaact gcactgtcta ctcgcagtcc catcccacct
    17581 gctggaagca gtaagttttg gatcttccct ggagaaggag caaaatggaa agcaggattt
    17641 tctgcattaa tccggcattc gattgcgtgt ccttggaata ctacttcttc ttgacacaca
    17701 ttcaaaggct caccggctgc tatctccacc tgttttttta ctaaatcgat tcctgtgacc
    17761 atttctgtta ctggatgttc cacttggatt cgagtattca tttccatgaa ataaaaatcg
    17821 ccttcttcat ccatcagaaa ctcgatcgtt ccggcatttt cataattgac tgctttagct
    17881 gctcgaacag cagcgtctcc tagtgcttct ctttttgttt gtgagatcac tacagaagga
    17941 gattcttcca gtactttttg attgttgcgt tgtaaagaac aatctcgctc gcctaaatga
    18001 atgacatgac caaatcgatc acctaagatc tgaacttcta tatggcgagc aggataaatg
    18061 attttttcca aatacatatc atcatttcca aatgccgctg ctgcttcttg ctgagctgaa
    18121 gaaaaatgct gtgggagctc ttcttttgat tgaactttcc gaatgccttt cccgccacca
    18181 cctgcagctg ctttcagcat cactggataa ccaattctgt ctgctatttc caatgcttct
    18241 tctactgtag ataatacacc agtacttcct ggaatgactg ggacatttgc ttcttgcatc
    18301 aattgacgcg cattgatctt atttcccatt gcatcgatcg ttgcactttt aggaccaata
    18361 aatgtgatat tgcactcttc acacatggag gcaaatcggc tattttcaga taaaaaacca
    18421 aatcctggat gaattgcttc ggcttttgtc acgattgcag cgctcagtac ttcctgaaca
    18481 ttcagatatg aatcaacggc ttttgccggg ccaatgcaga ttgcttcatc tgctaactgt
    18541 gtatgaagtg cttcggcatc tgcttctgaa tatatcgcta cggtttggat ccctagttcg
    18601 cggcatgcac gaatgatacg tacagcgatt tcaccgcggt tagctatcaa tactttagaa
    18661 aacatatttc acctatccaa tcataaaggt taattccgct tcggctactt ttttaccgtc
    18721 tactttagcg attcctttgc ctattccggc tgaagctttc actttcaata tctcaacttc
    18781 aaggtataag gtatctcccg gtgtaacttt cttacggaat tttgctttgt ccaatccgcc
    18841 gaaataagcc gtctttccct tgaattgttc aagagaaagt aatgcaactg ctcctgcttg
    18901 agccattgct tcaacaatca atacccctgg cataacaggt tcttctggaa aatgtccttg
    18961 aaaaaagggc tcattgatcg ttacattttt cttagcaacg atccgttctc cttcaaccat
    19021 ttcttccact cgatcaatca aaagcattgg ataacgatgt ggaatgattt cttttatttc
    19081 ttgtatgttc attgttttcc ctctttcctg ctttgcttat ttttgctgaa tcctaaagag
    19141 tggctgtcca tattcgacaa ctgactcgtt ttctacaagg atttctgtga tcactccgtc
    19201 ggcctcacta gttatctcat tcatcagttt catcgcttca ataatgcaga taacttctcc
    19261 tttggcaact tgatcaccta cttttttgaa agctggtttg tcgggcgctg gttgcagata
    19321 aacgattccc acgattggcg aaacaacctc gattccatca aatactgccg attctttttc
    19381 tttcattatt tctggagcag ttgttgcttg ttcaggtatg acattgactg gctccgaaac
    19441 tggcaacgtt tcgtttgttt gaaccatatt catgcctcga cttgttttat ttttattcat
    19501 atacaattca aattgacctt cacgcaaatc gaattccgtt agcgaggatt gatcaaattg
    19561 actgactaat tctctgattt cattgatatc caaaattatt cctcccagcg tttcatgcac
    19621 agaaccgcat tatgtccgcc aaaccctaat gaattactca ttgcataagt aaaacttgca
    19681 ggtttgcttt cgtttttgac aactgtcaag tcaatctcag gatcttgttt ttctacattg
    19741 atcgtaggtg ggataaattg atgttctaat gccagcaaag ttgccaaggc ttcgatcccg
    19801 ccggctgctc ccaataaatg tccagtcatc gatttcgtgc tgctgacgta agtatctctg
    19861 aaattttcac ctaatgcgat ttggattgct tttgcttctg cctggtcatt ggctggtgta
    19921 cttgttccgt gagcattgat atatccgact tgttccggct gtacaccagc ttcttccatc
    19981 gcaagcttca ttgctttaga tgcaccagaa ccatctggag taggagctgt catatggtaa
    20041 gcgtcacagt ttgcaccgta tccaacgatt tctccataga ttttggcacc gcgagcttgt
    20101 gcatgttcta aagattcaag aactagaacg cctgctcctt cacccatcac aaaaccattc
    20161 cgattttcgt caaacggaat cgaaccacga ttaggatcag ttgattctga gagtgctgtt
    20221 aaagctgcaa atccagcaat ccccatctca caaatcgttg cttctgttcc tccagcaaga
    20281 atgacatcag aatagccatg tttgatgtta cggaatgctt ccccaattga attcgttccg
    20341 ctagcacaag ctgttacgat agctgtacaa ataccttttg cacctacacg caaagcaata
    20401 tttccagctg ccatgttggc aatcgccatt ggtacgaaca ttggagaaac tcgttttgca
    20461 cctttttcat gtagacgaat aacttggttt tcgattgttg gaagaccacc gatcccagat
    20521 ccgatgatga cgccaaaccg gtcttgattc attttttctt tatctaaacc gctcatctcc
    20581 attgcttcta aagcagcgta gattccatag atcgaaaaca tatccattcg tttgctatct
    20641 tttttaataa aatatttatc gtaagggaaa tctttgactt cacccgctaa ggtaatccct
    20701 gtttctgaag catcaaattt agtgatcggt gcgatgccat tcgtcccctc ttttaagcta
    20761 ttcaaaaagc tgtctgcgtc atttccaata ggtgaagtga ctccatagcc agtaattacc
    20821 actcgattca tttgtttgct ctccttttct tatccgtgca tgaccaatcc gccatcgaca
    20881 ttgatgactt gtccggtgat atatggattt tgtgctaaga aaactgctgt ttgtgcaata
    20941 tcttctactt gtccgaaacg ctgcatcgga atttgctttt ccgcttgttc cttgactttg
    21001 tctgctaaaa cttccgtcat atcagttgtg atgaatcctg gcgcaatggc attacatgta
    21061 atgcctctag ttgcagcttc tcgtgccaca gattttgtta atcctactac gccagcttta
    21121 ctagctgcat aatttgcttg accaatattt ccaatcaacc cagatacact agagagatta
    21181 atgatggccc cttctcgctg cttcatcatt tttttcaata cgtgctgcgt catattgaag
    21241 gttccaatca aattgatatc cagacatttt ttgaaatctt ctgcatccat tcgcatcaca
    21301 agtttgtcat ttgtgattcc ggcattgttt actaacacat ggatcgatcc aagcttttct
    21361 tctgcttctt tgatcatttg tcccgctttc tcataatcag agatgtcacc ggaaactcct
    21421 acgcatttta caccgaatgc ttcgatttct tctatctttt cttttggaat ctctccgcga
    21481 ccattcaaaa tgatatttgc tccagctttt gcaaaagcta aggccatcgc tttgccgatt
    21541 cctcttgtac tacctgtgat aaatacattt tttcctttta catccataac gttcctcctt
    21601 atagtattgc tattgtttca tcaaatgtct gcttattctc aacacgcaga actggtattg
    21661 ttttatcgat ttttttcata aatccgctta atacttttcc ggggccgact tcgatgactt
    21721 gttcgatgcc gattgttttt aacttatgga tactctcata gaaacgaaca ggttgcatga
    21781 cttgctgttc aagtagtccc gcgattgttt ctttcttcat tatttccgtt gttgtgttgc
    21841 ttatgatagg aaaagaaggt tcagagaatt gaatctgact taggtccttc gccagttttt
    21901 ttgctgccgg ttctaaaata gctgtatgaa aaggcccgct gacatttaga gggatcattc
    21961 ttttgaaccc tttttctttt aatagtaaaa cagcttcgtc tactgctttt tcttcgccac
    22021 caatcacgat ctgctgaggt gtattataat ttgcaggaga tacgatgccg tacttgctag
    22081 cttcatgaca gctttcctcg atcgtttcaa tcggcgcatt catcactgca accattttcc
    22141 cgcttccagc tggggcagct tctgtcatat aagcgcctct ttttgctact aacgcaactg
    22201 cttcagtgaa ggatagcgca ttactagcta ctaatgccga atattctcct aaactcagtc
    22261 ctaaagctgc atctgggatg attccatgct ccttcaataa gcgataatac gcgatgctga
    22321 ctgttagaat agctggctgt gtatattgcg tctgatccaa acgttcattt tctgtaaagc
    22381 aaagttcagc catatcgtag ccgagaatct cacttgcttc atcaaatgtc tgctttacaa
    22441 cggcttcttc atataaatct tttcccatac cttgatattg tgcaccttgg ccactaaata
    22501 agaatgctgt tttcatgttt ttctccaata tatccagaat atatgtttga ttcttcctat
    22561 tcaaaaaggc cgagacattc ctatttcagg aaatgtccca gcccccaacc taattttaat
    22621 gattatgcag tttgtttttc tacgtaagtc actaaatctt gaaccgtttt gattccgtct
    22681 tctgtttcga ttttcacgtc aaattcatct tcaatttcat tgatgatttg gaataaatcc
    22741 aagctgtcag cttctaagtc ttcttggatg tcagtagtca atttgatttc ttcagtttct
    22801 tttcctaatt cttctgcgat gatttcttga attttttcga ataccataat aatatattcc
    22861 tccgatattg tttgcttttt atttttataa tttaacagtc tacaactcca tgagaatact
    22921 tccccatgtt aaacctccgc catagcctgt aaatattact ttctgctgtt ttcctaaggt
    22981 gatctttcca ctagaaactg cttcatccaa taagataggg atgcttgcag cagatgtatt
    23041 cccatattta tccatattgg tcaggaactt ttcttgcggc atctttactt ttcgggcaat
    23101 tttctcaata attctgaggt ttgcctgatg caaaagcaga taatcaactg gtttgtcttt
    23161 catgacttct tggatattgt ttgcaacgtc tcgtacggca aaatcaaaaa tatctctgcc
    23221 agtcatttgc aaataatacg aaccttctga atcttctgaa tagaacggat ttttattttg
    23281 ctggtagcca gcggtcaatg agctacttct ggtaccgtca gctgctaatt tttctttcag
    23341 caattttttc tgtggattcg cttctaagag aatcccacct gctccatcgc caaacaacac
    23401 agcagtcgta cgatcagacc aatcgattag cttagatagt gtttcaccac caatcaccag
    23461 tcccttagtt gagccgcagc ggatcatttt ttcagccatg cttaacgcat aaacaaatcc
    23521 tgagcacgca gcactgatat caaaagcgat agcatgagtt gctcccaatt tcccttgtac
    23581 ttgtgctgct acagatggtg tcgtgtaatc gggcgacatt gttgctacta aaataaaatc
    23641 caaatcttta gggtttagat ttttctgttt tatcaatttt tccgccacta aaatgcataa
    23701 atcagaagtc tcctgatttg ttacacatct tcgttcacga atacccgtcc ttgacgaaat
    23761 ccattcatca gaggtatcca tcatcttgga aagatcatta ttagtaataa tcttttcagg
    23821 aaggacgctg gctgttgcag taatcattcc aaattgttcc attaaatatc ctcacttata
    23881 gtcttgcaag aagtcgtgca agtttttcag tgcatccagc aaggcatgct gttcctccgt
    23941 agccattccg tctaagatgt tcttgaccat ttcacgatga aaatgctgat ggacccgaaa
    24001 tagtaacttt ccttttttcg ttagtcccaa ctttacaacg cggcggtcat cttcactgcg
    24061 gatacgttcg acatagcctt tttttactaa cttgttaata gcagtggtca aggtcccaac
    24121 agtaattgat agttctttgg ctacttctga agttgttttt ttcttgtaca tcccgattgc
    24181 ctcgatcgtg tgcatctctg tgattgacag atctttgaat tgagattttt tcagctcaga
    24241 ctcttcgatt gtcaaaatat cattgaagac actaacaaga taatcattta ctgtttttaa
    24301 atctggttcc atgttccacc gtccactaat tagtttgatt atcaaatctt ttgatgttca
    24361 aactatatct taactttttt caaaatgcaa ccttaaaaga tcattttttt actatatgag
    24421 ctttttttaa gattttactc atttaaaaca actgataaat cagcatttat aatttctttt
    24481 attagaaaaa tcatctatct cacatatttt gattatcaaa gtattttctg tgaacagaac
    24541 tatagaattc cctaaaactt gtccattatt tcaaaatttg ttatagtctt tttattcaaa
    24601 gcgtaaaagg agatcctatg ttaaaagaaa aaatgaaaac tctccccatg ctgccgggtg
    24661 tctatcttat gaaagaccga gatggtaagg tcatctatgt tggtaaagca aaacggctca
    24721 aaaatagagt aagcagctat tttcatcaga ataaacagca ttcaaaaaaa gtattacgta
    24781 tgattcatca tataacagat tttgatttcg tcgtagtaga cacggaatta gatgctttat
    24841 tgctcgagtg tcaattgatc caacattacc gtccttttta taaccgacaa atgaattatt
    24901 tcagtaatta taattatgta catattacca ataagggatt tgtattaaca gatactccta
    24961 cggctcgaac atacgggcct tttcgtctct ataaaaaaat gccgtctatt ttgcgtataa
    25021 tggaagaaac ttatcaaatg ccttggttgt ctgaaattag tttgttagct ttgcgagtcc
    25081 agcttcctga cctacaggaa atgacttttg aacagaaaaa aaaggaactc caagggctgt
    25141 ttcaaggccg aaataaaaag ctactgactt acttgaaaaa aagacaacaa catttcatct
    25201 atcagttgaa tttcgaaaaa gccggtatgc ttcaaaaaga tatcgaatta gttacttatt
    25261 ttattagacg gatccaggaa caaaagcagt ttttaagaac accatcgctc accttcagca
    25321 tgccattagc agcagatgag agtcagaaaa agcactacct cgtttgttat ggtcaacttg
    25381 cagaaacaat gattacctct ggtgacgttt ctcctgactt ctattatgaa aagaaagaag
    25441 ctcatttatc tttgaaacgt caattgtcaa aagaagaaat cgatcctgta caaatcttga
    25501 tcagttaccg caaaaaatta gaaaaagaac agtttgaaat tgaactgtta aacaaaaaag
    25561 aagctgaaaa gcaactaaat tagttgcttc tcggcttctt ttaggcatct tcaacttcaa
    25621 atgtcaatta tcctaaaatt tattggatta agtcatagat ttccatcgct acgatatcga
    25681 tattatcaaa ttcgtattgc tgatgagtat ctgtttcagt taattcaaat gtatcggtcg
    25741 ctttatcata agttactgta cacttctcta cgccttcttt ttcaaaacgg cgaacttgca
    25801 gttcattatc tgctgactcg accatagcct ccagtcgttt tatgattgcg actaattgtg
    25861 aatgtgtcat cggaggtcct ccttttctta tttctgttat attgtatcaa aagcatagcc
    25921 tacttgcatt gattttctct aaaaaaactc gttttttatg attttttttc tggtattttt
    25981 ccttcaggcc accaaagttt gatcaaagct tgggttccgt cattctcaaa acctttatct
    26041 gccaactctt catatagttt caatgcttgg gtagttgctg gcaatacaag atccattttt
    26101 tctgcttctt ctaatgcaat ctttaaatct ttgataaaat gtttgacaaa aaaaccaggt
    26161 gtgtaatctt cttttaaaat acgaggagaa taattgctta aagaccagtt agctgcactt
    26221 ccacctgaaa gcgtctcaat cactttcttt aaatcaagtc cgtttttttg tgcatagaca
    26281 agcatttccg tcattcctgt catcgtacct gcaatcatga tctgattagc catttttgta
    26341 tgctgacctt tcccagcaga accatgcaat gtaaatgtcg taccaaattc tttgaataaa
    26401 ggcagtactt gatcataaac ggcttcttct ccaccgacca tgatcgtcag tgtaccattc
    26461 tttgctccta agtcaccacc ggataccggt gcatctaacg catctgcgcc tttttctttt
    26521 gctgtttttg caattttttc tgccaggctt ggtgtacttg tagtcaaatc aactaaaata
    26581 tgtcctgaga tatctgcaga aaaaatcccc gactgtccat agtagatttc ttctacatct
    26641 ttggggtaac ctaccattgt aaagagaata ttactttgtt ctgctaccgc ttttggtgta
    26701 tctgcccaga tagctccttc tgttactaaa ggatcagctt ttgcttttgt ccgattatag
    26761 acagctactt cgtgtcctgc ttctagtaga tggcgtgcga cagcacttcc catgacgcct
    26821 gttccgataa aaccaagttt cattttttcg acctcctaaa aatcaaaaat ccttgcttct
    26881 atgctagtat acgctagaaa caaggatttt ttcataaaaa atgtttgaat tttatctttt
    26941 aaatgaattt aattttattt tgattcgttc aatcgagtct tcttcttctt ttcttcgcat
    27001 ctcttcttca tgataaacaa ccatgtcttg ataaaattcg atcatagctt cgccgaaatg
    27061 tgtagatgaa atatcataaa gtttagcttc taaaactttt ggatctttag gaacttcact
    27121 ggaaatataa tcaataagtg ctggtgcaaa gtcttcgtcg gtttcaaaag ttttccctaa
    27181 tgaaggatga tcgaacaatc ggttcaaata agcatttcct tccacgacac aaggtgttcc
    27241 ggctgccatc gcttctgtat aagtaagccc ttgtgtttct gaagtagaag cactgacaaa
    27301 ataatctgcc gctttataat aaagtgctac atcattgttg tccacttctc ctgtgaactg
    27361 cacaaattct tctaactgca gttctgagat caattcctct aactcttcta cataaggtcc
    27421 ttttcctacg ataactaatc tggcgttagg ataagcggag atgacctgag gaaacccatt
    27481 aatcaaggct tggatatttt tctcataaga aatcctgctt aaagaaagaa tcatcaaatg
    27541 gttttcctga agacctagtg actcccttaa tttgctgata tcctgctgag tgatatcaga
    27601 acgtttgaat ttttcaatgt cgataccagt aggaatcacg cgaagcggtg ctattacacc
    27661 atagctgcgt aatgtatcaa taacacgttc acttggacaa actacacctg tcgtatgatt
    27721 tgcaaataat ctagacaagt attttacatg ggattgcctg atcacttttc ctttaccgat
    27781 ataatgaagg tagtcttcat acatcgtatg gtaagtatgg atcagaggta ttttcaactt
    27841 cttagctacc atcttcccaa gtatccctgc tccgaattct gtatgggtat gaataagatc
    27901 tagttctagt tctttggcaa ccaaataagc ataccacatg cctctaacaa caactcttcg
    27961 atccttgaaa gaaataaatg gtacacttgg cattcgaatc acatcttttt ccaacttatc
    28021 cgcattagga tcggtcgttg taaaaatata gacttcatgc ccatgtcttt ctaattcttg
    28081 ttttaatgtt ttgatcgatg tagcaactcc gctgacttgc ggaaagtagg tatcggtaaa
    28141 aaagccgatt ttcatgtttt tcgcctccaa actagtcctt gacctgattt acttttttta
    28201 atttgctaga gactttttca ttcagtacac tttcgtatac atttttaagt tcttcaccga
    28261 ttttattgat acttcgctct tttgctgttt gataaccggc ttgtgtcgta ctcggtagtt
    28321 cttgattggt aatctgttgg atcagtcgga taaattcttc gtttgtcttc cccatatagc
    28381 agttcttgct atcttctaac cagccattat acactggaat atctcgaaca agtacgtttt
    28441 gtttgcttgc taaagcttct aaaacaacga tcccttctgt ctcttcataa gaagggaaaa
    28501 agaatagatc tgcacctgaa tacgcacctt ctatgatctc tccacgaatg taaccaggaa
    28561 acaggacatt ttcaggatga tcttctttta caattttccg gatgttccga ggaatcgagt
    28621 acatgggaac atgaccaaac caaataaatg tgtattcggg catcttctta gcaatttcta
    28681 cgaaatcaat gattccttta cgttcgaaaa acaaaccgac acaaatgatt actttttgat
    28741 ccggagacag tttgaaatat tctctgaact tttgttcttt cattggatca gggtgatatt
    28801 tctctaagtc gatcccattg gaaatagctt gaatcggcac acggatacca tagctttcca
    28861 acaactgttt tgaataaggt gttggcgtga tcagatagtc cgctttttgg tacaatccta
    28921 ccagatattt ttttacgata ggagaaagct ggttcgatcc gataaaggaa tttcggaaat
    28981 cttcctctgt agaatgcgca tgatagacaa ctttttttcc ttctcttctg gctttattga
    29041 ccatattatg actattgatt ccgtatgtat tgatgtgcag gatgtcataa tcttcttttt
    29101 catctagtgt ataagagata ccgacttccg tcagagcacg tttttgatga tcaagcgcac
    29161 gcccgattcc agattttgca agcatctttt cactttcaaa atataataat accttcacct
    29221 tacgtccccc ttcacttata tagtataaca tagcctacaa actgtcactc aaacttaagc
    29281 cctatttttg tctattctta ctgacaaaaa cgttttctta aaaaagaaca aaaaaactgg
    29341 cgtagctctt ttacaagcca caccagcttt tgatacgttg ttt
//
LOCUS       DAZKLA010000026        26600 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000026 DAZKLA010000000
VERSION     DAZKLA010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.026"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            172..1938
                     /locus_tag="ACG85N_000940"
     CDS             172..1938
                     /locus_tag="ACG85N_000940"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFQ0223734.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2040..3299
                     /locus_tag="ACG85N_000941"
     CDS             2040..3299
                     /locus_tag="ACG85N_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0223735.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3446..4747
                     /locus_tag="ACG85N_000942"
     CDS             3446..4747
                     /locus_tag="ACG85N_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0223736.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4750..5601
                     /locus_tag="ACG85N_000943"
     CDS             4750..5601
                     /locus_tag="ACG85N_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFQ0223737.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5614..6429
                     /locus_tag="ACG85N_000944"
     CDS             5614..6429
                     /locus_tag="ACG85N_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFQ0223738.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6580..7746
                     /locus_tag="ACG85N_000945"
     CDS             6580..7746
                     /locus_tag="ACG85N_000945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0223739.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            7976..8623
                     /locus_tag="ACG85N_000946"
     CDS             7976..8623
                     /locus_tag="ACG85N_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0223740.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8707..9651
                     /locus_tag="ACG85N_000947"
     CDS             8707..9651
                     /locus_tag="ACG85N_000947"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFQ0223741.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9786..11237
                     /locus_tag="ACG85N_000948"
     CDS             9786..11237
                     /locus_tag="ACG85N_000948"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFQ0223742.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11258..12094
                     /locus_tag="ACG85N_000949"
     CDS             11258..12094
                     /locus_tag="ACG85N_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0223743.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12094..12753
                     /locus_tag="ACG85N_000950"
     CDS             12094..12753
                     /locus_tag="ACG85N_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0223744.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            12879..13568
                     /locus_tag="ACG85N_000951"
     CDS             12879..13568
                     /locus_tag="ACG85N_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223745.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13574..14530
                     /locus_tag="ACG85N_000952"
     CDS             13574..14530
                     /locus_tag="ACG85N_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFQ0223746.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14574..15086
                     /locus_tag="ACG85N_000953"
     CDS             14574..15086
                     /locus_tag="ACG85N_000953"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0223747.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15342..15890)
                     /locus_tag="ACG85N_000954"
     CDS             complement(15342..15890)
                     /locus_tag="ACG85N_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0223748.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(15979..17160)
                     /locus_tag="ACG85N_000955"
     CDS             complement(15979..17160)
                     /locus_tag="ACG85N_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0223749.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17396..18904)
                     /locus_tag="ACG85N_000956"
     CDS             complement(17396..18904)
                     /locus_tag="ACG85N_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFQ0223750.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19063..19254
                     /locus_tag="ACG85N_000957"
     CDS             19063..19254
                     /locus_tag="ACG85N_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223751.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19457..20374
                     /locus_tag="ACG85N_000958"
     CDS             19457..20374
                     /locus_tag="ACG85N_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0223752.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20367..21440
                     /locus_tag="ACG85N_000959"
     CDS             20367..21440
                     /locus_tag="ACG85N_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223753.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21588..22106
                     /locus_tag="ACG85N_000960"
     CDS             21588..22106
                     /locus_tag="ACG85N_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223754.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22087..22479
                     /locus_tag="ACG85N_000961"
     CDS             22087..22479
                     /locus_tag="ACG85N_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFQ0223755.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22555..23490
                     /locus_tag="ACG85N_000962"
     CDS             22555..23490
                     /locus_tag="ACG85N_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0223756.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23623..24402
                     /locus_tag="ACG85N_000963"
     CDS             23623..24402
                     /locus_tag="ACG85N_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0223757.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24395..24712
                     /locus_tag="ACG85N_000964"
     CDS             24395..24712
                     /locus_tag="ACG85N_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0223758.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            24956..26566
                     /locus_tag="ACG85N_000965"
     CDS             24956..26566
                     /locus_tag="ACG85N_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0223759.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
BASE COUNT         8590 a         4717 c         5346 g         7947 t
ORIGIN      
        1 caggaagtag attaaaactt ttaaaagttt taagtctatc tatttggcaa gtttaaaaaa
       61 taaaaattcg gtatcaaaaa actgttaccg ataacatttt tgaaagcgtt gtcttttgtt
      121 tctctatcac ttatagtgat gacgtcatat taaaacacaa ggaggaaatc aatggataca
      181 gctgcaattt atcatcgacc ggaaagtgaa tatgcatttc tatatacgaa tacaagattt
      241 cgtattcggc ttcgtactag gaaaaatgat atcaaaaaag tatatgtgct ttgtggagat
      301 ccatatacca taacaactga aaaatggtat caaaaacaac gaccaatgaa aaaatgtcta
      361 agtaccaatg ttcatgatta ttgggaaatt gaagtgacga gcgaaacaag gcgcttacag
      421 tatgcgttcc atgttgttgg ggaagataac acggactgtt tttacggtga tcaaggaatt
      481 tttccttatc gagaaaatgt gattactgaa ccaaattttt attttcgaat accttatttt
      541 catcaaatcg atcgattcac tattccagaa tgggtaaaag aaacagtatg gtatcaaatt
      601 tttcccgaaa gatttgcaaa cggggacaaa gcaaatgacc ccgaacacac tttaccttgg
      661 ggaagtaaag atccagaccg agaagacttc ttcgggggag atttgcaagg tgtgagggat
      721 catttggatt acttgacgga ccttggcgtc aatggcatct atttttgtcc catatttaaa
      781 gccacatcca atcataaata cgatacgatt gattattatg aggtggatcc cgcttttggc
      841 gataaaaaac ttttaaaaaa cttgattgat gaagcacata aaagagggat acgtatcatg
      901 ttcgatgctg tattcaatca tatgggtgtc cattcaccac agtggcaaga cgttctcaaa
      961 aacggagaaa aatcgatcta taaagactgg ttccacatcc gttttttccc agtagattct
     1021 tatcagatga ctgacttacc agaaactgct gaaaatatcc catatgatac ttttgctttt
     1081 actccattca tgccgaaact aaatacagca aatccagaag tgcaaaaata tttgctggat
     1141 atcgcaactt attggataaa agaattcgat attgatggat ggcgtcttga tgttgctaat
     1201 gaagtcgatc atcatttttg gaaaaaattc agagaagcag ttacagaaat caaaccagac
     1261 atctatatcc ttggagagat ttggcattcc tctcaagctt ggctgcaagg ggatgaattc
     1321 catgcagtga tgaattatgc attcacagat tcgattaaag attattttgc aaaaaagaaa
     1381 atcacagctt cacaaatggt cagtggaatg aatcatcagc aaatgcttta cagagatcag
     1441 gtgaacgaag gaacattcaa cttattagat tctcatgata ctgccaggat cttgaccctt
     1501 tgccaaggta acaaagaatt gatgaagtct gtgatggcct ttatgtttct tcaaaaaggg
     1561 tctccttgta tctattacgg gacagaaata gggatgactg gtgaagatga cccggattgc
     1621 cgcaaatgta tgatatggga gaaggaggaa caagatctag agctattcgg ttttataaaa
     1681 gaacttgttt ctctaaggaa acagttgtct aagattattt ctgaaggttc tactcagtgg
     1741 ctaatagtat atgatcgtga agataagctc tatttcacac gggaactaga gggacagatc
     1801 atctatgttt actttaatca gtcaaaagaa ccttgggtag tagaacagga aaatgaggtg
     1861 attcttagtc aaaattgtca gttgttagaa gacgggaaag cagagatacg acaatacgga
     1921 tttttgatca tgtgttgaaa actgttaccg gtaacaacaa gaaagcgttt gcaaatgaag
     1981 atttgtgccc attataataa gggtgtaaca gcacgttgaa tacacaggag gaaatgaaga
     2041 tgaaaaaaag aagtgtgaaa cagttgggtt tagggttaat ctctttagga atcttaggcg
     2101 gattagcggc ttgtggcaat ggtaactctt cagcagaagg ggaagccgat gaaggaaaaa
     2161 cgttaacagt ttccgttgat tctggatata aagattatat aaacgaaata aaaaacactt
     2221 tcgaaaaaga aaacgatgtg aaaatcaagc tagtagaaaa ggacatgttc gatcagttgg
     2281 aatcattagc gttagatggg cctgctggaa agggacctga cgttatgatg gcagcatatg
     2341 accggatcgg tgcgttagga cagcaaggac atctggcgga agtaaaactg ggaaatgaag
     2401 cagcttacga tgaaacagac aaagcccaag tgacttatga tggaaaaatc tacggagaac
     2461 ctgcagtaat cgagaccctt gttttgtact ataacaaaga tttagtcgat acagcaccat
     2521 caacgtttaa agatttggag aatctatcca aagacagtcg ttttgctttt gagtcagaag
     2581 caggaaaaaa tacaggattt ttagctaaat ggactgactt ttattattcc tatggtttaa
     2641 ttgcaggtta cggtggctat gtatttggtg atgatggcac aaatccttca gatatcggtt
     2701 tgaataatgc aggtgctgta gaaggaatct cttatgcaac agattggttc aaaaatgttt
     2761 ggccaaaggg aatgcaagac ataaaaagtg caggagactt cgccagccag caattcatgt
     2821 ccaataaaac tgcagcaatc attgacggtc catggcaagc gcaaacatac aaagaaaaca
     2881 atatcaatta tggtgtagcc aaaattccta ctctaaataa tggacagcct tatcaaccgt
     2941 ttggcggagg aaaaggatgg gtagtcagca attatgctaa aaacaaagac ctctctcaaa
     3001 aatggcttga ttatgtaacg aatcaggaaa atcaagaaaa attctttgaa atggtaaatg
     3061 aaatccctgc aaaccaacaa gctagggaaa cagcaaaggc taaaaacgat gaattaacaa
     3121 cagctgtgat tgaacaatat gaaacagctc aagcgatgcc aaacatccca gaaatgggcg
     3181 aagtttggac aggtgcagaa aacttgatgt tcgatgcagc atcaggttca aaaacaccaa
     3241 aagaatccgc agatgaagca gtaaaaacaa tcagtgaagc catagaacaa aaatactgat
     3301 taaaatgata accgtccatt tttataaaaa atggaacagg tgcttcagat aaaagtggca
     3361 agagcgagat ggaaaattat cttgctcttg ttgccattat cttaaaagca gtttgcggtt
     3421 tagcaagttt agagggggca ttcagatgca gcaagataaa acaagcaata tcaacgaaaa
     3481 aaaaatcacg ctttgttcac ttattccagg gatggggcag tttgtaaatg gccaagcttt
     3541 taaaggtatc gtttttttcg ccatattcgt cctattcatt ggtcagatgc ttctgggcgg
     3601 atttcatgca ttggtaggat tggttacttt gggaagtata ccaatggaag atcattcatt
     3661 atttattttg attgaaggca ctttgcaatt catcattaca atcattttct tgatttttta
     3721 cggtttgaat cttttagacg cttatcgagt agcgaagatg cgtcaagagg gcaaaaaaat
     3781 caatcggacg atgaaggaag tcatacataa tataggtgat gaaggttttc cttatctatt
     3841 gacacttccc gcttatcttc tcatgatttt tacgatcatt ttcccagtcc tagttacatt
     3901 gttcactgca tttactaatt atgacttcaa tcatattcca ccagccagtt taattgattg
     3961 ggtaggggct aaaaactttt ttagtatctt ctttttaagc tcatatcgta atactttttt
     4021 tgcagtcttt tcgtggaccg tgatttggac tttatgtgca acaactttgc aaatcaccct
     4081 tggtattctc acagcggttg ttgctcacca gaaatttatt aaattcaaac gtattttcgg
     4141 tatcattttc ttattaccat gggcggtccc agcttttatt actatcatga gtttttcgaa
     4201 tatattcaac gatagcgtag gtgccatcaa tactcaagtg attcctttca taaaccagct
     4261 tccttttgta gatatcagtg caatctcttg gaaaacggac ccgaactgga cgaaagtagc
     4321 aattatctta gtgcagggct ggttaggatt tccctatatc tatgtgatgg ttacaggaat
     4381 cttacaatcg atttcagaag acttatatga agcagcaaaa atcgacggag caaatgcatg
     4441 gcagcgcttt tcaaacatca cgcttcctgc tgtcttccta gtggcagcgc caacatttat
     4501 tacacagtat acggggaatt tcaataattt ctccatgatc tatctgttta acaatggtgg
     4561 tcccggaagc gtaggcggag gagctggttc tactgatatt cttatttcct ggatctataa
     4621 acttacgact ggaacttctc cgcagtattc tatggcttca gcaatcacgt tgattatctc
     4681 cgtcatcgtt atctgtgtgt cattgctggt atttaaaaag acaaatgcgt tcaatatggg
     4741 ggattaacaa tgaaaaaatt tcattcgagt gctcgattcc aaaaacgact ttctcacttt
     4801 tttacttact tttttctttt aatactgtca gtcattatca tctatccgct tttgatcaca
     4861 gcaagtactg ccttcaagtc aggaaatatt gcagctttta gtttagatct ttcaagtgac
     4921 tggactttag gaaactttac tcggctattc agtgaaacac tttacgggac ttggtataaa
     4981 aatacattga tcatttctgt cctaacaatg atcgtacaag tgactgtagt aactttggcg
     5041 ggctatactt atagtcgcta tcgttttatc ggccgcaaat caagtttgat gttcttttta
     5101 gtgattcaga tggtaccgac tatggcagca ttgactgcct tttatgtcat ggctttctta
     5161 ttagaagcgc tcgataagta ctggttcttg acaatgatct atataggcgg tggaattcca
     5221 atgaatactt ggctgatgaa aggatacttt gacactgtac caatagattt agatgaatct
     5281 gctaaacttg atggggcagg gcatttcagg atttttttcc agattgtatt accgcttgtc
     5341 aaaccgatgc tcgccgttca agcattatgg gcttttatgg gaccttttgg ggatttcatg
     5401 ttagctaagt tcctgcttcg aacacctgaa aatcagactg ttgctgtcgg cttacaaaca
     5461 tttatttctg atgtgacaaa ccaaagagtc tctctttttg ctgctggggc aattttgatt
     5521 gcactaccga tttgtctatt attcttcttt ttacaaaaga actttgttac cggcctgaca
     5581 tctggcggaa caaaaggata ggagcagagt atcatgcaaa catttccgtt aaactatttt
     5641 tccgcattac gtacgccaac tcaagtgttt gcgggacgca aactattatc atggcctaaa
     5701 tttttcttga tatttgtatt tttagtttcc ttgatggtca tgcctgtgac tcttttttat
     5761 gccaatcaga tccaagcgat tccattagaa caatttctat ccgttcattc gctgatcgat
     5821 gaacaaggaa cccaaaaatt ttctgaactt gaattatctg aaactgggtt acaagcatcg
     5881 caacaaacga tagctgtgac ccctgaaatt ttggtaggtg tgtctttatc tgagaaacaa
     5941 caatccgatc atggaacttt tattgatttt gagaaggaac aatgggtgat ccaacaaaag
     6001 gataaatctg gtatacgccg ctatacgatg aattatagcc catctttcca accagattct
     6061 gtacgtacgc ccgaagattt ccaacgattt ttagaaagag aattttatgc cagtaatcgt
     6121 ccaacaatca ttttatctta tagtttaagt cttggtcttg tcctatttgt catgacgagt
     6181 ctcattttat tcggggcttc ctttttcttg tggatgacaa gaaaaagcca actttcatcc
     6241 attcatacat tcaaagagag tgcaaatttg atgctgaatg ttatgggaat aggttccatg
     6301 gcagcagcag ttgtagggtt tatccacttt gactttatct tgatgctaag tgtccaaaca
     6361 acgataactg tcttgctgct tttatgggtt tttgtcaaaa caagattcaa agataagcgt
     6421 gtcgaatgac aagtttaagc gatttatgcg acaatgaatg tgaaaaaaat gaaaaataaa
     6481 gtagtttttg aaaaatttct gaaaaaatta aacttcttag ttgattcttt tcttttttag
     6541 tattaacgta atagggtagt ttttttaagg aggaaaatca tgttagagaa aactttatac
     6601 aatcaattat tttcacgttc tttctcgctc cctgtcgaag taaattattg ggacggtaca
     6661 acgaaacaat acggagatac ggacactcct ccccaaatca aaatcaatat ccatgaagaa
     6721 atcccaatga aagagattac aagtaacgct tctttggcac taggagaagc ctatatggat
     6781 catcgaatcg agatcgaggg aagtatccaa gcattgatca acgatgctta tcagcaagca
     6841 aacagctttt tacgaggaaa agattatcta aaatggctgc caaagaaaga aaaacatacg
     6901 aaacaaaaaa acaaagaaga tatccatgcg cattatgact taggtaacga cttttataaa
     6961 ctttggctag atccaacttt gacttattct tgtgcttatt tcaatacacc tgaagatacg
     7021 ttggaacaag cacaaatcaa taaagtccat catatcttag acaaactctt tatcaaagaa
     7081 ggcgaaacgt tgcttgatat cggttgtggc tggggaacat tgatgttcac tgctaccaaa
     7141 gagtataatg tcaaggcaac tgggatcaca ctaagtgaag aacaatacga tttgattacc
     7201 aaaagaataa aagaagaaca tttagaagat aaatgccgtg tattattaat ggattaccgc
     7261 gaactaaaag gcgaaacgtt tgatcatatc acaagtgtag gtatgttcga acatgtaggt
     7321 tctgaaaatc ttgaaggtta tttcaaagtg gtaaaagatt tattgaaacc aaaaggaaca
     7381 gctttgatcc atggaatcag ccgacaacaa ggcggtgcga caaatgcttg gatcaataaa
     7441 tatattttcc ctggcggtta tgtccctggt gttactgaat tagttgataa tattacaaaa
     7501 aacgatttgc aactgattga tttggaaagc ttacgtcgtg actaccaatt gacattagaa
     7561 cattggacaa agaattttca tcaagtccaa gatcaagtca cgaaagaaat gggtgattcc
     7621 ttctatcgaa tgtgggatct atatttacaa gcttgtgcag cttcatttga atcaagtaat
     7681 atcgatgtga ttcaatattt gcttgttcaa cctggaaaca atgacatccc aatgcatcgt
     7741 gcataattga ggcaaaagta ctgtttaaag actatttaaa aagaagctaa gttttatgat
     7801 gctccagaag tgaacttttt tgtctaactt ctaggattgt ctctaaagct tagtttcttt
     7861 ttctttatat tttcataata aaagtttggt tctttaattt ttaaacatga cagaaaattt
     7921 tttcttcgtt atacttgtaa tagaatacta ttttataaca aaggggatcg aatcaatgaa
     7981 aaaaactgtt atttatacta gtttatttgc agctttgttt ttagctgctt gtcaatcaaa
     8041 taccaatcaa ggcacatctg ggaatacgtc ttccagtagc gaacagcgct cttcacaggt
     8101 atcggactcc tcaacaaaag caagcacatc ttcgtctagc cagacaaaaa gttctagtga
     8161 agcaagtact cagtcagata tcaaagtgtc tgcagctcgt gcgatcgaat tgttccaaga
     8221 taaataccca gatactgcaa ttactagttt ggaattagat agtgattggg gatcttactt
     8281 ctacaagata gaaggtgtcg atgatcaaaa agaatacgaa gcaagaattg atgctcaaaa
     8341 tgaaaaaatg gagacagaga atccagaaac tctagatcga gatgaacaaa atggtcaaaa
     8401 gaaaaaagaa gatggtctgg atgttgataa attgatcacg attgaggaag cagcaaaaat
     8461 cgccgtcgac aaagtaggaa gcggtacggc aacagattgg gatttggata aagatttagg
     8521 tactacttat tgggaagtca aagtaaaaga cggcaatcaa agtacatcag tcaagatcaa
     8581 tagccaaaca ggcgaagtac tgtcaactga acaagatgat taagaatttc ttcggaaaac
     8641 aagagttgat ttttctttta gtcatgatac actagacaaa ataacttgtt tgaaggagcg
     8701 atttacttga gtttagaaac gacctttgcc aatcatatct ttgccaatcc tttgatgaat
     8761 gcatctggtg tccactgcat gactacccag gaattagatg aactggctca ttcagaagca
     8821 ggagcattta tcactaagag ctgtacgatc aatgaacgaa aaggaaatcc cgaaccacgc
     8881 tattttgatg ttcctttagg gagtatcaat tctatgggac ttccaaactt aggtttttct
     8941 tattatctag agtatgctct tgcgtatgaa aaagtgcagg aaaaccaaaa tcaaccgtta
     9001 tttttttcca ttgccgggat gagtgtccag gaaaacttgg aaatgcttga gaaaatagaa
     9061 aaaagtgggt ttaatgggat cactgaatta aatttatctt gtcccaatgt gcctggaaaa
     9121 ccacaactag cttacgattt tgaggccact tatgaaacac tgaaagaagt attcagcatc
     9181 ttttctaagc cgctaggaat caaattgcct ccttattttg attttgctca ttttgatcag
     9241 atggctgata tcttgaatca gtttccattg acctatgtca atgctatcaa cagtgtgggc
     9301 aatggtttgt atatcgatac agaacaagaa gcagtagtca tcaaaccaaa agaagggttc
     9361 ggcggcattg gcggagagta tatcaagccg actgctttgg cgaatgttcg cgctttttat
     9421 acacgtttga aaccagaaat ccaaatcatt ggtacaggtg ggattcggac aggacaagat
     9481 gcatttgaac atctgctatg tggcgcaagc atgttacaga ttggtacaga gcttcataaa
     9541 gaagggccgg aaattttttc tagaatcata aaagaattaa cgcagattat gtcagaaaaa
     9601 ggatatacgt caattgacga atttaaaggc aaattacgca ccatttcata gcaaagagac
     9661 gaaggtcatg attgatcttc gtcttttttt ataaaaaaca ctcgtctgtt cattcctgtg
     9721 caagcgtact cattgtatgt tagacgaact tgtgggaaaa taacagaggc aaaaaggagg
     9781 ataatatgaa tgatgcaaca gaattagcaa aagagcttaa agaaaaagaa tttagttttc
     9841 aagaactagt tacggatata cagaaaaaaa taaagaagca aaatcatgaa ttgaatgcgt
     9901 ttgtcgcgct tgaaccattc gagagtaatt cccacgagct attgattccc cagtcagaaa
     9961 aagggccatt ggctggtatc ccatttcctt taaaaatgct cggtcaagaa aaaaagggat
    10021 ggctagctac ttcaggatct cgtttatttg aaacacaccg agcttctcat acatctaatt
    10081 atgtgcggca agcagaagcg ataggtctag ttccttttgg acaaaccaat gcgccagagt
    10141 ttggtttcaa aaatattaca gacccagtca tctatggccc agcaagaaac ccttggaacc
    10201 ttgaatgtac accgggcgga tcaagtggag gagctgctgc tgcagtttca tcaggaatcg
    10261 ttcctttagc tggtgctagt gatggcggtg gttctattag aatacccgct tcgttttgcg
    10321 gattgattgg gctgaaacct tcgcgaggga cgatgccagt tggtccatat gcttggagag
    10381 gatggcaagg agctgctatt gatttcggct tgacagtttc tatgagagac acggaggctt
    10441 tattttatgg gatgagaagt atccattccg gtgcgccata tcaagtatcg ccagttgagt
    10501 ggcaaacaca tccaagaaaa aaacggttga agatagcttt ttgtgttgat tcaccgatcc
    10561 actcacagat tagtgaagag gcagtacttg ctgtaaaaaa agctgtagat ttccttggaa
    10621 aagaagggca tgatttgaca gagattcctt acccactaga tggtcgtaaa ctgatcacgt
    10681 cttattatca gatgaatggc gcagaaacag cagcaatgat ccgctcgatt gaaacaggca
    10741 tccagcgagc tgtcagaaaa gaagaacttg aacccatttc ttggactttg ttccaatatg
    10801 gtcagaagct ctctgccgct acttacatcg attctctgca tgtatgggat gaagcagcaa
    10861 ttgtcatgga aaacctacat caagaatacg atctattcct ttcaccaacg actgcattta
    10921 ccgcccctaa aatcagcgaa gatttgcaaa gtgatgcgat aagagcacag atggaacaca
    10981 tcagtgattg ttctgaagta gaaggtttag acgttgtttc ttccatgttt gaggagagct
    11041 tgaagatcac accttataca cagttggcta atctgactgg acaaccagca atcagcttgc
    11101 ctactcattt gacaaaagaa aacatgcctc taggaatcca atttatggct gccagaggaa
    11161 gagaagattt gctatttcaa atcggctatc tatttgaagc aaatcatcag ttccatttac
    11221 caaatccatg gaaatagcag aaacaaggag aagaagaatg aagaaaaaaa gattgtttgc
    11281 tctaataact agtgcctttt ttattttaag cgcttgcggt acagctgccg ctaatcagga
    11341 agataacagc tggagtaatg tagaaaaaaa gaaagaatta gtagtagcga cttctggcac
    11401 actgtttccc gcatcttatt ataatgatca aaatgaatta gttggttatg atgtagattt
    11461 ggcaaaagag gtggctaaac ggttaaatgt caaaatctcc tttaaagaat acaacgtgga
    11521 tggacaaatc acttctgttt caaaaagaga atcagatttt gctgcgaacg attttggtat
    11581 cacaaaagaa agagaaaaaa aattctccct ttcagagact ttcaaatatt catttgacag
    11641 tatgatcgta cgaaaaaaag atgattcagg aatccactca cttgaagatc ttaaagggaa
    11701 aaaagcagct ggcgaaccca atactagcta tatgcgttta gcggaaaaac acggagctaa
    11761 attagtcaca tatgacaatg ccacgaatga tcagtattta agcgatgtag caaatggtcg
    11821 aacagatgtt attctaaatg attattatct gcagaaaatg gccgtatctg ctttaccaga
    11881 tatccctgta aaaatactgg agaacgttta tttcaatgaa aacgaaagtg gttttttatt
    11941 gaaagaagga aatgatgctc tgaaaaagaa aatcgatggc gtattaaaag agatgaaaga
    12001 agatgggacg acaaagaaaa tatctgaaaa atattttcaa actgatgttt cagtcaaacc
    12061 gaaagaaacg atggctgagt cggtgagtga gtgatgtact ttcccctaat tgatttttcc
    12121 ctcatgtgga cttcacttcc tttcgtactg caaggcttgg tatacacatt agggattggc
    12181 ttcgtgtcgt ttcttttagg aaatttggta gggttgcttt tgacagtttt aggtcttttg
    12241 gattggctgc ctctgaatgt atttattcgt ttttatttat cgttctttag aggcattccc
    12301 gcactagtcc tgctgtttct gttatatttc gggttacctt atcaattaag tgccttaacg
    12361 gcttcagtca tctgctttac gattaccagc agtgcattta ttggtgaaat ctatcgagga
    12421 tcactagcag gcgtttcttc aggtcagtgg gacgctggtt tagcttctgg tttttctttc
    12481 tttggggtat tgaaatatat cgttttgccg caagcttttc gaatctcagt accggcactc
    12541 agtaacgtag ctatggatct agtgaaaggt acttcgttag cagcaacaat cacagtgcca
    12601 gaaatatttc aaaaagcaaa gatcataggc ggtcggacat tcgattattt ttctctctat
    12661 attttagttg cactgattta ttgggtcatt tgtatcggga tcgaaaaagt gcaacagtat
    12721 ttagaaaaac gttctatcca tcagtcaatc tgaaaaatag aaatggtgct agcgtggctg
    12781 gcaccatttc tattttttgt ttcttgtata ttctgcctag tattttaaaa ccatttcatg
    12841 taaaatgtaa atcgaaaata tgttcggagg tagaaaacat gaagaccccg ctaaccatcg
    12901 aaatagaatc ctcgttatac caatattgca tcgagcaagg cggcttggtt gtagaagaag
    12961 tcacgatgcc tgatgaccaa ggaattgtag atacgcttag ctgcttgaca aaatttgatg
    13021 gaagccgaga atggcgatgc tatgagctga aagtgacaaa agcagatttt cgctctacag
    13081 ccaaactgtc atttgttgga cattataatt attttgtttt accaagtaaa ctatatgaag
    13141 aagtaaaaac tgaaatccct acaggaatag gtgttttagt gtatcgtccg tacacggttg
    13201 aagaggagat gcctgcctct ggtacatttc tgattgcgaa aaaagcagca aagaaagagc
    13261 ttgctgtacc ggaagaagct ttgacaaacc gcttcatggc ttctttattc agggaagtaa
    13321 gaaaagcaaa gcaaatggag cgaggcgtaa aatattttcc aaaagatcaa ttgtacaaag
    13381 aattgaaaag acgttcagaa aatcaagatc cattttctaa cgaacgatat tatgaacggt
    13441 ttttagagga aatagctagt caggcaatca atgagttgca agaagaagtt atcgcgcttc
    13501 agcatgatta tgaatatttg aggcaacaac agcagatccg tcggttgcct acagagccat
    13561 tggagtgaaa aatatgtact atccttattt aagaggaaaa caatttgacc tattagcatt
    13621 aaaagaagca ctgtcgcgag gattgctctc caataaaatc caaccagtga tcgaaccagt
    13681 acgtgattcc gctacgctta aaaatgtgat cgaattattt caaaaaaagc accatccaat
    13741 cgctgtgatc caaaatcccc aagtaggaca attcaaatta ttcgatcagc atgtgcacag
    13801 ttggacagtc aaagaaaata gctctgtcgt ccctgcacag atcttaacgc cagaaaatct
    13861 cagtgaagta ctggattcac ctccagcgtt tcttgttttc gatggacagc actacccaaa
    13921 agatacagag gtgtggaaac agttggcagg agttgattcg aaatttttga taccagatac
    13981 tagtcgcttt cgcatctggc tgcccgaaaa caaaattgtg atccgtgact cttttcaaac
    14041 gagaaagcat gttgaaagtt atgcagataa aacagatgac ttttttagtg atgactatct
    14101 ttttttccac gcagacggat atattggatt ttctgatttc acgatcgaag gtagccgtta
    14161 tttcgataaa gggttcccta gccgagcgct tgctatccat ctaacgtata ttgatgctta
    14221 cggcaatata cgcgtgaaac attttgtctc tgacagtaac gactctgcta aagaccaagc
    14281 attgaagttt cttgaagcag gagataaaat gaaaaaatgg atcatgcgtc accatcatca
    14341 actcttgatt acttcaggaa tgattgaatt attggctctt taccagcaac aaaaattccc
    14401 tggactggga gtgttgaaaa agtggtcact tatgcatcat ttggaactta ttagtcacct
    14461 gctagatcat ccaaaagatt ggcttaaata ttattcaaaa gttcaggaac tatatgaagt
    14521 gatccaatga ttagataatg taaatgaaaa aggaatagaa aagaggaaac tggatgtata
    14581 ttcggcaaac aaggaaagaa gaagtagaag aagtggtaga aatcattaat gccgcagcgg
    14641 atttcattgc ttcgcaaggc tctcctcaat ggcaaaacgg gcagaaacca acaaaagaaa
    14701 cgattttgaa cgatattgaa agaaaagaaa gctatgtatt aatagtggaa gatcaaattg
    14761 ctggaacagc tgcattagtc tcaggcgtgg atcccgttta cacaaaaata gatggtagtt
    14821 ggaaagaatc aactggtcct tatttatcga ttcatcgtgt cgctttagca gaaagtactc
    14881 gaggaaaaaa actagcaaaa ccgttcttag aacatttgtt gacgattgca gccagcaaaa
    14941 atatccaaga tatccgtatc gatacttatc cagctaatga accgatggaa aagaccatct
    15001 attctgctgg attcacttat caagggatga ttgaatttcc ttttgaacac ggcgaaagaa
    15061 aagcatatca aataatcatc aattgactat aaaatcatct gagcaaagat aaagacatta
    15121 acactactga aaaaaacaat catatgatag ccaagaggtt gtaacagaag tggccagctt
    15181 caaaaaacaa gaaagaattt ccgaaaatgc ttctcaaatt tttgtggaat tttagcgtat
    15241 tttacgaaat agctgctttc tgaaactccc ttcatcgaga ggtcgtgaca tagcttttgt
    15301 cacgatctct cggcttatcc tacgagtatt attcgataca tttatatcaa taatcctttt
    15361 tttattttat ctgcagcttc ttggaacgct ccatcgtttg acacgactaa aagtgagggg
    15421 atagctttca aacgtttcaa ctgtgctaaa tcttctacat aaaccgccag tctggctgaa
    15481 atctgttcag atgaatctcc acgttttttc aggcgcgatt gcaatgtttc tttagagatc
    15541 gtaataaaga taggacggac taccgtcccg aaatactcat ataaatgcca aaaaccattt
    15601 gctgtcaaaa cggcgtaaca atttcctact gtcagtttct ttttaatttc tgctttactg
    15661 gaaccatatt tatgttgagc atattggtcc cactctgcaa actcatttcc cttgatcatc
    15721 tgattgaatg tattgtcaga aacaaaataa taatcctctt gatctttttc cccaggtctt
    15781 ggtgatcgat tcgtgtaagt aatgatcttt tgttccggct gaaaaatagc ttctgcgagt
    15841 gaagtttttc cagaaccaga aggaccgata aatacatagc aataattcat tgtcatttct
    15901 cctttgatga ttgaacaagt gaaaaagctt tggaaaatat ttccaaagct tttaaaagta
    15961 actttgcttt tttgtatttt ataccagcaa gccagtcaca atagcactta aaaagctgac
    16021 taaagtcgct gttagcaaaa ttttcaatcc gtgttttgcg acaagatttc ctttttcttc
    16081 atttaatccc ttcatcgcac cagaaatgat cccgatagaa gagaagttgg caaatgaaac
    16141 gaggaagaca gatataattg ctgttgtacg ctcagtaaaa tggaaattac catttgctaa
    16201 ttctgtcata gcaacaaatt catttgtaac cagtttagtc gccatgatac taccggcatc
    16261 cactgcttct ttccatggga ttccgctgat gaaggcaaga ggggcaaaga tgtaacctag
    16321 gatctcttgg aaagtgatgc cgaatatccc gtggaagata gcattgatca tggcaatcaa
    16381 agcaacaaaa ccgataagca tagcagcaac cgtgatcgcc acatggaaac catcaagaat
    16441 gtattctccc aacatttgga aaaatgtctg ttctttgttt tcttcaatga tcagctcatc
    16501 ttctttttct tctaatacat agggatttac aattgaagcc agaatataac cgccaaataa
    16561 gttcaaaaca agggcagtga tcacatattt agaatcgatc agtgccatat aagatccgac
    16621 gatcgacata gaaactgtag acatagccga tacactcatg gtgaacaaac gtttctcaga
    16681 taggaagggt aattcctttt ttatggaaat aaaaacttcc gattgtccta aaatagcaga
    16741 agctactcca ttataagatt ccaatttgcc catcccgttg attttactta atcctagacc
    16801 aacagctttc ataaataatg gaagaatctt gatatagcgt agaataccga taatagcaga
    16861 aataaataca attggcatca atacggaaag aaagaaaggt gcacttcctt tatttgctac
    16921 accggcaaaa acaaagtcaa caccttgccc agcaaaagca agaagatgat caaacatttt
    16981 tgccaataca gaaacaaccg ctgtaccaaa agttgtacgt aataatacga aaccaagaac
    17041 aagctgtaaa acgaacatca caattagtgg tttcaatttg attgcttttt tgttactgct
    17101 gatcaaaaaa gcaagcccta atgtgacaaa taaaccaata atacctaata aataactcaa
    17161 ataatcactc ctattccaaa ttccgtttta ttttacctgt tttgcaaata aaatactaat
    17221 ctttagtgaa attttcatta cttttttaga gtgaaacgca ttcattttcc attagaaaat
    17281 aaagcgaaca aaataaaagg tttaaaaagt gtaacgtctt agtaaagtct ataaaaaaac
    17341 gaaactatcg attaagctaa tagtttcgtt ttcaacattt ataagtaaac tttttctatt
    17401 ggtaatatag attttcggaa gggtagacag gtgcgcaggt aatatcgatt cctagttttt
    17461 tcaatgtttg ttcatcattt ttattgacca tcacagtaga gtgggcttgt gctccctcaa
    17521 gttcagctag tttatcataa gcaacttgag cgattggatt cgttactgca ctgattgata
    17581 aagcgatcaa gacttcattg atgcttagtg catccaaaga ggaatgaagt gtctttgatt
    17641 tcaatccttg tattgtttca agaactaatg gtgacaataa aggaatgtca tctgcgatat
    17701 gggctaaatg ttttactgca tttaagataa ctgccgctga cgcatccatc aatgaacttg
    17761 tacgcccggt aatgatgact tcatctggta attcgattgc aatcacagca gaaggatctt
    17821 ttgtaggact ttgcgcttga atcttatctg catagtcatg ggcagggact accacgtttc
    17881 ggtcttcagg acgtaaaccg acttcttcca taatcagttt gattcgatta actgcttctt
    17941 cgtcgatcaa tccttttttg aagtcgcatt ctgtttggaa ataacgacgg atgatttctt
    18001 gtttagaggc ttcttggaca acggtatcat ccacgatccc aaatcctaca cgattcacac
    18061 ccatgtcagt aggagattga taaacagatt ctttaccagt aattttttca ataatacgtt
    18121 tgattacagg aaatgtctca acatcccgat tatagttcac agctacttta ttgtaggcat
    18181 caaagtggaa ggaatcgatc atattgacat cttttagatc gactgtggct gcttcataag
    18241 caatgttcag cggatgtttc aacggaacat tccatactgg gaaagtttcg aacttggagt
    18301 aaccagctgc atgtcctttt tggctttcat gatacagttg gttcaagcaa gtagcgagct
    18361 ttccacttcc tgggccagga gctgtcacta caacgattgg tttagttgtt tcaatatact
    18421 cattttttcc aaaaccatct tctgagacga ttttttctac gttgactgga tagtcttcga
    18481 tttctgagtg tttatagacc ttgatgtttc gtctttctag tttattgata aagactttcg
    18541 tggcaggctg tccactatag cgagtgataa cgacactgtt gattaataag ccataaccac
    18601 gtaattcatc gattaaccgc aagatatcca tatcataagt aatcccataa tcaccacgga
    18661 tcttattacg ttcaatatct ccagcataga cgcaaatcag tatttcagct tgatctttca
    18721 atttttgcag caatttgatt ttcgcatctt cttcaaatcc tggaagtaca cgtttcgcat
    18781 gcttgtcgtc aactaacttc ccaccaaatt ctaaatataa tttgtcatag tggttcactc
    18841 gttccaaaat atacgctgac tgctcttcaa tatatttttc tgaatcaaaa ccaatttttt
    18901 tcactaggtt ccctccttaa gtactaaagc attttttcat ttttttgagg attcgtcaac
    18961 tggaagagcg aaattcctca aaaaaacaaa gaaaatggtt taaaccaact tgtctagata
    19021 gttattcatt ggtatgatag tttttgataa ggaaggtgct acatgaaaaa aagacagaaa
    19081 aagaaaaacg catataaaca ctatatccgt tcgattttta ccggttatga aagaatgctt
    19141 gaagatcccg aattggaaca attgacgttt acttatctaa acgaagaaac gcagctaact
    19201 cgtgatgagc atcagcgcat tcatttcaca acaagagatc tgcctagtaa ataaaaaata
    19261 agataaaggt catcggattc gattctgtcc tatcagatgg aaagactagt tgatcgaaaa
    19321 aaaggctgta ccaaattttg gtgcagcctg ttttatggag cattgcccgc tcttttttat
    19381 aacaattttt tacagaaaat ggtactattg aagtattaat aagtgaaaaa cagatgagta
    19441 agaggaggca agtagtatga aagagccaga tacaaatgca aattatgagc cagacgaact
    19501 agaggagacg aacgcggatc aggatacagg acaagaatcg tcttcttttt acgatatcaa
    19561 agaaaaagca agcatgaatc tttcccaatt attagaagac ttacaggctt ttgaaaaagc
    19621 tgtccgcgaa gaagatatcc ctgagatcta ccgtttatac aatggccgat tgaatgaaga
    19681 attaaaaaga agctcaaatg aaaatcacga gatcgatcaa ttgctgatgc gtaagatcca
    19741 tgatcgtttt ttacaggaat ttccttttat ggaacaagta gaaaacatct cgccaacgat
    19801 gagctattac aaaatcggca cttattacca tgatcggccc acggtcggta ttgatgcaag
    19861 tctgccagaa atttttgttt tgcctaagat cgatgaagag tgggagaaat attcccaacc
    19921 ggagtcaacg gactatgaca aaaaaatcaa tgagctagac gcaaaggtga tcactgctca
    19981 gacagaaatt gaacgtttag atgaacaaat aaaagaaatc aatcgacaaa tgactgattt
    20041 gaacgacaat aaaggattct tgaatcgtaa aaaaatcgag gaagaaattc aagaattaga
    20101 gaagaagaag caagtactat ctaatgaaaa attaggctgg cttccttata ttgagacgcc
    20161 tgaaacaatc cagcagcaaa aagaagcatt gaagcaagaa gcaagagcag accagcttcg
    20221 tgcagccatc gtagaaaaag aacaaaggca aatcagacgc tatttcggta gcaaagaggg
    20281 attcggtcaa gcaatccatg aattcttgat gaattatctt ggcaatgaga atcctaataa
    20341 tcaatctaat gaaggaggtt ccgagtatga atgaacacga tgaagcagaa tggacaaaaa
    20401 atgaagaaca aacagaagat catgaagaaa cgaacacttc attggaagaa caaacaagta
    20461 atcctctgga tgacacagaa gaaatcccta tgctagagga aacaaaaata catgaaaaag
    20521 aaaaagtaac tccgacgaat acgaatactg cagaaaacac ccatgcttac gaaataactg
    20581 aaaatcccgg gtatttagaa ggaaaagagc aagatcatca agatatcgtc agtttgattg
    20641 aaacagcgga agcagaatta gcggatttac gtatgagaaa agatttgatc gaatcagatt
    20701 ttccacaatt attagaatca tatgatcgtt ttatattgaa tgaagaacca atctccacga
    20761 tcggaaagac aagcctgatc gaatacttca cgtatgaatt actgaaaccg ttgaatgata
    20821 ttggtctgga acggtcaaat tcagcttatg acacatggaa aacaagacat ttttcgatca
    20881 attatacgtt gaatggacaa aacgaattag tcttttcctt tgtattaccg accatcgatc
    20941 aacgatacca agtggaatcg atgagtctcc taaacgtatc accagagaca atggaaatca
    21001 atgtccaaga tgaccgggtg ctttctttga tccgacactg gtcagtggat cgagtattct
    21061 ctgcagggca gatcacgata ttcaatcaca aattgaatca attattagcc catgcaagaa
    21121 cacttggttt taccgttaat cagacattac tagacaatac gaagccactt cgtttgaatc
    21181 ttcagagcga atttgaattg acgaatgaag tattggatga tatttttatc gtggcaatga
    21241 aaaatccatc ctacgatatg gaaaagatca acgaaactac ttatcaagta cgattagata
    21301 aaggacagtc tttaacgata acaacaaacg aaaaagatca aacagtcttg tcgatctcat
    21361 ctggagacta cccacgatct gtcattgatt tctttatcaa ttacgaattc ttggtcccat
    21421 taatggtgag aaagtcctga ttggataagt tggattacca gatgtcattc agataattct
    21481 gttccaaagg acttgcggca gttatccaga ataattgtca caagtctttt ctgtatgtac
    21541 taaatggacg tgaataactt gctgatgaag gagggatttc attaggaatg tccacaaata
    21601 aaatagcaca gcttagctta ttgtctgctg cttgtgtagc aggtaggatc gcttttcaat
    21661 ttattccgaa tttccagcca atgactgcta tttttttatt tatagtactt tacttaagct
    21721 taaaagatgc gttaattgtc atgagtcttt ctattgcgat cagcagtttt tattttggtg
    21781 ttggaccatg ggtcatcggc caatggatca gctatactgt tgttctgagt ttgttttcaa
    21841 tcgtctgctt gagaaaaaca gtgcaaagga atctatggtt aaagggagtc tgtttttttc
    21901 tggcgggcat ggtttacgga ataggtatga ccgtttttga tacgctgtta tatcgtttgc
    21961 ctcaaccttg gatctattac cttcaagggg tgtcgtttga tttgatgcac agtataggca
    22021 atgttgtgtt ctttcttgtt tttctgccag tcgtcaaacg tttttacaat cattcaggag
    22081 gaaaaaatga aaaaaataat ttatagtttg cttgcaatga gtgtcagtat actcattttg
    22141 acaagttgca gtacaaccta tacacaatcg gcttccaacc aaaaggagga agtaaaaaca
    22201 tcgcaaaaag caacaatcac gttacaagaa aatggtcaaa acttttcgga aaaagaagtg
    22261 gtcttcaaaa aaggcgatga tttactgagt attttgaaac gaaattttga agtcaaagaa
    22321 gataacggct tcatcacttc gcttgaaggg cactcacagg atgagaaaaa tcaactctat
    22381 tggatgttta ctgtggacgg gaaaagtgct actactggtg ccaaagagat caaattgaat
    22441 gatggacaat cggtcatttt caatttgtct aaactataag tttcataaaa aactgttaaa
    22501 gggcgaatat tgccataatt cagtgagttc agttgatgaa agaaggaaaa taacatggaa
    22561 aattttaaaa aaagcggaat gttttccgta attgctgctc taggggctaa tattctagtt
    22621 gctatcagca aatttgtcgg ctatgcaatc agtggcagtg ccgccatgct aaacgaaagc
    22681 atccacagta tcgttgattg cagcaatcag attttgctgt tattcggtga taaacaagcc
    22741 aataaaggac agagcgagtt gcatcaattt ggtgaaggac gagcaaaata ctttttcagt
    22801 acaatcgtcg caatgatgct tttcttcggc ggtggggcac ttggggtgat ggaagctttt
    22861 gaaaagttga cgcatccatc ccatgaagtg ggtaatccga tcattgttat agcaatactg
    22921 ttatttggtt tggtaatcga aggaagttcg ttacgcgtgg caatgaaaga aatcaaagaa
    22981 ttgaatactg aaaaactttc cacgatgcgc tttttgcgcg aaagtcgaca cagtgaaata
    23041 ctgattattt ttacggaaga cttgtgtgct gtaatcggtt tggtcttggc tttagcagga
    23101 acaatattga cttatgcaac cgggattgct gcatttgatg cgatcagcgg attgctgatc
    23161 ggtttcttgc tgatgggagc cgccattttc ctagcaaaag aattttatag tctgatcatc
    23221 ggtgaaagcg tgacagcaag cgatttatca aaaatcaagt tagcttttga acgtcctgag
    23281 atcagtcggc tgatcgatat aaaaacagtt catttaagtc caacggagat tttagttgca
    23341 gcaaaagcag atatcgttgg taacaaagag aatgaagctt actcggtcat caatgatatt
    23401 gaaaaaaata tgcgtcagtc tcttcctgac aagaagatgt atatatacat tgaaacagat
    23461 aaatatgacc caaattattc tcgtaaataa aacgtttatt catgaattga agcaaaaatg
    23521 agaaaaatat aatcttgttt ttaaataaaa cggctacaat ttagcgaatt tatgttacaa
    23581 tgagtccgct gtttatttgt tgtaattaaa ggagatattt taatgaacaa aaagaaaagt
    23641 gcgccatggc ttcaagcttt gcatgcagtg atgccacttt gtataagcta tataccagta
    23701 ggattagctt gcggagtatt gttgcaaaaa gttggatttg atcctctctt atcaggactg
    23761 ttatcgattc tggtcttttc cggtggcgcg cagtttttag ttgcatctat gcttacgacg
    23821 caagcatcat ttgcaacgac attattgatg gttttctttt tagaattacg ctatacatta
    23881 ttaggagcaa gtcttgccgg ttttatgaaa aaagaaaagc ggacgttttt ggctgtcttt
    23941 tctcaatcac tgaatgatga aaactatgcg gtgaactact taaagttttc aactgaccct
    24001 tcctggaata aaagaaaggc tttatatgtc aattggttct caatgactgc atgggccggt
    24061 agcaatatga tcggtaattt tttcggctcg gtgattcgag tagatgctga ccttgtccat
    24121 tttgctttga cggccatgtt cattttcatg tttatcatgc agatgaaaag cgcactactg
    24181 atctttacag gtttattctc aggcgtatta ggggtcgtat tcatggtatt attccaaaat
    24241 actttcggat tgattgcagc aacagtcatt gcttcatttg ccggatttat gctggagaag
    24301 gcattcaaaa aagaaaaagc taagaaacta agagaccgcg gtaaagatgt caatgagccg
    24361 ctcttcaact tcccggaaca ggaggccaat caccatgact aaactatatt tgctactttt
    24421 agttgcttgt ctgttcatcg tggcatatat cccgcgcttg ttcccgatgc tttattttac
    24481 tcatcgaaaa gtgccatcct ggttcagtga ctggatgaaa tatgtaccgg tggcattatt
    24541 tgcggcgtta gcttttaaag atgttttcat tactcatgaa catctggata ttgcttggaa
    24601 tatcaaaata gctgcaatga ttctagtagc aggcgtggct tacaaaacac gttcgatggc
    24661 gttgtcagtt ttgacaggac ttgcgtctgt ctttttgctg tctatgcttt aaactgtttt
    24721 attttattaa aaacagagat tgcgacataa gttgttcaaa ctgatctatc cgaactgcat
    24781 ttgcaacagt caattctgga tgcaagttta ttcgtaggga taaaaaagat tagtgttggc
    24841 ttatgatgca agctcttttt tattttagga attagagagg gatttgtcct tctatgattt
    24901 tttgctataa tagggaagat gtttatttta tgaaagaata gttgagggaa tttaaatgaa
    24961 taaaaaattg atgcaaggaa cattttggct aacctttgct aatcttctat gtaaagtttt
    25021 aggggtagtc tatttgattc cctggctaag tatgatgggg aacaatcaag atggtatgct
    25081 tgctacgaca ttgtataatg ttggctatct tccttatgga ttgttcctga tgcttggtac
    25141 agtcggattt ccaaatgcga ttgcaaaaaa agtagctgta gccacaaaag aaggagataa
    25201 cgctgcttgt cggctgattt ttagaagtac gatcaatatc atgtttgtca tcggtatcgc
    25261 ctctgctgca ctgatgtatt tatttgctcc tttactagcc ggaatcagtc cgattgctaa
    25321 cgttaataat gggatactag ctatcagaag tttatgccca tctttgattg ctatcccaat
    25381 tcttagtgca atgagaggat atttccaagg aaaaaatgat ctgcggcctt atggaacttc
    25441 tttgattatc gaacaggctg tgcgagtgat cgttatcttg gctggtactt attacttgcg
    25501 cgtattgact gacggaacca tattagaagc tgtattgatc agtacagtcg cttctttctt
    25561 cggtgggtta gctgcgatta tccatatgta tatcgtcgga cgcagaaaag acttcttcga
    25621 acttaaagat tttttgattt ccagccgtta tttcgaaaga gaaaaccgat ctgcctctgt
    25681 ctctatcatt cgggaaacac taccgtttat ttttgtcggc tcagtcatta ctatcgttca
    25741 aatgatcgac caagtaacga tgaaaccact gcttcacttt ttccgtccag agatcgcaga
    25801 tcagcaactg gaattcttat tcagccgtgc ttctgtcaat ccaaataaat tgacattgat
    25861 cctgatctcc atggtaggaa cagtagctat aagcagcttg ccaatattaa gcacgatgaa
    25921 gaaaaaagat cggatacaaa tcgaaaaaac agtaggagat agtttttcga ttgcgttatt
    25981 gattcttctt ccttcattga ctggaatgtc tttattagct ggaccactat acacattgtt
    26041 ttttggctat gatcctgaaa gtgtcggata tttccaaatg gctttgctag cttctttgtt
    26101 tttctcttta ttcacgatcc tatctacgat gcttcaatct ttgaatcacc atcgttttgc
    26161 gatccgatta actgtagaag caattatcct gaaagtcatc ttccaagtaa tcggattagg
    26221 attagttggc ggatacggga tgagcttatc aaatggctta gcattcggta ttgtctttgt
    26281 tcgcggctat cactttatgt gcaaagaata ccgaatagca cctttagcaa agatcagcaa
    26341 ttttttctta aaaactttcc gctccaccct gattatgctt gcagtatgtt ttgccgtttt
    26401 ctttttgctg aatcagcgat tgtcaatgga atcaaaaagc tatgcaatga tttattgtgt
    26461 ggctgtagga agtattggcg gcttagtatt tttattttct caattcggaa aaaatgggtt
    26521 tcgaatgttg aaaaatgtta aacatcatag cagaagcaaa gaataggtta actaatcgtt
    26581 ttttgctgtc acttgaaaaa
//
LOCUS       DAZKLA010000027        25610 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000027 DAZKLA010000000
VERSION     DAZKLA010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.027"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..584
                     /locus_tag="ACG85N_000966"
     CDS             30..584
                     /locus_tag="ACG85N_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0223760.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(757..1194)
                     /locus_tag="ACG85N_000967"
     CDS             complement(757..1194)
                     /locus_tag="ACG85N_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0223761.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1242..2189
                     /locus_tag="ACG85N_000968"
                     /pseudo
     CDS             1242..2189
                     /locus_tag="ACG85N_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            2460..2895
                     /locus_tag="ACG85N_000969"
                     /pseudo
     CDS             2460..2895
                     /locus_tag="ACG85N_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            2911..3744
                     /locus_tag="ACG85N_000970"
     CDS             2911..3744
                     /locus_tag="ACG85N_000970"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0223762.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            3989..5014
                     /locus_tag="ACG85N_000971"
     CDS             3989..5014
                     /locus_tag="ACG85N_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0223763.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5152..5277
                     /locus_tag="ACG85N_000972"
     CDS             5152..5277
                     /locus_tag="ACG85N_000972"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223764.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            5302..6417
                     /locus_tag="ACG85N_000973"
                     /pseudo
     CDS             5302..6417
                     /locus_tag="ACG85N_000973"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            6500..7396
                     /locus_tag="ACG85N_000974"
                     /pseudo
     CDS             6500..7396
                     /locus_tag="ACG85N_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            7412..8236
                     /locus_tag="ACG85N_000975"
     CDS             7412..8236
                     /locus_tag="ACG85N_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0223765.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8268..9578
                     /locus_tag="ACG85N_000976"
     CDS             8268..9578
                     /locus_tag="ACG85N_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFQ0223766.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9726..10985
                     /locus_tag="ACG85N_000977"
     CDS             9726..10985
                     /locus_tag="ACG85N_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0223767.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            10995..11867
                     /locus_tag="ACG85N_000978"
     CDS             10995..11867
                     /locus_tag="ACG85N_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0223768.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11879..12709
                     /locus_tag="ACG85N_000979"
     CDS             11879..12709
                     /locus_tag="ACG85N_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0223769.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12749..14932
                     /locus_tag="ACG85N_000980"
     CDS             12749..14932
                     /locus_tag="ACG85N_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0223770.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            14993..16213
                     /locus_tag="ACG85N_000981"
     CDS             14993..16213
                     /locus_tag="ACG85N_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0223771.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16210..17670
                     /gene="gtfA"
                     /locus_tag="ACG85N_000982"
     CDS             16210..17670
                     /gene="gtfA"
                     /locus_tag="ACG85N_000982"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFQ0223772.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17747..18853
                     /locus_tag="ACG85N_000983"
                     /pseudo
     CDS             17747..18853
                     /locus_tag="ACG85N_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19175..19384
                     /locus_tag="ACG85N_000984"
     CDS             19175..19384
                     /locus_tag="ACG85N_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0223773.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19377..19526
                     /locus_tag="ACG85N_000985"
     CDS             19377..19526
                     /locus_tag="ACG85N_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFQ0223774.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19680..20345
                     /locus_tag="ACG85N_000986"
     CDS             19680..20345
                     /locus_tag="ACG85N_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0223775.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20629..21714)
                     /gene="xerS"
                     /locus_tag="ACG85N_000987"
     CDS             complement(20629..21714)
                     /gene="xerS"
                     /locus_tag="ACG85N_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFQ0223776.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22048..22281
                     /locus_tag="ACG85N_000988"
     CDS             22048..22281
                     /locus_tag="ACG85N_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFQ0223777.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22278..22685
                     /locus_tag="ACG85N_000989"
     CDS             22278..22685
                     /locus_tag="ACG85N_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0223778.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(23822..24340)
                     /locus_tag="ACG85N_000990"
     CDS             complement(23822..24340)
                     /locus_tag="ACG85N_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFQ0223779.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            24707..24955
                     /locus_tag="ACG85N_000991"
     CDS             24707..24955
                     /locus_tag="ACG85N_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223780.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
     gene            25458..>25610
                     /locus_tag="ACG85N_000992"
     CDS             25458..>25610
                     /locus_tag="ACG85N_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0223781.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTS"
BASE COUNT         8721 a         3921 c         4789 g         8179 t
ORIGIN      
        1 tcagaatatt tttaagaagg gaaacaatag tgagctataa tgtacaaccg ttaaggacaa
       61 atgaagagat acaagatttt cagttttggc taagaagaac aagtaatccg gaaagagata
      121 gttttttatt tttgtttgga atcaataatg ggttacgtat gtcagatatt ataggattaa
      181 aagttggaga tatacgaggc aaaagtaagc ctattattgt tgaacataaa acaggtaagc
      241 gaaaaccgat ttttatagat aatttgagag aagaaatttt attatatact gaaggtaaag
      301 aagaaaatga ttggttattt ccgagtcggc aacaaggtcg acatataaca agggatcgtg
      361 tctatcagat atatgcagat atagcagaaa aattaggacg agatgatata ggaacacata
      421 cattaagaaa aacttttggg tatcactact acaagaaaac tagagacatt gctaccttaa
      481 tgtttatttt taatcatagc tcgcaagcaa ttacaaaaag gtacattggt ataactgaag
      541 atgaaatagg agcatcactt cgtggattta aactaggtgt ataatttaaa aatttttata
      601 aacagtaaag acaaagatta aaagctattt ttatctagaa cgattttatt tcataaagtt
      661 cgcggaataa aagctggggt tactggtact tggtataggt tcgtgttata taaactaagt
      721 aattaaataa catttttagg ttggaaaagg atgaaattaa aaaaagagaa tcaatacggt
      781 ctcaaaaacg caaacgctgc ggaataagaa aaatactgat aactcaacgt ttagcagact
      841 atctaatagg gatagtctgc ttttttcgtt tttgaccata cttttgacct tctgcaaact
      901 tccttgcttt tcctctgtag aactcatgaa atctgcaaaa cgaattgccg tttcctctgt
      961 ctgctttttc gcgatatggg tgtatatatc atccatagtg gttttaatat ctgtatgtcc
     1021 aagtcgaaca tacacttctt tgacagacgc accagattca aagagcagac tacaatgggt
     1081 atgacgaaaa ccatgaagtg tgatatgttt taagttattc tctttaatga tgattttcaa
     1141 attgtgattt acatagtcta aataaagggg cttattttct tctgatgaaa acaaaagttg
     1201 ttcaccatct tttataggtt tgattctcat ttttagatat tatgcccgca cgtattcatc
     1261 acatttctat tgtgaatcgc tttattcgac cgacatttga cttttatcat aatatactcg
     1321 gtttaaaatt actaatgaaa acaattaatc aagacgatca taccatgtat cacctgtttt
     1381 tttcagataa tcaccaccgt gtgggaactg aactgacttt ttttgaagta caagaaggga
     1441 acaaccagtt ttttggaacc aatacgattg aacgaacgat actaaaagtt ccttccgaag
     1501 catcattgca tttttgggaa atatattttg aaacacaagg cgtttgccat tatggaatcg
     1561 aatcattttc tggacgacct atcttacgtt ttgaagggcc agatgcaaca cgattagctt
     1621 tagttccctt acgcgaattt gaagatattg atgattattt cccgtatgtc acagaccaga
     1681 tccctacaga gcatgccatt ttaggaattg atgccattca actacgcgta caatattccg
     1741 atgcgactga acgagagtta ttgtcggtag gctggtagca caaagaatac gtcagtttct
     1801 tttcgggtca tcaacgagtg acaatcttag aaaatcaaga cactcagttc tatcaagaag
     1861 ttcagattat tcaagatcgt gagaatccag ttgccattga agggattggt agcgtccatc
     1921 atgttgcgtt tggcgtagaa aataaatctg atttacaacg catcgacaaa caattacaag
     1981 agcgcaattt tataaattca ggaataaaag atcgtgaatt ttttgtatca ctttattatc
     2041 gtgacccaaa tcaattattg attgaaattg ctacagggga aggaaaccta gatgcaaaag
     2101 cgtatgaaaa tcaaagtcca cgctttgaag aaattccttt atttttaccg cagtacttaa
     2161 atttcattcg tgaacaagtg gagcaataat tagctaatca aatccatgac gaagagtaag
     2221 aggttgactc ataagtcaaa aaataaagaa aaacgcatca aatccattct aaagaatgtt
     2281 gatttgatgc ggttcctatt tttataaact atcattttcg actaataaaa ctacttatga
     2341 gtcagcctct ctttttttct tgatttttat acctctttct aggtaaacta aacatctgcc
     2401 aaaaataccg tgtctcattg aaacattctc aactatttca aaataaaggg gagtaccaaa
     2461 tgaacgagta tattgaaatt atccacgcaa gagaaacaac ttaaaaatgt cagtctcaaa
     2521 attcccaaaa ataaaattag tatttttacc agcgtatctg gttcaggaaa atcatcgatt
     2581 gtttttgata ctattaccca agaagctggt cgtcaactga atgacaccta tgatagtttt
     2641 acccgtttat ttttacctaa atacagtcgt ccagaggttg atgagattga taatctttct
     2701 acggcgattg tggtcaatca aaagaagatt ggtggcagtg cgcgctcaac gttagggacg
     2761 attactaaca tcaatgcgtt atttcgtgtg ttgttttcat gttttgcaac acctagttta
     2821 ggatatgcaa atgcttattc atttaatgat cctagtggaa tgtgttctgt ctgtcaggga
     2881 atcggtaaga cgattgcatt tttaatagga ttgctttatt tttcggctat tgataatgct
     2941 gattttgtat tttctgatcc atttaccgca gaagttttgt ctggaattga ggaacaggca
     3001 aagaatcatg gttattttgt attggtgcat aatgttaatt cagcagaaga tgttaaaact
     3061 attcagagaa attggcgttt tgaggggttc attgttgttg gtgtacgtgt aaaagaattt
     3121 ttggaattaa ataacgtttt tgatggtcct gttgtttata ttgatacgca tctttcaaaa
     3181 gagacattaa gagaacttga acaacaaatg aatcgtttat ttgtaaatac aaatgattac
     3241 caagctagta cattggcagt ggaacatttg attcattatg gtcatgagaa aatagcgttt
     3301 ctttcttttg actacattgt aggtgaacca agtgttattc aagaacgaca tcaagcttat
     3361 atcgatacat taaagaaata tcatttaacc tttaaatcgc attggatata taacaactct
     3421 gaatttgagc gaatttatca agaattagct gaatttactg caattgttgt tactggagat
     3481 tatttagcgg ctaaattagt aaaattttta aaaaataaga ataaacgagt gatggaacaa
     3541 ttatcgatta ttggatttga tgatattact tttgcagaat taatggatcc tgcgttgaca
     3601 acaatacgac taaatccagg taacaagggg gcgattgctt ttcaccaact gattggactg
     3661 gccaaacaag aagaaataaa aaatcaagta ctactattag agggagaatt gatttctcga
     3721 gactctgttc acaaaatcaa ataaaaaagc tctgtgaaat aagtatagtt aacaataaaa
     3781 tatccgaacg atagagagaa tgaactgtga tctagcagtt cctcgtcgca aattcactag
     3841 aattagcatt atttactcta aattgttcgg atattttttc ttgtttttaa ttatgattac
     3901 agtgcctatt tttaacgaaa tttgcttatt ttcaaaatgt atgattatat gttagtaaac
     3961 actttcaaat atgaaaagag gagctattat ggtaggaatt agggatgttg caaaacgggc
     4021 gggtgtgtcc attgcaacag tatcgcgcgt gctaaatgag gatccgacac tttcagtaac
     4081 cgatgaaacc aaacgaaaga ttgcagaatc ggttaatttt tatcattatg tcaaaaaaag
     4141 tcccgttgca aaaaatttaa caaatattgc attaataacg acagtttctg aagtagatga
     4201 gttggaagat ccctattttc gaacgattcg cagagggatt caagtcgaag cagaacaaag
     4261 caaagtcaat ttgaagaaaa ttattcgttt gtcagaaaca ccgttggagc aagacgattt
     4321 gctaaaatgt caaggtgtgt tgattattgg gcaggtgcta tcttctgtga ttgaagaagt
     4381 atcagcaatt aattcgaatg tcgtagtgat tgatgaccct agtattgaag ctgaagtaga
     4441 tgcagtttat actgatttaa caaaagcaac gaatcagcat ttagatcgat tgtataaaaa
     4501 aggacaccga aacattgcgt ttatcggagg aggacgtgtt cgtttagata gccaaggaaa
     4561 taaacacgtg tcagatgatg attatcgaaa aatagcctat gagacgtgga tgaaacaaaa
     4621 aggattagaa aaatatagtc aaatttattt agaaggctgg accacacttg acggaatgca
     4681 ggcagcagat ttacttttga aaaaacatgc taaagataag ttgccgactg ctatcatggt
     4741 aggaaatgat cctattgctg tgggggttta tcgttcactt caaaaaaatg gtctacatat
     4801 tccagaagat atttcgattg tgagttttga taatattgaa gttgctgaat ttttaacacc
     4861 tgcattaaat acggtgaatg tgaatacaga agaaattgga aaactagcag ttcgcatggt
     4921 taatgaacga ataaaagaag ttagaaatgt ttcgatcaaa gtgattgttt ctaataatat
     4981 tattgttaga gaaagtgaaa aaaatatcga ttaaaaaagt aaacatccaa aaaagggtgt
     5041 ttactttttt gtttactaat atttttagta aaaaatctcg acaaaacatt aagtaaacag
     5101 ttatggtgtt gttaacaaaa agaaaacgca ttcaaaacag gaggaattaa gatgaaacat
     5161 ttcaaaaaaa tagctttaac agggatagct gttttaactg ggacattact cgcagcatgt
     5221 ggtgggggag atagtaattc taaagacggg caagtaactt taagctatgc gagttgagat
     5281 actgaccaag ctccaggact aagaaaaatg ttagatgaat ttgaaaaaca aaatcctgat
     5341 atcaaagtag aaatggagac aacgccttgg gatcaatatt gggttaaatt agaagctgca
     5401 acaacgggag gtaatatgcc agatgtagta accatgcact ctagcgaatc ctataaatat
     5461 atgtcacaga atgctttgat gggacttgat gatgttatca aagaaaatga tattgacatg
     5521 ggaaacttta ctgaaggaat tgctgatttc tatacgcttg aagacgaact atacgcaatc
     5581 cctaaagatg cttctgtagt ggggctatgg tacaacaaag aattatttga taatgcaggt
     5641 gtcgattatc cagatgaaac atggacatgg gaaacactca gacaagctgc aattgattta
     5701 acagatgaat caaaaggcat ctatggtttt gcatcacata ttggaacaga gactggctac
     5761 tggccattta tttatcaaaa tgggggagaa gtatttgccg atgacaatac aaaatcaggt
     5821 ttgaataccc cagaagcaca agaagcttta gctttttatc gagatattgc acttgttgat
     5881 catgcttcac caacaattac agaattacaa gatcctgata agttaagtcg ttttcaatca
     5941 ggtagattag caatgatgat tgaaggaaac tggtttgcac cgggattttt gaaaaatgac
     6001 tatctagttg aaaatgttgc ttctgccgtg ttacctaagg gaaaagttaa tgcaacaatt
     6061 acaaatggac ttggttgggc tgccagtgcg agtacaaaat acccagaaga agtgaaaaaa
     6121 ctaatggcat ttttagctag tgaggacgcc aataaaatcc aagcggaaac gggggcatca
     6181 atcccagcac taaaaggctt tgaagacgag tgggctgctt cttcagatac aattgatttg
     6241 agtgcttttg gaaaaatgtt ggagtatgga gtccctcgtc catacaataa agatggatta
     6301 cgagctgaac aagcagaagt tcaaattatt aaccgtattc tgtctggtga agtaactgtt
     6361 gaagatggaa ccgctcaaat tgaaaaagaa gtcaatgcga tattgagcgg aaattaaatt
     6421 agttaattat acaatataca ggcgaaagga ttctatttcg tctgtatatt aatggatgaa
     6481 taccaaaaag ggggatatta tgaaaaatcg aatttttgga aaatatcgta aagatcaagt
     6541 gatatggggc tatattttta tttttccaac ggcattagga ttaattttat taaatatatg
     6601 gccagcaatt caaacagcaa ttttaagttt tcaaaaaacg gtagggttcg gtgatactga
     6661 atgggtcgga ttaagaaatt accaaaaaat gtttgcagac ggtgaagttt ttcagtcttt
     6721 attaaacaca ttaatttatg cagtggtttc gatacctagt attgttgtat tatccttgtt
     6781 ggtggcagtt ttaatgaata gaaaagttaa aggattgtcg atttatcgga caatttattt
     6841 tctaccagtc gttgctgcac catccgcagt cgcaatgatt tggcgctgga tgttcaataa
     6901 tgattatgga ctgattaata acatgttggc taaaataggt ctcgatggac cagcatggtt
     6961 aacggatcca tcgattgcga tttattctat tatcattgta ggagtttgga gtgctattgg
     7021 ttataacatg gtgttattac tagccggctt ataagaaatt cctaaagatt attatgaagc
     7081 agcggaaatt gatggtgcta gtccaatcca gcagtttttc aacattacgt taccattagt
     7141 tacaccaaca ttattttttg tagttgtaac aacagttatc aatgctttcc aagtatttga
     7201 tgttattttt atgatgatta ctcctaatag taccgccatg tttaaatcac aatcattagc
     7261 ctatttattc tataaacatt catttatttt aaatgacaag ggatatggtt cggcaatcgt
     7321 tatgttcctt ttagtgatta tattaattat tactgcaatt caaatgaagt tgcagaaaaa
     7381 gtgggtaaac tattaaggag gctatcaaat tatgaaaaat aaattttttt caaatgcgcc
     7441 aatccatatc atgcttattt taggtggtat tgcaatggca ttacctttta tttggatgat
     7501 tttaacttca gggaaaacgt tggttgaatc tacccaaatt ccacctacaa ttttccctga
     7561 gaaatttcag ctaagtaact atcaacaagt acttgattta ttaccattca tggcttttta
     7621 tatcaataca tttttaatgg ttgttggacg agtgattggc tcggttttat tttcagcaat
     7681 ggctgcatat gcttgtgcgc gtctaagttt tccagggaaa aatatccttt tcagcttagt
     7741 acttattcaa atgatgattc ccggttccat ttttattact ccacagtatt tattagcaca
     7801 aaaattgggt attttaaata caattccagc attaatatta ccaggggtag ttagtgcgtt
     7861 tggtacgttt ttattaagac aatttttctt aggtttacct gatgaattgg aggaagcggc
     7921 ctatttagaa ggtgccaata tttggacgat ctttttcaaa attatgttgc cgcttgctaa
     7981 atcaggattg gttgcacttg gaatattcac agcgttgttt gcatttaaag atttaatgtg
     8041 gccattgatt gtcaatactt caattgataa gatgccttta gctccgggat tagcaaactt
     8101 acaaggacaa tatgccacga acttcccaca attaatggca ggttcgttat tagcaatttg
     8161 gccaatagtt gtgatattca tcgttttcca aagaaaattc attgaaggaa ttgctacaac
     8221 aggtagtaaa ttataaaact attaattagt gattggagag taagtacatg acaaaaatta
     8281 catttattgg tgcaggaagt actatttttg caaaaaacgt attaggcgat gcgatgttaa
     8341 ctccaagtct acaagatgca catattgcat tgtacgatat tgatgaaaaa cgactaaaag
     8401 aatcaaattt aatgcttaaa aatattaata aaaattcgaa tgaaaatcgt gctgatattg
     8461 tttcttatta tgatcgcaaa gaagcgttaa gagaggctga ttttgttatc aatgctattc
     8521 aagtgggtgg ttataaacct tcaacagtga ttgattttga tattcctaaa aaatatgggt
     8581 tgcgacaaac tattggtgac accatgggga ttggtgggat ttttagaacc ctacggacac
     8641 taccagtaat gttagacttt gctaaagaaa tggaagaagt ttgtccaaat gcttggttat
     8701 tgaactatac aaatccgatg gcgatgttaa ccatgggtgt tttaaaagcg acgagtatta
     8761 agactgttgg tttatgtcat agcgttcaag tatgcgtacc agaattgttt gagcatttag
     8821 gaatagccga tcaatatgac ttaaatgact ttcaatggaa aattgcaggc attaatcata
     8881 tggcattctt gttggaaatt acaaaaaatg gggaagattt ctatccaaca attcgtcagt
     8941 tagctgcaga aaaaccaaat ccacatccag attctgtacg ttttgaattg ttgaaacgtt
     9001 ttggttacta tattacagaa tcaagtgaac acaatgcaga ataccatcca tactttatta
     9061 aatcaaaata cccagaattg attgacgaat tagggattcc gttggatgaa tacttacgtc
     9121 gttgtgaaca acaaattaat gattgggaaa gcatgcgaga ggacattgta aataatgggg
     9181 agttaacaca tacgagaagc cgtgaatatg cttcatatat catggattct attacgacag
     9241 gaactccgtc agtgattgca ggaaatgtct taaataaagg cttaatcaca aacttaccga
     9301 cagatgtttg tgtggaagta ccttgtttag tcgataaaaa tggcgtgcaa ccaacctatg
     9361 tcggtgaatt gccaacacaa ttagctgctt taaatcggac gaatatcaat gttcaagaac
     9421 tgacagtaga agccgcttta acactagata aatcaaaaat ttatcaagca gcgtatttag
     9481 atccacatct atcatctgaa ttgtctttaa cagatatcaa aaatttagtg gatgatttaa
     9541 tcgaagcaca tggggactat ttacctgaat ataattaaaa agaagctgaa agagtaagga
     9601 attttcaatt ccttactctt ttcttagtta aatttagtaa aaatattgac aaaacactta
     9661 gtaaacagtt acattagtgt taacaaaaag aaaacgcatt caaattgtat aaggaggagc
     9721 ataaaatgaa gtggtctaaa aaaattattt tagcttgtat agcactagga gcaacgatct
     9781 ctttgactgc ttgtggaaaa aatcaggaag atgcaagcgg taaggtagaa atagagtttt
     9841 ttaatcaaaa aaaagaaatg actcaaacga ttcaggagat tgctaaagat tttgaagcaa
     9901 aaaaccctga tattcatgta aaagttgtgg atgtaccgaa tgctggtgaa gtaattaaaa
     9961 cacgtatgct tgcaggcgat gtaccagatg tcattaatct ttatcctcaa agtatagaat
    10021 tgaaagaatg ggcgaaagct ggttatttag aggacttaac cgaagaaccg tatttagaga
    10081 atatcaaaaa tggatatgca caacgttttg ctattgagga tcgtgtatat agcataccat
    10141 taaccgctaa tgtctatggt ttttactata acaaaactgc ttttgaggaa atggggattc
    10201 aagcaccaga aacttgggca gaatttgaaa aaatagtagc tgagattaaa gatcaaaaca
    10261 aagtaccgtt tgcgattgca ggagcagaag gctggacttt aaatggttat caccaactag
    10321 ctatagcgac tgctgctggt ggtgaagagg aagcgaacaa tgtttggcgt ttctcagaag
    10381 ttaatggaat taatgctgaa agcaaagaaa tgcaaaaaga ttttaatcga ttagatttgt
    10441 taagagaacc aaaagctttg caaaacaact ggcaaggtgc cggttataac gatacagtag
    10501 ttacttttac aaaaggtgat gcgttgatta tgccgaatgg ttcatgggca atgccgatga
    10561 ttcattcaca aaatccagat tttgaagtag gaacttttcc ttttccagca gatgaagcag
    10621 gtcaaagtct aacgattggc gcaggagacc tagccttatc agtttctgca acttctcaac
    10681 ataaagaaga agccaaacgt tttgtagaat atatgacaac acctgaagcg atgcagaaat
    10741 attatgatgt agatggtgca ccgtgtgctg ttgagggcgt tatcgagaat actcaggatt
    10801 caccattaag tggtttaact gaacttgctt ttactgatcg tcatttagtt tggttagcca
    10861 aagattggaa tagtgaaaat gatttttata ccttaacaac aaattattta cacaatggca
    10921 atcaaaaagc catgattaac gcattaaatg ctttctttaa tccaatgaaa gctgacggaa
    10981 ggtaggaaaa taaaatgaaa ataaaagaaa ttttaaatcg ttactgggca atcatttttg
    11041 tagcaattcc aattattttg cagatgattt ttttctactt tccaatggtt caaggtgctt
    11101 tttacagttt aactaattgg actggtctga cttataattt tgattttgtg ggtttgaaca
    11161 attataagat tcttttttct gatccgaagt ttatcaaatc gattggcttc actttaattg
    11221 tgacgctttg catgattgct ggacaaattg gttttggtat tttgattgcg cgggcgttga
    11281 acgcaaatat taaagggaaa acattcttta gagcatggtt cttttttcca gcggtcttat
    11341 cgggcgtggt tgtttcttta gtatttaaac aaatttttaa ctatggttta ccaaaaattg
    11401 gtagtatgtt aggcattgaa tttttacaaa ccagtctttt aggaacaaca tggggagcga
    11461 tttttgcagt aatttttgtt ttactttggc aaggaattgc catgccagtc atcattttct
    11521 tagcgggttt tcaaagtatt ccacaagaaa ttattgaagc tgctagaatg gatggggcga
    11581 atgataagca aattttttgg aatattgaaa tcacctactt attaccaagt atttcaatgg
    11641 tatttattat ggcattaaaa ggtggcttaa cagcatttga tcaaattttt gctttaactg
    11701 gtggtggacc aagcgatgcc acaacatctt tggggttact tgtttataat tatgctttta
    11761 aaaacaatca gtttggttat gcgaatgcta ttgctttagt cttatttatc ttaattggga
    11821 ttgtttcact ggcacaatta aaattatcga aaaaatttga aatctaggag gtttcccaat
    11881 gaaaacatca aaagaaaaaa atatttctaa atacttgctt ttattattag ggggcttcat
    11941 gattttagtt cccttagttt caacgctatt cggttcattt aaaacaacac ccgatattat
    12001 gaataatttc tttcgttttc ctaatccagc gactttggaa aatttccgaa gattgttagc
    12061 ggatggaatt ggtcattact tttggaattc ggcaatcatc acaatcattg cagtagttgc
    12121 tgtgacagca attattccta tggctgctta ttcactagca agaaaaatga ctcgtgggaa
    12181 agcatacgga ttgatgtatt tatttttaat tttaggaatt tttgttcctt tccaagtaat
    12241 tatgattccg attaccgtta tgatgagtaa aattggttta gcaaatatgt ggggattaat
    12301 cttactctat ctcacttatg ccgtaccaca gacactattt ttatatgttg gttttattaa
    12361 actaaatgtg cctatcagtc tggatgaggc tgctgaaatt gatggggcta gtcactttac
    12421 aacttatttc aaaattgttt tcccaatgtt aaaaccaatg catgcgacga ctattattct
    12481 taatgctcta tggttttgga atgatttcat gctaccatta ttaattttaa atcgtgattc
    12541 taatatgtgg acactaccat tgtttcaata taattatacg ggacaatatt ttaacgacta
    12601 tggtccaagt tttgcttcct atacagtagg aattgtcacg attacactag tttatcttgc
    12661 tttccaaaga agtattatag ctggaatgag caatggagca gtgaaataac caataaatta
    12721 ttctcattat aaaggagttc ataaacttat gattatattt gacaatcaca cacaaacatt
    12781 tcatttacaa aataaaaaaa ttagctatct attaggaatc gaagaaaata attatttaac
    12841 gcatctttat tttggaaaaa agattacgca ctattctggt gggtatcgct acccccgaac
    12901 agatcgttcc ttctcaccca acaatgcaaa tgcgcaagat cgtttattat ctttagacac
    12961 attaccatta gaatttccag gatatggaca tggtgatttt agagaaccgg cgcataatat
    13021 taaattagga aacggtagtc gtgtaaatga ttttcgttat gatagctacg aaattcaaaa
    13081 aggaaagccc agcttagcgg gattgccagc cttatatacg gaagatgatg atgaagctga
    13141 aacgctgatt attcaattga aagacgcgat tagtggattg actttacagt tgttctacac
    13201 aatttatcaa aatcacagtg taattattcg ttctacgcaa ttagtaaata atagtgatga
    13261 aacagtggag attaaccgct tagctagtca atcaattgat tttccaaatc gtccactcga
    13321 gttaattcat ttaaatggta cctgggcaag agaacgtcaa atgactagag aaaaaattca
    13381 tacagggata aaagtattag atagtaaacg cggatcaagc agccatcaac aaaatccgtt
    13441 tgttgcttta gtagatgcca aaacagatga atttcaagga gaggcatatg gatttaattt
    13501 agtttatagc ggtaatcacg aaacggtgat tcaaaaagat ccatttgacc aaacacgggt
    13561 cattatggga attaattcat ttaattttgg ttggcaacta aaaactggcg actcatttca
    13621 aacgccagaa gtcgtgatgg tttatagtaa tgaaggtctg aatacgttat cacagacgta
    13681 tcatgaattg tataatcacc gtttaattcg tggaaactat cgtttaaaag aacgtcccac
    13741 gttaatcaac aactgggaag caacgtattt tgattttaac gaagaaaaaa tcatgaatat
    13801 tgtcgatgat gcgaagaact tgggcattga aatgtttgtt ttagatgatg gttggtttgg
    13861 cggtcgtgaa aatgacttca cttcattagg tgattgggtt gaaacagaag gcaaacttgc
    13921 ttgtggtttg gaaaatttag ccaagcaagt gcatgataac gggatgaaat ttgggatttg
    13981 gtttgaaccg gaaatgattt ctgaagatag tgaactattt cgagcacatc cagattgggc
    14041 attgggtatt ccaggtcgtg accgctcaat tagtcgaaat caatatgttc ttgatttttc
    14101 aagacaagat gttcgtgata atatctatca acaaatgacg gcgatttttg atcgtgtacc
    14161 gattgattat gtgaagtggg atatgaatcg aaatatgaca gaagtctatt ctgtcttgtt
    14221 ggactcaacg atgcaaggtg aagtggctca tcgttatatg ttagggttat atgaattttt
    14281 agaaaagtta acaacgaatt atccaactat tttatttgaa agttgttcgg gtggaggcgg
    14341 tcgttttgat gcaggtttct tgtattacat gccacaaact tggacgagcg acaataccga
    14401 tgccgtggct cgtctgaaaa ttcaatatgg aaccagctta gtgtatccaa tttcaagtat
    14461 gggtgcgcat gtttcagcag tcccaaatca tcaaacagga agagaaacga gtttaactat
    14521 tcgtggcaat gctgcgatgg ctggtgtttt tggttatgag ctagatctaa cgcaattaac
    14581 ttctgaagaa aaagcagaaa ttaaagaaca agttgccttt tataaaaagc atcgccaact
    14641 tttgcaatat ggaacttttt atcgcttgga aagtccgttt gaaggcaatg atactgcatg
    14701 gatgtttgtc tcaaaagacc aaacagaagc catggtcttt tatttccgag tattagcaga
    14761 agctgcgcat ccattagtca cattgaaatt agcaggatta gcagaagaag caacttatca
    14821 aatggaagat gtaacatttg ctggcgatga attgatgaat ctcggttttt atattgactc
    14881 agaactacat ggtgattatg cgacacaacg cttttatttg aaacagatat agtagcttga
    14941 atttacaata taagtgcaac accaaaagag actcattcca aacgaagaaa atatgtttta
    15001 tttacacgca tttactaaaa agcagcctga tttgaattgg gaaaatgaag aagtacgtga
    15061 agaaatctat gcaatgatta actattggtt agataaagga ctaggcggat ttagaatcga
    15121 tgcgatatta aacatcaaaa aaaaactaga gtatggcatc tttgaatctg atggagaaga
    15181 tgggttagcc ttcattgggc cctggattct taatcagtcg ggcattgaag tgtggttaaa
    15241 agaaatgaga gagcggacat ttaaaccaca taatagtatg acggtagcag aagccgatgt
    15301 tcctaatgaa cgtttagatg aatatattgg tgaagatggc tattattcga tggtttttga
    15361 ttttagttat acggatattg atgtacctga aactggtgaa tggtttaaag atagccaatg
    15421 gacatggacg gatatgcgta ccaatatttt tacgaatcaa ttagttacgc aagataaagg
    15481 ttggggcgcc ttatatttag aaaaccacga tcaaccacgt tcaattaata aatatattcc
    15541 agaagaatgg atcaatgatt atagtaaaaa aatgttggcg acgcttttta tgttactaag
    15601 aggtacccct tttatttatc aaggacaaga actaggaatg accaacatag aaatggcatc
    15661 attagaagat tttgatgatg tagctacgca ttctcagtat aaacgggcat tagaatatgg
    15721 attggcacca gagcaagcat tgaaagctgt caataaacga agtcgggata attctcgcac
    15781 acctatgcaa tggaatacgc aaaaaaatgc cagattctca acttctgata atgtatggtt
    15841 aaaagtaaat cctaattatc cagaactcaa tgcagaagct cagttaacca atcaacagtc
    15901 ggtattcaat tattatcatc aattaattaa attaagaaaa aatagtgtat acaaagacgt
    15961 gctaatctac ggacaatttc taccagtgaa agataaaaat gaacatattt tgctttatga
    16021 acgccaatta gaagataaaa tcgtattagt tatcttaaac gttacagcaa cagaacaaga
    16081 atattatgta gatgaacagt accaacaagt attaatcaat aattatgaag atgttttatt
    16141 agatcaaagt cgaaaattgc gtttacgacc gtttgaaagt attgtattag ctaattatgg
    16201 aggacaatta tgaaaattaa aaacgaagca atgctcatca cctatgcaga tagtttagga
    16261 aaaaacatgg gcgaattaaa acaagtctta gacaaacatt tacaaggtgt tattggtggt
    16321 gtacatctgt tgcctttttt tccatcgaca ggtgatcgag gctttgcgcc gagtgattat
    16381 acgacagttg atccagctct tggaacatgg gaagatgtgg aagcattagg tgaagattat
    16441 tatttgatgt ttgattttat gattaatcac atttctcgtg aatcaaaatt tttccaagat
    16501 ttcaaaaaaa accatgagca gtcaccatac aaagaaatgt ttatccgtat tcatgaattt
    16561 ttcccagaaa atcgaccaac acaagaagat attgatttaa tctacaagcg taaagacaaa
    16621 gcgccgtttc aaacggtaga atttgcggat ggtacgacag aagaggtttg gaataccttt
    16681 ggggaagaac aaattgattt agatgtgacc aaagaagttg tgaaagaatt tattcgagaa
    16741 accatcaaag atatggcgaa ccatggctgt tctttaattc gattagatgc gtttgcttat
    16801 gcgattaaaa aattagatac caatgatttc ttcgtggaac cagatatttg ggaactttta
    16861 gacgaagtgc gtcaagaagc agcgaaatat gacgtcgaat tattaccaga aatccatgag
    16921 cattattcca ttcaaatgaa aattgccaat catgattatt atatctatga cttcgccttg
    16981 ccaatggtca ctctgtattc actttatagt ggaaaatcag aacgtttagc caattggttg
    17041 aaaatgagtc cgatgaagca gtttacaaca ttggatacgc atgatggaat tggtgttgtg
    17101 gacgctcgtg atttattaac agatgaagaa ttggattaca cctctgagga attgtataaa
    17161 gtcggggcga atgtgaagaa ggtctattca agtgccaatt acaataattt agatatttat
    17221 caaattaaca gcacctatta tagtgcatta ggcgataatg accgtagcta tctgttagca
    17281 cgtgcgattc aagtatttgc gccagggatt ccacaaattt attacgttgg gttattagca
    17341 ggaaaaaatg atattgaact gttggaagca acgaaagaag ggcgtaatat taatcgtcat
    17401 tactatgatt tagccgaaat tgaagaagaa gtgcaacggc cagtgattca acaattattt
    17461 gcacttttaa aatttagaaa tgaatctgcg gcgtttgatt tggatggaac gattgaagta
    17521 gcaacgccat cagaatcaga aatagtgatt acacgtaaaa ataaaaacgg tagtgatagt
    17581 gcaacattaa cggctaactt ggatacgaaa gaatttatca ttgagtctaa agatataaaa
    17641 attttcacta gtgttgcatt aaaattttaa cctattccat agaacaaaaa atcctttata
    17701 ctagcacttg gatgacttat ccaagaccaa taaaaaggac tcatacatgg ataagtataa
    17761 aacaaataca gcatttaata aatggttttc tgccataaaa ttaggaaaac tttctgtcaa
    17821 ctcccaaaaa tgtatttttg attacaattc gtatcgtaaa aaactgtctt ttgaagctgt
    17881 gctcaagctc tttctctacg cagttaatga agaaaaagaa agtttgcgtg atttgagtac
    17941 agctcttatc gaccgatcgc tccaactaga aaccgatgtg tcatcgatta gtcataccca
    18001 attgtctcga gcatttaatg cacttgaacc taaagtgtta gaagaaattt ttcaacaact
    18061 gttagaaaaa gttcaacatc aaatcaaacc aaccaagcga aatcatctgt atctgattga
    18121 ttccagtacg ttttcgctta gtttaaaacg tcataagtgg gcgactttcc gtcaaacaaa
    18181 atcaggtgtc aagttacact taaactattg ttatatggac gatactacga tgtatccaac
    18241 tgattttacc ttaacaaatg cccatgaaca tgatctcaat caactaccag tattggtgaa
    18301 tcaaccagag gcaacttacg tgtttgatcg tgggtattag gactttgaaa agatggatca
    18361 gatgcattgg gatggctatt tcttcgtcac tcggattaaa aaaaacacca aagttcacgt
    18421 gatagatact ctggaaacat cgccagaaac agaaattcta cgagacgagc tggttcgttt
    18481 aggatctaaa acctatctaa ccgccaattt ccgactagtc actgttcaag ataagaacgg
    18541 acgtgtgttt caatttatca ccaatcgaat ggacgtctct tcaaaagaaa tctctgatat
    18601 gtatcacgct cgttggcaaa tcgaattgtt tttcaagcac atcaaacaac acatgaccat
    18661 taaaaccttc ttttctcaaa gtgaaaaagg cgtgcagaat caattgattt taacgatgat
    18721 ttccgctctc ctcacgtttc tcatcaaatt ggagacgaaa acggaaaaat cagtgttcca
    18781 aatcaaacga ttctttcgtt atttactgtt tcagcccttt gaatgttgtc tagaaaaatt
    18841 aattcccaca tgagaaaaaa gaaatttggc tcgttacttt tggttttcag ttgaataaca
    18901 gataaaaaat tttctggaaa aagtcatcct ctgcgtcagc acagtttttt ttgacttatt
    18961 ttcagaggaa atagttttca gaatattcta aaatgtttta tgcaacacta gtgatatttt
    19021 ttagaattag atttctaatg tgcatttttt gagtattaag gagcaagtat acaaagattt
    19081 ataaagctga ttaaatttga ataaaaatat gcaggaacgc tactattttt agtagtgttc
    19141 ttttttatat ttgaataaaa aatgtggtgg ttaaatggtg gttataacaa aaaatgaatt
    19201 aatgaaatta ggttatggga agtatcagtc tgagagaatc atacgacagg caaaagcaat
    19261 tatggtttca aaaagctata cctattacga aaatagaaga ttaggactag tacctgttga
    19321 atctgtagaa gaagttttag gtatgaagat agaggtagag gaggattttc agaatcatgg
    19381 ttaattttaa aaaagataaa aatgggaaat atcattttac tgctagtttg ggattcgaca
    19441 aacgaacagg gaaaagagta caaaaaaacg tagaggggtt aaaacagtta aagaagaaaa
    19501 aaagcgtata ccgaacttgt ttctaattat ggaaaagaag cagaaacttc tcaaagtact
    19561 atgttgtttg aggagttttt ttataaatta ttcctacctt attatcaagg gaaagtagaa
    19621 gaaagaacat ataaaaatcg gttatctaca atcaaaaaaa cttttcttat ttctttaaaa
    19681 tggagttaag agaaatacgt ccaattgaca ttcaaaagtg gcaaaataat ttattgagca
    19741 aatataaaaa ttcttatgta agaaacatgt atgggatttt tcagatgtca ttagatcgag
    19801 cagttgtttt gggactactt gaaaaaaatc ctgcaaaagt tattggaaat gtgaagaaaa
    19861 ggaaaactac agttgatttt tggacaaaag aagaatttac aaaagttatt tcaacttttt
    19921 atattgatga tttgtaccaa cattttggtt ttatttgtat gtggttactt tttatgaccg
    19981 ttatgcgttt gggagaagcc actgcattaa cttggaaaga tattgatttt gaaaataaaa
    20041 acttgcgtgt gaataaaaca ctctattata agagagccaa aacctatgag tttgtagaac
    20101 ccgaaacggc ttctagtatt cgtacaatcg aactagacga caatacaatt acctttttaa
    20161 aagcttggaa ggagcgtcaa gtttcaatag gaggcatgtc gtttgtactt tcttataatg
    20221 gaacacctac tcagaaatat acaatccgtc atattatgag aagacatgca acgatcgttg
    20281 gtgttcatat aatccgcata cacggattaa ggcatagtca tgcttctttg cttatatcac
    20341 aataaaaaga tgcacttcga attaaagaaa ggttaaaaca tgctgatgta caaacgactt
    20401 taggaactta tggtcatttg tatctaaatt cattgagaga gataacaaat gatcccacag
    20461 attatatcca aactaatttt tctaaaatca aaccgacacc tatttacgat aataaaaaat
    20521 aaatgataaa caaaagtagg atcacaccaa aagaaacaca ctagaaaaac tataatacag
    20581 gcttttctag cgtgttgttt cttatttccg cctatttata cattatactc ataaagcatt
    20641 taatgcatcg atcgtagccg cattgtctat atgggtatat aaagttgttg tattggtact
    20701 tgaatgtccc aattgttggg cagttaatac caatgaattt gtctgttggt agagtctagt
    20761 agctaatgta tgacgcagtt tgtgagggct gacttgaatt ctaaagcctt ttgaatattt
    20821 tgctaccatc cgttcgattg caataccgga tatacgcttg gcttctcctt ggtaaattgc
    20881 tagaaataaa gctttttcgt tttctgttac tttatatcgt tctttccgaa tagctagata
    20941 attagctaaa tattccattg cgaatgctgc tatattgaca gcatctcttt tatttccttt
    21001 tctagttact accacattat tcttatctaa agataggtca tctaaattaa tatttataac
    21061 ctcggagagt ctcaatccag atcctaaaat cagtgcattg atcgccaggt ctctttctac
    21121 atctcgttga taatagatca aagctcgttt actcaaagta tttttatatt cattggcaat
    21181 ataatctaga taattttgtg tatccttatc tagaaataat tttttctcaa tggcattggc
    21241 acgtgcagct aacgtagttt tatctgtgat ggttgctact tgatccatag gattattgtc
    21301 taagtatggt tttcctagag gattattgga acttttcgaa agatatttaa atagaacctt
    21361 taatgccgtg atatttctat tgatagtaga agtagataga cctaagttat tattatcttt
    21421 gagcttagtt tttgttgtct ctttttttac attttctttt gtgcgacgtt tcaaatgaga
    21481 tttgtacaag tttaggtcat aggcagttaa ctgtcttatc gaggtgatag aagtatcagg
    21541 aacgatagac tcactcagaa gccattcata aaagagctta taatttttta gatattctaa
    21601 agtggttcga ggtgattgat ctttatcctc acgatcttga acaaaatctt ttatataatc
    21661 gggcatatga agtatgattg tatctatttt ttgatatatt tctgtatttt tcatgtcatt
    21721 catcctttca ataagtaggg gagaagagta tctctataat tggactatac caattataga
    21781 atgttttgtc aattggtatg ctctaaaatt tattaatttg tttcaaaaaa agcatataaa
    21841 caggtatacg tttaataatt taaaataaat ggtatacata aatagggaag gaaatagaag
    21901 ggagcaatct aaattttttg aattttctta attaattata tagaaagaac aatctttcta
    21961 tagagttaga catgagaggg actatgcttg atttaaaatc aacaagcgta tatactgtat
    22021 atacaaagaa aaaggaggga gaagactatg gcaattaaag aaagaaaatt tagggaagtg
    22081 gggaactcag tagtattaac tatttcaaag gagtttctta aagaaaatgg gttaaatccc
    22141 ggagatacgg tttttttaga tgaagaaaaa cttcgagcgg caattacaaa aaaacaagcc
    22201 ttatctgatg aaattgattt agcaatatct caagcattaa ctgaatatga tactactttt
    22261 agagagcttg tcgataaatg agtattcatt acctagaagc caaagatatt ataaaaatga
    22321 atgtaatcca aataaaaaaa tactctccta atgaacccct tggtgtaaag gatgcgaatg
    22381 ctttagaaat gtgtgtaggg caacttcgag cctcagcttt tggtgaagag gtttatcctt
    22441 cgatttatga aaaagcagct atcctactta tacaattgat aaagaaacat ccttttcaca
    22501 atgcaaataa acgtactgct ttcttagcta cttttgtctt tttgaaaatc aatggacagc
    22561 ttttagctat tgatcaaaaa gatgctgtaa atttagcggt atatattgct acgtatgata
    22621 aggatttcga tcaactaaaa tatgaagtga tcaacactat aaagacacat acgcagccca
    22681 tttaaggagt tactgtatgt gtctttatag ggttattagc tgctatagcg aaataattga
    22741 agtaaattac aaatagctat aattcaaatc caactatata gaaaacgtaa attttctata
    22801 tagttggatt aagataacga ctatttattt tatcatattt ttactatacc ctatactttt
    22861 acttggtata tttatgaata atcgcaaatt tacccctata ctactagttt gtaagttaga
    22921 ttaataaaaa tactaaagtt atttgttctt ttattaaata cacctacaac ttataaattg
    22981 atggtattta gataacacga aagaataaaa ataacagctg atagtaaagt ttgttgacga
    23041 ttgctactca atcgaaagtg agttagtgcg tatgaacttg cttctgaaaa cttatgaaat
    23101 gaaagacctt ttacatcttg cagaaatcgt tactgggatt aaactaagca ataaaaacta
    23161 atgttcccct cattctttga acttctacta tttgatttat ttattctgat aaatgacgat
    23221 ttcaccttca ggatcttcaa atcccgtata gaatttattt aagatctctt cagtatcagt
    23281 aataggatag gcgataggcc acataccctt tgatggttag aataaactgc tcaaaatctg
    23341 tatcgtatgt tggctttagc atattaacaa aggctatctc caaattataa accataagcg
    23401 ttgaatcgtt cttaacaatt tcaattcttc ctaacccaaa gtcagaagat ataattaccg
    23461 gacttgcctt acccttcatg tgggttgttg agattccata tttaaaggga attgtcggta
    23521 ctatcggcgg tctgaaaaac atcccaaaaa taaaaaaatc tttcccctgc tagtctccta
    23581 agtccctttg tagcggatat ggcaccataa tctcatttta tcgaagtgag tcatggttta
    23641 tctgttggcc aaccaaaatt gatacaatct aacgatgcac cctctacggc attctagggg
    23701 agtgcacttg cattcaccca ataaaaaccg gcagttagca aagaacttat gtagtcgtat
    23761 gttcttactg ttaacataca agaggtctcc tttccacaat tgcttagcag taaacgggaa
    23821 tttacaaagc taatctcttc cattttctta tctttgttct aaaagtccca aatggagcaa
    23881 ctgtatttac atgaataaat ttatatactt cccatacagc cgttttggtt gcctcgtctg
    23941 cccattttct tttgtgtgca gtgaatagat cttcatcact cattgaatca atcatcatgt
    24001 taatatcatc aatattacga ttgagaattt cctctaattc tttcaatggt agatgtgcat
    24061 aggtatttgt aaaccactta tataactctc ctaactgatt ccatttaaat tgatcagaag
    24121 gcgttttaac agataatcca tttctttcat cattttccca ttttagaatt aaattcgtcc
    24181 aaccaacttg atatgctaaa ttttcagcag gtgttctatc cacttcctca actcttttat
    24241 ccttcaattc ttcaggaata gtttcaaatt ctaaaatata tttagtataa gtcctattta
    24301 tttctttttt taattcttct ttatttttat atgttctcat tatattacct ccataaattc
    24361 ctaattgtct cctttaattc atctataatt tcatccacga tattttaatc cgttctacaa
    24421 tgcatatctg atatgcattg actgtctaac tcatatgtga atgatttata aaatatccca
    24481 acgtctcatc taaagaaatg ttgatcgtca gcaaggagct aaaaaatacg aatatatttc
    24541 ttttgtagta aaatcggact ggtttgcata tcattttctt cgtaaatatc aaaatcatgt
    24601 taactactat caattactaa taaaaactac cactttaagc gttaagaact gttaagttta
    24661 gttaagtgag gatagttcac aaaatccatt gttaaacttc acgaaagtgg taaaactcaa
    24721 aatgctttat ataggattac agcatcgctc tttcttcgat tgcgcgttgg gtaaaaaata
    24781 ttatgaagta aaaattgacg atgacacgtt ccttacggct catcagatga aacagctaca
    24841 aaaacgaaat gatcaactcg aagaaaattt aattctaaaa aagcgattgc catattcacg
    24901 cctcgctcga cgaactatta aaggtcaaga tttacctgaa gatcatcctt tttaggaaat
    24961 tgaagctttt tatgttgcac aatgcattta taatactaca atgatgtttt caccagatat
    25021 tcttctaggg tgcggtgtaa tgaaacagat acacttgcaa aagaaaattc accgcgaatt
    25081 tactaaaatt atgaataatt atgttaatct tccgaaattt ttttccaaaa ttaagggatc
    25141 aagctggtgt tatagattgt ttaaggttgg caaaaaaata acttcttttt aaacaattat
    25201 agaaaaccga tactctgtta agttggttga cacggtgtcg atttttatga ctttcttatt
    25261 ctgaaacttc actaacggac agtacctaaa aagtctatag tatggacgtt tacgagtact
    25321 attttatcaa aaatgctgct catttcaact tatatgtttt agtgaactac ccccacttag
    25381 cttggatagg ctacaattaa ctagacagaa aaattaaggt gtgtagacta gaagaaaaca
    25441 taccaggagg aatttttatg tctaagagaa cacgaagaac tttttcacaa gaattcaagc
    25501 aacaaatcgt caatctttac ttagctggaa agccacgtgt agaaatcatt cgagaatatg
    25561 aactaacggc ttcagcattt gacaaatggg taaagcaatc taaaacgagt
//
LOCUS       DAZKLA010000028        24849 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000028 DAZKLA010000000
VERSION     DAZKLA010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.028"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..923)
                     /locus_tag="ACG85N_000993"
     CDS             complement(27..923)
                     /locus_tag="ACG85N_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0223782.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(916..1596)
                     /locus_tag="ACG85N_000994"
     CDS             complement(916..1596)
                     /locus_tag="ACG85N_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0223783.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1597..2379)
                     /locus_tag="ACG85N_000995"
     CDS             complement(1597..2379)
                     /locus_tag="ACG85N_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0223784.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2372..3289)
                     /locus_tag="ACG85N_000996"
     CDS             complement(2372..3289)
                     /locus_tag="ACG85N_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0223785.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3453..3707)
                     /locus_tag="ACG85N_000997"
     CDS             complement(3453..3707)
                     /locus_tag="ACG85N_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223786.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
     gene            4564..7791
                     /gene="fss3"
                     /locus_tag="ACG85N_000998"
     CDS             4564..7791
                     /gene="fss3"
                     /locus_tag="ACG85N_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0223787.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            7904..8218
                     /locus_tag="ACG85N_000999"
     CDS             7904..8218
                     /locus_tag="ACG85N_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0223788.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            8231..8605
                     /locus_tag="ACG85N_001000"
     CDS             8231..8605
                     /locus_tag="ACG85N_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0223789.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            8615..8950
                     /locus_tag="ACG85N_001001"
     CDS             8615..8950
                     /locus_tag="ACG85N_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFQ0223790.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            9033..10373
                     /locus_tag="ACG85N_001002"
     CDS             9033..10373
                     /locus_tag="ACG85N_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0223791.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            10451..11125
                     /locus_tag="ACG85N_001003"
     CDS             10451..11125
                     /locus_tag="ACG85N_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0223792.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            11118..11282
                     /locus_tag="ACG85N_001004"
     CDS             11118..11282
                     /locus_tag="ACG85N_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223793.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            11358..12500
                     /gene="dcm"
                     /locus_tag="ACG85N_001005"
                     /pseudo
     CDS             11358..12500
                     /gene="dcm"
                     /locus_tag="ACG85N_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            12490..12780
                     /locus_tag="ACG85N_001006"
     CDS             12490..12780
                     /locus_tag="ACG85N_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223794.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            13039..14223
                     /gene="mobT"
                     /locus_tag="ACG85N_001007"
     CDS             13039..14223
                     /gene="mobT"
                     /locus_tag="ACG85N_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0223795.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            14220..14357
                     /locus_tag="ACG85N_001008"
     CDS             14220..14357
                     /locus_tag="ACG85N_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0223796.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            15103..17013
                     /gene="ltrA"
                     /locus_tag="ACG85N_001009"
     CDS             15103..17013
                     /gene="ltrA"
                     /locus_tag="ACG85N_001009"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0223797.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            17117..17341
                     /locus_tag="ACG85N_001010"
     CDS             17117..17341
                     /locus_tag="ACG85N_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223798.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            17354..17857
                     /locus_tag="ACG85N_001011"
     CDS             17354..17857
                     /locus_tag="ACG85N_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0223799.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            17917..18306
                     /locus_tag="ACG85N_001012"
     CDS             17917..18306
                     /locus_tag="ACG85N_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0223800.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            18293..20740
                     /locus_tag="ACG85N_001013"
     CDS             18293..20740
                     /locus_tag="ACG85N_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0223801.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            20745..22868
                     /locus_tag="ACG85N_001014"
     CDS             20745..22868
                     /locus_tag="ACG85N_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0223802.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            22865..23869
                     /locus_tag="ACG85N_001015"
     CDS             22865..23869
                     /locus_tag="ACG85N_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0223803.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            23888..24799
                     /locus_tag="ACG85N_001016"
     CDS             23888..24799
                     /locus_tag="ACG85N_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0223804.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
BASE COUNT         8120 a         4339 c         5324 g         7066 t
ORIGIN      
        1 tactagttct cactaatctt gtataattaa accctccgca acttgacgga gatggagaag
       61 atgcccccgt ctaatctagc ggtcatctct cctcccattc cttccaccaa tttttttgac
      121 aggtaaagcc cgattccgct agattccgta tttgtgcggc tggtattttc ggtataaaaa
      181 cgctggaaga ctttttcaac agaggtttgc gttcctggtt tgataggatt ggccactgtt
      241 aatcgaatga atggatcctc ttttgaaaat gtcacctttg cttgtccatc tgcataacgc
      301 aataaattct gtatgatatt ttgcagcact cgcagcaaca tgagcggatc tgctaataca
      361 gttgtattta tcgagtgttc agcgaacgct acctcgatat ttttctcaga aaattgttca
      421 taaaaagaga aaaatacttc ttccacctct ttagaaaaag aaagcgtatg gagatccatt
      481 tctttttgct tcgtctctat tcgagcaaga tcataaaact gatccgtcat actgagtaat
      541 cttgcaacag attgtttgat gatcgttaaa tacttctccc gtttctccgt atcctctacc
      601 tgttccagta attgtacata accgttaata gcagtcaaag gagttctgag atcatgagaa
      661 aggccgacga tcgatagtct gatgttttct tccatttctt gaaatgcttg tcctttttgt
      721 tcattttcat ccaaccatgt attcagtgca tctactaggt ccattagatt cttttctcga
      781 aattcgactg aaagacgacc accatattct tgtcgttcag aaagagtggc gattttctta
      841 gttaatctct tgatttccct ttgcaggaag aaaataaaac ttcccataaa gataacaacg
      901 agaagcaaca gccatttaat caaatctaaa ccccactccc cacaccgtat gaatatacac
      961 agcatgtgcc tttgctagct tccctcgaag attggaaata tgcatattca ctgttttctc
     1021 actatcaaaa tatggttctt gccaaactgt ttcataaaga tttgctcgcg aaaatacttt
     1081 tttcggatgt tctaaaaaaa gcttcagcag atcgaattct cttcctgtca aatggacagc
     1141 cacaccattc acatttacat ccctcgactc tttgtccagt aagatttcct tgtaatagat
     1201 gcgttgcgac agatctttta cttggacaga agtctgatga cgaagttgga tgccgattcg
     1261 taacaaaagt tcttcgatat caaatggttt cgtcacatag tcatccgctc ctgctgccaa
     1321 caaggaaact ttatctcttt gatcgctttt tgctgaaaga acgatgatcg gaacattcga
     1381 tgtctgtcgg atttctgcta acaatgcttc acctgaaagc cctggaagca tgagatctag
     1441 aaggatcaaa tcgacaggat atgtgcttaa caataatttt ccttccgtac ctgaataagc
     1501 atcaagaagt ttgtatctct gttcaagaac ctcctccagt aaggaatgaa cagcttcatc
     1561 gtcttcgata atcaaaatag tttccagcag gaccacctac ttaatgtcag atttttggaa
     1621 aataaacatt cctagagctg atgataccac gattgttact agactgacca gaatcatttt
     1681 cggtgcttgc gaataagttg cttgttgcgc aaagtccgta tacatgagcc acgtttccgt
     1741 aaaccactcc gttagaaatt ccaagccttt gatttttgcg aaaacaccgg gaatgaaggc
     1801 cgttcccatg atataaagaa aagaaagaag caaactgaca ctggtacttc tagtgatgaa
     1861 tgtcaaagcc actggaacag ataatgtccc taatgtcagc agaagcaata caggaaatac
     1921 ttttagaaca taatttccat agtttttgat tccctcaact tcaccaaatc cgaaagcaag
     1981 tgtgtaagca agcatggcta gtcccgtgat caaaaccacg ccaagtgttg tgatcaaaag
     2041 cgtaacgaga agtttcgaca cataataagt cattcgattc cgtcctaaag ataacgtatt
     2101 gcgaatcgta ccgttttgaa attcttcccc aatataaaag cacgcaaatg ctgcgaaagc
     2161 cagaagaatc catgcagcca ttttttctac cattaaaatc cctgttgcac cgttgatgac
     2221 tgtatccgtt gcttggttta attcattgcc gtattttgta tgcatatacc attcgttcca
     2281 agaataaaaa agcggaagta cgaccatcag tcctagtgag acccacattt tacggctctt
     2341 ccatactttt attttttcag cagttaatag attaaacatg tggattgcct ccagttaatt
     2401 gtaagaaata atcttccagt ttttgcccaa ctaggtgaat accatccaca ggaacttttg
     2461 cttctaccaa tgcttgattg atggctgcta cttggtcaat tccttcaaaa agctggataa
     2521 cacggctaga ttgtacaaaa taatccctat aaccaagaga gtcgagaacc gttactgctt
     2581 gttccgtcgc cgaagtgtca atttttataa attgacgaga ttcttgtgcc aattcttctt
     2641 tcgataattc tttgatcaag tgtccttcat gcaaaatacc atagtgagtg gcgatctgtg
     2701 acaattcatc tagcaagtgg ctagaaagca agacggtgat gccccgttct tttaccaaac
     2761 gttggatgat ttctcgcatt tctgccatcc ctgctggatc catcccattc gtcggttcat
     2821 ccaaaatcaa aaattctgga ttcgtgatca gtgcatttgc aatagctaaa cgctggcgca
     2881 tacctaaaga gaaattcttt gctttctttt tccctgtatt ttccaatcgc atcattttca
     2941 gcaggtcttc gatttctcta tccgacacac cgccgttcgc tgcttggata cgtaggttat
     3001 ctcttgcagt taattctgga taaagagcag gggtttcaat cgattgaccc atacgtcgtc
     3061 ttgctgcctg caaatccgtc gttccaaata attcgatcga accttcatca atcgagatca
     3121 aacccataat agttctcata aaggtagact tacccgctcc attttcccca atcaatccat
     3181 agatcattcc acgtttgatt tcgattgata ctttatctaa tgctttggca gcaccgtatt
     3241 ttttggttat accagttgct ttcaagaccg tttcatttcc aaaatccata cttatccttc
     3301 tttcttattg atgagtgaag tatagttgac gggtataaag aaagcagcca gaaatgtata
     3361 aagaaagtgt aaattttcca attgaatgtc aaaaaaaaca atcggacaat acgtgtcttt
     3421 cctattatca ggcagtttgt tatacgcttg tatcagtttc ctgattcttt cttttcgacc
     3481 aaagtcgttt ctctgtattc ctcttcactg aaaatttttg cttctttcag atcaagcttg
     3541 agtaatttta ccagaccggt tataaggata actatagcaa gactccctcc cagaaaaatt
     3601 agactgtctc gcagacgtgg ttgcgttgtc ttccaatcaa agagcgcacc aaacagtgct
     3661 aatccaagtg gactggccac catggtaatc gttgttatcg ttgacataag tcgtccttgt
     3721 atatgcgttg gaacttctcg ctgaagccag accatataaa aagcaaaaga aaacattaat
     3781 ccttaaaaac ggacttatga aaaataatac atgccgtttg ggatttacaa ttagctacca
     3841 aataagaggt tatgacaaaa gacttttcac aatcccttct tccgaataaa cggtgttctc
     3901 ctgccaactc ctcggtcaca agtcacaata tttatcaaac atgagaagca atttttgtaa
     3961 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcagaagc ggataaataa
     4021 tattgtttga tgatacttta tctttttccc gtttgtttat agatcgaatg atattataga
     4081 ccaagaatcc tacctattaa catagctaaa aacacaaaaa aggcactttc caaattgtgg
     4141 aaagtgccag aaaagttagg attaaggtaa ctgcgctcag cattgcttcg cccagtacga
     4201 ttctcgtaac tcgtgaactc gaacgataat cgcaattaac gttttgagaa ctgtgaagct
     4261 ttacgggctt tcttaagacc tggttttttc ttcgtatcat tgaataagtt tgaaaaacct
     4321 tgatttataa aggttttccc gtaaatatat tttattatat ttgattaaat ttgaaatgtt
     4381 tgtgttctaa aatgtgttct aatttaaagg cagtatattt cgtattttag attaaatttt
     4441 taggctcatt cattctcaaa tccattctat tcagtcaata aaaattttaa aaagacttta
     4501 taaacaaaga tgttcaagcg agcatcttat ttttttgtgc ttaatttgaa aggagaacat
     4561 ttcatgtata aaaaacaaaa aaagaaatta tgtgtattaa ctattttgag tttggctgtt
     4621 caagcaattg ttggagcaat tgctcctacg attacttttg ctgatgaaat tacccatcca
     4681 caaacggtaa ccgtagaact agatttagca catcaatatg ctgttgaagg aacttttagt
     4741 gatggtcgtc caatgtcaga agtcactgtt ccgcattatg cagtgtacaa cggctttaaa
     4801 caagatattt tctgtattga accaggagtc ccgatttaca atgagttcac tcctggctat
     4861 gagaaaaacc cgttaccaga tatgtccgat aaagccaaat tagtttccgt tttatggaaa
     4921 aaagtaggta cagatgcaga tactcatatt gtggcacaga agatgatctg gcaagaagtt
     4981 aatgggtata cccttcactc aataaaacga tcagatggta gtgccgtaaa tatcgcagcc
     5041 attgaggcaa aaataaatca agccattgac gattatcaga agaaaccaac cttccataat
     5101 agtacggcta aaacagtatt agggcaatcg actactgtga cggatacgaa taatctgaat
     5161 ttgtcagagt tcgatgaggt tgtggaaaat gccgcaaaca ttgattatcg tgtgaatggt
     5221 aatcagttga taatcacccc aaatgccaat tcaaaagaaa gtggtgtgct gacgcttaag
     5281 aaatctgctg gtaccggaac acctgtcgcc tataaaaagg ctggccaaca gactttaatg
     5341 gccggggcga ttgataaacc taatacctac acaatcaaaa ttgatgtgga aacggagggt
     5401 tctttaaaga tcaaaaaagt cgataaagaa tcgggtgatg ttgtaccagg aacggttttc
     5461 catttagact ttggaaaaac tttacctgca aaagacgtga ctactgacaa agaaggcatt
     5521 gctacattgg atgagattcc tcatggtaca aaagtaacca ttaccgaaaa atcagtaccg
     5581 gcgccttata cgattgatac cacccccatg actaccacca ttaaagcagg cgaaaccatt
     5641 tacgtgactt caaaaaacgc acgggaaaaa gggcaaatca ttctggataa aagcggagtt
     5701 gaaacaggga gtgatctttg gaatgacaat tattccttag ctggtaatac gtttgccatt
     5761 cgcaaagaca gtccaaccgg tgaaatcgtt caagaaatga ccacggatga aaacggtcat
     5821 gcggaaacac caaaagagat tgctaatgcg ttggaattgg gaacctatta tgtgaccgaa
     5881 actaaagcta gtcatgggtt cgtgaatacg ttcaaacctg taaaagtcga attgaagtat
     5941 gccaatcaaa ccgtagcccc tgttaccagt aacgtaaaag ggcaaaatca agaagtcact
     6001 ggcgaaacca ctttgacgaa agaagacaaa gatactgggg ataaagcaca aggtaaggca
     6061 gtctttgagg gtgctgaata caccttgttt actgctaagg atggaaaagc tgttaaatgg
     6121 agtgaggctt ttaaaccaga gttagcgaag ggaacgaaag gttctgatga aacggtcact
     6181 ttgactttag acgaaaagaa ccaagttgcc gttaaacatt tagctatcaa cgagtattat
     6241 tggcaagaaa caaaagcgcc cgaaggatat agccttgatg aaacaaagta tcctgtatcc
     6301 atcaaaaagg tggatgatag cgaaaaaaat gccgtgatta cccgaaatgt tacggcaaaa
     6361 gaacaagtga ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgcgggcact
     6421 accgaaactg gcttcaatga tctgaccttt aaagtgtcgc cattggaagg gaccaatgaa
     6481 atcacaggtg ccgaagatga agcgaccaca gcttataacg agcaattagg gtttgatggt
     6541 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaagcccca
     6601 gaaggttttc aaaagattac gccattagaa atccgttcta cttttaagga aaacaaagaa
     6661 gactttgtga agagtgagta tgtctttacc attaccgaac aagaccaaaa acaaccaatt
     6721 aaaacggtga ccgttcctta cgagaagctg acgaacaaag cattttctgt tagtttgaac
     6781 cgtttaatgc tctatgattt gcctgaggaa gaagatagtt taacttctct tgcgacttgg
     6841 aaagacggaa acaaagaatt aacttctctg gattctaccg agctagttga taaattgagt
     6901 tacaatctac atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggat
     6961 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtgat tgcagaaaca
     7021 agcgcaacgt tggcgaacaa agagaaaaca ggaacttgga aaattcagca taaattaacc
     7081 gccgaacaag ttttaaataa aactattgtc ttattcaact atgtgtatga aaataaggaa
     7141 gcttttgaag caggcgataa gccagtagcg aaggatgtta gcttgaataa ccaagcccaa
     7201 actgtcagtt gtacagtgga acaccatgtt tctatccaaa caaaggccca cctagaaaac
     7261 ggttctcaga cctttactca tggtgatgtg gtggatatgt ttgatgatgt gtcgattact
     7321 catgatgtac tggatggctc aaaagaagct ttcgaaacaa ttctgtatgc tttattaccg
     7381 gatggtacaa acaaagaaat ttggaaatct ggcaagattg attatgaagt aaatgataaa
     7441 gaattcacca aaactgtgct tgcgaaaaaa gtagataccg gaaaatatcc agaaggaacc
     7501 aagtttactt ttgcggaaat caattacgat aaagatggga atatcaatgg aaaacacaat
     7561 gaagatttga aagaaaaatc tcaaacctta acaccaaaag aagttccaac catactaagt
     7621 acgccgaaac aaccggaaac accaactgtt ccaagtggct ctcaagaatc tagtcccaca
     7681 gtgaagacct tccctcaaac cggggagaaa aattccaatg ttttactgtt cattggtttt
     7741 actttgattt ttgcgacggc gggctattat ttctggaatc gccggaacta aggtgatgag
     7801 atgatacaag taaatcctaa gaaccaacgt gcgcgaccgc ctcctaaaaa agtagtcgcg
     7861 ctatttttgt atccaaaact aacaatgaaa agaggaaaag aagatggaat tgaaatttgt
     7921 cgtgccagat atggctgaaa cctttggaaa gttaagttat gccggtgaag gagaaattat
     7981 aacagaagga tatggacgga atactacggt gattggtcgc agttatcatt tgtattccag
     8041 taagcaacgg gccgatgata ttgaggtggt agtagctgcg gaagccggtg aaaaggattt
     8101 tgatcaagac caaccgctaa aagccgtgaa tccccatttg gttgccaaag gctatgagat
     8161 tgagaatcgt gggtttactg attacgtgtt gtatgttgat gatttagtga aagcatagga
     8221 ggaaaagaag atgagattaa cagaaggtat tgtcgtagat tcaggtttaa catttgggaa
     8281 gttgcgtttt tccgcattac gccgagaagt acggaagcaa aatgaggatg gaacaattgg
     8341 taatgaggta aaagaacgaa cctataattt aaaatcctct gcacaagggc gaatgattca
     8401 agttagtatc ccagctaatg tacccttacg cgagtttgct tatgatgcag aggtagagtt
     8461 ggtcaatcca atcgtggata ccgttgccaa ttatgttttt cgagaaggaa ctaccgttaa
     8521 ttggttcatt aaggcggatg atttagtctt gaagcggcag ccgaatcaag gaaatcctac
     8581 gaaccaaaat gaaagaaaga aataggtgaa gattatggaa attaatgttg aacatatttt
     8641 agattgtctc gatcaatatg gaaaaggcga gctgactgag gaacaactcg ttaaagcatt
     8701 aacctctgat gaaaaaatgt ttctgattat acatcaaggt ttacttggag aaaatagcga
     8761 cagtgaagaa gactctgatg tactagactg gttaggagaa gaagagtctt tctttatagt
     8821 tgtcgaagta aatgaaaact tatgtcggca ggcagaatcc attttggaag aaattggtgt
     8881 ggagatgccg gatgctattg aaggcttttt aaatcaattg gttgaaacga aacaactccc
     8941 ctcctcgtag cagtaaatga ctagcataac tttttgaaat tgcctaatga aatcctattg
     9001 ggctttttcg attggaggtg gaaaccatca aaatgtacaa aggacatcgc atacgcgcag
     9061 gagatcagca cttggtttat cactttgttc ttggctggct actcgctctg ttcatcggtt
     9121 ggatgagcgt cttttatttt caagagttta gacaatttga tatttctaag ctatcacttt
     9181 ctactattga aatcgtttgg tctataaaag atttagtatg tctgcttgga agtctcgctt
     9241 tttcaggagc tatgatattg ctttatattc acttttttct tgatcattgg cgaagcttgt
     9301 ggcatcgaca aaagctagct cggatgattc tagagaacca ttggtatgaa gtgaaacaaa
     9361 ctcaaagtga aggctttttc aaagatttga atagtagccg aaccagagag actatcagtt
     9421 acttccccaa aatctgttat cgaatgaaag atggattact gtctattcgc gttcaaattt
     9481 cacttgggaa ataccaagat cagcttttaa aattggaaaa gaagttagaa agcggactgt
     9541 attgtgagtt agtagaaaag gaactcaaag actcctatgt ggagtatact ttactgtatg
     9601 atatgatcgc caatcgaatt gggatagacg aagtagtggc agaaaatggg actctgcgat
     9661 tgatgaaaaa tcaagtatgg gcttatgatt ctttacccca tatgttaatc gctggtggca
     9721 cgggtggagg gaagacctat ttcctcctca ccattatcga agcactattg aagtcagatg
     9781 cagaattgtt tatcctcgat cccaaaaatg cagatttagc tgatttaggt acggtgatgc
     9841 ctcatgttta ttcacaaaag gaagaaattt ctgcttgtgt ggaagatttc tatgaacgta
     9901 tgatggctcg tagcaaggca atgaaagaaa tgtctaacta caaaacagga gagaattatg
     9961 cctatcttgg acttccaccc aactttttaa tctttgatga atacgtggct tatatggaga
    10021 tgttaacgac gaaagaaagt gcggtgattt tgaataagct caaacaaatc gtgatgttgg
    10081 gtcgtcaatc tggtttcttt ctgattttgg cttgccaaag gccggatgcg aaatatttag
    10141 gagacggaat ccgcgatcaa ttcaactttc gcgtggcttt gggtcgtatg agtgaactcg
    10201 gatattccat gatgtttggg gaggtcgaaa aaaacttctt tatgaagcac atcaaaggtc
    10261 gaggttatgt ggatacaggt ggcagtgtga ttagtgagtt ttatacgcca cttgtaccaa
    10321 aagggtatga tttcttagat tcaattagta aattagaaaa gatgaatgat tgagtgatct
    10381 tcaatactga ctttatactt caagtaacgt ataatacttt tatatagtag gaagaaaggg
    10441 gataattaat atggaaagta atgattctta tttagagtat gcaaaagcaa ataaggataa
    10501 gtttatttct tatattatca aagggaaatc ccccgaagtt ggacgagacg cagtttttat
    10561 ggcagggagt cctggggctg gaaaatcaga ggttgccatt ggattagctg agaattatga
    10621 taaccatgtg attattgatg cggattactt tcgcacccag tttcctgatt ataatggaaa
    10681 aaactctagt ctctttcaaa aagcatcttc ttggttggta gagcaggcat ttagatatgt
    10741 cttagatcat ggctattctt ttattctgga tgcaactttt gcaatattaa gtgctgaaaa
    10801 aaatatctcg cgggcgttga aaaaacattt tcgagtgacg attttctatg tttatcaaga
    10861 ccctaaaatc gcttggaaat ttacacaaga acgtgaaatt gcagagggac gtcatgtgcc
    10921 gaaaaaggca tttattaacg cttttttcaa atcaagagag aacattgcaa aagtaaagag
    10981 taaacatcca gatgtattgc ttcatatcat aataaaagat tatacgaata atatttccga
    11041 agttcattat gctacggata atctagaatt atttttacca ctagggtata gtaggaaaga
    11101 tttggaggtt caattacatg actgagaaag aacaagtaac gaaaattgta aagaaataca
    11161 ataagagcat tgcggattta tcggaaaatg ctactgcaaa agagttcaaa acggtgatta
    11221 aatatgtagc cgatcaggcc aatgaaaaac aacggaaatt agttggatta aataaaaaat
    11281 agtaggacaa attaataatg tatgagccaa gagcaaaatg ttcttggctt ttttctttgc
    11341 aatgaatgag gtaatgtatg agatttttag atttattcgc cggtattggt ggctttcgtt
    11401 taggtatgga acaggcgagc catcattgta tcggtttttg tgaaattgat aaatttgcta
    11461 gacgtagtta taaagcaatt catgatacaa gtaaggaggt ggaaatgcat gacatcacaa
    11521 gtgtatcaga tgagtttatt caatccctcg gaccagtgga catcctttgc ggcggatttc
    11581 cgtgccaagc tttttcaatt gcgggaaagc ggcaaagatt ttccgatact cgaggtactt
    11641 tattctttga aatcgctcgg ttcaccactc ttctccgacc taagctttta ttccttgaga
    11701 acgtccgagg attgctcaat cacgaaggag gggctacgtt cgagacgatc ctccgaacgc
    11761 tggatggatt ggggtatgac gtggagtggt aggtgcttaa ctcaaaggcc tacattcccc
    11821 aaagccggga gcggatattc cttatcggac attctcgaga cgcatgtacc gaacaagtat
    11881 ttcctatcat tggaccgtct tcaacatctg atcaaaacat cagaaatttg gtaaatatca
    11941 atccttccaa tcgaggaatg ggtggacaag tctatggttc agatggcgta gcgccaacct
    12001 taacaggtga tgaaggaatc aaaatcgcac tgccagcaag ggatggaatc tctgtagcag
    12061 gactattacc gggaaagttt gaacaaggta atcgagttta tgaagttact ggaaccgccc
    12121 ccaccttgtc aacgaaacaa ggtggtacaa aaatcatgat tcgaaaaaat gaaacagaat
    12181 atcaagaggt gaagccagaa gacagtgtga atctggcttt tcctaattcc acaagtcgcc
    12241 gaggtcgtct gggaaaacag agtgttcata ccttactaac aggagaccaa caagcgatcg
    12301 tcacggatca gtatcaaatt cggaagctaa cacctagaga gtgttggcga cttcagggct
    12361 ttcccgattg ggcttttgat cgagattctc aagtcaattc agatagtcag ttgtataagc
    12421 aagccggaaa ctctgtaaca gttccggtca tttttgatat tgccagaaga ttaaaagagt
    12481 tggatgtaca tggtttatga tgagttgatt ggagaaattt attgggtgat tggaaagatt
    12541 cagtcagacc cagaattaga agaacaattg cgacggttaa aatttgatat tcgcaagaat
    12601 ggtgtcaaag tcgctggcga tccatattta atgaatgagg agacggatgc tcgaattgaa
    12661 ataaatcaag taattgcaga atttgagcgg attgcggacc aagcaaaaga accagatatt
    12721 cgtcaatact tgttagaaat gaaagcagag ttggaagtaa atggtattac agatgagtaa
    12781 cctaaaccaa cacaactgac tgttaggcgc tgaccgcgga ggattcgcga agcagaaatc
    12841 ctccgtggtg ctgtgcctgt tcatggacgg cgttagccga ccatgatgtc tcacccccgt
    12901 atctaacagg ggggtacaaa aacacaaaaa ataagtaagc aaccattaag aatgcttgtt
    12961 gtatcaaaga tagtaagtaa tttgtatagg tgttaactca tttgaaaaag ttaacacctt
    13021 gaatttggag gtgagaactt ggcacaacgg aatttagatt atcggctatt aaaagaccga
    13081 cgcaacgaat acggcgtttc acaaaataaa ttggctacgg cttgtggact tagtcggccg
    13141 tacttaaatc agatagaaaa tggtggcgta accgcatcta taaaaaccat gaggaaaatt
    13201 tttgaccaac tggaaagctt taatcccgat ttgcctttga cgttactgtt tgactatgta
    13261 aggattcgct ttcccacaac ggatgcacga aaaattattc aagaaattct ccatttgaaa
    13321 tttgagtata tgctccatga agattatgcc ttttactcct atcaagaaca gtatgtcatg
    13381 ggtgatattg tggtgatgtt gtctcatgaa gaagataaag gtgtgctttt agaattaaag
    13441 gggcgaggtt gccgacaatt tgaaactttt ttactcgctc aaaagcgtag ctggtacgac
    13501 tttttcgagg attgtctaaa ggccggtggc gtgatgaaac gcttggactt ggcaatcaat
    13561 gatcgagtgg ggctattgga tatacctgag ctaacgaaaa aatgtcagaa ggaagaatgt
    13621 atctccttgt ttcgtacctt taaaagttat cgttccgggg aacttttaaa ggctgatgaa
    13681 aaggacggca tgggcaatac cttatatatc ggcagtctca agagtgaggt ttatttctgt
    13741 ttgtatgaaa aagactatga acaatatatc aagttaggga ttccattaga ccaaactgag
    13801 acgaaaaatc gttttgagat tcgactgaaa aatgaccgtg cttttcatgc gattcaagat
    13861 ttgttaaaag gtcgtagcat tgagagcacc acgttttcca ttatcaatcg ttacttgcga
    13921 tttgcggata aagtagaagg taaaaggcga accaactggc ccttgaatga acaatggggg
    13981 cgttttattg gacggaatcg gaaagagatt caattaactt ccgagccaaa accgtatacc
    14041 attgaacgaa ccttgaattg gctaggccga caagtcgccc ctacgtggaa aatggctaag
    14101 gagttggatc gattgaatcg gacaacctat atccaggata tggtacagaa tgcacgctta
    14161 tcagaccgac ataaaaagat tttggagcaa caaagtatgg caattgaaaa tttgattata
    14221 tgaaaagagg aatcgtcatg ggaattgtaa aagatatttt tttggtattt ggtggcagta
    14281 tgcttggtgt tactgtgatg tgcttgatga atgcaagtgc agcagcggat cgagcaatgg
    14341 aaaagaaagg aaagtgacaa gatgaatttc ggacaaaatt tatgtgcgac tcgttcctta
    14401 gtgaaaagct aaggcactaa taaaagaacg aaaaagtttg gttattagga gaactgataa
    14461 tctgacaggt ggaatgatga agtaacgctc tgaaacgcct gccctgagcc tccgacaagc
    14521 attgtatgag gaaactcaga atgtttgaag ctcggtaaag acggctgaaa gataccgtaa
    14581 tagttgatga acatatatct tctatcgaaa actatccaga ggtgggtagc gtatccaaca
    14641 ggtcgggggt ctataaaata tctatggtta gaatgttcta taaggaactg acgaaacagc
    14701 gaatgtacgg gtctaaattt gaactttgta gaaatgcaaa ggtacgagaa atcgtagctg
    14761 gtgtggcaaa gtaagaatat aggttatgaa atgccatata acgttacagg cggtatcaag
    14821 ccagcaggct tatagctagt gacctaagga tatatatgta tagataagat tatcggaacg
    14881 aggaaaggta ttgggttctc atcaagagaa taaactgacg aagaacaata agcagttgaa
    14941 ctaatgctga aaagcagagg gcgaacctaa gaaattcttg taatgagaat ggaggaatgc
    15001 cctcaagtcg tgtatatatt agaaaagtat tatgttgttt caataaggat tacgagtaag
    15061 ataaaaaagc tcactcaatt tcaagtaaag gagtgaagcc ttatgcctaa aaataaaaat
    15121 gaaacattgt gtgtagaaga tttaagacac gctgaatatt acgaaatgca aaacaccttt
    15181 gatgatttat atgccaaaag taagaatgga gatattttca ctcatcttat ggatattatc
    15241 ctatcgagag aaaatatatt actggcatat cgaaatatca aagcaaatgc aggcagtaaa
    15301 acagcaggaa cagacggtac aataattaaa gatattggca aattgccagc agaaacagtt
    15361 gtcaaaaaag taagatacat tgttgcaggc agtccacatg ggtatcgacc taagccagtg
    15421 aggcgaaaag aaataccaaa accaaatggt aaaaccagac ctctaggtat tccttgtatg
    15481 tgggacaggc ttatacaaca atgtataaaa caagtattag aaccaatctg cgaagctaaa
    15541 tttagtgaaa acagctatgg ttttagacct aataggtcag ttgaaaatgc aataaaagca
    15601 acctataacc gcctacaaat cagccagcta cattatgtga ttgaatttga tattaagggt
    15661 ttctttgaca acgtgaacca ttccaagctg ataaaacaga tatgggctat gggaatacgg
    15721 gacaagcact tgatattcat tttaaaaagg atattaaaag ctccaatcaa aatgactaac
    15781 ggtacaataa catatccaga gaaaggaact ccgcaagggg gaataatatc cccactacta
    15841 gcaaacattg ttcttaatga gttagaccat tgggtagaaa gccaatggca ggaaaatcct
    15901 gttacaaaaa actatgtggt tcatatcaat aaaagcggta gcccatgtaa aagcaacgct
    15961 tacaaagaaa tgaagaaaac aaagttaaag gaaatgtata tggttcgtta tgcagatgat
    16021 tttagagttt tctgccgcta caaggaaagt gctgaaaaag ccaaaatagc aatcactcaa
    16081 tggattgaac aacgattaaa attggaagta tcacaagaaa aaacaagaat tgtaaatgtt
    16141 agaaaaagat actccgactt tcttggtttc aagattaaaa tgataccgag aagaaagaag
    16201 ttagttgtta aatcacatat aagtgataaa caattcaaaa accaaaaaga caagctggta
    16261 tcacaggcaa agaaaattgc ttctccctca aaagggaaaa cggaattaga agaaacaaga
    16321 ctttataatt caatggtatt aggtatgcaa aattattatc agattgcaac gaatgtaaat
    16381 ctggattgta tgaaactgaa tagagctgta atggctatct tcactaacag attaggtaca
    16441 aacaaaaggg gcagattgag aaaaacaggc agacaactta cgacaaagga ggcaacaaga
    16501 tatggtaaat ctaaaatgct tcggtatgtt gctggcgagg gcgaacctat tttccctgtt
    16561 ggatatatcc aacacagaaa accaatggca aaggtgtatt tagcgaattg ctacactcta
    16621 gagggaagaa aatttatcca caccaatcta agtgttgata aattactgat gtaccagtta
    16681 atgaaattat ctttggaaaa tcgcacaata gaatacgcag acaatcgaat ttcgctattt
    16741 tctgctcaac atggaaaatg tgctataact ctggaagaat ttcaacaagc taatgaaata
    16801 cattgtcatc atattgtacc aactggagct ggtggaaatg acgattataa aaatcttatt
    16861 ttggtaaaag aggcggtaca ccgtctaata cacgctaaaa cagaagaaac aatacaaaaa
    16921 tatatagctc tattgaagct aaataaagtc cagctcataa gactaaataa atatagagta
    16981 ttagctggca atcaagaatt aaacttgata taggtctact acataaatac ttttcaatat
    17041 aaacacgatg gaacgcggag tgcggtgaaa gtcgcacgct ccgtgtgaat ggggggaaaa
    17101 gtttgagata tttataatat caggaactta cctatccata tatcaatggt ttttaaccaa
    17161 tgcccaatcc ttagtcttac tggcaattgt ggtcattggt ttgtttttag gatttaagcg
    17221 ggagttttcc aaactgattg gctttttggt gattgctttg attgcggtcg gattggtctt
    17281 taatgcttct ggggtgaagg atgtcttact cagtttgttt aaccgaattg tcggtgcgta
    17341 agggggaagc acgatggaac agatgcgtgt ttatattgcg aacctcggga aatataacga
    17401 aggtgaattg gtgggggcgt ggtttacccc acctgtcgat tttgatgaag tgaaagaacg
    17461 aattgggtta aatgacgaat acgaggaata tgcaattcat gattatgaac ttccctttga
    17521 gattgatgag tatacgccaa ttgaggaaat taatcggttg tgtggcttag cggaggaact
    17581 tgaaggaacg ccagttggtg aagttgcttc tgagattcaa catgctttct tcaattcttt
    17641 tgaggagatg gtagagcatg tggatgatat tatctattat ccagattgcg atgatatgag
    17701 tgatgtagca tatttcttga ttgtagaagc tggtgatttt ggagaagtac cagaacactt
    17761 gaagacgtat attgattttg atgcgtgtgg tagagacttg gagattggag gcaattattt
    17821 ggtaacgagt cgaggtgttt ttgagtatcc atattaacaa aattattgaa gggtagttga
    17881 tttctaaact atccttcttt ttaaaggaat gatttgatga agaaaataaa aagctacacc
    17941 agtatttggg cagttgaaaa ggtcatttat gcaattaatg actttcaact gccttttcct
    18001 gtgactttta accagatggc ttggtttgtt ttatccttgc tgtttgtcat cgtatttgcc
    18061 catgtgcctc cactatctat gatagagggg gcctttttaa aatacttagg tataccggtg
    18121 gcagttactt ggttcatgtc gcaaaaaacg tttgacggta agaaaccatt gggattttta
    18181 cgatctttta ttagctatca cttacgattt aaggtcactt tcgcaggaaa gaaagtcaaa
    18241 gaacagaaga aacgatggga cgaacccatt actctggtaa ggagtgtgaa ctatgtaccc
    18301 gattaagtat attgaaaata atcttgtctt taatcaagag ggagaatgtt ttgcctacta
    18361 tgagttaata ccttacaatt actccttttt gtcacctgaa cagaaatatc aagtccacga
    18421 taactttcgt caattgattg cgcaaaatcg tgaaggcaag attcatgcct tacaaattgc
    18481 cacagaaagt agtattcgtg ccacgcaaga acgatcaaaa aaggaaatta ccgggcgatt
    18541 aacagaagta gcgaaacaac gaattgattt acagacagag gcattagtat ccatgattgg
    18601 cgatagtcag attgattatc gcttttttat tggcttcaaa ctgattgcga cagatgaaga
    18661 ggtcaatcta aagaatctca agaaatcttt cttctccggc tttcaagaat ttgtctatgg
    18721 agtgaatcat catttgatgg gcgattttgt ttccttatcc aatgaagaaa ttcgccgata
    18781 taccaagctt gaaaaactaa tggaaagtaa attggctcga agatttaaag tcagacgagt
    18841 cacaccgagt gatctgatgt atttgattga acatatctat ggcgaaaagg gtacaccatt
    18901 tgaagaatat gaatttcagc taccaaagaa aaagctgaag tcggaaactt tggtgaaacg
    18961 ttacgactta ttacgtccca gtcgttgttt gattgaagaa aagccccggt acttatgcat
    19021 ggaacatgag aatcatgaat catatgtagc gtacttaaca attaatacaa ttgtggggga
    19081 aatggagttt ccctcctcag aacttttcta ttaccagcaa caacaattta cattcccaat
    19141 tgacactagt atgaatgtgg agattgtgac gaacaaaaag gcacttgcta ccgttcgcaa
    19201 taagaaaaag gaattaaaag acttggacaa ccatgcctat caatctgaca atgaaacaaa
    19261 ttccaatgtc ttagatgcat tggattccgt agatgaattg gaaactacct tggatcagtc
    19321 caaagaatcc atgtacaaac tgagctatgt ggttcgagta agtgccgaat cagtagatga
    19381 actaaagcgc cggtgtgatg aagtgctaga tttttatgat gataccaatg taaaactagt
    19441 ccggcctttt ggcgatatga tggggctaca tgaagaattc ttaccttcta gtaagcgata
    19501 catgaatgat tatatccagt atgttacgtc tgattttctc gccggacttg gttttggcgc
    19561 cactcagatg ttaggggagt tggaaggcat ttactttggt tataacgtag atacggggcg
    19621 caatgtttac ctgaaaccgg cattggcctc acaaggggtc aaggattcag ttaccaatgc
    19681 gctagcagcg gcattcctcg gttcactcgg tggcggaaag tcctttagta acaatctctt
    19741 agtttattac gccgtccttt ttggtggaca agctgtgatt gtcgatccaa aaggggaacg
    19801 gggtggctgg aaagaaacct tgccagaaat tgcggatgaa atcaatattc tcaacttaac
    19861 tagtaagcca aaaaatcaag gattacttga tccttatgtg attatggaga aaaagaagga
    19921 ttctgaaagt ctagcaattg atattctgac ctttttaaca ggaatttcta gtcgagatgg
    19981 ggagaagttt ccagtgttgc gtaaggccat tcggacggtt acccaaagca atcaacgggg
    20041 attattacaa gtaattaatg agcttcgaaa agaaggctca ccagttgcgg aaaatatcgc
    20101 ggatcacatc gagtccttta ccgattatga ttttgcccat ttgcttttct cagatggttc
    20161 gattacccag tcgattagtt tagataggca gttaaatatc attcaagtag cggatttggt
    20221 tttaccagat gcagaaacca cttttgaaga atataccacc atggaattgc ttagtgtttc
    20281 catgctaatc gtcatttcca cttttgcctt ggactttatc catagtaatc gagaaatctt
    20341 caaaattgta gacttagatg aggcgtggag ttttcttcaa gtagcgcaag ggaaaacatt
    20401 atcgaataag ctcgttcgtg ctgggcgggc tatgaatgcc ggggtttact ttgtcacgca
    20461 aaatagtgat gatttactgg atgaacgctt gaagaataat atcgggttga agtttgcgtt
    20521 tcgcagtacc gatattcatg aaatcaagaa aaccttggaa ttctttggct tggatcgaga
    20581 agatgaaagc aatcaaaaac gcctacgaga tttggaaaat ggccaatgct tgatgcagga
    20641 tttgtatggt cgggtaggcg tggttcaggt acatccagtc tttgaagaac tcttccatgc
    20701 ctttgatacc cgtccacctg ttcaagagag aatggagtga ggggatgaaa aagaaacttt
    20761 ttcggattgt gggaatcgtt tttattagca tactcactct gatagttcta ttgagtttag
    20821 ttggtacggt agccgaggcg acagggctag ttgatgatac agttgaagca ggaaatctat
    20881 attccaaata ttcactgaac aactatcaac tagatttctt tgtggatagc tcttgggatt
    20941 ggttgccttg gaattggggt gatggcttag ggaaaagcgt gatgtatggt ctttatgcca
    21001 ttactaattt catttggacg gtgagtttgt atttgtcgaa cgccacgggt tatgtggttc
    21061 aagaggctta caaattagat ttcatttcgg atacggctga aagtattgga aagaatatcc
    21121 aaacattggc tggtattact gaaaatggac tccagacctc cggattttat tttgggtttc
    21181 tattattgat gattttagct ctgggggtgt atgtggcgta taccggatta ttaaaacgag
    21241 aaaccacgaa agcagttcga gccgtgatta attttgtgat gatttttcta ctatcgggtt
    21301 cgttcattgc ttatgcgcct acctatatca caaaaatcaa tgactttagc tcagatgtta
    21361 gtgaagcggc ccttaccctt ggaaccgaga tcgttgtacc caattcagaa agccaaggaa
    21421 aagatagtgt ggatttgatt cgggatagtt tgttttcgat tcaagtccaa caaccttggt
    21481 tactactgca atttgatgat tccaacatag aagaaattgg tgaagaccgg gtcaacaaga
    21541 ttctatctgt gagtccagat gaaaacaaag gcaaagatcg ggaagaagcg gtcaaggcag
    21601 agattgaaga caatgacaat gcgaatctca gtattaccaa gaccatgaac cgtttaggga
    21661 ccgtggtctt tttggtattg ttcaatattg gcatttcctt ctttgttttt cttctaacag
    21721 ggattatgtt gttctcgcaa attctcttca ttatctttgc gatgtttctg ccaattagtt
    21781 tcttattgtc catgttgccg acttatgaaa gtttaggaaa gaaggcgatt attcgtttat
    21841 tcaatacgat tatgatgcga gcgggtgtta ctttggtgat tacgactgca tttagcatct
    21901 ccacgatgtt tttcaatatt tcggcaacct atccattttt catggttgct tttctgcaaa
    21961 ttgtgacctt tgccggtatc tatttcaaat tgggcgacat tatgagtatg ttcaacctcc
    22021 agagtaatga tagccagtca atgggaagac gcgtcatgcg aaaaccgcaa atgttaatga
    22081 atcgaaaatt gcggcaactc aatcgaaatg tgggacgagc tttggctttt ggtggtggtg
    22141 cagcagtcgg aaataagttg gcaaaggaac aatcaaaatc caaatttaag ccatccggtt
    22201 cttctctacg aaagaattca cgattaccga acgatcacga aatactatct gacttaacta
    22261 aggagaatcc agtttcaaat aataagaaac aatcccgaat gaatttggct gggagaaaaa
    22321 tcggcaaagt tttggatacc caagctttgg tgaaagataa gactaaacaa gtaaaggatc
    22381 aggtaaggaa tacgccaacg aatttgaaat acaatctcca taaaggaatc gaaaagtcga
    22441 aaaaagcacc agaagaattc aagcgcggac tgttccaaga aaaagcggat cgagtggatt
    22501 tgcgagaaaa acaacgacaa cgaagaaatg cgaaaatgga cgaaaaacgc aagatactga
    22561 gtgaagcaac aaatcgtcag ggaaaaagaa gggcgaatat tccaatagat aaagcagctc
    22621 aacctcagaa agatatgatg ctgaaaaaag aagcaccaaa gcggattgtg agagcaaatc
    22681 tgaatcccga aatcaaacga aaactaacta gtcaagaaat catttcaaaa gggaaaagcc
    22741 aatcagcaag taagaaaaat tctttccaac aagtgaaaca gaggtccact ctgccaaagc
    22801 agaccaatca aaaagtacaa aaaagaatga accatcgtcc aaaatcaagg tcaggtgaga
    22861 aaaaatgaac ttgaagaaag tgggtgttat ttcattaact tttctgacca tctcttttat
    22921 gggtattttg atgtgtgtgg gtttgttgtt tggtgaagac agtgagggaa atggtagttc
    22981 tggcacttct cctggcggaa ctagtgtctc agaagaagta ttgaaacacc gtgcaatggt
    23041 ggaaaagtat gccaaagaat cggatataac agagtatatt ccaattcttt tggcgattat
    23101 tcaagtggaa tctggtggca caatggaaga cgttatgcaa agttctgagt cagccggatt
    23161 accacctaat tcattaagta ccgaggcatc catcaaacaa ggctgtctct attttgcgtc
    23221 tttggttaag cgttccaaag aactagggtg tgatcaagat agtattattc aggcctataa
    23281 ctatggtggc gggtatctag atcatgtcgg gaaaaacggg aagaagcata gttttgcttt
    23341 agctgaatct ttttcaaagg aaaaatcagg tggtcaaaaa gtcgattacc ccaacccaat
    23401 cgcgattaag gaaaatggtg ggtggcggta taattatggc aatatgtttt actgtttatt
    23461 agtgaagcaa tacctaacta caactcagtt tgatgacaaa atagtccaag aaatttttga
    23521 tgaggcattt aagtatgaag gaacggccta tgtgtttggt ggctcaagtc cagaaacggg
    23581 gtttgattgt agtggcttga cccaatggtg ttatgccaaa gttgacctga aacttcctcg
    23641 ggtggctcag gatcaatatg atgccatgac tcatattgat ctaaaagatg ccaaaccagg
    23701 tgatttagtt ttctttcatt caacctatga tgccggtact tatgtgaccc atgtgggcat
    23761 ttacgcaggg gagaatcgca tgtttcatgc aggagatcca gtggggtgga cgaatctaac
    23821 ggaaaactat tggcggcagc atcttatcgg agctggccga tacaaataag agaaaagagg
    23881 aaaaagaatg aagataaaaa ttgaacggaa tcaaaaggaa aagagcccaa aaaagaaaaa
    23941 agaacgagtt gtatccgtgg gtaagcatcg aaaaatggtg cttgcccttt ggcttttatt
    24001 gtcttgtagt cttgcttttg gcgtttataa aaatttcacc gccattgatc aacatacaac
    24061 tcatgaaaaa gtggttataa aagagaaggt agtgaatact tctggcgttg aaagttttac
    24121 agaggatttt gcgaaagaat atttttcatg gaaaaataac aaagaagcaa ttgaaaagag
    24181 aatgagtaat ctcgaacagt accttacaga aaacggtctc gctttaatcc aagatatggt
    24241 tcgagcagat attcccacga gttccgaagt tcaatctgta aaaattctag atgttgaaaa
    24301 acactcggaa gaatttgtcg tttcattttt agtggagcaa catattacag agggaaaaaa
    24361 gacgcaatca atttcttctg catatcgagt gacaatcttt gaagatgaaa acaggaatca
    24421 tttagtaact agtttgccaa caatgattgg aaagccagac aaggcaaaat atgaagcaaa
    24481 acaagtagaa aacgattcag aaattgatgc caaaacaacg gaagaaatca ccgatttttt
    24541 agaaacattt tttaaacttt atccatcagc ttctgaaaaa gaattggagt attatgttga
    24601 aaatggtgcg atgcgaccaa tcaatattga cttaaagttt tcggagttag agaatccagt
    24661 ttatctacaa aacggaaaaa attttctagt gagtacaaag ataaaatacc ttgataatgt
    24721 atcaaaaacc actagctact ttcagtacaa attagtatta caaaaaaatg gaaactggaa
    24781 aatcgttgat gttaaataat aatgactcaa tctaatttta ttagaagtta taagtagtgt
    24841 aacttattt
//
LOCUS       DAZKLA010000029        24447 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000029 DAZKLA010000000
VERSION     DAZKLA010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.029"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..2478)
                     /locus_tag="ACG85N_001017"
     CDS             complement(133..2478)
                     /locus_tag="ACG85N_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFQ0223805.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2757..3659
                     /locus_tag="ACG85N_001018"
     CDS             2757..3659
                     /locus_tag="ACG85N_001018"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFQ0223806.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3870..5645
                     /locus_tag="ACG85N_001019"
     CDS             3870..5645
                     /locus_tag="ACG85N_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFQ0223807.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5711..7297)
                     /locus_tag="ACG85N_001020"
     CDS             complement(5711..7297)
                     /locus_tag="ACG85N_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFQ0223808.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7548..7772
                     /locus_tag="ACG85N_001021"
     CDS             7548..7772
                     /locus_tag="ACG85N_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFQ0223809.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7914..9665
                     /locus_tag="ACG85N_001022"
     CDS             7914..9665
                     /locus_tag="ACG85N_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFQ0223810.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9665..11449
                     /locus_tag="ACG85N_001023"
     CDS             9665..11449
                     /locus_tag="ACG85N_001023"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0223811.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11784..12636
                     /gene="licT"
                     /locus_tag="ACG85N_001024"
                     /pseudo
     CDS             11784..12636
                     /gene="licT"
                     /locus_tag="ACG85N_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            12733..14619
                     /locus_tag="ACG85N_001025"
     CDS             12733..14619
                     /locus_tag="ACG85N_001025"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0223812.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14660..15562)
                     /locus_tag="ACG85N_001026"
     CDS             complement(14660..15562)
                     /locus_tag="ACG85N_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0223813.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            15792..17195
                     /locus_tag="ACG85N_001027"
     CDS             15792..17195
                     /locus_tag="ACG85N_001027"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0223814.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17195..18628
                     /locus_tag="ACG85N_001028"
     CDS             17195..18628
                     /locus_tag="ACG85N_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0223815.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18773..19699
                     /gene="trxB"
                     /locus_tag="ACG85N_001029"
     CDS             18773..19699
                     /gene="trxB"
                     /locus_tag="ACG85N_001029"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFQ0223816.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19912..21639
                     /locus_tag="ACG85N_001030"
     CDS             19912..21639
                     /locus_tag="ACG85N_001030"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFQ0223817.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            21655..22005
                     /locus_tag="ACG85N_001031"
     CDS             21655..22005
                     /locus_tag="ACG85N_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFQ0223818.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(22099..22959)
                     /locus_tag="ACG85N_001032"
     CDS             complement(22099..22959)
                     /locus_tag="ACG85N_001032"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFQ0223819.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23051..23479
                     /locus_tag="ACG85N_001033"
     CDS             23051..23479
                     /locus_tag="ACG85N_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0223820.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23641..23817
                     /gene="rpsU"
                     /locus_tag="ACG85N_001034"
     CDS             23641..23817
                     /gene="rpsU"
                     /locus_tag="ACG85N_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFQ0223821.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23844..24290
                     /locus_tag="ACG85N_001035"
     CDS             23844..24290
                     /locus_tag="ACG85N_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFQ0223822.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7745 a         4365 c         5209 g         7128 t
ORIGIN      
        1 ggtttttcac atttttacat aaatttgtct taatatcgta gatgtaagct tttgaacacc
       61 atttattcgg aaaaagggag ctgtgacaag atttttgtcg cagctccctt taattctgat
      121 taatctatta gatcaatcat ctttatcgtc tgattctttg gtctctggat tcgctctagc
      181 ataagctgaa gcggtgttcc agtaatctgt atagttttca tcagtaccgc ctacgccgaa
      241 tttaaaggcg cttttcttcg gaccattttt tgcccaaagt gactccactg tttttcctgg
      301 cgtttggatg gattttttat tgacagtaac ttttccagct gtttgtcctg tgaacccaga
      361 tacttttttc ttcatcacat cggatgataa gtcaaatttc tcatcagtgc cgaaaatctc
      421 agggtttgct tggtaagctt gattgattag attggcaaga taattagccg aacgtattct
      481 tgcttggctg tccattccag tattgtcatc gtgtcctgcc caactactga tagtgataga
      541 tggagttgag acgatcagcc atgaatctcg gtattcattg gtagaaccag tttttccgac
      601 ccaatctgca tttcctagat ttccgtttaa gcttgagatg acatctttga atggtgtagt
      661 gattttttca ttgatcacac tacgcatcat gtcattcatg atcgatgccg ttgcttcgga
      721 ataaatacgg acagaattac tttcatgttt ataaatcaca ttacctgcat tatctgtgat
      781 ggaatcaatg atatagcctt cttcgtagac tccaccattt gctaaggcct ggaatccatt
      841 tgtctgttga agcgtcgtta catctactgt ccctaaagga gccgattcaa cgccccaatt
      901 atcatttgct gggtagttca tcttcgccaa gtagtgctcg taagcgtatt gtttcgatcc
      961 accggaattc aacacatctt gatataagtg ataagctggg atattgttgg accattccag
     1021 tgactcacgg acggtttgga aggtgttcga cccttcattg gtcgcattga cgattttctc
     1081 accagcattt tttccttctt gccaagtcgt tgcataatct gatacacggg attctgatcc
     1141 gacaagtccc atatcgattg caggaccgta aacaagaacc ggtttgatgg atgaaccagc
     1201 ttgacgttct gtatcaaagg cgtggttatt ttgattctgg ctgtaattcc gaccacccac
     1261 gaagccatag atacgtccgg ttctgttatc catcaataca tttccagtct ccacttggct
     1321 accagcacca tcatctaaca tgtagccata attttctacg ccggtttgta atgcagcata
     1381 gatatcttta tcgatcgtcg aatggatcgt atatcctttg ttttggatcg tttgctgtgc
     1441 tttttgataa taagcttcac tgaccgatga atctgacata tcagcattat ctttttctgc
     1501 caattgttgt gcaataatcc ttgttgcttc gttcatcacg gtataataca agaattctct
     1561 gtcagtctgt tccgcgattt gttgtgggag aaagtctttt gtaagatcat aggctttcgc
     1621 ttcttcgtat tgttctttcg taatttgtcc ttcacggtac atactgaata aaacaaaatc
     1681 ttttctggca agaccggcag aaagatcttc ttttagcgcg cctgtattcg tgtatggact
     1741 atacgtgata gggctttgag gcaaaccagc gatataagca gcttgtggca gagtcacatc
     1801 ctttgcatct acaccaaaga ttcctctagc agcttcctgg actccagcaa tattttgccc
     1861 cttattattc cgaccaaatg gtgagacatt caaatacgta gtaatgatct cattttttga
     1921 aaagtgtttt tctacttgtg cagaaagaac gatctcgttt gctttacgtt tgaatgttgt
     1981 ttcatcggtt aggatctgtt gtttgactaa ttgctgtgtc aaagtggatc ctccagacga
     2041 gccgatgcca gtcgcttctg aaactagtgc tcgtaaaaca gctttaggga cataaccttg
     2101 atgtttatcg aagtattcat cttctgtact gataatcgcg gttttcaata aaggcgaaat
     2161 cttatcagat gaaatagtcg tccgcatcag atctgtctgg atttttgata tctctgtatt
     2221 atctgcataa acaatctttg acgtttccgt aatgtttcct agttctgtct gcagttcttt
     2281 tttagtcggc aattgtgtgt cttcgacaag ataggcaaag tagccagcac ctattccgat
     2341 acctagagct gcgaacaaca aaagaagaac agtaataaaa acagtcaatg attgaaacac
     2401 tctaaaaata atcttaggaa caaaccattt ttcttttttt cttctattct gtttttttcg
     2461 agtatttttg cgtggcaaaa gggtcacctc aactaatggt tttatttcgc taattatagc
     2521 aaataatagg acaaaaaaac agacaaggct gtttttgaca ggaatttctt tttcgaatga
     2581 gataaaacgt gaggaaatga tcttttttat gaaagagttc ttaagctttt ctttagggaa
     2641 agtttcagtt actatggaga acagggcaaa gaaaaacaaa ccattttctt tgtacatcat
     2701 cttatcaata tacaggaatt gttcgaaaca attgcctttc aaggaggaat ccatccatgt
     2761 tattaggctc acatgtcagc atgagcggaa agaaaatgct gttaggcgcg gcagaagaag
     2821 cagccagtta cggatcttcg acctttatga tctatacagg cgctcctcaa aatactagaa
     2881 gaaaagctat cgaagagatg aatattgatg ccggaaaagc atttatggca gaaaacggac
     2941 taacaaatat cgtcgttcat gcgccttata tcattaattt gggaaatacg atcaaaccag
     3001 aaatgtttcc atttgctgta caatttctga gagaagagat tgtccgtgcg gaagctttag
     3061 gtgctactca aatcaccctt cacccaggcg ctcatgtagg agcaggggca gaagctggga
     3121 ttgctagaat tattgagggg ttgaatgaag ttttgacaaa agaacagacg gcacagatcg
     3181 ctttggagac gatggcaggg aaaggtacgg aaatcggtcg aacatttgag gaattagcgc
     3241 aaatcatcga tggagtaaaa ttgaatgaaa aattatccgt tacttttgat acatgtcata
     3301 cgaatgatgc cggatataat ataaaagaag atttttcagg tgtaatggaa gagtttgata
     3361 aggtcatcgg attagatcgg cttaaagtac tgcatatcaa tgattctaaa aatccacaag
     3421 gttctcataa agatcggcat gccaatatcg gttttggaac aattggattt gacacattga
     3481 acaaaatcgt ccatgatcca atgtttgaca acatttcgaa aatattagaa accccatatg
     3541 tcggtaaaga taaaaagaaa gcttacgcac catatggaaa agaaatttcc atgttcaaag
     3601 cgcaagcatt cgatcccgaa gtgttccctg agttattagc agaagaagtc acactttaag
     3661 ttttctacct gacagcaaca aaaaaggatt gggcagtctg ccttgtcctt ttttgtttaa
     3721 gaaacaaagg agtgcttttt gcattcgttt tcgccatgct ccttttgccc cctcttttta
     3781 aattcagaca aacagggtat actgatagaa aagtaaaaaa cgaaaagttt cacaaacaag
     3841 tagcaaaacg tgattgaaag gacgaaaaga tgctgaacat tttaaacccc ttgatcgacc
     3901 aactaaaaaa gaaacataca tactttattc ttggcatagc cgtattattt acaattattc
     3961 ggttgtattt atatagtaaa gcaatctatg gaacagatat gtcggcgacc tatgatgatc
     4021 agctgctgat tcactatgct caaaatattt tggatggtaa atggctaggg gagtatacga
     4081 ctacaacact ttccaaagga atctcctaca gcttattttt agtacttgca cataaactgc
     4141 atctttctta tgcagttttg ttcggcatgt tcagtatttt tgccagtata ttggttgcac
     4201 tcgcgtttaa accgattgtg aagaacaagt atcttctttt aagtatctat ttttattttt
     4261 tgttttctcc gattagtttt acacatgaat attccactcg aacatatcgt aatgccctcg
     4321 tgattccggc tgttgagatc attatcgctt gtttattagc tatttattac cgcaaatacg
     4381 gttctagcag aaaattgctg ccatggtttt tagggctttc tgtcgctttt cctttctttt
     4441 ggttcattcg cgaagattct atctggcttc ttccttttac tgtagtagct ttgggtatca
     4501 gtttcgtcca gatcttgcta gggcaagact tttctttcaa agaagtaaag aagttaaaaa
     4561 aacgactctt tcttctctcg aaacgggaat ggctgaaact ttcattgttt ttacttccta
     4621 tagttggctt ttccatcgtc tatcagtgga tcgaatataa aaatgagaca acatacggta
     4681 ttgcaaccat caatgaccgt tccagtggag aatttggacg tttgatgaaa caactgatcc
     4741 gaatagatga tgaaacgaac ttgaataaag aaaacagtga gatatgggtc tcacatgctg
     4801 cactaaataa agcattagcc gcatctccta cattccatac tgtttctacc cgtattgacg
     4861 aattatatac gactcacgcg tggactcaag gtggaaaagt aaaagaatta cccggagata
     4921 tcatcttttg ggcattgcga gatgtggtaa gcgaatctgg ttattaccaa aacaatgcaa
     4981 aagaaaccaa tgatttttgg aaaaaagtca accaagaatt agaagaagcc tatcacaagg
     5041 gaaccatcaa aaagaaaaaa gagttctatc tgatgggatc aggagatgga aagacgatga
     5101 gtgatctgcc aatcgtattc gatttccttc aatcaggata cgcttatgca tggcgatata
     5161 aagatttcca tcaaggagga aattatagtt tcggaacgca agaacaagtc aaacaggctc
     5221 aagaattatt aaaccttccg ctaatggaga attggatcga tagtaatgaa ccgagaacac
     5281 ctaatttcca attaacaaaa tcagcaaaag tcgcgaatgt catcataaaa atttaccaag
     5341 ttctggctat tcccgtgctg attgcttcaa tagcagggat attacttatg atactaggaa
     5401 ccgtatttgc aaaagctgat cgtgcctatt atagttcctc tttgattgtg gtcaccggat
     5461 taatactgac acaattggtt tttcttttcg gtgtctcatg gttttgttct tatgcgccag
     5521 aaaaaaagga ctatttttta agtgtctaca ctggtgcagg cgtgccatta acgcaattca
     5581 tcgtagtaac aagcatgcta gttttcagta aattaaatct gaaaaaagct ttatcttcta
     5641 aataaaaaga cccttggtag ctagattttt aacctaacta tcaagggtct ttgtgtggaa
     5701 aaatcagttt ttattcgatt tcttctaccg tttcctgcaa aagctgttca tattttttgt
     5761 ttcctttata taaatcacca atcgtccatg ccaacaggag aaggacaaga acaatgatta
     5821 cataagcgat gtgatcagcc aatgctagtt gagcggaagt ggcttgctcg ccgaagaaac
     5881 tttcgatctg tcctggtaat cctcgtaagt tcaagtaagt tagactaatc acagagaacc
     5941 agccaagaag ttttacgatg aatgaatttt taaatcggct acccatctcc gcttggctgt
     6001 tggtcatcat cagtaaagga atcatcgaaa atggcaacgc aaaagccaag aacacctggg
     6061 attcattcat taagttattc aaagcggcat gttcttcgat cgttcctttt gtacttgtaa
     6121 acaaaacaca aatcaataca ggaatcacag aaagcaatcg tgtgaccagt cttcttagcc
     6181 aaatcgggat acgcatatgg atgaatcctt ccatgatgac ttgaccggtc aacgtaccag
     6241 taatagtaga gttctggcca gaagcaagaa gtgctacagc aaataaagtg gacaagatac
     6301 cagattttgc tacagcaatc aaaataccat tgctcatagc tgatggatca gataaagctt
     6361 catacagacc aaaaaaggaa ggatcttgga cagaaccggt tttgaagaca gcgacaccca
     6421 tgatcaatag cagtgcattg actagaaaag ccattgacaa ctggatatta gaatcccaag
     6481 tagaaaaacg tacagcttgt gctacatctt cttcgttttg gtgatcgatc ttccgagttt
     6541 gtgaaactgc tgaatgaaga tataagttat gcggcatgac tgttgcacca ataatcccta
     6601 aagctccagt cagcggagtt tcgccgccga tgaccggtga gtctgagaat gtatgactgt
     6661 tagggataaa gccagcaaat aaactgtgcc aatctgggtt agacaagcca acttgataga
     6721 caaaaacgaa taaaatgacg aaaattaagc agacaaccag tgcttcgatc ttacgaaacc
     6781 cgacttttgt cagtagtaag agaacgaaaa catctaatac agtaataaag acggcaatca
     6841 ccaatggaat atcaaaaagt aagtagaggg cgattgctgc accaatgact tctgcgatgt
     6901 ctgttgccat aatggctaat tcagtcaaga tccataaaat gacacccaat gtcttacttg
     6961 tccgtgctcg gattgcttgg gccaaatcca tttgggtcac gattcctaat tttgctgcca
     7021 tatactgtaa aagcatagca attagactag acatcaaaat aactgacatc aataaatatt
     7081 ggaaattttg tccaccagtg atcgaagtgg accagttacc tggatccata taaccaactg
     7141 ctactaatgc acctggtcca gagtaagcaa gaagtgtttt aaaaaaactt tttccctttg
     7201 gtacatccac tgttccattt acttcagcta aagataaacc atttgtatgt tcaataagtc
     7261 gatgcttatg aaattgtttg ttggattgat tattcaagaa aaaaccacct ttcctattta
     7321 tctacgatta cattctacac ctcagaaaga aaaaattaaa gaaaaagatt cggtatgcct
     7381 aatttttttt cgtggtgtgt cttgctggtt aatgaaagaa attttgttct tctctaaaaa
     7441 aaccactatt ttttgcatga aactaaagaa tacgcctttc ttttttcaat aaactttagt
     7501 aaaataaatc attgagagca attcggaaat agaaaggaga gatttatatg gtatcaacag
     7561 gtattgtcgt acttattgct attattgctt tgctaatcgg cgcagtaggt ggctttttcc
     7621 tagcgcgtaa atatatgaaa gactatttag aaaaaaatcc tcccgttaat gaggacatgt
     7681 tgcgatcaat gatgatgtca atggggcaaa aaccatctga gaagaaaatt cgccagatga
     7741 tgcagcaaat gaagaaccaa gggaaaaaat aggttctgac ccaaagactt tttgcgggct
     7801 gattttttca gtccgttttt tatttcgtaa tttgttagac tattcagact attcagcaaa
     7861 aaagacaagc acagacaatc ctatgctctt gataaagaca aaggaggagt aaaatgtcga
     7921 tttttagaaa attaggttgg ttttttcgtc aggaaaagaa acattattta attgggattt
     7981 tctccctatt cgcagtggcc ttggtacagt tagttcctcc aaaagtgatt gggatcatca
     8041 ttgatgagat cgccgataag aatttatcaa taaagatcat ttcctggtgg atcttattat
     8101 tgatccttgc agctttctta caatatcttt ttcgttatat ctggcggacg aatatttggg
     8161 gaagtgcagc tcggttggag agagaacttc ggcagcagct attcaaacat tttacagaaa
     8221 tggacaatct cttttaccaa aaatatcgga ctggagattt gatggctcat gcaacgaatg
     8281 atttgaatgc aatccagaat gtagcaggag ctggaatctt gacctttgca gattcagtga
     8341 ttacgggcgg aacaacgatc attgcgatga ttttgtttat tgattggcgc ttaacactga
     8401 ttgcgctgat tcctctgcct ttgctagccg ttacttcacg agtgctgggg tcaaaactcc
     8461 acgatgcttt tcgtgattcg caagaagctt tttctagtat caatgataag acacaagaaa
     8521 gtatcactgg gatcaaagtc attaaaacat ttggacaaga aaaagaagat ctcgcggatt
     8581 ttgaagaaaa gattgaagat gcaattatta aaaacaaacg agtcaatttt ttagactcct
     8641 tatttgatcc attcattacc ttgattatcg gtctgtctta tgttgcaaca attattttag
     8701 gtggaaagta tgtcttggaa ggcacgatct caatcgggca gatggtctca tttattagct
     8761 atatcggtat gcttgtttgg ccgatgtttg cgattggtcg attgttcaac gtattggaaa
     8821 gaggaaatgc ttcttatgat cgcgtcaatg aacttttgca tgaaaagacg catatcgtgg
     8881 aacaactggg tgcagtgcag accccggcaa aaggcgagct gaatgtagct atttcctctt
     8941 ttaactatcc gaaagaagaa tcaccagcat tagaacaaat caaatttgcg atccaagaag
     9001 gagaaacatt aggtattgtt gggaaaactg gtgcgggaaa aacgacaatc atcaaattac
     9061 tgatgcgtga atacgatgat tatcaaggga aaatccaatt tggcggacgt tcgatcaaag
     9121 aatattcatt gaatgcttta ttaggatcaa tcggttatgt gcctcaggat cacttcctgt
     9181 tttcgatgac cattcgagat aatatccgtt tttctaatcc aaaactttca gaagaagaag
     9241 tagagcatgc ggcttcttta gcatttatca acaatgaaat caaggaattg ccaaaaggtt
     9301 atgatacctt agttggagaa cgtggagtct ctttatctgg cggacagaaa cagaggattt
     9361 cgattgctcg agcattgatc aaaaatcctg aattgctgat cttagatgat gcactttctg
     9421 cagtagatgc caaaacagaa gaagctatct tgcataattt aaaagaaata agaaaagaga
     9481 aaacaaccat cattgcagct caccgtttaa gcagcgtgat gcatgcaaaa gaaatcattg
     9541 ttgtcgatca agggaaaatc attgaacgag gaacccacca cgagctgctt gcattaggtg
     9601 gctggtatga acgaatgtgg gaaaaacagc aattagaagc gaagatagaa ggaggcgagg
     9661 cctgatggat gaaaattatc aatcggaatg gacaaaatcg attccactta aagaacagaa
     9721 gaagatcatc aaacgactga tgaagttcgc caaacctttt agaagaacgt tcatcgttgc
     9781 tatcttattt gcgtttgccc tgtctgtgat caatgtccta ttgccaagga tcatccaaac
     9841 ttttatggat gatcatctgg ctaaacagtc agctacgaca caagttattt tgttttttgc
     9901 tggtgtgtac ttgtttggag tcatagtcaa aagtatcatt tggtttttcc aatggtattt
     9961 gtattccatg gcttcattga agacctatca gtatgttcgg gtaagattat ttgaaaagct
    10021 gcatacacta ggtatgcgtt attttgacca gacaccggca ggatcgaccg tttctcgagt
    10081 gacgaatgac acagaaacgc tatttgaatt ttggtatgtc tttttgatgg tattgactgg
    10141 gatctttgca gtcgtttctt ctttcgttgc catgtttcaa atcaatggag aaattgcctt
    10201 gtggactttg gtgttcttgc cgattttagg aatcgtcatc tggtattacc agaaattcag
    10261 ttctcgtatt taccgaaata tgcgggaaaa attaagtcag ctgaacacga aattgaatga
    10321 atcgatttcc ggcatgcgta ttatccagca gtttcgccaa gaagctcgtt tagctaaaga
    10381 atttgaacaa gtgaacaacg agtatttaga cacgcgctat gcgatgatca aaacgaattc
    10441 gattctgtta agtccaatca tcaattttct ctatgcattg gcgattgcgt tggcgttgac
    10501 tttgtttggg atcgatgcgt tgcattctcc cgtagaagtt ggactgatct atgcatttac
    10561 gacctatgtc caggaatttt ttaatccaat gtctcaaatg atggactttt tgagcacctt
    10621 tacagatggg atggttgccg gtagtcgtat tttgaaaatc atggatacag aagaatatgc
    10681 accgcaacaa aatcctggag cagatcaagc gattacttca ggtaagatcg agtttcgcaa
    10741 tgtaagcttt tcttatgatg gagagcacaa tgtattgaac aatatctctt ttgtcgctaa
    10801 tccgggtgag accgttgcat tagtggggca caccggaagc ggaaaaagct cgatcatcaa
    10861 cgtattgatg cggttttacg aattttatga aggccaaatc ttgattgatg atcatgacat
    10921 tcgagaatat ccaatggaag agttacgtaa gaaaatggga ttggtcttgc aagatgcctt
    10981 cttgttttat ggagatattg caggaaatat ccggatgatg aatccagcta tcacagacga
    11041 acagattcgc catgcagctg agtttgttca agcaaacaag ttcatcgatg aattaccaaa
    11101 aggatatcac tcaaaagtga tcgaacacgg tgccagctat tctagtggac aacgacagct
    11161 gatcactttt gcgcgaacaa tcgtgacaga tcctaaaata ctagtattgg atgaagcaac
    11221 agcaaatatc gatactgaaa ccgaaggatt gatccaagaa ggattggcaa aaatgcgaca
    11281 aggacggaca acgatagcga ttgcccatcg gctgtccacg attcgggatg cagaattgat
    11341 tttagtttta gacaaaggtc atatagtaga gcgtgggaat catgaaacac ttctagcgca
    11401 aaatggtttg tatgcagata tgtacaaact gcaaagcagt ggtgtgtaaa aagaggttgt
    11461 gacagtagca tagtagcatt ttgccctaag cgctagagtg aaaataaaac agagcttcgc
    11521 agattcttca tctgtgagcg ttagtggcgt agtcacccga tttaaaaaag acgacgacag
    11581 ctagtcaaaa gcggctaata accactaaga aagcgttgac aaaagcgttt gctttcgata
    11641 atataaagaa aacgatacaa cttggatagt gattgcctga tgcaagctaa acctgatgag
    11701 tctcccatgg ttccatggac ggctttttgg gtttttcttt ttgttttgga tacagcccta
    11761 tatatcatgg aaaggagcaa aacatgctga ttgagaaagt actgaataac aacgtcgtaa
    11821 taaccaggga tgaaagaaat caggaaatga tcgtgatggg aaaaggtttg gcattcaaaa
    11881 agaaagtggg tgacccgatt tttgactcat gcatcgacaa aaaatttcag ttgacaaatg
    11941 acgggctttc acaacagttc caagaattgg cacaagcgat tcctcttgag tacttagaaa
    12001 tttcttgtga aatcattgaa tatacaaaag aggctctaca agtccagttg aatgattctg
    12061 tgtatattgc attgacggat catttgtata cggctatcga acgtcacaaa atgggaatat
    12121 ttgtaccaaa tgtattgctg tatgatatca aacaactatt tcctaaagag tatgccatcg
    12181 ggaaaaaaaa cagtgaagaa aatctcggat aaataccaca tcaagttatc agaagatgaa
    12241 gccggattta tcgcattgca tttagtcaat gcacaaggca ttcaaagcca acaagacaat
    12301 acctacctgt tcactataac aatacaggat attctaaata tcgtccgcta ttactataag
    12361 attacatttg atgaagattc agtccatttt tttcgattcg tcacacactt acgttttttt
    12421 ctgacacgcg tcctagatgg agaaaaatca gaagtgggag aagtttctga ggaattgttt
    12481 caggttattc aaaaaaaata taaggatacg tttggttgcg ttgagaaaat agcagacttt
    12541 ttagccgaga cgttcaacta ccagatgtcc acggacgaaa aaatgtattt aacgatccat
    12601 actgcgcgtt tagtgcatga gaatcgttgt aaataaataa aaaaaggagg ataaattaac
    12661 aagaacgtat gaaataaggg tagtgacatt cagttggcta aaccttccac taataaattg
    12721 gagggaatag aaatgggaaa ataccaagaa ttagcgagaa aaatcgtaga aaatgtcggc
    12781 gggaaggaaa atatcaatgg tttgacacat tgtatcacaa ggcttcgttt caaattaaaa
    12841 aacgaagaaa aagcaaatga tgagatcttg aaaaatatgg acggtattgt aacggtaatg
    12901 cgagcgggag ggcagtatca agttgtcatc gggaatcatg tgccagtcgt gtttgaagag
    12961 gttataaaag caggaaacct tacttttgat gaggctgttt caacagaaaa aatgagaccg
    13021 tttgatatgc ttattgatgg aatatcgggc tgtttccaac cattccttgc tatcctagct
    13081 gctgacggga tgattcgtgg actgacagca ttccttgtct tcttaggcgc gtttgaccgc
    13141 ggttctggca catttgttat gtttgataat atcggtgatt ctgtattcca atttatgcct
    13201 gttattatcg gtttaacagc tgcccgtaaa tttaaagtaa atgaattcgt agggatgttg
    13261 cttggcgcag cattaatgaa tcctagcttg tcgctggaag ctttgagtgg tgcagcagaa
    13321 gcaccactaa cgaccatatt ttctggcact atttttgaag cacctattta tcaaacagta
    13381 ttcggcattc cctggattgc gcgtaactac gcttcttctg tcattcccat tatctttatt
    13441 gtgttgttag ctagccaaat tcagaaacca attaaaaagt tagtacctga gatgatcgcg
    13501 aatttcttcg ttccattctt caccgttctt attacaatgc ctttaggttt tttactcgtt
    13561 ggaccagtat tcacattcgc aactgatatt ttaatggctg gatttgaaac acttttagca
    13621 ctttcgccag ttatttatgg agcaatcgta ggcttcttct ggcaaattct ggtgatgttt
    13681 ggtctccatt gggcaatcgt accaatggga ttgatgcaat tttctgtgaa tggttggcaa
    13741 aatattatga cacctgttgc agtagtgtct tttggacaaa ctgcagcact taccgcactc
    13801 tactttaagc ttagaaatcc aaaagacaaa gcaattgcaa taccagccat tgtttctggt
    13861 attgtgggga ttacagagcc agctatttat gggtttacat tgccacgtaa aaaaatattt
    13921 atttttacct gtgtcggtgg tgcgttaggg ggcgcgtata gtgggttaat gaatttaact
    13981 tcttggaatc aaggtggact aggcatcttt acaattccca actatattag accagatggt
    14041 gatttaacag atgtgattaa tgtattgatt gggatcgcca ttgcgatggt ggtatccttt
    14101 gcgctgactt ttttcttttg gaaggaggaa gcagatgaaa cagatgatag acagaaaaga
    14161 tcaggtaaag aaattgtaaa aacaccgata caaggacaaa tcgctccact gaatgcagca
    14221 aaggatgcag cttttgcaca aggaacacta ggtcgaggaa tcttgatcta ccctgaaaaa
    14281 ggagaagtac gtgccccttt tgacggaaca gtcatgtccc tgtttccgac gaaacatgca
    14341 atcgggatgg tttctgaagc aggtctggaa ttgctgattc atgtcggatt ggatactgtt
    14401 cagttggaag ggaaatattt tgaaagcttg gtgcagcaag gagaaagagt gaaacaaggc
    14461 gatctgttgc ttcgcttcga caaaaagaaa atcgaagagg aaggatacag tttagaaaca
    14521 cctgtgattg tcatcaatta catggattat ttggatattt tggaaaatca aggggaaacg
    14581 gtcgggccag acgatgcact tatcaccgca ctgacctgaa cggttaggtg aaagaatgga
    14641 ctaggcttaa ctattctttt tagtccactt ctttattttc aaaattgaaa ggctctttca
    14701 tgatggctga gctgataggg cgtttatcaa atttttgtcc tacagaaatt agatggacta
    14761 aattctgttg ataattttct gcaaaaacac aactatataa cacgtctaga agataacaaa
    14821 tcgatgtatt gatcgtaaaa ttaccaattt tcgaatacag tcgttcgcgt gtcgtaagtc
    14881 gcaaagcggc tgtactcatt gaagatagcg ttgaatcacc gatactggtc aaagctaaaa
    14941 ttggcacatg attttgtttc aatatttctg cggtacgaag gatcacattg ttttcacccg
    15001 tataagaaat cagaatccca cacatatcac ttgtgcaatt gtacgcttca tagaacgatt
    15061 caccaaaagt ggtgacaatc gtgacatcca ttttgatgcg cttcattttc agcatgaaat
    15121 cttgagaaat caaagtattc gagttactgg cgaaaatcac gatttttttt gcttgaagca
    15181 ttagttgttt ggctttttgc agttggtcat gatgaatgag agaaagtgta tcttgaatcg
    15241 tcatctgttc taaagaagcc attttatttg cgatcgtcat aataccgtct gtggtggaaa
    15301 aaggaagatt cgcatctacg tcttgaaaat tcccttcaag ataggctgtt tcagctaaaa
    15361 aagcattacg aaaactgacc cagccatcaa aacccatttt ttttgctaca cggatgagtg
    15421 tggacgggtg gacataacat cgctcagcaa tctcttggat ggtcatttcg ccaagtgctg
    15481 gagaatcgct taagagaaac tgaatcactt cattttctga aggagaaaag gagatttctt
    15541 tgattttttc acgtaataac atgtagatac cttcttcttg tttttttacg taaatcataa
    15601 cttttttgaa aatgatttgt aaacggattt atgttatata gcttaataaa gacatttttt
    15661 gacaagatat ttacaaaaat ccagaagaaa acactaactt ttgtaaatgt tactggaaag
    15721 aaacgaattt ctattgtttg ttttgtttac aaaaaagata ataaaagtgt aaaaagtaaa
    15781 ggagtgagaa catggcaaaa ggaatcaaaa tcgctacgat tggtggaggc agcagttaca
    15841 cccccgagtt gatggaagga tttatcaaaa gatatgagga attaccgata cgagaaatct
    15901 ggctcgtaga tgtagaagaa ggaaaagaaa agcttgagat cgtcagtcag atgtctcagc
    15961 gtatgtggga tgcttctccg tatgaagtca aaatctatgc aactctggat cgggaagaag
    16021 cattgaaaga tgcggatttt gttacgactc agtttcgtgt cggtcaatta gatgcacgaa
    16081 tcaaagacga gcggatccct agttattatg ggatgattgg gcaagaaaca aatggtgctg
    16141 gcgggatatt caaagctttt cgtaccgttc cagtggtttt ggcaatcgtt gaagacatga
    16201 agcgtttatg tccaaatgct tggctgatta atttcgcgaa cccaagcggg atggtgacag
    16261 aagcagttat cagatatggc aaatgggata aagtgatcgg attatgcaat gtgccagtaa
    16321 tggcgatgat gacagaacca acaatgcttg gcaagaaaaa agaagagctg atctacaagt
    16381 ttgccggtct aaatcacttc cattggcaca aagtagcaga taataaagga aatgaccgca
    16441 cggcagaatt gattgatcgt ttatacgatg aagacaatgg attgccgaaa aatatctttg
    16501 acatcccgtt tttcaaagaa caattaaaac aaatgaacat gatcccttgt ggctaccacc
    16561 gctattacta tcgtgaagaa gaaatgctgg aacatgcttt agaagaatat caaacgattg
    16621 gtacaagagc gcagcaagtg aaagaaacag aagcagagct gtttgaatta tacaaagatc
    16681 cagaattgga tcataaacct gaacaattgc aaaaacgagg tggtgcttat tattcagatg
    16741 ctgcctgtga aacgattgct tcgatctatg gaaataaaga aacacagatc gtggtatcta
    16801 caaagaatga tggagctgtt cctgatctgc cggcagattg tgtagtagaa gtcactgcct
    16861 atgttggcgc acaaggcgca cgaaatgtag cgtttggttc ccttcctgct gctgaaagag
    16921 gctggttgca agtaatgaag aatatggaat tgctgacgat cgaagcagcc gtgactggtg
    16981 attatggaac agctttgcaa gcgtttacga tcaatccgct tatacgatca ggaaagacag
    17041 caaaacggat catggatgag ttgtttattg cacatcagca gtatttaccg caattcaagg
    17101 aaacaatcag ccggttaaaa gcagaaggaa tcgaagtcca agatgaactg gcaagagaat
    17161 tatcaagcaa tacacatgta atggaggaag tatgatggga ttcagaaaag attttttatg
    17221 gggcggagct acagcagcca atcaatgcga gggcggttat gatgaaggcg gacgtgggtt
    17281 agcgaacgta gatgttgtgc caatcggaga agaccgtttt cctgtcatta ctggaaaaaa
    17341 gaaaatgttt gaatttgatg aaaatcattt ttatccagca aaaattgcga tcgatatgta
    17401 tcatcattat aaggaagata ttgctttatt tgcagaaatg gggttcaaga cttatcggat
    17461 gtccattgct tggagtcgta tttttccaat gggcgatgaa aaagagccaa atgaatctgg
    17521 gttgaaattt tatgaggatg tctttaaaga atgtcaaaag tacgggatcg agccgcttgt
    17581 gacgattaca cattttgatt gtccgatgca tttgatcaaa gaatatggtg cgtggcgttc
    17641 cagagaactc gttggttttt atgaaaatct gtgccgagtc atcttcaatc gctataaagg
    17701 acttgtcaaa tattggttga cattcaatga gatcaatatg atccttcatg caccattcat
    17761 gggcgcaggg ctgtattttg aagaaggaga aaatgaagaa caaatcaaat atcaagcggc
    17821 acatcatgag ttggttgcaa gtgccatcgc aacgaagatc gctcatgaag tagatccgga
    17881 gaacaaagta ggctgtatgc tggcagcagg agcatattat ccatacactt gcaatcctga
    17941 agatgtttgg gcttctagaa agtcagatcg agaaaattat ttcttcattg atgtccagtc
    18001 acgaggagaa tatcccgctt atgcattgaa agaaatggaa cgaaatggca ttcagatcaa
    18061 aatggaacct ggggatgccg cattgctgaa agaacatact gttgatttta tctccttttc
    18121 ttattattct tctcgagttt cgactactga caacgaactc ttggaacaga ctgcaggaaa
    18181 tatctttgct tctgtcaaaa atccatatct ggaagcaagt gaatggggtt ggcaaattga
    18241 tccattaggc ttgagaataa cgatgaatga tatttatgat cgttatcaaa aaccactatt
    18301 tattgtagaa aacggcttag gtgcagtcga tacgcccgat gagaatggtt atgtggaaga
    18361 tgattatcga atcgactatc tggcagcaca tatccaggca atgaaagatg cagtagaatt
    18421 agatggagtc gatctattag gctatacgac ttggggatgt attgatttag tatcagctgg
    18481 tacaggtgag atgaagaaaa ggtacggatt catttatgtc gatcgtgaca acgaaggaaa
    18541 tggcacatta gcccgttcga agaagaaatc attcaactgg tataaaaaag taattgattc
    18601 aaatggagaa aatctttcga acgattgatc aatgatttat aaaagtctat tgtttttcag
    18661 cggatttttc gagtataata aagctattcg agaaacgcaa gcagaacaat agactttttt
    18721 tcgcattggc tgtaaagtat gcttacttga ttaagtaaag gagaatctac atatgtatga
    18781 tgtaatcgtt attggcgcag ggcctgctgg gatgactgct gctttatatg cttcacgttc
    18841 aaacttatcc gtattgatga tcgaacaagg cgctcctggc ggccaaatga acaatacggc
    18901 agaaatcgaa aactatcctg gtttcgattc aatcatgggt cctgatctag cttataagat
    18961 gtatgaaggt gtttcaagat ttgggacaga aaatgcttat ggaatcgtgc aagatatcaa
    19021 ggatcacggc agccacaaag aagttatttg tgaagataaa acttacgaag gaaaaacagt
    19081 cattatcgca acaggctgta tccaccgtaa attaggcgta cctggcgagg aagaatttgc
    19141 tggacgtggc gtttcttact gtgcagtttg tgatggtgca tttttccgta acaaacgtct
    19201 agtagtgatc ggaggaggag actcagcagt agaagaagcc atctatttga cacaatttgc
    19261 ttctgaagta gtcatcgtcc accgtagaga tgaattacgt gcacaaaaga tcatccaaga
    19321 ccgtgcattt gcgaatgaaa aaatttcctt tatgtgggat acagtcgttg acgaaatcgt
    19381 tggaaatgaa atggtagtga caggtgtgaa agcacgtaat gtcaaaactg gcaatgagtt
    19441 tgatttggca gcagacggag tattcatcta tgtaggattg gatccattaa ctgcaccatt
    19501 tagagaagct gggttgacga acgaagccgg ctggattcca acagacaacg aaatgaagac
    19561 gaatatacca ggcgtttttg ccatcggaga tgttcgtgaa aaagatttgc gacaaatcac
    19621 tactgctgtt ggtgatggtg ggattgctgg acaacaagtg tataaatact tggaatcttt
    19681 agaagaaaca actgcataag tttaaaaaga gtgaaagaac ggtttcaatt gttcttttac
    19741 tctttttttt gcgtgaaatc tactgaatcc gatttatgcg aatacttgta accgtgcttg
    19801 ataaaagaat cttttcttga tttaagcgtt ttttggtcct catactttca tatctaataa
    19861 gtgaatgcta tacttattaa gaaccatatt tcagaaagag gtgttatttt catgtcttgg
    19921 gaacaagtgt atgaacaatg ggtacatgaa gaaaatttag aagaaaactt aaaaaaacaa
    19981 ttaaatgatc tgggtgaaga tcctgaaaaa cttgaagatg ctttttatgc accgttggaa
    20041 ttcggtactg caggcatgcg gggaatctta ggacctggga tcaatcgaat gaacatctat
    20101 acggtaagac aagcaacaga aggacttgca cgtttcatgg atacacaagg tgaagaaaca
    20161 aaacgccgag gtgtcgctat cgcttatgac tcacgccatc aatcaccaga atttgccatg
    20221 gaagcagcca aaacattagc acatcacaat atcccttctt atgtattcga aagtttacgt
    20281 ccaacaccag agctttcttt tgcagtccgt catttgaaga cgtttgcggg aatcatggta
    20341 actgcatccc acaatccagc agcttacaac ggatataaag tctacggaga agatggggga
    20401 caaatgcctc ctgcagacgc agatgcattg acgaaatatg tccgtgaagt cagcaatccg
    20461 ttaaaagttg aagtattatc tgacgacgaa gctgaacata gcggattgat cacgatcatc
    20521 ggagaagaag tggatgccgc ttatctagaa gaaatcaaag cagtaacgat tgatcatgaa
    20581 ttggtggaaa caatgggtaa agatttgaag cttgtgtaca caccacttca cggaacagga
    20641 aaaatgttgg gcgaacgtgc gttgaaacaa gctggattcg aacaattcgt actagttcca
    20701 gaacaagctg tcgcagaccc ggatttcact acagtcaaat caccgaatcc agaagaacac
    20761 tctgctttcg agtatgcgat gcgacttggc gagaaagaag gcgctgactt actgattgca
    20821 acggatcctg atgcagaccg tttaggcgca gccgttcgtt tgccaaatgg ctcctatgaa
    20881 gtattgacag gaaaccaaat cggagccctg tttgctcaat atattttgga agcacaccaa
    20941 tcgcgaggca ctttacctga aaatgcagcg gtattgaaat caatcgtttc tagcgaattg
    21001 cctacagcaa ttgcggaaca ctatggggct accatgttca atgtattgac aggattcaaa
    21061 ttcattgctg aaaaaattca acaatttgaa gaagatcatt cccatacttt tatgttcgga
    21121 ttcgaagaaa gttacggtta tttagttaaa cctttcgttc gcgacaaaga cgcgattcaa
    21181 gcattgctct tattagcaga agtagcagct tactataaaa aacaaggcaa aacattgtat
    21241 gacggcttgc aaacgatttt tgaaacatat ggctactacg cagaaaaaac gatttctgtg
    21301 acaatgagcg gacaagaagg ctctgcaaaa atctctgctt tgatggaaaa attccgtgaa
    21361 gaagcaccga cagaatttgc cggagcaaaa gtcatccaaa cagaagactt caaagagttg
    21421 acgagaacag accgtgaagg aaacgtagaa aaactaacga caccgccatc tgacgtattg
    21481 aagtatacgc tggaagaaaa tggctggatc gctatccgtc cttcaggaac tgagccgaaa
    21541 atcaaatttt atatcggtgt aaaaggaaac tcttcagaag acgcagctga aaaagtagcc
    21601 gcttatgaag cgattatcaa aaaaatgaca gaagaataaa aaaacttgag actgatggaa
    21661 aaagtggtaa actttagaag tcagtctttt ttcaagttgg aggtgaatgg attgccagac
    21721 acgcaaaaaa caaaagtaga gaaagtcagc caactattca aaatgttgag tgaccccact
    21781 cgtctcaaaa ttctgctgta tctgaaagac ggggaacaaa atgtgacagc aatcacccaa
    21841 gcagtagaaa tggaacaatc tgctgtatcg catcaactgc gtttattaag agaaaaccat
    21901 gtcgtcaagt cccatcgtga gggaaaagcg attttgtata gtcttgatga ttctcacgta
    21961 ctggatattt tgaaccagac gctcaaacat gtggaacatc aataacgata ggtgcaaata
    22021 gagacaaact ggactgtatc aacgaatctt cttcgctgac acagtccagt ttttttaaga
    22081 aaaagacaag aaaaactgtt agtctgtttc ttcggtggtt tcagttccat aataactatg
    22141 atcttctagg tctaaagcat tcaaaataag tgaagctgtt tcttcgatag ataaagaagc
    22201 gacattgatt actacgcagc ccaatttttc ataaagatca ttcgcaaaat cgagttcagc
    22261 tcgtattttt tcgatatcgg aataagcagt atcaggattt aatccgtaag agcgcatccg
    22321 ttctttacgg atattgttta aaatatctgg gttattcgtc agtcccacga tttttttagg
    22381 atcgatctcc caaagctgtt tgggaatatg tgcttgtggg atcaaaggta gattggcaac
    22441 ctttaaattt ttgttagcta aaaacaagct caaaggcgtt ttggaagttc tggatacgcc
    22501 tagcaaaacg acatctgctt ctagaaaccc acgaggatct ttcccgtcat cgtattttac
    22561 agcaaattcc attgctttga tccgtttgaa atagttctca ttcaataaat gaagggcgcc
    22621 aggttgccga atgggcttga taccagttaa gcgctcgatt tctgatatcg gcggagtcat
    22681 cacatcaaaa ctaaataatt gatgttcttc acaaaaagaa cgcgtcatct cgatcaattc
    22741 ttgattgatc aatgtgtgta ggacaagggc tttttctttt agtgcatctt gcagtgcctg
    22801 catcaatgtt ttctcatctg tcacaaatgt tctgcggaat aaattgaact caacggtagg
    22861 atattgggcc atagtggctt gcgctaattt tgtcgcagtt tctccagcag agtcagagat
    22921 gacaaaaaaa gtaatgattt ctgtagaatc ggaagacatg gataaaacct cacttgcatt
    22981 ttttttatgt ttattatacc atactctaga atgtgtactt cgtaatcatt ttgatttgga
    23041 ggaataagtc atgaaaaaac agacattgat tgaacaatca ctagaaacat tgaaaaataa
    23101 cggttttaaa tatactaaaa aaagagaagc attgttaact tatttagtga agcgcaatcg
    23161 ctacgtatcc gctaaagaag tttttgacta tatgaatcag gaattcaaag gagtaagtta
    23221 cgatacgatt tatcgaaatc tccatgattt tgcagaaatc gatttactgg aagaaaccga
    23281 tttaaatgga gagatgaagt ttcgttttca ttgctgtcaa ggggaaatcg gccatcacca
    23341 tcactttatc tgtacggtgt gcggaacaac gaaagaaatc catatgtgtc caatgaatta
    23401 tttcgaagaa cagctcggtg gatgtacgat cgaagggcat agattcgaga ttttcggaag
    23461 atgtgaaaat tgtcaataag gatttaaata aaataacatt tgataatttt aaaaaaaagc
    23521 aagatttcct tgacggtcaa aacagcatta gatataatat ttacaggaca gtcttgtttt
    23581 ttgagatata tttcaataac aattacggtt actttttaag ctcggaggga gggaattaac
    23641 atgtcaaaaa cagtcgttcg caaaaacgaa tcacttgacg atgctcttcg tcgcttcaaa
    23701 cgttccgtgt caaaagcggg tactttacaa gaatctcgta aacgtgaatt ctacgaaaaa
    23761 ccaagtgtaa gacgcaagaa aaaatctgaa gcagctagaa aacgcaaaaa attctagttc
    23821 tgtgatgaat tggagttggt agtgtgtcac tactaagtag attaaacgat gacatgaaaa
    23881 cagcgatgaa agcgaaagat aaggaatctt tacaagtcat ccgaatgatc aagtcatcta
    23941 tacaaaacga gcagattaaa gaaggtcacg acttgacaga agaagaagaa ttaacggtgt
    24001 tgtctcgcga aatgaaacag cgacgtgatt ctttgcatga gtttgaagaa gcaggacgtg
    24061 acgatttagc tgaaaaagta aaaagtgaaa ttgtgatagt cgaaaaatac atgccggaac
    24121 aactttccga cgaagaaatt cgacaacttg ttcaagaagc aatcacacaa actggtgctt
    24181 catcaatgaa agaatttggc aaagtcatgg gtgcgatcat gcctaaagtc aaaggaaaag
    24241 cagatggaaa ccaagtaaat gccattgtga aagaactatt acaatcttaa ttttatcatt
    24301 taaaggctct tcgtcaatga gtgttggtga agaaattcag gctctttgtc aaatgatgtt
    24361 ggtaaagaga aatcttagaa taatttggac agaacagaaa gaagaaaacc tctttctgtt
    24421 ctgtttattt ggttaaaaat gttttgc
//
LOCUS       DAZKLA010000030        24311 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000030 DAZKLA010000000
VERSION     DAZKLA010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.030"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(92..826)
                     /locus_tag="ACG85N_001036"
     CDS             complement(92..826)
                     /locus_tag="ACG85N_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0223823.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1153..1563
                     /locus_tag="ACG85N_001037"
     CDS             1153..1563
                     /locus_tag="ACG85N_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFQ0223824.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1556..2257
                     /locus_tag="ACG85N_001038"
     CDS             1556..2257
                     /locus_tag="ACG85N_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFQ0223825.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2257..3843
                     /locus_tag="ACG85N_001039"
     CDS             2257..3843
                     /locus_tag="ACG85N_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0223826.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3862..4083
                     /locus_tag="ACG85N_001040"
     CDS             3862..4083
                     /locus_tag="ACG85N_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFQ0223827.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4080..4841
                     /locus_tag="ACG85N_001041"
     CDS             4080..4841
                     /locus_tag="ACG85N_001041"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFQ0223828.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5221..5730
                     /locus_tag="ACG85N_001042"
     CDS             5221..5730
                     /locus_tag="ACG85N_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0223829.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5805..6344)
                     /locus_tag="ACG85N_001043"
     CDS             complement(5805..6344)
                     /locus_tag="ACG85N_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFQ0223830.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6484..7095)
                     /gene="rpsD"
                     /locus_tag="ACG85N_001044"
     CDS             complement(6484..7095)
                     /gene="rpsD"
                     /locus_tag="ACG85N_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFQ0223831.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7357..8208
                     /locus_tag="ACG85N_001045"
     CDS             7357..8208
                     /locus_tag="ACG85N_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFQ0223832.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8236..8871
                     /locus_tag="ACG85N_001046"
     CDS             8236..8871
                     /locus_tag="ACG85N_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFQ0223833.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8891..9664
                     /locus_tag="ACG85N_001047"
     CDS             8891..9664
                     /locus_tag="ACG85N_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0223834.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10072..10869
                     /locus_tag="ACG85N_001048"
     CDS             10072..10869
                     /locus_tag="ACG85N_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFQ0223835.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10847..11260
                     /locus_tag="ACG85N_001049"
     CDS             10847..11260
                     /locus_tag="ACG85N_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0223836.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11244..13889
                     /gene="mutS"
                     /locus_tag="ACG85N_001050"
     CDS             11244..13889
                     /gene="mutS"
                     /locus_tag="ACG85N_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0223837.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13907..16015
                     /gene="mutL"
                     /locus_tag="ACG85N_001051"
     CDS             13907..16015
                     /gene="mutL"
                     /locus_tag="ACG85N_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFQ0223838.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16037..16597
                     /locus_tag="ACG85N_001052"
     CDS             16037..16597
                     /locus_tag="ACG85N_001052"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFQ0223839.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16682..18670)
                     /gene="treP"
                     /locus_tag="ACG85N_001053"
     CDS             complement(16682..18670)
                     /gene="treP"
                     /locus_tag="ACG85N_001053"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFQ0223840.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18893..19606
                     /gene="treR"
                     /locus_tag="ACG85N_001054"
     CDS             18893..19606
                     /gene="treR"
                     /locus_tag="ACG85N_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFQ0223841.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19683..20129
                     /gene="msrB"
                     /locus_tag="ACG85N_001055"
     CDS             19683..20129
                     /gene="msrB"
                     /locus_tag="ACG85N_001055"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFQ0223842.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20299..20901
                     /gene="ruvA"
                     /locus_tag="ACG85N_001056"
     CDS             20299..20901
                     /gene="ruvA"
                     /locus_tag="ACG85N_001056"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFQ0223843.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20914..21915
                     /gene="ruvB"
                     /locus_tag="ACG85N_001057"
     CDS             20914..21915
                     /gene="ruvB"
                     /locus_tag="ACG85N_001057"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFQ0223844.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21944..22522
                     /locus_tag="ACG85N_001058"
     CDS             21944..22522
                     /locus_tag="ACG85N_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0223845.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22574..23056
                     /locus_tag="ACG85N_001059"
     CDS             22574..23056
                     /locus_tag="ACG85N_001059"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFQ0223846.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23173..23547
                     /locus_tag="ACG85N_001060"
     CDS             23173..23547
                     /locus_tag="ACG85N_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223847.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(23922..>24311)
                     /locus_tag="ACG85N_001061"
     CDS             complement(23922..>24311)
                     /locus_tag="ACG85N_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0223848.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT         7916 a         4359 c         5203 g         6833 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgaaata aaaaaacttg gtacgcaatc
       61 gctgtaccaa gtttttttat ttagcttttg attaagccat aaataataat ttatataaga
      121 atactgctac gacaccagca cagataggtg ctacaactgg tacccatgca tatccccatt
      181 gagaagaacc tttatgtttc aatggaagaa gtgcgtgtac aagacgtgga gcaaagtcgc
      241 gggctgggtt tagtccagga cctgtaggac caccaagtga agcaactaat gtccatacaa
      301 ggaaaccaag tgctaagtga gctgtaccaa gattatcttg gaagaatggt gcttttgtaa
      361 tacctagggc accaaagaat aaaacgaatg aaccaaagaa ttcattgacg aaaccattga
      421 atttagaacc aacgctgttg attgttgaga atgtacctaa cacatggttt acgtcttctg
      481 tttggtcgta gtaaggtttg taacaagcta ctacgatcaa ttgtccaatc gctgcaccaa
      541 tcaattgtgc aatgatataa ggaacaactt cagcccaagg gaatagacca ttagatgcta
      601 aagcaattgt aaatgctggg ttgatgtggt taccgctgat tccaccaaaa atcattgctg
      661 gcatcataac cccgaatcca tacccgacag caatcagcat ccagtcactg ccatatcctt
      721 ttgtaccttt taaatctacg tttgctacag caccgttacc tagaacgatt agtaatgctg
      781 taccaatcat ttccgccgca agacgggtca ttagtgagta atccataaat acctccaaaa
      841 gttataaaaa tgcaaaaaaa aaagaaacat caacacgaaa agcatcggtg cagtgtttcg
      901 tgtcggaagg atttctgcag tcttaacaaa tgtgaataaa aaaatgaatt ccattactta
      961 taagaaacca gaccaacagt aaaactccaa ctctttttaa aagttctcca caaatcacca
     1021 agcttaacta taaatatacc aaaaaaaaaa cgtttttttt ctcaaaaatt gtatcttaga
     1081 aacagcatgt tttgaaactt tcagaaaagg tttgttagta aaatgaaagc tatacgtaag
     1141 gagtgttgtt tgatgaaatt ttttcaagag tttttcatta ttattttctt ttctgtatta
     1201 ggagaaggca tacggatttt tgctcattta cctattcctg gaagtatctt aggtattctt
     1261 tttttatttt tagcatttga aatgcatctt gttgatcctg aaaaaatcgg agcaactggt
     1321 gattttttat tgaataatct agccatttta tttgttcctg ctggtgtagg tttattggaa
     1381 tattttgatg atattgcttc gatatggcct gttttactgg gagcagtcgt cgtgtgttcc
     1441 atcgtcacga tggttgcagc gggaaaaacg gcagaaggtg tagaagcttt gctgggctat
     1501 gtccgtaaga aaaaagcaga aagggtatct gtaaacgaaa tggaggagaa tcaaattgac
     1561 tgagtttttt gtgaatccat tatttggctt aagtttgact ttaggagtgt acgtattatc
     1621 tgacaaattt ttgcggaaat ataaactgcc tgttttgaat cctctcgtat tatcgattgt
     1681 tgttattatc ttatttttgc atttttctgg gatcagctat aaagactata atatcggggg
     1741 caattttttg actatgctga ttactcctgc gacagtggca cttgccatcc ctctttataa
     1801 aaacttccgt ttactgaaag agaattttta tcctgtaatg gctgcgattt tagcaggaat
     1861 agtcgcaaat tgcttggtta gtgtagggtt cggttacttc ttctctcttc ataaagagat
     1921 ggtgatctca ttactaccta aatctgtaac gacggctatt tctgtcgatt tatctcatac
     1981 gatgggcggg gtgaatgccg tgacacttgc tattgttgtc tctacaggta tttttggttc
     2041 gctgatcgcc actcatattt tccgcctatt caagatcgaa agtccagttg ctcgcggtgt
     2101 ggcgttagga agtacttctc atgctattgg tacagcaaaa gcaatcgaga ttggtgaaat
     2161 agaaggaatc atttccggac ttgcgatatg cgtcaacgga atattgaccg ttttattgct
     2221 accgttattc tttcagcctt ttgctggctt gttttgatgc gaaaaccact attttatgct
     2281 acaataacca aaacgttcga gcataaaaaa ggagggaaag acgtgtattt gtcttccgag
     2341 tatcttaaac aatttgataa agatctatat tataagattc tcctcctaga aagttttgag
     2401 gatcaagctt ggcataccgc tgcacaatta gcgcaagttg ttcagctgga tgcccgcagc
     2461 gtgtcgaaat acctgaacga gctgtctaaa aactatcagc agtttagcgg taagacacac
     2521 cctttgttta caaaaaatca ccgttctggt tataactttt atgatacgct cgattcaatt
     2581 gaacatgagc gttttttgat ctatctcgtg caaagtacgc tgaaattcca gttgctgcac
     2641 gacatttttt ttgaagaatt ccatacaatg taccaatttg cgcaaaagca ttacattagt
     2701 gaatctactg ctcatcgaaa aatcaatgaa tggaaacagc aactgcaaac ttacggaatt
     2761 cggctgcaac gaggtacata tatcgctcaa ggcgaagaag aaatcattcg actttacttg
     2821 catatgacat tctggcagtt gtttcgaggg aaaatctggc catttgaaac gatttctcaa
     2881 atggacgtaa agaatatggc agaacatatc atggcttttt ttaatgttcg tctgaacgag
     2941 atcaaaaaaa gacgattgga atatatgcta ggtgcctttt ttttaagaaa atcacagaag
     3001 cattatgttg tattgaatga gaaaaaaaga cggctgattt ctgacaatct gttgttccag
     3061 cgattttgcc aggtcatgga gccggtattt cctaattatt ttcaagtaga agacgagtta
     3121 ggcgcattat ttcttgtact gatgactcgt gaagaatatt atagtgatcc taagatacgg
     3181 aagaaaattt ttgattttca tcaacaagca aaaacaccgc cctttacagc cctttcagaa
     3241 gcaaaagccg ctctttctct ttatcaggaa gagcaggggc tgccagcaga aaatctaacc
     3301 ttcgaggcag aaaactattt attcagcagt cattttttcg cctatctttt tcctaatgcg
     3361 aaagaaacga ttgatggcaa tagtagtgat tttattaatc atttagttat tgaaaacaag
     3421 gaattgaaac aatggctcgt acattttttt gaaagtagac acaaacatcc caatcatctg
     3481 gcatttaaaa atcatgcgtt tttgatgggg cggtatctga ctgtgtttaa aacattagga
     3541 gcctttactc cacagcttcc aaagatcacg atccttttaa tgacagattt cccattattt
     3601 gaagagcaac tgttagagga gggattacgg acatttttcc gtaatgagta tcagctgatt
     3661 tttctgccaa cagattatcg ggggagagaa gttgatcttc tgatttcgac aagtaaagtc
     3721 caccgcaaac catgggcgga tcttgactat tttattgtga ctgaagaact aaaattaatt
     3781 gattacatcc aactatctca aaaatttgaa atgattcaaa aacaaaaact attaaaacaa
     3841 taacaaagaa ggagaaaaaa gatgacattt gaagaaattt taccagaatt gaaaaaaggt
     3901 gcaaagatta ttcgtaaagg atggagtgga tttgaacttt atgttgttct cgtatccgat
     3961 gatgaatttg atggctgtcc ggtgacgccg tacttattga tcaaaacaag tgacgaaggt
     4021 ttttccagct tcgctcctac agtttgtgat attttagcag atgattgggc gatcgtagaa
     4081 tgaaaaacca gtatccagat ctagctaaac aggttgtatt tatcacagga gctgcttctg
     4141 gaattggtgc tgcgcaagcc cgagcgtttc ttgaacaaga tgcttttgta tttggattag
     4201 atcaaaagtt cggtaaaatg gaagaaattt ctttgcgatt tcctgaaact tttgcctacg
     4261 cactaggtga tgttcgacaa atgaaagaac tccaacaagc ggtatcgaaa tgccaaaatc
     4321 attttggtga agttacgatt ttactgaata cagcggggat attggatgcc tacaaaccct
     4381 tgatggaaac tgacgaatcg ctgtgggatc tgatttatga gacgaatgtg aaaagcatgt
     4441 atcagctgac aaaaatcatc cttcctgata tgatccgcca aaaaagcgga acgatcatca
     4501 atatggcttc gattgctggc ttgattgctg gtggaggtgg gatcgcttat acaagcgcga
     4561 aacatgcgat tgtcggattt accaaacaac tcgctctgga ttacgcagga cagggtatcc
     4621 gagtaaaagg aattgctcca ggagccatcc aaacaccaat gaatgcggct gattttgaag
     4681 ggaaaggtga gatggcagag tgggtggcaa atgaaacacc agtcaagcga tgggcacagc
     4741 cggaagaagt agctgagctg acgttatttc ttgcctctcc tcaagcaagc tacatacaag
     4801 gaacgatcat cccaatcgac ggcggatggt tgttaaaata accttgtgtt tttttcaagc
     4861 ttctgtatga taaaactaaa gagagaaaca acggttgatg ataaaaaaag tcgatgactg
     4921 ttcccgacat tcgatgccga acagccagtt caaaaaattc tttttaataa tcgttaatat
     4981 tcgaaactaa gcggcttttc acgacctctt gatataaacg atattcaaaa gcttgcaact
     5041 gcggtaacaa gtccagccaa atgaaaaata taaggagcta tttttatttg accattctta
     5101 ttacttgttg cagggcagct ttttcactac cttctgcgac acttcagttg gaattttctt
     5161 cccatccttt ggtcgcggtc attaccaaga taaaattatc ttgcaaagga gaaatagaac
     5221 atggaaaaaa ataatacccg ttcagccaaa cgctggacaa cgacagatgt gactcggatg
     5281 gcgctagtca ctggtttata tgtggcagtg acgctggttt tgtctgtctt tagctttgga
     5341 gtgattcaga ttcgtctctc ggagatgttc aactatctgt ccttgtataa caaaagatac
     5401 atcgtcgcgg taacagcggg agtagctttg gctaatattg cttctcctct tggactgata
     5461 gatgttgcag taggaagcgt cagcacgctg cttgtattgc ttatcaacta taaaatcact
     5521 agtcgcatca aaaacatgaa aatcaaaatg gccgtgacag catgcgtatt tgctttttcc
     5581 atgtttacag tagctggtca gctaacgatt ttatatcaac tacctttttt cttgaattgg
     5641 tgggtgattg ctttaggaga attggcatca atgattgtcg gtgggataat tatttattgg
     5701 atcggtaaaa aagtggattt gtcaaaataa agatctgttt aatttaaaat agggcagcat
     5761 ggaagagact gaatgttgag tcttcttcca tgctgctttt ttgattaatt tgtatgaaga
     5821 aatcgattta gccgtttttt catcaagata ccgatataag ctgctccagt tgccttgatt
     5881 gcttccggca gtaaaaacgg cagtagtcca ctggcaaatg cagttgtaaa agtaagatca
     5941 ccacttattt tcagccagat cgttccgcag atcaatgcag caagagagcc cattagattg
     6001 gcaaagattg catataagta atgagcatcc gttctttcga tcatccaacc agttatccac
     6061 gtttgtaaga gaaatccaat caaatatccg ccagttggcc caaataaaac agctatacct
     6121 ccagtcatcc ctgaaaatac aggaagacca atcaagccaa gtaaaagata aactccaaca
     6181 gatatcaatc ctagttttct tccgagaaaa gttgctgtta atccaatagc aaatgtttgc
     6241 cctgtcaaag gaaccaaacc aaaaggaatg gtcatctgag aaaggacaga aataatcgcg
     6301 gcaaattccg cagcaagtat caattctttc aatgttcggt tcataacgcc ctcttttcgt
     6361 taacttaata tttttatttg gttaacgaaa ttgtaaaaca aagtgattac tttgtaaata
     6421 gaaaatataa aaaaagacca gctatccaat agatagcggt ctttttcaag aaagcttttg
     6481 aaattataag ctttggttgt agtattcaac gacaagagct tcgtcgattt ctggatatag
     6541 ttcgtcacgt tctggtaaac gagtcaatga accttccaat ttttcagcgt caaagcttac
     6601 gaatgctggg cgtccaacag ttgcttctac agcttctttt actgttacga tgttttgtga
     6661 tttttcacga actgagatca cttgacccac ttctacgtgg tatgaaggga tatcaacgcg
     6721 tttgccatca actaaaatgt gaccatggtt aactaattga cgtgcttgac gacgagtcgt
     6781 tgcaagtcct aaacggtaaa caacattgtc taaacgttgt tctagtaaga tcatgaagtt
     6841 aacaccatgt ttaccttctt tgattttgct agcttttacg aacaagttac ggaattgacg
     6901 ttcgttcatt ccgtacatgt gacgaagttt ttgtttttca gttaattgca tgccgtattc
     6961 tgattgtttg ccacggctat taggtccatg ttgacctggt gcgtaagggc gacgtgctaa
     7021 ttcttttcct gtgcctgaca atgagatgcc taagcgacga gaaattttcc atgatggtcc
     7081 tgtataacga gacattaaaa tttcctccaa tgaatataag tttttggagt aaaataatcc
     7141 gttgaaaaat tcataatcat atagtttgtt cttcaatctt cacctttgca gccgcggtta
     7201 cacaatcgaa cccgagacgg ggcaacaaac tagggatgag ttattatttt tctgctgcat
     7261 tattttacac aaattacagt atatgataga tcatccacga atgtcaagat aattagtaca
     7321 taaaaacgct ttacaaacct aggctgtctt tataaaatga gagggtatgt gcaaatggaa
     7381 aaaagcgaag gaagtttaag gagaaaatca atgaatccgg tatcaccatt atttgagaaa
     7441 atcgatgctt caattgaaaa gaaggcgaat ctttttgaaa acagcttttc acgttacgct
     7501 gttcgagcgg ttctcgcctg tttgtttttg actttaggaa cggctgtggc atttgcgatc
     7561 gcgatgaagg gggaagatat cgcgcatggt ctaggaaaga ttttatacgc atttatgttc
     7621 agctggggtc tggtcatgat tctatacatg aacgccgaat taggaacatc gaatatgctt
     7681 tatatgaccg tgggtgtttt tcggcagaga cttgctttaa gtaaggcagc caagattcta
     7741 tttgcctgta tttttttcaa tatgattggc ggtatattat gtggatttct aatttctatg
     7801 actacgccat ttcaagattt aacgttggac aactttatgt tagaatcaat agcagtaaaa
     7861 ctagcaaaac cttcagcaac gattttggtg gaagggatgt ttgcgaatat cgttgtcaat
     7921 accgctgtac tgatcagcat gcggatgaaa gaggatgtcg gaaaagtgat cacagtcgta
     7981 ttcatcattt tcatttttgc ttttttagga tatgaacacg tgatagcgaa ttttcctgcc
     8041 tttacgctta cttactttgc ttctcacgga cagatggatg gaatgacagt cagcaatgtg
     8101 ttacataatt tgttttttgc tttggcagga aattatattg gtggcggttt agtaatggga
     8161 ctgggatatg cttggctgaa tcagtcaaaa tcatcctatg tggactaaaa agtcgttcat
     8221 taaaggggaa gtgccatgga agttttagtt tttccaaaaa atgaggaaaa agcgttacaa
     8281 atggaaaagt acatgaaaaa tcttttttcc tttgctggag tacctaagcc ggaacgcgct
     8341 cttttacaac aaccgttact ggatgagagc aaaaatttat ctatttctga agttttagct
     8401 tttgttcatt attattatga acaaatcgaa agagagtatc agtatgtagc gatcgattta
     8461 gtccagaaaa acatcaaacg gcttacattt gaagacttag tcgtattgtt gccactagtt
     8521 cagaaaaaag aatggtggga cagcatcgac tcttggagaa aagtatacag cacatggtgt
     8581 aaagcctacc cagagcagcg tgaagaagtc tttcaattgt ttgaaaaaaa ggaagatttt
     8641 tggctacgtc gaatagcaat cacgttgcaa ttaggttata aacagcaaac agacacagga
     8701 ctattgatcc gagcaataga cgaagatcgt caaacaaagg aattctttat ccaaaaagca
     8761 attggttggg cattacgcga ttatagtaag accgatgccg attgggtaaa aagacagctg
     8821 acaacagatt tgagtaagtt agctgttcga gaggcaagta agtatttata agaaagcgag
     8881 acgacaagag atgagacgca aattatttgc ttttgatatt gatggaacgt tgttagattc
     8941 aaacaaacaa gctttagata gcacaagaga agcattggaa aaattacgca aagccggtca
     9001 tctggtcacg attgctacag ggcggagccg atttcatgcg caagagatca ttcgcgatct
     9061 tgattttacg aactacattt tatgtaatgg agcggccggt tttttagacc atgaacaagt
     9121 atataagaat ttactagatc gcgaccagtt ggatgcgttc gttcaagaag cgcatcatga
     9181 gcagatcgat acagcttttg tcagcttaga caatatcaag cgttcttctt ccaatgatgt
     9241 aacggtgatg gaagaagcga tgcattcttt cggtgctgtc ttgcctgaat tagatcagtt
     9301 ttttgcagag gaacaagaaa tataccaagc gttagcattt ttcgatcaaa catatgatgg
     9361 gaagttctct tcatatgata aaattcgatt tgtccgctgg catgagaaca gtgtagatgt
     9421 cgtacctaaa aatggttcta aagcagcgac aattctgcac ttagcagaac aagtaggcat
     9481 ccctcatgaa gacatcatca gctttggcga tggacaaaac gaccgtgaaa tgttgcgcat
     9541 gtcaggaatt gggatagcta tggggaatgc tgtgccagaa gtacaagcag aagctaagat
     9601 ggtcactgat acaaacgatc aggatggaat ttggaaggca ttgaaagagt taagcgtgat
     9661 ttaaaagagg ttgtgaaaaa gctgttctgc atcaagtaat aagcccagcc aaacaaaaat
     9721 agctcctcct atttttcgtt tttctgggct tattaccgca gatgcaagct ttcgaacacc
     9781 gtttaaggaa gaggtcgtga cagaagtggt cagtttcaag aaataagaag ggatttacga
     9841 aaattgcttc tcaaattttt gtaaaattac ggcttatttc tgaagaagta gatgaaacaa
     9901 cgtttattcg ggaaatggga ctgtgacaag atgtttgtca tagtcccatt tttttatttt
     9961 atttttcaaa actaagaaat accaggaaag agttcctttt gtttgacctt ttctttctta
    10021 ttgaaaacaa ctataatata gagtgatgtt aaaagagaag aggaatttag tgtgcgggta
    10081 ttgtttattg gtgatgtagt aggttcaatg ggacgagaga tgattactga atatcttcct
    10141 agattgaaga agaagtaccg tccgcaagta acgattgtaa atggtgagaa tgccgcttct
    10201 ggacgtggaa tcacagaaaa aatctataaa aaatttttgc aagacggcgt agatgtcgtg
    10261 acgatgggga atcatacgtg ggacaaccgc ggaatctttg agtttgtaaa tgaagctaaa
    10321 aagatggttc gtccggcaaa ttttccggaa gggacgcctg gacaagggat ggtctttgtc
    10381 aaagtgaacc aagtcgaact ggcagtgatc aacatgcagg ctcgttcttt tatggtggac
    10441 ttggatgatc cttttcgtaa aatgaatgag ctggtagaag aagccagaaa acgtacgccg
    10501 atcatttttg tagattttca tggagaaaca acaagtgaaa aacaagcaat gggctggttt
    10561 ttagatggga aagtttcagc agtggtcggt acacatacac acgttcagac aaatgatgcg
    10621 cgaattttgc caagaggaac agcgtatctg acagacgttg ggatgacagg tccctatgat
    10681 ggtattttgg gtatgcgcag agaacctgta atcgaaaagt ttctgactgc attaccaaaa
    10741 cgttttgaag ttgtggaaca aggccgatcg attttaagcg gctgtatctt ggaattggat
    10801 gatacgacag gtcatgcaaa agaaatccag ctgattcaga tcaacgatga tcgaccgttc
    10861 atggagtaga aagagaggca gttttttgca gcaggagccg gaaattcaaa aagaagtaag
    10921 gaaactgaca aaacttttaa gagagaatga aacgatcatt cgctacaaag aacttgaaga
    10981 aaagatccag caaaaccagt atttagctga gttgagagaa aaaatcaaac aagcgcaaaa
    11041 agatgctgtt cattttgccc actatgacaa accagcagca gaaaaagaag caatcaaaca
    11101 agcggatcaa ttcatgcaag aatttgatca gcatccgctt gttgtagctt atagaaaaca
    11161 gctgttggaa gcagatgatc tgcttcatca tttaacaact atgatacaag aagaaatcaa
    11221 tggacaaata gaggaggaaa aacatgcctc aaaaaactaa aaatactcct atgatggaac
    11281 aatatctaag catcaaggcc caataccaag atgctttttt gttctatcgt ttaggagact
    11341 tttatgaact attttacgaa gatgcgatca aagcgtcaca gcttttggaa ttaacgttga
    11401 cgagcagaaa tcgaaatgca gaagaaccta ttcctatgtg cggtgtacct caccatgcag
    11461 cacaaggata tatcgatgcc ttgattgaaa aaggctataa agtagcgatt tgcgagcagg
    11521 tagaagatcc aaagacgact aaagggatgg tcaagcgcga agtgatccag ctagtcactc
    11581 ctggtacagt catggacagc aaaggattgt cggcaaaaga caacaatttt ttaacagctg
    11641 tagttagtca aggaaatgaa tttggctttg cttatgccga tttaagtacg ggagaattga
    11701 agacggctcg gttgcatgat gaagaagctg ttttaaatga agcctctgca ttgcagacaa
    11761 aagaactcgt attaggcagt gagatcactg atacccttcg tgaacagcta agcgggcgtt
    11821 tagggctagt cttttctcaa caaaatgaga tggaagaaaa cgcggaattt agctttttga
    11881 caagcgaact agtcgatccg ctggaaaaag aagcaaccgg caaactattg acttatttat
    11941 cggtgacaca aaagcggagt ttagctcata tccaaaaagc agtagaatac caaccagatc
    12001 attttttgaa aatggattat tattcaaaat tcaacctaga attaagccaa tctattcgga
    12061 ctgggaaaaa gcacggcact cttctatggt tgttagatga aacaaaaaca gcaatgggtg
    12121 gccgtttact aaaacaatgg ctagaccgtc cgcttatcca ggcaaaacaa attaaagcaa
    12181 gacaagaaat ggtcgcttct ttgctagaca gctattttga acgtatcgat ttgcagtcag
    12241 cattaacgaa agtctatgat ctggaacgcc tagcaggtcg agtagctttc ggtaatgtca
    12301 atgggcgaga cttgatccag ctgaaaactt ctttagaaca agtgccaatg atcagagggt
    12361 tgattgaagg aatcgatcaa ggtcaatggc aaagtctgct ttcagaaatg cagccgatgg
    12421 accatctcgt ccaattgatc gaccaagcta tccaagatga tccgccgtta caaataacgg
    12481 aaggaaacat catcaaagat ggattcaatg agcagctcga tatgtaccgt tctgctatga
    12541 gaaacggaaa gaaatggttg gcagaacttg aagcaaaaga gcgtcaagaa acaggaatca
    12601 agaacttaaa aatcggattt aaccgcgttt tcggttatta tatagaaatt acaaaagcaa
    12661 atcttggaaa tgcagaactg gagaagtatg aacgaaaaca aactttagca aatgcagagc
    12721 gtttcatcac accagaacta aaagaattgg agacacaaat cctcgaagca gaagaaaaat
    12781 ctgttgattt agaatatcag ttgtttttag ctgtgagaga agaagtcaaa aaggctatcc
    12841 agccgctaca agtacttgca aaagcaatca gtgcagtaga tgtgctacaa agttttgcaa
    12901 cgatcagcga acgctatcag tatgttcggc cagaacttgt cagtgacaag catcagcttc
    12961 tgatcaaaga cggacgacat cctgtagtag aaaaagtatt aggacatcag gaatatatcc
    13021 ctaacagcgt agaaatggca gaagacgaaa tgatcttgct gattacagga ccgaatatgt
    13081 ctggtaaaag tacgtatatg cgacagttgg cattaactgt tctgatggcg caaatgggct
    13141 gtttcgttcc ggcaaaacaa gcagttttac ctatttttga tcggattttt acccgaattg
    13201 gtgcaagtga cgacttaatt gctggacaaa gtacttttat ggtagagatg atggaagcca
    13261 atcaagccct gcgttatgct acgccaaaca gtttgatttt gtttgacgaa ctagggcgag
    13321 gaacagcaac ctatgacgga atggcgttgg cccaagcaat catcgaatat atccatcgtc
    13381 atgtacaagc aaaaacactc ttttctactc attatcatga actgacggta cttgagaaag
    13441 aacttccgca gcttaaaaat gtccatgttg gagccgttga aaaagacgga gaagttgtct
    13501 tcttgcataa aatgatggat ggacctgcgg ataaaagcta cggcatacat gtggcaaaga
    13561 tcgcaggatt gccttccaat ctactagaac gagcagcaga tatcctgcat acgctagaaa
    13621 atggcgataa cggacaccat actgttccag ctgaagtaga agagaaaact aaaacagcca
    13681 aacaggagca aataaaacca cctgcagaaa aagtaaatca agaccatata caagaccgtg
    13741 taaaagaaga aaaccaacag ctttctttat tcagtgaatt atccgaaaat gaaacagaag
    13801 tcattgaatc actgaagcaa ataaatttac tagaaatgac gccaatggat gcactaaaca
    13861 cattatatga attacaaaaa caactgtagg aagaaggcgg atatcgatgg ccaaaatcca
    13921 agaattatct gaacgtttag ctaatcagat cgctgcggga gaagtagtgg agcgaccagc
    13981 ttccgttgtg aaagaattag tggaaaatgc aattgacgcc ggcagtactc agattgatat
    14041 cttagttgaa gaagcgggac tgcggacgat ccaagtgatt gataacggag aaggcatttt
    14101 ggcagacgac gtagagaatg ccttcaagcg gcatgcgaca agtaagatcc ataatcgcga
    14161 cgatcttttt cgcatacgaa cgctgggctt tcgaggggaa gctttgccaa gtattgcctc
    14221 tgtttctgaa atgactctgg aaacggcgaa tcaggaagaa aaacaaggca cctatctgtc
    14281 gctgaaaggc ggagaagtta ttgagcatcg accagcacca ctgcgtcaag gaacgaagat
    14341 taccgtcagc aatctgtttt tcaatacgcc agcccgcttg aaatacgtga aaacactgca
    14401 cacagaatta gcgaacatcg gggacatcgt caatcgactg gcattgagtc atccatcgat
    14461 tgcttttcgt ttagtacatg atggaaacaa aatgctggca actgctggaa atggcgatct
    14521 gaaacaaaca attgcgggaa tctacggatt atctaccgca aaaaaaatgc tgaaaatcga
    14581 aggaaaagat ctagattttg agttatttgg ctatgtttca ttgcctgagg tgactcgtgc
    14641 cagtaggaat tacttatcga cgatcatcaa tggacgtttt atcaagaact tttccttgaa
    14701 taaagcaatc gttgcaggtt atgggtcaaa attaatggtc ggtcgtttcc ctattgctgt
    14761 attggaaatc aaaatggacc cgcttttagt agatgtaaat gttcatccga cgaagcagga
    14821 agtccggctg tcgaaggaaa aagaactaac agagctaatt agtaacgcca ttcaaaaagc
    14881 attgtctcaa gaaaatctga ttccaagtgc agcggataat ctacggttta agaaaaaagt
    14941 acctgatcag cctaaaacag aacaaatcga aatcgatttg tcctacgctt ttgaagaaga
    15001 aaaacctaaa aagccaagta gtttgaattt tgatcgagag acaggtacct ttttcgtaga
    15061 agaactaaag aacacatttg ttgacagaat tgttgataag tctgtggaaa atgttgaaaa
    15121 ctattctggt ttttctgaag aaaacaccac ttatccagaa aaaacagcga aaacggtgga
    15181 taagtttggt gaaaatgttg ataactttgt agaaaaaaca gtggaaaaat ctgaaaagaa
    15241 agaatttccc attgtggata acatggagaa aacaaatgaa gaacaagcag ataaatccgt
    15301 agaagaggat gaaaagattt acgaggaaga attgacactc catcctgaat ttgatttcac
    15361 tcaaggaaac gacaaaacat tagaacaagc catcaagaaa ttagaaacgg aaaaaccagt
    15421 acaacgtttt cctcagttgg aatattttgg acagatgcac ggaacgtatc tttttgccca
    15481 aagtagcgat gggttgtaca tcattgatca gcatgctgcc caagagcgca ttaagtatga
    15541 atatttccgg gaaaaaattg gggaagtatc tgatgatctg caagaattgc ttgttccatt
    15601 tgtgttagac tatccaaaca gtgatgcttt gaaattaaaa gagcaaaaac acttgctaga
    15661 agatgcaggg atttatttag aagaatttgg tcaaaacagc tttattgtac gggcccatcc
    15721 tacttggtat cccaatggag aagaagaatc gattattcgg gagatgatcg atatgctgct
    15781 gacaactgga agtgtgagtg taaaaaaatt ccgtgaagca acagcaatca tgatgagttg
    15841 caaacgatcg attaaagcca accattattt gaatgaagca caagcacgtg tgctgctgaa
    15901 ggatctggca cgatgcgaaa atcctttcaa ttgtccccat ggccgtccag tattgattca
    15961 ctttaccaat tcggatatgg aaaggatgtt caaacgaatc caagacccgc attaagtcaa
    16021 agagaagagg gaaaacatga agattgtatt agcatcccaa tcaccaagaa gaaaagagtt
    16081 attggctcat atcgtttcag aattcgagat tgaacctgct gatatcgatg aaacgccatt
    16141 tccagacgaa cagcccgtcg attatgttcg tagaatggct gaagaaaaag cacgaactgt
    16201 ctgggaacaa gatgaaaaag aagaccattt agtgattgcc agtgacacga ctgttgttct
    16261 caatcaagag atcatgggta aaccagaaga tctggctgag gctgaattca tgctgaaaaa
    16321 actaagcggg gaaacacata ctgtatatac agctgttgtt ttaaaaacaa aggaacgaga
    16381 agaacggata ctagcagaag cacatgtcac tttttatcct ttgacagacg aagagatcaa
    16441 acgctacttg gagacggggg attatgcgga taaggcaggg gcatacggca tccaaaatgc
    16501 tgcgagtgta ttcgtcaaag aaatttctgg agattattac agtatcgtag gatttccgat
    16561 tggtgctgtc aatcaggcac tgaaaacatt catttaaaca aaaaagcgga ctatctgatt
    16621 ttcgattgaa aatccatgat agcccgtttt ttttattgaa aagattactt atctactctg
    16681 actaagattt tacttcagct ttaccgactg cttcgcccgg gcgaacaagt cctgattggt
    16741 caagagaaag tcctgcaact ttttcattat tggtcaaagc aacaataatg tcagttttct
    16801 tgccagcttg ttctatttct ggcaaatcca tttcagcaat cagggtctct ggtgtgacaa
    16861 ggtatccttc tttcacgaat acattgaaag gaacaccctt tagttcaaca gtatctaatc
    16921 ccatgtgaac aagtacttcc agcccctcat tagaaagaat accaatggca tgttttgttt
    16981 caaagacact cgtcacttta ccgtttactg gtgcatagac tttgccgttt gacggttcga
    17041 cagcataacc gtcacccatc attttttgtg agaaaactgg gtcatttact tcagttattt
    17101 ccttgatggt ccctgcagcg actgcgaata atgtttcttt tgttgcaact gccgtaccag
    17161 ttgctgaaac aggttcgaaa gactgtggcg ttgctgttgc gccatttgct gtttgaagtt
    17221 gtacatcagc agcattttca ggaacagcag gatcaatctt attcaaaatt ccctgacgtc
    17281 taaagatgat cgtcaagcca aatggaatga tgacagcaat gatcattgcg atgatgaaag
    17341 gtacccatgt ttctgcttgg atagctagta ttcctggcaa accgcctact ccgatcgcgt
    17401 tagctcgaac acccatcaag ttagcaaaca tgccagctaa tccagagccg accattgcag
    17461 cgacgaaagg atagacgtat ttcaaattga ttccgaacat agcaggttca gttacgccta
    17521 agtaacaaga aatcattgct ggaatcgaga tttgttcttc ttttttattc ccacgatgta
    17581 agaagatgat ggctaagact gcagagcctt gggcgatatt tgataaagca atcatcggcc
    17641 ataaattcgt tgatccgaaa tctgcaatca gctgcatatc gatggcattg gtcatatggt
    17701 ggagaccagt aatcactaaa ggagcataca ggaatccgaa tacggcacta aacaaccagc
    17761 tgatagaaga cgtcaatcca gtatttacga tatttgagat ccagcttccg actgtccagc
    17821 cgataggacc taaaataacg tgtgcagcta aaacagttgg taacaatgaa aacaatggaa
    17881 caaaaatcat cgagatcgac tgagggatat atttgcggaa gaagatttct agatacgcta
    17941 acataaaccc tgccagcatg gctggaataa cttgcgcttg ataaccaatc atctggactt
    18001 gagcaaaacc aaaatcccaa aatggaatat ctgctgcggc tgtggaagct acactataag
    18061 catttaataa ttgcggagaa actaacgtaa ttcctaaaac gattcctaat atttgtgtag
    18121 tacccatctt ttttgcaata ctccaagtga tacctactgg aagaaaatgg aaaattgctt
    18181 caccgggtaa ccacaaaaag gcattgacac cattccaaaa cttcgagtgt tcaacgattg
    18241 ttccaccaag actttcaaac tggatacctt caagtacatt tctgaatcca aggatcaatc
    18301 caccgacaat gattgcagga atcagtggtg taaagatttc tgctaaaacg gcaatagcac
    18361 gttgaacggg atgaaggttt tgttttgcag ctgcttttcc ttgttctttg gaaacccctt
    18421 caactccaga tactgcaacg aagtcgttat aaaaagtaga aacatcgttt cctatgatga
    18481 cttggaactg tcctgcattc gtgaacatcc cttttacgct aggtatatct tcgatcgctt
    18541 tttcgtccgc ttttcctgga tcattcagga caaaacgcat acgagtcgca caatgactaa
    18601 ctgcagcaat attttctttt ccgccgatat catgtaacag cttctcggca tctgcttgat
    18661 attttgccat atcaataaac ccctttctct taacttgtct atgcaagttg tttcttcatt
    18721 tactatactt tttttcggag agaattgcaa gcgttttatt aaaaagtacg agagaaaatg
    18781 atatcgtttt aaaaagctta aaataagctt ttcttaaaaa taggaacatt gaaatacaaa
    18841 aagagctgta gtatagtgaa gaaatgatgg aagaacgagt gagggaaaaa acatgaataa
    18901 gttcaatgaa atttttttag atttggaaca gaaaatttta gcgaaagaat atcctccgca
    18961 ttcgctttta cccagtgaga accaattgat caaaatttat ggtgtttcac gagaaacaat
    19021 tagaaaagcg ctgaatcttt tgactacgga aggttatatt caaaaaaaac aaggaaaagg
    19081 ttcgatcgta ctggatcgga atcgcttcga ctttcctatt tctggattga ctagctataa
    19141 agagctacag gagacccaac gtattccaag tgaaacgatc atccatacgt tagaagaaac
    19201 agaagtgaca aaagcattga acgagatcac tggctgggag atcggtgcgc ctgtttggca
    19261 tttgatacgc gaaagaaaaa tcaatggcga agtagttata ttagacaccg attatctatt
    19321 aaaggaaatc gttcctattt taactccagc acaagcacaa ggatcgatct atgaatattt
    19381 tgaaaatgag ctttctttga cgattgctta cgcgcaaaag gagatcacag tagaagaagt
    19441 aacggaaatc atgaaaacag cgatggatct tcacgatgac acttatgcag tagtagtcag
    19501 aagcctcgtt catctggaag atacccgctg tttcgagtat tctgagtcgg ttcaccgatt
    19561 agataaattc cgtttcgttg agtttgcccg acgccgaaaa gtgtaaatag ttcaaaagaa
    19621 agcaaattgt tggcatctgt ccactttggc gctatgatag gatcacaaag ggggcatttt
    19681 taatggataa accaataaga ccagaaaaag aagaactgaa agaaaagtta tcacccatgg
    19741 cttatgcagt cacacaagaa aatgcaacag aacgaccatt tagcgggaaa tatgatgatt
    19801 tttacgaaaa ggggatctat gtagatatcg tcagcggtga accgttattc agctctgcag
    19861 aaaaatatga tgcaggttgt ggctggccat cattcagcaa accaataacg aaaagacaag
    19921 tgcgcgaaaa agccgatttt tctcatggca tgcatcgaat agaagttcga agcaaagaag
    19981 cagattctca tctcggacat gtgtttaccg atggaccagt agatcaaggt ggtctacgtt
    20041 actgcatcaa ttctgcctca ctgaaattca ttccatatga aaagatggat gaattaggtt
    20101 atggggaatt caaatcactt gtagaataat ggaaatacac aaagagcggt acaatacggg
    20161 gatttactcg atcgtccgct ctttttttat tcaagcgatt gctatttgaa agaaacgctc
    20221 cctagactcc ttttttcaca aggtgaaatg tgctacaata aagggaacgt atgtgtgcaa
    20281 aagaaagagg actagaatat gtatgaatat ctgacaggtg tggttacctt tatcaacccc
    20341 tattacatag tagtagaaac caatggtatc ggctaccaga tcgcactagg aaatccttac
    20401 cgctacagta gcaaactaaa tcaaaaaata aaaatttatg tccatcaggt aatacgtgaa
    20461 gatgcgcatc ttctgtatgg atttgattcg ctagaagaaa agcaactgtt tttgcgatta
    20521 gtaagtgttt caggaatcgg tccgaaaagt gcccttgcaa tcatggcaag cgaagaccac
    20581 tccggtttga ttcaagcagt agaatccagt gatgtcactt atttgacgaa atttccagga
    20641 gtagggaaaa agacagcaca acaaatgatc ttggatctaa aaggaaaatt cggtgagctg
    20701 agtatcgata caccttttag tctatttgat gaagcaaaca caaaagacgc tacagctcta
    20761 tctgaagcaa tggaagctct ctcggctctt ggttacagtg atcgagaaat caaacgtgta
    20821 gaaaaacaat taaaagaagt agataatcag acaacggatg agtatctacg ccaagcactc
    20881 aagctaatga tgaaaaaata aggaggagac caaatgacag atgaatactt gttatcacca
    20941 gaatctggcg aaaatgaact gagtttggaa aaatctcttc gaccacagct gctttctcaa
    21001 tatatcggac agagaaaagt caaacaagaa cttcagattt acatcgaagc tgccagaaat
    21061 cgggaagaac ctttagatca tgttctgttg tacgggcctc ctggattagg aaaaacgacg
    21121 atggccatgg tcattgctaa tgagatgacc gtaaatattc gtacgacgag tggccctgct
    21181 atcgaacgtc cgggtgactt agtagcaatc ttgaatgaac tggaagcagg ggatgtcctg
    21241 tttatcgatg agatccatcg attacctaga gtggtagaag agatgcttta ttcagctatg
    21301 gaagatttct atgtagatat catggtagga caagggccaa ccgcacatcc ggttcatttc
    21361 cctcttcctc cctttacttt gattggcgcg acgacacggg caggtatgct ttctgcacct
    21421 ttacgagatc gatttggcat catttctcat atggaatatt atgacgaaca ggatctgcag
    21481 gagatcgttt tacgttcttc ggatattttt caaacagaga tcattgaaga aggggcattg
    21541 gaaattgctc gccgatcacg aggaacgcct agaatcgcta atcgcttgct caaacgggtt
    21601 cgtgattttg cgcaagtcca aggagacggg aaaatcgatc gtcctatagc ggatcaggca
    21661 cttactttgc tacaggtcga tcaggctgga ttggattatg ttgaccagaa attgttgcga
    21721 acaatgatcg agttatatgg cggcggacca gtagggttaa gtacgctttc agtaaacata
    21781 ggagaagagc gtgagacggt agaagatatg tatgaaccgt atctgatcca aaaaggattt
    21841 ttaaagcgta cacctagagg gcgaattgct actgcttatg cctatgaaca ttttggttac
    21901 gactatcata tatgattttt gcttttagat tggaggaaaa tgaatgagtg atacaacacg
    21961 tacgaaacaa ttgatcaaaa acgtattgat tgatttgtgc caaacgcaat cttttcgaaa
    22021 aatcagcgtc caaaaaatcg caaacgaagc aaaaatcaat cgacaaacat tttattatca
    22081 ttttacagat aaatacgatt tgcttcgttg ggtctattgt gaggatgcat tgcattattt
    22141 agatccacaa tgtctttcac ttgataattg ggaagagcaa gcattgaaaa tgctgaaagc
    22201 aattgctgag aagaaagaat tttatgcaaa tactgtgctt tctgacccag aaattttaca
    22261 aaaagagttt tctcaactga tccaaaaatc atttatcaag atttttgaac aaatggatga
    22321 agaaagacaa ctttctgcaa gagacaaact attttatgct cgtttttttt cttacggttg
    22381 cagtggtgta ttagtcaatt ggatcacgaa tgaatatgaa gaaacaccga tggaaatcgc
    22441 tatgcagctt ttccgtttag ccaaggatac ggaattatat tcttatcgtc tttatgcaaa
    22501 agaagagttg gaagaacagt aatagaaaaa ttttttgttg gaaaagcaat aatcgctaaa
    22561 aggagatggg gaaatgacaa aaattttatt tgtttgtttg ggaaatattt gccgttcacc
    22621 gatggcagaa ggcttgctga gaaaacgaat agcattagaa ggaagggaag cagaattttt
    22681 tgtggattct gctgctacaa gcacgtatga agttggaaag acaccgcacc ctggaacgaa
    22741 aaaaatatta aaccaagaaa aagtagatat gacgaatatg gttgctcggc aaatcacacc
    22801 ccacgatttt gaaacttttg attggatcat tggcatggat caagaaaatg tagaagaact
    22861 gaaacgaaga gcaccacgct cagcgcaggg gaaaatccat ttgtttttaa gtagcattcc
    22921 tggaaaagaa aaagaaaatg ttcccgaccc atactatacc aataactttg atgagacata
    22981 tcacttgatc acagaaggcc tgaatcattg gtggaatatt tggattgatg aaaataaaat
    23041 aacaaacaat ttgtaaagtt aacgctttct ttttaaggcg atgttagagc atttccttgt
    23101 ttttttgtaa aatgttaggt aaaataaaat taattaagta acactttctt agctaaagga
    23161 ggagtgcatt cgatgaacga agagacagta tcgccaatga agaagcttac gaagttagtt
    23221 actcatactt cgtatcaact ttttgattct tttgtaaatg acactgaaga acatgctaag
    23281 aatgtacgat tgcaaggaaa gtttattctt gagctgcaaa aagctgctac tgaacatagt
    23341 ctagttatct tacaagtaag agcaaatcgt ttgagtagca agtatgaaac gatctcaggc
    23401 tgggtggcga caaaaacggt cgaaaaagac cgagtgatga tccgcctaca aaacgaagag
    23461 cagcagatcc ggatggttcc tgtagaacaa attgaaaaaa taacaatcct ttcaccagca
    23521 ggggaacagg aacggttatc aaaataattt ataccagata gaaaagcacg tatgagcttg
    23581 taccatcaat cattactaat caaaaataac cgaatgcttt aagctgatat cagtaaaacg
    23641 gaacgtttta ggatgagtat cacagcatga cagcattggg ttgattagtt gaatcgattg
    23701 gatggtacaa gtttttttga taaaaaaagg tcctgctagc cggggagcta gcaggataag
    23761 gagttaaaaa tgaaaaagtg ttgttagggt tgtttgttgg tatgcttata taatagtcac
    23821 tagttgtgaa tattttgtga cgaaagtttt cgaaagttgt taagtttttg ggagcgtcaa
    23881 taattttgtg taaataaatt gtcctcctgc aaaataatta gttactcagt aaacattgaa
    23941 actaatgtat cggttacctg ttgaaaacct ttatggcttc tgtttagaaa tttttgattg
    24001 tatgtatcaa aaatgctgac tagaaagcgt tctagtgatt cttcattttg aaactgctct
    24061 tttctacggc tgtatctttt aatttgctta ttgaaagact cgattagatt ggttgagtaa
    24121 atggttctac gaatgctagg tggaaaatca taaaaagtta ataagtcttg gttttctatg
    24181 agtgactgcg tcactttagg atagtttttc ttccatttct caatcatgcc ggataagaag
    24241 gtattcgctt cttcttttga gttagcttga taaacagcct taaagtcatc acagatttct
    24301 tttcggtctt t
//
LOCUS       DAZKLA010000031        23666 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000031 DAZKLA010000000
VERSION     DAZKLA010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.031"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..639
                     /locus_tag="ACG85N_001062"
     CDS             7..639
                     /locus_tag="ACG85N_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223849.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(773..898)
                     /locus_tag="ACG85N_001063"
     CDS             complement(773..898)
                     /locus_tag="ACG85N_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFQ0223850.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1032..1925)
                     /locus_tag="ACG85N_001064"
     CDS             complement(1032..1925)
                     /locus_tag="ACG85N_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFQ0223851.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2107..3033)
                     /locus_tag="ACG85N_001065"
     CDS             complement(2107..3033)
                     /locus_tag="ACG85N_001065"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFQ0223852.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3118..3879)
                     /gene="pflA"
                     /locus_tag="ACG85N_001066"
     CDS             complement(3118..3879)
                     /gene="pflA"
                     /locus_tag="ACG85N_001066"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFQ0223853.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4123..6354)
                     /gene="pflB"
                     /locus_tag="ACG85N_001067"
     CDS             complement(4123..6354)
                     /gene="pflB"
                     /locus_tag="ACG85N_001067"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFQ0223854.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6631..9081)
                     /gene="parC"
                     /locus_tag="ACG85N_001068"
     CDS             complement(6631..9081)
                     /gene="parC"
                     /locus_tag="ACG85N_001068"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFQ0223855.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9093..11138)
                     /gene="parE"
                     /locus_tag="ACG85N_001069"
     CDS             complement(9093..11138)
                     /gene="parE"
                     /locus_tag="ACG85N_001069"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFQ0223856.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11304..11738)
                     /locus_tag="ACG85N_001070"
     CDS             complement(11304..11738)
                     /locus_tag="ACG85N_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFQ0223857.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11865..12503
                     /gene="plsY"
                     /locus_tag="ACG85N_001071"
     CDS             11865..12503
                     /gene="plsY"
                     /locus_tag="ACG85N_001071"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFQ0223858.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12635..13510)
                     /locus_tag="ACG85N_001072"
     CDS             complement(12635..13510)
                     /locus_tag="ACG85N_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFQ0223859.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13592..14383)
                     /gene="codY"
                     /locus_tag="ACG85N_001073"
     CDS             complement(13592..14383)
                     /gene="codY"
                     /locus_tag="ACG85N_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFQ0223860.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14401..15801)
                     /gene="hslU"
                     /locus_tag="ACG85N_001074"
     CDS             complement(14401..15801)
                     /gene="hslU"
                     /locus_tag="ACG85N_001074"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFQ0223861.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15815..16363)
                     /gene="hslV"
                     /locus_tag="ACG85N_001075"
     CDS             complement(15815..16363)
                     /gene="hslV"
                     /locus_tag="ACG85N_001075"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFQ0223862.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16400..17305)
                     /gene="xerC"
                     /locus_tag="ACG85N_001076"
     CDS             complement(16400..17305)
                     /gene="xerC"
                     /locus_tag="ACG85N_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFQ0223863.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17503..18879)
                     /gene="trmFO"
                     /locus_tag="ACG85N_001077"
     CDS             complement(17503..18879)
                     /gene="trmFO"
                     /locus_tag="ACG85N_001077"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFQ0223864.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18848..20926)
                     /gene="topA"
                     /locus_tag="ACG85N_001078"
     CDS             complement(18848..20926)
                     /gene="topA"
                     /locus_tag="ACG85N_001078"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFQ0223865.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21048..21905)
                     /gene="dprA"
                     /locus_tag="ACG85N_001079"
     CDS             complement(21048..21905)
                     /gene="dprA"
                     /locus_tag="ACG85N_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFQ0223866.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21961..22728)
                     /locus_tag="ACG85N_001080"
     CDS             complement(21961..22728)
                     /locus_tag="ACG85N_001080"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFQ0223867.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22721..23581)
                     /gene="ylqF"
                     /locus_tag="ACG85N_001081"
     CDS             complement(22721..23581)
                     /gene="ylqF"
                     /locus_tag="ACG85N_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFQ0223868.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         6153 a         5110 c         4070 g         8333 t
ORIGIN      
        1 gatattatgg aaattcaacg tagaaccact aattttttac atttaggctc tttttttgca
       61 actttgacga tcttattttt tctatgttat tcatacctta agacctcaca tgcgccttct
      121 agtgcattag gctggttgct tttggatcat tctgatatca cagaagccga agaaaatccg
      181 ttttttttga ttttgtcttc tctttgcggc ttactgtttt ctgtttattt cggtgcctat
      241 ttttttcatc gacatcctgg tacgttaaac gaagcttcaa gcgtattgat tgcatttaaa
      301 acgttttctt ttttagcaat tcttttttat tcgtttaatc acaatacgct tttcttcttt
      361 atcatgaatg gtttagtcgt gattttatcc atttttactt attttatttt ttcactcatt
      421 tttagccaat tgaaatgtcc gctcttcttt cgattagttc ctgtcacctg ttttatttac
      481 agcttgtttt ccttttttgt ccttgcttta atgccactga ccaacccttc gacgatccaa
      541 gtcgcaggaa atctgttgga tatgctcttc atcgtcagtc taggtgtatt tatgtggcaa
      601 agaaaaaaca aacaaaataa aaagcatttt gaagcctgac tattacgact tcataaatgc
      661 ttttttagtt ttaaacactt caatctaaaa gaataaccca agattggata aattattaat
      721 gactcgccat tcgtttctta gtgattctgg attatcaaat ggacttccta atctaatcag
      781 ctagttcttc ccataaaatg ccatacttga ctgaatcata atattgatta tttaaaaatc
      841 taacttttct tatcactact tcctttgtca ttccacattt ctcaccattt ttttgcattc
      901 ctttgtttcc agaccatgta gtgaatctaa taggtggtag ataggcaaaa tactcaaata
      961 attcttttaa ccataaagga agagaaaggc tcctcctgca agagtcagta taatttttgc
     1021 ttactgctct cttaggtttt actcactaaa actgttccat attctttgat ataagaaaat
     1081 gcatgcggta taaatttctt gctcagctca gaaaagattt gtcgtttaag aaaggctatt
     1141 tcctccattg tcaatgaagg atccaatgtt ggtaattcaa tctcaaaatt gtttttttcg
     1201 acaataggta attctaatgt gaatgattct tcttccttta tagtattcgt cacaagtaga
     1261 gactgatgga aaatactagg ccggcgattt ttatatttat tcttacgtat cctgtcaacg
     1321 ataacttcta tttgatcaaa aaagtacgat tcatttacgt ctccaataag ttcggctaat
     1381 tctttgaatg ccttcgttac aggaagctga ttcttctgta caaaaaagta attagccaaa
     1441 gtaagcggga agtgttctat agaggaaagt gtgataaaac ggtacttctc atttcctgca
     1501 gttgtcctca gcaattctgt ctctcggctg tttcttactc cataaaaata cgcttctttc
     1561 cgttcatctt cgaaacaata cgcccaaata acttctccca tcgctaattt ttgttcatca
     1621 acagagagtc tcttcaattg ttcagctatg gtttcagcag ccgaagtggc ttgttgcaag
     1681 taatctttag gatcaaatat tggaaagttc aaatgatatc ttcgttcttc cctcttgatt
     1741 aatcctgcat cgatcaataa atcaagaaaa tgggaaaatt tattttgcga aaattctttt
     1801 ttcaattctc ttagaatcgt ttcttgttct cgatgttcat atagataatc ggtcaatgaa
     1861 gaaaaaagcg gatcatcggt cagtttttga taaaagtttt ccttattttt tgaataatag
     1921 ttcatatatg ttctcttttc tatccgtttt attttactta aaaggctttt acgtaaaaag
     1981 gaggaagaac aagagtcata attgactgtt attctcctcc atttcttcgg cctttagtat
     2041 gttctatgtt tctttttata tcaatcgctt atccattcat tttgaatgct tcatactacc
     2101 atttgattag ctaaatacag ccgtcaattg aggtacgact tgttttttac gtgaaacgac
     2161 acctttaagc aaagcacggt tgttggtcaa agttgtttgg aaagcttctt ctactttttc
     2221 ttttccttta ccagcaacaa gtaattctga atcactgtct aagatgtttg tgacaactaa
     2281 tacgaacaga tcgtagccat ttgctttcat ttcatcttcc attgctgctt ctagctctgc
     2341 ttgtcgatcc atcacttcat taaggtccac tgtattgact tgagcgatac gaacgttgga
     2401 actgcccatt gggaaacttt ttgcatccaa atcaagaaga accgcagctg atttatcact
     2461 tagatttgtt cctgctttca acatttcaag tccataagtg tttagatcga cttcagcgat
     2521 tgctgcaagt tcttttgcag ctgcttcatc ttcaggcgta catgttggtg atttgaacaa
     2581 caatgtatct gaaatgatag ctgagagcat gattcctgcg atttctttag gaattgttac
     2641 ttggttttct ttatatagtt ttaaaataat cgtacttgta catccaactg gttctgcacg
     2701 atagtacaat ggattagctg tttcaaagtt tgcaatacga tggtggtcta ctacagctaa
     2761 aatatttact tcgctgatat ctgaaatact ttgttggaat tcattatgat ccacaagcat
     2821 gacatctgca ttttctgctt tagctgactc tactactcgt ggtgcagata gctgaaagtg
     2881 gtctaatgcg tattgtgttt cttcattagg tgtacctaga gcaactgctt ctgtttcttt
     2941 acctaattca ttttgcaagt gtgaaaacgc aatggctgcc ccgattgcat ctgtatctgg
     3001 attttggtga ccaaaaacta aaatttttga catgtcattc gctcctttgt gatttctcct
     3061 atttaataat agcaaaaaaa cggaaagaag caatacttat cttctttccg atgcgaccta
     3121 tcgcgttaaa tattttgtat actcttctgt atgaagcaat cgtttagcat tttcgacacg
     3181 ttcttttgta ggtggttcga tcccttctaa tggatatttc aaaccaagtg tttcccactt
     3241 gtattttccc atcgtatgat aaggaagaat ctctactctg tctacatttg acaaggtttg
     3301 aataaagttg tctagtcgga tcaaatattc atcataatca cttcgctcag gaactagtac
     3361 atggcgaatc caaaccggtt tgttgatttt tgacaaatat tgagccattt ctaaaatatt
     3421 ttcattgtcc caatgcgtta gttttttatg ctcttcatta tcgatgtgtt tgatgtcaaa
     3481 taacagtaaa tctgtatact tcatcaattc ttgaaaacgt gaaaagaaag gttcttcata
     3541 cgtaaaaggt tttccgcatg tatccaatgt agtatgcatc ttccgctctt ttgctttttt
     3601 aaatagttcg atcaaaaaat cgatctgcaa tagaggttct ccaccactaa cagtaatacc
     3661 acctttatct ccccagtaag gtctgaagcg ttctgcttta tctaaaagtt catctgctgt
     3721 gtattctttc cctccgccaa tattccaagt atctggattg tgacaaaatt ggcaacgcat
     3781 gcggcatcct tgcataaaca cgacaaagcg cagtccaggt ccgtctactg aaccgaaagt
     3841 ttcgatagaa tgaacatagc cgattgtttt ttcttccatt taagttcaca tcttttcttt
     3901 gatttcattt taagtacaag ttagctgtcg aagcgaagct ttctgaacaa aatttttcgc
     3961 aattgccgct tcgttatgtg taaaatttac agggacggaa catacaagtc ctgcccctgt
     4021 aaatcgtttc tttcaaataa gcatactgat tattcgattt tgatgcgatg gctattcaac
     4081 gtcctttcga cgctaagtcg aaaaactttt ttcacgaact ttttacattc tgtcgtgtga
     4141 agttctcatc aagacatcta attgttgttc tcttgtcaag tcacggaatt taactgcata
     4201 accagatacc cggatcgtca agttcggata tttcattgga ttttccattg catcacgcaa
     4261 taaatcatta ctgaatacat tgacattcaa atgataagct ccttttgaga agtatccatc
     4321 taagacatta cgcaagtttt cgattcttgt atcttgttct tttccaagac ctgaaggatt
     4381 gattgtttgg gtatttgaaa ttccatccaa tgcacagccg taattcattt tagctgttga
     4441 gttcaggcta gctaataacc cactttgttc cgcattgtaa cttggatttg ctcctggcgc
     4501 aagtggtttt ccagcttcac ggccatcagg agtatttcct gtatttttac cgtaaacaac
     4561 atttgaagta atcgtcaata atgaagttgt tggttttgag ttgcggtaag ttttatgacg
     4621 tttgatttgt cccatgaagt attccaagat ccatgaaact aattcatctg cacggtcatc
     4681 atcatttccg tatttaggga agtcgccatc cacttcaaaa tcaactgcta ttcctttttc
     4741 attacggatt gcttttactt ttgcatattt gatcgctgac aagctgtctg ccgcatgaga
     4801 gatcccagca atacctgtag caaatgtgcg ttgcaagtca gaatccatca aagccagctg
     4861 agctgcttca taagaatatt tatcatgcat ataatgaatg atgttcaacg tattgacata
     4921 tagttctgct aaccaatcca tcatattcat gaagttatgt ttcacttctt caaagtctag
     4981 tatttctgat gtgattggac gatattctgg tccaacttgg gcgccagtca cttcatcgac
     5041 tccgccattg atggcatata atagtgtttt agccaagtta gcacgagcgc caaagaattg
     5101 catgtctttt ccgacaactg ttcctgatac acagcaagca atcgcacaat catcagaacc
     5161 ccattgttca cgaagctgat tatcattttc gaattgaatc gaagaacttt cgatagcgat
     5221 tctgctagca tatgttctga atccttctgg aaggtgttca gaatacaaaa cagtcaagtt
     5281 tggttctggt gaaggtccca tatttgttaa tgtatgaagc agacggaaat cattctttgt
     5341 aactaatgaa cgaccatcta atcccatacc tgcaatggat aaagtagccc agatagggaa
     5401 tcctgagaat aattggttat attcaggtgt acgcgcgaat ttgaccatac gtaatttcat
     5461 tactaagtgg tcgatcaatt cttgtgcttc tttttctgtg attactccat tatccaaatc
     5521 acgttggata tagatatcaa ggaatgctga gatacgtccg attgacatcg cagcaccatt
     5581 ttgtgatttg attgctgcta aatatccgaa gtatagccat tggacagctt cttttgcatt
     5641 tgcagctggt ttagaaatat catagccata tttcgcagcc atttctttca tttgcgctaa
     5701 tgcacgatat tgttcagtga tttcttcacg taaacgaatc acttcttcac tcattgtatg
     5761 ataaccagtc atatcatgat cttttttctt ttgttccatc aaatagtcga ttccatacaa
     5821 tgccacacga cgatagtcac cgatgatacg tccgcgaccg taagcgtctg gaagccctgt
     5881 aatgattttg tttttacgag cagcacgcat ttcagcagta taagcgtcaa atacaccttg
     5941 attatgtgtt ttgcgccagt cagtgaaaat atgtgtcaat tcatcgtttg gtgtatagcc
     6001 attgcttttt agcgcattgt tagccatatt gattccgccg aacggcatga atgcttgttt
     6061 caaaggtttg tctgtctgta gaccgacgat tttttccaaa tctttatcca aatagcctgc
     6121 atcgtgtgaa gtaatagttg atacgatttc tgtgtccata tcatagacac cgttacgctc
     6181 gaattggata tcatttaact tttgcaattc atcccataat ttttctgttg cttctgttgg
     6241 gccttccaaa aagctgtcat caccgcgata ttcagtgtag ttattttgaa taaagtctct
     6301 agtgtctacg ccttctttcc atttggtacc tttaaatcca ttccattgct ccattgtatg
     6361 gacctcctaa atttctgttt tatgtaacag tttttacgtg attagtataa cacagcaccc
     6421 tatctttgca agggttttct tgttttttat ttaacaattt ttatgtttta tttacatata
     6481 tttaaatcaa cgttgacata ccaacattta agcattgtga acactttcat ctttttttgc
     6541 aaacgtataa ggatttatta aaaatgaatg aaaaagagac gccacctgta ttaatacaga
     6601 tggcgtctct tcattaaaat actacaacag ttacgactcg atgaaaatct caggagcatc
     6661 gataacttcg atgacttctc cacctttttt ctcatccatc gcaaatgatc cattcgatgt
     6721 tcgatcacct atagggaaat cagttgggtc aatcgttaac tgctgccctt tttgattcat
     6781 caaggttatt tcttttgatc ttccttccga catatagata atacggtgcg gatttttttt
     6841 cagctcacgc atgaccgtca ctccccgctt cgcacgtgaa gtttggctga tttcttgagc
     6901 aagcatgcgt ttaacagctc ctcgctgagt aacgattacg atcggcgtat caccttctgc
     6961 taacacaagc agcccgttga taatcctatc tccatctttc aaattcatag acttcactcc
     7021 agctgccttt gctccaacaa cgggaacatc tgttaaagga tagcgcaaac ccattccttg
     7081 atgcgttact aagaaaacat ctaagcctgg ctgatcttct ttcagataca cagcaatcag
     7141 ttcatcttct tccgccttta gcttcataca gctgagcgga cggctcttat aggtgcgcca
     7201 tggttcgaac tctgtcattc gtgtttgctt gatcaatcca ttcttgctga tgaatacaaa
     7261 tgttttctct gcttgaattt tttgatatgg gaaaacagct aaaatcgatt cgtctacaga
     7321 cagattggag attgtttgag aaatgtgctc accagcatct ttccatttca aatcgggtag
     7381 ttcatggaca gggcggtaga tcacatttgc tttatttgtg atcagaagaa catggtccaa
     7441 tgtactcaat tcacctgagt atagcaagta gtcgccttct cgcatcccga tttcttctgg
     7501 tttggaagca ctataagaac gaaggcttgt tcgcttcaca tagccttctc gcgtcacact
     7561 gacaattacg tcttcttgtg ccactaagac ttgcgtatca attttgattt cttcgatttc
     7621 gtcttcgatt gtcgttagac gagcggaaga ataatttttt ttcacttctc gcaattcttt
     7681 tttcataaca gagaaaagct ctttgtcatt tgaaaggatt ttattcaact ctgtgatttg
     7741 cgcaatcaag ctttcggatt ccttttgaag ctctgtgata tccgtgttcg tcaaacggta
     7801 taattgcaat gtgacaattg cttctgcttg ttcttctgta aattgaaaga cgtcgactaa
     7861 attctttttg gcgtcttttt tgtctttact ttcacgaatc gttgcgatta cttcatccag
     7921 aatcgataaa gctttcatca atccttctac gatatgctgt cttttttgag ctttggctaa
     7981 atcgaattga ctacgttttg ttatgacttg ctttcgatgc tcgatataac ttcttaaaat
     8041 gtctttcagt ccaacttgat gcggagtcat atggtcgata gcgaccatat tgaaattgta
     8101 attgatctgt agctcagtgt ttttgaacaa ataattcaaa attccttgag cattggcgtc
     8161 ttttttcaat tcgacgacaa tttgcagacc tgttctatca ctttcatctc gtacttcagc
     8221 tattccatcg attttcttat tcaaacggat ctcatccatt tttttcacca aggtcgcttt
     8281 atttacttca tatgggattt ctgtgatcac gatttgctgt ttattaccct tcattgcctc
     8341 tatctttgtt ttcgaacgaa gaataacttt tcctcgtcct gtttcataag cttttttgat
     8401 ttcatctttt ccttgcaaga tccctcctgt tggaaagtca ggtccaggaa tatattccat
     8461 caatttttca aggctcgctt gtggatgatc gatcatatag attgtgccat cgatcacttc
     8521 agctaagtta tgagtcggga tttctgttgc atatccagca gatatacctg tcgatccatt
     8581 gacgagaagg tttgggtatc gagctggaag gactgttggt tctttttctg tatcatcaaa
     8641 attccacacg agatcgactg tttctttctc gatatctgct agcatctctc cacttaactt
     8701 ggataatctg gcttcagtat aacgcatcgc agcaggtgga tcaccgtcca tactcccgtt
     8761 attcccgtgc atttcgatca gtacttcccg caacttccag tcttggctca tgcggaccat
     8821 cgcttcataa atactgatgt caccatgagg atgataattc cccatgatat ttccgacaga
     8881 ttttgctgat ttacggaatc ctttgtcata cgtattgcca tctttgttca tggcaaataa
     8941 tatccggcgt tgcactggct tcaatccatc acggatatca ggcagtgcac gttcttggat
     9001 aatatatttg gaataacgtc cgaagcggtc acccatcact tcttctaaag ttaattcttg
     9061 tacttcttgt cgattttcca tcaggtgacc tcctattcta cttcaaataa ctgattgttt
     9121 tcatttttat ctgctcgttc ttcatctaat aaatcattag aaactgaagg agagatttca
     9181 gtatcctctt ttttatctaa gatactgcca tcttcttcga gcgtaaattg cacgtgattt
     9241 tcgatccatt ttcttcgagg ttctactttg tcacccatca gtgtggttac tcgacgttca
     9301 gcttgcgcag catcatcgat acggacgcgg atcaacgtac gggaagtcgg atccatcgtt
     9361 gtttcccaca attgttccgc attcatctct ccaagtcctt tatatcgctg cagcatatat
     9421 cctttgccga cttttttaat cattgctgcc aattcgtcat ctgtccacgc atattcaatg
     9481 acttgcttct ttccttgtcc tttggatact ttatataaag gcggtaacgc gatatatact
     9541 ttcccagctt caatcagcgg tttcatataa cgataaaaga aagtaagcag taatacttga
     9601 atatgggcgc cgtctgtatc cgcatcggtc atgatgatta ctttgtcgta attacagtct
     9661 tcgatagaaa actcaggacc gactcctgca ccaatcgtat agatcatcgt attgatttct
     9721 tcatttttca agatatcttg cattttagct ttttcggtat tgatgacttt tcctcgtaaa
     9781 ggtagaattg cttggaactt tctgtctctt ccttgtttag cagatccgcc agcagaatcc
     9841 ccttcgacta aatacaattc atttttctta gggtttcggg attgtgcggg tgtcaatttt
     9901 cctgaaagaa gagattcgcc ttttttacgc ttcttcccat ttcggctttc ttctctggct
     9961 ttacgtgctg cttctctcgc ttctctggct ttgattgctt tacggaccaa catctggctc
    10021 atttcgctat tttcctgcaa ataaaagccc atttgttcac tgatgacgtt atctactacg
    10081 gtacgagcaa ctggcgtacc aagtttttct tttgtttgcc cttcaaattg cagaagattt
    10141 tcaggtacac ggatagaaag tacagcagct aacccttctc tgaaatcact accttccaga
    10201 ttcttgtccc gttctttcag caaaccaact tttctcgcat attcattata agatttcgtc
    10261 atcgccgctt tcataccagc ttcatgggtt cccccatctt ttgtccgaac gttattgaca
    10321 aaagataaaa cattttctga ataaccgtca ttgtattgat aagcgacctc tacttcgata
    10381 ccttcttttt ctcctgaaaa ataaacaact ggtgtcaatg tatctttttc ttcattcaga
    10441 taatcaacaa attctttgat tccttcttcg taatgaaaaa tttccttgac tggttcttct
    10501 ccacgaagat cagaaagaga gattttaacg ccttttagaa gaaaggcaga ttctcttaac
    10561 cgttcggcta aaatttcata tgaaaatttc gttgtactga atattgtatc atctggtaaa
    10621 aaatggacag tcgtcccgtt tgcttttttt gtttttccta tctttttcaa agtaccgtca
    10681 ggtttaccgc cgtttttaaa tttctgctga tactctacgc catctctaac gatcgttaca
    10741 attagccatt tcgataaggc atttaccaca ctggctccta caccgtgcag tccaccagaa
    10801 gttttgtacc ctccttgacc aaactttccg ccagcatgca gcacagtaaa aatcacttca
    10861 actgttggta ttccggaagc atgcattcct acaggcatcc cacgtccgct atctgcaaca
    10921 gttatactat tgtcttcatg gatggtcaca tcaatctcac tgccataacc tgataatgct
    10981 tcgtcaactg cattatcgac gatttcatag actaaatgat gcaagcctcg gctgtcagtg
    11041 gatccaatgt acatccctgg tctttttcta actgcctcta atccttccaa tacttggatg
    11101 gacgagtcat tgtattcgtt gtttactttt tttgccaagg caaacgctcc ttcaataata
    11161 ctttgcggca aaaccgcacc acgattatca tactagaaaa ggtcgtgaaa aaaaagaggg
    11221 tagacgagaa atcaatttta attatctcct aaccgctttt tcgacattcc cctctatcga
    11281 tcatctcttc tttgtggtca tttttatttt tggttttctt tcaacttacc aagttcaatc
    11341 ttaatacaac gatccattac gatatctgta cgtcctgctt ttcttagcat ctcagctgct
    11401 tctttgtttt caatccctaa ttgtgcccag aacactttag catcagtttt caaaaaatca
    11461 cctgctattt caggtaaaaa ttcactccgt ctaaatacat ctacgatatc gattggatca
    11521 tcgatttctg tcaaattcgc atagactttc tcgcctagaa tttcttcacc agccaataat
    11581 ggatttactg gtatgatttt atatccattg tcttgcaata cctgtgcaac catgtaactt
    11641 gttcgttctg gtttattgct caagcctacg acggcaatat tttttgcttc ttccaaatag
    11701 ttgaaaatcg tttcttgtgt tggatttttt acagacattt ctgccacccc ttgcttttct
    11761 ttttattcca tctattatac ttgtatcttt cattttttca attgttcctg ttacctattc
    11821 atgctaaaat aaaggatagt tttcaacttg aaagagggta aaaaatgaaa ttgatcttat
    11881 tacttatttt agcatatctt ttaggttcta ttccttccgg tgtctggatt ggcaaattgt
    11941 ttttcaaaaa agatatccga caacacggga gcggtaatac tggaacgacc aatacatttc
    12001 gtgtattagg caaaacagcc gggattgttg tcctgcttat ggatatttta aaaggtacat
    12061 tggctacttg tttgccgatg atcttccacc tttctgggat taaccctctt tggtttgggg
    12121 tatgcgccat tcttggccat acatttccga tttttgctgg gttcaaagga ggaaaagctg
    12181 ttgcgacaag tgcaggaatg cttttaggat ttaatcctgt attttttgtt tattcttgta
    12241 tcatctttat cgttagttta ttttgtacaa gtatggtgag tttgagcagt atgatcagtg
    12301 cagtattgat cactttatct acgatcgtcc ttccttatgt agctcctgtg attttggcta
    12361 agcccaattg gttactgacg atcattgcct ttttagtttc ttcctttatc ttttatcggc
    12421 ataaagacaa tatcaagcgt atcagaaacc atactgaaag tcgaatcccg tttggtttga
    12481 atagcagcaa gaaaaaatcc tgatactttt tcattccaaa gagataaaac gggagtgtga
    12541 caaatcgatg tcacactccc ttattactta caggatagct tttttacaca ccttggtaaa
    12601 cggtgttccc tgctaacttc tcggtcacaa cctcttactt cactgtgatg gaatattttg
    12661 tcttgaagat ttcatgggca cctagttgat tgatccctac tttttccatc aagtttccac
    12721 tactgttcac cgtgtcagcg attccgcacc acggttcgat acaaacgaaa ggcgcttctt
    12781 ttggataagg agaccagatc cctacataag gcatatcttt atagcttaaa gttacgctat
    12841 gggaagattt ttcacttcga atggcgtacg catttagacc ccttgtttca aaaaccaagg
    12901 catcttgctc aaaaagttca cgattcaaag ccagattcgt atttgtctga cctaacgtct
    12961 tttgatccat atcgatgaat gggcctttca atggtaggcg taatcttgat ttcaatggag
    13021 aaaatgccag atagaaatct tcaaatgaca atccttcttc aagaggtaca ttgaaagcag
    13081 gatgtccacc gatagaaaag aacatttctt tttcaccggt atttgccact tgataatgaa
    13141 ccgtgatgcc gtctcctcct aattcatagc tgatcactaa ttcaaaatca aatggatact
    13201 tttctaatgt ctccggcgta ctccttaaga tgaaactagc catttcttgt ccatgttcaa
    13261 tgacttcaaa atccatatcc cgagcaaatc cgtgttgccc catttgatat gttttccctt
    13321 gataaacata ctgatcatct tttaaacgtc caacaaaagg gaataaaacc ggtgcatgtc
    13381 tgccccaata cgctggatca ccttgccaga tatactctaa accatgttct tttgatttca
    13441 gactgatcaa ttccgcaccg tgttcagcga tttcggcgat cagctggtca ttttgaataa
    13501 taacagtcat tgccattcct ccaacttcga cttacttttt aaaatgggaa ggctggaaca
    13561 aacatttcta tctgccccag tccccttact tctaataact gtgttttgct aactcgtctt
    13621 taaaacggga attcaatact tttaaataag ttcccttcat gcctaacgaa cgagattcaa
    13681 tgattccagc tgattccaat tttcgtaaag cattgacgat cacagaacga gtgatgccga
    13741 tactatcggc aatattagaa gcagtcagac gaccttcatc tccatccaat gcttcaaaga
    13801 tagcttgtac agccttcaat tcgctataag ataatgtgtt gattgccatt tggacagccg
    13861 ttgcactccg tacatctgct tcgatgcttc gagacttctg ataaaggatc tgcataccga
    13921 caaccgtagc tccatattct gctaagacta gatcgtcatc tgcaaacgat tgttccatac
    13981 gagataagat aatcgtaccc aaacgttcgc cagctccaaa gatcggaacg acagtcgtca
    14041 gaccaaatgg atatttctct cttgactcta ccggaaatgc agtaatatca ctagtaattg
    14101 ggatattagc ttcagttttc atcaactgtg ccactgtatc agtataagat tcaggaaatt
    14161 gtttctcaac aaacattgtt ttgattcgtt cattgttcac atcgtgacgt tcattataac
    14221 ctagtaatac tccgtcactt cctatgatat aggtgttgct gtcaagaata ccacctagtg
    14281 tgacagccat tttatcgtaa ggcaaatctg cttgcatgtc aaaagtattc ttttgctgca
    14341 aaagtgtgtt tatcttgcgg gttttcgtta gcaagtctgt cattgtcgtc ctccgatttt
    14401 ttataaaata tagcgactta aatcttcatt tgccgcaatt gagccaagtt tgtcatccac
    14461 atatgcttct gtgatcgtta tttctcccat ttgcatatct ggtgcttcaa ataatagatc
    14521 ttctagtagt ttttccaaaa tggtatgcag tctgcgtgcg ccgatattat ctgtgtcacg
    14581 gttgacatcg aaagcgattt gagcgatccg ttcaattgct tcttttgtaa acgtaactga
    14641 cacattttct gtaccgatca acgcgacgta ttgttttact aaagcattat ttggttcggt
    14701 cagaatcttc acaaaatctt cagctgtcaa atcatctaat tctacccgta tcgggaagcg
    14761 tccttgaagt tctggaatta gatcacttgg tttagacaaa tggaaagctc ctgaagcaat
    14821 gaataggacg tgatctgttt ggatgatccc gtattttgtg tttacttgtg aaccttcgac
    14881 aattggcaat atgtctcttt ggacgccttc tctggatact tctccagatt gctgtgattt
    14941 agaagtgatc ttatcgattt catcgataaa aataattcca ctttcttccg ctaatttgat
    15001 tgcctcacta tggatatcag catccttgac taattttgct gattcttcct tgatcaataa
    15061 ctctaaagct tctttgactg ttaccgtccg ttccactttt ttcttcggtg tcaaagcacc
    15121 tagagtttca tttaggtcga tccccatttg ctccaatcca ttgttcatca ttggtgcagg
    15181 tttcttttgg tcttcaactt caatggttac ttcacgacta tccagtaaac ccttttgtag
    15241 ttgttccaag actgtacggc gattgattcg aatatcttct gtcacttctt cttgcggttc
    15301 agcggtattt tgctgactag cattaaaaag attcatcatt tgttcaaatt gattagtgga
    15361 ttgtttctgt tcttttttga taccaggtac caagattttt accaaacgtt tttctgcatt
    15421 ttttttagca cttgtgtata cacggctgta ctgctctttt tctacgattt ggatcgcatt
    15481 ttctactaaa tcccgaacca ttgattctac atctcttccg acataaccga cttcagtaaa
    15541 ttttgttgct tcgactttga caaatggtgc attgacgatt tttgccaaac gtcgtgcgat
    15601 ttctgtttta ccgacccctg taggtccgat catcagcata ttttttggtg taatatcttg
    15661 ttgcattttt tcatctaact gcaaacggcg atagcggtta cgcaaagcaa cggcaacaga
    15721 tttttttgct gcgtgctgtc ctacgatata ttcatctaat tcacttacga tttgtcttgg
    15781 tgtttgtttt aattcataca tatctactca cctcttacag ttcttctacg ataatattat
    15841 gattagtaaa gatacagata tctgcggcaa tatttaacgc attttctgca atttctttcg
    15901 cactcatgtc gttttgagca tagtttttca ttgctcgggc tgcagccaac gcaaaattcc
    15961 ctcctgatcc gatagctaag ataccatcat ctggtgtgat aacttctcct gtacctgaaa
    16021 caagaagcat ttctttatca ttcattacga tcagcatcgc ttcaagtttt tgcatagatt
    16081 gctgtgtccg ccattcttga gctaattcga cagctgcgcg ttgtagattc ccgttatatt
    16141 cgtttagttt gccttcaaat ttttcttcta aagtaaacgc atctgcaaca cttccggcga
    16201 aacctacaac aacttctcca ttgtagatac gtcgtacttt ctttgcagtg cctttcatga
    16261 caacagattc acccattgtt acttggccat ctccagccat agcaaatttc ccgtcttttt
    16321 cgacggcaca gatagtggtt gaatgaaatg gtgattcgac catgtttgtt tcctcctaaa
    16381 tttgttcttt tccttatttt tacgctcttg gatggaaaga acgatagttt ttttgtaaac
    16441 tttcttttgt tacatgggca tagatctggg tagtcgataa attggcatgt cctagcaatt
    16501 cttggactgt ccgcaaatct gccccattat ttaataaatg agtagcaaat gtatggcgca
    16561 gcatgtgagg atggatatca ctatccaaac tgcttttttt gatcatttga ttcaagacat
    16621 attcaattcc agtaggcgtg attggttttc catgatgatt cacaaaaacg atatcatgct
    16681 cttcgtgata ttttgtcatc aatacctggc ggccttccct gaaatattct tgcagagcat
    16741 cttgagcaaa agaacctaga ggagcatatc tctctttgtt ccctttacca tggatcaaca
    16801 tgacctctga atcaaaatca atgtctgata ccgacaaact gctacactca cttaaacgga
    16861 tgcctgaccc gtaaaggact tccagtaaag cgcggttcct caaatccaaa ggactatctc
    16921 ctgctacgct gtcaaaaagt gcttgcattt cattttcata aaaaaaacgt ggcagtctca
    16981 aatttttctt tttgagatga acgtaagaaa atggattttc ttttatcact tcttctttga
    17041 gaagatactg ataaaacgaa cgtaggctgg ctatcttgcg actgatagaa tttctgctat
    17101 agctaggacg atgatcagct aatgcactca aatagacacg aaccgtaaaa tattccgcct
    17161 tcagcagatc agcgtctcca gattcctcca aaaaatgttt gaaatggaga atgtcttctt
    17221 catatgcttc tttcgttttt tctgaatatc ctctttcgac aatcaaataa cgcaaaaatt
    17281 tttgttccca cgtggcttct gtcattttct caccttcctt ttgtcccaga gaataattta
    17341 gcacagcgtt tataaaaaga caaataaatt gtcagaattt aaccaatatt tctaagtttg
    17401 ttcacaattg gcgcaattcc tcacattttg tttgagaaat tcttaagaaa aacgcaagaa
    17461 aaagagcgag actatccgtc tcaccctctt catggagttt ccttactcat ttgggattgc
    17521 agttttttct aattctgttt ttattgcctc attagctttt aaagctcgtt gtgcaattgc
    17581 ttcatatctt gcttttttgt ctcgaatgcg ctctggcagt tcaggaaaca gaccatagtt
    17641 ggcattcatc ggttggaaat gttttccttc cgcatgagta atgtagtaag ccatacttcc
    17701 aatagctgtt tcttgtggaa actcgactgg ttccatttct tttgctaagc gtgcggcgtt
    17761 gattccagca agtagtccgc tcgctgcact ttcaacatag ccttctacac cagtcatctg
    17821 acctgcaaag aacagatctt cgcgtttcgt tgattggtaa gtcggtttca gcaattctgg
    17881 cgaattcata aagctgtttc ggtgcatcac accatagcga acgaattcag cattttctaa
    17941 cccaggaatc atccggaaaa cacgtttttg ctcgccccat ttcaagtgag tctgaaagcc
    18001 cacgatgttg tataaagaag ctgcagcatt gtcttgacgc agctggataa cagcataagg
    18061 acgtttgcca gttttaggat cttccaatcc aactggtttc ataggtccga aaagcatcgt
    18121 tttgaatcct cgctgtgcca ttacttcaat tggcatacat ccttcaaaat atttttcttt
    18181 ctcaaaagat ttaagttctg ccacttcggc attgattagt gcctcatgga aagctttgaa
    18241 ttcctcttct gtcatcggac agttcaagta agctgcttct cctttgtcat agcgggactt
    18301 caaatatact ttatccatat cgatcgtagc tttgtctacg attggtgcag ctgcatcata
    18361 gaaataaaaa ccatctgaac cattgaatgc tttgatgctt tctgctaatg gatgagaagt
    18421 caaaggtcct gttgctacta cggtgattcc ttccggtatt tcagtaattt cttcgtttat
    18481 cactgttact aatggatgct ctttgacttt tcgggtaatt gtttcagaaa aagaatcgcg
    18541 gtcaacagct aaggcaccac cagctggcac tgctgtttca tctgccgaag tgattactac
    18601 cgaattcaat cggcgcattt cttctttcaa taaccctact gcattagcca aactgtttcc
    18661 tcttaatgaa ttggaacata ctagttcagc aaaattactt gtgtgatgcg cctcagtcga
    18721 tttgactgga cgcatttcat ataaacggac agggactcct gcttgtgcga tctgccaagc
    18781 agcttcactt ccggcaagcc ctgctccaac aacatttacc attttcaatg aaagaacctc
    18841 ttttctatta tttttggatg tcttcttcgt aatctccatt tacacagact acttgtttac
    18901 cgcctttgac tttcttctcg accagatatc cgtcacattt tggacaattt ctgcccactg
    18961 gtttatccca agaagtgaat tcacattctg ggaaacggga acaaccatag aatagtctgt
    19021 ttttcttaga ttttcgttca atgacttgtc cttcatgaca tactgggcaa gtcacaccga
    19081 tttcttttac gattggtttc gtattccgac aatcaggaaa attactgcaa gcataaaact
    19141 tcccatattt ccctaatttt atcaccatcg gatgtccaca gacatcacaa tcgaatcctg
    19201 ctggttcatc tttgatttga attttttcga ttttttcttc cgcattcgtc agttcttttt
    19261 caaacggttt atagaaacga tcaaccactt tgacccacgc ttccgtgcct tcttcaattt
    19321 tatccaaatc tccttccatt tctgctgtga aatggatatc gacgatttga gggaagaatt
    19381 cacagattaa tgaattgaca atttctccta gttcagtcgg ctcaaatctt ttttgtgtca
    19441 attttacata gtagcgtctt tggatcgtat caagtgttgg tgcataagtg gaaggtcgcc
    19501 ctaccccgtt ttcttctagt gcacgaatca acgtagcctc actaaaacgt gctggtggtt
    19561 gagtaaaatg ttgttttggt tcaatatcaa cagattggac tacatcgcct tcttcaagtt
    19621 ctggaagaat attttctttc tcttctttgc cgtcatctct accttccaca tacacttgca
    19681 tgaatccttt gaatttgatt tttgaaccgt tcgcaatgaa gatgacacca ttttgttcta
    19741 atgtcacttt cattgtatct aaaatagctg gtgtcatctg actagccaca aaacgtgacc
    19801 agatcaatgt ataaagtttc aattgatctt tatctaaata ttgtttgagt tcatctggtg
    19861 tacgaagcac actcgttgga cgcacagctt catgggcatc ttgtgcgcct tgagcatttt
    19921 tgatctttcg gtgatcatgt ctagaaaatt cttttccata cgtatcttca ataaatgctg
    19981 cggcctctgc ttttgctgta tcggcaatcc gggtagaatc agtacgcatg tatgtaatca
    20041 agccgactgt tccttgttta cccaaagtga ttccttcata taattgctgt gctaccatca
    20101 tggtttttct tgttcgaaag tttaacttac gggcagcttc ttgttgcagg ctacttgtag
    20161 tgaaaggatt cgcagggttt cttttgcgct ctttcttttc tactttttta acttcgaagt
    20221 ctgttccatc aattctagca gtgacttctt ttacactttg tgcatctggt aatttctttt
    20281 tcttaccgtc gattccccag aaatttgctt tgaatttctt gcgagctttt ttaaaattac
    20341 cgtcgatgct ccaatattct tctggaacaa attctcggat ctctttttca cggtcaataa
    20401 taatttttaa agcgacagat tggacacgtc ctgcacttaa ccctttcttg accttacgcc
    20461 agagaatagg actgatcgaa taaccaacga ttctgtctaa ggtacgacga gcttgctgtg
    20521 catctactaa atctacatcg atcgttcttg gttccttaaa agctgctttg actgcgtctt
    20581 ttgtgatttc attgaagacc acccgatttt tttctttcag atccaaccca agtaggtacg
    20641 ctaaatgcca agcaatcgct tctccttctc tatccgggtc acttgccaag taaacttttt
    20701 gagctttttt tgctgcggct ttcaggcttt tgatgacatc gccttttccg cgtatagaaa
    20761 tataatgggg ttcataattg ttttcaaaat cgatacccat tttacttttc ggtaaatctc
    20821 ttatatggcc aacactggcc attactttat agtttcgtcc aagatatttc tcaattgtct
    20881 tggcttttgc aggtgattct acaatcacta aatatttata cgccattact gaatggactc
    20941 ctttccattg tcgtgttcct actattaaaa gaggattcat acaaatcgta gctaatcata
    21001 tccattcaga aaacgtttgt caagaaaagc aaatgaaaag tatggtgtta ttctttaaat
    21061 tctgccaaga tatcttcacc cgaaaccgtg caaactgcac cgtcttggat caaatgatga
    21121 catccggtag aatgttctgt taaaatattt cctggaacag aaaaaactgt tcttccaaga
    21181 tccaatgctt gctgtgctgt tatcagagag ccgctttttt tcttggcttc aaagacacat
    21241 gttcctttag acaaagctgc gataatccga tttctcattg gaaaacaatg ctttttcggg
    21301 ccggattgag gaggatattc actcaaaaga agatgatcat ttgcaatcac ctcttgcaag
    21361 tcacgatttt cagcaggata gacttgctct atccctgttc caatgactgc gattgtccgt
    21421 ccaccatgag agattgctgc ttggtgaccg tatgtatcta tccctttcgc gcagccactg
    21481 acgatcacat atccagcttc tatcactggt tttagtaaat aataaactgt ttttttggct
    21541 aaagcagaag tgtgtcttcc accaacaacg cttaacatgt tacaagaaag taaagataga
    21601 tcaccattat aaaacagtac aattggcgga taggtcaagt gaagcagctg agggggatat
    21661 ttgggatcaa agcaagtgat aaacgactgt ttcgattgga ttttgcccca gttagcgtga
    21721 atttcctgcc aacttttgcg aaacagctcc cggtgtttgg ttattttacc tatcttaatg
    21781 atgtcttcaa atccgatttc tttataagga tactgcatca tccagttagc aagtgcccat
    21841 tttccttgac tactgattcc ttttgtataa gcaagtttca ataaattttc ttctatttga
    21901 tacatcataa aaccgccttt cgtctctctt tctaataaga tacgtaaaag cggctgtttt
    21961 ttattgtaaa tattttttta caggtgcaaa acttttacga tggattggcg taattccttg
    22021 tcgttcaagt ccttctaagt gttcctttgt tccataacct gcattatgcg agaaaccata
    22081 tccaggataa agcacgtcat attctttcat caattggtct cgatatactt tagcaatgat
    22141 acttgcagca gcaatcgaca cagatcgtgc atcccctttg attatttttt cctgctgaat
    22201 aggcaaatcc aaggtcatcg catcgatcaa caaatagctt ggtacaatgg acaatttttc
    22261 tactgcttgg atcatcgcta atttacttgc ttcataaata ttcacatcat cgattgtctg
    22321 ctgatcaacg atcccgatcc cgattgccag ggctttttct tgaacttgtt gataaagaag
    22381 ttctcgcttt ttctctgata attgtttgga gtcattcagc cctaatatga tctcattttt
    22441 cggcaagata actgcagctg ccacgacagg tccagctaat ggtcctcttc ctacttcatc
    22501 aatgccggca atccattggt gaccttgtgc gtacccttgc ttttcaaagc aaaacatttc
    22561 ttcatatttt tccttaatta atgcttcctt tttcaatcgt cgttcaaaag aagccagtgc
    22621 ttgctgcacg cctttgcgtg gatcatttct taatgattct agtgtaggat catctattct
    22681 ctgtatgctt ttcaatcgtt ctttgatact ttggatggat tcagtcatca cttttttgct
    22741 ccatttcttc gcaacgatcc agtgtgtatc gtccaagttt tccttgacga atatcatgca
    22801 cgactaattc gctgccacga tcatagtctt ctttataccc acgttttgct gtaatatcca
    22861 tcagtaattc cggtgccaat ttttcttctt gctcgattga taaaccataa cgatcgctga
    22921 ttcttcctga ataatatcgg gcaaaaaagt ctaatccgta aatcgctaaa tcatctaaat
    22981 ggacaagctg gtctttgatc gcgcctgtca gcgctagttt tttgccgatt tcttcatctt
    23041 caaatttggg ccataaaatc cctggtgtgt ctaacaattc caactcatta cctaaacgaa
    23101 gccactgctg accttttgtt acgccaggct tatttcctgt ctgtgcaatt ttcttgccta
    23161 ctagtcgatt catcaacgta gatttcccaa catttggaat acctaagacc attgcccgaa
    23221 tagcgcgagg cttgatcccc cgagcagcat ctcttgcccg tttttctttc aatgcttctt
    23281 tagccgcagg tactagacct ttaattcctt tactttcttg tgccgtgata gctaatgcaa
    23341 gatggccttg tttacgaaaa tattcttgcc acagctttgt ctgttgtgga tctgccagat
    23401 ccgctttatt caataaaaca aggcgaggtt tttgctgaat gatctgatct agcattggat
    23461 tacgagaaga taaaggtaat cgtgcgtcta ccaattcaaa tacgatgtcc acatatttca
    23521 ttttttcaga aacttctcgt cgggcttttg ccatatgtcc cggaaaccat tggatggtca
    23581 ttgttctctc cattcctttc ataccggaat attccccggc tttacttgtt tttttctttt
    23641 tcaatataaa ttcacttttt tattat
//
LOCUS       DAZKLA010000032        23204 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000032 DAZKLA010000000
VERSION     DAZKLA010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.032"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..329)
                     /locus_tag="ACG85N_001082"
     CDS             complement(<1..329)
                     /locus_tag="ACG85N_001082"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223869.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(343..600)
                     /locus_tag="ACG85N_001083"
     CDS             complement(343..600)
                     /locus_tag="ACG85N_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223870.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(795..1337)
                     /locus_tag="ACG85N_001084"
     CDS             complement(795..1337)
                     /locus_tag="ACG85N_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFQ0223871.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(1358..2449)
                     /locus_tag="ACG85N_001085"
     CDS             complement(1358..2449)
                     /locus_tag="ACG85N_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0223872.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(2457..4667)
                     /locus_tag="ACG85N_001086"
     CDS             complement(2457..4667)
                     /locus_tag="ACG85N_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0223873.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(4683..7211)
                     /locus_tag="ACG85N_001087"
     CDS             complement(4683..7211)
                     /locus_tag="ACG85N_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0223874.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(7263..7673)
                     /locus_tag="ACG85N_001088"
     CDS             complement(7263..7673)
                     /locus_tag="ACG85N_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFQ0223875.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(7686..7904)
                     /locus_tag="ACG85N_001089"
     CDS             complement(7686..7904)
                     /locus_tag="ACG85N_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFQ0223876.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(7901..8893)
                     /locus_tag="ACG85N_001090"
     CDS             complement(7901..8893)
                     /locus_tag="ACG85N_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0223877.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(8972..9883)
                     /locus_tag="ACG85N_001091"
     CDS             complement(8972..9883)
                     /locus_tag="ACG85N_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0223878.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(9902..10246)
                     /locus_tag="ACG85N_001092"
     CDS             complement(9902..10246)
                     /locus_tag="ACG85N_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223879.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(10266..10544)
                     /locus_tag="ACG85N_001093"
     CDS             complement(10266..10544)
                     /locus_tag="ACG85N_001093"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223880.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVMQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(10903..11403)
                     /locus_tag="ACG85N_001094"
     CDS             complement(10903..11403)
                     /locus_tag="ACG85N_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223881.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(11404..11949)
                     /locus_tag="ACG85N_001095"
     CDS             complement(11404..11949)
                     /locus_tag="ACG85N_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223882.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(11946..13202)
                     /locus_tag="ACG85N_001096"
     CDS             complement(11946..13202)
                     /locus_tag="ACG85N_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0223883.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(13563..13874)
                     /locus_tag="ACG85N_001097"
     CDS             complement(13563..13874)
                     /locus_tag="ACG85N_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223884.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(13897..15372)
                     /locus_tag="ACG85N_001098"
     CDS             complement(13897..15372)
                     /locus_tag="ACG85N_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFQ0223885.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(15821..16273)
                     /locus_tag="ACG85N_001099"
     CDS             complement(15821..16273)
                     /locus_tag="ACG85N_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223886.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(16275..16640)
                     /locus_tag="ACG85N_001100"
     CDS             complement(16275..16640)
                     /locus_tag="ACG85N_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223887.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(16739..21022)
                     /locus_tag="ACG85N_001101"
     CDS             complement(16739..21022)
                     /locus_tag="ACG85N_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFQ0223888.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(21169..21408)
                     /locus_tag="ACG85N_001102"
     CDS             complement(21169..21408)
                     /locus_tag="ACG85N_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223889.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(21498..21740)
                     /locus_tag="ACG85N_001103"
                     /pseudo
     CDS             complement(21498..21740)
                     /locus_tag="ACG85N_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21853..22104)
                     /locus_tag="ACG85N_001104"
     CDS             complement(21853..22104)
                     /locus_tag="ACG85N_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223890.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            22267..>23204
                     /locus_tag="ACG85N_001105"
     CDS             22267..>23204
                     /locus_tag="ACG85N_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0223891.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTK"
BASE COUNT         6853 a         4991 c         3503 g         7857 t
ORIGIN      
        1 gaattcattc cccaaaggcc ttttaagatt gatacagcag tccgttcact tcttttagac
       61 ttgtcataca agtcttttaa atgatcccat atcagactag tttgtctatc tagcttcaat
      121 ccattaaacg ctaatgaacg gttcattttt tctattactt tagataaagc tacagaaatt
      181 tttatacctt cacaacgcgc atcttggatt tcaataatag tatcatcaat tagtttttga
      241 atttcttgcc tatcaacata ttcaatctta gaaagagaat cttttaaagc tactaatgat
      301 tcaacgattt cttttttctc ttcagacatt ttcttcctcc ttctagtgac ccgtaaagcc
      361 atgtagagca gagtccattg aaccagtaat tacactatct acaacgccat tccaatttac
      421 tctacatttt ccatttttca gatttgtcgg tccatacgga tagccttttt gacattcaat
      481 gttcggatta taccagatag gattatacca ttcagccgca gaagcagatt cagtcgatac
      541 aaacaatccc acaccaacaa atccaataca catcacacta tagccaatta gttttttcat
      601 ctcgtatcca tctcctaaaa aatagaatag ttcttatagt tattcataag acatattttt
      661 tccgtttttg ttttatcctt tctgtcctat aacaactttt ttcaagtaaa gaatgacata
      721 gtttttctta aaaaaacagt ctgttttttt ctatttcttc ggtgtataat aaatatgagt
      781 gttcattttt tattttaata cgcactccgt acataataaa tcttgatcgc atcaatcttt
      841 tgagcttctt gatctacttt caattgaagt aatacaaatt gagtaacctt attcattgtt
      901 tcttctccaa ataacaaata ttgatccgga ttctctatat cttgtgtcac ctggtaaagt
      961 ttcccagttg ttttcatttg tacatgcggg tctacatcaa tactattgtc tttaatacaa
     1021 cgatctgtca tatagtccct cacagcttga ttgcgtttat aaacatctgt gaaattactc
     1081 cattttctag caaaatcttt taagaaattt tttgtcattt catctatttg catttgttgc
     1141 gctgtctcgt cttctaatga accaatttga ctgttacttt cagtactttg aagagttcca
     1201 cttgtttctt ttactgcttc gattgtttct tctgtcgttc tcaattgtgt tttttgtttt
     1261 tcagaagaaa accaatgaca accagtaaga cctatcaaac ataaccaact aaatactaaa
     1321 attatccctt ttttcacgtt tctagtcact gcctttctta tttcacctta cgaaccagac
     1381 tacgaatttt tgtttgattg tacgtgcgtt gataccgagc cataatccgt ccttgctcgg
     1441 cattctgttc cacactagag aaatgttgtc cgccatatga aacatctgtt ataattccgg
     1501 tgtgaccata gattccagga gacaatgcac cacctgcttg ccaattcacc acatctcctg
     1561 cttgtaaatc acttggtttt gggttcatga ttacttgcca accataggtt gtccagttat
     1621 aatcttcccc tattaacatc gcaaacatgt gtccagaacc cattaactgt gggccaccta
     1681 attgagatac ataccatgcc gtaccaccat aacattcccc attatttacg gatcgtccaa
     1741 tgatccgatc caaaaccttc gcattgcctt tactttttcc tgcaataagc gaagtttttt
     1801 ttgttggtag gctattccaa tgcttgatcg caaaagtatc atattccgtt aagttatagg
     1861 acgttatgat actcattgct cctcgataat acggactccc ttcggtaata cctgctgccg
     1921 catagcctcc acgacctaga atcaataaac aacttccagg atctttattg cccacacatt
     1981 gattaaagtt gctttggctc gccaccataa agccataata gtcataacaa gcttttaagt
     2041 tcggaaactt catatagaaa ccaccttcag aaccaccaat tccacgtgca gaaccttgtg
     2101 ggtagggaca accaggaaac caggtaatcc caaaaaagtt cgtatcgttt cttccagatt
     2161 gtgactgtcc ataagctgat tcatagccta attgagaaag aatcacactc ggtaataact
     2221 gatactttac agctgcacca actgctgctt gcgcatgttc atctgtgatt ggaccacgtc
     2281 ctttaatctc tgaaaaaatc ggtaatcctt cggtatattc cccattaaaa gcgacagcta
     2341 gacctgtagc ttctcctcca tgctcagaaa gttctcctga aaaaaagaat ggcaatagaa
     2401 aaaagggaag aataaagaaa agaagacaac ctagacaccc tttcttcatt tcttcctcat
     2461 cctttcttcg ataatggttt ttggactgtt cgtccttttg gtaaagtaaa atcagttcct
     2521 ggttttgcgt ccaaaatcgg cagtgtttta ggatcctttt ctattttttc cgattgctct
     2581 gttggcttat ctaaagaaga aaatgcaggt tgtaacgatt ttggtgcgga aatcgacggt
     2641 ttactttcag acgaacgttg ccccggttgt tgcacagaac gatttcctga tagagtggct
     2701 accgacgaat taggaacaga gtattggttc aataactgct gcattgctgg ataactccca
     2761 tttttcgcct gatttttcac ttggcgaatc tgttgtgaaa gagaaccagc agctgttaat
     2821 tgtggcacgg gtacttttcc agaaggcaac ggaatcgatt gaccaacaaa agcgatacct
     2881 ttatttaaca atggttttct ttgattattc ccttgttgct tactcaattg tgccaatgat
     2941 tgtacctgtt gttttggtct tactgaattc atggacgaat ttggaatcaa accatttgaa
     3001 agaccagaat tccactctgt tttcgatttg gtagaagaca tttcaggctc taccccatga
     3061 taacgaacat gacgaccact ataaggcaag gcttgatttg aacgcccacc tagttcaaag
     3121 ggattaacca cttgcctccc ttttaccgat tcttgtggtg tacgtttttg ttgaaacttt
     3181 tgaaatccat gtatcgctgc agaagcgagt ggattcgttg ctcgatacac attccctgct
     3241 aatcctgcac ctttgattgc caaattttgt ccagcagttg atagttgacc aggcagttgt
     3301 ttcatttgag acaaagaatg acttgctatg tttttcgtgt tgtttatagt ttgatctgca
     3361 gctcctaacc ctagtttctt taataaatct ttccgtttta caatgagtaa aattgatagg
     3421 agtgctttga caaacatatt tcctaaaaaa ccaaaaacag tattcattgg aattaaatcg
     3481 tctactatgt tatagatcaa aaatgccacc aaaataatca cactgtaaaa agatttttga
     3541 aacatcaagc ccaaaaagga ctttaaggta ttcatgagca tataatcaaa tactggaaca
     3601 aaagaagcga gtaatgaaaa aggaatcaca agtaataaac acaacaccaa catttgaaag
     3661 ataaaacgaa tcatgccaat cactacaagt ggtaatccaa tacttagatt aagcaatggc
     3721 accatagaac caacgactgc tttataaaca gctttagttg gttgaatata gacacgataa
     3781 gccttctttt tccctctatc ttctcctgca gcagtggtca cttttgtctc aatatcagat
     3841 aattcttcac ttgacgcttc cttggctaga aattcttgtg gttcaatctc tgcttgatac
     3901 aattcttcta aatttgttgt cccataattt aacaatatat acggatcaac aacagccgct
     3961 ttataataaa gttggcgcac tttttgaatg gcttcctttt gtgtcgttgg cactaaaaca
     4021 ccattgacat cttttgtcgc caattcatct agactatttg tagcagaaaa aataaagcct
     4081 tctacctcca cggacaaatc atttaaccct ttggtaaaag cttctccttt actaggtacg
     4141 ttactaccat agccaaacca aaccattgaa aaaatgacaa cgaagagaaa tcgaataaac
     4201 accatttttg cgtaatgttt ccctctaaat atacgaagag taaatagata taacacatac
     4261 aagacgacaa tccccgcacc aataggatcg tatatttttg aataaatagt atccgaaaaa
     4321 gtgaaaaact ggtgaattaa ctcgttcatt gcagctcctt catagagctt ctcaatgaca
     4381 aaatcaaggc cttgccaaat caatttactt aaactaaaga acatattcgt catcattttt
     4441 agaaaatctg tgccatttgc cccaaataac attttgccta atgaaaattc actatcagat
     4501 aaataagaag aaaaggtatc aatatgaaat ttcccaatct catagatcga ttgatccacc
     4561 tgcttttctg ccgcatacac caatccacca acaaaaagca gtccgaatcc aatcgttaaa
     4621 aaaaacaaaa cgcaccattt cttttcctta tgctttatat aagccattgt ttcacctcat
     4681 ttctattgca tgattaacgt atcctgttct tctacttgag agtgacttgt tttttccgtt
     4741 gtttgtaatg cagcggtcat ttcttcaaac aaactgtgta cattgagttt tcctacacgt
     4801 ccatagggat ctaacatcca acactgtcct ttaatcatgt ggctcatttc agctaagttc
     4861 gcttcattta cttccaaacg taaatgattc aaaatctttt ctcgctcact gtcttcatca
     4921 aaagcaaaga cgacaccaaa attccctttt tctttatctg aacctaagtc accaataaat
     4981 tgtgtatcta acactaattg attcatctgg gaacgcccca cacgtgccat tgctgcaata
     5041 atactacgtc ctgcgttcga attattaaat acccatgctt ctgtaaaata aatcgctgtt
     5101 ttcttactac tatctcgttt tccaaacatt tcacaaaaac gacctaaact aatcatcaaa
     5161 cacaaagact ttcgatccgc atctgtatat agttctggtc ggacattgtc atttggtaat
     5221 ttcaaatctt ggatctctaa aatcgttgtt ttggcattaa agtctaatcc cgggttttct
     5281 ccataagaaa atcctaaacg taaaatcgaa tcttctaccg tcaagcgtaa aaactcacca
     5341 tattctcgaa cctctttttg ttccatttgt tctaaatagt cgaccacatg catcaagcca
     5401 acttgttgtc cggcttcttt ttgaactaaa acttcttgga ttccctttaa taccgcccct
     5461 tttaaaaggt gattggtacc taacggactt aactcatcaa tcatatcctg ggctacttgt
     5521 ttcgcatcta ctcgggataa acaaacaatt ggatctaata caccataatt acggggattt
     5581 gtagcatcta acgtgacata actcattttt tgcaaatgtt ctacaaacaa agggtaatgt
     5641 ttctgataat acgggtcttg aatgagtttc gtaaaccatt tgcgcttttc tcctttggga
     5701 tccacaaata aactttgtac attcataaac gaactataca aaaagagcaa gccaactaaa
     5761 aaggtcttcc ctttaccggt ttttccagtt actgctacat gaggcgcatc aaacaacgca
     5821 cctttgattc cttccgcaat ggctagaaga ttcaaaaaga caaaacgatt tgacgcataa
     5881 attaattctt ctaatggtgt tttttctcct cgagaaccac catcccctaa tgtatcttgc
     5941 cgaccaatat accagccgga ttccatacct aattctgata ctgctgaaaa caaccattcg
     6001 ccaactgctt ctacagttgt tcggtgcttc caatgtaact gccgtcccaa tggttgccct
     6061 aataaacatt tatagaacaa ttctcgttga tcattttttg cacggtggat atggataatc
     6121 gtatgaagtt gttgaataac cgttgcacta ttttgacgaa cttcttcaac cgtttgaccg
     6181 tatacaataa aacatgcgat ccaatcgacg acaggttgcc cattatctac tcgttgcttt
     6241 aaatgtttta ctaatcctcg aacagttaat aagcgttcac taggttcttc atcattttga
     6301 accaattcct tatgctctgt tttgaaccgt tccgatagcc aattcagttt taaaccaata
     6361 cctccctgtc ctttcaagcg tggatagtga attttcattc gcaattctgc tgggaaagaa
     6421 agatcctgag caaattgaaa tagatcacaa tttctcatat tttcaggtgt ttccccaata
     6481 ggcaacaatg ctaaataact actccctaac tcatcttcta attgaagaag gcctttatgt
     6541 tccttcggta tacgtacata cgttccttga ttgtctttca gtacttccca attattttgt
     6601 tcttctactt tgtgagccat tcctcgaata tattgcagac gattgagata caacagctct
     6661 ttttttacta agggacgaat agaaaagcta taaagtaatt gtgtcacttc ttttacataa
     6721 ggatcacttt cagaaaacaa agtttccaaa tcatctactt ctaatccaaa taagcgtgct
     6781 aaacttcctg ctaaatctaa ccagattaga tttattacat catgaaaact ctccaccgtt
     6841 gctttttctt tgactttgat tcctaaaata aatgaaggac gatagacttg accttctcgt
     6901 tccaacaatg tcactgtttc ttttgcataa tacgcaccaa ttgcttgact atcgggagaa
     6961 aatccctttc ctaatgtgtc aaacttcgtc cgtaaattca ttgttcgtgg atacatcacc
     7021 aactccacat cttcataatc tgctaaaagc aacagtaaac gagccatcat ttcttttatt
     7081 tcctgtgtct tttttggtgt gttttttgaa attgttcgtg gaaaaacatg atagtaggcc
     7141 cagatacaat cctctccatt taacaaacaa tttttcttga atgcttttac tggttctttt
     7201 acaagcacca atgaatttca cctcccaata aaaaaggaac aaccagttga ttgtcccttt
     7261 ttctaatcct ctaaataaac atacttattc acaaaaagta attcttccaa tgcattgttt
     7321 gataccttgt cccagttata atacacacaa tcaccattca tcatacgaat catataacaa
     7381 aaccaatccc ataaataaaa atgcactttt tttccatcag gttttagttt tgccagtgct
     7441 aacacacata aataggggag tcctatataa aaaacaactc ctgctggtgg aaaaaaagaa
     7501 atgacccaac caataaatga acgtaaaaat aaatattcaa agattacgac caacaaaaaa
     7561 gttgctgcat tgactaatgc aactggaaat ggaagacgcc aattgcttgt aatctgttgt
     7621 acgcgataag gttccgcata aacaatcgaa taatcataag gttcccaatc cacttcacta
     7681 tcctcctaat ccataaataa tttttcaatg attttaccaa acgcatcaaa tacacgtgtt
     7741 ggatcattta agaagaaata aactgcggca cctgctgcga tagatattaa tgcatttcct
     7801 acttttgatt tcataaaatt tcgcaatccc agataaacaa ccaccattat taaaaaatag
     7861 ccaccttgcg ttaatataaa atctacaatt cccttcagtg tcatttccgt tcttctcctt
     7921 tcctatccaa ccaattatgt gttaattttt caacataata ctgatttgtt tttttcgtaa
     7981 tgacaatcgt catttgttct ttttgtaaaa gtgacgtatc accatctcga aagacgaccg
     8041 ttgcttttac aataaaaggt tttttcgttt ctttataacg caatactttc atctctactt
     8101 gatcaacttt agctgtttga ttgagtcctt caggttcttt catcatgtat gacatatctt
     8161 ttgcattgtt tttcgcatac ttatctaaga aatcttccac aaatccttca acatctttcg
     8221 cttccgacgc atcaaccgtt tcgtataatg tgtcatcata agtaatcatt cgttgttttt
     8281 ctgtattctt tggtagtgcc gtaaaataag gataagcaat aatacgacag cccttgtttg
     8341 aaatagaaaa gggaagattg agtacttgtg tgattgtcac acttgtttct ttcttactct
     8401 ctggattcat cgagcgaata tcataagtta ctttataact agcagttgga atggtttcaa
     8461 tttcttttaa tccaataaac gaagcagcag ttaattttcg aacacctgaa acacttcctg
     8521 gttcttgaat aaagttttgt gcataaaact ctttttttaa tgtttcttga cgttgttgtt
     8581 cttgttcatg attatcgaat gtattcatat acaaagcaac aaaacgattc ataaaggatt
     8641 caatttcagg tgtgtacgtt aattctgacc gactttgtac ttctttcttt tgtaattgtt
     8701 ggatttcatc tttcaaaaac gacacttgaa ccttcgtttt catggattga aaaaatgcca
     8761 agccccctga aaaacaaaga aataaaacca aacaccaaat ccatttcact gctttttttg
     8821 aatggatttt cttttgcttc ggtactttct ctgtataaac tttttctttc cgccgtagtt
     8881 gtcgtttttt cataagctac tccttctagt atgtttttac tagtacgtac gattgacaac
     8941 gataagcttg tttttctgaa aaatagtcca ttcaattacc ctcattttct attccttttc
     9001 tctttctttt atatccaaca aatccagcaa tacttgtcaa cactcctcct agaacagaaa
     9061 gtgcaacatg tatttcttca ccagtctttg gtaagacttt tgtttttcca tctgctgttt
     9121 tgatttcaat attgccactt ggaagtttct ttacaggaat cgacaaatcc tttaccaaat
     9181 tcccttcact atcttggatt aatggaatac catcttttac accaacaatt gtttgatcac
     9241 ctgctacttt taatggggtg gttggtgttg gaatatgggt caattcctct acattactag
     9301 tgacaatcgg tacactcatt ccttgagaag tattcgtcgt aatttcaccg cttggggtta
     9361 ccgatactgt gacttctttt gctggaacaa taggtttatt ttcttttgga attgcttctg
     9421 atttttcaac aggttgtttc ggttcttctt tttgttcttc actggtagac gacatcgttg
     9481 gctgttccgt acttgacgaa tcagtattct tttcagaaga actggttgtt cctgattgtt
     9541 ccggagttgt gactatactt gaatccgttg ttgttgaact atgcacacct tcttcttcga
     9601 tcgaatcagt cggtaaagtt gattcaggaa actctggttc tggtgtcact ggctcttttt
     9661 caggtttctc tgactgcaat tgttcaggtt ctgctggttt ttccgttgtt cctccattta
     9721 gctctgtctc aacatctggt aatccttccc ctggcaatgt ttctacaatt tctgacttag
     9781 gtgtttctac agtttcatct gcataaacca atgaactccc taattgaaac gaaaataatg
     9841 aaattcctag tgttgttacg actgctaatg ttgttttttt catgattatt cctccaattt
     9901 tttatgcaat tcgtgttttg ttcatagacg ccgcactaat attatctgaa cgataaaaga
     9961 actcccgtcc gttttcttct aacccaacat atggattatt tttgaataat tgaatcaata
    10021 gttcactggt gttgtatttt ggatagtttg caaaaaaata atactgtgct tgtcgcccat
    10081 ccgaaaattc aacaagtaat tcataacgat accgcttcaa tccttttcgt tgttttaaag
    10141 gatctctata ccaacttgtc catcgttcca ttcgtggata gagatagaca cgatacacat
    10201 agaacaatgc cataccacat accaccaaaa ataataatcg ccacataaaa attccccctt
    10261 ttcttctacc tttcttgaat catcgaacat tcataccctc tatagagatt tgaaaaatcc
    10321 atcatcaatt gttcatataa acgttgttgc aaatagcgat tgtttcgaat acataattct
    10381 tcatagccat caatcacttg atcttctttt tcatagcatc ttgtagctct atgacgctca
    10441 gcttgcatga cataaccaaa ctctgcacca tatcgttcta ctgttaataa ctgttgacta
    10501 cgcaataatg caatataaca atcacaaaaa tgttcaaccg acatttgatt tttctcacgt
    10561 ttgtgaaata ggtattcttt agaaagcaca ttattcgtta aacaatctga taaatagcga
    10621 taatctggcg gtcggttatt ttcatctgtg aatttttccc ataataaact gatttttatt
    10681 cgttcagcgg ctagatagtc ttgttcgcgt ggataaaatt catcaatcgc acaaagtttt
    10741 ttatcacctg aataaacaaa tccatataat gcgaaacgaa ctcttccttt tcaattggct
    10801 ctcctaaaaa ttccagtgac ataccatctt ctccttccta aaaaaaataa aaaagcagaa
    10861 accgatttgg cttctacttt tctttccttt tatagctctt ttttacggaa gaatccgcaa
    10921 atgacatttt ttcaaaaaat caatcggcac aagttcacga aaacttggag caattttgga
    10981 tagaatcact tccccatctt gtaaaataca atcataaaaa tcatcagtta cttgatactt
    11041 acgcttctta caataattga taaaatagta gcgatcattt tctatttcca ttcgttgttc
    11101 tactaagcgc atacgaacat catcatagaa ataagccaca aatcgccctt caagttcatg
    11161 tttttcaatg ggatactctt ctggtaacaa tctatttttt cgtcgtttat acattgctgg
    11221 ataccttaga agaagttcat ctaaacgtct gtcagtatat ggtaactgac gaatcaacga
    11281 ttctaatcgt tctttataca gatagaataa ttggcgcaag caatagtgta ccgcctctgg
    11341 attttctaat actgttggat cacgtgacca atacgcacca acataaatca taattttttt
    11401 catttagaca atcacaccag caatactaat gaccgatcca caataggtag aacaagtggc
    11461 atagcgacca atttcctcta attctatctc aatctcttct aaagaaagat atgtctgttc
    11521 ttctgttggc tggcgactca tttcccaagt gacagtcacc aatgaaattt cttccataag
    11581 aggtttttcg ctgctaagtg ctgccacaca gttttcatat atcttcattt tatcgcagcc
    11641 ttttttaacc gctaaaatcg caggtattgg caatgaaaat cgataaaagc gaatgaattc
    11701 tagccttcct ttccttcggc taaattccaa ctcttcgagt tcttgttcgt atcccttacg
    11761 aacttctaat aagcgtgcaa tttcttgcca gttctctttt tcctgataac gtccagcttt
    11821 taccgcttgt tcaatataag gaactcgaaa acgaaaataa gacaaatcaa ccatcttttc
    11881 atttaatgct tcgattgcag cctttaaatc caacaggcgc agccattctg ttgtcgtata
    11941 tgcattcatg caattttccc tcctgacgtt cccatccatt ctagatagtt ttgttgctcg
    12001 tcatttagtt cggcattatc aatcccttct tgtacaaagt ctcttcctaa aatacgatct
    12061 gcttcttgta acaactttaa tgtcggagct acttgtttca aaaaccagcg ttctgaacga
    12121 tctaatgaga atttttctgg ctttactgta aaatccaatt cttccatcga acgaaataat
    12181 tttgcccatc gtttatcaac aatccgtgca tcactttcca gtctatccca aacaataagt
    12241 tttgcattaa ttaaaccaac gccaatctct cctaaacgtt tcggtgaatc acctaaaata
    12301 aatgcttcta ctgcttgtgt tgctttctca ttggctaacc gtaactcata tcgattataa
    12361 ataccatatt tcgttaatgc ttctaattct gaaatttttt ctcggttcgc aatttcaaaa
    12421 cgtttctcat aaaaattaaa aaagagagga ctctttcgcg atccaaaata caaagataat
    12481 ccttcacttc gaaaatagcc atcatctaaa aaacaaccgc caatattctg gaatctctta
    12541 aagtccattt ctaataaacc tgctttttgt ttttctagta attcaaagag atcgaaatgt
    12601 tcttccgttt ccaatcccca aagttcatct aacgcaatat cgaatcggct acattgcaca
    12661 cgtgtataac cagcttccaa ctttccttga tttttccaaa tggaatatag ccatgttcgc
    12721 caatctgttt gttgttcacg catgacttcc tcatattccc ggcaaccttg tcctgaaaat
    12781 tgcacaagta cattttctgt tacagttcgt tttgaatgcc agaacaccca gatattccca
    12841 cgaacagaaa gacagtcaaa aagatacaag cccgttggac gtgtttcaaa ttctgtcaga
    12901 tccattcgta atacacgttg aatcactgtt ttgtagtctg tcccaaaaaa ggaaagacgc
    12961 aaataatcaa tcattgctcg aagatctatt ccgcttttca tatacaatcg aaagcgttcc
    13021 ttgattcgtt gttcaaaatc ctctgggatt tttcttttcc cttgttccat ctttctaatg
    13081 taatcttttg agcagcccat caattgcgca aatcttgctt gtgatagcgg ctttttatct
    13141 ccacttatat tatgaattgc acttaacttc tttctgtagc tttttagctc aatcggtgtc
    13201 attttctcgc tccttttata aaataacaaa atcgattttt tttaaccctt tagaaacacc
    13261 gatatatcaa ctagtccccc tcgttttttg ggaaaagtag tacaactgct gaccttagat
    13321 aatcacttga tacaaaaggg tttataaaac ttttttctat gtgttatgtt atttctgccc
    13381 cccatattat atatcggggg gctttacaca aagaggccgt gcgcaagcgc acgacctctt
    13441 tgtgagctgc acacgctgct agcgagtgcg cattataggc ccatgcttcg cagccttaat
    13501 gcgcaccgcc aggcagagtg cggctaccag actacgtcta gagccctccc gatagataat
    13561 aactacaaaa ttaaatgatc taattttgtt atttcactat atttttcagg agaaaaaacg
    13621 cgataaattg acaaagttaa atcctcttct aaatctactc gataatgata caatcgagtc
    13681 aatcgttcta tatctttttt cgttagcaat acttgattaa taacttttcc tgtaaaaaca
    13741 cgttctttgc gcaaaaagcg gtaatcagtt acttgttcaa tacgattcaa ccaatcataa
    13801 aacgttctgg aagaaagaga aggattaatc attcgtattg ctgtagctac tctataataa
    13861 caactctctg ccatttaact tcacctcctt agttgattac aaaatatgcg catcagaaaa
    13921 acttgttcct tttggatctt ttgctaaata catttggcgc tctagttgtt cttgtaacaa
    13981 ctgattatca tctcccatcg tttgatcttg actatcctct tttactgtaa aatcatagac
    14041 aatcatttca gtaaatttct caaattcttt gataaagtga taatctttgt aaacaaatgg
    14101 cgcaaaaaac tctacaggta catcaaatcc tggaaaagca tatccacgac caggttgatc
    14161 tttgttgtaa aatggcttat ttttaaaatc ttctccaaaa gtcattaaat aaccaatggg
    14221 agacaatcga ccaacagaaa tacggaatgt taaattatcc cgaacaccac ttggaaaata
    14281 ttctgcatct ggtcgttgca ttgctaaaat aatgtacatc cctgcttgac gaccctttaa
    14341 tactaatggc tgaatcaatt tatcgactct atttctcgct tccatatcca acgaaccata
    14401 aaaagctgcc cattcatcaa aaaagataaa atgcggtggt aacccataat aagcataatt
    14461 tttccctggt tcatatttag gatctttcat ggttttcata taggcatacc gttgattcat
    14521 ttcttttaat gcatttcgta aacaaataat catttcttca tttattttgt aaaacacatg
    14581 tcctttgaat acatcgacgt cacctaatgc tgctaaatct gccgcttttc catcgcaaat
    14641 atctactgtc ccaacactta aacaagcttg aattaaagaa tacaataaat aggttttccc
    14701 accaccgatt gcaccagcaa tcaacatatt tggtgattga tcatataccc aatccaaagt
    14761 tttcattaaa tgaatacgtc cattttctgc tatacattga tcaataggaa tccgaccaga
    14821 atctgaaagg aatacataat tgattcgggc tttttctcgt tcttcacgaa ttaaatcacg
    14881 caaaaatact tcagataaca aatgacccat ttttaaaaat cgttcgtgaa aacgcccacc
    14941 atctaaagga aaagaaacca ctaattcttg tttcttttga taaaaataaa cttttggtaa
    15001 tctcattttt tcccttgttt gtttctcact aacttgtttc tttgtataaa caaaccgatt
    15061 gtcaattaac attcgaacaa gtaactgacg ccaataaaca gaggcaaaat atccttcttt
    15121 tagtcgcgac caatgcatca accctaaaat taacccacta accagcaaca aatacaacac
    15181 aatacttatt ccaggaataa gatacatttg ccaattcgaa aacgtactta aacgtggata
    15241 aaatactttc caactaaacc aacctaacag aatcaaaaat ggaaacaaaa agaaaccata
    15301 atagcaaaac cataaataac gattgaaata gcgaattcgc cgaccacgat acttgaaaaa
    15361 atgagctttc aattgtcaca ttcctccttt ctgtttttac ttttcctgtg ctaaaaactt
    15421 ttgacaaaaa gcaactaata caggaatatc acatttagta aaaaaacata atcgaaaaag
    15481 atttaatcga tttttataag ggtgtgcaca catattacat attcgtacta tacgaatcca
    15541 aatcacttcc tgatgatctt gtttccactg gtacggtaat gaaaaacgaa caagatcacg
    15601 taaaaacata cactgcccaa taaccgcaca aatatatgca tttgtttgta caaaaatcgt
    15661 tggattgtct ccaaaatact caatcaattg ttggatattt tgataacaag cacaatctct
    15721 caaagtattt gattggtttt atctgaattc aaaatctccc cttccttcta tcttagataa
    15781 aaagaatagg aagactcctt cctattcttt caaaaaaacc ttattttttc tctttttcct
    15841 tagaatcaac acttggttgt ttttgatcta aacctttttc ttgtgattgt ttcatattag
    15901 gattatttgt tggagcttct actttctcta atcctttcgc ataaatcact aaatctgaat
    15961 attcacgatt gccatcttgt gctggaatat ataccacatg atattctgga tcaattaatt
    16021 tcacaggatc acgtcgttta aaaccagtca aatctacatc tgctggaaat ttaactcgtc
    16081 gtgcaatctt tggttcatct ggatttccaa aaactaattg tgctcgctgt gatcgaattt
    16141 ctcccgtata atcgcctgtt tcattatcaa atacacgatc agactcttct aatgcgccaa
    16201 agcgtacggc tcctaaacaa gtttcatcta atgcttctgt aatgtcataa ttatttggaa
    16261 attttaatcc catcttacaa aacctcccca atttttacac cagttaaaaa gacaccaaca
    16321 tcttgatagc cagttgttcg acgctctgtt tgtcccatat catttgtttc acgactattc
    16381 actcgttctt tacgaaaaac aaaataagct tcttcaaatg aaatacgcac ctctttttct
    16441 ggtaatcgta atcttttatc ctcttcagct gtgagataaa caaaaaaagc aggctgttct
    16501 tctcgcgaac actctgcttg taattgaaca tatcgcccag acacaccatt aatttccttt
    16561 ggtggattag gttcccgtga acccaataaa aatacttgac ctaaaaactc tttcttcata
    16621 cgcaatgctt tttctgccat aaagtttttc ctcctcttaa aaaccaaaat aaattctaaa
    16681 tatttctaca ttacaatcaa acaagcggtt aaaaatccta accgcttgtt cctcatgact
    16741 aatctttggc tttacgtact ttataaagtg ttatacctaa agcaatgact aatactacta
    16801 atccaagaac cataagtata ctacttgttt gttcacctgt ttttggcaac attggtacaa
    16861 cagtagctgg tttaacccgt tgttctggtt tcactggttg cggtggtttt ggcgcttcca
    16921 caatttgaac cgtttgatct ttgtttgtta gatctacatt gtgtttcgcc acttcttcaa
    16981 acgtttcttt ctcatcttcc gttttcgctt ggagccattc cgcaaaactc aagccttttc
    17041 ctgcaagatt gcgaccgtcc actaagaatt caaataagaa ttccatcatt tctttatcgg
    17101 ccgcaaagac tttttccgca tgagtgcctt caaatggtgt gccgtctggt gttaagtagc
    17161 cttgaacaat taagcgatag atttgaccaa tcgcaagacc gtctactttg acccattcaa
    17221 gcacagtttg atttggcaag gccaagatct cttttccgcc atttttattc gtcgctttcg
    17281 tcgtaatggt tggattactg attttgatgg tttggtcatt gtcggtaata tctgcgtgag
    17341 aagccaataa tacgccttct ctaaacagat cttcaaagac cacgacttct ttgcctcgca
    17401 acgcacgtgc gtctagaata aagtctagat tgactgtacc atccgttgtt tctgccacaa
    17461 acgttaaggt acttgtgact tcttttccat ctactagcac tggtttttcc gttactttgt
    17521 ccattagaac accgttgact gtgtatgttt ttccaacgac caaatcgtgg tatttcacag
    17581 cgtctgtaat ggtgactttc gttaatggat taacctcttt caagccagtg ggtttatgtg
    17641 tagccgttgt acgtacactt gggttagtta ctcgtaccgt ttgccctttg tcgttgatgt
    17701 ctttatggtt tgctagttct tcatcatttc taaaaagact ttcaaacacg acgatttcac
    17761 gaccatttat acctgtcaaa tcaaaggtaa atggtacgat caccgtttca ttttctgctt
    17821 tggcaataaa gccaacactc gtttctaccg tatttccatg cacaagcaag ggttcaccgg
    17881 ttgctttatc cattaagatc cctttgacca agtatgcttt tcctggaaga atctcggtaa
    17941 tttcaacggt atcgtttaag gtaaacaatc ctgttgggtc tgcgactgtc aatccgtctt
    18001 caaagacagc cgtggtatga acggttggat tattcacttt cacactttgg gcttcatcgt
    18061 tgatatccgc atgtgctgca acttcaatgc catcacggaa aagtttttca aaaacaacga
    18121 tcgtttgacc tttcagaaca ctagcattaa ccacaaattc aaggtctttt gtgccattga
    18181 ctttgtctgc aataaaggcc aaggtacttg tgatttcttt gccatcttgt aaaattggcg
    18241 cattcgttgc cttgttcatc aatactcctg aaatggtgta ttcttttccg acaaacaagt
    18301 tggtataaga aacactgtct ttaatgacga tcgtttctaa tggatcaacc actttttcac
    18361 catcggcttt atttgacgct tgtgtttcta gttttggtgg aatcaagtaa ttacgtacag
    18421 aaaccactaa cgtttcccca tcatctggcg cattttgttt gaatgcaacc gtgaatgctt
    18481 gtttatctgt atttggttgg aaactttcta atggggcatt ttccaagaag taatagtcat
    18541 cggcaactag acctttcact aaaatttccc cgttttgatc agtggtatat tcaccaattg
    18601 cttgattttt accgttatac aaggtaaagt tcacacctgc gagcggcgtt ttcttcttgt
    18661 tccattcttc atacttgaat aattttacac ttttccgtgc ttgacggttc gcaaaatgga
    18721 tcactttgat cggttctgta tcattggtaa tcgagaacac taatgggttt tgattcaaaa
    18781 cgtaatttac gggggctttg atttcttcca aacgatagcg atcttttcca taagtaagtg
    18841 taccgttagt taggaaaaag cctttttcat tggttttaaa gatatccgtt gtttctgtat
    18901 catttggaat ctccattgtg acataacgat tggctaaagt atcaaaaatc ttgaattctg
    18961 tattagcaag tggaatcact tttcctgttt cttgatccac tttcaccact ttaagacgtt
    19021 gttcaattac tttatcttct aaaacataat ggaaagtttg cccattttca gaaattgtga
    19081 cttcaagtgg ttcaaatagt ttataccctt cttttccttt ggtttcagaa agaatgtacg
    19141 tatcaaagac aacggtattt ttaccaaaag tagcataacc atcattgtct gtcacaacgg
    19201 ttttcaccac tttgccagtt gtttttgacg tcaaagtgat ttcaatgcca gctaattttg
    19261 gtttgttatc tggattcgtt gaatcgacta aatctttatt agcaactttc actaaatcaa
    19321 tggttccttt gatcacttga tcttttgcgg tcgcattgac agtttttcca ggttcaacaa
    19381 caatttcaat tgcttcatca gaaagcacat aaccttttgg cgctttgatt tcttttaaaa
    19441 catatttgcc aggatcaaga tctttaattt ctgcctgtac tttgccgtta atttttttca
    19501 aggtaatttc tttaatcttt ttcccatctg cttgatacaa accatagacc gcattttcta
    19561 aagaagctgc accttgtgct ttcacaccag tttccgcatc ttctttaaac acacgaatgt
    19621 ttccttgttt tagaatttgt aaattaataa cagacgcatt aggttctgtt ggatgtaaca
    19681 caccaattgt ttgagctcct ggacgacgat aggctaatgg cgcaccttga actttaattt
    19741 tattcaattg aatttttgct ttatccggtg cctcttttgt gactgtaatc catacttcat
    19801 tgccgtgaat agatacattg acaccttttg gtttttgaat gtctttcaca tatttcaaaa
    19861 aatcattttt attctttact ttcaacgttt cgccaatttt taccgtatgt gtttgattat
    19921 taaaagttgc tttttcacca aagttattaa tcaaacgttg aatttctgct tttcgacgat
    19981 cataatcaac acggatattt ttgattggga tttttgtgcc caaagcttcc caaatcataa
    20041 attgtgccgc aatgtattgt tcatcggatt tgtctttatt tgtgacatat cctaaataag
    20101 agatcaagct tgtttttttc cgtacatttg aatttttcaa atagtttttc aaagcagtag
    20161 ctgtataaac ttgaccttct gcggctgaga caccaggctc aatacaaaac gccacttgtc
    20221 cattaatctc aaaacgaacc atataattta aagtgaagtt tagattgttc ggactagaag
    20281 taacaccaat attttcgccc caatcaattt tccgaccacc taaaacagct gcattggttg
    20341 cacggcgaac acgaaccgaa cgattggttg gtactgttac taacagtgta ggtgtacctg
    20401 tttttggaat gttctgctct tttttctttt gattttcaat actagccgta gattctttgt
    20461 ttggtttcgt tggttgattt tgactagctg cggttgaaga gattgttttt tcaacgtttt
    20521 cctcattttt tccttctttg tcagcagtag ccgctggttg ttccactgtt tcttctattc
    20581 gtttcttttc ttccgcttgt ttctcagaag actctggctg cacagaaggc gttgcttcgg
    20641 cagctttttc tccttctaga gtggacgttt ctgaagcagg ggttgtaatt gaggcagttt
    20701 cttcaactgg ttgtgaagta ccaggactat tccctgggtg ttgtgtttcc ccaatcgtct
    20761 gatcgagagt cgttttggaa ttgaccgttg gcgcttctgt cacaggggcc tgttcaacag
    20821 tggcttcacc agctgggacc ccggcttctg ctggttggac ttctgtcact tcagaagcga
    20881 cttgggcaat ggcttccatc gtaggttgta tagccgtcgt ttcttctgca tgtgcttgag
    20941 cagttcccac caagaaacag aattgagaaa aagccaccaa tgcgagtgct ttcaaccaat
    21001 tcattttcgt ttttgcatac acaagaattc ctccttttcc tgagaatttt atataataaa
    21061 acaaaacgcc gttaccaaaa gtgataacga cgtgaactgt ctcttctatt tttatctggc
    21121 gctcttatct gaacacattt catcaatgat tttaccctcc cactataatt acagaacaaa
    21181 aaaagaacct gcttcgtgga aatcaactgt ccatttccca attggaatac cagctttttt
    21241 ggcaacttca ccatcaccaa atctcatgcc ataccaatta ctaccttcat ccttaatttc
    21301 tttccaacca tatgacatag cttcatctaa tgaatcaaaa atcatgacac tattgccaat
    21361 tgttacagga tctggcttaa gatcttctgg aagagaaata tcaatcatac cataatgatc
    21421 cgattcctca gtattattgt cattactagc tactggtttg gttgttctac tggcttattt
    21481 tgatacaaca agtactctta ttcttctttt acttctccgt tttcaatttc ccattcaaac
    21541 cactccgcaa agtcttgctt aaaaccatct actcgatacc tattttgtaa ataggcatct
    21601 tctgacgtcg taggatctaa ataggtaggt ttattctctg gacgagcata gacacctacc
    21661 catgtttcgt aatctccttc aaaataacta tctaccaaat agcctttttt tctaagaatc
    21721 tcccgaactt gatttttaat tattgatact tgttcaaaac catcactcat tctatcacct
    21781 caaataactc tagattctca taaatattcc cgacaatttc aatcctatca atgatctcta
    21841 acaaatcgct acttatgctt ccccattcgt ataagaattt cccttccgca aaataaattt
    21901 tccataaact tcttcatgat cgtcccaacc aatatcactc tcgaaaaatt ccacgttgtt
    21961 cttatctttt agctcagtaa atgccataag ttcaacatgt tttccaacct cgaaacaatc
    22021 acaccaccca tcaaccgtta cacgtgtgcc ttcaatacct aagaaacttt gtcctcgtgt
    22081 attaaacact aaaactttcc gcattttttg ctcattaatt aatcacgcgc gaaatctagg
    22141 aatcacaact tattcgcttc ttctttataa atcgccgatt gtaaaattaa gctagacaac
    22201 taaaaaaagt catttgtgct acactcaaaa tagttccgac gaaagaatta taaggagtga
    22261 gtacaaatga cctataacca tcttacacca actgaactcg taatgataga agcttatttc
    22321 aatcaatcac aacctgtttc aaaagtagcc aatctgttac aacgttctcg acaaactatt
    22381 tataaggtct atcgttttct taaatctggt ggaagcgcaa tgagctacta taaacaatat
    22441 aagaaaaaca aacgtaactg cggaagacat ccgattgttt tacctgatga gcaacaggaa
    22501 tacattcaaa aaaaggttgt tcaagggtgg gctcctgatg tccttattgg tcgagcagag
    22561 tttcctattg cttgttcggt acgcacactg tatcgaatgt ttaaagataa aacctttgac
    22621 actcacgatt taccaatgaa gggaaaaaga aaaccaaacg gtcataaaga aaaaagagga
    22681 aaacaagggt ttcgccggtc aatccgagac agaaagacag actataccca gtttgatcat
    22741 gaatttggtc acctggaagg tgacactatc gtgggtggaa aacataaaag cgcggtcatc
    22801 acattagttg aacggctatc taaagtcatc attaccttga aacccgaagg cagacaggct
    22861 aaagacattg aaaataggct gaatcaatgg tttcgttgtg tcccaaaaaa cttatttaaa
    22921 tccatcacat ttgattgtgg aaaagaattc tcaaattgga aaaagattag taataccaac
    22981 gatatttcaa tttattttgc ggatccagga acaccttctc aacgaggatt gaatgaacat
    23041 tcaaatggct tattacgtaa agatggtttg tataaagcta tggattttaa tggtgtatct
    23101 gaaaccttta tccaatcggt ggcatcgaaa cgaaatcata ttcctagaaa atcattacac
    23161 tatcggacac caatggaagt atttttgagt tacgtcacaa aaga
//
LOCUS       DAZKLA010000033        22897 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000033 DAZKLA010000000
VERSION     DAZKLA010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.033"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            166..330
                     /locus_tag="ACG85N_001106"
     CDS             166..330
                     /locus_tag="ACG85N_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223892.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            327..557
                     /locus_tag="ACG85N_001107"
     CDS             327..557
                     /locus_tag="ACG85N_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223893.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQQNIERWIDR"
     gene            742..1287
                     /locus_tag="ACG85N_001108"
     CDS             742..1287
                     /locus_tag="ACG85N_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0223894.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            1384..1605
                     /locus_tag="ACG85N_001109"
     CDS             1384..1605
                     /locus_tag="ACG85N_001109"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFQ0223895.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(1762..1926)
                     /locus_tag="ACG85N_001110"
     CDS             complement(1762..1926)
                     /locus_tag="ACG85N_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFQ0223896.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            2028..2549
                     /locus_tag="ACG85N_001111"
     CDS             2028..2549
                     /locus_tag="ACG85N_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFQ0223897.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            2536..2976
                     /locus_tag="ACG85N_001112"
                     /pseudo
     CDS             2536..2976
                     /locus_tag="ACG85N_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(3016..3174)
                     /locus_tag="ACG85N_001113"
     CDS             complement(3016..3174)
                     /locus_tag="ACG85N_001113"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFQ0223898.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            3267..4028
                     /locus_tag="ACG85N_001114"
     CDS             3267..4028
                     /locus_tag="ACG85N_001114"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0223899.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            4215..5567
                     /locus_tag="ACG85N_001115"
                     /pseudo
     CDS             4215..5567
                     /locus_tag="ACG85N_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            5583..6803
                     /locus_tag="ACG85N_001116"
     CDS             5583..6803
                     /locus_tag="ACG85N_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0223900.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            6838..7515
                     /locus_tag="ACG85N_001117"
     CDS             6838..7515
                     /locus_tag="ACG85N_001117"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFQ0223901.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            7512..8705
                     /locus_tag="ACG85N_001118"
     CDS             7512..8705
                     /locus_tag="ACG85N_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0223902.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            8720..9046
                     /locus_tag="ACG85N_001119"
     CDS             8720..9046
                     /locus_tag="ACG85N_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0223903.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            9050..9385
                     /locus_tag="ACG85N_001120"
     CDS             9050..9385
                     /locus_tag="ACG85N_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0223904.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            9382..9777
                     /locus_tag="ACG85N_001121"
     CDS             9382..9777
                     /locus_tag="ACG85N_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFQ0223905.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9774..10145
                     /locus_tag="ACG85N_001122"
     CDS             9774..10145
                     /locus_tag="ACG85N_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223906.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            10161..10721
                     /locus_tag="ACG85N_001123"
     CDS             10161..10721
                     /locus_tag="ACG85N_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFQ0223907.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            10846..11157
                     /locus_tag="ACG85N_001124"
     CDS             10846..11157
                     /locus_tag="ACG85N_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223908.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            11181..11372
                     /locus_tag="ACG85N_001125"
     CDS             11181..11372
                     /locus_tag="ACG85N_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223909.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            11372..15220
                     /locus_tag="ACG85N_001126"
     CDS             11372..15220
                     /locus_tag="ACG85N_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFQ0223910.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            15221..16093
                     /locus_tag="ACG85N_001127"
     CDS             15221..16093
                     /locus_tag="ACG85N_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFQ0223911.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            16090..17139
                     /locus_tag="ACG85N_001128"
     CDS             16090..17139
                     /locus_tag="ACG85N_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223912.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            17143..17967
                     /locus_tag="ACG85N_001129"
     CDS             17143..17967
                     /locus_tag="ACG85N_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223913.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            17985..20045
                     /locus_tag="ACG85N_001130"
     CDS             17985..20045
                     /locus_tag="ACG85N_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFQ0223914.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            20062..20211
                     /locus_tag="ACG85N_001131"
     CDS             20062..20211
                     /locus_tag="ACG85N_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223915.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            20208..20654
                     /locus_tag="ACG85N_001132"
     CDS             20208..20654
                     /locus_tag="ACG85N_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223916.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            20656..20793
                     /locus_tag="ACG85N_001133"
     CDS             20656..20793
                     /locus_tag="ACG85N_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFQ0223917.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            20828..21070
                     /locus_tag="ACG85N_001134"
     CDS             20828..21070
                     /locus_tag="ACG85N_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFQ0223918.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            21086..21283
                     /locus_tag="ACG85N_001135"
     CDS             21086..21283
                     /locus_tag="ACG85N_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0223919.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            21294..22310
                     /locus_tag="ACG85N_001136"
     CDS             21294..22310
                     /locus_tag="ACG85N_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0223920.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANP
                     TLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
     gene            22425..>22897
                     /locus_tag="ACG85N_001137"
     CDS             22425..>22897
                     /locus_tag="ACG85N_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223921.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQT"
BASE COUNT         7967 a         3893 c         5071 g         5966 t
ORIGIN      
        1 caatgttcga ttcattgcca gtgactttgg caaccgaggc atcggcggtt taaaaatata
       61 ggggtgcgca atttcgtacg cgttttgtgc atcgtgtagg ttgctattac atattagatc
      121 actcgttgag tggtcttttt attttacata aaggaggtaa caacgatgta tagaccacaa
      181 tacttagaac agaagtatga agtaatcact gtgcaaaacg gtaacggtga gatagtacga
      241 aagtatagaa gaccaataaa gagcgataca tataaacgaa aggaaaacaa tgaagttatt
      301 tcttttcgca gaaggagaaa agccaaatga gaaactactg gtatgtatcg ctaactaatg
      361 aatatcctcg aaccattgat gattgttcag tgcgtgttgt gcgttctgta caaatcaaag
      421 ggaagtactc tattgtcgaa atgctaagag aagctacacc aaacgaagtg gataaatgca
      481 agctgatata ttgcggtcat ggctattgga aagacgagta tatccaacaa aacattgaga
      541 ggtggataga tagatgagtt acctcgaaca tttgaaacgt tgctacatgc attctaagaa
      601 taaacttcct gacagctaca ctgtagatga tgttgctata catgtattga agactgagag
      661 ccatagcagt cctgatggta gcagtaagga gcagacgctc gcgtggttca agttctttaa
      721 gtggattaag gaggaagagt aatgcacatg cacataggag aaaagatata taaattgaga
      781 cggcttaata acgaaacaat gaacgactta gttaaacaaa ctggagtgtc aaaatcatgg
      841 ttatctgata ttgagtctgg aaaaaagcaa cgcgttgatt ttaataagat atgtgcaata
      901 gccaaacatt ataaagttag cttagaatac tttcgggatg attatttgga tagtgatgaa
      961 cacgaggata ttattccttt gacaggcgaa acaagagtta aatcagtgaa atcgcttgaa
     1021 caaagcctaa agtatctgaa taacaaacaa gaacctaacc ttctagagat caaactaaaa
     1081 gatactgact cagtaccaga ggtttggtac aagggtgaga ggttggatga attgcctaaa
     1141 ggattagtag atgtctcgta tcattggaag actgatgatt ttactaatga tgatagagga
     1201 gcgaacgaca tcacgattca atacttttct agctttaatg ataagtatcc agataagaaa
     1261 acaatcggac acaagagaga tatgtaaatg aaagaagtta gacctagaga cgaaatagac
     1321 aaactataca agaccaaacg atggcgagac ctaaggcaag tagtaatagc tagggacttc
     1381 ggcatgtgcc aagagtgcaa gcgtcgaggg cggaacacaa ggggcacgat catccatcac
     1441 atagtcgagg cgagggaaga cctgtcactg ttctggtccg tagataacct tgaatgtatc
     1501 tgtgtagctt gtcacaacag agagcatcca gagagatcag gcgggaagaa gaaaccaaaa
     1561 cctaaatcac atatcgttaa aatgtattca actcctgaaa gataagtttg cagcgaaatg
     1621 aaggtagccc ccctactcta aaagattaaa gagtaaggct tgagaagaac ggtgctgtcc
     1681 ttccttcgta aaaagaccgc ttttcaagtt ttttggagaa aaaggaaaaa gccgaccaat
     1741 ttaagccggc tttggacgaa gctatttctt agtccatttg tttccttttt gagaagtagg
     1801 gggtaatcgg tcgcctggat caatttttac ttcttgtccg ccttggacat ttccaccacg
     1861 aggacccact tctttgtagg ttccttttgg tttgttgtct tcgccgggtt tatagagttc
     1921 tcccatagga atccctcctt aacaaatttc ggcacagcac tgccgataac ctaattataa
     1981 ggattgtgat aacgatttca atctatcttt tgaaagaagg tgatattatg ccgcaaccag
     2041 cgaagagtgc aaaattacaa ttattaaacg gaaacccaaa taagaagaat accgaagaac
     2101 tccgcaagcg agcggccgca gaagacaaat taaaaatggc tactgacaaa atcaaaccgc
     2161 cgttatggct agattcgcta ggaaaggata cctttgagtt tatcgccgat gaattgctgt
     2221 ctgtggattt aatcagtaat ccggacgtcc atacaatggc tctatactcc aattggtatt
     2281 cgcaatacgt ttctttagaa aaacagcttc gaaaactaca acgagagtac aagttgaact
     2341 atgcgcttgc gaaaaaggag gcagaggcga gaggtgagcc gtttaatgaa cctaatgaat
     2401 taattggtaa tccgctctct cggcagatgg atacagcgtc tcggaatctc cgttcttttg
     2461 gcgctgattt aggactatca ccagcagcca gagctaagtt agctattaag atggctgatg
     2521 atggtggtga tgacgatgac gacttctaat attttggata tgtcttacac agaacgtgtg
     2581 gactattggc aaagctatct tgaggagcaa gcttcttggg gtggcttttt aaaatgtcca
     2641 tatccggaat tgttaactac ttggtatgcg gaacgattaa tcgatggaag cataccagcc
     2701 agcaaagaaa atattcaagc tgctaaacgg catatgcgtg atttgcagcg ccaaggaaca
     2761 gatgattttc cttggatctt tgacgaagaa aaaggtcacc ggcctattag atatatcgaa
     2821 aaaaaatgta aaccaactga aggcgacttt ggttcgtttg ttttgcaacc ttggcagcat
     2881 ttcataattg gatccatgta cggatgggta catcgtgata caggagagcg tcgcttccgc
     2941 gaggctctta tttttgttgg acgtaaaaac ggtaagtttt gccgtttaaa aatcgggcaa
     3001 aatcggtgaa aacctttatt tttggctttc ttttagtaat aattcgatag ctttatcaag
     3061 caatttagac ataggaacca tagtttcttc cgacattttt tttaatcctt cgtataaatc
     3121 tttatcaatt gcgttagaaa ttctaattct gttttttagt ccgtatttgt ccatgtgtga
     3181 cactcctttt tattttacta taacatccga ttccaccgct tgcaactacc atcaattgat
     3241 ggtagtatat aaaagaggtg agtagattga aaaaatacat tatttacaaa cacttaaata
     3301 aaataaatgg caaaatttat atcggtgtca caaatgatat tggcagaagg tggaggagcg
     3361 gtgggattga atataaacca ccaaagaacg aaacccaaca ccacagaagt ttttggaatg
     3421 caatacaaaa atacgggtgg gaaaattttg atcatttgat tattgaagaa gacttaacga
     3481 tgaaagaagc ttttgagaaa gaaaagtttt atattgaact ttatgactca actaataaga
     3541 aaaaaggtta taacatcgca aaaggcggaa atggcggaat aatatataaa gttcacccca
     3601 aaggaatgct agggaaaaaa caatctaaag aattttcttc aaatcacagt aaatgggcaa
     3661 agaaccataa gaataattgt atgacaaacg gtgatgttgt atggggtgtt actcacgaac
     3721 acccaaaagg catgttggga aagcaccaca gcaaagaatc aattatgaaa aagaaagcat
     3781 atagtggtga aaatgctgta acctcaaaac caattgtcgc aattgaacgg gatggaacta
     3841 aaagagaatt tcacagcgct aagctttgca tggcttacta ttcgattagt acatgtgttt
     3901 tttaccgact tttaaaagat ggcgcccctt atgtgataaa tcctaaagcg aactacagaa
     3961 ataaagaaaa aatattagct atcgaaggaa ttatgttcaa acataaagaa gataccgagg
     4021 taagttagta gattgcgaaa ggttactaac taccgtaacg cgtaggagtt gaataaatat
     4081 aatgctccca agagtgtccg acagcagata atttgctgaa aatgtacgct aaactgggcc
     4141 agaactgact gaccgatgaa aatgagggtg acctccagag tgcgagataa aaagctcgca
     4201 gataataaca atcgaaaaca tcactaattt caggcctttc cacatacatg gtcgcttatg
     4261 atgatgaaca aggcgccaac gtttacgtat tggcaaatgc tcgtgatcaa gcaagcttgt
     4321 tgtttgataa ggccgcagaa atggtcaaac aatcgccggc gctctttaag aaatttggta
     4381 agcctaaacg atcaagtatt aattatgctc ccgccttttc taaaatggaa ccacgcgcct
     4441 cagatagccg gaaattggat gggctaaaca ctcactttgg tatttttgac gagatccacg
     4501 agtttacgaa ttacaatctg atcaacgtta tcaagaaatc aagaggaacc agaaaacagc
     4561 ctctgatagt ttatatcaca actgctggat atgtattaga tggtccgttg atgtcttatt
     4621 ttgagcaagg tgtggactgt ttggaacatt tggaagatga catcgatgaa cggactttct
     4681 attatctggc aaaacttgac agtgcggaag aggctgatga cccaagatta tggatcaaag
     4741 ccaatccgaa tatttgtcta atgaattttg ttggcatgct agatgactat gttaaggata
     4801 aaaaagatcc aaaagaatat gctgactgga ttaccaagca atttaacttg ttttccgata
     4861 tcgatgagct gtcatttgtc gatatgccta ccattaaacg aaacaataaa accatcgata
     4921 ttgaaacgct caaaggtaag aagtgtgtcg gtggttttga cttgtccgaa acggaagact
     4981 ttaccgcagc cgttttagaa tttccgcttg aaacaggcga ggtattcatt ttgcaacaca
     5041 catggatccc acaagctaga tttgatcgag ataacaatca agagcgtatc aaagcgtggg
     5101 agaaggtggg agatctaacg attattcctg gtgattacgt caattatgaa tacgtcttaa
     5161 attggtttgt agaaaattcg aaaatctatg acattgtaaa aatcaattat gacaaggcca
     5221 aggcgctacg attaaataaa gaactagaaa atgcaggatt tgaaaccaac gagattcggc
     5281 aagggtttct atcattaggt gggccaatgc aaaacttcaa ggaaatgcta ttggacggta
     5341 aggtgatttt caacaattcc aagctttacc gatggtatct atccaacgtc aagctggtga
     5401 tggatcgcaa ctcaaactgg atgccgtcta agcagtccaa gagtagaaaa atagatggtt
     5461 ttgcagcaag tttgaacagc cacgccgaag tgttgaatat gttggttaat cctgtcggaa
     5521 ccgggaaagt aacctattac tcgatttccg atttaatgaa tatgtaagaa aggtgtggag
     5581 gaatgagtat tttagatcgt ttgcgttctt ttggccgagc gaagccgaaa gcgagcaaac
     5641 aagagtattt tttgaatgac ccgggattga taccgtattt agtcggaaaa gatgaaatat
     5701 cagaagggat tttttccgta attagccgtg tatcgaacgt ttttgcgtct ctccctctca
     5761 aaatgataga tgtggagttt ggccaaccgg acgactgtcc tgcatacaac ttgttgagcg
     5821 aaggccctcg atattttaca aagtttgatt ttttccggga cgtggaagtt ttgagaaact
     5881 accaagggaa tgcgtatgtg cagattttcc gaaatatcaa tggagaagta gcagatatgg
     5941 cgttagtaaa acctggtgct tgccatccag tgattgatat ggatagcggg gagctttact
     6001 accaagtaac tgcgactgac aaaggcagtt acaagcaagt tatctatgta cattacatgg
     6061 aaatgcttca ctttaaacaa ccgaggtttg gcggcttgga aggtacagac cccacaaaag
     6121 tattaacgaa taccctcgga tatgatcgag aagtccgaaa aatctcttta agtcagctta
     6181 aaggaagtaa tgaagggcta aaagttaagt ttgctagcaa tatggatgaa gaagctaaaa
     6241 aagctacagt taaaaacatt gctgattttt atcgacaaaa cggtggacta cttgtggaag
     6301 aaaacggtgt agaaatcgaa cgtttacaac gagagctggt agacagcaag cttttagata
     6361 ctgataaagt atctcgctcc agaatcgcta tggtctacaa tgtgccggag catttcatcg
     6421 ggaataacca gtcgagttac tcctctcagg aacagctcaa tatggaattt ttgacgtaca
     6481 atctagtacc gaccgttaat caatatgaag cggaactaaa taagaaaaca ctatcgagaa
     6541 ccgaaaaagc aaaaggttat cgatataagt ttaatgtcgc aagtttgcta agagctgaca
     6601 ctcaggcaag gggacaattc tatcaaatca tgcgacgagg cggagcatac tcagcgaatg
     6661 atgtccgaag atttgaggac ttgcaaccga taaataaaac cggtatggat gattaccata
     6721 tttccggaga cctatatcca atcgatatgg atccaacatt aagaaaaaca acctcgtcta
     6781 aaagcgtagc cgaaaacggt taggtttttt tagtttgcac cgaagggagg tggaaaaatg
     6841 aaaaaagtga cgttaagcgg cgatgtcgtg gataacgata ccgcgtggct ttatgactgg
     6901 tttgggatca attgtatctc accagggaaa atttctgccg ctcttacaga agcagcgggg
     6961 gatgaagtag aacttgatat ctcatcgaac ggtggggatg tcctagcggc aagcgaaata
     7021 tataccgcta tccgtgccta tccgggaaag gtatccggaa atgttgtgag tattgcagca
     7081 agcgctgcaa gtgtaatcgc ttgcgcttgc gaaccgctta gaatctcacc tacggcacac
     7141 atcatgattc ataacgcatg ggtgaccact agtggcaacg ctgaggaatt aaaagccaat
     7201 gcagaaatgt taagcagtgt ggatgagtct attgttaatg cttacgagat caaaacagga
     7261 ctcgatcgga aaaaacttgc tgatttaatg gcgaaagata cttggttaaa tgctcaaaca
     7321 gcagtagcag aagggtttgc agatgaaatt atgtttgcag aagcaccagt aacggtactc
     7381 aatgcctctc aaccggttat tccaaaaaac gcagtaacta agttgaaaaa tttaatactc
     7441 aaagcggaag caccgcaaga agaaacactc ttacagaaaa aactaaaagc cttaaatgga
     7501 gggaaaaacg aatgaattta gaacaattaa aaaatgcgtg ggtcgaggcg ggaagtaaag
     7561 tctctgactt aaatgcacaa ctcaatgcag cattggttga cgatgaaaaa acagaagaag
     7621 atgtagtaag tttgcaagca caagtaaaag cagcacgggc taaacgggac ggattgaaag
     7681 agcaagtggc aaatatggaa gccgaacaag tcttaaacgt caaaaaagaa ccattagata
     7741 aaaaagatga aaacttgaaa aacaagttta tcaaagactt taaagcgatg gtcaatggtg
     7801 atcctgctat tatggctact ttgacatcta atacggatga atctggtaat gctatcggat
     7861 tgactattcc tgtagatgtg caaacgacta ttcatacttt ggttcgtcgg tttgactctt
     7921 tgcaagaata cgtaaacgtt gaaaaagtga ccactaccag cggttctcgc gtttatgaaa
     7981 aatggtctga tattacaccg ttgactgctt tggatactga agacggtgaa atcccagcaa
     8041 atgatgatcc tgcactttac ttgatcaaat acttgatcaa acgctatgca ggtatttcca
     8101 cagtaactaa cagcttgcta aaagatactg ccgaaaacat tttggcatgg ttgtctaaat
     8161 ggatcgcgaa aaaagtagtt gttactcgca atacaaaaat cttggcagct attgatggaa
     8221 tcaaagcggc acaaaagaaa gatgttacag atgttgatgg aattaaagat atcgtaaacg
     8281 tccaacttga tccagctatc gaagctacat ctatgtttat tacaaaccaa gatggcttca
     8341 atgttttaga taaagtgaaa cgtgctgatg gatcttactt gttacaaaaa gacgtagctt
     8401 ctgcaactgg atataccttc ttaggtaaac caatcaagaa aattgcttct cgtttcttgc
     8461 caaacaaagg tacgcaagct actcctaaat atccactgta cattggtgat ctgaaagaag
     8521 ccgttacatt gtatgatcgc gaaaacatga gcttgctgac aacgaatatt ggtggtggag
     8581 cttttgaaac agacaccact aaagtacgcg tcatcgatcg cttcgatgtg caactagttg
     8641 atgatgaagc ggttgttttg gctactttta caactattgc gaacgagaca ccggcgaaag
     8701 tttaaggagc tgattcctta tgattcttga tcctaaaatg gatttaggcg aaatcaaaaa
     8761 cgcactaaag attgataccg atgatgacga tgtggaagta agccgtgcgg cacaagctgc
     8821 aattgcatac attagagggg ctatcgggaa tgataagccc tctttttata agcaagaaaa
     8881 tgaaacggtt gatctgatta atttagctat cctgcaatta gcagatcatt attacaacgc
     8941 tcgatcagca accgtgagtg ggaacttgcg agagtacgat ttaggtttta caagcctaat
     9001 cttgcaactc aaagcaagtt atttgctttt tgtggaggag gagtagcgta tgccccttat
     9061 ccaaacagga aatttaaatc aacgcatcaa gtttgtccga gatacgacgg ttaaggatga
     9121 ggacgggcaa gttgtcccga cttctacaac cattcttact tgctgggcaa gtgtgcagac
     9181 acaacgcctg aacgatatta agacgtcgat tggtacggct ttggaaggaa cactgacgtt
     9241 cattatccgc taccaacaaa aatcagagct gaccaatgat atgaaagtgc gttggaatgg
     9301 aaaaacgttt gaaatcatta cgattacgaa aggcgagttt gcgaaggact tcacgacaat
     9361 cattgcaaaa gaggtttcaa aatgagtgta gaagtcgatg caaccgaagt gtacaaagcg
     9421 cttagggaag taaaagcaaa cgttcaacga gtggaaagcc cagcacttag aaaagctggg
     9481 gagtacgctc aagaagagtt acgacaaaac acaccttact gggatggaac gaagtcaaac
     9541 ggtaaacgtg gttcgtatat gcaagaacat gctaagaacc atgtggttac aagctcgata
     9601 aaaaacggat tggtagaagt cggctatgac aaagatgttt cttggcggat gcactttatc
     9661 gagttcggaa caatcaaaca acgtccaaaa ggtttcgtac aaaaaacaca aaagcaaatc
     9721 gaaaaacaag taacacaaat cattgctgac gaagtaaaaa ggaggctagg actttgaaaa
     9781 cggcagtatc acaagtctat tcaattctga atagcaatga aaaaacaaag aacattgatt
     9841 tttacaccaa tagtgttccg gaatcagctc aaacagtacc tagccttcca gttggcagaa
     9901 ttacagagat atccggcaac tatgaagatt ttgcaagcaa caatcctttg accattcaat
     9961 ttaacgtaca ggtagatgta tgggtgtcaa ccatgaaaga ggttgatgcc ttttattttg
    10021 cccttgatga ggttatgagg gggaatggtt ggcaatgcgc atacacggaa caaacagatg
    10081 acgaggactt ggaaggtgca aagcggatta tcaaacgata tgtagcaaat atttcactaa
    10141 attaaaagga gagaaaatag atggcaacag taggattcga gagcgtcatt tttggcgtaa
    10201 aaacaggtgc aggcggcact ctaaaagaat tagtagcaga taagtcgaaa ggcggagcga
    10261 tcgaagctaa aattactgga ttaggcgcaa cttctaacac aacatacgct tcaaacgtac
    10321 cgttcttcat tgcaagtaaa ggggtttcgt cgccaaaagt tacgcttgac gtggcagact
    10381 taatggataa cggcatttac agcgaaatca ttggtgctaa aaccgtggat ggtgcaaatg
    10441 taattggttc agaaactgaa gcgccttacg tgtcggtagt catggttaca gcgaacaaag
    10501 aaggaaaacg cttgttcatg ggattgacaa aaggaaaatt cagtcatcca gatatcgaca
    10561 tgaaaacagc tgaagacaaa ggggtagaat tgcaaaccga ttccatcgaa ggggaattca
    10621 tttctgatga acgtggctat gtatacttaa cagccgtaga atcagaagga atgaccttac
    10681 aaaaattcaa ggacttggta aataacaaag agggggagta gttaaccctg catctacacc
    10741 aacgacagat acagggacac caaaagaacc agaaccaaaa attgatacac aaggttagcc
    10801 atttttggct agccttattt tttgtaaaaa caaggaggaa aacaaatgat tgaattgcaa
    10861 ttgaaacttg acggaaagaa aaaaacattc aaacaacaag atatttccgc acgtgcaatg
    10921 cgtgagtgta tcaaatttta cgagaaagcg gaaaaagcag acctaactga tttagaagcg
    10981 attgattcaa tgattgcaat tacagcagat attttccaag atccagcagt tacatttgat
    11041 gctattttag acggtttgac tgcgagcgag ttagtaccgg cattagaaag tgtttttgaa
    11101 caaataaatg aactgggaaa caatgaaaaa aagcagacgg cgagcaaaaa gaaataagtt
    11161 tttctgaagc taggaaagca atggatcaaa tctacaaaga tttaatcgaa gcaggttgga
    11221 cgatgagaga tgtggacgaa gccgactatc attatttgtt acaccttttt ggagaagtgg
    11281 agagtggcga agaatatgta gatggtgctg atttcatcaa acaattttta tcggctgaag
    11341 acttagtaaa acttgaggaa ggaggtaaat aatggcagga aaaggacaac cggcaggaaa
    11401 tatcaagcta gggattagtt tagatagcac tagttttggt aacacgctgg acgaaatcaa
    11461 tgcgaaagtc aaacaagctg agtcgaatat gcgtgccaat ctaaaggctt atgattcagc
    11521 aggacgttca tacgaagcac ttagtcaaaa gacgaaagac ttgtctacgg ttatggaagg
    11581 gcaaaacgcc aaagtaagag aattaacaaa gcgccgtgat gaagcgatta gcaagtatgg
    11641 cgaggaatcg aaacaagttg ctaaccttaa cacacagata aacaatgcta ccgcaaaata
    11701 taatgcttac agtcgccagt tgaacgacac aaaaaaagaa ttggtgtatt ccaaaacagc
    11761 cgtcaatgat ttatctaatg aaatcaaaga aaatgaacga caaatgaacg ccgaagtaaa
    11821 agcgttgaaa gccgctggtg atgaatctgg tgcttttgaa gcaaaacaaa aagggctagc
    11881 caaacaaacg gaattatccg agaaagctat cgaagaacag cgcaaagttg tgaaactgat
    11941 ggctgatgag tttggcgatt cagcaaatga aaccgaagat gcaaaaaggg cattagaaaa
    12001 gttagaacga caaagccaaa tatctagcag gcaattagaa gcactcaaaa gctccagcga
    12061 tcaatcagga aaagaaatag aagattttgg cgacaagtcc acaaggtcag ctaggaaact
    12121 ggacggacta aaagacaaat taagctcgct aaaaagcgca ttttcgtttg gtgcagttgc
    12181 tggattagcg cataacgcta ttagcagtgt agtaagtggc gtgcaaggtt tggttggcga
    12241 agcagtaaac gcatcggatt cattgatgaa gttttccaaa accatggagt ttgctaactt
    12301 tgggaagtca cagatagaaa gctcgaaaaa agaaatgaaa gactacgccg ataagacggt
    12361 ttatggttta gaagaaattc tgaacacaac cgcacaattg gcatctaatg ggattcctaa
    12421 ctatacagaa ctaaccaagg cggcaggtaa cttgaatgcc gttgcaggcg gttctagtga
    12481 tacattcaaa tccgttgcca tgatgctaac gcagacggca ggagctggga aactaacaac
    12541 tgaaaactgg aatcaattag cagatgcgat accgggtgct tcaggattgt tacaagacgc
    12601 tatgttgaaa aacggagctt atacaggaaa cttccgtgat gcaatggcgc aaggtcaaat
    12661 cacttccgac gagttcaacc aagcaattgt acagttaggt atgaatgacg gagcagttaa
    12721 ggcagccact tctacagaca cattgagcgg ttcttgggag cagatgaaat ccactgtaat
    12781 aaatgggcta caaagtatta tagaaaaaat aggcgttgaa aatatcactg gtttcatcaa
    12841 cagagtaaca aaagggattg aaaattctat tcctaaaatt actcaattta taggttggtt
    12901 gagagatatt ggaacgtgga tcgttgaaaa tagagagcca ctaacatgga ttgtcggaat
    12961 cataggcgga attacattag cagtaaaagc attgaacgta gcaagtatgt tgctggcaat
    13021 tactggcgga acattggcag ccccttttgt ggcgattggt gtagcattag gcgcactagc
    13081 aggtgctttg gtgatagctt atacaaaatc tgaaacattt agaaatatag tcaatgcggc
    13141 ttttacagct gtgaaaaacg tagttatgag cgttatcaat aatttggtgg aatactacaa
    13201 aatgttgtgg agcgtgttgc agtggctttg ggaaaaaata aaagaatggg cttcatggat
    13261 tggcaataaa ttcattgaaa tgaagaacag cgttgtgaat acggttcaga acctatggaa
    13321 tggcgtgaaa aacttcttca gcaatggcgt tggagatacg tggaataagg tagtcggttg
    13381 ggtaaaaaac attttcaaca aagcaactga attgaagaac aacgtttctg atgtaatcgg
    13441 taacctgtgg aatggtatca aagacacatt ccgtagaggt atcgatacgg tattcaattg
    13501 gttttcagaa ctaccaacga agatgaagaa tgccattatt ggcggtaaaa acgccattgt
    13561 tgatgcgttc aaaagtattt tcaacgcagc acttaaagcg ataggtaaac cagttaacgc
    13621 aatcatccat ggagcttcat gggtactaga aaaattgggc gcagaacctc tacaagaatg
    13681 ggatgtacca caatacgcta caggtacacc agcaggtggg cacccaatca atggtccaat
    13741 gatggttaat gatggacgtg gagcagaaac agttatcaca ccagatggta gagccttcat
    13801 tcctaaagga cgcaatgttg ttctaaacgc accaaaagga acgcatgtct tgacagcaga
    13861 agagaccgcg caacttcaag gttctaaagc tcctaagtat cgttacaaaa aaggtactaa
    13921 cttctttggt aacatgtggg atagtgtgaa aaaggttgct ggtaatgtag gtcacacaat
    13981 taaaaacgta gtaggtgacg tgtgggactt tatttcagac ccgggagcgt tagctagaaa
    14041 agtacttggg gggttagatg tattaggtgg attgacaaag tatccattag aagtaggtaa
    14101 aggcatccta tctaaagcaa caagtgcact gactgaaaaa atcactgggt tgttctcatc
    14161 tggtaactta gatacctccg taggaacaaa tggcgtctat aaatatttgg ctgatgttgc
    14221 taagtctgtg atgaagaaat tcccaggatt tgtggcaact agtgggtata gaccaggtga
    14281 cccttattca catggtaaac gtaatgccat tgatattgca ttaccaggcg tcacaggagg
    14341 ctcacctaga tacacagaag cagcaaacta cgcttttgac aaattcgctt ccaaaattgg
    14401 ttacgtaatc actaatggga aagttcgtga ccgttcagga caatcaggtc aaccagcaac
    14461 tggtgcatgg gagccatggc ccgatggaga tcactatgat catgtgcatt taaacggtgt
    14521 gaaagaccca caaaacactc aaatttcagg agatagcgtg ggaggcagtg gggtagaaag
    14581 atggcgcaat gtagcaatta gagcgttgaa aatgaccggt caatacagta ctgcaaactt
    14641 aaatgcatta ctaaatcaaa tgcgtacaga gtcaaatggt aatcctaatg cagttaacaa
    14701 ttgggatatt aacgccaaaa atggaacacc atcaaaaggg ttgctccaag tgattgaccc
    14761 aacattcaga cagtatgcaa tgccaggatt caacagcaat atttttgacc cactatctaa
    14821 catcttagct tcaatcagat acgcactatc aagatatggc tcactaacaa atgcctatcg
    14881 tggagttggt tacgcaaacg gtggaattgt aaaccaacat caaattgcgg aaatcgcaga
    14941 aggaaacaag ccagaaatta ttattccgtt agataaggct aaacgatcaa gagcgatgca
    15001 attattgtct atcgcaatgg acaaactagg agtttctcct aaaacttatg gaaatgcgac
    15061 aactgtttcc agtgattacg aaatcctaga agccattgaa aaacaggcgg caacaacgaa
    15121 tcagctgtta tcgttattgc tggcattttt caaaggaaat agccgaactg atagagattt
    15181 agctttagat attcagaaga tcttggttag gaggatgtag atgcgaactg tattactgag
    15241 aaatagaata aacgaagaaa ttgatttgtc cacagaagac tattttgcta ctgggttgag
    15301 taatatgggg tttgaagtaa aaaaagaaca tgtgggacag tgggggaatt tcagagaaag
    15361 cagtgagagc gttgaaatat ctgaatttca gtcatctgta atcatttctg tgcatgggtt
    15421 tcgagaaaaa gaactgtaca attcacttgt gcagttccta tcggaaggtc cgtttgaact
    15481 ggaatttgct tttgacggcg aaacaatggt aagaagatgc agtctaaaat ctttaagtaa
    15541 gactgaaatc gatccgaaaa catcgttact aacagacact ttagaactat atttcacatc
    15601 aaactggtat tccgtaaaac gcgaaaaatt aattcagcga ccgaatgtag tgaaaacacg
    15661 tggtaaggtt tttccgtata aaagatctta tatctacact caaaacttgt gggagaaaaa
    15721 aggtgtattc aaattcaata ataattctgt atacctaacg aatagcaagg aacggatgtc
    15781 cccgctaaaa attcgtgtga ttgggaagtg ttcaaatccg tattgggaag taatccaaaa
    15841 ctcacaaatc atcgcaacgg atggatactt catagatatg actgaaacac agactctaga
    15901 agtatcaagc ctttttgaag atacaacggc gattttaaaa gatattgcag gggtagaatc
    15961 ttctgtctac caacaacagg attatacaaa aactaatttt gttcaggccc caactggaga
    16021 attcagcatt gtgtttcatg ttgggggggc agacgtagag attgagttat acgaggagcg
    16081 tgatctgttt tgattttagc agtcacgctt tttcatcgtg atttaatgct ttataacgaa
    16141 catttattct ctaacagttt tgaatttggt gtggacgaga tcaacgaaga ggctagtagc
    16201 tttacgatgg ataagtacgt tcctgtaaaa acaggcgatt ttctactagc aaaatatatt
    16261 cctagtggaa aatttgcata ttttggtgta attacgtcgc aggaagacga aaaaatcagt
    16321 tgtaaaagct tacttagttt agctgatagt gagataccga ctgcacgtgt gtcaggagat
    16381 aactacgaag agcatatccg gcggttgatt gaatactatt tgctgaatga tccaacgaaa
    16441 caactaaaag atattttaga cgtcaaagca gaaagtgcga cctctcattc gtatcaagct
    16501 accgatacga ataaacacaa gttaagtgcg tatattctca atggtttcaa aaaatacaac
    16561 gtaaaatggt atttcaaggg aattcaaaac agaaaaattt atacaggcat acgtgctgta
    16621 aatgaatcaa tttacattaa agacaattct tctgaattta gcgattggga tgtgtttgtt
    16681 caggcgccgg gcgctggaaa cgagaacaag ctattaatcg ttgataaagc aatgaaagat
    16741 atagagaaac cgataatact gtcaacatgg tatttggacg aagaaaacaa tttgacacaa
    16801 gatggatcga aagaaaatat cacgaaacca actgtcaatt tggttaatat ctacgatcaa
    16861 accgcagaag ataaagcatc ctacgaagat gtggcaaaat cagagttgaa aggcaatacg
    16921 tattcgcatg aaatcaaagt gaacgttgta agaaatgcaa aaaatttgaa tgtcgaaacg
    16981 attgaaacag ggatgtttgc cacgatttct tataaaggaa agatatacaa gtcggttttg
    17041 acagcttggc gaatatcaag tgataaggag tttgtggaat tgactttcgg aaacataaga
    17101 agtcgtttta tggattattt tgaagataat gggggataaa aaatggttag caatgtggat
    17161 ggatatcaat ttgaaaacgt gaaagtaagc gcagaaaatg atgctagact ttatcacgtt
    17221 ttatataatc ggaaaaatca ggttattgat ggttacgatc agtctatgaa tttatcttca
    17281 agcggattaa cagtaaaagt tgctgcagga gcagcgatta ttcaaggtcg tatggtcgtt
    17341 gttcgacaag aagaaagtat aacagttcca gcaaactcaa gtggttatat agcattaaca
    17401 gtggatttga cacaagaagt tatacctgga tctattcttc cagaatcgga agaatatgaa
    17461 tggactaata atcaagtcaa gctagaattt ataacaaaag ttataaaagg taatttaaac
    17521 aatggtgata aggtctataa tttaccacta tgctcagtta attctactgg atcaactgtt
    17581 tcaatctcaa agatatcgga tagttacgag ctgactctct ctaaaggaga aattttgtgg
    17641 agggggactg cgttaatgca tgatactcaa actgtccaac cttcaaaaaa aatttggcag
    17701 acagtttcag gttttttgtt agtatggctc ccatacgaaa acgggcaagc aattgaggat
    17761 agatatgtaa ctacgccttt ttataaggag cgtgtaactt ttactaatgt tttaggagaa
    17821 attgtttcag gatttgatga ctatcacaaa aagtggttta gtaaacgaat aaactataat
    17881 tcgaatacta atatatttac aggtgcagca agcaatgcaa gtggagataa cgcaaatatg
    17941 gtgcttaggt atatagtttc tttttagtta ggaggtatgg aagagtggca aatttagaaa
    18001 ttaaattatc tgcaaataag agacagcctt atctacgtca ccgtgttgtt ggtagagttg
    18061 gtgatggggg gctcacaaca atcaatgtac aacttcttga ggaagatgaa attacacctt
    18121 ttgtaattaa tctaaacggt actttgaaat ttgtgggcga agtttcaaac ggtaactata
    18181 ccgagggaga accagaaata atcgattcga ctaatgggtt aattagttac acgttcacta
    18241 agtcaaattt cagcacgagt catcaattca aacaagcata ttttgaatat gtagatccta
    18301 acggcaaaaa agtaactttt cagaacttca tcatagacgt gttagaacga gtggatatta
    18361 attcggagca agcgaaatac tatatttctt cattggaaaa attacagagt gaaatgcaaa
    18421 ccactttcaa tcagttcatt agtgataaac aggtccaata cgatcaaatc tactcgaaat
    18481 ataacgaatt agtaagattg ataaatgaat cagataagca agtaaatgat cgtattgatc
    18541 aaaccaatca gcaaatcggc gatctcggca agctgaaaaa gatgtacagt aacagcatcg
    18601 actttggggg ctatgattat tcggggaatc cgaatttgtt aagtaagcta tcatacgact
    18661 taattgaaaa tcaaaatact tcagctggaa cactttctaa gggtgaaaac tcgtttaaat
    18721 ataataagat atcagctgaa gtggaaggtg gagtagagtt atattataaa cgaagaggta
    18781 tagctaactg gttaccctct aataaaacgc ttgtaatgac tgttaagctt agagctggag
    18841 cggactatag tccagttgac ggaaaactta tactgattag atataggtat gttgacagtg
    18901 gaactggcaa gattgtttta gacttaccta ttaacagtaa ttcgataact caggaatgga
    18961 aagagtttag tattactgga actactccaa catttagccc acaagcatac catccttgga
    19021 tacaatttag ggctcaagat ggggtacttg gggaaataga aatgagctat gacatcaaaa
    19081 tcgaagaagg ctctacagct acaccatatc agcctaactt acttgatgac tcttactggc
    19141 taggtaaaac accgttgggt gagaatttga taacgaatgc caagttccca attataacac
    19201 gaagcaatcc tatttctggt tttgatattt cagaagaatt aataataggt gaaacttata
    19261 cggtatcttt aaaagggact aagccagcta ctaaagaatt ccacttatac tacggagaag
    19321 caaattacca gcagttgcag tatcaggcaa ctttacttcc tgtggaagga ttaacagatg
    19381 tgtggagtgc cacttttaca gccagaaata ctagtgaaac gacaaattta cttagagtag
    19441 cgttatggca aaaacctaat tctgcaacat atgatactgt tcaaattgac tggctcaaga
    19501 ttgaaaaagg cgacacacgc acaccaaata tcagtcagtt taaatacttt ggtgaaggct
    19561 tgaaagacag taacaatccg aacgactaca gctgggatgt cacaccagag tatactgaaa
    19621 aaagcttgaa taatacggtt agtctgactg aaccgcaatc catcgaagga ttgaaaaatt
    19681 tcgaagatgg tattcaatca aaaggaaaat ctgtattgac atcagacgac aacaaatatg
    19741 aagtcgtaac cttaacagtt acaaacggga ataccggatc agcaaagctt tatcgtgaag
    19801 gaaaaaccgt cactatttat ttttttgcgt tgaatgggaa aagcagtggt ggaaatgatt
    19861 caacgatact aacaattcca gaaggctatc ggccaccaat tagttttgag caactggttg
    19921 gctcgataga ccgttctact ttgaacagtg ctcagttatc tattggtgca gatggagcca
    19981 ttaaatggcg aagaaactca agttatggat cggattatac ctttgcaatt acttacacga
    20041 tttagaaagg cgtgaatcga tatgaaggca gcatatagac caattgaacc ttacggattc
    20101 gagcaaatca ttgtgaatga tgaagaacat ttaccggaag aatgcacaga agtcgaacca
    20161 ccgattccaa attggaaacc gaaattcaat tactgggagg gaaataaatg aaaaacattt
    20221 ggaaatatgg ccgtactggc ggagagtatg caggaaaagt attggacgac atgcttgtat
    20281 ccgttcctta cacagatcag ccaccgctcg aaggggttcg ttcagatggc gaaccgctaa
    20341 cgattgctga tcagatgttt gatcctaaat tgaaccaatg gattatttta gcgaacgcgt
    20401 tagatcacaa cgatttaaac aatctcaaag cgatgtacga ggctcttgaa catgaaaacg
    20461 gcaacctaaa acagctaaat gccaaactca tgctaagcga tgtagcgatt aaacaggaaa
    20521 atactgcatt gaaagaaaaa gctgacagtt tagcacaaat caattcaaaa atgatgcttg
    20581 cttcgttaca aaatagcaaa gacatttcag aaattaaaga gcaactaaat ccagcttcaa
    20641 agggaggtga gtagtatgtt tagttttagc gatgtgaaaa tgatgtatga ttggggctgt
    20701 tttactgacg atcaagttcg tctattcgtt ccactatgta tcacagatga agaagcagat
    20761 agaattatta gcaaagaaga gagcgcatct taagtgatgc gtttttttgt tggaaagttg
    20821 gtggaacatg aaagaagaag cgctccaaga cgttgtggag agattagtaa gaattgaaac
    20881 aaaattagac aactacgaat cacttagaga aaaagctgat agtgcaaaag atttggcaga
    20941 caaagcctac tcagtagcgc taaacaacgc agaagatatc aaagaaatga aagccaataa
    21001 taaatggtcg tggggttaca tgatcggttt aggcattacg atcattggct atttcttgac
    21061 taaattgtaa aggagttaag aagaaatgat tttacccgat aagtattatc aagtcattaa
    21121 atggacggtt ttaacagtat taccagctgc atctgtttta gtagccacgt taggaaaagc
    21181 gtatggatgg aatggaacag atatgacagt actcactatc aatgcagtag caacattttt
    21241 aggcgttatc actggtgtgt ctgcatataa cttaaaaaaa taggaggaaa aaaatgaaga
    21301 agaaaatcat tttatcattg agcctaataa tggctctttt tttattgcca attaacgggt
    21361 tcgcctatac gatcaacaat gaatttaatt taggtgtaaa tgaaggtagc tcacaagtag
    21421 caaataacca atacatccta ttgcatgaaa cagcaaacga aactgcgaca ggacgaaatg
    21481 aagcgcagta catgaaacgt tcttggtaca atgcctatac agcgtatatt gttggcgatg
    21541 gtgggatcgt ttaccaagtt ggacaacctg gttatgtaca atatggcgct ggttcatatg
    21601 ctaatgcaaa tagtcctgtg caaatcgaac tgcaacatac gcacgataaa gtaacgtttg
    21661 aaaagaacta caaggcatat gttgaattag cgagagattc cgctaataaa tacggtattc
    21721 cacttacatt agacacaccg tataatcaac caggaatcaa atcacattta tgggtaactc
    21781 aaaacatttg gggcgatcac actgatccgt atggatactt atcagaaatg ggtgtaagta
    21841 aggaaaaact ggcttacgat ttagctcatg gatttacaga cgaaaaccca acgacttctg
    21901 agaacaagcc tgtcattgat ccaacccgag caggtgcagc aaatccaact ttatcagatg
    21961 gaacgaacca ttctcacatt gatcagtttg gagaaatcga aaatgcgaac ttacacgtcg
    22021 ctggatggca catcgctaac tataaatacg agtatatttt cattatggac tacaatactg
    22081 ggaaagaatt agctcgagta agagctgatg gaatttatag accggatgta aatcaagctt
    22141 ataatacttc tggaaacgtt ggctatcatg tatcttttaa catgcgcaat ttccctaata
    22201 agaaagtata cgtcatgatg cgggcaacga atgatccaga aggaaacact aaaggcggtg
    22261 cgcaagattt ccatgacaaa cgttggtatt taaatattcc taaacgataa aaatagctcc
    22321 tcgttgagga gcagtacata gaattgaaaa ttaacgttaa atcaaaaaaa tatttacaag
    22381 atgtttattt aagtagataa taaaagaaaa aagcaggtga aattgtgaca gcagaaattg
    22441 gtattatgaa taaaagtgga atcgtgttag cttcagattc agcatctacg attggagata
    22501 gcaaagtata taatactgca aaaaaattat ttactttgga ttccatgcat tctgtaggta
    22561 tcatgatata tggtaatgct gaatttaatg gtattccttg ggaaataatc attactcaat
    22621 ataagaaaag cattggtagt tctgttttta atactttgga agaatacgcg gataatttta
    22681 tagaatttgt caaaacagca tcttttatta gaagtgaaca gaccgagcaa gaacaaatga
    22741 ttggggtttt tcaaaaaata atttcaggtt tatttgagag tatagaagta gatattaact
    22801 ttttaataag ccaaggtaca caaatagaca aagatgtcct agtgaagtta ttacaaacaa
    22861 aaatgaatac caatttgtct caacagtccc agacatt
//
LOCUS       DAZKLA010000034        22765 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000034 DAZKLA010000000
VERSION     DAZKLA010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.034"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACG85N_001138"
     CDS             complement(132..509)
                     /locus_tag="ACG85N_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223922.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /gene="purC"
                     /locus_tag="ACG85N_001139"
     CDS             765..1493
                     /gene="purC"
                     /locus_tag="ACG85N_001139"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFQ0223923.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="ACG85N_001140"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="ACG85N_001140"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFQ0223924.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="ACG85N_001141"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="ACG85N_001141"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFQ0223925.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="ACG85N_001142"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="ACG85N_001142"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFQ0223926.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /gene="purF"
                     /locus_tag="ACG85N_001143"
     CDS             4628..6067
                     /gene="purF"
                     /locus_tag="ACG85N_001143"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFQ0223927.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /gene="purM"
                     /locus_tag="ACG85N_001144"
     CDS             6090..7142
                     /gene="purM"
                     /locus_tag="ACG85N_001144"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFQ0223928.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="ACG85N_001145"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="ACG85N_001145"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFQ0223929.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="ACG85N_001146"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="ACG85N_001146"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFQ0223930.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="ACG85N_001147"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="ACG85N_001147"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFQ0223931.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="ACG85N_001148"
     CDS             10661..11575
                     /locus_tag="ACG85N_001148"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFQ0223932.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="ACG85N_001149"
     CDS             complement(11708..13273)
                     /locus_tag="ACG85N_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0223933.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="ACG85N_001150"
     CDS             complement(13266..14717)
                     /locus_tag="ACG85N_001150"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0223934.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="ACG85N_001151"
     CDS             complement(14731..15747)
                     /locus_tag="ACG85N_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0223935.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /gene="chvE"
                     /locus_tag="ACG85N_001152"
     CDS             15967..17055
                     /gene="chvE"
                     /locus_tag="ACG85N_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0223936.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /gene="mmsA"
                     /locus_tag="ACG85N_001153"
     CDS             17109..18635
                     /gene="mmsA"
                     /locus_tag="ACG85N_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0223937.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /gene="mmsB"
                     /locus_tag="ACG85N_001154"
     CDS             18637..19842
                     /gene="mmsB"
                     /locus_tag="ACG85N_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFQ0223938.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="ACG85N_001155"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="ACG85N_001155"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFQ0223939.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="ACG85N_001156"
     CDS             21340..22764
                     /locus_tag="ACG85N_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0223940.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
BASE COUNT         7332 a         3840 c         5156 g         6437 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataat
//
LOCUS       DAZKLA010000035        22719 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000035 DAZKLA010000000
VERSION     DAZKLA010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.035"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..1942)
                     /locus_tag="ACG85N_001157"
     CDS             complement(11..1942)
                     /locus_tag="ACG85N_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFQ0223941.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="ACG85N_001158"
     CDS             complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="ACG85N_001158"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFQ0223942.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2871..3623)
                     /locus_tag="ACG85N_001159"
     CDS             complement(2871..3623)
                     /locus_tag="ACG85N_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0223943.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3782..3991)
                     /locus_tag="ACG85N_001160"
     CDS             complement(3782..3991)
                     /locus_tag="ACG85N_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223944.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4002..5330)
                     /locus_tag="ACG85N_001161"
     CDS             complement(4002..5330)
                     /locus_tag="ACG85N_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223945.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            5466..5621
                     /locus_tag="ACG85N_001162"
     CDS             5466..5621
                     /locus_tag="ACG85N_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFQ0223946.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5695..6282)
                     /gene="yihA"
                     /locus_tag="ACG85N_001163"
     CDS             complement(5695..6282)
                     /gene="yihA"
                     /locus_tag="ACG85N_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFQ0223947.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="ACG85N_001164"
     CDS             complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="ACG85N_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFQ0223948.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7862..9151)
                     /gene="tig"
                     /locus_tag="ACG85N_001165"
     CDS             complement(7862..9151)
                     /gene="tig"
                     /locus_tag="ACG85N_001165"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFQ0223949.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9305..10237)
                     /locus_tag="ACG85N_001166"
     CDS             complement(9305..10237)
                     /locus_tag="ACG85N_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223950.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10394..11053)
                     /locus_tag="ACG85N_001167"
     CDS             complement(10394..11053)
                     /locus_tag="ACG85N_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0223951.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="ACG85N_001168"
     CDS             complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="ACG85N_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0223952.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12094..12384)
                     /locus_tag="ACG85N_001169"
     CDS             complement(12094..12384)
                     /locus_tag="ACG85N_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223953.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12793..14598
                     /gene="glmS"
                     /locus_tag="ACG85N_001170"
     CDS             12793..14598
                     /gene="glmS"
                     /locus_tag="ACG85N_001170"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFQ0223954.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14670..16025)
                     /gene="glmM"
                     /locus_tag="ACG85N_001171"
     CDS             complement(14670..16025)
                     /gene="glmM"
                     /locus_tag="ACG85N_001171"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFQ0223955.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16049..17218)
                     /locus_tag="ACG85N_001172"
     CDS             complement(16049..17218)
                     /locus_tag="ACG85N_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFQ0223956.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17215..18102)
                     /gene="cdaA"
                     /locus_tag="ACG85N_001173"
     CDS             complement(17215..18102)
                     /gene="cdaA"
                     /locus_tag="ACG85N_001173"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFQ0223957.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18291..21983)
                     /gene="nifJ"
                     /locus_tag="ACG85N_001174"
     CDS             complement(18291..21983)
                     /gene="nifJ"
                     /locus_tag="ACG85N_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFQ0223958.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22182..22703
                     /locus_tag="ACG85N_001175"
     CDS             22182..22703
                     /locus_tag="ACG85N_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFQ0223959.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         6326 a         4932 c         4041 g         7420 t
ORIGIN      
        1 ttttttattt ctatgcttct gctactgcag gagcttttct caataaacca taaatcacac
       61 cagatacgac tgctccgata gcaataaaca ccaaatacat gaccgggtga ctcagtaaga
      121 atacaacgaa aatcccgccg tgaggagcca gtaatttgat tccgaaagct ccgactaatc
      181 cgccagttaa agcagaacca ataataaaac ttggaatggc acgaatcggg tcagctgctg
      241 caaatggaat cgatccttcc gtgacaaacg atagtcccat cacgatgttc gtcaaaccag
      301 catcttgatc tgttttgctg aatttattct taaacaaacg tgtagcaacg aaaattgcca
      361 atggaggtac cattcctcca gccataacag aagccatcac gacacttccg ccagttgcaa
      421 cagtcgtagc taaagttgct gtaccaaaca cataagcggc tttgttgatt ggaccgccta
      481 aatctgccgc catcattcca gctaataatg caccaagtaa agcagcattc gaaccactca
      541 tacttgtcaa gaaaccgttc aatgcatcat tgattgcttt catagggaca ttgaccagca
      601 acatcaagaa accagtaatc agtaagccaa acactggata aaagaggatc gttttaattc
      661 catccaatga ttttggtaag ttcttgaata atttacgtaa aaatacgatg acataaccag
      721 ctaagaatcc accaatcagt gcacctaaga atcctgcacc gcctgtatta gctaatgcac
      781 cagcagcaaa tccaacgatc aaaccagggc ggtcaccgat acttgaagca ataaatcctg
      841 ctaataccgg cagcatgaaa ccaaatgcag cttgtccgat ttggttgaac cagctagcag
      901 cttgattgta attccctaga tttgataatt gatcatgtgg cacgccaata aattggtcga
      961 tcatgaaaga aagcgcaata gcgattcctc caccaataac gaaaggtaac atgtgtgata
     1021 cgccgttcat caaatctttg taaattcttt gtccgatagt accatccgca tttccttctt
     1081 ctgaactgct ttcttttcca tcaccatgga atactggtgc agtaccacta agggctagat
     1141 tgatcaactc ttctggttta cggataccat cactgacagg acgattgatc agttcttttc
     1201 cgtcaaagcg attcatctct acttttttgt ctgctgcaac gattacacct tctgcacggg
     1261 cgatgtcttc agcagtcaat cggtttttga ttccttctga accgttcgtt tcgactttga
     1321 tctccacacc catttctttc gcttttttct tcaaagcatc ttctgccatg taagtgtgag
     1381 cgatccctgt tggacaagct gtaacggcaa caacgaattt tctttctgga tgagcagaag
     1441 cttctgcagc ttgtgtttct tgttctgctt tttcttctgc ttctttttgc tcttcagctg
     1501 tttggaacaa gtcttggact tcttctggtg tatttgcttc ttttaatttt cctacgaaat
     1561 ctgcatcaat cagcaatcga gatagtgctg ctaatgcttg caaatgtgtg tcgtttgcgc
     1621 cttctggagc tgcgatcatg aaaaacagat aagttggctg tccgtctaag gcttcataat
     1681 ctactccttt ttggcttttt gcgaataaaa ctgttgcttc tttaactgca ctgtttttcg
     1741 cgtgaggcat ggcgattcca tcaccaagac ctgtagaagt ttgtgcttcg cgggctagaa
     1801 ttccttcttt atatgtttcg atatcggaaa tccggccggc atcaaacatt ttttggacca
     1861 tttcatcgat tgcgcctttt ttatcagtcg cttgcaaatc catgatcatc gcttctttga
     1921 ccattaaatc cttgatattc atgattatcc tccttgaaac taatcaattt ttgtgatttc
     1981 aacttctggc agcaactcat caatcaattc gcgagctgcc aagtcatcag aaaaagctgt
     2041 tgcgcttccg caagcaactc cccatttaaa ggcttcgatt ggatctttcg tacggctgta
     2101 agccccaata aagccagcaa tcattgaatc ccctgcacct actgaatttt tcaatgggcg
     2161 aactaataca ttcgttttat aagttccttg ttccgtgaag agcaaagcac cgtctccagc
     2221 cattgagatc agcacattct gtgccccttc agctagtaat tttttcccat acggaatgat
     2281 ttcttctaca gattggaagg ttgtgttgta aagctcagct aattcgtgat tgtttggttt
     2341 gatcaacaac ggtttcttaa caagtgcatt ttttaggtca tcacctgttg tatcaatcac
     2401 gaaatctgca cctttttctc gaatgatatc aatcagttct tgataaaatc cttttcgcag
     2461 gctagctggc gcgcttcctg acaatacgac gatatctcct tgtcctactg ttcctaatgc
     2521 ttgcttcaac tcatcgattt cttcatctga aatagatggg cctaagccat tgatctcagt
     2581 ttcagtgtct gattttagtt tgatattgat tcttgtatct tcttttacgg ttgtgaacaa
     2641 agactggatt tgttcttttt caagccactt cgaaataaat tcgccagtga accctcctaa
     2701 aaaccctagt gctgtagatt gagaatcgat ccgtttcaaa atacgggaaa cattgatccc
     2761 ttttcctcca ggtaacttga agtcttcctt cattcggttc aaatcgccta gttttaaccc
     2821 atctacgcgt acgatataat caattgatgg attaagtgtt actgtgtaaa tcattgtatt
     2881 gcctccttaa ttgttgtctt ttcacggaat tcattaacga attctaatgg acaacgctcg
     2941 gttatgatcg tggctgcttc caaaggtgca acagaggtga aggacaactg gttgaatttg
     3001 gtatgatcaa tcaatacaaa tgtctcttcg gaatttttca tggctaaacg tttaacagct
     3061 gcctcttctg gatccggagt cgtgaatcct tgttcaagat gggcaccgtt cattcccata
     3121 aatgctttat taaaatgaaa attttttagt tgttctatcg cattagatcc taaaacagca
     3181 ttagttgcca gctttatcgt tccacctaaa ataatggtag caagctccaa atcaactaaa
     3241 tttgccgcat gttgaacgga atttgtcact actgtgatct gttttccttg cagaaaagga
     3301 atcatttcta acgtagtaga gccagcgtct aaataaatga catcttcgtc attaatggtt
     3361 tcagctgccg atttggcgat ttgctgtttt tcgtgaacgt ttttgagtga tttttcactc
     3421 atattctgct caaaattcaa taattgctct ttcttagctc cgccatgaac tctttctaga
     3481 aacccactat tctccagctc ttgtaaatca cgtcttatcg ttgattcaga agcattcaaa
     3541 aggtttacaa gatcttgtga tttcacgatt ttctgctgtt caagcaagtc taggattttt
     3601 tgtctgcgtt cctctgtaag cattttcacg atcacctcaa tacaaataat agcatatttt
     3661 ttttctacta gcaatataaa tcagtcaaaa acgttcataa aaattataaa tcagtcaaaa
     3721 aaaaataaaa aaccagtcag aaagactggt tttttatttt tttgtctatc atcaagccgt
     3781 tttatcgttt tttattttcg cttcttgttt ttttcatata aacagaagca ggttttcttt
     3841 ccttatgaag aagataataa taaagtccca tcccgccaaa catgaccaaa aaaccgatga
     3901 ctgatcgaat aggaaaatta ggaacaaaaa agagacagac ggataataat gatgccggaa
     3961 ccattattcg ataatagagt tgtttatcca ttttcttcac ctcatatttt ttgtaaagta
     4021 atcaattgtt ctaattctcg gtaatctaaa aattgcacac gcagatgctg ctcacctttc
     4081 ttgacatcga attggatact tcgccgcttt ttctttattc gtttgaaggt aacatcagag
     4141 agtctatgtt tgatttcctt taacggtaat tctgacaggg attctgaagg aacatagatt
     4201 cttaaggata aaagatactc ttgatatttt tcatctgtta cctctacttc tagatattcc
     4261 ctaatataat aacaataagc ttcgattcca ttatccaaag caacagtcgg atcgataact
     4321 ttatagcaca tcatgcgtga tcgattctcg tcaacccaag gaaaatacgc ctgagcattt
     4381 agtgttccgc gaacacgcct ttctttataa tctccttgtt ggatatcata actttttgtt
     4441 acaaaaaatg aagcattctt tttaggaaca ggttgcattt gtgcattctg atactggaca
     4501 gattggatcg tatggtcagt gtaaatgtat tcaacgaatg gcggtttcaa taaaccgaca
     4561 ccattgtccc aagcaagatg atcaaaccca agctggtccc taaaatagga ttgaaacatt
     4621 tgattgtttg gcttttgcca gttaaagggg tctcgataaa gctggataca tatgacttcg
     4681 ttttctaatt ccttcacttt ttctacttgt tggatgtcga gaaaaatcgg tttagggatg
     4741 atttgatgat aatagcgact ataatacatt tcccaacacg aagtgaaaca taaaccttgg
     4801 taaaaaagat cgtatcctgg aaattggttg caatcgacta ctacttcccc gttttgttgt
     4861 ttcattttag gtaaatgttc gatttcatcc tttttctcaa acaatagccg ttgagagatc
     4921 tgttttgtat tgtgatagat aaactcgcta taagctcgca tataggcgaa caccccatga
     4981 gcaatcattt ttttctgaat gaagtctcga atcaaaaatt gcttttcttt gaaaacagag
     5041 atagacaata cattttttat caataattga ttatcttcca agcgaaaact acattcgttc
     5101 tcgtcatttc ctacaactaa ccaactttcg tctcttaccg atgctagcat tattttttct
     5161 tgaagctttc gaagatcata cggatccgct tgttgtgtga aagagcggat aaatgttggc
     5221 tggatcaaca gacgtgtcaa aatagcccaa tcgcttattt cctgtttttt ttcttcttca
     5281 tttaagtctg tgagctggat agtcatcaat aatttatctt cgccaatcat tttctcacct
     5341 ctttcaaatc tttttataat ttaattataa ccaatgataa agaaaaacta aaactttatg
     5401 tcttagaaac caatttggac gattacatga tagactaagt aaaaagaaac aaagaggtgt
     5461 ttacaatggg gttgaaattt gaacgtacag ataatttaga taaacttttc gaatcatttg
     5521 ctgtcgatcc ggaaaaaaca gttgcaccaa aagaagagat cgatcgattt ctaaaaccac
     5581 agccacaaaa tgaaaagaaa aaagacaaga aacaaaagta agttaagctc aaaaaaagac
     5641 ttaccagcca aacttttatg aaagagtttg actggtaagt cttttttatt ctttttatga
     5701 ttcaattgct tgttcgattg ctgcccacgc ttcttctttt ccctgttttg taacggaaga
     5761 aaagagaata aacgtgtcat tgcgatcaaa atccaacgct tttttgattt cgctctcatg
     5821 cttgttccat ttaccacgag ggatcttatc tgccttagtg gcaacaacaa ttacagggat
     5881 atcataatac ttcaaaaatt gatacatctg gatatcttct tgagaaggct tatgacgcaa
     5941 atctactaaa gaaacgaccg cacgtagctg gtctctagtc gttaaatatg tctcgatcat
     6001 ctttccccat tttgctcgct cagttttgga tacttttgca tacccatatc ccggaacatc
     6061 gacaaaatgt aatgcgttct cgatcaaata aaaattcaat gtctgggtct ttcctggctt
     6121 accggaagta cgggctaaat tctttcgatc aatcaatgtg ttgataaaag aagatttccc
     6181 tacatttgat cgtcctgcca gagctatttc tggcaattgt gtatctggat attgttttgg
     6241 tgccactgca cttatgataa tttcagcttg atggactttc atggtctctc tccttaacaa
     6301 ggtgatgaat aaagcaagcg gctgtcgcaa aagccaaaat gacttttacg gcagcctctt
     6361 ttcatcaacc cgcttttttc tctgaatctt tatagattac tgttggctca ccagacaatt
     6421 cagctgcttc ttttgtgata atcacttttt cgatgttttc atttgaaggg atatcaaaca
     6481 tcacatccat catgatttct tcaatgatgg aacgaagtcc tcgagcaccg gtattgcgtt
     6541 cgattgcttt tttcgcaatg gctctcaacg cttcaggttc gaattcaagt tcggtattat
     6601 ctagagaaag aagtttttga tattgtttga ctagcgcatt tttcggctca gtcaagatcc
     6661 gtaccagatc atctgttgtc aatttctcta aagctgccat aacaggtaat cgtccgataa
     6721 actcagggat caatccgaat tttagtaaat cttcaggaat gatatgctgc atgacacttt
     6781 cgtcttcaga aagtttttgg ttttgtgtac caaatccaat cgtcttttcg cccatacgat
     6841 tcttcacaat cgtttcgatg ccatcaaatg caccacctac gatgaacaat atgttcgtcg
     6901 tatcaatctg aataaattct tgatgtggat gtttacgtcc gccctgaggt ggcacacttg
     6961 caacagttcc ttctagaatt ttcagcaagg cttgttggac accttcgccg gaaacgtcac
     7021 gagtaataga tacattttcg cttttacgcg caattttatc gatctcatca atatagatga
     7081 tccctttttc tgcaagttcc acattgtaat cagcagattg aagcaattta agcaaaatgt
     7141 tttctacatc ttcccccaca taaccagctt ctgtcaaact tgtcgcatct gcgattgcaa
     7201 aaggtacatt caatgttttt gctaatgttt gtgctaaaaa tgttttacca gaccctgtag
     7261 gtccgatcaa gcagatattg cttttttgca gttcaacatc atctgtgttt tcttgtgctt
     7321 gctgattgac acgtttatag tgattgtata cggcaacagc taacgagcgt tttgcgcgat
     7381 cttgtccaat cacgtagtta ttcaatactt caagaatttc tagtggttta gggacttcag
     7441 tgaattcgcg tacagcttct tcataaaatt cttcatcaat gatttcttta cataggtcga
     7501 tacattcatt acagatgtag acccctggac cagctacgat ctttttcact tcttcttggg
     7561 ttttcccgca aaaagaacag cgtacggtct cattgctact tggattgtca tacatggtct
     7621 tcacccctta acttgcaaaa agtatgcaag aaactatcaa agactttaca gtcttttact
     7681 atcataacat agtttagtaa aaagaaaaag tgtttaacaa aggatcaact gacctatacc
     7741 tttgcatttt ttactcttaa caaaaaataa ggaactgtga caaaatcgtg tcacagtccc
     7801 ttatttcgaa taaacaattt tctagaagta gcttcttcag aaatgattta ttacttgatt
     7861 tctattcttt cgcttctgtc gctgtacctg tgatcaattc gattgctttt ttcattgtca
     7921 catcatgttc cagcatatct tctgtcaaca cgcgtttgat ttgttcgatt ggcatgttgt
     7981 atgtttctga taattcttgg atttctgcat tgatttcttc ttctgttgct tgaagatttt
     8041 cagcagctgc aattgcttcg atcacaagat ttgttcttgt gcgcatttct gcttcacctt
     8101 cgaattgttt atgcaagtct tcttctgtag aaccagttaa ttgatagtac atttcaggag
     8161 agataccttg acgttgcatg ttgttcaaga attcgtccat tgaacgatgt acttcatcat
     8221 gaaccatcac gtgtggcaat tcaacgattt ctgcgttttc aacagccata cggatcgctg
     8281 cttcatcttt tgcatcgtcc gctgcttttt cttttgtttc tactaattct ttgcgatatt
     8341 tttctttcaa ttcgtctaat gttgatactt cgtcatcaac atcttttgcg aattcgtcat
     8401 ctaattcagg cacttctttt gcttttactt catggattgt cactttgaag actgcttctt
     8461 ttccagcaag atcttctgct tgataatctt ctgggaaagt tacatttaca tctagacttt
     8521 cacctgattt atgaccaact aattgatctt caaaacctgg aatgaatgag cctgaaccta
     8581 attcaagtga gtaattttcg ccttttccgc catcaaacgc ttcgccatct acaaagcctt
     8641 cgaagtcgat tactactgta tcgccatttt cagctgcagc atcttctttg ataactaatt
     8701 cagcttgtgc ttcttgttca cgtttgatac gtgcatccac gtcagcatct gttacttcac
     8761 ggtcttgttt ttcaacaact agatctttat attgtcctaa ttttacttct ggtttcactg
     8821 ttacttcagc agtgattacc cagtcttgac ctttttccat gctttcaaca tcgatttttg
     8881 gttgagaaac aggatcgatg ccagcttctt tgacagcagc ttcataagct tctggtaata
     8941 cagcattcaa tgcatcttca tataaagctt cttcaccgta catacggttg aatacttgac
     9001 gagaaacttt ccctttacgg aatccaggta cgttcaagct ttttttcact ttattaaacg
     9061 cagttgttaa tcctttttgg atttcgtctt gcgcaatcgt aaattttaaa acaccatcat
     9121 tcgtgccttt tttttcaaat gttgcagtca tttaattccc tccgagttta gaaattcatt
     9181 ttatacatca aaaacgtttc atgcatacct ttaaatagta cactaaccct ttcaaattgt
     9241 aaacatattg aatggataga atccttgttt ttcataacat ttttatcttt ttcactgata
     9301 aaatctaatg gaagaagatc atttgctgca aaattttttc tctttttttt aaaaaatctt
     9361 gtttttcatc ttctgtacct tcatacatcg tttttcctgt acacgcctct acagtcagtt
     9421 gaaaccagcg cttcgggtct tcgatgtatg tatcatagac aggataaaca attgcagatt
     9481 gcaacgtgaa ttcttctttt aacacggaac taagcagcgc atcttcattc ccgtaacgat
     9541 ctgaaaatac acataattgc tggtaacttg cctgctgctg cggctttggc agatcttttg
     9601 gtatgacttt gactaacttt tcttcaatcg tcaaatagaa aacttgatgg cattcgttca
     9661 attgcctaag tgtctccagt aattttgccc ttgctaaaat atggacctct ggcgatacaa
     9721 taagtttact cgctgtttgg ataaactcat gataaggaag ctgttcgatc tcatcaatag
     9781 acgcaagctg acgtgcaggt tccaacgaag gaagttcctc cgatttattg tggagtgcct
     9841 tgatcttctg cctctccatt tgtccggata attcttctgc aaattggatt ttttcaatga
     9901 cccgctgctc gaaagcttca taaaaatctg tacttgctat caattttcta gcatataaaa
     9961 aatcttgtgt actgattaac aagtcaaaat aaaattcagc aaactcctca ttttgaagat
    10021 actgtttttc atgcaagagt gcttgcttca atgcttcttt tttctcgtcc aactcgtaaa
    10081 gacaaaaaac aagcaggcta ttagcctcaa agctttcttt caactcgtaa gctcgttgaa
    10141 ataattcttt tgcctgctga tattgctggt ttgctagtgc ttgttgtgcc actcgcatta
    10201 aattctcata tttttctgga aaatcaatcg gcttcacgca gtgtcctcct cttgctcata
    10261 ttcagctcat gttcaatagt agcaaaacaa aaaaagcatg acaattaaaa ataactgaag
    10321 caccctctaa aagctagatt ttagatctga cttttagaag gtgctttcaa ctgttactgc
    10381 tttttattgc tcatcagctg ttgacttcct ggttgaacgc tttgatcaga ttgatcgtaa
    10441 caacacttgt attagtgaaa aaatcgccca gatgttgctt tttctgatta aagactgctg
    10501 gaagataccc taacatcagc aaaggaaatt ttagaatgaa ccggcaagca ccttctctaa
    10561 ctaaaacggt tgaccagcta agttcttctt catcaaagct gatgactcga ataccaaaga
    10621 tcattttccc aatcgtttgg ccatgattga atttcgtcaa taaaataaaa tacgctagat
    10681 aaataagtaa ggctgtgaat ccgtagagac tcagataaga atctgaaaaa ttaagccaac
    10741 ctagattttt ggcaatacca agtgttaaac gcgtaatagc tccgatacac aaaagatcga
    10801 ccaagaaggc aaacattcgg ataaaaaaac ctgcatagaa atacttagga aaatcatttg
    10861 cttttggcgg ttcatttctt gtgtccgtat actttttcca tttacgttgt ttctttttga
    10921 tttcttcggg cgtcaaaggc tctctttcaa aagagctcag taccttttgg gaaaagttgg
    10981 agtcaggtcc tacagcaggc tgctgttgct tatctttttc ttgttcctta ttttcatttg
    11041 aaagctctga catgcttact cacctccgta ataatacatt gcttttggtg attccgctgt
    11101 ccctagattc tcaagaacgg ataagatctg actagtctca gaaggcttca atccctggaa
    11161 ttctgctagt ttacttccta accatgtact agcaaaccca gatggcgtgg aagaatattc
    11221 tactagttcc gcatcttcaa gctgttgttc ctttcgtata gcttttaaag catcttcagg
    11281 aaatcccaat tcatcgacta aacctacttc ttttgcttgg acaccatcat aaatccttcc
    11341 atcagcaatt tttttgactt cttcttcaga tttattccgt ccctcgctga cgattgctac
    11401 aaaacgacta tatgcgctgt cgatatatgc ttgaagcacc gcttcttctt cttttgtctc
    11461 tggtcgcgtt cctgaaccca tatctttcaa cgctccgctt ttaactgttg tatcttccac
    11521 acccagtttt tctagaagtc ctgaataatt caatcctgac atgatcacac caattgagcc
    11581 ggtcactgtt tcttctgttg caaagatttt atctgcttga gcagaaatat agtagccacc
    11641 gctagctgcc atgtttttca tgctgacata cattgggata tcatgttctt tacgaatcgt
    11701 atccaatttc ttagcgattt ccgcactttc gtagacaccg ccaccaggag aatttacttc
    11761 taaaaagata cctttgactg ttggatcttc ttcaatcgcc ttcagttgtt ccataaaatt
    11821 tttatgattg taaccttcag aagcaaataa acctgaagac cctcccgcag caatcgttcc
    11881 atctactgat aattttacga ttttttgcgt actgtctccc tcttccaaca catttggagc
    11941 tagttcttca tccccatata accaactgtt caatccctca atttgatcgt tttcctgagg
    12001 cttagtcaaa ctagctgaaa ctaacgaaac cacaaataat cctgcagcaa tcagaactgc
    12061 tgcccaacgt cttttattca ttctttctcc tttctaatac aagtttttta tgacgatcac
    12121 agtttctgac tgctcagaag tgtcgaaccg ctctgctttt tcttcccgcg catacccgga
    12181 aaacgactct tttgttaatt catcttctac cgtaaactca cgagcttgag caacttccaa
    12241 ggcatcaggt gcgatttctt ggatgctatc ccagccagta tctaaaaata ttttcgtatc
    12301 atctgataca ccagctgatg cttcgaattt ggacaattgt ttttttaaca ttcccagtgt
    12361 catgatctca atttttgaat ccatttttac tcctcctcta tccgattact ttctattgta
    12421 ccgaactcct gaaaaagatg atagataaaa acccattatt ttttgacata aaaaggacat
    12481 ttgacaaaga atttgatata taccaattta ttttttattc tttttttgtt gacaagaaca
    12541 ttcgcaaaag gttatgattt taaatgtttc cagataaaaa acaaaaagga ggatagttat
    12601 accttttagg atagcgccag gtctgtgtaa acagatgacg aggagagggt ttatcgaaag
    12661 attcggcgga tggccctagg gactgcactc tacagaaaat ccacaaaaaa tgtctgcaaa
    12721 gataaaacaa aaggattttc ctgcagctgg aagcattcat acgaattcat tatagaaagt
    12781 aggatgtttt ttatgtgcgg aattgttgga atgattggat taaaaaatgt aacaccagga
    12841 ttgattgacg gtctagaaaa attagaatac cgtggttatg attcagctgg tatctttgtc
    12901 tcagatggca caactgatta tctggtcaaa gcacaaggca gaattcaaaa tttgaaaaac
    12961 aaaataaata ttgatactac aggaaatata ggtattggtc atacacgatg ggcaactcac
    13021 gggcaacctt ctgaagaaaa cgcccatcca catacttccc aaagtggacg cttcgtgctc
    13081 gttcacaacg gcgtaatcga aaactttgaa gaacttaaaa ttgcttattt agcaaatgac
    13141 cactttattg gtgagaccga tactgagatt atcgctcatc taattgaaac tttcgcgaag
    13201 gatactacaa cgcaagaggc gtttttaaaa gctttgcggg tcatcaaagg ttcttacgct
    13261 ttcgctttga ttgatcggac agcacctgat gttatttacg tcgctaaaaa taaaagtcca
    13321 ctattaatcg gactcggcga tggattcaat gtgattgcca gcgatgcaat ggctatgctt
    13381 gctcatacga aggaattcgt tgagattgaa gatgaagaga tggttaccgt cacatcagaa
    13441 aaagttatta ttcaaaattt tgcaggtgac atcgtagaac gttcttcatt cgaagcacaa
    13501 gtagatgctt cagatatcga gaaaggtacc tatccttttt atatgctgaa agaaatcgac
    13561 gaacagccta ttatcatgcg tcgtatcgct caaaaatacg taacagaaga caatcgtgtc
    13621 tctcttgatc aaaagctggt cgatacattg tctgatagcg atcgaatcta tatcgttgct
    13681 tgcggaacta gttaccatgc ggggttagca ggtaaacaga ctttggaaaa attgacacag
    13741 attcctgttg aggttcattt agccagtgaa tttggctata acacacctct tctttcaaaa
    13801 aaaccattct ttattttctt gagtcaaagt ggcgaaacag cagacagccg ccaagtgctg
    13861 gtaaaaatca accgtctcgg ctacccttca cttacgatta caaatgttgc tggttctact
    13921 ctttcgcggg aagcatcctt tacactgcta ttgcatgctg gacccgaaat cgcagtagca
    13981 tcaacgaaag cttatactgc acaaatagct gtactaactt tattagccaa agctatcggt
    14041 gataaaaaag aatttgaaac ttctcttgta ttcgatatca ctcatgaatt gagtttagtt
    14101 gctaatgcga tggaatcggt catcgcccaa aaagattatt tagaagagtt ggcagaagta
    14161 tatctcagcg atacaagaaa tgccttttac attggacgtg gtgctgacta caacgtttct
    14221 cttgaagctg ctttgaaatt gaaagaaatc agttatatcc aggcagaagg ttttgccgct
    14281 ggcgaattga aacatggaac gattgctttg atcgaagaag gaacaccagt gatcggaatc
    14341 atcagtgaag aagtgactgg tgcgcatact cgaggaaacc tgaaagaagt cgaaagccgt
    14401 ggcgctaaaa cactagttat tgtctcagag ggattagaaa aggaaagtga ccagctggtg
    14461 ctaccaactg tccaccctta tctaactact ctaaccacag tcgtaccgac tcaattactt
    14521 gcatattacg caacgctatt acggggattc gacgttgaca aaccacgtaa tttagcgaaa
    14581 tcagtgacag tagaataata aaagaacaag gaccagtgtc agacatctga cactggtcct
    14641 tgttctttta ttttttatga aaatatctct taatccagtc cgatttcttc tttgactact
    14701 gccgcgattt tatcgacata gtaattaact ttctcatcag taggcgcttc tgccatcact
    14761 cgaagtaatg gttctgtacc ggaaggacga actaggattc ttccttctga tcccatttca
    14821 gcttctgcct cttcaatcac ttttttgatt gccggaacat ccatcgcacc gtttttatca
    14881 cttacgcgga tattcactaa tttttgcgga tagattgtta cctctgctgc aagctctgat
    14941 aatttcttac ctgtctgttt catgatattc agtaactgaa ttcctgaaag catcccatct
    15001 ccagttgtgt taaaatctaa gaaaatcatg tgcccagact gctcgccgcc aaagttataa
    15061 tcgtttttgc gcatttcttc tacaacgtaa cgatcaccaa cttgtgtaac gacatctttc
    15121 aaaccgattc cttccaccgc tttgtggaat cctaagttac tcatgacagt cgtaacgatc
    15181 gtatcttttt tcaatcgatt tttttctgcc aagtatttag cacagataaa catgatttta
    15241 tcaccgtcga caatgtttcc aagttcatcc accgcaataa tacgatcgcc atcgccatcg
    15301 aaagctagtc cagcatctgc tcctttttca acgaccatct cagctaaacg ttctggatgg
    15361 gtagagccta cgccgtcatt gatatttaac ccgtctggat ttgtgcccat tgtataaaaa
    15421 tctgtttcaa gatcggcaaa caaacgatta acacttgttg aagttgctcc gtttgctgca
    15481 tcgacacaaa tggtcaaacc ggaaaggtca ccgttaatcg tttgtacaag gaattgagaa
    15541 tatttcaata agccttctgg aaattcttct acagttccca atccttcagc tgatggacga
    15601 ggcaattcat ccacttcagc atctaataat gcttcaattt ctagctcttg gtcatctact
    15661 agtttaaaac catctgcacc gaaaaatttg attccattat cttgtgctgg attatgagaa
    15721 gcagaaatca tgacccctgc gcttgctttt tgcaaacgtg tcagataagc aacacctggt
    15781 gtagagatca cacctagctg gaacacttcg atacccaccg ataaaagtcc tgaaatcagc
    15841 gcttcttcta acatttgacc tgagattctt gtatcccgcc ctactaacac gcgtgggcgt
    15901 tttccttctt cttcatgctg gcttaggaca taacctccgc accgtcccag cttaaatgca
    15961 agttctggcg taagttcctt gttggcttca ccgcgtacgc cgtctgttcc aaaatattta
    16021 cccatagttg taaaattact cctttaactt aattttctga ggaatttgtt ctgctcattt
    16081 gtgttgattc gcttgtttct gatgtttctt ctgtacttgt ggtcgagctg cttgtcccag
    16141 attccgaact ggaagaaatg gttgttgtcg atggttccgt tcctgaatca gcagatgaaa
    16201 tacttctact cgtacttgta ttttcttgtg tccgactgct cgtcgaagca gagctactgc
    16261 ttgacgtttg caacacgggt gtaatctcca cactaactga ttcgggcttg atacttaacc
    16321 cctcttctac tggtatttca attttacgag ttgttcgatg attgatacct gagacattga
    16381 tcggtacagc aatactgtca ccgatcgatg caagcaaatc gctagagccg cttacttctg
    16441 cttccagttt tgaaaaactg aaatcatagc tttctacccc ggcagctggt gttccttgct
    16501 gtatcccata aagtcgtacc gacttgcttg gcttgatcga atcagctctc actgttactt
    16561 gcactggatc agagacgata cttaatactt cccctgctgc attcagcgcc tgtattggga
    16621 cctcactatt aatcccatca tctgtgacca tcgaagcgtc cacatcagct actactcgtt
    16681 tgatttctgc caatgttttg tcacctgtag tgatagagac ttttttagga ctaacggaag
    16741 tatcatcaag ttcatatcca gatggtgtga acgttgaagg aatcaacgtt tccacatcga
    16801 aatcttttat tacctttttc tcaaccgtta ctgtaatagt cgatggttcg atctttgctg
    16861 tgacagcact gctcaaattt tgaattttta gcggaacttc atgtgttcct tcgcccagct
    16921 tagtgatatc tgctgtgacg cggaacatcc gtgtatcttc gtttgcctct gcattcaact
    16981 gaactcggtt tgcactggaa agtttgacag tcactgcatt ttcatatcca tggatatagt
    17041 agcgctttga atcataagaa atcttgatcg gcacccccgt aactgtttga ttatacgctt
    17101 catttccaga aagcgtattt ggaacggact ggccatttgc attgaagtac agcgcaatac
    17161 tgaacagcaa tgcaataaaa ctgtaaagaa tgcttgtccg tttctctttt gagatcattt
    17221 gcgatcccct cccttagtca taccgtctag gaaagattga atcacgtttt tcttgttttt
    17281 tgttttctct tcagcaatta gttctctacg caagattgcc aaatattctt gctgggataa
    17341 gccagccatc agttcattat ctaacgtgat gctgacgtcc ccggtttctt cagagacaac
    17401 gatggtaatc gcgtcactga cttcgcttat accgacagct gcccggtgtc gagtcccgaa
    17461 ttctttaggg atcaacatac tttctgatag aggaagatag gcgcttgcta ctgcgatctt
    17521 tccttctttt acaataactg caccgtcatg cagcggtgta ttaggaataa agatattgat
    17581 cagcagctcc ccagtgatat ctgcatctaa agcaatccct gtctcaatat actcatctaa
    17641 tccagtatga cgttcgatag tgatcaacgc accgattttt cgcttggaca tatattggat
    17701 ggctttgtca aaagacaaga tcatcttctc atcttcctgc tgttcgcttt ttgattgttt
    17761 gaaaagcgaa ctgcgtccaa gatgttccag tcctctccgg atttccggct gaaaaatcac
    17821 gatagcagct atcaccccgt acgtaatgac ttggttcatc agccaagaca aggtgtgcag
    17881 accaatgatt tccgctacga ttcggataac aataaaaata gctacacctt tcagaagctg
    17941 aatcgctttt gtgcctcgca gcagttggat cagtttgtat acaagatacc agacaaccag
    18001 aatatcaagt atgttcacca aaaaattcag agagaacaag tcaccggaaa acatttgctg
    18061 ccaatagtcc aaattaaaaa gttccccgat ttgaaatgac ataaaaagcc tctcttctct
    18121 tttgagattt cctctctagt atatcatatg ttttaatttt agaggctgat atgctcgtat
    18181 ttttcttgag aaaaacgcat aattttcaag aaaattgcaa gtatgcgttt tatttttctc
    18241 tgcaataaat atttttaaac ttcagagaat tattcattat attttttctg ttatttttcc
    18301 tttttttctt ttgtagcttg ttccattttt ttacgtcgtt ctgctctggc agctcttcta
    18361 gcagctcttc tttcttctgc ttcaaggtcc gtcgtttttt cttttctagt cttaacgggt
    18421 acatcgatag attcctctgg ttcaagtcgt ttcttgattt tttctaattc tcccgtcagt
    18481 gcagcatagt tgtaaaaacg tgttttagcg tcgtggacag tcttttcaaa taaagctttt
    18541 gcaagatttg gatttgcgtt atctagcgag gaaaagcgaa cttgtttcct catgaaatca
    18601 ggcatagcag agaaatcagg ttttttgtag tccaaagtca ttgggttctt gtttttttcg
    18661 accattgtag ggttatatcg atataatgac caataaccag aagaaacagc ttcttttgct
    18721 tctttaagcg attctttcat tccgccaagc aagccatgtg tgatacaagg cgtataggca
    18781 ataatcagag aaggaccagg atacttttct gcttcttcaa atgccttgat cgtttgcatt
    18841 tgattggcac ctgaagcaat ttgcgccaca taaacatttc catatgtcat cgccatcatt
    18901 cctagatctt tctttgaagc atactttcca ctagccgaaa atttagcgat tgccgaagct
    18961 ggtgttgctt ttgaggtttg accgccagta ttggaataga cttcgttatc caaaacaagg
    19021 atattcacgt ctgctccgct ggcaagtaca tgatcgatcc cgccaaaacc aatgtcgtaa
    19081 gcccatccgt ctccaccaat catccattgg ctgtttttta cgaatagatc ctttctcttg
    19141 tatatatcct ccagttcagg gaaaattgat cgttcgcttt cgatcgcagc ttgcagtttt
    19201 gctgctcttt gctgcgttcc ttcactctca aacatgtgta ctaaccagtc ttccatcaat
    19261 tctcgtagtt cttctgatac ttttggcaat acttctttga ttgaacttgc caaagcctcg
    19321 cgacgagtct gtgccgctag aagcatacca taaccaaatt cagcattatc ttccaataaa
    19381 gaatttgacc atgcaggccc ttgaccacat tcattggtcg tataaggaga agcaggagaa
    19441 gcaccgcccc aaatagaaga acagcctgtc gcattcgcga tcatcattcg atcaccgaac
    19501 atttgtgtca ataattttac ataaggtgtt tcgccacagc ctgagcaagc gcccgagaat
    19561 tcaagaagcg gttgattgaa ttgtgtagac aagacagtat tctttccttt gactggattt
    19621 gctttttgtc ttaatgtcat tgcaaaagcc caattcacag cttgttcttt ctgttcttca
    19681 taaggcttca tcactaaagc tttttctttt gcgggacaag cctctacaca cagaccacaa
    19741 cctgtacagt cttccactga aacttggata cggtatttta cgccgtctgc gcctcgcatt
    19801 tctctcacga taaatccttc tggcgcttct tccatttcct cttcatctgc taaaaatgga
    19861 cggatggcag catgaggaca aacaaaggca cattcattac acatcgtaca gcgatcacta
    19921 atccactcag gtacttctaa agcgacgccc cgcttttcca tagaagccgt tcctaaaggc
    19981 atttcgccag ttgtcattcc atttgctaca agtgtaccaa cagagagttg atttccttcc
    20041 tgtgcattca ctggttcaag tatttcttgt atataaccag tgttcctttt attgattttt
    20101 tcaggcatct ccaacgttct ccaatggtct gggatcgcga cttgatgaag cagttcggtc
    20161 gtccgatcga ttgcttgtat gtttttttct acgatagcca ttgatttatg tccatagctt
    20221 ttcagggctt cttcttttag cagcggaagc acctcatcaa aaggaatgat gtcagctaat
    20281 ttaaaaaaag ccgtttccat tgccgtattg atccggcgtc ccaaaccgac ttcttgggcc
    20341 aatctcatgg cattgatcgt atagaatcta atgttatttt cagccaagta acgttttaat
    20401 tttaagggca gatgcgtttc taattgttcg tcgctccaaa gagtattcaa taaaaaaatg
    20461 ccacctggct tcaatccttt gaccaagtca tagctatgca ggtaagctgg tgtgtgacag
    20521 gcaacaaggt ccgcatgctc gacaagatac gctgaacgta tcggcgtatc tccaaaacga
    20581 agatgagaga cagtcagacc gccagatttt ttcgaatcat aataaaaata gccttgcgca
    20641 tatttatcgg tgtgatctcc aataatcttg atagctgatt tatttgctcc aactgttccg
    20701 tcagatccaa atccccaaaa cttggcctgg aatgtttgcg gttctgtcaa atccagactt
    20761 tcctttggtt ccaaagattg ataagtcaca tcatccacga tccctatggt aaaccgtttt
    20821 ttgcgtatgc ttgggtcttt tttcaattca tcaaaaactg ctgagatttg gtcgggagtc
    20881 acatctttag accccaatcc gtagcgacca ccaataacag ccgggcgaag ttcagaatca
    20941 tataacgcac tttgaacgtc taataagaga ggttctccac ctgcccctgg ttctttactt
    21001 cgatcaagaa cagcaattgt ttttactgta tgaggcaact tttgcacgaa tgtttccact
    21061 ggaaaaggac gataaagatg gatattcaaa aaacctgttt tccgtccgtt tgcattcaag
    21121 taatcaatcg tttgttcaat cgtctgtgca actgatccca tagaaacaat gacttcttcc
    21181 gcatcgtctg ctccgtaata agtcaccaag tcataagaag ttcctcgtaa tcggttgatc
    21241 tctttcatat atttttgaac gatttcaggt aatttatcat aataggggtt gatcgtttcc
    21301 cgttgttgaa aatggatgtc tggattctgg ttcgtaccac taacagtcgg atgattggga
    21361 ttcatccctc gtttacgaaa tgaagctaat ttctcctgat tgatcagagg agctaattct
    21421 tcatattcca aaacttcgat tttttgaatc tcgtgactcg tacggaatcc atcaaagaaa
    21481 ttcaaaaaag ggacttctcc ttcaatagaa gcaagatggg caacaggtgc taaatccatc
    21541 acttcttgta cactgctttc agctaacata gcaaatcctg tttgtcttgc agccatcacg
    21601 tctccgtgat ctccgaaaat attcaacgca tttgtcgtca ctgcccgact agcaacgtgg
    21661 aaaacacttg gcagtagttc cccagcaatc ttgtacatat tcggtatcat caaaagcagc
    21721 ccttgtgaag cggtatatgt agttgtcagt gcaccagttt tcaatgatcc atggaccaca
    21781 ccagctgctc ctgcttcgga ttgcatttct actactttta ctggttgacc gaatacgttt
    21841 tcttttcctg ctgctgacca ttgatctact aattcagcca tggtcgaact gggtgttatt
    21901 ggatatattg cagcaatctc ggtaaatgca taagaaatat atgcggctgc cgtattgcca
    21961 tccattgttt tcatttttcg catgattcat catccttgtt catattctct cttattatct
    22021 gttccattat aacgtaaaac atgccatttt tttcttttat ttataaaaat attttacatg
    22081 tatctatttg aattggtcat ctaattttta gacaaacagt ctaaatcgtt gccttttttt
    22141 gtcagacatg tataaactat aagcgaaaga ggtgttttaa catggatata tcagtgattg
    22201 atgcaacgaa aacaaataca caaaaaggaa tcctttatgg ttcatcgaat gctcctaaaa
    22261 aaatggtcga attcatcaac ttagcttgtc cgtattgccg acaatggttt gaagaatctt
    22321 atgagctatt agaagaagca gtccaaagcg ggcaattgca gcgagtgatc aaattgttcg
    22381 ataaagaaaa agaaagctta cagcgaggga acgtgatgca ccggtatctc acaatcagcg
    22441 atggccaaaa agcgatcaaa gagatcaaac aaatattcga tactcaagac gagtggaaac
    22501 atctttctct ccaagaagta gcggattttg cagtagataa actaaagctg acagaacaaa
    22561 aagacgaaca gctgagccaa gctgtcatca acgaagctga gcaagcacat attcgatttg
    22621 ttccaactgt catcttgggt aaagaaatct ttgatgaatc tatttcaata gaggaattaa
    22681 aagaattgat ccaaacaaaa taaaaaaata aggagcgtg
//
LOCUS       DAZKLA010000036        22478 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000036 DAZKLA010000000
VERSION     DAZKLA010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.036"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(223..675)
                     /locus_tag="ACG85N_001176"
     CDS             complement(223..675)
                     /locus_tag="ACG85N_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0223960.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(688..1098)
                     /locus_tag="ACG85N_001177"
     CDS             complement(688..1098)
                     /locus_tag="ACG85N_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0223961.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1373..1585
                     /locus_tag="ACG85N_001178"
     CDS             1373..1585
                     /locus_tag="ACG85N_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFQ0223962.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1588..1800
                     /locus_tag="ACG85N_001179"
     CDS             1588..1800
                     /locus_tag="ACG85N_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223963.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            1940..2095
                     /locus_tag="ACG85N_001180"
     CDS             1940..2095
                     /locus_tag="ACG85N_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223964.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2092..2316)
                     /locus_tag="ACG85N_001181"
     CDS             complement(2092..2316)
                     /locus_tag="ACG85N_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0223965.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2394..2582
                     /locus_tag="ACG85N_001182"
     CDS             2394..2582
                     /locus_tag="ACG85N_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223966.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2579..2752
                     /locus_tag="ACG85N_001183"
     CDS             2579..2752
                     /locus_tag="ACG85N_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223967.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            2721..2849
                     /locus_tag="ACG85N_001184"
     CDS             2721..2849
                     /locus_tag="ACG85N_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223968.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            2908..3243
                     /locus_tag="ACG85N_001185"
     CDS             2908..3243
                     /locus_tag="ACG85N_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223969.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3476..4417
                     /locus_tag="ACG85N_001186"
     CDS             3476..4417
                     /locus_tag="ACG85N_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFQ0223970.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4419..5309
                     /locus_tag="ACG85N_001187"
     CDS             4419..5309
                     /locus_tag="ACG85N_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFQ0223971.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5352..6152
                     /locus_tag="ACG85N_001188"
     CDS             5352..6152
                     /locus_tag="ACG85N_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFQ0223972.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6167..7018
                     /locus_tag="ACG85N_001189"
     CDS             6167..7018
                     /locus_tag="ACG85N_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0223973.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7219..7680
                     /locus_tag="ACG85N_001190"
     CDS             7219..7680
                     /locus_tag="ACG85N_001190"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0223974.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7706..8158
                     /locus_tag="ACG85N_001191"
     CDS             7706..8158
                     /locus_tag="ACG85N_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223975.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8264..8458
                     /locus_tag="ACG85N_001192"
     CDS             8264..8458
                     /locus_tag="ACG85N_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223976.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8489..8782
                     /locus_tag="ACG85N_001193"
     CDS             8489..8782
                     /locus_tag="ACG85N_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223977.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            8713..9252
                     /locus_tag="ACG85N_001194"
     CDS             8713..9252
                     /locus_tag="ACG85N_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0223978.1"
                     /translation="MVSEKQREFSSGEIGGHGSYKILILLFYNYPTTMSVIPQLEGEE
                     AIVNILENIDIKQTRKNARRLLKRYRNLERLVGPVKIDFSVMTVTKNLKFTIDSQNEE
                     IIEAISTRDSVIEALTRLSRIHFQVLYYSYCFPNKMSMYQIGEKLGYSDRTIERMKAV
                     ALVEFAEAYKSGELISRTK"
     gene            9327..9794
                     /locus_tag="ACG85N_001195"
     CDS             9327..9794
                     /locus_tag="ACG85N_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFQ0223979.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            10541..10771
                     /locus_tag="ACG85N_001196"
     CDS             10541..10771
                     /locus_tag="ACG85N_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223980.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            10930..11763
                     /gene="terS"
                     /locus_tag="ACG85N_001197"
     CDS             10930..11763
                     /gene="terS"
                     /locus_tag="ACG85N_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFQ0223981.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11756..13171
                     /locus_tag="ACG85N_001198"
     CDS             11756..13171
                     /locus_tag="ACG85N_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0223982.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13183..14712
                     /locus_tag="ACG85N_001199"
     CDS             13183..14712
                     /locus_tag="ACG85N_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0223983.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14630..15682
                     /locus_tag="ACG85N_001200"
     CDS             14630..15682
                     /locus_tag="ACG85N_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFQ0223984.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15684..16001
                     /locus_tag="ACG85N_001201"
     CDS             15684..16001
                     /locus_tag="ACG85N_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223985.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16128..16751
                     /locus_tag="ACG85N_001202"
     CDS             16128..16751
                     /locus_tag="ACG85N_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFQ0223986.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16766..17656
                     /locus_tag="ACG85N_001203"
     CDS             16766..17656
                     /locus_tag="ACG85N_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFQ0223987.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17679..17894
                     /locus_tag="ACG85N_001204"
     CDS             17679..17894
                     /locus_tag="ACG85N_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223988.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            17906..18247
                     /locus_tag="ACG85N_001205"
     CDS             17906..18247
                     /locus_tag="ACG85N_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223989.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18247..18618
                     /locus_tag="ACG85N_001206"
     CDS             18247..18618
                     /locus_tag="ACG85N_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223990.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18611..19006
                     /locus_tag="ACG85N_001207"
     CDS             18611..19006
                     /locus_tag="ACG85N_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFQ0223991.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19008..19385
                     /locus_tag="ACG85N_001208"
     CDS             19008..19385
                     /locus_tag="ACG85N_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFQ0223992.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19386..19994
                     /locus_tag="ACG85N_001209"
     CDS             19386..19994
                     /locus_tag="ACG85N_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFQ0223993.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            19994..20335
                     /locus_tag="ACG85N_001210"
     CDS             19994..20335
                     /locus_tag="ACG85N_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0223994.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20550..>22478
                     /locus_tag="ACG85N_001211"
     CDS             20550..>22478
                     /locus_tag="ACG85N_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFQ0223995.1"
                     /translation="MNNDDLILKMILDESGFTAGMNNAVKKLNSFDETIERKSRNSGS
                     SLGSIWKIFAGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNS
                     EIMKNIGKNGLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFG
                     GSTDQVNEAVIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLS
                     QGKISVEEFQNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIE
                     GLNKALVDSDLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALK
                     FMMPFLVPAATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVL
                     IIGAVVGAIAVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEM
                     ASNAVEGVKDAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKW
                     FSDTWQSIKDSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKN
                     VFEILKNVILAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFT
                     NIYFAALNIWTGFKLTLIN"
BASE COUNT         8063 a         3303 c         4611 g         6501 t
ORIGIN      
        1 tctatcatgt aatttattta gtttttcatc atttttttgt tctaattcat aagcttcttt
       61 ttttattgtg ttagctttcg acagtttagt gattcctata attaataaaa taagtccaat
      121 caataataac attcaaaact cctcatttct ttgatataat tattgtagag tttactcaga
      181 aatgaggaaa gagtccatgt gaaagcatgg gcttttttat ttttaatgtt gtaatactaa
      241 atgttcaaga ttatgaggaa gccctaaata ttcaataatt tggaactgac tcatatcaga
      301 taaatcttgt tcatctattg ttctagttaa catttcaatt gcaaacatat tagcttcagc
      361 ttctatacca taaatcatac aactaaggtt gtttgctcta aaagctgctg tgcaaatacc
      421 tttatggagt ctaacgtggg ctatttcatg ccataataca aatcttttca tagaaggtga
      481 taggttttga ttaattaata tcacataaca tcttttattg tgtatcgttt gtcctaatgt
      541 tttattccct aaatcagttt caattaaatc tatatcagaa tatttgatta ttttatatgg
      601 attaatcgca tcagatccaa actctacaaa taatttttct atttcttcta atatccagta
      661 tttgtttaaa tgcataccaa agcccaccta ttgacgatat tttttaggag tgaatttttt
      721 ctttgcttta actttggtta aggcaagtgc aagttgaatg gcatcgtata atttttcctt
      781 atcttcatcg tctaaaggtt caccataata atttgtttca gcatctgaat tcaagccttc
      841 aagtaatttt tcagcttgta aagcaatatc ttgtttatct ttttctgtta aatcataata
      901 atggcgttta tcagtacgtc ctaaaagata atcagttgat acattaaagt aatctgcaac
      961 tttttgtatt ttatctccag atggagtact tttattccat ctagacatgc ttccatttcc
     1021 gaaatttaac tttctttcaa gttctgccaa cgtcattgaa cgttctttta ataagatttt
     1081 tatacggtct aatagcatag gatatgcatt cctttcaaaa taccgacaaa aaaagtattt
     1141 ttctgcaaat tatctattta caaatagaga atttgcagtt atactagttt tgtaagcaag
     1201 atatttacgg catacaaaga ctaaaagcaa taaactaata gtctccccag acatcagtaa
     1261 gctcgctttt tgtgcgctgt attttgtatg cttacataat agcatatttg cagtttttgt
     1321 caaacttatt agacaaaaat attagaaaat attcgataag ggggtatgaa gcatgacttt
     1381 aaaacagcaa gtattaattg ctttagtaaa gaaaggttgg agtcaaagag agttagctcg
     1441 acgaatgggt atatcaatta cttatttacg agatatcttc atcgaaaaaa gaaaacctaa
     1501 ggaacgttta aaacaaatcg aagaactttt ggatatcaag ttagaagtag attctaatga
     1561 tcagccatca gaacaggagg cgtaaaaatg gtacaacgtt tgagcaaagc ggagtttaaa
     1621 gagaaatata agtattctga aagtacttat caaagaagaa tgaaggaatt taaaaaatca
     1681 cgttttagtg aggggtataa cgcagttact tccaaagaaa taacaattga tgtcgattta
     1741 tatgaacagt ttagaacatg gcgttctaaa aatcgattta gagttagagc tgtttgttaa
     1801 tagctaaaaa atctaaattc accacttctg aaaagaggag ttgctgctct cctttcaagt
     1861 ttgccaaagt ccacgttttt tcattttggc aactcctctt cttggaggtg gtgacggcga
     1921 gagacgaagg agggatgaca tgaaggaaga atcaaaaata gggattttga atgagaacaa
     1981 ttacacaaga ttaattattg aaacagaaga agggaaaaaa gtagcagaag ttacattgac
     2041 cgatgcaact cctgctgacg gttatcgtat tcgattaact cctaaatatg attaaccttt
     2101 tggtgggtgt ggatcgtttc cgtgactatc acggctttga atttttccat ctctgccatg
     2161 aataaatagc tcagatcctt gatttttaga aatctctcta gcgattttta ctgcttctga
     2221 ttttgtatta gtatgtgcag tcgctttaga atttccagca cctttgacat tccaaccacc
     2281 attggaatta ggtacaacat gttgattttt tcccatagtt ttcacctcca tttctataga
     2341 aatattttat caaatagaaa tggagaaatt caataaacga aaggagaagt gatatggcat
     2401 ataccttgca acaagaacat caaattctcg gtttgattaa acaacgcagg aaacaattac
     2461 aagatgaccg tgcagcgctt agaaaagccg atgagctatc agataggcaa gctgaactaa
     2521 ttgcttctga acttgaggat ttgagaatgc tagaaataaa aaatagggag attagattat
     2581 gaagaagaca gacacacttt ttataggatt cattttgggg ttattagtga ttgtagcgca
     2641 ccaaagtatt atcgggggaa gcttgttcgc agcattgatg gttttaatca atctgcttga
     2701 ttcaaaagaa aggagcaact atggcacgag aagaagcgct aaaaatcggt aaagtgattg
     2761 ctgataatcg gtggggaaat agccgcccta ttattttaag caagcaacat atcgaaaagc
     2821 aaaaagcatg gcaacaaata aaaaagtgac tctgccagcc agcaaagagt cacatacaaa
     2881 tttataaaac taggagaatt atatcacatg aataagaaaa ttgaaaatct tatcgaagaa
     2941 ttgaaacgtg aatgccaaaa gcaaggtgtt tctattattt gtacagccca aaaagaaggg
     3001 gaacttaaaa gtctcgttta tggtgagaca acagaaattt tactttgtct tgctatgcaa
     3061 gaagaacatt tagatgaaaa tctcccattg tcagcacata ttatgcgtag aattgctgtg
     3121 gatgcttacg aacaagcgaa aaatgaagaa gaaaatcaac cttcaaatca tacttttgtt
     3181 attaataaca aggaagattt agctgatgtg atgactcgta ttctcaaggg ggaatttcaa
     3241 tgatgaatga aaaaacaatt gatctactca ttattttatt tttcacgtta gtgattgtag
     3301 cgaaaagtta tgtaatcttt ggcttgttat tcgcaattac catgcttgtg ctttgcgtgt
     3361 tagcaagcag aaaagaggtt aaacatgaac gagttagcaa atcttgacaa ctatttaact
     3421 gatcctgatt atgctaaacc accatatgaa gcaccaattg atgaggagga tgaagatgag
     3481 taaatctacc ttagaaatga gccatcaaga atggcttgaa gaccgtaaga aaggcatcgg
     3541 aggttcggat gttggaacaa ttttaggatt gaataaatgg aaatctcctt atcaactatg
     3601 gctagaaaaa acaggacaag tagtacttga agaatcagga agtgagcccg cttattgggg
     3661 taatgtttta gaagaagtgg ttgctaaaga gtttcaggaa cgtacaggca aaaaagtacg
     3721 cagaagaaac caagtatttg aacatccatt acatccattt ttaagagcaa atattgatcg
     3781 ggaagtagtg ggagaaaatg ccattctgga atgcaaaaca gccaatcaat ttctcggtaa
     3841 agaatgggaa ggcgaagagg taccgcttag ctatctctgt caagttcaac attacatgaa
     3901 cgttctaaac aaagactatt gttacatcgc tgtcttaatc ggtggtcaaa aatttatctg
     3961 gaagcggatt gaacgagatc aagagctgat cgatacaatc actgaacaat tagtagagtt
     4021 ttgggaaaca aacgttcttg gaggtatcga gccaattatt gatggtagtc aagcgacagc
     4081 tgatttcttg aaagagaagt atgcagatgt tgaagacgtt caaacgacgt taccaattca
     4141 tttcgatgaa ctagttgaac agaaaaacga actcaaacgg actaagaaag aaattgaatc
     4201 agctatccgt caagtagaca acgagattat cagcgagcta ggtaaacgtg aagccagtat
     4261 cggtatcact caaaaaaaca tcatcagttg gaaatttgtc cgtacgagac gtatgaactc
     4321 gaagaaacta gcagagaaat atccagatgt cgcaaatgat gaagagattt ataacgttac
     4381 tgaatcaaga aggctaaccg aaagggagat caaataatat ggcaacaaat gaatcgttaa
     4441 aaaatcaatt ggcagcaaag ccacagaaac aagttgcacc aggtcaatta ggacttaaag
     4501 ctttgatgaa tacaccaaca atgagaaaga agttcgaaga ggtgcttcat gacaatgcta
     4561 atgcttttat gtcgaatgtt atgactcttg tatctaatga cagttatctt gcagatagtg
     4621 aaccgatgtc tatcatgagt ggtgcgttaa ctgctgcaac attaaatctt gggctagata
     4681 agaatttagg ttatgcatat ttagttccat tcaatagtaa aaacaagcaa acaggaaaat
     4741 gggaaaagaa agctcaattc atgcttggct ataaaggata tatccaatta gcccaacgat
     4801 caggtaaata caaagcatta aatgtgatcg aagtttacga aggagaacta aaaagctgga
     4861 accgactgac agaagagttt gagtttgatc caaatggtag aacgtctgat gaagtcattg
     4921 gatatgttgg ctatttcgag ttactgaatg gattcaaaaa aactgtctat tggaccaaac
     4981 aagaaatcga agctcatcgg attgctaaca ataaagatcg cgataagaca aagttaagtg
     5041 gtgtgtgggc atctgattac aatgcaatgg cacgaaaaac tgttttgaga aatcttcttt
     5101 ctaaatgggg aatcttgtcc atcgaaatgc aagaagccac cacatcggat gagagagtcc
     5161 aaagggttca agaagacggc agcattattg ctgaaacaga agttgaagaa gatattcctg
     5221 aaagaaaaga agcagaggtt atatctgaag aaaatgaaga tgtacaaact ggattatttg
     5281 atgcatctaa tccgccgtta aacaaataat gagggagctt tctcccttgc ttttctagag
     5341 gagaaatacg aatgaacaca ggatatgtca aattacatcg aaaagtgatg aattcattcg
     5401 tttggaccaa tccttatatg tataaattat ggaacttgtg tttgatgaaa gcgagtcatg
     5461 agaatcgcaa gatacttttt aatggaaaag agatacaagt gaacagcgga gaattcgtca
     5521 cagggcgcga tgctatcaca tctgagatga acgaaggcgt gaaacgtgaa catcaagtga
     5581 acagcggttc tgtatggaga tggctaaaac aatttgaaaa aaacggaatg ttgaacatca
     5641 aatcaactac gaaatacagc gttatatcaa taaaaaactg gtctttgtac caaagcagtg
     5701 aacaacaaat gaacatcaaa cgcacaacaa gtgaacaaca agtgcacaca aacaagaatg
     5761 aaaagaatta taagaatgaa aagaataata aaacatcatc gtcgcaacca cgcaaaaagc
     5821 gtgtttacga caccgactca gtttactaca ttctcgcgga ggagttattc aaacagattt
     5881 gtcagaatca ggaaatcaaa aagccgaatc tgcaaagttg ggcagatgac attcggaaaa
     5941 tgatcgagat cgacaagcgg accgaaaacc aggtacgggg aatgattgaa tggagtcagc
     6001 acaatgtgtt ttgggcatcg aatattttat ctgctaaaaa attgcgagaa aaatacgaca
     6061 caatggcagc gcaagcaaat cgtgattata aaacaaaaca aactaaaacg cttgaatacg
     6121 agaaatttag cacagatgag ttgcctattt gagaggaggc gtaggcatgg aaactgttgg
     6181 ggaaatcatg gaaaagctga tacagaaagt gcttgtccaa cgtggcgaat gtcctgaatg
     6241 cggacagcct ttgtatggat ggcgtacgaa gaaccctgat ggctcagaac gttgtaagcc
     6301 aacctgtatg caatgtgggt ataaagcatt gcgagttcag gaagacttac aaacagaacg
     6361 aatttacaac gagagcctga aagcaagagc aatcaatttt ttcaaaggtg gttctgttgt
     6421 gcctaatcaa gcgttgtttg attgcacatt gcagaattat caaattgtcg atcaagaaac
     6481 aagacaagcg gttgaagtaa ccaaacgctt tgttaattca gtcttgttag gaaatccaag
     6541 tcaccttgtt ttaactggaa aacaaggaac aggtaaaagt cacctagcga tggcagcggc
     6601 ttgggaagtc ttgaagcgat caaactacga taaaaaaatc ttatttatcg ggttacagga
     6661 aatgctggat caaatcaaat tttcgtacaa caatcctgaa ctcagaaaaa cgattgaggg
     6721 atcgttgatt gcagatatca aaacagcgga tttagtcatc attgatgata tcggttcaga
     6781 actaggaaaa gatgcatcag atagtcgagc gtttggcata aacacgctaa attcgttctt
     6841 ggatgcacga cagaacctag caacgattat cacaacgaac ttgcttggtg aagaactgaa
     6901 aaaagcttac ggtacgagaa caatatcaag aatgtttgtc aactctgatg gatttacgat
     6961 ggtattttct caaacagcag acaagcgcat aaaaccagtc aaaggagcga tcgcatgaat
     7021 ttgactaaac aagaaaaaag aatctgtcgt cagataatgt tgcaaattat aggtgatcgt
     7081 gacatggacg aattatctca acaagagagt cgaatggttc ggaagataag taagctaatt
     7141 atttatcggg atacagaaag atttctcact gttttaggta acgtctacga gaatccagag
     7201 ttattggagg tagagtaatt gccaaaaata ttagatgcat gttgcggtag tagattgttc
     7261 tggtttgata agaacaattc tgacgtaaca tttatggatt gtcgtcagca gtatgaagaa
     7321 ttatcaacag gtcatgtgat caatgttgat ccagatgtgg tcgcagactt tcgcgatatg
     7381 ccttttgaag atgatgtgtt cgatatggtt gtatttgacc cgccacactt aagacatgtc
     7441 ggcgaaaact catggcttgc caaaaaatat ggaaaattag acgaactttg gccagaagac
     7501 atcaggcaag gttttgcaga gtgtatgcga gttttacgac cttcaggaac attaattttt
     7561 aagtggaacg aggaacagat accactttct gatgtgttag aggctattgg agaacagcca
     7621 ttgtttggta ataaacgaag caaaacacat tggcttgtgt ttatgaaaaa attcagctaa
     7681 cggaggaaac aaaggaggaa aagtaatgaa gattgtatta aataaatgtt ttggtggctt
     7741 tgggttgagc catgaagcaa aaatggaaat ttttaaaaga aaaaatattg aagtattccc
     7801 atacatcaat aactttagct atgactcaga tgatgaatac acgagataca caggccaaaa
     7861 gctaggttcg atggatttta tctactattt caaaaaagac ccgaaaatcg acaaagttac
     7921 aggaatttat agcgaaattg accgactgta cggaattgct gatgattcca gtttttcaag
     7981 tgattcaaac agaggtgata aggacctcgt tgctgttgtc gaaaaactag gaagtgaggc
     8041 cagtggacca tatgcaagtc ttaaagtcgt tgatataccc gatggggctg agtgggaaat
     8101 aagtgattat gacggcgttg aaactgcaca ttatggtttt caaacaggta gtatttagtc
     8161 cactaatgac aactaaagag gaggcagaag gatgagtgaa gatgaatact ttgaacagta
     8221 gtctattaac gacagagatt tttaactgga aaggggtgta gtcatggacg catcggaagt
     8281 agtaaataaa attaatagtc tcaaagaggt tttcggagac gtcgaagtag tgataaacgc
     8341 acaaggctat gacaacgaat tttttaagca gattaccgac attagcattc agcaaggaat
     8401 ggaagacgaa gacggaaact ttatcgatga agtagccatt ttagtgactt gcgagtaagt
     8461 aattgacagc taaagaggag gaagcggaat gaaactaaaa gacggatttt actccagcgg
     8521 ccacggcatc ggcggtttaa tgctagatat gccgacaaag aatcctaaaa cacgtaagaa
     8581 accaaaattc aaagtcggtg acatggttcg ctgcgaagca gaaggattca tctatccatt
     8641 tcgtggatat gtagaacacg tctataatca ctcagcaatc attcgcattg aaaacacgat
     8701 ggaatgtgat aagtggttag cgaaaagcaa agagaattta gcagtggtga gattggtgga
     8761 catggaagtt ataaaatctt aattttgctt ttttacaact atcctacaac aatgagcgtt
     8821 attccacaac tggaaggaga agaggcaatt gtgaacattt tagagaatat agatattaag
     8881 caaacaagga aaaatgctag acgattacta aaaagataca gaaatttaga acgtttggtc
     8941 ggaccagtga aaatagactt ttccgtgatg actgttacca aaaatttgaa attcacaatt
     9001 gacagtcaaa acgaagaaat cattgaagcg ataagtactc gagattcggt tatcgaagca
     9061 ttaacacggc taagtagaat ccatttccag gtactttatt atagttattg ttttcctaat
     9121 aagatgtcaa tgtatcaaat aggagaaaaa ttaggatatt ccgatagaac tatcgagaga
     9181 atgaaggcag tagctttggt tgaatttgct gaggcgtata aatcaggaga actcatttca
     9241 cgtacaaaat aaaaaaagtc ggattcctcc gactatgaat aatatttccg acataagtat
     9301 tatatcataa ttgggggaat cagaggatgg tacttttcga tgtaaagaaa tatgagacac
     9361 caagcgcgaa agatgttgat atggaacgca caaaacataa tgtcgctgtg tttctttcag
     9421 catatctatc agctagatgt agagtagggc aacatcgaga accaaaagtg acagcatctt
     9481 attccttggt tccaccttct acagctgatc atatatttga agcagaaaga atgctgatcg
     9541 ataaagaaga agcacaggaa gaatttgagt atctgcataa attgtttgtt cgaggctatt
     9601 cagcgataca gcatccgcat aagcctgatg tgactgaaag gcgcaagaag atattctatg
     9661 atcgttatat caatggtctg cccatttatg taactgctca aaggaataat actagcgaag
     9721 aatcggttaa agtagaatca aacagaatta tcatccaatt tgcttcatcg ttagaactgg
     9781 ttgctttcaa gtagccagtt tttacacttt ttataccctt ttattaccaa tttggtttcc
     9841 attttatacc ttttttatac caatcactta cctattcaac gttgtattat gatagtgtcg
     9901 aaaaattagg aaacaggatc gacaaaataa aatgtaaggg aggaaatctc cctcatcgtt
     9961 taattaagct tcgataggca gcaacggaaa tattaagaat aaggatgtga atttcaactc
    10021 cttctaaatt gttcttatta tctatcatcc gttgctgtct attgtttttt taattattca
    10081 cacgataact aaaggtaagt gaagagaaat gattccatta ataatttcaa tttttgcgct
    10141 ttgtcttaat gtatatatga ttggatttaa aaatgggcaa aataaaaaat agtagcagcc
    10201 aagaataatt ttatagtgtc actgtggcgg aaagggtaga cgctaagcat gtgtgctagg
    10261 tcaatgcttc ggcaaccatg caaggttcga ttccttgcca gtgactttgg caaccgaggc
    10321 atcggcggtt taaaaatata ggggtgcgca atttcgtacg cgttttgtgc atcgtgtagg
    10381 ttgctattac ataactggtt aggttagatt gagatttggg attcggtaca aatgaatcgt
    10441 caaatgtctc aagcacagga tcggaaacgt ccctgcctgt gcattacata ttagatcact
    10501 ctttgagtgg tctttttctt tacataaagg aggctacata atgagaaact actggtatgt
    10561 atcgctaact aatgaatatc ctcgaaccat tgatgattgt tcagtgcgtg ttgtgcgttc
    10621 tgtacaaatc aaagggaagt actctattgt cgaaatgggg agagaagcag aaccatgtga
    10681 gattgatgcg tgcaagctcg tttattgcgg tcatggtttc tatgatgaac caaacattca
    10741 aaataatatt aacaagaatt tgagggatta gaatgcaaga aatggcttaa aaatctccaa
    10801 tcaggacaaa tcaagttaga caaagtttcc gatttaaaga tattgattga agctgattta
    10861 atgctaaaag atattgaaaa ttagaaaaca aaactcaacc taaacagaat gtgaggtggt
    10921 gtgtattgaa tggcaagaca acgtgatcca agacgtgatg aagccaaaag aatttggtta
    10981 gaatccaacg gagaaaagca gttaaaggaa attgcatctg aattaaatgt ttcagattct
    11041 caggttagaa aatggaaatc gcaagacaaa tggagcgctg aattgattag taacgttacc
    11101 aatggcaaaa gtaacgttac taatcaaggt ggcgctccta ttggtaatca aaatgctaaa
    11161 ggtaacaaag gaaatagccg agcttctccg ccagtgggta ataaaaacgc tttgaaaaca
    11221 ggtgagtatg aaacaatatt ttttgacacg ttaaccgata atgagaagga catctattct
    11281 agtttgaatg atgatccttc ttttgctttg tccgaagaaa tacgacttct taaaataaga
    11341 caacttcgaa tgatgaaaag aatccaacaa gctgaagctg gactaaatga tgaagaagtc
    11401 gaacgattgc agcagctaag aaagattaaa actccgattg aaaaaaatgg taaaaagcta
    11461 gaaatcaagc gtgaggttat gcaagatgtg cagattagca gaaaaaaaca tcgcaaaatt
    11521 gatgatattc tttcaattga agattcattg actcggatta gcaaccagtt agctaaagcc
    11581 atcaagcaaa tgaatgaact ttatatgaat gaatacagaa ctgatttaat taaagctcag
    11641 actgataaga tccaagctga gacaaatgaa attggcggaa ataattcagg tgaagaaata
    11701 gaagaatgga aacaggcagt tttaaatgcc gcaaacaaac gggcggtgaa agaaaatgaa
    11761 taatgaattt attccttttg ctgatattgg ttctgccatt gattattact atgataaacc
    11821 agtagctttt tgccaagata ttttgcattt gaatcctgat gaatggcaag aaaatgtttt
    11881 aaatgattta gctgaatttt caaaggtttc tgttcgttct ggtcaaggag ttggaaaaac
    11941 agcattagaa gcaggagcaa tactttggtt cttaacgtgt cgaccctacg ctaaagtaat
    12001 agcaacagct ccgacaatga agcaacttta cgatgtactt tgggcagagg tagctaaatg
    12061 gttaaatgat agcttgatca aaaacttact gaagtggaca aagaccaaaa tttatatggt
    12121 tggtgattca gagcgttggt ttgctacggc tagaacagcg actaaaccag aaagtatgca
    12181 aggttttcac gaggaccata tgttgattgt ggtagatgaa gcttctggtg tgtctgatcc
    12241 aattatggaa gctattcttg gtacgctatc aggttttgat aataagctgt tgatgtgtgg
    12301 aaaccccaat aatattgaag gtgtctttta tgattcccat aattcagacc gtgataaata
    12361 cagagttcat aaagtatcaa gctatgatag taaacgtaca aacaaagaca atatagaaat
    12421 gattcttaaa aaatatggaa aagaaagtga tgttgctcgt gtccgtattt ttggagaatt
    12481 tccgaaaggt gcgttggatt catttatcag tcttgaaaca gttgaattgg ctacagaaaa
    12541 acaaattagt gattctttag tcaataaaac aaaggttgct catattggtg tcgacgtagc
    12601 tcgatatggt gatgattcta cgattctctt tcctagaatt gctaccaggg cattggagta
    12661 tgagaagtat tcaaaacgta gcaccatgga aacaacagga tatgtcatca acatggccaa
    12721 gaatctaatg agtcaatatc cgagtattga taaagtgatg attaaagtcg atgacactgg
    12781 tgtcggaggt ggtgtaaccg accgcctaga agaacttata gaagacaaac attatccttt
    12841 tgaggtgttt ggagtgaata acggttcaac atcagaagac gatttttacg ataatttagg
    12901 tactcaacta tggggaaaca tcaaggaaat gttagaagaa aatatgacag caaatcttaa
    12961 cggagaacag cctgttattg aattgccttc tgatagttcg ttaatcaaag aattaagtac
    13021 tcgcaaattc aaaatgacaa gtagaagtcg tatacgttta gaaagtaaag atgatatgaa
    13081 aaagcgaaat attggtagtc ccgatattgc tgacgcactg gctttagcgt tttatgagcc
    13141 accaagtcac tatcaattta ttcaatttta ggaggtgagc ttttgattag tacagtatta
    13201 tctttagaga gatataagaa gctacgtgtt aaatatgcaa cacaaattga agatggaatg
    13261 tttgatccga atggttttat agaagatatg aaaccatttt ttgctgatcg cgaaagaaaa
    13321 tatctagctt atactagcga aaagaatgaa atagataata gaccaaagcc taacactgat
    13381 attgtaaaag ttaataataa acttcatgct ggtatgtatt caattgtcgt agaccaagca
    13441 gtcaatcatt ttactggtat acctatcaaa tgggattacg atgtatcgga acaaaaaaga
    13501 acactcattc aaagattaaa agataaattc ttaaaaaatg atatagaact tccgacagtt
    13561 ccagaagctt ttaacaaatt aacaagcaat cttgattcca tgagattcgc aatgcttgat
    13621 tctgaaactg caacttttca aggagcttgt ggagtagctt tccgattgct agagcctgtg
    13681 gaagaagatg atggttggaa gttaagagca agtaatattg aaccttggag agcagaaaga
    13741 tatggaaatg ccggaatcta tatcaaagaa aagtatgact cttaccagaa aaaatttttt
    13801 caagaaatga aagttatgac aagaaataaa attctgacat atgcttgcta tggtgatttg
    13861 aattttgtta caagcggaac gttcaaaaaa attgatgaga ccgataaccc tttggggacg
    13921 attaccttgg cagaatttaa aaataatacg aatcgttatt gtgattttga agtagctgag
    13981 gaaattggag atgcaattga tcgtgctttg tctgatcaac aaaacgaaat cgaacaattt
    14041 aagcttgcgt acatgcttgt tactggcaca acaatgagca aaggcactgc taaagaaatg
    14101 atgaatcagc tagggattat caacttaaaa gcccctactg caaaagctga atatgtcaca
    14161 aaaaatttag caaaagattt caatgaatat catatggatc tattgaaaaa acagttttac
    14221 accatttgta aagcaatcga ttttaacgat gaggtgttta aatcaaacag ttctggagaa
    14281 gctcgcaagt ggcaaatcat tagcctagaa gcgaaaacaa atactaaaga gcaatatttt
    14341 agagaaggat tgaaagaatg cgcagaaaca attgctgctt ttcttaagtt tcatgacaaa
    14401 gtagaaattg agcctgaaaa aattattttt actttctcta gatctttgcc aactgattta
    14461 agttatttag ctgaagcttt acctaaactt gcaccatacg tatccaaacg aactattcag
    14521 agccaaattc catttgtaac agatgttgat tatgaaaatg aaatgatgga attggaaagt
    14581 ggaagtgctt atccagatag tgaatacaat tttggcggag gtggcaatag tgacgataga
    14641 aacgaagtat tggaccaaac gtcgagaact ggaggatcaa gcaaggctca aacaagaaaa
    14701 tcagacactt aaaaaattaa ctagtgtatt tcctgaagca ctgaaagaga tacaagcaaa
    14761 actattatca caagctgact tacacaatat cacttatcca gaaatgatgg agttttacag
    14821 tacaagtaat cagaaaaaat atcgtgaata tgtggaaaaa aattataagt cattaaaaat
    14881 gtatgatgca aaatacaaag agtttatcga tgaatttttt ccaccatttg actatgcaaa
    14941 agtcaatcgc ttattacaaa tacgatcaga tgtatttaaa attcttgcag aatatgcgat
    15001 ggatgcagat gtgaatcaat atttttctga tcgcttagag gaaattcttc aaagaacata
    15061 ttcttctaat gctaatgttt ttgttcaact tttaaacgtt gatataccaa attatttacc
    15121 agaaaatgaa attaaacatt atttgaacta tccatggtca ggaaagacat tttcaagaag
    15181 actttgggga aatatttcat cgcttgaaca gaaactctct aatgctattg taaaaagtgt
    15241 tgctagtgga gaaggcgtta tacacgcatt aaacaccatg agattagact cagaaatttg
    15301 tgacatgttt aagttagaag aatcaaagta taacaaagcg atagaaaatc tcgttcgaac
    15361 ggagtatgca aaatttgcac aagatggtat tgaaaaatca tatttagaaa caggtattga
    15421 ggaatacaac gtattgactg caaaagatga gagagtttgc cggatttgtg gaggaaaggc
    15481 aagtaagaat ccctataaac tgaaagatgc tgtcataggt gaaaatcgag cacctttcca
    15541 tagtcgttgt agatgtacgg atgttcctaa tttaccaaaa ttaggaaagg atattgatga
    15601 agaatatgac cgtttatttg gcgatttatt agatgagttt gcacatgatt cttttggaat
    15661 taatttgaaa cggaggaagt agaatgaaag atttttttga agcagtacta acaattaatg
    15721 taaatgctga tattgcagaa gcctacaaaa cagctattga gtctgagaac catcctaatg
    15781 gcttgagaga ccattggaat ggcaattatg cctacgtggt tattggcgat caaactgtta
    15841 attatcaaga taatactcca gttgataaga ataccgttaa tttaacgatt caattattat
    15901 ctcattcatt accaaattta aaagaaacag ttgattggta tgaaaagatg ggatgtattg
    15961 ttgttagaac tgactacaaa gaaggaaagt ctagtaatta ggctttttta ttttgtccga
    16021 aatgacgtta aactagcgca atactgggct tggttgaatg gtggggcgca actattaaaa
    16081 ctcaaagcaa tgcggggcgt gaaaacgaat cgtggggcga aaggagaatg agcatgaaac
    16141 acaaatcatt aatgccaatg aatttgcaat actttgccga aggtgacgat caaaagtttt
    16201 cttttgatga cttcaaatct tttgtggaat ccaatgaaga agctcaaaaa tttattcagt
    16261 cacaatctca aagtgttgcc gacaaacaat tggaagcttg gaaacaaaat aacttagata
    16321 aaattaaaca ggataccatc aaggaatatg aggaatctaa gaaaaacaag tcacctgagc
    16381 aaattcaact ggaaaaacta caagctgaat ttgaagcaga aaaagcgttg cgtgtgacaa
    16441 gtgataataa agcatttgtt gcagaacaaa ttgctggatt agaactagat ggagagttaa
    16501 aagagtctat ttctcaattt atgctaaata atcttgttag ttcggataca gatttcacta
    16561 agaatgctgt tgaaggtttt acaagtgttt tgaatgcaat taatgagaaa catgcagatg
    16621 cattaaaaga actacaaatg aagtctgcat ttggtggaac tcaacaatcg aataaccagg
    16681 ttcagcagaa caatgaaaca ttaacaaatc cagaagaaca attaggacaa attcttcaac
    16741 aatttaacta ggagagtgaa aaattatgaa aaaaacatct ttaaataatt tagagtattt
    16801 ggatatttca ccagcgatta atgctatgca agtaccaaat acaccttttt taagctattt
    16861 acttggtgct ggaaaaacag agccagcaaa ctcgacagaa attaaatggc gtgaatatga
    16921 tatcaacaac gatgattctt ctgaaaaact tgagggcgga gaatatccag atgctgaatc
    16981 aggtcgaact tggtttaaca actatactga aatttttaga aaatcaacct ctgtatctgg
    17041 tacattagat gctattaatg tgaatggagt cggaaatgaa ttaactaatc aagtagcact
    17101 acgtggtatg gaaatgaaaa ttgatttgaa ccgaaaattg attactggtg taaaagctga
    17161 tgaaaatggt tctaaaggtc gtcgaatgaa tgggattttg aacttgatca attcagcaaa
    17221 taaggcagaa acagccactg cgggtgcagt aacaagaaaa gatatcgata ctttatttaa
    17281 attgatgtat gaaaaaggtt atatgggaga aaaactatgc ttgatttctc cagatatgca
    17341 ggagttaatg actgatgagt tagatggaaa atcaacaaaa attgttcagt tcggggaaag
    17401 agtaactttt ggattgcaaa ttggaaatat cgtgtctaat tacggtacag gtattgctct
    17461 actagaacca tcattgccaa aaggaacaat tgccgcaatc gatactaatt atgtgaaact
    17521 acgtccatta cgtgaatgga gagcagaaga acttgcaaaa acaacggatt ctagacgtat
    17581 cggtcttgta ggtgaatact ctcttgaata caatgcttca aactctgggg caattttaaa
    17641 tttaaagtct gaataaaagg gagttagtac tccctttttt gataggagga attacagtgg
    17701 taaaaaaaga tgaaactaaa aaagatgaag tcgtgaaata tagagtaggt aaaactaaaa
    17761 attttgttgg gtttgttcat cctaaaactc gtagatttat cacagcagat tcaaataacg
    17821 aatttatcat ttctatagat gataaagaag caattgcaat tttggaagat gcaattgatg
    17881 ttaatgaaat ttaggaagtg atctgatgga tgaatcgcta aaaacggaaa tcattgagtc
    17941 tacaaaagaa gattttccag atttgagtga agaacgcata actaatttat tagaaataat
    18001 tttgctagaa attgaatcat acaacacttg taaaaatgat atttcatggg aaaagttaaa
    18061 aagcgtgatt agtgaagtgt tgtataaaat aataaaaaat gaatcagaaa aaacagtatc
    18121 ttcagttaga cgtggcgata caacgattag ttatgcttca acaacaaatg atgttagtga
    18181 gttacttcta ggctatggtg atttgatacg aagagttatt ggttgtggag gattggagtt
    18241 tttttaatga atgaagcaga tattttagaa actacttatg aagatagttg tattattgaa
    18301 agactgacgg acattgaaga ttctaataca aatattacta ttcaagatta caaaaaagta
    18361 tatgataatc ctatttcttg cgctctttca caaggtcaaa tcgatggact agcagtcata
    18421 gaagatggag agatggtaaa tgtttcaact gacacatata aattatttgt tcatcctaag
    18481 attaaactaa aaaaaggaga tcgaataaca ataactcaaa aggccagtgg cttaattttt
    18541 tctctatttg ctactaagcc tttttactat cctagtcatt gtgaagtgaa tttgatagga
    18601 agtgagaaaa atgggtaatc tcaaatatga atcaaatgca gaaaagatca ttgagaattt
    18661 taaaaatatg actgtaattg ctcaaaaaga aggaatatcc ttcgttaacg attcaatgaa
    18721 taaagtcgtt agtttagcta aaccattaac tcctgtaaaa tcaggtaatt taaggcgagg
    18781 atatcgtgta gtaaaagcta gaaaactatc aagcggtcga attgttggag aagttataaa
    18841 caatgaacat tattttaaat atgtagaaga aggtcatagg actaaaaatg gtggctttgt
    18901 caaagggaaa ttcatgttaa ctcgtgcaac gaatcttgca aatatgtctt atattcctcg
    18961 aagatttaaa caaatggcaa taaaaatcgt taagaaagga aagtaacatg aaagatgaaa
    19021 tcattgctgc aatcagtagc aaattaaaag aaatctatcc tgatgggaca atatatcttg
    19081 attcagttat gcagtcaact aaagattttt atttcgtttt atcagtaatg gaatctggaa
    19141 ctgaaaatgt aggaattgat attcaaaatg tttctttctt aattgatatt gcattgattg
    19201 ataataaccc taatagaaat ttaattaatg aattagtctc acgttgtggg acttttttta
    19261 atacgattac aatcgatgag caaatattat ttccaaaagc ctatttacct gatgaagcag
    19321 atggtgttca acatattcgt tttacattag aatttccaca atatattgaa tggagtgaaa
    19381 gataaatggg agaaaaaaga agtaaaaccg gaattatttc tgtcgaaaaa cctacttggt
    19441 ttcctttgga agatgagaca gggaattttc cggtatatgg tactgcgttg ccaatgggaa
    19501 cagcagtaag tatcaaacca acagctaatt atgaaacaac acaagattat ggggattcag
    19561 tggttcaaga tcaatttaca gcttttggtg gtgcagaagt tgaactggaa gcaaatggat
    19621 atactcataa agttttatct acaattactg gagggaaaat tgttaaaggt ggtgccttgc
    19681 gttctggaga agatattgcg caagatggtg cgtttgcata tagacgtaaa aaatcgaatg
    19741 ggaaataccg ttatacggtc ttttataaag gacagtttgc tctagattca gatgaaacat
    19801 caacgattga aggaagtaaa gtaagtttta ctcatccaac ttggaagggt tcatttgtag
    19861 atgtaccagg acttggatat atgtattcag ttgatgaaga tgatgaaggc gtggataaag
    19921 caatgatcgg aaattggttt acaaaagtag caattccaat tgaagaagca gaactttcag
    19981 gaggtacaga ataatgtcaa aatatcaaac aacgattaaa ttaacgaaaa aagacgaaga
    20041 aggtaaatat gagcaagtac aatttaaatc tgctgaattt ttaccaggaa cagttgtaga
    20101 agatgctgca ggagttatgg aagaaatgca aacagcgact gataagcaat cagttaaaaa
    20161 agctttaagt cgtgcttatt catttattgc agatacttta tttgagggac aatttactgg
    20221 tgaagattat tgcaaaggaa ttgatgctcg tgagattgcc tcattgacag gaaaattatt
    20281 gaagtctgtt actgcaggtt ttgatgaaac ttatacagag acgaaaaaaa agtaagtgag
    20341 gcgctcaagt caccttcatt taagtattcg attacttacc gagaattaga tataaagacg
    20401 caattactcg aagcaggttg gacgttacca gaaattgaac gtactgactt ggatgagtta
    20461 atgcgtcttt ttgcttttag agatgcggta aaagagcatg aagatgttga gtactatgat
    20521 aatttcactc aattttagga ggtgatactt tgaataacga tgacctaatt ctgaagatga
    20581 tactagatga atctggtttt actgccggta tgaataatgc tgtcaaaaag ttgaactctt
    20641 ttgatgaaac aatcgaaaga aaaagtagaa atagtggcag ttcattaggt agtatttgga
    20701 aaatatttgc tggaagtttt ctagctagcg gagtaactag aatcgtagga gctggctttg
    20761 atctaatcaa gggttccata agtggagcgg ttgatcgagt agatacgatg aataacgctc
    20821 tacgaaattt ccaaaatatg ggatttagta attcagaaat tatgaagaat atcggaaaga
    20881 atgggctttt atctcaaggt attcaaggac ttcctactgc tttgaatgat gcgataagtc
    20941 atgttcaact tcttgcttct tctacaggtg atttaactcg ttcaactcaa atatttaaag
    21001 ctttaaatga tggaattctt ggtttcggtg gttcaactga tcaagttaat gaagctgtta
    21061 ttcaattatc tcaaagtttc tcaaacggaa aagtagacgc acaaacttgg aactcaatga
    21121 tcaacgctca acttggacct actctttctg ctattgctaa aaagatggga attacaatgg
    21181 gagatctgaa agaaggttta tctcaaggta agatttctgt tgaagaattc caaaatcaat
    21241 taatagaaat ggataccaaa ggtggcggag gacttaaatc attaagtcaa atcgctaaag
    21301 attctactaa gggaattaaa acctctatac aaaatgcaaa aacagctgtt acacgtggtg
    21361 ttggtgaggt aatagaagga ttaaataaag cattagtaga ttcagattta ggtggattta
    21421 agggaattat tgataaagtg ggcagttcaa tggaatcgtt cttaaaagta attgctgcaa
    21481 atattcctac agcagtatca tttttaagta atttatttaa cgaaattcaa aaatttggtt
    21541 ctgctttgaa attcatgatg ccctttcttg ttcctgcagc taccgctttt ggagcgttca
    21601 tgtttcaact taaaggtata ccagcaataa taaaaagctt caataatttt aagaatgcca
    21661 taattggcgt tggaaattca ttaaagatta tgggggcaat agctgctgct aatccatttg
    21721 ttttaattat tggtgcagtc gttggtgcaa tagctgtttt cggttacttt atggcaacca
    21781 atgaagagct tagaaacaag gttatatcta tttggaatga tgtgaaagat tccgtaattg
    21841 gtgtattaaa aaatataaag gactggggaa ttgatacttg gaattctgct aaagaaatgg
    21901 catcaaatgc agttgagggt gtcaaagatg cttggtcagg aacaaaggaa tggttttcta
    21961 acacctggaa aggtattaag gatggggcca caggtttatt tgataaaaca gtagagactt
    22021 caagaaatgc agtcgatagt gtaaaaaatg catggtctaa tatgaagaaa tggttttctg
    22081 atacttggca aagtataaaa gattcagcca gagaaaaatg gaatggaatc aaaggttcta
    22141 ttatgtcagt tgctggtcca ttgattacag gtataaagaa tgcattttta catgttactt
    22201 tttatctaga gaccttatgg aataatttag ttgagattgg taaaaacgtc tttgagattt
    22261 taaaaaatgt tatacttgcg ccggttttgt ttattacctc catgatttct ggtggatggg
    22321 aagaaacaaa aaataatatg attgctgttt ggaataatat taaagaaagt gccataaata
    22381 tttgggaatc tataaagaat atatttgtaa gttattttac aaatatttat tttgctgcac
    22441 tcaatatttg gacagggttc aagcttacat tgataaat
//
LOCUS       DAZKLA010000037        22301 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000037 DAZKLA010000000
VERSION     DAZKLA010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.037"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            27..845
                     /gene="purR"
                     /locus_tag="ACG85N_001212"
     CDS             27..845
                     /gene="purR"
                     /locus_tag="ACG85N_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFQ0223996.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1348..2721
                     /gene="glmU"
                     /locus_tag="ACG85N_001213"
     CDS             1348..2721
                     /gene="glmU"
                     /locus_tag="ACG85N_001213"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFQ0223997.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2815..3786
                     /locus_tag="ACG85N_001214"
     CDS             2815..3786
                     /locus_tag="ACG85N_001214"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0223998.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3792..3894
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3960..4529
                     /gene="thiT"
                     /locus_tag="ACG85N_001215"
     CDS             3960..4529
                     /gene="thiT"
                     /locus_tag="ACG85N_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFQ0223999.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4610..5383)
                     /locus_tag="ACG85N_001216"
     CDS             complement(4610..5383)
                     /locus_tag="ACG85N_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224000.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5650..7350
                     /locus_tag="ACG85N_001217"
     CDS             5650..7350
                     /locus_tag="ACG85N_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFQ0224001.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7320..7538
                     /locus_tag="ACG85N_001218"
     CDS             7320..7538
                     /locus_tag="ACG85N_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFQ0224002.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7558..8448
                     /locus_tag="ACG85N_001219"
     CDS             7558..8448
                     /locus_tag="ACG85N_001219"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFQ0224003.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8466..8750
                     /locus_tag="ACG85N_001220"
     CDS             8466..8750
                     /locus_tag="ACG85N_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224004.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            9237..9851
                     /gene="gmk"
                     /locus_tag="ACG85N_001221"
     CDS             9237..9851
                     /gene="gmk"
                     /locus_tag="ACG85N_001221"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0224005.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            9856..10161
                     /gene="rpoZ"
                     /locus_tag="ACG85N_001222"
     CDS             9856..10161
                     /gene="rpoZ"
                     /locus_tag="ACG85N_001222"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0224006.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            10414..12822
                     /gene="priA"
                     /locus_tag="ACG85N_001223"
     CDS             10414..12822
                     /gene="priA"
                     /locus_tag="ACG85N_001223"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFQ0224007.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            12837..13328
                     /gene="def"
                     /locus_tag="ACG85N_001224"
     CDS             12837..13328
                     /gene="def"
                     /locus_tag="ACG85N_001224"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0224008.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            13321..14259
                     /gene="fmt"
                     /locus_tag="ACG85N_001225"
     CDS             13321..14259
                     /gene="fmt"
                     /locus_tag="ACG85N_001225"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFQ0224009.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            14259..15617
                     /gene="rsmB"
                     /locus_tag="ACG85N_001226"
     CDS             14259..15617
                     /gene="rsmB"
                     /locus_tag="ACG85N_001226"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFQ0224010.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            15630..16370
                     /locus_tag="ACG85N_001227"
     CDS             15630..16370
                     /locus_tag="ACG85N_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFQ0224011.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            16367..18436
                     /gene="pknB"
                     /locus_tag="ACG85N_001228"
     CDS             16367..18436
                     /gene="pknB"
                     /locus_tag="ACG85N_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFQ0224012.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            18601..19500
                     /gene="rsgA"
                     /locus_tag="ACG85N_001229"
     CDS             18601..19500
                     /gene="rsgA"
                     /locus_tag="ACG85N_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFQ0224013.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            19513..20163
                     /gene="rpe"
                     /locus_tag="ACG85N_001230"
     CDS             19513..20163
                     /gene="rpe"
                     /locus_tag="ACG85N_001230"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFQ0224014.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            20164..20802
                     /locus_tag="ACG85N_001231"
     CDS             20164..20802
                     /locus_tag="ACG85N_001231"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFQ0224015.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            20957..21811
                     /gene="mreC"
                     /locus_tag="ACG85N_001232"
     CDS             20957..21811
                     /gene="mreC"
                     /locus_tag="ACG85N_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFQ0224016.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            21815..>22301
                     /gene="mreD"
                     /locus_tag="ACG85N_001233"
     CDS             21815..>22301
                     /gene="mreD"
                     /locus_tag="ACG85N_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFQ0224017.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PF"
BASE COUNT         7426 a         4005 c         4841 g         6029 t
ORIGIN      
        1 aaatttctta aaaggagttg gaataggtga aagtacgtcg cagtgaacgt ctagttgaca
       61 tgacacagta tttattgaat cacccgcatg aattgatttc cttaacaagc tttgccgaac
      121 gttatgaatc tgctaaatct tctatcagtg aagatttggg aatcatcaag aaaacattta
      181 aagaacgagg gtatggaacc ttggaaacaa tcccgggtgc tgcaggtggt gtattgttca
      241 tccctgaaat ttcatatgaa gaggcgaaaa aatatattga atctctagca gaacgtctat
      301 cagaacaaga ccgtctgctt ccaggaggat atgtttatct ctctgatttg ttaggagaac
      361 ctgaactgct gcgtcaagtt ggaagaatca ttgcttctaa ataccttggt gaaaaaatcg
      421 atgctgtaat gactgtagcc acaaaaggcg tacctattgc ccaagctgtg tcctattatc
      481 tgaatgcgcc attcgttatt gtacgccggg actcaaaaat aacagaagga tcaacagtaa
      541 gtgtcaacta tgtgtctgga tcttctgaaa gaattgaaaa gatggaactg tctaagagaa
      601 gtttgaaacg tggttcacgt gtccttgtag tggatgattt catgaaaggc ggcggaacag
      661 tcaacggaat gaagagcttg attgaagaat tcgaatcaga acttgttggc atcactgtat
      721 ttgccgaatc aaaattcaac ggtcatcgcg ccatcgatga ttatacgtca ctgctttatg
      781 tcaaagatgt agatactcaa acgaaaaata ttacggttgt gccaggaaat tatttttcag
      841 aataagaggt tgtgaaaaag ccgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
      901 gctttccaca ttttttcata aatttggctt aataccgaga aactggcttt tgaacaccgt
      961 ttatctagag gttgtgaaat cagcgtcctg tcctgagtat tagctcaata aacggaaaaa
     1021 ttgctcctca tattttgccg tttattgagc taataccgaa ggtcagctga ttgaaaaacg
     1081 tttaatgaag aggttgtaag cctaccgttt aactctaagt cacaagaaac gatttcaaaa
     1141 aacagctttt catgttttat gagtagtgtg acttgtgacc gaggagttgg caggagagca
     1201 ccggttacca aatagatagg cccgttatgg tctatctatt tttttatcca gaaaacgctg
     1261 ggataaaaga aaatcacttg ttttgttgaa aaaatgggta tactgggata aactgaaatc
     1321 agcaaactat atttaggagt gacgcagttg gacgcacgtt atgcaattat tttggcggca
     1381 gggaaaggta cccgcatgaa gtcaaaatta tataaagttt tacatcccgt ttctggtaaa
     1441 cctatggtag aacatatcat taatcgggtc agtgaaacga aaccagatga agtgattacg
     1501 atcgttggac acggagcaga acaagttaaa gcacaactag gagaacgcag taaatatgcg
     1561 ttacaagcag aacaattggg aacaggacat gctgttttgc aagctgcttc tttcttggaa
     1621 ggaaaaaaag gaacaacgtt agttatcagt ggcgatacac cattattgac tactgaaacg
     1681 ctgaacaatc tttttgaata tcaccaagga aaaaatgcaa gtgcaacgat cttgacggca
     1741 caagcagaaa acccaacagg atatggacga atcatccgtg accatattgg gatcgtagaa
     1801 aaaatcgtgg aacaaaaaga tgcaacacca gaagaagctc ttgtacaaga aatcaataca
     1861 ggaacttact gttttgacaa cgaagctttg ttcgatgcgt taagcaaagt tggaacaaac
     1921 aatgctcaag gtgaatacta tctaacagat atcatcgaaa tcttaaaaga agaaggccat
     1981 acagttgctg cttatcaaac ggatgatttt gaagaatcaa tgggagtcaa tgatcggatc
     2041 gcgttagcta aagcaaatga gatcatgaga aagcgaatta accagatgca tatggtcaat
     2101 ggcgtaagct ttgtcgattc agcaacgaca tacatcgatg caggcgtaga aatcggtcca
     2161 gacacattga tcgaagcagg tgtacaaatc caaggaaata cagtcattgg ttctgattgt
     2221 gtcatcgggt cacattcaaa aatcgtagac agtaggattg aggatcacgt cgtcattgaa
     2281 aactcagtca ttgaaagcag ccatgtcaag aaacatgcag atgtgggacc ttatgcacat
     2341 ttacgaccaa aagctgaaat tggcgaaaac gtccatatcg gaaactttgt ggaagtgaaa
     2401 aatgctcaaa tcggcaaagg aactaaagtc ggtcacttga cttatgttgg tgatgctacg
     2461 ctgggagaag aaatcaatgt cggatgtggt gttgtcttcg tcaactatga tggaaagaac
     2521 aaacatcata cgactgtcgg cgaccacagc tttattggtt ctagcacgaa tatcatcgga
     2581 cctgtcgagg tagctaaaaa cagttcgatt gctgccgggt caacaattac agacaatatt
     2641 ccagaatatg cgctggcaat tgcgagagca agacaagtca ataaagaagg atatgcaaaa
     2701 aaactccctt atctaaacta agacttttgc aaaatcactt gatttattta aaaaatatga
     2761 ctactattta ttaggtagaa ttctagagtt gtgaaaaaga aagtggaggt ccccatgtca
     2821 aaacattact ttgacccaag attaaaaatt tttgcgttaa attccaatcg cccattagca
     2881 gagaaaattg cagcagcagt cggcgtcgag ttaggaaaat catctgttac tcaatttagt
     2941 gatggagaaa ttcaagtaaa cattgaagaa agtattcgtg gatcgcatgt ctatgttatt
     3001 caatcaacga gcagcccagt caatgataac ttgatggagt tgctgatcat gattgacgca
     3061 ctgaaacgag caagtgcaaa aacaatcaac gtagtcatgc cttattatgg ctatgcgcgt
     3121 caagaccgta aagcacgtgc acgggaacca atcacagcga aattagtagc aaacatgatt
     3181 caaaaagctg gtgcaacaag aatgctgact ttggaccttc atgcagtcca aatccaaggt
     3241 ttctttgata ttccagtcga tcatttgatg ggtgcgccac ttattgccaa ttatttctta
     3301 gaaaaaggaa tcaaaggcga tgatgttgtc gttgtttcac cagaccatgg tggtgtcaca
     3361 cgggcacgta aattagcgga atttttgaaa tctccgattg cgatcataga caaacgccgt
     3421 ccaaaagcaa atgttgcaga agtaatgaat atcattggac aagtcaaagg caaaacttgt
     3481 gtactgatcg atgatatgat cgatacagca ggtacgatca cattggctgc caatgctttg
     3541 aaagaagctg gtgcaactag tgtgtatgct tcatgtacac atccagtatt atccggccct
     3601 gctttacaaa gaatagaaga ttcagctatc gagcgtctag ttgttacaga ctcgatctat
     3661 cttccagaag atcgtaaaat cgacaaaatc gatgaagtaa gtgttggcgg actaatggga
     3721 gatgcaatca aacgtatcca tgaaaacaaa ccagttagtc cattgtttga aacaaaaaat
     3781 aaataataaa taaactcaca aaggggagtc caattgggct gagattgaat ctatttctaa
     3841 acccttcgta cctgtatcgg ttatgcgagc gtaggaattg tgaataaaca gctgtttgac
     3901 tattttcgaa agctgcttcc tcctttgtga cttttttatt caaaggagga agttttttta
     3961 tgggaaaaaa tagagtttgg gtagaaggaa cgattgttgc agcgttagcg atggtgttgt
     4021 cgttgattcc gatccagatc ggcagcagtt tttctatttc attagggcag attccattga
     4081 ctctttttgc gttaagaaga gggacaaagg cagggatgct ggcaggtttc atttggggca
     4141 tcctacattt tccgttggga caagtttatt atttaagtgt cgttcaagtg ttgattgagt
     4201 acccgattgc ctatactttt gcaggcgcag cagggctcat ggcttcaaat gtccaaaaag
     4261 gctatgtaga cgataatacg aaacaaatta gaaaaagcat cgtgtttggt gcattgattg
     4321 gcgcactttc cagatatttt tggcatttta ttgcaggagt ggttttctgg ggagcgtatg
     4381 cactatgggg gatgaatcct tggctctttt ctttcgtaat gaatggtgcc agcgggttga
     4441 gtacagctat tgtaacgatc atcgttttat tagggatcca acaaacgact ccttcgattt
     4501 ttgtgccaga taaacgacag gagctgtaaa taaaaaagaa gcaagaaact tgagacagaa
     4561 tcatgtccta gtttcttgtt tcctttttat tttttgataa gaaatagatc tacttcaatg
     4621 gcagataaga aagatcggca tcttctacca attgccagtg tcgatcggtg acagagtaga
     4681 ttaaagtagc gatgatttcc tcttctcgat accgggtgcc ttttaatttc caaatataat
     4741 cttcaatcac atagattggg atacgatatt taccatcgat caaaccaact tgatcaaaag
     4801 aaaaaatgat cccttcctgc atcaaattac gcatttcttt caataattcc acactgattt
     4861 tttcaacagg tgtttctttt ttgaaaaaag atttttttac attgatctta tctgacaaga
     4921 tcgaacttaa tttactagca aaaatcctat aaaattcatc taaatagcga taataaatcc
     4981 gattcagctg atcattaaga tcgaagtata caaaattatt ctgtagttta taaaaaaacg
     5041 gcgaatgaag atgtgttttc atatgaccaa aatataataa ttctgaaatt tctaaaggtg
     5101 tcaattcttt cagcatcgtt aaatcagtaa agtcgatcca cttcaattca tcgaatccac
     5161 gcctgttacg agaagaggaa aagaaacgct gaatattttc tgtaccacgg attactttca
     5221 gccctgtatg ggattcatat tctccagcag aagcagatgg atcaagcaag agcaagtttt
     5281 gaggaaaatg cacgatactg tttgcaaagt cagtagcagt cagccctttt gttaaaacag
     5341 cattacttgt ggtatcaata tgtacataaa ctgactcagt catcatgttc ctcctcttat
     5401 ttcctcttaa tctcttttat tttacaacat ttgcattaaa aaagcttgtt tcttttattt
     5461 taaaattcca ttacaaaatc acgttttcat tgaaaacact ctcattttca attaattttt
     5521 gtgatttttg tattttttta tttaaaggag gttttatgaa ttgttttctt atggaatcta
     5581 caaagtcgaa tacgagactc atgacctttt tatgacgtac tggtatactt gaagaaaaag
     5641 gagagcgcga tggagataaa aaaagtttac ttttcacaga aaatgtcgct gtattcgcct
     5701 atccagcaag caaaacaacc ccttccattt tttcaaagtc atcaagaaag tagagacgct
     5761 ttttggcgtt ctgcaaaaaa gcaatgtgct tttaaagcag tatcgaaaaa atataccgtc
     5821 tattttacta ttgctaaaag tagaggagat gttattttta ataatctgtt gattgaaggt
     5881 gaaacttttg aatggaaaaa attttttcgg tatatggaag cttgcgctcg tttttttatt
     5941 aaagaaaact gttgtttttt atttcaatct cctctatcag aagaatggtt gcgtattttt
     6001 cataaaaacg gctatcaagg aaccacacaa ttaaataaaa agctggtcta tcatacagca
     6061 cttgttttag gtggaggagg agcgcacggt gcttatcaga ttggtgtgtg gcaggcgctg
     6121 aaggaacatg atatcaactt tgagattatc acaggtacct cggtaggtgc gttgaacggc
     6181 gcattgattt tacaaggaga tatgaaaaaa gctgtgaatt tatggaaaaa gctgtcgaca
     6241 agacaagtgc ttgcgttacc agaaatggct gcagttgaag accttcgtga acgcttctat
     6301 cgagaaagac gccaaatgac gaaaacagca ctaattgaag gcggggtttc ttctgctcca
     6361 ttggaaaaga tggtcaaaga caatttggat ttgtccttac tcaaacataa tcccaaaata
     6421 cgtttatata cggtgtctac taaactgcca gaactagcgg aagtagtgac tgatatccaa
     6481 cagacaaaaa cagaagaaat accagattgg atactcgcct ctgcttcatt ttatcccgct
     6541 atggcttatc gaaagatcgg aaaaaataaa tatgcagacg gtgggtatcg caacaaaata
     6601 cctattgata ttgcgattaa caaaggagca acagaggcat ttgtcgtaga tgtgcaaggg
     6661 ccaggcccag cgaaaaaaat tcgtatgcca gacattttta tccattggaa atgtcaaaca
     6721 ttgtggacac taggcagctt tctgcttttt gatagccaaa ggaaccaatt aaatttacaa
     6781 ttaggctatc tggaaatgaa aaaacgatta ggctattact atgggaactg gtatacattt
     6841 cattctgtta aacctgcaag aacatactgg cgaggatttt tgtcttactt aacaaaagaa
     6901 attcagttag aattatcttt tttcaaaagc ataaaatttt ggcaaaaact gcgtaattta
     6961 tataagaaca gggtcgtacc agagacatgc ggattggcga tgctagaact tttagcaaaa
     7021 aaacattttt tactgcccaa tgaaatctat caagtagaca tgatgattca aatgatctgc
     7081 caacaagata taaaaagtat gccggatgat ttattggcac aaattggtca attaagtact
     7141 gaagaatggc gaaggtatcg aaaataccag caaaaaatac aaaatgagcg cacgaaaact
     7201 gtttattttg attatctgct acaaggaaaa cgcaaggatc gtttgcagca tgaattgctt
     7261 aagcaaccag ttgacacatt actgatccta tatttatatt atctcaagga ggaacaacca
     7321 tggcacaaga attttcatat gaaatcgtag aagaaatcgc tatattgtca gaaaacaaca
     7381 aggggtggcg taaagaactg aacctagtca gctggaatgg tcgcccgcca aaattcgatc
     7441 ttcgagattg ggcgccagac catgaaaaaa tggggaaagg cctgacattg acgaacgaag
     7501 agtttgaaca actgcaaaaa gcaatagaaa atatgtaatg aggaaggacc ctgtcgaatg
     7561 aaaagtatga ctggttttgg caaagccatc cgcgaaacaa aagcgtatca gttagatatt
     7621 gaaataaaaa gtgtcaacca gcgttttcta gatgttcaaa tacgaagtcc taaaatcctg
     7681 aactttgttg aaaacgattt tcgtcagctt attaaacagc agttaagccg cggtcgaatc
     7741 gaagtatttg tgaatctagc ttatattggc aaaaacgaaa aacagatcgt catcaactgg
     7801 gaattgattg attcacttat cactcaattg acagacggaa ttcaacaacg atacggtagt
     7861 caaaatcaag tgtcaattag tcggatggcg gaacgcttcg tgctggacga aaacttcgta
     7921 acggtagaag aaaaaccgga agagagcatg gaagaattgg cacagttagc tctagataca
     7981 ttgaaaagcg cacttgattc tattgaggcg agtcgagaaa aagaaggtca agctttagct
     8041 ttagtgttga aaaagaatac tgaagaattc aaagaagtac taaaaaaact cgatagtttt
     8101 gtagaaatct atgaaaagga atttcaaata aggtatcaaa aaaagctgga ggaatttctt
     8161 ggagcgaaag tcgatgagca acggttgttg acggagctag ccatcttgtt ggaacgcgga
     8221 gatattcacg aggaactgga tcgtctggcg atccatgtac aaaaactaga tgaacttatc
     8281 gaagcacaat gtcctgtagg tcgggaatta gatttcctga tccaagaaat gaatcgggaa
     8341 atcaacacga tcggttccaa atcaaatgca atcgaaatta aaaatcaagt cgtccagtct
     8401 aagacgatct tagaaaaaat cagagaacaa gtacaaaata ttgaatgacg aaagaggtag
     8461 agataatgaa atattatgac gtaacgttcc acgaattaag tggaaaaact gtggttaaac
     8521 gggatatccc ttctgaaaaa aacggctttg acgtatggaa agatgcctgt gcagactata
     8581 atgagaacga actgtttatt ttgatcaacg acggtgctta cgtgacgatg aatcggaaat
     8641 ttattgttcg aattgacaca gaagaggtag aagatccgac agaaaaagca cgttctcgaa
     8701 aagatgagat catgggtgta gtgaatactt tgtccaatat gggcttttag caaggatgct
     8761 gaacaaagaa atacaataaa taaacagaat tggatttctt cgcttagctt ctttgctcta
     8821 ggaatacatt taaaggttgt gacaaaattc gtgtcacaat ctttttttat tttgaataag
     8881 tgatgttcaa aaaccatttt ctcggtatta agccaaattt atgcaaaaat gtgagaaact
     8941 attttcacaa aatttttctt catactcgaa actaatcggc ttcttcacga cctcttatac
     9001 acggcgttca aaagcttgca tctgcgataa taaccccaac caaacgaaaa acatgagaag
     9061 ctgtttatgt ttggctgttc ttattacttg atgcaggaca gcttttttac gacctcttgg
     9121 tacagggtag cttttttacc aatttctagc tataattaaa gataaccttg aaagttgcag
     9181 aaaaaaagtg caaaataaac gataagtaca aaaagactag ttgaaaggac gtcgtcatgt
     9241 cagagcgtgg attattaatc gtgttgtctg gtccatccgg tgtaggaaaa ggcacagtac
     9301 gaaaagcaat ttttgagagt gaggataatg attttcagta ttcgatctca atgactaccc
     9361 gtaaaatgcg tgaaggggag gtcgaaggcg ttgattattt cttccgaagc aaagaagagt
     9421 ttgaatcaat gatcgaagct ggagagatgc tggaatacgc agaatatgtc gggaattatt
     9481 atggaactcc tttgtcatat gtcaacaaga cattggatga aggaaaagat gttttcttgg
     9541 agattgaagt ccaaggagca aaacaagtca aagaaaaagt gcccgatggt gtattcatct
     9601 ttttgactcc gccagattta gcggaattaa aatcacggat cgtaggccga ggaacagatg
     9661 cagatgaagt aatcgaaaaa cgaatgaaag ttgcaaaaga agaaatcgaa atgatggctc
     9721 tttacgacta tgctgttgta aatgatgaag tgccgctagc agttgaacga atcaaaaaca
     9781 ttattgccag tgaacatttt cgagtagatc gcgtaatcgg taaatatatt aaaatgttaa
     9841 aggagatgta gtcttatgat gttaaaacct tcaattgact cactattaga tcgtgtaaat
     9901 tcaaaatatt ctctagtgat tttagcaagt aaacgcgcgc atgaattgga tgcaagtgca
     9961 cagcctactc tagattcttt tgattcagta aaaagcgtcg gtcaagcttt agaagaaatc
    10021 gatgcaggaa atgtagtcaa tgatccacat cctgaattaa aacgtgaacg tttgaagatg
    10081 gaagaagagg aacgtcaagc acaaaaagag cgtgaacaac acgaattgga atcacgtatc
    10141 cgtgaagagc aaaaaatgta ataaagaaaa atagagcgaa catctaaaaa gaaaagcaaa
    10201 cccagctgtt tccttttcaa acattcgcca cttattgtct ggtagaaaag gctgtgtctg
    10261 aactttttga cacagttctt ttttttctta tctgctgtta attttttgtt aggatttggc
    10321 agaagataag acaaatccat cgatctctag taaaataggc aaggaacttt ttgtcgcttg
    10381 ataagaaaag acaaaaaaag gaggaaacga accatgacat taacagctga agtcattgtg
    10441 gacgtaccta ctatgcagac cgatcagcca ttcacttatt tggttccctc tgaaatggaa
    10501 acagctttgc aagtcgggat gcgagtagaa gtcccttttg gtaatggcaa tcgtcatgtc
    10561 caaggatttg ttatggctat ccaaaaatca gaagaatcag ctaatccatc tttgaaggca
    10621 gttattcgtt tgctagacct tgctccggtg gtcaatgaag aattactttc attagctgat
    10681 tatatgaaaa aaattactta cgcattcaaa atcacttgtc tacaaacgat gcttccgagt
    10741 gtgatgaaag cagaatatga taaattgatc tatcctttgg cagacacacc agaagttcag
    10801 gtattgtttg atcagcgaga agtgatttcc tggaaggaag cagaagaaga aggcagcctt
    10861 tctcaattga ttcgctggcg ccaagagcaa ttagtagata tcaaatatga agtccataca
    10921 cgaaataaag taaaaacgat tcgtttggtc cgttctttgt taactgaaaa gcaaatcgaa
    10981 gaagaatggg caaaattacg gcaaaatgcc aagaaacaaa aagaacttct tttgtgttta
    11041 tctgaactga gccaagaaga accaattgct tatttcaaaa ataaaggaat cagtactgct
    11101 gtcttgaatc aaggaaaaga aaagggctgg ctggagttta tagaatcaga acgttatcgc
    11161 gacccttata aagaccgagt atttgaccaa acgacggcat tggaactgaa tgcagaacaa
    11221 aaaaatgctg tcgaacaaat catcacagca ggtcagcagc aaaaagatca ggtctttttg
    11281 ctggagggaa tcactggcag cgggaaaaca gaagtatatc ttcaggcaat cgctgatgtt
    11341 ttgtcagaaa ataaaacggc tattatgtta gtgcctgaaa tcgcattgac tccgcaaatg
    11401 gtcgagcgat tcaaaggacg cttcggagaa tcagttgcgg tcttacatag cggactttcc
    11461 caaggtgaaa aatatgatga atggcgcaag attgagcggg aagaagcgca agtagtagta
    11521 ggcgcacgat ctgctatttt tgccccgctg aaaaatatcg ggctgatcat tatcgatgaa
    11581 gaacatgaat caagttacaa acaagatgag acaccgcgtt atcatgcgag agatctagca
    11641 atttggcgca gtaaatatca tcattgccca atagttctag gaagcgcgac gccctcttta
    11701 gaatcaagag cacgagctca aaaaggcgtt tatcagttat tgcagttaaa ccaccgagcc
    11761 aaagcagctg cacagcttcc agctattgag atcgtagata tgcgtgaaga gtttcagaat
    11821 catcggacct ctactttttc tgctaatttg caggaaaaaa tacaaaatcg attagacaaa
    11881 aaagaacaaa ctgtcctact gctcaatcgt cgaggatatt cgtcttttgt gatgtgtagg
    11941 gactgtggat ttgttcttcc atgtcctaat tgcgatatct ctcttacact gcatatggat
    12001 acacgttcaa tgcgctgcca ttattgcgga catgaagaac ctattcccaa tcgctgtcct
    12061 aattgtggcg gcaataaaat ccgctactat ggaacaggta ctcaaaaagt agaagaggaa
    12121 ctccgagaac tttatccgca agctaggatt ttgcgtatgg atgtagatac cactcgaaga
    12181 aaaggggcac acgaacagat tttacaaaaa ttcggtgcaa aagaagcaga tatattatta
    12241 gggacacaaa tgattgctaa aggtttggac tttccagaag tgacattagt tggggtgttg
    12301 aatgctgata cctccctgaa tttaccggat tttcgttcta gtgagcatac attccaattg
    12361 cttacccaag tttcaggacg agcagggcga gcggaaaaag caggcgaagt cgtgatccaa
    12421 acattcaatc ctcaacatta cgccatagaa ttggctaaaa aacaaaatta tgagcagttt
    12481 tatcagcagg aaatgcatgt acggcatcgt ggaggctatc ctccttatta ctttactgtg
    12541 aaaataaccg ccagccatcc tgaagaacaa gtagcggcaa agaaaatctt tcagattgcc
    12601 aatcagctaa aagaagtgtt aagccctcaa agcttgctat tagggccaac gcccagcatg
    12661 attttgagag tgaaaaaccg atattattat caactaatca tcaaatataa acatgaaccg
    12721 aatcttcctc atgttttgga tgaaattttg aatggaagcc agaaagaaca gcgacaaggt
    12781 ctttttgtgg caattgacaa cgaaccatta aactttattt aagaaagggc caaattatgc
    12841 gctatcctat attgatccac cctaatgata aattaaaaag aactgctcag ccaatcgatg
    12901 taatcacgga cgagacaatc gctttattag acaatttata tgagacaatg attgcgaatg
    12961 acggaatagg gatcgctgct cctcaagtag gacaaaacaa gagaattgct gtgatcgaag
    13021 tagatgaagg cgagaaattt gaactgatta acccagaaat catcgaagca aaaggtgaaa
    13081 gccttgacgt agaaggttgt ctcagtattc cgcatgtcta tggaacggtc aagcgagcag
    13141 atgaagtgac cgtgcgttat tatgatcgag acggagaaga gatagaagtg acggcttttg
    13201 gctatttagc ccgtgccttc cagcatgaaa tcgatcactt agacggtatc ttgttcattg
    13261 agaaaatgat acagcagatc ccggaagaag aacttgaaga atatatggag gaacatcttg
    13321 atgactaagc tagtatttat gggcacacct gctttttctg tgccgattct tgaagggctg
    13381 atagaatcag gttacgagat ccaggcagtg gtaacacagc ccgaccgtcc agttgggcgg
    13441 aaacgagtaa ttacgccgac accggtaaaa aaagcggctc tgaagcacgg tatccgtgtg
    13501 ctgcagccag agaaaatctc cggttcgcca gaaatggaag aaatcattga attggtgcct
    13561 gatgtgatcg tcactgcggc atttggccaa ttcttgcctg aaaagttatt gcaagtacca
    13621 aaattagggg caatcaatgt ccatgcttct ttacttccta aatatcgggg aggtgcgcct
    13681 gttcattatg ccatcatgaa tggggagaaa gaaactggtg taacgatcat ggaaatgatc
    13741 aaaaaaatgg atgcaggcgg aatctatgcg caagaaagca taccaatcac taaacaagat
    13801 gatgtaggga cgatgtttga aaagcttagc cttttaggaa gaaagctatt gctagaaaca
    13861 ttgcccaata ttttggatgg acaaaaacca gtacctcaag acgagtcaga agcaactttt
    13921 tcgccaaaca tcacacggga acaagaagca atcgattggc ataaaactgc agaagaaatt
    13981 gacaaccaag tacgcggaat gcgtccttgg ccaattgcat tcacgaccta tgagggcata
    14041 cgctggaaat tattatcggt tgaagtattg gcagaaacta cagacaaagc acctggtacg
    14101 atcatccgga aagacaaaaa aaatctatgg attgcttgcg gtaaacagac cgtcttagcc
    14161 atcaatgaat tacagccagc tggtaaagga aaacaggccg tacatgattt ccttaacgga
    14221 agcggtcagc aagttcagat tggtcagcag gtgaaataat gaaaaaaatc cctaaaaaaa
    14281 tgaaaaaatc agtacgatat atggcattac tgacattaga gcgagtgaac aagggtggcg
    14341 cctactcgaa tcttttgttg aatgagatga tcaaacaaag cgagctaaat caaaaagatg
    14401 tgggtttatt tacagaattg atctatggta cgattagcca tcagcgacta ttggaatttt
    14461 acgttgcacc attgatcgct aaagcaaaaa aagtggatga ctgggtcaag atgcttcttt
    14521 atctttctgt ataccaatta gaatttttag ataaagtccc tgcacatgct attttaaacg
    14581 aggcggtcga aatcgcaaaa gtaaaaggaa atcctgggac agggaaattt gtaaatggtg
    14641 tcttgaggaa ttatcagcgt caaggagcac cagatctgca aggcattgct gatcctatcg
    14701 aaagactttc tgtcaaaatc agcctgcctc tatggatgac gaaacgactg gtggatcaaa
    14761 tcggatacga agaaactgaa aagctcggtt tatcgctata tcagcctagc catgctagtg
    14821 cccgagtaga tacacgccga ctttccagag atcaggccat cactgttttg caagaagaag
    14881 agatcacagc tgaaaaaagc caagtcagtc catacggtat agtagcgaaa aaagggcatt
    14941 tagcttctag ctcattgttc cacgatggcg tcatgacgat ccaagatgaa agttcgatgc
    15001 ttgtagcgcc gtctatgcaa atcgagcctg aacatcatgt cttagatgcc tgtgcagcac
    15061 ccggaggtaa gacgacgcat attgcttctt ttttagatcc gtcatgcggt ggagaagtgg
    15121 tcgctttaga tgtacacgaa cacaaagtca aactcattga agataatgca gcacgtctag
    15181 gtgtctcttc tgctgtctac gctcaaaaga tggatgctag agaagtcagt aaacagttcg
    15241 aagcagagca gtttgatcgg attttagtag acgcgccttg ttcaggtctt gggctgcttc
    15301 ggcgaaaacc agatattcgt tataaaaaac agccggatga attagcgaat ttaccaaaaa
    15361 tccaattgga aattttagaa agctgcgcac caactttaaa gtcttccggt attctgactt
    15421 atagcacttg tacgatctta aaagaagaaa accaagacgt tgtttctgct ttcttggaaa
    15481 gacaccctga ttttgagagg attgatgttt tgaccaatcc tatgctacaa tcatcaatcg
    15541 aagagaaaat gttgactttg tatccgcatc agttttacac agatggattt ttcatttgtt
    15601 gtttgcgcaa aaagtaatga ggtgaaaaaa tgcagattga atatcaatca gatgtaggaa
    15661 gacgacgtaa cacaaatcaa gactatgcca gtgtatttac cagccgttca ggtctcaagc
    15721 ttgctgtatt agcagacggg atgggtggtc atcgtgccgg agacattgcc agtcagatgg
    15781 cggtaacgaa tctaggcgaa gcttgggaaa aacaagaagc tgttgacgat gaaaaaatcg
    15841 cccaatggtt cattcaatcg attcaagaag aaaatatgct gatttatcag cgtggacaag
    15901 aacagccgga atataacggt atgggaacaa caatcgtagc ggccgctctg tcagatgact
    15961 ggttcacgat tgccaatgtt ggggatagca gagcttattt gattcgtgaa gataaaatca
    16021 tgcaattgac ggaggatcat tctttagtca atgaactagt caaatcagga gaaatcagcg
    16081 aagaaatggc agtgaaccat ccaaggaaaa atattttaac acgttctgtc ggcatgcctg
    16141 gaacagtcga agtagatgtt tctactcatc tttggcaact aaaagatcga ctgctgttat
    16201 gttcagatgg cttgacgaac atgctgtctg aagaagaaat aggaacgatc atcaatcaag
    16261 aaggcccatt gtctcataaa gtaactgagc tgattgatcg tgccaatgaa gcaggcggag
    16321 cggataatat caccgttctt cttatcgaat ataaggagga cagtgtatga tcgagttggg
    16381 caaaaaatta aatggccgtt atcaaatcac tggaaatatc ggtagcggtg gtatggcaaa
    16441 tgtatttttg gcgcacgatt taatcttgga tcgtgatgta gcagttaaag tcttacgatt
    16501 tgactttcaa aatgaccaga ctgcgattcg acgatttcag agagaagctt tagcagcaac
    16561 agaactcgtt catccaaaca ttgttagtgt ttacgatgtt ggtgaagaag acaacatgca
    16621 gtatctggtg atggaatacg tcaaaggaat ggacttgaaa cgatatatcc aaacacatta
    16681 tcctgttcct tatgaaacag cagtaaatat tatgcagcag atcctttctg ctatttcttt
    16741 agctcataac catcaaatca tccaccgaga tttgaaaccc caaaacgtac tgatcgataa
    16801 tgaaggtgtt gtaaaaatca cggatttcgg gattgctatc gctttatctg aaacatcgat
    16861 cacgcagacg aatacgatgc taggatctgt tcattatctg tcccctgagc aggccagagg
    16921 aagtatggca acgaagcagt cagatattta tgctttaggg atcatccttt atgagatgtt
    16981 gacgggtagc gtaccgtttg atggtgaatc tgctgtaaca attgctttaa aacattttca
    17041 agatgatctt ccatctataa aagcacttga tccaaatgtg ccacaagcac tggaaaacgt
    17101 gattttacgg gcaacggcta aagaacctgc agaccgttat aagtcagccg aggaaatgtc
    17161 tgatgatctt tctacagtat tatcaccagc tcgtgcaaac gaagaaaaat ggcagcctca
    17221 tgtgatggat aatgaaacaa aagttatcac accccttacg gaagatgcac caatgcctga
    17281 ttcattcaaa tctatgcctc tgccaaaaga caagccagaa aacgaaacct taccagagga
    17341 ggaagaagtt ctgcctgatc ctaaaaagaa aggacagaaa aaatggtggc tgatcctatt
    17401 agctattctt gctgttttag gaatcggaac agctgtattc tttgcttctg gcgggcgagg
    17461 agaagttgtt gttcctgatg tttctgatct ttctgaagct tctgctagag aaacactcgc
    17521 aaaagcgaaa ttagaagtag caacaaaaac agaagaaatt gcagatgaca aaatcgaaga
    17581 aggtaatgta gtgaagacag acccggcagc gggaacgaca gtcaagcaaa aacgcgaagt
    17641 cactctgtat attagtagcg gcacgaaaaa aatcaaatta gatgattaca gtggtaaaag
    17701 ttatgaagat gccgaagcaa gtttgaaaga attagggttt tctgctgact tgattcaaaa
    17761 agaagaggaa ttcagttcag aggtagaaag cggaatgatc atttcgcaat ctccatcaga
    17821 agatacagaa gtcgatccta aaacagacac gattactttc acagtcagca aaggaccaga
    17881 aacagtgact ttgagtagtc atgtaggaga aaactacgaa actgttcgta attggcttgt
    17941 tacacaagga ttcaatatca ataatattag tgctacttat gactatagcg acactgtacc
    18001 atcaggttcg atcatcagtc aggatccagc ctcgggtgaa gttgtagcag aagaaactta
    18061 catcaatttt gtcgtaagcc aaggaacaga gcctaaattg aatgatatct ccggctatac
    18121 aaaaagcgaa gcacaaagtt atttagctag tgtcggtgca gaatatatcg ggcatgagac
    18181 ttccgaatat tcaaacacgg tggataaaga taaagtgatt cgtacagtac caggggcagg
    18241 aacgacaatt accaaaggaa tggttgtgaa cgtaatttac tctaaaggac cagatccagc
    18301 gtcttctagt tctgaaaaag actcttcaga cagttctagc agctcaagta gctccagtag
    18361 cacaagcaac tcttcaaaag aagaatcttc cagctccaca agttcatcag ctagcacctc
    18421 agaaagttca aaataagtgc tgaataaaat atatcagaag ggaatgcgac attgatttgt
    18481 tgcattctct tttttgtgtt tctatctctt ttgatcaatt ttcaagccat atactttgag
    18541 aagtttgcat ggaataacac ctactttcct cttcgtatgg taaaataaac aaaaacggag
    18601 gtggcactta tcaaaggaca aatcagaaaa gcaatcagcg gtttttacta tgtatacgca
    18661 aacggagaga cctatcaaac aagaggaaga ggaaatttcc ggaatcggaa aatcacgccg
    18721 cttgtcggag atcaggtgat ctttgagagt gaaaatcaaa cagacggcta tcttctggaa
    18781 atcttgccga gaaaaaatca attagttcgc ccaccagtca caaatgttga tcttggaatc
    18841 attgtcacaa gtatcgtaga gcctgcattt tcttataatc tacttgaccg ctttctagtt
    18901 accttggaat acgagtcgat cgaaccgatt atttttttga caaagaccga cttgtcagac
    18961 gacccagaaa aaatcgaaga gatcaagcaa acctatacta aaataggcta tccggtgatt
    19021 gtacccaatc atacgggtga tacgacagaa ctgatccgtt acttcccaga acggctatcg
    19081 gtctttatgg gtcagtctgg agcaggaaaa tcgacgctac tgaatcaaat atcaccggaa
    19141 ctgaatttag caacaggaga gatttctgat gcacttggaa gaggacggca tacaacacgt
    19201 catgtggaac tgattcctat ctatggcggg ttagtagcag atacaccagg atttagctcg
    19261 attgactttt tgacaattga agcaggagaa ctgccgagac aatttcctga atttgtagaa
    19321 gcttctgtcc attgcaaatt tagagagtgc atgcatgcta aagaacctgg ctgcgaagta
    19381 aaacatagag tagaaacagg tggtatcgca cagacacgtt acgacaatta tttgcaattc
    19441 ttacaagaaa tcgaaaaaag aaaaccaatg tatgcaaaaa aagaaggtaa acgaaaatag
    19501 gaggaaacaa gaatgaagat agcaccatcc attttgagtg cagattttgc caatttacaa
    19561 agagacattg agttagtgga aaaaggaggc gcagattaca tccacgtaga tgtaatggat
    19621 ggccgatttg tcccaaatat cacattaggg ccaaatattg ttcaggcaat ccgtcctgtt
    19681 actaaattac cgttagacgt ccatctgatg atcgttgatc cagaaaacta cattccagca
    19741 tttgcaaaag ctggagcaga tatcattact gtccacgtcg aagcaactcc tcatattcat
    19801 cgggcacttc aaatgacaaa aagcttagga gttaaagctg gagtagttat caatccagga
    19861 acaccagtct ccatgatcaa acatgttttg cctttggctg atcaagtact agtcatgact
    19921 gtcaatcctg ggttcggcgg acaaagcttt attgaagaaa cagtagaaaa aattacggaa
    19981 ctcgctgatc tacgtagaga gaacaattgg acttatgcaa tcgaagtaga tggcggaatc
    20041 gttccagaaa cagcaaaaat ctgtaaagat gcaggggcgg atgtcttcgt tgcaggatca
    20101 tatatctatg gagccgaaaa tccagtcgga caaatcgaac gattaaaaga ggcattagac
    20161 taaatgaaaa ttttattagc tgctggaggt aatccgcaaa actggccgca gtctctgctg
    20221 tccaaatacg atgcctatgt cgggatagac cgtggtgcgc tgtacctgct tcaagcaggc
    20281 tacccagtag atctggcagt gggtgatttt gattcattgt cgaacgaaga gcagaaggaa
    20341 gtatttgcaa aagccaaaga gtatacccgt tcccctgcag aaaaagacga tacagataca
    20401 cagttaggta tcttaaaaac atttgaacgt tacccgcaag cggaagtcac tttgattgga
    20461 gctacgggcg gaagattgga tcatctttta gccaatctct ggttaggtat ggaacctcgc
    20521 ttcaaaccct ttgcccatca gcttattatc agagattgtc aaaattattt gtcttattat
    20581 ttgcctggag agcatatagt aagacagata ccagatatga agtatttagc ttattgctgc
    20641 ttgactcccg tctcgaatct tacattaaaa ggcagcaagt atgtattgga aaataaagaa
    20701 gtagaatatc cgacatctta tgcaagtaac gaatttcttt cagataccgc agaattttct
    20761 ttttcagatg gtatgattgc agttattcaa agcaaagatt gatccataaa caaataaaaa
    20821 aatttgtctt tcaggctaaa aatgacagaa tctattattt gctaaaaaaa agcttaagag
    20881 gagcaactta ccttttgttt cacggcattt agtgataaaa ttgaacacaa gagtctaatt
    20941 aattcgaggt gtctttgtga agaagttcaa tcctaataaa aatattatta ttaccttggt
    21001 tattgttatc atcgtggtga cgatactaag tgtgaccgct gcgcagcgag cgaatgaagg
    21061 aaagcccaat ttcttccaat ccatcgttaa tgacagtgtt gcaatggttg ataaaacaat
    21121 ctctgcccca gtacggtggg tacgaaatgg agtagattct gtccataatc ttttttcaac
    21181 ttatgccgaa aatgagcggc taaaagaaaa aatcgatagc tatgatgacg tttcacagca
    21241 aaacaagaat tacgagagag aaatcgaagc gttgaaaaaa gaacttgagt tgaatcagac
    21301 gctgactagt tacgaaaaag taacggcaaa tgtcattact cgttcgcctg acagttggca
    21361 ggacatgctg atcgtagaca agggttctaa agacggtatc gaagcaaata tggcagtcat
    21421 gtcgcaaaaa ggattgattg gccgagtgat cgaggtcaat acggcttcgt ctaaaatcga
    21481 attactgtca tcctctaatg aaagctccaa tcattttcca gtacgggtat cttcggctaa
    21541 tggcgaagcg tttggtttgc ttaaaaacta tgatgaaaag ctccatgcct tagtggtgac
    21601 ccaattaact ggtgatacgg atatcaaaga aggggatgtt gtccagacat ccggtcttgg
    21661 agggaattct ccagctaact tgccgatcgg tacggttatt aaaacgaaac cagatagtta
    21721 tgggctggat cgggaagttt atgtgaaacc ttatgcagaa atgtatgacg tgtcagttgt
    21781 gacgattgtc caacggctag cggaggatta gtccatgttg aagaaagaaa caatgaagta
    21841 ctatctgcca atcgttttgt tctttttgat gttgatagat ggtcatttaa caagaatgct
    21901 aggggagtgg tcgaaaggca cctatatgtc aaatgcccac tttctgatat tggcattatt
    21961 atgttgcagt atggcgtttg aaaaacgtta tttactgatt accacgattg ttctcggggc
    22021 tatctatgac gcttactata ttggcgttat cggtatctat gcagtagctc tccctttaat
    22081 tgtatggttg atgtatgtaa tgaaagacgt tatccatgtc aacatcttta ctgaattttt
    22141 cagtatgatc atctttgtca cgggttatga attgtttacg atggtggtcc agttgatttt
    22201 taaattagca gtagtaaata acacgtattt tattacaagg tttttaggac ctacactgct
    22261 gttgaacatg attatatttg tattattcat ttttcccttt a
//
LOCUS       DAZKLA010000038        22160 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000038 DAZKLA010000000
VERSION     DAZKLA010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.038"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            230..2065
                     /gene="tdc"
                     /locus_tag="ACG85N_001234"
     CDS             230..2065
                     /gene="tdc"
                     /locus_tag="ACG85N_001234"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0224018.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2370..3848
                     /locus_tag="ACG85N_001235"
     CDS             2370..3848
                     /locus_tag="ACG85N_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0224019.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3814..5145
                     /locus_tag="ACG85N_001236"
     CDS             3814..5145
                     /locus_tag="ACG85N_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0224020.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5259..7811
                     /locus_tag="ACG85N_001237"
     CDS             5259..7811
                     /locus_tag="ACG85N_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFQ0224021.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7818..9257)
                     /locus_tag="ACG85N_001238"
     CDS             complement(7818..9257)
                     /locus_tag="ACG85N_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0224022.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9232..9414
                     /locus_tag="ACG85N_001239"
     CDS             9232..9414
                     /locus_tag="ACG85N_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224023.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9603..9965
                     /locus_tag="ACG85N_001240"
     CDS             9603..9965
                     /locus_tag="ACG85N_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFQ0224024.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10152..11009)
                     /locus_tag="ACG85N_001241"
     CDS             complement(10152..11009)
                     /locus_tag="ACG85N_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0224025.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11129..12040
                     /locus_tag="ACG85N_001242"
     CDS             11129..12040
                     /locus_tag="ACG85N_001242"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0224026.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12047..13693
                     /gene="mdcA"
                     /locus_tag="ACG85N_001243"
     CDS             12047..13693
                     /gene="mdcA"
                     /locus_tag="ACG85N_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFQ0224027.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13795..14094
                     /locus_tag="ACG85N_001244"
     CDS             13795..14094
                     /locus_tag="ACG85N_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFQ0224028.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14084..15760
                     /gene="mdcD"
                     /locus_tag="ACG85N_001245"
     CDS             14084..15760
                     /gene="mdcD"
                     /locus_tag="ACG85N_001245"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFQ0224029.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15764..16357
                     /locus_tag="ACG85N_001246"
     CDS             15764..16357
                     /locus_tag="ACG85N_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFQ0224030.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16381..17457
                     /locus_tag="ACG85N_001247"
     CDS             16381..17457
                     /locus_tag="ACG85N_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0224031.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17482..18351
                     /gene="mdcB"
                     /locus_tag="ACG85N_001248"
     CDS             17482..18351
                     /gene="mdcB"
                     /locus_tag="ACG85N_001248"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFQ0224032.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18462..19226
                     /locus_tag="ACG85N_001249"
     CDS             18462..19226
                     /locus_tag="ACG85N_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFQ0224033.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19715..21808
                     /locus_tag="ACG85N_001250"
     CDS             19715..21808
                     /locus_tag="ACG85N_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0224034.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21833..22135
                     /locus_tag="ACG85N_001251"
     CDS             21833..22135
                     /locus_tag="ACG85N_001251"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0224035.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7177 a         3786 c         4721 g         6476 t
ORIGIN      
        1 ctttttttag gttatctatt ttagaagact gatttattga aaaattgttc gcttttttgc
       61 gttgaattat cagaaaaatc cgactttatt tatttttgtt tgtaaaaatc tacgtggatt
      121 tgatgttata aaaaaaaaat tctcattttt ggcgctatag tgaagaggtg ttacagataa
      181 acacatgtat ctgcaagaca ttgaccaaca aaatatggag ggaagaaaaa tgaaagatat
      241 ggatatcaag gccgtcttta taggcgacaa agctgaaaat ggaccagttt ataaaatgtt
      301 gttgaacaag atggtagatg aacatcttgg atggagagaa aattatattc cttcagatat
      361 gcctgcaatt agcgaaggag ataagctcac tccagattat ctagccaccc gcgaccatat
      421 gatagaggtc ttggatgaag tcagtcagcg attacgcgca ggatcgatcc catggcattc
      481 agcaggccgt tactggggac aaatgaatgc tgaaacattg atgcctgctt tattagctta
      541 caactatgcg atgctgtgga acccaaataa tgttgcattg gaatcttcta tggcaacttc
      601 gcaaatggaa gcagaagtag gacaggattt tgcttcatta ttcaatatga cagacggatg
      661 gggacacatt gcagcagatg gttctattgc taatcttgaa ggattatggt acgcccgttg
      721 catcaaatcg attcctttag ctgtcaagga agtgctgcct gaaaaagtga aaaaaatgtc
      781 tgagtgggaa cttttgaatt tatctgttga agaaattttg gaaatgaccg aaagctttac
      841 ggacgaagaa atggatgagg tcaaagcagc ttcttcccgt agcggaaaaa atatccaaag
      901 attagggaaa tggctggtcc ctcaaacaaa acactattct tggatgaagg cgttagatat
      961 ttgcggagtc ggcttagatc agatggttgc tatcccagtt caagaggatt atcgaatgga
     1021 tattaatgct ttggaaaaaa caattcgtga attagctgga caaaaaatcc caattctagg
     1081 tgtggttgct gtcgtaggaa cgacagaaga aggtcaagta gacagtgtag ataaaatcgt
     1141 ccaattaaga gaaaggttaa aagatgaagg gatctatttc tatttacatg ttgatgccgc
     1201 atatggcggc tatgcgcgtt cactgtttct aaacgaagca ggagaatttg tgccgtatgc
     1261 ttccttagct gaattctttg aagaacatca tgttttccac cactgcgtaa cgattgacaa
     1321 agaggtatat gaagggttca gagctatttc agaagcggat tctgtcacga tagatcctca
     1381 taagatgggc tatgtccctt atgctgctgg aggaatcgta atcaaacata aaaatatgag
     1441 aaatatcatt tcttatttcg cgccatatgt gtttgaaaaa tcagtaaagg ctccagatat
     1501 gttaggagct tatattttgg aaggatcaaa agctggagca accgccgccg cagtctggac
     1561 agcacatcgt gtattgccat tgaatgtgac cggatatggt caattgattg gggcttcaat
     1621 cgaggcagct caaagattca gagaatttct cgatcatctg acttttactg ttaaaggaaa
     1681 aacaatcgaa gtctatccat tgaatcatcc tgattttaat atggtcaatt gggtattcaa
     1741 agaacaaggc tgtacagatt tgaacgctat caatgaatta aatgaaaaaa tgttcgatcg
     1801 gtcttcttat atggatggcg atgtttatgg tgaacggttt attacttctc atactacatt
     1861 tacacaagaa gattacggtg attctccgat tcgttttgtt gaaagaatgg gtctaacgaa
     1921 agaagaatgg aaaaaagaac agaaaatcac tctgctacgt gcggctatca tgactcctta
     1981 tttgaatgat gatcgaattt tcaattttta tacaaaaaaa atcgccaaag caatggaaaa
     2041 gaaactaaac gaaatcatcc aatagaactg atttttgtgt gaaagaataa caagagacat
     2101 atggagaaca gacaattatt tgcttcatca aaacaattgt atttccccct gtttttcata
     2161 tattcctcgc caagatattc ttttttataa aacgttctat aagttggatc gttcgtttca
     2221 tgtatcaaac ccagttcact aagatcgttc aatggatgtt tcggtgatac tgggttttgt
     2281 actgaatcga tgaaaccttg tcatttctgt tcttgaacaa aaataaaaga aaacaaaaac
     2341 atatgtcgct attgaaagaa ggaataaaga tgaaagatac tggttcatca aagcaaatca
     2401 cgaccatgac ctttatcgga atgacctgcg cccttgtagc aagtgtacga aatattccag
     2461 atgtagcagc aacgagctgg acgatgattt tttacatgct ggttgcagta ttattgtacg
     2521 cttttccaat ctcattgatc tctggtgaat ttgctggaat gtttccccaa aaaggtggtc
     2581 cagaactttg ggtatcgaat gctctaggaa aaaaatgggg gttcgttgtc tcatggctgt
     2641 tatgggtcca aatgtttcct gggatggtga tggtcgcttc agcactcgct ccgttgtttg
     2701 gtaatattat cgataatgtg ccgcttggtt tgaacagcaa atttacatta gttgtcattt
     2761 tagtcgttta ttggatcatc acttttttga atttgaaatt tgatatggca aaaatcggcg
     2821 gaaaagtcgg tgtttggtta ggattgtacg tgcctttgac gatgatgctc ttattagggt
     2881 ttgctgcgtg gatcaaagtt gggatcgtac caaccggaac attaggcagt ttttcatggg
     2941 acaaattaat accagatact acgactgcat cctcatttgt ttattttgcg ccgatcatgt
     3001 ttatttttac tggtatcgaa atgtcctctg tgtatatcac tcgattggaa aatccagtta
     3061 aaacgtatat tcgcggagta tttgccgcgt taatttttat atttttcgtc aatattttga
     3121 atgctttagt tgtagcaaat gtcgtaccaa aaggacaaat ggaattgaat aatatcgcgc
     3181 aatctatttc gatttattgc caaattcttg gtttgccaca tcttattgtg aatctgttta
     3241 gtttacttgt cttcattggt gtagcagtcc aattatctgc ttgggcctca ggaccagcaa
     3301 agacagtgac agaaagtgct agaaaaggtg cgtatccacc aaaattcaat ttttggaaga
     3361 cgaatcaatt tgacgtatcc aaatccgtta ttttgactca gtctgtcatt atctcaatct
     3421 ttgcactttt ttatttgctg attcctggcg tcaatcaagc atttttgatg ctcgtaaatt
     3481 caactactgt tatctactgt atcgtctatg tcatcatggg gatcgcagtt ttgagattgc
     3541 gttatacaca tgcaaagttg aatcgaccat ttagaattgg aaaaaaagag aaaaagagca
     3601 atcttggagt ttggactgtc gttattgttt tatttgcggc aatcggattt tctgtcggac
     3661 tgacgatgaa agctggaaca tggatcaatc taattgcagt cacagccatc agtgtcattt
     3721 tgtttgtagt accactctgt attgaaaaaa taaagaaacc ttcttgggaa caggaagtcg
     3781 ccatgctgct tgaaaaagaa aaaggtgaac ttgatggaaa aaccggcaat gaaacggact
     3841 ctaactaatc gaaatatcca gttaatcgca ttaggtggcg caattggaac aggattattt
     3901 ttaggttcag caaaggcaat tgcatcagca gggccagctg ttattctcgt ttatttgata
     3961 tgcggtttgg ctatttatgg tttgatgcgg gccatgggtg atttattttt agcaaatagt
     4021 acatttaact ctatcagtga tttcgttgac tattatttag gcaaaaagtg ggcatttttt
     4081 gtcaattgga cttattggct ttgctggctt ggagctggac ttgcggaatt aacagcaata
     4141 ggattgtata tccatttctg gtttgtacat gtaccagctt tgataagcgg aggtagcgca
     4201 ctaatgatct taatggcagt caaccttgtt gcagtcaagt ggtttggtga attggaatct
     4261 tttttctcaa tcatcaagat cttggggatt ttccttttta tcttattggg tataggattg
     4321 agtgtctcag attttcatac ccagtctatc ggaatcaaag aaaattttgg acagatcgat
     4381 gaattactac cgtttggctt tgctggaatg gtagtctcat ttcctatggt tttattcagt
     4441 tttgctggaa ttgaaatgat cggcttaacc gttggcgaga caagtgatcc gcaaaagaat
     4501 ttgaaaaaag caatcaactg tctgccttgg cgtattctct ttttctatat aggaacgatt
     4561 ttgtcgattt tatttgttgt tccatggcat tgtttagatt taaaagaaag tcctttagtg
     4621 actatgctgc aagtgatcca ttgccaaggc ggagcagcaa ttgtcaatct aatgattttg
     4681 gttgcttctt tgtcctcagc aaatagtgca gtctacagta ctagcaggat tttatatcaa
     4741 acagcaaaag aaaaaagttt gccccattgg ttttgtgcgc tttctaagaa aggcgtacct
     4801 ttacacggta ttttaattac aggaatgtta ttttttgccg gtctttgtct acattttttt
     4861 gtagctgata gtcgattgtt ttttcagctt ttggcaggca tgacaacgag ttgttttcta
     4921 tttgtgtggt cttcgatctt gtgtgcacat gttcaattta ttaaagaaaa gaaacggaac
     4981 actacacaaa gatataaaga tcttgctttg ttggtgtttt ttacttcaat tgctttcagc
     5041 ttgttattcg aaagcacgac acgctttatt ccttttttat tggttatctg gtttgtattc
     5101 ttaaattttg tatataaaag aataagcaat agaaatatca gttaggccca gatacatttt
     5161 gcaaaacgaa ttatttcaga cccccctgaa ataatcgata cgcctgaaaa aacttccctg
     5221 cgtataataa cagtatcaat taaacaggag gggataggat gaactggtac agattgacgg
     5281 tagaagatgt attgaaaaac tatcaaacat caacagaagg cttgtcgcaa gaagaacgac
     5341 agaaacgcct gactgatcaa ggatataatg aaataaaagc aaggcagcag acaaaacgat
     5401 ggaaaaaaat agcaaagcat tttactgatc tgctgatgat cgtgttgatc attgcctctt
     5461 ttttgaaatt tgcttctagt gaatatattg aaggaagtat tattttattc gtagtgatcg
     5521 tcaatggact cgttagttac tggcaagaaa gaaaagcgga agaatcattg aatggtttga
     5581 agcagctgat gggacaagaa gcgatagtac tatcaaacgg gatgcaagaa aaaatccctg
     5641 cgaaagaact ggtaaaagga gatgtcattg tcttacgtgc cggagatgtc gttccagcag
     5701 atatccgatt aatcgaagca catgatttat tagtggaaga atcgattttg acgggagaat
     5761 cagaagcatc tgaaaaaagt catgccgttt tgacagaaga agaatcgatc ggcgaccaga
     5821 aaaatatggg tttttcagga acgcttgtac aatctggttc tgcagtcggt gtcgttgtag
     5881 aaacaggaaa cacgacagaa atcggtaaaa tcaatcaagc attgcaatcg gtcgaacaac
     5941 aaacgacgcc tctgattaaa aaaatcaacc gattaaacaa acaaattttt caaggcattc
     6001 tttgtttgag cctattctta gttattttta cgacatttcg atatggtatg gattggcata
     6061 tccttctttc ggctacgatt gctttggtgg tgtcaatggt acccgaagga ttgccagctg
     6121 ttttgaccat gattctatcc gttggagtac atgaaatggc aaaagaaaag gcgatcatca
     6181 aaggacttcc ttcggtagaa acattaggtt ctatgacagt gatctgttca gataagacag
     6241 gaacattgac aaaaaatgaa atgacagttg tggatgtagc tgcaaaagaa gaagcctgcg
     6301 tgttgtccat tatgaaaaac tgtcaggaat taaaaacaaa agaagaacag aaaacagaaa
     6361 atttaagtgg caatccgaca gaagttgcgc tgattcgaca cacagaaaca gccaatctac
     6421 ctttgaaaaa aatagaagca aagattccgt tcagttctga atacaaatat atggcaacga
     6481 tgcatgcgga ggaaaaagga gcggtgattt acgtcaaagg tgcaccagaa gtattatttg
     6541 ctaaatcaac tctttcttct gcagaacaag aagcatggag tcagacagct gcagaatttg
     6601 cgagaaaagg tcagcgtgtt ttaggatttg cttataaaaa agtagattcg aaacaggaac
     6661 tcacgcatga gactttaacc caattaactt ttgcagggat cgccggattg atcgatccgc
     6721 caaaagaaag tgcagtcaaa gctgtaaagg aatgccaaca agcagggatc tcagtgaaaa
     6781 tgattacagg ggatcacaaa gacacagcaa aagcgattgc ggaacaaatt ggcttaaaac
     6841 atactgccaa agttttagaa gggatcgatt tggatcttat gtctgatgaa gagttgattc
     6901 agcaagtgcc aatagtcgat gtctttgctc gaacgacacc agaacataaa ctgcgaattg
     6961 ttaaagcttt gcagaaaaat ggagaaatcg tcggaatgac cggagacggc gtaaatgatg
     7021 cacctgcttt gaaaagatcc gatgtaggaa ttgctatggg aatcaaaggt agtgaagtaa
     7081 gcaaacaggc agctgatatg gttttaggag atgataattt ccatacgatt gcaaaagccg
     7141 taaaagaggg ccggcggatt ttggataatt tgcaaaaaac catcaatttc ttcttgccaa
     7201 ctgcattagc tcaaggattg atcttgattt gggcacttat gctgaatcgc ccgctacctt
     7261 tgtccccagt acagatttta tgggtcaata tggtaacgac aatcacttta tcttatgcac
     7321 tagggttcga gtcgccagat ccagaaatca tgaagcgccc accaagagat ccaaagcaag
     7381 gaatcctatc tggttatcat ttgtttcgga tcatctatgt ctccctactg atcatggttc
     7441 ctgcctattt attggctatg cagtttgaag ggcaaagctt acagcaaacc attcttctgc
     7501 aaaatatcgt cttagctcaa gctgtttaca tgatcaattg cagagagttg tccaagccgt
     7561 cattgaacca aggtcttttg caaaacaaag cgctttttct ttcattaggt atccttgctt
     7621 tattgcaagg gatggttatt tttctaccag tcggccagca gctgatcggt acagtaagtt
     7681 taacgatgac acaacagtta ttgattggag caaatgtgat tcttctattc ttagttgtag
     7741 aaatagaaaa agggatcatg aacaaactgt ttcgcaaaaa agaaaaaaca gctagagaat
     7801 acagtcgcta gccgttacta taaaaaagtt ttgaaagtga gttcagtcaa acgattgagc
     7861 tcatttttcg ttggaataga agaaatgtat acaataggga tatctagaga atctagaggg
     7921 aaattggaaa tcaagatatc tgttttttca aaagaaatag tggaaagatc ggttagttcg
     7981 ataggtaaga agcagacacg ttttcctaag taatctttta attcctgctg caagaacgcc
     8041 ttccatgaag gttctccttg gaaaataaaa tagacattga acaaattcgg tttgatagaa
     8101 aaaatcgttt gttggatcag tagagacaag agttggagaa agtaatttgg ttcattgatt
     8161 tctatttctt tttttagatc gttcagtaaa ttctctttct gttgatacaa gtgtgcatat
     8221 tgctgatcat atgtttctaa aaaacgatgg tattgacggt ccaatgcttc aatcaatatc
     8281 ggcgattcat gatattttaa tatgaggaga gccaagttat cagtcagaga tgtttttttt
     8341 agttgatcaa gagacagctc tgaagctagt tcctctacaa acgtttcgag gacctctaaa
     8401 aatggagaat gatgcaggca gcgagcggtt tcttgtccat agttattgtt gtaattccaa
     8461 ctttccagaa agcagaaaga agcaaaagaa agctcagtgt ctttaagata aataccttcc
     8521 gttaaataca attttttcaa tgcagagtca tacagggagg agagtgtgct gttggtatgg
     8581 atagaagaat ctaatcggca attcgctttt atacggatcg tattgataaa gaaccaacat
     8641 gttccgaaaa tctcttctgt ttcaaccgct agttcagttt gactgaggtt tcttaaaaat
     8701 tgaaaataat gggattcatg gatcgaataa tcctcgaaaa aatagttgtt atgagtgaaa
     8761 ggaatcagca atcgatgata aaaagtacga atcgcacgct cttttccaag aatacaagct
     8821 gtatatttcg tcaaatcgat ctgcaagtcg taacttgcta aacgctgatt gattttagtc
     8881 acatgtcttt tcaatgtccc ataagaaagc ccatggtcgg ctagaaaatc agggacagca
     8941 actttttttg taaaaagcag atttttcagc agttcgacac tgactgattg tttgaaaaaa
     9001 atctcccaaa ggtcatttgt taataaattt tgctggtttt gcaaactgat tcctgcttga
     9061 tcgctgatga tgtcccagcc ttcgggcagc tgagagcgaa tagattgaag gtcagaaaaa
     9121 atcgttcgtt cggttgtccc tatgatttca gctaattcat ttacggttat ctttgtatga
     9181 ttcagcaatt gttctactaa agaaacatga cgtcgaatat ctttttcggt aatgattttt
     9241 ttcattagtg aatacatcaa tcagactccc ttctattcta agaatacgga gaaaataaac
     9301 ggtggtcaag tttcaactga tttttcaatc attgtttatt tttctggagc atttactgat
     9361 gataagaatt tcatcatgaa cttgaaaaat gataccacta aaatcaaaaa ataggtgggc
     9421 aaacaataat aaacatttta atagtagatg gtagcaagac caacacaggc ggattgaaca
     9481 tttaagagtt gataaaaaag aaaaatatcg aagtatttga ttagcaacta cttgtattca
     9541 tttttctatt cttacgtggt atctttgaat aaagcgaatc aataagaaag agatggaagc
     9601 agatgctaac gaaaaaagaa ctaatcgatc acatcaatga aacatacaat ttgtctccag
     9661 aatatatttt taaaaaattt cctgattatt gcgtattcag acatcaaaaa agcaaaaaat
     9721 ggtttggggt cctgatgtct gttccagaag aaaaaatcta tgatgatgca agtgaccata
     9781 ggatcatcaa tattcttgat ttgaaggtcg atcctgaact tggcggaatt ttgcgaggaa
     9841 aagaggggta ttttccagcc tatcacatga ataaagaaca ttggatctct attgatctac
     9901 gtaaaataga aaagctagat gaactagcag atttgattga tggaagtttt aaatcaacgc
     9961 aatgaatgtc taggacaata gagaagaggt tgtgacagaa atgtttagct tcaagaaata
    10021 agaaggaatt tacgaaaatt gcttctcaaa tttttgggaa atttcggctt attttctaag
    10081 aaattacttc tggaacaccg tttgttcgaa aaaagagctg cgacaagaat tttgtcccag
    10141 ctcttttttt tttattttcc taaataaaat tcccgcatcg tttttgcaat cattttgatc
    10201 aaaggatgat cttctgtgaa gctagaagat aaataaattg ttcgtgtcaa gggcaaatct
    10261 ttgatttccc gcaagacgaa aggatcattt ttatcgtaag accaagaaac ttttgggaaa
    10321 aagctaacag ctaatccttg cttgacaata gaacgtaatg tcgcaggatc atctgtcgtc
    10381 gaactgatcg tcaattcgat acttttcgct tcaagtgctg catccagtgt atgacggaag
    10441 gcgttgctct ttgacagcat caagatgggg gtgcgttgga tatcttctaa ttgcagatgt
    10501 tcttttttag caagaggatg tttcttagga atggcgacat aaacaggttc tttcatgata
    10561 ggaatattgg tcatgccagg cttcatctcc gaagtgatgt acagtacagg atcggtaata
    10621 tcatctggta aatgatgttg catgatcgtt aattggatat caggatgctt ttccataaaa
    10681 attcggacca agttcggaat agaaacagag gctacctcta aatacaatat gacggaagta
    10741 acgatcttct cattgaccga agacaataat ttgattcctt cttctaatgt gtctaatgtc
    10801 ttgactgcat atggataaaa ttcttctgca taaacggtcg tctcaatgtt tctgccgcta
    10861 tggtaaaaca aagggacacc taattcgttt tccaaatgtt tgatcgcacg gctcaaggca
    10921 ggttgagaaa taaataattc tttggctgct tgggtaacat tttttgtttc gaatactttt
    10981 ttaaaatact gcagatgaag caatttcata attatccatc ccatccatac ctttttgtta
    11041 tggttttttc tttttttggc attttttatt atagcagaaa ttgactaaga tagcagtgga
    11101 aaaagtaagt gctgaggagg cgaggctcat gagtacaggt ttcttatttc ctggacaagg
    11161 cagtcagaag ttagggatgt tagcggatat ccctgaaatg tacatggaac gtttagaaaa
    11221 tgtgacgggg tatcggttag ttgataaaga agaaaactat caagatacgg tttttatcca
    11281 gctagcatta ctgacaaaag cagtatttta tttagatgaa atggaaaaaa acggtattca
    11341 gcctgatatt gcagcaggac attcgattgg tgctttttgt gcagctgttg cttgtcgatc
    11401 tttgagattt gagaatgcag cggaactagt ctatcaccgc gcagtcttaa tgaaagaagc
    11461 ttatccaagc ggttatgcaa tgggcgtagt agtaggtgtg acgagaagcg aagctgagga
    11521 aattgtagca aaaacatttg atcctgacca tccagtttat ctgtcaaatg aaaattgtcc
    11581 gatgcaacat acgttatctg ggagtatcga aggtttatcc gttacgttgg cagaagccaa
    11641 acggcaccaa gcgcaaacag cgaagctgct gaaagtccct gtgccatctc actgtctctt
    11701 gatgaatgat acggtcaaga aattcaagcc gtatatgcag tctgtccatc tatctgatgc
    11761 tagatgtttg tatataaaaa atacggatgg aagagccact caggacgcag aagagattcg
    11821 taaagatttg ttggaaaacc tagctttccc tgtgcagtgg aaccaaatga tggacttggc
    11881 aaaagaatta ggaatggatc tgacgattga atttcccccc ggcaatactt tgacaaagct
    11941 gattcataca aaatttggtg tagataaagg gatccgtacg atcaatctag accaacatgg
    12001 tattgatgac gccacgtttc tatatcaaaa atggagatga ctaattatgg aaaaaccaaa
    12061 ccgcaactgg gcttctaaac gcgaaaaaaa agcgcagcga ttggaaaaaa tcagcaactt
    12121 gctcaatggc aagtatgtag aaactgaaaa aatcgtagaa gtaatggaaa aactgattgc
    12181 tcccggcgac aaagttgtat tagaaggcga caatcaaaaa caagcaagtt ttctttcaga
    12241 aagtcttgaa aaagtagatg cagaaaaagt gaatgatctg catttgatca tgtctagtat
    12301 ctcacgacca gaacatttga atatttttga aaaaggaatc gcaagcaaga tcgatttctc
    12361 ttatgctggt gcacaaagct tgcgtgtcgc ccaaatgatt gaagacggca caatgaaatt
    12421 aggtgatatc catacttatt tggaacttta tggacgctta ttcatcgatt tgattccaaa
    12481 tgttgtttta gtagctgcag ataaagcaga taaagacggc aacttgtaca caggtttcaa
    12541 tacggaagaa acaccaacaa tcatcgaagc tgcagctttc aaagatggta tcgtgatcgt
    12601 tcaagttaac gaagttgtcg attctttacc acgtgtagat atccctggtg actgggtaga
    12661 cgttgtcgtg aaagcagata aaccttatca attagaagct ttattcacac gagatcctca
    12721 aaacatcaca gaattgcaaa ttctaatggc aatgatggca atcaaaggaa tctacgcaga
    12781 acacggcgta caatctttga atcacggtat tggattcaat acagcagcta tcgaattgtt
    12841 actgccaact tatggtgaat cactagggtt aaaagggaaa atcgcgaaga actgggtatt
    12901 gaacccacat ccaacgatga ttccagccat cgaatcaggt tgggttgaaa gcatccatag
    12961 ttttggtggc gaagttggta tggaaaaata tattgctgct cgcccagatg tcttttttgt
    13021 cggtaaagac ggcacgatgc gcagtaaccg aacattatca caagctgcag gacaatatgc
    13081 catcgatatg tttatcggtt caaccttgca attagattac gaaggaaact cttctaccgt
    13141 aacaaaagga cgtttatctg gttttggcgg tgcgcctaac atgggacata atcctggcgg
    13201 tcgtcgtcat tctacaccag catggcaatc acttcgcgac aatgatgatc cattagggaa
    13261 aggccaaaaa ttagttgttc aaatggttga aacatatggt tcaaataaaa aaccagtctt
    13321 tgtcgaagaa ttagatgcac tagctgttca aaaacaagca ggcttagcca atgcaccagt
    13381 gatgatttat agcgaagata ctacacatat cgtgacagaa gaaggaattg cttatttgta
    13441 caaagcaaaa acaaaagaag aacgacaagc agcaattgca gcaattggcg gtgttacacc
    13501 tcttggaatg actagcacga aagaatcact agatagcttg cgtgcagccg gcatcgtgaa
    13561 attaccagaa gatctaggca tcaaacgtag tgaagcgaaa cgttcattat tagctgcaca
    13621 aaccatcgat gatttgatcg aatggtcaga tggcctgtat gaaccaccaa tgaaattcag
    13681 aacttggtca taaaattggc gagaaaaacc ttttgtcgcc atttacccag atggaagaag
    13741 aaagacgaat aagcagaacg tgtatgctct gttttacaaa aaggggagag atccatggaa
    13801 caattagctt ataaatatcc aacgaaacag ccgatcaata agaaaatcca tgtaggagtg
    13861 gtcggttcag gagatttgga aatcttgatg tttccaacag aaaaaacaga atctactgta
    13921 aaaatttgta caggaagcga tggcttcggc gaagtatgga acaatgtatt aacgagattt
    13981 tttgatcgtt atccaatttc agcagatatc gtgatcaatg acttcggtgc tacaccagga
    14041 gttgtgtacc tacgattgac gcaagcaatg gaggaacttt cagatgaaaa atagttttgt
    14101 agaattaaac gccagagaac gagtgaacgc tctacttgac aatggacgtg aattgattga
    14161 tccattcgat caaatcatcg cgccaagttt accagcacaa ggaatcgtcc ctgaaagtga
    14221 tgacgggatc gttgtcagcc gtggcacgat caatggcaaa caagcagtag taatcgctat
    14281 ggaaggaaaa ttccaaggtg gcggtatcgg tgaagtttcc ggtgcaaaaa tcgttgctgc
    14341 acttgaacat gtgttagaag aaaataaaaa aggaaatgag atctttccag tcattgtact
    14401 agatacaggt ggtgttcgtt tgcaagaagc aaactacgga ctgttatcta tttcagaaat
    14461 cggaaatgta atcattgctc taaaacaata tgtaccagtc atcggtttga ttccaggacg
    14521 tgtaggatca tttggcggta tgtcgattac ttcagcatta atgagttatt taatcgcaac
    14581 aaaaaaagcg agagtcggtt tgaatggacc agaagttatt gaacaagaag caggcgttcg
    14641 agaatttgat tctagtgaca aagacttgat ttggaataca ttaggatctc gtcaacgggt
    14701 agaatcaaaa atcattgatg aattagtgac tgatagtgta gaagcaatca aagctgctgt
    14761 catcgcagca atggacgaac gaaaagacac gcatcgcagt gaaagctcag cattttattt
    14821 atcattactt gattctttag atctttcaaa accaatggcc attgacgatt acaatgaagc
    14881 aattgctaac catcaagcaa aatcaattga cttaccagaa gtagcagaag gaaatgaacc
    14941 agctgcagca agtgttggtt acgattggtt tgcggcacta actggcatga aaaatccaac
    15001 ttcttctatt agtactgtgt attatggtaa atcaagtaaa ttcatagaag aagccatcgt
    15061 gacatcaatc gtacctgatc ctaaaaaccc gatgtaccgt gtacgaaacg gtgaagttgg
    15121 tttagttgaa ggtttccgca tgggacaaat tttggatcac gtttacgaag aagataaaga
    15181 caaaaaagtg aaacggccaa tcatcgttgt gatcgatgta ccaagccaag catacggcta
    15241 taacgaagaa ttgatcggta tccatgtggc tttggctaat agtgctgctg cttatgcaaa
    15301 actccgtcaa gctggacatc cagttattgg attgattgca ggaaatgcta tttcaggtgc
    15361 atttttagca catggattac aatcaagccg cttgattgct ttaaacaata aagaaattac
    15421 cgtccaagcg atgtctaaag aaagtgctgc acgtgtgaca aaacgttcgg ttgctgaaat
    15481 cgaagcagct gctgaaaaag ttccagctat cgcttacgat atcaataatt atacaaaact
    15541 aggagcgatc tatcatttcc tagaagatat caccgaccaa gctgcttcag aacaaaatgt
    15601 cagcaaagtc atcgaagcaa tcaatgaagc cattcaagat gtccgcaata caaatgacac
    15661 gatgctgaca aatcgataca caaacgaaac agccaaaaca ttcggacgtg tcgaaacaaa
    15721 taaagtccgt gccaaaatgg acgaagaatg ggatgcataa aaaatgattc aagcacatga
    15781 tattgtcctt tttgataaac atgaaataga agggttcacc ttgcctgaat gggtcgttct
    15841 aactgaaaag atgttcggaa cagttcgtcg tgcgtcgacg gatcacgcag aaatactacc
    15901 gattggttta cgtggacctg cccgcaatca gagatggtca acagaaatct caaaacatgc
    15961 cgtcaaacaa gtcattcatc cgtgggacat catcgaacaa gaatcgtata gacaagagaa
    16021 aatcatggat tatcctgttt atcagcaatt tcatgctgct agaaacatat tatcaggatg
    16081 taaatgggga atcggcggca gcttaggatt tgaattatcg actgggattc cggcagtaaa
    16141 agaaaccagt gattttgatt tgctgctgta cgcaaattca ccgattgaat tgccaattca
    16201 ggtaattcaa tcgcatcctg ctttttttga gcaattcgat acccaagtag tgactcggca
    16261 aggcggtttt tcactaaaag agtatttgag agagccagag aaaaaaattc tattgaaaac
    16321 agtcgaagga cctaaattaa ccaaagaact ttggtaataa agaaaaagag agagaaagag
    16381 atgtttgaaa acaaaaaaat caaaatcaac gtaagtgact tccagaaagt atcagctgta
    16441 acggctgtta tgatgcaaac gatattgagc tttgctttgg caaacagcca cagcgcaaca
    16501 aatacagctt ttcttggtac cttttacgtc atcgttaaat acacagcacc aatgtttatt
    16561 tttgcaattg tttataatat ggtaaaaaca agtcagcatt tgtcttattg ggaatttttg
    16621 aaagaaaagt tttttgaact agtcgttccg tatcttctat ggacgacggc ttatctttgg
    16681 ctatttccag cagtccaaca acaaactccg tatacggata ctgcaagctt tttactgaaa
    16741 tatgtcacag gtgatggagc gcctcattta tggtataccg ttatgatgct gcagatccaa
    16801 ttgttcatgc cgttttttgt ttggctcggt tacaaagtat ttgctaataa aaaatatgta
    16861 tggccagtat tgattggttc aacgatttta tatgtcgcat ggtatctgtt ctatcaaaca
    16921 caagtattga ctgggcctta tcatgacagc tggtacttat tagatcggtt tgttgctagt
    16981 ttcatgattt atggggtagc cgcatttgtt tatcacgaaa aagtctatca atatttggac
    17041 agagtacgtt atcttttctt acctgttgcc ttagttatcg cgttcttttc tgtccgttca
    17101 ttgctcgcac atccaggaga cttgagcttt gcgaatgcac cttacttgaa tacgatccaa
    17161 agtttataca gtttagtcat catctttgct gtcttcatag gcgcttccaa aatgattgta
    17221 aacgattcgc caaaattgcc attgttcaaa tggttgtctg tttatgctta tcgtacgtat
    17281 ttagccaatg tatttgtttt ccaagtactg ttattattgt ttaaagatag ttggctgcag
    17341 ttaccttcag gagtcatgat tcttgtagct tatctaatga cggcaagttg tgcatttgct
    17401 ttaagctggt tacttcatat catttgggta gctatcaaaa aaggtttcag caagtaatag
    17461 taggaagaag gagtgagtgt catgataaat gacgaacgaa aaaaagcagt gactcagcaa
    17521 ctcgctgcgt tggtagagga agcattgatc gcagaagtaa cattaagtcc aaaacctgga
    17581 ttagtggatg cactggatgc gggtgcacat tccgatatgg actatgcttt atttcttaag
    17641 tctgctaaga cgctaactcc tttttttgaa gaaatggctc aaatagcatg gcatcatgct
    17701 attgatcagg agttaagaga aagaattgct gagattgggc gaaaagcgga aaaggcaatg
    17761 ctgattgcta caaatggcat caatacccat aaaggagcga tttgggcaat ggggctattg
    17821 atcagtgtca ttgcacaaaa aatcagtatg gaacagcctt tttctttatc gaaaatactt
    17881 gatgatgtca gcaagcttgc tgcttttcct gatacaaaat atcgtcataa aaaaaccact
    17941 catggtcata gggtgaagca aaaatatggc gtaaatggtg catatgaaga agcgatatta
    18001 gggtatccgc atatcaggct tgcaattcaa gcagctaagg agctagataa tgattcatat
    18061 gtacagaaac aaatgcatat gttgttagtt ttgatgagtt cgctagatga cacatgcgta
    18121 ttgcatcgca gtgatcagaa aacgttagtg gaaatgcaaa aacttgctca tgctgctagt
    18181 caaagtgctt taccaaatcg tccatttaca gcactgttgc acttttgtca ggaaaaccaa
    18241 atctctcctg gaggaagcgc agatctcttg tctgcaagct tgttcatgct gaagatagag
    18301 gaattatgga ctgccgacac atacatttgt ccgatgttac tcaatgacta aataagacaa
    18361 aattgctaaa aaaacagttt atgcatctgg ttattgactc ttccttttag caaggctatc
    18421 ataaaaatag ttttttgtaa taaaagataa cgaggaagac aatgaaaaaa atcaattatt
    18481 tagcaatcat tttgttagtg ggcctatgtt tgtttgctct gccgacccaa ggattcgctg
    18541 tcgcgggtgg tcatggtgga ggtacatcta gcggtcgaag ttcgggcaga ggttcatttg
    18601 gtggtcgttc atatgggggc agttattttg gcagtcgttc ttacggcaga gattcttatg
    18661 ggtattatgg tccaggtaca ggtccttcta gttatgagca aacgggattt cttgcattag
    18721 ctgttttgtc agtaggaggg gtttctgctt tttctatcgt caagaataaa agacaaaaga
    18781 aaaggctgaa agcggagata ttgtcccgca tgtcaggaac ggctaaagaa aagaaagcac
    18841 agtttctgga aatccaaaag atattcatga agatccaatc cgcttgggaa gaagaaactc
    18901 ctgaaaaagc cagaagctgt tatacagaca agctgtttgc cgaacatcag aaagttctgc
    18961 aaaaaaacaa agcagacggt gtaagaaatc atactaaaaa aataactatt caggaagtag
    19021 gaaattatcg acagatccac gaaaatagtt ttgccatccg aattgatttc agatgctgtg
    19081 attattcagt cgatcgaaaa gatgggcgat tgatctcagg aagtaagaca caacgacagt
    19141 atttttctca gatatggtat tttgattttt ctgagaaaga aaaaagatgg caagccgatt
    19201 ttattcagcc gattatgctg gattgacagg aaatatttat gaacggagtt gtgacaaaag
    19261 ccttgtcgca gctccttttt ctgaataaac ggtgtgccag agatagccta tcggcaataa
    19321 ggcacgattt tgtaaaatat gggaagcgat ttttcaaaat cgcttcttat cgctcaaggc
    19381 tgaacatctc tgtcacaacc tctccactaa atggtgttta aaagcttgca tctgcgataa
    19441 taagaacaac caaacgaaaa ataggaagca ctattttagt ttagttgttc ttattacttg
    19501 atgcaggaca gctttttcac aacctctttt ttgtatagat gggcaaaatt tttgtaataa
    19561 taggcgtatg tctttttcgt tgaggagatg aaaaagcata tggcttattt tttgtttgct
    19621 ttgcttactt tctttccact tcaagtggca gaaatatcta ctttttatta tgtaagcgga
    19681 accatatact tgaaatcaga aggagatcat ttctatgaaa acaagaacaa ttgaaatact
    19741 tcacagaatc atgacagccc aatatccagt aaaagcaaaa catctagcaa gtgagttcaa
    19801 tgtcagtcag cggacgatcc gtcaggaagt tcttgaagcg aatatctggt tgagcagtaa
    19861 aaagcttccg gagatccgta caattcgcaa caaaggtttt ttgttgaagc tggaaaagga
    19921 aaagcaagct cgattagaaa ctgaaataaa agatgtgaaa gacggctttt tgaatcggcg
    19981 cgaacgtatt tttgatctag ttttagccgt tgcttacagc cgaatcccga tccatctaaa
    20041 caaaaaagaa gaagaatttc aaatctctaa aagtacatta gatgaagata tgagacggtt
    20101 acgagcaaca ctggtcagtt atgggataga gatcgtcagt tatggaaagc aaggactggt
    20161 ttataaaggt tcagaacgat ctatccgtac gatgatctat gattttatca atcagaatct
    20221 gggaatgacc gacttcttta tgaatcaaga tgcacaatta acaccggctc aaaaaatctt
    20281 tctccattat tttcctttgg tggaaatcaa aaaaatacag gagatctatt ccaataagtt
    20341 gttgaaacaa gaagatgata tttataaaaa acaaatcctg ctttttacat tgatctggat
    20401 ccagcgaatc aggaaacatg aatttatctc tgccattaat tggaaaaata cagaaataaa
    20461 agacagtagc ttttctgcat ttattgacca catgatcgag gaatttcaat tggacaatgt
    20521 cccgcaagta gaaagaaatt atcttcgttt cactattgag acgttcaatg ctagagatat
    20581 ctccaattca atcgagtggg tacaagctca attactaacg atccaattga tccaatttgt
    20641 agagaacgag acacacgtac ctttccatct aaaggaagaa gctttattcg aaaatctcta
    20701 taagcacatg gctgcactga ttgtaagaat caagaatcat atccaagtca tgaatccatt
    20761 gaaagaaaac atccgaacaa attatttccc aatctaccgc gcagtcactc attttgttcc
    20821 tacgatcgaa gaagtaattg gtggcaagat actggaggac gagatagctt ttcttgtcat
    20881 ccacttttcg acgatcgcca gtgtaatcaa gcgagacttg aattatgttt ataaatcagt
    20941 cgttgtctgt aaccatggga tggctactgg aaatctgtta gcagagaatc tgaaagaaaa
    21001 gtttcctcag atcgagatta ctgcagtttt aagttcaaaa gaaatcgaat tagtagataa
    21061 attggatgtc aatctggttt tcagcacctt tcaattgaat tatcgaaata agccgctttt
    21121 agttgtcgat ccgattttaa ctgagacgaa tatcttggtt atagaagact ttttgaaaag
    21181 aaatgaagaa ctacagcggt tagagcctaa cagtcatgaa tcgacggagt tattcactca
    21241 aataatagat gtcatgaaag aaagtggtgg ccgagtagat cgaatcatct atggcaagtt
    21301 agaaaaatta ttcgaaacaa acaatttaga aatcaacaaa agggagatcc agcctatgtt
    21361 aaaagatatt ttgacggaca gcaatatcat gatcaaagaa aaagccacta cttgggagga
    21421 atcgattgaa ctagctgcta gaccattggt taaagaaaac attgtcgaag agcgctatgt
    21481 cgaagcgatg attcgtgcag tgaatgatta cggaccgtat atcgtgattg gcaaacacat
    21541 ggcactagcc catgcccgac cagaagatgg ggtcaataaa ttaggagtca gcgtagctac
    21601 gattgagcaa ccaatcgatt tcggtaatcc tgaaatggat ccagtaaaga ttattttttg
    21661 tttggcagca gtagactcct actcgcatct gaacatcatg aaagaactaa ttgaattgat
    21721 caacaacgaa gagaagttgg atcgattgat tgaatgcaca aatatcgaag atttcaaaca
    21781 actgcttttt tcagtagttg agaaataata aatgatacgg gggagaacaa agatgaacaa
    21841 attgaacatt ttattcgtat gcggtgcagg tttaggaagt agttttgcag cacagatgtc
    21901 tgctgaggat gttttgaaca aattaggagt agaagccaaa ctggaccatt gtgatatttc
    21961 ttccgcagtt tccatgaaac cagatgtgat catcacagca gaaaattttc gttcgcagtt
    22021 cgaaaagttc gcagttgatc ctaaaacggc gatgatctat ttgaaaaata ttgtttcaaa
    22081 aaatgaaatt gaagaaaaaa tcaccccagt tctgcaagat aagggagtta tctaaataca
    22141 aaaagaaaag agggtaaaaa
//
LOCUS       DAZKLA010000039        22123 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000039 DAZKLA010000000
VERSION     DAZKLA010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.039"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(317..1813)
                     /gene="lysS"
                     /locus_tag="ACG85N_001252"
     CDS             complement(317..1813)
                     /gene="lysS"
                     /locus_tag="ACG85N_001252"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFQ0224036.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1924..2928)
                     /gene="dusB"
                     /locus_tag="ACG85N_001253"
     CDS             complement(1924..2928)
                     /gene="dusB"
                     /locus_tag="ACG85N_001253"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFQ0224037.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2929..3816)
                     /gene="hslO"
                     /locus_tag="ACG85N_001254"
     CDS             complement(2929..3816)
                     /gene="hslO"
                     /locus_tag="ACG85N_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFQ0224038.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4033..6144)
                     /gene="ftsH"
                     /locus_tag="ACG85N_001255"
     CDS             complement(4033..6144)
                     /gene="ftsH"
                     /locus_tag="ACG85N_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFQ0224039.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6234..6779)
                     /gene="hpt"
                     /locus_tag="ACG85N_001256"
     CDS             complement(6234..6779)
                     /gene="hpt"
                     /locus_tag="ACG85N_001256"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFQ0224040.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6779..8224)
                     /gene="tilS"
                     /locus_tag="ACG85N_001257"
     CDS             complement(6779..8224)
                     /gene="tilS"
                     /locus_tag="ACG85N_001257"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFQ0224041.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8344..8814)
                     /locus_tag="ACG85N_001258"
     CDS             complement(8344..8814)
                     /locus_tag="ACG85N_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFQ0224042.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8860..9285)
                     /locus_tag="ACG85N_001259"
     CDS             complement(8860..9285)
                     /locus_tag="ACG85N_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFQ0224043.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9352..9621)
                     /locus_tag="ACG85N_001260"
     CDS             complement(9352..9621)
                     /locus_tag="ACG85N_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFQ0224044.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9632..11227)
                     /locus_tag="ACG85N_001261"
     CDS             complement(9632..11227)
                     /locus_tag="ACG85N_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0224045.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11242..14763)
                     /gene="mfd"
                     /locus_tag="ACG85N_001262"
     CDS             complement(11242..14763)
                     /gene="mfd"
                     /locus_tag="ACG85N_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFQ0224046.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14780..15340)
                     /gene="pth"
                     /locus_tag="ACG85N_001263"
     CDS             complement(14780..15340)
                     /gene="pth"
                     /locus_tag="ACG85N_001263"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFQ0224047.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15693..16667
                     /locus_tag="ACG85N_001264"
     CDS             15693..16667
                     /locus_tag="ACG85N_001264"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0224048.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16837..18237
                     /locus_tag="ACG85N_001265"
     CDS             16837..18237
                     /locus_tag="ACG85N_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFQ0224049.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18315..19037)
                     /locus_tag="ACG85N_001266"
     CDS             complement(18315..19037)
                     /locus_tag="ACG85N_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0224050.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19116..19622)
                     /locus_tag="ACG85N_001267"
     CDS             complement(19116..19622)
                     /locus_tag="ACG85N_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0224051.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19745..21544
                     /locus_tag="ACG85N_001268"
     CDS             19745..21544
                     /locus_tag="ACG85N_001268"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFQ0224052.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21612..>22123)
                     /locus_tag="ACG85N_001269"
     CDS             complement(21612..>22123)
                     /locus_tag="ACG85N_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFQ0224053.1"
                     /translation="TEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVT
                     PAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWT
                     VPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQW
                     FAPDFAVSM"
BASE COUNT         6126 a         4837 c         3999 g         7161 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataacttttt tgttatgttt
       61 ttgaataaat attttcttct gtcttttgga caagtaataa tataccaact aaatttagct
      121 tggtcaacga attttttgta ttttttcaag tttttttcaa taaacgatta ttcataactt
      181 ttctttcttt atcgttcgtt ttcgtcttct ttaatgtcta gaatagtcgg cattagcttt
      241 tgaactttcg ttattattta ttcctttcta cacagcaaaa gctgcctaag gaattcctta
      301 gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga attgattgtg
      361 catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca gttggcggca
      421 taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg tcatttccta
      481 gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga tcgttcagtt
      541 ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga tctgtgaatc
      601 gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt ccgtatacaa
      661 acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg ataacatgtc
      721 cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct aatgcttctt
      781 ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca tctaccatat
      841 ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg atttttgctg
      901 ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc attacatcat
      961 tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta tgtgtagtat
     1021 caattccttc attacggaaa acgcggccga tttcataaac tttttccatt ccgccaacaa
     1081 ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg tccagtgcat
     1141 tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc aatactggtg
     1201 tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg ataatttggc
     1261 ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag tagcgttgtc
     1321 tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga cgaagtgctt
     1381 ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct gttttcatga
     1441 tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact tcataggcct
     1501 catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga tcttgtagat
     1561 gcgcaaatcc agctttccct tttcctcggc gagtcatcat acgaccagct acactagcgg
     1621 tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca tgcaattctt
     1681 ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct tcacgcaatt
     1741 gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact tgatgttgtt
     1801 gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat aatgccaatg
     1861 aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg aaaaacttag
     1921 aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt gatccagcag
     1981 gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt tcgctgctcg
     2041 aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag ctactttttc
     2101 tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt tttcggctgg
     2161 tgttggttca ggaatcagtt cccctgtttc gagataatgc tgtgtgcggt gaatcatcca
     2221 tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat agtcaagcat
     2281 ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggaa tcgtcaaatg
     2341 ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct gaacccgtgt
     2401 acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg cattttctac
     2461 agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg ggatatttac
     2521 tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa gccattttgc
     2581 tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat ctattatatc
     2641 ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt tatttccgcc
     2701 aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct ctaaagtttt
     2761 tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta agcccgcacc
     2821 aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg gtgcaacaac
     2881 gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt aaatccctcc
     2941 taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt tgttattaca
     3001 gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga tttcttctgc
     3061 acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt tgaattgaac
     3121 aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca atatttcttc
     3181 tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta attgttcttc
     3241 gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga atcctcccgc
     3301 cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg aagggatttg
     3361 ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa caatcggcgt
     3421 ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca ttccttgtgt
     3481 tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac tgagatgcgg
     3541 atttttgatg taacctttca catctccctt tccatttgca tctactacaa ttcctgttgc
     3601 tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg tcgcaccaag
     3661 tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc aagtatcatg
     3721 acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc ggacgaatcc
     3781 gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc tttttccgtt
     3841 tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg gaatgcttct
     3901 tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac aacttaaata
     3961 aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc agatcaattg
     4021 acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt gttgagaatc
     4081 tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt caaaatcacg
     4141 tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg cttcttcaaa
     4201 agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag gcatcacacc
     4261 gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca aaagtttttc
     4321 agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt tatcatgagc
     4381 ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt ccgagtaagc
     4441 ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat attgaacagg
     4501 acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag ctgtcgcttg
     4561 ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt cagctgtacg
     4621 tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca agaactgatc
     4681 ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga tcgttacttt
     4741 gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag cttcgtgata
     4801 agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg gacctgcaat
     4861 cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat ttcttctagc
     4921 agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa aacctggtgt
     4981 ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc gtgcatggac
     5041 tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct ggcggtcaaa
     5101 acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg cagcaatgac
     5161 gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt tcaatgtttg
     5221 ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta ccgcatcaat
     5281 ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca aatcacgaac
     5341 acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga tcgagtaaaa
     5401 cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg ttcctggagg
     5461 tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc ggcgaggatc
     5521 tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac ccgctacatc
     5581 agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt taccaaagtt
     5641 catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca tgtaaaagaa
     5701 aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata taccgctgct
     5761 tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg ttacttggct
     5821 caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg tatctgttga
     5881 acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt attcaccagt
     5941 gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc ctgcttgtag
     6001 ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat taccaaaaat
     6061 gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg catttttcat
     6121 catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta atttaacagt
     6181 tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca gcattaattt
     6241 gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata tgcttctgca
     6301 taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac ataatctgct
     6361 tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt gactgatttt
     6421 gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct gtcaatgata
     6481 tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt gacgattttt
     6541 acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa atccaattcc
     6601 aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc ttttaagaca
     6661 cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc tagttctttg
     6721 caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt ttccaacatc
     6781 aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag tgtattttat
     6841 cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta tatatatttc
     6901 ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact ttctgatcga
     6961 taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg tctcctgctt
     7021 ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga gaaaatagag
     7081 accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga ccaatccatt
     7141 ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg tcttctgtct
     7201 ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct cttttcagta
     7261 tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac tggtctaaaa
     7321 tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct ttgtcgaaaa
     7381 aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt tctgcagaaa
     7441 atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc atatattttt
     7501 gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt ctcaacgcct
     7561 gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt ctctggatat
     7621 ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct gcataagtat
     7681 ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta gcaaatggtt
     7741 gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt ttcattaaca
     7801 ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat tgtccttctt
     7861 ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct atggcggatt
     7921 tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca caatatgagc
     7981 gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg tggacgatac
     8041 ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa accatcgaat
     8101 caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat ccttgttcat
     8161 tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct ttttcaacgt
     8221 tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt ctaaaacgac
     8281 aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat tatttgcggc
     8341 tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc gcttcaatga
     8401 agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga agtcttcttt
     8461 ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat ttcggttgtc
     8521 acggggatct ttttgttgat attttttagg acggctttct tctccagatt cagcaggttg
     8581 ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg aaataacttt
     8641 aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat agccatttga
     8701 cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa acgcaccaaa
     8761 atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg acataaaaaa
     8821 aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag ttgtagaact
     8881 agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata taggataaac
     8941 tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta catactcatc
     9001 atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga cttctttcat
     9061 ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca tgtcatggaa
     9121 aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg cagccagtct
     9181 gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact ttttctttgc
     9241 atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga ttcgctccaa
     9301 tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa tctatttctc
     9361 tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat cttctttttt
     9421 ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa actggacttt
     9481 caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt tgatttgaat
     9541 tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc gggaaatttt
     9601 taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta tttaatcgca
     9661 ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga cgaagacgaa
     9721 tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg acaaaaaaag
     9781 cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa tacagcagca
     9841 gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc cgttctttcc
     9901 aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc acacctaatc
     9961 ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga aaagaagtag
    10021 tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga aacgagttct
    10081 tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg gaaaaaacaa
    10141 ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg atataaggga
    10201 gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat ctaagataga
    10261 ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga gtcaacgcag
    10321 gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact aaaggctgac
    10381 ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg taaacttcca
    10441 aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat ccactataaa
    10501 tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac ggctgtttga
    10561 acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca tagtagtgaa
    10621 ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc gtccctgttt
    10681 ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc acaaaaacac
    10741 gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat cttccttgga
    10801 aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa actacttgga
    10861 tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta aatgcaaaga
    10921 caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt tgcaaagctt
    10981 gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac tgcggaagac
    11041 cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga taaacataaa
    11101 aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt aatacttttg
    11161 ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc atttctttat
    11221 gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca acgctttgac
    11281 aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga tcgtcaagcg
    11341 tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg ctgtcagtgc
    11401 ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag taaagactat
    11461 atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca ttttgatctg
    11521 cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt cgagcagatc
    11581 atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt tatagatctc
    11641 gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga caccgaggtc
    11701 gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag aaacagcttc
    11761 gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat gctgctgagc
    11821 acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct tgaatccaga
    11881 tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt cgttaaggat
    11941 cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc ctacccgccc
    12001 tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga acaacgtatt
    12061 tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa catcatattg
    12121 accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt gcccatgagc
    12181 ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt gttcgatcgt
    12241 ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc gatggattgc
    12301 ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat aacgattcgc
    12361 aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat gcaaagttct
    12421 cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact gtttcaatcg
    12481 ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac ccaaatcgct
    12541 aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa cttgacctgt
    12601 acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac ttaatagccc
    12661 gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct gctgtgccaa
    12721 gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt taaaggccgc
    12781 tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc gatccattgg
    12841 tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt catcagtttc
    12901 agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc caaaagcata
    12961 tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga tatcctcgat
    13021 ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct tattgatgcg
    13081 aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag taacaggaat
    13141 aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa cgccatctac
    13201 ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga caacgtaatc
    13261 tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg tttgacgtct
    13321 cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta ctattttttc
    13381 ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct gtcctctgat
    13441 caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca tttgttctgc
    13501 tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt gcttgcgcca
    13561 acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca tcgtccgata
    13621 ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga agagtgagaa
    13681 aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg aaaatgtctg
    13741 ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg cttccctttc
    13801 gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta aactatttga
    13861 agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt agaattttgc
    13921 ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt aatcttgcaa
    13981 aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca ttttttgcat
    14041 ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg ttggggaaac
    14101 ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat accgcataga
    14161 atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta acgggtaaat
    14221 atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct cttctcgtac
    14281 atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat caccaatatg
    14341 ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt gcaaagatgc
    14401 gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg ttgcaactct
    14461 ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt catccactgg
    14521 aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta actgattcgc
    14581 ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa acgctcgagc
    14641 aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag gcattttttc
    14701 ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt gaataatatc
    14761 cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac gaatgccctt
    14821 ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc aaaggctgat
    14881 tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct tctggcctgc
    14941 cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata atacttttga
    15001 taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc actgccagat
    15061 ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa gtcattagtg
    15121 ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa aggatctttt
    15181 ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg aaagtagctt
    15241 gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt gtatattggt
    15301 atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc cacctaattc
    15361 taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg agcttttttc
    15421 ttttttacac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc taaaagagaa
    15481 tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa taattgatat
    15541 ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc ttgttatctg
    15601 ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca taagcatggg
    15661 aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa aaaaaagatc
    15721 atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc tttgctctag
    15781 ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct aaaactgaag
    15841 gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa atctattctg
    15901 ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc gcaccacaaa
    15961 aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac cgcgatgttg
    16021 tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct aacccagtag
    16081 atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc gtaatcggtt
    16141 caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta gttgacgttg
    16201 atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa ttcccagttt
    16261 ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac aacccagaaa
    16321 tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct tacacaatta
    16381 tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt atcactcgtg
    16441 ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat ggcgaatatg
    16501 gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt atccaaaaag
    16561 taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct gctaagcaat
    16621 taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct ttgaacatga
    16681 cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat ggtttctaaa
    16741 ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta tcatgagacc
    16801 gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc gcatcgaaaa
    16861 aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct gatcagcttt
    16921 tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga aataaatgga
    16981 ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga agcttattct
    17041 gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc aaaatccgag
    17101 ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga acaaaacgga
    17161 tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga tcctgtcagt
    17221 caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct gactgatttg
    17281 aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga ctctttcgtt
    17341 atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa acaattaaaa
    17401 agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga aaaaccaaaa
    17461 gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc tatcgcaaat
    17521 gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag tgatattatg
    17581 agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt acgtcaatat
    17641 gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt taaaagtaca
    17701 aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca aacagacagt
    17761 gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg ttcacccatt
    17821 gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat cgaagttgat
    17881 ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga aacagatatc
    17941 gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg gaataaagaa
    18001 gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag tccggtaaac
    18061 tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg gcaacctgaa
    18121 tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac cccaccaagt
    18181 gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt cttctaacaa
    18241 aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct ttttattatc
    18301 taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc atcatcgtct
    18361 tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa atcaatcgtc
    18421 gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc caactcatct
    18481 cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg agaagtttgc
    18541 agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc aacagtcaac
    18601 ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac tgaacccgtt
    18661 tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc tgtccgatta
    18721 cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata tccttcatac
    18781 gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat cgcccgttcc
    18841 acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg caaagctgga
    18901 ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg agaaagtttc
    18961 tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat attatgccat
    19021 ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac ttctattcta
    19081 gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt gaatgccgca
    19141 aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat gacatcttgg
    19201 aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc tgcgtaccct
    19261 gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt ggggaaaaca
    19321 cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc cttccttaaa
    19381 aagaactcta aaaatttggc gtagcccaga tggttgatac tcaccatact tccaccataa
    19441 ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca gctttctaat
    19501 cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa gattataacg
    19561 actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt tgaagaattc
    19621 ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat cattttcata
    19681 gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg aagggagttt
    19741 tttcatgagt tattcattaa attggaatct agattctatt ttttccggag gcagtcattc
    19801 tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt attatcaccg
    19861 cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta ttcttcaatt
    19921 gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg cattgctttc
    19981 tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg caatgctgcc
    20041 tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat ctgacaatga
    20101 ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt taaacgagat
    20161 tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa atacgctttc
    20221 tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca gcatttcgat
    20281 tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct tcaataagat
    20341 gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg aagaagcctg
    20401 gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt ttagactttc
    20461 tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt acaatcggtt
    20521 gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac aaccccttgt
    20581 taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat ggcaagatca
    20641 agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg acgaagcagc
    20701 agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat ttgcccaatc
    20761 tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc ctggtggtta
    20821 ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca gtaattctgt
    20881 taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca gcacgatgtg
    20941 ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag caagtacatt
    21001 cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag aagaaaaaat
    21061 caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata tccattcccg
    21121 attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct cagaagataa
    21181 gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt tagctactta
    21241 ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac cgttttataa
    21301 cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg ccaaccaaaa
    21361 gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct caatgacgac
    21421 ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt tttggcaagc
    21481 aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag aagaatttat
    21541 ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta actctttttg
    21601 attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac tgaaccaact
    21661 tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa agcaatcgct
    21721 acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt tgcttgagaa
    21781 actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat gtcacgacca
    21841 gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact tggatcttta
    21901 ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat tgctgcacca
    21961 ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc aggtgttggt
    22021 gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac actttgatct
    22081 gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttg
//
LOCUS       DAZKLA010000040        21972 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000040 DAZKLA010000000
VERSION     DAZKLA010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.040"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..1167
                     /locus_tag="ACG85N_001270"
     CDS             28..1167
                     /locus_tag="ACG85N_001270"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFQ0224054.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            1160..1960
                     /locus_tag="ACG85N_001271"
     CDS             1160..1960
                     /locus_tag="ACG85N_001271"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0224055.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            1960..2787
                     /locus_tag="ACG85N_001272"
     CDS             1960..2787
                     /locus_tag="ACG85N_001272"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0224056.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            2792..3499
                     /locus_tag="ACG85N_001273"
     CDS             2792..3499
                     /locus_tag="ACG85N_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0224057.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            3599..4465
                     /gene="rfbA"
                     /locus_tag="ACG85N_001274"
     CDS             3599..4465
                     /gene="rfbA"
                     /locus_tag="ACG85N_001274"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFQ0224058.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            4479..5051
                     /gene="rfbC"
                     /locus_tag="ACG85N_001275"
     CDS             4479..5051
                     /gene="rfbC"
                     /locus_tag="ACG85N_001275"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFQ0224059.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            5073..6101
                     /gene="rfbB"
                     /locus_tag="ACG85N_001276"
     CDS             5073..6101
                     /gene="rfbB"
                     /locus_tag="ACG85N_001276"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFQ0224060.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            6199..7050
                     /gene="rfbD"
                     /locus_tag="ACG85N_001277"
     CDS             6199..7050
                     /gene="rfbD"
                     /locus_tag="ACG85N_001277"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFQ0224061.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            7084..9117
                     /locus_tag="ACG85N_001278"
     CDS             7084..9117
                     /locus_tag="ACG85N_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224062.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            9161..10441
                     /locus_tag="ACG85N_001279"
     CDS             9161..10441
                     /locus_tag="ACG85N_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFQ0224063.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            10651..11457
                     /locus_tag="ACG85N_001280"
     CDS             10651..11457
                     /locus_tag="ACG85N_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0224064.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            11469..12689
                     /locus_tag="ACG85N_001281"
     CDS             11469..12689
                     /locus_tag="ACG85N_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224065.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            12679..14265
                     /locus_tag="ACG85N_001282"
     CDS             12679..14265
                     /locus_tag="ACG85N_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0224066.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            14304..16442
                     /locus_tag="ACG85N_001283"
     CDS             14304..16442
                     /locus_tag="ACG85N_001283"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0224067.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(16810..17793)
                     /locus_tag="ACG85N_001284"
     CDS             complement(16810..17793)
                     /locus_tag="ACG85N_001284"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0224068.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            18171..19562
                     /locus_tag="ACG85N_001285"
     CDS             18171..19562
                     /locus_tag="ACG85N_001285"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0224069.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            19578..20618
                     /locus_tag="ACG85N_001286"
     CDS             19578..20618
                     /locus_tag="ACG85N_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0224070.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            20637..21722
                     /locus_tag="ACG85N_001287"
     CDS             20637..21722
                     /locus_tag="ACG85N_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFQ0224071.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            21755..>21972
                     /locus_tag="ACG85N_001288"
     CDS             21755..>21972
                     /locus_tag="ACG85N_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HFQ0224072.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQA"
BASE COUNT         7115 a         3575 c         4496 g         6786 t
ORIGIN      
        1 tgactcttaa ttaaagtagg tgcccatatg cttgtttctt ttagtatggt tataagattt
       61 tttctgacaa ttttattatc actgatatta acacctgtat tcaaaatcat ttcagtacaa
      121 acaggtatgg tcgacaaacc aaatgaaaga agaataaata aagttccaat gccttcagcg
      181 ggcggactgc ctatctttat ttcatttgtt atatcgactt tattcttatt ccgtaaaatt
      241 atccctcaat cgtatattgt accgatcttg attgcttctt tagtaatcat agtcacagga
      301 gtgctcgatg ataaatatga gctgacacct aaacaaaaaa gtatagggat cttgattgct
      361 tctcttatta tttattttgt agcagatata cgtattgatt ccttcacgct tccttttatt
      421 ggatatatcc aactaggctg gcttagtttt cccgtaacga ttttttggat cttcggtatc
      481 acgaatgcgg tcaatctgat tgacggtttg gatgggctgg cggcagggat ttcattgatc
      541 ggtttaatta cgataggaat cattggatat tttttcctcc atgcttctac agtgtatatt
      601 ccagttgtta ttttctgtct tgtagcaagt attatcggtt tttttcctta caatttttat
      661 ccggcaaaga tttatttagg cgatacagga gctctattcc tagggtttat gatggccgtc
      721 ctttcactac aagggctgaa gaacgtgact tttgtttcat cgatttcttt gctgatcgtc
      781 atgggagtgc cggtaacaga cacgtttttt gctatcatta ggagaaaagc aaaccgtgtt
      841 tctttttcta cagcagataa aaaacatctt catcaccgac taatcagttt aggctttact
      901 cataaaggtg ctgtcctgac gatttatgcg atggctttga tgttttcttt cacagctatg
      961 gtcatgaatt atactggaag aatcggaacg atcatattga ttattgccat gttgtttgct
     1021 gcaattttat tatttgaatt gattggtctg atcaatgaga aacatcagtt tattttaaaa
     1081 acattgcgat ttttagggaa taaagagtac cgtacgcaag taaaacagaa atatttgaag
     1141 aaatggaacc gtcatataga tgaataacaa attaaaccag ccgatcctta ttagtattgt
     1201 gacggcaaac agtgataaaa tttttcggac actagataat tttatttcaa ctgtaaaaga
     1261 tgaaacagca gtaaaaatcc gaatttttga caacaattca cttcccgagt acaaaaaaag
     1321 actaacagag tataggaaat atccctacat cgatatctat tttcatgatg aaaattccgg
     1381 atttggttat ggacataatc acaatctgat agctagcgat tctcgctatg gcattatttg
     1441 taacccagat attttagtgg atgaagagac agttacagca ttggtcttgt tattgaaaga
     1501 acatccggaa tgtgcgatgt tagcacctaa gattcttaat gaagatggaa caactcagca
     1561 cctcatccgt gagcagctgg ctgtttttga ttacgttttg cgctttgtgc ctttcaaatg
     1621 ggtcaaaaaa aagtttgaca aacgactggc tgtgtttgaa tgccgaggat tgcctactga
     1681 tgctaatagt tatgtacgga tgatctcagg tagttttatg gtagctgata ttgataaatt
     1741 taaagagata aaggggtttg atgagcgttt cttcatgtac tttgaagaca acgacttgtg
     1801 tttgagtttt gaaaaagcag gttacaagtt attgtacaca cccttttatg cagttatcca
     1861 tttttacggg aagggtgcgc atcgaaatgc aaaactgttt cgcgtgtttt tgacttctat
     1921 gatgaaattt ttcaataagt gggggtggcg ttttttctga tgaaaccaga aatattagta
     1981 gtcgttgtgc tttatcagca actattcagt caatctccag cttatggacc attaagcaaa
     2041 gcacttagcg aaaagcaaat tcagcttatt atttacgata atagtcctca aaggcagcaa
     2101 aatgagctgt ttgaaaaaga aaatatcgtt tatcatcatg atccgcaaaa tcctggttta
     2161 gcggtggctt ataattttgc gttgtctcaa gcagatgaag gaacacgttg tctcgtcact
     2221 ctggatcagg atacgaggct agtcgacgat tattttgaac tattgcgtaa agtaaccttt
     2281 acagatgaat gtgttgctgc agttccgatg attttttctg ggagccgaca agtttctccc
     2341 ctatattcag atcgttatat caatcgtaca gctcaagcag tagaagttga aggcccaaca
     2401 gctgaacgaa tcatggcaat caattcaggt gttgcctggt ctgtcaaatt tctaaaaaag
     2461 attggcgggt ttaatcctgc tttttcttta gattttttgg atcattggct gttttggaaa
     2521 gtggatcagt tggaaaaaac tgtggagatc cttcctgtac gaatggaaca tgatctttcg
     2581 gttttagact atgaaaaagt ttcaagtaat cgataccgtt ctattttaca agcagaaaat
     2641 cgattttatc atgaatatga tcaaaggcat ttatccagtc accgacgaca attattgtta
     2701 agaacagcta aacagttctt gaccgtcaaa aaccggcata tctggcggat gactttgcgc
     2761 tcatatgtta agaattggaa ggtgtgaatt cgtgcgttcg gtttgtatag ctacctttaa
     2821 tggaggcaga tatttaagag agcagttaga tagtatttta tctcagcttt cagcagaaga
     2881 tgaagtagtc atttctgatg atgggtcagt agatgataca tgggatatat taactgaata
     2941 cgcagaaaaa gataagcgaa tccagctttt ttcggggcct cgtcaaggat tgatcgctaa
     3001 ttttggttat gcgatccagc agacgaaagg agatctaatc tttttggcag accaagatga
     3061 tgtttggttg gagacaaaag tgaaaaagat cactgactat tttgagcaga atccggatca
     3121 gctagtagta gtttctgatt taatcattgt ggacagcgag ctgcaagaga tccatgattc
     3181 gtatttttcg tttcgaaatt ccaaagaagg atggcttaat aatgtgataa gaagtcacta
     3241 tattggtgct ggtatggctt tccggtcttc tttaaaaccc gatattttgc cgatcccgtc
     3301 agaagtgccg atgcatgata tgtggattgg tttgctggga ggaaagaaga ctgggttcct
     3361 aagagaaccg ttaactctgt accgaagaca tgatttaaac gcgagtgaga ttgaaacaca
     3421 ttctaaatta cggcaaaaaa tttcttggcg tgttcatctg attacggcgt tgatcaaacg
     3481 aaaatggttt aaacgttgat aaaagatgtt taaatcctta atatttccat gacaaatata
     3541 acaattgatg agataataac aaagtgaaat ttgaaatcaa aaaggagagc agatatccat
     3601 gaagggaatc attcttgctg ggggcagcgg aacacgtctg taccctttaa caaaggcaac
     3661 atcgaaacaa ttgatgccga tttatgacaa accaatgatt tattatccaa tgtctactct
     3721 gatgttggcg ggaatcaaag aaattctgat tatttcaacg ccacaagata caccacgttt
     3781 caaggaatta tttggaaacg gaaatgattt aggactgcag atcgaatatg cagtacaaga
     3841 aagtccagat ggattggcac aagcgtttat cattggagaa gaatttattg gagatgatag
     3901 tgtctgcttg gtattagggg acaatattta ctatggtggc ggtctgtcta aaatgttgca
     3961 acgagcagct tcaaaagata caggcgcgac agtatttggc tatcatgtca atgatccaga
     4021 acgctttggt gtagtagaat ttgatgagaa tatgcaagct ttgtcgattg aagaaaagcc
     4081 tgctcagcca aaatcaaatt acgctgtaac tggcctttat ttttatgaca atgaggtaat
     4141 ttctatcgct aaaggtatca aaccttcaga acgtggagaa ctagagatta cggatgtcaa
     4201 taaagcatat ttggataaag gaaaactatc cgtagaactt atgggacgcg gatttgcttg
     4261 gcttgataca ggaacacatg aatcattgct tgaagcatct acctttatcg aaacaattga
     4321 aaaacgtcag aacttaaaag tggcttgttt ggaagaaatt gcttaccgta tgggctacat
     4381 cgataaggaa caattgctgg cccttgcaca gccattgaaa aaaaatcaat acggtcaata
     4441 tttattgaat ttggccgctg aataaggagt ccttacagat gaaagtaaca gatacaaaat
     4501 taacagatgt caaaatcgta gaaatggacg tctttggcga tcatcgcggt ttcttcacag
     4561 aaagttattc aaaagcaaaa tttgcagaac atggattgaa atatgacttt gttcaagaca
     4621 accattcact atcagctgaa gctggtgtcc ttcgggggct tcatttccaa aaaggagaag
     4681 ctgcacagac aaaattgatt cgggtagtca ctggagcagt tttagatgta gtagtggata
     4741 tccgcaaagg tagcccgact tatggtcaat gggacggatt tatcttatct gaacataatc
     4801 atcgacagtt gcttgtacct aaaggatatg cccatggatt cgttactttg acaccaaatg
     4861 tgaacttcct ttataagtgt gataattact ataatgcaga agcagatggg ggcatcgcat
     4921 ttgatgatcc agatttagcg attgattggc caattgatcc atcaagagcg attatgtcgg
     4981 aaaaagatca ccgtcatccg acattaaaag aatttgaagc agaaaatcca tttgtttacg
     5041 gagaaatcta agaattaagg agaaattttc ttatgaaaaa catcattgtt accggaggtg
     5101 ccggattcat cggctctaat ttcgttcatt acgtggtaaa caatcatcca gaagtgcatg
     5161 tcactgtttt ggataaactg acttatgcag gaaacaaaga gaacttggct ggacttccta
     5221 gcgatcgcgt agaattggtc gtaggagatg ttgcagatgc agaattagtg gaccgtctag
     5281 taaaagaagc ggacgcagtt gttcattacg cggcagaatc ccataacgat aattcattaa
     5341 aagatccttt cccatttgta caaacgaata tcattggtac gtatacactt ttagaagctt
     5401 gccgcaagta tgatgtacgc tatcatcacg tatctactga cgaggtttac ggcgatcttc
     5461 cattacgtga agacctgcca ggacatgggg aaggcgaagg agaaaaattc actgcggaaa
     5521 caccatataa tccttcaagt ccatattctt ctacaaaagc tggttcagat ctgttagtaa
     5581 aagcatgggt gcgttctttt ggcttacgtg caaccatttc aaactgttca aacaactatg
     5641 gtccttatca gcatattgaa aaatttattc ctcgccaagt aacgaatatc ttaagcggta
     5701 ttcgtccaaa attatatggc gaaggaaaga atgttcgtga ctggatccat acgaatgatc
     5761 attcttcagc agtgtggctg attttgacga aaggcagaat tggcgaaacc tatctgatcg
     5821 gtgcagacgg tgaagaagat aataagaccg tgatggaatt gatcttagaa atgatgggtc
     5881 aaccaaaaga tgcgtacgac catgtaaacg accgtgcagg acacgacttg cgttatgcaa
     5941 tcgattcgac aaaattacgg gaagagttag gatggaagcc tgagtttacg aacttccgtg
     6001 atgggttggc tgacacgatc aaatggtacg aagaacatga agactggtgg aaaaaagaaa
     6061 aagaggccgt tgaagcagca tatgccaaaa atggacaata atacagagaa aatgtcactg
     6121 tattaatgga aaaacacatg tgaggcaggc gctcgcctgt ctctttttta aaataaaaat
     6181 caaggagcgg ataatcatat gtttttatta actggaggaa atgggcaact aggtacagaa
     6241 ttgcgccatt tattagatga aaaaaatctg caatatgttt caacagatgc aaaagacttg
     6301 gatatcacag atgccgagaa aacaatggct tacatcacag aattaaaacc tgatgttatt
     6361 ttccattgtg cagcttacac agcagtagat aaagcagaag aagaagcaaa agaattagat
     6421 gagaagatca atgttgatgg tacaagaaat gtagcacttg cagcaaaagc tgtgaatgct
     6481 caattcgtct atatcagtac agactatgtt ttcgatggaa aaaacaaaaa tgtagaatac
     6541 gaagtagacg accaaacgaa tccactaaat gaatatggtc gtacgaaatt attaggcgaa
     6601 aaagcagtag aagagatttt agaagactat tatatcatac gaacttcttg ggttttcggg
     6661 atctatggac acaatttcgt cttcacgatg caacgcctag cggaaacccg cgacaaactg
     6721 actgtagtaa atgaccagtt tgggcgacca acttggacac gtactttggc agaatttatg
     6781 gtatatgtca tcgaagaaaa agcaccattt ggtatttatc atttgtcgaa cgaaaatagc
     6841 tgttcttggt atgaattcgc aaaagaaata ttgaaagata cagatgtaga agttgcacct
     6901 gttacgtcag aagaatttcc gcaaaaagca acacgtccgc agtattcagt catgagcctg
     6961 aaaaagacag aagagttagg atttgtcata ccaacttggc aagaagcact tgcgcaaatg
     7021 ctggaagctg ccaaaaaaat gaataagtaa tttcctttta tcaaatagaa tttggagcat
     7081 gatatggaaa aacaaacaag aagtgcacga cgaaaagaaa agccaaaagc gaaccaggca
     7141 gattcgaatt tggtgcttga cctgtttcta aaactccgct atcttattgg cgttttagtc
     7201 atcgtggtca tcgttacttt taatttaaac ggaagttcac ttggtgtttg ggataaatat
     7261 gtgtcgcaaa gagacgatgg gaaaaaaact gatgtgattt ttggacagaa tcgggaagtg
     7321 cggtcagacg aatggctggt gcagacacct ttttacctct cacaagctga agaggacttt
     7381 ccgttggtga acgaggatta ttcattaaac ggacaaaaca tgatcattgc gtataattct
     7441 cctgtaaagg atattacggt tatcggaaaa ccattcaact ggggattctt tttcctcgga
     7501 agagatcgag gactttcctt ttattgggcg atgaaattag tcgtcatggt cttgcttgga
     7561 ttcgaagtac tgatgatatt aacgaagaga aaaaaagctc tatcgctaat cggtgcattt
     7621 gcgctgacat tttctccagc agtccagtgg tggttcatgc aacatgtggg cgatttgatt
     7681 ttctttactt tgggactgat gactgctttt tatcattatt tctacaatca tgataaaaaa
     7741 tggctacgaa cgttgatgat gcttttggtg gttattttcg gtattgggtt tattttagtg
     7801 atttacccag cccatcaagt gatgctggcg tacttgctga ttttttattt tgttggcctg
     7861 cttatttact atagcaagaa agtttcatgg gatctgttgg atgtagtgtt gattggaggg
     7921 gcagttttat ttattggcgg agtcatggtt catttctggc taacgtcaaa agacgcattg
     7981 ttagcttctt tgaatacctt atatccagga aaacgggtaa gtactggagg aaagtggacg
     8041 attggcgaat ttttttcttt ccttacaaat tggaaaattc cttttgaaga tatcaactat
     8101 tcgaataact ctgaagtggc cctgttctat cacttttttc caactgtatt cctagcaagt
     8161 ccatttgtgt tatttggcaa aaaagatagc gaacaaaagt tactgggacg tattttgatg
     8221 attttttgtc tgttttctat cttttggatt accgttgggt tgccaaaatg gctagcagaa
     8281 atcactctgc tgtcttatgt tcctccatta agagccatcc ttacttttag ttttgcggct
     8341 tgtttattat cgatctggtt tatcgcttat atttggagag aagatgttat tcctatttgg
     8401 tataaattca ttttgttggc agtaaatgca tggtcttatt tctatgcgat tacgcattcg
     8461 aaaatggata attatttttc ggattttgaa accatcgcag ttgctgtgtt gggaacagtg
     8521 attttagctt ttgcattatt tggctggaga cgcttgtttt acctcgcttt tgccgcctta
     8581 gttatcgtat ctggcttttt cgtcaatcca gtcgtagaag gtacaggagc gatttttgaa
     8641 aaaacactgg caaaagaaat ccaaaaaatc gatcaacaag atcctggtaa agtttggttg
     8701 tctgaagatg aattatataa cttcacccca acattgggag tgaaatcctt caatacagta
     8761 cgcttttatc cagatatagc tgcatggaag aaaatcgatc cgaaagaaga atacgagaag
     8821 tattataatc ggtatgctca tacaagagct tttatcgcta aggaagatac aaagttcatt
     8881 ttagatcgtc cagatatgtt ttcggtcacg ttgaattttg aagacgcaga aaaattaggg
     8941 atcgactatg tgatcagtaa acgaccatta actgattaca accaattata tgaagcgcag
     9001 ttcacgcagc tatatggacc ggataaagac ggttatatga tttttaaagt agaatatccg
     9061 gattatccag atgtaccaca aacacaaact caggaaaatc agctagtcgt acagtaatag
     9121 gctagctgag aataatcctg aaatgttagt agaaaggaga gtggagataa ttcaagcaat
     9181 cgtacaaaaa tttgtcaaca aaatgagtgt cggtttcttg cttttgttac tgacgctagt
     9241 cttttttgtt tggacagcag ggttaggttt tccagcaact ttgtttatat ataacaatag
     9301 ttcattactt atcgctttta gtgtggctat tttgctcatt ttaaatgttt ataaagcaaa
     9361 aggcgtcgat tttctttatc taggtgttgc gcttgccctt ttcattttct tttcttactt
     9421 tcgggaatta agaaatgagt ctttatttgg agacttactg tttcctttga ttattttatt
     9481 ttgtttagta attaaatgga tcgagtggac aaaatgggat cgcggtattt atctgattgt
     9541 cttttcggtc ttcctcggaa tcacgttata tcgtgtattt acagagatcc aagtaccaga
     9601 aggacaaagc atctgggctc caggcaattc gctttctgat atttggatca atacgaatat
     9661 aatcggaagt tctttgatga ctcttgcttt attgatcagt ggatttgcca gttcttttga
     9721 acgatggtat gtacgaatca tcggtgcagt agcagttgtt gcagcttttc tcgggatttg
     9781 ggtctgccaa tctagaggtg cattagtcgc gttgattgtt tttgcgatat tggatctttt
     9841 acctaaatcg ttgatgcgtg tcattcgtgc gccatttatt gcttacacgg ttacgattct
     9901 tttagctttg ccgatttctt atctggcggc tgtctctgaa aaagtcaatt tgtttaccgg
     9961 tcgagaagat atttggcata aattctatca gacgctagga gaaaaatctg aacaaatttt
    10021 actaggaatg aaaacattta tcttccaacg aggaaaccaa tttttaggaa accacaatag
    10081 ttataattct attttgaata tctatggatt gataggattt gggattgctg ctttgctgct
    10141 tatccttttt attggtcgct taacgttgaa agcagattta tccaacgggc agatgacttt
    10201 tatctgggcc ttttttgcgg tgatgatgca gtcatttatg gaggatacat tgacttcata
    10261 cccatgggtt ccgattattt atatcctgtt ggctatggcc gttcatcgat atgatgaacc
    10321 agaagttgcg gaggaattct atgttatgga agaggcatat gacgatatga cttatgatga
    10381 tacatcttat gaggaacaac cagatagcgc tcattcttcc aggatccaac gctatcattg
    10441 ataggcacaa aatacgagag aggctgtgac aaccgtcgca gccttttttg gtttattgca
    10501 attgtctagc atcttctgta aaatagaaac gttagagaaa atcttgtatc atagaaaatt
    10561 aagaaagaaa taagaacgca gctcggtacc aaaaatatgg taaaatggca cttgactgta
    10621 attcactgaa aatcgcttgg aggaacacaa atgtttaaag atttgtattt atttatgaag
    10681 gacatttatc agaacagaaa attattgcta cagttttcga tcaatgactt caaatcaaga
    10741 tatgcaggat cgtttttagg aatcttatgg gcgtttatca atccgctatt tactgtattg
    10801 atttattggc tggtatttgg tgttggactg aaagcacaaa tgatcgatgc aaaataccca
    10861 tttatcgtat atttagttac cgggatgatt ccctggttct tcttctcaga tgcatttgca
    10921 tcaactacgg ttgtttttcg ggaatatact tatttagtaa agaaagtcgt attcaatatt
    10981 cgtatcttgc ccacgacgaa aatcctatcc aatctgtaca cccacttatt cttcattctg
    11041 attggcatag ctgtcgcgct tataaatggt atatatcctt cagcaatgtc tttacagctg
    11101 atttattatt tattttgcat gtgtgctttc ttgacaggat tgacttggct gacggcatcg
    11161 atccaaccat ttttacctga tatcatgcaa ttaattacga tcgtgctgca gttgatcatg
    11221 tggacattac cgatattatg gagccctaat cagtttgatt caggtcttgt aagcatattg
    11281 aaattaaatc cgctgtacta tgttgttcaa ggttatcgtg agtcattttt aagtgaagct
    11341 tggttctggg agcatggaac atatactttg tatttctggg ttttcacgtt agtaatgttc
    11401 gtaatcggct ctgcagtgtt ccgtagattg aaaccacatt tttcagatgt gctgtaagga
    11461 gaatagctat gacagaatat gcaattagac taaaagatat cacaaaatct tataatatgt
    11521 acgcaaaacc ttcggatcga tttcgtgaag ccctcaatcc ttttaagaaa tcctatcatg
    11581 atgtatttta tgcgttgaaa aacgtgaatg tagaaataaa aaaaggtgag atgatcggtt
    11641 tcgtgggaga aaacggatcg ggaaaatcta caatgttgaa aatcattacg ggagtactga
    11701 caccaacatc cggcacgatg gaaatcgaag gaaaaatctc agctttgcta gaactaggat
    11761 caggattcaa tcctgagtat tctggctatg aaaatatcta tttaaatgga atggttctag
    11821 gattttcaag agaagaagtc gatgaaatgg tcgatgatat cattgaattt gctgacatcg
    11881 gagaccatat ttatcaacct gtaaaaactt attcaagcgg gatgttcgtt cgtctagctt
    11941 ttgctgtagc aatcaatgtg aatcccgata ttttgattgt ggatgaagca ttggcagtag
    12001 gcgatcttga atttcaacta aaatgtatgg aaaaatttac agaaatcaag aactcgggaa
    12061 agacgatttt attcgtttct catgatatca gttcgattcg tcgtttttgt gatcgttcct
    12121 attggttgaa aaacggagaa gtcgtcgaat atggcgatac gatggaagta acgacgaatt
    12181 atgaaaactt tttaaagaaa aaatcagtta aaacagtgga tcggaataaa aaccaagtag
    12241 aacggttcgc cgctccagat atcgtcgacg tgacgaaagc ggaattgttg gataaagaca
    12301 aaaatccggt ggatatgctg gaacaaggag aaaaactata cgttaaagta acttatgaag
    12361 taaaagatga tacaatcaaa aatccggttt taggaattgc gctacgaaca gtcgacaatt
    12421 tttatgtatg cggacttaat actctactag atggtatcaa gattccttgg aaaaaaggag
    12481 agaatatctt ttatctggag tatgaaaagt taggtttgtt agctggtgaa tattattttg
    12541 acgttgcggt ctttgaagaa aatgcaacgg ttcctttggt atataagaca aagtacatga
    12601 acttgtttgt cagcggttct tatatcggag aaggcatcgt ggtcttagac cataaatggg
    12661 aagaagggac tcatagcaat gaaatatgat tttgaaatgg acctagatga acaaagcagt
    12721 gtgggaaaaa ttgccgcaca gatcaaacca ggaagtaaag ttttagagtt tggtcccgga
    12781 aacggccgat taacgaaaca tttaatcgga gcaaaacaat gtgaagtgag cattgttgag
    12841 ttagataagg aattgttcga ttttgtcagc gaatttgctc aagatggatt ttatggtgat
    12901 atcgaaagtt ttgagtgggc aaactattat gctggacaga catttgatta cgtcttgttt
    12961 gcggatgttt tagaacattt ggtagatcct ggaaaaacat tgaagaaagt cagagaattt
    13021 ttgaatgaag aaggagagat cctgattaca ttccctaact tggcgcataa ttctgtcatg
    13081 atcgatttgt tcaataacca attgccgtgg gcttcttatg gtctcctaga tgaaacccat
    13141 aattcttttt atactcatga tggcttccaa aaagtttttg aaaaagcggg attattcatt
    13201 aatattgaag attatttgta cttagctgta ggtgatacag aattaaaatc aacttatgaa
    13261 gaattgccgg aagctgtacg ctatgatttc aaaatgcgac catttggtga agtctaccaa
    13321 tactttttct cattgatgaa acatccagta gcacaaagtt ccattgcaga gccacaaaat
    13381 tctaattatg taaaagtgtt agaagtaacc cagcaaacga aacaagatga aacaatccaa
    13441 caaataccat tcaataattt cacaggggaa aatgagacac taagtttccc cgtatcagaa
    13501 acaacagaaa gaatggtgtt tcgcttcgcg caacaaccta gctttatcga attcagcgca
    13561 gaagctgccg gagaaaaact aacatttatc gattcgaatg ctgtagtcaa aacagtaaat
    13621 gattgttatt tgtttgatgg gaaagaatta ccagagttcg ttctaacgga tatttctggg
    13681 aaagaagtga ctatccattg tcactatcgc tttatcgggg aattgacacc aacaatgaaa
    13741 gaattgttgg aaacgattcg tcccatggct gaagtcacaa agcagctttc tgagaaaaat
    13801 gacgaattag aaagagaaaa tcatcatctt cttgaggaaa atacaagact agaccatacg
    13861 ttaaaaacaa ccacaaatcg ctattgcaca ttgttggaaa gtgatgaatg gacaataaaa
    13921 catagactag gacgacgtaa gaaagaaact tcgaaaaaaa tccaagaaaa agaactttct
    13981 atatgtattg acgagaaaat ctgggatgca gaaacaaaaa tattaaaaat catcggatgg
    14041 ggaatcgcta attcggatcg ccagcccttg tcctataagt tatcagctga ccaatcgcca
    14101 tttttcgaag caattcctgt ttcacgagaa gaagtcaatc aagcagaaca gctcgcaaaa
    14161 ggaacagaag cgggatttga gttacgaatc ctttgcgagc aagaacgttc attcttagtg
    14221 gaagcagtag ctcaaaatgg ccaatcatgg atcattgaaa tgtaaataat gattaactta
    14281 taaagataag gagtaaacga atcgtgcaaa aaaaagaaat tgccgtattt atcgatcaaa
    14341 tcactcgtga acgggccact ggcgatttgc ttattgtggg ctgggcaatt gatgaagtga
    14401 ccaaagaaat ccctacgata aaggtagaaa aagaaaatgt gattgctgaa gcaacacatg
    14461 tcgtaagatt ggatatcaat catttatata atcttgatgt taagacccaa agcggtttta
    14521 aggtacgttt atcagggaaa atgagaggaa aagccatctt agacttccaa acagcgagac
    14581 atcaaaatgg catagctgtg aaattaaacg gtaaataccc ttatgatgac ggcattgaat
    14641 cttcatggga aagaaaaaaa agattactga aaaaagggat aaattatgca aggactcatg
    14701 gcgtaaaaaa agcgatccgt cgggtcaaat tggaattgaa gccaggatcg atcgactatg
    14761 cagagtggat cagtcgtcat gagaaaccag atttgaaaga gcaaaggaaa gaagcacaag
    14821 gatttgcgta ccgtccattg atttccatac ttatgcctgt ctataacgtt gaaatcaaat
    14881 ggctcgaaaa atgcattgat tcggtactgg atcaaacgta tgatcattgg gaactatgta
    14941 tcagtgatga tgcttctact gatccagcta tccgaaagtg tctggaatcc tatcaagcaa
    15001 aagatgatag aatcaaagtc gttttccgaa aagaaaatgg acacatcagt ctggcaacta
    15061 attctgcatt ggaaatagca gaaggagaat ttatagcact cttagataat gatgatgaat
    15121 taccgccatt tgcattatat gaagtggcaa aagtgctaaa cgtacatcca gaactggatg
    15181 tgatctacag tgacgaagat aaaatcgatg cagatggcaa tcgctttgat ccgcatttca
    15241 aagcagactg gtcgcctgat acattgatgg gaaataatta tatctctcac ttaggcgtat
    15301 atcgaacaag cattgttaaa gaacttggcg gtttccgtaa aggctatgaa ggttctcaag
    15361 attatgattt ggtccttcgt gtgacagaac aaatccctgc agatcatatt taccacattg
    15421 accgagtctt gtatcattgg cggacgatcc caggatcaac tgcaagtaat ggcgaagcca
    15481 aaagttatat ctatgattcc ggagtgaaag cattgacaga tgccctgtct agaagaaaca
    15541 tcaaaggaag cgtacgtcct ggcagaattt ctggatttta tgagattgct tatgacgtct
    15601 tgcaagagga tttggtcagt gtgatcattc caacgaagaa tggctatgaa gatctaaaaa
    15661 cttgtgtgga ttcgatcatc gaaaaaacgt cttattcaaa ttatgaaatc attattgcgg
    15721 ataacggaag tacagatcca aaaatgcaag aattatttgc agaatacaag catcagctga
    15781 aagaccgctt tatcgtagaa ttgattgaca ttccattcaa ttattctcga attaacaatt
    15841 tagcagcaga aaaagcaaac ggcaaatact ttttattctt gaataacgat acagaagtga
    15901 ttgagccaga ttggatgacg gtgatggttt cttatgcaca gtttgatcga atcggctgtg
    15961 taggagcaaa actgttttat ccagatgata caacgcagca tgcaggagta ttattaggaa
    16021 tcggtggtgt tgcagggcat gcattgaata actatgatcg aacacattgt ggttatttcg
    16081 gccgattggt cattgacgtc aattacttag cagtcactgc agcttgtatg atggtcaagg
    16141 cagctgactt caatgctgtt aacgggtttg atgaaacatt agaagtagca ttcaacgatg
    16201 ttgacttgtg cttgaaagtt tatgaactgg gtcgttacaa tgtctatgca catcaagcag
    16261 aattgtacca tttcgaatct aaatcaagag gttatgaaga tactccagaa aaacaaagac
    16321 gttttgctgg tgaaatcaaa aaaatgcaag ataaatggcc agcatatatt gcgcatgatc
    16381 cattttacaa tgataattta acaaaagaag gaatcggcga cttttcgttg agaccagatt
    16441 aaactataat aaaaagctgt tatgttgtga ttcgctcgca cataacggct ttttatagtt
    16501 ttttgtcaaa aaaaggcaga aggtgccgac tcaaaaaaat aagtcgaggt tgggacagaa
    16561 gtgtttaact ccgagaaata agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa
    16621 tttcggctta tttaagaaga agttgcttct gctcccaccg tttattcgtt tttaaagaat
    16681 gagacaaaag tgtttcttac ttttgtccca cgctcttatt catgctcaga gcgccaatag
    16741 atacaaaatc atagccaaag tttcacgaac cctagctgtc aaagaatcga tattttattt
    16801 taatataggc tattttaaaa taaatggttt aaggtattcc tctacaaaag ccactctatc
    16861 aattatcatg ggtttaaaat aagaagtgat ggagataacc agattgttct tagcgtcaat
    16921 ataaagaaca tttccactat taccaattgc tgaatatatt tcgttctcag aatctaatac
    16981 ccaccataaa tagccatatc tcatattttg taagtcttct aaagcactgg ttctaggttt
    17041 gaacatctcg tttatccaca tgggtggtac gatttgtttt cctttataca tccctttatt
    17101 taggcataat acaccaattt ttgccagatc acttgccgat aaacacaaac catatcctgc
    17161 cgtcccgata tttagtggat cacaaaacca gacattggtt ttaggagact tatccattgt
    17221 aaactgctta tgttcttcag ctgtttcagc ataataattg acatggtttg gtatccctat
    17281 aggatcaaat agatacttat tggcaaaatc aacagttgtt agcttacttg tctgatacaa
    17341 caagccagtt aatatgtgta aacagacagt ttgatagttg aattcgccgg ttataccttt
    17401 tcttcctccc aataaatcta gtgatgtttt tgtccaatct tcacttgaac agacttttgt
    17461 ccatggatcg cctttacatt tgtagggtgc tttcattgtt agcaagtgct tgattgtaat
    17521 agcgtaaata gttttctccc cgcgtttcac tttatactct ggaaaataat ctaatacttt
    17581 atcatcgata cttttgatta atccttgctc taaagcaatg ccaatcagca aagatacaat
    17641 acttttcgtt gctgacatga tatgacaagt atcgtctttt ttataaccat tccattcatc
    17701 tgtataaact tctaccccat ccttatatgc gacaatttga catatatttg gttgtcgaaa
    17761 accaataaaa tcatgcaact cttgtttatt cataagatat tacctcactt tcttctataa
    17821 gcataatgtc attaaaaatc gatttcaatt ttttcgattc ttcacgtaat acaggtattc
    17881 ttatggacta gacagcgctt tgggaagatg gattctctga aaaatataaa ggttcacttt
    17941 atgagtggat aaaaaaagca gcaaatactt cgacttcttt aatttaccaa cattagtgaa
    18001 aatcgattca tctggagatt tgtaatttag agtccttgtc ttgattagag gtgtataaag
    18061 ctttttatta gacattttta aattccatta caatttgctt ataaactagg tttttaaagg
    18121 tgactatggt aagataatag agatattatt ttgtgattag gagtgtatat atgaataaaa
    18181 atggagaatg gagtgctgca cgacggatat tcattatatt aatggatgtt attgtataca
    18241 atgcagcaat atatagtagt ttcttactga agtttcgtgg agagatccca ttacgaaatt
    18301 tagaaacttt ccaacactca gcaattatta tttcagtgat ttttattgcg ttgaacattt
    18361 tgttgggtgc atatgttttt tataatcgaa tgatcagtga catcgtgttt gtcacagtca
    18421 tcggtcaggt attgatgaca ctagggatca tggtagtgac ttttgcgggc cgctggtttg
    18481 cttttccacg tcttgttatc ttactttcat ttgttctcgg tacgcttttc ttaagtatct
    18541 atcgtattct cgtatataaa ctttatttac gcgtaagtcg agataaaaag gtcgtcatta
    18601 ttgggttaga aaaggatgtc gcaccagcaa tccaaaattt tggttcaaag aaaaacaaca
    18661 aacataaagt cgaagcagtc attcttgata actattatgc aaacatcaaa aatatgatta
    18721 aagacatcga tattgtctat cttgcttctc atatcgaaga aactgaaaaa atcaaaatct
    18781 atcaattagt tactaaaaat gaaaaaaaat tgtttttgaa tacaacattt gaaaacttga
    18841 cattgatcaa tccaaatatt atgaatttcg aagatgaaag tattattgaa gcatctggat
    18901 ttagaatcca acctgaatat gaactattca aacgtttgtt tgatatcatc gtttcacttg
    18961 ttttattagt tattgcttca ccatttatgt tattcacagc aattttagtc aaagtgacat
    19021 caccgggacc aatcatctat aaacaagtgc ggatcacaaa aaatcaaaag gaattttcaa
    19081 tctataaatt ccgctcgatg accgccacag ccgaagcaaa atctggacca gttttagcta
    19141 agagcaatga tgcacgagta acgcctgtag gtaaatttat tcgagctgtc cgctttgatg
    19201 aattaccaca aatcttcaat gtgctgaaag gcgatatgtc gatcgtagga ccacgtcctg
    19261 aacgtccttt cttcgtcgat cagttcaatg cagaaaaccc ttattactac ttacggcata
    19321 atgttcgtgc tggtatcact gggtatgcac aagtctatgg aaaatatgtc tctgattaca
    19381 acagtaaatt acgttttgat ttactttata tcaaaaaata ttcattgatg cttgatttaa
    19441 agatcttgct gcaaaccatc aagatcctat ttgataaaat gtcctctcaa ggacttgaag
    19501 aagacgaaaa tgtgatctca ttggcagaaa ttgcttttcc gaatgaaaaa atctttcgct
    19561 agttgggagg cgtaaaaatg aacaatccga aattcagcat aatcgtacca gtatacaatg
    19621 cagaagcaac tattggtcga acgattgata ttctaaaaga attggattat gaagagtacg
    19681 aagtcgtttt agtaaatgat ggatcaacag atcaaacaca ggaaatcatt caagaactga
    19741 ttgaaaatga tgaacgtttt gaattgatta ctacgaccaa tcaagggccc ggattagcaa
    19801 gaaacgaagg aataagacag tcaaaaggag aatatctctt attcttcgat gcagatgaca
    19861 cgccagtaaa aacgatcttg tcagattatg atcatttgct agaaaagagt ccaaaagcgg
    19921 atcttgttgt tagttcgttt gtttttcgta caatggacga gggaagaatc gtttccgaaa
    19981 aagaaaactt agttacagaa tgtcactatc taactcacga agagtttata aaagacatgt
    20041 atcagttaat gaatgaacag ttgatgtatg ttgtatggaa caaatgctat cgtagcgata
    20101 ttctgaaaaa taaccaaatt ctatttaaaa cttataatag ttgtgaggat cgaatattta
    20161 acttacatta ttatgaatac tgtcatcaag tgatcatgaa cccaaaagta gaatatattt
    20221 atgaatttga aggtgggaag gggatcacta atcaataccg acctaacaag ttcgaaacat
    20281 ttaaagaatt ctattcatta gcaaatactg taacagacga tattaataaa gacggtatgg
    20341 cggcgcttct tttaaaaggg acaacttcag ttattttttc tatttatagt acctctgcaa
    20401 gaacttcaaa agagaagaaa gacgaagcaa agcagatatt gagtgatcca gtgatccgag
    20461 aagccaagca aattgctaag acggattctt cagcaaaaaa aataacaaag ttgctttaca
    20521 atatgccagc tccactattt ttatctgctg taaaagttgg aagcttcgtt gaagtgaaaa
    20581 tgccagggtt aatggctgct ttgaagcgag tatattagtt aatggaggaa aatataatga
    20641 aaagaatttt gcattttcag ggacgaatgg gtttaggtgg agcagagtct ttcatgatga
    20701 atttatatcg taaaattgat cgtacaaact atcaatttga ttttttgatt tatgaagatt
    20761 atgcagatgt tcaagactat cattcagaga ttgaaagatt aggaggccgt atttttgttg
    20821 tacctaatcc taagaaaaat atttttaaat atttaataga agtgaataag ttactaaaaa
    20881 aagaatcttt ttctatagtt cacaatcagg tttattttgg cgggggaatt aatttatggt
    20941 tagccaaaaa gaatggaatc agacagagaa ttgctcatag ccatgcaact gaagatggaa
    21001 aatctcaaaa tattgtaatg aatgtattgc gtaaattttt aaccaaattg ttgttacaaa
    21061 atgcaacaga ctaccttgcc gttagtcaag aagctggtga aagtctgttt caaaaccacc
    21121 cctttgagat agttcataat ggtattgatt tagagcttta tagtaaaaat tcagaagcta
    21181 aggttaataa gagaaaagaa ttggatatct caatgagtac ttttgttgtg ggaaacattg
    21241 gacgattaga gaaacaaaaa aatcaatctt atttgttaga gattttttta gaaatactaa
    21301 aaatgaattc tgattcttac ttgattttag taggtgaagg gtcacttaga aataaactga
    21361 aagccaaagc agtagaattg gatatagaga aaagagtaaa atttttagga gaaagaaacg
    21421 atattcccga gttattatct gtgatggatg tttttgtaat gccttctctt tatgaaggtc
    21481 tgccaatggt tggattagaa gcacaagcag caaaattaaa attagtctta gcagatacta
    21541 tttccaaaga tacaaagtta acaaataatg tggattttgt atcattagat gaaacaccac
    21601 aagaatgggc aaaaaaagta ataaaagagc cgtatgctaa taaaataaca caagaactaa
    21661 agagttatga tgtttcgtac actactgaac aaatggagag tatatatagt agagatcatt
    21721 aaaaatgttc aagatatttt aggagtgatt atttttgaaa atatgttatt tgatattggc
    21781 atacaatgat attggcaact taaagagatt ggtcaatcga ttgaacgaaa atgcagtttt
    21841 tattattcat atagataaaa aaactgatat agaaccattt attgaagcat ttaaatcata
    21901 tgataatgtt gaatttttat gtaataaaaa ccgtgttaaa atttattggg gtggtttttc
    21961 cattgtacaa gc
//
LOCUS       DAZKLA010000041        21630 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000041 DAZKLA010000000
VERSION     DAZKLA010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.041"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..1432)
                     /locus_tag="ACG85N_001289"
     CDS             complement(41..1432)
                     /locus_tag="ACG85N_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0224073.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            1689..5104
                     /locus_tag="ACG85N_001290"
                     /pseudo
     CDS             1689..5104
                     /locus_tag="ACG85N_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
     gene            5107..6528
                     /locus_tag="ACG85N_001291"
     CDS             5107..6528
                     /locus_tag="ACG85N_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0224074.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            6525..8402
                     /locus_tag="ACG85N_001292"
     CDS             6525..8402
                     /locus_tag="ACG85N_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0224075.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            8494..9336
                     /locus_tag="ACG85N_001293"
     CDS             8494..9336
                     /locus_tag="ACG85N_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0224076.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(9488..10363)
                     /locus_tag="ACG85N_001294"
     CDS             complement(9488..10363)
                     /locus_tag="ACG85N_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0224077.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            10570..11600
                     /locus_tag="ACG85N_001295"
                     /pseudo
     CDS             10570..11600
                     /locus_tag="ACG85N_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            11689..11994
                     /locus_tag="ACG85N_001296"
     CDS             11689..11994
                     /locus_tag="ACG85N_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFQ0224078.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            12173..14188
                     /locus_tag="ACG85N_001297"
     CDS             12173..14188
                     /locus_tag="ACG85N_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0224079.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            14190..14474
                     /locus_tag="ACG85N_001298"
     CDS             14190..14474
                     /locus_tag="ACG85N_001298"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0224080.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            14500..15873
                     /locus_tag="ACG85N_001299"
     CDS             14500..15873
                     /locus_tag="ACG85N_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0224081.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(15956..17971)
                     /locus_tag="ACG85N_001300"
     CDS             complement(15956..17971)
                     /locus_tag="ACG85N_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFQ0224082.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(17993..19207)
                     /locus_tag="ACG85N_001301"
     CDS             complement(17993..19207)
                     /locus_tag="ACG85N_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0224083.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            19427..20371
                     /locus_tag="ACG85N_001302"
     CDS             19427..20371
                     /locus_tag="ACG85N_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0224084.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            20667..21611
                     /locus_tag="ACG85N_001303"
     CDS             20667..21611
                     /locus_tag="ACG85N_001303"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0224085.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         7336 a         3966 c         4093 g         6235 t
ORIGIN      
        1 ttttatcagg gtggtttata catagctgtt ttttgattat tcataactcg aataagcgga
       61 tgagttcttc ttcatttgcg aatgacacac tatcatcaat caatatatga ggaacatggg
      121 cattcaaaat gggttgattc gtaacgatca gatcatactc gagaagattg tatttcgaca
      181 gctgatcttc agtaatagca gttgtctcag agtgaatttt atgcacagca agaatatcac
      241 tgatatattc ttcaatatat tgactatgtc gaagacttaa gtcgctgata atcagtattt
      301 ttttatcaga agtataatgg tgaatttcag gattaccgat aagcatccaa aagatatagc
      361 tagataaata ataaatcaga ttctgattca gtagagtgga ataaaattga agttctgcac
      421 gaaatccagc atatagacgt atctggcggt gttgcatctg ctgtgcaaag taagttgcac
      481 ggatcgtcaa atgagtcgta tcgtatggga aaatacggga gaaaaaataa atttgcgtta
      541 ataattgaac aacatattct tcgttttttt ctttgcaatc agcggatgaa aacgaacagt
      601 aggacatatt ttttcgtatc tgctcactga tttcattttt ttctttctcc gaaaattctg
      661 aaaaaatcgt attgacaaaa tagttcacta cttttatata gctgctgtac ttcaattttc
      721 ctaagagatt tttcaagatt aagaagtctt ttttatcgat atttatcaac tgtacataag
      781 agtctccagc tttccattca aatggagttc cttgggattc tctcatgaga tgcacaacaa
      841 aatacataat aaagaaagat tgctcatatg ggctttcttc ctgaaacaaa ttcatctgac
      901 aattattctt gaccataatt tctttgatct tctttttttc gattggaaat ggcaacgtgc
      961 gtcttccgta gtaacttaaa aagaaaaagg taacaaagat acgcaactgg agctcacttt
     1021 taaggctagt taactgaaaa ccttggtcaa cgatcacttt taataaatag ctttctaaaa
     1081 tttgattgca ttgtttcaac ttacgtgtaa aagaagaccg gctgatggaa attttttcac
     1141 aataatactc aatcgtttta cccggattga caataatctc atttaataaa cgactgtttg
     1201 tagaactttg cgacatttcc atcagaacat ttaaataagc tggtacattc aaatgttcaa
     1261 gacgataccc caatttttgt gaagattgaa tcgttacatc cgttccccag cggattttga
     1321 tctcttcgat ataccttaca aatgtagatt ctgaactacc aattcgtttg gcaagttctt
     1381 ttttagtcat ccacttgtca gaaatggaca gaattcgaat gattttttcc atctttctat
     1441 agtttaattc caacaacctg atcatatgtt tttctccctc aacccttttc tttcaatgaa
     1501 acgctcatta atcgaaggaa tcaacttcat aaatgaaacc aattggtatg aaaattggtg
     1561 tgattccctt tagcgccctt cgtataataa ttatcatatt aaaataaata aaattatgta
     1621 acgcctgttt attttaataa ttttcactat tcacgtcaaa acagaaaaaa agaaaaagga
     1681 ggggaaaaat gacgaccaca gggaagaaac tgaaagttat tttcatgctg ataatattga
     1741 gtttatcaaa ctttgtgcca ttatctgcaa tagcagacac tacagatgat ccaacagttt
     1801 tagaaacaat ttcagctgaa gtcatttcgg atcagtctgg aaaaaaagca ctgaacatca
     1861 agctaaatgc gaataacacc agtgctgaaa agatagaaaa agaaattggt ctagtcgaaa
     1921 attacttaag tgatgtggaa agaaaagaag gagatggcta tgcttatcag gtaaatagcg
     1981 ggaaaattac gttggaaatc tcatcaaaca ctaaacaaac tatcgatctg agttttccaa
     2041 tcgatccagc actttaccac agccaggcaa acaagctgat cgtcgataat aaagaatatg
     2101 acattattga tgagacagaa aataagaaag atacagatgt gtcagtacca aagccagacg
     2161 aaatagaaga agaatcatca aaagaaaacg aaaattctgt cagcccattt acattgccta
     2221 cattatcctt gccagctgtg agtgtgccat ctaatcaaac gattcctaca gaatatacaa
     2281 cagatgatca gggcacttat cctaaagcca gttggcaacc tacaggaaat acaaatgttc
     2341 ttgatcatca aggcaataaa aacggaacaa atcaatggga tggtataaat tcttggaatg
     2401 gagatcctaa tgatcggacc cattcgtata tcgaatatgg aggaaccggt aatcaagcag
     2461 actatgcgat acgaaagtat gcaaaggaaa caagtacacc cggattgttt gatgtttatt
     2521 tgaatgctcg tggaaatgta caaaaagata tcacgcctct tgatctcgta ttggtcgtag
     2581 actggtcagg aagtatgaac gacaataatc ggatcggtga agtaaagatt ggtgtcgatc
     2641 gttttgtcga tactttagca gatagcggta tcacagacaa aatcaatatg ggatatgtcg
     2701 gctactcaag cgaaggatat agctacagta acggtgcagt acagatgggt tcatttgatt
     2761 cagtgaaaaa tcaagtaaaa tccattacac cttcacggac aaatggtggt acttttacac
     2821 aaaaagcact aagagatgca ggaagcatgc tatccgttcc aaatggacat aaaaaagtga
     2881 tcgttttgct gacggatggt gtaccaacgt tttgctgacg gatggtgtac caacattttc
     2941 ctataaagta cagcgggtac acgcacaatc aagcagcaat tattacggaa ctcagttttc
     3001 taatacgcaa gatcggccgg gaaatacttc tctaatctca agaatctatg atgcacctga
     3061 ccaaaacaat ctatccagaa gaatcgacag tacgtttatc gcaaccatcg gagaagcgat
     3121 ggcactcaaa gaacgaggaa tcgaaataca tggtcttggc atccaacttc aaagcgatcc
     3181 ggcagctggt ctctcaaaag cagaagtaga gtctcgtatg cgacaaatgg tttcatcaga
     3241 tgaaaaaggc gatctttact atgaatcagc tgatcatgca acagatatct ctgaatacct
     3301 agccaaaaaa gctgtacaga tctcagcaac tgtaagcaat ggacaaataa atgatccaat
     3361 cgcagaacca ttcatttatc agcctggtac actttcagtc aagagtgtgg ggacaagtcc
     3421 tacaacggtc actccatcta tttccataga aggaaatacc atcaagagca atcagatcta
     3481 tttaggaaaa gaccaagaaa tccaaatcca ttaccaagtg agaatccaaa cagaaaatga
     3541 ggacttccat ccaaatttct ggtatcaaat gaacggcagg acaactttcc agccaaacat
     3601 tgataccaat gaattagctg aattcggtat accatctgct aaagctcccg gagtcagtct
     3661 tcacatcaaa aagttatggg aagaatttga caacaatcta gctgatcgtc cagatcaagt
     3721 tacttttgag attcaacggg aacatacgac aaatgctgca gcttggaaaa acggatatat
     3781 tcgaatcatt aaaccagcta aagatacaac aaatacgtgg gaacgtgcag acattgacaa
     3841 attatctgca aatagcggag aaagttatca agagatatta tcactacctc aatacaataa
     3901 tcaaggtcaa gcattcagtt accaaacaat caaagaatta cctgtaccag gatacgattc
     3961 tcaacaaata gatgcaatga catggaaaaa tactaaacaa ttcacaccgt taaacttgaa
     4021 aataacgaaa aattcctcta caggtgaaaa ggatcttatt ggcgctgttt tcaaattaac
     4081 aggagattct attgatactt tactaacaga tcatggcgac ggaacctatt ctcttccaga
     4141 aaatgtcaaa ttgcaaaaag aaatgaccta tacgctgaca gaaacaaaag ctccagaagg
     4201 gcatggatta agcaaaaaga ctacttggga aatcaagatc gcttctgatg gtacggtaac
     4261 cattgatgga aaaacagtca ctacttccga tgatacgatc cagttgacta ttgaaaatcc
     4321 ttttgttgaa gttcctgtag cagtacgtaa gtatgcgatg caagggacgg acaaagagat
     4381 aaatcttaaa ggagcagcat tttccctaca gaaaaaagaa gcaaatggta cttatcagcc
     4441 aattgacagc caaacaacga atgaaaaagg tcttgccagt tttgattcac tcacacctgg
     4501 taaatatcga gtcgttgaaa cagctggtcc tgccggatat gatacttcgc cgggaaatta
     4561 tgaattccaa atcgataaat atggaaaaat catttacacg ggaaaaaata ccgagatgac
     4621 aaataatgta tggacgctca ctcatcaaaa tcgactaaaa gcgtttgatc taacggtaca
     4681 caaaaaagaa gacaacggac agacattaaa aggagcaaaa ttcagactgc agggaccaga
     4741 aatggactta gaatcgccaa aagatggaca agaaacagat acctttctat tcgaaaattt
     4801 aaaacctgga acttatacgc tgaccgaaac ttttacacca gaaggatacc aaggtctaaa
     4861 agagccagtt actatagtta tacacgaaga tgggtcaatt caagtggatg gacaagatca
     4921 tgaatctgtt ctgtcaccag gagccaaaaa caaccagatt tctttagaca tcacgaatca
     4981 ggcaaaagta ccattacctg aaacgggagg aattggccgt ttaggaatct atctagtagg
     5041 gatgattggt tgtgcgtttt ctatttggta tctttttttg aaaaaagaaa gagggggcag
     5101 ctaaatatga aaaaacttgg ttggcttagt atgtgtctct tcttgttact atttaaacca
     5161 gcttttactc aggtagcaac agaaacagaa acagaaatgg ttcagattac tttacacaaa
     5221 ttgcttttcc caaacgggca actgccgaaa aatcatccaa atgacggaca agaaaaagct
     5281 ttattacaaa cgtatcgagg attaaatggt gtcacattcc aagtttatga tgtcacagat
     5341 tctttttacc atctacggga aaagggcaaa acggtagaag aagcacaagc agagatcgca
     5401 aaaaacggtg cgtcttccgg tatgtttacc gcagaagcaa caactacaac tcttaacaac
     5461 gaagatggta tcgcttcttt ttctctggcc gctaaagatc aagaaaaaag agataaagcg
     5521 tatcttttca ttgaatccaa agtaccagaa gtcgtcaaag aaaaggcaga gaatatggta
     5581 gttgttcttc ctgtacatgg acaaaacaat caaaaacttt caactatcca tttgtatcct
     5641 aaaaatgaag aaaacgacta ccctgatcca ccttttgaga aggtattaga agagcctaga
     5701 aatgatttta cgattggtga aaaaatcact tattccttgc atacgacaat tcctgtaaat
     5761 atccttgact atcaaaagtt cgaattgtca gatagtgcgg atgaagcatt aacgttttta
     5821 cctaatagtt taacgatttc atcgaatgga gaaaagctga cagaaggctt tgtcatacac
     5881 aagaaacctc acggatttga tgttttattt tcgatccctt cgttggaaaa atatgctgga
     5941 aaaaaactga ccatttctta tcagatgcag ctaagcagta cagcacaggc gaacaaggaa
     6001 atcaacaaca acggaacact ggattttggt tttggtgtca gtacaaagaa agtctctgta
     6061 tatacaggga gtaagcaatt tgtcaaaatc gagacaaata aaccagataa acgattagct
     6121 ggcgcagtat tccttattaa aaacaaagca ggaaattacc tccagcaaac agccaacgga
     6181 tacaagtgga caaagaacga atcagatgcg cttcacctga tttccgataa aaatggcgct
     6241 ttttcaattt ccgggttgaa aacaggaagt tatcgattaa aagagatcga agcaccttct
     6301 ggttatattt taagtgaaac agaaattccg tttaccattt caacttttct ttctgaggat
     6361 aaagaggcgg acagtatatt gaaagtagtc aataaaaaag aaaatagccg tccatttctt
     6421 ccaaaaacaa acgaaacgaa aaatacactt ttaggcgttg ttggtatggt attcgcaagc
     6481 tttgcaatct ggttgtttat caaaaaaaga acaggagtga aaaaatgaaa aatcataaaa
     6541 aaataaacgt tatgttagga gtccttttcc ttattttacc attactcaca aacagcttcg
     6601 gcgcaaaaaa agtgtttgca gaggagacag cagctcaagt catccttcat aaaaagaaaa
     6661 tgactgattt acccgatcct ttaatccaaa acagcgggaa agaaatgagc gaattcgatc
     6721 aataccaagg attagccgat atttcatttt cagtttataa cgtcactcaa gaattttatg
     6781 cgcaacgaga taaaggagcg tccgtggatg cagcaaaaca agcagtccag tctttgactc
     6841 ctggtacacc agttgcttca ggaacgacag atgctgatgg aaatgtcact ttatctttac
     6901 ctaaaaaaca aaatgggaaa gatgcagtct acacgatcaa agaagaacca aaagacggag
     6961 tgtcagctgc cgcaaacatg gttttagctt tccctgtata tgagatgatc aaacaagcag
     7021 atggctctta taaatacggg acagaagaac tagatactat ccatctctac cctaaaaata
     7081 cagtcggtaa tgatggaacg ttgaaagtta caaaaatcgg tactgccgaa aacgaagcac
     7141 taaatggagc agaatttatt atttctaaag aagaaggaac accaagcgtc aaaaaataca
     7201 tccaaagtgt cacagatgga ttgtacactt ggacaactga tcaaaccaaa gccaaacatt
     7261 tcattactgg tcattcttat gacatcggca acaatgactt tgccgaggca tctattgaaa
     7321 aaggccagtt gatcgttaat catttagaag ttggaaaata taatttagaa gaagtaaaag
     7381 ctcctgataa tgcggaaatg attgaaaagc aaacaatcac gccttttgag atcctggcaa
     7441 atagccaaac accagtagaa aagaccatca aaaatgatac gtctaaagtt gataaaacaa
     7501 cacctcaatt gaatggaaaa gatgtcgcaa tcggtgaaaa aattcaatat gagatttctg
     7561 tcaatatccc attaggtatc gctgataaag aaggaacgca aaacaagtac acaacattca
     7621 aacttatcga tactcatgac gctgctttaa catttgataa tgattcttca ggaacgtatg
     7681 cttatgcctt atatgatgga aataaagaaa tcgacccagt aaattattct gtcactgagc
     7741 aaacagacgg attcacggtt tcagttgatc cgaattatat tccttcatta actcctggcg
     7801 gtacattgaa attcgtttac tatatgcatt tgaacgaaaa agcagatcca accaaaggat
     7861 tttctaacca agcaaatgtc gataacgggc atacaaatga tcaaacacca ccgtcagtcg
     7921 atgtcgttac tgggggcaaa cgatttgtta aagtagatgg tgacgttaca tcagaccaaa
     7981 cacttgctgg agcagaattc gtcgttcgtg atcaagatag tgacacagcg aaatatttat
     8041 cgatcgaccc atccacaaaa gccgtcagct gggtatcggc gaaagaatca gcaacggttt
     8101 ttacaaccac aagtaacggt ttaatcgatg tgacaggtct aaaatatggc acgtactatc
     8161 tggaagaaac gaaagcgcca gaaaaatatg ttccattaac aaaccgtgta gcatttacta
     8221 tcgatgaaca atcttatgta acagcaggac agttgatttc tcctgaaaaa ataccaaata
     8281 aacacaaagg tacacttcct tcaacaggcg gtaagggaat ctatgtgtat atcggtgcag
     8341 gagtagtcct tctactgatt gctggactgt actttgctag acgcaagcac agtcagattt
     8401 agtctttttg caagaattgg aaaaaggagc agcggattac gcaaatgctc ctttttcttt
     8461 tattatcaaa cagtcatcac aaggagaaca caaatgaaac caaagaaaag gaaacttcta
     8521 gatctgctga tgcttttctt gcttctctca ggaataggga tactagctta tccatttgtt
     8581 agcgacgcgc tgaataatta tctggatcag cagatcatct ctcattatca gcaacaagcg
     8641 gtcaaagaaa atgaagaagt catggctaaa atccaagaaa acatgacaaa gaaaaaccag
     8701 caattagcaa aagaaggtgg aaatccaggt gcagatccct ttactaagaa aaaagaacct
     8761 gcgaaaaagg atagaaccta ttttgagaaa catacgatcg gtattttgac gatccctgag
     8821 atcaatgtcc atttgccaat ttttgatgaa acgaatactg tgctgctaga aaaaggtgcc
     8881 tcattattgg aaggaacgtc ttatcctacg gggggagaaa atacacatgc cgttatttcc
     8941 agccatcgag gactgcctca agcaaagttg ttcacagact taccgaaact gactttaggt
     9001 gattctttct atatcgaaat caatggacgg acactcgctt atcaagtcga tcagatcaag
     9061 acggtagaac cgactgatac aaaagatctg agaatcgaaa aagggcagga tttcgtgaca
     9121 ctcctaactt gtacacctta tatggtcaac agtcatcgct tacttgtcag agggcaccgg
     9181 gttccttatc atgcaaaaga agttcaaaaa gaagtacaga aagtatccga gaacaaacga
     9241 tttttcctgt atggattgtt agctgcatgt atactgattt tcctccttct tatatatctt
     9301 tttcgtcaat cgttcaaaat aagaaacaga agttagctat tcctcatttc tataatatat
     9361 ctattcgaat cgttccgtaa acaaacaggc tgaggtttga aaaagaaaac ttcagcctgt
     9421 ttttatgatg atatcaaaga gagcctatca aacactatct ttcttattac taaatgagga
     9481 tcttttctta agttttccga tattctttaa gaaggccttt ttgcatcaat tcttgtttgt
     9541 ttttcaagtt gcggatattc tcttcaaaat tttgagaata tatcactgag aataacgtat
     9601 ttaatacgta atcgaaggca atttgagagg agaaagtcgc tactttagca aaatcatatt
     9661 cctcctgcga tataacaaga cacatatcag cctgtttggc catttctgaa tgctgatttc
     9721 cggtaatcag caaggtaggg gcctctacat gcttcaaata ggtcattatt cgttcataat
     9781 gatggatgcg accgctgtag ctaataaaaa tggcgcaatc ctcgctagtc aaattggctg
     9841 cattccaaga agaatcactg tattcttcag ccatgatcaa aaacttattc aacttgacca
     9901 ttttattctg gaatttcctt gccgtgattt gagaatcacc ttttgcaaaa agaaagattc
     9961 gctgtgcctt gttcaatact ttaccgattt gatctaaatc ttcatgttca atctgcaatt
    10021 gtgctttttt tattgactgg atcgataaat ccgccatttt ctttgcaatt tgctgagtgc
    10081 tatcttcact tgaaaaagga aaattaggat caatcgtttg attgaactga gcaatcaatt
    10141 gatgaacttc ccgccccagc tcgaaacgga attctttaaa tccgctgaat cccatttttt
    10201 tgcataaacg aatgatagct gaatgggaag tatacgtagc ttttgctaat tcttcaatcg
    10261 tcatgtagac tgcagcttcg atattcctac ggatgtaatc gacaatccgt ttctctgtcg
    10321 tggtaaacaa ctcttgtttt gttagttttt cttcaataag catgatctga cctccaaatt
    10381 ctatcttact gataaacact tggttccgct actatctttt tgatttttat ccttactttg
    10441 aacattaatt tcaccaaacc gaatgataac aagtgttttt ctgaactttt ttaccagaat
    10501 agctattata aatactttct gcgcttttct aggatatact gaaaacatca ataaaaggag
    10561 gcaccactca tgcttacaat cgcttatctt ggcaatggaa agagtacaaa tcgctatcat
    10621 ttgccttttt caagaaaact tacgaacaaa atcaatatca aaaaaatttt ttccccatct
    10681 gaaccagtgt ggaaaacatt cgaagacatc gaatatactg atcagctgtc tgatctttgg
    10741 gatgatcctg agatccaatt agttgtcatc actacaccta gtcaatttca ttatgaatat
    10801 ggaaaaatgg cactcgaaca tggtaagaat gtactagtag agaaaccttt tgcagaaacg
    10861 tcaaaggaag cagaagagtt atttgcatta gcaaaagaaa aaggtctatt tatccaatgt
    10921 taccaaaaca gacggttcga ttctgacttc ttgacagtcc aaaaagtaat cgaaagcgga
    10981 aaattagggg atattttaga agtagaaatg cattatgatt atttcagacc ggaagtacca
    11041 gaaaatacgc atgaattttc tactgcgaac agctatttat atggacatgc ttgtcataca
    11101 gtagatcaag ttctttctta tttcggcgaa ccagatgata tcagctatga tgtacgtcaa
    11161 cttttaggga aaggacgaat gaatgattac ttcgatctgg attttttcta tggaaatatg
    11221 aaaatttcga taaaatccag ttatttccgc atcaaagaac gtccaagttt cgtcgtttac
    11281 ggaaaaaagg gatgttcgtg aaacaaacaa aagatcgaca agaagaacat ctgaaaatgt
    11341 tttacatgcc tgatcatgaa gactttggta tcgatttgcc cgaacattat ggtgtattga
    11401 cctatataga tgaacaaggg agttatcatg aagaaaaagt aatttccgag gttggtgatt
    11461 acagccgtgt ctacgaaggg atttacgaga cattagttaa tggcgcagaa aaagtcatca
    11521 aagatgaaga aacaatctta caaatgaaaa ttttggaaga aggaatcaaa agcattcaag
    11581 aatcagaagg aaaaatatga catatttgta agaatcgggt ttgttttttg acgcataaac
    11641 tgtcttattg tacgatgaaa aagtatagaa ctactaggag gaaccaacat gacaaattat
    11701 gacggtgaaa aacacaaacg attaagaggt tcaatggccc ctaaaacaga agaagaagtg
    11761 gctttagcag gaactaattc tggtgaaaca gaagacaaag aagaatcatt agctggcaca
    11821 aactctggtg aaacagaaaa taaagaaaca gctttatctg gttcaaattc tgtagaagag
    11881 gaaacaaagc ctaatgagcc caatcacttt gaaaaagata tcgaaaaagc ggttgaagat
    11941 gtcaaagaaa aatttgaaga actgacctca aaaaataaac atgatcatca ttgatcggac
    12001 gaaatcaatt cataaaagca ggagacattt ggtcacctgc tttttttctg tatccattcg
    12061 atagaaatcg ttcggtacag tcgtaaaaaa aatccaactg taagttgtac caaacacccc
    12121 tcgatttaaa agcgctacca tactagaata taagtaggag gtgaacagcg ctgtgaacta
    12181 caaaattagt tatctcctga aaacaccaag agaaatcagt ctagaaaaag cagcagagat
    12241 catcgaagta aatacaagcg aggcgatcaa acaattaact ccttttgccg aatatgtgga
    12301 tgatacccgt atccgtataa agaaattccg agcaatcgac tgggcagaaa aatttttgga
    12361 tattactcga tttgaagctg tgtataccga acaagagcga agagatcttc tttatttgat
    12421 ggtttattcg actgcaacgt ctgtttctgt ttttcatttt caagattttc ttcaggtttc
    12481 taaagggaca gtcctgtcag atatcaaaaa gataagaaat gaattagaag tggaagattg
    12541 tcagctgtcg tatactagga aaactggttt taggctgcta gggtcagaag caatattaag
    12601 aagagaagca aaaaatcgca ctgctcgttt gatgcagtca cttgctggaa gatttgggct
    12661 cacgtattta accgcaaccc tttccattga ttattacgcc aaagttagag atttgctaga
    12721 taaaacgatt tcactatcag atcttcgatt cgcaccaaat cgaatggatg aattagctta
    12781 ttatttgagt ttgacaacca ttcgaattgg aaaaacatcc atggaactgg taaaagatgc
    12841 tgaactgttg gcttctcttg cagtcactga cattagtcga caagtattga ctggccttgg
    12901 ctattccgaa catcaaaatt cggagattat atttttctct ctttgcttag catcgatcgt
    12961 tgaaggagat gtccaggagc ctgcacttga ttttttgctg gaatgttctt cagaagtcat
    13021 ccatcgcatg gaattcctga tggccgtaga atttgcttcg tttcgtgaat tattgatgaa
    13081 tgtcttttat cacttggtcc ctgcctattt tcgtatttcc tattcttttt atttacctaa
    13141 tgtgatgatc gatcaaatta agcatcaata tgcttccatc tacgaaatga caagaaaagc
    13201 attacttccg ttagaaagac gaatagggaa gagcatccct gaagaagaga ttggtttttt
    13261 cactatacta tttggcggag agatcagaaa agcggacgca gaagaaagaa atagaaaaat
    13321 aagagccgtg atcgtctgtc caagtggcat tagctcttca ttgatcttaa aaagtgaact
    13381 tcagcaatta ttcccgatga tcttttttac agagactaat tcagctgacc gcttacacga
    13441 ggttgacgaa acttcttacg atattatttt ttctactgta cctctcacac ttactgataa
    13501 gaaaaagtct ctctatgttc tgcatccaat catgactgac ttggaaaaaa atcagctgat
    13561 caatcaagta caaagagatt ggctgatacc aggattttca atgccttctg cccaagaatt
    13621 gttgtcggcg ctactgcctt atatcgaatt aaaagaaggc gtagacgaag aaaagctcta
    13681 tcaagtatta aatcgtaaaa tgaacaaact tttagaaaag aaggaggatc aacgtcctat
    13741 gctatctgaa ttattaacag aagaaatgat ccaattatcg gatcaaccaa aaaactggca
    13801 agaagcaatc acatttgccg cacagccgct attatcagaa aataaaatag aagaaagtta
    13861 tatcaaagct atgatcgagc gcgtggagca gtatggtgcg tttatccata ttggcgatca
    13921 catcgcatta cctcatgctc gtccagaaga tggcgtaaac gaagtaggga tgtctttgct
    13981 gaaacttcaa caacctgtgg atctgcttga taatccagcc catcccatta gtttgttcat
    14041 ctgtttagct gctatcgata atgaggcaca tcttcgggct ttggcaaatt taacaaagct
    14101 actttctaat aaacaaaatc ttcaagattt attacacgca tcaacaaaag aagaaatatt
    14161 agcgatcata agaaaaggag acgaatgata tgaaaatttt tgcagtatgt caaagtggat
    14221 taggaacaag ttttatggta gagatgaaca tcaaacaagc acttgcagat cttggagcag
    14281 atgtggacga gtttgaagta agtcatacgg atgttggaag tgtatctagc gatatggctg
    14341 attatttctt cattgagcat tctttgaaag atacgctaac ttctatacct gatgaaaagc
    14401 tcgtaccttt gcaatcgatc attgacagcg aagaagtaaa agaaaaagta acgaagatct
    14461 tgtcaaaaga ataaaaatga ttcaagggag gaactatcaa tggataaatt gttgaatttg
    14521 tttgtgtcaa tcgtcagtca gccctccatc ctcgtttctt tgattgcact gcttggtctc
    14581 gttcttcaga agaagaaatt ttcagaagtg atccaaggaa ctatcaaaac atttgtcgga
    14641 ttcctagtac tgatcggcgg tgctggttta atcagtaatt cactaacccc ttttgcttcg
    14701 atgttccaaa cagctctgca tactcaagga gtagttccta gcaatgaagc agtagtggcg
    14761 attgctttgc aacaatacgg tacacctact gctttgatca tgctagtagg gatgatcgtg
    14821 aatattttac ttgctagatt cacaagatat aaattcattt ttcttacagg tcaagcaatg
    14881 ctttatgttt cttgtctaac agcagtcatt ttagtaagcg cggggtttac tgcaaatttg
    14941 tggatgatct tattaggcgg ggtcttcgaa ggaacactat taactattac gcctgctctt
    15001 tgtcaaccgt ttatgaagaa catcactgga aacaatcaag ttgcgttagg gcataccgga
    15061 aatatcggtt atgcttctag tggatggatc ggaaagtttt ttggaaacaa agaccgttct
    15121 acagaggaca taaagattcc taaaagtttc agctttttgc gagattctac tgtatcgatc
    15181 atgctcttga tgtcgatcgt atatgtcgca cttgctttgt tagctggtcc tggatttgtt
    15241 gaaagagaac taagcaatgg agacaacgct gtaatctttg cactgatcca agctggaaca
    15301 ttcacagctg ggtttgttat cgtccttcaa ggggttcgaa tggtgttggc agaaatcgtt
    15361 ccagcattcc aagggattgc taaaaaactc gtacccgatt ctaaaccagc attagatgtt
    15421 cctatcgttt ttccatatgc acctaatgct gtattaattg gcttttttgt tagcttcttt
    15481 gtaggaacac tatccatgtt tgctatgata ggcttgcaaa ctacagttat cattcctggg
    15541 gtagtaggac atttcttctg cggagctgct tctggtgtct tcggcaactc gactggcggt
    15601 agaagagggg cggtattagg ttctgctttc aatagcttat tgattagttg gctgccatta
    15661 tttatccttc cagtattagg agatcttaaa atcgcttctt ctacatttgc cgatactgat
    15721 tatctagtac caggacttgt acttgggaaa ctaggagaat acggtcaaat agccttgaca
    15781 gttggaatca tcgctttctt tttacttgtc gtattatcta gctttctttt tgccggaaaa
    15841 agaacaaaca ttgtttctaa agaacagaat taagcaaact tcaaaataaa aattgcttgg
    15901 acaatatcag aagaagattt aaagtcttct gatatgtcgc aagcagtttt ttgttttatg
    15961 catttatttt tgaatagctt gttaagacaa gtggctgttt tgctagaaga ggataacttt
    16021 taccattcat tttcactgtt ccgcctgttc tatcagaagt aatcacgatc ttatcttttg
    16081 ttttttcaag cgaaatcttt ccatctttga taggtagcgt acaattcagt gtttcaaatg
    16141 aatctagatt aggttgtatc ataaattctt cataacctgg ctttgttgga tacaaaccga
    16201 taaaatagcg tcctaaaaga tagattgggc ttgctcccca tgcatggcat aagctttttc
    16261 cataaggatc tccatacata ctgtactttt catttcctgt ttgagtaggg tcgtattctt
    16321 cccagaaagt taccgcatca tgagataaca tccctcccca ataatcaaga atcgtttggt
    16381 agacgatatt cgtctgtccg agtttgcata aagcatcttg ttcatagaat ttgaaatatg
    16441 gagtagtaat ctgtgtgata gtgtcattca ataagacatt ctttaagatc gattgctgtt
    16501 ggtctttgtt agcaaaatcg aataaaacag cgaaaatatt cgcatgacgc gtcacatttc
    16561 ttctcccaga ttcatagcaa tcgataaaag cgccaagttc ttcatcccaa aagtagtcaa
    16621 ataggttttg tttcaatagt tgatattttt cttgatagag atgagtatga taattttcgt
    16681 tctctccata tgtttcagct aagatatctc cgcaagtgat catggaatga taggctttca
    16741 gtaataagac ttgttctgca gcaagcgttc cttctttatc catttcagac caatcaataa
    16801 atatccaatc ttttgctctc ccataaataa accctaattc attcgtttgc tccatcaagt
    16861 atctcattaa tgattgcatc ttagggtaga cgatgcgtaa aaattcatag tctttcgtca
    16921 tcatatagtg gttttcgata ccaatcaacc acaagatcga atagtcaacg atagtattca
    16981 tatgctgctt gatttcattt tgtcctctta atgctaatat cgttcgttta ttgatttcct
    17041 cgtcaaaaaa aagatattga ttgatcatga atgattgata cgcatctcca gaccaaatcc
    17101 agcgatctcg tttgataccg tcgataaaaa acaaatcact acataacttg aaggcttctt
    17161 ctgatactga ccagatctga ttcagtaaag aagaatcgct tgaaaaagca gcttgtttcg
    17221 aaagagataa aaattcatga aaaacttgaa gttgaaccat tccagtagaa acctctggta
    17281 taaacaaata gcgaaaagcg cgtttacgag ctttcatttt tcttgtcatt cctatttgct
    17341 tatagtagca ataatcgata tccatagctt ctacatcgga ctctccataa catagagtga
    17401 tagattcgat atggtcatcc agagatatta gttctacaca gccattcact gctcgcccaa
    17461 aatcgaataa gattcctccg ccaatctccg tttcagtgac tggatggacg cattcttttt
    17521 gataatagat ttgatttgga tcttttgcga tatcgacata aagcggactc cagccagctg
    17581 gatattcttc aataaaatta cttgctgtcc agccaatatc tgattttact tcgtctccct
    17641 cgataaacat cgctggtagc cctgatgtat gtcctacaaa gatacgaatt tttgctttac
    17701 cagctggaca agtcagccat tttccacatg gatgtttttg tccattaatt tctacatacc
    17761 caactccttg gatcgtcact ttaaacatgg aaggttgatc aagaaagtaa tatctcttaa
    17821 acttgacgtt ccgatggcaa tcatccatgt accagtacgc tggccagtca aaacctcgtt
    17881 cttcccgttg gaaattttgt agcagtccat ggcggatctc aaagtctcca ggataccaaa
    17941 gccatctatc tttatgtttt tcatttgtca ttttacacga cctccatcaa atttattttg
    18001 ttgtcaattg ttcttttatt tcttcatcta ctcttatcac tgtctttcta ttcgtcctgt
    18061 gtactactag aaggctaata agcgcgctaa tcaacagcaa gatactgcca ttgacgaaaa
    18121 cagcaagtgt tttactccca agagaagcaa aagagagaaa gagcaaacca tatggtgcaa
    18181 tgaaagtacc taaattagcg ccaaccaata agattgaagt attgaaactc tccgcgtact
    18241 tgggcgcttg gatacttact tggttgaata agtaaggaat gaataacgaa aaagaaattc
    18301 ctgttaggat cgcaccaaac ccaatcacat acaggtttat cacagtggca atcaatacaa
    18361 agccgattcc catcagtagt aaagaaagtg taagcatata gcgatgtgct tttgtctgaa
    18421 gccagccaaa acaaactccg gcagccatac ttgccaattg catgattgtt aagatattcg
    18481 ctgcattagt ttcgtttcct agagaataag tcgtcaggac tgtggctaat ttaatcgata
    18541 cagcattata taccacgatg accagcaaga gaaaaatacc ataactaatc attttaggat
    18601 tcgttttgat tacttgtctt gttggggctg tgccatgcga ttgaagttca gttgtttcag
    18661 gtgtaggaac aaacaaaaca aacaagacaa gaataggtaa gctgatggcg tagactaaaa
    18721 aggtatattg ccaaccgaac tgtactagct gtcctgcaac aaatgtcatt aaagctgcac
    18781 caaatccttg aaatgcactt tgaaaaccaa tcatagaggc tcgttcatct ccgtgataaa
    18841 aggcagcaat catgctgact gccaaagaat tgatcaatcc aaagccaata cctagtccag
    18901 ctcttgaagc taagacaata ctgtaacttt gtgagaagag ggggaggata ccacaaatcg
    18961 atgcaatcac taatccgatg atcacagttt gtttttgtcc gattttccta gcaataaaac
    19021 tactaatcaa aacaaaaata gtgaccataa aagagggagc agtcgttaat aattcaacag
    19081 cagataaaga acgcgtggca taagtagctg acatactggg aattgcagac gtgactgcac
    19141 cagcagaagt caaaataaga gagactgaca gcagagaaaa tttcatcatc tttttattca
    19201 taagcatgtt cctttcattt ttgtaagcgc ttatcaaaaa tatagtatga agtggttatt
    19261 ctctcaatag cggaatcaca ggaagttcat gtcccaacca tagaaagtct ttatgaaacc
    19321 aaaaatagct ttgcatataa tagcagtaca gcgtgaacta ataagcgaaa gaaaccgctt
    19381 acaaaaagga gggagtcaat tggaactagc tgaattagac atttttttgc gtaacgaaga
    19441 atcaatcgaa actctgcagc gtgaacaagg aatgaatatc aatgatttaa acttagctta
    19501 ccatcttcag acaccgccaa aaatccaccg aggggatcta tttcaacagg gaaatattca
    19561 tatcagtaaa caccgtcgat atgcggaagt tcccgagcat acacatgatt ttgttgaaat
    19621 caattatatg tatagcggta catgtttaca aattattaat ggagaaagga tagagatgga
    19681 agaaagtgac gtggtgatca ttgataaaga agctactcaa agaatcgaat acacagggca
    19741 agaggatatt ttaatcaaca tcttattaaa agaagaaacc ttatctacag agatattgaa
    19801 tcatttggca agcacaaata atctactttc ttcttttttg ataaatgcat caaaagaaaa
    19861 aggggaacat acacaattca ttcattttcg cggcggaaaa acaagtcgtt tgacgcattt
    19921 gattgaaaat atgctgttga tttattttga tacatacagt cataagatcc gttcgttaaa
    19981 tctctataca tcactattac tgatcgaatt gactcattta ttagaacaac aaagcttttc
    20041 atcagcagat ggatgcgcag aaaatgagat gcttagagta ttagattata tcgataagca
    20101 ttttacttct tgtacgttaa aagaaacagc agctcatttt ggctacaatc cggtctatct
    20161 tagtaataaa ttgaaaatgt attttgatgc ttcctttcaa gaactggttc taaagaaaaa
    20221 attcgacttt gcgttggaac tgattcaaga aacgagtttt tcgttggaag aaatcgctaa
    20281 tcgtatcgga tataaacaaa cgacttcttt attcaaatta ttcaagaagt attcaacatg
    20341 tactcccaat gaatggagaa ataaacatta agaagaaaat ctttcactaa agcatatgaa
    20401 ggaaaattgt tttaggtgcg attgctgtct tattatatcg ttgcagcgaa tttttttgga
    20461 tttattttca gttctaaact tttggttgta ttatcagggg atataggtgt aaacatttgt
    20521 catttatctg ttatgcgtat tcaggaattc gtttccatca aaaattataa taacaactat
    20581 tataattaac atgttgataa aatgaataaa caagactata cttttcatga aagtaacaaa
    20641 tagttatgaa aaggagctgt ttattcatga aaaaaacaag tcgtaaagta gtaatcgtcg
    20701 gtacaggttt tgttggaaca agtatcgctt atgcaatgat caaccaagga atctctaatg
    20761 aattagtatt gatcgatgtg aatcaagaaa aagcagaagg ggaagcattg gaccttttag
    20821 atggtatggc ttggggcgat gaaaatgtgg ctgtatggtc aggtggctat gaagaatgta
    20881 aagatgctga tatcgttgtt atcacagctg gtatcaacca aaaacctggt caatctcgtt
    20941 tagatttagt taaaacaaat gcgtcaatca tgcgccaaat cgtaaaagaa atcatgggat
    21001 caggattcga cggtattatc gttgttgctt caaacccagt agatattttg acatatatcg
    21061 cttggaacga atctggtctg ccaacatcac gtgttatcgg tactggaaca acattggata
    21121 caactcgttt ccgtaaagaa atcgcattga aattaaaagt tgacccacgc agcgtccacg
    21181 gctatatctt aggtgaacat ggtgattcag aagttgctgc atggtcacat acaactgtcg
    21241 gtggtaaacc agtgtttgaa attgtagaaa aagaccatcg tattgcccaa gatgaattag
    21301 atgtgattgc tgataaagtc cgtaatgctg cttatgagat cattgatcgt aaaaaagcaa
    21361 cttactatgg tattggtatg agtactgcac gtatcgtaaa agctatcttg aataacgaac
    21421 aagctgtatt gcctgtatct gcttatttaa ctggtgaata cgacgaaaaa gatatcttta
    21481 caggtgtgcc atcaatcgtt gacgaaaatg gtgttcgtga agttgttgaa ttatcaatca
    21541 acgaagaaga aaaagcaatg ttcagcaaat caacaagtgc tttaagagaa gtattgaaca
    21601 ctgtattata agaaacaatg aatatgaact
//
LOCUS       DAZKLA010000042        20957 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000042 DAZKLA010000000
VERSION     DAZKLA010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.042"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..563)
                     /locus_tag="ACG85N_001304"
     CDS             complement(<1..563)
                     /locus_tag="ACG85N_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFQ0224086.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="ACG85N_001305"
     CDS             complement(750..1400)
                     /locus_tag="ACG85N_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224087.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACG85N_001306"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACG85N_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFQ0224088.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACG85N_001307"
     CDS             complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACG85N_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFQ0224089.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACG85N_001308"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACG85N_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFQ0224090.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="ACG85N_001309"
     CDS             complement(4926..5657)
                     /locus_tag="ACG85N_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFQ0224091.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="ACG85N_001310"
     CDS             complement(5661..6926)
                     /locus_tag="ACG85N_001310"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFQ0224092.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACG85N_001311"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACG85N_001311"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFQ0224093.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACG85N_001312"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACG85N_001312"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFQ0224094.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="ACG85N_001313"
     CDS             12268..13770
                     /locus_tag="ACG85N_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFQ0224095.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="ACG85N_001314"
     CDS             13772..14509
                     /locus_tag="ACG85N_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224096.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14680..15873)
                     /locus_tag="ACG85N_001315"
     CDS             complement(14680..15873)
                     /locus_tag="ACG85N_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0224097.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15952..16158)
                     /locus_tag="ACG85N_001316"
     CDS             complement(15952..16158)
                     /locus_tag="ACG85N_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFQ0224098.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16151..17293)
                     /locus_tag="ACG85N_001317"
     CDS             complement(16151..17293)
                     /locus_tag="ACG85N_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0224099.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17805..18440
                     /locus_tag="ACG85N_001318"
     CDS             17805..18440
                     /locus_tag="ACG85N_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0224100.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18594..18671
                     /locus_tag="ACG85N_001319"
                     /pseudo
     CDS             18594..18671
                     /locus_tag="ACG85N_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            18985..19032
                     /locus_tag="ACG85N_001320"
                     /pseudo
     CDS             18985..19032
                     /locus_tag="ACG85N_001320"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19048..>20957
                     /gene="tet(M)"
                     /locus_tag="ACG85N_001321"
     CDS             19048..>20957
                     /gene="tet(M)"
                     /locus_tag="ACG85N_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFQ0224101.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFN"
BASE COUNT         6167 a         4298 c         3721 g         6771 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc tctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaaa ataaattata
    14521 tatgtctttt tgactatctt tttatatctt cttaaatgtc cgcaaagcct tattttatag
    14581 gctctacggg cattttgctt ttgtggtaaa cctcacatat ctaggtctat cttcttatat
    14641 tttcactatc aagcgtggtt aaaatcgtgg taaatacttc tatgcaatta gacgctgaac
    14701 ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga tactgatatt
    14761 tgaatgcccc atgatatact gtaaatcttt tgggttcatg ttcttgcttg ccagtcttgt
    14821 gcagaatgta tggcgtagcg tatgcggtgt gatatgtggc aatggattgt ccttgtggtg
    14881 cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatcg caactttggg
    14941 cttgccctta tgattgacaa ataggaagtt gccccgtccg tctatcacaa atggttctgc
    15001 ctttggacgt tttttcataa cctgttgaaa tgcctgtatc gtttctttgc ttaatggtac
    15061 ttgccttatt ccgctttttg ttttaggcgt ttcaatataa tagccctgtt ccttgctctt
    15121 tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag
    15181 tccgcacagt tccgaaatac gaagtcctgt ctttaacagt atcagcacat catcataatg
    15241 cttgtgatac acattgtccg tcttgatgaa tgagagtaag gcttgttcct gttcctctgt
    15301 caatgcgact ttctttttgg tatcattttc taggacttca ctcaacttga aatcaaaggg
    15361 atttttcctc acataatcat cttgaatggc tatgtagaat gacgctttta acgagcgttt
    15421 atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcctt
    15481 agcgtcagag ggtttgattg tatcaatgct cctagcacct aacttgtcct ctttcaataa
    15541 ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgccc tctgtgcgtt
    15601 ctgtttggca taaagttggc aaagcgtcat tttcttgcca gtgctgtcga taccgtcctc
    15661 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt
    15721 tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttccaaatgc
    15781 gtctacatat ttatataagt attttctgtc tgttcgttgg ctctctccag tatgcaggat
    15841 acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
    15901 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagatagc
    15961 gtccaattta tcaatgacct gttcaaactg tcggcgttta atctgtatgc ggttgccatt
    16021 cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc
    16081 gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
    16141 atcctgtgca ttactcataa aacaccccct cactttcgat tacgtctgct atgtccgtac
    16201 acatctgttc cacaagcatt tcctgctttt cagagaggtg ggtacattca tcaatcattt
    16261 tcatgtagtt tgagcctgtc agcctgtcga tttcctctat catcacgatt gacggcatga
    16321 cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacgggg ttcatttcaa
    16381 aaggtaaact gtcatgggaa atgaatatct cccacagcgg atagtcggga aattgcacaa
    16441 aatcagtaat aaggtaaaac accagcccat gagggttata tgtcaataac aattcctcaa
    16501 ctgcctgtac tgcttttgta ccacgcagac gaatttcaaa acgattgata atatccgtat
    16561 gtttcttttt ggttgcctgt tccttttgtt tttcatacag gcagaagtat tttgtgcttg
    16621 cctttgaacc gatataaagg gtgcttgcta aattacggtt ttttccgctt agttttccac
    16681 cctgcacatt ttcataattt ttacagcgac aatccgcacc gccatttctg tatttttcag
    16741 acaaaacggg aatatccaac agaccacaca tatcattgat tgccaagtca aaacgtcgga
    16801 taacaccgtc acagtcaaga cagcggttta aaaatgaata ccaatctctg ttctgtgcct
    16861 gcaagacata ttccatgttg cgtgagcctg ccccttttaa ttccaagaat acgcccatgt
    16921 gttcgtcatc agaagccatg accttgattt ctccgtaggc gtaatgctct ttatagccat
    16981 aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa
    17041 tttccaatgc gtcagtcgtt ggataacgga cagacagata atcaacgagc agataaaaag
    17101 gcttatcgca gatataataa ttcagacaac gcaggataat attttgtgtt tccttgctgg
    17161 cgttggcttt tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg
    17221 caagttctcc ttgtgaaaga ccgcttgcaa gacgtttgtt cttcatttca agtgcaaatt
    17281 ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaacctct
    17341 attcaaaaag tgtgatatgc actatcttct taaaacttct cattactact cacaactttg
    17401 ggattgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg
    17461 gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg
    17521 tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag
    17581 ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa
    17641 tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta
    17701 caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc
    17761 atattaagca tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa
    17821 tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac
    17881 aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag
    17941 ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc
    18001 ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc
    18061 agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact
    18121 tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta
    18181 cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta
    18241 tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac agtatggata
    18301 tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga
    18361 taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt
    18421 ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc
    18481 cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt
    18541 ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa
    18601 aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt aattggaaga
    18661 ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg tactctcttt
    18721 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtatcta
    18781 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    18841 tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgta cccagtttaa
    18901 gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct ttgtatgcct
    18961 atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt
    19021 ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
    19081 gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
    19141 gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag
    19201 agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac
    19261 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
    19321 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
    19381 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
    19441 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
    19501 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
    19561 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
    19621 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt
    19681 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
    19741 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
    19801 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat acgcctttat
    19861 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
    19921 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
    19981 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
    20041 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
    20101 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
    20161 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
    20221 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
    20281 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
    20341 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
    20401 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
    20461 tcgtttcaaa atgcagttat ggaggggata cgctatggct gtgaacaagg attgtatggt
    20521 tggaatgtga cggactgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
    20581 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
    20641 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
    20701 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
    20761 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
    20821 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
    20881 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
    20941 cgatatatgt tcaataa
//
LOCUS       DAZKLA010000043        20757 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000043 DAZKLA010000000
VERSION     DAZKLA010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.043"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..1019)
                     /locus_tag="ACG85N_001322"
     CDS             complement(132..1019)
                     /locus_tag="ACG85N_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFQ0224102.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            1204..2043
                     /locus_tag="ACG85N_001323"
     CDS             1204..2043
                     /locus_tag="ACG85N_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0224103.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2239..3213
                     /gene="sgrA"
                     /locus_tag="ACG85N_001324"
     CDS             2239..3213
                     /gene="sgrA"
                     /locus_tag="ACG85N_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFQ0224104.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            3511..3867
                     /locus_tag="ACG85N_001325"
     CDS             3511..3867
                     /locus_tag="ACG85N_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224105.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            3999..5027
                     /locus_tag="ACG85N_001326"
     CDS             3999..5027
                     /locus_tag="ACG85N_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224106.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(5053..5493)
                     /locus_tag="ACG85N_001327"
     CDS             complement(5053..5493)
                     /locus_tag="ACG85N_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0224107.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            5653..6159
                     /locus_tag="ACG85N_001328"
     CDS             5653..6159
                     /locus_tag="ACG85N_001328"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0224108.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            6327..8240
                     /locus_tag="ACG85N_001329"
     CDS             6327..8240
                     /locus_tag="ACG85N_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0224109.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            8245..9246
                     /locus_tag="ACG85N_001330"
     CDS             8245..9246
                     /locus_tag="ACG85N_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0224110.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(9448..9654)
                     /locus_tag="ACG85N_001331"
     CDS             complement(9448..9654)
                     /locus_tag="ACG85N_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFQ0224111.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(10300..11148)
                     /locus_tag="ACG85N_001332"
     CDS             complement(10300..11148)
                     /locus_tag="ACG85N_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0224112.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            11312..12214
                     /gene="gnd"
                     /locus_tag="ACG85N_001333"
     CDS             11312..12214
                     /gene="gnd"
                     /locus_tag="ACG85N_001333"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFQ0224113.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            12216..13217
                     /locus_tag="ACG85N_001334"
     CDS             12216..13217
                     /locus_tag="ACG85N_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFQ0224114.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            13214..13498
                     /locus_tag="ACG85N_001335"
     CDS             13214..13498
                     /locus_tag="ACG85N_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFQ0224115.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            13531..14328
                     /locus_tag="ACG85N_001336"
     CDS             13531..14328
                     /locus_tag="ACG85N_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0224116.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            14342..15175
                     /locus_tag="ACG85N_001337"
     CDS             14342..15175
                     /locus_tag="ACG85N_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0224117.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            15193..16050
                     /locus_tag="ACG85N_001338"
     CDS             15193..16050
                     /locus_tag="ACG85N_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0224118.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            16072..16581
                     /locus_tag="ACG85N_001339"
     CDS             16072..16581
                     /locus_tag="ACG85N_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0224119.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            16618..17019
                     /locus_tag="ACG85N_001340"
     CDS             16618..17019
                     /locus_tag="ACG85N_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224120.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            17131..17484
                     /locus_tag="ACG85N_001341"
     CDS             17131..17484
                     /locus_tag="ACG85N_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224121.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            17518..17961
                     /locus_tag="ACG85N_001342"
     CDS             17518..17961
                     /locus_tag="ACG85N_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0224122.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            17978..19063
                     /gene="uxuA"
                     /locus_tag="ACG85N_001343"
     CDS             17978..19063
                     /gene="uxuA"
                     /locus_tag="ACG85N_001343"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0224123.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            19056..19769
                     /locus_tag="ACG85N_001344"
     CDS             19056..19769
                     /locus_tag="ACG85N_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0224124.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(19976..20125)
                     /locus_tag="ACG85N_001345"
     CDS             complement(19976..20125)
                     /locus_tag="ACG85N_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224125.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            20521..20721
                     /locus_tag="ACG85N_001346"
     CDS             20521..20721
                     /locus_tag="ACG85N_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0224126.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7099 a         3204 c         4315 g         6139 t
ORIGIN      
        1 gatttgtttt ggtacaaata aaaaaaggcc ccgatgaagt tgtggcaaaa gtggtttgcc
       61 tcaaaaataa gaaggaattt ccaaaaatca cttctcaaat tttgccacat ccccaattgg
      121 gtcaaatttg tttatttgaa tatatttttt acataaggat ctttaatagt tacattgtct
      181 tttttcatca ctttacggat tgttttatcc atgaattccg tatcaagagt ttttgcttcg
      241 gtagcaattt tttctagctc agatttgtat ttgtctttat tagaaccttt atcctgtttt
      301 tttaccattt ttactagata atagctttgc tgataagtcg ttggatctgt tacaggaatg
      361 atatcagaaa cttccccgtt ttttagttta aatgctgctt tttttacttc agtaggaatc
      421 tctggattgg ttgaatcaaa attcattttt ccatcagttt cctttaatgt tttatttttt
      481 ccataagctt gaacaagctt ggagaaattt tctccttcat ttgcttcttt ttttgcttta
      541 tctgcttctt ctttagtcga aaatagaatg atctgtgtac taacttctgg atagaattct
      601 ttccaagcag tgtctagatc tttatctgtt aattttacat tggctttcaa accgtattgg
      661 aaagccagca tctgtttaaa gagtttctta tatgtctctt ctgtttggcc aactgctttt
      721 aattgttcct tgtagtttgg accgagtttt ttgatttgtt cattatattc ttttgttact
      781 tcctttgaag aaatctcttt tccgtataca tcatcaaatg ctttgaagac aatcattttt
      841 tttaatagtg tttgattatt tgatttaact tttgggttat cataaaaatc ctgaactgta
      901 agtgttccac ctttaaacgt tgcaatcgtt tcattggact tgctacaacc tgagaatagt
      961 aaaattgcac atgctgaaag gcacataatt agtagttgtt ttttcaaaaa atttcccatc
     1021 ctttccttat taaaaacagt ctaacagaag aattagggag acagtctttc attttcgtaa
     1081 ggttgctgta gatattctta gttcgtaaaa atctattctc tctatgatgg agaacattta
     1141 catagttttc acgagacaag aaagaaaagg gttgttaaac ttaatataaa ggagggatga
     1201 aagatgatca ggattattcg gttgattgga tctgttttaa tagtcttagt tggttttgtt
     1261 ttaggaattc ttgtcaataa tgcacttgcg gatatgttta ttggaaatag tgaatggatt
     1321 ggagcaattg ccggtacggt cggacattta gctgtttttt tactagcttt atttcttgct
     1381 agtaaggtcg aaggaaaaag aatagaagat tatggtgttt ctgttcgagg aaaagactgg
     1441 gggtatttag cgggaggtct cgttgttgga attggagtgt ttctgcttat tactagtcca
     1501 ttatatttga cgggggcgta tcggttagat tcaggaaacg caaacattgt gccgctgata
     1561 acgagtttta ttttatttat tgcagtgggg gcatcagaag agcttttatt ccgtggcttt
     1621 tttcagcatc agatgcttag atttggtccg ttaatagcaa tgattggatc tgctgcatta
     1681 tttgctcttt tgcacggatt gaatcctaac atgacatttc tggcagtttt caatatcttt
     1741 ttagcaggtt gctttttcag cgcacttatc tacagtacag gaagtttatt tacggctatt
     1801 ggtgctcata ttacttggaa ttggacacaa ggagctattt tgggtatacc agtcagcgga
     1861 acgcaagaac aaggtttttt ctccactctg atccaatcgc aaaatacaat aattactggg
     1921 ggaaactttg gggctgaagc atctatatca actacattat tcttgttagt cttgattgtt
     1981 ggtttgttac tctatgcata taaaacaaag cggatgaaga aatacactgc tgtttatcaa
     2041 taaaagatga agaaagcaag gctgatatcg gtcttgcttt tttaagtctg tcattgatta
     2101 aacgaaaaag taaacaaatt caagcgaata cacgacatct tttgttagat tttcacaact
     2161 gactgtcgtg ttctcatcat tttcatttta tataattcct ttatcaaata gaaggtaatt
     2221 atatggagag tgatagaaat gaagaaaaca gcgaccgtat tatttgttat tcttgggctg
     2281 agtataattg cagcaatgat tggaatcagt acttctgcaa atgaacgggc aaatgagaat
     2341 ctttcattta ctgtgaaaac tgatcggatt gtttatgaca tgacacaaca agtcattact
     2401 attccagtca aaccaaacaa aagcgtgaat gctagtgatg tacatgctgt tctaacatat
     2461 ggttgggatg gtaatggaag ttcagaaaaa gttattgggg aagtttattt aaaagacgta
     2521 caatggaccg ctggtattga gtatacaata atgatttctg cagaactatc gattgatgag
     2581 atcaagagta aagataaagt tgatttgatt gttttttatg atggacagat gacgatcacg
     2641 gagaatttga aaccaagcag ttggacagtt gttggaccgc caagcagtac ggatagcaca
     2701 agtagtgaaa gcagtacaga aaacacgagc ggtgaaagca gtacagagag tacgagcagt
     2761 gaaagtagca cagaaaacac gagcagcgaa agcagtacgg agagtacgag tagtgaaagt
     2821 agcacaggaa gcacgagcag cgaaagcagt acggagagta cgagtagtga aagtagcaca
     2881 ggaagcatga gtagtgagag tagcacagga agcacgagca gcgaaagcag cacaggaaaa
     2941 gtaaataatg aacttgattt ggtacctatc ataccaacta aggaacaaaa gagaaatgca
     3001 gggagcaacc taaaaagtgt gctaaaacag cctgattttg tttctcgtgg aattgatcat
     3061 caagaaaaag cgaaagaaac agataataga aaaagcgatt taccagaaac tggtagtaga
     3121 accttgaaca agctaattac atggatgggt gtgttactca ttttgattgt aggagcaagc
     3181 tattttcgtt ctctcttcca tcgagtgaaa taaagaataa aagcaacaga tctaaaattg
     3241 aataaaacga tagatttttg agtgaatatt tcattcgaga gtgctgtatt gcctggcgaa
     3301 gttgtagctt tgccagatct atttttgttt actaacatat ttgagaaaca ggaaataaca
     3361 ggaacacaaa tggaaaataa gagcaagaat aattgacaat gcacactgcg catggtagta
     3421 tctctgaaaa atcagtaacg aatctatttg tacgttattc atgtagctgt cggaataagt
     3481 ctaaataata gataaaaagg agtatgtact ttgtttgaag aatttattga tatcaatgaa
     3541 aggcaagtat atcaattttt gaactattgt tatgaaagag acgagaaatt atatgtggtg
     3601 aaggatatcg ctttagatct aaactacacc ctggctaaga tgaattcggt gattcaacaa
     3661 gcagaatcgt tttgcgaaag gtatccagaa tataaattat cttttctttc agaaaacaag
     3721 atgataaaag tcgagttttc ttctcagttt ttactgagta aagtttattc tatactttta
     3781 gagggaacga ttggttatat tttactggat tcactttaca aaggtactta tcaatcctta
     3841 gaaaatctta gtcaaaaaat tatataagtt tgcgtacgat tcaaagaaaa ttaagagaac
     3901 ttaatactat tttgaaaaac tatcatttat cttgcagctt gaaaaaaggt aatccgatag
     3961 aaggagaaga ataccgtatc cgatactttt ttcacttgat gtattggcag tcattcgacg
     4021 aaaatcattc tgatatatta tcaagtataa aaaataaacg tcaactatta gaaaaatttg
     4081 cgaaacatgc gccatatacg cgtagtatag atagaaagaa atggatcagc ctactaagaa
     4141 tttcgttgca acgaatgaaa caaggccact cgattacgta tgtcccagaa gagatgaaca
     4201 aatttgtgca tccattgata acaaaagaag agtttacgca ttacatacta gagccttttt
     4261 ttgaagcaaa cttctggtcg atgaatcaca tcactccaac agagcaagct tacctttatt
     4321 ttatgtttgg tgtaatgaac acctatttgg aagaagattt gttggatact ccagataaag
     4381 tacaagaaag ttggggctgg attgaatcgt ctattgtaga aaattttgaa aaaaacttca
     4441 agatttgttt tacaacttca gaaaaaaatt atctatatgt caatttggca atgatccatt
     4501 tgtattcaag ggtttttggg acaaaaaaga aggtggatgc ttttggaaaa tcagcagaag
     4561 acagagattt tcagcatata tttccagtga tgtatccaat catgcaacag ttttttagtg
     4621 gggtagctcg cagaattcca gagcttaata cttattatga aaagaacaga cggttgattt
     4681 ttcaatactg tatgttagtc agggatattt ttataaaaca cgaacaacct gtcatattct
     4741 atattcaatc aaagtatggt aaaacacagg aattatgggc aaagaaaagg atattatcta
     4801 tgactgaacg cccaataaaa tatagtgcat ccgctgatca gttacctgat attgtcatta
     4861 gcgattatcc tatcgacaaa aaggcatatg atcaaacgaa tacacagata ttttactgga
     4921 atggacaacc tacaaaaaat gattgggcgc gtctgaaagc tgaaatcata aaaatcagga
     4981 atgagaaaaa tagatccata ttggaaaaat tggatttcgt taattaagaa aagcagattg
     5041 tttatgccaa aattaagaca ttgcaaaaag attattttct ttaaaatcga caagatgacc
     5101 agaaagcaag agttcgttga gctcttgctt tcttttagtt ttagaaagga acatcctgat
     5161 tttatcttcg ctaaatccag tcaaaagttt tgcagattgg tcgtagataa tcggtttttt
     5221 caataaagaa ggggatgtac gaatctcaat atatagttca ctcaaactga gttcatctat
     5281 tctgtgaaca agatttttat aactaataga atgagtagag agcatttcgg tcacgccgtt
     5341 gtttgtcaat tttagtaagt ggataaattc atttttggtt agtggttgac gaattaagtt
     5401 ctgttcgata aacggtatat catggacgat aagccaacgt tttgcgcgtc ttgaagacga
     5461 gcagccagaa cctgtataca atttaatcat catacattcc tccaatagct aagtatttat
     5521 gaaataataa catgaagaaa tagtcgaaaa gtgccattta gaggtgtaaa ataagaaaat
     5581 gttgtcacgt ataatggaac tagctagata agaaatacta ttaaatcaag aaaaaagaaa
     5641 gaaggcacaa gtatgtcaaa cacagccgac gcaatagtag tgatcgattt acaaaaaggg
     5701 gtatgttttg attcttcgcc gatacacgaa ttagaaacag tgacagcatt agtgaataaa
     5761 agaatcaaaa catacgaaac gattgggaaa ccagttattt tcgttcaaca cgaagatgct
     5821 caattagtgc cacaaacaaa accatgggag ctattgtcgg aattatctat tcctgagcac
     5881 agttattttg tgagaaaaac ccatgcgaat tcattttata agactaattt gagtgtttta
     5941 ttaactgaat tggactgtca ttcgatcgaa ttatgtgggg cacaagcaga gtattgtata
     6001 gatacgactg tgaagtttgc tcatggatta ggataccagc ttatgatgtg tagaggagca
     6061 acgacaactt atgataatcc ttttatgaaa gcaaaggaaa caatcgcttt ttacgagaaa
     6121 atttgggata aacgatttct gacatttttg gagcaataag ttgtttcttc gtttaaaaaa
     6181 agcagaatgg atactggttt atccattctg ctttattacg taaaatgaga aaataatcct
     6241 gttctatttt tatgggataa accatttatt ttttgtataa aaaatgtttg cgtttacata
     6301 taattctcct agaaacataa aaaggagtga atagcttgag aacaaaacaa gtattaaagg
     6361 gagcgttggt catcttattt gctgtaagta caagcgtccc gcaatggaca gtatatggag
     6421 agagcatttc ttccgaatcg gaatctcggg aggtagaaac cacagaaact actgatgctg
     6481 gaaatgaaac gaaatcgagt acttcacaaa cagaaacaaa tactgaaaca acggttgaga
     6541 catctgttac tagcacagat caagaaacga ccgctaccac agataaccaa caaacagaag
     6601 aagcaacagc tacaaaaatg tcaaacgacg aatcctcttc tgctaaagta agtgacaaag
     6661 agaatgggac agaaaatgat gatatcggta aaggtgaaaa tttcaatggt aaagaatatt
     6721 tgaattactt cggtaaacag atgacagaaa tacttaatgg accaacagaa gacatcaagt
     6781 caaaattaat agagttatta gtcactgagc ttgctgttaa tgattatggt gtagataaag
     6841 agattatcaa acatctgatt gctacttatc tattgtacga tatcgctttt gcaaaaaaca
     6901 gtgtgttgat gtcgatgctt ggaggtacag atgatgggac aacgcctaca gaaaacacag
     6961 gcttctatcc aaatacaaaa caacttcatc aaactgtact aaacaaacaa gatggcaaaa
     7021 tgatggatgt atatgcatat tatgtcgatc aaggttcaga taaaacagtc atgatccatg
     7081 gcggatttcg agggaattgg aataacggga tcgttacaga agaatacaat gatttttata
     7141 aggccgggta taatttatta tttgttgatt ccagagcgac tggtaatagc ggaggcgatt
     7201 atgtgaccta tggacaatat gagtcagatg acgtccttta ctggataaat caagaagttc
     7261 gagagcggcc atctcaaaag attttacttt atggtggttc gatgggagca gctacgatga
     7321 tgtctgtatt agcgaaagat attcctgtca atgtcaaagg aattattgaa aattgcggat
     7381 ttgcatcaat tgatgaacaa ctacgtttta cgtacagcca gacagtagcc cccgcactac
     7441 cggatgccat aaaaaatcag ttagacatca ttggggatca agagcatgaa gatttattta
     7501 tgggcctttt gaaacaatac tattttgatc aggagatgca tttggataca aaagctgctc
     7561 tacctacgat tggaatgtca gattcattgc ctaaattgat tatccatgga acagcagatg
     7621 atgtagtacc tgtgtctaac gcgcaaaaat tatatgagtt atcaggaggg tataaagatc
     7681 ttttattagt agaaggagca gggcatggaa aggcacagga agttgaccat gctgcctaca
     7741 cgaaacatgt aacggacttt ttgaaagtag tgttccatga tcagataaac gtaaagtatg
     7801 tggatgaaaa caataaaagt ctactcaaag accaggacga aatccagctt tacggagctt
     7861 atggagaaaa ttatatgaca gagcaaaaga cattcgaagg atacgaatta gcaaatgttg
     7921 aaggaccgac tgaagggatt tttaatgaga cgataccaac tattattttc tattacaaaa
     7981 aaatcccagt agctccacca aaaaagcaag atccgacaga gaatgcagac aataagggaa
     8041 agaatgaacc tcgagaggat caatcaagtg atgatacgca accgattcag atgacagtga
     8101 aagtacaaaa aaagacagat gcaaaaaaaa gaatgttgcc gaaaactgga gaaaagaaac
     8161 attcgcttct catttattca ggtggatttt tatctgttag cacgattatc acatggattt
     8221 ggcgaagaaa gagagggtaa atgaatgaaa tggttcaagc gaatagtgat tgcagtacca
     8281 gttatcatcg tagttatttt agtcactgta tttttgatta atcaaatcac accaaagccg
     8341 attagcttgc ttgttagaaa acaatttgac acaagtgaaa aagaacaagt atatgcccga
     8401 ccagatgatt tccagcaaaa aatcgaacaa atcgatataa aaaaagaaat atcctatcct
     8461 tcaaagtaca aaaataataa tatggatatc tacacaccga aagtggaaaa gaaaaaatta
     8521 ccgattcttt tctggatgca tggaggcgca tatgtaggag gggataaaaa tgattgtcgt
     8581 gattatttgg aatatctatg ttcagacacg cagcaaatca tcgtaaatat agattatgaa
     8641 cgatcaccgg aagcaaaaca tcctacacca gttatccaat tgaacgaagc cattcaagca
     8701 gctaaaaaag agataaaaga tcaagcagat tggtcgaata tttcgattgg tggaaattcg
     8761 gcaggtgcac aaattagtgg agaatatttg ttagcacttc aaaatgaaga aattaaaaaa
     8821 gctgatcatc ttgatccgac tttggagaac aagcagatta cgaagtttat ttctttgtct
     8881 ggtctgttgg atccttctgc acttactcaa gtaagtgata aaatctctag ctttctttat
     8941 gaaaaatgtg gatgggctta ctttgataat aaggactttg aacaatcaga gaaggtcaaa
     9001 agtttagcct tggtcagaca tgcggatcgt tggacgcaaa acacattctt aacggatgga
     9061 aatacaaata cgtttacgaa acaaatggaa gaagtaactt ctgtatttga gaaagcgggg
     9121 gtgaaagtca caaaagtaga ttatgcggag aaaaatgctg ttttgaacca tgaatatcag
     9181 tttgatttca gcaataaaca agcgcaagaa acatatcaaa agttagtaat gtttttcaag
     9241 gagtaaagtc ttatgatgca aaagaggttg taatctgtca cttagctcca agtcacaagg
     9301 agcaatttca ataaacaagc ttttcatggt ttatgaatat tgtaacttgt gactgaggag
     9361 ttagagatgg caaggggcac cttttattcg aaatttggga atatcacacg attattgtca
     9421 cgatctcttt tacgtaaatt ttataagtca gatttgttgt tttgatcgtt taaaaaaagc
     9481 aacaaatccg aagcctgcgg aggcaagaag ggacaaaaag ccgagtggta agcaaaagaa
     9541 tgctggttcc actaatattc cgttgttatt gatataaggc tgggaacaaa gatgaatagc
     9601 aaataaaatc aatgcactag cgaaaaaaag caaagaaata tggatagctt tcataagcag
     9661 cacctccttt tttataattg tatgataaat agattaagaa tctataaatg tgacaagttt
     9721 gttaggatgt gaataaaatg atcatctagc gaacttgaaa caaagatggc taatgataag
     9781 caattaatga ttcagcaatc agataggtac aaagtttacc atcatacgtc tagaccaata
     9841 atagaaaata tgggaatata atagaaaaga tttaggatac ccccaaaaga atttccccaa
     9901 aaattcgatg tatcctaaac ttttttattt tacataacaa tcatatcaac tgtgaaataa
     9961 aattacaaat gataacctaa aatgaggcca aaagccagtg ttatcaagga aatacttaat
    10021 tcaccaaaat tttttttaat tttttagaaa ataagaaaca aaaataataa aaaaaagaaa
    10081 agataaattt tttactactg tatactaact ttgttatcgc atctaacaag taactcgaat
    10141 aataccaata caaccttctc caaagatgcg atgacatacg ttctaaccca ccgactattg
    10201 atgttctgta ccacccttgc agaaccaaaa aagccactac caaatatttg gtagtggctt
    10261 tttttgtttt atggattttt cgctttttct gttatggtgc tattctttga tttcagaaat
    10321 agtatgtcga actttagcga tagaacgacg tgattctttt tcgttgtaaa acatcaaaat
    10381 cacatataga gagtccaaaa tgcttagttg agaaatacgg gaagctaaag cttcagatcg
    10441 gaattctgtt tcttcagaaa tagaaataaa aacgacatcg ccaagtttag caagggaaga
    10501 attagcttgg ctagtaataa cgattgtttt tgctccagtt tttcttacgg tttcagcaat
    10561 atggattgtt tcttttgatt gccctgtgtg agaaatcaaa acagcacagt ctttttccgt
    10621 caataaagaa gcttccatca attgaatatg gtaatccgtg ctgtgagaag ttgcgatagg
    10681 ggaacgcaaa aatttatgat aagcgtcagt tgctacaatc tctgaaccgc caacaccgaa
    10741 aaaatagaca cgtttagatt cattgataat agtagccgct ttttttagat ccttttgttg
    10801 taacaacttt tttgtgtcat ttaaagttga aatactagac tcgaacacct tttgcgccat
    10861 agtgagctca gtatctgttt tctgaatatt ttcgtggata gaaatagcag atagatcatt
    10921 ttcttggatc aacatggcca ttttaaaatc tttaaatcca ttataaccaa gtttttttgt
    10981 aaattgaaaa aaagtagagt cagcaatgcc cagttcactt gctaaattgc taataggact
    11041 atgacttact ttgcttggat tttctaaaat ataatcggcg atagcctgtt cttttgggct
    11101 gaagtcgttg tacattgttt tgatttgtag tcttatggag acgctcatga gaattcctca
    11161 ttttcataat aagattatca ttttttccgt tctcattaat gttaaagata caaggagagt
    11221 aggaaaaaat caaacaaaaa atatttttgt aaaatctatt tacaaaaaaa tttttttgta
    11281 taatgagggt ataagaaatg gaggcgctat catggaaatt ggtgtaattg gtttgggaaa
    11341 aatgggattg aatttagctt taaacttaaa agagcaccat cacaaagtac aaggactcga
    11401 catttcaagt acagctgtta gtcaggcaag agaaaatgac attcctgctt atcaagagat
    11461 ctctgaattt ctagctactt ttgagcagaa aaaaatcatc tggttaatgc ttcctgctgg
    11521 agagataacc gaaaacatgt taagaaaact ttttccaaaa ctcggaccag gagacattat
    11581 tattgaaggc ggaaactctt attataaaga ttcaatccgt cgagcagaag agtttcagaa
    11641 aaatgaaatc ggttacttgg attgtgggac ttctggagga atcgaaggag cacgacatca
    11701 agcctgctta atgattggag gagataaaga aacttttcta tccgtggaac aattattcaa
    11761 agatgtagca attgaaagag gatatctgta tgctggtgca tccggaagcg gacacttctt
    11821 gaaaatgatc cataatggaa tcgaatatgg aatgatgcaa gcaatcggtg aaggattcca
    11881 acttgtacaa aaaagtgagt atgcttttga cttggaacaa gtcgcacgtg tttggaatca
    11941 tggttcggtg attcgcggct ggctgatgga aatcatggaa gatcagttaa acgaacatcc
    12001 caatttaagt aattatcggg gaatcgtttc tgcatcaggt gaagcaaaat ggacggttga
    12061 aacagcgcta gaaatggctg ttccagttcc agtaattgca actagtttgt tcatgaggaa
    12121 tctttcacag gaagaagata gtttttcagc aaaagtagtt tcagcactaa gaaatgggtt
    12181 tggcggacat gaaatagttg aaaagggtga gtaaaatgcc agattatcat atagcaatcg
    12241 ttaattccag cagtttcgga agagtctttc cacaccactt ggaaagatta aagtcagttg
    12301 gagacgttca tttctttact gttgatcaag agattccagg aaaagaacta gcggagttac
    12361 ttaaaggatt caatatcatt attgcaagtg ttacgccatt ttttcgacga gatttttttg
    12421 aaaacaaaga tgaactgcta ttgatttccc gacatggtat tggttacaac aatattgatt
    12481 taaaagcagc aaaagaacac cagacaatcg tatctatcat acctgcttta gttgaacgaa
    12541 atgctgtagc agaaaataat ataactaatt tgttgtccat gcttcgacgc acgacacaag
    12601 cacaagcaag agtcaaggac gatcgatggg aagatcgggc ggagttcgtc ggtaggacgc
    12661 tgtttaacaa aacagttggt gtgattggtg caggaaatac cggaagcgga gtaatcgaaa
    12721 ttttgcgtaa cggttttcgc tgtgatgttc tagcttatga tccttacaaa tcagatttat
    12781 acatcgaaag ctttggtgca aaaaaagtag aacttcaaga attactggaa caatcagata
    12841 tcatttgttt atgtgcgaat ttgacagaag agaactatca catgatttct gataaagaag
    12901 tcgatcagat gaaagaaaat gtttatctat ctaatagtgc aagaggcgct ctagttgatg
    12961 aagaagcgat gattaatggt ctgaaaagcg gaaagatcgc aggatatgcc acagatgttt
    13021 tagaagaaga accgggacgt caagatcatc cgtatttagc gtttgaacaa gtagtgatta
    13081 cgccacatat atcggcctac acaagggaat gtttagaagc aatgggtgaa aaatgtgtca
    13141 gagatgtcga acagatcagt cagcaccttc ttcctgaacg ttctgtccag acaaagagca
    13201 actatataaa aggatgatga agaaaatggt gaacggttta aaagtgaaaa caggccctca
    13261 attttattta tatgaagaag gtggtatttc aaaagtttct gatctcttga aatcatacgg
    13321 cgcgaaaaga gtactggtca cccacggaac ggtatcatgg gaaaaggcgc tacctaaatt
    13381 agtatttttg aatgacgaaa cgattcaatt tttttatcat cgttattctg gagaatgtag
    13441 ttatgctgaa gccagacgaa tagctacaat catcaaaaaa atgaaatcga tttcgtagtt
    13501 ggcgttggcg gaggaaaact gaccgatcta gtgggttata gtgccaatct tgcaagaatc
    13561 gatttcggag tcatccctac tttagctagt aattgtgctc catggacacc tcttgctgtc
    13621 atgtataagg agaatggaca agcagaaggg aaaacagaac acttttacag acaagctgct
    13681 ttttttataa cagatcccca attagtgatt gattcccctg ttccatattt cattgccggg
    13741 ttagcagata cactagcaaa atggtatgaa tctgagctca tcttagaaca agattttttg
    13801 caagatgaat catttttgaa gctagcacaa gatactgcga agatctgcaa agaagagatt
    13861 ttgaatcatt ctgctaaagc aatccaagat atggcacaaa gaaaactgac atcggagttc
    13921 aaacagcttt cagagattat ctttgctgtt gctggtttag ttggaggatt aggagataaa
    13981 tacgcaagaa atgtggcagc acatgctatg catgacgcta tcagtaaata tattccggaa
    14041 agccatcgtt ttctacatgg agaaaaagta gcgtatggga ttttctatca actggcacta
    14101 gaaggtcgct gggaaaaaat agacgagtta tatcctttgt atcgggaatt agaactgccg
    14161 gtttctctag aacaaatggg attatttccg aaagataaac agataataga taaaatggct
    14221 gaatttattg attcgaaaga gaaagtgcat ttgattccta tagaggtaac acagaaatca
    14281 ctgatacagt caatgatgga actggaaggt tatatcagaa agatctgaag gaggaggaca
    14341 catggaaaca ttaagtgtag tacaaagttt gttgattgct ttatgggtag ccgcggttat
    14401 gtcccgctgg ttaggtggag gagccacgtt gaccttacgt ttttctcctt tgatgactgg
    14461 attagtcgtc gggttgatca tgggggaagt tgcacaagcg atgattgtta cagccgcttt
    14521 gcaaatgatt tatatggggg ttttttctcc aggaggatca atgccagctg aaccatctat
    14581 tgcagcagct atcgcagtac cagtcgcatt attaggggat ctgtcgccag aagcagcgat
    14641 tgccgttgcg gtgccagtcg gattgctcgg aagttacttg tatcagtttc gttttttcat
    14701 caatactttt ttaggtaaat atacagacaa agctgtagaa gatttgaata caaaagctat
    14761 ggcccgatca attattcttt atccaacggt tgcctcattt ttattattcg ttccgcttgt
    14821 tttcattgct ttatatttag gggcgccatt gatcgctgat gtaatcaaag ctttagaggg
    14881 aacagtcgtt atccacattc ttgaagtagt aggtggaggg ttagcggcaa tcggtatcgc
    14941 gacaactgta tacgttatcg gtcgaaaaga ttatctggtc tttttcttcc ttgcttattt
    15001 tatgagtgtt gttttgaaat ctttagatat tacgatggtt acttatgcca tttttgggat
    15061 tattatcgct ttgatctttg tccagtcgca aaaagggaaa caagtacagc ctgccacaag
    15121 caataacaat tcagcatctt ttgatgacga agatgactat gatgacggat tttaatttag
    15181 gaggaatggg aaatggctga aataatggat aatcaaacag gtgctcctga agaaatcacg
    15241 aaaaaagatg taacaaaagc ctatctgaga tggcattttg caaacgaaat tcctcactct
    15301 tttgaacgtt acttggcgcc atcactacta tacggaatga tgccaatctt gaaaaaactt
    15361 tataaagatg aagactcttt aaaagcagca tataaacggc agttactgtt cttcaacact
    15421 caattaagct ggggtggcgg tgtgattact ggcctaatgt cttctatgga acagcaaaga
    15481 gcagaagaag agtatgaaga aaaagagatc ttgatgcagg atgacttgat gtataacacg
    15541 aaagcgggat tgatgggggc actagcagga atcggagatg caatcgattc aggaaccgtc
    15601 caatatattt ttatcgcaat cgcggtacct tgggcacaac agggaagtcc tttaggcgca
    15661 ctttttcctt ttatctgctt tgggttgtat caggttttac taggggtatt tttcgcaaga
    15721 caagctttca ctatgggccg taatgctaca ggcctaaagc aaaacactgg tgttcagaca
    15781 gcgattgaaa tgctatcagt tttagggttg tttatgatgg gggtgcttgc gggaaactat
    15841 gtaaaagtag aatcttcatt gaaatttgcg atatccggac gtgagtttgt tatccaggaa
    15901 attcttgatc aaatcgtgcc agggatatta cctttagctg tcgtaatggg tgtgtacttg
    15961 ttctatacga aaaaaggatt gaaagtcaca caagcattat tatggctgac tggaatatta
    16021 attgtgctag caggaatcgg tattttataa aaagaaggga acgtgaataa gatgggtgta
    16081 gtcaatttag cgcgtgtgga tgagcgtttg atccatgggc aagtaatggt aacgttatct
    16141 caaaaaagtg gagtgaattc catttttgtt gtagacgaag ttgtagcaaa agataaattt
    16201 atgagagatc tttacaaaaa tgcaggaagc cgtacaggac aaaaaacaat tgtcattaca
    16261 ccagagaaag ctaaattcta ttgggatgaa tatcaattta aagaatacaa ttgtattttg
    16321 atcgcaaaaa cagtccaaac gatttacgac ttagtaaaac acggaatccc catggaagaa
    16381 ttgaatattg gagggattgc tcaaaaaaat cctgatacag atcttttagt gacgaaatct
    16441 gtttatttaa ataaatcaga tgctgaaaaa ttgaaagaaa tgaatgaaca atatggcgta
    16501 aagaatattt attttcaagc cactccatct gcacctaaaa caggattaaa agatgtattg
    16561 aacaagtttg atttgtcata aacaaatgct tagaaaaaag ataaagaggg cgaaaaaatg
    16621 aagaagaacg agctgtttcg tgattgggaa tttcgttatc gctatattta ccgtaagcgc
    16681 cgtacaaaaa agagtaaaca gcgttttctt tcagcattag tatcagatat ttacagtatg
    16741 agaacagatg tgacagtcat tgcttacgat acgctagcct accgatcaaa aaatatttat
    16801 gttggagata tcgaaaaagc agagaaagtt atctgtactt attacgatac acccgttcat
    16861 gcattaggaa gttatttcat gtttgattgg aaagaccaaa ggaaaaaaac aatctactct
    16921 atattattgt ccttcatttt gttgttcagt ttaggttggt ggggaatgat gatatataac
    16981 aaaaatcctc atcacgtatt cgatctgcta tccgtataaa caggtatcac tgtactagca
    17041 tttgggagct actttttctt attgggaaaa gcagctcgcg gatggcctag cagacaaact
    17101 tttattcgaa atacttcatc catattaacg atgctagaaa tgatccgtac gattgatgat
    17161 ccgagtgtag cgtatgcgtt tgttgatgaa ggctgttatg gagaaaaggg cttagacagc
    17221 gtgagatcgg gtatgaaaaa agaggctatt cttttttatc tagactctgt tggggcagat
    17281 acaccgctac agttttcggg aaattatttt tcaaataaag aacagtggct gaaacaagtt
    17341 gataagttga aagaaaaaaa cgtcaattat atttttagtg caagaaaaaa gcaagcgcag
    17401 ttcttctatt tgaccaaaac agatttacga gggaaaacat ttaattggca aaatgccaat
    17461 cagattattg ctctttttcg ataagtaaaa agtgcaaaac aaggagtgag aagactaatg
    17521 ttggaaatca tgatcgttac acatggtaag ttgagcgaag gactgaaaga tgcggcggaa
    17581 gtaattgtcg ggacatcgag ccatattgaa acaatgtgct tagagtctgg ggaggacatc
    17641 caaacattcg gagagaaggt caaagaaaaa ttaactatga tcaacacagc agaacccatg
    17701 ctagtatttg ttgatctaat tagcgcaagt ccgtacaatc aagcgctctt ggcagtcaat
    17761 gaactgactg aagaaaagaa agcagccact tatgtcatag ggggcgtgaa tctgccaatg
    17821 ttgttagaag caatcaatca tcagatgctt cacacaccga tagaagaagc ggtggaacaa
    17881 attgtcaaac aaggaaaaga taatttaact gtttggcatg cttcagcgga agaaaaaaaa
    17941 ttagatgagg acgattttta gaaggaaggc tgaaaagatg aagtggggat ttagatggta
    18001 tggtgaaaaa ggggattcga tcccattaaa atatgttcgc caaataccgg gaataaatgg
    18061 gataattgga actttgctga ctaaaatgcc tggagatgtt tgggagattt cagaaattga
    18121 ggagttgaaa gcatctgttg agagccaagg actggaactg cttggaattg aaagtgtagc
    18181 gatccacgat gcaatcaagg cagggacaga tgaacgagat tactatattg ataactatat
    18241 acgaacaatc aaaaacttgt ctcgatgtgg ggttcgattg atttgttata gcttcaaacc
    18301 aatttttgga tgggcgaaaa ctgatttgtt taatgaaaac aacgatggaa gctattctct
    18361 tttatacgat cagaaaatcg tcgatcagat ggagccagga gaaatgtatc aattgataca
    18421 cagccagtca aagggattca aattgccggg ttgggaagaa gaacggttga aaaaattcgc
    18481 tcagctggaa aaactgtatg aaggaatgac cgaagaagaa ttatttgaaa atctgactta
    18541 ttttttagaa aaagtgattc cagtgtgcga aaaagcagat gtaaaaatgg ctatccatcc
    18601 agatgatccg ccatgggaac tttttggttt tcctagagtg acgaaaaatc ttggcgactt
    18661 gaagaaaatt ctaaatgcag tagattctcc atataatgga ctaacgctgt gcacaggatc
    18721 tttaggggca aatccggaaa acgatttagt tgaaatcatc catgaggtag gttcgcgcat
    18781 ccattttgtc cacttcagaa acgtggagtt tttaggagaa agaaagttca aagaaacggc
    18841 acatccctct acagaaggct cccttgatat gtatcatatt atgaaagcgt tggtgcaaga
    18901 agggttcgac ggtcctatac gccctgatca tggtagaacc gtgtggggtg aagtggctat
    18961 gcctggatat ggcctgtatg atcgtgcaat gggactcact tacatgcaag gtctatatga
    19021 ggcgataagt aaggaaaaac cactgggaga gcattatgaa taaaatcaag ctcttcatct
    19081 ttgatatgga tggcctttta ttagaaactg gacgtttagc ttatcgtgct tatgtaaaat
    19141 cagcacaaaa gtatgattat gaaatgcgta aggaagtcta ttatttacta actggccaaa
    19201 cagaaatggc gataagagaa caaatgggca ttttgtacgg agaagatgtt ccatatatca
    19261 aatggcgaga agcaatcaat caatataaag aaaaaatcgt gaaagaagat aagagagtat
    19321 atacgaaaaa aggagcagaa gaaattttat cttttgcaaa agaaagagga atccatacaa
    19381 tcgtggcttc gtctaataca cgggtaaaag tagaaatgta tttaaaaatg gaaaatctat
    19441 atgccttgtt tgatcaaatc atttcagggg atgatgtaaa aaaaggaaag ccggaaccag
    19501 agatcttttt gaaagcttgt tcaaaaatga atattcctcc aagtgaagca cttgtatttg
    19561 aagactcgat tgcagggatc gaagcagcaa ggcgagcggg cattttgtct tttctcatca
    19621 aagatcattt gatagggttg cccgatcata atgggaagca taagttgaaa gtagatgtaa
    19681 gtttatatga atccaataaa gcagactatg aattttatga cctgcatcaa gcaaaacgat
    19741 ttttagaaag taaagggttg tatctctaat aaagaggact attttctagt cctctttatt
    19801 tattgtcctg cacgactgag ggaacagatg aaaaagaagt aattttttat tttgaagtat
    19861 ctaccttatt tgtagtgaac aatttagaac aaacacaaaa cgcatctact gttatctacc
    19921 agtagatgcg tttaaagaga agaacgagaa tcgtccatat gagggtttcc actccttatg
    19981 cagatttttt taattcttgc actgcttgtt cgatcgtaac acctgtcgct acatgattgg
    20041 tattttcttt gtcaaaaacg acaaatagat tcaaatctgt atccaaggtt actcgatatt
    20101 gtatatcttt gttatagaat gtcatagcca ttctcctttc tttaaatact tgctgaaagg
    20161 caacgaacgc gttgataacg aaattccatt tataagtata ccaccaattg aaaggttttc
    20221 atagttaaga aattttaaag tcaaaagttg cagataaaag caaataaaca agaaaacaaa
    20281 aagtgaatca atcgcggtta ttcaactttt tcttcgattc gaagacaata gaagacaatg
    20341 aaatttatat ttcattgcat tatccttgtg ttattagaaa aagcatgata tattaaatga
    20401 gtaatctatt ttgaaacgta atttgagcga tatatctaca tagataaaac tccttttgcc
    20461 tatcaaatat ttattgcaac gaaaaaacgt acattgatac gtatcaggag gaatttattt
    20521 atgaataacg gtacagtaaa atggttcaac gcagacaaag gttttggctt tatcactggt
    20581 gaagatggaa atgatgtatt tgcacatttt tcagctatcc aaggtgacgg cttcaaaact
    20641 ttagaagaag gccaagcagt gtcttacgat gttgaagatg gtcaacgtgg ccctcaagca
    20701 acaaacattg ttaaagctta ataaaaattg acttttaacc gtcttttcga aagaaaa
//
LOCUS       DAZKLA010000044        20362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000044 DAZKLA010000000
VERSION     DAZKLA010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.044"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            291..1430
                     /locus_tag="ACG85N_001347"
     CDS             291..1430
                     /locus_tag="ACG85N_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFQ0224127.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="ACG85N_001348"
     CDS             complement(1497..2072)
                     /locus_tag="ACG85N_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0224128.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="ACG85N_001349"
     CDS             complement(2077..3051)
                     /locus_tag="ACG85N_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFQ0224129.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="ACG85N_001350"
     CDS             3334..4302
                     /locus_tag="ACG85N_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0224130.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="ACG85N_001351"
     CDS             4415..4693
                     /locus_tag="ACG85N_001351"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0224131.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="ACG85N_001352"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="ACG85N_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFQ0224132.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5150..5905
                     /locus_tag="ACG85N_001353"
     CDS             5150..5905
                     /locus_tag="ACG85N_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0224133.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="ACG85N_001354"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="ACG85N_001354"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFQ0224134.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="ACG85N_001355"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="ACG85N_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFQ0224135.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="ACG85N_001356"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="ACG85N_001356"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFQ0224136.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="ACG85N_001357"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="ACG85N_001357"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFQ0224137.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="ACG85N_001358"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="ACG85N_001358"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFQ0224138.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="ACG85N_001359"
     CDS             10642..11184
                     /locus_tag="ACG85N_001359"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFQ0224139.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11207..12763
                     /gene="atpA"
                     /locus_tag="ACG85N_001360"
     CDS             11207..12763
                     /gene="atpA"
                     /locus_tag="ACG85N_001360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFQ0224140.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKFVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="ACG85N_001361"
     CDS             12777..13679
                     /locus_tag="ACG85N_001361"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFQ0224141.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="ACG85N_001362"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="ACG85N_001362"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFQ0224142.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="ACG85N_001363"
     CDS             15122..15544
                     /locus_tag="ACG85N_001363"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFQ0224143.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16013..16243
                     /locus_tag="ACG85N_001364"
     CDS             16013..16243
                     /locus_tag="ACG85N_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFQ0224144.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16340..17641
                     /gene="murA"
                     /locus_tag="ACG85N_001365"
     CDS             16340..17641
                     /gene="murA"
                     /locus_tag="ACG85N_001365"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0224145.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17654..17830
                     /locus_tag="ACG85N_001366"
     CDS             17654..17830
                     /locus_tag="ACG85N_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0224146.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17965..18261
                     /gene="yidD"
                     /locus_tag="ACG85N_001367"
     CDS             17965..18261
                     /gene="yidD"
                     /locus_tag="ACG85N_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFQ0224147.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18325..18708
                     /locus_tag="ACG85N_001368"
     CDS             18325..18708
                     /locus_tag="ACG85N_001368"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0224148.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18877..19761
                     /locus_tag="ACG85N_001369"
     CDS             18877..19761
                     /locus_tag="ACG85N_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0224149.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19875..20111
                     /locus_tag="ACG85N_001370"
     CDS             19875..20111
                     /locus_tag="ACG85N_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFQ0224150.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20199..20279
                     /locus_tag="ACG85N_001371"
     tRNA            20199..20279
                     /locus_tag="ACG85N_001371"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20233..20235,aa:Tyr,seq:gta)
     gene            20286..20357
                     /locus_tag="ACG85N_001372"
     tRNA            20286..20357
                     /locus_tag="ACG85N_001372"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20318..20320,aa:Gln,seq:ttg)
BASE COUNT         6760 a         3566 c         4425 g         5611 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatttg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat acattttatg
    15961 gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt
    16021 atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt
    16081 ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat
    16141 tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt
    16201 agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac
    16261 tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga
    16321 attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg
    16381 aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt
    16441 agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat
    16501 gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt
    16561 aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat
    16621 gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc
    16681 tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca
    16741 agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt
    16801 gatcggtaac acgatttatt tagatttccc tagtgtgggt gctactcaaa atatcatgat
    16861 ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat
    16921 cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga
    16981 aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga
    17041 tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat
    17101 tgcagatgct atctctgaac ataactgccc attgatttct aaattgatcg aaatgggtgc
    17161 agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac
    17221 agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc
    17281 tatccaatta gttgcagaag gtacaagtgt ggtgactgaa acagtgtttg aaaatcgttt
    17341 ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc
    17401 aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc
    17461 agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt
    17521 gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga
    17581 cgtcgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta
    17641 aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa agattttagt
    17701 ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat
    17761 cggtggcggc aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt
    17821 ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca
    17881 gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt
    17941 aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat
    18001 cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat
    18061 tatatgataa aagcaattca aacccatggt gcgctaaaag ggacggtaat ggggactgca
    18121 cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc
    18181 cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat
    18241 acgggtagca ctgagcgata aagaaaaaga ggaaatttat tgtcaaagta acaactccgc
    18301 aactacttat atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg
    18361 tccttgttat tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg
    18421 agtggctgga tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg
    18481 gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa cgtatcaagg aaaggggatt
    18541 ggcggaaaat tacttgagac agcttttcag aagctttcac agctagcttc aaaccaaact
    18601 cggatcatta tacgtactgg cactactagt agcgctgcgt tacacctcta tcaaaaaatg
    18661 ggctatgtgg agattggtcg agtcaaagac tattttatcc atcattaatc tgagcccatt
    18721 tttgaaaatg gagatcaatt aagggatcaa gtgattttag caattcactt acctaaaaat
    18781 agctttaact aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt
    18841 acaatagctt tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt
    18901 ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa
    18961 tttgcaacgc ctgaatcttg ggaagaaatg aaaacgcaat tatttcaagt ttataaaaaa
    19021 atactcgttt cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat
    19081 gagaaacagg tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc
    19141 cgagatttgg aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc
    19201 ggaatggctg aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg
    19261 gcaggaactg ggattggcgg ggcgttgttc acgaaaggac aaataaataa aggggcacat
    19321 ctttacggtg gagaatttgg gttgatgttc ttagatggcg ataagacatt cagtaaactt
    19381 ggtacagcgg tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaatgct
    19441 tataccggaa aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa
    19501 gttgagagtt tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat
    19561 ccagaagtga tcgtattagg cggcggggtt tctgcaaaag aagggctcat tgatgagatc
    19621 aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt
    19681 ttattatgtg agtacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct
    19741 gtacgtcaag gagagaatta agagctttag agaaggtgca gcttgcataa aagaagagaa
    19801 tgaaatgaat aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata
    19861 tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat tgaagtgcgt atagaagaag
    19921 tgaaaaaaga tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa
    19981 tgattggtga gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag
    20041 attttggaaa agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc
    20101 tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat
    20161 taggtattat aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa
    20221 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
    20281 ttcattgggg tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc
    20341 gaatccagct accccagtat ac
//
LOCUS       DAZKLA010000045        19988 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000045 DAZKLA010000000
VERSION     DAZKLA010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.045"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..359
                     /locus_tag="ACG85N_001373"
     CDS             63..359
                     /locus_tag="ACG85N_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFQ0224151.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(1003..1956)
                     /locus_tag="ACG85N_001374"
     CDS             complement(1003..1956)
                     /locus_tag="ACG85N_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0224152.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1979..2734)
                     /locus_tag="ACG85N_001375"
     CDS             complement(1979..2734)
                     /locus_tag="ACG85N_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224153.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2731..3690)
                     /locus_tag="ACG85N_001376"
     CDS             complement(2731..3690)
                     /locus_tag="ACG85N_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0224154.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3683..4633)
                     /locus_tag="ACG85N_001377"
     CDS             complement(3683..4633)
                     /locus_tag="ACG85N_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0224155.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4783..5235
                     /locus_tag="ACG85N_001378"
     CDS             4783..5235
                     /locus_tag="ACG85N_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFQ0224156.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5324..7591)
                     /gene="gshAB"
                     /locus_tag="ACG85N_001379"
     CDS             complement(5324..7591)
                     /gene="gshAB"
                     /locus_tag="ACG85N_001379"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFQ0224157.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7778..8776)
                     /gene="ftsY"
                     /locus_tag="ACG85N_001380"
     CDS             complement(7778..8776)
                     /gene="ftsY"
                     /locus_tag="ACG85N_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFQ0224158.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8787..9596)
                     /locus_tag="ACG85N_001381"
     CDS             complement(8787..9596)
                     /locus_tag="ACG85N_001381"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0224159.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9605..13186)
                     /gene="smc"
                     /locus_tag="ACG85N_001382"
     CDS             complement(9605..13186)
                     /gene="smc"
                     /locus_tag="ACG85N_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFQ0224160.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(13206..13892)
                     /gene="rnc"
                     /locus_tag="ACG85N_001383"
     CDS             complement(13206..13892)
                     /gene="rnc"
                     /locus_tag="ACG85N_001383"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFQ0224161.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14240..16021)
                     /locus_tag="ACG85N_001384"
     CDS             complement(14240..16021)
                     /locus_tag="ACG85N_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0224162.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(16047..16961)
                     /locus_tag="ACG85N_001385"
     CDS             complement(16047..16961)
                     /locus_tag="ACG85N_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0224163.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16988..17950)
                     /gene="opp4B"
                     /locus_tag="ACG85N_001386"
     CDS             complement(16988..17950)
                     /gene="opp4B"
                     /locus_tag="ACG85N_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0224164.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17950..18894)
                     /locus_tag="ACG85N_001387"
     CDS             complement(17950..18894)
                     /locus_tag="ACG85N_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224165.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18895..19899)
                     /locus_tag="ACG85N_001388"
     CDS             complement(18895..19899)
                     /locus_tag="ACG85N_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224166.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5563 a         4285 c         3551 g         6589 t
ORIGIN      
        1 ttattaacaa ctcttcggta gagtaaactt atcaaataaa agacttcttt ggaggaatca
       61 ctatgaagga atatataaaa aacatctatt ttatcgaaga aacacaaaat atcgagggtt
      121 cttatattga agtaaaaaca ctctttgtca atgaagataa aaccaaagca ttggatattt
      181 ataaaaaatt agcttcaaaa aaaacgaata gttttggatt gatattaagc gagtataaaa
      241 tcaaagcaga agagagttat ttctatcaat tactaaaacg gtggagtaag cttcccgccg
      301 atttttatcg aaaaatgcag atcatcaact atcaaccgct tgctgagaca catgcctaaa
      361 tagaggttgt gaaatcagtg ctttgagcta ataccgaagg tcagctgatt gaacattgtt
      421 tagaagagat tgtgacaact gcgatttgct ttgagtaata agaacgctgg acagaaaata
      481 gcttctcata ttttctagtc tagtgggctt attgccgaga agcagcagat ggaacatcgt
      541 ttagaagaga ttgtgacaac tgcgatttgt tttgagtaat aagaacgctg gacagaaaat
      601 agcttctcat attttctagt ctagtggact tattgccgag aagcagcaga tggaacatcg
      661 tttaaagaag agatcggtac aaaataaaaa tatctaaatt taagttttat ctaagtttcg
      721 ctttgtacct ttgattcaaa tcaaaggagg cgatcttgat gctgaaaaaa tggatcacac
      781 tattttttgc cactccccat tctagaatca acccgtatga tgtagataat gaattcacgt
      841 tgaaaaaaga tttctcaaat aaataaaaac catccgtaaa atcatcaata cgattttacg
      901 gatggttttt gataacttct ttgaggatag gctcttgcta tttaagaatg taagaaacat
      961 tattgttaca tagtatgaga gtttaatcct ctgttattta tgctatttaa atgcttgatt
     1021 cacatcttcg atcatcagtt tcattgattc taaaccgccc ccgcttaaat accagacatc
     1081 tggttcaagg gagatgatct gctgattttt accagcattc gtttgtgcta ccagttcatt
     1141 ggcagaaata tcatctttgc tgtcgtctcc accaatagct tttgttctgt ctactacaaa
     1201 taagatatcc ggattttttt ccagcacata ttcataagaa acactttgac cgtgagtgga
     1261 tgcttcgatt tgatcatctg cttgttcaaa gccaaataaa tcatggatga aaccgaatct
     1321 agaaccagat ccgtaggctg ataattgccc ttcattcaca agtgtgacta gtgctttttt
     1381 gtcagtagct tctgctttct ttttcgtctg atcgattgca ctagataatt cctctaattg
     1441 agttttagct tcttcttgct taccaaagat ctcccctaaa gcagtgacat tttgctggaa
     1501 agattcccat ggatttttcg catctaaaga aaggaaaatg gtcggagcaa tttgacttag
     1561 atctttttga aaatctcttt gtcggccaga aatgatgatt agatctggct gaagctgatt
     1621 aattttttct aagtctggtt cttttattcc acctgctgat tctacttttt tataggatga
     1681 aagatactca ggaaggctgc ttgtagctgc tccaactacc ttatctccaa caccaagagc
     1741 atccatcgta tctaaagaac cattgtcgaa tacgacgact ttttctggat tatttggcac
     1801 ctcgatttgg tctccattac tatctgttat cgtcaatgta gtttcagttt gtgtgctgct
     1861 tttttttgtg tctgcctgac tggtgttgtt gctatttcca catgctgcta aaccaaatga
     1921 tgctagaatc aagataccta ctactaattt tttcatctct tctctcctaa tgttagtttt
     1981 agaaataaag acagaatctg cggccttcaa tctcacaaat ccgtatgtcc atttcgtata
     2041 attcatccaa tacatctttt tttatcattt cttgggtcgt tccttgcgta aataactgtc
     2101 catctttcat tgcaacaatt tcgtctgcat aacttgcagc aaagttgata tcatgaagaa
     2161 caataacaat tgttttcccg atttcttgta ctaaattttt taaaatctgc atgatttgaa
     2221 ccgcaaaatt catatctaga ttgttcaaag gttcatccag taaaatataa tccgtgtctt
     2281 gcgctagcac cattgcaata tacgcacgtt ggagttgacc gcctgaaaga gtgtcaatcg
     2341 attgatccgc tatttcttct aacccaatgt gttccagtga ttcctgaacg atttcctcgt
     2401 cttttgtcgt caattttcct ttcgtataag gaaatcgtcc aaattctact aattctttca
     2461 cggtgatttg gacatttatt tcattcgatt gtcgtaggat cgataatttt ttggctaatt
     2521 cattggattt ccaagccttt acttcgttat ggtctaaata gatttcacct gtatcttttg
     2581 gtatcaaacg actgatcatc gacaacaccg ttgactttcc agcaccattt ggaccgataa
     2641 aagcagtcaa cttcttttca ggaatcgtta actgtacatc tttaaccact gattttccat
     2701 ggtacagttt attgaccttt tgtaaatcaa tcataggata ctcttccttt cgcgaatcaa
     2761 ttttgtgaca aaataaattc ctccgacaaa ttcaacgacg acactcactg ttgtattcca
     2821 gccgaaaatc tgctctactg cgagctgtcc tcctgtcaga aacagcatag ctaccagaaa
     2881 gcttccgata aatagctgac catgacgata agtagaaaag acacgataac tgatatttgc
     2941 gacaataaac cctaaaaaag taactggacc cacaagagcc gttgatgtcc ctgtcaaaag
     3001 actgacacaa cataaaatca aaaattcaaa acgatctgtg tctatcccga ggtttactgc
     3061 ttgttcattt ccgagatgga aaacgtctag cacatgtcga tatcgaaata acaggtacca
     3121 gcagccgata atgatcaaag aagcaggcaa aagatactga ctctggatat tgccaaagct
     3181 tgcaaataat ctgccttgaa gcaaatcata ttcattcgga tccatgatga cttgtaaaaa
     3241 ggtactgaca ctaccaaaaa gcgtacctaa aatcatccct gacatcagta ataaaaaaag
     3301 attcgctctc atttttttca aaagaaagaa aaataacaat agactcagcc cgaccatcag
     3361 cccaacgttc aataagaaaa aaactgtacc tgtctgctga agcatcgtca ctcctccgat
     3421 aaagaagaaa aggaccgttt ggacaaggac atataacgac tcaagtccca atactgaagg
     3481 agtcaaaaac tggttatgcg taattgtctg aaagctgatt gtggcactgc tgacagctag
     3541 cgagacaagg ataaatgcaa aaattttctt actcctcaat gcacatgcaa aatgccagtt
     3601 tccatatgta tggatcgtca agtacattcc tgtaacaaat aaaacaagaa caagcatacc
     3661 aataaaaata gatggctttt ttttaagcat atcgtcgtcc ccttatcaga agataaagga
     3721 agatagcccc accgatgatt ccaacgatca cactgactgg tatttcataa ggaaaaatca
     3781 acacgcgagc gacaagatcg caaaatacta agaacaaact acctgctaaa gcagttacgc
     3841 caattgtttt gtgcacttga tctccatata ttctagagac aagattcgga atgatcactc
     3901 cgataaaagg aatgcttcct accatgatca acatgactgc actagccaat gatactaagc
     3961 caatgcctaa aaactgcatc ttctggtaat ctattcccag atttactgca aacgattcgc
     4021 ccagaccaat gatcgtaaat cggtaagcaa atagccaaac caagagcaac aatggaacaa
     4081 cagcatacaa taactcataa tttcctcgta tcacagtcgc aaaattccct tgaagccatg
     4141 aagtcatatt ttgtaccaac tggaattgat aagcaaaaaa agtagcaact gaaccaatga
     4201 tattccctaa catgactcca atcaacggaa gcatcaattg atttttctgg aggaacaatc
     4261 gcgtaaggga taaaaaaagc aatgttcctg caaaagcaaa gcaaaatgct gtcaaggatc
     4321 ggacaatcaa ggatgcacca ggtaggaaaa tcatagccac taaaatacct aaacgcgcac
     4381 tatccatcgt gcccgcagtc gttggtgaaa caaatttatt ttgggtcaaa tgttgcatga
     4441 tcaacccaca aatactactg gtcgctccca caaggatcaa acaaatcgtt cgtggcaaac
     4501 gagtcactag caagatttgt aagctttgcc catctaactg gagcagttct gacaattgta
     4561 cattcgatac tccgatgaac aatgaaatac tacttaaaat aactaataat gctactagtc
     4621 gtccgtagcg catccaatcc acctttctaa ttgagaatga ttttcattct tattttaagt
     4681 gaatcgttga acaaaagcaa ccgctttttc atatgtttta ggtattattg ggaatatgct
     4741 ataatcgtaa gaaagacaaa gagaagaagg agggtatttt ttatggcaca agatgatgta
     4801 aaaagccatt tggataaggg actttatggt acacctttag tgaaccctga agaacagcat
     4861 aaatatttag ggacattcag ggaacgctgc tatcttagta tgactgttgc tgaaatgatc
     4921 caaccaaaaa acaaagaaaa ctttttaaaa gaactcaaaa aacatccaga tgcttccata
     4981 ctcttgaatg gggctctacc cctttctgtc caaaaccaat atatccagct agcaacgaaa
     5041 aacaatagcc gtttcactgt agtcaacgat tacgtcactg caaatcctga ggaattcgga
     5101 ttgctcttaa cagcaaaaga agcagtaaat gaacctgtga ttgatatcga aaaaaaatac
     5161 ccagaagaaa actcttcgcc agctgaaaaa gaaaaaccaa aaaagaaaaa aagcttctgg
     5221 gatcgtttat tttaaaacga aaaaacacat aaatttttac atgcaaaaaa agcttttgat
     5281 cagattgaca tcaaatagga caatctgtca aaagcttttt cttttaaaat acttcaggat
     5341 ataacaattt caatacattc atcgtcaatc ttctgccttt tcctgcaaaa ggataaacat
     5401 gcatatgcat ggctggattg aaatttgctt cgatgatccc atatgctttt ttatcagttg
     5461 taggatcgat ttctttatct ggtataatca aatcgatacc actaatctta gctcctagtg
     5521 cttctaccgc agaaacggcg atttttttgt aagcttcaga aaattcatca gtcatatcga
     5581 tcgaatcacc acctgtactg atatttgagt tctcccttag ataaacaatt tgattggctt
     5641 ggggaactga ttcgatcgtc aacccttgtt ctttcaacat caattgttct aattcaccta
     5701 attggatcaa ttctaaaggt gcacgatgat tcgttccacg caaagggtca ctattttttt
     5761 cttcaacaag ttctttcact gttcggatac tgtcaccaat aacattagca gggactcgca
     5821 acatgatggc ttggacttct ccgtcgatca caaagaaacg atattccgtt cccggcataa
     5881 attcttctac taaaaccgaa ctgtcttcac gaaaagcaat ggctaaacct gcttgataat
     5941 cttccagact agctccttcc ttgaaaatcg tgatacctaa cccgtaattg gttgatttag
     6001 gtttgatcac aaatgcctgc tcggcaaatc tcggataagc ttttaccgct tcttccatgg
     6061 atgaaaattc tgcgcctcct ggtacccgga acccagcttc ttttaagact tttttcgtta
     6121 ctgttttgtt ttccatgata agtgggacga tatagctgtc cttacttgtc atgtttgcat
     6181 ttttcacata ttctacatgg tcttggaatt gcagacgcaa aaattgatcc gactcatcta
     6241 atactttgac ttggacacct ttttggatgg catcgaacat aaatatctga gtagaaagtt
     6301 ccatttcgcg aaaaccggcc aactgatagg gacgttcatg gctttcttta taatattgat
     6361 gtcccaatgc cacagcaact tcttgctgac tcgatagctg tgcttcttga tagagtttcg
     6421 ctgctaacgt ttgttcagga tgatccattt gagtcaatgc ttcctctact aaataaagag
     6481 aatccaacca atccaattca accagcattt gcttcattcc ttcaagaatt tctctacctt
     6541 cttgatgaaa ttctgtttga tctgatggtt gttctagtgc tactttgtta tttcgagtcg
     6601 taccttctgc tacccattgg tcacagtctt ctttttcttc tgtccataac atgtacatca
     6661 ggaataattg taagaaccga acttgctctg gattgatacc caatcttgca taaggattca
     6721 aatcgatatt ccgcaattca atataacgaa cacctgttgt tgctaaatct gcgactttct
     6781 ttccgcctcg gaaacgaagc ggtgtataaa attctttctc ctccgaaagt ttcccttctt
     6841 cgatcattcg ctcgatatct gctaaatact gtttcataga agcataggaa actttcacat
     6901 ttggatgatt cgtatagccc agcgcactgt tgcgaatact gcgaacaggt tcttgcggat
     6961 gtgattcatc taaaaagtag ttcttctcac tcattggtga agcaccgaat aaataagtga
     7021 tcatccagcg atagcgcata aagtttctcg ctgttttcaa ataaagttct gttttgaatt
     7081 cggaaaaatc ttgaaattct tcctgatggc tgaacaatgt tcttagcaac tcatcgccaa
     7141 attcaaaatt gaaatgaata ccgcttacca tctgttttcg cttcccgtat tcttttgcta
     7201 aataacgtcg atacaagaca tcttcgaaat tttttaattt tgcaataatg atctcttcgt
     7261 ctttttccgg taaggcaggt ggcatactta atggccagat catctcttct ttcggtatag
     7321 aacgagcagt cacatcataa acagcagcga gatactgaaa taattcggga atagaatcgg
     7381 taacaggggt gatcatctct gtttgtgttt cgctaaaatc tgtttggata taggggtgat
     7441 aggatcgatc gccaaagatt gctggatgat cggtttttgc tagatttcct gcaagatcaa
     7501 cccgctgtcc ttcccgttct ataccaaatc gagcttggtc gataaaaggg cgcgcattga
     7561 catgcaataa taattgctta aaattcatca ttaagtgacc tccaattcat atgaatctaa
     7621 ttataatcaa aaagtctaag aaaaagaacg aaattgtttg agaaccgctt acaaataagt
     7681 ataaaaatga aggcatcaaa aacacagcta tgttaaaaac aatcattcaa acaaggagct
     7741 gcaacttttg tcacagctcc tgctttttag acatgggtta ttcttctttt agtaatcctt
     7801 tgaacaatcc aacgacaaaa tcatttggat caaacggctc caagtcatcg attccttcac
     7861 caagaccgac caatttcact ggtaaatgca attcattacg gatagcaagt acgataccgc
     7921 cctttgctgt accatctaat tttgtcaaaa caagaccggt cacatctgtt gtttctttga
     7981 attgttttgc ttgaaccatc gcgttttgac cagtggttgc atctaataca agaagtgttt
     8041 cttggggtgc ttctggtaat tcccgctgga ttactcgttt tattttttct aattcgttca
     8101 tcaagttcac tttattttgc agtcggcctg cagtatcaac tagtaaaaca tctgcctgct
     8161 ctgtctttgc tctttccaaa gcatcgaata cgacagctgc tggatcgcct cctgcattgc
     8221 cacgaacaac ttcaacacca gcgcgttcgc cccagacaac taactggtcg attgctccag
     8281 cgcggaaggt atctgctgct gccatcaata cttttttacc ttcgtttttg aactgatggg
     8341 caagtttacc aatacttgtt gttttcccta caccattaac accgacaaac aaaataactg
     8401 ttagtccgtc ttcttggata tttagttcat ttacttcatt gattccttcc gcttcataga
     8461 gatccacaag tttctcgatg atcgcatttt ggacttctgc cgattttttc gcatttctga
     8521 gtttcacttc ttgacgtaat gcttcggtga ttcgtatcgc tgtttcaaaa cctacatctg
     8581 caccgataag tgtttcttct aattcttcaa aaaagtcttc atcgactgtt cgaaaattgg
     8641 caaacaactc attcatccgt tcaccaaatg ttttacgcgt cttttctaaa cccttgtcgt
     8701 acttctcctg tacttcaact tcttctgttt tctcttcgcc agtaaaagca cgcttgattt
     8761 tatcaaaaaa tcccatttga ctccccctac tctaatacaa atttttggat cactttggct
     8821 acgccatttt cttcattgct ggctgtcacg aaatctgctg cttctttgac gaaaggcaca
     8881 gcattctcca tcgctacgcc tagtccagca tattggatca tcggtaaatc attttcctca
     8941 tcaccgatcg ccatgatttc ttcttgtttc aaaccaagat cttttgccaa taaagaaata
     9001 ccataagctt ttgtaacacc ttttggcata aattccaaaa gcatacttct tgttttgatg
     9061 atctcataca tttcatggaa ttctgctggt atttctttga ttttaggatc taattcttct
     9121 tggtcataag cgattactgc cttattaagc acaaattcat ctgtaatatc gcttatatcc
     9181 gctggttgga attgcagcag cttattcaac acattgtaca aggattgctt ccttggggca
     9241 ctaggcagct gtaaaaccac attgtcagac aaaacatcta gtggcagatc caattgctga
     9301 gccagtttgt ataagcgttg gatatctgat acttccatca ctttcttttc catgatttca
     9361 ccagtatcat ttttttgtac caaaccgccg ttgaaagtga tgctgtagtc accttcatct
     9421 accagtccta attcttgcag ataatgcgcc atcgcagcta aggggcgtcc tgtacataga
     9481 acgatcttga ccccttgctg ttttgcttgt gctaatgctt gtttattctc atctgaaatc
     9541 agcttttgtt catttaataa tgtaccgtcc aaatcgattg ctactaattt gatcattttc
     9601 gttcctacac tttcgtttcg ttgattttcg cctcttcatt gatatcttcc atccgtacag
     9661 aaacgatttt tgatacaccg gattcctgca tcgtcactcc gtacagtaca tctgctgcga
     9721 ccatcgttcc tttgcggtgt gtgactacga taaattgtgt gtcatcttgg aaatcgctca
     9781 agtaacgacc aaaacgtttc acgttggctt catctaacgc tgcttctacc tcatctaaga
     9841 tacaaaacgg aacaggacag acacggataa tggaaaagag caacgcgatg gctgtaagtg
     9901 ctcgttctcc tccagaaagc aaacttagac tttgcagttt tttgccagga ggttgtactt
     9961 caatctcaat acctgttttc agcaaatcag ttggttccgt cagtactaat tcagctttac
    10021 cgccgccaaa catatttgga aagacaattt tgaattcttg acggatagct tcaaatactt
    10081 ctttgaatct tgtccgcact tcatcatcca tctcatccat cgtttcaaac aattggtttt
    10141 tcgctgctaa cagatcatct cgctgtgttg ctaagaatgt atgtctttca ctgacttgtt
    10201 cgtattgttc gattgaattt aagtttactg gtcccagttt ttcaatctgt tcttttaagg
    10261 atgaaacctt tgttctactg tcttcgatat cggttgtttc ttgatagtct tgggaagctt
    10321 tctcaaatgt cagctgatat tctgactgca gataggtcaa atgattatct aaaatcattt
    10381 cagctcggtc tttttggact tctaatttgg attgttccga cagtcgtgct ttttgctcac
    10441 gattacgctc tgccaagact gcttcaagct tatcgatctc cttttgcctt ctatctcgct
    10501 gctctttggc ttttgccaac tcggctttta atgtttctcg ctgagcagat aattcgttaa
    10561 tttgtttttc caaactttct tctgtgactt catgatcaga aaagtctgct gtcaatgtcg
    10621 ccaactgctt ttctatcgct tcttgacgtt cggttgcctc tgctttttgg actcgggcac
    10681 cgcggagctg gatctgcaga tgattaaact gctctttcaa gacagcaaga tctgcttgtt
    10741 cttgtgcttt ttgactttgt acttgtgctc ttctggcttc catctggtcg ctttcttgac
    10801 ttaacgattt gatttcttcg tcgatttttt gtcgttgcga ttcaagatct gtttgtttgt
    10861 ctttcaattc gttcgtttgc ttttcgtaat cttcgataaa ctgctgcagt tcacgtgtct
    10921 caaaagttga gatttgcttt tctttttcaa aacgttcaag ttcatttatg atgttttgta
    10981 gctgatttgt cgcttcttgt tcttcaaaac gtaattgttc gcctcgtgta cgcaaaactt
    11041 cttgttcttc ggctaaacga gctgtttctt gttgcagctc ctgaactttt ttttcttgtg
    11101 cttggagttg tttatctgct tcttcaaatt cactagtcaa ctgtttcaat tcttgatttt
    11161 gtacaaacaa actgccttgg tttcctcgtt tattcgctcc acctgtcatg gatcctccgg
    11221 cattcatcac atcaccttct aaggaaacca ctcgatattg atagcggatc gtttgcgcga
    11281 ttgcattggc actagttaag tctttggcta aaaggatcgt tcctagcagg ttttgaacca
    11341 cagtttggat ttgatcggga taactgactt gttcactggc aatccctata aacccttcta
    11401 cagctgctgc ttgtgtcaat atatgagccg gtaattgtct tggtttgatt gtcgtcagtg
    11461 gaaggaatgt tgctcttcct cctctttgct gtttcaaata cgtgatggct tgccgagcat
    11521 ctttttcatt ttcaacgatg acatgctggg ctgccccacc taaagctgct tcgatagcta
    11581 acgtgaaatc tgcaggaaca tcgatcagtt cagccactgc tccgacaata cccgaaagct
    11641 gctgtttatg ctgcaagacc aatcgaaccc cttggtaaaa accaaaatag ttttcttgga
    11701 tttcttgaag gcttttctta cgagcacgta gctgctggac ttgattcatt aattgataca
    11761 ttttaggctg ttcttttgct aatgcttctt ggaacttttt cccgttttgt tggatcattt
    11821 ctaacttctc ttttgtttca gttaaagatg attttagctg cgcctgcttt tgtgtcaatt
    11881 cttctttttg taagatcaat cggtctactg atgcttctac ttccgattgc ttggctaacg
    11941 tctgcttgct ttttgctgtt tcttgtatat attgacgttc tagatacttc agttcgtttc
    12001 ctacagctgc ttgttcttgc atcaaatcaa catattgatc tcttaattct gccaataatt
    12061 ctttcgttga tttttggtat ttttctaatt cttgctgtgt ttttatgaga tttgcttcag
    12121 ctttttggat ctctgtttct ttttctgctg ctgctttcat caagctttct tgaagctttt
    12181 caaaatgttt aacctttttt tgtgcttctg ctaatgaagt ttgatattct tgcgagcttt
    12241 tttgtgtatg ctttgtccgc tcctgcaaaa catctttttg tccttctgtc tgcttcagtt
    12301 tttccgaaag atctaataat acttgctgat ttttttcgat cagacgatcg gcttgcgcat
    12361 tttctttacg ctgcttcgcc aaaatggatt cttgttcttg gatcgactca gatagtttgc
    12421 ctaattctag attgaatttc gcaagctgtg cttgtttgtt gtcccaatct ttttttgctg
    12481 tcttgatttc agcaaccatc aaagagacat cggtttgagt cagtgtttct tttagccgta
    12541 aaaactcctt tgctgcctca ctctgggcgg ccaaaggagt taactgttct tccaattcgt
    12601 ggatgatgtc ttggacgcgg ctgaggttgt cttctgtttc aaaaagcttc tgttctgctt
    12661 tctttttccg ctgcttgtat ttcaagacac cagctgcttc ttcaaagatc ccacgacggt
    12721 cttccggttt actactgaaa atcgcttcta cttttccttg agaaatgatt gagaacgatt
    12781 cttttcctaa tccagaatct aagaacaatt cttggatatc ttttaatctg cagctttgtt
    12841 tattgatgaa aaactcgctt tccccagtcc gtcgataacg gcgggttaca ctgatctcat
    12901 tgtactccaa tggcagataa tgatcactat tgtctaaaat cactgtgact tctgcaatat
    12961 tcagctgctt acgggtatcg gaaccggcaa agatgatatc cggcattttt ccgcctcgca
    13021 gacttttagc agattgttct cctaataccc agcggatagc ctctgtaata ttgctttttc
    13081 cgctgccatt tggtccaacc actgctgtga cgctattttc aaaatcaatg actgtcttgt
    13141 ctgcaaatga tttaaaaccg gctatttcaa ttcgtttcaa atacaaagct ttccctcact
    13201 tacttttaca gtgctgccaa tgcattttcc gccgcagctt gttctgctaa ttttttcgat
    13261 ttcccttgtc cagtgccaat caattgatca tctacataaa cttcgatcca aaatactcgt
    13321 tcatgagctg gaccttcttc attgatcaat cggtattcga ttgatacatc acctgatttt
    13381 tgcaagactt cttgtagttt tgttttatga tccatctcat gtgaaaaagc acctgctttg
    13441 actttaggaa aaatcacttt ttcgatgaat gcatgggtcg tgtcgaatcc ctggtcaaga
    13501 tataacgcgc ctaaaaaagc ttcgaatagg tcgcaaagca atgctggtcg cgtacgtcct
    13561 cctgaattct cttctccctt gcccaacaaa acatattgat caaagtgaca ttctttggca
    13621 aatttcgaca agctgtcttc gcgaacgatc gcagctcgtg tttttgtcaa ttttccttct
    13681 ggcaaatgag gatataaacg gaataaatac tgagaaacca tcagttctaa aacggcatct
    13741 cctaaaaatt caagtcgctc attatccgat aattgcagat tgcgatgctc attcacatag
    13801 gatgaatggg taaaagcttg ttcaagcaaa tttagatcgt gaaaaacgat gttatagttt
    13861 tcttttagtt cttttgtcag ctggttgtcc attttccaca ttccttttat ggatattttt
    13921 tcgatacttc cttattatac tggttttttg tcaaatttga aatggttcgg tcaaaaggta
    13981 agtaaagctt taataatcct agtgattgac gagaagttgt gaaaaattcg tttagtttcg
    14041 agtatgaaga aaaaattttg tgaaaatggc tttccatatt tttgcataaa tttgacttaa
    14101 taccgagaaa ctggactttg aacaccgttt attggagaag tcatgaaatc agcgccttga
    14161 cctgaatatt agagaaataa acagaaaaat atcaaaaaac cgaaaaagga catcgccctt
    14221 tttcggtcat ttttagtgat tatgaacgtg aatctgctgt aacagaaaca tttgcccatg
    14281 ggtttggtac atcataagac caatcccagt ctttgacacg gtcgttgact ggcaatactt
    14341 cattacggaa caacgttgga atcgcaaatg cttcttcaaa tgcatattgt tgccattttt
    14401 gatatgcttc tttttggtac tcttcatcaa atgctttgct tgaactgata tcgttgatca
    14461 attttgtatt ttcttctgat tcataacgag tgtagttgaa cgcagcattt ggtccccata
    14521 gtccacttgg atttggatca gttcctgtat tccaagcagc ttggtaaaca tcaatttcag
    14581 gatcatcgtt ttctaattta tcatagaaag aattgaattc gatcagacga cctgttgtgt
    14641 attggacatc caaaccaatt tctttccatt gttggatata gtaatctgcc aatggttgcg
    14701 ctgtttcccc accggacatc gaagcaaagt tgattgttag tttatttcca tctttgtctt
    14761 ctcttaaacc atcgtcatcc acatctttgt agccagcatc atctaatagt tttttcgctt
    14821 tgtctagatc ttgtgtgaat cctttcatct catcatcatg gtatgactgg aatactggag
    14881 gaatcaatga agtcgcattg cttctcaaac ctgcatagaa acgttcacct actgcattat
    14941 tatctaatgc atagcccatt gcttgacgta atgatttatc agccattttt gaatctggat
    15001 tgtattccac actctttgta tctgcattcc atttacctaa cttgaatcct agataagtat
    15061 aagaagtttg ttcacggcct aagtttgtat agccagcaat ttctttatag gtatcgtaag
    15121 tatctgttgg catttggaaa atcatatcat ataatttaga gttcaatgct tcagttgcac
    15181 ttgccatcgg tacagatttc atcgtgatct ttttcaattt aggcgtacca ttccagtagt
    15241 gctcgtttgg caagtattct actgattctc ctgcaacgat attactcatg taataagggc
    15301 caaaagtcac tggattttta cgtacttgat cgcttgattc catatctttg attgctacac
    15361 cttctaacgt atgttttggt gctgcatatg accagatacc accacccgat tgaagcatgc
    15421 ttgggtttac ttctttatat tcgatttcaa ctgttttgtc gtctattttt ttgattccgg
    15481 agattgtgtc ggcacttcca tcatgatatt cttccatacc tacgatgtta cggaaagtgt
    15541 cgtcataacg gataccagtg taatctttat tcccaatgat ttcgtaaggg aaaatcacat
    15601 cgtccgccgt tacatcttca ccatctgacc atttgacgtt gtcttttact ttgattgtcg
    15661 cttttttcgt atcttcatct agatcaagcg ttgcagctcc gtcgtctgtg attctgaaat
    15721 cgtcatcgtt tgcaaatagc ggttcatgag aaggagccat aaaagctgca tcatatgaat
    15781 cttggtaaaa ctcccactgg aacaatcctt taaactgact gtccgttgcg accgcagctt
    15841 ctaattcgcc gtcttcgatt gcatcatcat tattttttgt tgcaattgga aaagttaatt
    15901 cctctttgtt attgccgttg cctccagagt tgtttccaga ggtatctgta cctccgcccc
    15961 cgcatgctgc aagaacgaca gcagacatca gcgctgcaaa tccaaaaatc gttttcttca
    16021 ttcctatttc ccccttcgct tctatactag cctaaccgtt ggcgcgcatc tgcagaacgc
    16081 ttgaatgctt gtccaacata attgatactc aacatcaaaa ctaaaataag gattgatgcg
    16141 ggtaaccaga tccattgccg attcactaag acttctccgt tacttgcata accgatcaat
    16201 gtacctaaac taggtacatt tgccggcaag ccgaaaccta agaaagtcaa agttgtctcg
    16261 ataccgatgt ttgccgcaaa gttcatcgtc aaattagtaa tgattaatga acttagattc
    16321 ggcatgattt cgcggaacat gattttaaaa tcattggttc ccattgtttt tgaggcactg
    16381 acataatctc ttcttgcttc agataatgtc ttgcttcgga ataatcgggc tttagctacc
    16441 caataaaatg cactcatgat gaatataaag gaccagacat tgtatctgga aataatcgat
    16501 acgaagacga taatgatcag catgatcggt agaatcatga taaaatcaac gatccgcatc
    16561 aagatgttgt caatgatccc tccgtagtag ccagagatga tccctaatcc aaccccgatg
    16621 atcgaagtaa ggatcgtgat cgcaaatccg atcgtaacag agtttctagc acctatgatc
    16681 aattgtccta atacatcacg tccgccttca tctgctccca gaatgaactt ttgtccttgt
    16741 gtcgaaactg ttcctggtgc agcgtatttg tcgaagatac taacatacat cactttgtct
    16801 tgatcggtca gcatcgcacc gataaagatc actaataata aaaccactag caaacctaat
    16861 gaaaaaatag caattttatc ttttttaaat tctcgagcaa tcattcgtat ccccatagga
    16921 ggaatacttt cttgaactgc tgtattgttt ttttcactca tcgatccatt ctcctttctg
    16981 attggtttta ttgaatccga atacgcgggt caacgatact catgatgata tctgaaagta
    17041 atgtccccac tagcgtcgcg attcctaaaa tcaatactaa ggatgtgatc acggtatagt
    17101 cccgttggcc gatggaatca ataaatagtt ttccgactcc cggataacca aaaatctttt
    17161 cgataactac agaaccagca atcaacgaag tgatttcata ccccatttga gaagccatcg
    17221 gcaacgctgc atttcggaaa atatgtcgac tgaatacttt attcgtcggt acccctttgg
    17281 aacgggctgt tctgacaaaa tcgagcgatt tcgaatcgat tacttcactt cgcagatatt
    17341 ggattgtgac ggcagtcgcc aaaaatgctt gggtaattga tggtaacaga atatatttca
    17401 aacggtctat ccattgagtg ataaatccac cttgcaaacc tagtgtttgt gatccacttg
    17461 ttgggaacca gcctaaacga taaccaaaaa caaatagcat gatcaagccg aagataaata
    17521 aaggaacagc aaaactaaag aagttgtaga ttacgacaat cttatccaag attgaatttt
    17581 gataccggcc agcaagcatc cccagaggca cagcaatcaa ataagtaagg acaactgtca
    17641 agatcgatag atataaagtg ttgatgatcc gttcgccgat caagtcagca acagagcgtt
    17701 tgtataagaa actttgacca aaatcgcctt gcaaagcatt tccgatccaa cggaagtact
    17761 ggacatacca tgggtcattc aagccagctg cttgtcgcat ctgttcgatc actgccggat
    17821 cctgatttgg attgatcaaa ccggtaaatg gatcccccgg catcgcttgg gctaataaga
    17881 agatcagaac acttaaaata ataacttgcg ggatcatcaa taaaacacgt cgcagaatcg
    17941 ttttccacat tagcttgcac ctccatcttt caacgccact ttgtgtgtag ttgtgatcgt
    18001 acgcaaatcg tagactcgac cagtcgcatc atagtattct ttttggtttt ggatatactc
    18061 ttgttctact cttcgtcgat tttctttgtg gctttcccta ttttctacat cgatttgcgg
    18121 aatagctgaa agcagacgtt tcgtatagat atgtcgcggg tcattataaa tgtcttctct
    18181 tgtaccgatt tctacaaatc gacctttgta cataattgcg atattgtcgc acatgtgttt
    18241 tactacacct aaatcatgtg aaatgaataa atagctgagg ccgtattctt gctggattcg
    18301 tttcatgaaa ttcaatactt gcgcttgaac agatagatcc agagcagata caggttcatc
    18361 ggctacgatc agttttggat tggtagcaac agcacgggct actcctagac gttgacgttg
    18421 tccgcctgaa aattcatgag gatatttata tagcgcatct tccggcatcc caacgatatc
    18481 tagcagtcct tttacctttt tcttttcttc ttgatcgctt aaccgttcaa aatttcggat
    18541 gggttcagca atgatatcca acactcgctt tttaggattc aaactggaca ttgaatcttg
    18601 gaagatcatc tgcacatcct tgttataacc catttttttg cggttgcttc gcttcgttac
    18661 atcttttcct tcatacatga tttgtccggc tgttaccttt tctaatccca caacggcttt
    18721 tcccgtagta gattttccag agccagactc tcccactaaa ccatatgtct ttcctttttc
    18781 gataataaaa tccacaccat ctacagcata tacatgatcc gttactcgat tgaagaatcc
    18841 actgcgaata gggtaatgaa ctttcaggtc tttgattgtg attaattctg tcatctacac
    18901 ctctcctttt tcgtctctaa agtggaaatg cttatagcag gagcaacgta ccaagtggtt
    18961 cggtgcgact tcatgaagga taggctcttc ttcatgatcg cttgctggta tccatggaat
    19021 acgtggtgca aaccgacaac ctttacgagg catgtttttc agagaaggaa cgacaccttc
    19081 gatcacatgg agttctgcat catgatttcc ttcttgtggt atagagttca gtaaagagcg
    19141 tgtataagga tgcttaggat gatcaaacag ttcttttaca cctgctactt caacgaactg
    19201 tccgccatac atcactgcca ctcgatcagc catttctgct acgactccta agtcatgagt
    19261 gatcaagatg atccctgttt ttgtttcttc ctgaaggtct ttcagtaaat caagaatctg
    19321 tgcttggatg gttacatcta gggctgtcgt cggttcatct gcaatgacga ttggaggttt
    19381 gcaggcgatt gcgattgcga tgatgacacg ttgtctcata ccgcctgata gttcatgggg
    19441 atattggcgt ccaactctcg caggattagg aatccctact tgttctagca attctagtgc
    19501 acgagcttga cgctgttctg cattcatctt tgtgtgatag gaaagtcctt ctttgatctg
    19561 gtcttcgatt ctcatgagag gatttaatgc agacaacgga tcttggaaaa tcattccgat
    19621 atcatttccg cggattttat tatagagcgt ttcgttcagg tcgattaaat tcagatcgtt
    19681 atataaaatc tcacctgtaa tttttgtgtt attaggatca agtaatccca taatcgtagt
    19741 agccaaggta cttttcccgc acccagattc cccaacgatt gctaaaatct cgtttttgtc
    19801 taaagtaaat gaaacgtcat ctactgcatc gtaatagtca tctttgagtc ggaatcctgt
    19861 atgtaggttc tgaacttcta ataattggtt atcggacaat cgaatacccc tctctgcttc
    19921 ctaaatggaa gtttttctta ataaaaaagg acattacaca gaataaatcg ttttataaca
    19981 aaggttat
//
LOCUS       DAZKLA010000046        19694 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000046 DAZKLA010000000
VERSION     DAZKLA010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.046"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..210)
                     /locus_tag="ACG85N_001389"
     CDS             complement(<1..210)
                     /locus_tag="ACG85N_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0224167.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDI"
     gene            complement(216..446)
                     /locus_tag="ACG85N_001390"
     CDS             complement(216..446)
                     /locus_tag="ACG85N_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224168.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            1063..1725
                     /locus_tag="ACG85N_001391"
     CDS             1063..1725
                     /locus_tag="ACG85N_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224169.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1804..5031
                     /gene="fss3"
                     /locus_tag="ACG85N_001392"
     CDS             1804..5031
                     /gene="fss3"
                     /locus_tag="ACG85N_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0224170.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            5145..5459
                     /locus_tag="ACG85N_001393"
     CDS             5145..5459
                     /locus_tag="ACG85N_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0224171.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5472..5846
                     /locus_tag="ACG85N_001394"
     CDS             5472..5846
                     /locus_tag="ACG85N_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0224172.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5856..6200
                     /locus_tag="ACG85N_001395"
     CDS             5856..6200
                     /locus_tag="ACG85N_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFQ0224173.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            6271..7623
                     /locus_tag="ACG85N_001396"
     CDS             6271..7623
                     /locus_tag="ACG85N_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0224174.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7683..8825
                     /locus_tag="ACG85N_001397"
     CDS             7683..8825
                     /locus_tag="ACG85N_001397"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFQ0224175.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8850..9104
                     /locus_tag="ACG85N_001398"
     CDS             8850..9104
                     /locus_tag="ACG85N_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224176.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            9360..10544
                     /gene="mobT"
                     /locus_tag="ACG85N_001399"
     CDS             9360..10544
                     /gene="mobT"
                     /locus_tag="ACG85N_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0224177.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            10541..10678
                     /locus_tag="ACG85N_001400"
     CDS             10541..10678
                     /locus_tag="ACG85N_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0224178.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            10683..10904
                     /locus_tag="ACG85N_001401"
     CDS             10683..10904
                     /locus_tag="ACG85N_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224179.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10917..11417
                     /locus_tag="ACG85N_001402"
     CDS             10917..11417
                     /locus_tag="ACG85N_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0224180.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            11514..13361
                     /locus_tag="ACG85N_001403"
     CDS             11514..13361
                     /locus_tag="ACG85N_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0224181.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            13364..14260
                     /locus_tag="ACG85N_001404"
     CDS             13364..14260
                     /locus_tag="ACG85N_001404"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0224182.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            14310..14699
                     /locus_tag="ACG85N_001405"
     CDS             14310..14699
                     /locus_tag="ACG85N_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0224183.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            14686..16185
                     /locus_tag="ACG85N_001406"
                     /pseudo
     CDS             14686..16185
                     /locus_tag="ACG85N_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            16933..18759
                     /gene="ltrA"
                     /locus_tag="ACG85N_001407"
     CDS             16933..18759
                     /gene="ltrA"
                     /locus_tag="ACG85N_001407"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0224184.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            18872..19694
                     /locus_tag="ACG85N_001408"
                     /pseudo
     CDS             18872..19694
                     /locus_tag="ACG85N_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         6654 a         3219 c         4309 g         5512 t
ORIGIN      
        1 gatatcaaac aaaaagtata catctttctt cattctagtt tttgcagcag ggattttaaa
       61 gtagttctta ttagaatagt agagattgat taataagcta tcttcgattg ctagaaagaa
      121 aacttctgaa tcccacacct tataaaaatc tttgataaat ctattcgaag gatcaaattt
      181 aaaccataat tgtgtctcat taaaaagcat agccattact ccgatctatt tttaaactta
      241 gtttctacct ctaatatata tcgagttttt attttgcctt cagataatac tgtttctttt
      301 ttcgcaatta caggttggtt attttcggaa aaagctaata tagctaagat tgaaacatcc
      361 atctggaatt tatctttttt gctgctttgc tcataaaagt ctgcatattc atcactgata
      421 ttttttctaa taaagtcttc catcataaaa atcacctcaa aacgattata cgaacttacg
      481 ttctcgttgt aaagcgatat ttgaggggca aaaagggggc aaaaatacgt ttacctttga
      541 tatatctcag attgaacata gtgtttatta tatgtcggta ttgttgattt atagctgttt
      601 tgagggtatt agatacataa tagaatatag tggttttgtt tacgtgcatt acacggaaaa
      661 gctgctcgta tcaaagaaat cagaagataa acgcggaaaa cttccttgga aatctgaaat
      721 ttgtgaaaag tccttgtata tcaagggctt tttctttttt ctctatcaca gttttaagcc
      781 atttttggct gttttggacc atttttcaat catattccct taaaaattgt cctagattgt
      841 ccgacattga ctatcagtgt cgtcaaaata gggcaaaatt tagtagtcag cttgacgaca
      901 aatataatac gaaaaatacc aaaacattta ccgaatttag caacttttga acccaagttt
      961 ctcaaagggt cagctattag caaaaaagac aaaacatgta cttttctact aaaaagttga
     1021 tgtgataggc ttctatctaa ataactatta ggagttttta tcgtgaagca aaatgatgga
     1081 cgtatcattt ggaaaaatca aagtgagtta aaactgattt taacaattaa tgaattcatc
     1141 gaaaaacacg gaattacatc tagccgtcaa taccagaaaa agctatcaga aaatccaaat
     1201 tctgcaccaa gtatgtggtt cattaacaaa aaatatggat catgggagaa tttattgatt
     1261 agtataggac gagaaaatac tgggtatgga aaatgggcta gaatgtcaga acaagaatta
     1321 ttggaaatag tagaagcctt tattaagtgc gagaagataa cctctcaacg aatgtacgaa
     1381 caaaaatcag tagggaagaa tattccttca cttagtacca ttaaaaagat gttaggagat
     1441 atacgtccac tttttaaaga aaaaaatgat ggatctcgat ttactgattt cgaactgctg
     1501 ttagaactaa aaaatgaaat aatccgatta aaactacagg atgatttatc aatgacaaag
     1561 tttcgaaaac tagttcaatc cccaaaacta ccatcagttg atacaattat gaaaagaacg
     1621 aacaaaaatt gggaggaatt gatggcagag attggatttg attatcgaag aatcaaaatt
     1681 tataagcaga gaaacaacct atccaagaca aagaaaacta aatagtatca ctctaaatca
     1741 gacgttcaaa attaaaattt ggacgtcttt tttattaccc aaaaacgaaa ggagatttat
     1801 tttatgaaaa agccaagatt taagaattgg cgtttgctcg caacactgag tctattatgc
     1861 caaacaattg gtggatcgct tggcccaacg attgcctttg ctgatgaaat tactcatcca
     1921 caaacggtaa ctgtggaatt ggatttggct caccagtatg ctgtcgaagg tacatttagc
     1981 gatggtcgtc ccatgtcaga agttaccgtt ccgcactatg cagtttacaa tggtgtgaag
     2041 caagatattt tctgtattga gccaggggtg ccgatttaca atgagttcac tcctggttat
     2101 gagaaaaatc cattgcctga tatgtcggag aaagcgaaat tagtttccgt tttatggaag
     2161 gaagctggta cggatgtgga tacacatatt gtggcccaga agatgatttg gcaagaagtt
     2221 aatggctaca cgcttcactc aattaaacgt tctgatggta gtgcagtaaa tatcgcagcc
     2281 attgagtcaa aaatcaatca agccattgcg gattatcaaa agaaaccaag cttccataac
     2341 tccacagtga aaaccgtgtt aggtcaatcg actactgtga cagatacgaa cggcttgaat
     2401 ttatcggagt ttgatgaagt tgtcgagaat atcgcaaata ttgattatcg tgtgaacggc
     2461 aatcaactag tgattactcc aaatgccaat tcaaaagaga gtggtgaatt aaccctaaag
     2521 aagtcagcta atacaggaac accggtagct tataaaatgg ctggtcaaca aactttgatg
     2581 gcgggggcaa ttgataagcc caatacctac acaatcaaaa ttgatgtgga aactgagggt
     2641 tctttgaaga ttaaaaaagt cgataaagaa acaggaaatg ttgtaccggg aaccgttttc
     2701 catttagact ttggaaaaac attacctgca aaagacgtga ccaccgataa agaaggcatt
     2761 gcgacattgg atgagattcc tcatggcgcg aaagtaacca ttactgaaaa atcagtgcca
     2821 gctccttata cgattgacac tacaccaatc accgcaacca ttaaatctgg tgagactatt
     2881 tctgtgactt caaaaaatac tcgcgaaaaa ggtcaaatca ttttgaataa aatgggggtg
     2941 gaaacaggaa ctgatctttg gaacgacaaa tactcattag ccggaaatac gtttgccatt
     3001 cgcaaagaca gtccaaccgg tgaaattgtc caagaaatga ccacggatga aaaaggtcat
     3061 gcggaaacac caaaagagat tgccaatgcg ttggaattgg gaacctatta tgtgaccgaa
     3121 accaaagcta gtaatggctt tgtgaatacc tttaaaccag taaaagtcga attgaactat
     3181 gtcaatcaaa ccgtggctct tgttaccagt aacgtaaaag ggcaaaacca agaaatcact
     3241 ggtgaaacca ctttgacaaa agaagacaaa gataccggta atgaaactca aggtaaagct
     3301 gagtttaaag gagctgaata tactctcttt actgcaaaag atggtcaagc ggtcaaatgg
     3361 agtgaggctt ttaaaccaga gttagtgaag ggaacgaaag cttctgatga aacggtgact
     3421 ttgactttag atgaaaagaa tcaagttgct gttaaacact tagcgattaa tgagtattac
     3481 tggcaagaaa ccaaagcacc tgaaggatat accttggatg aaacaaagta ccctgtttcc
     3541 atcaaaaagg tggatgataa cgaaaaaaat gccgtgatta cccgagatgt tacggcaaaa
     3601 gaacaagtca ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgctgggact
     3661 gccgaaactg gcttcaatga tctgaccttt aaagtatcgc cattggaagg aaccagtgag
     3721 attacagatg ccgaagatga agcaattaca gcttataacg agcaactagg ttttgatggc
     3781 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaggctcca
     3841 gaaggatttc aaaaaattac gccattagaa atccgttcta cttttaaaga aaataaagcg
     3901 gattacacta agagtgaata catttttacg attaccgaaa aaggacaaaa acaaccaatt
     3961 aaaacggtga ccgttcctta cgagaaactg acgaataaag cattttctgt tagcttgaat
     4021 cgtttgatgc tctatgattt acctgaagaa gaagatagct taacttctct tgggacttgg
     4081 aaagacggag gcaaagaatt gacagacctt gattctaccg agctagttga taaattgagc
     4141 tataacttgc atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggaa
     4201 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtaat tgccgaaaca
     4261 accgcaacgt tggcgaataa agagaaaaca ggaacttgga aaattttgca taaattaacc
     4321 gcagaacaag ttttggataa aaccatcgtc ttattcaact atgtgtatga aaacaagaaa
     4381 ccctatgaag caggtgatga tccagtagcg aaagatgcta gcttgaacaa tcaagcccaa
     4441 actgtcaatt gtacggtgga acgccatgtt tccatccaaa caaaagccca cctagaagat
     4501 ggttcgcaaa cctttactca tggggatgtg gtggatatgt tcgatgatgt gtcgattacc
     4561 catgatgtac tggatggctc aaaagaagct tttgaaacaa ttctgtatgc attacttccg
     4621 gataatacga ccaaagaaat ttggaaatct ggcaaaattg attatgaagt gaatgacaaa
     4681 gaattcacta aaaccgtact tgcggaaaaa gtggacacaa gtaaatatcc agaaggtaca
     4741 agttttacct tcaaagaaat taactatggc aaagacggaa acatcaatgg gaaacacaat
     4801 gaggatctaa aagaaaaaac tcaaacttta acacctaaaa aagttccgac aacaccaagt
     4861 gcaccagaac aaccggaaat accaactgtt ccaagtgact ctcaagaatc tagtcctaca
     4921 gtaaagacat tcccacaaac aggggagaaa aattctaatg ttctgctgtt tattgggttt
     4981 accttgatct ttgcgactgc tgggtattat ttctggaatc gccgaaacta aggtgatgtg
     5041 atgaaacaac taaataggaa aaaccaacgt gcgcgaccgc ctcctaaaaa aagtggtcgc
     5101 gctatttttg tatccaaaac taaaaatgaa aagaggaaaa gaagatggaa ttgaaatttg
     5161 ttgtgccaga tatggccgaa acctttggaa agataaggta tgccggcgaa ggcgaagttt
     5221 taactgaagg atatgggcgg aatactacag taattggtcg tagttatcat ttgtattcca
     5281 gtaagcaacg agccgatgat attgaagtgg tggtagctgc ggaagccggc gaaaaggatt
     5341 ttgatcaaga tcaaccacta aaagccgtga atccccattt ggttgccaaa ggctatgaga
     5401 ttgagaatcg tgggtttacc gactacgtgt tgtatgtcga tgatttagtg aaagcatagg
     5461 aggaaaagaa gatgagatta acagaaggta ttgttgtaga ttcaggacta acgtttggga
     5521 aattacgatt ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacggtta
     5581 gtaacgaggt aaaagaacgg acttataatt tgaaatcctc tgcgcaaggt cgaatgatcc
     5641 aagttagtat cccagcaaat gtacccttac gcgagtttgc ctatgacgca gaggtagagt
     5701 tggtcaatcc aatcatggat acggtcgcca attatgtttt ccgagaagga actactgtca
     5761 attggttcat taaggccgat gatttagtct tgaaacgaca accagatcag ggaaattcta
     5821 caaaccaaaa tgaaggaaag aaataggtga agcgtatgga aattaatgtt gaacatattt
     5881 tagattgtct cgatcaatat ggaaaaggtg agctaacgga ggaacaactc actaaagcat
     5941 taacctatga tgaaaaaatg tttctgatta tgcatcaagg gttactggaa gtaggtaacg
     6001 atgccgaaga agattttgat gtactgaatt ggttagggga agaagagtcc ttctttatgg
     6061 ttatcgaagt aaatgaaagt ctatgtcgag aagccgaaac cgtattggaa gaaattggtg
     6121 tggaaatgcc ggatgccatt gaagtttttt taaagcaact agtggaaacg aaacaactcc
     6181 ctatagcagt aaatgactag cataactgct tgaaattgga tcaatgaaat cctatgaagc
     6241 ttttttcgat tggaggtgga aaccataaaa atgtacaaag ggcatcgtat acgggcagga
     6301 gatcagcact tggtttatca ctttgttctt ggctggctac tcgctctgtt catcggttgg
     6361 atgagcgtct tttattttca agagcttaga caatttgata tttctaagct atcactttct
     6421 actatagaaa tcgttcgttc tataaaagat ttaatctatt tgctaggaag tctcgttctt
     6481 tcaggaagca cgatgttgct ttatatacac ttttttcagg atcattggcg cagtttgtgg
     6541 catcgacaaa agctagcccg gatgattcta gaaaatcatt ggtacgaagt aaaacaaact
     6601 cagagtgaag gatttttcaa agacctcaat agtagtcgaa ccaaagagac aatcagttac
     6661 ttccccaaaa tctattaccg tatgaaggat ggtttacttt ctattcgcgt tcaaatttca
     6721 ctgggaaaat atcaagatca gcttttaaag ctggaaaaga agttagaaag tggtctatat
     6781 tgtgaactag tagaaaagga actcaaagac tcctatgtgg agtacacttt actgtatgat
     6841 ataattgcca atcgaattgg gatagacgaa gtagtggcag aaagtggggc tttgcgattg
     6901 atgaaaaatc aagtatgggc ttatgattct ttacctcata tgttaatcgc tggcggcaca
     6961 ggtggtggga agacttactt tttactcact atcatcgagg ctttattaaa atctgatgcc
     7021 gagctttttg ttcttgatcc caaaaatgcg gacttagccg atttaggtac ggtaatgcct
     7081 catgtctatt cccaaaagga ggaaatttct gcttgtgtgg aagatttcta tgagcgcatg
     7141 atgactcgta gcaaggcaat gaaagaaatg cccaactaca aaacaggaga gaattatgcg
     7201 tttcttggac ttccaccaaa ctttttaatc tttgatgaat acgtggttta tatggaaatg
     7261 ttaacgacga aggaaagtgc ggtgattttg aataagctca aacaaatcgt gatgttaggt
     7321 cgtcaatctg gtttctttct gattctagct tgtcaaagac cggatgcgaa atatttagga
     7381 gacgggattc gcgatcaatt taatttctgg gtggctttgg gtcgtatgag tgaactggga
     7441 tattccatga tgtttggcga ggttgataaa aaattcttta tgaagcgtat caaaggtcga
     7501 ggctatgtag atacgggagg gagtgtgatt agtgagtttt atacgccact tgtaccaaaa
     7561 ggatatgatt tcttaaggga aattagcgac attggtggat taaacgttaa tacggaagga
     7621 taaaataata gtatgtagcc taggagtgtc aagaatcctg ggcttttttt acgaggtgat
     7681 ttatgacatt tttagattta ttcgctggga ttggtggctt ttgcttaggt atggaacaag
     7741 cgggtcacca gtgtatcggc ttttgtgaaa ttgatgattt tgctcgccag agttataagg
     7801 caatccatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta tcagacgagt
     7861 ttattcaatc tctcggacca gtggatatcc tttgcggtgg atttccgtgc caagcttttt
     7921 caattgcggg aaagcggcaa ggattttccg atactcgagg tactttattc tttgaaatcg
     7981 ctcggttcgc cgctattctc caacctaagt ttttattcct tgagaacgtc cggggattac
     8041 tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgctggat ggattggggt
     8101 atgatgtgga atggcaagtg cttaactcaa aggcctacgt tccccaaaac cgtgagcgga
     8161 tcttccttat cggacattct cgagacgcat gtaccgaaca agtatttcct atcattggat
     8221 cgtctccaac atctgatcaa aacatcagaa acttattaaa tatcaaccct tccaatcgag
     8281 gaatgggggg acaagtctat gggtcagatg acgtagcgcc taccttgacc ggtgatgaag
     8341 gaatcaaaat cgcattgccg gtagatgatg gaatttctgt agcgggtatg ttactgggaa
     8401 attttgaaca aggcaatcgg gtttatgaga taactggaac agcgcctact ttgtcaacga
     8461 aacaaggcgg aacgaagatc atgcttcgga aaaacgaaac agaatatcaa gaggtgaagc
     8521 cagggaacag tgtgaacctg gcttttccta attccacaag tcgtcgaggc cgtctgggaa
     8581 aacaaagtgt gcatacctta ttaacaggag accaacaagc agtcgtcacg gatcagtatc
     8641 aaattcgaaa gttaacacct agggagtgct ggcggcttca gggctttccg gactgggctt
     8701 ttgatcgagc ttctcaagtc aattcagata gtcagttgta taagcaggct ggaaattctg
     8761 taacagttcc ggtcattttt gatattgcta gaagattaaa ggaggtaaaa acagatgatt
     8821 tatgatgagt tgattgggga atctactggg tgattggcaa gattcagtca gatccggaat
     8881 tggaagaaga actgtaccgg ctcaattttg atattcgcaa aaacggggtg aaagtccctg
     8941 atgatccgta tgtaattgat gaggaaacgg atgctcgaat ggagctgaat caagtcatca
     9001 gtgaattgga acgaattgcg gactttgcta aagaacctga tatccgccaa tacttgtttg
     9061 agataaaagc agagttggaa attgagggta ttaccgcgga gtaacggcca aataaaccac
     9121 tgataggcac tgaccgcggg ggatttgcga agcagaaatc ctccgtggtg ctgtgcctgt
     9181 ttatggacgg cgttagccgg ccatgatgtc tcaccccccg tatctaacag gggggtacaa
     9241 aaacacaaaa aataagcaag caacgataag gaatggctgt cctatcaagg ataataagca
     9301 atttgtgtag atgttaactg atttgaaaaa gttaacacct taaatttgga ggtgagaact
     9361 tggcacaaag gaatttagat tatcggctat taaaagaccg gcgtaatgaa tatggcgttt
     9421 cacaaaataa attagctacg acttgtggac ttagtcgccc gtacttaaat cagatagaaa
     9481 atggtggcgt aaccgcatcg acaaaaacca tgaggaaaat ttttgaccaa ctggaaagtt
     9541 tcaatcccga gttaccttta acgttactgt ttgactatgt aaggattcgc tttcccacaa
     9601 cggatgcgag gaaaatcatt caagagattc tccatttgaa atttgattat atgctccacg
     9661 aagattacgc cttttactcc tatcaagaac aatatgttat gggagatatt gtggtgatgt
     9721 tgtcacatga agaagataaa ggcgtccttt tagaattaaa gggtcgtggt tgtcggcagt
     9781 ttgaaacttt tttactcgcc caaaagcgta gctggtatga ctttttcgaa gattgtctaa
     9841 aagccggtgg cgtgatgaaa cgcttagacc tggcaatcaa tgatcgagtt ggcctgttag
     9901 atattcctga tttaacgaaa aaatgtcaga aggaagaatg tatctccttg tttcgtacct
     9961 tcaaaagcta tcgttccggg gaactgttga aacctgatga taaagatggt atgggcaata
    10021 ctttgtatat tggcagtctt aagagtgagg tctatttctg tttgtacgaa aaagactatg
    10081 agcaatatat caagttaggg attcctttag accagaccga aacgaaaaat cgttttgaaa
    10141 tccgactgaa aaatgatcga gcttatcatg caattcaaga tttgttaaaa ggtcgcagta
    10201 ttgaaagtac cacattttcc attattaacc ggtacttgcg atttgcggat aaggtggaag
    10261 gcaaaaggcg aactaactgg cccttgaatg aacaatgggg gcgcttcatt ggacggaatc
    10321 agaaggaaat tcaattaact tctgaaccga agccttatac cattgaacga acgttgaact
    10381 ggcttggacg acaagtcgcc cctacgtgga agatggccaa ggagttggat cggttgaatc
    10441 agacaacgta cattcaggat atggtaaata atactcgact atcagaccgg cacaaaaaga
    10501 ttttggagca acaaagtatg gcaattgaaa atttgattat atgaaaagag gaatcgtgat
    10561 ggaaattgtg aaagatattt tattggtgtt tggtggcagt atgcttggtg ttactgtgat
    10621 gtgcttgatg catgcaagtg cagcggcgga ccgagcaatg gaaaagaaag gaaagtgatg
    10681 aaatgaattt cggtcaaaat ttgtatcaat ggtttttaac caatgctcaa tctttagtct
    10741 tactagcgat tgtggtcatt ggtttgtttt taggatttaa acgcgagttt tccaaactga
    10801 ttggcttctt ggtgattgct ttgattgcgg taggtctggt ctttaatgct tctggtgtga
    10861 aggatgtctt actcaatttg tttaaccgaa ttgtcggtgc ctgaggggga atcacaatgg
    10921 aacagatacg tgtttatatt gcgaaccttg gaaaatacaa tgaaggtgag ttggtggggg
    10981 catggtttac cccacccgtc gattttgatg aagtgaaaga acggattgga ttaaatgacg
    11041 aatatgaaga gtacgcaatt catgattatg aactgccttt tgagattgat gagtatacac
    11101 caattgagga aattaatcgg ttgtgtggtt tagcggaaga gcttaaagga acgccaattg
    11161 gtgaggtcgc ttcagagatt caacatgcct tcttcaattc tttcgaggaa atggtagagc
    11221 atgtggatga tatcatctat tatcccgatt gtaatgacat gagtgatgta gcgtattact
    11281 tgattgtaga agctggtgat ttaggcgaag taccagaaca cttgaagacg tatattgatt
    11341 ttgatgcgtg tggtcgagac ttggagattg gcgggaatta tttggttacg agtcgagggg
    11401 tttttgaact aatgtaaatc atttgttaga attgaccaga aatttctatc gtacttttac
    11461 ttttctattt gatatactga aatagaaaat atttgttgat aggggtgtta ctaatggttg
    11521 gcaataagtg gtacaaatgt gatcttcata ttcattcaac tgcgagtcag tgctttcagg
    11581 ataaaaaggt tactgctaag gaatgggtag aagaatgttt aaacaaagga ctcgattgtg
    11641 ttgcattaac tgatcacaat acaggggaaa atattgatga atataagaaa gtggctatcg
    11701 aaaatggctt agattttttt cctggagtgg aattaacgtg tggagaaaat ggaactcatt
    11761 tactagtcct atttgataaa agtgcatgta caaatgatat ctctgatttc ttaaccaaga
    11821 tgggtataaa aagagaggat tgggccagcg ctgcagcttg ctcaatgaaa tcagttttag
    11881 aagttattga ttttgctgag cacgagggct tgctcgttat tcctgcacat atagatgagt
    11941 ttaatggaat ttcttcagca aaatttggaa atctaggaaa gatatttgaa tcagaaaata
    12001 tatatgcagt acagacggtt caaaaagaat tttttgaaaa tcgctcacaa atgattccct
    12061 catctaagcg tgacactatc tatgatagtg tgaatgccag atatgatggt agagttggaa
    12121 aagatacttt ggaatcttgg tataaatcag ttgttgaggc agaaaaaaat aatatgacat
    12181 ttcttagctt ttctgataac ccacactcga aaggaaattc aaaacatgga ctttggggaa
    12241 taggcacaag atattcctat ataaaaatga aggacgaacc tgacttggga tcacttcgcg
    12301 atgctttaat gctaggcgaa actcgagttc attctgattt ttctaatttc tccattaatg
    12361 aaaatgaagt tttattggaa aaattaagct tttctgggac cactcttagc acaaaagaag
    12421 ttgtagtcga attcagcgat aatttaactt caattattgg aggaagagga acaggcaaat
    12481 cttgtattac aagattcttg gtctatgtcc ttggaaagga agcagaactt gatcaatttt
    12541 ctgaaataca aagtgattat cagaactttg cacaaattga acataatggc tctggaatat
    12601 ttttgaagga tacaattgtg aaactgaata ttttctataa ggggacaaaa tatcaaataa
    12661 taagaacaca ggatagacat tctatttatg aatttacaca agagaatggt ctggttgaaa
    12721 aagaaacaga acggttgaga atgatttccg ataaggtgag tatatactct caaaaacaaa
    12781 tttatgaaat ttcaaaaaat caaactagta tactagaatt actagatgga tataactctg
    12841 atttgatttc agagtataaa aatgagattg aaacatgtgt aaacgaaata agaaaattaa
    12901 attgggatat tgtttctgtg aaaaaagaaa tacaagataa ggcgaaggta gagcttgaaa
    12961 ttgaagattt aaaaaaacaa gtagaaaagc tatctcataa gagttataaa gatgtttatg
    13021 atgagtattc aaaagagaca gatatttata gggaactgaa acgtgatgtg gaggcactta
    13081 aggaaattcc caaaaatcta actgatagtg ttgacaaaat tgactttggc aatggtataa
    13141 aagttacaga cgaaattgat gaacaccgcg gagaattgat taagaattta gtgaccaatc
    13201 gggctaagat tcaaacaata attaatgaaa tgtcagatga aattgatagt tatagagcag
    13261 agctgaataa atcagattgg aaagttaact ataatgaggt ttcaaagagg tatcaaagag
    13321 gtatcaaaaa cttagtgaag agttatctca agatgaatta aaaatgttac aaaaccttga
    13381 ttctttatat caatctttat caaaaactga agagcgattg gaagaaatta ttcaacaaga
    13441 aataaagctt aaagattacc aagttgaggt tgactcagca atgtcaagaa tgaataaatc
    13501 atatcaaaat ttctattctg agagaagtag ttcctcgcgc gaaatttttt ctgatattac
    13561 aggaatagag gctactattc aaccaaatag ggatttcaat aattatgtta aagagctcag
    13621 agcttgtatt aataaggagg acacttttga cagtgagttt ggatctctta ttgataagct
    13681 gacaaaaaat tccattacta atcaagatat atataatgac atacttgata taagagttag
    13741 tggggaaagt agtatttttt cagataaaaa attaatcaat tctataagaa aattgaatga
    13801 tgcacaagtt cttgaattac aaacccttaa gccaaatgat ttagtagtta tccgacttga
    13861 actaaatgga aaaaaagtta gtttatcgaa tgctagtgct ggtcaaaaga catctgcaat
    13921 tctttctatg attttggcgt atggaaattc accactcata ttggatcagc ctgaagatga
    13981 tttggatagt caattaatta ataacttaat cgtaagaagt atcattacaa aaaaggaaaa
    14041 cagacaaatt ttaattgtaa ctcataatgc aaatatccca gttaatggtg attcagaatg
    14101 gttgattagc atgggagatt caaaagaaat atcggctgat cagtgcgggt cggtagatga
    14161 acccaatatc aagccaagaa tatgtactgt gatggaaggt ggcgaggatg cttttacaaa
    14221 tagagcgaaa cgctatggtt ttaaacaaat tgtagaataa cactagaggg tgagacatat
    14281 tattatgtcc gcccttttta ggaggaaaaa tgaaaaaaat aaaaagctat accagtatct
    14341 gggcagttga aaaagtcatt tacgcaatca atgattttca gttgcctttt cctgtgactt
    14401 ttaaccaaat ggcttggttc gttttatctt tactgtttgt catcgtattt gcccatgtgc
    14461 ctccactatc tatgatagag gaggtctttt taaaatactt aggcataccg gtggctgtca
    14521 cttggtttat gtcgcaaaag acgtttgatg gcaagaaacc actgggattt ttacgatcct
    14581 ttatcagcta tcacttgcga tttaaggtga cttttgcagg aaagaaagtc aaagaacaga
    14641 agaaacgatg ggatgaaccc agtactcttg taaggagtgt gaactatgta tccgattaag
    14701 tatattgaaa ataatctcgt ctttaaccaa gaaggcgagt gttttgccta ctatgaatta
    14761 gtgccttaca attactcctt tttgtcaccc gaacagaaat ttcaagtgca tgataacttt
    14821 cgccaactaa ttgcgcaaaa tcgtgaaggc aagatccatg cgttacaaat tgccacagaa
    14881 agtagtattc gcgccacaca agaacgatca aaaaaagaaa ttaccggtag actcaaagaa
    14941 gtagcgaaac aacgaattga tatacagaca gatgcattag tatccatgat tggcgatagt
    15001 cagattgatt atcgcttttt tattggcttt aaactaattg cgacagatga agaagtcaat
    15061 ctaaagagtc tcaagaaatc tttcttttct gggcttcaag aatttgtcta tggagtgaat
    15121 catcatttga tgggcgattt tgtttcctta tccaatgaag aaattcgctg ctattctaag
    15181 cttgaaaaat taatggaaag caaattggct cgaagattta aagtcaggcg agtcacaccg
    15241 agtgacctga cgtatttgat tgaacatatt tatggcgaaa aagggatacc gtttgaagaa
    15301 tacgaatttc agctaccaaa gaaaaagcta aaatcagaaa cactggtgaa acgttatgac
    15361 ttattacgtc caaatcgttg tttaattgaa gaaaaaccaa ggtgcttacg gatggaacat
    15421 gagaatcatg agtcgtacgt agcgtattta acaattaata caattgtggg ggagatggaa
    15481 tttccttcct cagaactttt ctattaccag caacaacaat ttacattccc aatcgacaca
    15541 agtatgaatg tagagattgt gacgaataaa aaggctttag ctaccgttcg taataagaaa
    15601 aaagaactga aagatttaga caatcatgct taccaatctg acaatgaaac gaattccaat
    15661 gtcttagatg ctttggattc cgtggatgaa ttggaaacca ccttggatca gtcaaaagaa
    15721 tctatgtaca aactgagtta tgtggttcga gtgagtgccg aatcagtgga tgaactaaag
    15781 cgacgctgtg atgaagtgct cgatttttat gatgatacca atgtaaaact agtccggccc
    15841 tttggcgata tgatggggct acatgaagaa ttcttaccct taagtaaacg atatatgaac
    15901 gactatattc agtatgttac gtctgatttt ttggcggggc ttggttttgg ggctactcag
    15961 atgctaggag aattagaagg gatttacttt ggctacaatg tggatacagg tcgtaatgtt
    16021 tacctgaaac cggcattggc ttcacaagga gtcaagggtt cagtaaccaa tgcgctggcc
    16081 gccgctttcc tgggttctct tggtggaggt aaatccttta gtaacaatct cttagtctac
    16141 tatgctgttt tgttcggtgg tcaagctgtg attgtcgatc caaaaggggg tgcgaaacgt
    16201 tcctacttga aaagagtagg cacagcactt cattaaatca atatttaatg tgtgttggag
    16261 aactaaacac acgaatagtc gaatgacggg gtaacgccct gaaaggctac tttaatactc
    16321 tgactggcag aaacttgagt aaacaaggct ctgtcagaag ctcggtgaag tcggcagaca
    16381 gacaccgtaa cagataatgc tgacgaaaga ttcccagagg tgggcgttgt gtcctatatg
    16441 ccggaagtct ataaaatatc tatggttaga atgtagatga gaggtctact gacgaatcct
    16501 tgaatgtaca ggtctatatc ggggctggat agaaatgtcc agtgacacaa acagtgtcgc
    16561 tactgtgggt gagtaagact ccgcatttat gaaaatccat ataacgttaa aggcgttatc
    16621 aaggtagcag gcttatagag gaaacctaag gatatatgta cagatagtgt gtttggaacg
    16681 ttgaaagctg acaacatgga gattttactg tctgtgaagt gttcacatca gaaagttatt
    16741 gtgagattag caatgatata aggtgtgtta atgtcagtga gagtggtggc acagtaccta
    16801 tgaaaccgtg ataacaagcg gtggagggat agccactagt catatgaaga atagaattac
    16861 taagaaaaat ggtatgggtt cgagtaagac taagagatgt acttgctcct gcaaacaagg
    16921 agggtacaga ccatgctaaa gaaaaacaag ctccgctata acgagtatta tgatatgcaa
    16981 catatttatg acgagttata cgcacagagc aagaacggta ataactttta taagttactt
    17041 gaaattattg gttcggaaca aaatatttgt ttagcttacc gtaatctgaa atcaaacagt
    17101 ggcagtaaaa cggctggaac ggatggaatg acgatagacg atataaaaca actcagcaat
    17161 gctgagattg ttgctaccgt tcgggaaagc cttagcaact accgccccaa atcagtcaga
    17221 cgtgttttca ttccaaaagc tgggagcgat aaaatgcgtc cattggggat tccgtgtatc
    17281 tgggacaggc tggtacaaca atgtatcctg caagtcctag agccgatatg cgaaccaaag
    17341 ttccataatc attcctatgg attccgtgcg aatcgaagtg cccaccatgc agtcagcaga
    17401 gtaacgacac tgataaattt atcaaaatat cattactgtg ttgacgtaga cattaaagga
    17461 tttttcgata atgtcaatca tggaaaacta ttaaaacaaa tctggacact tggtattcgg
    17521 gataaaagat tgatttgcat tatcagcaaa atgctgaaag ccgaaattga cggcgagggt
    17581 gtcccagaaa aaggaacgcc acaaggcggt ctgttaagtc cactgctctc cctcatagtc
    17641 cttaatgaat tagactggtg ggtaagcagt cagtgggaaa cattccagcc gaagaaccgc
    17701 agtaaaaatg ggtggcttca atacgcaaag aaatatacca aactgaaaag cggttttatt
    17761 gtacgttacg ctgatgattt taaaatcatg tgttcaacct atggagaagc acaaagattt
    17821 taccacagta ccgtagattt tcttaataaa aggctgaaat tagaaatcag tccagagaaa
    17881 tcaaaggtag taaatctgaa gaaaaattca tcagattttc tggggttcaa aatcaaagtc
    17941 attccaaaag gaagaacgaa acacggttat gttgccaaaa cagatatgaa ccaaaaagca
    18001 ttaaagaaag cgaaaacaaa tctaaagtta aaggtaaaag atatagccag acatacgact
    18061 ggcttcaata tcagcagata taatctgaca gttatcggta tgcagaatta ctactgcata
    18121 gcaactaacg tatataacaa cctgacagaa gtaagttacg ctctcttacc gaccatacga
    18181 atccgtttaa ggaacattgc aaaatcagtt ccatttgaaa gtacaagttc agaatttcag
    18241 agcaggacaa aaggaatcag accaaaaaca aaaatagtga tgattgcgga taatccgcta
    18301 ttgccgattc aaggagtaca acacaaaaat cctatgaatt tctcgcaaga tatttgtaat
    18361 tttacaaagc aagggagaaa caaggtgcat gaggacgttg tagttgtaac aaaagaagaa
    18421 atccgagcgt tgttggaaaa tgaaaaccct gccgatagcg tagagtttaa cgacaatcgt
    18481 atttcggcat atatcgcaca acaaggcaac tgctatgtaa tgaatcgtcg aggaactccg
    18541 tccactctga tatgtattca caaatcagac aaaggagata accttgacag atattctaat
    18601 ctggcatttg tggaaattcc cattgcaaag gcaatcctaa cagaatcagc agacgaagca
    18661 aaatcactgt taaaaggtta tgttcttaat agtcagcaga agaaaaaact gaacagaata
    18721 cggacgaatt acggatacca gacaataact gggaaataac ttataaatct atttggatat
    18781 gatggaacgc cgtatgaagc gaaagtttca tgtacggtgt gaagcggggg aaaacttgga
    18841 gataacatca aaaagttacc tatcgctata gcgaggaggg tggaaagaaa ccttgccaga
    18901 aattgcggat gaaatcagta tcctcaactt gacgagtaag tcagaaaatc aaggtctgct
    18961 tgatccttat gtgattatga agaaaaagaa agactcggaa agtttagcaa ttgatattct
    19021 gactttttta acaggaattt caagtcgaga cggggagaag tttcctgtgt tacgtaaagc
    19081 cattcgaacg gtgactcaaa gtgatcaacg ggggttatta caagttatta acgaattgcg
    19141 aaaagacggg tcaccaatcg cggaaaatat cgccgatcat atcgagtcct ttaccgatta
    19201 tgattttgct catttgcttt tctcagacgg ctcgattacc caatcgatta gtttagataa
    19261 acagctaaat atcattcaag tggcggattt ggttttaccg gatgcagaaa cgacttttga
    19321 agaatatacc accatggaat tgcttagtgt ttcgatgcta atcgtcattt ccacttttgc
    19381 cttggacttt atccatagta accgagaaat cttcaaaatt gtcgatttgg atgaggcttg
    19441 gagttttctt caagtggctc aaggaaaaac gttatcgaat aagcttgttc gtgctggtcg
    19501 ggccatgaat gctggggtct atttcgtcac tcaaaatagc gacgatttat tagatgaacg
    19561 tttaaaaaat aatatcgggt tgaagtttgc ttttcgcagt accgatattc atgaaatcaa
    19621 gaaaaccttg gaattctttg gtttggatca ggaggatcaa agcaatcaaa aacgcctgcg
    19681 agatttggaa aacg
//
LOCUS       DAZKLA010000047        18593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000047 DAZKLA010000000
VERSION     DAZKLA010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.047"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(172..1464)
                     /locus_tag="ACG85N_001409"
     CDS             complement(172..1464)
                     /locus_tag="ACG85N_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0224185.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(1461..2084)
                     /locus_tag="ACG85N_001410"
     CDS             complement(1461..2084)
                     /locus_tag="ACG85N_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224186.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(2087..2923)
                     /locus_tag="ACG85N_001411"
     CDS             complement(2087..2923)
                     /locus_tag="ACG85N_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0224187.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(2940..3827)
                     /locus_tag="ACG85N_001412"
     CDS             complement(2940..3827)
                     /locus_tag="ACG85N_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0224188.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(3843..5126)
                     /locus_tag="ACG85N_001413"
     CDS             complement(3843..5126)
                     /locus_tag="ACG85N_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0224189.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(5809..6492)
                     /locus_tag="ACG85N_001414"
     CDS             complement(5809..6492)
                     /locus_tag="ACG85N_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0224190.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(6510..7127)
                     /locus_tag="ACG85N_001415"
     CDS             complement(6510..7127)
                     /locus_tag="ACG85N_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0224191.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(7137..7961)
                     /locus_tag="ACG85N_001416"
     CDS             complement(7137..7961)
                     /locus_tag="ACG85N_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFQ0224192.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(7954..8514)
                     /locus_tag="ACG85N_001417"
     CDS             complement(7954..8514)
                     /locus_tag="ACG85N_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224193.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            8688..9644
                     /locus_tag="ACG85N_001418"
     CDS             8688..9644
                     /locus_tag="ACG85N_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0224194.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(9949..10905)
                     /locus_tag="ACG85N_001419"
     CDS             complement(9949..10905)
                     /locus_tag="ACG85N_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFQ0224195.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            11009..11611
                     /locus_tag="ACG85N_001420"
     CDS             11009..11611
                     /locus_tag="ACG85N_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0224196.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(11699..13198)
                     /locus_tag="ACG85N_001421"
     CDS             complement(11699..13198)
                     /locus_tag="ACG85N_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFQ0224197.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(13214..13738)
                     /locus_tag="ACG85N_001422"
     CDS             complement(13214..13738)
                     /locus_tag="ACG85N_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFQ0224198.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            14036..14785
                     /locus_tag="ACG85N_001423"
     CDS             14036..14785
                     /locus_tag="ACG85N_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0224199.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            14776..15561
                     /locus_tag="ACG85N_001424"
     CDS             14776..15561
                     /locus_tag="ACG85N_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0224200.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            15530..16246
                     /locus_tag="ACG85N_001425"
     CDS             15530..16246
                     /locus_tag="ACG85N_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0224201.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(16348..17367)
                     /locus_tag="ACG85N_001426"
     CDS             complement(16348..17367)
                     /locus_tag="ACG85N_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFQ0224202.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(17386..18387)
                     /locus_tag="ACG85N_001427"
     CDS             complement(17386..18387)
                     /locus_tag="ACG85N_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFQ0224203.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQSMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         5576 a         3914 c         3257 g         5846 t
ORIGIN      
        1 aatagttatt tttattttct cctatctaaa aaagcagccc acataaaaag aaggcatgac
       61 aaaaagcatg tcatagcccc tttttccgaa taaatagtgt tcaaaagaca gcctatcggc
      121 aataaggcaa gattttataa actacgggaa gtaatttccg taaatctatt cttattcttt
      181 taagctgaca agctcgtcaa tcactactgc ttttcctgaa gcaatagaat gatttgctgc
      241 gattcctgtc aaaatcgaca aaataccatc tacgtgagtt gctgcccgat tccacggatc
      301 ttcttctttt tcttcaccaa atagatcatc taataaacgc ggatctcctc cgccatgtcc
      361 gcccgcttct tgttcgacga ttacctcata tggctcaccg aacatcggat agacatggat
      421 ggactgaaga acggctgctc cttcatcctt tttatctcca cctgcattga tgtaagactt
      481 ttcgatcact ttcatttcga tccgtccttt gctgccattg aaagaaacat tgaatccttc
      541 ccacggcata tacgcgttta gcgaatacgt caaaatcgca ccagtttcat atttgaccgt
      601 tacgcctagt gtatcttcaa tagagatatc atcggaaaat acactgcggt cacgaatata
      661 tccactgtct ttttctgctt ctaaatacat ttctcgcaat tcctcattgg ctgacaggtc
      721 tatagcaaat ggatcagctt tagctagttc gcttccatag catcgactgt aaaatatttc
      781 tactccgcgt ttctccgcat tatgcatccc ataaaagttt aatccaccga atgcaaatac
      841 cttttgggga cggctgttca accaaaaatt cactagatca aaatgatggg tagatttatg
      901 aacaagaagc ccgccgctat tgtgcttatt tcgatgccat cttctgaaat aatctgcccc
      961 gtgttgcgta tttaacagcc attcgaagtg gatagaaaac acatcaccaa tcacatcact
     1021 ttctatcagt tcctttatct tcgtgtgata cggtgcataa cgataattaa aagtgactcg
     1081 caggcttctt cccgtctgtt tttggaccgt tattatttct tgagcttttt cctcatcgat
     1141 agtcagaggt ttttcgcaaa taacatcata tccttttttc attgcacgaa tgatataatc
     1201 atgatgcgtt cgatctactg acgtaataat gacttcagcc ggccgtacgt catccagtag
     1261 ttgctcaaat tgtgatggca tacatgtctt tacttttttc tgtccatact tgttcacaag
     1321 tatccgattt gcataatcca tccttgtttg agatacatca caaaaaccga ctagttcaca
     1381 agtttgatga tacgtagagg caattgcctc ataaaaaaaa cgcgctctcc caccaagtcc
     1441 gacttgtaca tattttttct tcatttaaag tcattctcct tttcttttaa gcaaaatact
     1501 gtgacaaatg gcagccctgg atacagtaat gcagtgagta aaaaatcatg ctgtatacac
     1561 cagaacagtc catacaaaag gcataaatac cacagaaacc ccttgacgtt ttttgacaac
     1621 agccagatat tccttttgat caaataaaca agactatctg ctttatcttc tttgcagata
     1681 tcaattagcg tactaaaaaa actatgggat acaaaaagca acagacacaa tagaaaagga
     1741 atcagatacc aagctccaat ctttcccact aataaatacc aatcaactgc aaaaataatg
     1801 acaaacagct gaaacaacgt aatgatgaaa cacatccgtt tgatttttcc tttcctccaa
     1861 catacctttt tttctgccaa gtaatggaca gatagataat aagaaggata catcatcaaa
     1921 aaataaatca gatagacaac acgagcagac agcactaatc ctaaagtgaa caaacatcct
     1981 agaactaaag cgaaaagagc attactgata agactggtta aaaaaatccg acagacatag
     2041 tagttgagct tgtaaagaaa catctcttct attggctttt tcatattcag cccttcagac
     2101 cgcttgtact gatcccctgg acaagatatt tattgaagaa taagaaaatc agaaaaaccg
     2161 gtagcagcga caacgtagac atcgcaaaca tactcgcgta agtcgtacta gatccagcgt
     2221 ctgcaaataa tttgatggca atcgataccg tgtaagatct cggcttgttt aaataaatca
     2281 atggtcccat gaagtcgtcc catttccaat aaaactggat gatgatcgtc gtgatgatcg
     2341 aaggtttcag caaaggaaaa ataattttgg taaagacact atacttgctg catccgtcaa
     2401 taatcgctga ttcatctaac tctttgggga tgctgaccat gaattgcatg atctggtaaa
     2461 tgaaaaatgc ttgtccaaaa aaatgtggta aaatcagcgg cacatacgtc ccaaccaaat
     2521 tcagccgatt gtaaataata aactgcggaa tcaaaatcac ttgtgcagga atcatcatcg
     2581 ttactaacat gacgacgaac cagaatcttt ttcctttgaa atcgattctc gataatgcgt
     2641 aagcaataca agtcgagctc agaactgtcc ctatcgtcga aactgcagag atgacaaacg
     2701 aattcctaaa gaaagtaaaa aatgaaattc cagcaaaccc cgcccatcct tttgcgtaat
     2761 tttcccattg aaaagtctct ggaatcaaat tcagcgaacc actcataatc tcttgatccg
     2821 ttttaaaaga acctgcgatc atccataaga ccggataaat catgactaga cctaatagca
     2881 aaataaaacc gtattgaaac catttcgtta ctgtattatt catttcttcc tcctctcatt
     2941 cacacaacga cccatttttt tgatgatttg aaaatcagaa aggtgacagc tcccataatc
     3001 aacagcatga tccacgaaat agcactagca taccccatag aattaaaaga aaaagcttgg
     3061 ttatacacat aaagagcata aaagttcgtg gcatcattcg gaccaccttg ggtaatgata
     3121 aatgcctgcg taaaagtcat aaacgccgcg attgtctgca taaccaagtt atacaaaatg
     3181 atcggcgaaa gaagaggtaa cgtgatcttg taaaacgtct gccatttgtt cgctccatcg
     3241 atctctgccg cttcataata cgagcctggg atctctttga tacctgcagc aaagatgatc
     3301 attgccgaac cgaattgcca agcagacatc aaaacaagag ggatcatgac cagtttttga
     3361 tcgctaaacc aggaaagcgc aggtgcgcct aatcgttcca ggaaaatatt cactaacccg
     3421 tttttagaaa acaactcccg ccaaactaga gcgatagaaa tactgccacc gatcaaacta
     3481 ggcacataat acaaagaacg aaagaaagtc gttcctttga tattcctcgt taaaagataa
     3541 gcaatcaata gaccaaagat cagtttcaca ggtacagatg caaatacata gagaaaagtg
     3601 actcggatgg agttcaaaaa acgggcatca ctcagcatct ttatataatt atccaagccg
     3661 atccatgaga ctggatttcc caaacgataa tcggtgaacg aataataaag tgacaagatc
     3721 atcggtatca ctgttatcaa tagaaatcct aggatgaatg gagaagcaaa tacgtaaccg
     3781 accacattgt cttgatccaa gaaccgtttt agtttttctt ttttcatgcc ttcctcctgc
     3841 tgctatccta gaatgtttga cgcaaactca taaagactct ctgctgcttc ttctgctgtt
     3901 acttcttcat aaatgacttt ttcaactaaa agattgtatt ggtcttgaat ttccggattc
     3961 aaagcactat caaagacatt cacttcgccg ttgtccatat cggaaatcat ttctaaataa
     4021 tcaaatgtcg cttgcaaagt tgcatcatct gcacgagcga gctgttctcg aatattcgac
     4081 atgattggta caccacgttc tccttttaaa atttcattgg cttcttcact gttttgaaag
     4141 aaatcaataa acttagcagc ttcttcttta tgggctgagc tttttgccat actcagcatt
     4201 tgcgaagaac gtaaagggat accagattct ccatctgctt ttctgcgagg tggattaatc
     4261 aactgaatct ccatatcttc aggagcccct gacaatattt ctgtcaactg gttacttgat
     4321 agaaacacca ttgccgcttc tctatcccta accgcctgaa aagacagatc cgtcgtactt
     4381 gcagctccac cacctggttc tggatgggcc cctttgtcta ccagattttt acgcagctgg
     4441 aaaaaatctg ttagatagtc tggattttca aatcccaagc tgttagatag atcttcccga
     4501 ttataaaaat tcaatccttt ctcaacttgt ggtatttgaa taatacagcc agctataaaa
     4561 tcttcgaatc ttgaaatagc atagattcct aattcttcag tgatttgata gctgatttgt
     4621 tcaaattcat cccatgtcca atttgaagct ggttcaggga tatttaattg ttcaaatata
     4681 tttttattgt aagcaatcgc aaacgaattc accccgttag acagtccaac taattgcccg
     4741 tcgtaagtcg tcgttgctaa ataggattca tctgcattag aaatatcaat tatcccttgc
     4801 tccacatatt catctaatgg ttcaatctga ttgatgtatt gaggaaattg attgttcagt
     4861 tggaaaatgt cccagatatc atctgatgct gctagtgtac ctaattttgt gtaatatcct
     4921 tcataatcat aaaattcata atcgatcgtc acatggggat ttttttcttc ataaagctcg
     4981 attactttta ctgttgcatc gtgtcggact tgcgaacccc accaagccat gttcagcgtg
     5041 atttttttgt catcttcttg cccacaagcc gtcaagaaga ataacataac acttaaagct
     5101 aagatgattc tcattctttt cttcattctc tgcacctcta cttccattgt tatttttcaa
     5161 tatactgtat ttttatacag aacatattta ttaaacgatc tttttcaagg ggaataaaaa
     5221 ccaagacttg ccttatcgtt ctgcctagga tgaacaactc aatactttca agaaaaatta
     5281 gatagtgctg attcttcttt catcgctaaa aacacttctt gcttcaatga tgggaggata
     5341 attacgcaaa gaaaaaagga tatcagacaa ctgcccaata tccttgacat agctaaataa
     5401 gcatgcttct tcgtgtgcgc tcttccctaa aaaacagaaa aataactagt aattctgtat
     5461 gaaattaggt acaatataca cgtcgatgca caagcattga ctatggcaac ttgattggtc
     5521 agttcccgct gacttttcaa gccccgacaa tttagctgct actaaattgt cgggtgcctt
     5581 ttttagtttt tattcgtttt tcattctata gaaagcgctt caataaatca agacattttt
     5641 ttgttagaat gctaataata acaaaaggtt ttaactcttc ttatgtacat tacaggtaaa
     5701 aaaacaaaaa aatttctctt ttttttgaat tctgaacaaa tagtactgga gtacaaaaga
     5761 cgaggaagag ccactatctg ctcttcctcg tcttttgatt ctcacctgtt attcatgatt
     5821 ctttttctca gtttcccgat atttttccat tttacgacta aaaagctctc cgctacttgg
     5881 tggcgtatat gtgatggcat ttgctcctgc ttgaatggtc tcagcaatac tttcttccgt
     5941 tggaccacct gtcgcaatga taggaagttc aggatatttt ttacgaaaat ggcgtactgc
     6001 ttctgcagtt tctttacctg cactgatatt gataatcgaa ataccagctg ctaacttttc
     6061 atctatttct gtatatttcg acgttaccgt actgataact ggaatatcaa ctactcgaca
     6121 aacttctttt actgtctcta aaggcgtagg accgttcaaa acgaccccta acgaaccttg
     6181 cgcttctgcg aacatgctca tgtaggaaga acgagcacct tgtgtcaaac caccacctac
     6241 accggagaaa actggaatgt cagctgcctc aataatgctt ttagtaatcg ctggatgagg
     6301 tgtaaaagga tacacagcaa ttactgcatt cgcatttgta ttacgtatga tcgcaatatc
     6361 cgtagtaaag atgatcgaac ggatcaattt tccaaatatc ttgatcccgc ttgcttctaa
     6421 aatgatttcc ggtactctta cgatatcttt tctcaattcc gtcatgattt ctggcgtaaa
     6481 ttgttctgcc atcagccact tctcctttgc tatctccggt catactgatt caattgatac
     6541 gtctcgatga cctggatgat tttttgcgca taaccggggt ctgtcgcata acctgcatct
     6601 tgcaatgctt gtgcggcttc ctgatagttg gtggccgtaa tcactttttc atacagcgca
     6661 ggattccaat ctactccttg gacgaacagt tgtgtatggt catccatgga ctgttcccat
     6721 gaatcataca ctttgaagtc tccttggata gttatccatt cttcgttaac aaattccttt
     6781 gtttccaggc ttactttctt ctgatcccca taagccttga tcccgaaaag attattgtat
     6841 ttgctagcca aggtactttt tccccaattt gattctaata tagcttgtcc taaaatgata
     6901 ctaggaagca ctccataacc atcttgtagt tcttgggcat gcggaacaag ccgttcaata
     6961 aattgctgat gggattgcgc ttcattttct tgctgattat cagtaaaagg agaagacaga
     7021 ttcttcaagg aaaaaacaaa ggctaagagt atcactgcta caccggcaac cactgctggc
     7081 caacggatat tcttgcgctt ttgctttttc cacttttttt tcgccatgat caattcctac
     7141 ttttcattta attcattcag atattcttct agcgtcaatt ggtgtttggt gatatactca
     7201 gcgttttctt taccgacata acgtaagtgc caaggttcat atgtgatctt agttatatct
     7261 tcgtgtcctt ttggataacg aacgataaat ccatactgcg cagcattatc tgcgatccat
     7321 ttagctccag gctgatctcc gtaacttgca tccaatacag tagaaggata actgttgtac
     7381 cagttttgat cgacgacatc gactgccaga cctgtgtggt gttcgctgta tcctggctcc
     7441 gtaatcgttt ctttagtaat ctttgtcgcc tcctcttctg atacaccttg acgactcatc
     7501 acatcttgga cattttgttc gaaaacttgt tgttgggatg atacagaccg gtaagctgag
     7561 accatcacta atggaaatcc agctgcttca gcagccttgg caaatgcttc atattctgga
     7621 gcaatgcgtt tgtcgatcaa gtatccgtta cttaatgatg ccaattgagt agactcatca
     7681 atttcttttt ctaatttgtg atctggacca accaaaacta gcttccaatc gtctgtttta
     7741 acatctggca attgtttgga cgtacgatct tttgtttctt tgtcctttac cttgctgctg
     7801 gttgtttgac tgttcttttc tttactagca gttgttttgg tgcttgttcg ttcgactgct
     7861 tgattgccgg cttgaacatg cgaatcatca tcgctaaaaa taacatagcc cattgctgcg
     7921 atcataatca caactgctcc aaatcctaaa aatttattca cttgaaaaat cctttcgttg
     7981 ttcttcacgt tccccattcg cctcatgaac gtaagtttct tccagatttt cattcaccca
     8041 atcgagaagt tcgatttttt ctccttcgat ctgtttttga acatctgctg gcagacgaaa
     8101 attcaaaata tcatcatagc cccattcatc atattgaata cttactttca agtcaaggta
     8161 tctaccagcc tgctgattat ttttcaaagg gatcagctcg acttttgctg ctttgctgct
     8221 aagccatttt tgcgcgatgc gtgttttgat ttgcttgtac tctgcaacgg caacgtttcg
     8281 tttttcggga aaaataatcg tttgacgcaa aatcttttgc gttagcatcc attcataatc
     8341 agtgaaaaga ttttttactt tctgcatcgc ttcagatggc agatttttct cgccatcttt
     8401 ccatttttgc cattgttctg gactgatcag caagtattca tctaaaaaat ttttttcagt
     8461 ttcaaactgt tctaataata cactcataat aagatcaata tatacttgat gcacgtttat
     8521 cccctccttt taactattct acttaagttt tgatcaaaaa gcacctctta aatcgctgct
     8581 ttcatttttt ttaatgtttc aagtcctctc ctctttattc ttcgtgattt ttgttataat
     8641 aatagacaga caagaaactc aatccaacta tgaaggagcg ttttcagatg aaaaaatatc
     8701 aatggggaat cgttggtctt ggtaccatcg cacatgagtt tgccgaaagc ttcaatcaag
     8761 agacgagcga actaacagca gttgcttcca gaacttcaga aaaagcggaa aacttcgctc
     8821 accgttacaa tattccaaaa gcttacggaa gctatcaaga aatgttagac gatgctgaaa
     8881 tcgacatcgt gtacattgct gtaccaaata gacagcatat cgaccatatc ttggcagcat
     8941 tggaagcagg caaacacgtt ctatgtgaaa aagccatcac gatgaataaa aaagaactgg
     9001 ctgacgctat gcgcttagca gaagaaaaga atttgatctt agcagaagcg atgacaatct
     9061 tcaacatgcc actttatcag cagttacgct caataatgga tacaggaaaa ctgggggctt
     9121 taaaaatgat ccaagcacct ttcggcagtt acaaagaacc agaccctaaa aaccggttct
     9181 tcaatccaga attagctgga ggagctctgt tggatatcgg tacgtatgct gtatctttcg
     9241 ctcgtttctt tttgagctct caacctgaag tagttgcctc tacaatggtt ccttttgaaa
     9301 caggcgtgga cgaacaatcc gtcactatct tgcgtaacaa agaaaatgaa cttgctgctg
     9361 tcagcttgac ttttcaggca aaaatgccaa aagtcggtat cgttgccttt gagaacggtt
     9421 atgtgacgat tgctgattac ccgcgtgctg atcgtgcgga gatcttattt acagatggta
     9481 caaaggaatt catcgaaagc gggaatacat ctgaagcaat gaattacgaa ataaagaata
     9541 tggtacacat gattgaaggt gacttgccca atcgctctct cttcttgaca aaagatgtca
     9601 tcgaaatctt ggatcaaatg caagttctat ggaaaaatat gtaattttaa aaggttgtga
     9661 aaaagctgtt ctgcatcaag taataagaac agccaaacaa aaatagctcc tcctattttt
     9721 cgtttggctg gacttattac cgcagaggaa agcttttgaa caccgtttaa gaaagaggtt
     9781 gtgagcctac cgttcagctc cgagtcacaa ggagcaattt caaaaaacag cttctcatgt
     9841 ttgttgaata ttgtgacttg tgaccgagga gttgacagga gaacaccgtt taaagaagag
     9901 gttgtgaaat cagctttttg acctgaatat tagctgaatt cacaacctct atttttggat
     9961 tttttgttgg gagaattctg ccttgatttc aataaaactt ttaaaagact gcaaaaactg
    10021 gaacaattct gctcgatcct cgaattcagg gcgactagtc ggcagaggca tttgccgata
    10081 caatacataa acttcttcaa tacgtgttaa aatttgtctg ccatcatttg actctgaaaa
    10141 tgtttctgca gtataaatca ataaaccata gatatgtttt ccataataag ctggttgctg
    10201 gattctctct agattttcca gcatgtcttt aatcacattg gcctgtgctc ttctcataga
    10261 gaagtaagtt tcgtagtata gcggctgtct gagccagtag ttttcttgat gttctctggc
    10321 atcttcttgg ctttcgcgaa tgaatgtcag taaatgttcg catttgatct gcacatcttc
    10381 tttattttct gataaaagaa attctgccat ttgtctcaaa atatttcgaa attctttttc
    10441 gatctgtatt tggttttcct tcagctgctt cgtacgatct ggcatgtaaa catttgcaag
    10501 caaagcaaat actaatccaa tcgccattaa tcccgcttca ttgccgatca gctgccaaga
    10561 catgttttcc tccactaaat aatgagtgac caaaaccgaa tttactacta tcccttcggt
    10621 taatttgaag cgagaggcaa gcgggatgaa caggaataaa aaaagactga agccaaaaat
    10681 agtataccca acagcaaaaa aactgaacag tgctgttgcc gtagctaata taaaggctgc
    10741 gattcgattc atgcccgtac gcaatgtaga gcgcttggta tttcctacac ttaataatgc
    10801 aatgatccct gcagctggcc aatacaacag cgataagcta cccgctgtga ccatcgaaaa
    10861 gaagacactg atcactgttt tgactgttcg cattcctata cgcatttact tccccctttt
    10921 ttattttaat tatacttgaa taatgaaaaa ctagctatca gacaaaagtt taggtatgat
    10981 agtaaaaaga ccaaaaagga gaatctctat gcaatttggc cgttttcgac taggtatgcg
    11041 tacgatcaaa agtgccttag ctgttttttt atgtatcttg ttttttcatt tgactcaacg
    11101 aggaatcccg atgattgctg cattatctgc tgttttttct ttgcgtcagg acttgactac
    11161 aaccgtttct tttggaaagt ctaggatcat cggcaataca ttaggtggta cattagctat
    11221 catttatttt tttgtcaaaa gctattttca caatgatttt ttggtagaat tgatcttgct
    11281 tcctattcta gttattattg tgatcgtcgt gtcagatggt attaacaata attccggtat
    11341 catttccgca atagctacgt tgttgctgat tgcgttgagc atccctcaag gcgaatccgc
    11401 tttgtatgct atccaacgtg tactagatac ttttataggg acattcattg caatcggtat
    11461 caatttcttt cttcgtcctc ctgaaacaga aaagaaggaa gaactatcag aagatttggt
    11521 cgaactccaa aagaaagaac gattgctaag aacaaattta caagagatag aagctaagat
    11581 ccaaaaacaa aagaaaaata gcgaggacta attttctttt gcaataaaat aaaaaaatgg
    11641 agagaagaac tttaaaaagt cttcctccat ttttgattga acggtgaagt gtactacttt
    11701 attcttcctt catcgagttt tgttctgcct cttttggcgc ttcatacata tttacttggc
    11761 tagtagtccc acgtgtcgac aaattctcta cgatttcctt caagtcaagt cctgttgtcg
    11821 cttttagtgt ttcttgagta gaagaaagca aatttgtggc ataatttgtc actcggttcg
    11881 ctccactggt ttctcctgaa ctgcttcctg tatcgacaac agagattttt tcgatatttc
    11941 ctagcggctg tgctgcttcg cgcatcaatt gcggtagcat atcgataacc atactcaaga
    12001 cagctgcttc gccatattcc ttgaaggcat tggcgatctt ttgttttgct tcggcttctg
    12061 cttttccttt tgctagtgct gcttcggctt ctgcttgtcc agctaaacgt gtcttattgg
    12121 cttccgcttc tgccagtgct tccacacgga atcgttctgc ttcggcttca gtcacttctt
    12181 tgactttttg agcttgtgct tcttgttctt tagcgtaccg atcggcatct gcttttttct
    12241 tcacttccga atcatattgc ttttcacgac gtgtgatttc tttttcttcc agttcgattt
    12301 gtttttgacg ttctacgatt ttaatctgca tttcctgttc gattacttgt tgttgtgctc
    12361 gggcactttc taaattgtag gcttggtctg cttttgcttt agcaacatct tgttcttgtt
    12421 tgtaggttgc taatttcagt tctttttctt ttaatgattc tgcgatctct gtttgtcgct
    12481 gtaattcagc tgcttgtgat tctttttctg cttgagcttt tttgatacgc gtttctttta
    12541 gagcttccgc ttctgctata tctgcatctc ttttgacttg ggcgattctt ggtttcccta
    12601 aagagtcaag ataaccgttt ttgtctctga cctctttgat agtgaaagaa acgatgatca
    12661 accccatttt tgcaaggtct acagaagcga cttcttgtac gctttgactg aacttatcgc
    12721 gattttgata gatttcttct acagtcatag aaccaagaat cgagcgcaag tgtccttcta
    12781 atacttctct tgcttcattt tccagttctt ctcttgtctt gcctaaaaac tgttctgctg
    12841 ctgtagcaat ctcttctaca gatgaaccga ttttgatgat cgaagtacca tcacacatca
    12901 ccggtacgcc ttgttccgta tacacttcag gggtggagac gtccaattta ctcgataata
    12961 gactgatccg attcgaacgt tgaaaaactg gcaagacgaa tgcgccacct ccacgcacaa
    13021 ttttgatttt gttattgctt tcatcaatgt gcacattttt gcttcctaaa taacttccac
    13081 tgatgatcaa cgcttcatcc ggttttgctg tctggtactt cgctacaaag acgatcagca
    13141 acatcagtaa aatcaaagct gcgattgcga tcgcgataaa aacaactggt atttccattt
    13201 aacacacgct cctttaagct tttatcatgc tttcataagg catgacataa cagattcttt
    13261 ccttgatctc aatgatcaat actttcgttc cccgctcagc tgtctgttct tgggggctgt
    13321 aaaaacaagc tggacgacta atggaaccgg taacactttt caactgaatt tctcccatac
    13381 ctcgtacagg gatcggtgtg atcacaacag ctacttgtcc ttccagagtt ttttcagaag
    13441 acgacatcgt tgattcggca tgtttcatcg gcatcaatat atagaaatta agcaaaaata
    13501 ccaacactat cgatatgcct ccactagcaa taaataaaaa taaagatgat tgatctgtta
    13561 aacgctcacc tagaaaacca aataagcttg tgaaagttaa ccaagggata ataagcatcg
    13621 gatcaagcgg gccatcaaaa tcgaatacat ctccaaaaat aatcaatagg actgccaatc
    13681 ctgcacaaat cagcaatgta taccaataaa tcgtctcaag tgcgattccc cctatcatat
    13741 acacgcctcc ttccatttct acgtctatca taccaatgtt tgaaaattct gtatagcttc
    13801 ttttaccgac tcagtggtgt tttttatagg aaggttgtgt ttatcgttga caatttgtta
    13861 aatttttgtt ttcatgttaa aattttcaca ataaattctt tcgaggtagc ctatgtttcc
    13921 tttttttaag aaaaaaccat taaagctcca gaacacgatc atccttgtcg tttgtttctc
    13981 cacattcgtt tctttgattg tttctgccat attgatcagg aattttattc tttcgatgaa
    14041 ccgatctagt gtgtcagata aaatctctag cattgctaaa atcgttgcca atgataaact
    14101 catccaaaaa aagctgcaga ctggtgaccc gacacatgcg attcaaggaa taacaaatga
    14161 aatcatgatt gcatcgaatt tagattttat cgttgtcatg gaccctgaag gcattcgata
    14221 ttctcacgca gacccaagtt acatcggtct tcctttctca agtcatgaag atgtgaaaaa
    14281 agtgattgga ggacaagaat ttttctctga aaaagaagga atgttagggt tcggcttccg
    14341 ctatttcgtc cctatttatt atgatcaaga gattattggt gcagtttgtg ttggcttaac
    14401 aaacaacacg attgacaatc aagtcaaaga tggacaaaaa atgattgttt tttcattgat
    14461 tattggactt agcgtaggca tcataggcgc cgtttttctt gcccaaaaaa tgaaaaaagt
    14521 actgcttgga cttgaaccac aagaaatagc agaacagtta agtgaaaaaa cgattatcga
    14581 aaatgaagta gatgaaggaa tcttagccat ctcttctgat aaaactatcc tcttgatcaa
    14641 tacacctgca aaaaacattt tctaccaaac agagcagcta ttggatttcg aagtaggaaa
    14701 aaaatcagtg ataatctata tgacgttttg tttaaagaga cgattgagca tcaaacgacc
    14761 gttcggactc aatccatgta tttaaatggg atagaaacta tttctagcgt tacacctctt
    14821 tttagcaatg accgtttttt aggagcagtc gcaactttac gaaatcgatc agaagtaatg
    14881 aaattaatgg aggagcttgg ggggacgaaa caatatataa actccatgag agctcaaacc
    14941 catgaattca tgaataaact tcatgtcatt tctggtttga ttgatttgaa gaagtatgaa
    15001 gaagtcaccc attttatcca gcagctaaat caaaattata aaaaagagat tggccaagtc
    15061 tcaagtctga tcaaagtacc tgcaattgcc ggttttttat taggcaaaat aaacgaagcc
    15121 aaagaacaat ctgttgaaat gctgttagaa agcgattctt ttatccctaa aatggagatg
    15181 gatgactcca tccattcact tttgcaagtg ttagggaact tacttgataa tgcaaaagaa
    15241 gccgtgctac aaaaacatga aagaaacgga caggtccgga ttcatttaaa ctatgacaca
    15301 gaagctgaag tattcatcgt gaccgtctgc gatgacggtg tcgggatatc agaagaaatc
    15361 tcagaaaaaa tatttacgct aggtttttcg acgaagggaa tcaatcgtgg gtttggattg
    15421 aatctcatcc attcaatcgt caaacgccac ggtggatttt tagaattttc caaacaaaca
    15481 aatggcggta caatcgtcta tctagaatta ccaaaaaagg aagatcaaaa tgatgacaaa
    15541 tgtcctgata atcgaagatg atccaatggt tgcagaattg aatcgtactt ttgtggaaag
    15601 catccctaat atgaacgtaa tcgaaagttt cagaaatgta gaagaaggaa aaaaatattt
    15661 cagacaaaaa aacgcagatc tgctgctgct tgatgtatac atgcctggcg aaagtggtat
    15721 ccatttttta tcttggctta gacagcaagg aaatgatatc ccagtgattt tgatcactgc
    15781 tgcagatgat atcgaaacca taaagaatgt gatccgctac ggtgccttag attatttgat
    15841 caaaccgttc actttgcaac gttttcagct ggcgatcgaa aagtatcttg ctttctctca
    15901 gacaatccaa acgactgaaa aagccgatca gaaattgatc gatcaagtat tcaaccacac
    15961 cactcccatt cacgaaatac catcattcat cgatttgcct aaaggtctgt caaaactgac
    16021 tttaaaaaaa ataagtgcat ttattctaaa agaagatact attttttcaa caaaagaact
    16081 ggctcaaaaa agctcactgt cacgtatatc cgtcaagaaa tatttgaatt ttttagtaga
    16141 acaagatata ttgaacgaaa gaatcaaata tttggaaatc ggccggccgc taacctgtta
    16201 tgaattgaca aagaatgggc ggaaaaagct cacagattac tgttgagtca tcgaattatc
    16261 ccatacaaaa tagtccaagt atacagaagt aacaactgtt tacttggact atttttaaac
    16321 attttattga cgaatatcca tgttttctta tcttaattga cgggctttag gaagagatac
    16381 ctcatttttc gtttttccag tttctattat ttcacgaaag ttctcgaaac ttgtcgaaat
    16441 catatttgat aatgcctcat ctgtatttga acctacatgc ggggttacaa gaactctagg
    16501 gtacatttct actaattgct ggatagcagg gtctggaatc gtctcccatg gcttgaagga
    16561 tcggaaaaaa acctctgttt catttgcaaa aacatcggtt gcaaaaccag ctaaatgatt
    16621 gcttttcaaa gctcttaaaa tagcttcatt atcttgaagc tctccacggg ctgtattaat
    16681 caagatgcta ccttttttca tttgagctaa aaatccttca ttgatcattt gatcgttttc
    16741 acctgggata tgaggtacat gaatactgac aatatcgctt ttggctaata actcttcaag
    16801 cgagtggaac gtcaatactt ttttagcagc ttctgtctga tgaatatcgt agccgatcac
    16861 tttggctcct agtcccttaa acaaaacagc ttccgtcaac cctatcctgc ctgttcctat
    16921 gatccctact gtgcaatttc tgatttcacg actaaacatt tgctcatcca ctcgaaaatc
    16981 tttgaaagat gttcggatcg tcgtatacgc agtatttctc aaaagcatca tggcaaaagt
    17041 caaagataac tctgctatcg catttggcga atacgatggt acacgggcta ctgtcatacc
    17101 aaaatcagct gccgcttgta gatcgatatg attgactccc acagtccgtg taaaaacata
    17161 acgaatccca tattcctgca tttttgcaag attggtctca tctgctatac aatttcccct
    17221 caacaataca gcatcatggt cctttgctga cacgatattg tcatgtgtca acagttcttc
    17281 ataaagactg agatcataat ggtaaatatt caatttttca aaaaaatcta tctcgttcgg
    17341 acgaacaccg taacatgcga ttctcatact ttcttcctcc atttcttatg aaaataaacc
    17401 tatgccatga atcatttgta atacaacgat ccatacaggt gtcagtacca cagcaaaaat
    17461 agttgacagt aaggatgcat tggaggctaa aagtgcctcc ctatcaaaac tgatagcata
    17521 ggctgctgcc actgttgcag ctggtgtagc catcatgata acgattgttg ccagtgcttc
    17581 aaaattgact ggtagaatat tcgttgccgt caaaactgct agaatcacga tattcaatgc
    17641 aggtacaaat accactttga ctagactgta ataccatgaa gttttttcag atgcagcttt
    17701 tttgaagctt acttcaccta atgttgcgcc gattgctagc cacgccaatg gtgaacttaa
    17761 accagctaaa taatccatcg actgaaatag ccacggtgca gtctggtcaa ttcgcaaaaa
    17821 cgctacttgg cttatctctc cttctgcacc tgtaactgac acttgtggca gatacccttg
    17881 gaacagccaa atgaaaaggc cggcaaaagt agcgataatg attggattga cgaacatcgc
    17941 tttgatattt ttagcttcca tcttcaagcc actcattttt atgtatccat aagaatacaa
    18001 aaagattcgg tagccaatat tgaaaattga ggaatacatc acgccgacag gaccataaat
    18061 cgcactgacg ataggcgttc cgaaaaaggt cgtagaacca aagatcgtca ggacacgcaa
    18121 cgttgtttgc ttatcccctt tatagcttaa gaaaaaaggt tttgaaacta gaataagaat
    18181 aatataaata actaatcccc aaatcaagac attgattcct tgcatcagta attctgtatt
    18241 gatatcttgc atgaaagctc gaaatgccag tgctggcaaa gccactgtta agacgacttt
    18301 cgatagaatt tttccaacag attcagaaaa aatccccttt tttctacaaa caaaccctaa
    18361 taatataata aaaactgttg aagtgattgc actgacaatc gacatatctg tcaaagtcga
    18421 tcgaaaaatg tcaaataagt tcatctttct cctcctaatt atgttcattt attcacaata
    18481 aaaaaagctt ttattttgct aatacgtatt aacattttta attttatctt tacattgtta
    18541 cttattgaac aataggaaat taaagaaact tttgaaactt atgtaaagtg att
//
LOCUS       DAZKLA010000048        18427 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000048 DAZKLA010000000
VERSION     DAZKLA010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.048"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            172..516
                     /locus_tag="ACG85N_001428"
     CDS             172..516
                     /locus_tag="ACG85N_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFQ0224204.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="ACG85N_001429"
                     /pseudo
     CDS             517..936
                     /locus_tag="ACG85N_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1028..1378)
                     /locus_tag="ACG85N_001430"
     CDS             complement(1028..1378)
                     /locus_tag="ACG85N_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224205.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="ACG85N_001431"
     CDS             complement(1344..2675)
                     /locus_tag="ACG85N_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFQ0224206.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2931..3497
                     /locus_tag="ACG85N_001432"
     CDS             2931..3497
                     /locus_tag="ACG85N_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0224207.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3642..3729
                     /locus_tag="ACG85N_001433"
     tRNA            3642..3729
                     /locus_tag="ACG85N_001433"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3674..3676,aa:Ser,seq:cga)
     gene            4063..5037
                     /gene="bsh"
                     /locus_tag="ACG85N_001434"
     CDS             4063..5037
                     /gene="bsh"
                     /locus_tag="ACG85N_001434"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0224208.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5362..7257
                     /gene="asnB"
                     /locus_tag="ACG85N_001435"
     CDS             5362..7257
                     /gene="asnB"
                     /locus_tag="ACG85N_001435"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFQ0224209.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7452..7898)
                     /locus_tag="ACG85N_001436"
     CDS             complement(7452..7898)
                     /locus_tag="ACG85N_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0224210.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8126..8275
                     /gene="rpmG"
                     /locus_tag="ACG85N_001437"
     CDS             8126..8275
                     /gene="rpmG"
                     /locus_tag="ACG85N_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0224211.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8363..8881
                     /locus_tag="ACG85N_001438"
     CDS             8363..8881
                     /locus_tag="ACG85N_001438"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0224212.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8958..9914)
                     /locus_tag="ACG85N_001439"
     CDS             complement(8958..9914)
                     /locus_tag="ACG85N_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0224213.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10080..10886
                     /locus_tag="ACG85N_001440"
     CDS             10080..10886
                     /locus_tag="ACG85N_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224214.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10883..11872
                     /locus_tag="ACG85N_001441"
     CDS             10883..11872
                     /locus_tag="ACG85N_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0224215.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11869..12873
                     /locus_tag="ACG85N_001442"
     CDS             11869..12873
                     /locus_tag="ACG85N_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0224216.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13005..13547
                     /locus_tag="ACG85N_001443"
     CDS             13005..13547
                     /locus_tag="ACG85N_001443"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFQ0224217.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13567..14265
                     /locus_tag="ACG85N_001444"
     CDS             13567..14265
                     /locus_tag="ACG85N_001444"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFQ0224218.1"
                     /translation="MNYQQQIKLRVWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14286..14498
                     /locus_tag="ACG85N_001445"
     CDS             14286..14498
                     /locus_tag="ACG85N_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFQ0224219.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14498..15460
                     /locus_tag="ACG85N_001446"
     CDS             14498..15460
                     /locus_tag="ACG85N_001446"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFQ0224220.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15478..15873
                     /locus_tag="ACG85N_001447"
     CDS             15478..15873
                     /locus_tag="ACG85N_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0224221.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16036..16401
                     /locus_tag="ACG85N_001448"
     CDS             16036..16401
                     /locus_tag="ACG85N_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFQ0224222.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16398..18209
                     /locus_tag="ACG85N_001449"
     CDS             16398..18209
                     /locus_tag="ACG85N_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFQ0224223.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18271..>18427)
                     /locus_tag="ACG85N_001450"
     CDS             complement(18271..>18427)
                     /locus_tag="ACG85N_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFQ0224224.1"
                     /translation="VSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKA
                     MRKRVSR"
BASE COUNT         5922 a         3119 c         3810 g         5576 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggtaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat
     2881 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
     2941 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
     3001 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
     3061 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
     3121 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
     3181 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
     3241 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
     3301 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
     3361 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
     3421 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
     3481 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
     3541 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
     3601 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
     3661 aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg
     3721 taccttcctt atatcaaaga ttagaggttg tgaagaagct gtcctgcatc aagtaataag
     3781 aacggccaaa caaaaatagc ttttcctatt ttttcgtttg attgggctta ttaccgcaga
     3841 tgcaagcttt tgaacaccgt ttgttcggaa taaggggctg tgacaagact tttgtcacag
     3901 ccccttattg ttgtactttt aacctacaat attttttgat tccccctgag tactattttt
     3961 tataaatgct agtaaatcaa tcttctttgg cctttttttg ataaaaaaaa tactagatgt
     4021 aagataaaag tgtaaaaacg atttgtttta ggaggggaaa cgatgtgtac cgcaatcact
     4081 tatgcaacga aagaccatta ttttggaagg aattttgatt atgagatgtc ttataatgaa
     4141 gtggtcacta ttactccaag aaattatcgt ttcgatttta gaaaggtaaa aaatttagat
     4201 aaacattatg caatgatagg aatagcagct ggggtatcca actatccact ctattatgag
     4261 gctacaaatg aaaaagggtt gagtatggca ggactgaatt ttcctggcaa tgctgattat
     4321 aaagaattac aagaaggaaa agataacgtt gcaccatttg agtttattcc atggatacta
     4381 gggcaatgct caaccataga cgaagctaaa gaattattgg ctactatcaa tctagtcaat
     4441 atcgatttta gtgaaaaact acccttgtca cctttacact ggttattagc tgataaagaa
     4501 aaatcgatag taattgaaag tatgaaagat ggacttcatt tatatgataa tcctgtcggt
     4561 gtgttgacaa ataatccgcc atttgattat caattattta atctgaacaa ttatcgctcg
     4621 ctatcaaatg gaacaccaga aaatcatttt tcaaaccaga tcagcttgaa tgtttatagt
     4681 cgtgggatgg gaggattggg cttacctgga gatctctctt ctgtttctcg atttgtaaaa
     4741 gccacattta ctaaaatgaa tgctgcatca ggtgattcag aatctgaaag tatcagtcaa
     4801 ttttttcata tactaggctc agtcgagcaa caaaaaggag tatgcgatac tggagaaaga
     4861 aaatacgaat atacgattta ttcttcttgc tgtaacgttg ataaagggat ttattattat
     4921 cgtacgtatg aagacagtca aattactgct gtaaatatga ataaagaaga cttggatcgt
     4981 catgaattgg tcagttatcc agtcataaaa gagcaacaga taaactatgt caattaatta
     5041 atgaatagat ccgtttggta tcatattttc tgatacagtc ataattagac acgatcttta
     5101 ggtcgtgtct ttttttgctg gtttttttgg aaatagcatc cactaatatt tttttaacac
     5161 gaagagaata aggtagcctc aaaaaagaaa acatggtaaa ttagatattg ggactttatt
     5221 ttctgtaaaa acagaaactt tcatcttttg ttttcacgtg acgaaattgt aagagagaaa
     5281 atattcgtcc gctatttatt ctatgataag atgcatatga atgaaataaa cagtaatttt
     5341 atattaggat gtgaaagtta aatgtgcggt attgtaggtt ttgttaacga caaggacaac
     5401 aaaaaaacaa ttattaatac aatgatggat cgaatcgttc accgtggacc taatagttca
     5461 ggagaatata tcgataaaca tgttgcttta ggattcagaa gattaagtat tattgactta
     5521 gagggcggta ctcagcctat ttacaatgaa gatcggacaa agatcattat ttttaatggc
     5581 gaaatctata attaccagcc tttgagagaa gaattgattg cagctggcca cgtttttcaa
     5641 acacatgcag atacagaagt ccttttgcat ggttacgaag aatggggaac agaattattg
     5701 caaaagatcc gcggaatgtt tgcttttgct atttgggata atgaaaagaa tgagttattc
     5761 ggtgcaagag accactttgg gatcaaacca tattattatg cagaaatgaa cggaacattt
     5821 atgtttggtt cagaaatcaa aagttttctg cctcatcctg attttaacaa agaattgaac
     5881 aaagaagcgt tgaaacctta tatgactttc caatattctc cattaaacgg cgaaacattc
     5941 ttcaaagatg tctatcgttt gcctgaaggc cattattata cgtataaaga tggaaaaata
     6001 gacattcagc aatattggga tgcagatttt gaaacaaaag aaacgcattc ccgacaagaa
     6061 tggatcgaaa aaatcgacga aaccgttcaa gcttctattg aagcacatac ggtcagtgat
     6121 gtagaagttg gttctttctt atccagcggg gttgattcca gttatgtaac ctctgtattg
     6181 aaaccagatc attctttctc aatcgggttt gatgataaaa cctataatga agcgattgaa
     6241 gccagaaaac taaccgaact attggactta gataatacag cagcagtgat cgacggagac
     6301 atgtcgttca aagcattccc tttgatccag tatcacttag atgaacctga ttcaaatcct
     6361 tcttgcgtac ctttgtactt tttagcaaat cttgcttcac aaagtgtgcg ggtagtccaa
     6421 tctggagaag gggcggatga actgtttgcg ggataccaaa cgtatggatt ccatacgaat
     6481 tcaaaattca tccgtgtgat tgctcaagga ttgaaaaaat taccaaaagg aacacgctat
     6541 aatttaggac gtaaaatcgg aaaaatgaaa aatttccatg gacggatcca tttatatgaa
     6601 tcacttgctc ctgcaaaaga atattttatc ggtcatgctc gagtgttcga agaatcagaa
     6661 gcatcagaag tgttaacacc aaaatatcaa acagcaccat ctgtggatga aatcatgact
     6721 gttcattacg agaaaacaga aggaatcaaa gatgaagtca ataagatgca atatgtcgat
     6781 cttcatcaat ggatgccaaa agatatcttg ttaaaggcag ataaactttc tatggcaagt
     6841 tctttggaag tccgagtacc tttattggat atcgaagtca tgaaattggc tcaacaaata
     6901 ccaagtaagt ttttattgaa tcaaaacaac acaaaagata ttttcagaca agctgcgaac
     6961 aaacatcttc cggaaaaatg gtcgaatcgt gtgaaattag gattccctgt gccaatcaaa
     7021 gcttggttga aagaagaaca cggctacgaa caagtcaaag cattgttcga agcagatttt
     7081 gcaaaagaat tttttgatca agaaaaaatc atgaagttgc tagatgacca tcacgaagga
     7141 cgtaaagaag agcaaagaaa aatctggaca atcttcagtt tcttgacatg gtataaagtc
     7201 tactttgtag atgaaacgat tccgcaagca gaagcaatcg actatgtgac tgtttgatga
     7261 aaatcaaata tcaataaaaa agaggttttc tcctaccgtt taacactgag tgataagaaa
     7321 caatttcaaa aaacagctct tcccgtttta tagatattat gacttatggt tcggagttgg
     7381 cagaggaaca ccattgtttc gaacataagg gggttgtgac acgattttcg tcacaacccc
     7441 tttacatatg ttcaataatc gatttctttt tcttttgtcc catctttttt ataaatgaac
     7501 aaagttgatt ctttgttttg tgctacttgc tttccttttt cgattgcttc tttttttgta
     7561 tcaaaatggt tgcttgcctt ttttgcccct ttagatttca cgatccattg ttcttcctca
     7621 tattctacga tcacatctga atcaagcagt tttccagcac gaggattcgt atcgtgttca
     7681 tccgtcttgc ttggattttt ttctttttcg aaagttcgtt tttctgattc actagcatct
     7741 tcataccatt tttttgcttg atcaatggca attgggatcg cacggttatc agggtaccct
     7801 tcatctaata atgcatttgc gatgtcgatt gctttttttc ttgtcaattt atctaagttt
     7861 ttcattgatg caggaaagtc cttcatgttc catggcatat tagtatcccc ctttattaaa
     7921 aaacataatc tatataaaca agtgtaaggg tatttataca aaaagaaaaa gattttgttt
     7981 gagaagtaaa accgttgtat ttatcgtcaa gtagttttac ttaacaaaat cgtgaaaaac
     8041 actagcaatc tagaaaaaat catggtaaga taatcaagtc tgagagtcat cttagaggat
     8101 tcttgtaaaa atgggaggga aaaacatgcg cgtaaacatc actttagagt gtacttcttg
     8161 taaagaacgt aactatctaa caagcaaaaa caaacgtaac aatcctgatc gtttggaaaa
     8221 acaaaaatat tgcccacgcg aacgcaaagt tactttgcac cgtgaaacaa aataatgttt
     8281 ttaaagttct caattccttg ctggtcaaag aattgagact ttttttatct caaaacatct
     8341 aaaatcataa ggagcggata atatgtttat ttccatgtgg gcacaagata aaaatggtct
     8401 gatcggcaaa aatggtttgt taccatggcg attgccaaat gatatgcgtt tctttcgcga
     8461 acatacaatg gatcgaattt tagtgatggg aagaaagacc tatgaaggaa tgggggatct
     8521 ttcacttcct tatcgccata tcatcgtgtt gacaacgcag ccaaattttc gcacgaaaga
     8581 aaatgcggaa gtaatgcatt cgatcgatga acttttgtcg tatagtcaaa cgatctcaga
     8641 agatatctat gtttcgggtg gtagtcggat ttttcaagaa cttcttcctt acactggtcg
     8701 gatctggcga acattgatcg atagcacgtt tgaaggcgat acgtatattg ggaatattga
     8761 tttttcagac ttcgccctta tagaggagta tgaggggata acggatcaag aaaatctata
     8821 tgcccatcgt tttcaaaaat gggaaagagt aaaaaatagg gttgcttcaa aagaagaata
     8881 aaatctatgc gtctagtttg ttaataagaa aaaaagcata tcacgactaa tcgtgaagat
     8941 gcttttttta gttaaaatca atttagtttt tctttgatat cattgaccat ttcctgactg
     9001 gcaataggtc cgttatatag ccaactagat tcaggtccga attgcagtac atgattgtct
     9061 gtcactgctt taagattctt ccagcttggt tcattgaaca tggcagcatc tttgtcactg
     9121 ttcacaagga tgatgtagtc agcatctaat tcagctagct tttcagagga aacagcagac
     9181 cagtcagaag tggctttttt ggagacctct tccactagat caggaacacc aaattccaga
     9241 tcgtcgtata cgattcttcc acttgaccga tttttgctga ccataaaggc tgagttgttg
     9301 gtcacccaca acactgcagc tgtcttgttc tcgattttat ctgctagttc ttctcgagtt
     9361 ttcttgatgg atttttgata atcggaaatg atcttttctg cctctttttc ttttgccaaa
     9421 gctttgccga catcttttag ttgttcttcc catgtgacat ctgttccgtt ttttacaaca
     9481 taagttggcg cgattttgct atattgttcg tatttcccgc cttcgacagt agcggaagaa
     9541 gaaatcaaca gcaaatcagg ctcaaagctt aacactttct cataaggaag gtcataagaa
     9601 atcgttggga catcctttaa atcatcttgc agataatctt gaatggaacc actgccaaca
     9661 gtccactggg cgataggttt ttcccctagt gcaatcagat agtcttctaa gtaactgccg
     9721 atgatccgtt tgggcgcgtt tggaatctct actttatgtc caagtgtatc tgtgactgta
     9781 tgagtactat tggatgaagt cgtcgaattg gcagttgtag ctgtttcttt atctgaagag
     9841 ggggagcaag cgctcagtac tccagccagc agcaatatgc tagataatgt gaatactttt
     9901 gtttttaatt tcatttcttt cctcctaaca taattgagaa tctttctcat tggttattat
     9961 tcctgatatt tcgaacgctg tcaaccgctt catttggcta tagatatgaa agtagcgctt
    10021 gacgaaatgt tgaaacagcg tcataattga gaatgatttt cagcgaaagg atgggtgaaa
    10081 tgacgagatt aagagcagct gacattgcag ccggctacga aaacaaaaaa atcattgaaa
    10141 acatgtctgt tacgattcct gaagggaaaa tcactagttt gattgggcca aatggaagtg
    10201 gaaaatctac tttattgaaa agttttaccc gtatactacc tgtgaaatca ggaaagatca
    10261 ttgtcaataa ctctgatatc tctacataca gttcaaaaga acttgcaaaa aaaattgcat
    10321 tattagcaca aactagtgaa caccctacag gtatgacagt agaagaagta gtatcttacg
    10381 gacgataccc ttatcaaaaa ctgttcagtg gcatggacac gcaagatcat gaagcaatcg
    10441 agtgggcgtt ggatgctaca ggtctagctg gatttcgtat gcgggatatc gcaacgctgt
    10501 ctggtggaca aacccagcgt gtctggatcg ctatggcgct tgctcaggaa acagatatct
    10561 tgatcttgga tgaaccgaca acatttttag atccggcaca ccaattagag atcctacagt
    10621 tattgactga aatcaatcaa atgaaacaaa caacgatctt gatgtcgatt catgacatta
    10681 atcatgcttc acgtttttca gattatttat tagcaatgaa aaacggtaag ttgatcacag
    10741 aaggtacacc tgaagaagtg atcacaaaag agtgtatgaa aaaaatattt gagattgatg
    10801 caacgattgt cacgcttcca agaacaaata aaccagtttt tctaacctat gatttgttgc
    10861 caaaagagca ggaggatcaa ttatgaggac tatgaagaaa aatagactcc tgcttttggt
    10921 ctgttttgtt cttttgatta ttttttttgt tgctctttca aatggcgcag cgactgtggg
    10981 aaaagcagat ttttggcagg cgttgtttga ttttgataga tcaaaacaaa gtcaccaaat
    11041 catacgaaat attcgtttgc ctcgtgtggc gggagcattt cttgtaggga gttgttttgc
    11101 attgagcggt gcattgatgc aaggagtgac aagaaatcct ttagcagatt caggcttact
    11161 aggaatcaac gcgggtgctt cgttggcaat ggcccttagt tttgcttttt ggcctaaagc
    11221 ttctgatttt tctgtgtttc taatgtcatt ggcaggttct tttgctgtaa ctttgctcgt
    11281 atttggtttt ttgcagtttt cgaaaatggg catgaatcct ttacagctga ttttagctgg
    11341 cgtagctatt agttcttttt ttactgctat cagccaagca cttacccaat tctttaatct
    11401 gcagcaggat cttgcttttt ggtttgtagg cggagctgcc aatatcacat ggaaacaatt
    11461 agcagtattg tttcctttat atatacttgc tgttttggtt tctttttcat taggaaaagc
    11521 aatatctttg attcatttga gtgaaacaca tgcattagct cttggaagtc atcctaaacg
    11581 aacgcggttg attgttttct gtctagttgc agtgttagca gccattgccg tttcgttagt
    11641 cggacctgtt tcatttattg ggctgttggt tccgccagtt gttcgcggaa tggttggaac
    11701 ggactatcgt tatgttctac ctgtctcttt tttagttggc ggtggattgg ttatgttggc
    11761 ggattttaca gcgaggatga tcaatccccc ttttgaaaca ccttttggat tagtcattgc
    11821 attgattggc gttccgtttt tactgataca agtaaggagg gaacaacggt gaaaaaaata
    11881 tattggacgt tctatttctt gctttttgta cttgtcatcc tgtctatagg tagtttaatg
    11941 attggtacgc cttttattta cctggatcag ctttttgcca cgttcatagg gaaagaatca
    12001 tcttctttgg cgctgattgt tagagaattt cgagttcctc gattactgat cagtttatta
    12061 gcaggagctt gtcttggcgt ttctggttat atcttgcaag gagtaacaag aaatgaattg
    12121 gcagattcta gtatattggg cattaatgcc ggagcaggat ttttagtgat gctttattta
    12181 ggtttctttt cgcactattc gctgccgttt ctattgctag ttgtagcctt tctaggtggt
    12241 gttttagctg cttgctgtgt ctatcttgcg gcctatgatc gaaatggctt tttgggtatg
    12301 aatcgaatct tattgtccgg tattgccgtt aatgccggcc tttctgcatt aacattgcta
    12361 tgcacgatcc aattatctaa ggaaaattat ggatttgtga atgcatggtt agcgggatca
    12421 atctggggtg cgagctggcc ttatgtttgg gcattgcttc catgggcggt tatcttaatt
    12481 ccgctaggag gactttatgc acagaggatt gtattgctat catttggtca agaacgcgcg
    12541 caaggattag gccttaacgt ggctaaaagt caaaaaatcc ttttgttgtt agctgttgct
    12601 ttggcaagcg gtagtgtagc ggtgacaggt agtttatctt ttgtaggact actagctcct
    12661 catgctgcga agctagtcgt acgtaaagaa aataactgga cctttgttct gagcggtttg
    12721 ttcggttcca tttttctgct tagtgcagat atggttgcta gagtgattct actaagtggg
    12781 gaagtaccta caggaatttt gattgccctt tttggtgcgc catattttct gtatgcactt
    12841 tttatcagac aaaaacacgc gctgaacagt tgaaattcat taaaaaagga aggccaatca
    12901 tttctaggtc ttctttttat tctatttatg catttatttg aaagttcgat tgctattttt
    12961 ctttttttcc tataccattt tatgagagaa ggaagggtga gtttttggac caattgaaag
    13021 taagattacg ccggaaagga atcgaaaagc tgaaaaatct ggccaaaagt ggcaagaaaa
    13081 aggaaaaaga acagcgaatc tataaacagc tattcgccag cgagtactgg aagtcagcaa
    13141 aagtgatagg gacgacttta tcgaatgaat ttgaactgga tacacagccg gttatccaac
    13201 gagcagaaaa agaacaaaag aaaatagttg tacctaaaac tttgccgaaa cgtcagatgg
    13261 cgttttatga aatggatgaa cagtccgttc ttgtacgttc gagttttggt gttcttgagc
    13321 cacaatcaga gaagctctat ttgccggaga tgatcgatct cctcattgta ccaggtatta
    13381 tctacaatag agccggttat cgaatcggtt ttggcggtgg ttattacgac cggtatttaa
    13441 ataattttca taaaaaaaca tgtagccttg tttttcgtga acaattgaat gaacaatggc
    13501 aaccggagag ctttgatcag aagatccaac aattgtttat tgactaaaat gaaatgggag
    13561 gttttcatga attatcaaca gcaaataaaa ttgcgtgtat ggttaagacg accgttatgg
    13621 acctatgctt ttttagggat acagacagcg gtcttcctca tgatggaatt atttccgcga
    13681 cttgagatcc cttattatgc cggaatgtat ggtccttatc ttgttcactt caatgaatgg
    13741 tggcgtttag tgacaccaat cttcatccat tttggactga tgcatttcgt aatgaactca
    13801 ctgatactgt attttatggg gcagcaaatc gaggcaatct atggccattg gcgttttttc
    13861 ttgatttata tgttcagcgg gataatggga aatacagcaa gctttgcttt taacgaagcg
    13921 aatgttctgt ctggtggtgc aagtacttcg atattcggtt tgttcggtgc gttgtttatc
    13981 ttagggtttc attttaaaca taacccagcg atccagcagc tagtcagaca ttttctttta
    14041 tttattgtgt tgacatttgt ttttggtctt ctggatacct ctgtagatgt ttggggacat
    14101 gtcggtgggc taattggcgg attagtgtta gggaatgttt tgggtctacc gaaacaacag
    14161 acaagctatt cgatacacca aaggattctt tctacactag tttttgtatt tctttttgta
    14221 atatgtattt tactgggttt aaaaaaatat ggtttacttg tataatatga aggaagtgct
    14281 taaagatgga gagcttatat gatgtacagc agctgttcaa aaaatttggc atctttatct
    14341 atgtgggtgc acgcatctat gacattgaat taatgactat cgaattgcgt aatctgtatg
    14401 atagccgttt gattgatcat gatacataca tgacagcgtg gcgcatatta aaaagagagc
    14461 accgaattga agaaagccga aagaaaggaa atttgtaatg gacaagaaga ttattgggat
    14521 tgatttaggt ggaacaacag caaaatttgc aattttaaca ccagaaggag agattcagca
    14581 aaaatggagc atcgatacca atattttgga tgatggtaag catatcgttc ctgaaatcat
    14641 tgaatcaatc aatcatcgtt tgaatttata taatatgaaa gccgaagact ttatcggaat
    14701 cggcatgggt accccaggaa gcgtggacag cgaagctgga acggttattg gtgcgtataa
    14761 cttaaactgg acagaagttc aatttgtgaa aaaactgatc gaggcaggaa caggaatcaa
    14821 attcgctatc gataatgacg cgaatgttgc tgctttaggt gaacgatgga aaggtgctgg
    14881 agaaaacgat ccagacgttg tattcgttac tctaggaaca ggtgttggcg gaggtatcgt
    14941 tgctggcgga aatttgattc acggcgtgag cggagctgga ggagaaatcg gacatatcac
    15001 tgtggatcct gaagggttcg aatgtacttg cggtaaacgc ggatgtttag aaactgtatc
    15061 tagcgctaca ggtgtggtac gcctaggtcg tttcttggca gaagaatacg caggggattc
    15121 aaaactgaaa gcaatgctag acaacggaga agaagtaaca agtaaagata tctttgaaat
    15181 ggctcaagaa gacgatccgt ttgcattgat ggttgttgat cgcgtatgct tctacttagg
    15241 tttagcttgc ggaaacttgg ggaatacatt aaatccgtca agtatcgtgt taggtggagg
    15301 agtatcggca gcaggagaat ttttacgtag ccgtgtagag aaatatttta aagaatttac
    15361 tttcccgcaa gtaagagaat caacaaaaat caaattagca gagcttggta atgaagctgg
    15421 tgtcattggt gctgcctcat tagccttgaa atttgcttaa aaaaacggag gcattacatg
    15481 gttttatggg taattaatat cattttgcta ttgattgtgc ttgcaatcat cctttgggaa
    15541 gtctatctac gagtaatggc caaacgttca gcaacgactt tgacagaaga agaatttcga
    15601 gaaggaatga gaaaagcaca agtaatcgat gttcgcgaaa aagatgtgtt tgatgctggg
    15661 catattttag gtgcaagaaa catcccttac actgttttga aagattcatt aggatctatt
    15721 cgaaaagacc agcctgtcta tatttacgat caaaagaaaa gtttgagcat ccgtacagca
    15781 aataaattac gtaaagcagg ttatcaagag atttatttac ttaagggtgg atatgacgga
    15841 tggtctggaa aaatcaaaag taaaaaagta taaaacagat gattgtatga cagctttaaa
    15901 aaccgaatca atcattagat tttgaccaag atcacatgaa cggcgcaatg gatagttgtg
    15961 ccgttcattt tttttatttt gtgctattat ctacatatat aagctaagcg aaaaggtaga
    16021 aagaaggaaa caactatgat caaattaaaa cgtgtttacc ttgatgctga aaaagaggat
    16081 ggctatagaa tactcgtgga tagattctgg ccacgaggaa tttcaaaaga aaaagcgcag
    16141 ttagatctgt ggttgaaaga aattgcacct agtaatgaag cacgcaaaac ttttgaccat
    16201 gtaccagaac gatttgcttc atttgcaaag aaataccagc aagaattgat tttcgaagaa
    16261 gaaaagaagc aagctatttt taaactcagt caacttatga aagaacatcc agtcattact
    16321 tttgtttatg gtgcaaaaaa tgaacatcag aaccatgctg ttttattgaa aaaatttatc
    16381 gaaatggagg catgcaaatg aaaataggga tcattggtgc cggacctaga ggattaagtg
    16441 tagcagaaag gattctccat aataatcagg acagacattc tcttgaactt tttcttttcg
    16501 atcccaatgg tcctggaggt cgagtttggc ggttgaatca gccaccggag ttattaatga
    16561 attctgtttc tcaacaagtg acgcttttta cagatgaaac attgtccagt ggaggattgg
    16621 taacacaggg acccaacttg tttcaatgga gtaaaggacc tgctacagat tatatcgaac
    16681 agacggtaac caaaaataag caactaatta tagaagaaat caatcgtctg gaaaaagatc
    16741 agcaatctac tcgatgttta tacggactct atcaacgctg gtttttctca aagttgcaag
    16801 aagaatttcc agcttctatt cagtttgttt attctttagt aaaaaaagta agcaaaagaa
    16861 aagatggtta tcttttacag acagatcagc aaagctattc tatcgatcag cttgttttag
    16921 caagcggtca ctgggaaaat gatgagacag aagaagaaag agtgtgggta gactacgcaa
    16981 agaaaaatgt tttactgtat cagcttcctt ctaatcctgc agatgtagat ctttcgaaga
    17041 taccgcctaa agaaaatgtc tttttaagag gtctaggctt aagttttttt gattatgtcg
    17101 gattgtttac agtatcaaga ggagggcgct tcgagaacga aaagggaaaa ctggtctatc
    17161 atccatcagg caaggaaccg attgtataca gtggatcaag aagggggctc ccttattacc
    17221 ctcgaggaag aaatcaaaag caaggcggag caatggcatg gccacggtta ctaacaaatg
    17281 aaaacctgga gcaatggcat cagtcgggat ttttgactgg cgaaacattt tttgactatc
    17341 tgaaaaaaga cgttgagttg ttttattaca agaaaatcat cgaagaatat cacttgccga
    17401 ttccaaagaa aaattttgag catgattttc tctccctctc ttcagaggag tgtctcggaa
    17461 agcatccagc actaatccct tatagatggg attggtcgta tttagaaact cctcttgcct
    17521 atcagaatga gcccttttca gaagctagcc gaatatttat tgaagggcag ataaaagaag
    17581 cggaaaaagg caattgcaca ggagcatttg cttcaacatt tgatgcatta aaagactggc
    17641 gtgatccaat ccggcaggct attgaatggc acgttttttc tgctaaggaa tacaaagaaa
    17701 tactatgggg atggtttact catctaaata gctttttaac gatcggtccg ccaatcattc
    17761 ggacgaaaga attagcagca ctgatagatg cgggtatttt tcatttgatc gaaccgccat
    17821 ttgatattca gcaaaagcac aaccgctatg ttatttctct acaagataaa gaaatcagca
    17881 gtcgctattt gattgaagct cgattgccac aaacaagctt ttctcgttca agtaatcctg
    17941 tcataaaaaa tttgaaggaa aacaaacttg ttcgcccttt ttcattttca gataatacag
    18001 gaagttatca ttcagaagct atagatgttg agcgaggtac gaatcgcgtg attgatgctt
    18061 caggggaaat ccagtctgac ttttattgct atggaatacc tgttgaaggt gtggattggc
    18121 taactgcagc agtagctaga ccttatacag atccatggaa tctgagacag gcagataaaa
    18181 tagcacaact tatattgact gacaaataaa aatacgcatg atgacttatc tgttttttag
    18241 aaagtcttca tgcgtatttt taaataatta ttaacgagaa acgcgtttac gcattgcttt
    18301 tttacggttt tcttcgatca ttgcttcttt ttcgtcaatt ggatcgatca ctgttttttt
    18361 cacagaagca accaaaccag caacggcagc ggctgttgcc agacttccta ctaatattcc
    18421 agataca
//
LOCUS       DAZKLA010000049        18219 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000049 DAZKLA010000000
VERSION     DAZKLA010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.049"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..2198
                     /locus_tag="ACG85N_001451"
     CDS             138..2198
                     /locus_tag="ACG85N_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFQ0224225.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2281..2850
                     /locus_tag="ACG85N_001452"
     CDS             2281..2850
                     /locus_tag="ACG85N_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0224226.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3025..3831)
                     /locus_tag="ACG85N_001453"
     CDS             complement(3025..3831)
                     /locus_tag="ACG85N_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224227.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3969..5483
                     /locus_tag="ACG85N_001454"
     CDS             3969..5483
                     /locus_tag="ACG85N_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFQ0224228.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5886..6869)
                     /locus_tag="ACG85N_001455"
     CDS             complement(5886..6869)
                     /locus_tag="ACG85N_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFQ0224229.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6874..7521)
                     /locus_tag="ACG85N_001456"
     CDS             complement(6874..7521)
                     /locus_tag="ACG85N_001456"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0224230.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7606..8238)
                     /locus_tag="ACG85N_001457"
     CDS             complement(7606..8238)
                     /locus_tag="ACG85N_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0224231.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8254..8805)
                     /gene="coaC"
                     /locus_tag="ACG85N_001458"
     CDS             complement(8254..8805)
                     /gene="coaC"
                     /locus_tag="ACG85N_001458"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFQ0224232.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8802..9539)
                     /locus_tag="ACG85N_001459"
     CDS             complement(8802..9539)
                     /locus_tag="ACG85N_001459"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFQ0224233.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9536..10525)
                     /locus_tag="ACG85N_001460"
     CDS             complement(9536..10525)
                     /locus_tag="ACG85N_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0224234.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10783..11325
                     /locus_tag="ACG85N_001461"
     CDS             10783..11325
                     /locus_tag="ACG85N_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFQ0224235.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11342..12427
                     /locus_tag="ACG85N_001462"
     CDS             11342..12427
                     /locus_tag="ACG85N_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224236.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12439..13245
                     /locus_tag="ACG85N_001463"
     CDS             12439..13245
                     /locus_tag="ACG85N_001463"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0224237.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13246..14073
                     /locus_tag="ACG85N_001464"
     CDS             13246..14073
                     /locus_tag="ACG85N_001464"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0224238.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14070..15143
                     /locus_tag="ACG85N_001465"
     CDS             14070..15143
                     /locus_tag="ACG85N_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0224239.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15571..>18219
                     /locus_tag="ACG85N_001466"
     CDS             15571..>18219
                     /locus_tag="ACG85N_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0224240.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAY"
BASE COUNT         5885 a         3225 c         3719 g         5390 t
ORIGIN      
        1 ttttcttgaa acaataaaaa atcaaaaaaa atactctcct aatagtccat cttcttttga
       61 ttttatttat ataatgaaat catcttatga aaattactac ttcatgttta gacaagagta
      121 gggaggacta agaataaatg tgttcaaaaa gacgttcaaa acaactaaat aaacacgctt
      181 gtcgtttaga taaggaacgg tcgtgttctg aaaaacacta cgttaagttt atgatgcgca
      241 cgatgctgat cagtttatct gtaggtgtct atatgcatgt acccgttcag gcaacgacac
      301 aaaccatcaa ggtcgtacgt acctctgttt ctgagaccac tgattcccct atcactatta
      361 ctcatacttt cactgatgca aatggaaaac agctacttcc tgcattgact tcctataata
      421 aagtcagtta tcctccaacg ataaaccact actggttaat tcctaacaaa acattatgga
      481 catcggaaac cggaagcagt gtgccgcttt ctatcttttc cgatatgact gatcctacaa
      541 aaggtgatca gttaaaacag atcactgata ttttgaatca tgctttgaca gatataaagg
      601 acggtggaag cgcacaagtt tcttatgtat atgaaaaaga ttattcacag tttgaaagaa
      661 agaaaatcga tgttctggca gggacaaaag tttcgatatc tgatctggta gaaaggctga
      721 agaacgcaga tggaacacaa ggagatatgt ccaaagtaat ttcaaacaat gggaatatcg
      781 tggatacgac taatacagga acatctacta tttccttgtc ctattttgat ccaattgctt
      841 taaaaacaat gactgcatct gctgtgatcc atgttcgtgc tgatcaaaca gctatcaaag
      901 gaaaagatct tcacttaaga acaggagaga gttacgcccc taaagatctt gttgaatatg
      961 taacaaaaag cgatggaact tcaggaaata aagaagaaat caaaatgaac gggacagtag
     1021 atactacaac tccgggagaa tatcccgttg tattaactta tatagactcg aaaacatcaa
     1081 atgtggcaga aacaacagct gtagtttatg tagaagcatc tgctgcttca tcgactgtca
     1141 tcactcattt tatagatagt caaacaaata aagaacttca agcagaaaca catatgagta
     1201 gtgtaaatcg tattcctgat ttgcgcctac ctgctgaatt ttctgatcga gtgccaaata
     1261 tagatttatc gacttatgaa caaaatggga aagtcacttc tatttctgat tggatgcaag
     1321 caaacggatt agacagaaaa agtcactggt cggagatcct tgaagtactt cactcagaac
     1381 tgatgaataa taggaagcaa tcaggagggg aaattacctt tacttatgtt tatataccag
     1441 acaatcgttc attagaaggt catgatctta ttgttcatcc aaacgaaaaa ataaacaaag
     1501 aagagttgat ccaatcagca acaaattcct taggtaaacc cgtaccgtta aaggatgtat
     1561 cggttacaat caatgaagta ccattggagg atggttattt gaccaaacaa acaggtgatt
     1621 ttacgattat gtatagttat catgacccgt attcactcca agaactccat gctcagtcac
     1681 tattacatgt tttaccatta aacaaacaga tagatcaaaa agaacaaaaa agacaagaag
     1741 aaagcttgtc agaaaaaaaa caacaaatgg ctcatgcagt ttctccaaca gggaaagtaa
     1801 aacaaacaag aagcctagag ttagcaagaa tatctagaaa aatctcagaa tctaataaaa
     1861 cgataaggca agaagctcat ttggccccac aagtttctgg attggaaaat gaaattttag
     1921 agcctaaaat aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg gaagatcaga
     1981 aggaatcaga tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt gattccaccg
     2041 atgagcatct gcagccgcaa gtaccacttc ctagtccggg aacacccgca cctggtggag
     2101 gtattccttc ttctgcttta ggagatttca tgcgaggtat ggcaggaaca tgggtatacg
     2161 ctaatcgaaa tgaatttgac gcagatgact ggttctgatt tatatctcta tctgttatag
     2221 tcaaataagt agctgagtca tatgttattt ggcataatgg agacgaggag ggatagaaaa
     2281 atgttagata aaacgttacc ttacgcagaa atctggatga caagaccact tcaacaaaat
     2341 gttccaatca cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa agaagatgaa
     2401 tctgcttggg cggagattga gacttccgtt ggggaatttc aaacgacaga tgaagcactt
     2461 gagtattttc aacaaaactt tgctccttat cctgaagaac ttgaaagaag gatgtttttt
     2521 gtagagacaa aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa aaaatcaaga
     2581 gagcaagtgt atccggtttt tcattggtta gcagtgaaac cagattacca aggaaaaggg
     2641 ctcgcaaaag cattgactgc cagagtcttg caagaatttc ctaccctcca tacggacgga
     2701 ccaatctatc ttcacacaca gacatggagc catcaggcga ttgctcttta taaaaaaatg
     2761 ggttttactt ttattgctga aaatctagac ggcacaccta atccagatta tgaaaaagtt
     2821 atgcaggttt taagtaaatt agataaataa cgaaatggtg aaataagtcc tcagtattgg
     2881 gataaaaaac gaaatagtca ttcctcatat cttacttttt tgagttaata ctatcggata
     2941 actaatggca tgccatacat tcaaaaaaga aggggcagtg acaaaagtat tgtcactgcc
     3001 ccttctcttg ttttttagca tgttttatcg tttgattttt ttaggaataa aattcttgat
     3061 gaacgtaaat ttttggtaat cgtttcgact atagaggtaa ccaaatacac tgaaaacaac
     3121 tgctccaata aaattaacaa ataaatcctt catagtatcc atgattccca cgtctaagta
     3181 accattttgg atgattgttt tatctgtttt accatctgca ttttgacttt caatgattgt
     3241 ccgagtgata tctggaacat gatagacagt gtttccgtct ttacttaact ccacactatt
     3301 gatttgctgg atgatcgtat ctttttgcat atcgaattgc agtaatttat ctgctgaaaa
     3361 ttccacaaac tcccacataa caccaactgt catcgaaaaa cagaaggaaa caactgctag
     3421 taataaagga gtcaagttca tggtttttgt atttttattg agcaaataaa caagggaaaa
     3481 gccgacaccc ccagctaaaa atccgctgaa tgcatgtaga aaagtatccc acattgaaaa
     3541 atagccataa aaatcactta actctcctaa aactgttgaa ctaaaaataa aagataacaa
     3601 aatgatttct agcatatttg gaatctgtat ctttaatatc ttttccagaa aataagggat
     3661 ttcaaataaa attaatgtca gtaataaaag cattgtattt ccccagtgtt ggttccagac
     3721 ctgatccact agcataataa cgacgatgat acgtaataaa atatagattc cgtttgtgat
     3781 tctacctgca gtttttgtac tgttcgtaaa aaaggttttc aaatgattca taaaataact
     3841 ccttcatgta tatccttaat tatactaaga agcgtgtgtt taccaaagct atatgactta
     3901 aagaaaaaca gagttgatat tgtgaaaaaa atcacttaat atagtataag gagaaaaggg
     3961 ggatacaaat gaaaaaatta gttgctggtt tattaacagc cgttgcactt cttgctatga
     4021 gcgcatgcgg cgcagatcag aaaaatacag cgcagacaag ttctagtacg aaggaatctg
     4081 ccgaagtatc atcaggcgct tctgaacaag tatatacaga tccaaaagaa atgaaagaat
     4141 cttatgatgt cgtgattatt ggagctggtg gtgcagggat ggcagcagct atcgaagcaa
     4201 aagatgctgg gttgaatcca gtaattttgg aaaagatgcc tgttgccgga ggaaacacat
     4261 ctaaatcatc aagcggtatg aatgcttcag aaacaaaatt tcaaaaagaa gaaggaatca
     4321 aagactcgaa tgatgccttc tatgaagaaa cgctaaaagg tggtggagga acgaatgata
     4381 aagaaatgct tcgatacttt gtcgatcact ctgctgatgc ggtcgattgg ctagatcaaa
     4441 acggtatcaa actggacaac ttaacgatca ctggagggat gagtgaaaag aggacacatc
     4501 gtccgtctga tggctcggct attggtggat acttagtgga ggggttattg aataacgtcc
     4561 atgagcggga aatccctatt tttgtcaacg cagatgtgac agacattaag aaaaatgggg
     4621 aaacggtcaa tgaagtgact gtacatgttc aaggagaaga accaaaagag gtcaccggaa
     4681 aagcaatcgt agttaccaca ggcggttttg gtgccagcaa agaatttatt gaaaaatatc
     4741 gtcccgattt agctgattat attacgacaa atcaagaagg atcaactgga gatggtatca
     4801 ccatgattga aaaattaggt ggacaaaccg ttgatatgga taaaatacaa attcatccaa
     4861 ctgtgcagca ggatgagggg gtcttgattg gtgaggttgt tcgcggagaa ggagcaatct
     4921 tggtcgatca agaggggaaa agatttgtca atgaaatgga tacaagggat aaagtatctg
     4981 cagcaattac agcattgcct gaaaaatcag cttatttgat ttttgaccaa ggcgtgcgtg
     5041 atcgtgccac agctattgaa ttttatgacc aaaaaggata tgtcacaact ggaaagacaa
     5101 tggaagaatt agcagaaaaa atcgatttac caaaagaaac actagcaaaa acagttgatg
     5161 gatggaatac ggatgttgcg gagaaaaaag atgctcaatt caaccgtgcc acagccatgg
     5221 aacatgactt atcgtctccg aattattatg cgatcaaaat cgcaccagga atccattaca
     5281 cgatgggcgg tgtgaaaatc aatacgaaca ctgaagtatt agataaagaa aaccagccaa
     5341 ttagggggtt atatgctgct ggtgaagtca caggtggctt acatggaaac aaccggatcg
     5401 gtggaaattc agttgcggag atcattattt ttgggcgaca agcgggcata caagcagctc
     5461 gatttgcttc ggaaaatgaa tgaaatagaa cgtaaataaa aagaggttgt gaaatcagcg
     5521 ttttgagcta ataccgaggg tcagctgatt gaacactgtt tagtgaagag gttgtgaaaa
     5581 agctgccctg catcaagtaa taagaacagc caagtaaaaa cagcttctta tgtttttaac
     5641 ttggttggac ttattaccgc agatgcaagc ttttgaacac cgtttattcg gaaaaagagc
     5701 gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag
     5761 cagaagaaat tccttcggaa ataagctgaa attcacaaaa atttgaaaag caattttcgt
     5821 gaatttcttc ttatttcttg gagttaaacg cttctgtccc agcctctttt ttgatcagca
     5881 tgattttatt cagttatctt caaaatgatt tttccgatgt ttttgtttgc ttccatatat
     5941 tcatgcgctt gtttgactgc gctgaattgg aatgtacggt caatgattgg ccgaatcttg
     6001 ccattttcta gaaaaggccc agctttttgc ataaaagttt gtgcgagctc tgctttatac
     6061 tgatcacttc taggtgtcaa taatgtacca agcagttgga cacgtttccc aatcaataca
     6121 ccaaggtcta tctcaggcac gatggtccca cctagcatac caatcaagac ccaacgtcca
     6181 tcaacggcta tagaagctaa attcttttcc cagtaagaag cgccgatgaa atccaaaata
     6241 acatcgactc cccgaccttc agtgattttt cgtacctctt cttgaaagtc ttgtttttta
     6301 tagttgatca attcatctgc accaagttct ttacaaaagg cgagtttttc ttctgatcct
     6361 gcagtaacga tgattttcgc gtgagaaagc tgtctagcta attggatcgc tgctgttccc
     6421 acaccacttg ctcctgcatg gatcaagaca gtttcgcctt cttgtaattt tccaagccag
     6481 taaagtgttt gataagcagt caaaaaaact tctgagattc cagcagcaga aacatagtct
     6541 agcgtatcag ggagaaggat tgcacgatcg gcaggcatca cagcatattc ggcataacca
     6601 cctagattga ctaatccgta tacttttgtc ccaggagtaa aagtggagat atcactttta
     6661 ttctcaacta cctctccagc tacttcgact cccaaaatag ggtagggagg tttcaaagag
     6721 actgattctc ttttcataat atctgttcga ttgacagctg ctgtatggac tttgatcagt
     6781 aattcgcctt gttttggttt aggaacaggc acttcaattt gttctaatac atctacagaa
     6841 ccaggatgct tgattgaaat ggcacgcatg gatctattcc tcctttgccc attcttggaa
     6901 tgctgtattc aactctttgc taccttcaga aagtactaga gtcgtacgag aaatatccgt
     6961 ttcttccact agagcggcaa gtacagcagc aactgtagag cgagtgatcg tttttgatgc
     7021 cttctcatta ggcttagtta attgaatgga agtaacttct tcatcgttag tcaatgtgac
     7081 tggttgaaga atgacaaaat ctaagttagt tcgtttcagc cattcatctg cataatgttt
     7141 agtaatataa taatctgtca tggactcagg ccattttgct ggttcatctg cataaacagc
     7201 actgaccatc agatagcgag agacatgcgc attttcagct gcgatcactg ttttgattgc
     7261 gccatccaag tctacttgca gaagattctt tccttgagag ccggcagcaa aaatcaatac
     7321 atcgatgcct ttgaatgctt cggtcatttt ttccacggac caagttaaat caaaagagac
     7381 atacgaaaca ttctcttttt ttactaatgt ttgggaagca acatctctca cccctgcaaa
     7441 aatttcatgg gaacttggag ctaaatcttt tatcaggtgt cggccaattt gtccgtttgc
     7501 acctacaaca aaaattttca tttggatcat cctttcgacg tcaaatcttt ccattcaata
     7561 gtaacaccaa aagagaaaaa aatcagagat cggctacgat gactatcagt tagtaagaga
     7621 agggtgcttg aagtatctta acaagacatt gcatactgct gcagtcgcaa gacctgcgac
     7681 gattccttca ggtataccac ttgtaaaaat aaccgctaga atagctgaat aaacagcatt
     7741 taaatttgtt ccgatgactt tggcataatc ttgctggaat aaaaagtaga tcatattcat
     7801 cactaaaatc gtattggtca atgaaccgga aagtccagct aagaataaag aaacggaatc
     7861 tgctttttgt ttcattagtt tgcgcagacc tttataaacg aaataaggaa ccaccccaat
     7921 caagatgcga ggaataaacg cgatcagcaa ggctttccag cttccatgtt ctgttccgat
     7981 gactgggata aacggagaaa agacaaaaga caaaggtgtt tggataactg tgctacggat
     8041 catgctgatc agtccaaatg taccgccaag aaaagcacct agtcttggtc ctaaaacgat
     8101 cgaagcaatg attacaggaa tatgcatggt tgttgcattg attggaccga tagggataaa
     8161 acctaaaggc gtaacagcta ataaaatcaa aatagctaaa aacatcgcag ttaatgtaaa
     8221 gttttttgta tttttcataa gtactccttt tttctaagca tttctttcat taagagtcag
     8281 catgacctgt tcgatgatcg tctgaatatc agctaaggca ccttttccaa aatctccaca
     8341 agctaaaaga gattctcttg gttcaatttc ctgatagccg acagatctca atgtttccag
     8401 attacgctga gtgatcgggt tttgatacat attcgtgttc atggcagggg caatcaattt
     8461 tggtgttccc actggaagcg ccattgcgac tgttgaaaga agatcgtctg caatgccgtt
     8521 tgctagcttg ccgataatgt ttgctgtagc aggcgcgatg agaaacaagt cagcttgttt
     8581 tgctagttcg atatgattga ttacctgcgg atttgattct tgcatgacgt ctgtatggac
     8641 tgcgcgcttc gacagagatt gcaaggttaa tggcgtgata aaatgtgtgc tgcttttaga
     8701 cataatcaca tctacctggg cacctaattt gacgaattga cttgtaagat cagcgctttt
     8761 gtaagcagaa atacttccag taacgcctag taagatcttt ttcattttga ttcctccagt
     8821 ctaattttct cagtaatcaa tgcagcaatc tcagatttac tttgagcttc ttccactgtt
     8881 ccatctttag ataagagata gccgtggtgt gtctcgccat ggatttccgt caaatcattt
     8941 gcgaaaataa aatcagtttt gttatttatc agactatttt ttgcgactgc cagaagagat
     9001 tcctttggta catctactaa tagtttgaaa ccaatgattt ttgcttttgg ctgccattca
     9061 cgtagataag aaatgatttt tggtgttttc ttcaaaacga gcacaagatg ctcggtgtta
     9121 gaagagatct ttgaaacagt ggtatctgtc tgttcatttt ccgcaaacca attgtccagt
     9181 tcttcttgcg ttgaagaagt gggtaggttt tttgccagct gttcttctga aaaactaaaa
     9241 gcaggagtaa aatcactgac tgccatactg tggataatcg catcgtagtg ttcttttttc
     9301 gttagttctt ctaattgtaa aaataagtcc agtgcagatt cgatcgtata aatcgttata
     9361 ttggagtgtc ttttcggttt cagtgcttgt ttcgtagtca cgtaatcgat tgtggtatcc
     9421 ggattggcag ctaaataatc agctaatgct tgccctaatc gtcctgtcga atgatttgta
     9481 atagcacgta cttgatcgat tttttcagtc gttcctccag ctgtgattag aatcttcatt
     9541 tatggtcacc tacatttact agtgttcttc cttggtgtgt tccagcaagt agtttagatg
     9601 ctgtttcagg caattgatcc agagtaatct cttggacgac cagttcatct gcaatctgca
     9661 aatccgccat ctgttgccat aaagaaagac gttgatctat tggtacattg acagaatcaa
     9721 ttccaatcaa ctggatgttt cgcagaataa aaggcaagac agtcgcgttt agtttgattc
     9781 ccccagcatt gccacataaa gctactgcac cgttataaga gatcaatgga agaagactgc
     9841 ttagctgctc gccacctact gtgtcgatga catagtcgat ttgctgtttt cctaaagctt
     9901 ttgttgtctc tggcagaaat tcatcaggtc gtacgacttg caaagcacct agacttttta
     9961 gccatgtgac agactcttct tttctggaaa aggtaatgat tgactggaat cctagtttat
    10021 gtaacagggc tatcgctgta ctgcctacac cgccagaagc accagtaacg acaacttttt
    10081 tatcggaggt cataccttga cggattaaag cgttcacaca caacatagca gtaaaccctg
    10141 ctgtaccaag gatcatcgct tgacgcagcg tcatattttt tggtagagga actaaccagt
    10201 ctcctggtac tttttgatat tggctataac cgcctggatg ggtgacgcca agcccaaaac
    10261 cagtagcaag tacaagatct tcttttgaaa aacgtggatt gttactttgg acgacagttc
    10321 cggataaatc aatccctgga gtcatcggat atgttcgtat cacctgacca gactcgctca
    10381 ttgccaatgc atctttataa ttgacatcag aataggcaat ttttaccaag acttcatttt
    10441 cagccaactc agtaaatggc agatcactga ttgctgtatg gaaaacaggt tcctttatta
    10501 cctgaaaaga tcgaaaagcg ttcattcgtg agacactcct tttaaaattt tcttttatca
    10561 taccataaag cctactcttt tattcttgtt cttccgtaaa tttagacaaa aattaggtga
    10621 aaaataaaga atcaaacaac tagaatttat tccaaaagat aaagaaatat tcagcaaaaa
    10681 aaattttaca aagggttgcc tttttcaata aatttcgtat actaatacga gtggtagtaa
    10741 acaggaagtt tactattaga aaacttcgat tcggaggaaa ttatggaaat tggtgagaag
    10801 ttaagaaatt tgcgcatcca aaagaatttg acacaagaag aactaggcga acgcacagat
    10861 ttgtcaaaag ggtatatttc ccagttggaa cgagatttaa gttcaccatc aatggaaaca
    10921 ttcttttcta ttcttgaagt cttaggtgtg actccagaag aattttttca tcaagaagca
    10981 gcgaatctgc aagtggttta ttcaaaagaa gatcatacgg tttatttcga tgaagaaaac
    11041 gggtatgaat tggaatggct cgtggctgat tcgaatgaaa aagaaatgga gcctgtcttg
    11101 ctgacttttg aaggtgcagg agaatataag acgtttgaac cttcattatc tgaaacgttt
    11161 atttttgtat tagaagggga attagagtta agcttaggag agacggtcta tgtggcaaaa
    11221 gcaggacagt cgatctatta tcaagcgaca aaacaacatc aattaaggaa tcatctcaaa
    11281 aaacgaacaa aagtattgat tgttgcaacg caatcatatt tgtaatatag ggggtaaaaa
    11341 cgtggggaaa ccaatcattt catttgatca agtagtgaaa cgctatgatg atgaaacagt
    11401 attaaaaaaa gtaagttttg agatcgaaca aggaaaattt tatacactgc ttggtccttc
    11461 cggctgtggt aaaacaacga tcctacgtat tatagctgga ttcaccgaag ctacagaagg
    11521 ggacatctat tttgaaggga aacggttgaa tgatttgcca gctaataagc gacaagtaaa
    11581 tacagtcttc caagattatg cactttttcc tcatatgaat gtttttgata acgttgcttt
    11641 tggattgaag atcaagaaaa tgtccaaatc agatatcgaa aagaaggtaa aagatgcttt
    11701 gcgtatggtc cagcttcctg gatacgaaaa acgggagatt agtgaaatgt ctggtggtca
    11761 aagacagcgt gtagcgattg cacgagcaat tgtaaatgaa ccgaaagtat tgctattaga
    11821 tgaaccgtta tcggcgcttg atttgaaact acgtacagat atgcagtatg aactgcggga
    11881 tctgcaacaa cgattaggaa tcacgtttat ttttgttact catgaccaag aagaagcgtt
    11941 ggcaatgagt gatgagattt ttgtcatgaa taaagggaaa atcgtccaaa gtggtacacc
    12001 cgtagatatt tatgatgagc caatcaatca tttcgttgct gattttgttg gggagagcaa
    12061 tatcgtggac ggtgtaatga tcgaagataa tctagttgag tttgttggta agcaatttga
    12121 atgtgtcgat ggcgggatgc gtctaaatga accagttgaa gttgttttac gcccagagga
    12181 tttgacgatc acgacaccag ataaaggaaa actagtcgta acggtcgata ctcagctgtt
    12241 tcgtggtgtc cattatgaaa tcatctgtta tgatgaacag caaaatgaat ggatggtcca
    12301 ttcaacgaaa aaagctaaag aaggaagcaa agtaggattg gcctttgagc cagaagatat
    12361 ccatgtcatg cgtttcaacg agtctgaaga agaattcgat gcacgtcttg acagctacga
    12421 agaatagggg ggacaagaat gaaaacaatg cgcaggatct attccgtccc ttatattttg
    12481 tggctcggtt tattcgtgat cgcgcctgta ttaatgatca tttatcaatc tttttttgat
    12541 atgaatgggc aattcacgct gaataattat ctatcttatt ttacgtcggg gacatcgctt
    12601 tctatgacga tcaattctgt atggtatgca tttttgatca cgtttttcac gctgatcatc
    12661 agttacccga ctgcttattt tttaacaaaa ctgaagcaca agcaattatg gctgatgctg
    12721 attattttgc ctacatgggt gaatctgctg cttaaagctt acgcatttat tgggattttc
    12781 agtgtgcacg gcagcgtgaa tcatttcttt gattttatcg ggatcgggcc gaaacagata
    12841 ttattcacag acttcagttt tctgttcgtg gcaacgtata tcgagattcc atttatgatt
    12901 atgccaattt tcaatgcatt ggaagagatg aatccttctt tgatcagtgc tagtagagat
    12961 ttaggcgcga atagctttga gaccttccga cgagtgattt ttccgttatc attaaatggt
    13021 gtgaaaagtg gtgtccaagc agtatttatt ccttctcttt ctttattcat gctgactcgt
    13081 ctgattggcg gaaatcgggt gatcacttta ggaacggcta tcgaacagca tttcttggtt
    13141 acacaaaact gggggatggg ttctacaatc ggtgtgattt taatcattgc tatgttcatc
    13201 gtgatgctcc tgacaggcga gaagaaaaaa ggaggtcgct cataaatgaa aaaatttaaa
    13261 tggtcgtatc tttatttagg gatcgtcttt ttattcttat atgcaccgat cttctatctg
    13321 attttttatt ccttcaatga aggaggaacg atgaacagct ttacaggatt tacttgggaa
    13381 aattatgaag ctgtttttga agatacacgt ttgatcatta tcgtattgaa tacgttcatg
    13441 ctggctttct tatctgcatt gatcgccacg atcatcggga catttggggc tatgggaatc
    13501 tattacacga aaaaacgacg tgcacggaca gcgttgttga gcatgaataa catcttatta
    13561 gtctctcctg atgttattat cggtgctagt ttcttgattt tctttacact actaggattc
    13621 ggattaggtt ttcagagtgt attgctttcc catattgctt tcagcattcc aatcgtcgtc
    13681 ttgatggtct tgccaaaatt gcaggaaatg aacgattcaa tggtcgatgc ggctagagac
    13741 cttggtgcta ataatttcca agtagtcaaa aatatcattt tgccttatct ttctccagga
    13801 atcatagctg gatattttat ggcatttacc tattctcttg atgattttgc ggttacattt
    13861 ttcgtcacag gaaatggctt tacgacgctc tcagtagaga tctattctcg tgcaagacaa
    13921 ggaatcagtt tagaaatcaa tgcgctaagt gcgttagttt tcttgttctc catgatattg
    13981 gtcattggat actactttat tagtaaagaa aaccgtccaa aacggctgaa gaaaaacaga
    14041 agagtacgcg agggggtggc acggctgcga tgaaaaaatt acaatctctg tttttaggta
    14101 ttctcatcat tattgcggtg ctgctgcttg gtgttcgtca acttgaaaaa acaagtggca
    14161 tgagtggagc aaaggtactt accatctata actggggaga ttatatcgat cctagtttga
    14221 tcacgaaatt tgaaaaagaa tatggataca aagtgaatta tgaaacattt gattctaatg
    14281 aagcgatgtt cacaaaaatc cagcaaggtg ggacaaacta cgacatcgct ataccaagtg
    14341 aatatatgat ccaaaaaatg atcaaagaaa aactagtact tccattagat cattcgaaga
    14401 tcaaaggatt aaataatatt gatccacgat ttttagactt ggattttgat ccagataatc
    14461 agtattcgat tccatatttt tggggaactt tagggatcgt atataacgat aaattctttg
    14521 atgcaaatca aattaagcat tgggatgatt tgtggaagcc ggaattaaaa gacagtttga
    14581 tgctgattga cggtgcacga gaagttatgg ggctttcttt gaatagttta ggctattctt
    14641 tgaatagtaa gaatatggca cagctgacag aagcagcaaa taaattgaat catttaacac
    14701 caaatgtcaa agcaatcgtg gcagatgaga tcaaaatgta tatgatcaat gaagaagcct
    14761 ccgtggcagt gacattttct ggcgaagctg ccgatatgat gtgggaaaat gaacatttac
    14821 attatgtgat cccaccagaa ggatccaatc tttggtttga taatatcgtt atgccaaaaa
    14881 ccgtgaaaaa taaagaaggt gcctatgatt tcatcaactt tatgctagaa ccggaaaatg
    14941 cggcacaaaa tgcagagtat attggttatt ctacaccaaa tgaagaagca aaagcgttat
    15001 tgccaaaaga catttcgtcg gacgaacaat tctatccatc tgatgacacg atctcccatc
    15061 ttgaagtcta caaagatcta ggatcgaaat acctaggcat ctataatgac ctattcttag
    15121 aattcaagat gtataggaga taatggcaaa actcagtaag aatggggact tatcaacgtt
    15181 ctaaagagct aaaaatcgtc ctttttctat ctgagttttc caatcttcta ccaaaagcgg
    15241 atgaaacgcc gctttttttg tgttatttta ttttttctac cacgtttttc taccagagaa
    15301 aagtggacaa aaccattgta aaatagggcc cgagacgggc tagtaaatct aagattgaaa
    15361 aagttacaga aggataaagt aattggaaac tatgcaaata tacttaaccc ttctgtaatc
    15421 tattaataaa actaatatat gaatccagtt caatatttgt agctatgaaa aaacataaaa
    15481 atcatattag ataaaaacta aattggtaga taaattattt tgtcagatat ctgaggcaaa
    15541 tattcgacta tttgtgtttc aaagtaaaaa ttggaataca aatatagaat agacatattg
    15601 cagccgatag tttattatgc ttttagacac attaaactgg aggaaaaaat gaaaaagagc
    15661 gctaaagatc gagtttttga tgagctgata cgactaacag ggaatatgtc ttctgtcact
    15721 gccgagaaaa tttcgaagaa tctaggtatc tctcgtcaga atgccagcca ttatttgacg
    15781 agattagtgg aagataaaaa ggtcgagaaa ttaccgggaa agcctgtttt gtggaagcct
    15841 ttagatgaat atgcggttgt agataatacg gagcaaataa acgaagcctt tcattctgta
    15901 gttggtcatg acggaagtct gagagaggtc attcaaaaat gtatagccgc tgtcaaatat
    15961 ccgcctaacg gactcagtgt attaataaat ggagccacgg gtgttggaaa aagtttttta
    16021 gccacaaaaa tttttgaata cgctatccat gagcaaatta ttgaaaaaga tgcaccattt
    16081 gctatcttaa actgtgccga ttatgcggac aatcccgaac tgctttcggc aacactgttt
    16141 ggatacaaga agggggcctt tacgggggct gaaaaggata ccgatggttt attggcaacg
    16201 gccaacaacg gctatctttt tctcgatgag gtccatcgcc tatcgaaaga aaatcaagaa
    16261 aagctctttc tatttattga tactggaaac tatcgtccag tcggtgaaaa tgtaaattgg
    16321 cattctgcta aggttcgttt tattttcgcc acgacggaaa aaggtgaaaa ttatttatta
    16381 gacacctttg atcggcgtat tcaaattagt gttatgttgc caacttttga agatcgcccc
    16441 attcgagaac gattagagct gattcaatta ttttttcaaa atgaagcgga tgttttacaa
    16501 aaagatatta ttgtttcaag agaagccttg tctttaatgt tatctcatcc ctttagtggg
    16561 aatattggta agctgaagaa tatcattaaa atcagttgtg cggatgtgta tagtcgaaca
    16621 caagaagaac cattaacgat tgggaaaaat gagatcttga ttcagttaaa tatgctggaa
    16681 tccgaagtag aaagcttgcc gattgctagt ttattgataa agaaaaatca aaagataaaa
    16741 acggatcgaa tcaaaccgtt tgattatgat tcgattttgg aaaaaattac tcagctgata
    16801 aatgaaggta atttcaatga aaatttttct tcgataaaga atgaagtaca gcatttggtg
    16861 catcacattg ataaggattc ttttttatca atgagtagaa aattgatgat tcagatgttt
    16921 agaaaggtct gggtatccgt tttaggaaaa acgtacggat tgacgacgag tggacctgtt
    16981 gcagaaatta ttagcagatc ctatgtcatt tccccaagct ataagacaga cttaaccggg
    17041 ataaatcgta cattagctgc aaatctacct cggacgactt acttatgtaa tcagttcttg
    17101 gagcatttac caccgctctc agaaaaggac gagaactttc taagactgct tattgtcgtg
    17161 actttatctg attatgtaga tgaatccatc gagctgaagg gcctgttgct tgctcacgga
    17221 gatagtacgg ctagcagcat tcaagcagta gtgaatcaat tatgcggcaa ttatgttttt
    17281 gaagcgttgg atatgccagt agagagtaat gtgtcagaga ttgtcgaatc gacaaggaat
    17341 ttcgtgaaac ggcaagtgaa tacagatagt ctagtgctga tcgtagatat gggctcttta
    17401 aatcaaattt acacacagat caagaacgat ttaaaagggg agttacttct tctgaataat
    17461 ttgacgactt ccattgcact tgatattggc ttgaagatga cgatgaatgc gccatttaaa
    17521 cagattgcgg aggatgcagc agacaagtat tcaatcaatg ttcaatactt tgaaggcttt
    17581 tctcaaagta gcaacattat catttcctgt atgtcgggtt tggggatttc ggacaaattg
    17641 aaagatgttt ttcgtcatca tttatcggat gaatccatcg aagttttcac gaaggactac
    17701 agagaactac gtgaattaat cgaccaaaat gatgaaactt attttgacaa gacaaaattg
    17761 gtcatcacga cttcggatct tccacgttcc ttttcaattc ctaatattaa tgtttatgac
    17821 atcttagacg gtgatggagc agccaatttg gagcatattt tgagtcctga aatgaagccg
    17881 gcagaattta accgcttgat tcaggagcta gtaaaattct tttcgttgga aggaattgcg
    17941 gaccgtttga actttctgaa cccaacgatc gtgatgagtg aagtggaaac agtcatttct
    18001 aaatatgaaa attactatca tttcacttta aatggccgtg taaaactaaa tctctatatg
    18061 catacggcat tgatgatgga acgtttattc ttggctcgga cagaaaaatc tgaagaaccg
    18121 aattatgatt tgaaaccaga tgaagatgaa ttttattatg tttctagaag tatttttcaa
    18181 tcaatggaga tgaaatataa ttttaaagtc aatgcttat
//
LOCUS       DAZKLA010000050        17816 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000050 DAZKLA010000000
VERSION     DAZKLA010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.050"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..961)
                     /gene="murB"
                     /locus_tag="ACG85N_001467"
     CDS             complement(26..961)
                     /gene="murB"
                     /locus_tag="ACG85N_001467"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFQ0224241.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1034..1954)
                     /locus_tag="ACG85N_001468"
     CDS             complement(1034..1954)
                     /locus_tag="ACG85N_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0224242.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2067..2819
                     /locus_tag="ACG85N_001469"
     CDS             2067..2819
                     /locus_tag="ACG85N_001469"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFQ0224243.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3683..4216
                     /locus_tag="ACG85N_001470"
     CDS             3683..4216
                     /locus_tag="ACG85N_001470"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFQ0224244.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4706..5230)
                     /locus_tag="ACG85N_001471"
     CDS             complement(4706..5230)
                     /locus_tag="ACG85N_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFQ0224245.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5223..5696)
                     /gene="tsaE"
                     /locus_tag="ACG85N_001472"
     CDS             complement(5223..5696)
                     /gene="tsaE"
                     /locus_tag="ACG85N_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFQ0224246.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5819..6799)
                     /gene="pta"
                     /locus_tag="ACG85N_001473"
     CDS             complement(5819..6799)
                     /gene="pta"
                     /locus_tag="ACG85N_001473"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFQ0224247.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6952..7632)
                     /locus_tag="ACG85N_001474"
     CDS             complement(6952..7632)
                     /locus_tag="ACG85N_001474"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0224248.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7758..8627
                     /locus_tag="ACG85N_001475"
     CDS             7758..8627
                     /locus_tag="ACG85N_001475"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0224249.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8653..9168
                     /locus_tag="ACG85N_001476"
     CDS             8653..9168
                     /locus_tag="ACG85N_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFQ0224250.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9215..9778)
                     /locus_tag="ACG85N_001477"
     CDS             complement(9215..9778)
                     /locus_tag="ACG85N_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0224251.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9833..10012)
                     /locus_tag="ACG85N_001478"
     CDS             complement(9833..10012)
                     /locus_tag="ACG85N_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224252.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10089..10598)
                     /locus_tag="ACG85N_001479"
     CDS             complement(10089..10598)
                     /locus_tag="ACG85N_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224253.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10694..11074)
                     /locus_tag="ACG85N_001480"
     CDS             complement(10694..11074)
                     /locus_tag="ACG85N_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0224254.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11404..12153
                     /gene="pnuC"
                     /locus_tag="ACG85N_001481"
     CDS             11404..12153
                     /gene="pnuC"
                     /locus_tag="ACG85N_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFQ0224255.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12474..13691)
                     /locus_tag="ACG85N_001482"
     CDS             complement(12474..13691)
                     /locus_tag="ACG85N_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0224256.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(13949..15226)
                     /locus_tag="ACG85N_001483"
     CDS             complement(13949..15226)
                     /locus_tag="ACG85N_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224257.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(15305..15949)
                     /gene="pcp"
                     /locus_tag="ACG85N_001484"
     CDS             complement(15305..15949)
                     /gene="pcp"
                     /locus_tag="ACG85N_001484"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFQ0224258.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(16148..17092)
                     /locus_tag="ACG85N_001485"
     CDS             complement(16148..17092)
                     /locus_tag="ACG85N_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFQ0224259.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(17094..17780)
                     /locus_tag="ACG85N_001486"
     CDS             complement(17094..17780)
                     /locus_tag="ACG85N_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFQ0224260.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         5104 a         3663 c         3212 g         5837 t
ORIGIN      
        1 tacttatttt aagttccatt acttattaat tggctaaact tctgcttgtt tctgatattg
       61 cttgtttttc tccaatgatc cgcacttctg tttctaaaga cacatcaaat ttttctttga
      121 ttacttcttg gatgtgcgca atcaattcaa tataatctgt cgctgtcgca tgatcgatat
      181 tgacgatgaa tccagcatgt ttttcagaga cttgtgcacc gccccatttc aggccttgca
      241 atcctgcttg ttggatcagc tgacctgtat agtgtcctac tgggcgctta aagacacttc
      301 cgcatgaagg gtattctaat ggctgttttg attgacgcaa ttcggtcaac tcatccattc
      361 ttgctttgat tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc aatacgatag
      421 cacgtaattc ttggacttta ctgtgacgat aagaaaatac catttcgtct tttgtcaaag
      481 ttttcaatgt tccatcttca agcaaaagat ccacttcagc aaaaacatct ttgatctctc
      541 cgccatacgc acctgcattc atataaaccg ctccaccgac acttcccggg atgccgcaag
      601 caaattcaaa tccagtcaat gattctgcca aagctgcata agtcgtatca atcagtgagg
      661 cacctgcttc cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt tcagtcagca
      721 tgatgactac accgcgaatc ccgccatctt gaacgatcag attgcttgca tttcctaata
      781 ccatccaagg tatatcatgg attcggcaat aatcgatgat ttgctttact tcttcttgtt
      841 ttttcgggaa tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta tagttcatca
      901 atggttcatc aaacaatata gtaatcgtgg ctaagctttc aactatttct tctttattca
      961 ctgaggattt tcctttcttt tctcaaacgt cctcattatt ctatcacgct taatacaatc
     1021 ttgccagtct tgttcaaagt tgatgacggt agtgatcttt aagcacttcc tcacataatt
     1081 catgacttct tacaacttct ttttgtttaa gcaattctgt ttccgattta cctaaacgga
     1141 tctgagaaaa gaaggcctct accattggct caaacccgcg tttgactagc gtgtttgtcc
     1201 agtcgccaaa ttctttcact ttttttccat cagcatcttg aatgacaagc tgggtcagat
     1261 cgtttacttc ataagtacca tttttacttg ttacttgata tttttctata ttagcgccac
     1321 ttgctaaatc catcgttaaa atagccgttt gattttgtgt ttctaattgt aaaatggcat
     1381 attccaaaaa ttcctttgtt tctcttatct ggtattttgt tctcaaaatg ggttcatcta
     1441 ataaatacac agctgtatca ataagatgta aaaacaaatc ataaaggacg aactctgtcg
     1501 tttcttttgc cgctattcga tttttttgca ggtggatcaa ccgcttatct gggatacgtt
     1561 tcaattcttc taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa attatttggt
     1621 tcttatcagc taattcttgc aattcacgaa cttcatttag atggacactc agtggtttgt
     1681 caatataaac atggatacca ttttctagac attttttggc taattcataa tgtacttttg
     1741 ttgctgcgtg gatcatacat gcttcgattt tttcttctaa aagctcatcg atcgtttcat
     1801 atagatgttg gaacccgtac tctttttgaa ttctttcttt tgtttcttta tttcttgttg
     1861 caaaataaaa atcaagtgtt ccttgttttt tcgcataagt tggtaaatat gctttttctg
     1921 cgatattccc tacaccgatt acaccaatct tcatctactt tcctcctgga tcatttagtt
     1981 actttcatct tacaccattt tttactaaaa tgaggtacaa tagtatcgca aaggtaagga
     2041 aattaattcg aaggagtgtg cttttattga aatttatctc ttggaacgta aacgggcttc
     2101 gtgccatcgt aaataaaaat tttttagaag tttttcatga gttagacgcc gattttttct
     2161 gtctgcagga aacgaaactc caagcaggac aaattgattt agatcttcca ggttattatc
     2221 aatattggaa ttacgcggaa cgaaaaggct attccggcac agcgattttt gctaaaaaac
     2281 cagcattgaa tgccacttac ggaatgggta tcgatatcca tgatacagaa ggccgtctga
     2341 ttactttgga atattccgat ttctttcttg taacctgtta cacgccaaat tctcaaagcg
     2401 aattaaaacg tttagattac cgattagaat gggaagaagc cttttataat tatttggaaa
     2461 atctaaaaaa acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct catcaaaaga
     2521 ttgatttgaa aaactggaaa acgaatcaaa aaaatgctgg attcactcca gaagaaagag
     2581 cggctctttc tcgactgttg gataacgggt ttatcgatac attccgttat ttttatccaa
     2641 cccaggaagg tgtctactct tggtggaact accgattcaa ttcaaggaaa aacaatgctg
     2701 gttggcggat cgattacttt ttgacgagca aagatttaga gccaggacta gctgatgcaa
     2761 agatccatac ggacattatg ggcagtgatc attgtccagt agaattagat ttgaaataaa
     2821 gaggttgtga aaaagtcgtt tagtttcgag tattaagaaa aattttgtga aaatggcttt
     2881 ccacattttg cataaatttg gcataatacc gagaaactgg cttttgaaca ctgtttatcg
     2941 agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3001 ctcatatttt tcgtttggtt gggcttatta ccgcagatac aagcttttga acaccgttta
     3061 ttaagaggtt gtgaaaaagc tgcactgcat caagtaataa gaacagccaa acaaaaatag
     3121 ttcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
     3181 ttattaagag gttgtgaaaa agctgcactg catcaagtaa taagaacagc caaacaaaaa
     3241 tagttcctca tatttttcgt ttggttggac ttattaccgc agatgcaagc ttttgaacac
     3301 cgtttattga gaagttgtga aatcagcgtc ctgacctgag tattagagaa acaaatggca
     3361 aaatagttcc tcatattttg ctattttttg agctaatacc gagggtcagc tgattgaaca
     3421 ccgtttagtg gagaagttgt gaaaaagtcg cctactttcg agtcgtaaga aatttttttg
     3481 aaaatggttc ctcacaactt tgcataaatt tgacttaata ccgagaagtc atgatcccca
     3541 tctaactcca aatcacaagg aatgagttca aaaactagct tttctcaaca aatattacat
     3601 aaaaaattct atgttttttg ttaaatttat ttgctttttt caaaagtatg ttacagtaat
     3661 ttcccaagga ggttttacag aaatgaattt tgttgccatg gattttgaaa ctgccaatca
     3721 tcagccttat agtgcttgtt ctcttgcgct agtcatggta aaaaatagcc agatcgttga
     3781 tgaattctat acattgatac agcctgagac tccttttttc tggcgaaatg tccaaattca
     3841 cggaatccat caagaagacg tacgcaatgc gccgaaattc ccagaagtct ggcaaactat
     3901 ccaaccatat tttcaaaaaa atcgtttagt cgttgcacat aatgctgctt ttgatacaaa
     3961 ggtcttagcg ggatgcttag attattatca tcttgaacag cctaattatc tttctttatg
     4021 tacagtgaaa agtagccgtc gcctgtttcc tgaatttcca aatcatcgat tgaacactgt
     4081 gtgcgaaaat ttgaacatcc aattagatca tcaccatgat gcattggaag atagtcgtgc
     4141 atgtgcggaa atcctgcttt atcaagaaaa aaaattcggg acagatccat tgaaaaaact
     4201 tgtgactata aagtaaaaga atacttatga aacacacaaa gaggtcgtga gcctgccgtt
     4261 tcactccgag tcacaaggag caatttcaaa aaatagcttc tcatgtttat taaatattgt
     4321 gactggtgac cgaggagctg gcaggagaac accgtttatt ggagaggttg tgaaaaagct
     4381 gttctgcatc aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcatttgg
     4441 ttggacttat taccgcagat gcaagctttt ggacaccgtt taccaagagg ttgtgaaatc
     4501 agcgttttga cctgagtatt agagaaacaa acggcaaaat agttcctcat attttgccgt
     4561 tttttgagct aataccgaag gtcagctgat tgaacaccgt ttattcaaaa aaaatggact
     4621 gcgacacgac ttttgtcaca gtcccttcgt gtgtttttct gccttctcaa aaggatgaca
     4681 gtttctcact cttttcctat ttcttctatt caatcaaata actcatttta tatacatcta
     4741 aaaattcacc tagatctgtt cttgcccctc taggcatgac cgcttcgatt tggaagccca
     4801 tttttcggta aaggtgtacg gctctttcat tacgaacttg aacgtcaagc tccaatcgaa
     4861 aaagtacacc catttccttc gcccaactga tgatttcttc tagcataaga gtgcctagtc
     4921 ccattcccca atattcctgc agaatactga taccgacttc tcccacgtga gacaaacgaa
     4981 attgactgtc tgccttgaca gatgctgttc caataatcgt cccttcatag attgcaagca
     5041 gcaataaatt attattgctt tcgtatatat aatccaactc ttcttctaag gtttctggtg
     5101 gtaacaggag ttctgcttca tccaaaacta aaaattctgt ctccttatta acttgtccca
     5161 tcatcaaaag aatgtctttt gcatctcctg gcactgcttc ccgcaagatg atcccaactt
     5221 cttcactcat gattttcagc ccatttttct agaattcgct gtttgaaggt ttcactttgc
     5281 tccccgtatg agattagttt cacataccgc ttttctaaat ctgtatcact tttttctaaa
     5341 atcagttcca aataatcctc cggcagtgct tcttctaata gatttcccca ttcaacaacg
     5401 gaaagcccat caccttcaaa atattcatct aaccctaaat ctgctccgct agctgctacc
     5461 cgataaatat ccatatgata caacggaatc cgaccttgac tatattctct aataatcgtg
     5521 tatgtcggac ttttgatcat ttgcgttatc cctaatcctt gggcaattcc tttcgtcaac
     5581 gttgttttac ccgcacctag atcaccagtc aatatcagat tgtcacctgg cattgcggaa
     5641 tgaccaattg cctctgcgaa ttgttctgtc attgttaatc ctgatagttc gatcattttt
     5701 catcttctcc cgctcttctt tctttttgcc agtatatcgc gtaatgaagt ttttttaaag
     5761 aaaaaatgaa ctagtcaaga acagcagctg ctgtttttgg ctagttcgcg tgtatttgtt
     5821 aatcaatcaa tgtttgtgct gctgtgataa tcgataattt atatacatct tcttcatttg
     5881 ctccacgaga caaatcagaa accggtttgt tcaagccttg tagaattggt ccgattgctt
     5941 caaagttacc gaaacgctga gcaattttat agccgatatt tcctgactgc aattctggaa
     6001 atacaaagac tgttgcttga ccagctactt ctgaatctgg cgctttcagt tgtgcaactg
     6061 aaggaacata agaagcatca aattgcaatt cgccgtcgat catgtattgt ggcgctagtt
     6121 ttttagcgat tttagttgct tcaactactt tgtctacttc tggcgatttc gctgagcctt
     6181 ttgtcgagaa gctcatcatt gctacttttg gttcgatatc gaacaattct gctgtttttg
     6241 cagaatctac ggcgatttca gccaattctt gcgcagttgg attcacgttg atcgcacaat
     6301 ccgaaaagac atatttttct tgatctcttc cacgtaccat gatcatcgct ccgcttgtcc
     6361 gactgatgcc aggctttgtt ttgatgatct gaagtgctgg gcgaacagtg tcgccagtcg
     6421 aatggatcgc tccagagacc attccgtctg caatccccat atacgtaagc atcgtaccaa
     6481 agtaattcac atcttttaaa atcttttctg cttgttcttt ggatgccttt ccatttctac
     6541 gttcaacaaa agtatccacc atttcatccc acttatcgta attatcaggg ctgatgatcg
     6601 taaagctaga tgttttgatt cctctggcat gcgctgcatt tcttatttct tcttcgttcc
     6661 ctactaaaat gggttccaca agttcttctg ctttcaaccg ggcagctgcg cctaaaatac
     6721 gagcatcagt agcttccggg aaaacgattt tgatcccacg gcgaacaatt ttaaatctta
     6781 aactatcaaa caattccaca agatgacctc cagacgtttt tatatgtatt atcatacact
     6841 attccagaaa aaaagaacag tttattttat ctgtatcagt caaaaatgct ggaaaaatta
     6901 agtttttcac atacaaagcg aactgcacta taacaggaaa gcgctgtttt attacactgt
     6961 ttcaggcagt tgccaatcaa tcggttcttc tcctaacgca aggagcgcat cattcgtttt
     7021 cgagaagggt ttggaaccaa aaaagccacg acttgcagat aacggactgg gatgtggaga
     7081 tgttatgatg atatggcgac tcttatcgat cattttgatt ttttcttgtg ccggttttcc
     7141 ccacaagata aacacgattg gtttttcccg ttcatttaat ttttcaatga tcgtgtctgt
     7201 taatctttcc caacctttcc cgcgatgaga atacgcttgc ccttcccgaa cggtaagaac
     7261 ggtgttgagt aaaaggactc cctgtttagc ccatgacact agatttccat gttgaacagg
     7321 tgcgattcca agatcatcat aaagctcttt atagatattt cttaacgatg gcgggatctt
     7381 tacgccaggt tgcacagaaa aagataagcc atgagcttga ttttctccat gataaggatc
     7441 ttgtcctaaa atcaccactt taacttcttc ataaggagtc aattccaatg cttcatagat
     7501 atgatacata tctggatgga tcttttggga cgcatattct ttttttaaaa attctcttag
     7561 atgcagataa taatcttttg aaaattctga ggacaaaact tcttgccaac tgttatgaat
     7621 gatttctttc aaaaactcca cctcctaata aaaagaataa ctcacatttt cttcaatcac
     7681 aagggacaat cttcaccctc agcacttgat taatatggta aactaggaac aagaagcaga
     7741 aaggaacgat aatcattatg atcaaattaa ttgcatcaga tatggacggc acattattag
     7801 atgcccatat gagtgtttct cctgaaaatg ccgaggcgat acgttttgca aatgatgccg
     7861 gtgttgagtt catggtcgct actggtcgaa actatcaaga agctcgcgca gctttagatg
     7921 aggtaggaat cgattgtgcg atgatcactt tgaacggggc acaggtattt gataaagaag
     7981 gaaattcatt gtttaccgtg tcgattccaa ataatcaagc aatctctgtt ttagatattc
     8041 tagatgctaa cggtatttac tatgaagtag caaccaacga tggtctttat tctgaaaatc
     8101 aagcgaaacg aattgaaagt tttgcttcca tggtggctac ccatttacct caccttacgt
     8161 ataaaatggc gattgccatg gcctcagcta agctggagtt attgcacatc acatacgtgg
     8221 acagtattcg tgctattttg gaagaaaaga atctggaagt tttaaaaatt atttgttttc
     8281 atacagaagg gccagctgta ttaggtccag ttggaaagca gatttctctt ttaggcgatc
     8341 tagccgttac ttcctctggt caaaacaata tcgaagtgaa ccataaaaac gctcaaaaag
     8401 gaattgctgt tgcccatgtc gctcacgagc gtgggatttc tttagaagaa gtcatgacga
     8461 ttggcgataa cttcaatgat gtcagcatgc tgcaagtagc aggagtcagt ttcgcaatgg
     8521 gaaatgcaga aatcgaagta aaagattatg ccaaatatac aacggacaca aaccttgaat
     8581 ctggcgtagg aaaagccata ttgcgcgcga ttgcagaaga cttgtagttt tatctagaaa
     8641 agagggatat agatgtcaga atttttcatt caggaaaagc agcttggacg tgcaactcgg
     8701 acaattgtca aaaacgaaaa aggacattcc ctgtttttga tggttggtcg ctggggaaca
     8761 aagggcgatg cattgtctct ttatgccatg aatggcgatc tcgttgctca catcaagcag
     8821 ataagcctga cattcggtac gcgttttgag ctgtataaag agttcgaaaa ggtgggaacc
     8881 atgcagaaaa tattcaattg gcccggtgac ttttattata ttcgtagact tcactggaca
     8941 gtccaaggtg atatctataa tcatcaatat caaatccatc atttcaatca gcaaatcatg
     9001 aaaatggata aagcaacact tttttcagga gattactatg tattggatgt ctcaaatgat
     9061 gaagatgctc cagtttgtat ttgtattgct gcggttttag attactggct ctaccgtaaa
     9121 gacaaaagtc gcgataagcc tgtttcttac cgacttaact taagttaaaa agatgtacct
     9181 gaaatgcgga aatacgcatt tcaggtacat ctttttagat attcttttgc atatgatcat
     9241 aaagatgacc actgatcatc cgctgaccga caactttgaa gtcatggcgt tcgtataatt
     9301 ttttagcctt tggatttttt tcatccacac ttagaccgat gacggagcga ttagctttct
     9361 tcgccaattt aggtaatgct tctaataatc gcgaaccgat gccttgtcct ctaaaatctt
     9421 ctgatacact gatagagtcc aaataccatt cgttgggaaa cgtttcttta tcaatgaaca
     9481 aacgtgtaga cgcatctagc tgatatttct ctaagatagg taccagtggc tgatcgatga
     9541 ttgattcttc ttcatctgta taaccgtaag caactcctgc aactttaccg tctatttctt
     9601 ctacgatacc tcgtgaataa ctgtaacgat aatttggatc agtgattgct tcttctaaaa
     9661 tctctaaagt cttttgttcc cctgtctgct tcaaaaatgg cagttccata tcttttagaa
     9721 tcactaagat caatggggcg atttctggag catcttcttt tctcgcaaaa cgaatcatag
     9781 tttcctctcc ttttctccat tctaacatgc agaaaaagaa atcgcaaaat cgttatttta
     9841 aaaaatcttc aatcttttca gtaatgaaat aaccatgagt aacgattctt gccgcttcag
     9901 ttcggcaata agggcagacg actggcattt ctttatcatt atgttcgact acattattga
     9961 cttcttcgta tgcaaatttt tttccgcaat tagggcattt cattttttcc atgttatctc
    10021 aacttccttt catctttact ctcttagctt actcttatat tgtaaaaaag agaagcaata
    10081 tccttcttct atgcagatat ccatacctga cggtcctgtt ctccatctat taatcggact
    10141 ttataataag gacgttcatc tttgactaga agtgtccact ctttcacttt tgcttttttc
    10201 ccgtagtagc tgactgcttc cttcatcgct gcttgcggtt gtttgaactc ttccgttttt
    10261 tctatatcaa aaagatgttc tgcggccttt tccgtgtcat ttcgtacggt tgttttccca
    10321 gtggatggat tgactgctat gacttgtcct tcacaaaaaa actggtattc atatgttgca
    10381 ttcgtaggat tctccaaatc tttttcaact gcaaaatgaa tagcagatat gttatgttct
    10441 tttgtttcgt tttttacgat tgctgctgct tttttcaatg agatccgata ttcctggtca
    10501 ctaatatcat ccatgtgaaa agcggcattg agcagtcccg tcataccggt aatccctatt
    10561 acgatgaggg cagcaacacc aaacatcatt tttttcattc ttcattctcc tgtttctttt
    10621 ttttaaagaa tatcaagaaa tgaacgaatg aaaaatcgaa tgaaacgaaa aaaaagtgac
    10681 aattttgtta tctttattcg tgcagttcta atggcaatcc gtctggatca aagaaaaacg
    10741 tcattttttt tcctgtaaag gtatcttctc taatcggttc tgattcgatc cctaaggact
    10801 gcaattctgc aattgtttct tcaatatcgt ctactttgaa agcaagatga cgcaagccac
    10861 aagcttctgg atagtttggt cgttgtggcg cattagcaat tccaaaaatc tccaattcag
    10921 cacttcctag tttcagatct aatttatagt catctcgttc tttgcggtaa ttttctctga
    10981 ttacttcaaa tcctaattta ttaacataaa aatcacgtga tttctgataa tctgaaacaa
    11041 tgatggctat atgatggatc gttgtaaggt tcatatgagc actcctattc ttcgtttaag
    11101 ctcggatcta tattaatctc cagttccatt gtaaatgtgt cagattgaaa aaaatagctg
    11161 tttttccttt ttataagaaa aaaggcaaaa aaaacagaaa gtaggtcctc caagacactc
    11221 ttcctgttcc caatataata aatgaaagta agaaattata ttttcaagaa aacaaacaga
    11281 aaattatcat aacacaaatt atttataatt gaaagactag ttttttttca agaaaagcat
    11341 acaatagatt gtgttagtaa atacagaaca cactacattt agtgtcttaa ggagtcctca
    11401 tacatgaagt catattttca gtttttagct catcagctaa aaggctggcc gcaacaaaat
    11461 tattatcttt tctttttcag tcttggctgc caggtcatga ctcttgtcaa tagtcccatc
    11521 acttggctgt ccgttattac atttatcggt accacgcttg gtgtattgtg tattttatcc
    11581 atcaatgcgg caaaatcagt caacggtatt ttaggtattt tgtctgcgat ttgttttatt
    11641 atcgttgggt tttcagcaaa gaactattta agtatcggtg aacagattgc ttatatgatc
    11701 acattggata tccccgtatt gttaagtaaa gaatggaatc ataatatggc ttcaaaaatc
    11761 cgaaaattca ctggaaaaag ctggtttatc gcaatcatca gtactttcct tgtctatctt
    11821 gtttcaggat tcctgattgg tcgttataca gatgatcctc ggccgtgggt agatgccatt
    11881 gctttttcta ttagcttgac tgctggtatc atcagtttct tgcgttacaa caaccaatat
    11941 tactggtggt tagcttcagg gcttgctcaa atggtccttt ggtatatttc atttacgcat
    12001 ggatctgcaa gtttggcgat gttcgtgaac agctctgttt acttgctgaa tgatgtcctt
    12061 gcatttaccg tttcaccttg gtataaccaa aaagaacgtg aacgtatgca aaaaatggaa
    12121 gaagaatact acaacgcaca acaacacgca taaaaaaact gagcgtggga caaaagtaaa
    12181 gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaatcagaag caactccttc
    12241 ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattt cttcttattt
    12301 cttggagtta aacgcttctg tcccagcctc tatttcctac gattagtcaa gtaaaaggtg
    12361 caagaaaaat agctcgtaaa cgaaagcgac aaaatcaaag cacaccaaat aaagccccgt
    12421 tcgtaaaaaa ttttcgatct gggcaaagaa gtggtatact gtaagtggtt tagttaatgg
    12481 tccactgaga ccgcgtacgc atagtcgtac tctgtatgtg ccctaaccat agcgtgcata
    12541 catttcagaa gcttgttcat acaagcaact gtggcaacct tatctttctt agggataggt
    12601 tgctttttta atttgtagta ataatcgaca atatgattag gagctgcgcg ttgctggcga
    12661 atcatatttc gaatggtgaa gaaaataatt ttccgtgctt tgggattacc acgcttattg
    12721 atgcggtctt ggccagtata cttacctgaa tgataccgtc ggatgtcgat tccgacaaag
    12781 gcattcaatt gtttatgggt tttaaatcgt gtgatatctc ctaattcacc gagcaataaa
    12841 gcggctgtga gtgcgccaat cccagggaac gtttggtaca acaaaaagca aggagagcct
    12901 tcggcttttt tgatcatttg tgtcgccagt gctttttttt gttcgagtaa atcttggaga
    12961 agttcggcgt aatagatcgt tttttgacag tggatactgt ctttttcaac agctggatag
    13021 gcacactgtg catagtggat gatttgatct gccttttgct cggcacgatt cgcggagatt
    13081 tttttccgtg tttcgttgat cagtttattt tttatttttg tccgagtaga tgccaataca
    13141 aaatctggat ggggaaaaag acggatcaat gtcaatgcgt aaggggtcag cctgtttgaa
    13201 aagaactgtt caagctctgg aaaagtcaat tgaaggcagt tgtgtaaatc catgcgcaga
    13261 cgagtgattt tcttctcaat ttcttgataa aaacgggcta aatcgcgcat ctctaagtaa
    13321 aaattgcttt gtacttcttt tggctgacgt tgatttttaa gatgagtctg tgcgagccgg
    13381 tgggcatccg atttatctgt tttccaactg cgtaaggtgc tttcttccat ctgttttttc
    13441 gcctctaacg ggtttaacaa acagtaagaa aagtgatgat cttgaaagaa cttttcgact
    13501 ggtctggaat aaatccccgt tgcttcaaaa acaatcaatg gacgctcagg caagtgctgg
    13561 atctcttcga gtaatgcatg aaatcctctt tgagtgtgtt caatctcgtc ttcaaataag
    13621 caggtgtcgt ctttgtatag gacactgtag ctgtgtccca ttgaaacatc taatgcaaga
    13681 atgtaaggca tttgttttcc tcctttttaa gttttgaaag aactttactg cttcatttcg
    13741 attccaattt cctatacacg ggctcatggc cccaacatac gtaaacctga ttctaaaatg
    13801 acagtaaaga agttcggttt ttgataggga ctcgtagtcc tcaaggcttt gttcgaactt
    13861 tctttcactc ttcactataa tctaaaaata gaaaatagag gagaacatca ttcgtcaatg
    13921 atgttcccct ctaatcttag tatgtttttt atagtaattc cttcagatac tttcttgcct
    13981 catcattgaa acgcagtgat tcttctaact tctgataata ttccaacgct tgtttctgag
    14041 cttcagtaaa acgataatgt tttcctttcc atttgtcgat tgtctcttgc acatttccag
    14101 attggatatc tttcactact agttgccatt tttcttttga gactgtacgg tctttcttat
    14161 cagcagattg tatagcttct cgttctttac gcaatacttt taaaaaacca tttctgacta
    14221 tccgaggaat gctgtcgaag aacccttctg taaaaccata gcgacaatat tgcttccaaa
    14281 taaatagata gatttttggt tggatgaatc gttggttttt cgcaaaatat tccatctgag
    14341 cagtgacttc tggtgttcgt tgacgttttg tctttctcaa cacggaaata aatgtttcaa
    14401 aataagcatt aggatgtact ggcacttctt catatacgat ggattgccaa tctttacgta
    14461 aaaatgcgcg atagttgatc aagaattctt ctacatcctt tactaattct tctgcaaagt
    14521 cgtcaaatag ttcttttttt atctgtcgct tttttgactg cccattgatg tgttcactta
    14581 ttttcttgat ttcattttcc aatgcttgat aatccgctat gtaaatcggc atttctactt
    14641 ggcgaaattc tgcgagccgc tgagctaatt ttatatccgg taaatatggc tgtttcatcc
    14701 gacgattttg gtacaagaca aaacagtatt tttgccatag tccttcagga aaatcgatat
    14761 tcttctgctt aagccataat aattcgtcta aggccagatc aacttttgtc attccctgat
    14821 ggatcgaagc tgccactgtc ggagagacat gcaggtgatc ggcaaagtgg ttgatgccta
    14881 gttttaggat ctttccggtt tttggcgatt taacgatgta ttgcactttt aattctctac
    14941 cacactcaca atacaatttt tgctggcttc gttttgttcg aaaaaacgga ttgatcttga
    15001 agtcagaaaa cacccaatct gttgcagcta aataatgttc ttccataaaa cgagagctga
    15061 ttagatatcg gcggtgctta tcgactagtt cccgctgttt tttcgttaat tttgcataga
    15121 tctcccaacg ttgactgctt gtgtattgtt tagaaatttt gatgggatag cttttgctga
    15181 tttcttttgg aaaccattct tcaaagacgg attgccattc ttccacggct gtttctttcc
    15241 tttctaatta caaaaagctg gctactaaca taacgttagc aaccagccag tgtttcttgc
    15301 taaattagtg agttgttccg gcatttccgg aaatatcttc tttattttct actgctgctt
    15361 caactgcata agtcaaagaa gaagcaatgt cttctaaaga catagaaggc gtacccactg
    15421 gacgatcgat cacttgatcg ggtgagaatg gcacgtggat gaacccacct cgaattgccg
    15481 ggaattgtgt ttcgatcaaa tgaagcaaac gatacatgat atcattgcag acaaatgttc
    15541 ctgctgtata agagatgtac gctggtagac catgatcttg tacatttttt gtcattgctt
    15601 tgattggcaa tgaggtaaag taagcggctg gaccgttttc ttctaaagcc gtatcgaatg
    15661 gctgatttcc ttcattatca gggattcttg cttctgctag attgattgcg acacgttcga
    15721 acgagactgc tgaccgccca cctgcttgtc cgacacagat cacgacatct ggttggtgct
    15781 gttggatggc ttctttgact acttgactgc ttttttcgaa tacggtcggt acttccactt
    15841 tgatgatttc agcaccgctg atttcatctg gcattttttt tactgcttca taggctggat
    15901 tcactttgtc cccaccaaat ggatcaaaac ctgttactag tactttcatc tgttgttcct
    15961 ccgtccgatt gatttaacat atggttatct tttatcatac cattttcctt gaacagcgta
    16021 aataaaggaa acaatagttt aaataacata acagatcaaa cttaatatat tcttataaaa
    16081 aaatagtgtt ccactacaat gttagaagag gttgcgacaa ttattctgtc acaacccctc
    16141 tcttctatta tttaaataga atcatataag caatttggaa aactagcata aataacgcaa
    16201 taaacaactg gtttttgatc actccgtatt tgtctttcat ctccagcatc gctacaggca
    16261 cgatattgaa gttagcagcc atcggtgtca gcagggtccc acaaaaaccg caagtcaatg
    16321 caaccattcc tattaacgct ggattcgctc catgactaaa tacgaatggt gcaccgatcc
    16381 caaccgtcat cacggtgatg gccgcaaatg cattccccat gattaccgta aatatcacca
    16441 tgccaagagc gtaaatgatg atgccgatgt tcacatttcc ttctggaacg atcgtaccta
    16501 ctgcattgga aatcactgtc ccaactccag cgcttgtaaa taccgcacct agagaagcta
    16561 aaagcattgg caacatgctt aacggcccta ctgtgcccag catatctgct gcatcatcta
    16621 aaaatgtact tggtcgatta tctcgagaaa agaacatcaa gattagcatg gctgcaaaaa
    16681 caccgacgcc cacgccgact aaagcaccta aattggtaaa tagagcaaac aagatcgcaa
    16741 agacaccgat actcaaagct ggaataaaaa ttttcattcc tagtttgtct gacatttttt
    16801 ccatatattc ttttgatgga agccggcttt ctcctttgct gactttctta gcaattgctg
    16861 gtatggtcat cgcaaagatc aagccaccgt tcacgattgc cggaagccat cttcctcctg
    16921 caataactac ccctaagacc gtccagaaaa aggctgaccc ataacgatga ggattggttt
    16981 tatctgttaa atttcttact ccagtgtaaa tcaatacgag tcccatcacg atataaagga
    17041 tctccagtaa tttatcgcct aataaaatat caggatttgt aaaaaagtcc atcttattgc
    17101 ccctttcctg gataatattt cttcgctaat ttcttttctt taagatagaa gtagacactt
    17161 gccacaacta aagccgtgat agccactgga acttccacta aagcaagctg tcccaatgtt
    17221 acgtcatatc ctagatcttt cagtgttgat tggactagca acgcccctgc tcctccgatg
    17281 aatagtactt ggccaaagaa ccagcagaca ttttccatag ctgaagccat accttttagt
    17341 tcttcttcat gcttaggatg gacttctaac ccttttgatt cgatggtccc caatgccatc
    17401 ggtaaaatga ttggacgaac aaatccagca actccaccaa aactcacgtt aaaagccgca
    17461 aagatcattc gaaagatccc ataaatatct acaatcatac cagcagatac tttcttgaat
    17521 cgcttgatca aggaagccgc tgattctttc agaccatttc tttcaagcgt tcctgttgcc
    17581 aacataatga tgataaaaat cgccatcccg cgattgttta caaaacttgt acctaaaagt
    17641 tctaacattc cttgaatgcc aagaccgcca gtcaaagctg taacaactaa agcaaccatt
    17701 acgatcaata tcgagtctaa tttaaatgca aacccgagaa taacaatcaa gacgcctaaa
    17761 aggctaagat aattttccat atattttcct ccataaaatg acaatatttt tttaat
//
LOCUS       DAZKLA010000051        17146 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000051 DAZKLA010000000
VERSION     DAZKLA010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.051"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            160..468
                     /gene="rpsJ"
                     /locus_tag="ACG85N_001487"
     CDS             160..468
                     /gene="rpsJ"
                     /locus_tag="ACG85N_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFQ0224261.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            499..1128
                     /gene="rplC"
                     /locus_tag="ACG85N_001488"
     CDS             499..1128
                     /gene="rplC"
                     /locus_tag="ACG85N_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFQ0224262.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            1156..1779
                     /gene="rplD"
                     /locus_tag="ACG85N_001489"
     CDS             1156..1779
                     /gene="rplD"
                     /locus_tag="ACG85N_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFQ0224263.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            1779..2069
                     /gene="rplW"
                     /locus_tag="ACG85N_001490"
     CDS             1779..2069
                     /gene="rplW"
                     /locus_tag="ACG85N_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFQ0224264.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            2108..2941
                     /gene="rplB"
                     /locus_tag="ACG85N_001491"
     CDS             2108..2941
                     /gene="rplB"
                     /locus_tag="ACG85N_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFQ0224265.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            2984..3262
                     /gene="rpsS"
                     /locus_tag="ACG85N_001492"
     CDS             2984..3262
                     /gene="rpsS"
                     /locus_tag="ACG85N_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFQ0224266.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            3285..3632
                     /gene="rplV"
                     /locus_tag="ACG85N_001493"
     CDS             3285..3632
                     /gene="rplV"
                     /locus_tag="ACG85N_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFQ0224267.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            3646..4302
                     /gene="rpsC"
                     /locus_tag="ACG85N_001494"
     CDS             3646..4302
                     /gene="rpsC"
                     /locus_tag="ACG85N_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFQ0224268.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            4305..4739
                     /gene="rplP"
                     /locus_tag="ACG85N_001495"
     CDS             4305..4739
                     /gene="rplP"
                     /locus_tag="ACG85N_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFQ0224269.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            4729..4917
                     /gene="rpmC"
                     /locus_tag="ACG85N_001496"
     CDS             4729..4917
                     /gene="rpmC"
                     /locus_tag="ACG85N_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFQ0224270.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            4942..5208
                     /gene="rpsQ"
                     /locus_tag="ACG85N_001497"
     CDS             4942..5208
                     /gene="rpsQ"
                     /locus_tag="ACG85N_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFQ0224271.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            5266..5634
                     /gene="rplN"
                     /locus_tag="ACG85N_001498"
     CDS             5266..5634
                     /gene="rplN"
                     /locus_tag="ACG85N_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFQ0224272.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            5671..5979
                     /gene="rplX"
                     /locus_tag="ACG85N_001499"
     CDS             5671..5979
                     /gene="rplX"
                     /locus_tag="ACG85N_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFQ0224273.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            6007..6546
                     /gene="rplE"
                     /locus_tag="ACG85N_001500"
     CDS             6007..6546
                     /gene="rplE"
                     /locus_tag="ACG85N_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFQ0224274.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            6565..6750
                     /locus_tag="ACG85N_001501"
     CDS             6565..6750
                     /locus_tag="ACG85N_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFQ0224275.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            6786..7184
                     /gene="rpsH"
                     /locus_tag="ACG85N_001502"
     CDS             6786..7184
                     /gene="rpsH"
                     /locus_tag="ACG85N_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFQ0224276.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            7216..7752
                     /gene="rplF"
                     /locus_tag="ACG85N_001503"
     CDS             7216..7752
                     /gene="rplF"
                     /locus_tag="ACG85N_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFQ0224277.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            7913..8269
                     /gene="rplR"
                     /locus_tag="ACG85N_001504"
     CDS             7913..8269
                     /gene="rplR"
                     /locus_tag="ACG85N_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFQ0224278.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            8290..8790
                     /gene="rpsE"
                     /locus_tag="ACG85N_001505"
     CDS             8290..8790
                     /gene="rpsE"
                     /locus_tag="ACG85N_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFQ0224279.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            8805..8984
                     /gene="rpmD"
                     /locus_tag="ACG85N_001506"
     CDS             8805..8984
                     /gene="rpmD"
                     /locus_tag="ACG85N_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFQ0224280.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            9028..9468
                     /gene="rplO"
                     /locus_tag="ACG85N_001507"
     CDS             9028..9468
                     /gene="rplO"
                     /locus_tag="ACG85N_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFQ0224281.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            9468..10763
                     /gene="secY"
                     /locus_tag="ACG85N_001508"
     CDS             9468..10763
                     /gene="secY"
                     /locus_tag="ACG85N_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFQ0224282.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            10825..11472
                     /locus_tag="ACG85N_001509"
     CDS             10825..11472
                     /locus_tag="ACG85N_001509"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFQ0224283.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            11663..11881
                     /gene="infA"
                     /locus_tag="ACG85N_001510"
     CDS             11663..11881
                     /gene="infA"
                     /locus_tag="ACG85N_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFQ0224284.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            11914..12030
                     /gene="rpmJ"
                     /locus_tag="ACG85N_001511"
     CDS             11914..12030
                     /gene="rpmJ"
                     /locus_tag="ACG85N_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFQ0224285.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            12048..12413
                     /gene="rpsM"
                     /locus_tag="ACG85N_001512"
     CDS             12048..12413
                     /gene="rpsM"
                     /locus_tag="ACG85N_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFQ0224286.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            12441..12830
                     /gene="rpsK"
                     /locus_tag="ACG85N_001513"
     CDS             12441..12830
                     /gene="rpsK"
                     /locus_tag="ACG85N_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFQ0224287.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            12910..13848
                     /locus_tag="ACG85N_001514"
     CDS             12910..13848
                     /locus_tag="ACG85N_001514"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFQ0224288.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            13876..14256
                     /gene="rplQ"
                     /locus_tag="ACG85N_001515"
     CDS             13876..14256
                     /gene="rplQ"
                     /locus_tag="ACG85N_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFQ0224289.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            14528..15367
                     /locus_tag="ACG85N_001516"
     CDS             14528..15367
                     /locus_tag="ACG85N_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0224290.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            15343..16212
                     /locus_tag="ACG85N_001517"
     CDS             15343..16212
                     /locus_tag="ACG85N_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0224291.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            16202..16999
                     /locus_tag="ACG85N_001518"
     CDS             16202..16999
                     /locus_tag="ACG85N_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0224292.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT         5703 a         3112 c         3775 g         4556 t
ORIGIN      
        1 gcgtttactt tagtgaattg cacagacgta atcagaatga tttcgtcggc taagaaacga
       61 gaggttgcga cacgcccgga cgctttgcca cgaacgagcg tgacggaaat tttcgtggag
      121 ctatgtctac ttttcaaaat aggcgaagga gggaacaaga tggcaaaaca aaaaattcgt
      181 atccgtttaa aagcgtatga acaccgtatt ttagatcaat cagcggataa aatcgtggaa
      241 actgcaaaaa gaactggagc tagcgtgtct ggtccaattc cattaccaac tgaacgcagt
      301 ctttacacaa tcatccgtgc gactcataaa tacaaagatt cacgcgaaca attcgaaatg
      361 cgtacacaca aacgtcttat cgacattgtg aacccaacac caaaaactgt tgatgctttg
      421 atgaagctag acttaccatc tggtgtaaac atcgaaatca aattataaaa ctaaaacaaa
      481 aataatggag gtgtactcat gaccaaagga atcttaggga aaaaagtggg aatgacacaa
      541 atcttcactg aatctggtga attaattcca gtaactgttg tagaagctac tccaaacgta
      601 gtactacaag tgaaaacaat ggaaacggat ggctacgaag ctattcaagt tggttaccaa
      661 gatatgcgtg aagttttatc aaacaaacct gcgaaaggtc atgttgcaaa agcaaacacg
      721 gctcctaagc gcttcattcg tgaattcaaa aatgttgagc taggagaata tgaagtagga
      781 aaagaaatta cagtagatgt tttccaagca ggagacatta ttgatgtaac aggtactacg
      841 aaaggtaaag gattccaagg ggttatcaaa cgccacggac aaagccgcgg accaatggct
      901 cacggttctc gttaccatcg tcgtcctggg tcaatgggtc cagttgcacc taaccgtgta
      961 tttaaaaaca aacgtcttgc tggccgtatg ggtggcaacc gcgtaactat ccaaaacttg
     1021 gaagttgtac gtgtcgatgc agaaagaaat gttatcttga tcaaaggtaa tgttcctgga
     1081 gctaaaaaat ctttaattac tatcaaatca gctgtgaaag ctaaataatt gtaggaagaa
     1141 aggaggaact aaggaatgcc gaatgtagca ttattcaaac aagatggaag ccaaaacggt
     1201 gaaatcacac ttaacgaaga aatcttcgga attgaaccta atgaaagtgt tgtttacgat
     1261 gcaatcgtaa tgcaacgtgc gtcattaaga caaggaacac acgcggttaa aaaccgtagc
     1321 gctgttcgcg gcggcggacg taaaccatgg cgtcaaaaag gaacaggtcg tgcacgtcaa
     1381 ggatctatcc gctcaccaca atggcgtgga ggtggcgtag tctttggacc aacaccacgt
     1441 tcttacagct acaaacttcc taaaaaagtt cgtcgtttag caatgaaatc tgtattgtca
     1501 gaaaaagttg ctgaaaacaa cttggtagca atcgaaggct tgaacttcga cgcaccaaaa
     1561 acaaaagaat tcaaacaagt tcttgctaac ttatctatcg actctaaagt attggttgtt
     1621 ctagaaaaag gtaacgactt tgctgcttta tcagcacgta acttaccaaa cgtttctgta
     1681 gtaacttctg ataatgtgag tgtgctagac gttgtatcaa atacaaaagt gttagcaaca
     1741 caaactgctc ttactcaaat tgaggaggtg cttgcataat gaacttacta gacgtaatca
     1801 aacgcccagt gatcactgaa aaatcaatgc ttgccatgga tgacaagaaa tacacttttg
     1861 aagtggacac tcgcgcaaac aaaactttag taaaacaagc ggtagaagct gcttttgacg
     1921 ttaaagtcaa aaacgtgaac atcgtgaacg tacgtgcgaa attcaaacgt atgggcaaat
     1981 atgcaggata tacaaaaaaa cgtcgcaaag cgatcgttac attgactgaa gattcaaaag
     2041 aaatccaatt attcgaagct gctgaataag cactaataga taactcatta aattaggagg
     2101 gaaaaacgtg gcgattaaaa agtacaaacc tacctcaaat ggtcgtcgta acatgacagg
     2161 ttctgatttt gctgaaatca caacatcaac acctgaaaaa tcattgttac aaccattgaa
     2221 aaaccatgct ggacgtaaca acaacggccg catcactgta cgtcatcaag gtggcggaca
     2281 caaacgccaa tatcgcgtga tcgacttcaa acgtaacaaa gataacgttg ttgcaactgt
     2341 tcaaacgatc gaatatgatc caaaccgttc agctaacatc gcgttagtac actatgaaga
     2401 tggagtgaaa gcttacatct tagcacctaa aggactagaa gtgggtatga aacttgtttc
     2461 aggtccagaa gcagatatca aagtcggaaa tgctcttcca ttagaaaaca ttccagttgg
     2521 tactgttatc cacaacattg aaatgaaacc tggtaaaggt ggacaattga tccgttctgc
     2581 tggtacaagt gctcaagtac ttggtaaaga aggcaaatac gtattgatcc gcttgaactc
     2641 tggtgaagtt cgtatgatct tagctgcatg ccgtgctaca atcggttctg taggtaacga
     2701 acaacacgaa ttaatcaaca tcggtaaagc tggacgctct cgttggatgc gtaaacgccc
     2761 aactgtacgt ggtagcgtaa tgaaccctaa tgatcaccca cacggtggtg gtgaaggtaa
     2821 agctcctatc ggacgtaaag caccagtttc tccttggggt caaccagctc ttggttacaa
     2881 aactcgtaac aaaaaagcta aatcagacaa acttattgtt cgtcgtcgca aaaataaata
     2941 attactttga ccatgtgtcg cacattttga gaggaggttc accatgggtc gtagtttaaa
     3001 gaaaggacct tttgtcgatg agcatttaat gaaaaaagtt gaagctcaag caggggttga
     3061 aaagaaaaaa gttatcaaaa cttggtcacg ccgttcaaca gttttcccaa gttttgttgg
     3121 atataccatc gcagtttatg atggacgtaa acatgtacca gtttacatcc aagaagacat
     3181 ggtaggacac aaattaggtg aatttgcacc tacaagaact tatcgtggac atgctgctga
     3241 cgataagaaa acgaaacgct aatttgaggg gaggataaat ccaaatggca gaacaaatca
     3301 catcagctaa agcaactgca aaaacagttc gcacttcacc tcgaaaagcc cgtttagtaa
     3361 ttgaccttat cagaggtaaa agtgtcgcag atgcaatttc aatcttgaaa ttcacaccaa
     3421 acaaatctgc tggaatcatt gaaaaagtat tgatgtcagc agttgcgaat gcagaaaata
     3481 actttgactt agatgttgaa aacttggtag taagcgaagc ttttgttaac gaaggaccta
     3541 caatgaaacg tttccgtcct cgtgcaaaag gatcagcttc tccaatcaac aaacgtacaa
     3601 gtcatatcac ggtagtcgta tcagaaaaat aaggagggat aatcagtggg tcaaaaagta
     3661 catccaattg gaatgcgtgt aggcatcatc cgcgactggg atgctaaatg gtatgctgaa
     3721 aaagagtatg ctgagttctt acacgaagat ttaagaatcc gtaaatttat ttcaactaaa
     3781 cttgctgatg ctgctgtatc aacagttgaa atcgaacgcg caacaggtcg cgtaaacatt
     3841 tcaattcaca cagctaaacc aggtatggtt attggtaaag gcggttctga agtcgaaagc
     3901 ctaagaaaag aattaaacaa attaactggt aaaagaatcc atatcaatat cgtggaaatc
     3961 aaaaaaccag atctagatgc aaaattagta ggtgaaggaa tcgcacgtca attagaaaac
     4021 cgtgttgctt tccgtcgcgc tcaaaaacaa gccatccaac gctcaatgcg tgctggcgct
     4081 caagggatca aaactcaagt atctggtcgt ctaaacggag cagatatcgc tcgttcagaa
     4141 ggatactcag aaggaactgt tcctcttcat actttacgtg cggacattga ttacgcatgg
     4201 gaagaagcag atacaacata cggaaaacta ggagttaaag tgtggatcta tcgtggagaa
     4261 attcttccaa ctaaaaaaaa cactgagaaa ggagggaaat aatcatgtta gtacctaaac
     4321 gtgtaaaaca ccgtcgtgaa ttccgcggaa aaatgcgcgg ggaagctaaa ggcggaaaag
     4381 aagtagcatt cggtgaatac ggtttgcaag ctgttgattc acattggatc acaaaccgcc
     4441 aaatcgaagc tgctcgtatc gcaatgactc gttacatgaa acgtggtggg aaagtatgga
     4501 ttaaaatttt ccctcacaaa tcttatactg ccaaagcaat tggggtacgt atgggttctg
     4561 gtaaaggggc acctgaagga tgggttgcac cagtaaaacg tggtaaaatc atgtttgaaa
     4621 tcgcaggcgt tcctgaagaa gtagctcgtg aagcgttacg tctagcttct cacaaattac
     4681 caatgaaaac taagatcgta aaacgtgagg aaatgggtgg tgaatcgaat gaaggttaaa
     4741 gaaatcagag aattaaccac tgccgaaatg cttgatcaag aaaaacaatt aaaagaagaa
     4801 ttgtttaatc ttagattcca attagcaaca ggtcaattag aaaacactgc acgtattaaa
     4861 gaagtacgta aatcgattgc acgcatcaaa actgtgttgc gtgaacaagc taagtaatta
     4921 tggaaggagg ccattaagcg tatgactgaa ggaagaaatc aacgcaaagt ttatcaaggt
     4981 cgcgtggttt cagacaaaat ggataaaaca attaccgttg ctgtcgaaac aaagaaaaac
     5041 caccctatct atggcaaacg catgaactat tctaagaaat acaaagtgca tgatgaaaac
     5101 aacacagcaa aagttggcga catcgtgaga attatggaaa ctcgtccatt atcagctaca
     5161 aaacgtttcc gtttgttgga agtagtcgaa gaagcagtta ttatctaata acacgagatt
     5221 ttcctatata gattgaatgc aaaatctgaa aggaggatac acaacgtgat ccaagcagaa
     5281 agtcgtttaa aagtcgctga taactcaggc gcacgtgaaa ttttgacgat caaagtcctt
     5341 ggtggatctg gtcgtaaaac tgctaacatc ggtgatgtga tcgttgctac ggttaaacaa
     5401 gcaacgccag gtggggttgt caaaaaaggt gacgtcgtta aagccgttat cgttcgaaca
     5461 aaatcaggag ctcgtcgtac agacggttca tatattaaat ttgatgaaaa tgctgcagtg
     5521 attattcgtg atgataaaag ccctcgtgga acacgtatct ttgggccagt tgcacgtgaa
     5581 ctacgtgaaa acaacttcat gaagatcgtt tcactagcac cagaagtttt ataatcttga
     5641 gacacttatc aaaggaggtg cgaaacagtt atgtttgtta aaaaaggcga taaagttaaa
     5701 gttatcactg gaaaagacaa aaacaaagaa ggcgttgtac tagcagcgtt cccgaaaaaa
     5761 gataaagtta ttgtcgaagg tgtaaatgtc gttaaaaaac accaaaaacc ttctcaagct
     5821 gctcctcaag gtggaattgt tgagatggaa gcgccaattc atgtttctaa cgtgatggtg
     5881 gttgactcta ctggtgtagc tggccgagtt ggctacaaag aagtagacgg caaaaaagtc
     5941 cgtgtttcta aaaaaaccgg tgaagtttta gataaataaa agtttaagga aggagggtct
     6001 tactgaatga accgcctaaa agaaaaatat cttaaagaag taactccatc attgatggaa
     6061 aaatttaact atagctctgt tatgcaaaca cctaaagttg aaaaaatcgt catcaacatg
     6121 ggtgttggtg atgcggtatc aaacgctaaa aacttagata aagcagttga agaattaact
     6181 ttgatcactg gtcaaaaacc aatgattaca aaagctaaaa aatcaatcgc tggattccgt
     6241 ttacgtgaag gaatgccaat tggtgcaaaa gttacattgc gcggagaaag aatgtacgaa
     6301 ttcttagaca aattggtttc tgtttcacta ccacgtgtac gtgacttcca tggtgtaagt
     6361 aaaaaagcct ttgacggtcg tggaaactat actttaggta ttaaagaaca attgatcttc
     6421 ccagaagtag attatgattt agtagataaa gtacgtggta tggacatcgt tattgtaaca
     6481 acagcaaact ctgatgaaga atctcgtgag ttgttgacac aattaggcat gccattccaa
     6541 aaataattaa aggaggcgaa ctacgtggct aaaaaatcaa tgattgctaa aaacaaacgt
     6601 cctgcaaaat tttcaacaca agcttatact cgttgcgaac gttgcggacg tccacattca
     6661 gtttatcgta agttccatct ttgccgtatt tgcttccgcg aacttgccta taaaggtcaa
     6721 attcccggcg tgaagaaagc tagctggtaa aaagtaaatt cgcataagaa ggaggtaaaa
     6781 gatcaatggt catgacagat ccaattgcag attttctaac tcgtatccgt aacgcaaaca
     6841 tggttaaaca tgagtcttta gaagtacctg catcaaaaat taaacgtgac atcgctgaaa
     6901 tcttgaaacg tgaaggtttc gttcgtgatg ttgaatacat cgaagatgac aaacaaggcg
     6961 tgatccgtgt tttcctaaaa tttggtaaaa acggcgaacg tgttatcaca aacttaaaac
     7021 gtatctctaa accaggtttg cgtgcttatg tcaaagctga cgaagtacca aaagtattga
     7081 acggtttagg tatcgcaatc atttcgactt ctgaaggtgt tgtcactgat aaagaagctc
     7141 gtgaaaaaaa tatcggcggc gaagttatcg cttacgtatg gtaatcaagt agaatctaca
     7201 aggaggtgtc tttaagtgag ccgtatcgga aataaagtcg ttgttatccc tgaaggagta
     7261 acagttactc aagacggaaa caacgttaca gttaaaggac caaaaggtga attgactcgt
     7321 actttttcag cagatatcaa aatgaatatc gaaggaaacg aagtaacttt cactcgtcca
     7381 aacgacagta aagaaatgaa aacaattcat ggaacaactc gtgcgaactt caacaacatg
     7441 gttgtaggtg tcagcgaagg tttccaaaaa gcgttagaac taatcggggt tgggtaccgt
     7501 gcacaattac aaggatcaaa acttgtattg aacgttggtt actcacatcc agtagaaatc
     7561 acaccaccag ctggagtaac tgttgaagta ccagctaaca cacaagtaat tgttaaaggt
     7621 gcgaacaaag aagaagttgg cgaattagct gcaaacattc gtggaactcg tcctccagaa
     7681 ccttataaag gtaaaggtat tcgttatgtt ggcgaatatg tacgccgtaa agaaggtaaa
     7741 actggtaaat aatcgtttag aaaaattgca tagaagccag cagcaggaaa aaggcatttg
     7801 gctgaagttt accttcaaaa aaatacattt ttacttgtgc cgcagcttct atttttcgaa
     7861 acgtatttac tgcttgaaat agtagtacaa taaaaataaa gaggtgacaa ttgtgattac
     7921 aaaaccagat aagaacaaaa cacgtcaaaa gagacatcgt cgtgtacgta acaatatctc
     7981 tggtactgct gagcgcccac gcttgaacgt ttttcgttct aacaaaaaca tctacgctca
     8041 aattattgat gacgtagcgg gtgtaacgct agcaagtgcc tctaccttgg ataaagaaat
     8101 ttcaggtgga acaaaaacag aagcagcaca agctgtcggt aaactagttg ctgaacgtgc
     8161 agctgaaaaa ggcattaaag tagtagtctt tgaccgtggt ggataccttt accatggccg
     8221 tgtgcaagct ttagctgaag ctgctcgcga aaatggacta gaattttaga agaaggagga
     8281 ataccattca tggtttatat cgatccaaaa catttggaat tagaagaccg cgttgttgca
     8341 attaaccgtg taacaaaagt tgttaaaggt ggacgtcgtc tacgttttgc tgctttagtt
     8401 gttgtcggtg acaaaaacgg acacgtagga tttggtacag gtaaagctca agaagttcct
     8461 gaagcaatcc gtaaagcaat tgaagatgcg aagaaaaact tagttgaagt acctatggtt
     8521 ggttcaacaa tcccacacga agtaatcggt gcttttagtg gtggtagaat cctaatgaaa
     8581 cctgctgtag aaggttctgg ggttgctgct ggtggaccag ttcgtgccgt attagaatta
     8641 gctggggtag cagatatcac ttctaaatct ttaggctcaa atacaccaat caacgttgtt
     8701 cgcgcaacag ttgaaggttt gaaacaattg aaacgtgcag aagaagttgc agaacttcgc
     8761 ggcaaatcag ttgaagaatt aatcggataa ggaggacaac aataatggct gaattaaaag
     8821 ttactttaaa acgcagtatt atcggacgtc ctcaaaacca acgcgataca gttaaagcgt
     8881 taggtcttac aaagattaac agttcagtgg ttaagccttc taatgaagca attaaaggca
     8941 tgatcaatac tgtttctcac ctagtggacg ttgaagaaat ttaatttaga aaaatcataa
     9001 ctagaacatt aaggaggtgc caaacctatg aaacttcatg aattaaaacc tgctgaaggt
     9061 tcacgccaag tacgtaaccg tgttggtcgt ggtacttcat ctggtaacgg aaaaactgcc
     9121 ggacgcggac aaaaaggaca aaaagctcgt tcaggcggcg gtgtgcgttt aggatttgaa
     9181 gggggacaaa caccattgtt ccgtcgttta ccaaaacgag gattcacaaa cgtgaaccgt
     9241 aaagactatg cagtcattaa tcttgatgta ttgaaccgct ttgaagacgg agctgaagta
     9301 actcctgttg ctttagttga agctggaatc gtgaaaaacg aaaaagctgg aatcaaagta
     9361 ttagctaatg gggaactaaa caagaaatta actgtgaaag cagcaaaatt ctctaaagca
     9421 gctcaagaag caattgaagc ggctggtgga tcaatcgagg tgatctaatg tttagactat
     9481 taaaagatgc ttttaaagta aaagacatta gatcgaaaat cctatttact gtatttgtgt
     9541 tgtttgtatt tcgcctaggt gcacatatta ctgtaccagg cgtaaatgct gcttcattgc
     9601 aaaacctttc ttctttgcca tttttcaaca tgttgaactt ggtaagtggt agtgcgatgc
     9661 aaaacttttc cattttctca atgggggttt caccatacat tactgcttct atcgtcattc
     9721 agcttcttca aatggacatc gtgcctcgat ttgttgaatg gtcgaaacaa ggggaagttg
     9781 gtcgtaaaaa actgaatcaa gcgactcgat atctgacatt agtattggca tttatccaat
     9841 ctgtcggtat cactgctgga ttcaatcaat acgctcaatt aggtttcgtg aataatccta
     9901 acgtagcaac ttacgtaacg atcggtttga ttctaacagc cggtacgatg tttgttactt
     9961 ggctaggaga acagatcaca gaaaaaggta tcggaaatgg cgtatcaatg atcatttttg
    10021 caggtatcat ttctagattg cctgaaagta tcagagaaat ttatgaagat tactttataa
    10081 atattgatcc ttcagacata tggaaatctg ctatttttgt ggccatttta ctagtagcgg
    10141 tcttgttgat cgtgacattc gtaacatttt tccaacaagc tgaacgcaag atcccgatcc
    10201 aatatacgaa acgcgtagct ggcgcgccta caagcagcta tctgccgttg aaagtaaatg
    10261 cagctggagt tattccagtt atctttgcta gttcgtttat tgctacgcca aatgctgttt
    10321 tacaagcatt gagcggtaga ttcggttctg aaaactggta caaagtgatg atggagatct
    10381 ttaactataa tacagcacct ggtgctacga tttatacgat tctgatcgtt gccttcactt
    10441 tcttctacgc ctttgttcaa gtaaaccctg agaaattagc ggaaaacttg caaaagcaag
    10501 gaagttatat accaagcgta cgccctggta aggggactga agagtatgtc tcaagattac
    10561 tgatgcgttt aagtacagtt ggtgcagtct tccttggttt agtggcattg cttccaatca
    10621 ttgctcaaat ggtttgggat cttccacaat caatcggatt aggcggtaca agcttactga
    10681 tcgttatcgg tgtagcttta gaaactgcga aacagttaga aggcttaatg ctgaaacgta
    10741 aatatgttgg attcattgag taagaaagcg tgttgaggtt tcccctcaac acacgttctc
    10801 acacatttat taggagggaa aacaatgaac ctcattttaa tggggctgcc aggcgcaggt
    10861 aaaggaacac aagctgaaaa aatcattgat gcttatggta ttccgcacat ctcaacagga
    10921 gatatgttcc gcgcagcgat gcaaaacgaa acagctcttg gcctggaagc aaagtcttat
    10981 atggataaag gtgcattagt tcctgatgaa gtaacaaatg gaatcgtgaa agagcgacta
    11041 gctgaaccag atacagagaa aggcttctta ttagatggct ttccacgtac gctagatcaa
    11101 gcaaaagctc tggacgcaat gctaaaagat ttgaataaaa aaattgatgc tgtcatcgat
    11161 atccatgttg gtgaagaaat cttggtcgaa cgtctagccg gccgatttat ctgcagcaat
    11221 tgtggagcaa cttaccataa aattttcaac cctacaaaag ttgaagacac atgtgatcgt
    11281 tgcggtgggc atgaattcta tcaaagagaa gatgataaac ctgagacggt taaaaatcgt
    11341 ctggctatca acatcaaaaa tagtgaacca atcttggctt attataaaga acaaggtttg
    11401 ctaaacacta ttgatggtgc tcgtgaaatc gatgctgtat ttgcagatgt acaaaaaatc
    11461 attgaaaaat agcacgattg cccgtagccg acttgcatta tgcagttact cttgtcaagt
    11521 tcaataaatt atgatagaat atgtcagtcc gacttctaga gaatcctcta gatgatccat
    11581 tttgagaatt ttgaggtggg aactgcagtt tccgccattt tatcgtgtga aaatgcgaaa
    11641 caagtacaag gaggtactgt gcgtggcaaa agaagatatg attgaagtcg aaggtacagt
    11701 cgtcgaaact ttgccgaatg caatgtttaa agttgaacta gaaaacggac accaagttct
    11761 tgctactgtt tcaggtaaaa ttcgtatgca ttacatccgt atcttacctg gtgacaaagt
    11821 aacggttgaa ttgtctccat acgatcttaa ccgtggccgt attacttatc gctttaaata
    11881 attgtactcc gtaaaactac aggaggtatt atcatgaaag taagaccatc agtaaaacca
    11941 atgtgcgaac attgtaaagt aattcgtcgt aaaggacgcg ttatggtgat ttgcccagca
    12001 aatccaaaac ataaacaacg tcaaggataa ttggaggtgc tataaatatg gctcgtattg
    12061 caggagtaga tattcctcgt gataaacgtg tagtagtttc acttacttat atctatggta
    12121 ttggtaacac tactgctaaa aagatcttag ctgatgctgg tgtatctgaa gatgttcgcg
    12181 ttcgtgaatt aacgaatgaa caaacagatg ctatccgtgc agaagtagat aaattaaaag
    12241 ttgaagggga tcttcgtcgt gaagtgaact tgaacatcaa acgtttgatg gaaatcggtt
    12301 cttaccgtgg aatccgtcat cgccgtggat tgcctgttcg tggacaaaac acgaaaaaca
    12361 atgcacgtac tcgtaaaggc ccagctcgcg ctatcgctgg caagaaaaaa taattcttaa
    12421 gtgaaggagg ttaaaacttc atggcagcaa aaaaagtgaa tcgtaaacgc cgtgtgaaga
    12481 agaatataga agcaggtatt gcacatatcc attctacatt caataacact atcatcatga
    12541 tcactgatac tcacggaaat gcattagcgt ggtcatcagc tggttcatta ggtttcaaag
    12601 gaagcaaaaa atcaactcct tttgccgctc aaatggcagc agaaactgct acaaaagcag
    12661 caatggaaca cggattaaag actgttgaag taacagttaa aggacctggt tctggacgtg
    12721 aagcagcaat tcgttcatta caagctacag gtttagaagt gactgcaatt cgtgacgtga
    12781 ctccagttcc tcataatggt gcccgccctc caaaacgccg tcgtgtttaa tgagtgccgc
    12841 tcattttgag ttttatcagt aacgtcattg aacaagacac ttttcgtttt gaaaggggta
    12901 aagataagaa tgattgagtt cgaaaaacca agaatcgcaa aaattgatga agaaaaagat
    12961 tatggcaagt tcatcgttga acctcttgaa agagggtatg ggactacttt agggaattcc
    13021 ctacgtcgta ttttattatc ttctttgcca ggtgctgcca tcactaatat tcaaatcgat
    13081 ggtgtactgc atgaattctc taccatcaaa ggtgttagag aagacgtcac tcaaatcatc
    13141 ttgaacatta aaggcttagc gctgaaaatg tatggccaag aagaaaaaac ccttgaaatc
    13201 gatattaccg gacctgctac agtaactgct ggcgatatta tcgttgatag tgatgtagaa
    13261 attcttaaca aagatatgta catctgtagt gtctctgaag gagctacgtt ccatgctcgc
    13321 ttaacagtga aacctggacg tggttatgtt caagcagatg aaaataaaaa agaagatatg
    13381 ccaattggcg ttcttccagt cgattcaatc tacacacctg ttcgtcgtgt gaactatcaa
    13441 gttgagaaca cacgtgttgg acaccgtgaa gattttgata aattgacgat ggagatttgg
    13501 acagatggtt caattgaacc gttggaagca atgagtttgt ctgcgaaaat catgactgaa
    13561 catttggata tttttgtgaa cttaactgat gaagcaaaaa atgctgaaat catggtcgaa
    13621 aaagaagaaa cacaaaaaga aaaaatgttg gaaatgacga tcgaagagtt agacttgtct
    13681 gttcgctctt acaattgttt gaaacgtgca ggaatcaata ctgtacaaga acttacaaat
    13741 aaatctgagc ctgaaatgat caaagtccgt aacttaggac gtaaatcatt agaagaagtt
    13801 aaagcaaaat tgcacgactt aggtttaggt ttacgtaaag aagattaaaa ccttttacga
    13861 aggagggaaa ccaacgtgag ttatcgtaaa ctaggacgca catctagcca acgtaaagcg
    13921 atgttgcgtg atttaacaac tgatttatta atcaacgaac gtattgtgac tactgaagct
    13981 cgtgcgaaag aagttcgctc aactgcagaa aaaatgatca ctttaggtaa acgcggagat
    14041 ttgcatgcac gtcgtcaagc tgcggctttc gtacgtaatg aagttgccag cgtgcgtgaa
    14101 gaaaatgagg acatcgtcat tgaatcagct ttacaaaaac tgttcaacga tattgcacct
    14161 cgttacgcag aacgtcaagg cggatacaca cgcattttga agacagaacc aagacgcggt
    14221 gatggtgcac caatggttgt cttagaactt gtctaaaatc acactaataa catcacttta
    14281 tagatgattc ttttagagtg ttatgatgtt ggaagatatt tttccgagtc tagctcagcg
    14341 ctgctcctca agcgaatatc gcttggggag taagcactca tcataaagtg tttttttata
    14401 ccttcaatta cctgtcatga cagatatgtt tgaaggcata aaaaaacatt ttaagcgaga
    14461 aataattgaa aattttgtct ttttttgata gtgttagtag gaactttagg atgaagaagg
    14521 agtaatcatg gagccaatta ttgaattaga aaagataaat tataaatacc agccagatga
    14581 tcttcgcccc gcattgaaag atgtctcctt tacaattgat aaaggtgagt ggatcgctat
    14641 tattggacac aatggttcag gaaaatcgac acttgccaaa acgatcaatg gattgcttct
    14701 gccagaatca gggatcgtga aagtgggaaa tcaaatattg gatgaagaaa atatttggac
    14761 gatccgacag atggttggaa tggtttttca aaatccagac aatcagtttg ttggttcgac
    14821 agtagaagat gacgttgctt ttggtcttga aaatcaaggg attcccagag aagaaatgct
    14881 agtgcgtgtg aaagatgcac ttgagaaagt tagaatggct gagtttgctt cacgcgagcc
    14941 tgctcgttta tcaggagggc aaaaacaacg tgttgccatt gccggcgtgg ttgccttacg
    15001 acctgatatc attattttag atgaagcaac cagtatgcta gatccagaag gtcgtgaaga
    15061 agtgatctct acgatcaaga agatcaaaga agaaagtcaa ttgaccgtta tatctatcac
    15121 tcatgatatt gatgaagcag ctaatgccaa tcggatacta gtgatgagac aaggcgaact
    15181 tgtccgtgaa ggtacaccaa aagaaatttt ttctgctggt cctgaactga tcgacttagg
    15241 tctagatcta ccatttcctg aaaaactgaa gagcgcactg aaagaacgtg gggttgatgt
    15301 gcccagtgaa tatatgacag aagaaaggat ggtggattgg ttatggacat ccgttttgaa
    15361 caagtagact tcacttatca gccgaataca ccatttgaac aaagagcctt atttgacatc
    15421 aatatgacga tcaaagaaaa cagttatact gcattagtag ggcataccgg aagcgggaaa
    15481 tcaaccttac ttcagcattt gaatgcgctt gttaagccaa caagcggtac agtccatatt
    15541 ggagaacgag atattcagcc ggatacggat aataagaatt taaagcccat ccgaaaaaaa
    15601 gttggcatcg tctttcagtt tccagaagca cagctattcg aagaaacggt agcaaaagac
    15661 attgctttcg gtcctaaaaa ctttggtgtc agcgaagaag aagcactagt cctagcaaaa
    15721 gaaacattag aacaagttgg gctggatgaa agctatttgg aacgttcgcc atttgaactt
    15781 tcaggagggc aaatgcgtag agtagcaatc gctggtgtgc ttgccatgag gccggaagta
    15841 cttgtattgg atgaaccaac ggcaggactt gatccccagg gcagaaaaga aatgatggag
    15901 atgttttggc gtcttcataa ggaacagcag atcactattg tattagttac gcatttaatg
    15961 gatgatgtag ccaattttgc agattatgtc tatgtgttgg aaaagggacg aatcgttaat
    16021 agcggggagc cgcaagaggt ctttcaaaac attgaatggc taaaagaaaa acaactaggc
    16081 gtacctactg ccaccgagtt tgcagaagaa ttaatggcta aagggatgaa ctttgctacc
    16141 ttgcctttga cagctgaaga attagcagac gctatcgtcc attatgctgg aggaaatgcc
    16201 catgatgaat aaattgatat ttggtcgtta tatgcctgga gattccttga tccatcgatt
    16261 ggatccccga gctaaattat tagccagttt ttattttatc ggaatcattt ttttagcaaa
    16321 taattgggaa acgtaccttt tgttggcatt tttcacatta ctttctgtct tcttgtcaaa
    16381 agtagatttg aaatttttta tccgaggcat tcgcccattg atctggctaa tcctcttcac
    16441 tgtagctttg caggtcttgt ttacaagtgg cggagaaact tattggagtt ggggtgtttt
    16501 ccatatcact gaatttggtg tgatgaacgg cctctttatt ttctgtcgat tcgtgttgat
    16561 catttttatg tcgactctac tcacgcttac tacaccgcct ttggaattat cggatgcgat
    16621 tgagtacttg cttcgtccat tgaaagccgt acggttccca gtccacgaaa tttcattgat
    16681 gctgtcgatt gctcttcgtt ttgtgcctac attgatggac gaaacagaaa agatcatgaa
    16741 cgctcagcgc gctcgcggtg ttgattttgg tgaaggaaac ctagttcaga aaatgaaagc
    16801 agtcattcct ttgctgattc ctttatttgt cagcagcttc aaccgtgcgg aagatcttgc
    16861 gactgctatg gaagcaagag gctacaaagg tggggaagga agaacgaaat atcgtgtatt
    16921 gcattggcat aatagagaca cgatggttat cattgctttt gtattattaa ctgtgctact
    16981 gatcttttta agaggataaa cgttcagtgg gccgctatct attcttttct tgctgatctg
    17041 tttgcaagaa aggatgcaag cagtgtatga caagaaatat tagggtctgt ggcagactaa
    17101 ggtcacaggc ctctttcttt tatctgaaaa gaaagaacga aaaaat
//
LOCUS       DAZKLA010000052        17089 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000052 DAZKLA010000000
VERSION     DAZKLA010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.052"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(458..1087)
                     /gene="udk"
                     /locus_tag="ACG85N_001519"
     CDS             complement(458..1087)
                     /gene="udk"
                     /locus_tag="ACG85N_001519"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFQ0224293.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1171..1866)
                     /locus_tag="ACG85N_001520"
     CDS             complement(1171..1866)
                     /locus_tag="ACG85N_001520"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFQ0224294.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1879..2595)
                     /locus_tag="ACG85N_001521"
     CDS             complement(1879..2595)
                     /locus_tag="ACG85N_001521"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFQ0224295.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2925..3533
                     /locus_tag="ACG85N_001522"
     CDS             2925..3533
                     /locus_tag="ACG85N_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFQ0224296.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="ACG85N_001523"
     CDS             3858..4181
                     /locus_tag="ACG85N_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFQ0224297.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="ACG85N_001524"
     CDS             4363..5733
                     /locus_tag="ACG85N_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFQ0224298.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="ACG85N_001525"
     CDS             complement(5863..7164)
                     /locus_tag="ACG85N_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0224299.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7518..9509)
                     /locus_tag="ACG85N_001526"
     CDS             complement(7518..9509)
                     /locus_tag="ACG85N_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFQ0224300.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9653..9919)
                     /locus_tag="ACG85N_001527"
     CDS             complement(9653..9919)
                     /locus_tag="ACG85N_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0224301.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10429..12555)
                     /gene="pnp"
                     /locus_tag="ACG85N_001528"
     CDS             complement(10429..12555)
                     /gene="pnp"
                     /locus_tag="ACG85N_001528"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFQ0224302.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12743..13012)
                     /gene="rpsO"
                     /locus_tag="ACG85N_001529"
     CDS             complement(12743..13012)
                     /gene="rpsO"
                     /locus_tag="ACG85N_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFQ0224303.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13263..13826
                     /gene="def"
                     /locus_tag="ACG85N_001530"
     CDS             13263..13826
                     /gene="def"
                     /locus_tag="ACG85N_001530"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0224304.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14079..14600)
                     /locus_tag="ACG85N_001531"
     CDS             complement(14079..14600)
                     /locus_tag="ACG85N_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFQ0224305.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14603..15445)
                     /locus_tag="ACG85N_001532"
     CDS             complement(14603..15445)
                     /locus_tag="ACG85N_001532"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFQ0224306.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15438..16163)
                     /locus_tag="ACG85N_001533"
     CDS             complement(15438..16163)
                     /locus_tag="ACG85N_001533"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFQ0224307.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16339..16635
                     /locus_tag="ACG85N_001534"
     CDS             16339..16635
                     /locus_tag="ACG85N_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224308.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16891..17065
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
BASE COUNT         4819 a         3541 c         3172 g         5557 t
ORIGIN      
        1 ggggtgcgac aaaagagctt cttcaaaaaa ataagtcgaa atctcacaca aattcgagaa
       61 ataattttcg taagtttctt cttatttatt gcatctgacc acttctgtta caacctctcg
      121 ttaaacggtg ttcaaaagct tgcatctacg ataataagcc caacccaacg aaaaacgtga
      181 ggagctgttt tatttggatg ctcttatcgc ttgatgcaaa gacagctttt ttacaacctc
      241 tacttcacgg tgctcaatca gctgaccttc ggtattagct caaaaaacgg taaaatataa
      301 acagctattt tccgtttctt ctttttaata cttaggtcaa gatgctgttt tcacaacctc
      361 ttgttataaa taaaaaaaca gttggctggt gactgttcaa aaagcagaat ccttgctttt
      421 caaacggtcg cagtccgttc aactgtttcg ctgcgcacta tttatggttt aagaaactag
      481 ctacttttgt tgtaatcaaa tcaatagcaa cgtggttttc tccgccttct ggaacaatga
      541 tatccgcata gcgcttcgtt ggttcgataa attgatgata catcggttta acaactgtca
      601 aatattgttc gatcacggag tccaatgtgc gtccccgttc ttccatatcc cgtttgatac
      661 gacgaatgat acgaatgtca tcatctgtat ctacgtatac tttgatatcc atcaagtcac
      721 gcagacgttc gtcttctaaa atcaggattc cttctagaat gatcacttct tttggttcct
      781 gaatgatcgt tgcctgactt cttgtatgcg ctacatagtc atacacgggt ttttcaatgg
      841 ctttatagtt cagtaagtcg cctacatgct gaatcagcag atcattatca aaggcaaatg
      901 ggtgatcgta atttgtgttc aatcgttctt caaaactcaa atggctctga tccttgtaat
      961 aagaatcttg ttcaagcatc atgatggaat ggtccgggaa gttgttgaaa atcgcacggc
     1021 tgacacttgt ctttccgctt ccggatccac ccgtcacacc aatgataatt ggcttgcttt
     1081 ttgtcattga ctaaaacctc tttcgtatcc gttttttcat tctaatctat tatagcgaaa
     1141 aaacacgaaa atgctatatt tttctgaaaa ttaatccttt tcaatttcca ataatgaata
     1201 aagttcctct aatgaatcga ttcgataagt aggctggatt tcttcgattt ctggtaattt
     1261 atttctattg aaccaaatcg tatcgatccc tgccatattt cctcctttga tatcagaagt
     1321 caaagaatca ccaatgatca cagtttgctt tttatcaaaa ttaggaatgt gggaaaagac
     1381 atgatcaaaa aattctttca tcggcttttg cgctccgaca gcttctgaga caaagatatc
     1441 cttgaagtaa tcaagcattc ctgcatctgt taatcgccga tgttgcgtgt gcgacacacc
     1501 gttcgtcaca atataaagat cagcatgcgg atggaccttt ttcaggattt ctagacttcc
     1561 tggaacaaga tcatgacctt ggttcaagta gtggcggtag cgtttttcca tttgatcact
     1621 atctactgtt ttcccaaatt gctggaaaag taacccaaaa cgactgccta ttatttcttc
     1681 tcgtgatagt ctgccttttt caaactcctg ccataaatga tgattcaaag ccttatagct
     1741 ggtttcgatt tctggtgtca atgtgattcc ttgttctgca aacaaagcac ttaaagcatt
     1801 ggcttcagtt aattgaaaat ctaacaatgt atcatctaca tcaaacaata aagttttata
     1861 tttcatcttt ttgacctcct atttaaaata aatacttaac tttttcatct ctgctaatgt
     1921 caacgatcga tactcgccta ctcctagctg ttcatctaat tccaatggtc ccatgcttag
     1981 ccgtttcaat gcggtcactt tctttccttg ggctaagaac atctttttga cttgatggaa
     2041 ttttccttct tggatcgtca ataaaaccgt actttggtgc ttgtctgctt tgagtatttt
     2101 taaatcagcc ggcttacatt gatacccatc agcgaatacg attccatttt caaatgctgt
     2161 tacgtctgcc ggtgtcaccc tttcgttcac aactacttga taagtctttg aaaccttttt
     2221 ttctggaagt agcatttcat atcccaattg tccattcgaa gtgatcagca atagtcctgt
     2281 tgtatctcta tctagtcttc ccactggata taatggttgg aaacgatcgg cacttgcaat
     2341 cagatcgatt actgtcgtct tttctggatc tgatacagct gtaacagctt tttcaggttt
     2401 attgagaaga taataggact catttgtttc taagtgttca cctgctactt cgatttgatg
     2461 cagtaaacta tctacatttc gattttgtcg aaattcgact gtgccatcta tcatcacttg
     2521 cttcaaagca aacagccgct tcatttcctt gcgtgtcgtt tctaattttt cttctattaa
     2581 tttatctaaa cgcatgctgt ttcctttctt attactacta attaattata ccttgaatat
     2641 tcttgcttgc aaatacatca tcatagttat tccttgactt aattaacagt tcacgttacg
     2701 gttaaatagt aaagtgaatt ggatttctca tacacagcgt aatgacctgg tggagaaaag
     2761 ttgaatcctg actttctaag gggagatgcg cgcatctctc cttttgcgct tttttaagcg
     2821 ccctgccttc gatgatgtgc acgagattag cctttagaat tcacttaaat aaaatctgtc
     2881 cgttaccaaa gcagacagtc acattttaag gaggaatttt ggttatgtca gtatcattag
     2941 aagtaagtaa aagagaagta cgtccccgtt cattaagaaa caaacttcgt cacgaaggga
     3001 aagttccagc tgttgtttat gggtataaag tagaaagtac ccctatctca ttcgatggtt
     3061 tgacactaag caaattgttg cgtgaacatg gagcgaacac agtcatcacg atggacatcg
     3121 atggacaaaa agtcaataca ttggttcaca agatccaaac gaacactttt accaatcatt
     3181 tcgaacatgc agaatttcta tctgtaaaca tgtcggaaga aacagaagtc gaaacagaag
     3241 tcgttcttgt tggcgaatca gtgggtgcta aatctggtgg agtgttagct caaaacttgt
     3301 atacagtagt cgtatctgct acaccagaca aattgcctga acgaattgaa gttgacgtga
     3361 caaacttgga attaggtgat tctattacag tagccgatct tccaaaaaac gacgattaca
     3421 aattcgtgac cgacagtgaa gagcaaatcg ctgctgtagt cgctcaaagt gaagaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatcttgc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattctg tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 cttacccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat aatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttatcacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aaaacggtat tcaaaagctt gcatctacgg cattaagccc
     7381 aaacagatga aaaacatgag aagctgtttt tatctgctcg ttcttactgc ttgttgcagt
     7441 acagcttttt catgacctct gataaacggt gttcaagtag ctgacattcg acattagttc
     7501 aggtcaaagc gctactttca caaccgctct ttcaaccatg ttttccgagt atttccaatg
     7561 ataagcggaa cagtttcatg ttttacttcg ctgtactcta gcccaaacca tgtttctggc
     7621 gaggtagcga tttttttgat tgccagtttt gcctgcatga tttgacgttg ccattttcct
     7681 agagttgtca cattggaaac tacttcatca atcaggacaa attggaaatc gccaacttct
     7741 cgccctggtg tcagagagta tctttgtggc tgttttggca aacggccttg tttcatcaaa
     7801 tcatggatga cttggcgaac atatacgttg atttcctgtt ggaccctgaa acctaagaac
     7861 agattcactt ggacaataaa atcagtgtcc atcatatcta cttgatattc tttcgtatat
     7921 ggctcatccg tcacttccac atgaacaaac caatagactc gtgctctttt aggccgttta
     7981 tctaaaatcg aatagatgaa ctgtcggcca actttatctt cagccatatc aggaaccaag
     8041 aagaccacat ttgtctgata gattggtaaa gaagtatcat cacgtaattc tctaagctgc
     8101 gggatataat ctctcaaaga cacatcctcg gttgacgatt cgcgaataat gttcccttgt
     8161 tcccaaataa acatcacgac taagatgatc aaaccaatca gaacggctac aaaaccgcct
     8221 cggaagaatt tgacaacact tgagataaag aaaacgactt ctatcgcacc gaagaaaaag
     8281 gtaactaaat atgcaagcca acgagaatgc ccattttgga taagataaaa gtaaagcagt
     8341 gctgttgtca tcaacatcgt cacagtgatc gatagaccgt aagcagcttc catatgatga
     8401 gacgttcgga atgtgacaac cactagagaa caggcgatcc ataaaatcag attgactgct
     8461 gggatataca tttgcccgat actgttccca ggatacatga tctttaaccg aggcagcagt
     8521 ttcaacttga ttgcttctga aactagcgta aacgaacctg taatcaatgc ttgcgaagca
     8581 atgactgcag cgactgttgc aaaagcaacg ccgatcacga tccaactttg aggcatcatt
     8641 tggaagaatg gattcaggtt cttgatttgc tgtgcttcag ggttttgata aacattcaac
     8701 aaccacgcag cctgcccaaa ataattgata accaaacaaa ttttgacgta aggccagctc
     8761 agacgaatat ttcgtttgcc gacatgtcca agatcggaat aaagcgcctc tgcacctgtc
     8821 gtagccagga aaatatttcc aagaatgaac aagcctaatt tgttatctgc actaaaaagc
     8881 agctggatag cataataagg attcagtgca cggataactg tccagtcttg actgaagttc
     8941 attaatccca ttacgcctaa aaaagtaaac caagcgaaca tgattggtcc gaatgctttt
     9001 ccgacaatat ctgtaccaaa gcgttggacc atgaataaaa caaaaataat aactaaagtg
     9061 ataatgacga tcacgttctg gttattaccg aaccggtcaa aaaactgcgg aattcctcgc
     9121 aagccttcaa tcgcagtcgt taccgtcaca gcaggggtca ataccccatc tgctagtaag
     9181 gcagctcctc cgatcattgc aggaatgatc aaatatttcc caccttttcg gacaagtgtg
     9241 tagagtgaga aaattccacc ttcaccatgg ttatctgcat tcaatgcaat cacgacatat
     9301 ttgattgttg tcagtagagt caatgtccaa aaaatcaatg agactgatcc gataacaaaa
     9361 tcaggattga ttcctgacaa tccgccatta tcttcaacaa tcgatttcat aacgtaaagc
     9421 ggacttgtcc cgatgtctcc gtagacgact cccatcgcta caagcaggcc agtcattgat
     9481 atctttttgg tattgtgcgt tgttgccacg tttttccctc atttcttgtt atatggatat
     9541 cctttaggta agcttgaatg atagatgata ttcttatcca tgacacccgc tttcctaaat
     9601 tgcttctaat tatccaagta acacactgaa gtgtcaactt atttgaataa aattattctt
     9661 tctttggacg ttctctgata cgtcttgcca caaatgaata aagagcaagg caaaaaattg
     9721 cacccaataa tgctggtatc aaataaaaac cgccgagttc cggtcccagt ggtcctagta
     9781 aaaattcgcc gagccaacta cctaaaaaac cgataatgat atttccagat acgcctcccg
     9841 ggacttcctt tcctaaaatt gcgccagcta tcaaacctaa tataccgccg ataaataacg
     9901 accataaaaa attcagcatc tcctttccct cctttatcca tatttctcac ttttatttta
     9961 cgctttattc agaaggactc caaaaatatg cgccaagaaa ctccagaaaa aaagaagttg
    10021 taacaaaagt gttcagctta tttccgtaga agctgcttct ggaacaccgt gaatcagagg
    10081 ttgtgaaatc agcggtttga cctgagtatt agagaaacaa acggaaaaat agttcctcat
    10141 attttgccgt tttttgagct aatatcgagg gacagctgat tgaacatcgt ttattggaga
    10201 ggttgtaaaa aagctgttct gcatcaagta ataagaacag ccaaataaaa acagctccta
    10261 atgtttttaa cttggctgga cttattaccg cagatgcaag cttttgaaca ccgtttattc
    10321 gaaaaaaaag gactgtgaca cggcttttgt cacagtcccc ttttttcgaa taattattgt
    10381 ccccctgtca ccttcacgat cacaagtaca ttattcctat gaattaaatt atttttcttc
    10441 atttttcgtt gaaccatctt cgttcaacat tgcttttctt gacagattta cgcgtccttg
    10501 tttatcgatt tcagttactt tcacaaggat ctcatcacct agtttaacaa catcttcgac
    10561 tttattgaca cgatcgtttg ctaattggga aatatggaca agtccatctt ttcctttgat
    10621 caagttgacg aatgcaccga atttttcgat acggacaact ttccctagat aaacttcacc
    10681 aactttgact tctttggtca aatcttcgat gatcttgata gcttttttga tcatttctgc
    10741 atcagctgaa gcaatactta cttttccatc ttgatcgata tcgattttca caccagtttc
    10801 atcgatgatt ccgttgatcg tatcgccgcc tttaccgata acatctttga ttttagcagg
    10861 ttcgatctgg atcatttcga tttttggtgc atattggctt aattcttcac gtggagcagc
    10921 aatcgttgaa gtcaattctt ctaagatttc catacgtgct tgttttgctt gagttaatgc
    10981 ttcagttagg atttgttctg tgattccttg aatcttgata tccatctgta aagcagtgat
    11041 accatctttt gtaccggcaa ctttaaagtc catatcgcca agatgatctt ccagcccttg
    11101 gatatcagtt aggatcgtat agttttcacc atcagaaaca agtcccatcg caataccggc
    11161 gactggcgct ttgattggta cacctgcatc catcaatgct aatgtgccag cacagatact
    11221 tgcttgggaa gaagaaccat ttgattccaa tacttctgct actaaacgga tcgtataagg
    11281 aaattcttct tctgaaggga ttacttgtgc taacgcacgt tcacctaatg cgccatgtcc
    11341 gatttcccga cgccctggag agcctgcacg tcctgtagaa ccaacagaaa attgagggaa
    11401 attgtagtgg tggatgaatc gtttgctgac ttccacccct aaaccatcaa tgatttggtg
    11461 ctctcctaga ggtgcgagtg tacaagctga taaagcttgt gtttgtcctc gtgtgaacaa
    11521 acctgatcca tgtacacgtg gcaataagct gacctctgat gacaatggac ggatttcatc
    11581 caatttacgc ccgtcaggac ggatcttatc gatggtgatc aattcacgta cgacatcttt
    11641 ttcaagatct tctgtgattt gtttaacttc tttcaacaag tgatcttcat cttcatgacc
    11701 tgcaaatttc tcagcataag cttcgcggat atcgatcttc acctgctcga tattatcttc
    11761 gcgagcaagt ttttcttcag tcattactgc tgctttcatt gtagcgtaag aagcatcaaa
    11821 gatttctttt ttcaatactt catcaacttg cagtaaagtc acttccattt tttctttccc
    11881 tactgcttga acgatttctt cttggaaagc aactaactct ttgatagcgt caaaaccaaa
    11941 cagcaatgca ccaagcatgt cttcttctga aacttctttc gcgccgcttt caaccatatt
    12001 gatggcttgt ttcgttccgg caacagtcaa ttcgatatct gtttgttctg cttgttctac
    12061 tgttggattc aagacgtact cgccatttac acgaccaact tctacgcctg cgattgggcc
    12121 gtcaaatggg atatctgaga ttgatagtgc taaagaagaa cctagcattg ctgccatagc
    12181 tggcgagcag tctgtttcta cactcatcac gatgttcgtt acttggactt cattacggaa
    12241 gccttctgca aacatcgggc gaattggacg gtcgatcaaa cgggcagtca atgttgcttc
    12301 cgtacttgga cggccttctc gtttgatgaa acctccaggg attttccctg ctgcatacat
    12361 tttttcttca taattgatcg ttaatgggaa aaagtctgta tcttttgctt ctttagaggc
    12421 cacagctgca cttaagacta ctgtatctcc ataacggact aaaacggctc cgtttgcttg
    12481 ttttgctagc tgaccgactt cgatttgtaa gggacggccg ccccatgtcg ttttgaatac
    12541 ttgtttttct gacataactt ctccttttcc ttctcgtgaa agcaagacgc tactaaacaa
    12601 atgaaaactg ccgaaagccg tcttttgagc agctttcatt tggttagtag gagtccatct
    12661 agcttgatca cggaatttta cttaattgat atacgagtaa aaaaagtgag attcatgtga
    12721 atcccactct ttttgactaa aattaacgac gcaagcctaa acgtttgatc aattcgcggt
    12781 aacgttgaac gtcagtttta cgtaagtaag ctaacaagtt acggcggtga ccaacttttt
    12841 tcattaggcc gcggtatgaa tgatggtctt ttttgtgcac gcgtgcatgt tcattcaagt
    12901 ggttgatttc ttcagttaat acagcgattt gtacttctgg agaaccagta tccccttcat
    12961 ggcgagcgta ttcgttgatg atttcattct tgcgttcttt tgaaattgcc atttctttca
    13021 cctctttcga ttattcccct gcgactgagt aaaacgttgg tgattcgctt aaccaagtgg
    13081 caggtcatgt gcctagagca cacagctata ctttacctga aaccgggttg aaaatcaagt
    13141 agttaatcat taagaaaagg tagttttcaa acaatcttac tactactttt ccatttttgt
    13201 ggtaaactat aatgcgaaaa tagacttgaa acacagtctt ttaagaaagg ggtacatatt
    13261 ccatgattac aatggatgat atcattcgtg aaggaaatcc gaccttacgc gaagttgcaa
    13321 aagaagtttc tcttccatta agcgaagaag atatctcttt aggaaaagag atgcttgagt
    13381 tcttaaaaaa cagccaagat ccaatcaaag ctgaagaact acacttacga ggcggtgttg
    13441 gcttagctgc tcctcaatta gatatttcta aaagaatcat tgctgttcac gtaccaagtc
    13501 ctgacccaga ggcagatgga ccttctctaa gtactgttat gtataatcct aaaattttga
    13561 gccattctgt tcaagatgct tgtttagggg aaggtgaagg ctgtttatct gttgatcgag
    13621 aagtaccagg atatgttgtt cgccatgcca aaatcactgt gtcttattat gatatgaatg
    13681 gtgaaaaaca caagattcgt ttgaagaatt atgaatcgat cgtcgttcaa catgaaatcg
    13741 accatatcaa cggtgtgatg ttctatgacc acatcaacga tcaaaatcca tttgcactaa
    13801 aagaaggcgt attggtgatc gagtagagaa ttggcaggtt ccttcgatac tatgctacaa
    13861 gaaagcttta taaaaaaggg gagtgctcaa aagctgcact tccctttttt atgtttctcc
    13921 actaatcgaa atttagatag acatctagaa atcgcaattc caaaaaattc tgtgtattgg
    13981 atacgcatct tttcttattt tttcttattt cttccatatg ggtgtttatc tcagagatga
    14041 tagaatagtt tctaggataa aaagtagcag ctacgatgtt atccttcgta gaggattcct
    14101 gcttcagcta gtttttgttt gatgtttttc agcatatgct tttctgggta gcggatcgta
    14161 tgcagatgga tgccgcctgt taattcggat aacaaagtag cattactttt ttgataacgt
    14221 ctcataaagg aatcgatatc ttgtggcgtt ttgatgtgca aagttcctgc taactcaccg
    14281 taaaggggat gttccacaat tacatcaatg atttcaccac cattttcaac gattagtctc
    14341 agttcttctt ctgtttgatc ttttccatgc tggacagcga ttttactgat gtgcccagta
    14401 ttggtttctt ctgtttcgag gatataacct cttgctgttg ccacgatact tttacctgca
    14461 gctcgtagta aagcaatatc gcctacaatg atctgacggc tgactccaaa agttttcgca
    14521 aacttgctag cactcactgg tttttctgta tttgccaagg tttgcatgat ttgttttcgc
    14581 cgtgtctctc cttcgatcac acttattcct ccttcatttt gttttggtat tcgcgcgagt
    14641 gtttcatcaa atcaacatac gtatctgtta ttgtttcctt atatccctta tttttcagca
    14701 gactaacaac tttatccaat acttgctgct ctctttttgc atcataaact gcttggtttt
    14761 cttctttttt cacttgtcct attttagtta ccgtatccat tcttttttct aataaagcaa
    14821 tcaactctcg atcgatcata tcgatcttat gacgtaatgt ttcaagagac tcttctgtta
    14881 gttcttcctc tactgtcttg atgatacgag caggatttcc tgcaatcact acattgtcgg
    14941 gaaaagattt ggtcacaacc gaacctgcgc cgactactac gttgtcccct agagtgacac
    15001 ccggcgtgac gataactcct ccgcctaacc aaacattatt accgatttta atgggttttg
    15061 cgtactccag accgctgtta cgtttcactg gatgtagtgg atgcgtcgcc gtgtaaagct
    15121 ggacattcgg agcgaacatg cagttatcac cgatttcaat cgtgctgaca tctaaaaatg
    15181 tacaattgaa atttgcataa aaattctccc caacaaatat attatatccg taatcaaaat
    15241 tgataactgg ctccatatag atttttttcc ctgtacggcc aaaagtctct ttcaacaatt
    15301 gggatcgcag ttccgctgat tctgcttggt tgatgatttg gctttgactt cttgcaaaca
    15361 tacggtcggc aaccagctca ggatcattgg caaaataaag ctcgcctgca atcatttttt
    15421 ctttttcggt tttgaattca gtcataatct gctcctaatt cttgtatttt tttgaaaaca
    15481 accgctccgt cttcttgaat aaacgtataa ggctccgcta aataaacagg cgtttgattg
    15541 attactaaga ttttggctga cggctggcga aactgtacca gatcacaaaa cggatgaact
    15601 tgaaaactcg ttccaacaat gacgatcagc tctgcttggc tgacggcata agccgcctgc
    15661 tccatgactc catcttgaaa cccttcacca tataacacga tgtctgggcg tatttgtcct
    15721 ccgcatgctt gatgtttgtc gctgtgaaga tattctttcc agtctaccgt ttgttgacat
    15781 tttcgacaat gacattgata caaacttcca tgaaaattga cgagttgcgg gcttttagcc
    15841 tttgcgtgca aaccgtcgat gttttgggaa acaacccaaa catttttttc tttcgctaga
    15901 tgtgccattt ccaaatgaat gatattgggt tttgcttcag gatgataaat ctttttgaca
    15961 aattgataga atttatccgg ttcattcacc attgcttgat gactcagcaa gtattccggc
    16021 tgctccatcc cgtggtagat tcctgacaaa gatcgatagt ctggcactcc agaaggtgtg
    16081 gatacccctg caccggtcaa aaatgtaatt tgctgataag ctaatatctc ttttgctgct
    16141 tcgtctactg ttaattgttt catgttttgt cacctcactt tccattatag cccatactcg
    16201 aacaaattta ttgttgaaaa gtcagaaaaa tgggaataaa aaagtgttat gggcatttga
    16261 acaatttatc acaatgttca ctgacaaaag aatgaaaacg tgttacaata ctgctgtata
    16321 aaagtgaggt gaatcgatat gttaccattc tttatttatt ggggcgttat tctggcttgt
    16381 atcgcctggt tagcgttaag cgtttatttc tctgtttttt atcttgtacg caaagaaaat
    16441 ggaaatcttt gggcttttgc tttattcaac gtgattgccg caattgtttt agccattaca
    16501 ttagccgtat atcgaacatg gggctgggga atcacccaat actcaagttt gatttatcta
    16561 atattagcca tttatggcgt cgttgttctt ttgcaagcga tcctaggacg tgagccaaag
    16621 aaagcggcag cttaaatgat cgagatggaa ctggacacag gaaattttct gttctagttc
    16681 tttttttatt gtattacata catttttgtg tataattata ctatcgtttg caatggatag
    16741 atgattcaca ttttgattgt tttttcggta aaagtaaaga tttctatatt tatcagtatt
    16801 ttttcaaaaa acagcagtta tagattgaca aagcctctcg agtatcgtaa actttttttg
    16861 tataaataac tgaatacgag atgcctcaat caatgaggta gaggtcgcga gaatcattaa
    16921 ccctgtggag tgaagcaaac atggagaagc aggaataggg aggatcgccg aaatgtgcac
    16981 ttttttgctt ggagtgtatg ttgggacgca agttaagagc ttgcggactg tcttagcagt
    17041 gatgctaagt tgcgctatgt tttgtgagag gtttatcacc ttatggaat
//
LOCUS       DAZKLA010000053        16297 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000053 DAZKLA010000000
VERSION     DAZKLA010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.054"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(293..658)
                     /locus_tag="ACG85N_001535"
     CDS             complement(293..658)
                     /locus_tag="ACG85N_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224309.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            866..1348
                     /locus_tag="ACG85N_001536"
     CDS             866..1348
                     /locus_tag="ACG85N_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224310.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1545..2435
                     /locus_tag="ACG85N_001537"
     CDS             1545..2435
                     /locus_tag="ACG85N_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFQ0224311.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2760..5231
                     /locus_tag="ACG85N_001538"
     CDS             2760..5231
                     /locus_tag="ACG85N_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0224312.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(5334..7070)
                     /locus_tag="ACG85N_001539"
     CDS             complement(5334..7070)
                     /locus_tag="ACG85N_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0224313.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7067..7771)
                     /locus_tag="ACG85N_001540"
     CDS             complement(7067..7771)
                     /locus_tag="ACG85N_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0224314.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7902..8405
                     /locus_tag="ACG85N_001541"
     CDS             7902..8405
                     /locus_tag="ACG85N_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFQ0224315.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8365..8703
                     /locus_tag="ACG85N_001542"
     CDS             8365..8703
                     /locus_tag="ACG85N_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFQ0224316.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8724..10142
                     /gene="ffh"
                     /locus_tag="ACG85N_001543"
     CDS             8724..10142
                     /gene="ffh"
                     /locus_tag="ACG85N_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFQ0224317.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10254..10832
                     /locus_tag="ACG85N_001544"
     CDS             10254..10832
                     /locus_tag="ACG85N_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFQ0224318.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10836..11357
                     /locus_tag="ACG85N_001545"
     CDS             10836..11357
                     /locus_tag="ACG85N_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFQ0224319.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11436..11990)
                     /locus_tag="ACG85N_001546"
     CDS             complement(11436..11990)
                     /locus_tag="ACG85N_001546"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0224320.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12243..12518
                     /gene="rpsP"
                     /locus_tag="ACG85N_001547"
     CDS             12243..12518
                     /gene="rpsP"
                     /locus_tag="ACG85N_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFQ0224321.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12530..12781
                     /locus_tag="ACG85N_001548"
     CDS             12530..12781
                     /locus_tag="ACG85N_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFQ0224322.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12906..13697
                     /locus_tag="ACG85N_001549"
     CDS             12906..13697
                     /locus_tag="ACG85N_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224323.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13867..14388
                     /gene="rimM"
                     /locus_tag="ACG85N_001550"
     CDS             13867..14388
                     /gene="rimM"
                     /locus_tag="ACG85N_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFQ0224324.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14378..15139
                     /gene="trmD"
                     /locus_tag="ACG85N_001551"
     CDS             14378..15139
                     /gene="trmD"
                     /locus_tag="ACG85N_001551"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFQ0224325.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15275..15622
                     /gene="rplS"
                     /locus_tag="ACG85N_001552"
     CDS             15275..15622
                     /gene="rplS"
                     /locus_tag="ACG85N_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFQ0224326.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(15754..>16297)
                     /locus_tag="ACG85N_001553"
     CDS             complement(15754..>16297)
                     /locus_tag="ACG85N_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0224327.1"
                     /translation="NKRYSLLFQWLYLTGMRSGEAIALSKDDVHITNNNASVVINGTM
                     MYRERSIADMKKSDSTKTAAGMREIDLPKKAIAIYNELLELNPNGQFLFQTTKGTPFQ
                     LTAINTYLRSHKADMKIDKKLSSHIFRHTHISKLAELGTPLYAIQDRVGHENSDITEK
                     IYLHVTKGVKEKLKEDIEKL"
BASE COUNT         5418 a         2908 c         3323 g         4648 t
ORIGIN      
        1 attcaatatc tattattgga atcattattg ctgcaattat tacacaactg tttttagtgt
       61 ttggagagaa gaaaagaagt aaaaaataga aaaactagtg atagttttgg tgatagtgat
      121 attaaaaatc agaaaagata gaaaaagcag gagatagata aaatgctgat atttcagcat
      181 tttttaagag tataaaagag aaaaaaagta ataaatgtat acatgatata agaaaaacat
      241 atatgtataa aaagaagaaa gtagcttctc tttgtacata tatggtttgt ttttatggat
      301 gccgagcttt tttataagat aaggctaccg cccataacga cccaaggatt tgtggaacta
      361 agtaaatacc tgcaagaagc aagatcagct gggtcggttt taaaggatga tagaagtaac
      421 gccattcagt aaaattggcg caaaataaat aggaagtgac agtagatata atattggcaa
      481 tgaataaaga tataatacgc ctgttccttt gaaaataata tccagcaaaa agagataaaa
      541 atatcaatgt gatgaacccg cttacggaat gataacgaaa atcaagcagt aaggcgaaat
      601 gcacataagg aacaaaagat aaaagcaacg gaaggttttt ccagcaagtt tttatcatct
      661 ttcatcacca cctaacaagt gttattattt tgttagtttt attgtaaatt gaatcctgca
      721 ataatcaatt actttttcat tatttctaga ttaaatgaat aaactctcat gccattatct
      781 ctaaaaaaaa agtttttccc cctaattttt ttagatagaa aattgtatag tatgatgaga
      841 gaaaaagtag tttgggggaa cgttaatgag aagaaaagca gtaatcgatc ggctttattt
      901 atttttttgg ttttttccta tcgttttgct accttttaca tgggtaaatg ctgttgtaca
      961 aggtgcggag atattaggcc tgatccaagg aagcggattt gtggtttcta ggaattttcc
     1021 agctttgact gttttctatt tagtgataag tgtaggtctc ttattaccaa aatctctgat
     1081 caatcactac aattggcagc tgctttactt ttcttgctta ttagtattta catctatgtt
     1141 ttctttgctg cctcgcttga ttatcggccc taatggtttg agcgagagtg gattgaagga
     1201 ttcagcttat tcctccatgt atttcttgac gatcaaacca tggttctatt tagtaattgc
     1261 ctcatttatt cttgcaacat ttttttactt ctggacagaa atcaggataa aaagtgaacg
     1321 atctgtatct ataaaaaata actattgatg gtggatcatc agtaaaaaag catatgagct
     1381 gcgtaaaatg catgtttctc atatgctttt tttattatta tgtaaagaat gcgaaaaacg
     1441 tttacatgat ttttacataa cttaactata tttttacagc aaccatacga gggatttaca
     1501 gtcgactgat aaactttagt tgtaaataag aaaggagcaa taaaatgaaa aaagtattgt
     1561 ttactctagg tatgatggga attcttttag ccggatgcgg ctcaggatca ggaaatacag
     1621 atggctctgc cacacaaaat aattcaagtg actcaaatga acaagtaaaa atcgttgcgg
     1681 taggttcgac agcattacag ccgttagtcg atgcagcaca agaaagcttt gtacaagaaa
     1741 atccaaatta tcaaatctct gtacaaggag gaggaagcgg aactggtttg agccaagtcg
     1801 aagctggagc agttactatc ggaaactctg atgtatttgc agaagaaaga gacggagttg
     1861 atgcttcaaa attagtggat cataaagtag cagtggtcgg gatggcgcca atcgtcaata
     1921 aagatacaga tgtcaaagat atcaccaaac aggaattgat tgatatcttt acaggaaaaa
     1981 tcactaactg gaaggaagtt ggcgggaaag atcaaaaaat caacgtcgtg aaccgtgcaa
     2041 acggcagcgg tacccgcgcg acattcgaaa aatggggact ggatggtgct acccctgtgc
     2101 agtcccaaga acaagattca tcaggaacag ttcggcagct ggtaagtcaa acaccaggag
     2161 caatcagcta tttagctttc tcatatctcg atgattccac acaagcacta agtatcgacg
     2221 gtgtagaacc aaaagaagag aatgtagcag ataacagctg gggaatctgg tcatatgaac
     2281 atatgtacac gaacggaaaa ccttcccctg aagtccaaaa attcttagac tacatgatga
     2341 cagaagaaat ccaagaagga cctgtcaaag aattgggcta tttaccaatc acaatgatgg
     2401 aagtagaacg tgatcacgaa ggaaatatca aataactagc agataaacat gccacggtag
     2461 tcgttactat taaggaaaaa tcaactatgt ttcccacata tacatttttc ctttaattgt
     2521 gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc agctgattaa
     2581 acaccgttta ttcgaaaaaa agagctacga catagaactg tcgtagctct ttttttgcta
     2641 tatgacagga atgaaaggtg tttgcaagct taatgtgagg aacactttcc atatctgttc
     2701 tatactggat aggagaagtt tggaaatatc cagaaaacga aatcaatcga ggtagccaca
     2761 tgaagaataa atggataaat cgaagtaaaa aaaagaaagt aacacaaaga tcatcagata
     2821 aagtcatccg tctttttcca tcgtataaag aaggattaac ggcagatcaa gtcaaggaac
     2881 gaatagaaaa aggtgcagca aataattcag tcgatcccac attcaaaaca aatcagcaaa
     2941 tcgtattgga aaacatattt acgtatttca atttgatctt tcttatctta gccattttgc
     3001 tttgtttagt agaatcctat aagaacttga cttttttacc agttatcata gccaatacag
     3061 gaatcgccat ttttcaggaa attcgatcca aaaagatctt ggacgaacta aatgttttaa
     3121 atacagcaaa agtcaatgtc atacgggacg ggatagaaca aacaatagat atagaagacc
     3181 ttgtaataga tgacattgtt attttagaga ctggacatca aattccagca gatgcagagg
     3241 tcgtggaagg aaaactgcaa gttaatgagg ctttattgac aggcgaagca gatgaaataa
     3301 ccaaagaaat aggcgatcca ttaatgtccg gcagtttcat tgtttccgga aaagcttacg
     3361 cccgattaga caaagttgga gcagattcgt atatttccca gctgacagtc aaagccaaga
     3421 caatgggaga aggagaacag tcggagatga ttgcttctct taatcaattg atcaaatgga
     3481 ttgggatcat cattattcct atcggtatcc tgcttttttc tcaaagttac ttttttaatg
     3541 gaaacacgat caaagaaagt gtcgttgcaa tggaagcagc attgatcggt atgatacctg
     3601 aaggactgta cttgctgacg acgattgcat tagcattaag tgcgactaaa ctagcaaagc
     3661 aaagagttct tttacacaat atgaaaagca ttgagacatt ggcaagggta aatgtccttt
     3721 gtgtagataa aacaggaacg atcacagaaa ataaaatgtc tgttcaaaaa gtgatcgtct
     3781 ctaaaaaaca agaaataatc agtcagtcag atcaattgga agcatgcatt gctgactatg
     3841 caaaagcgat ggcaggagac aatgcaacaa tggaagctgt taaagcatat tttacaaaaa
     3901 ccactgatca atcctatcac catgttattc ctttttcttc tgtccaaaaa ttcagcagtg
     3961 tagcgttggc agacaaggtg tatgttttag gagcgcccga aatggttttg cgcgatcaat
     4021 tgccggaata tgcggaagag tttgtttctt tcgctgaaaa aggctatcgc gtgttggtct
     4081 ttggtgtgta cgatgggatt cttgaagaac cagtattgac agaagcagtc acaccattag
     4141 gctatattct gattgctaat cctatcagaa aagaagcaag agccacattt gattacttca
     4201 aaaaacagca agtagccatc aaagtgattt ctggagataa tccgctcacc gtatccaatg
     4261 tagctgtcca agcagggatt tcacatgctg atcagtacgt tgatgcatct aaattatcag
     4321 aagaagaata tgaagcagct gtcgaaaaat atacagtttt tggacgcgtc aagccagaac
     4381 aaaagaaaat cttcgttcaa ttgataaagc gtaacaatac agtagctatg acaggtgatg
     4441 gcgtgaatga tattctagcc atgaaagaag cagactgcag tatagcgatg gcttccggaa
     4501 atgaagccgc catgcaggcc tcacaagtgg tcttacttga ttctgacttt tcacgcatgc
     4561 cagaagttgt tgcagaagga cggcgcgttg tgaacaatat tgagcgatca gccagcttgt
     4621 ttttagtgaa gaatattttc tcctttttgc tctcactgtt ttcagtgatc tttgctctga
     4681 cctatccttt agaaccatca caaatcacat tgattagttt gtttactatc ggacttccat
     4741 ctttcttgct tgctctagaa gaaaacaaaa agcgaatcag agggaaattc atcatgaatg
     4801 tgatggaaaa agctgtgcca ggtggactaa cagatatgat cgtagtcggt gcgttggtga
     4861 tctgcggggt gactttgaat ctaaataaaa cagatgtttc tacagcatct accatgcttt
     4921 tgattgcagt agggttcctc gttctctata aaatctgttc accgctgaat aaattcagaa
     4981 gtcagatcat tcttttctgt gctagcggta actttttttc ggtcatcttt ttgcataagc
     5041 tgttttcaat caccggtatc tcagctgtat cacttttgct gttgagcatt ctatttttct
     5101 cggcagattc gatttttagg catttgacca cattggtaga atatttgttt gaacgtaaaa
     5161 atgaaaaaga gcaaaagccg tttagtcgcg tagctaaaaa atttctatcg atatttaaac
     5221 gagaaaattg acgaaaagta tctcaaaaaa gagtaaaaaa gaacaaccac tcgtgtatct
     5281 ttcgaagcag gatgagtcat ccaactttaa aacgcacgag cagttgctct tttttacatt
     5341 ttcggaatgg taacggtgaa agtcgaacct gttccaagat gactgtctac agtaatcgta
     5401 ccattaagga gttgcacgta gtcttttaca atcgctaacc ctaatccggt cccgcctgaa
     5461 tgtcggcttc gcgccttgtc tacgcggtaa aaacgctcaa agatccgttc ctgatcctca
     5521 atgtcgatac ctataccaaa gtcttggaca gaaaacgata gatcatctgt tgcctgatag
     5581 cggataataa tctcgctgtc gctttttgaa tattggacag cattttcaat cagatttttg
     5641 ataattggat aaaggatatc tgttttagta gtaaatggtt cgttctttcc aatcaatcga
     5701 atcgtcaacc gtttcttttc gatgattggc tgataatttt gaacgatttg ctggaaataa
     5761 ggttctgtat aaattgtctg gatctcgtaa gaattctcgc catcttttga cagctggatg
     5821 atttcccgaa tcagtttatc taaccggatc gcatcttttt gcatgatctc aagaaaagaa
     5881 gtcagagttt gcgggtcatc ttttgcacca tccagtaaag tctcggtaaa accaatcagt
     5941 gaagtcacag gagtttttaa ttcgtgagag acgttgccaa cgaaatcctt ttgtagcttt
     6001 tctaaacgtc gcactttcgt taagtcatac gccaccccta gaatctgtcc ggtaccttct
     6061 gaattagtaa agtagcgcaa actcaagtct aatgtctgcc gaccttctgt aagggtgatc
     6121 tcctgatgga tcagcggatg ctcaggagtc acttgatgaa tcagctgaat gagttttggc
     6181 tcttgaatga cttctgtgta ccgctgttca ggttgatagc ttaccacgtt taaatgtagc
     6241 tgcatctttg gattaagcag tagtaaacga gaatcagcat catcaatcag gaatacgcca
     6301 atcatcagtt cgttcagtaa cgtatataat tgctcttctg tggtagtaca cgcccgatac
     6361 atcttactca tttgttcgct caacgcatta atcgtctgat acaattcttc ccattgatcg
     6421 ggtgtttgca tgattacttc cgcctgatta ggattttgca ccattttttt tagtacgggc
     6481 agcacggttt tcaatggctg gtttttttga tagatcagat aatagatcat tcctgcaaga
     6541 acaaggaaga aagacagaaa gaagaaaaag acccaacggc gaaaactctc agttctcggt
     6601 aaaaaaccgc ttgtcggttc tgcgatacgt aaaatggctt ctaactggcc actttttttg
     6661 actggtaatg cgacatataa aagatcctca ttcaaagtag tacttttgcg caaagcgcta
     6721 cctaggttcc caccattcaa tacggctttg atttcgggac gggagtttcg ctgttcattc
     6781 aatgtttcat tgtttgtgtc aaataagatc tttccagtag catccattaa agtaatccgt
     6841 tcgttcgact gatggacgaa ttcttccaaa gcagttttgt tttgcgctgc ttggaggttt
     6901 tcgacatcta attgccggat cagcagttgt gctttttttg tcaggtagct ttcttgctgt
     6961 tccacgatct gctgttgaaa ataatgggaa atcaattgcc agccaccaaa aaacaaacta
     7021 actaaaagaa taagaaagaa gagacgttca taaatttttt tccatttcat cgttttggtt
     7081 cctgaaactt atagccaaat cctctaatag tcaccaagta ctttggatgt tttggatctc
     7141 gctcgatttt ttctcgcaaa tgactaacgt gtacatctac gattcggctt tgtcctgcga
     7201 agtcaaaatt ccaaatgcgg tctagcaatg tgtctcggtc gatcacacgg tcttttcgtt
     7261 tcatgaaata aacaagtaat tcaaattctt tcggtgttag ttcaatcaga tggtcgtcta
     7321 cggtcacttg ataattggtc aaatcagcag tgatttgtcc aatcgataga tattcaggct
     7381 cagcttcatc ttttgattgc ttacgaggtt caatccgtct gaatattgct ttcatacggg
     7441 ctaatacttc gcgaggacta aatggtttgg tcaaatagtc gtcagcacct atttctaggc
     7501 caatgatccg atccacttgg tcatctttag ccgtcagcat caaaatcggt gtttcatttt
     7561 tttcttgacg cagccgctga gtgatctcca tgccatccat cgttggaagc atcacatcta
     7621 aaatgatgaa atcaaatgat tgttctaatg ctaattctaa tccttcgcct ccatctgtgg
     7681 cactggttac cttgtagcct tctttttcca aattgaacgt caagagcgtt acaatagaag
     7741 gttcatcatc aacaactagt atttttttca ttcaaatctc ctttaaatag gtttcattct
     7801 gtttatattt tagcatagaa tcagtacaaa gggctatttt acaaaagaaa taaatggtat
     7861 aataggaagg cactttgctg aatagaaagg aagagcagtc aatgattgga atcagtgcat
     7921 gtttaggcgg tctggcctgt cgctatgatg gacagaaaaa agaagtacca gaattgatgg
     7981 aactagttga aactggagaa gctgtcatgg tctgtccaga agtgatcggc ggattaccaa
     8041 ttcctagaga tcccgctgaa atcattggtg gaaatggatt cgatgtgtgg aaagatcaag
     8101 caaaagtatg gacgacaagc ggcgaagatg taacggaagc gtacaaaaat ggcgcgatca
     8161 aagcctatca aaaattgcag gaaaaacaca ttgatctagt catcatgaag gaaaaaagcc
     8221 cttcatgcgg aacccattcg atttatgacg gaagtttttc cggaataaaa aaaaatggtg
     8281 ccggagtggc tactgcttat tttattcagc aaaaaatgac tgtgctctct gatgaagaat
     8341 ggctgaaaac tagaggtgag cagaatggaa atcgaaaaga ccaatcgaat gaacgcgctc
     8401 tttgaatttt attcgacctt attgacagaa aaacaaatga actacatgga attatattat
     8461 gcagatgatt tttcattagg tgagatcgcg gaagaatacg aagtaagccg acaagcagta
     8521 tatgacaata tcaagcggac aagcaagatc ttggaggatt atgaaaaaaa actccatctt
     8581 ttttctaatt atattgttcg agaacaggta ttggaaaata tgactgcata tattacagat
     8641 aagtacccgg aagataaaaa actacttgaa tatgtacagc aaattcaagc aatcgaagaa
     8701 taaataaaag gaatgaagaa aatatggcat ttgaaagctt aactgaacgt ctccagcagg
     8761 cgatgggaaa aataagaaaa aaaggaaaag tatcagaagc tgacgtaaaa gaaatgatgc
     8821 gggaaatccg ccttgcctta ctagaagccg atgttaactt gcaagtagtc aaagatttta
     8881 ctaagcgcgt acgcgaacgt gcggtagggg cagaagtgct ggaaagttta tcacctgcac
     8941 aacaaatcgt aaaaatcgtt gatgaagaat tgacgatcac acttggttct gaaacagcag
     9001 agttaaataa atcaccaaaa ataccaactg ttatcatgat ggctggtctt caaggggcag
     9061 gtaaaacgac ctttactggt aaattagcca attatttaaa gaaaaatgaa aatgctcgtc
     9121 cgctcctaat cgcaggggac gtatatcgac cagctgcgat cgaccaattg aaagtattag
     9181 gtcaacagct agacgttcct gtatttgata tgggaactga agtcagcccg gtagagatcg
     9241 tccgtcaagg gatggagtta gcaaaagaaa agaaaaacga ctatgtattg atcgatacgg
     9301 caggacgtct gcatatcgat gaaacattga tggatgagtt gaaacaaatc aaagaattga
     9361 cgcaaccaaa tgagattttg cttgttgtcg atgccatgac tggtcaagat gccgtcaacg
     9421 tagctgatag cttcaaccag caattaggta ttacaggggt cgtcatcact aaacttgatg
     9481 gggatactcg aggcggggca gcactatcga tccgctcagt gacaggcgca ccaatcaaat
     9541 tcatcggttc tggtgaaaaa ttgactgact tagaagtatt ccacccagat cgtatggcta
     9601 gccgtatcct tggtatgggg gatatgctga cattgattga aaaagcgcag caagactacg
     9661 acgagaaaaa agcggaagaa cttgcacaga aaatgcggga aaattcattc gatttcaatg
     9721 actttattga acagcttgat caagtgatgg gtatgggccc actggaagat ctaatcaaaa
     9781 tgatcccagg aatgaatcaa gtccctggaa tcgaaaacgt caaggtagat ccaaaagatg
     9841 ttgaacgcaa aaaagcgatg gtttattcta tgacgccagc agaaagagca aatcctgatc
     9901 tgttgaaccc aagccgtcgt cgccgaattg ctgccggttc tggaaacagt gttgttgaag
     9961 tgaatcgaat gatcaaacaa ttcaaggaat caagaaaaat gatgcagcaa atgtccaaag
    10021 gggatatgaa catcccaggt atggaccaaa tgtttggttc aggcgtcaaa ggaaaactag
    10081 gcaaaatggc gatgaatcga atgatcaaga aaaataagaa gaaaaagaaa aaacggaaat
    10141 aagatgaaaa aaacagcttc ggtacagact gaaaatgtct actgccggag cttttctatc
    10201 agcaattttt attctttact aatacaatta ctctaaacga agaggtggca cagatgagat
    10261 gtccgtggag tgaacaaaca gaaagtatgc ggcaatatca tgatactgtc tggggtgttc
    10321 ctgaatatga tgaccaaaag ctgttccgaa aattgatgct tgatattaat caagccggat
    10381 tgagctggca gacgatttta aataaaagtg aagcattcga tgaagcatat gatcgattcg
    10441 atatcaaaaa agtcgcaaat tatggagaaa caaaaatcag tgaactaatg gaaaacaaag
    10501 gaattattcg taaccgaaga aaaatcgaag cagccgtcca aaacgccaaa gcagtcttga
    10561 aaattcaaga agaaatcggc agtttttcag agtatctttg gtcgttttct aatggaaaaa
    10621 tcgttgattc ttcttatcat tcacaagaag aagtcccaac aacaagtcct ctttcagatg
    10681 aattagcaaa agatctaaag aaaagaggat ttaaatttat cggaagcaca acaatctatg
    10741 cttttttaga agctgtcggg atcatcaatg atcaccttga cagttgtttc cgcaaagaag
    10801 aggtcgtacg tatcggcaga aagggcgaat aaagcatgcg aaaaatcata ttttatggtg
    10861 ctataagttt agatggctat ttggcaacga aaacggatga tcttcaatgg ctttttgaca
    10921 ctccaacagg taagcaaact acttatgaag ctttttatga aacaatcgat acgaccatca
    10981 tgggcagaaa aacttatcag gaagcaaaaa aatatttgga gaccgaagca atttatccag
    11041 agaaaaagaa tttcgttttt tccacccacg cacaattaca attgccagat gctgaggtcg
    11101 taaacgaaga tcctgttgct tttctaaaaa atctacaaat gacagaagga aaagatatct
    11161 gggttgtggg tggcggtcga ttgatcaaac cgctattgga acatcaaatg attgatgaat
    11221 ggtatatcca gattacccct gtattattag gggacgggat tcctttattc caaaaaggaa
    11281 catatgaagc gcgttttgaa atgctagaca cgacacgatt cggtgaattt atcgaattgc
    11341 attatgtacg aaagtagaag taaataaaat aaaaaaggca actatatgca gaaatgtcct
    11401 gtacatagtt gcctttttta ttaccgttat gctttttact ctgttaattt gttagaaaaa
    11461 tctagatacg catctaaaat cgtttggaag aaacttaatg ttccttcaac gatattacca
    11521 ttttcatcaa ttagattcgc aacgtttccg atatatgctt ccggttgctg caaggttggc
    11581 acattcaaaa agacaagtga ttggcgtaga tggtggtttg cgccaaagcc gccgatccca
    11641 cctggtgatg cagtaacgac taaacctggt ttatgatccc aaacactgtg tccataaggt
    11701 cttgagccta catctaaagc atttttcaat actgctggca cagaacggtt gtattcagga
    11761 gtaacgaaaa cgacaccgtc tagtcccctc acttcctcac gaaaacgagt ccattcagca
    11821 ggagcctgtt ctggagtttc gagatcctca ttgtaaaatg gcagatcatc gatttttaca
    11881 aatacacttt cgtacccttc aggaagcaat ttcgcaaaag cttctgctac ttttttgttg
    11941 taagaatctt ttcttaaact accaataaaa aatccaattt tttttgtcat acgatttttc
    12001 ctccttcaaa ctatctataa taatcttaca aaagataagt aactggagca aataaaaaca
    12061 cttactgaca aaagctacat agaagataga ttacttattt ttaacaaaaa agaagtctaa
    12121 aatcttctat tttctaaaaa aaatcgaatt taatcaagct gtaaagaaaa aaagctttac
    12181 agggtaaaaa aaaactgtta cactacatct tgtgaataaa acaatggagg tggaaataaa
    12241 taatggcagt taaaatccgt ttaaaacgta tgggttctaa aaagagtccg ttttaccgta
    12301 tcgtcgtagc tgattcacgt tctcctcgtg atggacgttt catcgaaact gtaggtactt
    12361 acaatccttt gaaagaccct gcagaagtag ttttaaaaga agacttagtt cttgactggt
    12421 tatcaaaagg tgcacaacct tctgacactg tacgtaacat cctttcaaaa gaaggcgtta
    12481 tgcaaaaaca ccacgaagct aaattctcaa agaaataagg tgaccggata tgaaagattt
    12541 aagcgactta gtcttaacaa tcgttcgtcc gctagtcact tatcctgatc aagtcaagtt
    12601 agacgtcgta gagtcgaaag acttttacga gtacaatcta acagtggctc ctgaagatat
    12661 tggccgtatc attggaaaac aagggcgtgt tgctaaagca atccgcacaa tcgtttacgg
    12721 tgttcggatc aatgcaccga aaaaagtgcg tttgaatatc atcgatgata aggaaaagta
    12781 aataaacgtg ctgtactcga attttcgagt gcagcttttt tgtttttttt attaattgaa
    12841 gaaatgatta tttggcaaaa caagcaagaa agcgtatcct tataggagga gggagtgaac
    12901 agccaatgag aaagaaaatg ttatttattc catttgctct attgttaagc gcttgtagtt
    12961 cggttactca agaatcagac aatacacatc tatcaagcag tatatctgaa actgttacca
    13021 gtccagaaaa aagtagtacc aaaacggctt ccaccactga acaaacaatg gctacctcaa
    13081 ataatgagaa aaagactgct ttagaccagt tgaaagaaca acagccaaat gttcccatgc
    13141 ctctagatgt accagtctct tcaggctatc tcaatatcgc agcgacacat acaaagcaag
    13201 gttattctat tttatattac agaacagatc gtcccctcgg gttgaatgct gacgaattga
    13261 atcaagaaac gcctattgca acctatctat atcaatatgg gtttgcttct agtcaagaaa
    13321 caatccaagt tttgcagcca tttgagatcg acacaaatgg tcagcaagtg gacttaggtt
    13381 ctcgaatcac agggtatcag caaggtgcag caggttcaag ttttcttgaa tggcaagaag
    13441 gaaactggcg tattcgaatc cgtgggaata atatagaagg ccaagatccc ttactattag
    13501 ctaaagaaat cgtagcttat ttggaagaaa acagtctacc agcacctgaa caatttggta
    13561 aaattactgt tgatatgggt gatacgacga atcgagccgt agaagtaagc tggcaagaac
    13621 cgaaaaatgc ctatacgatc actcatcaag atcctatgtc tgcgttgaag atggcagttt
    13681 ctatgaagag gttgtgaaat cagcgcgctg atctgagccc atcatttaac tccaagtcac
    13741 aggaagtaat caaaaatagc acgccgcatt tttgcataat tttggcttaa taccttaaaa
    13801 gaaaactaga attgcctctt cacacatgct aaaataaaac agacaaaaaa gaaatgggga
    13861 tataagttga ctgaatactt gaatgtagga aaaatcgtaa acacacaagg tatcaaaggc
    13921 gaagtccgtg ttatctcaac aacggatttc ccagaagaac gttacaaaaa aggaacggtg
    13981 ctaacactat tccaagacgg aaaagcacct gtggaattaa cagtcaaaag tcatcgcaaa
    14041 cataaaaatt ttgacttatt gagttttgaa ggacatcctt ctatcaatga tgtggaaaaa
    14101 tatcgggatg gcatattaaa agtgtcaaaa gacaactctg tagatctagc tgaaaatgaa
    14161 ttctattacc atgagatcat cggtctgcat gtattagaag atggaactga gttaggtaaa
    14221 gtaaaagaaa tcttgtcacc aggtgccaat gatgtttggg tcgttcaacg tccgaaaaaa
    14281 gctgatatat tgcttccata tatcgcttct gtcgtcaaag aagtcgattt agaagctgga
    14341 gtggtccgtg tagaaattcc agaaggactg attgacgatg aagattgatg tattgacttt
    14401 atttccaaga atgtttgaag gtcctatggg ggaatccatc atcggtaagg ctgtagataa
    14461 aggattatta gatatcaata tctctaactt ccgcgattat tcagataaca aacatcagac
    14521 agtagatgat tatccatacg gcggtggagc aggtatgctg ctcaaagtcc agcccatcta
    14581 tgataacatc caagccattg aaaaagctgc accggatgtc aaaaaaagag taattcttct
    14641 tgacccagct ggtaaacgat tcgatcagaa aatggcagaa gaattttcac aagaagaaca
    14701 cttagtattc atttgtggtc attatgaggg atatgatgaa cgtatccgca cattagtgac
    14761 agatgaagtt tccttaggag attatgtcct tactggggga gaattaggtg cgatggtcat
    14821 gatcgatgca acagttcggc ttttgccaga tgtgttaggg aatcagactt cagctcaaac
    14881 ggattcttat tcaaccggtt tattggaaca tccccaatat actcgcccgg cagaatataa
    14941 agggatgaaa gtaccagaag tattgacaaa tggaaatcat aaattgattg aagaatggca
    15001 attaaaagaa tcactaagaa ggacctatca acgtcggcct gatcttttgg aaacacttga
    15061 atggacacct caaatgacga aatttttaga agaaatcaag aaagaagaac aagaaaaaac
    15121 agcagatgaa gccaagtgac tttttctaaa atcttcttta cagtaggttg ataatatgtt
    15181 agtattttat ggtgagtgca aaagcactta actattacga tattccgctg tgagaaaagc
    15241 tcataagaat atttggaata aggagaagaa aaccatgaat ccattaatcg aagaattaac
    15301 aaaagaacaa ttacgttctg acatcccagc gttccgccct ggtgacactg tgcgtgttca
    15361 cgcaaaagtt gttgaaggta ctcgtgaacg tatccagtta tttgaaggtg ttgttatcaa
    15421 acgccgcggt gctggaatca gcgaaactta tacagtacgt aaagtttcta acggagtggg
    15481 cgttgaacgt acattcccat tgcatacacc acgtgttgca aaaatcgaag tagttcgcta
    15541 cggtaaagta cgtcgtgcaa aattgtacta cctacgtgca ttacacggaa aagctgctcg
    15601 tatcaaagaa atccgtcgtt aatcatcgtt atcagacaga gaaagaaccc ttggtattca
    15661 agggttcttt ttttattatc tgttttttta tattaacgat ttctatttgg tacatttttg
    15721 attaataaaa aggggcaaaa aaggggcaac aagttacagt ttttctatat cttctttcaa
    15781 tttttctttt actccttttg tcacatgcaa ataaattttt tcagtgatat cactattttc
    15841 gtgaccaacg cgatcctgaa tggcatacag aggagttcct agttccgcta attttgaaat
    15901 atgggtatgt ctaaaaatat gtgaactaag ttttttatca atcttcatgt cagctttatg
    15961 acttctcaaa taggtgttaa ttgctgttag ttggaaagga gttcctttcg ttgtttgaaa
    16021 tagaaattga ccatttggat ttaattctag aagctcattg taaatagcta tcgctttttt
    16081 tggtaaatca atttctcgca ttccggcagc tgtttttgta gaatcagatt ttttcatatc
    16141 agctattgaa cgttctctat acatcatcgt cccatttata actactgatg cattattatt
    16201 ggtaatgtgt acatcatctt tacttaatgc gattgcttca ccagacctca tcccagtcaa
    16261 atatagccac tggaaaagga gagaatatct tttattg
//
LOCUS       DAZKLA010000054        16220 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000054 DAZKLA010000000
VERSION     DAZKLA010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.055"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..477
                     /gene="scm"
                     /locus_tag="ACG85N_001554"
     CDS             <1..477
                     /gene="scm"
                     /locus_tag="ACG85N_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0224328.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(632..1618)
                     /locus_tag="ACG85N_001555"
     CDS             complement(632..1618)
                     /locus_tag="ACG85N_001555"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0224329.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1641..2207)
                     /locus_tag="ACG85N_001556"
     CDS             complement(1641..2207)
                     /locus_tag="ACG85N_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFQ0224330.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2221..2637)
                     /locus_tag="ACG85N_001557"
     CDS             complement(2221..2637)
                     /locus_tag="ACG85N_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFQ0224331.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3094..5028)
                     /locus_tag="ACG85N_001558"
     CDS             complement(3094..5028)
                     /locus_tag="ACG85N_001558"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0224332.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5330..6265
                     /gene="pplA"
                     /locus_tag="ACG85N_001559"
     CDS             5330..6265
                     /gene="pplA"
                     /locus_tag="ACG85N_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFQ0224333.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6355..7458
                     /locus_tag="ACG85N_001560"
     CDS             6355..7458
                     /locus_tag="ACG85N_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0224334.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7471..8424
                     /gene="menA"
                     /locus_tag="ACG85N_001561"
     CDS             7471..8424
                     /gene="menA"
                     /locus_tag="ACG85N_001561"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFQ0224335.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9238..10584)
                     /gene="gor"
                     /locus_tag="ACG85N_001562"
     CDS             complement(9238..10584)
                     /gene="gor"
                     /locus_tag="ACG85N_001562"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFQ0224336.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10718..11971)
                     /locus_tag="ACG85N_001563"
     CDS             complement(10718..11971)
                     /locus_tag="ACG85N_001563"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFQ0224337.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(11977..12900)
                     /locus_tag="ACG85N_001564"
     CDS             complement(11977..12900)
                     /locus_tag="ACG85N_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFQ0224338.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13121..15613)
                     /locus_tag="ACG85N_001565"
     CDS             complement(13121..15613)
                     /locus_tag="ACG85N_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFQ0224339.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15620..16087)
                     /locus_tag="ACG85N_001566"
     CDS             complement(15620..16087)
                     /locus_tag="ACG85N_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFQ0224340.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4765 a         3394 c         2887 g         5174 t
ORIGIN      
        1 agcacggaca cgtcagcttc aagcgacagt acggatacat ctaccacaac aagtgatagc
       61 acggacacgt cagcttcaag tgacagtaca gatactacct ctaccacaag tgatagctca
      121 gattcatcta ccactacaag tgatagcacg gacacgtcag cttcaagtga cagtacagat
      181 acatcctcta ccacaagtga tagctcagat tcatcaactt caagcgatag tacagacacg
      241 tcgtctacca caagcgatac acgtcatgca agtgtctcct tctcaaaaac aagttcggat
      301 aacaggacga actttaactt aactaataaa acaagcaatg acaatggaaa caaatcaaaa
      361 agagcacttc ctaaaactgg aagtcaatcg aataattgga taacactcgc aggcgttata
      421 ttacttgtaa tcggtttaag aatcactttt atgagttata agaaatcgaa aaggtaaaaa
      481 aatattcaag ataaaaaaga gcaacgtcgc taacggacgt tgctctttat ttttgctttt
      541 tatcataaat acaagaacta caattcaatt tgtcaaaaaa cacgtagatt tccttctgag
      601 aaatttattt acgtatcgaa cctttcccta ttcagtcttt gcgatccaaa atcagttggg
      661 taagggaaaa gagattctct tttgttcgaa gagaggtggc aggtaatttc tttatttcct
      721 tcaaagcctt ttctgtgtaa gaggccgcta acttcctagc ttcttccacg cctcctgtat
      781 gttcaacgat tttgcgtacg gcgtcgacat cttcttctgt cattgccgct ttcttctcta
      841 aataaggtag tagctttggt ttgttttttt gcaaagagta gatcaacggt aaagaataga
      901 ccccttgacg catgtcttct agtacaggtt tgccgatttc ctggctctct tttgtataat
      961 ctaaaatatc atcgataatc tgaaaggcca tcccgataga aagtccgata tctcctgctt
     1021 tttttgcgaa gcgctcgctt gttccacttt caaaagcgcc aacagaacaa cttaattgga
     1081 acagttcagc tgtcttacct gaaatatttt ccaaatattc ttttacagaa agttcataat
     1141 tatagcgatc atccatctgc cctaattcgc cattcaatac tttttccata ctgcgagaat
     1201 tcaattggag acttttcaag gaagtggagt aatcagacaa tagtttaaaa cagcagacaa
     1261 ataaatagtc tcctgcatag acagaaacga cattaccaaa ctgactacgg atattaggct
     1321 gaccacgtcg cagatccgct tcatcaacga tatcatcatg gatcagtgtg gccgtatgga
     1381 gcatttcgat agaagctgcc aaagcgatgg ctttgtctcg atcttgctct ggtccaaatt
     1441 gtgagaacaa tagctgatag gcaggacgca acagctttcc gccggcatgg atcgtttctt
     1501 tgattgcttg ttcgacaggt ttgtttttca ggcggacagt ttcttccatc cgttttagtg
     1561 tttctgtcaa ttctttactt aaagcaggat agttttccca cattggatgt agcttcataa
     1621 aaaaactcct tgtgtgacga ttattttttg gtaaacattg ggctccattt ttgtttggtt
     1681 ccctgctcat aatctttttg gaatcgttga agtgtctgca catgtcgtaa aagataattc
     1741 gctgcgatcc cgattgcaat ccctgataaa atccctaaaa ctgaaaggac tggcaagtac
     1801 aacatgactg tccatgattg tgcaatgaaa gaagccatca aaagctgtcc gacattatgc
     1861 atgaaaccgc cggccgcact aattccgatg atactgacac gttttggtcc taattgcttg
     1921 accaataaca ttccaaaata actaagcaaa gcaccactca tactataaag gaatgtggaa
     1981 atcgttccgc ctagcaaagt agtcaggaat agtcgtagcc aaagtagtaa gaagctgtct
     2041 cgttttttca tggtaaaaat ggcaacgatc gtgattagat tggctaagcc tagttttgca
     2101 ccaggcgcaa aagaaaaagg ataagggatc atattttcca ataaaccaat gatcactcct
     2161 tgtgcaacaa gtagcgaaat ataaatcatt ttttgtaatt tactcatggt gtgtgaaggt
     2221 ctaataaatc agactgtctt cgtcacctcc atctgttgaa cggacttcga tcactaattt
     2281 atgtggcaaa cagacgatgg tttcgccatt tttcgttgcc cagcctcgac gtacgcagat
     2341 ctggtcatcg caatctgctt cttttatccg tactcgatcg ccatctatct cgatcaagtt
     2401 ataatctccg tgttcatctt tgtaagtata tgtgttgctc tcttctccag ccatcaaagg
     2461 gaatgttttg atttctgtgc catccacacg cagtaccgct tctttttctg ctgttgcggt
     2521 gttttgctga agaccaaaaa tcatgactgg taaaaaagaa agaacagtca aaagcaaaat
     2581 aataaaaaca tcccaaggtt tgagcagact tttttcagca aattctttaa ggctcatttt
     2641 tgttctcctt tttccagatt aactcaccca attttagcat aaaaacaaga ggttgtgaca
     2701 cctgtgtctt gctttgagta ataagaacga agaatagaaa acagctttcc atgttttcta
     2761 attctttggg cttattaccg agaagcagca ggtggaacac cgtttataac aagaggttgt
     2821 gacagaagtg ggcaacttca agaaataaga aggaatttac gaaaattact tttcaagttt
     2881 ttgtgaaatt tcggcttatt tttcgaagaa gctacttctg gaacaccgtt tataacaaga
     2941 ggttgtaaca gaggttgtga cacctgtgtc ttgctcatgg aggcttatct cgggcatctt
     3001 attcctttaa ttttacataa aaaggaggct gtgacggttt gtctcagccc ctttctattg
     3061 aaatcttgca ttcaaataac gttctttatc tatttatttc ttgccgtact gggaacttgg
     3121 tctaccgtac caccaattac ctagtgtacg ttgtagccat ggaattaccc agcgatccaa
     3181 cccaatcgca cgtccggaac cgttcatcaa tgcgaaagca acaaaaatga accaaatgtt
     3241 tacccagtaa aacatgcctg ataagcagaa cgtgatcacc aatacaatcg ttgtcgcact
     3301 gcatagccat gtgaataatc cagcaatcaa tgctaatgcc aaggcaattt caaagaatac
     3361 catgaatttt tggaagaaca ttgctacatc attgtttggc atgatcactt gcatgatctt
     3421 tgtgaaccag tctggtgact tctcaatcac agccattggt tcttcaccgt atgcataact
     3481 caaaccaaaa tgtgcagctt gagtcgctgc ttctccggca cttgcccctg ctccgctggc
     3541 agctgtggtt gcatcaccag cagcagaagc accactggca acatctgtta cattgtattc
     3601 ccatgtccaa gggaacacac tgtttccagt gaaccatgat ccttcaccaa accaagtact
     3661 tggtttgaac acttcaccat tcccaatgat ttttttggaa gcttcaacga accaaactaa
     3721 accatagaaa atacgaagtg gcacactcca taagacattg ccgtaacgag aagaatgtcc
     3781 tcgagtcacg ttgcgatcat ctttgatatg gaaaaactca tgcattaaat attggaacat
     3841 ataataacca gaacgaatat caaagaagta tttcaaattc acgatatgtt tcattacgat
     3901 agctaaaaat ccgcttaaat ggatcttatc aaataggtta gctacccccc atttggcacc
     3961 aatagaaacc ataaatcctt gatagtttcc tttaaattta tgcttttctc cgcctttgat
     4021 cgaagccacg atattcgctg cggctgtgtg tcctgtttgt tcagcagctt gaacgatctg
     4081 cggtgtcggt gttcctggga attcttcata aaagactaaa tcaccaatga tatagatgtt
     4141 tttatcttcg tagccttttg cctgcatata ttcattggcg atcaaccgct gtccgcgtgc
     4201 tgattctaat ccaaagtcag cagcgtcaga agttcctttg acaccagctg tccaaatcag
     4261 tgtgtgagtc ggaatgttgc gtccgtcttt caatttgata tgatcagcag ccacttctac
     4321 gattggtgca ttcaataata attccacgtt gtgcttacgc aaatagcgtt cagcttttgc
     4381 agcatcattt cgatccaaca tattcaagat cgtcggcatt gcctctacga ccatcagtgt
     4441 gatttcgttt ggatctaatt tataatcttt ggctaggcga tttttccagt cgatcagttc
     4501 cccaatcatt tcaataccgg taaatccaga accacagaca acgaaagtca gcatggcttt
     4561 acgcagttca tcatctggtt ccaatgcagc ttttgctact gtcttctcaa tatgttcccg
     4621 aatccttaca gaatcttcaa atgaccaaag ggtgaatccg tgtgctttga caccaggtgt
     4681 accaaagtca ttcggctctc cgcccattcc aaggatcagc tgatcaaaag gataggaacc
     4741 taatttggtc ttgacgattt ttcgttcttt atcgataccg attacagtgt ctgtgacaag
     4801 ttgtacattc ttttgacgag caaacaaatg ctgcagatcg tattggattg cttctggttc
     4861 tacacgtccg ccagctactt catgcaactc ggtcatcata gtatggtacg aatgacgatc
     4921 gatcagagta attgctacat cttctttttt cagtttcttt gccaagtatt ttgtagctga
     4981 aacaccggca tatcctgctc ctacaacgac aatattttgc tttgccattg atcatccgct
     5041 ccttatagac tatctatata atgaatatat gataattctt ttagtttgat acttattaca
     5101 caatctccat atattatata gatgttctgt tcttttgtct aactcctttt ggattttttt
     5161 caaattttaa aataaagcgt tttttcacga aatttgctga aagaaaacgt ttgaaatatt
     5221 acatgggtta tttatcaaaa atagttgact tataggctaa aaagattaga atgtataatg
     5281 aatcacaaag aaaaattgtg gactagaaat ggaatggtgg ggtattaaaa tgaagaaaaa
     5341 acgttttgta actggatttg ccgtattagc attttcagct ctagtgttag gcgcttgcgg
     5401 cgcagatgac aacggaagct caaactcttc ttctgaatca tctacagcac aatcttcgac
     5461 tgcaaaaagc acaacagaat catctgcaaa agttgtagct ggcggagatt tgcaagacgg
     5521 cacatataaa ttagaagaaa aaaattattc aaatggttac cgtgcagtct ttgaaatggt
     5581 tgtaaaagac ggcaaaatca ctgaatcaaa atatgataac gtcaatgaaa acggcgaatc
     5641 aaaaacaaaa gatgcagaat acaacaaaaa catggaagca aaatctggca caaacccaga
     5701 aaaatacatt cctgagttga atgaccaatt cttaaaagca caaagcgcaa gcggtgtgga
     5761 agtcgtaact ggagctactc actcatctga aagcttccaa aactacgcac aacaattgat
     5821 ccaagctgct caagctggta acacagatac aatcgaaatc gacaatggtg ctgatttgaa
     5881 agacggcact tacaagcttg aagaaaagaa ctactctaac ggctaccgtg tgcaattcga
     5941 aatgacagtt gctggcggca aagtaactga atcaaacttt gattatatcg acaaagacgg
     6001 taaatcaaaa caagatgata cagaatataa tgaaaatatg aaagcaaaaa gcggtacaga
     6061 accaaaaact tacatcccta ctctgaacga cgaattcgtc aaagcaatgg gcgaagaaga
     6121 tggctcacct gctaatgtag aagttgttac aggtgctaca cacagctcac attcattcat
     6181 catgtacgca caacaattag tgaatgcagc tgaaaaaggc gacactcaaa caatcgaagt
     6241 agataatatc gtaacagaaa aataatcaca taatcatact aataaaagtt ggagggtggg
     6301 acgatagcca gattaggcag tcgtctcacc tttcaaaatg aagagaggaa aaagatgcgg
     6361 aaaaaactac tattttcctt aatcacactg ggcatctctt ttatggtgct aactggctgc
     6421 agcaactcag caaataaaac aaccactgaa agttcagcag ctaccaagat attgaaagat
     6481 ccatattctg atgaacagtt cttgttaggg acttacgttc gtatccgtgt gtatgatgaa
     6541 ggcaaagaat cagcattgaa acctgccttc gatcgagtaa aagaacttgg tgataaaatc
     6601 acgatcaatc aaaaaggttc tgagatcgat gaagtgaatg agcaagctgg gatcaaacca
     6661 gtaaaagttt ctgatgacgt ctacacgtta gtcaagcggg cctacgaata tagccaagat
     6721 tctcaaggcg gattcgatat ggcgattggt gcaatcacac aattatggcg aatcggtttt
     6781 gatgacgcca gaaaaccttc ccaagaagaa atcgatcagg cattgaaatt agtggattat
     6841 cataagatcg aactaaatga caaagaaaaa actgtttatc taaaagaaaa aggaatgatc
     6901 atcgatttag gtgcaatcgc caaaggttac attacggacg aagtagtgaa agtcttgaaa
     6961 aaacaaggag tgaccactgc catcgttgac ttgggcggta atgtctatgt cttaggccac
     7021 agcccaagag gcgaaaacca agactggaca gtcggcatcc aagatccgaa catggctcgc
     7081 ggatctgttt taggttctat taaagaacgg aacaaaacat tagttacctc tggaatttac
     7141 gagcgttatt tagaagtaga tggcaaaaaa taccatcatt tgtttgatcc aaaaacgggt
     7201 tatccgttcg acaatgatat cgcaagtgtc acgatcataa cagataaatc aattgatggt
     7261 gacgggttgt ctacagcagt cttttcaatg ggtgtgaaaa aaggactgga atacgtcgaa
     7321 agcgaattga ataacgggac ggaagcgatc ttcgtgacaa aagacgataa agtatatgtg
     7381 accgatccaa tcaaagacac cttcaaatta tcggaggact cccattacac gatgggtgac
     7441 cgttctgaac taaaataagg agtacgaacc atgacactaa aaatcttttt agaagtagtt
     7501 gaaatacgaa caaaggtggc tagtgtattt ccttttgcgg taggggtatt attctctatc
     7561 gctttttttc atgagataca ttggctaaac acttttttat tttttattgg aatgctagtt
     7621 tttgatttag cgactaccgc tatcaataat tacatggatt atgaaaaggc tcattctgaa
     7681 gtatataagt atgaagaaaa tgtcattggc cgcagaaatc tatctcctgt attgattcga
     7741 aacatgattt tagctatgat tgcatttgtt tttgttatcg gcgttattct aacgatcaat
     7801 acgggttggc ttttactttt gatgggattt atctgttgtt ttattggagt attctataca
     7861 ttcggaccga ttcctttatc tcgaatgcct ttaggggaaa ttttcagtgg ggtgactatg
     7921 ggccttggaa tttttgccat ggtcgtttat gtcaatacat tcggttcaga tgtctatgat
     7981 ctgacagtca acttttcatc tggacaattt gctttggtag gaaatctttg ggctatagca
     8041 gcaatcgtat tagcttcctt acccttggtg tttacgattg ccaatatcat gttggctaat
     8101 aatttgcggg atcttgaccg tgatattgaa aatcatcgct atacactagt tttttatatc
     8161 ggacgtcctg cgggaacagt cttattccag ctattgatgt atgcttgcta tttcgtgatt
     8221 ttaatggggt tgttgagcgg tatctatgaa tggccgatct tggtcacctt cttgaccttg
     8281 ccaattgtga agaagcattt ggatcaattc aagcaaacgt taccccagcc tatcagtttc
     8341 agctattcca tcaaaaatat ggtcttattc aatgccagtt atgtattggg gctattacta
     8401 acaatcatta tgcaagccat ttaacagcat gaagaggttg tgaaaaaact gccctgcatc
     8461 aagtaataag aacagccaaa taaaaatagc tccttatatt tttcgtttgg ttgggcttat
     8521 taccgcagat gcaagctttt gaacaccgtt tatcaggagg ttgtgaaaaa gtcgcttagt
     8581 ttcgagtatt aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttggct
     8641 taataccgag aaactggctt ttgaacaccg tttatcagag gttgtaaaat cagtgttttg
     8701 tcctgagtat tagagaaaca aatggaaaaa tagttcctca tattttgccg ttttttgagc
     8761 taataccgaa ggacagctga ttgaacacta tttgttcgga aaaagtgact aactatgaca
     8821 caattttttt cacagtccct tttttcactt gatttgattc gtaccagcga acttgacgag
     8881 aactttcttg gtttggtaac tatatggtat tcttttaaca tcctcgtttg cttcttctaa
     8941 tttatttata caaagtcaca cttttttaaa catctgttct tcgatacatt tctatttctt
     9001 ttaaacatcg ataccaatat tttgaactta agagtgcata ccaaatggat tcatttactc
     9061 ttcgtcagtt acgaagataa tcaaaatctc caacttccct acttatttcg ttttgagaat
     9121 tatcgaagca atcttacata aataactaaa aaacaaaaaa ccagaaaatc agtatactga
     9181 ttttctggtt ttacatggtt tgttcaataa aaagctgttg gaactgttaa tattaaatta
     9241 tcgcatcgtt acgaattctt ccgctcctgt tggatggatg gctactgtat tgtcaaagtc
     9301 ttctttagtt gcacccatct tgatagccac ggcaaaacct tgcagcatct catcgacacc
     9361 aacaccaata gcatgtaagc cgacgattct ttcttctttt ccgacacaga tcaatttcat
     9421 ttcgcatttt tggcgataat catttaatgc aaagtacatt ggggtaaagc gtgaacgata
     9481 aactttcaat tcattttcgc catatttttc caatgcttct tcttctgtca aaccaattgt
     9541 tgcaattggt ggatgcgtaa agacgactgt cggaacaagt ttatagtcaa gataagaatc
     9601 tttctttcca ttaaataaac gttctgataa acgtctacca gctgcgattg caacaggagt
     9661 cagatctaat ttaccgatca catcaccaac agcaaaaata tgttcatccg ttgtattttg
     9721 gtatttatct actttgataa accctttttt gtccaattcc acatttgtat tttccagacc
     9781 taatttttcg gtattcggta cacgtccacc ggcaaaaagt acacaatcac cagttaaggt
     9841 ttcaccattt tcaaatagca ccgtatattc gtcattgttt ttagtaattt ctttagcaat
     9901 atgatgtgcg taagttttta ttccagcttc ttggtacatt tggatcaaat tgtccgaaag
     9961 cattttatca aacgttctaa gtggtcgttc ttttcgatat gcccacatga cttcactacc
    10021 taaaccacga aatacacctg aaagttcagc cgcaatgtat ccagcaccta aaacgattgc
    10081 tctcttaggt aactctgtta attcaaaaaa tccatttgaa tcaattgcgt attctccacc
    10141 aggaatggtc attttacttg cacggcctcc agtagcgatc aagattttag gagcacgata
    10201 ggtttcaccg tttacttcga tcgtttgact atcgataaaa gtagcgtaac cttttatagc
    10261 ctcgacatga ttgctatcta atcctctttg ataagcaccg tgtagaaaat caatatattt
    10321 ttctcgtctt tctactagac cggcgaaatc aacatttttt aaatctgctt ggataccgta
    10381 acttgaggca tctcgttcaa ttgtttccaa aatcgtactt gcttgccaca taactttttt
    10441 aggtacacaa ccgacgttga cacatgttcc gcccaattca ttcgcttcaa tcagcaaaac
    10501 ttttgcgcca tgcatccctg ctctgtttgc tgaagcaata ccgccagagc cgccaccaat
    10561 tacgatgtaa tcatatattt gcattttgtt cctccttatt gaagagatca atcaaaacct
    10621 ttatttaacg atcctttttc tacagaaaac aagtgtaaca cttttcttga attttgtatg
    10681 cgaagatgct taaaacaggg atatatcagc tttttcctta tttacgtttt cggatcatat
    10741 ctccaggttt caccggctgt gcgactggca tagttaaaat catcattgga tttggtgctc
    10801 ggtcaatgga ttcgccttct tcattatgca taacttcaac ggtttgactg aaatgactga
    10861 atcctggacc ataaaattcg atttcatctc ctacagaaaa caagtttctc tgacgtatcg
    10921 tagcaatctg tgttgcctca tcataagata atacttcacc gacaaattta tatactggga
    10981 ttttacgacg ttcaccgaat aattgttcgt tttcagttgg tgtattgtag taaaaacctg
    11041 tagctaattc tcgttgtgct actttccata actcgtcgat ccattcttgc ttgcagacat
    11101 agttttcagg atcttccata taactgtcta cagccgcttt atagacattt gacaccgtag
    11161 atacataatg gatcgatttc attcttcctt cgatcttgaa gctgtctacg ccgttttcaa
    11221 tcaattctgg aatatgttcg atcattgaca tatccactgc actcatcgag aattcttctt
    11281 ccacttcgcc tgtatcagtt aaactgtttc gttcttgacc aaaaggcata tcgaacaatc
    11341 catacttcca gcgacaagat tgagaacatc cgccacggtt tgcatcccgc atcgacatat
    11401 ggttggacaa tgtgcatcga ccagaataag agatacacat ggcaccatgg ataaatgctt
    11461 cgatttcggt atccgtattt ttacgaatct ctgccacttc ctccattgaa acttcacgag
    11521 ctaaaacgac acgttccaac ccttcttctt tccagaattc caatgtctca aaattagttg
    11581 ctgaagcttg agtggataaa tggatcggca gacctggggc ttctgtcgca caaatttcga
    11641 tcaaagctgg atcagaaaca atcactgcag aaataccgac atcacgcaaa ctgcggaaaa
    11701 attcccctgc tccatcttca tttccttcat gggtgaccat attagcagca acgtatacct
    11761 tcgcatcatg ttcttttgca aaagcaactc cttcagccat ttcttcataa gtaaaattac
    11821 ctgcacggct tcttaaacca tatgcattcc cgccaatata tactgcatct gctccataat
    11881 ggatcgcggt ttttaacttt tccagtgttc ctgctggcgc taggatttct ggtcgtttca
    11941 attttctttt tactgtcatt tctttcatca tactccttat ttgatagcag aaggatcaat
    12001 ataaaagaat cctgtatcta attcacggtt ttcaggatgc aatttactga tttcgctagt
    12061 cattttttcc gctttttctt gatcccagct accttctaaa atttgttttt ttgcttcggc
    12121 gaatagtcga gtgatgttta cgaatttttc gcctggagta aataatccgt ccaatttcca
    12181 tgtacagtaa tgatgctgat aaagttcatt caattcattc gagagattca gatcattgct
    12241 ggcaaaaata tgtgtgccat gactatcttc ataaatagaa taatgtgtct cttctttttt
    12301 aggttccgag ataaaaagac ctctttcacg atctttttgt tcgtcttgtt tcgtatagtt
    12361 atagtaattc tgtagcagtg gacgtttgga ttgatggatg catgtagcac catacacaag
    12421 ggtttctaca ggaatatcga gtttttcttc catcgccttc atctcttcaa aaggaacttc
    12481 acgagcaagt actgctccaa ctgcgccttt tttcgcccag aaattgattt gacgtgcgct
    12541 cgttactaat gtttcagcat cataaatata cggtatacgc aattcaggat tttttttcat
    12601 cgtataaata atacctgtat caccaactgt gatcacatct acgtttattt cttttaaaaa
    12661 agccaaatat tcaggtacat ttttcatttt ttcaggatga atgatcccat ttacagcaat
    12721 attcgctttt ttaccatact tgtgtgctaa ttctaccaat tgtcgctgtt cttctcgtgt
    12781 aaatgaagca ggcagacgca atccaaattg ctcttcaccg aaatatagtg tatctgcacc
    12841 tgcagcaagc aactgttctg cttgttcaat agtttctact gtggttatga gttcgatcat
    12901 aattttctcc tcctacaact tcataatagt agcatagctc taaaaacttt ccaatacttc
    12961 ttctatttaa agtcggattt ttgaaaatta tttcacaaaa aatcaaaata aagttcgata
    13021 tattctgtcc aaatataaaa agagattgtg acaaaaatct tgtcacaacc tcttaatcaa
    13081 gtcttactat tataaaaatt cagctacaca ggccaagtaa ctatagggaa cctactgttt
    13141 cctttttagg tccgcgaaca ttcaatgtga tcttaccttt tgaagcacct actgttacgt
    13201 ggtcacctaa acgaatttct ccgccaagca aagcctcgct caatcgatct tcaatttctt
    13261 tttgaagtgc gcgacggatt ggacgtgcac catattcagg atcaaatcca gctttcccga
    13321 tcacatcgat tgcagatggt gtgattttca cttgaatatc ttgttctttc aatcgtttga
    13381 taatcgcttt gctcatgatt tggacgattg tatggatttc atctttactc aatgaatgga
    13441 agacgacagt ctcatcgatt cgattcaaga attcaggtcg gaaagctttc tttaactctt
    13501 ctaagatacg tttttgcatg gctttgtgat ctttagtaac atcttggaca ttgaatccga
    13561 catttttttc ttctcgaatt tgtgtcgctc cgatatttga agtcataatc atgattgtat
    13621 ttctaaagtc cacttttcta cctttagaat ctgttaaatg accatcatcc aatacttgca
    13681 gaagtaaatt gaaaacgtct ggatgtgctt tctccacttc atctaagaga atcactgaat
    13741 atggtttttg acgaattttt tctgtcaact gtccaccttc atcataccct acgtatcctg
    13801 gaggagaccc gattaatcgg cttgtactgt atttttccat aaattctgac atatcaacac
    13861 ggatcagggc atcttcactt ccaaacatga cttctgaaag tgctttggcc aattctgttt
    13921 ttccgacacc agttggtcca aggaacatga atgaaccaat tggacgatca ggatctttca
    13981 atccgcttcg tgcacggcgg atcgcacgtg aaactgcttt gacagcctca tcttgaccaa
    14041 cgacacgttc atgcagaagt ccttccagtt ctaacaagcg ttcgctttct tttttctcca
    14101 actgctgtag agggacacca gtccactccg atacaactgt agcaacatct tcttctgtta
    14161 cacgatcaga ataaccttgc tcctgttttg cttcaattgc agaaactttt tgcagctttt
    14221 tacgtagtgc tttttcttgt ctgcgtaatt gagcagcatt ttcaaagtct tgtttttgga
    14281 tcgctgcttc cttttcttgt actagttcat cgatttctag tttgatcaca gttgattttg
    14341 ttaagtgatc ggtttgatcc aaacggactt tcgctgctga ttcatcgatc aaatcaattg
    14401 ctttatcggg tagttgacga gaagtgatat aacgaacaga aagattgact gctgctcgta
    14461 cagcttcatc tgtgatctcc actccatggt gctcttcata tcttgtacgt aatccttgaa
    14521 gaatcacttc tgcttcttca ggagttggct catctacttg aatgcgagca aaacgtcgct
    14581 ctaatgcaga atctttttcg atatattttt gatattcatc tagggtcgtt gctccgatcg
    14641 tttgtaattc tcctcgagct aacgccggtt tcaagatatt cgaagcatca attgctcctt
    14701 ctgctccacc tgcaccaatt aaggtatgca attcatcaat gaataagatt acttgaccat
    14761 cattatagat ttcgtcgatg acctttttca ttcgatcttc aaattcaccg cgatatttcg
    14821 taccagcaac caaagatccc atatcaagca tcatcaggcg cttttcttgc atgtcttctg
    14881 gcacttcacc taaaatgatt ttttgagcta acccttcagc aatggctgtt ttgcctacac
    14941 caggttcacc caccagtaca gggttattct ttgtccgtcg gctaagaatc tggataagcc
    15001 gtttcacttc tctcgaacga ccaacaactg ggtccagctt gttttctttt gctaatttcg
    15061 tcaaatcacg tgctaatgaa tctagcgtag gtgttccttg cggtgtttgc cgtttatctt
    15121 gtacatttac atttctacga cgattcattc cttttccagc agaatcacca gctagcccca
    15181 ttttttgttt cagaacttga cgggcttttg aaaggctgat acctaagttg atcatgattc
    15241 gagtagccat gatactttca tcttttaata agctcatcaa aatatgttct gttccaactt
    15301 tttgagaacc agttttcttc acttcgctgc tagctaaagc aaaaacttgt ttcattcttg
    15361 gtgaatatgg aagaaagact cctttagggt aagaacggat accaccataa ccaatcagat
    15421 gttcaatttc ttcataaatt tccgtttcat tcaaatccat ttcacgtaat acttttccag
    15481 caattccatt ttgctcgacc actaaggcta gtaaaacatg ttctgaacta actgattgat
    15541 gtttcaaccg ttttgcttct tcttgtgcaa tttctaatac tgcttgggct ctttcagtaa
    15601 aaagttcttc catctaatct cacttcccat cttcataact taaacgttcc agaaatgctc
    15661 ggagaatccg agcacgtgta tgatcttcat attcattgaa attcaatgtg ttttttgcaa
    15721 ttgcactcaa catcaagttt ccttcttttt ttgtaattat ttggtcttca taaagttttt
    15781 gaataatagc aaaagcattt ttctcactga ttgtatcatc aaataactga tttatctgct
    15841 caagtaattg ttttttatct gaaatcctta cttttgcaat tcgtatataa ccaccaccac
    15901 cacgtttgct ttctactaga taaccacgtt ggatggtaaa acgggtatta atcacatagt
    15961 tgatttgtga aggaacacag ttaaagaggt ctgccatttc aatccttcga atctcgatca
    16021 tttcactatt ttccagaatt ttctttatat atgattcaat taaatccgag gtattctgat
    16081 gtgccatctt attcattccc tttgactaat attgacctta attataacga atttgataat
    16141 agaaagaaag aagaacgctt tttgttaaat aaatccttta gaatcaacaa aaaaaaatac
    16201 ggaatatact attccgtatc
//
LOCUS       DAZKLA010000055        16034 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000055 DAZKLA010000000
VERSION     DAZKLA010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.056"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACG85N_001567"
     CDS             226..894
                     /locus_tag="ACG85N_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFQ0224341.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1414)
                     /locus_tag="ACG85N_001568"
     CDS             complement(1094..1414)
                     /locus_tag="ACG85N_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224342.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1451..2833)
                     /locus_tag="ACG85N_001569"
     CDS             complement(1451..2833)
                     /locus_tag="ACG85N_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0224343.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="ACG85N_001570"
     CDS             complement(2989..4176)
                     /locus_tag="ACG85N_001570"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFQ0224344.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="ACG85N_001571"
     CDS             complement(4183..5214)
                     /locus_tag="ACG85N_001571"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0224345.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5230..6684)
                     /locus_tag="ACG85N_001572"
     CDS             complement(5230..6684)
                     /locus_tag="ACG85N_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFQ0224346.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            6859..7764
                     /locus_tag="ACG85N_001573"
     CDS             6859..7764
                     /locus_tag="ACG85N_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFQ0224347.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="ACG85N_001574"
     CDS             7855..8532
                     /locus_tag="ACG85N_001574"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0224348.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="ACG85N_001575"
     CDS             8529..8699
                     /locus_tag="ACG85N_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224349.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="ACG85N_001576"
     CDS             8813..9709
                     /locus_tag="ACG85N_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0224350.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="ACG85N_001577"
     CDS             complement(9764..11134)
                     /locus_tag="ACG85N_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0224351.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="ACG85N_001578"
     CDS             11372..12118
                     /locus_tag="ACG85N_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224352.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="ACG85N_001579"
     CDS             12115..12954
                     /locus_tag="ACG85N_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0224353.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="ACG85N_001580"
     CDS             12955..13905
                     /locus_tag="ACG85N_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0224354.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="ACG85N_001581"
     CDS             14046..14798
                     /locus_tag="ACG85N_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224355.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="ACG85N_001582"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="ACG85N_001582"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFQ0224356.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5198 a         3001 c         3229 g         4606 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gatctactgc atcgtgaagt tcatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa cattattcaa agtttttgtc cagtaattgt tgtaacaatt
    16021 actggttttt ttgt
//
LOCUS       DAZKLA010000056        15742 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000056 DAZKLA010000000
VERSION     DAZKLA010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.057"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..722)
                     /locus_tag="ACG85N_001583"
     CDS             complement(24..722)
                     /locus_tag="ACG85N_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFQ0224357.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="ACG85N_001584"
     CDS             complement(715..2067)
                     /locus_tag="ACG85N_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFQ0224358.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="ACG85N_001585"
     CDS             complement(2290..3690)
                     /locus_tag="ACG85N_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0224359.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="ACG85N_001586"
     CDS             3851..4588
                     /locus_tag="ACG85N_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFQ0224360.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="ACG85N_001587"
     CDS             4713..5192
                     /locus_tag="ACG85N_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFQ0224361.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="ACG85N_001588"
     CDS             5189..5956
                     /locus_tag="ACG85N_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFQ0224362.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="ACG85N_001589"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="ACG85N_001589"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0224363.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="ACG85N_001590"
     CDS             complement(6859..7461)
                     /locus_tag="ACG85N_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0224364.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..7797
                     /locus_tag="ACG85N_001591"
     CDS             7567..7797
                     /locus_tag="ACG85N_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224365.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7788..8036
                     /locus_tag="ACG85N_001592"
     CDS             7788..8036
                     /locus_tag="ACG85N_001592"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0224366.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8142..8726
                     /locus_tag="ACG85N_001593"
     CDS             8142..8726
                     /locus_tag="ACG85N_001593"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFQ0224367.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="ACG85N_001594"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="ACG85N_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFQ0224368.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="ACG85N_001595"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="ACG85N_001595"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFQ0224369.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="ACG85N_001596"
     CDS             10627..10806
                     /locus_tag="ACG85N_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224370.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="ACG85N_001597"
     CDS             10828..11853
                     /locus_tag="ACG85N_001597"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFQ0224371.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /gene="glyA"
                     /locus_tag="ACG85N_001598"
     CDS             12028..13272
                     /gene="glyA"
                     /locus_tag="ACG85N_001598"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFQ0224372.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="ACG85N_001599"
     CDS             13425..14372
                     /locus_tag="ACG85N_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFQ0224373.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="ACG85N_001600"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="ACG85N_001600"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFQ0224374.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="ACG85N_001601"
     CDS             complement(15274..15516)
                     /locus_tag="ACG85N_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224375.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="ACG85N_001602"
     tRNA            15637..15709
                     /locus_tag="ACG85N_001602"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
BASE COUNT         5349 a         2823 c         3195 g         4375 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt tt
//
LOCUS       DAZKLA010000057        15730 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000057 DAZKLA010000000
VERSION     DAZKLA010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.058"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            92..1519
                     /locus_tag="ACG85N_001603"
     CDS             92..1519
                     /locus_tag="ACG85N_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0224376.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1611..1976)
                     /locus_tag="ACG85N_001604"
     CDS             complement(1611..1976)
                     /locus_tag="ACG85N_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0224377.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1973..2338)
                     /locus_tag="ACG85N_001605"
     CDS             complement(1973..2338)
                     /locus_tag="ACG85N_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0224378.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2530..2778)
                     /locus_tag="ACG85N_001606"
     CDS             complement(2530..2778)
                     /locus_tag="ACG85N_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224379.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2777..2905
                     /locus_tag="ACG85N_001607"
     CDS             2777..2905
                     /locus_tag="ACG85N_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFQ0224380.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2962..4656
                     /locus_tag="ACG85N_001608"
     CDS             2962..4656
                     /locus_tag="ACG85N_001608"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFQ0224381.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4723..4929
                     /locus_tag="ACG85N_001609"
     CDS             4723..4929
                     /locus_tag="ACG85N_001609"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224382.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5307..6440
                     /locus_tag="ACG85N_001610"
     CDS             5307..6440
                     /locus_tag="ACG85N_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFQ0224383.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6471..6791
                     /locus_tag="ACG85N_001611"
     CDS             6471..6791
                     /locus_tag="ACG85N_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFQ0224384.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6820..7284)
                     /locus_tag="ACG85N_001612"
     CDS             complement(6820..7284)
                     /locus_tag="ACG85N_001612"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0224385.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7416..8669)
                     /locus_tag="ACG85N_001613"
     CDS             complement(7416..8669)
                     /locus_tag="ACG85N_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0224386.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8698..9015)
                     /locus_tag="ACG85N_001614"
     CDS             complement(8698..9015)
                     /locus_tag="ACG85N_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0224387.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9041..9361)
                     /locus_tag="ACG85N_001615"
     CDS             complement(9041..9361)
                     /locus_tag="ACG85N_001615"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0224388.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9825..11240)
                     /locus_tag="ACG85N_001616"
     CDS             complement(9825..11240)
                     /locus_tag="ACG85N_001616"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0224389.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11257..12573)
                     /locus_tag="ACG85N_001617"
     CDS             complement(11257..12573)
                     /locus_tag="ACG85N_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0224390.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12723..13421)
                     /locus_tag="ACG85N_001618"
     CDS             complement(12723..13421)
                     /locus_tag="ACG85N_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFQ0224391.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13710..14699)
                     /locus_tag="ACG85N_001619"
     CDS             complement(13710..14699)
                     /locus_tag="ACG85N_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0224392.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14779..15612)
                     /gene="msrA"
                     /locus_tag="ACG85N_001620"
     CDS             complement(14779..15612)
                     /gene="msrA"
                     /locus_tag="ACG85N_001620"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0224393.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
BASE COUNT         4896 a         3187 c         2693 g         4954 t
ORIGIN      
        1 ctttttcaga aattaactga tgatttcaaa aagaaaacat gtcataatat ttcccatatt
       61 gtgcaaaaaa atacaaggcg gaggctctac tatgattgaa gaatatattg agaaaaatat
      121 tttaagacag ctgtttttat gtgggcaatt ctatgtaaat aaagaagtca atctagaaaa
      181 gctctccaat ctcctgcatg tttgcaaaac cactttactc aatgatatca ataacataaa
      241 aaaagaattt gaagaacaga tcgcttatac acatcgggaa aaggaccgtt acactttata
      301 tttttcagaa catatacctc gatgcaagat catgcagcaa ttgtctcaaa attcgttgtt
      361 tttaaaaact tgtctgcttt atttggaaga agatgaacct gattatcttc aattaactga
      421 atgtgaattc atctctgtca gcaaagctta ttcattaaaa aaacaagtat tagcttattt
      481 taatgactgc gggatcgaaa tcgaccgcta ctcccctcga ttcactgaaa tggaaagacg
      541 tctgctgctt cttaatgtgt cgtatcgttt aggcggtttt aattcttggg agctacctga
      601 atcgtttttt gaacgtgccg atcgtttcat tgaatccgta acggaaaata gtggcagatt
      661 ttatgataaa gaaaataaag aaatcttatc gattggtttt gctatcagtt tcctacgcca
      721 gcaagtgtgt gctgtgacaa tagattcaaa atttatcgaa gaaatcaaaa aacgtcctat
      781 ttacaattat gtcgaatcag catgggaaaa cactgatttc caaacctatt ataaaaaaga
      841 agaattcgcc tttattttga cactattcaa tctttgtaat tatggtttcc attcttatca
      901 gttgattgcg gaagactttc aacagctaca ccaagtgttt attgacaata ctcccgaaat
      961 aaaagaacta gttgctacct ttgaatctca cttcaatcaa gaattattcg gaaaccaacc
     1021 gttcgaacgt gcgctgattc atctgatgcg ttcggcttgg gataactatc aattattcat
     1081 gccagaaaaa ttttatcttt taaatgagga acagacaaat ttatataaag aagtccagac
     1141 aattttttcc agctggtcca gccagttacc ttatgatcta cggttgaatc ctaattgcat
     1201 gcgggctttt gtgatcgaat tgagcggaat tctccgtctg acaaaagaac atctgactat
     1261 ctatatcgtc acaaattcgg atgttcatta tctgatttat cgggaagcct tggaagctgt
     1321 aactactttc gattttcaag ttgcaccaac aatttattca agtatatctg atataaagaa
     1381 gtatgcgcag cagtcctcaa accgggtatt atgtgaacgt acattatata caccagatgc
     1441 ggtccaatat gaaaatatta ttcctatttc gatcaatacg atcgatcgag ctatcatttc
     1501 agctgtgcaa aacaaataag gggctgtgac aaaaatcatg tcacagcctc ttatttgttg
     1561 aagatgccca tttctgtaac aatctcttga tacacgtcag gctcacaacc tcacttattt
     1621 tggacatcta cgggtccgat ctctttattt aaaaaataag aaatgatggt tcggataact
     1681 accaacgccg ctagttggaa aatatcctgc aaggtaggtt tgataatcga ttcgatgatc
     1741 ccagctgaaa caagaatttc caaacttaga agtatatagc ctcccaacat ctttttaata
     1801 gcattgttct cgctgactcg gtcgctttgc acttttgtat ggacaaataa atatttcaaa
     1861 aaatccttga ttgatagaca aacaccccaa accaataaac agatagcaaa gatatcaagt
     1921 cccagtacaa gtttttcgaa atatggaaga agaaactgca tcaactctgt cgtcatccta
     1981 cctctcctct tctttgtttt tctttgtctt ctatttcctg attcaagaaa taagaaatga
     2041 ctgttcgaat gataataata agtgcaagct tcaaaatatc tgtccaagtc ggcttgataa
     2101 ctgactcgat gatatcagca acgactagta cttccaagct taacaaaata taacttgcta
     2161 gcagttttct tgtatctgca ttacgagtat tacgttctgc atagtttttc ttgacatgag
     2221 accaatcaaa aagtttgaat agaacaagaa cgatcccgac aattaagata ccgatagaac
     2281 aaatatttaa aatagcaatg atccattcta gaaattcaat taaatttgag cgaaacatat
     2341 acgctaccta ctttctttta atttgttcct attcttttat tgtaaaagaa agttaccttg
     2401 ttcgtctatt taaaagttgt tctaatgata acggttcgct taaatttttc tttttcactc
     2461 tcccattttt ctccctccat ccataaatcg tggaagtatc caaaacaacg tagcaagtta
     2521 aaataaggct tagtccgtca tcagcttttc aaagaagcac tgtctcggtg ctttttttgt
     2581 acccaaattt ctaaaaatag aaccatacac ctcctcaaat cttttcttct tacctaccct
     2641 atttttattt acaggaaaat cagacacttc ttcttttttg tcaaaatttc ctttttcttc
     2701 tcgtcctatc atttggcatt ctaataaaaa cgaactatcc ttggatatag aaagagaaaa
     2761 caaggaggcg tttttcatga gtaaaaaatt aatcaaagtc ggcatcggtt taggtttgct
     2821 tgcccttggc gctgcatatc taggaaaaaa gacaggacta ttcgaagatg acagtcattt
     2881 atatgatgaa ttcgaatcaa tctagcatcc tatttagttg aatcactacg gacatacttg
     2941 gaagacattg gaggaatcac aatgagaaaa agtaaagcaa taatgattgg tgccggtatt
     3001 gccaatatgg cagcggctgt ttatttgatt caagaaggca aatggaaagg ggatcagatc
     3061 accttttatt cattggatga tcacggttca aatgatggtg caccgaccga cactgtagtg
     3121 gatgaatatt ggaataaaaa ccatccatta gaaaaccaaa aaggctatat tgctcgaggc
     3181 ggtcgcatgc ttaattaccg tacctatgta gatttgatgg atctgttaag tcgaatacct
     3241 tctgttactg agccagggat gactgctgaa gaagatacga gagattttga tgcaaaacac
     3301 cgaacctttg acaaagcacg tttgatggaa ggtggcaaag gaatcattga tggcggaaaa
     3361 ctgggattca ataataaaga ccgtattctt ctgactaaac tgattgctat gcctgattca
     3421 gaagaagaaa aactagacaa tatcactatc gaagaatatt tcaaagatga tccacacttt
     3481 tttgaaacaa acttctggtt tatgtgggaa acaacatttg ctttcagaac acgtagttct
     3541 gctcaagaat tgcgtcgcta tatgcatcaa atgatttatg aattcactca aatcgaacat
     3601 ctagtgggcg tcaatcgaac acgttacaat caatacgaaa gtattatgct tcctttgatc
     3661 gagtacttga aagaacaagg atgcaaaatc attttgaacc gccgtgtcac tgactgggaa
     3721 ttcaaagaaa caccgatgca agatgaaatc actgtgactg gtttgaaaat taccaataca
     3781 ctgactcaag aagaagaata cgttgtggtc gatgaagata cagcagttat tttcactaat
     3841 gggtccatta cagattcagc tactttagga gattacgaaa caccagctgt agaaaatatg
     3901 gattatggtg cagcttctag tctctggaaa aaagccagtg aacgtttcta caacttggga
     3961 aatcctgaca aattctttgc tgatcgagat gcaagcgaat gggtcagctt taccctgacg
     4021 acgaaaaacc atttgttgtt gaatgaaatc acccgtatca cgactcaaga accaggaaat
     4081 gccttgaatt catttatttc aaccacaccg atcacgccgt taggccaaaa agatgtcaat
     4141 atgtctattg tcgtgcatca tcaaccgcat tttacgtctc agaaaccaaa tgaaacagtt
     4201 atttggggtt atttccttta tccaagacga cgtggcgaat ttgtggataa acagtatatt
     4261 aaaatgaatg gaaaagaaat gctagaagaa ctgattggac aactttcaaa agtagatcct
     4321 ggaccagtga atattcgtga aaaagaaaca gaaattttcg acagcgtaat aaataatatc
     4381 cctgtctata tgccttatgc ttccgcgttg ttcaataatc gtgccaaaag cgatcgacca
     4441 aaagtcattc ctaaacattc gaccaatctg gcctttacag gagaatttgt cgagcagcct
     4501 tatcagatga tctttactga acaaagtgct gtacgttctg gagaaatcgc tgctttccat
     4561 tttgcgggta ttcctatgtc ccgtcttgtc aaaacaccgc gttacgacaa agatattcct
     4621 actctagctc gtgccgcaaa aaaaatgttt gaataatcaa gaggttgtga aaaagctgcc
     4681 ctgcatcaag taataagaac aaccaggcta aaacagcttc ttatgttttt gatttggttg
     4741 gacttattac cgcagatgca agcttttgaa caccgtttac caagaggtta tgaaaaagct
     4801 gccctgcatc aagtaataag agcagccaag ctaaaacagc ttcttatgtt tttgacttgg
     4861 ttagacttat taccgcagat gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa
     4921 ggcgtttagt ttcgagtatg aagaaaaatt ttgtgaaaat ggttcctcac gtttttgcat
     4981 aaatttgact taataccgag aaactgatcc cttctgtcat aacctctttt tatttatgtc
     5041 tgtttctttt tgaaaacaac tattttttca acgtttactc tcttgccaat ccaacatcat
     5101 ctaacggata caactatttc aatgattacc attacactaa aaaatcggaa acacacctct
     5161 ccgaacacaa atacataaga ctatagaatg tttcatttaa gaaaaaaagc gtttcattat
     5221 caaaaaggta agatctcctt taaaatgaaa ttatctgtag tactaataat gcagacaatt
     5281 ttatgaatct gaaaggagca tctccaatga aagcagtcac ttggcaagga aaacagaaaa
     5341 tggaaatcag aaacgtagat gatccaaaaa tattagaacc aacagatgca atcatccgta
     5401 tcacagcaac agccatttgc ggctccgatc tgcatcttta ccatcatggt gaagcagttt
     5461 tggaaaaagg gtatgttgta ggacacgaac caatgggaat cgttgaagaa gtgggatctc
     5521 aagttcaaac attgaaaaaa gggcaacgtg tggttatccc ttttaatatt agttgtggtc
     5581 attgtcattt ctgcctgaat cacatggaaa gccaatgtga tgaatcaaat ccagatcagt
     5641 tatatggcgg gcttttcgga tttggcaagt tgaacggtaa tcattggggt ggtcaagcgg
     5701 aatatctaag agttccttat gcagactcta cttcatttgt tgtaccagat aatgatcttc
     5761 ctgatgaaaa agttttattt ctatcagata ttttgcccac agcttattgg agcgttgaaa
     5821 acgcaggagt aaaaaaaggc gatacagttg tgattctagg ttctggtcca gtcggtctat
     5881 tcgctcaaaa attcgctgtt atggctggag cagagcgcgt aatcgcagta gatccagtac
     5941 agcaccgtct agataaagcg gctaattaca atggggttga aacttatttc ttagaagaca
     6001 cggcacaagc tggaacagaa ttgtatgaac tggctaaagg tggcgcagat gtcattatcg
     6061 attgtgtagg aatggacgga ttagagcctg tcaaagaaaa agcgaaaaat ttagtaagct
     6121 tacaaagcgg cacaatctct ccattacaga tggcatcgca agctgtcaaa aaatttggaa
     6181 cagtccaagt aactggtgtt tatatgactc cagcttcttc ttacccgctg caagaatttt
     6241 ttatgcggga tatcgctgtc aagcatggac aggcaccaat tatccatcta atgccaaaaa
     6301 tttacgcaat gattgcagaa ggactattcg atcctacaca aatcattact cattcgctgc
     6361 cactatcaga agcagcacaa gcatatgaga tctttgacaa aaaagaagat aaaaatataa
     6421 aagttgtttt gaaaccttga attcagtaaa aaatacaggt gattttcttt atgcccaaaa
     6481 tcaaacgaaa tacccatgag atcgaaatag gaaaagaaga agatatcgaa atacgaggac
     6541 gtcgcttccg cttctatttc caaaatcgtt acacattgat ctcactggct gtcgatttat
     6601 taaccgggat tttctatatt tttggaagcc ttgcttcctt aactaaaatc cccgatcgat
     6661 atggcaccta tttttatttg gctggtgcta ttttcttgac gattcgtcct attctcagaa
     6721 tcgtaaggaa catttttatt tacgacgaaa aaaaagcaat gaagagaaca agcaaaaaaa
     6781 aagaggcatg agaatttttc aaatctcatg cctctttttt caccctatcg tatcactttg
     6841 atacagacga gcggtcgtcc cactttcttc cttgaccatt gtcaaaaatg tccactcgta
     6901 tcgttcataa aacgtggtat gatctgtaat taagtaaagt ttttcgtacc caagttgatg
     6961 catttctttt ctgatttccg ctaatatccg cttggctatc ccctgcttac gcttttccgg
     7021 ttcaacaaac agggcacaaa gatttggtgt cagatcaggg cggttatgga agtcgttttc
     7081 aatgacgccc gctcctccga cgatggtttc tttttcatct aagaccacat accattgagg
     7141 aatactttcc ttattagcga ttccagtacg aatactttcc tcatacacaa cagcaggaac
     7201 tccccatttt gatgaaaacc aattcgcgct ttgttttact aattctgggt gttctcttaa
     7261 tttgatccag ttatatgtaa ccaatgttta aacctccgac taaaataaaa cgtttacaaa
     7321 aattatacaa gtctttatga aaattagcaa tgacaacaat agtgatgtaa aaaaaaaaac
     7381 ggaaaacaat cacttgttct ccgttttttg attaatcaag catctaaagc ttcttttctt
     7441 ctttcttgga tcgtagctac cttgacgaac ggtagataga tcaaaataga aagaaccaaa
     7501 ttgacagcgg ctagtacacc acctgcaata ctttttgttg ccaatacacc gccaataatt
     7561 ggtggagtta cccatggagg cataacggta gctgctggca ctaaacctgt agaagtcgca
     7621 aagtaagcaa cactaactaa gaccattggt gtcaaaataa atggaatgaa catgatcggg
     7681 ttcaacacga taggtaaccc aaacatcgtt ggttcattaa tattgaagac acctggcgca
     7741 atcgacaagt ttgttacaac catatatggt ttgtttttac gtcctactaa ataaatagct
     7801 aacagtaaac ctaaagttgc acctgttcct cctaagttga caaagctgtc aaagaaaggt
     7861 ttgttcacga taaacggtgc gctatgtcca gctgcgattg cattaacgtt tgcttcaatg
     7921 gctggcgcat tgattgtttg catcaaagga tcgaccatat tcgctccgtg taaaccaaag
     7981 aaccataaga atggtgtgat aaatgcaagc agtaaggcag atggataaga gtttgcaagt
     8041 cccataaatg gttcttggac cactgtatag aaagaaccta cgatatcatc aacaccgaat
     8101 gcagcaaaaa ttgcagcaac taaagcaaac gcagagatag tgatcattgc tggtagtaat
     8161 gaagcaaatg cttttgcaac tgccggtggc acaccatcag gcattttgat gatcaatttt
     8221 tcatttccgc ttaaacgtac aaagatttct gttgaaataa tcccaacaat aatagcaata
     8281 aacaaaccgt ttgagctcat acctaatgct ccgcctaaag cgaagaaaga gcctaaagaa
     8341 atcacacctg cggaaacacc gtcttttcgg tagccgttag ctaaatgata agcaagcaag
     8401 aaagcaatca agacagaaag aatggcaaat gtaccgttcc aagcagcagc cccgaatcct
     8461 ttccatgatt caccaccaaa aattgagttc atcaattctt ggaacccggg aatcggtaag
     8521 ttattgatca ttactgctaa tgctcctaag atcattaacg gcatactcac cataaatgag
     8581 tcccgaatcg caactaaatg ccgttgtgca ccaatttttg atgctacagg tataaaatgt
     8641 ttctccataa atgcaacaaa tttatccata aatcttccct cttttcttta ctgtagatta
     8701 cgcgttcaat cgacgataca gatcaatgat ctcttttgct aaatctgtaa aagctataga
     8761 tgtcatcaga tgatcttgac tgtggaccgt caaaagcgtg acttcaacgt gatttccttg
     8821 tgcttcttgt gtcagcatct ctgtttgaga gtgatgtgct tggactaaag attcctctgc
     8881 atctttgatt ttttcatctg ctaaagtgaa atcacctttt ttcgcagcag caatcgcttc
     8941 cattgcatcg ctttttgcat ttccgccaaa catgatcaga cccatgatgg cttctagatt
     9001 tttttgatct tccatttatt gtatcctccg ataattttta ttatttgttt attagttttt
     9061 ctgcttggtc gagtactttt tcaccattca tcatgccata gtcagccata ttgattacat
     9121 ctaaaggaat gcctttaggt gctagctttt cagcaaattg attttgcata aagcgtactt
     9181 gcggtcctaa aagtaaaacg tctacatttt tagattctag ttgattatcc gcatctgaag
     9241 cagatacagc aaagatgtct gcttccattc cttttgcttc tgctgccttt tgcatttttg
     9301 tgactaggag acttgtactc attccagctg aacatacaag catgattgtt ttcttttcca
     9361 ttttaaacac gttcctttct atacttacac ttgtatataa gcaagtagcg tgccaacttt
     9421 aaaagcgctt taaaaagttg atatcatcac atttaaggtc tataccaagt tgcatacaca
     9481 ctaaaaaaga aagcgcacac taaacacaca aattaatttt tattaccttt tttcttttaa
     9541 cgtgcacaaa aaaagttgtg acacaaaact tgtcacaact tttttcaaat aaacggtatt
     9601 caaaagcttg catctgcggt aataagtcca accaagttaa aaacataaga tgttgttttt
     9661 actttgctat tcttattact tgatgcagga cagctttttc acaacctctc aataaacggt
     9721 gttcaatcag ctgaccctgg gtattagctc aaaacgctgg attcacaacc tcttttattt
     9781 caaataacca gtaaatacaa tgaacatcta caaaaaactt ctatttattt atagatttcg
     9841 tcaggcaaat ccaattctct cttatcaatc aaagtctgat accacgaagc agattttttc
     9901 aatgttctgc ttcgttcatc gttcaattcg attcgaacta atccatatcg attcttaaat
     9961 gcattcattg gtgacacaca gtcagtgaat gcccataaca tatagccttc gcaatttgcg
    10021 ccttcttctg ttgctttgat caagtaatgc aaatgctgtc cgataaattc gatacggtaa
    10081 tcatcttgca cgattccttt ctcatccata tactgttctt catattctct tcccataccg
    10141 ttttctgtta tcagccaagg gatattatga taattttctt ttagatacat ggctatatca
    10201 tacatgattt tcggatagat ttcccatccc ctggaattat tcattttctt cccaggtaaa
    10261 tcaaagactt cataatatcg ttcaggatgg aaaggtgtct cttcattcca ttgataacga
    10321 ggggcgcgaa cgcgtttagg ataatattga ttgattccaa ggaaatctac tgtatgatgc
    10381 tgaatcgttt ctaaatcctc tgttgcagga tcgaaataaa tcgattgttt ttgacacaac
    10441 tccaggaact cttctgtgta gtatcctttt accatcggat caaaaaatac tcgattataa
    10501 aacaaatcat acatataagc agctttttgg tcttctggag aattggatcg agcatagacc
    10561 atttcaggat ttaaaataca gccaatcctt ccaccataat ttccgctgcg gtaaatctct
    10621 actgctttag ccgttgctaa aactttatga taattccaca tcatccactt ttttgtgttt
    10681 tgttcatgcg gccagcgaac agcatccaaa tagcaacgcg tttggggaac gattggttcg
    10741 ttgaatgtaa accagttttt taccctatca ccgtaacgat caaaggcaat ttgagcatac
    10801 ttagcataaa aatcaactac ttttcttgaa gaccagccat catatttttc caaaagaaaa
    10861 gctgggactt cataatgttc caaacaaatc atcggttcta cgtctgcttc gatcaaagag
    10921 tcgatcattt cacttatatg ctgagcatat tcttcatcta ctatacagtt ctcataatca
    10981 gtaaaaaaac gagcccaatt gatagaagtc cgataatgtg tcagatggat ggatttcatc
    11041 agatccacat cctgctggta ttgttccata aaattagttg ctacggctgg gccataccca
    11101 ttatgccaaa caaaatgttc atttttatac cagctatcta taaacgaatc ctgtccttct
    11161 ttttttcctt tccaaccttc cgtttgccaa gcagaagatg cagcaccaag aataaaattt
    11221 tttggaatag taattttcat gatttcctcc tgttttttag atcccatcat tttccacacg
    11281 atttgctact tttacaaatg gcagatagat caaaatagaa acaataatac aaacaagttg
    11341 cgtaataact gcaccgatat ttcctgctgt tgccagataa gcattgacga tcggcggcat
    11401 tggccaaggg accattacaa ctgctttcgc cgcaaatcct aaagcggttg caaaataacc
    11461 aatcgttcca gtgataagag gcgttaagat aaacggaata gctaaaattg gattcagcat
    11521 gatcggcata ccaaaaataa ctggttcatt gatgttgaag atcccaggag caagggataa
    11581 cttggtgatt tgtttcatat cttcccgttt tccaactaac aatactgcaa tcagtaaacc
    11641 gatcgtgact ccagacccgc ccatactcat atacatatcc caaaatggca tagtaataat
    11701 gttcggtact tcttttccag cttcaaaagc ttccgtattt acagcaattg ccgctaaaag
    11761 caacggttcc cgtatcggct tcaccatttg atttccatgg ataccaatca cccaaaagac
    11821 ttgcgcaata aacatcaata gcaaaatacc cggtaaccct tgtacgacac cttctaatgg
    11881 gcgttgtacg acattgtaaa tgatatcata aaggtacatt cctgtaagtt ctttgatgat
    11941 aaatcccgca gccgcgatac ccgatacagt cagaatcgtc gggaataaag cagaaaaact
    12001 agcagaaaca ttcgttggca cggtatcagg cattctcagc tgtagtttct tctgtttcct
    12061 caaccagcaa taaagttcaa tagaaaggat ggagaccaac attcccaaaa ataacccttt
    12121 cgtatctgta tattgtttag ccaatacgtt ttctactata acagtctgat tttcattcac
    12181 taattcataa acagttggat taacagataa ataagacatc actgcgatga taccagggaa
    12241 tacaccatcc actttattca gcttggctaa ttcgatccct atcagaaaaa cagcatagat
    12301 ggtcaagaaa cttagtgtga catagctgat ggcagatgcg ataggcttta attcttctaa
    12361 aaaacgcaag aaacttatct gtgctaagcc attttccgag tcaaagacca ttgctgaaaa
    12421 caatgtgcca aaagcacccg taataattac cggaatcaat gcagcaaatg cgttttttat
    12481 caccatgata taccttaaat tattgattct cgttgccacg ttccctaaaa cattggctaa
    12541 tttatccata aaactcattc cagatcccct cattcctgct tcttctcctt gacgtcatct
    12601 ctttttgtct attagtcagc gcttacattt cgatgaacag aaactattta ctcctctttt
    12661 ttaccaaata taaaacaaaa attcatctga tgtatttatc ataaaataca tcagatgaac
    12721 tgtcaattag tttctttttc tcctattatt ttctgtaatt ctctccgatt tattgccata
    12781 tggacagtca ttgcatcaaa cgcagcaagt gcattccgcc tttcgatggc ctgaacgatc
    12841 tctgtatgaa tatcgatcgt ctcgattttc agatgctgga tatcataatt tttattaaac
    12901 aaagcaattg attcattaat gattgggata atatgcagca aggctacgtt tccgctagct
    12961 tctgcaatca tcgtatgaaa atcgatgtct aattgaacat gttcttgtgt gccttctcga
    13021 aaagaagttt caatcttttg cgttaatttt ttcatttgga cgatttgatt cttcgttgca
    13081 tattccgctg ccaaacctgc catcttaggc tccaataaat atctcagttc aaataagtct
    13141 cgtatcatcc gatcctgatc ttcaatcaaa gaaaaaccga gtgggtcatt ggcaatccct
    13201 cgcttatcac tgacaaatgt ccccgctcct tgccgaactt caaggatatt cctggaagct
    13261 agagcccgca cagcttctcg caccgtgctt cgccccacct ctaaataatc ggacaattca
    13321 tattcattcg gcagtttatc tcctacttgc aactgcattt cctttaaata tctaaggatc
    13381 ttgtcagtag ttacttctac tagggtttta ttttcttcca aaccacatac ctccttttca
    13441 tctgatgtat tttttcgtaa ttattataac acgtttgaat catctgatga ttttttgtat
    13501 acaaaattga ttaatccgtt catagacata caaacgaaga agatatttac cataaaaaag
    13561 actaggataa aattcactcc tagccttcga tcatttttat tttgaacgac aaaaaatatg
    13621 atgttcaata acagacgatc ttcattcgat tcttgatgtc atcttttgtt ttttttaata
    13681 tatggataaa aagtttgatt acatcgtttc tattctagaa tttggtcgat tctcaattct
    13741 tcttctggtg agaaatctaa attatccatc attcgaacac tttcttgtaa ctgacttaac
    13801 cgacttgctc cgaccagtac acttgtaaca gatttttgtc gcaaattcca tgcaagggcc
    13861 atttgcgcta atgattgtcc tcgtgatacg gcgattgttt gcagtgcttt gattttttcc
    13921 agagttgatc caacttgttc ttctgaaagg aatggtattt ccttacgatg tgctctagaa
    13981 tcttcaggaa taccatgtag ataacgattt gtcagcaacc cttgagcaag cggactgaac
    14041 gtgattgccc ctagcccttc ttcttctaat actggagata gatcgtcttc tagtagtgca
    14101 cgagaaaaca tattgtagcg catctgatga ataatgaacg gtgcttcctt tctttttaaa
    14161 atttcagtca ttttttttgt atcttctcca ttatagttgg atataccaat atataatgct
    14221 tttccttgtc ttacaagttg catcaaagct tctgctgttt cttccaaagg tgtatcaggg
    14281 tctgggcgat gatgatagaa gatgtcaaca taatctagcc ccattctttg gagactttgg
    14341 tcacaacttg aaatgatgga tttacgagag ccccattccc catatggccc ttcccacata
    14401 tgataacctg ctttgctaga aataatcagt tcatcacggt aggcttgaaa atcttctcta
    14461 aaaatacgcc caaagttttt ctctgcagct ccagcagggc ctccataatt attcgcaagg
    14521 tcgaaatggg taattcccat gtcgaatgcg cctcttaaaa tctctctttg attatggata
    14581 ggatcataat caccaaaatt ttgccataat ccgagagata aaatcggtaa tttcaaacct
    14641 gaatgaccgg atttacgata ttccattttt tcataacgtg cactatctgc aatatacaat
    14701 ttttctcctc ctcatcaaat ctttttaaaa cgctttcttt tttctatttt tagtttacta
    14761 cattttaaag aaaaggagtt aatcaaaatg attttttcgt atattttttt gccatttgtt
    14821 ttttactcgt tgatagatca gaaattgatt acgtgcttgc tggatttttt tataacgttt
    14881 cggttgtttt ttgtaaaatt gctgatgata gttttctgct ggccaaaacg taccggctgg
    14941 ttcgattttt gtgacgattg gttggtggaa agttcctgaa ctgtctagtt tctgttttga
    15001 ttgttccgct aattcttttt gtcgttcgac ttgataaaaa atcactggac gatattgctt
    15061 ccctctatct tgaaattgtc caaatgtatc tgtgggatca gtaatttgcc aatagattgt
    15121 cagcaactcg ctataactta tttctcttgt gtcataaatg atctccacag cttccacatg
    15181 acctgtgtaa ccaccactta cttgatcata atttggtttt tcaacatgtc cgcccgtgta
    15241 tcctgacaaa acagatagga tccctttttt cgattcaaac ggctcgacca tacaccagaa
    15301 acatccccct gcaaatactg cacgttcttg atagtcagca tcttgcatgg catgcttgga
    15361 aaagtcatat ttttgctcct tatcaaatcc ttccgtaatc aataaataaa agtccattac
    15421 atcaggcgtc agattcattc ggatagctaa tggacgtagt gttgcttcta gttttgataa
    15481 ctgttcttta acggatatat tttctcttat gtcattcttt gtctgaatca atacttttcg
    15541 ttcccaatct cgtgtattcg gattcaaaat cagattataa agtgtttgaa tgaatatttg
    15601 ttcctcatcc atagattctc ctcctttccg aagaagacaa gctagtgact ttagctgtaa
    15661 tttatcataa agtgaggaaa taagcgatta cttcatcccc ctacagattt ttcgtataaa
    15721 aaagagagaa
//
LOCUS       DAZKLA010000058        15449 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000058 DAZKLA010000000
VERSION     DAZKLA010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.059"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..686
                     /locus_tag="ACG85N_001621"
                     /pseudo
     CDS             78..686
                     /locus_tag="ACG85N_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            706..1734
                     /locus_tag="ACG85N_001622"
     CDS             706..1734
                     /locus_tag="ACG85N_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0224394.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1731..2489
                     /locus_tag="ACG85N_001623"
     CDS             1731..2489
                     /locus_tag="ACG85N_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0224395.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2575..3315)
                     /locus_tag="ACG85N_001624"
     CDS             complement(2575..3315)
                     /locus_tag="ACG85N_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0224396.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3376..4629
                     /locus_tag="ACG85N_001625"
     CDS             3376..4629
                     /locus_tag="ACG85N_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0224397.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4924..5178
                     /locus_tag="ACG85N_001626"
                     /pseudo
     CDS             4924..5178
                     /locus_tag="ACG85N_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5300..5626
                     /locus_tag="ACG85N_001627"
     CDS             5300..5626
                     /locus_tag="ACG85N_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0224398.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5694..6407
                     /locus_tag="ACG85N_001628"
     CDS             5694..6407
                     /locus_tag="ACG85N_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0224399.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6717..7569
                     /gene="licT"
                     /locus_tag="ACG85N_001629"
                     /pseudo
     CDS             6717..7569
                     /gene="licT"
                     /locus_tag="ACG85N_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            7690..9555
                     /locus_tag="ACG85N_001630"
     CDS             7690..9555
                     /locus_tag="ACG85N_001630"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0224400.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9569..11012
                     /locus_tag="ACG85N_001631"
                     /pseudo
     CDS             9569..11012
                     /locus_tag="ACG85N_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            11120..11821
                     /locus_tag="ACG85N_001632"
     CDS             11120..11821
                     /locus_tag="ACG85N_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0224401.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11947..12459
                     /locus_tag="ACG85N_001633"
                     /pseudo
     CDS             11947..12459
                     /locus_tag="ACG85N_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(12857..13066)
                     /locus_tag="ACG85N_001634"
     CDS             complement(12857..13066)
                     /locus_tag="ACG85N_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFQ0224402.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            13307..13882
                     /locus_tag="ACG85N_001635"
     CDS             13307..13882
                     /locus_tag="ACG85N_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFQ0224403.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            13882..14322
                     /locus_tag="ACG85N_001636"
     CDS             13882..14322
                     /locus_tag="ACG85N_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0224404.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            14338..15039
                     /locus_tag="ACG85N_001637"
     CDS             14338..15039
                     /locus_tag="ACG85N_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0224405.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            15211..>15449
                     /locus_tag="ACG85N_001638"
     CDS             15211..>15449
                     /locus_tag="ACG85N_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0224406.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMP"
BASE COUNT         5213 a         2562 c         3255 g         4419 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttaccca gaaaatcaac tttttgcagg agatgtatta gaaattgtat
      121 taaacgacca agcaggagaa gctgctgcta agggtgtgca gaacaagcca agtaccaacg
      181 atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat gccccgcctt
      241 ttgcacctgc tacgaagatt gtagtagaag gtggtctctc atttcttgcc ccttccaaac
      301 tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga agcatttggc caaatggata
      361 tacaggaaaa attatggatt tatgatcagc gagaaacaaa aagtccatgg atcttaacga
      421 taaaattatc tcagcctttc acaaccgatg caggattttc tatgcaggat gtcctatatt
      481 tcaagaaagc tgaggagtat caattagtga atgaagaaga actggaaatt gtcagaggac
      541 aaaattcaac gaatgtgcaa acagattatt cagaatattt aaataatggt cgggcgttta
      601 aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag atgacatgga
      661 cattagcaga tgcgccgaca gaataaagat caaggagaat gattgatgaa tcgaatatgg
      721 aataaaagtc atcgaaacaa ctgctcttta cttctgatcc ttctccttgg attcatcctt
      781 tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga attaccggct
      841 aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg gaaaacacag
      901 gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga gacattgatc
      961 cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa aagtaaattt
     1021 gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga aataactgtc
     1081 cctcctcaaa gtgcggtcac tgagacactt tcactgaaag ctccagaaaa agagctggct
     1141 ggtgtggtcg caggaggaat cacgtttcaa gaaaaaaaag agaacgtttc taacaaaagt
     1201 acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgattcg aaatagcgaa
     1261 aaagagtctt ccccaaattt gaaattaaaa aaaacttatg cggatcaaag cgatggaaga
     1321 aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg cttgactaca
     1381 tcggttgaaa tctacaaaaa agaaacaaaa gagccttatc tttcaataaa aaaagaaaaa
     1441 atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg aaaaaaatta
     1501 gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg ctggaatctc
     1561 cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa ggatattgat
     1621 ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg agtcattctt
     1681 attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa atgaaaaaaa
     1741 caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa agtgtttttg
     1801 ctgagaataa gagctcagcc actacagaag gaacgatttc tgtaacaaaa ccagatgccg
     1861 caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatccc gatacgccgt
     1921 ttacacctaa cgaaccaaat cagggaacat ccggattact gacgattgac caagctccag
     1981 atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct gcattacaaa
     2041 acggaacgga cagtacaggg acagcaacac aagtacagaa tttcgtacaa gtaacggata
     2101 aaagcggaat ttatgccggt tggaaactat cagtcaaacg aacagaattt attggtacaa
     2161 catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc gtgattcgaa
     2221 atagcgcaac taatagcagt ggaacgccat ctactatttc tggtactggc gctgaaggaa
     2281 ttgagatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat gaagggattg
     2341 gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca gtggaattat
     2401 ttgttcctgt atccaattat gccgtagaca actacgtttc aacattcaca tggtcgttaa
     2461 cagatgctcc tacagaaggg aaagcgtaaa atcaaatcaa tgacttcagg gagtggagcc
     2521 gattcaaggc caactccctg aaatctgcta tgcttacgtc tcagctctta ttcatcaact
     2581 cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga tctttcgacc
     2641 aacgagtaag gagtcaccat tgaaaaattc taacacataa tgagcagaat caattttctg
     2701 caattgcgat aagttgatac agtaagattt taatagtaaa aagtaagcat acttttccaa
     2761 ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag ttgttttgac
     2821 cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc gaggatacag
     2881 gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga tttgctgttc
     2941 tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc tgtcttttct
     3001 gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat tcgtataagc
     3061 ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg cgacatcaat
     3121 acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat tatcaatagt
     3181 acccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga tattctcgat
     3241 atgatgattg accaattctt tgatgatcca ttcgctgtga aactggttat cttctatgat
     3301 aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt tgaaacaaaa
     3361 caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac tttgatttta
     3421 gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag ctttggtttt
     3481 atagctttga tggtggtgac acttttattt cctatcccta aagtgagctt tccttaccta
     3541 gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt tatcagctgt
     3601 ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt ttttgatctg
     3661 atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact acttatcgca
     3721 tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct caacttgctt
     3781 tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag ctggggcgtg
     3841 atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct tctatctctt
     3901 ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat cagtttccaa
     3961 gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga agaatattac
     4021 catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt aaaaattact
     4081 cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tcttagaaga tcattctgca
     4141 gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc agctgtcagg
     4201 ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat ccaagtagtt
     4261 attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg ctgtttatct
     4321 atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat tacgatcaat
     4381 ctgctggaag aaaaagaggg attacgtttt agtatagcga acaaggcaaa tccgacgaat
     4441 ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg cgggttaggt
     4501 ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa aatcgaattt
     4561 tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa gagcaaatca
     4621 gcctactgac ttactgtcaa aggataagct tgaggaaagt ctacacggag atagagacaa
     4681 caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct tctgtttctt
     4741 ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag tacgaaagta
     4801 tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg tcaaaatgct
     4861 aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta ggcgtggaga
     4921 agcaagaaaa tccctacgat cacacaaaaa atgctgaccc agcagttaag agagttagaa
     4981 caagatggca ttgtccatcg aaaagtctat aaccaagtcc cgcctaaagt agagtactcg
     5041 ctcaccgaag aaggaaaatc attaagagag attctagtag cgatgtctgt ttggggagaa
     5101 gaacgtatcg aaaaacaaaa agcctcaggg gaagaagtca cattactgca tacacatggt
     5161 ggctacctaa attactgatt tctatgacat ttattttaaa taaaagaaaa caggagtttt
     5221 tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa gtaagaaatg
     5281 attggaagaa ggcaagcaaa tgaatagcat ggaaaaagtt aaccagttta gagatgaacg
     5341 taattggcga caatttcata acgaaaaaga tttggctatt tctatttctt tggaagcatc
     5401 tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa cacaattaga
     5461 acgaatcaaa gaagaactgg cggatgtatt gatctattca tacatgatgg cagacaacct
     5521 tcagctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt tgaaatatcc
     5581 tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttttagaaat gtgtcattcg
     5641 gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag aaaatgaaaa
     5701 aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg ttattagtag
     5761 gactagcttt gattttcaat gaacagatca aggattatct tgtcagagag acaggagata
     5821 aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat aaagatgtca
     5881 gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg tcgcagatga
     5941 gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca atcaatttac
     6001 cgattttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca ttatctccag
     6061 atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg aaccctgaat
     6121 tgttgtttac accgctggaa aatttagaga tgggtgccaa gatctatttg acagatttag
     6181 aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca gatacccaat
     6241 tactgaatga agtcgaggaa aaagaaattg tcacactaat tacttgtggc gacatggacg
     6301 ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacaccgata aaagatgcga
     6361 cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat actgggacat
     6421 aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa accaatttgc
     6481 tgaaaaaaag aggaacgcaa agaggtgcat ctctgatttg gttgttttct agatagactt
     6541 gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa ttcgatggta
     6601 tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca gaattcagct
     6661 aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga agcaaaatga
     6721 aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc ggggaagaag
     6781 tcgtttgtat gggaaaagga ctagcttttc aaaaaaaaga ttggggacga gatttcgcca
     6841 gaagcggtcc aaaaagaatt tattttaaga gattcttttg ctaaaaacca attccagcag
     6901 ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt cgaactggct
     6961 gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac cgatcatctt
     7021 cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt attatttgaa
     7081 acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt gttcgtgaaa
     7141 caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt tcattttgtg
     7201 aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac aatggtaaga
     7261 gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga atctttgaat
     7321 tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa aggggaagga
     7381 tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc aaaagcgttt
     7441 cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga aatggggatc
     7501 gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa aaaaagcatc
     7561 caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa taggttcacg
     7621 aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa aagaaagaag
     7681 gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt aggaggacaa
     7741 aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt aaaagatgaa
     7801 tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt cgtacaaagt
     7861 ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca atcgatcgtg
     7921 gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa tattttgaat
     7981 cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat ggcagccgcc
     8041 ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac agctgatagc
     8101 ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt acctgtcatg
     8161 cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt agcaattgct
     8221 atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc tatcagtttc
     8281 tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat accaatcatt
     8341 ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat tccgcaattt
     8401 ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt aacatttctt
     8461 gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt caactcgatt
     8521 tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca agtattcgtg
     8581 atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga acaatatggg
     8641 tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg ggctgcttta
     8701 gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc ttctacagtg
     8761 acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc tttgaagaaa
     8821 ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg attttccgga
     8881 gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt cattaatacg
     8941 gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat tgcttttgtt
     9001 cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga caatcaatta
     9061 gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac cttaaaaagt
     9121 cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt ttcttctggc
     9181 gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc acctgcagac
     9241 ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac gactgacgga
     9301 gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa tggctttgag
     9361 atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg ttttgacatc
     9421 gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac gaatactgat
     9481 gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga agatgttctg
     9541 gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa aatttttatg
     9601 gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac ggaaaaggac
     9661 tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc ggcagtgaag
     9721 aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga ggaatcgatc
     9781 attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc aaatgttatc
     9841 gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact cctaacgaag
     9901 cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat attgaacctg
     9961 tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac ggtggctgga
    10021 aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta gagagataca
    10081 gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt cactttccag
    10141 ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat attttccaag
    10201 catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac gaattgcgtt
    10261 cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc gatgccaatc
    10321 cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc tgcacggatg
    10381 tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa tatggagtcg
    10441 atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa tatccagtgg
    10501 actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat ccacaagcag
    10561 caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat
    10621 ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta tacaatcgtt
    10681 accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat atggaagagg
    10741 atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc gaagcgatgg
    10801 ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga tgtatcgatc
    10861 ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat gtggatctag
    10921 acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat tggtataaag
    10981 aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa aaagaacaca
    11041 cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt ttagtaatag
    11101 taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag tcgcagataa
    11161 aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc cgacagaaag
    11221 tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct
    11281 ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc taagcaatcg
    11341 tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga actcccgata
    11401 tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg aagatccttt
    11461 gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt tgaacaatca
    11521 gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca gcagagaaga
    11581 atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta ttgactcatt
    11641 ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc tgaaaacaga
    11701 cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg aaatgattga
    11761 atattcggag gcggtctacc atacggagct gcatccttac catattgatt atgaaacata
    11821 aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    11881 aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca cggctgtttc
    11941 tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg aatctatgaa
    12001 ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac gatcgcttat
    12061 ttcttaggga aaatcattcg taaaaatata ctcaatcgtc aagaaatcga tacattgaag
    12121 accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat cgtattgaac
    12181 gacttggcac gcaatgtcat agcaacggga gtcggtggga cagtaggtgg cggattaagc
    12241 atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt cgctacgatg
    12301 accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac aggaataacc
    12361 attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga agaaaaagag
    12421 gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa tggattacaa
    12481 gaaaaaagta caatagatgt accttgagac aggaatggta tgaaagaatc agagttagaa
    12541 agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag catcggcacc
    12601 ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaag ggtctggtat
    12661 accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa aaaggaaaga
    12721 aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt acacgttcga
    12781 gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca ctgaattttc
    12841 ttccagcagt gacgattcaa gtgatatttt tacaaatcca gctatttatc gttaacaaaa
    12901 agatttttag gagaagctga tgaacaaaaa tgatcaaatt ttgatcgttt agttcgtcgg
    12961 cttttttatt tagagaaaca tcatttatca gtgaaacata acacgagaac aaaatgattt
    13021 tttcgttaat aaaagaaaca agtatttgtt gtttaaaata atacacgtta ttggcatata
    13081 ggacgaaatc ccagtatctt ctataagtaa gtcatttcat atcgatgaaa acacgttatt
    13141 tatttttgtt ttatctataa aatggctgaa aaatgatttt tttctaaata ctatatatag
    13201 tggcgtctat cctatatacc actaaaaaac tacaaaatct agtcttttat tttaaaaaaa
    13261 cacgtgtaga atgaagatag acttttgagt tagggagtgt ttttttatga aacttactgt
    13321 tttttgcggt tcccgttttg gtaataaaga aagttacaaa tttattgcgc aaacgttagg
    13381 aaaatacatg gcacaagaag aaatcgaact ggtttacggt ggttcatact ccgggattat
    13441 gggagtgatt agtaaaacag tactggaaaa taacggaaaa gtcacaggta tctatccgaa
    13501 tgggcttttt gaaactgaat tgcctggaaa agatgttact acatttattc ctacagaaac
    13561 gatcgatgaa agaaaggtat tattatttga aaagggagat gctgtcctcg ttttccctgg
    13621 aggtctaggt acattggagg aattctcaca acttctttct tggatagcaa tcggtctgac
    13681 gcccgacaag ccaatcggaa tattgaatat tggcggttat tacagcggct tgcaacaatt
    13741 attagaaact tttgcaaaag aggattttat ggataaaaaa tggttggatc aagtaatctt
    13801 ttccaacaat ccattggaac tggtcaatga attacgagca gtatctatta aaaatcagtt
    13861 attattgaag gaggcaaaat aatgagtcaa atatatttag caggaccatt tttttcagag
    13921 gagcaaatcg atcgagtaag tcgtatcgaa aaagcgttgg aggaaaacaa aacagtcaca
    13981 agtttctact ctccacgtca tcaccaagaa agcaattatg aactattcag cgctggatgg
    14041 gcgcaggaag tatacgaaaa agatatggaa gaactgacaa atgcagaatt tgttgttgct
    14101 atccttgact ttgagcatca aacaatcgat ccaggaacgg cttacgaatt aggtgtggca
    14161 actatgttga aaaaaccgat gatcattgta caagaagaga cggtacctac gaacttgatg
    14221 attacacaaa gtctccatac gtatttaaaa tcagaccaag ctgtacgtga atatgatttt
    14281 gaaacattgc ctgttgaaac ttacgtaggc gagtacttat agaagaggaa gggttgtatg
    14341 tcagtgattt tgatttcagg aacgattggt gcaggtaaga gcagtttgac ggatatgctt
    14401 gcaaaagaga ttgattctaa accattttat gaaaatgtcg aagataacga agtattgccg
    14461 ctcttttatt caaatcctga gcagtacgct tttttattgc aaatcttctt tttgaacaag
    14521 cgatttttag caatgaaaaa tgctttagtg aacgatgaca atgtgctaga ccgatccatc
    14581 tatgaagata gcctgttgtt ccatctaaac gctgatttag gtcgggtaac ggatatcgaa
    14641 gtacagcagt acgatagctt attagatacg atgctaaacg agttagatga tgttgctcca
    14701 aaaaaacggc ctgatttgat ggtccatatc aaagtttctt tggacacgat gctagaacgt
    14761 atcaaaaaac gtgggcgtga ctatgaacag ctagaaagcg atgagacgtt atatacctat
    14821 tatgaaacgt tgaacacccg ctataatcaa tggtatgaag attttgatgt atgtcctaaa
    14881 atacaagtgg atggggataa attcgatttt gtagaaaatc cagaagatgc aaaaaaggtg
    14941 atccgtcaga tccaagaaaa attagctgaa ttggaaggaa agcctgctgg aaaatatttt
    15001 acggataacc agcatgcaaa aggtcaagaa ttaggctaaa aataaaaaac caaacgacgg
    15061 gaaagaaaaa ttacggctct ataatttatg ggactgatga atcagaattg attcttcagc
    15121 cccattttta ttttaggtat taaaaaacat atcgttctct agtatgatta aatcacgaca
    15181 aaataaacaa ctgagaggac gatatgctat atgaatgatt ctatcaaaaa aatgctgcga
    15241 ttaatagaga aagatttgat gattacagag gtctcttacg agacctttca gaagaaaaag
    15301 acgttgatcg tcgatgctgt tttctcacct gctccccata cctgtagaaa ttgtggttct
    15361 actgtggtag atggaaacgg gaaagtaatt gtagtgaaaa atgggaagaa ggaaacgatt
    15421 gtccgctttg aacaatacaa tcacatgcc
//
LOCUS       DAZKLA010000059        15255 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000059 DAZKLA010000000
VERSION     DAZKLA010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.060"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..1021
                     /locus_tag="ACG85N_001639"
     CDS             2..1021
                     /locus_tag="ACG85N_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0224407.1"
                     /translation="MSIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDHQLIT
                     GKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGWLFFF
                     TSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVVIGMI
                     ISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSV
                     FLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSMTNIL
                     LFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTV
                     RRLKR"
     gene            1018..1785
                     /locus_tag="ACG85N_001640"
     CDS             1018..1785
                     /locus_tag="ACG85N_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0224408.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            1885..3453
                     /gene="liaX"
                     /locus_tag="ACG85N_001641"
     CDS             1885..3453
                     /gene="liaX"
                     /locus_tag="ACG85N_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFQ0224409.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3750..4070
                     /locus_tag="ACG85N_001642"
     CDS             3750..4070
                     /locus_tag="ACG85N_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFQ0224410.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4070..4420
                     /locus_tag="ACG85N_001643"
     CDS             4070..4420
                     /locus_tag="ACG85N_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFQ0224411.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4587..5525
                     /gene="hprK"
                     /locus_tag="ACG85N_001644"
     CDS             4587..5525
                     /gene="hprK"
                     /locus_tag="ACG85N_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFQ0224412.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5540..6370
                     /gene="lgt"
                     /locus_tag="ACG85N_001645"
     CDS             5540..6370
                     /gene="lgt"
                     /locus_tag="ACG85N_001645"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFQ0224413.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6414..7439
                     /locus_tag="ACG85N_001646"
     CDS             6414..7439
                     /locus_tag="ACG85N_001646"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0224414.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7454..8392
                     /gene="galU"
                     /locus_tag="ACG85N_001647"
     CDS             7454..8392
                     /gene="galU"
                     /locus_tag="ACG85N_001647"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0224415.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8517..8957
                     /locus_tag="ACG85N_001648"
     CDS             8517..8957
                     /locus_tag="ACG85N_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFQ0224416.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8960..9562
                     /locus_tag="ACG85N_001649"
     CDS             8960..9562
                     /locus_tag="ACG85N_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0224417.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9625..10728)
                     /locus_tag="ACG85N_001650"
     CDS             complement(9625..10728)
                     /locus_tag="ACG85N_001650"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFQ0224418.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10938..11939
                     /gene="ccpA"
                     /locus_tag="ACG85N_001651"
     CDS             10938..11939
                     /gene="ccpA"
                     /locus_tag="ACG85N_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFQ0224419.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            12294..12689
                     /locus_tag="ACG85N_001652"
     CDS             12294..12689
                     /locus_tag="ACG85N_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224420.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            12689..12940
                     /locus_tag="ACG85N_001653"
     CDS             12689..12940
                     /locus_tag="ACG85N_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224421.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            12983..13378
                     /locus_tag="ACG85N_001654"
     CDS             12983..13378
                     /locus_tag="ACG85N_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224422.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            13375..14631
                     /locus_tag="ACG85N_001655"
     CDS             13375..14631
                     /locus_tag="ACG85N_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HFQ0224423.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            14643..14966
                     /locus_tag="ACG85N_001656"
     CDS             14643..14966
                     /locus_tag="ACG85N_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224424.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            14959..15225
                     /locus_tag="ACG85N_001657"
     CDS             14959..15225
                     /locus_tag="ACG85N_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224425.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         5242 a         2580 c         3225 g         4208 t
ORIGIN      
        1 aattagcatc atcactatat tttttatttg tgtgcttact tcatgggtta ttcagcatca
       61 agccgcttta tatgaagtac ctgtcatcga gatagaaaaa gtacgaataa aagaggatca
      121 tcaattgatt acaggaaaac tgatgaactc ggatcaaaaa ggagaatcga tttatttatc
      181 ggttccttat caaaaaaatc aaatagaaac agcaaatata aaagcgagac aaatgtatct
      241 agtccaaatg catggagatg aatggcagct agtagcaaaa aaaaatgatg gctggctttt
      301 cttcttcaca agtatcttca ttggcgccat gttgcttatt ggcggaaaac agggtctatt
      361 tgctttagga agtctcatca tcaatatcgt tttactttta cttttgttgc tcatctttac
      421 taaaacagaa gggatttctc tattaaataa atctatcctg ttcgtcgtta tagggatgat
      481 tattagcata ttggctttag atggatggaa tagaagcagt ataatcaaaa tttcagctgc
      541 agttacgagt gtttttttag cattttttat ttgtcttttg acaatgaatc attggaaaga
      601 ccaaggactg cgctatgagg aactgaatta tctgacacga ccgtatcgtt ctgtttttct
      661 ttctagcgta ctgattggaa cagctggagg aacactcgat accgttatca cagtcattgc
      721 gacattagaa gaattgacta agcgacaacc agaaataaca gcgaaacagt tatggctttc
      781 aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc aatatccttt tgttcgtcta
      841 tatcagtggg tcagttccat cgcttctttt atatttagga aatcaatggt ctttcaaaga
      901 aacgatagaa cagcatttat cattggaatt tttacgcgtc attgctggca gcttaggaat
      961 agtattatcg atccccatct cggtcctctt ttttctgact gtaaggaggc tgaaacgatg
     1021 aatgtactta ttatacttgt tgggatttta gcaatcagcg tgctacttat tggcggtacg
     1081 caaggtatgt atattctttt aggcttattc atcaatttag ggatcttttt cctgttgtta
     1141 ttcggttatc atcagaagtg gccgatactg gcattgtcgg tcataggatt tttattgatt
     1201 gctgtggtta tcttattttt tatcaacggt tataatctaa aaatgcgtgc agcttttgtg
     1261 agtatactga tctttttatt ttgtttccta ctattgcttc ctatcactga ttttttagcg
     1321 atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa gactttagcg
     1381 attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta
     1441 gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta
     1501 tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca
     1561 gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt
     1621 gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg
     1681 atggctattc ttaccacatt gtccgcacta attgttcttc ctttaacctg cgtgactaca
     1741 gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag
     1801 tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca
     1861 attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa
     1921 aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa
     1981 gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat
     2041 aaggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt
     2101 gatccaaagg aaagagaaag acaagatcaa gaaaacttag aaaagatctt agatgaacta
     2161 gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag taaatgctca attcgctgac
     2221 aataaggcag cacgcaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa
     2281 ttaggcgagt tgacagaaga agaattagca cagcgtcagg cgttagaggc agaaatcaaa
     2341 gagttagaag ctagtggcga tacactgtta gaagaacaat tgaaattaga agcagaatta
     2401 aaagatatcc gtaagaatca atggtctgag aaaaaagaaa cgtttactga taagtttgaa
     2461 ttgccagatg attggaaaga tcaagcaaca gatacactga atcaagtagg cgaaaaaatg
     2521 tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa
     2581 acagtgaatg acaacatgga atggaaagat gtcagcttac gtgtccctgg gattgcgaca
     2641 acgaaatttg agcatgaatt ttactatgaa gctcctgcag caagtatcct tgatatcaag
     2701 gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa agttgaagct
     2761 aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc
     2821 cagattgagg ttaatgaaga tcatatctcc ttccaaattc caaataaacg tgtgcgtgca
     2881 gaccttgttt tctacttacc taaacgagta tacgaccacg cagctattaa acttctaaat
     2941 ggcaacatca tgattgaaac attggaagct aaagatattt atacaaaatc tacgaatggt
     3001 aacatcatcg tggaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat
     3061 atcgatattc gaaacgggaa catcttagat tctattatcg aaacagtaaa tggtacagtg
     3121 acatttggtg cgacagcaga aaatctttct gtgtctttag taaatggtga tgtacgtctt
     3181 acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa
     3241 gtcgctttac cagatggcat cggtttggaa ggacatgcta aaacaagtct tggaagtatc
     3301 aatagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg
     3361 ttgcaattcc gtcgtgtttc agatgatgag atcgcgcaag tgcaactttc aacgacaaca
     3421 ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct
     3481 tgagcaataa gaagcgattt tgtaaaatag cttcccctat tttacaaaat tgtgccttat
     3541 tgccgatagt ctatctcttg aacaccgttt accaagagat tatgaacctg ccaattatcg
     3601 ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg
     3661 tgaccgagaa ttttgcagaa agacaccgat taagaagaag gttgtcacaa cgattttctt
     3721 catttaaaat agtagaacag aggtgagaca tgatgaaaaa aaagttgaca aaatctaata
     3781 aaaatgttgt tttgacaggg actttagcag gaataggaga atacatcggg attgatccga
     3841 ctgttatccg tgtgctttat gtattcctga gcttagttgc ttttggaagt ccaattattt
     3901 tatacatttt attagctttg attatcccat ctgcgcgttc tgcacgaaca aactatggac
     3961 ataacaatgc atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa
     4021 aacgcaaaga agcggaaaaa atcgatgatg acgaatggag tgacttttaa tgtcgtactt
     4081 tcaacgtatt ttagttaata cgttaacatt catatcattg gctgttcttt ttcctgcgat
     4141 ggtccatgtg agcagtattt ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa
     4201 tatgtttgtc aaaccgattt tgacgatact gtccctgccc tttacattag tcacattggg
     4261 attgtttacg tttgttatca atgcagcgat tcttaaaatg acctcctttt ttgtgggagc
     4321 agaacatttt agtttttctt cattcggtgc agcgttgtta gtagctatct tgatgtcgct
     4381 tgtcaatgta attgtcagcg aacataacag tagaaaataa aagaaagctg tgccgaacta
     4441 tcggcgcagt ttttttattt agaatcagga atgcgttttc tattacattg gtaattaaat
     4501 aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat gatagagaac
     4561 actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc caattagtga
     4621 aaaaattatc tttggaaatc gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa
     4681 ctggggacat ctcacgccca ggactagagt tgactggtta ttttaattat tattcccata
     4741 atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg atcccagaag
     4801 agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt attatttcaa
     4861 gaggattacc agctccagaa gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac
     4921 tgcgttctcc catctcaact tctcgtttat taggagaatt atccagttat ttagatggtc
     4981 gccttgcccc tcgaacaagt gtccacggtg ttttagtgga tgtttacgga ttaggtgtac
     5041 tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg
     5101 gccatcgatt gatcgcagac gatcgagtag atgtttatca gcaagatgaa ttaacggtga
     5161 tcggagaacc gcctaaaatc ctggaacatc tgattgaaat ccgaggaatc gggatcattg
     5221 atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg
     5281 tttatctgga agcatgggca aaagataaga aatacgaccg tctgggctct gaagacacga
     5341 ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa acaggacgaa
     5401 acgttgcaat catcattgaa gtagccgcga tgaatttccg tgcacgtaca atgggctttg
     5461 acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg
     5521 actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg agcttgtagg
     5581 gattcctatc tactggtatg cattgatcat cgtatcaggt atcattatcg ctatgtggct
     5641 tagcagcaga gaggctgttc gggtaggatt aaaagcagat gatgtgacag attttatgtt
     5701 gtggggattg cctatctcca ttattggcgc tcgcttatat tatgttttat ttgatcttcc
     5761 ccagtatata gctgatccaa tccaaatatt caatatccgt tcaggaggct tagctattta
     5821 tggcggactg attgcaggcg cgatcacgct gtattttttt actaaatatc attttatttc
     5881 tttgtggact tttttagata tcgctgcacc aagtgtatta ttagcacagg caatcggtcg
     5941 ttggggaaat ttcatgaacc acgaagcata tggaccagac acgacgcgaa gcttcttaga
     6001 aagtctgcat ttaccgcaat tcatcattga taatatgtat atcgaaggga tctatcgtca
     6061 accaacattt ctttatgaat cactttggag tttgattgga tttattttgc ttttgttttt
     6121 acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta
     6181 tagtttcggg cgtttcttta ttgaaggatt acgtacagac agtctttact ttttaggtgt
     6241 gatccgtatt tctcaattat tatctgctgt gttatttgtc ggtacattga ttctatttat
     6301 ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa aaaatcaaca
     6361 aattatctga tttttagagc gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt
     6421 atagagtagc ggttttagga ccaggctctt ggggtactgc tttggcacaa gtcctcgccg
     6481 aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa gaaatcaaca
     6541 cccaacacac gaatcaacgt tatttaccag atcattatct acccgattcc atccaagcat
     6601 tcaccaatat ggaagatgca gtaaaagacg cagatgcggt gctattcgtc attccaacga
     6661 aagcaatacg ttctgttgca aaagaactag tgcctaaatt gaatacaaag ccagtgatca
     6721 tccatgcaag taaaggatta gaacagggaa cacataaaag gatctctgac atcttgttag
     6781 aggaaatccc agcagaaaaa agaaaagctg ttgttgttct ttctggacca agccatgcag
     6841 aagaagtagc aaaacatgac atcactacga tcacagctgc aagtacagat gcaaaagcag
     6901 caaaatatgt acaagagctg ttcatgaata attattttcg gatctatacc aatcctgatg
     6961 tgatcggtgt agagatgggg gcagctttga aaaatattat tgctattggt gctggcgcta
     7021 ttcacggctt agggtttggc gataatgcaa aggcagcaat catgacgcgc ggattagctg
     7081 agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatc ggattaagcg
     7141 gaataggcga tctagtcgtt acttgcacaa gtgttcattc taggaactgg cgcgcaggca
     7201 acttgttagg acaagggcag cccctagaag aggtgctagc caatatggga atggtcgttg
     7261 aaggtgtgaa tactacccaa gcagcgatgg aactagccca aatgatggaa atcgaaatgc
     7321 ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa
     7381 aagaaatcat gttacgtgat ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa
     7441 ggagcgaatg tatatgaaag ttaaaaaagc agttattcca gcagcaggat taggaacaag
     7501 atttttacca gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac
     7561 aatccagttc atcgttgaag aagcgttgaa atcaggtatc gaagacatct tgatcgttac
     7621 aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatattgaat tagaaacaaa
     7681 cctaagtgaa aaaggaaaga ccgaattatt gaaattagta gaagaaacga cagatgtcaa
     7741 cttgcatttc atccgtcaat ctcatccaaa gggtttagga catgccgttc ttcaagcaag
     7801 agcctttatt ggaaatgaac catttgtcgt tatgctaggc gatgatataa tggaagacga
     7861 agtaccattg acacagcaat tgatcgatga ctatgagcgt acacacgctt ctacgattgc
     7921 cgttatgcaa gttcctcacg atgaaacttc aaaatacgga atcatcaacc caggggaagt
     7981 actggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc
     8041 accaagtgat ttagctatta ttgggcgtta cttattgact ccagaaatct ttgatgtatt
     8101 atcagaacaa caaccaggtg ctggaaatga gatccaacta actgacgcaa tcgatacgtt
     8161 gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttatg acgttggtga
     8221 caaatttggt tttatgaaaa caagtattga atatggtctg acacatccag aagtaggggc
     8281 acctttgcgt gaatacatca tgtctttagg taaaacatta gaagcagaag gaagcaagat
     8341 caatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat aatctttttt
     8401 tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt
     8461 aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt
     8521 caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttca
     8581 tcgttcgcgt attgttgcaa gtttccaaaa ctttagaaaa agtagaccag actgtcgcag
     8641 aagcaaatac gacaattgag atcgtcacac aagacgtaga tcttttatct agacaagtag
     8701 aaggcttgct agtcaaaagt aatgagctgc tcttggatat caatggaaaa gtcgcaacga
     8761 tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcggaa cttaatcatt
     8821 ctagtaaaaa catcgtgacg aaagtcggcg gaatcggaaa aacgacagct aaagcgactg
     8881 ttgccggaaa agtcggtgga acagcgatga aattttttaa aaacaaaaaa catactgaaa
     8941 cgacaggagg aaaataagaa tggctaaaaa aggtggattt ttattaggtg cagtagttgg
     9001 aggtacagcc gcagcaatcg ccgcattatt gttagcaccc aaatctggta aagaacttcg
     9061 ggatgaattg gctgctcaag cagatgattt gatggacatg gcttcagatt acacagatat
     9121 ggcaaaagaa aaatcatctg agttaacagg tattgcaaaa gaaaaagcgc aaggtctttc
     9181 tcaacaagca ggggatctag caggcaatgt taagcagaag gtaagtgaat cagcagagga
     9241 agcaggaagt gaatcagatg atatcttgtt aaacctcaaa gaacaatcag ctgatttatc
     9301 taatcgtttc aaaaaaacag cagaagatct gaaaaagcca gcagacgaat taggtagtat
     9361 tgctaaagat acttcagaag atatcatgat cgatgtaaaa gatgctgcag ataaagcagg
     9421 agagacggtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgaca caaaagaaat
     9481 gttgaaaatg gcaaaagaag atgcaaaaga agctaaaaaa gaaatgaaag atacaatgac
     9541 agattcgact accaatcact aagattactc atttctagaa taaaaaaggt gagagcggac
     9601 cagctctcac cttttttatt ttcattactc taaaacaagt aattcttttg gtgtagtcgt
     9661 gaatggcaca caaccatcac ttgtcacata cacacaatct tcgatacgta cccctacttt
     9721 ttcaggaata tagattcctg gttcaagaga gaagcacatc ccttcttcaa tcactaagtc
     9781 gtttccttct acaagagaag ggaattcgtg gacggttgtt ccgattccgt gacctaaacg
     9841 gtgattgaaa tattcgccgt agccatagct gttgatcacg tttcgtgcga tttgatcaag
     9901 ttcacctgct gtcacacctg ggcgaactgc ctcttgtgcg gcaagctgtg cttcaagagt
     9961 gatgttatag attttttctt ggaactcagt tggtttttga taagcaactg tacgagtagc
    10021 atcactgcaa tagccattcc aaataacgcc aagatcgaat aacactagtt catttggtga
    10081 aaccttcgtg cttcctggtg taccatgagg gcttgcagca tttgctcctg caaggactaa
    10141 tgtgtcgaaa gacatttgtg aaacaccttg tttttttaat tggtattcga tttctgcgat
    10201 aattgcttgt tcagttacgc cttcttttac tgttttgaag ccaatttcaa aggcaacatc
    10261 ggcccattta ccagcttcga tcagtttgtc tttttcttct accgttttga tcagttgcag
    10321 acgctggata atagaagtta gatcggtgta agaatctgta cctgtcaaga tatcagagat
    10381 agcatcgaag cgatcgacag tcagagcatt cttttcaata cctacattac caggttgccc
    10441 atagcgtgcg cgaagctctt ttccgatgat ctcccaagga ttttcgctgt ctaagtagcc
    10501 acgtacatcg tacgcccagc tgctgttttg agcatcttca acttccagag caggagtgaa
    10561 aaggaaagga tcttgttcca gagggataaa aagagctagt acacgctcgt gaggatcact
    10621 ttcatatccg gaaaagtaag cgatgtggcc agggtcgctg atataagcca attctacttg
    10681 gtgttgtgcc atccattgac gtagttcatt gatttttacg ttattcattt ggcaacctac
    10741 tttctaaatt aattttgtat tcaataattg taccactgat tgattaaaag accaacgttc
    10801 ttaaaactga aaaaagtaaa aattttcatg aaaacagaga taaaacggtt gcaattgttg
    10861 agattatttg atatcctatt ttagaaatgt aagacgtatt ttcagaaaac atataaaata
    10921 tgaataggag aacacaaatg gaaaagcaaa caatcactat ctatgatgta gcgagagagg
    10981 caaatgtttc gatggctact gtttcacgtg tcgtaaatgg caatccaaac gtcaaaccag
    11041 caacaagaaa aaaagtcttg gaagttatcg atcgcttgga ttatcgtcca aatgcagtcg
    11101 cacgtggctt ggcaagtaag aaaacaacaa cagttggggt aatcattcct gatgtcagca
    11161 atatgttctt tgcctcttta gcacgtggta tcgacgatgt ggcaacaatg tacaaataca
    11221 atatcatctt agcaaactca gatggcaacg atcaaaaaga agtcaatgta ttgaataatt
    11281 tattggctaa gcaagtggac ggcgtgatct ttatgggtca ccatatcaca gacgaaatcc
    11341 gtggagaatt ttcacgttca aaaacgcctg ttgtactagc tggttccatt gatccagatg
    11401 aacaagtagg tagcgtaaac attgattatg tgtcagcaac aaaagatgca gtaaataaat
    11461 tagcaaaaag cggcaataaa aaaatcgctt ttgtcagcgg tgcgctgatt gatcctatca
    11521 atggtcaaaa ccgtctaaaa ggctataaag aagcattaag cgaaaacaat ctaccatata
    11581 gcgaaggatt gatttttgag gcacaataca actttaaaga tggtctgtct ctagttgacc
    11641 ggatccataa tagcggtgca acagctgtct atgtttcaga cgatgaatta gccattggta
    11701 tcttggatgg tttattagac cgcggcgtaa aagtaccaga agaattcgaa atcatcacaa
    11761 gcaacaattc tttactgaca gaagtagctc gtcctcgttt gacaagtatc acacaaccat
    11821 tatacgattt aggagcggta tccatgcgcc tattgacgaa aatgatgaat aaagaagaag
    11881 tcgaagaaaa aacgattatc ttgccttatg gaatcgaaga aaaaggatca actaaataat
    11941 aaaagatcaa tcatttaaag gttgttccaa aagtggtcaa cttctggaac aacgtttata
    12001 cgagaaaggg ggttatgaca aaattttgtc ataacccctt tttttttgtg actttaaaaa
    12061 aagagaaaat ggcggaaaca aaccttacgt ccatcgtata cgacacaatc tgtgcgtaga
    12121 tgctacattt tgttcactac tatcaaaatt actttaaaat gaaaacgtga taaaaaagaa
    12181 agtagaaaag tagataagta actctaaaaa atccaaaaaa atccaggctc gattgcgtac
    12241 ttatcttaaa ggagaaacaa tccatcatat ataacccgaa aggagacttc tatttgccta
    12301 agaaaagacg aaagaccata gtcatcattt tgaccattct tactatagga atcggaggga
    12361 aattttacat ggacaaaaga gaaatgcaaa gacagaaaga tttactcgca gtagaaaaac
    12421 agtccgtcaa agttctgaaa aatacctttg cggatatcaa ggaagtgaag attgaaaaat
    12481 ctgctcgaaa tgaaatgacg ggttcatacc ggatagttat tctaatgact aataaacaag
    12541 atcaatcgat ttatttttct tatagttttt ggaaagaaag aaatgagata ggaagttacg
    12601 gaatagttga tgaaaaaaag caaaaagaag gaaatacgct caataaagtt aaagtaactt
    12661 actctaatgg aaatgaggaa agtatttaat gattactgac aaacaatata gtgaattaag
    12721 tgatgctgtt tactggctag atcctaaaca tagagactat gtacctgaaa tgcaagaaaa
    12781 tttaagtttt aaaattaacg gcactatgta taaaatccta aaaatcaaaa actccttcga
    12841 cggcatgcag gcgatggctg tagcgccaat cgtccatagc aaattggaga aaaatttcaa
    12901 aaataaaaaa attcctgcaa actttcgcgt acttaaataa tgaaggtgtc aatacttaca
    12961 taaaaagaaa ggaggcacgt tcttgtcaag gaaaataaga aaactcagct tagccatttt
    13021 attaatcgtc atattaggga tcggagggaa agtctatatg gacaaaagag aagcacaaag
    13081 gcagaaagat ttactcgcag tagaaaaaca gtcagtcaaa gttctgaaaa atacctttgc
    13141 ggatatcagg gaagtgaagg tagaagaatt taaaaaaaac cctgttaccg gaagttatgg
    13201 tgcacttgtg acaatgacaa ataatgaagg gaaatctgtt tattttgatt atagtttttg
    13261 gaaggagaga aatgaacttg gtggttttgg cattgagaac agagcggtac aaaagacagg
    13321 agtcactata aaaagagtaa aagtaatttt ttctaacgga caggaggagt tagtatgaca
    13381 ataaacgata agcattacaa tgatattagt gagaaggttt attggctaga tcccaaatat
    13441 ccccgctaca atgagggata caaaaagaac tctgttaaag aatttgctgg tatggaattt
    13501 caaatcctac aaatcaaaga ctcattggac ggcatgcagg cgatgtctgt agcgccaatc
    13561 aagaacggta aagtggatct ttctcaagta gtgattgctt ttgcaggaac gaacttaagt
    13621 gattggaaag atttggaaac ggatgcaaga agtatcatga atctctacgg gaatagacca
    13681 ggagcaagtt tttctgaaaa agcaacaaca aacaagattt tatccgttag tggattgaaa
    13741 acagcggggc aaatcaattc agcgattgca tttgctaatg aagtaaaaaa ggaatatgga
    13801 gaagctgcta tttctactac cggccattca ttaggtggat tcttagcatt atatgtagca
    13861 gcagagaaac aatggaaaaa tgtcggcttc aacgggccgg acccgtatga tctcttatca
    13921 ccagaagcaa aaaaatgggt ggaagaaaat cccggtatgc tcaccaatta ccgaaatcac
    13981 gccgatttta tcggtaattt tggtggtaat ggcacaggtg ctgaagttcg gatcagtatg
    14041 gaaacagcat tcttgaatct gcttgcttcc cacagtttaa cctcttggaa gtttgatgaa
    14101 aatggaaagc tgattattcc tgaaaatgaa aacaatgtta aagctcgaaa acaacagaaa
    14161 gaaaaccaag cgacccagtt gttttcctat cagttgcaag aactgaacaa catacgtaaa
    14221 aaactacgat taagtggcgg ccggctgaca gttaatgaac aaatttactt agatgacaca
    14281 caagctaggt tagtagtgga aaagttatcg gcaaaaatga aaagcgctat ggaagaagtt
    14341 atccgcatca atcaaagagg aatttttgag ctagagaaaa aatggcaaga aggcttgcgg
    14401 caagcacgtg ttgtagcccc ttcgttgact gaacaggaaa tcatagaagc attagctagt
    14461 gtgggcgcca caaaagaaaa cttagtcgat tctgcgaaag aagtctatct gctacgaatc
    14521 aataaggcaa gacgtatggg ggaggccttt gatacattgg cgaaggagat ccaagacaaa
    14581 atcaatgaaa tcgtggcacg agatcgagaa ttagcacagc aattcaatta aaggagaagt
    14641 agatgaaact agaagaattg gaaagagatt atcgaagtga acgaagacaa ttagaagctc
    14701 aagaagatga attgatccac tttaagaatc aaggattacg attgatggag caagaggagc
    14761 agcgactgca tcatgtatta aagaacttag ctttagataa tgagcctctg tttcaagcaa
    14821 gacgaagtta ttcgaattat caggagcatt atcaacaagc cctacaagta gaacgacgaa
    14881 aactctatca gcagcaagat gaactagaac gttcctatcg tcaaaaaatc agacaaataa
    14941 ctatggagaa atcatcttat gaatgaattt ttaatgcaat tactaaaaga attagagaaa
    15001 aatctacatc gatcaaatgt acgttcagtt ggtatgggga tatctcagca aatttgggca
    15061 gaaaaacgat tgacagatca aaactaccaa atgactgcag aagaagctgc tcaagaattt
    15121 tctaaccgac atgtacactt gaaaggatca gtaaaagggc aatttgcaga aaaactaact
    15181 gaattattcc aaagacaaga aaagaaatta aaacagtttc gttagtaaag ttatatagaa
    15241 aaattttgtg aaaat
//
LOCUS       DAZKLA010000060        15157 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000060 DAZKLA010000000
VERSION     DAZKLA010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.061"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..720
                     /locus_tag="ACG85N_001658"
     CDS             <1..720
                     /locus_tag="ACG85N_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0224426.1"
                     /translation="SDQAFLITKNTILYNVAFILLNLLISVVFAIIMSELRNKRLVKV
                     YQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVF
                     IGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTIL
                     AVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVG
                     FVLLMITNTIARRFDSDSALF"
     gene            732..1652
                     /locus_tag="ACG85N_001659"
     CDS             732..1652
                     /locus_tag="ACG85N_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0224427.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1693..3150
                     /locus_tag="ACG85N_001660"
     CDS             1693..3150
                     /locus_tag="ACG85N_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0224428.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3495..4109
                     /locus_tag="ACG85N_001661"
     CDS             3495..4109
                     /locus_tag="ACG85N_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0224429.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4102..5826
                     /locus_tag="ACG85N_001662"
     CDS             4102..5826
                     /locus_tag="ACG85N_001662"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0224430.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5823..7274
                     /locus_tag="ACG85N_001663"
     CDS             5823..7274
                     /locus_tag="ACG85N_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0224431.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7747..9900)
                     /locus_tag="ACG85N_001664"
     CDS             complement(7747..9900)
                     /locus_tag="ACG85N_001664"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFQ0224432.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9902..10948)
                     /locus_tag="ACG85N_001665"
     CDS             complement(9902..10948)
                     /locus_tag="ACG85N_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0224433.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            11098..12387
                     /locus_tag="ACG85N_001666"
     CDS             11098..12387
                     /locus_tag="ACG85N_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFQ0224434.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12400..15102
                     /locus_tag="ACG85N_001667"
     CDS             12400..15102
                     /locus_tag="ACG85N_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0224435.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
BASE COUNT         5108 a         2647 c         3052 g         4350 t
ORIGIN      
        1 tcggatcaag catttctcat cactaaaaat acaattttat ataatgtagc atttatcctt
       61 ttgaatctgc tgatctcagt cgtatttgca atcattatga gcgagttgag aaataaacga
      121 ctcgttaaag tttatcaaac gatgtcctta ctaccttatt ttctatcttg ggtcattatc
      181 agttactttg tttacgcttt tttaagtcct gacaaaggaa tattcaatca gtggatcact
      241 ggacatggag gagaagctgt caattggtat aatgaaccga aatattggcc gtttatcttg
      301 gtgttcattg gtacatggaa agggattggt tataacagca ttatttactt tgcatctgtt
      361 atgggaatcg atccgactta ttatgaagct gctatggttg atggagctag caaatggcag
      421 cagatcaaac atgtgacgat accacaactt attccactaa tgacgatttt aacgatttta
      481 gccgtaggaa acattttccg agctgatttt ggcctgtttt ataacattcc taggaattcc
      541 ggtgcattat atgatgtgac tcaagttttg gatacgtata tctacaatgg cctgacatcc
      601 acaggagatt ttggtatgac ggctgctgca ggactttacc aatcagttgt cggctttgtc
      661 ttgttgatga tcacaaatac gatcgcccga cgttttgata gcgattctgc attattctaa
      721 ggaggaaaaa aatgaaaaaa aagaaagtgg agagagtcca agtccgctct ttcaataaga
      781 cggctaattt tttctctaat ataatgattg cactctttgc tatttcctgt attttgccat
      841 ttatatttgt tatcgccatc tcttttaccg atgagtccat attgtcgact cacgggtaca
      901 gtttttggcc agaaaagttc agtatatttg gttatacgta cttgttcgac cagatgcagg
      961 ataaaatttt ccaagcgctt ttcgtcactg tacttgttac tgttttaggt acactgatca
     1021 atagcacggc tacttcttta tatgcttatg cgatttcacg gtcaaacttt ccattccgtc
     1081 gtttttttac cgttttttgt ttgattacga tgttgttctc accagggatg gtggccaatt
     1141 atcttgttat gacaaatctt cttcagttaa aagatacgat ttgggcattg atcttgccaa
     1201 tggctgtcag tcccttcaat attattgtga tgcgcacatt ctttaaacgt tcggtctctg
     1261 attcgattat tgaatcggca cggatcgatg gagctagcga actgcgaata tttgttcaga
     1321 tcgttttacc gttagctgtt ccaggtattg cgacaatcag tttatttgcc gcattgggtt
     1381 actggaatga ctggttcaat gctttactat acatccaaga tgataagcta gttccactac
     1441 agtatctttt gatgaaaatc caaagtaata ttcaatatct gacacaaaat tcaggagcag
     1501 gaagccaatt gacaggcgga ttagcctcag ttccaggaga atctgccaga atggcgatcg
     1561 tagtcatctc gacgttgcct attgcgatca gctatccatt tttccagaag cattttgtca
     1621 aaggtctaac aatcggcgga gtaaaagaat aaagaaacaa aaatataaat cataaaatta
     1681 ggaggaagaa aaatgaagtt atggaagaaa ctagccttta caggtgtttc agcgttgttg
     1741 ttgggaacat tagcagcatg cggaggttct ggttctaaag accaggcaga agctagcaat
     1801 tcggacactt tgcagatgta tcaaatcgga gataaaccag ataatttcga tcaattgatg
     1861 gaagtagcaa acaaacgaat cgaaaaagag atcggtgtaa aagtcaatat caattacatt
     1921 ggctggggcg attacgaaaa gaaaatgaac gtcatcattt cttccgggga aaattatgat
     1981 atcgcctttg cgaacaacta tgtttccaat gcccaaaaag gagcctttgc tgatttgact
     2041 gaattagcgc ctaagtacgc tgaaaaagct tataatgatt tagatgaagc atatattaaa
     2101 ggaaatctgg tcaatggaaa attatatgct ttcccagtca atggaaatgt ctttgctcaa
     2161 caagtactta cttttaacaa agctttacta gataaatacg atctttcaat tgatggaatc
     2221 caatcttacg ctgatgcaga aaaagtcttg aaggaattcc atgaaaaaga accaaacacg
     2281 gcagcttttg ccattggaca aggcttcaaa gtccaaggga actttgatta tcctttaggg
     2341 aatacgttac catttgctat tgatctggat ggagatgatt cgaaaatcat caaccaatac
     2401 gataatgaaa cgtatatcgg actgttacgt acaatgcata gctggtacaa acaaggactc
     2461 attccaagtg acgctgcaac gagcaataag gattatccac tagaaggaaa tacttggcta
     2521 atgcgtggag aaacacaagg accgtatgat tatggcgata caattttgac aaatgcagca
     2581 aaacaagaac ttgtctcaaa agcaattact gttccactaa aatctacagc tcaagcacaa
     2641 atggcaaatt ttgtcgtatc aaatacttca aaaaataaag aaaaatcagt agaacttcta
     2701 gggctgctta acagtgatcc agaattattg aatgggcttg tttgggggat cgagggagaa
     2761 gcatgggaaa aagtcgatgg ttcagatgat aaaatcaaat tgctagatgg ttatcagcca
     2821 aatacacata tgtctgcttg gaatacagga aacaacaaga ttctatatac acaagagtct
     2881 attacggatg acatgattgc caaacgtgat caatcgattg ctgatgcaga aacttcaccg
     2941 attttaggat tcagcttcaa cactgacagt gtcaaaacag aattaagtaa tatttctaat
     3001 gtcatgaatc aatatttaga tggattgaat acagggactg ttgatccaga tgaaacatta
     3061 ccgaaattga aagacgcttt ggacaaggct ggctatgata aggtattgaa agaaatgcaa
     3121 aaacaatatg atgagttccg acaagaataa taatgaataa agacaaaggg gttgtgacaa
     3181 aagacgtgtc acaacctctt gttccgaata aacggtgttc aaaagccagt ttctcggtat
     3241 taagctaaat atatgtaaaa atgtggaaag ccatattcac aaaatttttc ttactactcg
     3301 aaactaagcg gctatttcac aaactcttgg taaacggtgt tcaatcagct aaccctcggt
     3361 attcgctcac aaaacgacaa aatatgagga actatttttc cgtttgtttc tctaataatc
     3421 aggtcaaggt gctgattgcc caaccttttt ttgtataatg agaaagtata aacttaatgt
     3481 ttggaggaga agatatgtta gggaaggttt tggaagagtt atttatccgg atctgggtca
     3541 ttataaaact aaccttgtat ttctggatat atacatttgc cggcgggatc atctttggat
     3601 taggtgcggc atggaaaaca gtcaatgaac tattttattt gtacggattc gagtataaag
     3661 aaatcacaat aaaaagaggc tggaatatat ataaaagaaa ttttttaaga ggcaatctgc
     3721 tatttagttt atttttatct ggtactgctt tattgagtta caatttatat ttatctgttc
     3781 aaatcaaagg gctcattttt ttagctattg attttatttt actttttgcc ttgctctgcc
     3841 tatttgctac ttatcaatac agtatgattt tagatagtga atatacgatc tctgttccta
     3901 atctgctaaa gctaagtttt atttcggttt tttccagctt ttcgtctttt ctaaaaataa
     3961 ttattggtag cggtataatt ttaggcgtga cttggcaatt caaaggattg atcctgtttg
     4021 gtgtcattgg tcttttgacg gtttggaatg gtacgatgac aacacattgg cgtgaagaat
     4081 tagataaaca gttggaaagt tatgaataag tttcggattc tattttcaaa aggaacctta
     4141 ctaaatcgtc tgttgcggaa atacgcggtt atcatgatcg gtgtaacgat gactgctacc
     4201 gttattttta gtatccatac ttgggaacaa aatcaaaaac aggcagaaaa catgacaagt
     4261 gatgccgttc aaagcacaag cagaatgctg aatgataaaa caaccttgag taggatcatc
     4321 aaaaaccagc ttgtagggaa ttccgaaaag atcgaaaatg tgacgaccta cttaacaaaa
     4381 cccatagatc agtatctgat gtatgtatac gaacagcaaa acagtaccga tgaattagtt
     4441 tctttcccaa atcaaatcaa agatctctac gcaaattatg aagaactaag tgcgatatat
     4501 atcgtgttga atcaactacc ggagtattat gaatcttcta gagaaaacaa aggtggggaa
     4561 ataaaaaagg ggacaccaaa attactagac gccttttata tcaagttgcc tatcacacaa
     4621 ggaggagctg agtcaggaag tatttttatt ggttttcaaa agaaagaact agatatgatt
     4681 ttagaaaatc taacgtcatt caatggactc tctttataca tgatttctgg aacgacaaat
     4741 cgactataca cattccatga taataaaata gctaaagaaa cgtttgaaag acaagaaatg
     4801 atcatcacag agagtctgaa gaaaaattca caattgccga ttgacgatct ggaaaaaaac
     4861 aattttgttc aacaccaaga actgtctgat gattatcgta tattagctgt actagataaa
     4921 aaaagtgtaa aaatcgaaac gggaaaaaat cttgccccgc tgatcattgg tatcattatt
     4981 ttagacaata tactgcttat ctttctttat aaaacattca aacgatattc acagcaagta
     5041 tctatcgtca tgcaggccat ggatcaaaca gcagcaggga atttagagac gcggatcgac
     5101 ataacaaaca cggaatatga actgaaggaa ctttcaatcg gtatcaacga aatgttggat
     5161 agcatcaatc agtttgtaga agacatctac aaattagaaa tcaaacagca agacgcacat
     5221 atgcgggcgc ttcaagcaca gatcaatcca cactttttat ataatacatt agaatatatc
     5281 cgaatgtatg caatcagcga aggaagtgaa gaattagcag atgttgtgta tgcgttttcc
     5341 gctttgcttc gaaacaatac gaatcaagag aaaacgatca ctttaaaaga agaactggac
     5401 ttttgcgaaa aatatgtcta tttgtatcaa atgcgttatc ctaatcgagt agcctatcat
     5461 tttatgatcg atcctgattt ggaaaagatc gaagtaccta aatttgtgat ccagccatta
     5521 gttgaaaatt attttaaaca cggtattgat tttactcgat tcgataatgc gctaagtgtc
     5581 aaagtcttgc aggaaggcaa acgggtaagg atcatcatca aagataatgg aaaagggatg
     5641 actgaaaaac ggctaaaaca gattgaagaa aaactgtctc atcctaaagt ggaacttcac
     5701 ggttctatcg gtctgcaaaa cgtgaatgaa cgtttacgtg ccagcttcgg cagcagttat
     5761 tatatgtcgc ttgagaataa tgagacaggt ggactgaccg tttctattac attcaaggag
     5821 ggatgagcgt gtatcaagtg ttattggtag atgatgaata catgattttg aatgggttaa
     5881 aaaagataat tgattggcaa agtttaggct ttcaaatagt agcaacagca gagaatgcca
     5941 aagaaggact agctgtactt gaacagcgac agattgattt agtagtaacg gatgtgacaa
     6001 tgccagaaat aaatggactg gaatttattg aagcggctca aaaagaacgt cataattttg
     6061 aatttatgat tttatcagga taccaagaat tcgattattt gaaaggaggt atgcaattag
     6121 gcgccgtgaa ttatttgatg aaacccgtga ataagttcga attggttgaa agcctcaaga
     6181 aaatcaaaac acggttagat cagcaaaatg aacaaaaaaa tcaacaagaa atctatcaag
     6241 aactgttatt cagtcaatgg ttgaatgaag aattggatga aataagtgaa gaagaaataa
     6301 taggacaaat aagtgagaag cagcatagga tagtattgat tcaactggca agaactcatg
     6361 atactcttat caatagatgg ttgcaagaac ggcaacaaat cttttactat caacgaaatt
     6421 acggagaccg tgtattgatc acgttgttgt tggaggcaga tgaagttgat ggcttctgtt
     6481 gttttgttca agaaaacttt cctgatcagg aatggctgat cagtatcggg gaagagacag
     6541 attctttgga caaaatccct gaaacattcc aacaagcgaa agacaatcta cagttgcatc
     6601 aattttatgg agataaagac gagcatgttt tttacgcagt acaagcagaa acaaaagatc
     6661 aaaccatcga tttttcaagt ttcagacgcg tgctgcaaaa taaacaatta gaagtcgctc
     6721 aaaaaatgat cactgatttt tttgaacagt ttcagttagc agccatgatg ccggaagata
     6781 tacgatattc cagtttttta ctgtttatgg agattcaacg ggaattgatt gatctggaag
     6841 atgaggaata tttacaagga atcgaaaaaa tccatcaggc aaaaacggtg caagaacttc
     6901 agcagctact tctgtcattc atcaaaaagc atcagagaca gaaaaagtac agtgacaatg
     6961 tggaaaaagt aatcgtaatc ctgcaccagc attatcaaga accacttaca ctcaaagaag
     7021 tgtcagaaaa cctgcattta aatgtcatgt atctaggaca gctgttcaaa aaagaaacga
     7081 aaaaaagttt ttcagcttat ttgaaccatt tacgtatgga aaaagcgaaa caactgcttt
     7141 tgcacagtaa tcaaaacatc aatgagatag caagtgagat tggctacaac aacaccacgt
     7201 atttctctaa attattcaag aaaatcgtcg gcagatcccc taaagagtac cgagaaaaca
     7261 gtggagaatc ataaaagaaa aagccaatgt aatgaaataa cttataaaaa attgtctaga
     7321 aatctaattt tgggctgttc ttattacttg aggcaaggca gctttttcat gacctctcga
     7381 taaacggagt tcaaaagcca gtttctcggt attaagccaa atttatgcaa aaatgtggaa
     7441 agccattttc acaaaatttt tcttaatact cgaaactaaa cagctttttc acgacctctc
     7501 cactaaacgg tgttcaatca gctgaccctc ggtattagct caaaaaatgg caaaatatga
     7561 ggaactattt tgccatttgt ctctctaata ctcaggtcag aacgctgatt tcacaacctc
     7621 tcaataaacg gtgttcaaaa gcttgcatct gcggtaataa gaccaaccaa acgaaaaata
     7681 tgaggatcta tttttgcttg gctgttctta ttacttgatg caggacagct ttttcacaac
     7741 ctcttgttaa ttatagtttg ataaggaaaa aggcaaatct tttgcttgat atttcttagg
     7801 gtgagggatg atccctaatt ggtaagagat cgtgccgcct ttcagcaaat cttgatgggt
     7861 aaaatataat ttcgtatatt ctttgtccat ccgtttgatt ttatggataa attgttgctg
     7921 cggccgatta ggcgttgctt caatcgtcaa ttcctttcca tttgacaaaa gcaaaacagc
     7981 cttgtcaatt aatggcatac cgataacata ttcaccggtt ccaggcgtga caggataaaa
     8041 tcccagacta ttgaagatat accaagcggc catacttcca ttgtcctcat ctccagggta
     8101 acccattgga gaatcgtcga aggtctgagt catcaactgt ttaagcagtg gctgtgccat
     8161 ttcaggttgt cctatataac tgaacaagta aggatagtga aaacttggct ggtttgaaat
     8221 agctaattgt ccaaattcta tcgcggccat ctcactcatt tcgtggatct caaaaccata
     8281 tcctcctggg tcaaacgttg ggggctgatt gcacagctca atgagttttt ctttatatct
     8341 ctcactgcca ccatattttc cgatcagtcc tgaaaaatcg tgataaacag caaagctgct
     8401 ttgccaagca cttccttctg cataatcttg tccccaacga ttagctgaaa atggttctct
     8461 gaacttgcca tctttatctt ttgaacgcat aaatcctgac tcatagtcaa agatattttg
     8521 atagttcaat gattgctgct gatatttctt cgtcatttcc tcttcttcta gtaccttggc
     8581 aacttggcta atacaaaaat cgctataagc atagtctaat gtgtgattga ctgattcgtg
     8641 gtagtgatta ggaacatagc catatgtcaa gtaatcttct gttccttggc gtccataatt
     8701 tggatgtttg ctttgaaccg tagcaccttt ttccattgcc gttagaaatt caggcatcaa
     8761 gtctcgtcgg atatctttta cagcagcatc tgcaatgacc gcatcgatca atgtcccagg
     8821 cataagtcca cgttcatcag gagacagcca ttttggcaaa tagccacttt ctctatagct
     8881 gttcaaaaaa ccttccagca tttcttcgta tttttctaca gcaatcaaag aataaagagg
     8941 ataaaccgtt ttatacgtat cccaaaaccc attatttgta tagagaacac caggttttat
     9001 agatcgacta gtcgtatcat agtggatttt ttgcccacta ggatcaattt cataaaatgt
     9061 ttgtggaaat aaaaacaccc gatataaatt atgataaaac gtccgcaatt gttgttgatc
     9121 atggtgtgtg agctgtatac gatcaaaata ggactgccat tgttcttgac tgtgagcaag
     9181 gtaagtctct ttgctttggt tttgttctag ttccaggtta taagctgctt gttccgagct
     9241 gataaaagat gtcccaaatc ggatagtttg tttttttgtc tgttcaaaat acaacgggat
     9301 cgagtcgcta tttttgttgg aaacagctaa ctcatcaatt gttaaaggat attcaaatct
     9361 caatataaaa taaaaagaaa aatcaggatc ttctgaacca gcaaaatgtg tgatttttcc
     9421 ttccactgtg taaggatcta taacaaagag ctgatggcgt cccggaaagg ataaaagtaa
     9481 accgctgtca tttttgctgt aatcaataga taagattccg ccatacatgc taggtatcaa
     9541 tgtagaacag atgccataac gtaattgaca gatcgacaga tgagtcgggc aaaatatact
     9601 ttcttctggg cggtaagaac tttgagcatg gaaaagggtg ttctcctgta gttttccatt
     9661 gatcggcgta aacagcatat gactgaaatc acccatccaa ggacttggtt gatgggtaag
     9721 acggtagcct tgaaaaacgc ggtcatcagg atgaaaccac cagctgccgt tttgatccgt
     9781 agtttgcggt gcaaaataat tcatcccaaa aggaacgcca gtataaggct ggcagttccc
     9841 attagagaaa tttggctgat tcgctgttgc atgtctagta tctatttgac cgatattcat
     9901 tttatttctc ctttgttgat tgtcttttga tgaatgaaac tggaaattta ctatcggatg
     9961 gtcgatcttt tgtctctaac caagtgacca aagcatctcc cgcaagctta cctaactgtt
    10021 caaaatcttg tgcgatggtt gtcagtggcg ggtcaacaag agctgctgct tggatatcat
    10081 caaatccgat aattgaaaaa tcttcaggga tatgataacc atgctgtctc aagagattca
    10141 tggtctggat agcgacaaca tcgttttcac aaacaaaagc agttacttca tacttagtga
    10201 tataagaaag caattcatct aatgtcgcgc ttttgtcatc cagtgaagtg tggaaagtca
    10261 aaccagcttt gtccaatgct tcaaggtaac caaggtaacg ctgtcgaacg gattgtggat
    10321 gagtctgcgt accaaacaaa taagcgattt ttgaatgtcc ctgttcgatc aaataagagg
    10381 cagcaagcgc accgccttgg aaattatcgg aaagaacggt aggaaaaggt aactcataga
    10441 tttttttatc taatatgacg acagggaatt ctttcaatga aagctcaaaa agtaagtcaa
    10501 gatgttgttc tgtatcaaga gcataataaa tcaatccgtc aaattcattg attagctctt
    10561 ttgctgtttt attttgtaga aagtcaagcg tcgtcatcat gacatcaatc tgtttatctt
    10621 gcatcacagg gttcaatccc ttgctaaaat caccaacaga caaatcagcg acatatggaa
    10681 gaagaaacag aatccgtcgt gtttttgtag gagaagcaag aatctgttcc ggagaagcgg
    10741 atttaacgaa actgcctttc ccgcgaacgc gataaataag tcctaattgc tctaattcag
    10801 tcaatgcacg tttggaagtg attctgctta ctttataggt acgtgaaagt tctttttcag
    10861 ttggtagttg gctgtctaca ggtaattcac cagttaaaat agccgtttgc aaatcattaa
    10921 atattgtctg gtacaatggt tgattcatat aaacacctct taatgatata tcatattcaa
    10981 ataataccat aatggatgaa aaagtaaata aaaagatatt ttttgaaagc gtagacataa
    11041 ttggctgtat gaagtataat ctttaaagat atatcaaata gaaagaaggt tttattagtg
    11101 gcttataaag atataccaaa atcggtgcag aaatttatgg aagatatcac acaaaaatgt
    11161 ggggatatcc atgcggactg ggcaaagaat tttacagcag catttgccaa tactctgttg
    11221 acaacagtca aaaagcaaaa tgacggaacc acctttttat tgacgggaga cattccagcg
    11281 atgtggttaa gagactctac tgcacaagtt cgtccttatt tagtgattgc aaaagaagat
    11341 gaggaattag ctgacatgat cagcgggtta gtcaaaaagc aattttttta tatcaatatc
    11401 gatccatatg cgaatgcctt caacgaagaa gctaacggtg ctggccacca aacagatcat
    11461 acaaaaatga acgattggat ctgggaaaga aaatatgaaa ttgattcttt atgctaccca
    11521 atacagttgg cctatcttct ttacaaaaat acaggaaaaa ctgatcaatt caatcaatct
    11581 tttattgaag gtatcaaaaa aatcttggac gtgttcaaaa cagagcaaga tcatgaacac
    11641 tctccttata catttgtacg agacacgaca agaaaagaag atacgctgat caatgacggg
    11701 cgaggtgcaa aaacagcttt tacaggaatg acatggtctg gtttccgtcc tagcgatgat
    11761 acttgccgtt atggatatct ggtaccatcc aatatgtttg cggtggtcgt gttaggttat
    11821 gtggaagaaa tttttcaaac gatactcgat gataaagaaa ttgtacagca aacacagaaa
    11881 ttgaagaaag agatccaaga ggggattcag aatcacggac gaacggttaa tcaaaaaggg
    11941 caaacgattt atgcatatga agtagatgga atgggaaacg ccacagtcat ggatgacagt
    12001 aatgtgccca atctgatcgc cgcaccttac ttgggatatt gttctgaaca agaggaagat
    12061 tatctgcaaa caagaagaac attgttgagt aaagaaaatc catatttcta tgaaggaaag
    12121 tacgcaaaag gaattggatc ctcccatacg ccagaaaaat acgtttggcc gattgcatta
    12181 gctatggaag gaatgacaac gtcagacaaa tctgaaaaag agcggatatt gaatttactt
    12241 gtagcaactg acgcaggaac acaccttatg cacgaaggat tcgatgtgaa caatcctgaa
    12301 aattatacga gagaatggtt ttcttgggca aatatgatgt tttgcgaatt agtcatggat
    12361 tactttgata ttagagtaga aaagtaggag gtatcgtaaa tgaaaaagaa agtatatatc
    12421 atttctcata gccactggga tcgtgaatgg tacatggctt acgaacagca tcacatgcga
    12481 ctgattgaat tgatagacga tctactagag ctatttgaag ttgatccgtc cttcaacagt
    12541 tttcatttgg atgggcaaac gattatttta gatgactatc ttcaagtaag gccagaaaaa
    12601 agacaggcaa tccaacaagc aatcaatgaa ggaaaattga gaattggacc attttatatc
    12661 ttgcaagatg attttcttat cagctcggaa tcgaacgtga gaaatatgct gattggcatg
    12721 gaagaaagcc gtaaatgggg aacccctgtc atgttaggct attttcccga tacatttgga
    12781 aatatgggac agacgcctca attaatgaaa caagctggta tttcagcagc agcgtttggc
    12841 cgtggtgtaa aaccaattgg ctttgataat caagttttgg aagcagaaaa ctattcttcc
    12901 caatactcag agatgtggtg gaaaggacct gatcaaacag caatctttgg tctgctattt
    12961 gccaattggt atagcaacgg gaatgagatt ccagtagaaa aagaagcagc tcttgccttt
    13021 tggaagcaaa aattagcgga tgcagaacaa tatgcctcta cgaaccactt attgatgatg
    13081 aatggtgttg atcatcaacc ggtacaaaaa gatatatcaa aagcaatcca tctcgcaaat
    13141 gaactgtttc cagattacga gtttattcat tctaatttta ctgactatct ggaagcagtt
    13201 caaaaagatg tgccagaaga cttaggctca gtagaaggag aattgacaag tcaagaaacg
    13261 gatggctggt atactttagc caatacagca tctgctcggg tttacttaaa acaatggaat
    13321 accaaggtcc agcgacagct agaaaatatt acagaaccac ttgcgacgat ggcttatgaa
    13381 gttagtggaa actatcccca tgatcaatta gattatgctt ggaagacatt aatgcaaaat
    13441 catcctcacg acagtatttg cggttgttca gtcgattctg tccatcggga aatgatacct
    13501 cgttttgaaa aagcagatga agttggaaag tatcttgcac aagattcctt agaacagctg
    13561 accgcagcaa tcgatacgac cggattccca aaagacagct ttccatttgt cattgtaaat
    13621 actgcaggaa tggataaaac tggtgaagca gaaataacca tcgaattaga aagaaaaaga
    13681 ttcgctgaag gaatacctga acaactatat caagagttag aaaatttgcc taaacgtaag
    13741 tatcatgtag aaacaaaaag tggggccact ataccagcta ttttatctga agaaactgtc
    13801 caatttggtt atgatttgcc gaaagaccgt ttccgtgtgc catatatggc aagaatgatc
    13861 aaagtgactt taccattaga aaacatgcca gcattttctt gggaaacatt cgcgcttgta
    13921 gaaggggaag cgaaagcaga agaaaaggaa acaatgattc atcagagtgg acgaatcatc
    13981 gaaaacggcc ctctacattt gacgattgaa aaaaatggta ccattacaat ggaagatcgc
    14041 aaaaacaaac gaaagttgaa taatctgcat atttttgaag atatcggcga tataggaaat
    14101 gagtatatct ttaaacaacc tttctgtgat cagccaatcc tatccagtaa caaagaaaac
    14161 tctgaagtaa aagtattagt agatacaccg gaaatagcaa aaataagtat tctacaggaa
    14221 atggaaatcc ctgtttctgc agacgagcgt ttagaaaaag aacagcagat ggttgtggaa
    14281 tttcgttatc gaaaagcaga acgatccaaa gaaaaacgaa tccttcaaat aaaaacaatc
    14341 atgacaatca gaaaagatag taaaaaaatc gactttgaaa cgactctcga caatcagatg
    14401 aaagatcatc gcttacgtgt cttgtttcct acaaagttac atgtggaaca tcacgaagca
    14461 gatagtattt ttgaagtggt gaagcgccca aaccatgtgt ctaaaagttg ggaaaacccc
    14521 acgaatccgc aacatcagca agcatttgtc aatattcatg atgaagagta cggtgtaaca
    14581 gtcggaaact ttggtttgaa tgaatatgaa gtaacagaag acggacaaat cgcagtgacg
    14641 ctacttcgca gtgtgggaga actaggcgat tggggatatt ttccaacacc agaagcacag
    14701 tgtctaggtg agcatcggtt caattacagc attgaactac atggaccaga agaaaaattc
    14761 tctacttatt tgcatgcata cgcagcacag attccttttt ccactcaaca aatcaaacat
    14821 catgaaggaa cattgatttc aaaacaacaa tatttaacga taaaaagtga aacgtttgca
    14881 attacagctt tgaaacgcag caagtttagt gataaagtag tagtaagagg tttcaatatg
    14941 tctagtcatt tagaaaaatt ggaaataact aaagacaatg gaaaaactgt catattgaat
    15001 ctgcttgaag aaccaacgaa gcaagcggtt gtcccaataa tccaacctta tgagattcgg
    15061 acaatcggat tcgaggagga aaacgaatgt atggcggaat agaagcaggc ggaacaaaat
    15121 ttgtgtgtgc ggtttcagat ggtggagaaa ttttaga
//
LOCUS       DAZKLA010000061        14992 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000061 DAZKLA010000000
VERSION     DAZKLA010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.062"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(559..1125)
                     /locus_tag="ACG85N_001668"
     CDS             complement(559..1125)
                     /locus_tag="ACG85N_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0224436.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1125..2081)
                     /locus_tag="ACG85N_001669"
     CDS             complement(1125..2081)
                     /locus_tag="ACG85N_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFQ0224437.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2081..2737)
                     /locus_tag="ACG85N_001670"
     CDS             complement(2081..2737)
                     /locus_tag="ACG85N_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0224438.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3107..5125
                     /locus_tag="ACG85N_001671"
     CDS             3107..5125
                     /locus_tag="ACG85N_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0224439.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5195..5401
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5552..7966
                     /gene="leuS"
                     /locus_tag="ACG85N_001672"
     CDS             5552..7966
                     /gene="leuS"
                     /locus_tag="ACG85N_001672"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFQ0224440.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8314..9867
                     /locus_tag="ACG85N_001673"
     CDS             8314..9867
                     /locus_tag="ACG85N_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFQ0224441.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10248..12032)
                     /locus_tag="ACG85N_001674"
     CDS             complement(10248..12032)
                     /locus_tag="ACG85N_001674"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224442.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12047..13768)
                     /locus_tag="ACG85N_001675"
     CDS             complement(12047..13768)
                     /locus_tag="ACG85N_001675"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224443.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13868..14629
                     /locus_tag="ACG85N_001676"
     CDS             13868..14629
                     /locus_tag="ACG85N_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0224444.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4882 a         2752 c         2963 g         4395 t
ORIGIN      
        1 ttcggaaaaa gctaatatag ctaaaattga aacatccatc tggaatttat cttttttact
       61 gctttgatca taaaagtctg catattcatc actgatattt tttctaataa attcttccat
      121 cataaaaatc acctcaaaac gattatacga acttacgttc ttgttgtaaa gcgatatttg
      181 aggggcaaaa aaggggcata atttgactga tattaagtta gctattgata tatgaatgac
      241 atacaaaaat ataaaatctt ttaaaaatgc ttatatatca atgtttttgc cattctttta
      301 tatatctgtt atatgtcaaa aaaatgtgtt caatttcaat ttgtatttga ataaagaggt
      361 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca atcaaaaaca gcttctcatg
      421 tttttgattt ggttggactt attaccgcag atataagctt ttgagcaccg tgaataaaga
      481 ggttatgaaa tcaacgctct aacctgagta ttagagaaaa aaacgcaaaa catatcattt
      541 atgttttgcg ttttttattt atcttttttt ctcaatacaa tataaaatag gcggctggtt
      601 cttttggttg ataaaccgat aattcagcac attatatgtt tcctgtggca gtttggaaca
      661 aaaagaatca accatatcca gttctttttc tccaccttca tgaccatagt agacgacaag
      721 gatcaatcgt ccgcgaggca caagtcgttt caatatttct tccatagctg tgcgtgtagt
      781 ctctggcata gtgatgattg ctttatcact ttttgggaga taccctagat tgaaaatccc
      841 tgctttgaga ttttgttgtt cagctatcac ttctcctaac gtttcgtggc cttgtagaaa
      901 caatgagaca cgttcttcta actgacgctg ttctagcctt tcacgagtag catcgattgc
      961 ttgttgctgg atatcaaaac tatagacatg accagttttt ccgatgtgtt ctgctagaaa
     1021 aaccgtatcg tggccattgc ccatcgttgc atctacgacg tgatcccctg gttgcaagat
     1081 ttcttgcagt aatgtatggc tgaaatgcaa agcagtttgc aacatcagac gctcaccttt
     1141 ttccgtacat catattttcc ttggaagcta tctctgcgaa gaagttcttc atcaattgca
     1201 ttcaagactt cccatttttt caagctccac attggtccaa tcaaggaatc ccacggcgca
     1261 tcccctgtca aacgatggat gatgatctct tttggtatca tttccagctg gtcgcaaatg
     1321 acattgacat agtcaggacg gctcattaat tgaaggcgac cttcatgata gtcccgcaac
     1381 attctcgttt tgcgcatcag atgcatcaaa tggagcttga ttccttggat atctgaatct
     1441 aaaatggtgc gtctgacatt ttctttcatc atttctaaac tttcgcctgg taatccgttg
     1501 atcaaatgcg tacaaacacg gatattatgt ttgcgtaatt tagccacgcc atctaaataa
     1561 gtttgataat catgtgctcg gttgatcagt tttgaagttt cttcaaaagt tgtttgcaat
     1621 ccaagttcta cccataaata catccgctcg ttcagttcag caaggtaatc aactacttca
     1681 tctggcagac aatccggtcg tgtaccgact gataatccta cgacacctgg taaattgact
     1741 acttgctcaa atcgttcttt gattatttca actggcgcat gggtgtttgt aaaattttgg
     1801 aaataaacga tgtattgttg gacatgaggc catttttgat gcatcatgtc gatttctttc
     1861 tggaattgga ttggaagagg gtcttcaggt gcaacgatca tgtctcccga accagataca
     1921 ctgcaaaaag tacaaccgcc atgggcaaca gtcccatctc gattcggaca gtcaaatcct
     1981 ccatcgatcg gcaccttgaa aattttcccg ccaaactcat ttcgtaaagc gtaattccaa
     2041 gaatgatatc ttttatttcc ttctgaataa ggaaaagtca ttaaaagtcc ttctttctgt
     2101 aagagttatc tatttgtttt cttttttctc tatacactgg ataagtcagg cacagccaag
     2161 aaagactgag taagtaaccg ccgatgatat cagtaggaaa atgaacgccc agataaatgc
     2221 ggcttatccc gacacttact ataataaaca tcatcagcaa ttgaagcaat gtgcgtaagg
     2281 ttcttttttg aacaaaagct ggaagcagaa atagtaaaga accgtaaaga atcatactgc
     2341 ctgctgcatg tccactaggg aaactgtaac ttgtttcaac tactaagtgc gccaatgaag
     2401 gacgctctcg catgaaaaat agtttgatca ccgggttgat gattccagca attacaataa
     2461 atccagaaac gaaccaaaat gtttctgccc tttttttctt ataccacatc cataaagccg
     2521 cacaagcaaa taaaatgatg acggtaagag gattaccgaa tttagtcata aagagttggt
     2581 agctgttcag atcaggataa gaagctctga caatgcctgt aaaccactga tcgaaaccag
     2641 aaagccacga cagatggaac tttacgatcc agcccaaaat gaaaaacagg acaagaaaga
     2701 agctaccagc cacctgaaat gatgttttat atttcataca attcctcctt tgaagcaatg
     2761 acttattata gcatttttgg tccagcgaaa aaaacggtaa agagataaaa ccaatgtatg
     2821 attcctcttt gaataaccaa tattcttata aaaaaaataa ctgtaatcgg aaagacattt
     2881 tttggtaatg caaatgaaat gttagggaaa taaaatcagt gtttttcgaa atccttttaa
     2941 acaagaacgt agacgtagga agaacttgaa aatgaacaga agttgttcat tacggtaaat
     3001 tacaatatga ttacaaaaaa caacaaagct cttaaataac ttttaataaa tattaagaga
     3061 agttataatt attaataata gataaattga agttggagga tagaagatgg aaaacattgc
     3121 aagaaaagaa cggagacgtt tagaacagac caaacgtttt cgaaaagcaa aacgaggggc
     3181 agcaatcgtg ggaactgcga tggtaggctg ttctgtcgct gccccattag tacagccggt
     3241 tcctgttcaa gcggatgaaa cacctgttca gttcggcgca agaatcaata cagcggcatt
     3301 tatcacagaa attgctactt atgctcaacc aatcgctcaa gccaacgatc tatatgcatc
     3361 agtgatgatc gcacaagcag ttgtagaaag cggttggggc agtagtgcgt tatctcaagc
     3421 accttattat aatctttttg ggataaaagg cagctatcaa ggacaaaccg tctacatgga
     3481 tacgcttgaa tttttaaata atcaatgggt aaataaaaag gagcctttcc gacaatatcc
     3541 ttcttttgct gaatctttca atgacaatgc ctatgtactt agaaatactt catttggcaa
     3601 cggttattac tatgccggta cttggaagag caacacgaaa tcctatacag atgcgacagc
     3661 ttgtttgaca gggcgttatg cgactgatcc tggttacgca ggcaaattaa ataatatcat
     3721 cgcaacttac ggattgaccc aatatgatac accagctagc ggaaacgcag gcggtggagc
     3781 gactacaggt ggcaataata ctggaagtgc aggtagtact ggcggttcaa gcaattctgg
     3841 taatacaaac tcaggttcag caacttcgac cacttatact gtccaatctg gtgattctgt
     3901 ttggggaatc tcaaataaat tcggtatcac aatggatcaa ttgatccaat ggaacaacat
     3961 ccaaaacaat ttcatttatc caggacaaaa actgacaatc aaaggtagcc aggaaaatgg
     4021 ctcttcgacg aataattccg gtaacaatac caattcttca ggaaatgctg gtacatcaaa
     4081 cggaggacaa acaactggtg cgaaatacac tgttcaatct ggtgattctg tttggaagat
     4141 ctcaaatgat cacggaatta cgatggctca attgattgaa tggaacaata tcaagaacaa
     4201 tttcgtttat ccaggacaac agctaatcgt atcaaatggc ggttcttcta atgctggagc
     4261 aaatactggc tcaaatactg ggaatacaag tggctctagt tcgtccagct caagttcttc
     4321 taatacagct tcaggaacga aatatacagt taaagccgga gactctgttt ggagcgtctc
     4381 aaataaatac ggaattacga tggctcagct aatccaatgg aacaatatcc aaaataactt
     4441 tatctaccca ggacaacaac tgactgtttc aaacagtgga tcaacaaaca gcacgtcagg
     4501 taatacaggt tcgtcaacca atacaaataa cagcagttca gttaatactg gcagcagttc
     4561 gtctcaaggc acttataccg tgaaagcagg tgaatctgtt tggagcgtgg caaataaaaa
     4621 cggcatcacg atggatcagt taatcgaatg gaataatatc aaaaacaact tcatttatcc
     4681 aggacagcaa ttgatcgtta aaggtggaac ttcggcaaat acaaatacag gttcaactac
     4741 ttctgcagcg aaaccaaata caccgaatac gtctgctacg acaagtacat catccactgg
     4801 taatacgatg tataccgtga aagcgggcga gtcagtttgg agcgtagcga acaaacatca
     4861 tatcacgatg gatcagttaa tcgaatggaa taatatcaaa aataatttca tctatccagg
     4921 acagcaagtg attgtgaaga aaggttcatc tcaatcaaca aaccaacaat cagttactgg
     4981 ttcgaaaacc tatactgtga aatctggaga atctgtttgg agtgttgctg acagtcatgg
     5041 aattacaatg gctcaactaa tcgaatggaa caatattaag aacaacttta tttatccagg
     5101 tcaaacgttg attgtagcta aataaaatag ctgttcgaag aaataacgat tgaattccac
     5161 aaaaatattt gttactataa ggctacttaa atatgacaac acttgaaaag gaagtagtaa
     5221 aaagactgaa gtttcagaga gcgcatggct ggtgagaatg tgcacggatg cttttgaact
     5281 cgcctttgag ttgcccttgc tgaatacccg agtaagcaag cggcggtgac ggccgttaca
     5341 acgttcgagg ccttttcact aaggtaaaat taggtggtac cgcgtagatt acgtcctata
     5401 aaaagagcaa tctttttata ggattttttt atttcttttg tgaagtttga gaaaacaaag
     5461 caaaaggaaa taaaaataaa cagattctgc cggttttttc gttatattta taatagaaac
     5521 aagtgttttc aatagaacag gaggacagca aatgagttac aatcataaag aaattgagaa
     5581 aaaatggcaa aaatactggg caaaaacgaa tactttcaac acccatgatg atcctgaaaa
     5641 gccaaaattt tacgcactgg atatgtttcc ctatccatcc ggtcaagggc tacatgtagg
     5701 gcatcctgaa ggttatacgg caacggatat cctttcacgt gtgaaaagaa gtcaaggata
     5761 caacgtgctg catccaatgg ggtgggatgc attcggtctg cctgctgaac aatatgcgct
     5821 agatacagga aacgatccag ctgagtttac ccaaaaaaat atcgaaacat tccgacgcca
     5881 aatcaattct ttaggattta gttatgactg gaatcgcgaa gtgaatacaa ctgatccgga
     5941 gtattataaa tggacacaat ggatctttac gaagctttat gaaaaaggtc tagcttatga
     6001 agcagaagta gcggtaaact gggtacctga attaggaaca gttatttcaa atgaagaagt
     6061 gatcgatggg aaaagtgaac gtggtggcta tgatgtagtc agaaaaccaa tgcgccaatg
     6121 gatgctgaaa atcactgctt atgctgatcg cttattggat gatctagaat tagttgattg
     6181 gccagaaagc attaaagaaa tgcaaagaaa ttggatcggt cgttcagtag gagcaaatat
     6241 tgaatttaaa gtggctggta cggataaatc ctataccgta tttacgacac gaccagatac
     6301 tctattcggt gcaacttact cggttcttgc acctgaacta gatcttgtta gagaaattac
     6361 gacccctgaa caaaaagctg ctgtagaagc ttatatcgaa gagacagcta aaaaatcaga
     6421 tctaaagaga acggatcttg caaaagaaaa aacaggagta tttactggtg cttatgcaat
     6481 caatccagtt aatggcaagg aaatcccaat ctggatcgcc gattatgtat tatcttcata
     6541 cggcacagga gccatcatgg ctgttccagc acatgatgaa cgtgactttg aattcgctca
     6601 aactttcgga ctagaaattc ttccagttat cgaaggtgga gatgttgaaa aagctgctta
     6661 cacagaagat ggtacgcaca tcaactctga atttctagac ggcatgaaca aacaagaagc
     6721 aatcgataaa atgaatgaat ggctagaaga aaatggcgta gggaaaaaag aagtcagcta
     6781 tcgcttacgt gactggctat tctcccgtca acgttactgg ggtgaaccaa tcccaatcat
     6841 ccattgggag gacggaacag tcacagctgt cccagaagaa gagctaccat tacggttgcc
     6901 taagacaaaa aacatcaagc caagcggtac tggagaatct ccattagcaa atatcgagga
     6961 gtgggtcaat gtcgttgatc ctgtaacagg taaaaaaggc cgtcgcgaaa cgaatacaat
     7021 gccacaatgg gcaggaagct catggtatta cctgcgttat atcgaccctc ataacaaaaa
     7081 agaactagca gattatgaaa agttgaagcg ttggctacca gttgacatct acattggtgg
     7141 agcagaacat gcggtacttc atttactata tgcacgattc tggcataaat tcttatacga
     7201 tatcggtgtt gttcctacaa aagaaccatt ccaaaaacta tacaatcaag gaatgatctt
     7261 aggcgaaaac aacgaaaaaa tgtcaaaatc tcgtggtaat gtagtcaatc ctgatgatgt
     7321 ggtcgaaact tacggtgcag atacactgcg catgtacgaa atgttcatgg ggccattaga
     7381 tgcttcgatt gcttggagcg aaaatggctt agaaggaagc cgtaaattct tggatcgtgt
     7441 atggcgtttg atcgttgatg agaacaataa aatgcgtgac cgtatcacta cattgaatga
     7501 tggcaagctt gataaagtgt atcatcaaac agtgaaaaaa gtgacagaag actatgaaaa
     7561 tcttcatttc aatacagcaa tctcccaatt gatggtcttt atcaatgaag cctataaagt
     7621 ggacgctctg ccttatgaat atatcgaagg atttgttcaa ctactcgcac cgattgctcc
     7681 tcacatgggt gaagaactat gggcaatcct tggaaatgat ggtggtattt cttacgcacc
     7741 atggccaaca tacgatgaag cagcacttgt tgaagacgaa gtagaagttg ttttccaagt
     7801 aaacggtaaa gttcgtgcga aaagcaatgt tccccgagac ttaggaaaag atgagctaga
     7861 aaaagcagca ttagccaatg aaatagtgca agaatatatt gaaggcaaaa cagtcagaaa
     7921 agttattgcg gtaccaaaca agttagtcaa tatcgttgct aattaattgg ctctttgtca
     7981 ataaggactg atgagttgtg caaaatcaaa tctgggccag aatgaacttc attctgtctc
     8041 agattttttg ttatttcaaa ttgattaatt gattgatcaa aaaggttttg attttcatgt
     8101 tctcaaacac gttcaatgtc tttttcttct gaatggtctt gtaagagatc tctgtaatca
     8161 tcaaattttt gtctattaat cttagtattt tttgatgtat aaactacctg aaattgagag
     8221 tacataccaa attcataaat tttttattag tgggactagt gattgtatgg attttgacgc
     8281 ataatgaatg aagtatgaga aaaggagaga caaatggatc aagaactatt tattatcgaa
     8341 gtattatcag ctaaacaaaa aattatcgag aaagcgaaaa aaagagcgaa aagaataatg
     8401 ttcaagcttt taagcttagt atttttctct ggtttgcttt ttttcggagc tgtatcgttc
     8461 ctatatcgcc atatgagtgc agtagtccgt tcagctgaag ggatgcaaaa attaaataat
     8521 ttgtatcact tttcgtgggg agttggcgga ataacaattg ttgtgcttat tgtttttctt
     8581 ctacttattt ggctccgtta ccatcaattg tcgcgtcatt tcgatcaatg gcttagtcaa
     8641 agagcacaac agtttttaca taataaacca acttctcaag atgatgacta tctttatttt
     8701 gagaagactt cgacgagtca agcttttcgg atacaaaaaa gtgattgcca gttattgctg
     8761 agatcacttg atgatttacc tatctactta ggcgaaagca aatcaaaatt cggaatgaac
     8821 acattccata tttttttact agaaaacgaa cccagatggg aaaaaagcca gtcttcgcct
     8881 ggtggatacc tgacaactaa gatgagtaca ttagtcactg gttttttcct cattctttta
     8941 gctggaacaa tctattatgg gagatacact cagccaaaaa tgtcttctca tataaatgct
     9001 gcttctcaca tgacaatgaa ctcaactaac gatacgcata tacaaaagac aaatgaaggg
     9061 ttgttagctg atcctaagca aaaggtaaat ttgacaacac aaaagataaa tgatctcaaa
     9121 cttgatacag agacgcactc tctctatatg acaacagata gtggtgctaa ttgggtattt
     9181 gtgccgattg attcgcaatg gttgcgtttt ggcgaataca ccctgacaac ggggctgatt
     9241 cctgaaggtt attggatgga caacacatat gatatttccg ccgatttctc atggtttatc
     9301 tattcttctg gggatgatgt acgtctgctg acttcaagag ataatggtaa aacatggcaa
     9361 acgtctatta tcacccaatc agttcagccg atacgttatc gaaaagcggt attttatggc
     9421 aacgatggtc tatttatctg tacatcagcc agtgggatgt ctgcagaaac tttgtacatt
     9481 tacactacta tggatggcgg aatcacttgg ataaaaagtg gaagtacaat gatcaatcag
     9541 ccagtacaaa atgtcagctt cgttacaact tctttaggtt ttgtttcgac acgtgaaagt
     9601 ttgttttata caaacaacgg aggacgctca ttcaaagaat cagttctgtc gattccggaa
     9661 aactatcaga caggggggat agatcttttt caatcaccaa atgaagttac tcaaataagc
     9721 gcgaatacgt tagagacaaa attttatttg ttgaaaaaag gaccaatcga tgcagggaaa
     9781 atgtttgcct gcctcttccg ttcaactgat aacggggaaa catggcaatt cgttcagcaa
     9841 ctatcgcaag tagaacaagg agattaaaga agagagattg tgagcctgac gttcagcacc
     9901 gagtcacaag aaacaaattc aaaaaacagc ttctcatatt tgttgaatag tgtaactcgt
     9961 gattcggagt tgacaagagc aacaagaagc gattttgaag tatagcttct cgtattttac
    10021 aagatcgtgt cttattgccg ataggttgtc tcttgaacac cgtttattcg taaaaaggga
    10081 ttgtgacaag tcttttgtca ctacttaccg aacgaaactg aaggagctat ccaaaaagta
    10141 tcattagtat tattctttgt gtttctagtg gcaccactag tacatttaaa aaagctatcg
    10201 atcatggact aatcatacat gatcgatagc tttttagata atgtttgtta cgctatctga
    10261 aattgactat tataaagact ggcataaagc gttggtgatt ggagtagttc agaatgtgtt
    10321 cctttttcga cgatatcgcc attcttcatt acaaggatca tgtctgcatt ttcaatcgtt
    10381 gacaaacggt gtgcaatcac aaaacttgtc ctatcttttg tcacagcttc cattgcttgc
    10441 tgaatcaatg cttctgttcg tgtatctacg ctagaagttg cttcatctaa aataacgaca
    10501 ggaggattcg ctagaatgac tcgagcaatt gtcaataatt gttgctgtcc ttgagataac
    10561 gtgccgtttt cgctagaaat gatcgtatca tatccgtctg gcagtgttcg aataaagtga
    10621 tcacattgtg cgatttcagc cgcttcgata atttgttctc ttgtggcatc cattttcccg
    10681 taggcgatat tatcagctac cgttccttca aacagccagg tattttgtaa aaccatccca
    10741 aaagaacgac gtagttctcc acgtgaaagc ttggtaatat caagaccgtc gaattcgatc
    10801 tttcctttat tcagttcata aaaacgcatc agcagattga ctaaagtcgt ttttcctgca
    10861 ccagttggac caacgatcgc taccgtttgt tttggcttta ctgaaaaatc gacttgattc
    10921 atcaatattt tatctgatgt gtaaccaaac tgtacatttt tgaaagctat ttttcctttc
    10981 ggttgttcta aaagtcttgc atctggaagg tctggaattt catctgtttc atctaatatc
    11041 tcaaatatcc tatcgattgc tgccattgcg gattggatcg agttgatcac ataagaagcg
    11101 gtagaaattg gttcagaaat ctgattgatg tactgaagat aagcttgtaa gaagccgatc
    11161 gtcacaccac ctgatagaac aagcatagca ccgaataccg cacttatgat gaaggcgagt
    11221 tgattaatga aacgaatggc cggatagata gcacagttca taaattgagc acgtttaaaa
    11281 gcgttgtatt gctttttatt tacttcgtgt atagcagctt ctgcatgagc ttgctgattg
    11341 aatgttttga ccactagatt accagctaag atttcttcca ttcgattatt taaaacaccc
    11401 agttcatact gattctgttc agaaatctgt ttgtttttgt tcgcaatctt agttgtaaca
    11461 attgaactgc caacgatcag caataagacg agcaaagtaa gcttgctttc gatataaaac
    11521 agcatcagac cagcagcagc tattgttacg acagaagtaa aaaattgatt gatacctgtc
    11581 aataacactt gagaaagttg attgagaccg gtagttgtcc gactaatgat gtcacctact
    11641 tgatgattgt caaaaaacga catcggtaat gttttgaatt tttttgtgat ttcttttcgc
    11701 accctcaatg tgatcttttc gcttaacgct gccattgtcc gttcctgaat aaatgaagtg
    11761 atgctgctta agactgaaca gccgatcagc aataagacag ggaacaacaa tgcttgtttg
    11821 atatcactga aagaaagctg tccaatacct gtttttctta tttctgataa gagatcatcg
    11881 atcccgattc ccatgatcaa gggcatggct acgactaata ggttcccgaa taaactgcat
    11941 agaaacaatc cgtaaaaata tttatgttca ggtttgatca ttttccaaaa acgtatcaaa
    12001 gcacgcgtgt tcaacgtttt tttcttttcc atcgtatccc ttctttctat attccttgtg
    12061 attttgcaaa ctcctgataa gtttggcaat tagacagtag ttcttcatga gtaccttgtc
    12121 caacgatttt tccttcatct aaaacaatga tgttatctgc ttgttggatt gtactgaggc
    12181 gttgagcaac aatcacgaga gttttctccg acatgtgttt atgaagggct gctcttaatg
    12241 ctgcatctgt tttatagtct aaagcggaga aactatcatc gaatacatag atctctgctg
    12301 gtttgatcaa cgctcgagca atacatatcc gctgtttctg gccccctgaa taattagaac
    12361 cgccttgagc aacaaagctg tccaatccgt caggaagggt gtccactagt tcttctaatt
    12421 gagcgatctt gatcgcttta gacaactctt ctgttgaggc atattcattt cccatctgca
    12481 agttgcttaa aattgtcccg ctgaataaga aagccttctg agggacataa ctgatgtggg
    12541 agcggatctg ttcttgagtc atttgagtaa gaggaatacc accaagggtt acttttccta
    12601 tcgtagggtc actaagtctc aagagaagtt tcgccaaact actttttcca gatccggtcc
    12661 cgccaacgat cgctgttgtt tttcctttag gtattgtgaa atcaatatct tctagaactg
    12721 gatcagagat ttcagtatat ttaaatgtga catgttcaaa agcagctgca tttgtctgat
    12781 caaaggttgg ttcagctact gttttgatcg tctcttcctc aatttcgatc tctgcttgca
    12841 gtatttcttc tatccgtcgt aatgaggcaa acgatttagg gataatgacc aataccatag
    12901 ctgcgatcat caagtaagca agtgtcagca tagagtattc gactacagca gtcacgacac
    12961 cgatttgtaa tgctccttct gctacaagat ttccgccaaa ccacaaaata gaagcataaa
    13021 cgattcccat gagcaaaaaa gcaattggag tgacccaagc aaatattcgg ttcactttga
    13081 tcatttgatt agcatagtca gcaaagctct tattcgttcg cttttcttca tatgattgat
    13141 tatcaaaggc acggatcata ttaatcccag taaagaattc tctcaatgtg atggtgatcc
    13201 gatccatttt tggctggata ctcaaagata aaggtgcccc tcgtttcatc agtcgataaa
    13261 caatccatac ataaaaaaca acagagatca atgtaaccca tgttagtaat agagaataac
    13321 gaaacatcat gagaatggtg aaaacgcaaa taaccggtgc tggtaagatc atttggcaaa
    13381 ataaaacaat catttgctgg acattatcga catcattcgt cattcttgtc aaaagagatc
    13441 cagaactaaa acgactgata tccttcattg acagctgttg gaatttacgg aaatatttct
    13501 cccgcgtttg gaatccaaac ctagcggaag ccaatgctga aaagtaacta gaggcaactg
    13561 cagcgattgt tcctagaaac gctacaagca gcatttcaac tccaattttt tttataactg
    13621 aggcttcgcc acttgctata cctgtgtcga tcagctttgc tactaatttt ggtacaccta
    13681 atgtaccggc gatttctaaa cagatcatca caaaggtaag gagagcgagt cctttgtttt
    13741 tctgtaagaa tgtttttaag atagacatat tattcctcct tttttcctga tacttttcga
    13801 ttctatacct tcaagtaact tgaaggtcaa tagaaaaaag aataaactga aaaagaggtg
    13861 agaaaaaatg aaaaaaatgt atacgattgg ccaagtatcg gaactatttg atttgccaaa
    13921 atcaacgatc cgctattggg atgaacaagg attaatccgt tcaagtagac aagaagagaa
    13981 tgattatcga ttgtttgata tcgatgatat ttttatgctt tatgatatcg ttttttatag
    14041 aaaattagat ataccgatca aacagatgaa gaatttatat ggaaaaacat tgacggagtt
    14101 atatgagaca cttgatgaaa cagaaagaag aatccataaa gaactggtgg tgatgaaaca
    14161 aaaacaaaaa gagataaggg agcaaaaaaa acagttgaaa ttgatgatcg atacaaacga
    14221 ggaagaattt cctgtagaag agatcccttt tgactgtatg atctcaacgg agtttgaaga
    14281 tattgtggaa atcaaaaaat ttctacctaa ctattcaagc tttgggatga tgagtatgcc
    14341 agcttccagt tcacagacga tgtatggatt ttttatcgac cctagtgaag tacatttatt
    14401 tactcaagac gtgatttggg agaaaaaaga tacggctgtt tatcgtcgat tcttattgaa
    14461 atccgagatg aaccatgcgg aacgtaataa tattttagaa atcagagaaa gaatgtatga
    14521 aaaaggatgg aaaacaggag aagtgatcgg ccagtatcta ttgacgaata cggatgaaaa
    14581 caatatcaga actgaatatt atcatgcatg gatagaaatg aaaaaatagg aaatatgtgt
    14641 ttaagataac tcgaaaatga tggttgtttt agttataaag aaaaattttc tggttcgatt
    14701 acgggtttaa tgtgggggat tattagtcgt ggatagatag aattttagtt ttttagagga
    14761 aatatgtctt taaaaatgga atggttgcaa ttcttagcga tatatacgat gtaattgtcc
    14821 tcttaaaaaa gtatggaaaa ataattgtat tgggtgttat gtataattca tggtgaactg
    14881 agactaatga gaagaaaaat tcatattgtt atgagtgaga ttgtatcaga tgagaatgat
    14941 aaattaaata ggtgcataaa agcattacac ttaaaataca ttgttgctta ca
//
LOCUS       DAZKLA010000062        14622 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000062 DAZKLA010000000
VERSION     DAZKLA010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.063"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..813
                     /locus_tag="ACG85N_001677"
     CDS             <1..813
                     /locus_tag="ACG85N_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFQ0224445.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            810..1610
                     /locus_tag="ACG85N_001678"
     CDS             810..1610
                     /locus_tag="ACG85N_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFQ0224446.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1771..3039
                     /gene="rseP"
                     /locus_tag="ACG85N_001679"
     CDS             1771..3039
                     /gene="rseP"
                     /locus_tag="ACG85N_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFQ0224447.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3107..4816
                     /locus_tag="ACG85N_001680"
     CDS             3107..4816
                     /locus_tag="ACG85N_001680"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFQ0224448.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5028..9386
                     /locus_tag="ACG85N_001681"
     CDS             5028..9386
                     /locus_tag="ACG85N_001681"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFQ0224449.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9531..10004
                     /gene="rimP"
                     /locus_tag="ACG85N_001682"
     CDS             9531..10004
                     /gene="rimP"
                     /locus_tag="ACG85N_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFQ0224450.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10027..11202
                     /gene="nusA"
                     /locus_tag="ACG85N_001683"
     CDS             10027..11202
                     /gene="nusA"
                     /locus_tag="ACG85N_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFQ0224451.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11224..11517
                     /locus_tag="ACG85N_001684"
     CDS             11224..11517
                     /locus_tag="ACG85N_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFQ0224452.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11514..11825
                     /locus_tag="ACG85N_001685"
     CDS             11514..11825
                     /locus_tag="ACG85N_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFQ0224453.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11838..14144
                     /gene="infB"
                     /locus_tag="ACG85N_001686"
     CDS             11838..14144
                     /gene="infB"
                     /locus_tag="ACG85N_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFQ0224454.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14171..14518
                     /gene="rbfA"
                     /locus_tag="ACG85N_001687"
     CDS             14171..14518
                     /gene="rbfA"
                     /locus_tag="ACG85N_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFQ0224455.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4981 a         2598 c         3254 g         3789 t
ORIGIN      
        1 atgttacgtt tttttccgca aaaaaacaaa tatattcaag aaaaaagcga atttcaattt
       61 aataccgatg gaccaattcc tcagcacatt gccatcatca tggacggcaa tggtcgttgg
      121 gcgcaaaacc gtcgtttgcc acgagttgct ggccataaag aaggcatgga aacagtaaag
      181 aaagtcacaa aaaaagcatc aaggcttgga gtgaaagtgt tgacgcttta tgccttttcg
      241 actgaaaact ggaagcgacc aaaagatgaa gtaagttttc tcatgcaact acctgtcgac
      301 ttctttgata cgttcgtacc agaattgatt aaagaaaatg tcaaagttca tgtcatgggt
      361 tatgaaaacg tattgccaga acatacgcaa gatgctgtaa gacgtgcaat tgagcaaaca
      421 aaaaacaata ctggaatggt attgaatttt gctttgaatt atggaagtag agcagagatc
      481 gtgactgctg tccaagaaat tgccgaagaa gtagcaaaag gagaaataca cgcagaagaa
      541 atcgatgatg aattgattgc taagcattta atgacaggtt tcttgcctaa agagttacaa
      601 gatcctgagc tgatgatccg tacaagtgga gaagagcgaa ttagtaattt tctgttatgg
      661 cagatcgcat atagtgagct gtattttaca aaagcgcttt ggcctgattt tgatggtgcg
      721 catcttgaag aagcaatcgc ctcttatcaa aatcgagacc gtcgattcgg cggtgtaaaa
      781 aaaacagaag atgagaaagg ggatcagtca tgaaacaacg tgtgatcaca gcagctgtgg
      841 cgctagtctt gtttattccg attatatgga tcggaggcat agcggttgag cttacagcag
      901 caatcttagc ggtagtagga gtttacgagc tgttccggat gaaaggattg acactcttaa
      961 gttttgaagg agttttatcc gccgttggtg cagtgttact tgttttgcca aaagaaagat
     1021 ggtttttctt tcttccagag aatgtaagta catttatgtt attttatctt atagttatga
     1081 ttcttttagg aacatcagtt atttctaaaa atacgtatac gattgatgaa gcaggttttc
     1141 ctgtgcttgt cagcttgtat gtgggaatag gtttccagaa ttttgtcaat gcacgaacag
     1201 aaagcttggc tgttttgata tttggtctat ttattgtttg ggcaactgat atcggagcat
     1261 atatggttgg tcgacgttat ggtcagcata agttaagtcc tgatatttcg cccaacaaaa
     1321 cgattgaagg tgcgctcggt ggtatcggta gtgctgttgt agtcgcattt ctttatttat
     1381 tgctataccc agcaaaagaa ctattcaatc atggtatgtt tgtcatgctt ctgatgacga
     1441 ttattttttc agcagtcgga caatttggtg atttggtcga atctgcaatc aaacgtcatt
     1501 atgatgtgaa agactcggga aatatcttac caggccatgg tggaatctta gaccgtttcg
     1561 acagtttgct gtttgtattt cctatcatgc atttatttgg cattatttag taagtatacc
     1621 tgcgtttctt tgtgaatttc ataaaagacg caggttttta tttttcatta tattgatact
     1681 attggtgtct ttggtatact atggtaaaat tatagtgttt tatcgttgtt ttataaaaaa
     1741 cacaaaaaac aagtaaagga gaaaaaagct atgaaaacga ttctgacatt tatcatcgtt
     1801 tttggtatat tagtgattgt tcatgagttt ggtcatttct tctttgcgaa acgatcagga
     1861 atcctcgtcc gcgaatttgc tatcggtatg ggtccgaaaa tttatgggca tcaagcaaaa
     1921 gacgggacaa catatacgct tcgcctatta cctattggcg gttatgttcg aatggcaggc
     1981 aatggggatg atgaaacaga aatggcacca ggaatgcctc tttctctttt actaaattct
     2041 gacggcatcg tagagaagat aaatttaagt aagaaaatcc aattgacaaa tgctatcccg
     2101 atggaattga gccgttatga tttagaagat gagctgacta tcactggtta tgtgaatgga
     2161 gacgaaacag aagtagtaac ctaccctgtt gatcacgatg cgacgattat tgaaaacgac
     2221 ggaacagaaa tcagaattgc gccaaaagac gtacagttcc aatcggctaa attgtggcag
     2281 cgtatgctga ccaactttgc tggacccatg aataacttta ttttggcaat cgtgttattc
     2341 atcattctgg catttatgca aggtggtgtt caagtgacga acactaatcg cgtaggagag
     2401 atcatgccta atggagctgc ggcagaagca ggcttaaaag aaaatgatga ggtagtcagt
     2461 gtggacggaa aagaaatcca ttcatggaat gatctgacaa cagtcattac gaaaaatcca
     2521 ggtaaaacat tagactttaa gatcgaacga gaaggtcagg tgcaatctgt cgacgtgaca
     2581 ccgaaatctg tagagtcaaa tggcgaaaaa gttggccaac ttggtataaa agccccaatg
     2641 aatacaggtt ttatggataa gatcattgga gggacgcgtc aagctttcag cggttcattg
     2701 gaaattttca aagccttagg atcactattt acaggtttca gtttagataa attaggcgga
     2761 cctgtcatga tgtaccaatt gtcttcagaa gcagcaaatc aaggtgtaac aactgttatt
     2821 agtttgatgg cgcttctttc aatgaatctc ggaatcgtca atctgcttcc gattcctgcc
     2881 ttagatggcg ggaaattagt cttgaatatt tttgaaggga ttcgcgggaa accattaagt
     2941 caagaaaaag aaggaatatt gacattagct ggctttggat ttttaatgtt gttgatggtg
     3001 ctagttacat ggaacgatat tcaacgattc tttttctaga aaaagttgac aaatagggta
     3061 taataacaaa cgataactat tttgaggaaa taaaggagta tatagaatga gacaatcaaa
     3121 aatgttgatt ccaacattac gcgaagttcc aaatgatgca gaagtattaa gccatcaaat
     3181 cttgctaaga gctggatata tccgccaagt atcagcgggt atttattcat atctgccatt
     3241 agcaaatcgt gtattggaaa aactgaaacg cattatgcgg gaagaatttg aaaaaatcgg
     3301 agcggtagaa atgctgatgc ctgccatctt gccagcagaa ctttgggaag aatccgggcg
     3361 ttacgaaaca tatggaccaa acttgtatcg tttcaaagac cgcaatgatc gaaaaatgat
     3421 ccttggacca actcacgaag aaacttttac tgatttgatg cgtaatgaaa tcaattcata
     3481 taagaaatta cctttaaatc tttatcaaat ccaacctaaa tatcgtgacg aaaaacgacc
     3541 tcgcttcggt ttgcttcgcg gaagagagtt tatcatgaaa gatgcatatt ctttccatgc
     3601 aacggaagaa agtttggacg agacatataa agattacgaa aaagcctata ccgaagtatt
     3661 caagcgttgt ggcttagaat ttcgctcgat catcggagac ggcggcgcaa tgggcgggaa
     3721 agactcaaag gaattcatgg cgatctcaga aatcggggag gatacgatct gttattcgac
     3781 tgaaagtgat tatgctgcaa acttggagat ggctacaagc tactatgtat caaaaaaatc
     3841 gcatgaaaca cagcttgaat tggagaaagt agctacacct aacgtaaaat caattgacga
     3901 agtagcagat ttcttttctg ttgaacctca aaaaatcatc aagtctgtat tgttcattgc
     3961 ggatgaacaa cctgtccttg ttttggtacg tggagaccat gaagtcaatg acgtaaaact
     4021 gaaaaacttt ttatctgctg actttttaga agaagcaaca gaagaagaag cacaaaaata
     4081 tctaggtgct gagtttggtt ctgtcggacc agtcggtgtt tcagatgacg taaaagtata
     4141 tgcagaccgt tatgtccaag atttagcgaa tgccattact ggagcgaacg aaacaggatt
     4201 ccactataaa aatgtgaatc cagaacgcga tttcactgtg ctttcttatg aagatcttcg
     4261 ttttgtacaa gaaggagatc cttcaccaga caacaacggt gttcttgcct ttactcgagg
     4321 aatcgaaatc ggacatatat tcaaaatcgg cactcgttac agtgaatcta tgggagcaac
     4381 tgtactagac gaaaatggac gagaaaaatt tgtgattatg ggctgttatg gtatcggtgt
     4441 aagtcgttta ttatcagcaa tcgtagaaca acattcagat gaacaaggaa tcaattggcc
     4501 aaaaggaatt gcgccatttg atctgcatat cgtccaaatg aatctaaaag acgaatacca
     4561 aacaagcctg acgaatgaac tagaagaagc gatgacaaaa gctggctatg aagtactggt
     4621 tgatgacaga aatgaacgcg caggagtgaa attcgcggat tctgatttga ttggctgtcc
     4681 aatcagaatc actgtcggta aaaaagcagt ggatggaatc gtagaagtga aaatcaagaa
     4741 aactggcgaa atggttgaag tacgtaaaga agaattggcg aatactttac caatcctctt
     4801 gaaccaaggc gaataataag cgttaaaaca cagaaaataa aaatagctga atcagctgtt
     4861 tttgacaatc tgatgattcg cttttaaaat tggaactgcg atttgaatcg cagttccaat
     4921 tttttaatgt aaaactcaga aaaaacgagt atactaaaag gtactgaaaa agaagaggat
     4981 aagatgcgat caattgcatt gtttctcatc agaaagggcg tatatctttg tcagaaaaac
     5041 gagatttatt tgaaaaactg ttggaacaaa tccaactaga ggaagcagaa aaaaatcatc
     5101 cactgcttac tgctggtgaa atggatagtg tagtggtcca tcgtaaatcg cgtttatggg
     5161 aatttacgct acatttttcc aaaattttgc ctattatgct atatcggtca ttgactcagc
     5221 atctgactat cgcttttaaa gatattgcac aagtcaaact gaatatctta gcagatgacc
     5281 agacattcga tgaacagctc ttgcaagatt actgggccca agctttggaa aatcagcaat
     5341 gcgatacacc actagcacag caagtattga aaacgcaggt acctgtgata aaagatcgta
     5401 aagtcatctt gccaatcgac agtacaggag ctatttctta tttgaagcaa caatacttgc
     5461 cattagtaga agagttgttc gtcagttacg gattcccaaa atttcgtatc gaaccagaag
     5521 tggatgagca gcaagcggaa cgagtattga aactttttga agaacgcaaa caagaacagg
     5581 cagaagcctt tatgaagcaa gcagctgaaa gtctagttgt ccacgaacaa aagaaaaagg
     5641 aaagaaaaga gcaatcgcca gctcttgaag gacccatcca catccagcta ggacgaaata
     5701 ttccagccga tgaaccaacg acaccaatga tcagtatcgt tgaagaagaa cgaagagtca
     5761 cacttgaggg gtacgtattc gacaaagaag tcagagaact acgttcaaaa cgcaagatct
     5821 tgactttgaa aatcacagat tatacttcat catttatcgt caaaaaattc tcgaacggcg
     5881 aaaaagatga acaagtattt gatgcgatca gtgtcggtag ctggttaaag gtacgaggaa
     5941 gtattcaaga agatacgttt gtccgtgatt tggtcatgaa tgcgcaagat atcatagaag
     6001 tcaaacatac acctcgcaaa gattatgctc ctgaagggga aaaacgagta gagcttcatg
     6061 ttcatagcaa tatgagtacg atggatgcaa caaacagcat ctctgatctt gtggcacaag
     6121 cagggaaatg gggacaccga gctatcgcca ttacagacca cggtggtgcg caagcctttc
     6181 cggaagcaca tagtgctgga aaaaaagcag gtgtgaaaat cttatacggc gtggaagcga
     6241 atgtcgtaga tgatggtgtc ccaattgctt ataatgatgc acatgaagca ttgagtgaag
     6301 ccacatatgt cgtatttgac gtggaaacga caggattgtc tgcagtatat gacacgatca
     6361 tcgagctagc cgcagtcaaa atgtataaag gaaatgtgat cgaaagcttt gatgaattta
     6421 tcgatccagg acatcctttg tcacgaacaa cggtagatct gactggtatc acggatggaa
     6481 tggttcgagg atcaaagtcg gaagaagaag tattgcggat gttccttgaa ttttcaaaag
     6541 atacaatttt ggtagcccac aacgcggctt ttgatatggg gttcttgaat actagctacg
     6601 caaggtatgg tattcctgaa gcggccaatc cagtcattga tacgttggaa ttagctagat
     6661 atctgtatcc tcaattcaaa cgtttcggat taggtgtgtt atctaaaaaa tttggtgtca
     6721 gcttggaaca gcatcaccga gcaatctatg atgctgaagc aactggtcac ttagcatgga
     6781 tctttgtgaa agaggcaatg gacaatcata acatgcttta tcatgatcag ctaaatgagc
     6841 atatcggaga aggagattct tacaaaaggg ctcgaccttt ccatgtaacg attttagcaa
     6901 aaaatcaggc aggactgaaa gatttgttca aactgatttc aatgtcgaat gtcgaatact
     6961 tcgaacgcgt cccacgaata ccacgctccc aattgaaaaa gatgcgtgaa aatctattga
     7021 tcggctcagc ttgtgataag ggagaaatat tcgaagccat gatgcaaaaa ggggtagaag
     7081 aggcaagaaa ccgagcaaaa ttctacgatt atattgaagt tatgccaaaa gcggtctatg
     7141 cgcctttgat cgagcaagaa ttggtaaaaa atgaacatga tttggaagaa attatccaaa
     7201 atttggtaga aatcggaaaa agtttggata aaatcgttgt tgcaacaggt aatgtacatt
     7261 atctgaacga agaagatgcc atttaccgaa aaattttgat caattctatg ggcggagcga
     7321 atccgttgaa tcgtcatagt ttgccggatg tccatttccg aacaaccgat gagatgctaa
     7381 ctgcatttca ctttttaggg gaagaaacag cgaaagagat cgtagtagaa aatacgaata
     7441 aaatcgcaga tatctgcgaa gaagtgattc cagtcaaaga tgaactttat acaccgaaga
     7501 ttccaggttc agaagatgaa atatcagaat tgagttacac aaaagcaaaa caaatgtacg
     7561 gagatccgtt acctgagatc attcaaaaac gattaaaaaa agagttgaat tctattaatg
     7621 gtaatggctt ctcggttatt tatctgatcg cccaaaaact agtgcataaa agtaatgaag
     7681 atggctatct agtaggttca cgtggctctg ttggctctag ctttgttgcg acgatgacag
     7741 gaatcactga agttaatccg ttagcccctc attattattg tccagagtgc caatattccg
     7801 agttttttga agatggtacg tatggttctg gatttgacat gccagaaaaa caatgtccaa
     7861 aatgtggtgc acgcttaaac aaagacggtc atgatattcc atttgagaca tttttaggat
     7921 tccatggaga caaagtgccc gatattgatt tgaacttctc aggtgattat caagcagaag
     7981 cccataacta tacgaaagta ttgtttggcg aagactatgt ttaccgtgcc ggaacgattg
     8041 gtacagtcgc cgacaaaaca gcatatggct atgtaaaagg atacgaaagg gacaataatc
     8101 tgcagtttcg aagtgcagaa gtcgacagat tagcaaaagg cgctactgga gtcaaacgga
     8161 caaccggcca gcatccaggg gggatcatcg ttattcccga ttatatggat gtctatgatt
     8221 ttacgccgat ccaatatcca gcagacgatc aaaattcgga atggaaaacg actcattttg
     8281 attttcactc gattcacgat aatgtattga aattagatat tctagggcat gatgacccaa
     8341 ctgttattcg gatgcttcaa gatttatcag ggatcgatcc acaaacgatc cctacggatg
     8401 acccggaagt gatgcggata tttgccggtc ctgaagtact tggagtaagt caggaacaaa
     8461 tttattccaa aacagggaca ttaggtattc cagaattcgg cactcgtttt gttcgaggga
     8521 tgctagaaga aacgcatcct acaacttttg cagaactact gcaaatttcc ggactatctc
     8581 atggtacaga cgtatggtta ggaaatgcgg aagaattgat tcgacgagga gatgcgacac
     8641 tggcggaagt aatcggctgt cgtgatgata ttatggttta tttgatccat gctggattag
     8701 atagcgggat ggcatttaaa atcatggaaa ctgttcgtaa aggacaatgg aacaagatcc
     8761 ctgatgaatt gcgagaaacc tatctatcgg cgatgaaaga aaataatgtg cccgactggt
     8821 atatcgattc ttgttcaaaa atcaaatata tgtttccgaa agcccatgct gcagcttacg
     8881 tattgatggc tcttcgtgtc gcttatttca aggtttattt ccctattttg tattactgtg
     8941 catacttttc tgtacgtgca gacgactttg atctagtctc catgtgcaaa ggaaaagatg
     9001 ctgtgaaaca agcaatgaaa gaaatcacgg acaaaggatt agatgcttcc gtaaaagaaa
     9061 agaatcaact gacagtgctg gagttagcca atgaaatgct ggaacgagga ttcaaatttg
     9121 gaatgattga tttgtataaa tcggatgccg ttaactttgt tattgaaggt gatacgttga
     9181 tcgcaccatt tagagctgtc ccaagtttgg gaaccaacgt ggcaaaacaa atcgttgaag
     9241 caagaaaaga cggtccattt ttatccaaag aagatcttgc cacccgcggt aaagtttcaa
     9301 aaacattgat cgaatatatg aacgacaatg gcgtattgaa agatctacca gatgaaaatc
     9361 aattatcgtt attcgatatg ctataagcat ctgttttcct gtctgcttgt aattttaagc
     9421 agagtatggt atagttctta taggtaatga tactaactgt gagtgagcgg atttttcgct
     9481 cactcttttt aatggaatta cgcacttaat tttttaagga ggcgaaactt ttgagtagcg
     9541 tcgttgaaac agtcacagaa atggtgacac cgattttaga tgaacaaaat tttgaacttg
     9601 tcgaagtcga atttgtaaaa gaaggaaaaa gctggttctt acgagtattt attgacaaag
     9661 aaggcggtat cgatatcgaa gaatgcgcct ttgtcagtga gaaactgagt gaaaaacttg
     9721 atgcagcaga tccagatccg attcctcaag cttatttcct ggaggtatcc tccccgggag
     9781 cagagcggcc attgaaaaaa gaaagcgact atgaaaaggc acttggtgat tatgtccatg
     9841 tctccttata ccaaccagta gatggcgaaa aacaatatga aggatttctt caatcatttg
     9901 atgctgaaca gcttacattg aaaatacgca taaaaacacg agaaaaagaa ttgacttttg
     9961 accgaaaaaa tattgcaaaa gctcgcttag ctatccaatt ttaatcacgg agaggaatca
    10021 gaaaaaatga gtaaagaaat gttaaatgcg ttagacgctt tagaagctga aaaagggatc
    10081 tcaaaagaaa tcgtgatcga tgctttagaa gcagcattgg tttctgcata caaacgtcac
    10141 tatggccaag cacaaaacgt cgaagtggaa tttgatcaga aaaaaggaaa gatccatgtc
    10201 tatgcggtaa aagaagtaac ggaagaagtg atggattcac agttggaagt ctcattgaaa
    10261 gatgcacttt taatcaatcc agcatatgag atcggtgata cgatccgttt tgaagtaaca
    10321 ccaaaagatt ttgggcggat cgctgcgcaa acggctaaac aagttatttt acagcgtgta
    10381 cgtgaagcag aaagaacgat tatttataac gaattcagtg cttatgaaaa agacatcatg
    10441 caaggaatcg tcgaacgcca ggataaacgt tatatttatg tcaatcttgg taaaattgag
    10501 gcagttcttt ctaaacaaga tcaaatgcca aatgaatttt atcaaccaca tgatcgaatc
    10561 aaagtttatg tttcccgagt agaaaatacg tctaaaggtc cgcaagtatt tgttagccgc
    10621 agccatccag acctattgcg ccgcttattt gagcaagaag taccagaagt ctatgatgga
    10681 atcgttgaaa ttgtcagcat cgcccgtgaa gctggcgatc gttcaaaagt ggcagttcgc
    10741 tcaaatgatc caaatatcga tgcagtcggt acatgtgtcg ggccaaaggg acaacgagtt
    10801 caagcaatcg tcaatgaatt aaaaggcgaa aacatggata tcgtcgaatg gaatgaagat
    10861 ccagctgtat tcattgcaaa tgcattgaat cctgcacaag tcatggatgt gatcttcgat
    10921 gagacaaatc caaaggcctg cacagttgtc gttccagact accaattatc gctagcaatc
    10981 ggtaaacgcg gacaaaacgc acgtttagct gctaaattga ctaaccataa aatcgatatc
    11041 aaatcagaat ctgacatgac ggaattttat gaaaatcaag aacaacaaaa agaaacagaa
    11101 gaactgcatg atgaagcaat cgttcagtca gatttaacag acgatgaata tgaaacaatt
    11161 gcattcaaca atgaaacggt cgaagaaaaa ccagaagctt aactttagta aggaggcaaa
    11221 gagatgaaac aaaggaaaat tcctttacgc aaatccgtgg tttcaggtga aatgaaacct
    11281 aaaaaagaga tggtccgaat cacccgttca aaagaagggg aagtatcgat cgatccaact
    11341 ggaaaaatgc ctggacgagg agcgtatgtc tcacttgagc cggaagaagt acaacaagct
    11401 tgggataaaa aaattctcga tcgagtactc gaaacaacat taacagatga gttttatcaa
    11461 gaacttcttg actatgtcac tcaccaaaaa gcacgtaaag agttatttgg aaaatgaata
    11521 cagttaatcg gcaaaaagcc atgaatctca tcggtctagc gatgcgcgcg ggaaaactaa
    11581 tcacgggtga agaactgacc attgctgaaa tccgcaaaca aaaagcaaaa ttggtgtttg
    11641 tggcaaccga tgcaagcgag aatacaagaa aaaagataaa agataagagt tcatattatg
    11701 aagttccttg ctttgagcta ttttctgaag cagaaatcac gcagatgatc ggaaaacctc
    11761 gcaaggtatt cggaataatg gatgccggat ttgcgaagaa gaccaaggag ctaattgaag
    11821 gttaggaagg tgattgcatg ggcaacaaaa gaatctatga attagcaaaa gaactcaaaa
    11881 aatctagtaa agacgtcgtg gaaaaagcac aacaacttgg catcgatgtg aaaaaccata
    11941 tgggtgcgat tactagcaat gatgagaaga aattgcaaca agcgttcaaa aacccaagca
    12001 cgaaacaaca agctgcacaa caagcaagca gaaaaccagc caatcaacag ccgaacgatc
    12061 aacgtaaaaa agaaacaacg aacaatcaac aaaaaaataa aaataaccgc agttatcaag
    12121 atcgcggtca agggagcggt caagtgaatc aaggaaaaaa accaacaaca aaccaaaata
    12181 atacgggaaa taccagcagc caaaatcgtc aaggaacgag ccaaggcggc aatcaaaacc
    12241 gtactaacaa taacaacaaa ccaaacaata acaatcgcgg caaatttaat aataataacc
    12301 gtaatcgtta taataaaaaa ggtaaaaaag gcaaacagca acaatcaact aaaccagctg
    12361 taccaccacg caaattccgt gaattgccag atgtattaga gtatactgaa ggtatgaacg
    12421 tagcagatat tgcgaaaaaa attcatcgtg agccagcaga aatcattaaa aaattattta
    12481 tgcttggtgt catggtcaat caaaaccaag cactggataa agatacgatt gaactgttag
    12541 cgacggacta tggcatggaa ccacaagaaa aagtccaagt tgacattgct gatatcgata
    12601 aattcttcga ggcagaagaa gtcgatccag ataaattagt ctcacgtccg ccagtcgtaa
    12661 cgattatggg acacgtagac catgggaaaa caactttatt ggatacatta cgccattcac
    12721 gtgtaacaag cggcgaagcc ggcggaatca cacagcatat cggtgcctac cagatcgata
    12781 ttgatggtaa accaatcact ttcttggata caccaggaca tgcggccttt acaagtatgc
    12841 gtgcacgtgg agcaagtatc acagatatca cgatcttagt tgttgctgca gatgatggcg
    12901 tcatgccaca aacagttgaa gcaatcaacc atgccaaagc tgcaggcgtt ccaatcatcg
    12961 tcgctgtaaa taaaattgat aaaccaggtg caaaccctca acatgttatg caagaattaa
    13021 gtgagtatga gttaattcct gaagcatggg gtggagaaac gatctttgtt gagatctcag
    13081 cgaaattcgg ccaaaacatc gaagaattgc tggaaatgat cctgttagtc gcagaagtag
    13141 aagatttaaa agcagatcca acacaacgcg caatcggaac agtgatcgaa gcacgtcttg
    13201 ataaaggtaa aggtcctgtg tcaaccttac ttgtccaaga aggaacactt catgttggtg
    13261 acccaatcgt tgtcggtaat acttacggac gtgtccgtgt aatgacaaac gatcttggcc
    13321 gtcgcgataa agaagcagga cctgcaacgc cagtagaaat cactggcttg aatgatgtac
    13381 ctcaagcagg tgaccgtttc gtagtcttcg aagacgaaaa gacagcacgt gcagcgggtg
    13441 aagaacgcgc gaaacgtgca ttattagaaa accgtgctgc cacaagccgt gtgactttgg
    13501 ataacttatt tgaaagcctg aaagaaggcg aattgaaaga agttaacgta atcatcaaag
    13561 cagatgtaca aggttcagca gaagcactgg ctgcatcatt gaagaagatt gatgtggaag
    13621 gtgtccgtgt aaaaatcgtc cattcagctg tcggagcaat caatgaaagt gatgtaacct
    13681 tagcagcagc cagcaacgct attatcatcg gtttcaacgt tcgtcctaca ccgcaggcaa
    13741 aagtgcaagc tgatactgaa gaagtcgata ttcgtcttca ccgtatcatc tataaagcca
    13801 tcgaagaaat cgaaactgcg atgaaaggga tgctagaccc tgaatttgaa gaaaaaatca
    13861 ccggtcaaat gatcgttcgt gaaaccttca aagtttctaa agttggtacg attgcaggtg
    13921 cctatgtaac agaaggatat attcgtagag atagcggtgt ccgtgtaatc cgtgacggaa
    13981 tcgtaatcta tgaaggccaa cttgcaagtc tgaaacgatt caaagatgac gtgaaagaag
    14041 tcaaaatggg ttatgaatgt ggtgcaatga ttgaaaaatt caatgacatc aaagtagatg
    14101 acgtaattga aggatttatc atggaagaag tcaaaaatga ctaaaaactg ataaaaggag
    14161 gccaatcatt atggctaact accgtgaccg ccgagtcggt caagaaatct taaaagaagt
    14221 caatgatatt ttgcgcaaaa aggtccgtga ccctcgtgtg gagaacgtaa cgatcacaga
    14281 tgtgaaagtt acgggggact tacagcaagc aacgatctat tacagcattt tatctgactt
    14341 agcatcagat aagaaaacag cacaagatgg attgaataaa gcatcaggat tgattcgtcg
    14401 agagttaggg cacaatctaa gtatttataa gacacctgaa ttgttctttg aacttgatga
    14461 atcagttgtc tatggcaacc atatcgatga attgcttcgc gacttgaaca aagactaatc
    14521 aaaaactccc gcaaatcctg tgaaatcagg ctctttgtca ataaggactg atgagttgtg
    14581 caaaatcaaa tctgagtcag aatgaaccac attctggctc ag
//
LOCUS       DAZKLA010000063        14582 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000063 DAZKLA010000000
VERSION     DAZKLA010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.064"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..582)
                     /locus_tag="ACG85N_001688"
     CDS             complement(<1..582)
                     /locus_tag="ACG85N_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0224456.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKY"
     gene            complement(830..1666)
                     /locus_tag="ACG85N_001689"
     CDS             complement(830..1666)
                     /locus_tag="ACG85N_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFQ0224457.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1677..2606)
                     /locus_tag="ACG85N_001690"
     CDS             complement(1677..2606)
                     /locus_tag="ACG85N_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFQ0224458.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2798..3151
                     /locus_tag="ACG85N_001691"
     CDS             2798..3151
                     /locus_tag="ACG85N_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0224459.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(3225..3881)
                     /locus_tag="ACG85N_001692"
     CDS             complement(3225..3881)
                     /locus_tag="ACG85N_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0224460.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            4107..6041
                     /locus_tag="ACG85N_001693"
     CDS             4107..6041
                     /locus_tag="ACG85N_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0224461.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(6127..7350)
                     /locus_tag="ACG85N_001694"
     CDS             complement(6127..7350)
                     /locus_tag="ACG85N_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0224462.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7344..9215)
                     /locus_tag="ACG85N_001695"
     CDS             complement(7344..9215)
                     /locus_tag="ACG85N_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFQ0224463.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(9236..10354)
                     /locus_tag="ACG85N_001696"
     CDS             complement(9236..10354)
                     /locus_tag="ACG85N_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0224464.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10437..11270)
                     /locus_tag="ACG85N_001697"
     CDS             complement(10437..11270)
                     /locus_tag="ACG85N_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0224465.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11263..12180)
                     /locus_tag="ACG85N_001698"
     CDS             complement(11263..12180)
                     /locus_tag="ACG85N_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0224466.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12407..13531)
                     /locus_tag="ACG85N_001699"
     CDS             complement(12407..13531)
                     /locus_tag="ACG85N_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0224467.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            13714..>14582
                     /locus_tag="ACG85N_001700"
     CDS             13714..>14582
                     /locus_tag="ACG85N_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0224468.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYT"
BASE COUNT         4606 a         2883 c         2349 g         4744 t
ORIGIN      
        1 atatttttta attgtgccgt ttacgagaat ctcttcatct ttcaatacta ggcctatttt
       61 atttttataa cctggcatgc tcattagata aaggatttca tcgtaatgga tatagtgatc
      121 gctattacca taacggacag ttgtccacct ttttttcgta gcatgttgaa caggatagtt
      181 ttctttttct ttcaataagt aactttggat gcttttttta tttttttcat aagtgaattc
      241 ttttatcaaa taaccactag gcgtaatctt tcgattaata acagttattg ctttattcgg
      301 atgagacgtt agataaacaa ttttacactt ttcattcttt tctctaagca tttcgcctag
      361 ttcgataccg ctataatagg cgttcaaatc gatgtctagg aaaaaaatat ctgaggaata
      421 gatttcatta tttttaatga attcaacaaa ttgtataacg ttcaatccct gaacgatctc
      481 aaatttccaa ggacttaata aaattctgga ttcttccaaa atttctacta gcattttttc
      541 aaaaaccaaa ctatcatcca aaataaatat acgcgttacc ataaaaagta cctccactcg
      601 atattttact atcaaaatca tttcatacaa cttttcaaca tacatgttag gaataaattg
      661 taattctctt cacaatcttt tccaacacct ttgaaaccga tatcaaatac tttcaggatg
      721 ctgcttctaa aataaatatc aaacaaatga atacatcatg caaaaaaagc cagacaaccg
      781 acgtccttgc gtgtcagctg tctgagctat gtttatttat tttgttcttt taaaaagaac
      841 gatacacata ctgatttttc ggagcagaga ctttctcagc gtttgtaaca actacagatg
      901 ggatttcacg cttgaatggt tgataatagt ctgattggat ggttccttct attttatacc
      961 attcatcatt tttaaccgtt actccttccg gcatatgcgt caacaaacca aagactcctg
     1021 aatctgccac acaatggata atgccgaatc ggaacaagaa gatatctgta ttgctatcgt
     1081 tcggtgtttg gtaaacaaat ccttcatagc tgattttttt gccaatgaat tcgctagggt
     1141 aattataaat tagctccata acttctaaat agttttcatc tgtgatcttg atagtttgct
     1201 tatccccata cttttctttc aactcattca tttggtctaa gtagtctgat ttgttgaaat
     1261 aaatactggt atccggcttt aagtattgtg tctgcatctc cggatcccct actgattctt
     1321 tacttactgg gaaattaaat cctttggctt ctacgatcgt agtgtcgaga ctaactgttg
     1381 gaaacaacgt ccccacgatt actggcaaag ctagcagtac ataagcgatt ccacgttgat
     1441 aaggttttga caacccatgg tcatgatcgt gctcgtgtgc cttttcttct gcttttccgt
     1501 ttttgaccca caaatacagc tgaactaaag ccaaaagagc agataaaatc attgaaagaa
     1561 tagctaagta gcgataatgg acattgatat attgatcgag tttgccagaa acttgcaagt
     1621 acatcatcaa ttcgaaatac ccagataaaa tcaaaaaccg cagcatattt tccctcctaa
     1681 ataaataatg tgaatccaat aacgctaagg gcaatcagtc cgatcagcga taaaataaag
     1741 cgtcctgtaa aatatcgttt catcatcagt agatttttga tatcgaccat tgggccaaag
     1801 accaaaaagg cagctacagg tgctgtgccg aacagactta acaaagaaga tcctataaac
     1861 gcatctgctt ctgagcaaag agacatcgta aaggccaata acagcatgat gataatggct
     1921 aaaatctttg ttgagccgag cgttagcata attctagttg gtaaataagt ctgcatcgca
     1981 gctgcgatta atccgccaat gatcaaataa cgacctgtat caaaaaattc gtcgattgaa
     2041 tgtgtcaaca catgcccagt ttgatggatc agctgatttt ttggttcatt cttctcactt
     2101 tgttcttctt catggacatg atgatggtga ttttctgcta ctttagacgt gattttttct
     2161 ttcaatacag cgtctttttg aaaataggca agccagacac caatcacaag ggcaacaaaa
     2221 aagctgccta tcacccgcca gcttgccatc tgccaagaat tgccaaatgc gataaaagtg
     2281 gaaaaaatca cgattggatt gatgatcgga gcagtcagca taaaagcaaa cgctgtgtga
     2341 actggtacgc cttttttaac aaattgatga acgatcggta cgatgccgca ttcacatgaa
     2401 ggaaagaaaa atcctaagat cgaaccagta aaaatagaga agatacgatt ttcaggaagc
     2461 catctcttca cacgttctgg ggtcaaaaat acttgtaagg cgcctgagat aagacagcct
     2521 aataatacga aaggtaatgc ttcaataacg atcgataaga aaatcgtact catctgcaaa
     2581 accgaatttg gaataaaaga aaacatgagt ttcctccttt ttatttctag ttcattcgct
     2641 ttgcaaatat acgcttttat tcaaaaaaaa caagttgctc tgtaaatttt tacacaactc
     2701 ttatttttta ccaaagcact tcaattgact ttttctgtta ttcccattaa attaaggtct
     2761 catacatatc acttctatca taaaagggag aataaaaatg aaacaaacca ctaacttgac
     2821 tgaagttcaa gacatactgc aacagtcatc cgttgccgtc atttatcttt cgatgcctaa
     2881 ttgcagtgtt tgtcacgctg ttcgtccacg actagaggaa cttctcacac attatgatat
     2941 tccggcactt catttggatg catttgaaac accagaagta gctagtaaat ttgaagtgct
     3001 gactgcgcca gttgtcctga tttttcatca aggaaaagaa gtctttcggc aagcacggtt
     3061 catcgacttt aaaaaaatcc gttatctact agatgaactg acagcagaag atgattccct
     3121 ttcttatgaa gaaatcttcc gaacaaaata atcgttgtcc aaacagaaaa aagcgattga
     3181 acaagtgaaa gttttgcctt catttgttca atcgcttttt atttttattc tgctgtattt
     3241 tcggaatgcg catttgcgtc agaatgcaag aaatagatca aggtttgcaa ttcattcgtc
     3301 aaatcaacat tttggatcaa tacatctgac ggagcattca atcgagcagc ggtaaagttc
     3361 aagattcctt tgattcctgc ttccgttaac tgattgacta cttcttgtgc tctttcagcg
     3421 ggtatcgtta aaattgctac ttcgatttgt tggatcttga tttgttctga catatcttcc
     3481 attggataaa cggggatacc gtctacgatg cgcccaacga tttcctcatt cacatcaaaa
     3541 gcacaactga tacgtatgct attactttga tggaatttgt attttagtaa agcacttccc
     3601 aagttcccta caccgatcag cgcaacattt gtcaattgat cgtcgtttaa tgtctttgca
     3661 aaaaagtgca taagatcttc tacatcataa ccatacccac gttttcctaa ttcgccaaaa
     3721 taagaaaaat ctcgacggat cgttgcacta tctacttgaa cggcttcact taattcagtt
     3781 gaagacactt ttttctttcc agcattattt aaaatcctta aatagcgata atacaaaggc
     3841 agtcttttag ctgtcgcctt tggaataata tgatctttca cgaaatgacc tccacgtatt
     3901 gtctaattac atctttgatt tctccatcat agcagtattt ataggaaaaa atcaattcca
     3961 ttgctcagat attgtgaaat tttattcttt attcatttat ttatcatttc tgcaaatctc
     4021 gcagtataat ggaatttatg gtagactact aattgaaaaa tagtttttta tttgaggaga
     4081 attcacaaag tagaaaagag gattccatga tattattaca agccaatcag attgctcgcc
     4141 attttggtgc agaagtctta tttgaaaaca tacatttaga aatcagtaca ggcgctcgtg
     4201 tcgctttagt tggcagaaat ggcgctggaa aatcgacttt attaaagatc attgctggaa
     4261 ttgaagcacc tgatgaagga aagatcgcta aaaataaaac agcgactctc ggatacctcg
     4321 ctcaggacac aggacttgat tccaatgaaa cagtttggaa cgagatgctg aaagcttttg
     4381 aacaagttag acaaatggaa gagcggatgc gcgagctaga gattttcatt agcgaaggta
     4441 cgccagacac acctgcttat agttcttatt taaaagaata cgaccggctg caacacgatt
     4501 tttcagatcg aaacggctat ggttatgaaa atgaaattcg ttccgtattg catgggttta
     4561 aattcgattc ttcattttat gatcagaaaa tcgatacttt atcaggcgga cagcgcacac
     4621 gtttagcttt agctcgcatg cttttgcaac accctgacat tttgattcta gacgaaccaa
     4681 cgaaccattt agatatcgag acactttcct ggcttgagaa ttatctgcaa agctatactg
     4741 gtgcattatt gattgtttcc catgaccgtt attttttaga taaagtagtc aatgaagtct
     4801 atgaattaag cagaagaaaa atgcgccatt acaaaggtaa ctattcgaaa tatctagatt
     4861 tgaaagcaga acagttagca agtgaatgga aggcttatga aaagcagcaa acagaaatcc
     4921 ataaattaga agacttcgtt gctcggaacc ttgttcgcgc ctctaccaca aaacgagcgc
     4981 aaagccgcag aaagacatta gaaaaaatgg accggctgga tcgtcctcaa ggggatgaaa
     5041 aacaagcaaa ctttctattc agtatagaaa agccttctgg aaatgtagtc ttgcaaatcg
     5101 aagatgccgc tatcggatat gataatgtca ccttatctga accaatcaat ctagatatcc
     5161 gtcgtcaaga agcgattgct ctagtaggtc cgaatggtat cgggaaatcg acgttgctga
     5221 aatcaatcat tcaccagatc ccttttatcc gtggaaaaga aacgctaggg acaaatgtca
     5281 ccatcggcta ttacgatcaa ggtcaagctg atctgcatac aaacaagacg atcctagcag
     5341 aattgtggga tgagcatcct accactccag aaaaagagat ccgaagtgct ttaggtgctt
     5401 tcttattttc tggcgaagat gtagaaaaac caatccctct gctaagtggc ggtgaaaaag
     5461 cacgggttgc tttagccaag ttagcgatga acaaagaaaa ttttttgatt ttggatgaac
     5521 caacaaacca tttagatatt gacaacaaag aagtattaga aaatgcattg atcgattatg
     5581 acggcacact gctttttgtt tcccatgacc gatatttcat caatcggatc gcgacaaaag
     5641 tcgtcgaact ctctgaaaat ggcagcaaag tctatctagg agactatgat tattatttag
     5701 agaaaaagca ggaagaagaa gaaatcgccg aattgttggc acaagatgag ccagaatctg
     5761 ctcctgctcc cacgaacaaa tctgattatt accaaagcaa agaacagcaa aagcttatcc
     5821 gtagccttca acgaaaaatt actcaggttg aagaagaaat ggcgcggatc gatgaatgga
     5881 tcgatcagtt ggaacaagaa atggtcgcgc cagaaaacct ggaagatcac gtaaaactca
     5941 atgagctgaa ccaagattta gaagctgcta gacaagatca agaaacaaaa ttgacggaat
     6001 gggaagaact aagcttggaa ttagaagaat atgaaaatta acgaaaaaac caaaacagaa
     6061 accattcatg ctgttttggt ttttcttttt ttattttatc aaacaaaaat tccatattta
     6121 cctttttcac caataagaag accaattact agtcattctc actaaagctt ctaaataata
     6181 gtaatcaccc caaatacaag gtgtatttac cccattattt ttgggcttat tataaacaga
     6241 ctgtgttaaa aagccatctt cttcttccgt tgtagcatat ctttctttca attgtcgcat
     6301 aattttccaa acagcagact ggtataagtt cttttctgtt ttgtctaata gacctgattc
     6361 tattaattct aataatccac aaaccatgat caccatacta gatgtatctt tttcttctct
     6421 gctagaatca tcaaagatca aatcccaata acaaatggta tcttctggca accgattcat
     6481 aaaataattg gctaatattt tgcttaaatc aaaatatttt tgttctttca aataacgata
     6541 acttaatgca aaaccgtaaa tcccccatgc ttggcctcta gcccaacagg aattatcact
     6601 ataaccttga tgcgtacttc catatcttgg ttttccatct ttgacatcca tataaaatgt
     6661 atgataagtt gaaccatctt ctcttactaa ataatctgct gcattttgaa tatgagcctg
     6721 agcagctttt ttatattttt gatttccagt ttcttcactc gcccaataca ataatggaag
     6781 attcatgcaa caatccatga tcattctacc ttgttgtgaa ggatcactag gatctcccca
     6841 agcttgtata actcctgcct tctcccaaaa acgctgaagt aatgtttctg cagcatcaat
     6901 tgaagcttcc ttcatttttc gatttcccgt tattttataa tctgctacgg ttgataaaga
     6961 atacaaaaaa ccaagatcgt gatgattagt gataatttga cagtcaatcc tgtggataaa
     7021 atctatatct gctttttcag ctgtttgttg aaataaagga tcacccgtct tctcaaataa
     7081 caaccaatta attcctgtcc aaaatccttc tgtccaacca atattgtctc ttaatggata
     7141 acgtcctttt ataggacact cgcttgggaa ttgatactga ctgttactga tacgtttgat
     7201 taacctctcc gtgtgtctgc gacaacgctc gatctcatta ttccattctt tttccattaa
     7261 ttctttataa cattcattta tccatttttc tggatgtatt atctcttcaa acatcacttt
     7321 atcaagcatt tccccaccag cctttagcat aattttagtc tttttttatt gtttttattt
     7381 tggatagtta gcccggttgt cccatcaaaa tcaattttgg gacgattgtt ccatacttct
     7441 aaactataat cccgatcttg atgagcaacc actcctgttc cgagccaatg ggttcctttt
     7501 ccaaatgtac cctctaatcc aggaataaaa gtgttttccc atgtcatcaa gtttgtattg
     7561 ggcaaaccca taatagctgt ggattttcgt ttaatagtat cgtcacttaa acagataata
     7621 cctgaaaagc cagcttcact caccctatag ttttccttgt cttcttctat gattattcct
     7681 tctttctcag gagaacattc aatagcaaac cctgtctcat atagaacata ttctgtatct
     7741 aattctactt tgtgtacgcg aatctgccag ccttcaaaag gaataagcca agttctgatt
     7801 tttgcctgta aagtaggttc ccatgtagag gcaagtacct ctttcttaca tacaatgtct
     7861 cgtacattat ttcgaacgat gaactgtcca taggttttga tgctctcatt cttaaaactg
     7921 gtcgtgatat atgtgtccgc tcgctgaatc cctagcattg aatccattgc catttctgct
     7981 ttcaattgat tcgctcgcgg tatcgaaaaa ccaaattgac tagaataagc aaattttaaa
     8041 tatttttcat tggtcaatgt atggaaattt ggccctaatt gtcccgcatt caaaaaaaat
     8101 gtatgtccat tatcatgttg tatgagcaaa ttggcttctg aaagaagttt agttccttca
     8161 cgaataggca taggcatttc tttagccgtc caatactcat ggtcttcagg tagcgccaac
     8221 aataaaaaaa tcttattcag ccataatgga gataacgtac tattataggg ttcagtcaat
     8281 gtcaattgag ggtaggtata accaatattc aggatacctt tttcatcaaa tatttctttt
     8341 tgcatccacc atcttatatt ccgattgact aaccccttga tttcacctaa tttaaaaggc
     8401 aaaagatcgg cccacaacat agctgaccaa aagcttgaaa ctgcaaaacg ataaatcatg
     8461 cttcgaccaa aaggcacatt tgcaccatct tccgcaaaaa aatgaatata gtctctagaa
     8521 aaatcaatag ctcggttctt atatatctgg ctaatgggct tttctggttc tagtacgcta
     8581 taaatcaaac cataataatg aaatgcaaaa ggattataat aatcttggcg acctcttgaa
     8641 ctgtctcgat accagccatt ttgaagataa caattttcta tattatcaaa tgcttctttc
     8701 agtgctgatt ctttaactgg ataaccaaat ttttggataa ttgttccgac aattatctta
     8761 aaaaattgcc aattaccatc tgcacattca tattcaaaaa tttgcgagaa ccaatgaata
     8821 atctggcttt tttctcctgt atctaatttt ttccataata attgttcatg atgatataac
     8881 gctaataaca ttgcgggcat ttctaccata cgctggtctc tttcatgtat ttctccccaa
     8941 taatcgggat gggaaggatc tgttccagct tttattcctg atagaattaa tttcaaccat
     9001 cgttcatcta attggtcaat tgcgggcccc gctccccata aaacccgaga gaatccttcc
     9061 attcctgcaa ttttatttcc atatcctacc ccttgatgta tataatcaat ttgtgcgcca
     9121 cttgctcgat aaaacggctg gagtggttga tttatctttt taaataagtc aaagtaatcc
     9181 tgtctggtcc ttaatagtgg tactggatgt ttcattgtat tttctcctct ttttactacc
     9241 aaatagctaa ttccgactgt tctaatcgaa gtaagccttc aataaaaaaa taatcagaat
     9301 agatcagtgg cacattataa gtcagttctc tatggtatgc ttctgctccg cctccaatga
     9361 tcccatcttc atccatattc caatttgcat agtgcttttc tgtcttttgg agtatattga
     9421 tagcagcttc atgatacatt tttgatcgta cagaggaaac atatcctgct aattccagaa
     9481 gaccgcatgc agcgcatact ccagcactgg tatcaatatt cgaatctttt acagcaaaat
     9541 caatgagagg aatataacca gttttttcga ttttctgaat aaaatttact gctatctttt
     9601 ctgctatagc tagatagtac ggattttttg tgtaacgata acttaacgcg aatccatata
     9661 aagcccatgc atttcctctg ctccaacaag aattctcatt gtatccttgc ccgccaagta
     9721 accgaatata tttccctgat tctgcatcaa attctgcgat atgcccggtt gatccatctt
     9781 tcctcactag atacttagct gttgtatctg catgctgccg agcaatttta gagaacctcg
     9841 gatcaccagt ttccctcgaa gcccaataca gtaatggtaa attcatcata ctatcaataa
     9901 tcatccaacc aggtttatgg tcattccatg ctactaaata gttccctgaa atattatagc
     9961 gtccagcaag taccgtagct gcatgtaacc ctagttgtcg actatgtttg ttttgagact
    10021 gttgataatg tgcaatagcc gatggtaaga ataaaaaacc tacatcatga tgaacgtctg
    10081 aaaaatggta cagggcagat tctaataatt gttcttgttg tattgctgcc tctttgaact
    10141 tttctcgttt tgtcttgtga aagagctgcc ataaaatacc actccaaaaa ccattggtcc
    10201 accagtcgat tcctctgtcc tttattacgt ctgtatattt tccgttgtaa gaaatatatg
    10261 gaattttatt ccccattctg tctaattccg catctatctt ttcagaagct acattcaaaa
    10321 tttttcttga ccattccttc gtctgttcat tcatggatat cccgccattc ttttaatttt
    10381 tatgaatgtg gagttgctcc tagaacgaac tagatggtta acattcgtta ttttttttac
    10441 ttatttgcag ctccaatgtc attctcctta aatttagact gcgcccacac ataaatcaag
    10501 accattggga tcactgaagt tgctaaaatt agcataattg cgccataatt aattggctca
    10561 cctgtactag aaaccgtcat atttttgata attaagggaa ccgtatattt atctttatct
    10621 gaaagcatga ctaatggaaa tagatattga ttccaacttc ccataaagat aagtaaacca
    10681 ctagtaataa tcgtaggtga taaaatcggt acagcgattt tcgtaaaaat cacccactca
    10741 ttggcaccat cgattctggc agcctccatc agtgctgtag gaaattgact gctatattgt
    10801 cgcatcataa acactgaagt tgtactggca atcgaaggga tgatcacacc tgcataagta
    10861 ttcgataatc ctaaacgact cataatgtaa aataaaggaa tcaatgtggt aaaaaaaggc
    10921 accatcctag atagcataac tgtattgaac aacagtttat tccccataaa acgatacttt
    10981 gctaatgcat aacccgccat tgtagaactg gctgtagctg aaactgtact gattacagca
    11041 ataacaatcg aattccaaat aatcaaggga taattatatc tcgccatgat ggcttgccag
    11101 ttttttaata gcatatcccc aaacttgaaa ttgattccgc cacttaaaat ttcttgatta
    11161 ctttgcgttg aagctacaat catatataaa tatggcatca aaaatattaa ggcaccaaac
    11221 acaataatga cgtacgtaaa aattctgctt tgtatctttt gtttagtcat ttttatcacc
    11281 tccgaattta aattgaatag ccgataatat aacagataca aacaccatcg tccaggcaat
    11341 agcagaggct tttccaaaat caaccatttc aaaacttgtt ttatataaat tgactcctac
    11401 gaccaaagct gaattttctg gtccattgga agtcccaaag aatatatttg ggatattgaa
    11461 attacccaag ccattgatag tggctaaaac tgtggccatt aaaatgattg gctttagcat
    11521 ggggatcgtt aataaaaaga attgccgaaa tttcgatgct ccatctattt cagcactttc
    11581 gtatatctct ttagaaatcc cttgaagacc agctaaaaat aaaatagtgt tatatcctac
    11641 actaagccaa atcatcgtga tgatcattga tattcgtatc caaaaaggat ttgttaacca
    11701 ctgtacaccg ttaattccta gcgcattcaa aaatccattg aataacccag tcggattgaa
    11761 aaaagtcatg aatacggaag ctacagccac cgttgatgta atggttggaa tataatagac
    11821 agttctaaaa aatccttttc cttttaactg tgctgaattc aataaaacag caaagtaaat
    11881 agctaatagc atcgttattg gaattgatcc tgccatgata atcaaaacat tcgtgtaaga
    11941 tttaataaac aatggatctc ggaagatagc tagataattg tcaaatccaa caaattcaaa
    12001 tgagtagcca attttcataa aactaaaagc aaacgagata atgatgggaa tcaaaatcgt
    12061 cacaataaag aaaataacaa aaggtgtgac caatccataa gcactttttt tatgagacgt
    12121 atctgttgct tttttcttgc tgttctttat tgtcttgctt ggtaacgaag attgcatcat
    12181 cgttaagtcc tcctaatatt ttttaatcac cagttaattt tacgccgtac ttatgagcaa
    12241 aattttctgc ttcttttaga tatgaatttt caaaatcacc tgtttgccaa tagttatatg
    12301 cagcggtagt caaataagac gtagcatctg aataagtggc tggtaaaaca gtatttgatg
    12361 tattttcatt gattttattc aatagttcat agtatttttg tcctccttag tagtcaaact
    12421 ttttctcagc tgcttctgtt tgataggcca atgagttaga tgtagccacg cccagctcca
    12481 gtgcgttggc taagcattct tcatctgtca gcataaatgt aagaaattgt tgtgccactt
    12541 gtggattttt ggcatcttta gggacataaa aacttgatcc tcctgatact gcaccttttc
    12601 ctggattttc ttttgaaaaa ggaatcagtg gagccatgcg ccatttgtca gatgcatcag
    12661 gaaaattcaa agacatattc gtagcatacc agccgccagc aacaaacatt gcagattctt
    12721 gaaatgcttt ctcagcatct tgtgcaccgt agaaatgtac aatatctgag tcgatcattt
    12781 ctttgattaa ctttgcagca tgtttcgctt ctttagtatt cagacggata tttccttctt
    12841 catcaagcaa agattgattc tgctgggcta gcatgttgat aaacaattct tggtccccag
    12901 attctggcaa ggcaattcca aaactaccag ttttttcttt taatgctttt gccacctgga
    12961 tatattcttc ccaagaactg atttcttcaa aatctacacc tatttcagca aatttttttg
    13021 gttgataata acaaagtgct ggggtaaaat cactagccca aggaataatc gtttcattac
    13081 tcgtttgttg acgcaaaata ttcaattttt gatcataaaa ctgatcgcca tatttgtcat
    13141 aaaatccata ttcatcagca ctataaaaag catcttgaaa tttttccagc acacctgctg
    13201 ctgaagtgtc tgccatggag ataatatccg gcatatcttc gtgtgttacc aattttgggg
    13261 tgatcatttg tactgttgct gttcccgaag ctacattcac aatttttaag tcaatatttg
    13321 ctccagtttt gcggttatag atttttgcag cattttcaac atagtttgtc agtttgccgt
    13381 ttgcgacata tacttctaat gtagtggctt tttttatcat tggatctgcg atgtttgctg
    13441 ttacttcttc gttagttttt ccacatgcac ttaagctgaa taatagggta atgtttagca
    13501 aagctatcac caatttgtat ctttttttca tgtctatccc ttctttcttt tcaagttctt
    13561 atataaaacg ctttcactta tgtatattaa cggaattagt aacacaaata tttatataaa
    13621 aaaatatttt acttgtcgtt ttggcttttc atttttgtta taatagtcat taattataaa
    13681 tagaaagctt tttatataaa aagagaaagg aaagtggttc ttttgttaac aaacaaccaa
    13741 tcctccaatc ttgtcaaaaa aacactaacc tattccatag atacccatct tcatttatct
    13801 gctgctccct attttcgact actaataaat gaaagcgcta atttatatgc tgaatttgca
    13861 cataaatcta tagaactaga taatccttcc cttttattca ttgataaaca gcaagaagtc
    13921 caaatctttt ctactcaaaa ggaaacacaa acttttatca ttatccagtt ttattttgat
    13981 gagcaattat tagcacatat tacgggacaa tctttttcta tagatcaaga aataacatct
    14041 atcattactt ttctgaaaaa tgaacagttc aaaattgatt atctaaaaca tagtaagcca
    14101 gctatttcct atgatcagga aatcaattct tttttcatac tatgccaaca gatgagcatt
    14161 catttgcttt ccttattagt tacttattgt ctgcaaaaaa ttcaattgtc ttccttacca
    14221 aggcattatc aacaggaact tgctttattg acaatacctt caaatgagaa agtcattcct
    14281 aaaaaaaagc atattcttta ttcagatgaa ttagttgatc caatcatacg atatttagaa
    14341 gaacatcttt cagaaaattt tttgcttgaa caattagccc aaattttttt tatcagtcca
    14401 gccagcttaa aaaaacattt tagaaaagtg aatggaaaaa gtgttattag ctattttaaa
    14461 gaactaaaaa tggaaaaagc aaaacgtctc gtgctagaaa atgaattaac ctatacagaa
    14521 attgcagata tattaggatt tcattcgatc catcattttt cttcagcttt taaaaaatat
    14581 ac
//
LOCUS       DAZKLA010000064        14461 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000064 DAZKLA010000000
VERSION     DAZKLA010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.065"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..315)
                     /locus_tag="ACG85N_001701"
     CDS             complement(<1..315)
                     /locus_tag="ACG85N_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0224469.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKI"
     gene            complement(315..545)
                     /locus_tag="ACG85N_001702"
     CDS             complement(315..545)
                     /locus_tag="ACG85N_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0224470.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            832..1170
                     /locus_tag="ACG85N_001703"
     CDS             832..1170
                     /locus_tag="ACG85N_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224471.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(1375..1937)
                     /locus_tag="ACG85N_001704"
                     /pseudo
     CDS             complement(1375..1937)
                     /locus_tag="ACG85N_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(2669..3250)
                     /locus_tag="ACG85N_001705"
     CDS             complement(2669..3250)
                     /locus_tag="ACG85N_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0224472.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(3433..4059)
                     /locus_tag="ACG85N_001706"
     CDS             complement(3433..4059)
                     /locus_tag="ACG85N_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0224473.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4081..5412)
                     /locus_tag="ACG85N_001707"
     CDS             complement(4081..5412)
                     /locus_tag="ACG85N_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFQ0224474.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(5567..5968)
                     /locus_tag="ACG85N_001708"
     CDS             complement(5567..5968)
                     /locus_tag="ACG85N_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0224475.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6054..6260)
                     /locus_tag="ACG85N_001709"
     CDS             complement(6054..6260)
                     /locus_tag="ACG85N_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224476.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(6355..7857)
                     /locus_tag="ACG85N_001710"
     CDS             complement(6355..7857)
                     /locus_tag="ACG85N_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFQ0224477.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7875..8330)
                     /gene="argR"
                     /locus_tag="ACG85N_001711"
     CDS             complement(7875..8330)
                     /gene="argR"
                     /locus_tag="ACG85N_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0224478.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(8428..9045)
                     /locus_tag="ACG85N_001712"
     CDS             complement(8428..9045)
                     /locus_tag="ACG85N_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0224479.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            9266..10639
                     /locus_tag="ACG85N_001713"
     CDS             9266..10639
                     /locus_tag="ACG85N_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0224480.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10714..11826
                     /locus_tag="ACG85N_001714"
     CDS             10714..11826
                     /locus_tag="ACG85N_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0224481.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11898..13535)
                     /locus_tag="ACG85N_001715"
     CDS             complement(11898..13535)
                     /locus_tag="ACG85N_001715"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFQ0224482.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13703..14311)
                     /locus_tag="ACG85N_001716"
     CDS             complement(13703..14311)
                     /locus_tag="ACG85N_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0224483.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3937 a         3014 c         2551 g         4959 t
ORIGIN      
        1 aattttagcc atgtcttgca atggtaagtt ttctattttt ttgagttttc gttccttaaa
       61 atcaagagat tttaattgtg taataattac ttgtcctttt atatctagct cttcaggtaa
      121 tgagtaacgt gtcggtaaat tctttatagt ggaagtaatt ggacatatga ccgaaaacat
      181 tgttttacgg ttaaattcat aacgagagac gactagtcct ggtcgtcttt tttgaatttc
      241 ttttcctacg gagggatcaa aatcaatcca tacaatatca ccttttttag gaatataaat
      301 tctttcttct gacactataa aatttctctc ccttcaggag ttaaatcttc ccattcgtct
      361 ttctcataat actcggcctc ttccccacca ctaaatggat cttcaatttt tggaactaac
      421 gtaattgttc catcatcaga atacacaacg atatattctt ggttttctga aggtttcttt
      481 ccgttatcag aaggtaaagt taccactaca gaacttcctt gtaatctaga ttttgtaaca
      541 agcattatca tcactccctt aataaaagta tactatataa cactaagtaa cgcaaaattc
      601 ccctttgtaa actatctaga aaattgtttt gtttaaagta tatcttattg gatataaata
      661 gaaataaatt gtagccagtt cattttttct ttaactaaaa aaataatcca agaaacattg
      721 aacctatgga tccaaaagct acccaaatac tatagctaac cccccgctac cttcaatttt
      781 gcagataaaa cgctttggga gaaaatttca aagacaacag gccatcctat catgcccgat
      841 tataaacaaa aagctggcta ttccttttgc tctgacaaat ttcaattggg aaagttcacg
      901 aaactagcag gtgaagaagt ttcaactgtt cggatcaaag aatgcccgct tcaaatagaa
      961 acattggttc aacgaatttc aactagacaa gactttgcta ttgtagaacg tgagatcgta
     1021 gctatttttg tagaagaagg gattttacat gatccaactc atatcaatgt ggataaatgg
     1081 caacctctta tctataaatt cagagaatat acaactgcca accagtcgtt aactttgaat
     1141 tttagattcc aagaattcaa agagtcataa tgataaacat cgttaggtat aaataaaaaa
     1201 gactcgcagc acgagtaaaa aacaaataga agctatacat tagaaaggtc gactgtgacc
     1261 ctatagatgc agtatatgca gcttatgagc atttcttctg ttctctttgt ttttgggatc
     1321 tgtgctgaag tctttttcgt tttttgccgc tctttctttg aaaaatcgat tatgttatac
     1381 cttctccatt actaattcag gcatctgctg agtaatgatt tttcgcgcaa aagaaatatc
     1441 tgttacccaa ggttcgattt gttcttcagt caagacaagc ggcattcggt catggattgg
     1501 tgcgactgtg cggttaggtt cagtagtcat tatgattgat tctgccattt tttcatcctg
     1561 atggctacga taaaaaccgc caatatacac tattttttcc ttatttgaaa acataaattt
     1621 gtctttttct tcattccact cattgggaaa acacaacgat tttgcaaaaa tggttgatga
     1681 aaggtctttt tttcttctac tgtctctgca cgggcattaa tcatcaattg tcctttttta
     1741 aatccggtaa atccccactt catgatttcc ggagtgagga tgttttcttc ctcttttctt
     1801 aacgtcacaa caagatttga cggaaaaact tctccagctg ctaattctgc tgcctgcttc
     1861 ggttgtttcg tctgggcttc tcgataataa ttctgaagga gctctgactc tagatcaaaa
     1921 aaataacgac cacacatcct aatccctccc aatctttttt tctatcatac actgcaacaa
     1981 gaagttcgca ccaacaaaac gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg
     2041 acaaactctc cagtgaatgg tattctcctg ccaactcctc ggtcacaagt cacaatattc
     2101 aacaaacatg agaagctgct ttttgaaatt gttccttgtg actcggagtg aaacggcagg
     2161 cttacgacct ctgggtaaac ggtgttcaaa agccagtttc tcggtattaa gccaaattta
     2221 tgcaaaaatg tagaaagcta ttttcacaaa atttttctta atactcgaaa ctaagcgact
     2281 ttttcacaac ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccaa
     2341 ccaaacgaaa aatatgagca gctattttta tttggctgtt cttattactt gatgcagggc
     2401 agctttttca caacctctcc actaaacggt gttcaatcag ctaaccttcg gtattagctc
     2461 aaaaaacggc aaaatatgag gaactatttt gccgtttgtt tctctaatac tcaggtcaaa
     2521 gcgctgattt cacaacctct aatacacggt gttccagaag tagcttcttc gaaaaataag
     2581 ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
     2641 tgaccacttc tgtcacaacc tctaattatt aaagaacctt ttgcgaagtt aacaatcttt
     2701 ctttgatcaa ttgaaaattc tgcctaagta gtgatagatc ctgtgtgact ttcaacaata
     2761 agatacttcc ctggatttca gcaatcaacg ctttggcttg acggtctgct tcttcatcgg
     2821 acagtttttc ttcagaaagt gccaggatat tttgtttcgt atacttgacc cattgattga
     2881 ctaattctag tagcaacaga cggtattctt catccactgt actcaactca ataatcaaat
     2941 tacctacagg acagccatga taagtgatct gtgtctcatg atcagcgaaa atccagtcca
     3001 acatttcttc taatgcttct tctggctgtt ttccagcatt taagatgtct tcgaacaatt
     3061 ctgtctgcca tacgtccata tgcgcatgga tcaccgctag acaaacttct ttttttgaag
     3121 aaaagtaata ataaaattga ccttttcctg tatcagaagc tgctagaata tctcggattg
     3181 acgtttcatg atagcctttt ttataaataa gatctttggt cgcttccaag atcttttcct
     3241 ttgttttcaa tgatattctc ctttttcatt tctcatagtc ctatattata cagtaaattc
     3301 atcatttttt cttcttaatc gtttaaaaaa gactgtgcca aattatctga cacagccctc
     3361 actataaaac tgttctcata tagctagatg ttcattgatt caagaactgc gaggacaaaa
     3421 gcaattccta tcttatgata gttgctctaa tacttcctct gttgtcatca cttgatctgc
     3481 aataaagcca aagttcgtca acgctgcatc cattgcttct tggccaggag caccaattgc
     3541 atctgtcgct accacaacat tgaatccact ctctaccaat tcacgcatat gggaatcgct
     3601 acacaagttt ccgttcattc caccaagaat tactgtgtcg attccatttt ttctcaattg
     3661 gagcgccaaa tcattgcttt ctggtccaaa gattttatga ggagagctga tgatcgtatt
     3721 ttcatccaaa taaggtttca attcttcgat aaaatcggct cctgaacctt cttccactga
     3781 ttcatattga ctttttctag caaacatctg ctgctcaagc atcatatgtt ctcctgcacc
     3841 gccaaattgc catttatggt catgtgggta aaaataatga ggagaaataa agagtttgta
     3901 tcctttttct tttgcttgtt ttaccatttc aatcatattt tcgatcgtat tgtatttttc
     3961 taagatatct tttgtcaaac caaagccacc gcctgctggt ttcaagaact cgacttgcgg
     4021 gtcagtgaga actactgctg tatttttatt attgaacatg gtggattctc cattcgttat
     4081 ttattttaat acatcattta gagcttcact cattgtcgga tgagaataga tttgatctct
     4141 caataacgta taaggaagtt ttccattcat agctagcgaa atcaagttga tgacttcatg
     4201 agaatcttcc gcatagatac ttgcccctag aatcaagtca gtttcagggt cgactaaaac
     4261 tttgaataca cctttaggat cttctaatac ttgtgcttta gggacaccgc ttgtcatata
     4321 cttgaataat ttgtaattga cgccttgtgc tttggcttgt ttttctgtta aacctacatt
     4381 ggataaagtc ggagtgataa atacactgta aggcacagct ttccggttat tcgttgttcg
     4441 tttattctcg cccttcaact gatccataat gattcgataa tcatctagag agatataagt
     4501 gaactgcaat ccgcctttga cgtctccaat cgcccaaatg ttgtctgcat ctgtacgaag
     4561 gtattcatcc acagcgattg ctccgcgatc tgttagttta acatctgtat tttctaatcc
     4621 aagtgatgct gtacttggtt ttcttccagt tgcagccaaa acttttgacg cttggattgt
     4681 tacttcttgg ccatcttctg taaaggtgac ggcagctgtt gattcttggt ctgctacttg
     4741 atctacagat acacctagat ggaattcgat tcccgcattt gtcatgtctt ctaaaatcat
     4801 ttgcgcgata tcttcatctt cacgtggaag aaattcagaa tgggcatcaa gaacgacaac
     4861 tttagatccg tattcattga acattgatgc aaattccaag ccaatatatc cagccccgat
     4921 gatcactaat ttttccggta attgttcctg atccatcgct tgcgtagaat caatcaagta
     4981 gcgacttgta ttcaatcctt tgattggtaa aatgattggt tcagaaccag tattgatgaa
     5041 aatccgttcg ccttcaacaa tcactttttc accattattc gtcacttcga tttgatggtt
     5101 agaaagaaaa cgtgctgtac cgtccatcac tgtcccagtt gcctcatcgc tgatcatatg
     5161 ataatttttg gcgcgaagca tgcccgtcaa tttttctttc cgagcaacag cctctgtaaa
     5221 gtcgattcct ctttctccat tgaaaatcaa tgatttagaa ggaatacagc cgatgttgat
     5281 acatgttccg ccatacatac gtgttgactt ttcaacgact aaaaccgatt caccttttga
     5341 agctaacgcc ttcgctaaag tcttaccgcc ctttccaaaa ccgatcacaa tattttgata
     5401 tttttccatc atttttccct cgcttctttt ttacctaccg attggtacaa aaatatgata
     5461 accgataatt gtctattctc caaatcatct gctttatttg tcaaaaaaaa ccagacaaaa
     5521 agcggtcctt tcacgttttt agtctggttc gatttccttt attcttctaa acaatcgtta
     5581 cttctacatc gatatttcct ctagttgctt tggagtaagg gcagaattca tgcgcatctt
     5641 ctgcaatttt ttgggcttct tcttcgctga tgccatcgat atgcacttct aatactacac
     5701 cgatctgata gccgccatca tctaaattat aaagagagac acgtgctttt actgtggact
     5761 ctgctgtcat tcctgcttct ttgatcatat gttgtaccgc cccattgaaa caagaagcat
     5821 aacctgctgc aaataattgt tctggatttg ttccttctgc atggattcct ggaggagtga
     5881 ttttcatatg catcgagcca tttggtgcct cgacttctcc attgcgcccg ccatggttga
     5941 tcattgctgt ttcgtatgct tttttcattg tactgctcct ttcgattgac catttgctac
     6001 ttggctataa ttatagtgta tgacaagtaa ggcgctttct acaaaaattc tgcttattcg
     6061 aataccagaa tgccgtcttc tactattttt ccaaaaaaca aaaaaatact actcgtgaac
     6121 aacagcgtca tatttgttcc aaaataaata gcaatctttt ctacataatc ttttatatga
     6181 ttgattttaa gatacatata taccagaaag ttgaccccat aaaccaatac tgttaaaaaa
     6241 ataaccatat tcgttaccat tttttcctcc ttttgctggt ctccctacca gcactcgatt
     6301 taaccatacc actttttttg ccgtccaagc aactagcctt tcaaaaagac tggtttattc
     6361 tgctgttaat gattcaagat cagaaaaatg tccgaataat tttgctaatt ttactaacgc
     6421 caacttgttc tttccgaatt catcattttc tatctgtaat actagtaacg gctcatgtaa
     6481 gcttaaacgg atcaaaaacc agccgtttcc gtatggctct tctagatcat atcgaatacc
     6541 ttcttcattt tccgggtcga agttcatccc tttttcttct tccaacagtc gcttcatttc
     6601 ttctaatatc ttgtttcctt gttcgcgtgg ttcatcgctg aacaatttta atcggatttc
     6661 ttgtacttct acgggctgtt tcaactcttt gatttgttca tctaatgtga taccttttct
     6721 ttttaattct ggcaaaagca tcaggatctt tgcgatgaca tatgcaccat catccagaaa
     6781 atagttttcc ttgaatgcag cgtgtccgct tgtttcgatg gctagctgtg tatctttccc
     6841 aagacggtta agttctaatg ctttattgat tacatttcga tagccggaaa tataacgaac
     6901 ttggatgcct cctttttctt caatgaatct ttgtagatga gaagaagtag gggaattcgt
     6961 cacgattctt gttcctggat gctcttttaa tacgattgta cttaatactg cgatcaaatt
     7021 atttcgattg atcactgttc cagaagcact gacaactgca gaacgatcca catctgtatc
     7081 gaaaatgatc ccaagatctg cttcatttgc taaaacagcc tgcttgatac tttccatcgc
     7141 ttctttatta tcaggatttg ggatatgatt aggaaatcgg ccatctggtt ccaaaaattg
     7201 ggaacctgtt gaatccgcac ctaagctatt caatactttt tcagcaaaga atccaccagc
     7261 accatttccg gcatcaacaa tgattttcca accatttagt ggtttgcttt ctagtgttcc
     7321 aatccccttt ttaatttttg tgactaaatc ttgcgcataa ggtgtcaaaa ggtctgcttt
     7381 ttctacgctt ccagtttgtt cgctttttat tttttgctgc tgtgatagga taaaagcaat
     7441 atcttctttt tctgctcctc cttctgaggt aaagaatttt attccattat aataataggg
     7501 aagatggcta gcagtcagca taaccgcagc atcgcacgag aactgttcaa attgagtgga
     7561 cataaacata gcaggcgtag tagctaattc gaaatcgatg acatgtatcc cttgttcttt
     7621 cagactttgg atcaacgcct ctttcagtgc tggtccgctt atcctactgt catgaccaat
     7681 cccaatcgtc cattttttat ttggatcggt tgatttcttc tttttcttta tccagtttgc
     7741 tagtccccag ccgatctttt ttaccatgtc tgtcgtcaag tttacttcat atccttcaac
     7801 tggtaatgcg attcctcgaa tatccgatcc attttgcagc gctttcatat ctttcatata
     7861 aaagtcctcc tcatttattc cctcattttt tccagctttt ctgcaaatcg ctgtgcttct
     7921 tcttctgagc ggcaaataat aatgaatgta tctacaccgg ccatcgttcc gacaacttca
     7981 gggtaagcca cttcatctag ccaattcgtt accacatcgg caccatttcg ttcagtcaat
     8041 acgataacca tgaactgaac agactggata cgcaaaacct cgcgtctaat tgctgtacgc
     8101 aaacgttctt catttaaaga aacagatggc tggttaaata atgtatagcg tactattttg
     8161 ttttcatttt gactttttac taacttcatt tctcgaatgt ctcgagaaat cgttgcttga
     8221 gtcacatcgg cgcctgcttc atgcagccgc gcaagcagct cttcctgtgt cccaataggt
     8281 tgttctgtaa tgatctggcg gatcagtgct tgtcgctgtt tctttttcat attagacacc
     8341 tctcaatgtt gattatagca aaagataagt aatatttctt catattttgg tattttttac
     8401 ttttattttt tgttttcatt caggttgcta acctttttct attttaacac gcaaaatcgt
     8461 cttcattttt tcaggtgctg attttcttgg atcgctgatg taaatctctt tgtgttcttt
     8521 agaagctctt tcatagcctt cttttgcgat gaatgcttct aatttctcaa acgttgctgg
     8581 ttcttcggca tagggcccga cgtgtaagat ttgggcacaa agaccttctt ccatcttttt
     8641 gaatactact tgttctttca agtcttccgg aagtttgcta gaacgctgaa tggcttcttg
     8701 tgctatcgct tctgtaacaa agtcaggctg tcggatcatc aattgataag aaaaatgttc
     8761 ttttttcatc actggttcat gcaaatactt ttcttgcagt ccccattgtc cttccagtgg
     8821 atagacagta tacatctgat aatcaggaat cagccaattt tttttaggcg acatgcgtat
     8881 tgcatagctg actgcatata gacaggctac tcttcgttta aagtcttcct gattcgggtc
     8941 gccgatccct ctgatgctaa agaatgattt tttaggaacc tctagtattt gcgggctttt
     9001 tttcatacta tagctcttgt ctttacgcca ttcaattttt tccatttttc ttcctccgcg
     9061 aaattccatt tagttattta ttatcatacc tctttttgag tgacttggct aatcgtgttg
     9121 acactatttt tcagtttgtg ctagaataag tgaagtaaat aaatcgtaca gataacacgt
     9181 ttcggcgtgt cgagagagag gcggtttcta tttttataat agaaatcact ctcttttttt
     9241 acgaaatttt ttagaggtga tgtaaatgac aaaacaaaaa cagctgcttt tagtacctgc
     9301 agtaattgca acagggatga tgtcttttgc gggcgtattg atcgagacgg ccatgaatgt
     9361 aactttccca actcttatca gacagtttga tttaacgact gcacaagtcc aatgggtaac
     9421 aacgatttat cttttgatga tttcgatcat tgttccgctg tctacttatt tgaatcgcaa
     9481 ttttagtctc aaaaaactct ttttagcttc aaatctgctt ttcttggcag gtataatgat
     9541 cgatttcttc tctcctacct tcatggtcct attgtttggt cgattgctcc aaggtgcagc
     9601 gacaggtatc gctcttccgt tgatgttcca tattatcttg acatttgccc ccctcaatcg
     9661 acgaggcgcg atgatgggtg tcggtacttt gacaacggcc attgctccag caattggccc
     9721 cacttatgga ggcattatga cttcacattt tacatggaat catatcttcc tgtttcttgt
     9781 gcccgttttg tttctttctt tgattatcgg ctggtttgct attcccgaga tggcagtaca
     9841 aaaaagtggt tatttggatt ggataagcgt tttaggaatt tgtcttttgt ttagtggaag
     9901 tctaacattt ttgagtacgt tgggaacagt ttccagctgg attgctttaa tcatcggcgg
     9961 tattggctgg ttcctttttt actatcgaag taaaaaagtg gagcatccac tgatccgact
    10021 gtctattctt tcaaataaaa cattccgctt gtttttgatt ggcttcttag tttatcagtt
    10081 tttattactt ggtgtatctt ttgtcttgcc gaatttcctt cagatcgttc aaggaagtag
    10141 tccttttgtc gctggattag cgatgcttcc cggcgcagcg gttggtgctt tgctttctcc
    10201 tttttccggg aaaatgctgg atcaatatgg tccgaagaag ccaatttttg ctgggctgat
    10261 tctttctttg attggttggc tagcattatt cctactcata ggacacgctt cagtgggtgt
    10321 attgattgct tgtcatgtgt tttatatgat cggtatcgga ttatcttaca gcaatatgat
    10381 gaccaccgga atgaatgcac tgaatgaaga actacaagga gacgggaacg caatgttcaa
    10441 taccttgcag cagttttctg gcgctgtggc tacttcatta gtagcggtca ttatcaatta
    10501 cgtccagcat catacttccc ataactatga agtgtctact acattaggat caaaagcagc
    10561 attaggcgtc ttgttgctgt tgcttctcgt aagctttgct cgattcgcct attatctgtt
    10621 ctttgctaaa aaagcgtaga ttccatatac ggcaggtgtt gcatctgccg tttttttgtg
    10681 tgaaaatggt tgtttgtaaa aggaggcttt tttatggaaa aaattgccat tatcggtggc
    10741 gggatcattg gtatgaccct agccagttac ttagatacat caaaattcaa tgttgcttta
    10801 tttgatgaag ggacaggaca ggcaactaaa gctagcgctg ggattatctc cccttggttg
    10861 tcgaaacgca gaaacaaaca atggtatcgt ctggctaaag acggcgctgc ttttttccct
    10921 aaacttataa aagatttcca acttgatcaa acgatttacg cacaatcagg gacacttttg
    10981 cttcgacctg aaaaagattt agctgaattg gctgttcttg ccgagaaacg aaaagaagaa
    11041 gcgccagaaa ttggaaacat cgaatggctt tcacctgaac agacatctca agcacttccg
    11101 ttattgaaac cagcagtttc actgcgtatc agtggtggtg gtcgtctgga tggaaaagca
    11161 tatttagaac atcttggtca gcgtttagca caaaaacaaa tggagcagat ccaggcaaaa
    11221 gtcactttgc ataagaagag cgattcttgg ctcatcaagg gaccgtttgg agaatacaca
    11281 gctgatcgtg taattttgac acctggcccg gcacttaagc ctttattaca agaattaggc
    11341 tatgctgcag atatacgtcc gcaaaaaggg cagctcttag catttcagac atcttataag
    11401 atgagtcaag actggcctgt tgctatgctt gacggagaag ctgatttcat tcctttcatg
    11461 gatggaaaga tccttttagg tgctacccat gaaaacgatc aaggctggga tttggctcct
    11521 actagcgaag cttataaggg attgactaag agtacagaac agttcttgac tgatccagat
    11581 cagataaaaa actggccata cgagttccgt gtaggtaccc gtgcttatac gtcagatttt
    11641 tccccatttt tcggcgtact gccagatgat ccaagcttac tggctgctag cggtctaggc
    11701 tcttctggct tgaccacagg tccttatatc ggctatctat tagccaactt cttaaatact
    11761 ggtcattggc ctacggaaga ttatcaaaag gacatctcca cctatattac gaaaaatcca
    11821 tcatagaaaa acagcttttt caagtaagga tcccgagcga tattgcgcat ggattacttc
    11881 ttgaaaaagc tgttatttta gttcaatttc tgaatcaaat tctccaaact ttcttttgct
    11941 tcatctggca agagatcttt tcctatctga gcttcaatga atcgtaaacg aatatcaagt
    12001 cgttgtttca ccagattttt ataggacgta tcctcaaaag aaacaggaaa atctggtata
    12061 ggtaaatagt ggatgtcttc aaatggacca aacgacttga attctgccgt ttgttgcacg
    12121 attctttcaa cgattgacaa tgtcgtttca ggtttgattt ttttatcatc gaaatatcca
    12181 gtattcagga catagcaggc tgtttgctgt ttcaaaaaca actctcgaaa agcttgatag
    12241 tcttccctaa gaggatagac acggaaagga ttagcaaatg gttcaatgac tagctcatct
    12301 agctgtcttt ttgtagtgat attttccgcg tctgatcgtt ttgttgctaa tgttgcacca
    12361 aaagacgctg ctaaaactgg gtcattgatc ttgataattg gcggaagcga atcatctttc
    12421 atgatccaaa aaacagcatc gatcttttcg ctaagatgat cgactcggtc gggtgtcgca
    12481 taacgtgatt tgactgtccg cccatttccg tttcgtatat ctcctgttac taggactttt
    12541 tctcctttac ttgtacatgt cacgcccaca ttctgacaag taaggaaaga agaaatggcc
    12601 ggatcatcag acggatagtc ttgtgtttta tcaaagtaag aaggttccaa ggcagtagca
    12661 aaaccatttt ttcgagaaat aacgaaagca tcgtcatgca agatcgaaac agagtgacct
    12721 tccgtatgtt ttgctaaggt aatcgttgat tttccagaac cggacaaccc aaatacagcc
    12781 atcgtatagg tctggtcatt actagcatat tgtttcatcc caccgtggca ggcaataaag
    12841 ccattgcgat ttcccaatgc ccaagcaagc gtcaaagtcg ctttttttaa ttctccaaag
    12901 taacgtaatc ctagcacagc cgctgtatta tggagagcat caaacaaaat gagtccattt
    12961 ggaaatgttt catgtgtcca ttcgggatca caaaagatat aaagatctcc ctcatcaaag
    13021 gaaacagact tttgataagt ctctaagatc ttcgaattcg ctggctgaaa attcagcaaa
    13081 taagaataca aattgttctc aaatccttct ggaatcagca aatgactttg cacagaaaat
    13141 tcttcagata ggccaacgat tacttttgta tggtaaaact ggcgcttgcg actttgatac
    13201 acagcttctc ttaaaatttt ctcgtatttg ctttgttcct tgtctaagtc ctcaaagatc
    13261 cgccgagcac ttgctgttct tcccacaatg cttccatcat tgaatactag tgtcctaaca
    13321 tcagctggta aatctagttc ttcagcatgt gagattgtta aatctgtgat gatcgttcct
    13381 ggactccctt ttgctagttg ataagctgtc ttcctatcaa aaacttcatg aacattatta
    13441 ccgtaaaatg tcgtttctac aatcgatcga atactggata acagtggatt tccacttgtg
    13501 atttcgttta attcgagact ctcttttgta ctcatggctc ctcctcattc cctaaacaaa
    13561 aaaacgtttt ctttattata cattattcga gtttttattt gtactgctgc gctgtgggaa
    13621 gcaaacgagt tatttttcac acaaaaaaac cgagaacttg aataaacaag tttctcggtt
    13681 tcatcatttt ccttttatcg gcttagtacc agccgtttgc taaccagaat gttttagcag
    13741 catcccatga accataacga gatgttacat attgttctgc tacacgttct tgattggcag
    13801 ctgaatagtc gccgtttaag tatgaagcat ctaattgata acggccgatg taacgtccgt
    13861 tagtagctgt gtatgaaccg cctgattctt tttgtgcgat ccattctttt gcagagcttg
    13921 aagttggcgc tgttgcagca gctggggcag caggagctac ttcaactgtt tcaacaggtg
    13981 caacgtatgt atgttcttgc gttgctgttt cagttgtaga tgctgtattt tctgttgttt
    14041 tgttttctgt tgttgctgaa gaatcatttg aagttgggat tgttaattgt tcaccaacat
    14101 aaatgcggtt gatgtttgcg atcgaatttt tttcagcaat tgcatcgatc attgagttgt
    14161 ctcctgcaaa tttttgtgag atcttagata atgaatcacc tgattttact gtataaactt
    14221 catcagcgtg agcatttgta gttcctacaa acattgcagt agcaccagca gcaaaagttg
    14281 tacctaaaac aattttctta aatgaattca tattaaactc ctttttttct ccgtttttta
    14341 ttctttcgac gctattaact ttatcattta gaaatatttc ataggtaaaa gaaacatgac
    14401 atttaatgac aaaataacga attatttaat atttgtaaca tttaaacaaa tatttttttg
    14461 a
//
LOCUS       DAZKLA010000065        14187 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000065 DAZKLA010000000
VERSION     DAZKLA010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.066"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACG85N_001717"
     CDS             complement(<1..1835)
                     /locus_tag="ACG85N_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0224484.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACG85N_001718"
     CDS             complement(1832..2536)
                     /locus_tag="ACG85N_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0224485.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACG85N_001719"
     CDS             complement(2548..4857)
                     /locus_tag="ACG85N_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0224486.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACG85N_001720"
     CDS             complement(5052..5516)
                     /locus_tag="ACG85N_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224487.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACG85N_001721"
     CDS             complement(5516..6142)
                     /locus_tag="ACG85N_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0224488.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACG85N_001722"
     CDS             complement(6149..6514)
                     /locus_tag="ACG85N_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFQ0224489.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACG85N_001723"
     CDS             complement(6504..6842)
                     /locus_tag="ACG85N_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224490.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACG85N_001724"
     CDS             complement(6832..7158)
                     /locus_tag="ACG85N_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0224491.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7136..7417)
                     /locus_tag="ACG85N_001725"
     CDS             complement(7136..7417)
                     /locus_tag="ACG85N_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0224492.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(7419..8783)
                     /locus_tag="ACG85N_001726"
     CDS             complement(7419..8783)
                     /locus_tag="ACG85N_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0224493.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACG85N_001727"
     CDS             complement(8796..9365)
                     /locus_tag="ACG85N_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFQ0224494.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACG85N_001728"
     CDS             complement(9337..10524)
                     /locus_tag="ACG85N_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0224495.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACG85N_001729"
     CDS             complement(10588..12315)
                     /locus_tag="ACG85N_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFQ0224496.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACG85N_001730"
     CDS             complement(12312..12764)
                     /locus_tag="ACG85N_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFQ0224497.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACG85N_001731"
     CDS             complement(12876..13256)
                     /locus_tag="ACG85N_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFQ0224498.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACG85N_001732"
     CDS             complement(13253..13639)
                     /locus_tag="ACG85N_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0224499.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACG85N_001733"
     CDS             complement(13641..13919)
                     /locus_tag="ACG85N_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224500.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13982..>14187
                     /locus_tag="ACG85N_001734"
     CDS             13982..>14187
                     /locus_tag="ACG85N_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224501.1"
                     /translation="MLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGVSSYSA
                     FDNDRIFGVLVQCINDECNKFYSL"
BASE COUNT         3922 a         3056 c         2234 g         4975 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 ggtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttaca
//
LOCUS       DAZKLA010000066        13959 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000066 DAZKLA010000000
VERSION     DAZKLA010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.067"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..135
                     /locus_tag="ACG85N_001735"
     CDS             <1..135
                     /locus_tag="ACG85N_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0224502.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="ACG85N_001736"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="ACG85N_001736"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFQ0224503.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2412..2663)
                     /locus_tag="ACG85N_001737"
     CDS             complement(2412..2663)
                     /locus_tag="ACG85N_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFQ0224504.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2810..3433
                     /gene="lexA"
                     /locus_tag="ACG85N_001738"
     CDS             2810..3433
                     /gene="lexA"
                     /locus_tag="ACG85N_001738"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFQ0224505.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3613..3831
                     /locus_tag="ACG85N_001739"
     CDS             3613..3831
                     /locus_tag="ACG85N_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224506.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4120..4632)
                     /locus_tag="ACG85N_001740"
     CDS             complement(4120..4632)
                     /locus_tag="ACG85N_001740"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFQ0224507.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="ACG85N_001741"
     CDS             complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="ACG85N_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFQ0224508.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7085..7528)
                     /locus_tag="ACG85N_001742"
     CDS             complement(7085..7528)
                     /locus_tag="ACG85N_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFQ0224509.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7529..8317)
                     /locus_tag="ACG85N_001743"
     CDS             complement(7529..8317)
                     /locus_tag="ACG85N_001743"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0224510.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8314..9255)
                     /gene="rnz"
                     /locus_tag="ACG85N_001744"
     CDS             complement(8314..9255)
                     /gene="rnz"
                     /locus_tag="ACG85N_001744"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFQ0224511.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9432..10019)
                     /locus_tag="ACG85N_001745"
     CDS             complement(9432..10019)
                     /locus_tag="ACG85N_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFQ0224512.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10240..11553)
                     /gene="obgE"
                     /locus_tag="ACG85N_001746"
     CDS             complement(10240..11553)
                     /gene="obgE"
                     /locus_tag="ACG85N_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFQ0224513.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11841..12851
                     /gene="gap"
                     /locus_tag="ACG85N_001747"
     CDS             11841..12851
                     /gene="gap"
                     /locus_tag="ACG85N_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0224514.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12936..13871)
                     /locus_tag="ACG85N_001748"
     CDS             complement(12936..13871)
                     /locus_tag="ACG85N_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0224515.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3863 a         3126 c         2454 g         4516 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2401 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2461 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2521 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2581 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2641 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2701 aaactgaaaa aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc
     2761 gctttttata tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca
     2821 aactgaatca agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg
     2881 atacccgccg actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt
     2941 tcacggtcat cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc
     3001 tcgtgcaatc gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat
     3061 tccagtactt ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga
     3121 cttctttcca atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat
     3181 tcgtggagat agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa
     3241 gcaagaaact gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac
     3301 ttgcaaacga ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct
     3361 cgaaccaatc atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag
     3421 tcatatctat taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg
     3481 gtctcaaact cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt
     3541 tacaaaccga acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag
     3601 aggtgagaat tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta
     3661 ggtattatta ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt
     3721 actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt
     3781 catgaagatc atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg
     3841 actgctaatt attcaaaaga caagaagtta aacttttacg ggggtgtgac aaaaatcggt
     3901 cacatccttt ttttcgaata aacggtgttc taaagccagt ttttcggtat taagtcaaat
     3961 tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat ttataaaaaa
     4021 atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt taaaatcgct
     4081 tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca ttaatgtaat
     4141 gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga tcaaaaatgc
     4201 gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt tgattgttcc
     4261 tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa tggcatcctt
     4321 atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga tggtttctcg
     4381 aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat aagcaacagg
     4441 acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc gtttttcttt
     4501 tgcataattt acgatttgtt tagtagcttc ccgataagcg tcaccatctg ccatcagtgg
     4561 ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg caatataatc
     4621 ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg gtaggaatat
     4681 cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgtcgctgg tataatctgc
     4741 tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg ttctctattg
     4801 taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt tcagggatac
     4861 gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct tgagaatgga
     4921 tcaatttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa taggcctcat
     4981 caggtgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa acttctttca
     5041 gatccgctaa atcatctgga caatccataa caactagaga gtttggtttt cttttttcaa
     5101 ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg ataatctcag
     5161 gcacgaattt caacgtcgaa gaatcaaatg ctaaataaag gctaccttcc gacgttcggc
     5221 tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc acagaaaaat
     5281 cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa agctttccta
     5341 ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca aaagcgaccg
     5401 catcaagctg actcgatgca tcggaaagga caaatttcag atgctgttgg tttgctccga
     5461 ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa ggattatccg
     5521 taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact tcatttggca
     5581 gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga tgatcgacta
     5641 tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc gttaaaccga
     5701 ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca agcatttcaa
     5761 aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct tcttttaaag
     5821 taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca atgcccaaaa
     5881 cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta ttgaccatcg
     5941 ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg atctcattca
     6001 atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac gagacggcag
     6061 gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa aaaccgatcg
     6121 ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc tgaagtccta
     6181 tccgttctga atgatgaatg gcatcgattc ctaatgaaac aaggattcga ttctcatcag
     6241 tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat tctgcaggcg
     6301 gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc aaatctggaa
     6361 atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct ggtaaattct
     6421 caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga gcgtatgcta
     6481 ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt ccttcttcga
     6541 tcttttcttg gtataccgat tggtttggtc catacccatg ctcaaatcga tttggcagga
     6601 atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt gcactggtga
     6661 tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt acagcttctt
     6721 ggattcggct gattgcttta tccatatcgt gtatcagata cggatcatgt aattcttgtt
     6781 cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt tgccacagta
     6841 acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag gattcagaag
     6901 gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca ctcctaactt
     6961 agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa aaacagatca
     7021 gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt atgaaatatc
     7081 cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt tcttccggcg
     7141 ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa gcagcttcca
     7201 actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct tcaattttct
     7261 tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt tgcttttttt
     7321 gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc agcgcagaac
     7381 cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt acaggaacat
     7441 ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat ccaagaatca
     7501 cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg ctggctaaat
     7561 aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa ataaacggtt
     7621 gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc aacttggcag
     7681 gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca gccttatcga
     7741 agaaatttgt ctcgattgga cctggattca ctgtcgtaac agctacgcca agtggtttca
     7801 attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca gaataaaccg
     7861 ttgattttgc agttgccatt tttccagcca tagaggcgat attaatgata tgaccttgtc
     7921 ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct aagatgttga
     7981 cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg aaaatcccaa
     8041 aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc ttttcatata
     8101 attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc tctttgccgc
     8161 tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt gcgcaactta
     8221 taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga ccgcctgaac
     8281 tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct cgcgaaatgg
     8341 aatctcaatg ctatcaaaat ctttcataat cgtcgtgttt tcaaacactt cttgtgcttc
     8401 tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag taagtagcag
     8461 ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg aatggaaata
     8521 gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca gaatatcgct
     8581 attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa tagtcacgat
     8641 tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc catcttctag
     8701 ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa tacctaattc
     8761 ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac gataaccaaa
     8821 actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat cagtaaaaat
     8881 cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg tttgcgaaat
     8941 acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct ctaatggcgt
     9001 atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa tatgatcgcc
     9061 gtgtaaatgt gtaatgaaaa ttttaccgat ttttctcgga cgaatagtcg ttcgcaaaat
     9121 ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct cgtctaaaag
     9181 tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac ctgttcctaa
     9241 aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt ttagggtaaa
     9301 aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa cgttctaaag
     9361 cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt gagatcctct
     9421 gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt ttgcggatgt
     9481 ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc tttcgcatca
     9541 tgcacatctt tttcgacatc ttcataatcc aagcgagtaa tttctccgcc taattccatc
     9601 attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat cagtcctgta
     9661 gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc ttttatttct
     9721 ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa aatccctagc
     9781 ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga tttgttatta
     9841 ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg atcatggctg
     9901 acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt gatttctgaa
     9961 aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg tttgctcatt
    10021 aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgaa aaataagaaa
    10081 agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta tcacagctcc
    10141 tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct cccttaaatc
    10201 tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa attcaaattc
    10261 aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa gagcttcatc
    10321 cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat gatcgaaatt
    10381 cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg catctgaatc
    10441 acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag tttcttcttc
    10501 cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg ctgtagcatt
    10561 caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg ggatgtcatc
    10621 agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta gattttccgg
    10681 tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta gcaagcgtaa
    10741 gttataagta gataattctt tgttgatggc aagatagtct tcataaggat ctcgtccttc
    10801 catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat gacgaaggaa
    10861 ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca agtctgctgc
    10921 ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttaggtacga gcgtcgtgaa
    10981 atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag tcgatttccc
    11041 gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta gttcgatttt
    11101 tcgctcttgt cctggttcac cgttttcagc aatttccggt gcaggattcc gaggagaggc
    11161 aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg tttgaccatt
    11221 ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg ttccttgtgg
    11281 tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca tcccattttc
    11341 acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac gtaacccttc
    11401 atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg gtccgccgtc
    11461 aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc cagctttcac
    11521 atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt attcatttat
    11581 ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta tcggtgaaat
    11641 ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt ttatcatttg
    11701 gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa tattctttgc
    11761 gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac agcaccccta
    11821 ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt gggcgtattg
    11881 gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta gtagcaatca
    11941 acgacttaac aagtccaact atgttagcac aattattgca atttgactct actcatggta
    12001 cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc gaagcaactc
    12061 gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac ggtgtagata
    12121 tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg catttagatg
    12181 ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc atcgtctata
    12241 acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt tcatgtacaa
    12301 caaactgttt agcacctatg gcttatttct taaataatga atttgggatc gaagttggta
    12361 caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga cctgtaaaag
    12421 gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat tctactggtg
    12481 ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa ggacacgcac
    12541 aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg aaaacaaaag
    12601 taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat ccttcattcg
    12661 ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa ggttcgatct
    12721 ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt aaaactgttg
    12781 catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta gaaaaattcg
    12841 ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat ccagtcaaag
    12901 gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg gcttaaaaat
    12961 tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc atgctcggct
    13021 ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt atctaacatt
    13081 tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg ctggtttttc
    13141 ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac ttttgtcact
    13201 agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc tagctgataa
    13261 gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt cacattatct
    13321 ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac accgtataga
    13381 acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg tacatagtct
    13441 ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg tcctcttgca
    13501 tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt ggtcatcgtt
    13561 tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt cgtttcagca
    13621 ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca atcgcttcgg
    13681 ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg tgttgtacca
    13741 ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt ataaaaataa
    13801 ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag gattttcatc
    13861 caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg aattagtata
    13921 atcaaagaat ctctactatg caatgttcat ttttataac
//
LOCUS       DAZKLA010000067        13924 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000067 DAZKLA010000000
VERSION     DAZKLA010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.068"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..175)
                     /locus_tag="ACG85N_001749"
     CDS             complement(<1..175)
                     /locus_tag="ACG85N_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFQ0224516.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLA"
     gene            complement(654..1373)
                     /locus_tag="ACG85N_001750"
     CDS             complement(654..1373)
                     /locus_tag="ACG85N_001750"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0224517.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1422..3068)
                     /locus_tag="ACG85N_001751"
     CDS             complement(1422..3068)
                     /locus_tag="ACG85N_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0224518.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3247..3837
                     /locus_tag="ACG85N_001752"
     CDS             3247..3837
                     /locus_tag="ACG85N_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0224519.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3929..5434
                     /locus_tag="ACG85N_001753"
     CDS             3929..5434
                     /locus_tag="ACG85N_001753"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFQ0224520.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5518..6870)
                     /gene="celB"
                     /locus_tag="ACG85N_001754"
     CDS             complement(5518..6870)
                     /gene="celB"
                     /locus_tag="ACG85N_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0224521.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6870..7388)
                     /locus_tag="ACG85N_001755"
     CDS             complement(6870..7388)
                     /locus_tag="ACG85N_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224522.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7404..7730)
                     /locus_tag="ACG85N_001756"
     CDS             complement(7404..7730)
                     /locus_tag="ACG85N_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFQ0224523.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7743..9722)
                     /locus_tag="ACG85N_001757"
     CDS             complement(7743..9722)
                     /locus_tag="ACG85N_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0224524.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9743..10057)
                     /locus_tag="ACG85N_001758"
     CDS             complement(9743..10057)
                     /locus_tag="ACG85N_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFQ0224525.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10225..10518
                     /locus_tag="ACG85N_001759"
     CDS             10225..10518
                     /locus_tag="ACG85N_001759"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFQ0224526.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10596..11708)
                     /locus_tag="ACG85N_001760"
     CDS             complement(10596..11708)
                     /locus_tag="ACG85N_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0224527.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11861..12709
                     /locus_tag="ACG85N_001761"
                     /pseudo
     CDS             11861..12709
                     /locus_tag="ACG85N_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13062..>13924)
                     /locus_tag="ACG85N_001762"
     CDS             complement(13062..>13924)
                     /locus_tag="ACG85N_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0224528.1"
                     /translation="IDGLAPGAYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKA
                     NRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVY
                     QLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQG
                     AVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIE
                     KEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFI
                     SIKFRKKRNI"
BASE COUNT         4341 a         2844 c         2387 g         4352 t
ORIGIN      
        1 tagccaagaa cccatctgct gaaatatgaa aatttttgaa tgctacgaca ttcgccatta
       61 caggtatgta aaagaaaaaa atcatccaaa gcatacccgg tacagccatc aggacaagtg
      121 ctttataacg ccatagattc ataaaaaaat gattgaaccc ctttcgtttt ttcatctaaa
      181 aacccctttc gattcattcg caacgtaatt atagcgcttt caataatgag aataaataaa
      241 caaagtatag ggaaaatgaa acaaagtata ggagaggttg tgaaatcagc gttttggcct
      301 gagtattaga gaaaaaaacg ggaaaatagt tcctcatatt ttgccgtttt ttgagctaat
      361 accgagggtc agctgattga acaccgttta tcagaggttg tgaattcagc gttttggcct
      421 gagtattagg gagatcgtgt ccttactatc taactctgag tcacaaggaa caatggggac
      481 tgtaacaaaa gtcttgtcac agtcccttat tccgaacaaa cggtgttcca gaagtagctt
      541 ctacggaaat aagccgaaat ttcacaaaaa agccatccga aagtcagacg gacaagtctg
      601 cttttcagat ggcgctacaa ctactctgct tattctttta gctatccatt caattatttt
      661 tctctcactt gttctagctc ttctttggtc atgggtcgat attgcccttt attcaaatct
      721 ttaggcagcc aaaattttcc catacggatt ctttttaaat ataagacttc ttttcctact
      781 gctttgacca ttcgcttaac ttggtgaaac tttccttcat gcaaaatcag acggataatt
      841 gaagtttctg tctcttgatc tacttgatca atttggagtt ttgccggcaa tgtctgctca
      901 tcgccatcaa taatcagtcc ttctgcaaat aaacggatat cttcttctgt cattataccg
      961 ctaacctcag ctagatattc tttctccaca tgttttttag gtgatagtaa ctgatgggca
     1021 aacaccccgt cattcgttaa aatcaagagg ccctctgtgt ctttatctaa tcggccgact
     1081 ggaaaaagat cttcccgata atcctggtct tccaataaat cgattacggt ttgatcatga
     1141 ttatcctgag tagcagaaat gactcctgca ggtttatgca aaatgtagta aaaatctttt
     1201 tgatacgtaa tgatttctcc taaataagta accgcatctt cgaattcatt tacttgcgct
     1261 ttatcgcttt tcaccacttg tccattgact tcgatcgttc cttttttgat taatcctttg
     1321 acttctttgc ggctgccaag tcccatatct gctaaaaact tatctaaacg catgcgtttc
     1381 ccccatttca aaagagcatc aatctagctc ttctgattca cttattttat ttttaattta
     1441 tgtcgtaaac gcgctattcc tggtcctagt agtctttctg ccaagcggat tttcaaagct
     1501 aaataaccat acactaaagc ccctatacct gctactacaa gaatcaacaa tagagactgg
     1561 aatttgctat cttcgtttaa aaaagcacca aaaatcatct ttgtgatcca cgaagccaaa
     1621 agcataatca aggtaagaat aaagatcagc aatgtcctgc gccagacaaa acttggttta
     1681 aagcgggcta cttcatggat tctctttaag atcaaggtac aagacacagc aaatccgatc
     1741 attgtcgcca ataaagggcc gtatacttca aataaacgaa tggctgggta ttgaagaagg
     1801 agtttcacta gaaacccaat aaccaaataa ttaattgccg ccttgttttc aaacattcct
     1861 tgcagcatgt tggaaactag catatatagt cctaggaaga gtcctacaaa acttgcttgg
     1921 atcagtacat tacttcctag agaatccggt gtataaaata atgtatttag cggataggca
     1981 agtaacataa caccaaatgt cgccggaaac ataaaaaatg aaaacaactg taaatttccg
     2041 cttgtcaatt ttgccagacc tactcgatct ttcaacgtaa cagcttctgt aatcaacggt
     2101 aacccagtag acgcgatcga tgttgccaat gcaataacta ccatcgtcaa cttatctgga
     2161 ttggcactaa aaatcgaaaa aagatccagt aactgtgcat tggaatactc ggtggatgca
     2221 gacatgatct tcataaaagt gaactgatcg actaatttga agatcgttac ccctgaaccc
     2281 acaataataa acggaatcgc ttctttaacg gtatctaaca acaattcctt cgttgcaata
     2341 tttattttgt tttcgctgta gtgaataaac gccgatgtat acgcttgatg tttcttcaaa
     2401 aaatacaaaa ggacaagaat acttgctagc ataccgataa atgccgcgaa agttgattgc
     2461 gtgaccgccg tcacatagtc accatccatg actttcatga tgatgaatgt agccaatagc
     2521 atataaaaaa tacgtgccac ctgttctaca atctgcgaca gcgcataagg catcatttct
     2581 tgattgcctt gcaaataccc tcgtatcaca ctcatacaag gaaaaaccaa tacagcgata
     2641 ctcaaagaac gcatcgttgg aatcagttct tctccgccac cagaagcatg agctagccaa
     2701 ggagaggcga tgtacatgaa caaagcaaac aacaggccta acccgcccat catctgtaat
     2761 gcacggataa ataatcgtcg tgaagtgcca tattcattca atgaattata gcgtgcagtt
     2821 tgttttgcaa tcgcagcagg tattcctgct gtagagatca gtagaaacaa tgcatatata
     2881 ttgtatccca tattgaataa tccattggca gccttagcat tttcgcccat ccaagcatac
     2941 cagggaatga tatagacagc gcctagcaat cttgaaatga tattactggc ggtcatccaa
     3001 gcagagcctc gtgccatttt ttcttgcggt gttaattgat ctaatgctgg ttttctttga
     3061 ttatccatcg gtcagattcc aactttctac aaaacattac tatcatttta attggtaaaa
     3121 cagaaactgg caatcgattt tatataactt ttataagaac aattgttttt ttgaattcgg
     3181 tttcgttttt ttggtaaagt aaagaaatat acagcctttt atggctaaca tttataggag
     3241 atatcaatga cacaagaaat aaaaaaacta cccgttatat ttttaggtct tttctttatc
     3301 acagttggat tgaattggtt cttgctccct cacgatatag cttccgccgg agtgggcgcc
     3361 atcggacatt tagttgaaac ggccttttct atccaacgct cagttactgt ttggagcatc
     3421 aatcttatga tgctgcttct agccgctgtc ctattaggaa aatcagtatt ttttaagact
     3481 gtagcgggaa gtctgctttt tcctgttgtt ttagagattg ttccaaaagt ggaaatgatc
     3541 tcctcacatt tcttttcact gatttcagga agtcttttgt tcagtcttgg ggtttacaca
     3601 ttgtacacag tcggggcctc aaatggcggt ataacgatac cgccgattat tttccacaaa
     3661 ttttttcggc ttccaatgcc gcaaggactt ttacttacca atagtcttat cgtctttttg
     3721 aattattatg tttttggatt gttggagaca ctattcgttc tcctatctat tacagtcagt
     3781 tcattgttta tgaaaatact catccgcttg tcccctgtgt caagaaaatt tgaataaagc
     3841 tggattttgt catagcggaa acaaacgata tattaaaaag agtgtgctat aataaaccaa
     3901 gaaattgaat aaagcgaggg atcaatcaat gagcttctct ttagaagaca tccgtcattt
     3961 actattaaaa gaaaatctat taaaagaatt tgtttcaaca agaggctggc atcttgatgt
     4021 accagaaaaa gctgaatttc ttcagttaag ttatgattca agaaaagctg atgcttctac
     4081 tttgtttttc tgtaaaggaa tgaatttcaa agaagaatat ttggattcag caatcgaaca
     4141 gggtattcaa tattatgtat cagaacaacc atacgaaaat tcagcagtag gaatcatcgt
     4201 aacggatatc cgtaaagcaa tggcgcttct tgctatggct ttttacgact atccgcaaaa
     4261 taaattgaaa gtgatcggat tcactggaac aaaaggtaaa acaactgctg cgtactttac
     4321 aaaagcaatc ttggatcaca caacaaataa aaaaacagca ttgctttcta ctatgaatac
     4381 aacgttagat ggcaaaacat atttcaaatc ccatttgaca acgcctgagt cattagattt
     4441 atatcgtatg atgtcagaag cagttgaaaa tggtatgact catctagtga tggaagtttc
     4501 ttcacaagct tacaaaacac aacgcgtata cggcttgact ttagatgtcg gaatcttctt
     4561 gaatatctcg cctgaccata tcagtccgat cgaacatccg acatttgatg actatttcta
     4621 ttgcaagcga caattgatct tgaattcaaa aacagttgtt ttaaatcatg aaagtgacta
     4681 ttttgattta cttagggaaa cagccgatct attccagatt ccaacgatca cttatggtcg
     4741 ttcggaagaa agcaattatc aagtgatccg ttcagataaa ggtactcacg gttttacttt
     4801 atcttctcat gaagatcgct tagctatctg cgatactgct tatgatattt tgctggctgg
     4861 aggattcaac caagaaaatg cagccagtgc gatcattgcc gcagcactaa caggtgcctc
     4921 cagtcaagat gcacaagacg gattaaaaga agctcgtgta ccaggccgaa tggatcaatt
     4981 gatccaatcc aatggagcgc atgtctatgt ggactatgca cataattatc taagcttgaa
     5041 aacattgctg aaatttgcca aaaatgaaca tccagatggc cgtgtaattg ttgtattagg
     5101 cagcccagga aacaaagcaa tctcccgtcg acatgatttt ggaaaagtgc tatcagaaac
     5161 agctgacatc gcctttttga cggcagatga cccagcattt gaagatccga aaaaaatcgc
     5221 ggaagaaatc aatgaagcaa ttactaatcc agatttgatc gttcattatg aaatggatcg
     5281 tcctgaagcg attcgacgcg ctttagcaga aagcgctccg caagattcag tcgttattgc
     5341 gggaaaaggc gtggatccct atcaaaaaat caatggtgta gatgaacctt atgaagggga
     5401 ttatgcaatc gtgaagcggt tgattgaaga ataatggtat ttatatacta gtggtttccc
     5461 atttctaaga aaaaaaggga ctgtgatttt ttgtcacagt tccttttttc ttagaaatca
     5521 ttttaacttt tcactagcta attctgctgc cttttctctt tcttcattta ataatttatt
     5581 atcatagaat ttgataaatg ggaaccagat aatgaatgcg atgattgcac aaatgactgc
     5641 taacacagca gctttccaat caccaccact accaacaaat gctccgatcc cgactggtga
     5701 aggccacggc atctgtgcaa gcatcggttt gacgatttcc agccgaatgg caaaataagc
     5761 gagtgtagca gaagccatcg gagctaagaa aaacggaacc gctaaatagg gattgtagac
     5821 gattggcatc ccgaaaatga ttggttcatt gatattgaat aatcctggta caagtgaggc
     5881 ttttcctaac actttcagct gatcagattt cgcgaggaat gcaataaaga tgatcaatcc
     5941 taaagtcgca cctgatccac caacggttac ataagaattg ttgaattctc ctgccaacgg
     6001 ataatttgcg ccttgtgcgt tagctgccat attagctaat acgatcgggg tcaagaaaga
     6061 agtgatgata ttcgctccat ggatccctac gatccacaat gcatggatca agaaataaat
     6121 caccatgaca ccaagccatg tattcgtcaa ctgcgtaaca aacccaaatg ggatagcaat
     6181 cattttgaag atatccgttc ctagtgcaac taataatcca ttgatgatca tcaccacaaa
     6241 cgcaatgaca aatgttggga tcagagcagt gaaagaacga gaaactcctg gcggaacaac
     6301 atctggcatt tttatgaccc aatttctctt gacacacata caatacaatt cgaccgcaat
     6361 aatcgacata ataatagctg taaagattcc tgaagttcct aaacgttcga caaaagagcc
     6421 catcctaata ccatttacta cagtttctgt ctcattgata gaagcaatca agctgatttt
     6481 accatcagtc atgatcaatt cagggataca cataaagaat gcaaaaacag atagtaatgc
     6541 ccccgtgagc ggattaacat ttaatttttg ttcatctcgt tcaattgacg ttaattcata
     6601 agcaaacaca atggcaaagt aaatagataa aatccccatc gtcattgtat tgacgatcat
     6661 gtacaagtca ctaactttga aaaaagttgc attaaaaaat ccttctaaaa atgggaacgg
     6721 taatggcaaa atatttaaaa ccaaaaacat cgagcctaca atcgtgaaag gaatagaagc
     6781 catccccgct gccatgacag cccgaacgat tttaagttgc gcaattttcc ccataggtcc
     6841 catcaggtat ttctgcaaaa aatcaaacat tagttattct cctttcctaa atataacgct
     6901 ttttcatatt gtttaatacg gatatactgt tggtcttggt tgagtgaaat tttctttaaa
     6961 cgatactgta taaatgcact ggcaaaaata ccgcttacaa caagaatgaa aacaaataac
     7021 tgtcctccat tgttatttgc cataaaagga tacaagaatg taaatcctga ggtaaacaac
     7081 actggaatca ctaataaatt aaagacactc tggaacaaaa aataccactt tgtccatgga
     7141 acgttcgttt ggtgttttcg ataaagcttt acttgttctc ctactgcagg caaaatagct
     7201 aaaatcagaa tcatcggtaa actacttcct aaacttttag ctgatagaag cagaatcatc
     7261 cattgcatgt tcatgaaaaa gaaaaaggca gtcaggtaac gtacgattag ataacgattg
     7321 aaatacatgt ttttcaaact aagttgcttt ttttctaatt cgattttcct ttttactgta
     7381 tcctgcacaa tgatccttcc tttctattta tttttttgat acaaataatt catttcctgt
     7441 gcaacttcca ataatgtcat cgtcgtcatc agatgatctt gtgcatggac catgataatt
     7501 tccatttcta tttttgtccc gctggcatat tctttcagaa gttgagtttg tgactgatgc
     7561 gcatgaagaa tctcttcatt tgcttgtcgc aacttctctt ctacttcttc aaacatctct
     7621 ttacgcatcg agcgaaatgc ttcatgtatc agtgtccgtg catttccgct atggaaaatg
     7681 atatcaaaag aagtcacttg tagtgtttca ctgtttattt tttcagccat ctgttttcct
     7741 ccttattcga tcagtttgat aaatttactg ctgaaattct caaaaatagg ttcagctaag
     7801 atttcctgct gtacttttag ctggtcaact aacctaacga ttgctttagt cacgaccgta
     7861 attccttcat tttcgacata agaaggcgaa actaaaaaaa caaaatgaat ttcttgatat
     7921 tgatcccact gcatcccaga aggaatcaat gcaacggcta cctttgtttc gattccaact
     7981 ggaacagcgg gatgtgggac tgccactgta tcactgaata tgatttgtcc catattttca
     8041 cgttgctgga tctgctttcg catttctctt atatatcctt cttcttcatt tatgctcagc
     8101 ttttgcagca aatcatgaag aatttcttcc tttttcggtt cgtacgtata agtcttgaaa
     8161 aatttttcag acaactgttc ggtgtaaatc tgcttttttt gctgtagaga aaatgctggt
     8221 atcggtctat ctgaggaatg atagggtata gattctccaa ctacctttcg tattttacga
     8281 acatcatctt cattcaaaaa aatactgaca tgtaaaactg gaattttgaa aaacattgtc
     8341 gaaagatcaa tagaagaaat aatcaggtcg attcctttta aagttgtttc attgatctca
     8401 taatagcctt ttacacttac cactgtaata tttttaccga attcactgac tacgcgattc
     8461 tttaatagtt gggcactgcc gtaacccgtt gcacagataa tcaatgcatg tagtttatgc
     8521 gcagctcttt ctttttccaa tgccgccatg agatgcaaag ccaaataggc ccattcatct
     8581 tcattgatgg tatagttttt caaagccggc atgttgctga aatgcctttt tactgcgtca
     8641 aagactttgg gattttcttt ctttatctcc tcagtcaatg ggttctcaat gcgaatacct
     8701 cggctcaatc gaatcatcat cggtgacaaa tgatcgagta gtccattgaa aagctggaga
     8761 tcctctacta atgaaaaatg aaattcctga gacaatcttt tcagaacttt cgctaattcc
     8821 tttgatagtt cctgatcatt ttgattttct ttgtgattcg acttagccat gagatgtaat
     8881 gtcaaataag caatttcttc ttcgggaaac gatacacctg cgattacctc aatacgttga
     8941 acgatccgct gcgccacttg atactctact cgcttagaaa cttcttgttc gatgcctaat
     9001 tccttgattt caaacccttc tcggagccgt ttgatcccca aagccaagtg taaagtcaga
     9061 ttctgtatga taaaatccga aacttttaac ttagcttctc ttgtttcatc gagaatgata
     9121 atgactagct cttcaaaact gatgtctggg aaaaaacggc tttgccctaa atattctttc
     9181 aatgaatttc cataatggtt tccaaaaaaa gtatccatga taaaatgccg tcgatttctt
     9241 tcttcccctt cgatccatgt cccttgacca tgcttagaaa cgatttttag tgaatatgga
     9301 atcaatcgct ccttgatttc ttgcatgtcc tttgtcaaac tggaacgact gatataaagc
     9361 tcttctgcca aatcatccat aaaaagaatg gcatcttcca aaagcaattg gttcaatata
     9421 tacttttgtc gatcttcgga ctcataaaat ccttgcggtt ctctagcata tccaggtact
     9481 atttcttgcc gactcaaaaa caagtcaaat gtcagtcttt gtaaaatatg cagttggtag
     9541 ccataacctg gtttagcaat gatcttacct ccgttttttt ctactaactc tttgatccgc
     9601 tgcaagtaat tacggattgt ccgatcagat aataacaatt cagaagctaa acgttgactg
     9661 gtgacatact ccttcttgtg atcaatcaaa tatctcaaca gcaatttttc cttctgtttc
     9721 attatcatcc actcctattc catcatatgt tctcttcgat cacctctgct aacttctgga
     9781 tgcctgtagg gattgggaca tacgcttgga aagggatact aacaacaggc tttccaacag
     9841 attctcctaa ttttttgaat ttatccaaaa acatcgtcgt ttgcgggctg atcagaaata
     9901 aatcaaaatc actattctta atcatattat cgccttctgt agctgaaact gcatctacga
     9961 gtacctcttc tcttttttgc ttaaagaaat ccgttgtctt tgaggccatc atcgaggatg
    10021 acattcctgc cgcacaaata atcaatgctt ttttcataaa tcaatcactc cttttatttt
    10081 tataaaccaa gcatatcttg taagcgtttt ctattcaaaa tatatatttc cgtagcattg
    10141 cggaaaaaat gtgtatcata tcttgaataa taatttctgg caccgtgata tactagtgat
    10201 agaatttttt caggagggga tttagtgagt atcacagtga atattttgta tacaagtaca
    10261 aatggacaag ccagaaaatt tgcagaagaa atggtcgaaa gaaacattgt caaagcgatt
    10321 cgtgcagaag aaggaaacga gaaatatgac tattactttc cactagaaga tccagaatct
    10381 cttttactga tcgaccgttg gaaagatgaa gaagcaatcg aaaagcatca caaatccgaa
    10441 atgatggctc aaataaacga actccgtaaa aagtataaat taaaaatgaa agtcgaacgt
    10501 ttcactgatt taccgtaaaa agatacacat ggaatgatag caatttatca tccatgtgta
    10561 tcttcacttt ttccttattt tttattcgat ctcatctaat gattcaaacc gtgtttttct
    10621 cttatttttc ggataagtat ttcagtcacc ccaatataca tgattcctaa acatacccca
    10681 aggatattct caaaaatgcg gatcgttact gctcctggaa ccccgaataa actggcagtg
    10741 attgccaaag caccaaaaga attaaaaact gttttatatc tataggtaga acaaacacct
    10801 aaagctagac cacctaatat accgacccag ttaaaaggaa taaattggct gataccaata
    10861 aataaaacac atccaatcaa tgagccaatg attcggtcta ctgctcgttc tttgaaacca
    10921 atcgacatca atccatagct agatacaata gaagaaaatg caaaccctgc ccacatcaat
    10981 cgctgaaaag ggacatactc tcctgcaacg atcaaaagac taatgccaaa tgcatagatg
    11041 agcatccaaa ttttttcctg cgaatacatg cctttcaaaa aaatctcttt aaacaagctt
    11101 ttgtctttgt tcttttctcg atgttttctg taaaggagaa ctgaaaacca acaaaaaaac
    11161 aaaaataata agctacttgt ttgtgtaaag aaattcttgt tcaacgaatc tttaggtaac
    11221 gaataaacaa taaagagata tgaaaagccg tacaaagaag cattccccat tttgggatca
    11281 ctagcggttg ccattaatat gctgcttagt aaaataaaat gaatcaacat gcccataact
    11341 gaccatttga tcagctggac atacggtgct actaatagtg agaacatgac gattgctaaa
    11401 cctatgatcg aatgggaaat cttataacca aagtcaacaa aacgaatact catcaacata
    11461 cagaataaaa ccacagcgta tggtttatgc ttcgattcaa ataatgttgt aattataatg
    11521 ataaaacaaa tggcaaacaa tacgatgaat aatgatcgta aaaacaatga acaaatatac
    11581 atgcactttt cttttttaga agctgcttct cgtatttttt gttttaaaat aaacggatct
    11641 agttgtagag actgataaaa atccacctgt tgtcgtcctt tctttttact tttatttttt
    11701 acagtcatct atgacaggtt gtaaatacta tggaacatca tatggcgttg tcattttctt
    11761 gtcaactatt tttcctattc atgtttttat aaaaaaacat atcgttttct tgtatgatta
    11821 aatcaccaca aaataaacaa ctgagagaac gatatgcttt atgaatggtt ctatcaaaaa
    11881 aattctgaga tcaatagaca aaaatttgat gattacagag gtctcttacg aaaccttaca
    11941 gaagaaaaaa acattgatcg ttgctgcagt tctcttccct gctccatgtg tctgtagaaa
    12001 ttgtggttct gctgtggtag atggaaatag gaaattaatg gtggtgaaaa acaagaagaa
    12061 ggaaaccatt gtccactttg aataatataa tcatatgcca atggttatac gcctaaaaaa
    12121 gcagcgctat acctgtaaaa actgtcgaag ccattggaac gctcaaagtt attttatccg
    12181 tccaaggcac tcgatttcaa atcatgtcag acataaaatc acttctttac tgactgaaaa
    12241 agtgtcgtta ttttttattt cgaaaagttg ttaggtatct ttgacaaccg ttattcgcac
    12301 attgaaagag ttgagaagct atttaccaaa gtcttccaaa agaattctgt cttgagtgtt
    12361 gagggttgtg aatttcgttc tcacgtttcc gttgaggata aactgagttt tatttccgcg
    12421 gatggcgaga cagaaaaatt aatcgagaaa atttacctac acaaaaaatt cctcgattaa
    12481 caaactattt ccttagctgc actaatccta aagaaataga attcttagtt acagacatga
    12541 acgccaccta ctttcagctc accaaacatg ttctaccaaa tgcgaaattg gtgattgatc
    12601 gattacttgg tttttccacg cctttcaagg aggcgtaacc tttattcacg agttggttga
    12661 agctttccga gacaaaagac ccgacttgtt tttctcctta ttgacaaaga gccattaaaa
    12721 ctagagaaaa aattacaact gatcgctgat tatttgtaga aagaaaatca aagtattacc
    12781 caataaactt gagtcattag gcaaaaaaca ctgcgaaact caagaatgat tttcgcagtg
    12841 tttttgtata ttttttatta actagtatta ctacaaaggt ttatttttca cttctcaacg
    12901 cagcacaaaa ttgtctttac ttatttactg gttgggttca accttctcta ctccgatact
    12961 aatatttgtt tgtatacccc ttctaaaatc ggtagtttcc attcaattga tgggtactag
    13021 ttgattttaa aaactatgct tattcaattt tttattttag ctcatatatt tcgtttcttt
    13081 ctaaatttta tactgatgaa aacaagtagc acacccaata ttgacaatat aaactggcta
    13141 atgatggttt ctccagtttt tggaagatgc ttatttccac tgcttgttgg attgtttcct
    13201 cttatatctg agctatcagc aacttttcta ttctcttttg ttaaaaagat cgccacttct
    13261 tgttcttttt cgatcttgaa agttactggt gtcgcatcca attcataccc cgttggcgct
    13321 tgagtttcta ccagctgata tgctcctggt gctaagccat ctatcgccag ttttccatct
    13381 gctcctgtgg ttagacctga ctgcagaacg gttccattgg catcttgtaa ctcaaaaaca
    13441 gctccttgca ggacttctcc gctttggtca tcgatctttg tcaacacgac accgcctggt
    13501 gtaagtcgat tttctttcgt caattctacc accgctgtct gacttctttc gatctcaaac
    13561 tcgattggtg tcgcatccaa ttcatacccc gttggcgctt gggtttctac cagctgatac
    13621 actcctggtg ctaagccgtc aatcgccagt tttccatctg ctcctgtggt tagacctgac
    13681 tgcagaacgg ttccattggc atcttgtaac tcaaaaacag ctccttgcag gacttccccg
    13741 ctttggtcat cgatctttgt caacacgaca ccgcctggtg taagtcggtt tgctttcgtc
    13801 aattctacca ccgctgtctg acttctttcg atctcaaact cgatcggtgt cgcatctaat
    13861 tcataccccg ttggcgcttg agtttctacc agctgatatg ctcctggtgc taaaccgtca
    13921 attg
//
LOCUS       DAZKLA010000068        13535 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000068 DAZKLA010000000
VERSION     DAZKLA010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.069"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            160..807
                     /locus_tag="ACG85N_001763"
     CDS             160..807
                     /locus_tag="ACG85N_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0224529.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    936..1152
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1200..3845
                     /locus_tag="ACG85N_001764"
     CDS             1200..3845
                     /locus_tag="ACG85N_001764"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFQ0224530.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4155..5468
                     /locus_tag="ACG85N_001765"
     CDS             4155..5468
                     /locus_tag="ACG85N_001765"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFQ0224531.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5458..6111
                     /locus_tag="ACG85N_001766"
     CDS             5458..6111
                     /locus_tag="ACG85N_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0224532.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6166..6858
                     /gene="radC"
                     /locus_tag="ACG85N_001767"
     CDS             6166..6858
                     /gene="radC"
                     /locus_tag="ACG85N_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFQ0224533.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            7008..7208
                     /locus_tag="ACG85N_001768"
     CDS             7008..7208
                     /locus_tag="ACG85N_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0224534.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7462..9141)
                     /locus_tag="ACG85N_001769"
     CDS             complement(7462..9141)
                     /locus_tag="ACG85N_001769"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0224535.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9143..9355)
                     /locus_tag="ACG85N_001770"
     CDS             complement(9143..9355)
                     /locus_tag="ACG85N_001770"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFQ0224536.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9748..11478
                     /gene="efrA"
                     /locus_tag="ACG85N_001771"
     CDS             9748..11478
                     /gene="efrA"
                     /locus_tag="ACG85N_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFQ0224537.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11475..13235
                     /gene="efrB"
                     /locus_tag="ACG85N_001772"
     CDS             11475..13235
                     /gene="efrB"
                     /locus_tag="ACG85N_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFQ0224538.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13330..13527
                     /locus_tag="ACG85N_001773"
     CDS             13330..13527
                     /locus_tag="ACG85N_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224539.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
BASE COUNT         4288 a         2351 c         2910 g         3986 t
ORIGIN      
        1 cctcatattt tacgtttggt tgagcttatt accgcagatg caagcttttg aacaccgttt
       61 tatttgtctc acaccctttt ccttcctact tgtaaccttg ctcccgtgtt agaaacatgt
      121 tacactaaat atgtgaaatt attaacaagg agcgtcgtta tgcgagaaaa aacagataca
      181 ctgccaaaag caactgcgaa aagattacct ttatatttac gctacttgaa gatgctggat
      241 gactcaggta taagtagaat caaatcaaat gagttcagcg aaatcaccca aattccttct
      301 gcaacgatcc gcagagactt ctcgcaatta ggcgagctag gcagaagcgg atatggctat
      361 gatgtccctt ttcttatcga tgtattcaat aatatcctta atactaaaga agaaaaacga
      421 atcgcattag taggatatgg aaatcttggc aaagcattga aacacaacaa ttttagaaga
      481 aatgaaaatc taaatatcgt gtgcgtcttt gataatgatc cagcactgat caatcgagta
      541 attgatggag aaatgatcta tccgattgat cgtttcgcgg agatagccaa agcaaaaaat
      601 gtgacagtag caatctctac ggtaccaagt aaatattcac aatctgctat tgatgaaatt
      661 gtcaaaggaa atgtaaccgc tatacttaat tttgcgcctg acagagtaac tgtcccagct
      721 tatgtcaatg ttcaatacat tgatttgaca accgagttgc agacgcttat ttactttgat
      781 gagaattact cagaagtttt ttcttaggaa aatttgagta ggcttattga tcttgcgaga
      841 actcgtcgta attctttcaa aaatttttta gtactaaatg aagtggtttt ttcacgacca
      901 cttgttgact aaaaaaaaaa accattgtag aatgaagcat aacgctgaac aagaggagta
      961 actggcacat tcattcttag agagagaact atctgctgaa aggttctcaa tgacaaccag
     1021 tgaaggtagc ttgggagttt ctgtcttgaa tggagtagag atagacgagt cgtgatcgtt
     1081 aactcacttg agaggcaata atgattgatt attgcaattt aggtggtacc gcgtaaatca
     1141 tacgtcctag aatacgaaag tattttagga cgttttttag tttaaaggag gaaaacagca
     1201 tgacggaaga aaataaccta tcaacaaaat ataatccgca agaagttgaa gctggacgtt
     1261 atcagcattg gttagaacaa gatttgttta aaccgagcgg agataaaaaa gcaaaacctt
     1321 attcaatcgt aatcccgccc ccaaatgtta ctggaaaact tcatttaggg catgcttggg
     1381 atacgacctt gcaagatatg ctgattcgtc aaaagagaat gcaaggatat gatactttat
     1441 ggctgcctgg tatggaccat gcaggaatcg cgacacaagc aaaagtagaa gcgaagctta
     1501 gagagcaagg gatttctcgt tatgatctag gtcgtgaaaa gttcatcgat caagtgtggg
     1561 aatggaaaga agagtatgcg agccacatcc gcgaacagtg ggctaaaatg ggcttatctt
     1621 tagattacag tagggaacgt tttacattag atgatggatt atctgaagca gtacgtaaag
     1681 tttttgttac cttatacgaa aaaggtttga tttatcgtgg tgaatatatc atcaactggg
     1741 atcctcaagc acgaacagct ctatctgata tcgaagttat tcataaagat atcgaaggcg
     1801 ccttttatca tatgagctat gaactagcag atggatcagg cgtagtagaa attgcgacaa
     1861 cccgtccaga aacgatgttg ggagatacgg cgattgctgt tcatccagaa gacgagcgtt
     1921 atcaagcgct catcggcaaa aaagtgattc tacctttagt caataaacaa atcccaatca
     1981 ttgcagatga atatgtagat atggaattcg ggacaggtgt agtaaaaatc actccagcac
     2041 atgatccaaa cgactttgag gtaggtaatc gtcatgatct tccacgcgta aatgttatgg
     2101 atgatgatgg tacgatgaat gaactagcag gaaaatatgc tggaatggaa cgttttgctg
     2161 ctcgtaaagc aatcgtgcaa gatctgaaag aaatcggtcg attgattaaa atcgatacga
     2221 tgatccacag tgtgggtcat tccgagcgga caggtgtcgt tgttgaacct cggctgtcta
     2281 ctcaatggtt tgtaaaaatg gcgccgttag cgaaaaaagc cattgataac caagaaacag
     2341 atcaagcagt ggaattcttc cctccaagat tcaatcaaac gtttttacgt tggatggaaa
     2401 atgtccatga ttgggtaatc tcacgacaat tatggtgggg acatcaaatt cctgcatggt
     2461 atcacaaaga aacaggtgaa atgtatgtag ggatggaagc accagcggat agtgaaaact
     2521 gggtacaaga tcctgatgtt ttagacactt ggtttagttc agctttgtgg cctttctcaa
     2581 caatgggctg gccagatacg gaaagcgaag attataagcg atacttccct actagcactt
     2641 tagtaaccgg ttatgacatc attttcttct gggtaagcag aatgattttc caaagcttgg
     2701 aatttactgg cgaacgtcca tttgagaatg tcttgattca tggattgatt cgtgacgaac
     2761 aagggcggaa gatgagtaaa tcattaggta atggaatcga tccgatggaa gtaatcgaaa
     2821 agtatggagc agatgctctc cgctggttct tatcgaatgg ttctgcacca ggacaagatg
     2881 tccgcttcag ctatgaaaaa atggatgctt cttggaactt tatcaacaaa atctggaatg
     2941 cttcacgttt cgttatcatg aatatcgaag gaatgaccgt agaagagatc gatttatctg
     3001 gagaaaaatc agtggctgac cggtggatcc ttactcgttt gaatgaaacg atcgaacgag
     3061 taactgagct atttgatcgt tttgaatttg gtgaagcagg tcgacaactg tataacttta
     3121 tctgggatga tttttgtgac tggtacatcg aaatgagtaa agaagtactt tatggagatg
     3181 atgctgtttc aaaacaaacc acccgtagca tcttagtcca tacacttgat cagatcctac
     3241 gtttattgca tccaatcatg ccattcgtta cagaagaaat atgggaacat attcctcatc
     3301 agggaaattc tctagtagtg gcagaatatc ctgttgtacg tccagagttt gatgatgaaa
     3361 cagcttctaa agggatggaa gtattgaaag aattgattcg agccgttcga aatattcgtt
     3421 cagaagtgaa tacaccattg tctaaaccaa ttacgttgct aattaaaaca aacgatgcta
     3481 aaatcgatca atttttagtt gaaaatacaa gctatatcga acgcttctgt aatcccgaag
     3541 agttgacgat atcaagtgag atcactgcgc cagatctagc gatgtcagct gttctgacag
     3601 gtgcagaaat cttcttgccg ttagcaggat tgatcaatat tgaagaagaa atcaaacgat
     3661 tagaaaaaga actggaaaag tggacaagcg aagtaaaacg tgtccaagga aaactttcta
     3721 atgaacgttt tgtttctaac gcgccggaag aagtagtaga ggcagagcgt gcaaaagaaa
     3781 aagattattt agagaagcaa acggctgtca aagaacgaat cgaaagtttg cgtactattc
     3841 aataagaata gaaaaagaga gtgtgaaatc agctagctat tactaaaagc tagctgattg
     3901 aacatcgtta caggaaagag tttgagcctc actcttagct tcgattcata aggagatgtg
     3961 acataaattt tgtcgcatct cttttttagt ctactcttat tatgtaggag aaggagggag
     4021 gtggatcatg tatcaagagt caataaagta gttgataaaa tcgttcctct ctctgtttct
     4081 tttctctttg tcatactttt atttttctta tgtgttctat ttgttataat aaaagagaaa
     4141 tgggggaatt gttgatgacg attgaagaag caatagaatg gatccatagt cgattaccat
     4201 ttggttcacg tcctggcctt gaccgagtcg aagctttatt agaagtggta gggcatccag
     4261 agagaaaagt aaaaacgatc catatcgctg gaacaaacgg aaaaggttct actgtagctt
     4321 atttgagaag cctattagaa gaggctgggc tgacagtagg tacatttact tcaccttata
     4381 tcgaaacgtt caatgaacgg atcgcaatca atggcaaaag cattccagac aatgatttga
     4441 tccagcttgt aaaaaaatat cagccactga tcaaacaact tgatcaattt gaagcagtag
     4501 ctggtattac tgaattcgag acattaacag caatggcatt tgattatttt ttgatgaaga
     4561 aagtggacgt agcgattatc gaagttggct taggcggctt attggattct accaatgtag
     4621 ctgtaccaat gctgacggga ataacaacga ttggacttga tcataccgaa atacttggag
     4681 atacaattga agaaatcgct gcacaaaaag ctggaatcat caaacaaggc gttccagtag
     4741 taacaggaaa tattgcccca gaagctttta ctgtcatcca acaaacagct aaagcaaaaa
     4801 atgcaccgct ttatgtatac ggggaaaatt ataaaataaa ctatctgcat cctgatgaga
     4861 tgtggggaga agtatttgaa ttttttaatg aaataggaaa gatccctcaa ttaaccactc
     4921 cgcttttagg gcaacatcaa gctgaaaatg caggagtagc catcgaattg tattttgttt
     4981 actgtcaatt ggaaaagctg cctttccgag aaaaagatgt tttgaaggga ttgaagaaat
     5041 ctcgatggcc aggaagaatg gagcgtttga gtaacgagcc attagttgtt ttagatggtg
     5101 cccataatac gcatgcaatg aaccggcttg ttgagaattt gaaaaaagaa ttcaaaggat
     5161 atcaaatcaa cattttgttt tccgccttac gaactaaaaa tgttagagaa atgctagaac
     5221 gattgcagca agtaccaaat gtccatattt atttgacgac ctttgaacat ccaaaagcca
     5281 tcgaattaag tgaagtatca gatttagcaa gtgagagagt atcaatcgtt tcattgtggc
     5341 aattcggatt agcagatata ttggaaaaga tgacaaatga agacgtatta gttgtgaccg
     5401 gttcacttta tttcatttca gaagttcgcc atttattaat acaattagga ggagatcatg
     5461 aagtataatg cagtgatttt tgacatggat ggtttgctgt tcgacacaga gttagtgtat
     5521 tacgaagcca cacaagtagt agcagacaag atgggttttc cttatgataa agacctgtat
     5581 ttaaaattct taggtgtttc agatgaagaa gtatgggcga attatcatat gattttctcg
     5641 gaatttggca aaacaagtgt acaaagattc atagatgatt cataccagga aacgcttgaa
     5701 cgtttttcgc aaggagcggt acaaatgaag cccggtgtgt tggaactcct ctctttttta
     5761 gaagaacatc agattcccaa agttgtcgct tccagtaacc aacgagcagt cattgaactt
     5821 ttattgaaga agaacaatct gtttgaacga tttgagacga ttgtttctgc agaaaatgtt
     5881 caacgagcaa aaccagaccc agaaattttc ttgttagcta atcagaagct aggtacagcg
     5941 aagaaagaaa cattgatttt agaagattca caaaatggta ttttagcagc ttctgcggct
     6001 gagatcccgg tagtcatggt accagatctg cttccaccat cccaagaatt gatcaaaaaa
     6061 acagctgggg ttgtttcttc tttacatgaa atccctgcat tgttaaaata aaaatccatc
     6121 tcttttcgta tcttatttta gataagatag aaaggagatg tttttttggg aaaacgattg
     6181 atcaaagaag tacctactag ctctttacca agggaacgta tggaaattta tggcgcggaa
     6241 gctttatcgg atcaggagct tttggcaatc ttactgcgta ccggtcaaca cccttatagt
     6301 gtgatgtcga ttgctggaaa tctgttaaaa acgtttggag gactggcctc tttacgacag
     6361 gctactttgc atgagttaga agagattcga gggataggac gcgtgaaagc aatcgaaatc
     6421 aaagccttga tcgaattagg tcgacgaatt cagacggatc atgaaagagc ggctccaccg
     6481 gttcgtacca gctacgaatt ggcccagcaa ttgattctgg aaatgaaaga tcacaaacaa
     6541 gaacatttgg tttgtattta tttggacacg aagaatcaag tgatcttaaa aaaaacagtg
     6601 ttcatcggtt cgctgaatca gtcagtcgct catcctagag aaattttcca ttatgctgta
     6661 cgttattgtg cagcaaggat cgttttagcg cataatcatc cttctggtaa tgttattcca
     6721 tcgcagcagg atatgaattt taccaaaagg atacaaaagt gtggagaaat gatggggatc
     6781 actgtactgg accacttgat catcggacgc aaacgatatt ttagtttgag agaagaagga
     6841 atgatggaag agaagtaagt tgctttcttg caaaaaaaat gaagcaagtg tacaattata
     6901 ttgtaatttt gtttgggaat agaagttgaa acatctgttg cacctctatc gtacacgagc
     6961 aatgttacaa tttaaattag tgcttttggc acgaggagga tattattatg gaaaacggaa
     7021 cagtaaaatg gttcaacgct gaaaaaggat ttggatttat ctcacgtgaa gatggaagcg
     7081 acgtattcgt tcacttctca gctatccaag gtgacggatt caaaacatta gaagaaggtc
     7141 aagcagtgac tttcgacgtt gaagaatcag accgcggccc acaagctgca aacgttgtaa
     7201 aagcttaatt ttatctgtaa atcaaattga tggtagacat caatttttac gagagaacaa
     7261 agcgagtaac aaaaatacct gccacaagag acaatttctc ttgtggcagg tatttttttg
     7321 tatgaaaaag taaataaatt taagaggcta tgaaagagcc accttgtatc aaggaataag
     7381 aacaaacaaa aatggcttct catgtttttc atttgttttc tcttatattc aggacaaggt
     7441 gctgatttca taacctctta tttattcttc atctggtgtc attaccattg gcaaaatcat
     7501 tggatgtctt tctgtacgtt cgaataagaa aggctgaagc gcgccagtca tcgcttcttt
     7561 caatttatat tctgaacagt caggtccatt caaacattct cttaaagcgt ggaataagat
     7621 tcgctgagct tcattgatca tttctcctga ttctcgcatg tagacaaatc cacgagagag
     7681 aatatctgga ccagccaaga tttctttttt cttgatatcc actgtcgcga cagctaatac
     7741 aagaccttct tctgaaagca ggtgacggtc acgtaaaacg acattaccaa tatcaccgat
     7801 cccgctacca tcgatataca catctccagc cacaaaatgc ccagcaggac gcgcagaatc
     7861 gctagttaac gctaaaacat cgccattttc catgatgaag cagttttctg caggaacacc
     7921 tacatcttgt gctagagaag catgtacttt caacatgcgg aattctccgt ggacaggaat
     7981 aaaatatttt ggcttcatca aacgaagcat taatttttgt tcttcttgtc caccgtgacc
     8041 ggaagtatgg atgttattga tttttccgtg aatcacttct gcaccagctt cagacagcaa
     8101 attgatcaaa tgattgacac ttgttgtatt accaggaata ggtgaacttg aaaagatcac
     8161 tgtatcgtct ggctggattt ggatttgacg gtgagtacca ttcgcgatgc ggctcaacgc
     8221 ggccatcgct tctccttgag aacctgtaca taggatcatc gtttcattgg caggcaagcg
     8281 attgatttcg tgtgcatcta caaatgtgcc gtctggaact ttgatatatc ctaaacgttg
     8341 accgttgacg atggcatttt ccatgcttcg tccgaaaaca gcgattttac gaccagttgc
     8401 cacagcggca tctgctgctt gctgcaaacg gaagatgtta gaagcaaagc tggcaaaaat
     8461 gatacgtcct tcgatttttg aaaagatctt caaaatagaa tgcccaatcg ttttttctga
     8521 tttagtaaat gttggaatct cagcatttgt actatcagat aaaaggcaca agactccttc
     8581 ttccccgata cgagccattt tatgcaagtt tgctggttcg ccaacaggag tgaaatcgaa
     8641 tttgaagtct ccagtcgcta cgatatttcc agaaggtgtt ttcaccacga tccctaatgc
     8701 atctgggata ctatgggtcg ttctgaaaaa gctgacagaa gtctttctaa aacggatgat
     8761 cgtatcttca ttgatctcat ggagctctgc atctcgaagc aagccatgct catctaattt
     8821 gtttgtgatc aacgctaatg cgagaggacc agcatagata ggaatatttg cttgtttcag
     8881 caagtagggt attccgccga tgtggtcttc gtgcccgtga gtaatcacca aggcttttac
     8941 tttgtgtaag ttttgtatga tgtagctgta gtcgggaatg acatagtcaa ttccaagtaa
     9001 atcgtcttcc gggaatttga tcccggcatc aatcaaaatg atttcatctt ggaattgtac
     9061 cccatacgta tttttcccga tttcacctaa tccaccaata gcaaagacgc ctgtctcgtt
     9121 atttttgatg gaaagtttca tattaaaact ccactaatga gaaatcagca ttttcttttt
     9181 cgtactctag atgacttcct tctagaagtt gaacgaattc aatattgtat ggggtatttt
     9241 cttccacacg ttggcgagcg tctacatcgc tttttgcctc aatgtaaaga gattttgttt
     9301 cttcccgttt agggttgcgt acttttgttt cttgataata aactttatag atcattgttt
     9361 tgtctccttt atctgcgtaa agcagtgatt tagctagttg atttttttag acaacacaaa
     9421 acgaggggac aagccacctg gtttttcata tgctttgact agaatactgg attcgagtca
     9481 aagagacaag cctcgttttt gcttatctgt tcggatattt tttcaaccgt accttctgta
     9541 taaaaaatat acaacaatta attgttattc tagcataaaa cagcaaataa gtatagaaat
     9601 caactagatt gaaagaaaaa atcaatgttt atcatcccgc tataatcata atgacttttt
     9661 tatgagagaa gtgttctatt aataggctcg ttgtgctatc atttttagta atgaaaatgt
     9721 aacaaaagat taaaaggaga tgagaaagtg aaactaatgt ggcattatac catgcgttat
     9781 aagaaattct tgtttttcaa tttcatatgc gtatttggtt ttattttgat tgaattagga
     9841 cttcctacga ttttagcacg tatgatcgat gtaggaatcc gcaatgatga ttttgattat
     9901 gtgaagcaac aagggatatt tatgatcgtt attactgtaa tcggtattat tttgaatatc
     9961 ttgttaggtt attttggttc gaggattacg actaatatcg tagcagatat tcgagatgat
    10021 ttgttcgaaa agatccaaag ttattcgcat caggaatacg aaaatctagg cgtatcttca
    10081 ttgatcactc gtacaacaaa cgatgcttat cagatcatgt tgttcctgca aaatatttta
    10141 cgtatcggtt ttatggcacc aatgatgttc attgtcagtt tgtacatggt catgcgtaca
    10201 agtgtgacac tcagtttgta tgtagtaggt gcattacctt tactattatt ggcagtcgta
    10261 gcgattgcta aggtgtctga accgttatct aaaaagcaac aaaaaaactt agataaaatc
    10321 aacagtatct tgcgggaaaa tctttcagga ttgcgtgtga ttcgtgcatt cgtcaatgaa
    10381 aagtttgaag aaaaacgttt tgctgtagtc aatgagaatt atgcttctag ttcaaaaagc
    10441 ttattccgtt tgatggctgt tgcacaaccg ggtttttatt tcttatttaa tatcgtaatg
    10501 gtattgatca tctggagcgg ggcagtgcaa atcgatgcag gcgagcttcg tgtaggagat
    10561 ttgattgcgt ttatcgagta tatattccat gccttgttct cattcatgtt atttgctagt
    10621 gtgttcatga tgtatccaag agcagctgtt tcagctggac gtatccaaga agccctagat
    10681 gccaaacctt cgattgtaga agatccaaat ggtatatctg atacaaaaac taaagggtat
    10741 atcgaatttc aaaatgtaac atttgcttat ccaggccatt cgcaagagcc agtgatacga
    10801 aatgtcagtt ttacagcctc tccaggagag accgttgctt ttattggaag taccggtagc
    10861 ggaaaatcta cgttgatcca attgatccct cgcttttatg atgtgagtga aggaaagatc
    10921 ttgatcgatg gagtagacgt gagagattat cggctaagca agttgcgaga taagattggg
    10981 tttattccgc aaaaagcgct gctatttact ggaacgattg cagataattt gcgatacggg
    11041 aaagaagatg ctacgcaaga agaaatggaa cgtgcagctg agattgctca agcttcagaa
    11101 ttcatttctc gaaaaccaga tggatttaat gaacatttat cagaaggtgg ttccaatttt
    11161 tcaggtggac aaaaacaacg attggcaata gccagagcca tcatacgccg tccggaaatc
    11221 tatatttttg atgacagctt ttctgcatta gattatcaaa cggatgcgaa gttacgtgct
    11281 cgtctgaaaa aagaaacgac agaatctact gtattgatcg tagcacaacg tgtgggaacg
    11341 atcatgcatg cagataagat catcgtatta aatgaaggag aagtcgtcgg tatggggacg
    11401 catcgggagt tgttggaaaa atgcccgatt tattatgata ttgccgcttc tcaattatca
    11461 aaggaggaat tagcatgaaa aaaacctttt cttccttaaa acgtttagct cgatatatca
    11521 gaccgtatcg tgagactttt cttttagtga tcctatttac agttctgaca gtggctttca
    11581 acgtggcttt gccatatgtt acaggacttc ctactacaga aatcagccgt aatattgcca
    11641 atggcgaatc aatcaatttt tcttatatat tcaaatgtct catttggatc acagttgttg
    11701 gaatagggta ttgtatttcg caattacttt ctggtgtttt gatgacaaat gtcgttcaaa
    11761 gtgccatgca tgatttgaga agagacatag aagaaaaaat caatcgtctt ccggtttcat
    11821 atttcgataa aaaccagcaa ggaaatattt tgtctcgtgt gacgaatgat gtagatgcag
    11881 tcagcggtgc tttacaacaa gcttttattg gtatcgtaaa tgcgatattg ggaattacgt
    11941 tggctgcagc aatgatgttt tatatccagc cgatgatggc gctgatctct atgatcatga
    12001 ttccaacatc gatctggatc tcaaaacgag tgatcaatgc ttcgcagaaa tattttcaaa
    12061 gcatgcagaa ttctcttggt gagctaaacg gctacgtcca agaaaatatg acaggtttta
    12121 gtgtattgaa agtatatgga cgcgaaaaag aaacattgga aggattcaaa aaagttaatc
    12181 acagcttgaa atactttggg tttcgtgcag cattcatttc tggcttgatg atgcctctag
    12241 ttcaattaac tgcctatgct acttatattg gtatggctgt tctaggaagc ttttatgtta
    12301 ttactggggc gatcgttgtc ggtcaactac aagcattcat ccaatatatt tggcaaatca
    12361 gtcagccaat ggggaacgtg acacaattgt cttcagtttt gcaaagtgca tcagcggcaa
    12421 cgaaacgtgt attcgagatt ttagatgagc cagaagaaaa agaaaacaaa gtagatgctt
    12481 cactgccaga aacgatttta ggagatgtga cattcgatca tgtcgatttt gcatatgatc
    12541 cgaagaaacc attaatccaa gatttgaatt ttgaagtgaa agctggtcaa accgtagctg
    12601 tggtcggtcc gaccggtgcc ggcaagacga cattgatcaa tctattgatg cgtttttatg
    12661 acgtagacaa aggagcaata aggattgatg gaatcgacac caaatcaatg agtcgcagtg
    12721 atgtgcgttc gctatttggt atggtcttgc aagatgcatg gctttatcaa ggaacgattg
    12781 cagataatat ccgcttcgga aagcttgatg cgacagatta tgaagtagtt gatgctgcga
    12841 aaacagcaaa cgtagatcat tttatccgaa cgatgccaga tggatatgat atggccatca
    12901 attctgaggg agacaatgtg tctttaggac aaaaacagtt gctgacgatt gcccgagcag
    12961 tggtctccga tcctaaaatc ttgattttag atgaagcaac aagttcagta gacacccgtt
    13021 tagaagcgtt gattcaaaaa gcaatggatc gcgtgatgga gggacggaca agtttcgtca
    13081 tcgctcaccg tttatcgaca atccgtgatg cagatctgat tctagtgatg gatcaaggtc
    13141 agatcattga aaaaggaacg catgagagtt tattggcaca aggtggattt tatgaaaaac
    13201 tgtataacag ccaatttgct gaggaagcag aataagtcat ataagaataa aacagtttaa
    13261 aacttatttt ttgttctcta ttcttgataa tggtaaagta aagttatcaa gaaaagtgag
    13321 gacacggaga tggaacagtt tttaaatgaa aaaattactg aagccaaaag agaagttgat
    13381 cgtttaacgc acgtctgtca gacgaagcct tttgattcag ctgatttttt tgaaaatgag
    13441 tttcaaatcg aacgcttggc agcacaaata gaagtgtacg aattaatttt aaaaaaagta
    13501 atagaagaaa atatagatgt acaataaaaa caggt
//
LOCUS       DAZKLA010000069        13429 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000069 DAZKLA010000000
VERSION     DAZKLA010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.070"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACG85N_001774"
     CDS             complement(<1..914)
                     /locus_tag="ACG85N_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFQ0224540.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACG85N_001775"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACG85N_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFQ0224541.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="ACG85N_001776"
     CDS             2006..2176
                     /locus_tag="ACG85N_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224542.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="ACG85N_001777"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="ACG85N_001777"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFQ0224543.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="ACG85N_001778"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="ACG85N_001778"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFQ0224544.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /gene="dapA"
                     /locus_tag="ACG85N_001779"
     CDS             4440..5321
                     /gene="dapA"
                     /locus_tag="ACG85N_001779"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFQ0224545.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /gene="dapB"
                     /locus_tag="ACG85N_001780"
     CDS             5318..6034
                     /gene="dapB"
                     /locus_tag="ACG85N_001780"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFQ0224546.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="ACG85N_001781"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="ACG85N_001781"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFQ0224547.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="ACG85N_001782"
     CDS             6751..7752
                     /locus_tag="ACG85N_001782"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFQ0224548.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="ACG85N_001783"
     CDS             7749..9110
                     /locus_tag="ACG85N_001783"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFQ0224549.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="ACG85N_001784"
     CDS             9070..10221
                     /locus_tag="ACG85N_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0224550.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="ACG85N_001785"
     CDS             10196..11365
                     /locus_tag="ACG85N_001785"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0224551.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="ACG85N_001786"
     CDS             11540..12919
                     /locus_tag="ACG85N_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0224552.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="ACG85N_001787"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="ACG85N_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4484 a         2339 c         2924 g         3682 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DAZKLA010000070        13299 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000070 DAZKLA010000000
VERSION     DAZKLA010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.071"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(188..2263)
                     /gene="glyS"
                     /locus_tag="ACG85N_001788"
     CDS             complement(188..2263)
                     /gene="glyS"
                     /locus_tag="ACG85N_001788"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFQ0224553.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2265..3182)
                     /gene="glyQ"
                     /locus_tag="ACG85N_001789"
     CDS             complement(2265..3182)
                     /gene="glyQ"
                     /locus_tag="ACG85N_001789"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0224554.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3568..4386)
                     /gene="recO"
                     /locus_tag="ACG85N_001790"
     CDS             complement(3568..4386)
                     /gene="recO"
                     /locus_tag="ACG85N_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFQ0224555.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(4529..5428)
                     /gene="era"
                     /locus_tag="ACG85N_001791"
     CDS             complement(4529..5428)
                     /gene="era"
                     /locus_tag="ACG85N_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFQ0224556.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5443..5838)
                     /locus_tag="ACG85N_001792"
     CDS             complement(5443..5838)
                     /locus_tag="ACG85N_001792"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0224557.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(5822..6298)
                     /gene="ybeY"
                     /locus_tag="ACG85N_001793"
     CDS             complement(5822..6298)
                     /gene="ybeY"
                     /locus_tag="ACG85N_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFQ0224558.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6327..8519)
                     /locus_tag="ACG85N_001794"
     CDS             complement(6327..8519)
                     /locus_tag="ACG85N_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFQ0224559.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8543..9514)
                     /locus_tag="ACG85N_001795"
     CDS             complement(8543..9514)
                     /locus_tag="ACG85N_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFQ0224560.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10219..11598
                     /gene="brnQ"
                     /locus_tag="ACG85N_001796"
     CDS             10219..11598
                     /gene="brnQ"
                     /locus_tag="ACG85N_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0224561.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            11880..13220
                     /locus_tag="ACG85N_001797"
     CDS             11880..13220
                     /locus_tag="ACG85N_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0224562.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTILRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPLSMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKRDRRS
                     KHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVDRLLSYDDSLKRGYEVYQDF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT         3681 a         2842 c         2444 g         4332 t
ORIGIN      
        1 aacagtaaca agattataat taatcgtttt catttttatt tcctcacttt tcatgagcgt
       61 tttgaaaatt cgaacaaaga ataaaataac aaaaattatt aaaatagaat taataacagc
      121 tatttataac aaaaaaaaca gagacacagc acgtttcgtg catgctctct gtttttcaaa
      181 caaaagacta ttttgtgatg atttgatcca agcttgctaa tgacaaagcc atggcagcta
      241 tttgacttag ttgtgttagt cggttcttac gtactgctgg ttcatcagcc atgaccatcg
      301 tttgatcaaa ataattttcg atcagtggac gaagagcgac aagctggtcg tagttttcgt
      361 ggatcgtttg gtcagcaaaa cttgctttca ttgcttctac tgctttgtgt aattctttct
      421 ctgcgtcgtt ttcgaacagc tcagggtcta cttctgattg tttttcaagc tgcgcttttt
      481 tcgctaagtt cacgacacgt gtcaaagctt ccattgaagg tttgaagtct tcatctgcaa
      541 gatgttgttt gaacaattgt gctgctgcaa atacttttgt cagatctttt tgttccgcat
      601 tcaaaacagc atcgatgaca tcatgacgaa tattcttcgt tgtcaataat tggcgcagac
      661 ggcctttgat aaagtcgatc acttgttgtt gcccttctga taaaccaatg ccgaaacgat
      721 ccgtatcttt actgataaca tcgctaatcg tttcttgcag aacagacaaa ggaaaggccc
      781 attttttcga ttcgatgatc cgaacgacac cgtatgcttg gcgacgtaag gcataaggat
      841 cgtttgaccc gcttggaatc atcccaactg caaagaaaga aaagatgcta tctaatttat
      901 ctgcaatcgc taaaacagaa cctactgtag actgaggtag ctctccttca ctggatatag
      961 gcatgtaatg ctcacgaatc gctgtcgcta cagcaggttt ctcgcctttc agaagagcat
     1021 atttttcgcc catgatccct tgtagttcag gaaattcccc taccatgttg gttactagat
     1081 caaatttata gatttcagca gctcgttgca aatccatcaa ttcttcctca gatagtccta
     1141 cttgttcgcc aatgatacgg gcaatgactt gtacccgttg cattttctca tagatactac
     1201 cgattttttc atggaaagta acagttttta gtttttcaac gtattggtcg attgaagctt
     1261 tcttgtcttc attataaaag aattccgcat cttctaaacg agccgtcaat actttttgat
     1321 tgcctcgaac cacatcttcc aattttaccc gatcaccatt acgagcagaa ataaaatgag
     1381 gaagcagcat accttgatca ttccgtacct cgaaataacg ttgatgttct ttcatagaag
     1441 tgactagtac ttcttcagga acagaaagat atttttcttc gaagtcgcca gaaaagacag
     1501 ttggatattc gactaaattg ttgacttctt ctagtaaatc ttcgtctaga tcaataaccc
     1561 agtgattttt ttctgctaat tcgtttgctt gtgtaacgat catttcttgt cttttttgcg
     1621 gagacgcaat gacgaattgt tcattcaatt tttcttcata ttcttttgca tgcgcaaatg
     1681 tcacatcttc gcctaagaaa cgatgacctc gagaggctct gcctgttttg acatccaaga
     1741 tttcgaatgg gattacttcg tcatctaaca atgccacgat ccagtgaata gggcggatat
     1801 attcaaaatc gtattctgcc caatgcatcg ttacagggaa agtcaaactt ttgatcaccc
     1861 ctgctaagcc tgtcaatact tcttttgctt ccttaccatg agtgtatttc gttacataga
     1921 catattccac accttttaat tctctaaaag tgatagcgtc tgtcgtcaga ccttgtccgc
     1981 ggacaaatcc ttccgctgct ttcgtccagt ttccatctgc gtccaaagca atttttttgg
     2041 ctggtccttt gacttcttct tcactgtccg cttgtcgttc tggaatattt gttactttga
     2101 ttgctaaacg gcgcggtgtg gaaaatgttt cgatcgtatc ataagtcaat tggttttcat
     2161 ctaaaaattt tgctgttttt tcttctaatt gtttcatgct tggtgtgaca acatgggcag
     2221 gcatttcttc cagcccgatt tcaagtaata agtcttttgc catcttattc agcctccttc
     2281 acggtttctg gatttgctgt gtttttattc aacaaaggga atcctaattt ttcgcgttct
     2341 gccacaaaga ttttggctac agcacgagcc atgttgcgga tgcgagaaag ataaccggca
     2401 cgctctgtta cggatactgc accgcgggcg tctaataagt tgaatgtatg gctacatttc
     2461 aagatataat catacgccgg atgaacaaga ttttcatcga tacagcgttt agcttctttt
     2521 tcaaaacgat caaagttgct tagtagcaag tcttggtcgc ttacttcaaa agaatatttg
     2581 gaatgttcat attctggttg tttgaagatc tcgccatatt tcacgccatc agcccattct
     2641 aaatcataga cactttcaac ttcttggata taagaagcaa gacgttctaa tccataagtg
     2701 atctcagaag ttactggttt acaaggcagt ccaccaactt gctggaagta cgtgaattgt
     2761 gtgatttcca ttccgtcaag ccatacttcc caacccacac cggcacagcc catggatggg
     2821 ttctcccagt tgtcttcaac aaaacggatg tcgtgttcta atggatcgat tcctaaaagg
     2881 cgcaagcttt ctaaataaag ctcttgaata ttctcaggtg aaggtttcat caccacttgg
     2941 aattgatgat gctgatacaa acggtttggg ttttcaccat aacgtccatc agctggtcgg
     3001 cgagatggct ctacgtaagc agcattccat ggttctggtc cgattgcccg taaaaaggta
     3061 tatgggctca tcgtacctgc tcctttttct gtgtcataag cttgcatcag catgcagccg
     3121 tttgatgacc aaaatttttg taatgttaaa atcatatctt ggacggtcaa ttttttactc
     3181 atctgttttg cccctttcaa ttttaaagcg ttgctgtcaa ataagaatac aggaaagaaa
     3241 acgacaaaaa atccctatgt ctgattacag acatagggac gaataattcg cggttccacc
     3301 ctacttccga ataaagattc ggcagcttca tttatcgtac tcgcaagtgc ctcttcaaaa
     3361 atcgtttggg tccggctccc actctcccga actcgctaaa ccacgtgttt tttactgatc
     3421 ttggtcaatg tacgtattca ttttgcagtt ttcttgtttc tataatagat cctatcactg
     3481 aacgatctat ttaatctatg gttaataata gacggaactc cctgtgtttg tcaaatggtt
     3541 ttgatttttt gttgtatgtt ttatttacta ttcttctttt tcaggtgtct ctttttcttt
     3601 ttcagacatg tctttctctg acgtctttaa tacgtgtgcc caagacttca tttgattgat
     3661 gaactttttg cttttcaaat ggataccgac atattcatca tacagctggt cgatgagttc
     3721 acggattgct tgtttggttt cttctttcag atggattcca ctaattttat cataagaaat
     3781 agctgaaaac atccgaacga aataaacagc tcgaggatct gcatgataac ggtgatcgtc
     3841 catcgaccaa tgtctttcgc atatcacacc accatatttg gaagaatagt caaattttcc
     3901 ttttgtttcc ccgcatacga cgcagtgtgt ccagattggt gcaattccga agcgttgcag
     3961 tatttggact tcaaaaatat tcgtgatgat ttctgcatcg tctccttggt ccatccgttg
     4021 cagtgcttgt tgcgtaaact ggaacaaatg cgggtcgtac tgatgatctt cgattgccgc
     4081 atctactaga ttcaataaat atgtgccata gccgctcaaa aagatgtctt cttggatgtg
     4141 tcgaaacgca tggacatctt tgacactgct taaaaatgac aaaccttcac ttcgaaaatt
     4201 cccgatataa actgcttcag taaaagggag caaagccgga gctaagggct gattttgccg
     4261 atggatctct ttgatataaa acatcatttt gccagaagat tctgtaaaga ttttgactaa
     4321 tttatctttt tctttgtaat tacggctaga taaaatcaag ccttttgatt ctgcaagctg
     4381 gctcatattc tccctcctaa aaaataaatc tggaaaagca gattctgccg atccagatca
     4441 tttatgttgc gatcttccgc agcttttctc aacacgcgga tttttattca gtttatttct
     4501 atacctaatt tacaagaagc tcagaatatc agtattcgtc ttttcggtag ccgaagtctt
     4561 gcagatagac ttttttgtct cgccagtctt tttgaacttt gacccaaagt tctagaaaga
     4621 ctttgtctcc taaaagattt tcgatatctt ttcttgcttt cgttcctact tgcttcagca
     4681 tttttccgcc tttgccgata atgatccctt tttggctatc tctttcgaca ataatcgtag
     4741 cctggatatg gattttatca ttctcgtttc gcttcatcga atcaacaacg actgcgacag
     4801 aatgaggcac ttcgtctcgt gttaaaaaca agactttctc acggatcaat tcagaaacga
     4861 tgaagtattc aggatgatca gtcacttgat catctggaaa atattgtggg ccctctggca
     4921 tttcactgac taatacatcc atcaaacgtt cgacgttgtt tccttctgta gcagagattg
     4981 gaatcacttg tgcaaaaggc atctgcgttg aataatcttc gatgattccc aacaactcgt
     5041 ctggatggat tttgtcgatt ttattgataa tcagataaac aggagaatgc acgtttttta
     5101 gacgttcaat aataaaatcg tctccccgac cgcgtttttg atcagcacta atcataaata
     5161 agaccgcgtc cacttctttc aacgcactgt aagctgcttc taccataaaa tctcctaaac
     5221 gatgctttgg tttatggatt cctggtgtat caataaaaat caactgagct tctggtgtag
     5281 tgtatactcc ttggatttta tttctagttg tctgtgcctt gtcactcatg atggcaattt
     5341 tttgacccac gattcgattc aataatgtgg atttccctac atttgggcgg ccgacgattg
     5401 cgacaaaccc tgatttatgt tctggcatat atgttctcct ttttaattaa acaacaaatg
     5461 atatatttta gggacaaata aaatcaaccc aacgatgacg gtaaaacaag cggtcactaa
     5521 aactgctcca gctgccatat ctttgatttt tttcccaatc ggatgaaaat gaaaatccgt
     5581 aaacatgtcc acgacatttt caaaaacagt attgatgatc tctactatcc atacgagaaa
     5641 aacagctaaa agcagccata accattctat ccgatcaagt tggaaaatca cgcccataaa
     5701 caaagcgagg attccgaacg caacatgttt cctcatgttg cgttcttcct caaaaacagt
     5761 tttgactcct tggacggcaa attccacgga catcaggaaa tgtttgtttt tctctacgtt
     5821 tttatctttt aagtccatag gcatctaaga tctctttctg taaaccaaac atttctttct
     5881 catcctctgg tgtcatatgg tcatacccgt tgatatgcaa gaatccatga accgctaaaa
     5941 agccaagttc ccgatcatac gaatggccgt aagcttctgc ttgctctgca gcacgttcag
     6001 tagagatcat gatgtctccc agtacacgag gcaatggatt ctctccatct tcaaaaatca
     6061 ctgggatttc gtcttcgccc tcttcttcca aggcaaaact gatgacgtct gtcggtgcat
     6121 cttttccacg gtaatcacga ttgattactt ggattgctgc attgtccatg aaagtaacag
     6181 acatttctgt ttcttctggc aactctaaat atgatgctgc aaattgcagc aagtcgtcga
     6241 tttctttcat tttctcttct gagacttggt ttgtttcatc catgaaggta atatccatta
     6301 tacatcgctt ctttctattc ttcttgctat tcttcttgtt cttcttttat cttctcaact
     6361 tccgatttca ttttaggata tttgatccga gaatggaagg tactgcttaa accgctgatc
     6421 aatgattttt ctactttacg gatttctttt aacgtcaatc cactgtcgtc caattgtcca
     6481 tcggaaatac gctcttcaat aagattatgg acaaaacttt cgattttttc gctcgttgga
     6541 tggtccattg cccgtactgc cgcttcacaa ctgtcagcta tattgacgat tcctgcttca
     6601 cggctttgag gttttggtcc aggataacgg aattgctctt ccgtaacttc cgggttgcgt
     6661 tcctttgcct taacgtagaa ataacgcatc agcgtggttc catgatgctg tctacaaata
     6721 tcgatcacca tttgcggcat attataatct tccaatattt tggcaccatc tgtcacatgt
     6781 ccaaagataa tatgtttact gtcttctggc aataagaaat tgtgcggatt ttccgctcct
     6841 gaaggcaagt tctcaacgaa aaagctggcg tgtttgatct tgccgatatc atgataatag
     6901 caagctactc gagttagtaa agaacgtcca ccgatttctg ctacagcatt agcacttaga
     6961 ttggctacca tcatgctatg gtgatacgta cctggtgctt cttccagcaa ctgtttcaat
     7021 aaaggatgat tgggattgct gagttcattt aatacgatca cactatcatc attgaccatc
     7081 aattcaatat aggggtgcag acctatcgtc agcaagaatg aaaacagtgc acctgtgaat
     7141 ccgcagacta gcgttaacca agtcgttgca tccccaaagc tcattccttg gtagataatc
     7201 aaaatgacat ccatcatcac aggaaaaata atgacccaca tagcagcgga taacccttgt
     7261 tctgagatcc gtttgcgtct gacgatcgtt gccatgagac cagaaaatac ataggacatc
     7321 aaaataactg tcagaaagtt cgtgccaatc gaatcataaa aaatgaaaag tgcggatact
     7381 gcttgaaaaa taccagccat gatccctgct cttcgattca agaaaaagct taaaacaagt
     7441 ggagcaaaag ccgcggggta aaacagcgga atataagcag cttgttctgt ttggaagagt
     7501 tggaaaaatt tcattagtac gacgcttact gacattgccg tcacgtagaa aagtacgtat
     7561 tttgttcttt ctcctgcatt ttggaattga agtgaattat aaatcagtac accgatttga
     7621 agcaaaacag ctaaaaccat ggcaatgagt gggaaaatag aagtcgtttg attcgtcaat
     7681 cctagtaatt ccaatttttt catcgctgaa gcatcaattt gatttccttc gcgaacaata
     7741 atttctcctt ggaagatcat cactggttgt acagcttctc gtgcagattg ttttaattct
     7801 tctgtctttt tctcattcaa tacatcatta actacgatgc cttgatccac taagtagcga
     7861 atcatttgtt gctgctctgt tgtgacattc aaatattgaa tctgatcttc tgcttcttgt
     7921 ttaaaagctt ccaattcgct ttcacgaact tgtttactca tctgttcgtc aatgagcttc
     7981 aaactttcat ccctaactgt gtgaatttcc gtttcagtca tttcaaatat cgtcgaataa
     8041 aaattgttcg gcagtttttg gtaaaacgca acgttttctg cattcacgtt ttcaaaattt
     8101 ttcttcagtg tagcaatccg ctcttctgta gtaggtttcg ggatattttc atcttccttc
     8161 gccttatcta ccttgccatt ataagatttg ttgatggtat cattcgtctt atcgatcaat
     8221 tcgaataatt gcttgatccg attgttctgg tcatctgcca gatctttttg ataagtatat
     8281 tctggagtca ctgcttctgc tgccagtttt cttctttgtt cggtttcttc cgtgttctca
     8341 atcgttttat tcgcacggat actctcttct gctacttggc cttctctgaa atcaatggcc
     8401 ttttgtttga cactactaaa tgtaacgccg cagacaatga taaaaaacaa gaacagaagg
     8461 aaaggcaatg ctgcctttcc gagtttttct tgtagtttct gcagtgtttt attcaacatg
     8521 tgcttatcct cctaagcaaa gcttattctt gatgaagatc cgccttttca taagctttga
     8581 tgatttcggc aactacaggg tgccgaacaa cgtcacttgc ttcaaaatta acaaaatcga
     8641 tttttttgat ttgctttaat gttcgttctg catgaacaag accacttgtc gtcccacgag
     8701 gcaagtctat ctgacttgta tccccattga caatcatttt tgattgataa ccgagacggg
     8761 tcaagaacat tttcatctga gcgactgtcg tgttttgtgc ttcgtccaag ataacgaatg
     8821 catcatccaa tgtccgcccc cgcatgtaag ctaaaggtgc gatttcgatc acgcctcgtt
     8881 ccatcaatcg gttcgtatga tccaatccaa aaatttggta taaagcatca taaacaggcc
     8941 gcagataagg atccactttt tctttcagat ccccgggtag aaagcctagg ttttcccctg
     9001 cctccacagc gggccgcgtc aaaatgattt tttgcacttc gccttttttt agggcagcaa
     9061 ttgctaatac tacggcaaga aacgttttcc ctgttccggc tggccctaca ccaaatacga
     9121 tgtcatgttt ggctaccgat tcaacgtatt tcttttgacc gctgtttttt acccgtatag
     9181 gttttccatt acggtctttg acgatctctt cctcatacat ctcaacaaaa taatctaatg
     9241 tacctttttg ccccattttc aatgccgtca caacatctgg cgtacttacg tggattcctc
     9301 gtttgatcaa ttcctgtaat gtccgaatga ctgaggcagc caaggaaatc tgggattgct
     9361 caccaatgat ctgaatgatt tctccacgtg tatggatggt tgtttccgtt gtttcctcga
     9421 tcagttttat atgcttgtcg tgtgcaccta aaagcatgct gacatcatca gcagctgtca
     9481 atctaatctc taaagaacta gactcttccg tcaaaaatag acatcccctt tttttcgatt
     9541 cattctttcc tattttacca tactcagaag aaactatata gcaaataaga aaaggttgta
     9601 aaagagtcgc ttaaccatac gtaccaagaa acgttaaaaa atagccgttt ccattcgcta
     9661 aaggtctcgt tttgataaaa agaagctgag aatgtaagtt atatgaaagt ggagaacaaa
     9721 atatttcgtt tcattcaaca agtattctct aggtattttt cctgtttcaa aaaaagaaaa
     9781 caaaatagtt aaaatattct tgcatttcaa aaaaaacagg tgtattatta cgtcaacaaa
     9841 taaacataca gcaaagcgtc gaggaaaaca gtaggctatt caacgattta ttcccagtga
     9901 gtcatgtaag tgagaaatga caataaatcg aatagacgaa catcatttcc gagtcaatgt
     9961 cgaaaacgta gatcattgtg gctgcctcca ttatcaggct agcggataac tttatcaaaa
    10021 agcgtccgtg aatgaagaag cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta
    10081 gagccctttt cccaagtgga aaagggcttt tttttatttt aaggtggtgg ttttcatgga
    10141 atctgatatt agtggcatta ctaattgctg caacgtttat atcgtcaatc aaaatgataa
    10201 ttattggagg aaaaatttat ggaagaaaca aaaacttatt catggaaaca actattgatg
    10261 gtcagttcac ttatcttcgg gatgttcttc gggtcaggta atctaatctt tcctgtccac
    10321 ttaggtcaaa tggctggcgc aaactgggtc agtgctggcg taggatttgc tatctcaggt
    10381 gctcttttcc cattactagc tatcttagct gtagtagtga caaaaagtga tggtctgtat
    10441 gacttagcta aacctgtcgg aaaatggtat gctgccttgt tcttaatcct tgttcatttg
    10501 acgatcggac catttttcgg tacaccacgg acagctgcta ctgcttttga aatggctgct
    10561 aaaccatttt tgcctgaaaa atataattca ttaggtatgt ttttattctc tgctcttttc
    10621 tttttacttg cttatttcct tactgtccat ccaacacggt tggtcaaata cgtcggaaag
    10681 tatctaaact ccttgtttct tgcattatta gcaatcattt ttgtcgtttc tttattccat
    10741 ccaatgggaa atctaaatca atctgctgca gatacttatc aaacaaacgc tttaacaaat
    10801 ggtgtcctag aaggttataa cacggttgat gcagtagctt tgcttgcctt aagtgtcact
    10861 tttgtccatg cagtccgtga ccttggtttc aaagataaaa aagtcacaga attaacggca
    10921 aaagctggta gcctgagcgt tgcgctagaa gtcatgatct attttggact tgttcttctc
    10981 ggtgcgatga gtttgaataa gatgtcttta tcagaaaatg gcggaactgc actttctcag
    11041 atcactaatt tttatttagg taacttcggc gctagcttct taggagtgat ggtcacatta
    11101 ggtgtcttca ctaccgctat gggactcgtt gtttcttttg cgcaagattt ccataaactg
    11161 ttccctaaag tcagttatat gacatggctg cggttaacga ctttcgtctc ttttgtcgtt
    11221 gcaaatgctg gtttagacaa tatcatccaa tggtctttac cagtattgat gcttttgtat
    11281 ccattgtcgc ttgcgttgat cttgctttcc ttaacagcaa aatttttcca aaagacacca
    11341 tttgtctatc aagtaacgat gttgtttgct gctgttcctg ccgtattgga tatgctggca
    11401 aattcgcctg ctttagtgag tcaacaaaga gttgtagcaa gcatgctcga attttatcat
    11461 catcacgttc cttttgctgc attaggactt ggctggatgg tcccaacgtt acttggttac
    11521 gctggcagcc tactgtttta ttacgcctat cgcctatcag ggtataaaca agaagcaaat
    11581 gaactaccag aagaataacc aaatcaaaca caatttaaca taagaaatat taaacaggaa
    11641 aaaaccagct agatgaaaga atcctattct ttcgtctagc tattttttag ttctcttttt
    11701 tacgcgcaca aaaaaactgc ggacaatgat tgtccgcagt ttaaatgggc tctataatat
    11761 ctagtgttgg tgacgccttc tgttgccaat actttttctt ttttctaata aacgcaaaca
    11821 gacaccttga aatcctttag aataaagtcg acgaaaacca ttcaaaggag tgtttcaaga
    11881 tgtcctattc agagtttacc aaagagttac tagatattct agacctaaat cttatctttc
    11941 atgaagatgc ctttcgcaaa gagcgaatca atgacgaaac atgttttgtt tttgatggca
    12001 cattgaccta tcaaccagag gaatgcttcc attgtcacta tcaaaataag caaacgatca
    12061 ttaaatgggg ctggaaaaaa gtatccatct tattgaatga tgtcagtaac tacaagacca
    12121 ttctgcggat taacaaacag cgcttcaaat gtaaacactg tggaaagact tttttagcag
    12181 aagattcagt tagtgatcgt cgctgctcca ttgctcgcag agtcaaacaa gccattcttg
    12241 agctattaag cgaacctctt tctatgtctt tgatcgcacg tatgaagcac atttctccga
    12301 ccactgtcat tcgaatactt cgcagccttc ggcctaaaac cgtttctttg aaccagccgt
    12361 taccagaagt ggtgtgtttc gatgaattca aatcggtgaa aaacgtctcg ggagccatga
    12421 gctttgtcat gatggacggc aagacccatc aactgattga tattgtggaa aatcggcaac
    12481 taaatccttt acgcgactat ttcttgcgct accctagaaa agtccgtgaa caagtacgtc
    12541 tagtggtttc agatttctat acaccctatc gcacattggt gaaagagtgc tttccgaatg
    12601 cccgaatcgt cgccgatcgc tttcatatta gccaacacat cggtcgggcc tttaccaacc
    12661 accgaattca agtaatgaag acctttaaaa aaagggatcg tcggtcgaag cacttaaaga
    12721 aatactggag actacttcaa aagaatgcgt gggaattgaa aggtcagcat cgctactggc
    12781 gtccgtcatt tcgagatcat ctgacagaag cagagatagt cgatcggcta ttatcttatg
    12841 atgactcctt aaagcgaggc tacgaagtct accaggattt tctttccgct atcagaagac
    12901 aagatgttcc tgaattcgtc gcgctcctaa aagaagacta caaggagttg ccagagcact
    12961 accaaccagt gttcacgacc ttcaagaaat atcgaacaga aatcaaacga gcgttgcgag
    13021 ttccctattc caacggccct atagaatgtt tgaacaacca tatcaaagta ctaaaacgaa
    13081 ttgcctatgg ctttagaaac tttcaaaact accaagaacg aatcttttta tatcgaggaa
    13141 aatattttaa gaaaactaag aacactaccc aactgactaa agctagaaca acaacacggc
    13201 tagacaagat agctgtctag ccgtgtgagt atctcgatta ttctattgaa tttcggctct
    13261 atactttttg atgttggtga acttcaccaa catcttttt
//
LOCUS       DAZKLA010000071        13191 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000071 DAZKLA010000000
VERSION     DAZKLA010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.072"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..1897
                     /gene="yfcC"
                     /locus_tag="ACG85N_001798"
     CDS             158..1897
                     /gene="yfcC"
                     /locus_tag="ACG85N_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFQ0224563.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2054..3238
                     /gene="iadA"
                     /locus_tag="ACG85N_001799"
     CDS             2054..3238
                     /gene="iadA"
                     /locus_tag="ACG85N_001799"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFQ0224564.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3349..3552
                     /locus_tag="ACG85N_001800"
     CDS             3349..3552
                     /locus_tag="ACG85N_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0224565.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3653..4315)
                     /locus_tag="ACG85N_001801"
     CDS             complement(3653..4315)
                     /locus_tag="ACG85N_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224566.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4328..5830)
                     /locus_tag="ACG85N_001802"
     CDS             complement(4328..5830)
                     /locus_tag="ACG85N_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0224567.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESRETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6378..8057
                     /locus_tag="ACG85N_001803"
     CDS             6378..8057
                     /locus_tag="ACG85N_001803"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0224568.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8184..8663
                     /locus_tag="ACG85N_001804"
     CDS             8184..8663
                     /locus_tag="ACG85N_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0224569.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8882..9562
                     /locus_tag="ACG85N_001805"
     CDS             8882..9562
                     /locus_tag="ACG85N_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFQ0224570.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9570..10901
                     /locus_tag="ACG85N_001806"
     CDS             9570..10901
                     /locus_tag="ACG85N_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFQ0224571.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10916..11878
                     /locus_tag="ACG85N_001807"
     CDS             10916..11878
                     /locus_tag="ACG85N_001807"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFQ0224572.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11989..12327
                     /locus_tag="ACG85N_001808"
     CDS             11989..12327
                     /locus_tag="ACG85N_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFQ0224573.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12406..13113
                     /locus_tag="ACG85N_001809"
     CDS             12406..13113
                     /locus_tag="ACG85N_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFQ0224574.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4208 a         2324 c         2886 g         3773 t
ORIGIN      
        1 aaaaaaataa tcatacatag gagaaaaaaa acaaaataga aaaaagtgat tttctaataa
       61 attattcatg tggaaatgtc atttttactg tataattcat ttgattatcc attttttatt
      121 catcaaaatt actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa attcttcagg
      181 aaaaccgaaa cgcgacttca ctaaattatc gacaccccat acgtatgtca ttatttttgg
      241 tgtagtcatc tttgcatgga tactgacttt tgtcgttcca gcaggaaagt tcagcacgca
      301 agatattgaa tacaaagatg caaatggcga aacaagtact cgtactgttt tacgtcaaga
      361 ttccttccgt tatgcttacg agctggataa gtcgtatgtg tttgatcaat tagaagaact
      421 gcaagatcat ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat tggaaaaagt
      481 cattgctgac ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct ccttgacaga
      541 tgatgtgctt tacgatcagt acggcgaaaa tatctatgat acttctaaaa aattgcataa
      601 aacagccaaa atatggggaa cagatgattt tggcggcttt ggtttcttaa attttgtttt
      661 tgaaggacta gtatctggag ataagtatgg atctgctgta gggatcgctg cattgatcct
      721 tgttgttgga ggtgcttttg gtattattat gcgtaccgga gcgatcgacg caggtatcta
      781 tgcgtttatc agtaaaacta agggactgga gcgtttagct ttaccgctat tattctttgc
      841 tttttcattc ggtggcgcta cattcgggat ggcagaagag gtaattccat tctcaatggt
      901 gatggttcca tttgtcattg cgttaggata tgactcgatc gtagcagtaa ccgtgactta
      961 tgtcgcatct caagtcggaa atgcgacctc ttggatgagc ccgtttagcg tagcggtggc
     1021 tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga ttatgtgggt
     1081 agttgttact gcattagccg ctggttatat gatgatatat gcagaaaaaa tccgtaaaaa
     1141 accagaaagt tcgttgactt acaaatcaga tgattatttc cgttctcata tcaaaaagac
     1201 atctgatgaa aataaaaaat tcatgcttgg gcataaattg atcttattag agatgttagt
     1261 tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc tattacattc cagaaatcgc
     1321 ttctcaattc tttgtgatgg gattagttgc tggaatcatt gctgttcttt tcaaattaga
     1381 tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg atttagctgg
     1441 tacagcaatc gtagtaggta tggccaaagg gattcttctt gtcttgggag gatctgatgc
     1501 atctgttcct tcagctttga atacgatttt gcatgggatc gggacggcgt tgaccggtgt
     1561 tccagctgtc attggtgctt gggcgatgta catcttccaa agtttgttca acttggttgt
     1621 tacatcaaat tcaggacaag cagctttgac tatgccaatc atggctccac tggcagattt
     1681 agtaggtgta tccagacaag tagcagttct tgcttatcag cttggttcag gttttatgga
     1741 cgcttttaca cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac ggatcgaatg
     1801 ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc ctgtttgttt taggaactat
     1861 tttcatcatg attgccattg caataggtct gcagtagtac tggagacctg taaataaaaa
     1921 agtaatccaa acagtagata aggactccaa gaaccgtcat tttacggtaa aatcaaagga
     1981 gtaaagatcc tatcgtttgg attatttttt aagatcctat catcagttat acttaaagaa
     2041 agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag attacttagg
     2101 aaaaatggat gtactgattg ccggtgggaa gattttggct gtagaagaac agctagaagg
     2161 cggctacgaa ggagtggaag tagaagaact ttcaggtgag ggaaaagtac taaccccggg
     2221 tttcattgac tgtcatttcc atattttagg tggaggagga gaaggcggtt atcaaaatcg
     2281 aacgccagaa gtcacattga gccaattgac tacagctggt gtaacgacag ttgtcggttg
     2341 tttgggaaca gacggagaag gtagagatat gactgcgttg atcagcaaag cgaaaggtct
     2401 ggaagcagaa ggaatctcca cttatgtata cgaaggttcg taccgattgc cggtaaaaac
     2461 agtaacagat tctatcatca aagacttttt gacaattgat aaaattatcg gaatcggaga
     2521 aatcgcagta tccgatcacc gttcttctca gccaagtttt gaagagttcg ctcgtgcagt
     2581 agcagatgct cgtgtaggag gcatgttatc cggaaaagct gggatcatca atgtccactt
     2641 aggcggagga aaaagaaagc tagagctatt gacacgaatc gtagaagaaa cagaaattcc
     2701 gatcacacaa tttttgccta ctcatgcaaa tcgcacacca gaattatttg aagcttgcgt
     2761 ggcatttgcc aaacgtggtg gcacgattga cttcactgct agcgaagatc ctgatttttg
     2821 ggagaagaca gatggtgaag ttcgcttcag caaagcactg aaacgattga tcgaggaaga
     2881 tgtttcttta gataatttca cgatgacctc agatggacaa ggaagcttgc cttattttga
     2941 tgaaaacaat catttcctag ggttaggagt aggaagtgcc aaggcattgc tagtagggat
     3001 caaagaagct gtgcaaaaag aaagtatccc tttggaaatc gccttacgag ctataaccag
     3061 taatccagca cgtatattga aattggataa aaaaggaaaa atcgaaattg gtgctgatgc
     3121 agatctgtgt atcttggata aagaaacgct tgatattgat acagttatcg ctaaaggaga
     3181 aataatggtc caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa gtttttaatc
     3241 aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat ctgttgttga
     3301 tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat ggcagattta
     3361 aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga agcatacggc
     3421 aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact aggtgctgac
     3481 atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc tcgaaaagac
     3541 gaaaaaaaat aaaaaccact tattactaat tgaatcaagc aaataaaaaa tccgaaccat
     3601 atctgcgaga cagatatggt tcggattttt tatttgatca ttatcctgtt ttttaaactt
     3661 cgattttttg aaatttttta ttttttagtt caaagacttc atcacagctt tttgctactt
     3721 caatctcatg agtcacgatg ataatacagc gattttgttc atgggcgatt tcttggaaaa
     3781 gtttgacgat atcttgtgaa gttgtttcat ctagatttcc agtaggctca tccgctacga
     3841 ttagttgatg ttcacagcag atagcacgaa cgatagcgac tcgctgctgc tgacctcccg
     3901 aaagtttggt cacctgtttt tttgctaata tttcatcaat tcctacacga gccagtcgat
     3961 ctaaagcaac ttgtcgataa ttctcttgct tggtcttagt gatttccatt gctgctatga
     4021 cgttgtctag agccgataaa taaggaagca agttgtaagc ttggaaaata atcgagacat
     4081 catttttacg ataacttctt aacccgattt tgttcagtga acgatcttga tagtatacct
     4141 ttccttcttt tggtgcatct agtccagaaa taagcgaaag aaaagtcgtt tttccagaac
     4201 cacttgctcc taaaatagcg tacatttttc cagcttcgaa attcagttct acatctttat
     4261 aaagcgcatc ttgcggatct tgataccaaa atcccaattg ttctgtccgt aacatcaaat
     4321 tcctccttta gtttaataga atttttcttg ggtttaatct taatatccct atagaagcta
     4381 aaaagacaga aaacagactg attccaaatg ctactccggc taatagtccc aattgtgcag
     4441 gtttgacatt gatttccaat tcgctgactt gatcagaaac agcaaatgga ttattcgtgg
     4501 ttccttgttg accatcgcgt tgaccgcctt gttcttgtct gttacctggc atttgtccac
     4561 cgccaggagc ttgctggttt tgtgtcgtgg tttctgtctg ttggtcaagt agctgctgtc
     4621 ccacggcatt cgcgacgacg tttccgctaa gcgaagcaac tcctaatgca agaatcatac
     4681 aaatagccac ttcggtaaac aattggagaa tgattttact tctggattct cctaaagata
     4741 gcaagacgcc taactcatgc cgacgctccc gaatggtaat cataatgatc agagtaagta
     4801 tgataatacc ggctacagcc actagtaaaa caacattttt agaaaaactt gccacgttgt
     4861 ttaaaggttc caacattgac tgatacatgg aatcatttga ttgtaagcta aatgtatctg
     4921 tgtcgatcag cttctcagct tctttgagaa actcatccat tttatctgga tcagatagag
     4981 tgtaaatagc agaatctatc gtgttatcgt cagatgtccc atttagttca tttgcaaaag
     5041 tgtaagaagt gtaaagtgta ttcgacggat tcatgaagtt gaatttcata cccatactgc
     5101 cgcttgtttc tctggattca taaattcctt tgatcgtcat ttcataagtg gtatcttcat
     5161 cttcagtact cgtgattgtg aaagtatctc cgactgccag gtcatttgtt tctgctagag
     5221 tagaatcaat gactacattg tttgtgcctt tgtcgctgtc atctattcct tcaccatccg
     5281 tgatttttgc tgttccatca gaaaaattac tattttgtgc agttgctgac acaccactga
     5341 tttgaaagtc tgcttgagtc atttgcggat tcccgcccat tcctccagca ggttcaccgc
     5401 ctgcatcagt agtttctgaa ctgtcttcac ttgaatcaga actagtgatg gcagttattc
     5461 catcttttgc catagctgag gcggaacttt caaaggaata actctttaca ttatctaatt
     5521 cagctatttt ttccgcgtcc gttaaggaaa ctggtgtaag agaaaaactg cctggatctg
     5581 gacgttcttc agtggtagaa ccgcttgaag aagtttgttc ctgtttttta aatgaagctt
     5641 ctcgattggc acttaaagtc actgttgctc caacttcttt ttgtgcttga ttcgctgctt
     5701 cttctgcagc gcttcgaatc gtgaggccag ctagcacaaa aataagaatg gctgtgaaca
     5761 cggcaaacaa taacaatgaa cgtccccatt ttacttttgt acttttccat gcacgtttca
     5821 taaaattcat attttgcatc cctcctagta gatgcatttg ataatagaca acctatctta
     5881 aagctagctt aaatcttatg ctgtttgcac accatcaaca tgaggttctg tatatataaa
     5941 aagtcactat agttattgca aaaaatgtgg taaaatgaaa aaagtatgtt tcaaaccgac
     6001 gaagaagaaa tgaaagagga atagcaaaag aaatcctaga tgctacagag ggatttttac
     6061 gtgcatagtt gttctaacaa ttgctgatac tagcgattta gtttatttgt tgtataataa
     6121 tggggaacag ctgaatactt atataagtat tctggctata gcgtttcgca aaagatccat
     6181 tttgttaatg aagtatatcc aaaataaaat gcccatccaa ctgtcagaac gcggatgtgc
     6241 tggtaatttt cctgctgatt ctccataagt tgagaaatag tgattttgca tatgcttcat
     6301 tggaatggct catatattta gataaagaag aaaatcgaca tctttaaact tggatgtcat
     6361 aagaagaggt gaagtttgtg agtaccatta aaattatccc attaggcggc gttcgtgaaa
     6421 atggaaaaaa catgtatgtc gcagaagtgg gcgaggatat ttttatttta gattgtgggc
     6481 taaaatatcc tgaaaatgag ttgttgggga tcgacgtcgt aatccctgat tttacgtatt
     6541 tagaagaaaa cattgatcgt gtagctggtg tctttttaac acatggacat gcagatgcga
     6601 tcggggcact tccttacttg ttgtcaaaaa tttctgtacc agtattcggg acagagttga
     6661 cgatcgagtt agcaaaattg aatgtcagtc gtaatgaagc agccaaagga tttaaggatt
     6721 tccatgtggt agatgagcat acagaaatag actttggtga aacagtagtg agctttttcc
     6781 gtaccactca tacgatacca gattcagtgg ggatcaatct gaaaacaaaa gaaggtagta
     6841 tcgtttatac gggagatttt aaatttgacc aaacggctat cccaatgtat caaacagatt
     6901 atgcacgatt ggcggaaatc ggcaaagaag gagtcttagc cttactaagt gaatcggcaa
     6961 atgcagaaaa tcctcagcct gtagcttctg aagcccaaat agcagatgaa gtatttgata
     7021 cgatcaaata ttgggaaggg cgtatcatcg tcgcttgtgt agcaagtaat ttgcaacgag
     7081 tgcaacaagt attggatgca gcagaaaaag cacaacgaaa agttgtcttg actggtcaag
     7141 atttcgaacg aattattcgc acagcgatgc gtttagaaaa acttcaatta ccaagtgaag
     7201 atctgcttgt tacgttgaaa gacatgaaaa actatgatcc agaagaactg atcattttag
     7261 aaactggccg aatgggtgaa cccatcaagt ctctgcaaaa aatggcaaac ggtacacacc
     7321 gtaatgtcaa gattgaagaa ggagatttgg tttatatcac tactacgcca agcatcgcga
     7381 tggaaaccat cgtggcgaaa acggaagata tcatttatcg agctggtgga acagtgaagt
     7441 tgatctctga gaatatgcgc gtttctggcc atgccaatcc taatgacttg caattaatga
     7501 tcaatttgat gaaaccaaca tattttgtgc cagttcaagg agagtatcgt gaattagctg
     7561 ctcatgcaga tttggctcat gcagtaggga tgccatacaa aaatatctat attactggac
     7621 gcggagacgt gctggaatat aagaacaagc gtatgagtgt tgcaggaact acaagtgcag
     7681 aaaatgtaat gattgatgga ttaggcatag gtgatatcgg aaacattgtt ttacgtgatc
     7741 gaaaaatctt gtcagaagac gggattttcg tagcggttgt cactatcagt cggcgagaaa
     7801 agaaaatcat ttcaaaacct caaattactt cacggggatt cgtttacgta aaagctagcc
     7861 gtgatctgat gaaagaaagc agcaatatga ttgaaacaat cgttgagaaa catttagaaa
     7921 gcaatgattt tgagtggagc aaattaaagc aagacatccg agatcaatta ggccgttact
     7981 tatttgaaca aacaaaacgc cgcccagtga tcttgccagt cattatggaa gctactcaac
     8041 gtcgaaataa aaaataaaca aaaagagtaa gatgaacctc tctatagagt gctcgtcttg
     8101 ctcttttttt gcgaaaaaca aataattttt gaatacagca tccctcgctt tataataaac
     8161 gatagaataa agggagtgaa tcaatgatga gtcaaacaaa agtcgaacgt tatttagaag
     8221 aacaaaagat cagctatcaa ccacttgatt ttagtcatgt ggtagaagaa tctttcacag
     8281 aagaactaca aaaaagaggg attgatccag ctttgatatg taaaactctc gtagtgaaag
     8341 gaaacaaaac tggggtgatc atcgcattga ttcctttgaa cgatcactta gattacaaaa
     8401 aaacgagaca agtgacgaaa gatcgtaaag ttggttttcc tggaatggat tttgtattaa
     8461 tgcatacagg atatccccac ggagcaaaca cccctattgg aataaaactc gcacatcctg
     8521 attattgttt tttagcagac agttctttaa aagaaaaaga agaagtaatt gtgtcaagcg
     8581 gtgaaatcgg gagaagcctt cggataaaag tagccgattt agaaaagatc gtccatccta
     8641 tttacgctga tttaattagt taagggacga gaagagaagc taagcagagc agagacctca
     8701 aacttgagat tatatctgct tggtacttgg tgcatcctgt attatctgtt acaattgctt
     8761 tgtacatact gtactatcaa tccaatgaaa atatatttag cagctatatt ttttcattga
     8821 tgtgcaagtg atgaaatcgt ttttaataaa aaagatacag agaaaacgcg aggtgtaaaa
     8881 tatgaaaatt acgtatcatg gacattcctg tttaacaatt gaaatggaaa atggccaacg
     8941 cgtgatgatt gacccgttta ttacaggaaa tccattgaca gacttaactg cagaagaagt
     9001 aaagacagat tggttgctga ttactcatgg tcatagtgac cacataggag atatgattcc
     9061 tattgcaaaa gaaaatcaag ccacaattat cagtattgtt gagattgctg attatgcgaa
     9121 cagcagagga gtagattcat tcggtatgaa tatcggtgga aaacatgcgt ttccattcgg
     9181 cgtggtaaaa ttcgtacatg cacagcatag ttcaagctat gaagtagacg gtatcgttca
     9241 gtatatgggc gagcctagcg gaattattat tcaagcagaa ggaaaaacga tttatcatgc
     9301 aggggatact gcttatttca gtgatctagg attgcttgca gaagaattcg atattgatgt
     9361 cgctttcttg ccgattggcg ataactatac gatggggcca gaagatgcag caaaggctgc
     9421 gcaaacaatt cgtgccaaga aagtagttcc aatccattac aatactttcc ctgtcattga
     9481 acaagatccc gaatcttttg tagagttact ggaagatagt gaaggaaaaa taatgagcgt
     9541 ctcggaaacg ctggaagtgt aggcagaaaa tggcgacaaa acatgatttg attttagaat
     9601 atattgaaag tttacctgtt ggcaatcgaa tttctgtaag aagcatcgca aaagaattga
     9661 atgtcagtga aggaacagcg taccgtgcaa tcaaagatgc agaaaatgtc ggactagttt
     9721 caacaattca acgagtaggg accatccgta tcgaacgcaa attaaaaaaa catattgaac
     9781 gtttgacatt tggcgaggtc gtcaggatca ttgaagggga cgttttaggc ggggcggcag
     9841 gattagacaa ggtcttgaat aaattcgtca tcggcgcgat gactaaacat gccatggaac
     9901 gctatattac gccaggatca ctaatgatcg tcggcaaccg ggcggaagtc caaaaattag
     9961 cattggaaga tggcgccgca gtattgatca caggaggatt caatacgact gctgagattg
    10021 caaagctagc tgatgatctt gaaatgccga tcttacgcac ctcttatgat acatttacag
    10081 tcgcaaccat gatcaaccga gcgttaagcg accaattgat caaaaaagat atcatgcttg
    10141 tcagtgatat ctatatgcca gctgaaaaga ctcattattt gcagcagaca gatacgattt
    10201 ctgattatca gcgtttgtca gaagaaacgc agcattcgcg ttttccagtc gtcaaccgcc
    10261 atcatcgttt gatggggatt gttacggcaa aagatgtttt agggaaatcg ccaaaccaga
    10321 tcatcgatcg ggtgatgacg aaagaaccaa tcagtgtaaa gaaaacaatg agtattgcct
    10381 ctgttagcca tcaaatgatc tgggatgggc tagaagtcat gccggtcgtt tccgatgact
    10441 tgacattgga aggcttgatt acccggcaag atgtaatgaa agcgatgcag cttgtccagc
    10501 gacaaccgca gattgcagat acgatttcgg atcagatatc tggaaatatc catacgattg
    10561 atactgaccg tgaaggaaat cgtttggaaa accctaagtt caaattcgta gtagcaccac
    10621 aaatggtcaa tggcgttgga accatttctt ttggcgtact tagcgaaatc atttctgatg
    10681 tcgcacaaaa aacaatggtg atgaaccaaa aacgaaatat cttgattgaa caagtgaacc
    10741 tacattattt gcggctgatc caattggaaa gcgaattaga cattcgtcca cgtgtattgg
    10801 aaatcggcag acgttccgct aaactggata tcgaagtgtt tattgaaaac accatcgttt
    10861 ccaaagcaat cgttgtttgt caagtaatgg aacgttctta gaaagcagga gtgaaatgac
    10921 agtacagcaa gaaatagtag atacaataaa aaaatacgat aaaatcatta tccacaggca
    10981 tttgcgccct gatcctgatg cattaggatc acaagtagga ttagccgaac tattacggga
    11041 aagtttccca gaaaaagaaa tacgtcaagt aggtgaaacg attgaagggc ttcgcttttt
    11101 agcagaaatg caagaagttg aagatgtctt ctaccaagga gcgctagtca tcgtgactga
    11161 tacagctaat tcacccagaa tcagtgatca aagatatcag ttggcagaaa aactgatcaa
    11221 aatcgaccat catcccaatg atgaccctta tggagatctt gtgtgggtaa atacaaaagc
    11281 aagtagctgt agtgagatga ttgcagaatt tgctttgatg tttcctgaat ctttaaagat
    11341 gaacgttaag gctgcacggt tactttatgc aggaatcgtc ggagatacag ggcgcttttt
    11401 gtatccctcc acgacgtccc gtacattaga gattgcttcg atattgagaa attatccatt
    11461 tgatgcaagt gctttgaaca gagagattga acaaatgccg atgaaagtcg ctaaactttc
    11521 cggttatctt tatcaaaata tccaggtgga tgaaaatggt gcaggaagaa taatccttcc
    11581 gcaagaaatt cttgataaat atggaatcga tgattcagag acagcttcga tcgtttcact
    11641 tcctggagtg attgacgaag tgctggcttg ggggattttt gttcagcagc cttcagggta
    11701 ttaccgcgta cgtttacgtt ctaaggggcc gatcatcaat gaattggcta aacgccatca
    11761 tggaggaggc catccattag caagtggtgc gaatgcaaaa gatttagcag aagtagagga
    11821 aatctataaa gagatccaag atatatgcca atcgtataag agcagaaaaa cagaatgata
    11881 cttcgttcga aaacgtgcct tttgtagtaa aaatcacaaa tgtatctttt caagatcttc
    11941 tttttccgtt acaatagtgc tcactaaaga ttttaaagga gaaagaagat gacattacca
    12001 aattgcccag aatgtggctc agagtacaca tatgaagatc gaggcttgtt gatttgccca
    12061 gaatgcggga acgaatggaa caaagaagaa caagaaactg tcatagaaga cggattagtg
    12121 gtattagatg caaatggaaa ccgtctgtta gagggcgata gcgtgacagt gattaaagac
    12181 ttgaaagtaa aaggtgcgtc aggtgccatt aaacaaggaa caaaagtcaa gaacatccgt
    12241 ttagtagaag gcgatcataa tatcgattgc aaggtagaag gatttggacc gatgaaactg
    12301 aaatcggaat ttgtgaaaaa gaattaaaga atattcaagg tcttttcaca ttcttttcct
    12361 actactaatg ataaaataaa gtaaacgaaa aggagtgaaa cgaaaatgta tggttatgga
    12421 atgtatggtt ttttagatcc gacatttctt ttggtcattc ttggattagt tatttccgga
    12481 attgcgtcag cgtacgtcaa tagtactttc cgaaaatacg atcaagtgag aagcaaaaac
    12541 aatgtgactg gtacacaagc agcacaatat atattacaaa gtcaacagat aaataatgtc
    12601 ggtgttcaac aaattgccgg agatctcaca gataactaca attcaggtaa taagatgttg
    12661 agtttgtctc aagcaactgc acagtctact tcagttgctg cgataggtgt agcagctcat
    12721 gaatgcggac atgcggtaca agatgcttcg ggttatgtcc cattgaagat tcgtgcagca
    12781 cttgttccag cagctaactt tggttcgatg atttcgttcc ccttgattat ggtaggtgta
    12841 ttgcttagct ggaactctac gttaatcaat atcgggattt tcgctttttc tttagcatta
    12901 cttttccaat tggtgacatt accagtcgaa tttaacgcgt ctcggcgagc aatccaaatt
    12961 ttgagtgaag gcggactact gacggaagaa gaagtaccga tggcaagaca cgtattattt
    13021 gcagcagcac tgacttatgt tgcagcagcg ctttcgacat tcctgcagct gttgcgcttg
    13081 atcttattgt tcggcggcaa ccgaagagat taaaataaag aagttgtgaa atcagcgctt
    13141 tgatctgagc taataccgag agttagctga ttgaatacca tttatttgga a
//
LOCUS       DAZKLA010000072        13186 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000072 DAZKLA010000000
VERSION     DAZKLA010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.073"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(224..296)
                     /locus_tag="ACG85N_001810"
     tRNA            complement(224..296)
                     /locus_tag="ACG85N_001810"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(261..263),aa:Lys,seq:ctt)
     gene            677..1246
                     /locus_tag="ACG85N_001811"
     CDS             677..1246
                     /locus_tag="ACG85N_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFQ0224575.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1233..2093
                     /locus_tag="ACG85N_001812"
     CDS             1233..2093
                     /locus_tag="ACG85N_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0224576.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2283..2987
                     /gene="yycF"
                     /locus_tag="ACG85N_001813"
     CDS             2283..2987
                     /gene="yycF"
                     /locus_tag="ACG85N_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFQ0224577.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2992..4827
                     /gene="walK"
                     /locus_tag="ACG85N_001814"
     CDS             2992..4827
                     /gene="walK"
                     /locus_tag="ACG85N_001814"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFQ0224578.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4824..6140
                     /gene="yycH"
                     /locus_tag="ACG85N_001815"
     CDS             4824..6140
                     /gene="yycH"
                     /locus_tag="ACG85N_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFQ0224579.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6141..7010
                     /locus_tag="ACG85N_001816"
     CDS             6141..7010
                     /locus_tag="ACG85N_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFQ0224580.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7065..7874
                     /locus_tag="ACG85N_001817"
     CDS             7065..7874
                     /locus_tag="ACG85N_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0224581.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7977..8606)
                     /locus_tag="ACG85N_001818"
     CDS             complement(7977..8606)
                     /locus_tag="ACG85N_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0224582.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8776..10068
                     /locus_tag="ACG85N_001819"
     CDS             8776..10068
                     /locus_tag="ACG85N_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0224583.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10144..10806)
                     /locus_tag="ACG85N_001820"
     CDS             complement(10144..10806)
                     /locus_tag="ACG85N_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0224584.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11031..11315
                     /gene="groES"
                     /locus_tag="ACG85N_001821"
     CDS             11031..11315
                     /gene="groES"
                     /locus_tag="ACG85N_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFQ0224585.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11366..12991
                     /gene="groL"
                     /locus_tag="ACG85N_001822"
     CDS             11366..12991
                     /gene="groL"
                     /locus_tag="ACG85N_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFQ0224586.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4386 a         2277 c         2842 g         3681 t
ORIGIN      
        1 ttggcagcat gattttgcaa agcttctcga attattgtta tattagaaac tttactgttc
       61 ataattatgc ttctttttta actcaaagtc caaagggcta cagtggactt tgagttaaaa
      121 ataaaaagcc ctagttattt tgaaaaataa aaaaagaaca gcgtaaatgt tttcgaaatg
      181 ctttcgcacc aacatttacc tgttccgtgg gtttggcacc caatgggccg tgaggggctc
      241 gaacccgcga cccgctgatt aagagtcagc tgctctacca actgagctaa cggcccgtac
      301 aaaaagtact taataatagt agcactaaga attttaaaat gcaagactac atttaaagaa
      361 aatcagaaaa atctttactt ccttatagga aatcattcat agattattca catttgctcg
      421 ctattatata agcataccaa caaacaaagt tcttgcaaga gaacaaaccc taacactttt
      481 ttcatttttt actcctttcc cgccgtctcc ccggcgggtc tcctgttctt aacgataaaa
      541 gacaagaagg cttagccttc ttgttttttg ttttcatgga atcccgctaa tcaaatcacc
      601 tagttaaagc aactagattg aatgtttatc ataagtctga tatcatgttt atgaaatgaa
      661 aaaaggagcg gttatgatgg aaaaaatgcg tcagcaatta atggagtggg ggaaggaact
      721 agaaacattc cgtctgcctc actgggaaga actccctgac ttagaacttt atatggacca
      781 agtcagaacg ttagttgatc gttatctttc acctgtgata caaggagaaa agcatccatt
      841 gcttacctct tctatggtaa acaattacgt taaactaggt ttgataccag caccagttaa
      901 aaaaagatat aacaaagaac acgtagcatt tttacttgca atcactacgt tgaaacaagt
      961 attgactatt cctgaaatca aagaagggat attgttccaa ggaaaaacag taggaattcg
     1021 tgaagcctat aatctatttt gcgatgaaca agaagcagct gtgtggatgg tcagccaatt
     1081 agcacaaggt aaatctcatc cgcaaaaatt cgaccaggca acccctgtag aatatattgc
     1141 tgtaagagcg gcaactttat cgtttgcaat gaaacttctt gcggaaaaaa cgattgttct
     1201 agaaaccgaa tatttaaaag aggagaaaac aaatgaaaaa caataaaatc gcaattttag
     1261 tagattcagg tacggatgtt ccagaagaat tgataaaaaa atacgagatg tatatgatcc
     1321 cattgaaaat tatttataaa gatcaggtat atacagataa aattgacatt acccctgaag
     1381 agatctatca acgtttgcca gaggaaatcc ctggtacatc tttgccggat ggtgagtcga
     1441 tcacagaaat ctttgagcaa atcaaaaaag acggttatga aaaggtactt gcggtaacaa
     1501 tttccagtgg attgagcggg acatataatg tggttcgttt aattgcacaa cagcaagaag
     1561 aattggaagc atatgtacta gatacgaaaa atattggtat tggtgcaggt ttttctgcga
     1621 tccaagcagc aaaatggctg gaagaaggag tagaatggga tcaactgatc agtaatctga
     1681 atgagttagt cgaaagaaca aaagttttct ttaatgttgc gacattggaa tatcttcaaa
     1741 aaggtggacg gatcggttta gttgcttcta ttgtaggaac cgctttaaag cttaacccaa
     1801 tcatctcatg taatgaagaa gggatttatt acacagtagg aaaagctagg ggacgtaaaa
     1861 aaagtttgga taaaacggtt gcttacgtca aagaaaaaat cggtaatgca aaggtcttca
     1921 acttagctgt tgcccatggg gatgcgaaag aagaagcaat tgaaatggaa gcgagactta
     1981 aaagagagtt cccacaagca aaccaaatct actttggcca gatttctccg gcattagtag
     2041 tccacactgg tccgggatta ttgggtgtcg gtgtacaagt tttagaagga taaatcaaaa
     2101 aaaggaaaca ctgaggtgaa aatcagtgtt ttcctttttt attgaagtta gaacaaaaaa
     2161 taataaagta ttttgcactt tcattttaat tttctctttt gttttatagg caaagatgaa
     2221 acaaaagcag aaatacggta taatgagaag gtatgacaat aatatataag aggtggctct
     2281 ttatgaaaaa agttttagtt gtcgatgatg agaagccgat ttcggatatc gtcaaattca
     2341 atttagccaa agaaggctat gacgtttata ctgcatatga tggggaagaa gccttggaaa
     2401 aagttgctga ggtagaacct gatttgattt tacttgattt gatgctacct aaaatggacg
     2461 gcttagaagt tgctcgtgag gtacgcaaaa cttatgatat gccgatcatt atggtaacag
     2521 ccaaagattc tgaaattgat aaagtcctag gcttagagtt aggcgccgat gattacgtga
     2581 ccaagccatt ttccaaccga gaattagttg cacgtgtcaa agcaaatctt cgtcgcggtg
     2641 ccacggctgc gaaggaacca gaagaggcag cacctgcgga attgacgatt ggtgatttga
     2701 cgatccatcc agaagcttac atggtgacga aacgaggaga aacaatcgag ttaacacatc
     2761 gtgaattcga actactcttt tacttggcga agcaccttgg acaagtgatg acaagagaac
     2821 atttattaca aacggtttgg ggttatgatt attttggaga tgtacgaaca gtggatgtaa
     2881 ctgtacgtcg tttgcgtgaa aagatcgaag ataatccaag ccatccaaat tatttagtga
     2941 cacgtcgtgg tgtcggttac tatttgcgta acccagaaca ggagtaattg gatgaaaaga
     3001 aaagtccgct tttttcgatc agtcaatttt aagatcgcta ttacctttat tctgatttta
     3061 ttgatctcga tcgaaattat tggtgcctac tttatacgtg gtttagaacg ttccacgatc
     3121 aatacattta ttaaagatat gaatcaaacg gtggaatcat tagcaacaac aatcagcccg
     3181 gaattgaatc gaaaagataa tgcagatgat gaagaggtaa atgcgaatat caaaagattt
     3241 attgaaaaca gtgcgactag cgatatcatc gaaatacggg tcgttgacga aaaagggatc
     3301 atccgaggaa caaccgatgt caatgaacaa agcgctgtgg gaaagaaaaa tgattatgtt
     3361 gatatcaatg atttcacgac taaacgttat gttgctttag ataacgacga taaacgggtg
     3421 aatatcaatg ttcaaccgat cctgtccccg accggcgatg ccgttatcgg ggctctttac
     3481 gtcaaaagta atatcgaaca aaaatattcg gaaatcaata acacagcagt cattttcttt
     3541 actgcctcac taattgcggc atttatttca atgatcgtat ctgttttagt tgctcgttcg
     3601 attacacagc cgatcggtga aatgagagaa caagccattc gaatcgctag aggagactac
     3661 agtcggaaag tcaaagtgca tggacaagat gaacttggac agctggctga taccttcaat
     3721 cagttagctg aacgtattga agaagctcaa gatacaatgg aagctgaacg caatcgtttg
     3781 gatagcgtgc tatcccatat gacagacggc gtcattgcga cagatcgcag gggtaaagtc
     3841 attacgatca atgatatggc tgtttccttg ctagatatca aaaacgagga agcaatcgga
     3901 caatcgattt tgacactttt agatatcgaa gaagaatata cgttgcggaa attattagaa
     3961 gatccaaacg aaatggtact tgaccgttca accagtacat tggaaagtga tcagatgatc
     4021 ttgcgagtag acttttctat gatccgtcgc gaatcaggtt ttatcagcgg attagtagct
     4081 gtcttgcatg atgtgacaga acaagaaaaa acagaacggg aacgtcgtga attcgtctct
     4141 aatgtttccc atgaacttcg tacaccgttg acaagtatgc gtagttatat cgaagcattg
     4201 agcgaaggtg cgtggaaaga cgaggaagta gcaccaaatt ttttgaaggt aacgctagat
     4261 gaaacagacc gaatgattcg aatgatcaat gacctcttaa atctatctcg catggataca
     4321 ggtaataccc agcttcaatt agaatatgtc aatttcaacg agttagtgaa ttttgttctt
     4381 gaccggtttg atatgatggt gggaaatcag gaaaaaaatt ataagatccg tcgtgaattt
     4441 acgcaaagag atctttgggt cgaagtagat acagataaaa tcatccaagt agtcgataat
     4501 atcatgaata acgcgatcaa gtattcacca gatgggggaa cgattacttg tcgcttatta
     4561 gaaacgcata ataacgtgat tttaagtatc accgatcaag gattggggat tcctaaaaaa
     4621 gatctgaacc gagtgttcga gcggttctat cgagtagata aagcacgtgc aagagcacaa
     4681 ggtgggacag gacttggatt agctatttct agagaagtga ttaaggcgca tcgaggcgct
     4741 atttgggcag agagcaagga aggaaaaggg tcgacttttt atatctcgct gccatatgaa
     4801 ccttatgagg aggaatggtg ggaatgagaa tctctgaaaa agtcgttcgt ataggactca
     4861 ttttgatggt tgcgttaagc atatacttct cttatgcaat ctggctgagc ccagcaggaa
     4921 aaagttcaat caacgtagat gagacaaaca ctcaaatgat cgagtctcaa agcaatcgaa
     4981 aaacttccga agtctttctt ccgcttcatg ttacatggca ccattcggga atgatccaag
     5041 aaaccaacag tgaaaatctc gtgtaccgat tgcaattgat catcgataaa gcaagatttg
     5101 gaaagctttc tgaagtagtt tcaggagaca aatcacagtt tgaacaggtg aaaaatctga
     5161 actctggttt tgagttggcc tataatgcgc cttttttgct aagagaatac aaggaagcat
     5221 ttgatttaga tttagatgtg tcgaacttgg caaaagaaag cgaaaatatg tattttacta
     5281 gaatacaatt cgatcaagga aataaaaaaa tccgattttt gaattattcg aaccatctcg
     5341 tatatgaagc aaatatttcg ttgaattgga cagatatcaa taaagaatta acagcaaaag
     5401 acacaaaatg gacagaaatg acaacagaac catcgacgat cgacacgcaa tatgctacga
     5461 aacattcgat caaactgaag aagtatagtt atatcctgtc acagcaaccc tatacacttt
     5521 ttagaaatgc ttttttccag aaaccagata gcgtgaaaaa taatgatgaa agcaatgaat
     5581 tgatttttta cggtgggaat gaaacaatgt ctatccattc ggaaacgcaa gtagttgatt
     5641 ttcgtggaga gctgccagag aaaaaagaaa atgctagtgt attttctgaa agtttcccct
     5701 atattagcaa attaggcagt tctttaggga acctcagata ttttgaccgc agtgacaata
     5761 cgatcgatta tcggatattc gtggaaggct tcccggtttt tgggacagac agcagaggac
     5821 agattggtgt ggaagttggc ggtgaaacga atggttctgt gcaagtgaat atccaaacca
     5881 gtttgaatac gattcaagtg ccgatacctt cagacgaaga agtagaactt gcaagtacga
     5941 atgaaatcat ccagcagtta aaagctaagg gagcagatag caacaagatt ggttcagtga
     6001 tcgttggcta tacatggcaa aatgtcaaag aaacgaatcg tgtcgttgat ctcttacctg
     6061 aatggtatgt gaaatataaa gataactggt attcagctaa cgaccttctg gaaaaattac
     6121 ctggattgga ggcgaattaa ttggatttca aaaaaatcga atggatattt ttcgtcgctt
     6181 ttttagggtt gaatgtgttt ttattcagca gttatcatga cgcggtatac caagaaaaca
     6241 atgtcattcg ctctaatcag actgaatcaa tccaacaacg tctggctagc gacgatatca
     6301 cttacacggg gaagatttcg tctgagaaca agcaaggcta ttatcttagc gcggagcaga
     6361 ccaatcttag cacttcatta gcgaattatc gcaaggctgg taaaagcggc ttgctggcaa
     6421 gtggtgtgtt tttagacggt gatgttttga cgaaatcttt atccagttca gatgctgaaa
     6481 agaacgtgat cgatccagat aaaattagta gttccttgga agatttttta tctaacaaag
     6541 acaatatttt attcggtaat gattatgtgt acacgaaaaa cttttctcat ttagaagaag
     6601 aaccactaga aatccaagca agccaagagt atgaaggcat cccattcaat gatgatacag
     6661 caaaaatcgt tctttcttta gaaaaaaacg ataatgatta tagcatcacg aaatacatgc
     6721 agacgcattt atctgatatc gagcagctgc gcgaaaagac ggaactgtat acggaagagg
     6781 aagcaatcaa tacattgtac atcaataaca aaataccaag aggatcaaaa attttatggc
     6841 gtcagctagc ttattcctgt attctgaaag tccgggaaaa aaatgtgtat gttcctgttt
     6901 ggcatgtagc gatcgaaaca agcgataaag tggtccaaat tgagagcgtc aacgctttca
     6961 gcaatacgat cgtgacaaac aatactgtac caaaagtgga agatcaataa aaaagcgagt
     7021 ataatgaaaa aagtttacaa agtaagaagg gaagtaggag tcaaatggct gaagaaaaac
     7081 cagcttttaa aatcagtatc ctagcaagtg gaagttcggg taattcactt tatatcgaaa
     7141 gtgaaaaaaa acggcttttg gtcgatgcag gacttagcgg taaaaaaatc acttccttga
     7201 tggctcaaat tgatcggaca ccggaccaat tagatggaat acttgtgacg catgaacatc
     7261 gagatcatat acatggcgtg ggtgtcttgg ctcgtaagta tcatttggat gtttttgcca
     7321 atcagcagac ttgggaagcg atggaaccac tgattggaaa tgtaccagtg gaacaaaaac
     7381 atctatttga gatggggaaa gtcagaacat tcggcgatat cgatattgag agtttcggcg
     7441 tctcccatga cgcagcggct ccgcaatttt atcgtttcca taaagaaaat cggtcattcg
     7501 tcatgttgac ggacaccgga tattgcagtg attatatgcg aggtgtcatt gaaaatgccg
     7561 atggttattt gatggaaagt aatcatgatc ttgagatgtt acgcatggga ccttatccgt
     7621 ggaatttaaa acagcgtatt ttaggagata gaggacatct ttccaacgaa gacggtgcac
     7681 ttgtcatgac tgatgtgatc ggtaatcgga ccaaacgaat ctatcttggc catcttagca
     7741 aggaaaacaa tatgaaagaa cttgctcatt taacaatgga aaatgttttg aaagaaaaag
     7801 attttggcgt tggacatgat tttgaaatct atgatacaga tccagatgaa gcaactgagc
     7861 tattttcgat ttaaacagtt caatcaataa aaatataaaa agcagaagat aaaatcaatc
     7921 agggttggtt ttatcttcta tttttatttt tttagcatat taaaaaaata accgtattat
     7981 cgactgacta cactgatgtg ctgcttttca tgtttctcat ccagtatctc atctactaat
     8041 gcaatcgcgt aatctgccat gctgatcgta ctgtcgctag cagcgtttct ttgtaaataa
     8101 tcatcactta acaaataagt acctgtacgt ttgccatgtg gatcaaataa agaagctgga
     8161 ctgatataag tccaatgtag atttttagat tgtttcaatt ttaacaatcc ttcgtacata
     8221 ttgaaagctg ttggatagaa gggtgcattt ttaggtactt ggtcaatcat acgagtcgtc
     8281 ttttctgtat ctaagaataa cgaagaggca cccccaacga caattaagcg tgtatcggtt
     8341 ccttcaagca tctcaatcaa atgggacaaa gaagtttgat gcatttcttc ttttcctttg
     8401 ggagcgttga aagcatcaac gacaacatca aaagatgcaa gatcagtagt tgtcaactca
     8461 aataaatcct tttgtataaa cggaacatct gatggcaatt tttctggatg acgcacgatt
     8521 gccgtcacgt ctaaatctct ggacaatgct tcgtctaaaa taaatgaacc tgcatggcca
     8581 gtagctccaa tgagtgcaat tttcataaaa aacctccttt tttattcctg tccattgtaa
     8641 gcagttgctg aatattcgtc aaagaatccg atttttgaaa agctcagtgc gcattccttt
     8701 catggttcgt gatcattggg tatagttaaa ttaagaaaac tgattgataa aaatagaagt
     8761 tgaaggagat aagaaatgaa agagtggtta gatcgtgcct ttttaccaat gataacatct
     8821 gttgtgaatc ttagaccctt aactgctttg cgtaatggaa tgacttacac gatcccaata
     8881 attattgttg gctcgctgtt tttcttattt gctcattttc ctgtggatta catcgctcag
     8941 tggatagaca aaacaggatt ttctatgtat tttacacaag tataccaggc tacatttgat
     9001 gtgatagcct tttggatcgt ttttgccatt gcatacgctt atgcaaaaga agaagacatc
     9061 gatcgcttac cagtcggcat aacagccttg tctgcttatc tactgcttat tcagccacta
     9121 aatactcaga tagatactgt ctggacaggt gctgaaggga tactaggagc agttacaact
     9181 gggctcatca tcggatgggg ctatgcacgt ttagtaagac ttgcgaaagg gtggaagcaa
     9241 agagagaaga tacctgaaaa tgtttggcag tcttttactt cattgattcc aatcattgtt
     9301 atagtcactt ttacgcttat cggttcagcg gtttttcaac atttgttcag aatgactgtt
     9361 gttcaaggag tatggacata tgtccagctt cccattcaat cgttcataga cacgttgttt
     9421 ggtgtgcttt tattaggttt ttttgttccg tttttatggt tatttggtct tcatggatct
     9481 gcgatggtca atgggttggt tagtcccgtc ttgcaagcga attcattagc aaatgcagaa
     9541 atcttagcct caggacaaga gttgacggtg gcaaatggcg gacatatcgt tactcagcaa
     9601 tttctcgatc agttcatgac ggttacgggt gctggattga cgttaggggc tgtatttttt
     9661 atgacggttt ttgcgaaatc gaagaaatac agagaattag gaaatcttgc tcttttacca
     9721 gcctttttca acatcaatga atcaattatt ttttccacac cgatcgtgat gaacccgatg
     9781 atggctgttc cttttatttt cgcaccggtc gtttcaggat tgattaccta ttccgcttta
     9841 tattttggat tcgttccttt atttacagca gtccaagtcc cttggacaac accgccgatc
     9901 ctctcgggtt tcttagtagg cggagtgcct gcagcgattt tacaaggtat cgtgttgtca
     9961 attagttttt tcatttattt cccatttttg aaaaaaatag attctgcaaa ctgcaaaaag
    10021 gaaagacaga caaaaaatga tggagcagca gaaaagatga acgactaaaa aacaaaaata
    10081 ctcctgtcgc tttagattgg gaaaatcacc aatctaggat gacaggagta tttatagtca
    10141 tgtttatact agcccttttt ggacagctaa ttgaacaaga acaactaatg tgttcattcc
    10201 tacgtgtgtg atcatcgatg tccagatccg accagttgct ttgtactcta ggctgaagaa
    10261 gaaacctagg aaaaagtaga ttagcagatg accatcatta tgagcaaacg caaacagcaa
    10321 agagctgatc actgctccga cccaaaaatt tgaatatcga ctgatgatcc ccagtatact
    10381 gcggcggaat acgaactctt ccatgatggg tccagcaaca gttgtcgcca aaataaaagc
    10441 gggtgcttcc atgatcatct ggatgatatt ttgtgtattc tcggaagggg ttgctttgcc
    10501 aaacaataca gattctaaca tgacagcgat gctttgcaga atcaatgcga caaagattcc
    10561 aaccaatcca taaataacaa tctttggagc agaagctttt tttaccgctg attcgatcgc
    10621 taagggttca cgttgcttaa aataaaaaat agctaggaca acggcgccta gtatatacat
    10681 gactgcagtt gctgtgatca attcagacgt agattttaca agtcctatcg aagctaggat
    10741 caagggtgag ataaaaatta gaccatagca aaaaatgctg aggaagctat atttttttaa
    10801 agacatgtaa ttcctccttt taagggataa caagcatgca aggcaagctt atatcgattg
    10861 atttcaatta tatcataatt tcaataagca gctgtcagaa taaaatttct tttctttgat
    10921 ttaaaagctt gcaaaaaaga gggagaatga gtattattaa aaatgtaggt tagcactcga
    10981 aggttaagag tgctaacaga ttaaaaaaag agttatcgga gggatttatc gtgttaaaac
    11041 cattaggtga tcgcgtcatt atcgaagtcg caaaagaaga agaaaaaact gtcggaggaa
    11101 tcgttttagc ttctgctgct aaagagaaac cccaaactgg gacagttgtt gcagtcggtg
    11161 aaggccgttt gttagaaaac ggtgaaaaag ttcctgccgc tgtaaaagca ggcgaccaag
    11221 ttatgttcga aaaatacgcc ggtacagaag taaaatacga aggaaaagaa tatctgatcg
    11281 tcgcaggaaa agatatcatg gcgattgttg agtaaatttg gaagataaaa aaataatcaa
    11341 aaataatcaa tgaggtgaat caatcatggc aaaagaattg aaatttgcag aagacgcacg
    11401 cgcagctatg ttacgcgggg tagataaatt agcagataca gtgaaagtaa cattaggacc
    11461 taaaggccga aatgtcgtac tagaaaaatc ttacggctct ccattgatca caaatgatgg
    11521 tgtgacgatc gcaaaagaaa tcgaattaga agatcatttt gaaaacatgg gtgcaaaact
    11581 tgtatcagaa gtagcatcaa aaacaaatga tattgcagga gacggtacaa cgacagcaac
    11641 tgttctgaca caagcaatcg ttcgcgaagg tttaaaaaac gtaactgcag gtgctaatcc
    11701 tctaggcatt cgtcgcggga tcgaattggc aacaaaagca gcagtcgaag aattgcataa
    11761 tatctcaact gttgttgatt caaaagaagc cattgcacaa gtagcagctg tttcttcggg
    11821 ttctgataaa gttggacatt tgattgctga tgcaatggaa aaagttggaa acgacggtgt
    11881 catcacaatc gaagaatcaa aagggatcga aacagaacta gatgttgtag aaggtatgca
    11941 gtttgatcgt ggttacttat cacaatatat ggtaacagat aacgacaaaa tggaagctgt
    12001 tctagaaaac ccatatatct tgatcacaga caaaaaaatc tctaacatcc aagatattct
    12061 gccattgtta gaacaaatct tgcaacaatc acgtccattg ttgattattg ctgacgatgt
    12121 agatggtgaa gcattgccaa ctcttgtatt gaacaaaatc cgtggaacat tcaacgtagt
    12181 agctgtaaaa gctcctggtt tcggcgatcg tcgtaaagca atgcttgaag atatcgctat
    12241 tttgacaggt ggtacagtaa ttactgacga tctaggttta gaattgaaag atgcaacaat
    12301 cgaaaactta ggaaatgctt ctaaagttgt tgtagataaa gacaatacaa cgatcgtaga
    12361 aggttctgga gaaaaagaag caatcgaagc tcgtgtgcaa ttgatcaaaa accaaattgc
    12421 tgaaacaact tctgattttg accgcgaaaa attacaagaa cgcttagcta aattagcagg
    12481 tggtgtagct gttgttaaag ttggtgcagc tactgaaaca gaattgaaag aattaaaatt
    12541 acgcattgaa gatgcattga atgctacacg tgccgcagta gaagaaggaa tggtctcagg
    12601 tggtggtaca gctctagtga acgttatcag caaagtttct gctgtagaag cagaaggaga
    12661 cgtggcaaca ggtatcaaga tcgttgttcg tgctttagaa gaaccaattc gtcaaatcgc
    12721 tgaaaacgct ggttatgaag gatcagtgat tgttgataaa ttgaagaacg tggaactagg
    12781 cactggtttc aatgctgcta ctggagaatg ggtaaacatg gttgaagcag gtatcgtaga
    12841 cccaactaaa gtaacacgtt ctgccttgca aaatgccgct tccgtttctg ccttgttatt
    12901 aacgacagaa gcagtcgttg cagataaacc agaaccagca gcgccagcag ctcctgcaat
    12961 ggatccatca atgatgggcg gtatgatgta agaaaaagca acttgcttat ctacaactga
    13021 tcattagtga aagacagata aaaagaggct ggaacagaag cgtttaactc caagaaataa
    13081 gaagaaattc acggaaattg cttttcaaat ttttgtgaat ttcagcttat ttccgaagga
    13141 gttgcttctg cttccgctgt ttatacgtgt ttagagcgtg agacaa
//
LOCUS       DAZKLA010000073        12870 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000073 DAZKLA010000000
VERSION     DAZKLA010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.074"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..2275
                     /gene="acm"
                     /locus_tag="ACG85N_001823"
     CDS             110..2275
                     /gene="acm"
                     /locus_tag="ACG85N_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFQ0224587.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2547..3032)
                     /locus_tag="ACG85N_001824"
     CDS             complement(2547..3032)
                     /locus_tag="ACG85N_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFQ0224588.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3122..3467
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3512..4153
                     /locus_tag="ACG85N_001825"
     CDS             3512..4153
                     /locus_tag="ACG85N_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0224589.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4172..4807
                     /locus_tag="ACG85N_001826"
     CDS             4172..4807
                     /locus_tag="ACG85N_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224590.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4825..5640
                     /locus_tag="ACG85N_001827"
     CDS             4825..5640
                     /locus_tag="ACG85N_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0224591.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5959..7395
                     /locus_tag="ACG85N_001828"
     CDS             5959..7395
                     /locus_tag="ACG85N_001828"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0224592.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7584..10031
                     /locus_tag="ACG85N_001829"
     CDS             7584..10031
                     /locus_tag="ACG85N_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFQ0224593.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10261..11328
                     /gene="rlmN"
                     /locus_tag="ACG85N_001830"
     CDS             10261..11328
                     /gene="rlmN"
                     /locus_tag="ACG85N_001830"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFQ0224594.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11318..11524
                     /locus_tag="ACG85N_001831"
     CDS             11318..11524
                     /locus_tag="ACG85N_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224595.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11521..12624)
                     /locus_tag="ACG85N_001832"
     CDS             complement(11521..12624)
                     /locus_tag="ACG85N_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0224596.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         4394 a         2155 c         2740 g         3581 t
ORIGIN      
        1 attttttagt taaaagattg gaaatgagtg ggggaggaat tgaagaaagg atttttgaga
       61 gatgatatag taggttttat ttttagttaa aataggggag atagaaaaaa tgaaaaagtg
      121 tagaaatatt attttatcaa tgttatttat tattactaat ataacatcac tgattcctgt
      181 tcatgtctat gcggatgcag gcagagatat cagcagtaat gtcacttcgt tgaccgtgga
      241 cccaacgaat attacagatg gaggaaacat aaaagtaaaa ttctcgtttg acgaaaaaaa
      301 acaaaatatc caaccaggtg attatctttg gatcaattgg ccaagtgaag gaaatattcg
      361 cggtgaagga ttccaaaaag aaatcccatt aatgattgaa aataaaaatg ttggaacttt
      421 aacggtcaga aaagattctg cgcaggttgt gtttaatgaa aatattaaaa acctagactc
      481 agtagaaggt tgggggcaat ttgagataca ggccagaaac gtaaccgata ccggtgaaga
      541 aaatacggga ccattcaccg tgacgagcgg tgataaaaca gctactgtaa atgtgacaaa
      601 gccagcttct ggttcttctt ctagcgtttt ttattataaa acaggagaca tgctacctga
      661 agatactaag cacattcgat ggtttttgaa tattaacaat aacggcactt atgttgaaca
      721 accagttaaa atcagtgatg aaatccaaag tggacaaaga ttagatccaa gtacgtttga
      781 aattaatcaa atacacttag gtgaacagaa ggtttataga ggagaagagg gaatccaaca
      841 atttttacaa gatttcccca gcgctacatt taatttttca gtaactgata attacattga
      901 aataactata ccaaaaaact ttgtcaatct taggaaaata atggtgagtt ataagacgat
      961 catagaaaac ccagaacaaa taaattttga gaatcattcg gaagcttggt ttaaagaatt
     1021 taataaacct gctgttgatg gagaaagttt caatcataca gttaaaaata tttcagcttc
     1081 tggtggagtc aacggtacag ttagaggcga attgaagatt ttcaaatata tcaatgacac
     1141 agagattggt atcccaaatg tgactttcga attaagacga gcagatgaac agcctattca
     1201 aggacagtct agtatcctgt tgacttcgaa tgaacaggga gaagctagta tcaaaggcct
     1261 gcaagtagga gactatgttg taaaagaaaa agaagcaccc aattggattg attttgatcc
     1321 actaagttca aatgaactca aattctccat aaacgaaaat gatacagagg gagtcagttt
     1381 acctatttat aacaagaaaa aggttacgaa catcacagca actaaaattt ggaacggtgg
     1441 aaccactcca agaccgagta tttattttaa attattcaga gcaagtacaa acaattggga
     1501 accagttcct gatgcagaaa caaaacgatt agacaatgga ataacttcag tgacttggga
     1561 agatatccaa cagtatgatg atagtggaaa cgaatatacg tttaaagtgc aagaagtaga
     1621 tcaaaacgga aatgattatg tcccttcagg ttatcaaaaa attgaaaacg ggctagtcgt
     1681 tacaaatgag aataaagaag tcatttcagt ctcaggacaa aaaacatggg aagacaatga
     1741 gaaccaagac ggcaaacgac cgacgacgat cacagtaaat ctattagccg acggtcaacc
     1801 gatccaacac aaagaagtaa gtgaaaaaga tgattggcgc tatcgtttca caaatctgcc
     1861 aaaatacaga gacgggcaag aaatcatcta tactgtaacg gaagacaacg tgcccgaata
     1921 cagtacgacg attgacgggt ataacatcaa aaacagttat acaccggaaa aaacaaatat
     1981 atctatacca ggtagccata taacaccgtg gaaaaatttt ccagagaaga cagagagaga
     2041 gaaaattaga aatctattct ctcaaaaatt gcaaatattt gctcatacag gagaatccag
     2101 cgaacaaaga agcaattata ttagtaagag agctgtacct aaacagataa caaataaatc
     2161 aaaaagcatt cttcctaaaa caagtagtaa aaaatctagt tttgctgtaa tcattggttt
     2221 attgatagta actatctgtg ctgggatatt cttacaaaaa tataaaaaag tttagttaga
     2281 atataatatt ttttgttaaa tatgactgaa atcaaaagaa taaaattccg atttataatc
     2341 gaaaaaagaa gcatttttat atatgcttct tttttcgtag aatcaataaa tatggatttg
     2401 gctaactatg gaagaatact cgtattagtt ttcagcgttc atactagttg ttacattgtt
     2461 tgattggcca aaagaatttc tgatatattt tgtgctaaat aaagaaaaag acgcttgaat
     2521 tgggaaataa gaaaactact ctatcattac aaattttcat aaactggcag atgttctaag
     2581 taaacaagta tcttttcaaa gtcggaaaaa tccataatca acgtagtggt attgatattt
     2641 ggatgaaagc ctacggtatc ttgacgatca atagcagcat cgatcacgac ttggattttt
     2701 ttctctttgt cgtgcatcaa tgctaatgga gtcactgtac ctgctggaac gcctaggagg
     2761 tcaggaagct gttcttcaga aacaaaagat aagcgctttt cttttaattg ttcagctagt
     2821 tgttttaaat cggcgtgctt ttcatcaggc aagatgacaa gatagatttg ttttcctttt
     2881 cttgatttca ataatagatt ttttacttgc ggaccaggta gggaaaaagg gacatttttg
     2941 actgacgtaa tcgcaggatg atcgactttt tgaaaagaga tacccagttt ttttagtagt
     3001 tcataagctt cttgttcggt tgcacgactc atcgcgcatc accctccttt ttttattagt
     3061 gtatcataaa aaagagttga caaatgaaaa ataatcatgt aaattacacg taaagttaaa
     3121 acaaatgaca tcgaaaagaa gagtagccgt taggtgtttt tagagagtct ctggttggtg
     3181 gaaagaggca gcagttaacc gtgaaacaca tcttggagtc gattttctga agcgtatagt
     3241 aggaaaatcc ggggagcccg ttacagctgc ggtcgttgct agatgaccag ataaggctgg
     3301 cttttccgtg aggagtcggt aaaaaaaggt ggaaccacgt gattaacgtc ctttgatctt
     3361 cggatcaaag gactttttgt tttcatctga acggcaacga ctgtgtgagg ttttttctgc
     3421 aaggaaaaaa taaacagagg tggaaccacg tataatacgt cctcttggca tttgccaaga
     3481 ggattttttt attttaaagg aggaaaagaa gatggagtat ttgattgaag ttatgccgca
     3541 attattagta ggagcactca caacattgaa attattttta tttaccttga ttggctcatt
     3601 accattaggg attgttttag cattaggatt gtctttgcat tttaagccgc tacgctactt
     3661 gcttcaaaca tatgtatggg ttatgcgggg aacaccgctg ttgttgcaac tgatttttat
     3721 tttttatggg ttgccgacga ttgggatcgt ttttgatcgt ttcgaagcag cactcattgc
     3781 ctttatctta aactatgcag cttattttgc agagatcttt cgtggaggga tacaatcgat
     3841 tcctcaaggt caatatgaag cagcgcaagt tttgcaatta agtccctttc aaacggtgac
     3901 gaaaatcgtc atcccacaag tgacaaagat tgttttacct tctgtaggga atgaagttat
     3961 caacttggtc aaagattctt ccttgatcta tatattaggt ttaggtgacg taatgcgtgc
     4021 tgggaaaatc gcgatggaac gcgacgcgac tttactgcct ttgctaggcg tggcaatcat
     4081 ctatcttaca ttgactggta ttctaacaat cataatgaaa caagttgaag gaaaattaaa
     4141 ttattaccgc taactgatag gaggaagaaa tatgattgaa ttgaagaaag tgaacaaagc
     4201 atttggtgaa aaaaaaataa tcaaagattt gaatctaatc attccagatg ggaaaacatt
     4261 agctatcgta ggtccttccg gtggcggtaa aaccacgctt ttacgtatct tggctggctt
     4321 agaaagttct gacagcggag aattttattt agacggtgta ccgttcaacc cattcgagct
     4381 taaaatgaaa gaacaagttg taggagtcgt attccaagac ttccagctat ttccacatct
     4441 gaatattttt gaaaacatta ccttagcacc aaaaatggta ttgaaacaga caaaagaaga
     4501 atacaccaaa aaagcagaag aattagcggc ttcattaggg attagtgatt tattgaatat
     4561 ctatccttat cagctttctg gcggacaaaa acagcgggta gctattgcca gagcattggc
     4621 tatgaatcca cgggtattgg cttatgatga accaacaagt gctttagatc ctgaattacg
     4681 ccagcaagtc gagcgtttga tcctatcatt gaaagaagat ggggtaaccc aaattattgt
     4741 cacccatgat ttagtatttg cagaaaatgt cggtgatcaa ttactaaaag tttccccaat
     4801 tcattaagaa aggagaagat agagatgaaa aaaaccagtt ttctattcgc tttgatcgca
     4861 ggtcttcttc tttttgcagg ctgttcgaac aaaaaaactt cagcagataa ttgggcgact
     4921 atcaacgaag agaaaaaaat cgttataggt ttagacgata catttgttcc tatgggattt
     4981 agagataaag acgggaagct cacaggattt gatattgatt tagccaaagc agtctttgaa
     5041 caatatggga ttacggtaga ctttcagccc attgactggt caatgaagga atttgaactg
     5101 aacaatggaa cgatcgattt gatatggaac ggctattcca aaacgccggc aagagaaaag
     5161 aaagtgcaat ttacaaaacc ttatatggaa aacgatcaag tgctgatcac acctaaagaa
     5221 tcaggaatca caaactttgg acaaatgaat aataagagat taggtgcgca aaatgggtca
     5281 tctggttatg atgtatttac gaaacaacca gaagtattaa aagagatagt caaaaaccaa
     5341 gatgctgttt tgtatgatag ttttaatgaa gcattgatcg atctgaaaag cagacgggtc
     5401 gatggtctgc tgatggataa agtatacgca gactattatt tgaaacaaag aaacgaacta
     5461 gaagacttca acataacaaa aggttcttat gagagtgagg attttgcagt tggtgcacgc
     5521 aagtcagata gtacattagt gaaaaaaatc aatgaagccc tagatcaact agagaaaaca
     5581 ggtgaattcc aaaaaatatc cgataaatgg tttggcgaag atgttacgcc gagaaattaa
     5641 caaatagggg actgtgacaa aagacgtgtc acagtccctt tttccgaata aacagtgttc
     5701 tcctgccaac tccttgtgac tcggagctga atggcaggct cacaacctct tggtaaacgg
     5761 tgttcaaaag ccagtttttc ggtattaagc caaatttatg caaaaatgta gaaagccatt
     5821 ttcacaaaat ttttcttact actcgaaact aagcgacttt ttcacaacct cctttacata
     5881 attttatttt gttgtggaag gggtttcgtg ttattctaaa agaggaacga ttgaaggtta
     5941 taggaaagga agagatgaat ggaacacaaa gaacttacct catttccaaa agattttctg
     6001 tggggctctg cttctgcagc gtatcaagta gaaggagctt ggcaagaaga tgggaaagga
     6061 gaatcagtct gggatcgatt tgtacggata cctggaaaga catttaaagg aacgaacggt
     6121 gatttggcag tagaccacta tcatcgttat aaagaagata ttgctttaat gaaagaacaa
     6181 ggtctgaaag cttaccgctt ctctgtagct tggacacgga tttttcctaa tggacgagga
     6241 gaagtcaatc aagctgggct cgctttttat gagagattga tcgacgaatt gattgaaaac
     6301 gatattgaac ctatgttgac tttatatcat tgggatcttc cacaagcatt gcaagatgaa
     6361 tataatggtt gggaatcacg tcagatcatt gaagatttca ctcattatgc agaaacatta
     6421 tttgaggcat tccgcgggaa agtccattac tggattagtt tgaatgaaca aaatatcttc
     6481 acttcattag gttatttgtt agcagcacat ccgccaggtg tgacagaccc gaaacgaatg
     6541 tatgaagtta accatattgc taatttagca aatgcatctg tcatcaataa atttcacgag
     6601 ttagaaatgc ctggaaaaat cggaccaagc tttgcgtata cgccaaatta tccaattgac
     6661 agtaatcctg aaaatgttct ggctgctgaa aatgcagaag acctgatggc caattattgg
     6721 atggatgtct atatgtgggg aaaatatccg attgctgcca tgaggttctt agaagaaaaa
     6781 ggatgggcac caacgattga agctggcgat gcagaattgc tagaatctgc taaaccagat
     6841 tttcttggga tcaattacta tcaaacggcg acgaatgcat tcaatccatt aaacggagtt
     6901 ggtgctggaa aaatgaatac gacaggaaaa aaaggttcat cagaagaaac tggagttcct
     6961 ggaatgtata aaaaagttga gaatccattt gtcgaacgaa caaattggga ctgggagatt
     7021 gacccagaag gattaaggat cggactgcgt cgtatcacaa gccgttaccg catgccggta
     7081 ctgattacgg aaaatggctt aggagagtat gacaagttga cagaagataa acaaatccat
     7141 gacgactatc gaatcaatta cttacaaagc cacgtcaaag ccatcaaaga agcaatttcg
     7201 gatggagcag aagtcctagg ctattgcacc tggtcttata cagatttgct tagctggctg
     7261 aacggttatc aaaagcgata tggatttgta tatgttgatc aagacgaaac acaagaagga
     7321 tctttggaac gatataagaa agacagttat tactggtacc aaaaactgat tgaagaaaat
     7381 ggagagaacc tttagttcac atacttaaaa caagaaggta cggctggata tggccgtgcc
     7441 ttttcttgat aaaatcattt agaaattaag aaaaactgta aaataagctg aaaaatgaaa
     7501 gaaagcgtca gttctttcaa acaaaacttt agttccctta cttttttttg tataatgaaa
     7561 cttgatgaaa tgagggataa taaatggcac aaaagaaacg gcctactaaa aaaaggaaaa
     7621 agacgaaaaa aacgaaaaag caaaaacagc agcaggaaca aatgctgacg attggtgttg
     7681 ggtttgcctt gattttattt gctgtcttcg gctttttaaa attaggtttt ttaggaatat
     7741 tgattgcaaa cggctttcgg atcattgcag gaaatactta ccaaatcctc tgtcttttat
     7801 tagccgtatt aggattttgg attgtcatta agaatacaga gttttcaatt ggtaaaaacc
     7861 gacgctggtt tggcggcata ctcttttatt tcggtatctt attattgtta catgcccatt
     7921 tatttggcaa actccatact ggggaaccaa atatcatggg gacgacatgg gatttgttag
     7981 ccagtgatat aaaacaaagt caagtagaca ataacgttgg tggtgggatg attggagcaa
     8041 tcctttatca ttttacttac ttcttgattg cacagcctgg aagttattta gcagcagtgc
     8101 tattactggc aggaggagca ttcctgttca gtaatctgga aggctatcag ctgttaaatg
     8161 gtatccaatc aattggagaa cgagtacagg aattattgga aggtgatcct gccaagcaag
     8221 ctcgaaagca agcggcaaag gaagagagaa tgaaacagcg cgcagaagcg aaagaagcaa
     8281 ggcgcttagc tgcaaaagaa gctgcagaaa aagaagctgc agaatatgag aagaaaaaag
     8341 cggtgagcca gcaaagggaa aatactgctg atgaatggca ggaaccagaa aatacagagc
     8401 cggagcagct aagttttgtt ccaatcgaca gttttcaaga aaatatccat cctgcgaatc
     8461 tagagaaacc agtaccagat acaccaaaac agaccaatac agcagaaggt ttttcagatg
     8521 agttgccaga ggatgacggt acctcattag aatttgagat tgaagcagaa caggaaaatc
     8581 aagactatga attaccgaca gttgatttgc ttgattctat accaactgtg gatcaaagtg
     8641 atgagtataa aaaaattgaa aaaaatatcg gcgtattaga acaaactttc caaagtttcg
     8701 gtgtcgatgc caaggtcgtc aaagccagct taggaccatc cgtcacaaaa ttcgaagtcc
     8761 agccggctgt aggggtaaaa gtaagtaaaa ttgtgaactt aacagatgat atcgcgcttg
     8821 ctttagcggc gaaagatgtc cggatggaag caccgattcc aggtaaatca ctgattggta
     8881 ttgaagtacc aaatggaaag atcagtatgg tttcctttag agaaatcatc gaagcacaac
     8941 caaaccaccc agataaatta ctagaagtac ctttaggacg tgatgtttct ggacgggtac
     9001 aaacggctga tctttcgaag atgcctcact tactcgtggc tggttcgact ggtagtggga
     9061 agtcagtagc aattaatggg attatcacaa gtatattaat gcgtgctaag ccccatgaag
     9121 taaaactgat gatgatcgat ccgaaaatgg ttgaattgaa tatgtacaac ggcatacctc
     9181 atctgttgac gccggtcgtg acgaacccta gaaaagcagc gcaagcctta caaaaagtag
     9241 tacaagaaat ggaagaacga tatgaaaaat tcgcggcaac cggagtaaga aatatttctg
     9301 gttacaatga atttgttcaa cagaaaaact tggaaaacgg aacgaaacat ccaaccttac
     9361 cattcatcgt agtgatcgta gatgaattag ctgatttgat gatggtagcc agcaatgaag
     9421 ttgaagatgc gattattcgt ttagcgcaaa tggctcgggc agcggggatt catatgatcc
     9481 tagctactca acgtccaagt gtggatgtta tcacaggaat catcaaagca aatgtacctt
     9541 ccagaatggc atttgcggta tcaagcggta cagattctcg gacgatcatt gattctaatg
     9601 gggcagaaaa gctcttaggg cgaggagata tgctattctt gccaatgggt gagaataaac
     9661 cgatccgtgt gcaaggagca ttcatttctg accacgaagt agaacgggtc gttcaatttg
     9721 ttacagatca gcaagaagct cattatgaag agaaaatgat gccgacagat gaagttgaaa
     9781 cgacaggtgc tccagaacag ccacaagatg agctgtttga agaagcaaaa gcacttgtcg
     9841 ttgaaatgca aacggcaagt atttcccttc tccaaagaag atttcggatc ggttacaacc
     9901 gagcagcacg tttagtagat gaactggaag ctcatggagt tgttggcccg tcagaaggaa
     9961 gcaagcctcg gaaagtattc attgagcagg aagaaacatc agaaacgttg ccggacgata
    10021 atgacatgta aaacttgtgt gatgaagggt tgaaaaacga ataaccaata aaaagacaag
    10081 gcatgatgat accaaaagaa aaaatggttt ttttccatct tttctaatac ggtatgatgt
    10141 cttgtctttt tagtttttgt gagattaaag ttctattcga tttatttgtg aagtattgca
    10201 ttaaggaaat gaatcatgta tagtatgtag cgaaactttg agaaaaagag ggaaaatact
    10261 ttggaaaaac agtccattta tggcttgacg aatgaagaat taatcaattg gtttatagaa
    10321 aacggcgaga aaaaattccg agctgcccaa gtttgggaat ggttgtatca aaaacgtgta
    10381 agtaatttca cagaaatgac gaatctatcg aaacaattga tcgaaaaatt atcggagcat
    10441 tttatcatca atcctctaaa acagatggtc gtccaagaag cttcggatgg tacagtaaaa
    10501 tatttgtttg aactccctga taaaaacatg attgagactg tactgatgag acaagaatat
    10561 ggcttgtcag tttgtgtgac gacacaagta ggatgtaata tcggctgtac gttttgtgca
    10621 agcgggctgc tcaaaaagaa ccgtgatctg actgctggag aaattgttgc acagatcatg
    10681 atggtccaac attattttga tgagcgcaat cttggcgaac gcgtttcaca tgtcgttgta
    10741 atggggatcg gtgagccatt tgataattac gacaatgtaa tggacttttt gcatatcatc
    10801 aatgatgcca aaggattggc aattggtgct cggcacatta cagtttccac tagtggatta
    10861 gcacacaaaa tcaaggaatt cgctaataac ggcttgcaag tcaatctggc tatttccttg
    10921 cacgcgccga ataatgaggt gcgaacatcg atcatgcgga tcaaccgaag tttcccaatt
    10981 gagaaattga tggaagctgt ggatgagtat ttagagaaaa caaaccgtcg aatcacgttt
    11041 gaatatatca tgttgaatca agtaaatgat agaccagagc atgcgcaaca attagcagat
    11101 ctattaaaag ataagaaaaa attagcatat gtcaacttga ttccatataa tccagtcagt
    11161 gaacatgatc aatattctcg cagtccaaaa gctgatgtat tgagatttta tgatgtattg
    11221 aagaaaaacg gcgtaaactg tgtgatacgt aaagaacatg gtacagatat tgatgcagct
    11281 tgcggacaat tgcgaagcaa gcaaatgaaa aaaacagttg ccaaataatc gtatcctact
    11341 cgttttttca attaccataa aaggagcagt tttcttaatt aaggaactgt tcaacttaaa
    11401 taagagaaaa gattctatag cgaaagctat aaagcttttt ttgacaagaa aaaccctcat
    11461 caagcaaaga aatgtcctca atttgatgac agcctgcttg atgggggttt tagcaattat
    11521 ttaactgatt ttcttatatc cttgctggct ttcttgtgta gcatgataag tttctacgtc
    11581 aagaatggcc tgtttaaatt tttctcgggt gatttcataa ggcacatgtt taatatcatt
    11641 tgttgccatg gctttatcta agattgcttc taaatcggtc gtgacattga gattcatgtc
    11701 ggttaacgat actggaagtt ggttcttttt cataaacaca tagattttat caaacaaatc
    11761 gaactgctga tccatcatca ataaggtcag tacaccataa ctgacagatg ctccgtgaag
    11821 gtatccctcc attttttcta gaacagtcgt accgtaatag aatgagtgtg ccatagcaga
    11881 attatagtta ttgttaacta aaacagaaac caatccggta gtaacaatga tatccgaaac
    11941 gacttgttca attgcatatg agggacgatc gtcggcacta tctttcaatg cttgcgtacc
    12001 ataagtcaag agacgatcaa tgcacatttt tccgatttgg attcctaatc tatccgtgta
    12061 gcctagatca tcgccacgag cagaaaaagt agattcgact tctttggata aagcatcgcc
    12121 aatacctgcc cataagtatt tatcaggtgc ttcggcaata attgctgtat caatgaaggt
    12181 gtgtacaggt ggttttgaag gaaagaagag tccgtccatt acgccttctt cgttatagat
    12241 gacacatacc gcagtgacgg gcgaacagtt cgaaccaata gtgggaaagg tgaagactgg
    12301 tttagctagt ttttcaccaa tgacttttac agtatcgcaa gcacggccac caccgactgc
    12361 aaacaggacg tctgcttttt ggacactttc atgctggatc aactgttcaa tatttccata
    12421 agttgaatta cccccgtacc agagactggc gacaacctct atttcctgtg tcaaagctac
    12481 ttttattttg ttttctgcct ttgaaagtgc agtcttccct ccaatcagca ctacttttgt
    12541 cccatattct ttgacgattc catttgcttt ttcatacgca tctgttccaa tggtataatt
    12601 tggtaagaaa acactttgac tcatattcat ttccttcttt cttcgttgaa acgggttaaa
    12661 attttaaaga gtgatcgatc tgactgcggc tgtagatgga gtagaggtga acctaagaca
    12721 ggataagtga tgaataaggt ggcatgaaca ccttgaaatc atcaataagt atcctaaaaa
    12781 cgaacaagta caaaaacatt gaaaacgaca ataatgaatt tttgttcctc ttagtatttc
    12841 taccgattcc ctttgctttt ttgtaattat
//
LOCUS       DAZKLA010000074        12854 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000074 DAZKLA010000000
VERSION     DAZKLA010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.075"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..731
                     /locus_tag="ACG85N_001833"
     CDS             <1..731
                     /locus_tag="ACG85N_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0224597.1"
                     /translation="SILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNV
                     ALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSY
                     GGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINK
                     GEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLL
                     GSIQTNLPESVLLDCGMDFLKDIC"
     gene            789..2828
                     /locus_tag="ACG85N_001834"
     CDS             789..2828
                     /locus_tag="ACG85N_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0224598.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2937..4088)
                     /locus_tag="ACG85N_001835"
     CDS             complement(2937..4088)
                     /locus_tag="ACG85N_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0224599.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4242..4772)
                     /locus_tag="ACG85N_001836"
     CDS             complement(4242..4772)
                     /locus_tag="ACG85N_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0224600.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4871..5221)
                     /locus_tag="ACG85N_001837"
     CDS             complement(4871..5221)
                     /locus_tag="ACG85N_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFQ0224601.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5378..6907)
                     /gene="ahpF"
                     /locus_tag="ACG85N_001838"
     CDS             complement(5378..6907)
                     /gene="ahpF"
                     /locus_tag="ACG85N_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFQ0224602.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6917..7480)
                     /gene="ahpC"
                     /locus_tag="ACG85N_001839"
     CDS             complement(6917..7480)
                     /gene="ahpC"
                     /locus_tag="ACG85N_001839"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFQ0224603.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7624..8286)
                     /locus_tag="ACG85N_001840"
     CDS             complement(7624..8286)
                     /locus_tag="ACG85N_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0224604.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8470..10116
                     /locus_tag="ACG85N_001841"
     CDS             8470..10116
                     /locus_tag="ACG85N_001841"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0224605.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10107..11189
                     /gene="uxuA"
                     /locus_tag="ACG85N_001842"
     CDS             10107..11189
                     /gene="uxuA"
                     /locus_tag="ACG85N_001842"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0224606.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11297..12850)
                     /locus_tag="ACG85N_001843"
     CDS             complement(11297..12850)
                     /locus_tag="ACG85N_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0224607.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
BASE COUNT         4303 a         2360 c         2382 g         3809 t
ORIGIN      
        1 tatctatttt gttactgctt acattagtag ttggctattt ttcgattgag tttatccatg
       61 gattttcgtc tgcaaaacag agttcaactg tgaaaaaggt agagccgaaa agtatcccta
      121 ccacactaaa tgtggctttg attggttcag atgctcgttc gaaagacgaa aacggtcgct
      181 cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa ctgatctcta
      241 tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata aatgcagctt
      301 actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc aaatttgaag
      361 ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat gaactgtttc
      421 ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta tatataaaca
      481 aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt cgggaagaca
      541 aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct atctctcagc
      601 agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa ttacttggta
      661 gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat ttcctcaaag
      721 acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag caagagaagg
      781 aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct tatattgccg
      841 gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag cactatcaag
      901 aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa gctttgaaca
      961 agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat
     1021 tatctgaaaa agagatctta gataaatacc agaatatata cggtgctgct gatgtcaaag
     1081 gacttcagat atcgaatcta aaagtagata aaaaagacga ttctacttac agtttttcat
     1141 ataaagcaaa gatgaatacc tcattaggtg aattgaaaga tctttcttat aaaggaacat
     1201 tagacagaaa tgatggtcaa accacgatca actggcaacc caacttggtt tttccagaaa
     1261 tggaaggaaa tgacaaagta agcctgacca cacaagaagc agcaagaggg aatattatag
     1321 atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa acaattagga gtcgttccaa
     1381 gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa agcgattgct tcctccttcg
     1441 acttaacaga agatgctatc aatcaggcga tttcacaaag ctgggtacaa cccgattact
     1501 ttgtcccatt gaaaatcatt gatggagcaa cgccagaact tccagctgga gctaccattc
     1561 aagaagtaga cggcagatat tatcctttgg gtgaagcagc tgctcagctg attggttacg
     1621 tgggagatat cacagcagaa gatattgata agaatccaga attaagcagt aatggaaaaa
     1681 tcggtcgctc aggtttggag atggcttttg ataaggatct tcgtgggaca acaggtggga
     1741 aattaagcat tacagatgca gacggtgtag agaaaaaagt tctgatcgaa catgaagttc
     1801 aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc acaaaaaaca gcttttgaca
     1861 gtctaggagg aaaagctgga tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg
     1921 cgcttgctag ctctccaagc tatgatccaa acaaaatgac aaacgggatc tcacaagaag
     1981 attacaaagc ttatgaagaa aatcctgaac atccattcat cagccgattt gcgacaggtt
     2041 atgctcctgg atctacgttt aaaataatta cagcagcaat cggtctcgac aacggcacta
     2101 tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac agttcttggg
     2161 gatcttttca aacaacgcgt gtcagtagtg atgtatcaca agtagactta aaaactgctt
     2221 tgatctattc cgataatata tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat
     2281 ttcgtacagg cttagataaa ttcatttttg gtgaagacct tgatttgcca attagtatga
     2341 atccagcaca aatttctaat gaagatagct ttaactcaga tatcttgcta gctgatactg
     2401 gatatggaca gggcgaactt ctaattaatc ctatccagca agcagcaatg tattctgttt
     2461 ttaccaacaa tggcacactt gtctatccta aattgattgc agataaagag acaaaagata
     2521 agaagaatgt catcagcgaa acagcagtac aaacgatcgt gccagatctg agagaagttg
     2581 tgcaagatgt aaatggtaca gcacattctc tttctgcttt agggattccg ttggcagcga
     2641 aaactggtac agcggaaatc aaagaaaaac aggatgtaaa ggggcaagag aacagtttct
     2701 tgtttgcttt caatcttgat aaccaaggtt atatgatggt cagcatgttg gaaaataaag
     2761 aagatgatga ttcagcaacc aaacgagcat cggaactatt gcaatacctc aaccaaaatt
     2821 atcaataaaa aattgctttt taagtacagt aaaaggactg tggcgttatc gtcacagtcc
     2881 ttttttcaaa taaacggttt tcacgatgtc tctttcacaa cttctttttg atttactcac
     2941 acttcataaa ctttttttgt aaaatatttg agaacaagtg gagaaataag tgtacttaat
     3001 aaaatcacga taactaaagg tgaatagtac tgtggttcga tcaattgact ttgctgtcca
     3061 atttgtaaaa tgatcaaagc catttctcca cgcgaaatca taccagcacc gaccatcaat
     3121 gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat aacctccaag caatttggtc
     3181 aaaatcgcta caagcgtcaa tatagaaata aagagtagtt gttcactaaa gtcagaaaaa
     3241 tcaacttcta atcctacact gacaaaaaat actggtataa atactgcata tcctaaagcc
     3301 tctacattgt ggtatacttc ttctttaacc tttgtctgac tgacagctat cccagcaaaa
     3361 aaagcaccaa tgacagagct aagcccaaca agatccgcta cgtaagacat tcctaaacaa
     3421 ataactagcg atacgatgat aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt
     3481 aggaaaggcg caatccattt gactaataag aaaataaaaa caaaataaat tagctgttca
     3541 atcaaaacaa gcggtaaaga agcattgcta gaagcttccc ccgttagaaa agataggctg
     3601 aaacttagaa ctaaaacgac taaaatgtca tctaccacag aagcacccaa aattgttgat
     3661 ccttcttttg tattcactac gtttagttct tttaatactt ctactgaaat actgacagaa
     3721 gttgccgcaa gaataatccc aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg
     3781 cctgtcaacc agccaaaaac aaccgggaag atgatcccaa gcaaagcaac gaacatacca
     3841 ggtcggaaat attttttcaa aagagataaa tcactttcca gaccagctaa aaacattaat
     3901 aagatcacac caatctccga aaagtcatgg actagaatat caggtttgac ccaattcaat
     3961 ccagcttggc ctagcaaaac accaacaagt aattgtccaa tcactgctgg gatacctaat
     4021 ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata tcaggcataa aataccaata
     4081 aattccattc tcatcattcc tttgtataaa atatagcccg aacttagcat aacatacatt
     4141 attgaaaaaa taggaaaata tactcacaaa aaaaccaaca atccaaaaga tttttttgga
     4201 ttgttggttt ttatacggtc tattttaata actaaaaatg attaacgtat agtcattttt
     4261 cttattctga tgaaattgat aatctcctgg atgcccatga aaatcaaaat aaagccgaat
     4321 atttggaata gaagcatcaa acttttgaat ggattgaaca ataagacgaa tccgccaatc
     4381 aacaacagaa tactactata aacactccaa cttttagctt ttgtattcat gctgaagaac
     4441 aactggaaca agctgttgat gataacagct aatccaagaa gaatcggcag gatagaaacg
     4501 attgcaggtg caaaagcata gacgatcaga gctaagatca ggaaaaaaag accactgaac
     4561 agaccgatac tccaagaacc tgttttcctt ttaaaacggt aagcctcaaa aagattcaac
     4621 aacccaagca aaacaagata ggcggtgatc agataaccga taaaatgaaa aacagcagaa
     4681 gggtttagca caatcgcaat cccagaaata atataaacag cagctcttaa taaggcgtat
     4741 ttttgaatat tttgtttaat cgtctcaaac atataagaaa acctcctttg acttatttta
     4801 acgtgaacta gtcattttac caaattaaat gttacaaaat gagcgaagga gaagaatatc
     4861 tctggaaaaa tcaacttctg aggatctttt ccattttttt gccttttgct agctcatcga
     4921 tcattttatc taaataacga atttctttca tcaagttatc ctcgatgtct tctactcgta
     4981 caccacaaac aacacctgta atcaaggaac gtgaaggatt ggggacaggg gcttccgcaa
     5041 aaaattgttc gaaagtcact tgtttttgta gttgatattc tagttctgtt tgggaataac
     5101 ccgtcagcca acaaatgatt tcatctactt cctcttttat acgatctttt ctctccgctt
     5161 ttgctacata cataggataa acgcttgcaa aacttgtcgt aaaaatacga ttttgcgcca
     5221 taatcagtca cctttctttt tttcaaatta tttactactt atagtatata agaaaaagag
     5281 aagaaaaagc gattattcaa aaaaacaaaa aggattgtga caaaattctt gtcaccatcc
     5341 ttttttccgc tctctgactt tttacaatct tcatagctca agtgcgcatc agataatcga
     5401 atgctcctaa agctgcagta gctcctgaac ccattgaaac aatgatttgt ttgtatggac
     5461 tatctgtaca atcaccagct gcgaaaacgc cggggatatt cgttgcgcca tttttctcgg
     5521 tcactatttc atttcttgga ttcatttcga tcgttccatc tagccaatcc gtattaggga
     5581 gtaagccgac tagaatgaat actcctgaaa cagctagtgt atgcatctcc atcgtacggc
     5641 gatcgatata ggagagagat tctacctgat tcgtaccgct gatttctttc gtcgctgcat
     5701 gtttgataac agtgacattc gcaagagaga ctagtttatc ctgtaagatt tgatctgctt
     5761 ttaattctgg taagaattct agaacatata catgttttgc taacccagcc aaatcgattg
     5821 ctgcttcaac accagaatta ccaccaccga taacagcaat ctcttttcct ttaaataaag
     5881 ggccgtcaca gtgtgggcaa taagcaattc ctttattttt aaactctttt tcgcctggta
     5941 cattgataga acgccagtgc gcacctgtcg cgatgatggc ggtttttgct ttaagtgata
     6001 taccgttagc taagccaata ttaataaaat gttgctcttt atttaaggaa acggcctgtt
     6061 gattcatcat aatgtcgata gggtaactac gcacatgact ttctacttgc tgcatcaatt
     6121 ttggaccttc tgtgtaagag gtaccaatca tattttcaat ccctagtgtt tcaacgactt
     6181 gtccgccgaa agaatcagcg acaagtcctg tacggattcc tttacgtgct gcatagatag
     6241 ctgcactact agctgctggt ccaccgccaa ttactaatac atcataaggt tctttttcaa
     6301 acaactcttc ttcaatcaaa gggccagtga ttttttcaac aatttgttcg atcgtcatgc
     6361 gtccgctact aaattcttct ccatttaaaa acacagttgg cactgccatg atttttttat
     6421 catcgatttc tttttggaac atcccacctt caatcatcgt atgagtaata tttggattta
     6481 atacagctaa tatattcaat gcctgtacaa cgtcggggca attatgacaa gtcaagctaa
     6541 cataactgtc aaaatgcaat gtttgtgtta tttttttgat tttaagactc aaagattcac
     6601 tgattttcgg tgcccgtcca ctaatttgta acatagccag taaaaaagag gaaaattcat
     6661 gaccgagggg gataccagca aaaacgattc cactttcttt gccaactgga ttgatagaaa
     6721 aactaggtgt gcgcatcgaa tctctttttt cccaagagat tttaggagac atttctgcta
     6781 tttccttcgt aaattcagct agtttttgac tattttcact attatccaaa ctaacagaaa
     6841 aaacaatagg agattcaatc agtgctaaat attgttcgag attttgttta gtttctttgt
     6901 ctaacatatc caccacctaa atctttccaa caagatcaaa acttggtttc aatgtttcgc
     6961 cactttcttt ccattttgcc ggacaaactt cacctgggtg ggtacgtaca tattgagcag
     7021 cgcgaatctt atcaatcagt gcactagcgt cacgaccgat tccatctgca ttgatttcca
     7081 tagcttgaac gattccgtct ggatcaataa taaatgttcc tcgctgagct aatccttgct
     7141 cttcatccaa aacatcaaaa atacgagaaa tctgatgaga cggatcaccg atcatcacgt
     7201 attcgatttt accgatagca tctgatgtat catgccatgc tttgtgagta aagtgggtat
     7261 cggtagagca agaaaagact tcaacaccta aagatttcaa tgtactgtat tgttcttgta
     7321 agtcttctaa ttcagtagga caaacaaaag taaaatcagc tggatagaag caaagaatac
     7381 tccagttccc cataagatct ttttcactga cttctaaaaa ttctccttga tgataagcat
     7441 gtgcattaaa ttcttcgatt tttttcccaa tcaatgacat agtaaaacct ccaataataa
     7501 ttatttattt ataattattg taatttagtt ggagttgcaa agtcaatgga tattttgtta
     7561 tttaataaag attactcaaa aacgaaggtt aattataaaa atagaagagg tcgcaagaaa
     7621 atgctactgc tggaaatact cgggataacg ttgagtaaca gtttcctgtt ctttcagcat
     7681 catgtgtaag tgcaatcccg tttgaaatcg agcttcttct ggttttttat caactaaagc
     7741 cttgagtaaa tcataatgtt gttccatgat cgtttcccat ggaagcccct gaatggtcac
     7801 tcgtaaccag cggaaacgtt ctaaatgggt attatggtca tctaaccagt tccaaacttc
     7861 ttttcttcct gcaatttcga aacaagcttc atgaaataaa ttatctgcac ggaaaaaagc
     7921 gcgttcgtca tttattttga ctgctttttc ttgttcttct aaaatcgttt ctagcactat
     7981 tcgtgtttgt gtgtccaatt tggaacaaca ttcaatcatg atttgctctt ccaactgttc
     8041 tcggacaaag cgagcatttt ctgcagaaac aagattaatt ttggaaacat acgtaccact
     8101 ttgaggtatc gtatacacga gttcttgctg acgtagttga atcaatgctt cgcgaatggg
     8161 agtccggccg atagcaagat gttcctccag tgccttttca gatattttcc ttccaggttc
     8221 taagtcagca tagataattt gtttacgaat cgcttgataa gcttgtgatt gcaagctgct
     8281 tttcatcttt attcctccaa aatcatttct atgaacgtag tataacagaa gaggtctgaa
     8341 aaacaatctg gtatgtcaca ttaactagta aatttgtata aatagtaaaa ttttggttga
     8401 catagcgatt tttattgtat aaaattcact tgaatacaaa aactaatatg ttagtttagg
     8461 aggggaaaaa tgattgattt aaacaactgg aggaacttta tccctgaatt atctcaaaaa
     8521 gaagtagaaa tacctcattt tgaccgtgca tcattaagag aaaatggaca aaaaaatcct
     8581 gtctggatcc atttcggtgg cggaaatctt tatcgaggat ttcatgcaga aatcgctcaa
     8641 cgtttagcgg atctaggcga attgtccact ggtgtcattg tttgtgaaac attcgatgaa
     8701 gaaataattg aaagggctta ccaagatttt gatgatttga ttcttgaagt ggtcatgcca
     8761 agcagtggag agttcgtcaa acgtatatta gctgcgacag ctgacagtct gtattgtcat
     8821 ccagattgtt ctgaacactt tgataagatg attcgacact ttgaaaatcc tagtttacaa
     8881 ttagttactt ttgctattac tgaaaaaggt tatcgattga aagatacaaa taataaatat
     8941 ataggaccga ttgcagtcga tataaaaaat ggtccaaaga agccatcaca tacaatgagt
     9001 attcttgctg cgctacttta taaacgtttt ttatctggag cttggccgat tgcacttgtc
     9061 tctacagata atttttcaca aaacggtagt caattacgta cttctttgct aacgatcgca
     9121 aaagaatggc aaaaggaagg ttatgtacaa aatacattta tccaatactt atcgaatgaa
     9181 gaaattgttg cgtttccatg gacgatgatt gatcgtatca ccccgaatcc ttccttgtca
     9241 gttagcaaaa tgttggcaaa agagggcttt tcaaatatgt cgattttcca aaccgcaaaa
     9301 ggaaataata ttgctccatt tgtcaataca gaggacatcc attatttaat catggaagat
     9361 cattttccca atggacgtcc gaaattcgaa aaagctgggg tattcttgac tgatcgtgca
     9421 acggtagata aagcagatac gatgaaggtg acgacttgcc ttaatccttt gcatacgtct
     9481 ttagctatta tgggttgttt actggggtat aggagcatag ctgaggagat gaaagatcca
     9541 gatttatcgc tgctagtttc tgaaatcggg tacaatgaag gcctcccagt tgtggagtct
     9601 ccaggtatca ttgatccaaa gatctttctg gaagaagtaa ttactgtgcg cttttctaat
     9661 ccaaatattc ctgatatgcc tcagcgaatc gccacggata cctctcaaaa ggtggcgatt
     9721 cgttatggtg agacgattaa aaaatatatg gaaatagaag ttaaagaccc gcagacactt
     9781 gtattcatac cattgactct cgctgcttgg cttcgatacc ttattggcat tgatgatcaa
     9841 ggaaaaccat tcatcccgag tccagaccca ctattgacag actttcagca taagctttcc
     9901 gatattcaac tgggtacgca agatacatca catattcatg agaaaatggc tcctattttg
     9961 tcaaataaag aaatttttgg tgtagatctt taccaagcag agcttggcag aaaaatagaa
    10021 gaaatgttca tcaagatggt agcaggaccg aatgccgtac gaaaaacatt acaggattac
    10081 ttacaagaac atggaggaaa tttatcatgg aaatgacttt tagatggtac ggatcacagg
    10141 aagaaaagat taccttagat gaaatcagac agattcctgc ggtcaaaggg attgtaggca
    10201 cattgatgga tataccagta ggagaggtat ggcccaggga gaggatcaaa gaactaaaag
    10261 aagaaattga gtctcgtggg ttaacactaa aagtaattga aagcgtcaat gtgcatgatg
    10321 ccattaaaat tggattacct tcccgtgagc agtatatcga aaactataag cagacaatcc
    10381 gaaacttggc agaatatgga atagaagtca tctgctataa ttttatgcct gtatttgact
    10441 gggtgaaatc tgaccttgac taccgcttag aagacggctc atccacgtta gcttttatat
    10501 ctgatgatat tccagatgat ccaagagaaa tcgtcgcgcg catcgaacat tccagcaatg
    10561 aatttgaatt gccgggatgg gaacctgagc gcttagctca tatcaaatcc ttgcttgaag
    10621 cctatgcatc agtagacgaa gaaaaacttc gagaaaattt tgcctatttt ctccaatcaa
    10681 tcatcccaac atgtgaagag gtaggggtca aaatggctgt acatccagat gatccgccat
    10741 actcgatttt tggattacct cgaatcgtaa agaatcgaga agatttagat tggttgtgcc
    10801 gcatagtaga cagcccttat aacggaatca ctctttgtac agggagtatt gcagaagatc
    10861 cagaaaatga tgtatatggg attttagcag aattttgtca aagagatcga atcccatttg
    10921 cacatgttcg aaatattaaa tttttagcta atcgagattt ctatgaggca ccgcataaat
    10981 ccgaatacgg ttcattagac atgtaccgca tactaaaaac aatgtatgac aacggattta
    11041 acgaatacat ccgtcctgat catggaagaa tgatctggaa tgagacagga agaccaggat
    11101 acggcttata tgatcgagca ttaggtgctg tttatctaaa tggattatgg gaagcaatcg
    11161 aaaaaggaaa caaaagtaaa gataattagt gaaaaaaact gtaattttat tatgtaaata
    11221 agaaggatgt tcaaattctt gatacataaa tccggaggtt gttaataagc attcggattt
    11281 atgtatctta tttggtctag gaagtaactt gattttcttt tttcgattga tccatgagtg
    11341 cttcttcttt taattcaaag atatattctg agatcctcat aatcactttt ttccagtctt
    11401 ctggtgtgat gattcctttt aaaatataga tttttttgat atgagaaggc aattgtttca
    11461 aagggcagtc gctgataacg atgttgcagt tctccataaa atcattttgt attttgatcg
    11521 attcatttga aaaaatttgt tctatcttaa cgatcagatc tttactgata taaaaatcac
    11581 gcgtatagtt cacaccaatc acaattcgcg gctgtttttt gtagcgatca tacaaatgta
    11641 ctaggtcttc aaccatccat tgcacatttt ccttttctaa aataactggt aacaagtgtt
    11701 gattttgttc tctgaaatcc atataaaatt gttcaatggc ggtttggact tctttcaaca
    11761 cgctatgttc ttgataaagc atgaaagtag gaaggggcgt atttggttga ttgaagttaa
    11821 gaaatccatt ataaagttta tttcgtgcga atgccaatga gtaaagaatc ttttcttttt
    11881 caggtataaa caaatcaaat acagtacaaa aggactccac catttctctt gaataaagaa
    11941 catgaggaac gtttgctttt tttaaaagtt tgtattgctc caagcttgtt tcttccgatt
    12001 cgattttcgt gatagaaagt cgagctaaaa tattcactaa gatgcgttgt tcgcgacaaa
    12061 ttggcagatt aggtcgcaaa atatctacag agggatcaat aaaaaagtca atatattcat
    12121 actctgtgaa ttctttttgt acgtctttca cagaagttgc aggaaaattc tctttacata
    12181 tttttaatag aaaatataat ttatctatca taccgctatt tagtcggtca agtacttctt
    12241 ttttaaaaaa agtattcatc ataaaagcca ctacttgcgg atcttcatca taaaattctg
    12301 tagcagtact tgaaaagatg ttccaataaa tatctaatgc tgcatattga atttgtacct
    12361 cttttccaga aaaagacttt ctgccattat tagaaaagga gatagacaaa ccgtacaatg
    12421 ctaaaaatgc attaatcttt ttcattttcg tatacaaata agaggcacta tagtttgttt
    12481 gctgacttaa actgataatc gaatttgtct gacagctgaa caaagaagtc aagatctgat
    12541 aactgactga ttcgttgcag tatcttccaa atagacgata atagcagtaa gccggatcaa
    12601 ttccttcaaa attcaacttt aaaaaaggtt tttgcttatc aatatgcagt tctgttagat
    12661 tgagacgctc aaagtcttgt tctaattctt tgatgtatag taaaaaattt gattcagaca
    12721 tatggaaatg atctgctaaa tattgtgtag taataaagaa acgatgttca gataggaaaa
    12781 gaaaaatatc taattttgta ttgctttttt tcttcaagag aaacgtttta atcgcttctt
    12841 gagtttgcat gaaa
//
LOCUS       DAZKLA010000075        12832 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000075 DAZKLA010000000
VERSION     DAZKLA010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.076"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACG85N_001844"
     CDS             133..3660
                     /locus_tag="ACG85N_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFQ0224608.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACG85N_001845"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACG85N_001845"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFQ0224609.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACG85N_001846"
     CDS             7448..7789
                     /locus_tag="ACG85N_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFQ0224610.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACG85N_001847"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACG85N_001847"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0224611.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACG85N_001848"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACG85N_001848"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFQ0224612.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG85N_001849"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG85N_001849"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFQ0224613.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4344 a         2238 c         2723 g         3527 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aa
//
LOCUS       DAZKLA010000076        12747 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000076 DAZKLA010000000
VERSION     DAZKLA010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.077"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG85N_001850"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG85N_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0224614.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACG85N_001851"
     CDS             complement(323..892)
                     /locus_tag="ACG85N_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFQ0224615.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACG85N_001852"
     CDS             1078..1980
                     /locus_tag="ACG85N_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0224616.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG85N_001853"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG85N_001853"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFQ0224617.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG85N_001854"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG85N_001854"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFQ0224618.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACG85N_001855"
     CDS             complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACG85N_001855"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFQ0224619.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8554..8739
                     /locus_tag="ACG85N_001856"
     CDS             8554..8739
                     /locus_tag="ACG85N_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFQ0224620.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8801..9997)
                     /locus_tag="ACG85N_001857"
     CDS             complement(8801..9997)
                     /locus_tag="ACG85N_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0224621.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10167..11603)
                     /locus_tag="ACG85N_001858"
     CDS             complement(10167..11603)
                     /locus_tag="ACG85N_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0224622.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11896..12705
                     /locus_tag="ACG85N_001859"
     CDS             11896..12705
                     /locus_tag="ACG85N_001859"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0224623.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2675 c         2250 g         4018 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttactcttgc tgtcatccac ccaataagca tctgacagaa
     5101 ctacttttcg ttgcgttttt tcgtgataca taactactgg tatatatcct gggtgtttct
     5161 gaaaaatcac ttgtaattgt ttcattatct cgggatcttc ccgttcttct gtaatacgca
     5221 aatagcaagt ttgtgaagga tactgggctt ctaactggtt aggatcagca aagctttctc
     5281 caattagttg cagttcctga ttatatttgc tgatttcgct ccgcccttga acaaagatca
     5341 ctcgatcaac ggcaaacaaa ttacgatatt tacgataaag agttggaaaa actgtgacag
     5401 aaatttctcc actactgtcg gttccttcaa caaaggccat cagttctcct ttttttgtcc
     5461 gaatttctct aacttccttg atcatcacta ataaattgac tggttgatta gggacgatat
     5521 ctgtaatcag ctgaacttgt ttagctaaac gaagacgatc aaacccttct gcaggatgac
     5581 ctgacaaata aatacctaag tactcctctt ccagcttcaa gcgttcttct agtgtatagt
     5641 cagcgacttc ttcttctttt aatgccatga ttcccagcaa gtcaagactt ccaccactgt
     5701 aaacgatatt ttggattttt ccttcgagat caagcatcaa ttgtttcctg ttaggagata
     5761 attcatcaaa aactcctact gctgtcagtg attgcagtaa ctctaatttc agccaacgct
     5821 tatcgatccg tatgaggaat tgatccactg actggaacgg gccattttgt tttctttcgt
     5881 ttaatatatc ttctacaaaa tcacgccgaa taccttttat cgctgtcagt ccgaatctga
     5941 tttcttcctt attgaccaac tcaaaactgg aattactttg attaatggac ggcgaggaaa
     6001 gtttgagttt atttttacgt gcttctgcaa tatattcttt caattttgat gtgttatgcc
     6061 gaacagaatt catcaggctg gtaaagaaag cacctggatg atgggctttt agatacgcca
     6121 tttggaatcc cacgaatgaa taagcaaatg catgtgaccg attgaatcca tagtttgcaa
     6181 atttttcgat atattcatat acggtcgctg ctgttttttc cgaatatccc tgttgtaatg
     6241 ctcctgatac aaaatgcgac ttttcttcat ctaatacgtc ttttttcttt ttactcactg
     6301 cccgtcgcag aatatctgac tgccctaatg taaatccagc catagtcgag gcgatctgca
     6361 tgatttgttc ctgatagacg atcactccat atgtgttttt caaaatcggg atcagtgaat
     6421 catctgggta gcgaatggct tcttttcctt tcttcctggc aatgaaggta tctatatttt
     6481 gcatagggcc tggtcgatac aatgcattga ctgcggcaat atcttcgatg ttttctggat
     6541 ctaatcttcg taaaacgttc cggataccag cagattcaaa ttgaaatact cctgaggttt
     6601 cgcctcgttg aaacaagagc agcgtttttg catcatttag cgggatctct tttaatcgga
     6661 tctcataccc ttctactcgc ttaacagctt tcaaagtaaa atcaataatc gataagttgc
     6721 gtagtcctaa aaagtccatt ttcaaaagac cgattgcttc tacctcattc atcgtatact
     6781 gggtcagaaa agcttcatca gaaccgcgtt ggagcggaac tagttcaagt agattcttat
     6841 cgctgataac tacacctgct gcgtgagtcg aaacatgtct tggcaatcct tctaaaatca
     6901 ctgcagtttt gaataaaagt tggttttttt cgcttgcctg aactaattct actaattgat
     6961 gcgaagtttt ataagcttgg gtcaatgtga ttttcaattg attgggtatt gcttttgacc
     7021 agcggtttgc ttcactttgc gataaaccaa acactctagc tgcatctcta agaaccattt
     7081 ttgctgccat tgtgccaaat gttgcgattt gtgccatatg atattgtcca tacttttctc
     7141 gaacataatt caatacttct tcacgtcgat tgtcaggaat atccaaatcg atatctggca
     7201 tcgtaaagcg ttcggggttt aaaaagcgtt cgaacagcaa atcatattct aatggatcca
     7261 catctgtgat ttctaacaca taggctacta atgaacccgc agcagaacca cgacctgcac
     7321 cggtaacgat tttgtttcta tgagcaaaag ccattacatc ccatacgatc aagaaataat
     7381 catcgaatcc catttgatgg atgacagata actcgtagtc caaacgttct tggtattcct
     7441 ttgtatagtc agtgattcgg cgttccactt gcgaatagca taactctctt aaatattctc
     7501 cagcttgttt tccattagga actggataat gcggaagtaa tttctgatgg aactggaatt
     7561 caaatgaaat cgattcagct aaacgctggg cgcctattac agcttgggaa agacccgctt
     7621 tttcgaatcg tttgatcatt tcttgttctg aaagaagata gacatttgct gttagtggta
     7681 aagatgcaga taaattttct aaaggtattt ggcttccctc tttgatatgt ttcatcactt
     7741 caagtgcaaa aagttcttct tgttgtaaac tagcgatggg atgtaatgca caaagcgaat
     7801 aattttcttt tatcacatac tgataccatt caggcaattg atcaggcgtg ttttctgtca
     7861 agccgatata aaaatttcta gtaaaaacat tttcaagtaa aaactgccaa tgaccgaaaa
     7921 agttatcctc tttattgaaa gcctcagaaa cagaatgatt tgccggaaca acaaacgcta
     7981 gatggtcggt ttctgtcatt tctgcaagtt ccaatttttc ttggatcatt tttcggctgg
     8041 aaagtttcat cagctgctga taacctattt catcttttgc gtacgcaagc aagccataat
     8101 ttttttcttc ataagtataa ctaagatgta atccaatgat tggctgtact ccttcttcta
     8161 cacaaatacg atagaattca ggtgcaccgt ataagacatc tttatccgtt atggctaatg
     8221 aagtgtagcc ttgttttttt gcttcttgta catattctcg aatacgtatc gtactttgta
     8281 aaagagaata agaagtaatt gtataaagtt gtgggaaaaa catcttgcat tccgcctttt
     8341 ttttcgttaa aattcactag cgttaaaagg tttgatcctt ttactttcat taagatagca
     8401 ggcgctgaag ttgattattc cacttcattt gcttgcttgt aatctgtagg aatgagttct
     8461 ttcgcaagaa ctcaaatttt attgagattt ggtctcgttt ctttacattc aaagaaaatc
     8521 tggtaaaatt aagatagaaa agaggtgttc tttatgcgat atatcgttac attattctgg
     8581 gctgtcgttc taggtcaagt agtcggttat attggtgctg cattaacaag cggaacatat
     8641 gattttactt tgacaacaat catttcattc attgcgggtg tcattatcct tttgattggt
     8701 gcagttgcgc caagaaaaga aacctctgcc cattcgtaaa aaaacagcca agagcttgag
     8761 atttctcaag ctctttttta ttttctactc ctcaccacca ttataatctg ctgcgaagaa
     8821 ctttacgaaa gttgaataaa gaaagaagaa tatttgacag aacaaacaaa cttgtcacaa
     8881 taaaaacagc actgtaatta taagcatgcg cgactgtcga tcctagcatg ggacctaaaa
     8941 cttgtccaaa gttgctaaac atctgattgt agctatagat tcggctgact ccttcttgag
     9001 gcgtaatctt actgatcagt gtattgacag aaggcatcaa agcgccagtt gaaaagccta
     9061 aaataaagcg aagtatacct aattggagcg gtgtagtgac gaatgccata ggtaaataac
     9121 agcaaaatga taaaatgagt cctccaatta gaactttttg attcccaatc ttatccccta
     9181 gttttcctag tttcggtgaa gaaaagacag cggaaacacc agctatcgaa acaatcaagc
     9241 cgctgacaaa aaggatattt tctgtacttc cgctcaattc acgaatatat aaggtcaaaa
     9301 tagggctgat cgtagtgatt cctatctgta aaatcaaggt cgtaatgaag agaccgatca
     9361 aaatagagag atgatccatt tttgaaaaaa tttctttggt gcttatcaga tcttttttct
     9421 cgattggatg gaaatcttct ttgactaaga aaatggtcaa taccgttgtg atcatcaaca
     9481 aagcccctgt gatcaagaat acattttcca taccaaacca ctgagccagt gcaccgccca
     9541 ttgaaggtcc gatcagattt ccagcaatcg ctccggtaga caatgttcct aacgcccatc
     9601 cgctcttttc tctaggtgcc tgtgaagcaa tcatcgctgt ggcatttgga atatatccag
     9661 acaaaatccc attccaaaac cgcatgatca aaagccaata cgcgtttggt acaaaagcca
     9721 aagatcccat tgttaatgtc atccctgctg ctgcacggat catcatgagt tttcggcctt
     9781 ttcgatcagc tagatttccc cagattggtg caaccacggc agatgcaaaa gcagtcacag
     9841 agattgctag tcccgaaaac aattcgattt gatcttttgg tgttcctagt tgctcaacat
     9901 aaactggtat gaatggcata actaaactaa tacttgcacc agtgaaaaaa cagccaatcc
     9961 acgcaatcac taaatttttt ctccaattaa tctccatcta tcatccctct ttcgtctctt
    10021 ttatcatacc tattttcgaa taaacaagca acgatctcgc cagtaaaaag agaaaaatcc
    10081 cgcaaaggtc attttatatg taacgttctt tttatgtaaa acaaaaagag atcagaacaa
    10141 atactgttcc aatcccttct ctttagttaa attgttgcta gaatgatgaa gttgataatg
    10201 aataaaatat caacgatcca caaagcaaca gaaacttgat tgtattttcc tttgattact
    10261 ttcacaattg tatagaaaat aaatcctgct gcaatcccat aggaaatgct atagcacaat
    10321 cccataaaga tcgatgcaaa gaaggccggt aaagcttctt ccatatctag ccatttgatg
    10381 tctgcaaaag aagccatcat cattactcca actaaaatca atgctggcgc agttgcttga
    10441 tttggtacaa tggcgatcag tggagagaat aaactactta atgcgaatag cactgccact
    10501 actactgaag tcaaaccagt acgtccaccg gccccgatcc cagctgcaga ttcgacataa
    10561 gttgttgtat tcgatgtacc aaagatagcc cccacagaag tagcgatcgc atcggcaaat
    10621 agtgctttat ccatttttgt tttgaaccca cgaccatctt ccaacgcgat ctcgtcttct
    10681 tttgagaaaa tacctgtgcg gcgtcctgta ccgataaatg ttccgatcgt atcgaaagta
    10741 tcagataaac tgaatgcgat gatcgtcatc aatacttgag gaatcttgga agaatcactg
    10801 aataaagatt gcatgccttc agaaccaaat gctgcaccga aagttgttcc taattcttta
    10861 aatgaattac ctaacgaatt cgtttgccaa tcaatggaac ttaaatcaac aacacccata
    10921 aaaataccaa ggactgtggt cgctacgata ccgataagaa tagccccacg gacatttttt
    10981 acaactaaaa tggctgtcag taccaaacca atcacagcca ataagatagg agcgttgtca
    11041 aagttggcta acgcagggac gattccacca tttatcgtaa catttgtcgc ttttccgcct
    11101 tcaacgacag agctcgaaat agctgactgg tctgctgaaa aagataagaa tcctgcattt
    11161 ttgattccga catacgcaac gaaaataccg atcccgccac caatcgcatg ttgaagactt
    11221 tcgggaatcg cccgaataat catttttcga atatttgtga cagtaataaa aatattgatt
    11281 aatccgcaaa taaacaccat tgccaaggct tgttgccaag aataaccaag cccaaaaacg
    11341 accgtaaaag tgaaaaatgc gttcaatccc ataccaggtg cctgagcata tggtacattt
    11401 gcgaacaatc ccattaccag cgtaccaata atcgatgcaa tgattgtcgc tagaaatact
    11461 gcttggaagg gcatacctga tgctgacaaa atcgtcggat ttacgaataa gatataactc
    11521 atggcaaaga atgtcgttac tcccgccatt atttcagttg atacatttgt attgtttttc
    11581 tttaaaccga aaaacttttc cataaaaagt tccactccct gaattttttt ctgatggaaa
    11641 actataataa taaaaaataa gtaaaatagg gcaaatccat cgaacaattt caaattatat
    11701 agacgtttca ttcgcttttc aacgtttttt taacaaaaaa agcgaaaata atacttaacg
    11761 ttcgtgttta gcttatgttt agctatttat atagccataa ctgtagtttt tgttaggaat
    11821 agctgattgc tcttgttaat tacctaaaat atgctagact aaaacaaatg ctagttgagg
    11881 aaggaaggac ccttattgaa taaaaaatta attgccatcg atctggacgg aactaccctc
    11941 aatcaagatt cgatgattac atctaaaaca aaagagactc taaaaaaagc aatcaatgcc
    12001 ggtcatcatg tcagtattgc tacaggacgc ccattccgaa tgagccatca gttttaccaa
    12061 caattagaat taacaacacc gatggttaat tttaacggcg cattagtaca catccctaat
    12121 cagcactggg atggtgaaag agaaacgttg atcaatcgtg aaatcgcttt tgaaatctta
    12181 tcgcaaaaaa agcagctaaa tttagatttt atcgctgctg aaaatcgtga tacttttttt
    12241 attgacagtt ttgatttctt tgatgaaaaa atttttgctt cttctcgtcc aggagagaag
    12301 aatttgcttt ctcctaaaaa tttgacgaca aatcctacat ctttgcttgt ccgaacagac
    12361 aaacgttttg caggagctgt atctgctgaa ttaacacgtc aatttggcag ttatgtcgat
    12421 gttcggacat ggggtggacc tactgccatc ttagagattg tttcaaaagg aatccaaaaa
    12481 gcaaaaggcg tgcaagagat tgctaactac ctgtctatcg atcaaaaaga cgtcattgcc
    12541 tttggtgacg agcataatga tttggaattg ttggattatg caggttgggg agtagctatg
    12601 gctaacggta ctgaccaact caaaggcatt gcaaatgatg tcactccttt gagcaatcag
    12661 gaagacggtt tagctgttta tctggaaaaa ctacttaaac tataatagag aattagattc
    12721 cacaaaaaag aggttgtgac aattttt
//
LOCUS       DAZKLA010000077        12738 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000077 DAZKLA010000000
VERSION     DAZKLA010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.078"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..774
                     /locus_tag="ACG85N_001860"
     CDS             37..774
                     /locus_tag="ACG85N_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224624.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            788..1603
                     /locus_tag="ACG85N_001861"
     CDS             788..1603
                     /locus_tag="ACG85N_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0224625.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1616..2263
                     /locus_tag="ACG85N_001862"
     CDS             1616..2263
                     /locus_tag="ACG85N_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0224626.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2279..2935
                     /locus_tag="ACG85N_001863"
     CDS             2279..2935
                     /locus_tag="ACG85N_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0224627.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3097..3918
                     /gene="racE"
                     /locus_tag="ACG85N_001864"
     CDS             3097..3918
                     /gene="racE"
                     /locus_tag="ACG85N_001864"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFQ0224628.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3921..5276
                     /gene="rph"
                     /locus_tag="ACG85N_001865"
     CDS             3921..5276
                     /gene="rph"
                     /locus_tag="ACG85N_001865"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFQ0224629.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5277..5795
                     /locus_tag="ACG85N_001866"
     CDS             5277..5795
                     /locus_tag="ACG85N_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0224630.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5902..6402
                     /gene="cbpB"
                     /locus_tag="ACG85N_001867"
     CDS             5902..6402
                     /gene="cbpB"
                     /locus_tag="ACG85N_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFQ0224631.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(6682..7602)
                     /locus_tag="ACG85N_001868"
     CDS             complement(6682..7602)
                     /locus_tag="ACG85N_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFQ0224632.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            7728..8780
                     /locus_tag="ACG85N_001869"
     CDS             7728..8780
                     /locus_tag="ACG85N_001869"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFQ0224633.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(8890..9645)
                     /gene="fabI"
                     /locus_tag="ACG85N_001870"
     CDS             complement(8890..9645)
                     /gene="fabI"
                     /locus_tag="ACG85N_001870"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFQ0224634.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            9865..10305
                     /gene="fabZ"
                     /locus_tag="ACG85N_001871"
     CDS             9865..10305
                     /gene="fabZ"
                     /locus_tag="ACG85N_001871"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0224635.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(10420..11181)
                     /locus_tag="ACG85N_001872"
     CDS             complement(10420..11181)
                     /locus_tag="ACG85N_001872"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0224636.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            11370..12464
                     /locus_tag="ACG85N_001873"
     CDS             11370..12464
                     /locus_tag="ACG85N_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFQ0224637.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(12550..>12738)
                     /locus_tag="ACG85N_001874"
     CDS             complement(12550..>12738)
                     /locus_tag="ACG85N_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0224638.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
BASE COUNT         4102 a         2206 c         2676 g         3754 t
ORIGIN      
        1 agaaatctta ctaaataata atgggagtgt aattctatgg caatgattaa atttgatcac
       61 gtggataaat attatggcaa gttccacgca ttaaaaaata tcaatctcga attcgagaaa
      121 ggagaagttg tcgttgtcat tggtccttct ggttcaggaa agagcacgat gcttcgctgt
      181 atcaatggat tagagacgat ctcttcaggt aagctgctga ttaatgatac tgacttgcat
      241 gataaaaaga caaaactgac agaagtccga aaaaacatcg gcatggtttt ccaacatttc
      301 aatttgtacc caaataaaac tgttctggaa aacatcaaat tagcaccaat caaagtgttg
      361 aagcaagacc aggcaactgc agtcaaaaac gcggagaaat ttttgaaaac tgtcaatatg
      421 ttagataaga aagattctta tccttccatg ttatccgggg gacaacagca acgggtggca
      481 atcgccagag gtttagcgat gaatcctgag atgctgctgt ttgatgaacc tacttctgct
      541 ttggatccgg aaatgattgg tgatgtatta gacgttatga aaaaattggc tcatgacggt
      601 atgtcaatga ttgtcgtgac acatgaaatg ggctttgcca aagaagtggc ggatcgtgtt
      661 atcttcatgg ctgatggaga agtattggaa gacagcagaa atgtgagaga ctttttcgaa
      721 aatccaaatg aagtccgagc acagcaattt atcagtaaag ttatcaatca ttgataggag
      781 gaaaaagatg cgcaggttaa aaaaaatacg cctgattttc cttttaggca ttgcttttct
      841 tgcactagcc ggatgtaaag gaaatgggtt ggcaaatgaa gatattttag agcgaagcaa
      901 atctaccaat gaaatcatct ggggtgtcaa gtatgatact cgattatttg gaatgatgga
      961 tattgagagc agaactgtac aaggatttga tgtagatatt gcaaaagcaa tcactaaaaa
     1021 gatccttggt gacaatggaa aaacagaatt tgttgaagtc acttctaaaa ctcggattcc
     1081 attattgaaa aacggaaata tcgatgcaat tattgcaacg atgacgatca cagatgaacg
     1141 aaaaaaacag gttgactttt cagatgtcta tttcgatgct ggccaagctt tgttggtaaa
     1201 gaaaggaagc caaatcaaaa gtgtagatga tttgaatgct tctacaacgg ttctagcagt
     1261 caaaggttct acatcggctg cgaatatccg acagcatgca ccagatgcaa aaattttaga
     1321 attagaaaac tatgcagaag catttactgc tttacaatct ggtcagggag acgcgatgac
     1381 tacggataat gcgattttgt tagggattgc agatgaaaat ccagagtatg agttagttgg
     1441 tggaacgttt actaatgagc cttacgggat tgcgatcaac aaagggcaag aaaacttctt
     1501 gaaagctgtt aaccaagcgc tagaggaaat gcatgcagat ggcacatacg ataaaatcta
     1561 tcaaaaatgg tttccaaatg aaactgaagg aaaagtagag taagggggag aggcaatgag
     1621 cgaattgata caaacttatg gaccaatctt tgtcgacggg ttcaagatca cattgttttc
     1681 gagtgtctta gcacttgtgt ttagtttgat tatcggaacg ttaatggcaa tcttccaatt
     1741 atcaaagaat aaagtagtga gcgccttagc aaaggcatat gtagaatttt ttagaaacat
     1801 tccattatta atcatcgtga tgttttttta tgttgtcgta ccgatttatt ggtttaatct
     1861 agatggattc caagcaggta cgattggatt aacgatctat acatctgctt ttattgctga
     1921 aacagtacgt tcagggattc aaaccgtacc aaaaggtcaa acagaagcag gactatcttc
     1981 tggttttacc tattcagaaa tcatgcgtta tattgtttta ccgcaagcat tcaaaatcgt
     2041 tatcccgcca ttaggcaacc aatttatcaa cctcgtaaaa aactcatcta ttttagcaat
     2101 cgtagctggt ttagatttga tgtatcaagg agacttgatc gctagtgaaa cattcaatac
     2161 cttcgatacg tatatcatcg ttgggttatt ttacttagtt atcacgctac cgttatctta
     2221 cttaatggtt tatcttgaga aaaaatgggc ggttcgttca tagaaggagg ggaaatacat
     2281 ggattttagc ggcgcttttt catggatgaa tatccgtttt ttattggaag gattaaaggt
     2341 tactgttgaa gtttcattgt tttcgatcat ttttagtttc ttgatcggtg gtctgacagg
     2401 tgtgattcgt tttgcgaata tccctttctt ttccaaaatc cttggtgtag tgattgatat
     2461 tatacggaat cttccattat tattgatcat tttcttcact tactttgcct tacctcaaat
     2521 cggcattcag atgaacatct tctggtccgc tgtcgcagca ttaacgattt ttgagtcagc
     2581 tatgttgtcg gaaattttcc gagctggact caatgctgtt ccaaaaggac agatggaagc
     2641 aggattatca acgggactga cttatgtaga atcgatgcga acgatcgtgt taccgcaagc
     2701 atttaaatca atgatcccgg caattgtgag ccaattgatt tcactgatca aagatacttc
     2761 attagcagta atcatttctt tacctgaact ttctcatcag gcacgtatta tttatggaca
     2821 gaacaccaat tatgtgcttc cgatgttcat catcatgaca cttatgtact tcgtggtatg
     2881 ttacgcgctt tctctgcttt ccggttactt ggaaaagaga cggtacagtt attaatagat
     2941 gattctttta gcggttgtgg caaactaaag tcacaaccgc ttttcctctt tccattcgaa
     3001 aaaaagagat ttcgattgtt ttaagcaagt aagagcactt gaaaattcac gctaaaaagt
     3061 acacaatagg gaggatagaa tttattgaaa aaaaggatga tacgattgac agataatcgc
     3121 cctatcggat ttattgattc aggtgtcggc ggcttgactg tagtaaaaga agccctgaaa
     3181 caattaccga atgaaaatat tttatttgta ggagacacag cacgctgccc atatggccct
     3241 agacccgcgg aacaggtaat ccagtatact tgggaaatga cggattatct ggtggagcaa
     3301 ggaatcaaga tgctggtgat cgcctgcaat accgcaactg cggtggcttt agaagaaatc
     3361 aaagctgctc tttctattcc agtcatcggt gtgatccttc ccggtactag agcggcagta
     3421 aaaaaaacac aaaataaaca agttggcatt atcggtacga ttggtacggt aaaaagtcaa
     3481 gcttatgaaa aagcactgaa agagaaagta ccagaattga ctgtgacaag tcttgcttgt
     3541 cccaaatttg tttcagttgt cgaaagtaat gaataccatt catcggtggc gaaaaaaatt
     3601 gtggcagaaa cattagctcc tttaaccact aaaaaaatcg atacattgat tttgggatgc
     3661 acccattatc cattattacg ccccatcatt caaaatgtaa tgggagaaaa tgttcaactg
     3721 atcgattctg gagcagaaac agtaggtgaa gtatctatgc tgttagatta tttcaatttg
     3781 agcaattcac cgcaaaatgg tcggacatta tgccagtttt atacaactgg ctctgccaaa
     3841 cttttcgagg aaatagctga agactggctt ggaatcggac acttaaatgt agaacatatc
     3901 gaattgggag gaaaataagg atgcgtcatg acgggagaaa agcccaagag cttcgcaaac
     3961 tggagcttca aacaaatgtt ttcaaatatc cagaaggatc ggtagtgatc cgttttggtg
     4021 atacaacagt tatttgttct gcaacaatag aaaattctgt tccccctttt ttgagagaaa
     4081 caggaacagg atgggtgaca gcagaatata gtatgctgcc tcgagcaacg agtacgagaa
     4141 atcgtagaga aagcagcaaa gggaaacttt ctggtagaac aatggaaatc cagcgtctta
     4201 ttggtcgttc cttgcgtgca gtagtagact tggaaaaatt aggagaacgt agtatcatcg
     4261 tagactgcga tgtgatccaa gcggatggcg gtactagaac agcaagtatc actggggcat
     4321 ttgtggcatt gaaattagcc attgaaaaac ttttaagaga aaaagaattg tcagaagatc
     4381 caataaaaga acatcttgct gcagttagcg taggaatctt accagatggg acttgcgtca
     4441 cagatttgga ttaccaagaa gattccgcag cacttgtaga catgaatctt gtaatgacag
     4501 aatctggaaa attcgtggag attcaaggaa ctggagaaga agcaacgttt gatggtgaac
     4561 agttgaatga aatgctcttt tttggaaaaa acgcgattga agatttgatc aaagagcaaa
     4621 aacatgcttt gctaacagag tttgctcaaa atgatgagcg gatagaagaa acaaaaacaa
     4681 tcatcattgc gacaagaaat ccaggaaaag cagaagagtt tcgaaatatg ttcaaagaag
     4741 cgggatatca tgtaaaaaca ttacttgact atcctgaatt gccagatgtg gaagaaactg
     4801 gcagcacatt tgaagaaaat gcccgattaa aagcagaaac gattgcacaa ttgcttgatc
     4861 agccagtctt agcagatgat tcaggtttga aagtggatgc tttaggcggt atgccaggaa
     4921 tctattctgc acgttttgca ggggaacaaa aaagtgatgc ggggaataac gccaaattat
     4981 tatatgaatt gacagatgtg ccggatgaaa aaagaacagc acaattccat tgtacgttag
     5041 tattcgcggc acccaaaaaa gacagccttg tagtagaagc ggaatggccc ggtcgagtag
     5101 cccgtatacc atctggcgaa aatggctttg ggtatgatcc tttatttatc ccagaaggta
     5161 agaaacaaac agcagcagaa ttatcgagcg aagaaaaaaa caagatcagt catcgggctc
     5221 aagcaatgaa aaaattgagt gccgagtgga aacaatggct ggaaggagag agatagatgc
     5281 ggtatttagt agtaagtgat aatcatggag acagagatat cctcgttgac ttagtagatc
     5341 gttatcgttc tgaagtggat atctttttcc attgtggtga ttcggaatta gaagcttctg
     5401 acccattatg ggatacattc caagtggttc aaggtaattg tgattatgga ccaggattcg
     5461 aacagaaaaa agtgatccaa actggtcagg atacagtatt catgactcac ggtcatttat
     5521 cgaatgtccg atttggtttg acacagttag caatcgaagc tcgagctgca actgccgata
     5581 tggtgttttt tggtcatacc catcaaatag gctgtgagat ggatcagaaa atcctgtttt
     5641 tgaatcctgg tagcatttct cagcctagag gcccgatcca aatcccatca tatgctctta
     5701 tcgaaagttt agatgaccaa atttctgttc aatactataa ccgtgcccat caggcagttg
     5761 aagagttggc cttttctttt tcgagagagc gctgaataaa aaaactctaa tttttaataa
     5821 aaataagctt tattttatga attttgcatc cggtttcggt aaaatagtag tgttgaatga
     5881 aatagaaatg gaggccatca gatgattgga cctatagtaa gagaaatgct gctggaaaat
     5941 caggaaacgt ttttgattcc tgcagaaaat gtggcaaatg ttatgtatca tcatccattg
     6001 tcccatggtt tacttgtgtt atcgaaggtc ggttattcca aaattcctgt attaggaaaa
     6061 gatgatcact ttgtaggact agtaagttta tcagatgtgg tcaataaaat gattgacttg
     6121 caaacaatca gcatggaccc gttagaagga ctaacggttg ctgatgtgac agaaacagat
     6181 gttccgacaa ttgatgaaaa ttgggaacta gaagaagtac tccatttgtt agtcgattcg
     6241 ccttttttac cggttgtcac agaagatcgg attttcaaag gaatcattac aagaaaagag
     6301 ctgcttaaag ccgtaaactt catggtgcat gaattagaac gtcgtaatat attgtctccg
     6361 aaaatcgaag aattaaaaga acaaatcgat atcgtgagct aaagaacgag aaactaaaag
     6421 ataatcaaaa aataagtaga aaaaacagcg taccagtttt gactttatct tgaaaaagag
     6481 gctgggacaa aagcgtttaa ctccaagaaa taagaaggaa ttcacgaaaa ttgcttttca
     6541 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc cgtttatacg
     6601 tgtttagagc gtgggacaaa agtgttcttt acttttgtcc cacgctcttt ttagtgagat
     6661 tcgttgcatc gtttgtccaa attattttcc gacagcaatt ggactgttcg gaatcgtgaa
     6721 accagaagct gtcaattctt tgacatagga gctcaagaac tcttctttta aggcatattg
     6781 ttttccgttc aatgcgtagc agaccgtacg aatcgcaaaa ttgctgtttc caagatccac
     6841 taatccaaag atagaaggtt cagtttggag ctcttcttgg tatttttcag ctaatgtacg
     6901 atttactcga tcaatgatct catagatttt gtcatatcct tcttcaggaa caatccgaat
     6961 atctaaaagt acttgcatat ttgcacgaga gagattgctg atcgtggtga tgtttcgatt
     7021 cgggatgaaa tggacagtac catccgttgc tttcagctga agggtacgga taccaacaga
     7081 tacgacggtt ccttcaatcg ttaagtttgc tagcttgatg tagtccccaa catccatctg
     7141 ttgttccatg ataataaaaa atccggtaat aacatcactc atgaaccctt gggcaccaag
     7201 accgattgcc acacctgcaa ttccggcacc agccaaaagt gatccgatcg gcacaccgat
     7261 agcagataaa attgcataga taaaaaagaa accaagggta taatgaaaaa tagtatttaa
     7321 aagactatgt aaagtcttta agcggctctc gtttagaatg gcttttttag aatactgatt
     7381 aaaagactta tcaatcagat attttcccac acggtgaaga atccaaaaaa ggacgatcaa
     7441 aaaaatcaga tataaacttt tttggataaa ggcagccaaa atggcgtccc aatcaatccc
     7501 gtcccaaaac cgctgaaacg catttacttt gctgacagct tgatctgtca atgtctcgcc
     7561 agaagagtct gtcgtaccgg cagttgctaa taatgaaaac ataaaatcct cttttctaaa
     7621 atttgattct ttctgtattg taccaagaag ttttcaaaaa aggaaatagc aaaaataact
     7681 ataatgaagg aaaaaagctg aaaataaaat gacaaataat atggataatg ataaaatgtg
     7741 gagaaaataa cgaaggtggt aagtatatgc aaatcgattg gaacaatcta ggtttttctt
     7801 atatcaaaac tccttggcgt tttattgcca aatggaaaga cggacaatgg gaagaaggag
     7861 aattgacaga agataattac ctgacgattc atgaaggctc tccagcgctt cattatggtc
     7921 agcaatgttt tgaaggtcta aaagcttatc gtcgaaaaga cggcaaggtc aatctctttc
     7981 gacctgaaca aaatagtcga cggatgaatc aaagtgctag acgattgctg atgccaatgg
     8041 tccctgaaaa gatgttcgtt gatgctgtca agcaagtggt aaaagcaaat gaagattttg
     8101 tgccgcccta tggaacaggc gctacattat acttgcgccc tttactgatt ggggtcggtg
     8161 aaaacattgg tgtccatcca gctccagaat atttatttgt cgtcttctgt acgccagtag
     8221 gagcgtattt caaaggtgga ctaaaaccaa cgaattttat tgtatctgat tacgatcgtg
     8281 ctgctcctaa ggggactggt gcagcaaaag tcggcggaaa ttacgcagcg agtttacttc
     8341 caggaacaga agcaaaagaa ctggattact cagattgtgt ttatcttgat ccagccacac
     8401 atacgaaaat cgaagaagta ggtgcagcaa atttctttgg aattacaaaa gacggaaaat
     8461 tcatcactcc gcaatcgccc tctatcttac caagtatcac gaaatattcc ttgttgcagt
     8521 tagctgaaga acgactagga ctgcaagcaa ttgaaggaga tattttcatt gatgatctag
     8581 atcaatttgc agaagctggt gcatgtggta cagcggctgt tatttctcca atcggtggga
     8641 tttatcacaa taacaatctt catgtatttt atagtgaaac ggaagtagga ccagtaacaa
     8701 aacggttgta tgatgaattg acaggtattc aatttggaga catagaagca ccagaaggct
     8761 ggattgaagt cgtggaataa tttcaaagta atgagttgaa aaaaacgtaa aaagaaaaaa
     8821 tcaggaagag actttaaaaa gttttttcct gatttttata tgttttactt taaagaatgc
     8881 aacaaaagtt tatgaaagat ggacaccttt gtcaacgtag ataatatcac cgatgattcc
     8941 gctggaaagt gggctgatta agaaagcaca agcattaccg acttcttcga tcgtcacacc
     9001 ttgctgatca ggcgtacgag attcagataa ctggatcagt ttttgatagt ctttaacacc
     9061 tgtgacagct aatgttttga ttgccccagc tgagattcca ttgacccgaa ttcctttagg
     9121 cgagaactca gctgccaaat aacggatagc agcttcaaga gaagctttcg caatacccat
     9181 catgttataa ttcgggattg cgcgttctga acctagataa gtcatgctaa cgatcccgct
     9241 gtttggtgca agaaacggtt gtgcgtattt tgatacagca attaaagagt agctgctgat
     9301 atcttgagct aattgatagc cgtctctgga aatatcagac acatttccgg acaattcttc
     9361 tttgttggcg taagcaactg catggacaag gccgtggatc tccccagcat attcaccgat
     9421 ggcattcatt gctttttcga tgctttcgtc gctagaaaca tcacattcta cagtgaatgc
     9481 ttcttcgccg accaatttga ctaatgattt tttcattcgt tcattttggt aagtatagat
     9541 aatttctgcc ccttgtttca acattgcttc agcacaaccc caagcaatgc tttttttgtt
     9601 agcgacgccc ataatgacga ttttttttcc ttctagaaat gacataatga ctctcctttg
     9661 tttctgataa aaatttgata gtcgtaaaaa aatagagaat aatagctaaa ataacttctt
     9721 gatttcttaa ttattagtca aaatgttttg attgtcaaac tattttttct taaattaaaa
     9781 aaagattgct aaatatttag gaatatgata ctttgatagt caaagtaatg aaaatggcaa
     9841 ttgcctgaaa gaaggagttt aattatgtct tacgtattat ctgctactga agtaatggaa
     9901 ttgattccta atcgttatcc tatttgttat attgattatg tagatacttt agaaccagga
     9961 aaaaagatcg ttgcgacaaa aaatgtaaca atcaatgaag attttttcca aggtcacttt
    10021 cctgacaatc cagtgatgcc gggtgcgttg attctagaaa cactggcaca agcaggttcg
    10081 atacttatcc taaaatcaga agagtttcta ggaaaaagag cttatatcgg tgggatcaat
    10141 aaggccaaat tccgtcaaaa agttgttcca ggagatgtgc tgaaatgtca ttttgaaatc
    10201 accaaaatga agggatcagt cggtacagcg ttatctgcag catatgtaga tgataaaaaa
    10261 gtctgtgagt gcgagttcac gttcatcgtc aaccaaaatg cttgactata aaacctgata
    10321 tagcaaaaca aaaggaagct tacttgcttt ttatgtaaaa agaagacgcc tgaatcctag
    10381 catacgttgt catctatgca ggattcagac gttatttttt tatttttgtt ttgcttcttg
    10441 cagtatattg attttatcga tttttacttc ttcttttggt ttgtctgaac tatctgtttc
    10501 gatatttgca atagcatcca cgacatccat tccttcaatc acttggccga agacagtata
    10561 atggccatct agtgaaggat aaccgccgtt tttataagcg tcgatgattg cttgaggata
    10621 gtcgtctttt aaaagaccat catgcatatc atctgtattt tgaacgataa agaactggct
    10681 gccgttactg ttaggatctt gtgagcgagc catagataac gcacctctta tattataaag
    10741 gtggttgttg atctctgttt caaatccttc tccccaaatg ctttcccctc cagtgccatc
    10801 gcctttagga tcgccacctt ggatcatgaa atctttgatg acgcgatgga aagtcagtcc
    10861 gttgtagtaa ccgtcttttg catgtgtcat aaagttttca accgcttttg gtgcgtgttc
    10921 gggaaataat ttgattttga ttgtgccttt attcgtcacc atttcgacca gatcttcatt
    10981 ttcggcgaca tctttcgtca attgtggtaa ttccaatgcg ttcaaatcga cgctactggc
    11041 agtcgtgctt tccgcagtag atgtactttt tgaactttct gtagacgaac tagaagaagt
    11101 cgtgttgttt gcagaacaac cagcagcaaa cagcaatgct gctgctaaac taatacttaa
    11161 aataacttta tttttcttca taactgttcc tttccaattt actattttgt tatctttaat
    11221 gataacaaaa tagagggaaa atacctactt aaattattgg aatttatcat ttattttaaa
    11281 agaaaaagaa aaggtgtaga ccatttactt tgtgtttgct ataatgtaag tggatacaaa
    11341 aacgaaaaga tgcaggagga aatggtaata tgggaaaatt aggaatctcg atttatccag
    11401 aacggtcaac atttgaaaag gacaaagcgt acttggattt agctcataag tatgggtata
    11461 aacgggtttt taccagtttg ctgcagatca atgatgataa agaaaaagtt ctttcagaat
    11521 ttaaagaagt agttgattac gcgaatagcc taggcatgga agtaatggtt gatatcaatc
    11581 cagcgttatt tgaacagttg gagatttctt acgatgactt atcttttttc cataagatgg
    11641 gtgcctacgg ggtccgctta gatatcggat tcacaggagc agaagaagca aaaatgacgc
    11701 gcaatccttt tggtatcaaa atcgagatca atatgagttc gggcacgaat tacgttgaca
    11761 acatcatgag ttattcgcca aacacggata atttattagg ttctcataat ttttatcctc
    11821 atcgatactc tggtttagga tacgaacact ttgttttttg ttcagaaaaa ttcagaaaat
    11881 ataatttgaa tacgatggca ttcgtcaatt cgcaatctgc tgagtttggc ccttggccta
    11941 cgcaagatgg cttgtgcacg ctggaagacc accgagatct tgaaattgcg acacaagtga
    12001 agcatttaat ccttacagga ttgattgatg atatttctat tggaaatgcg tacgcaagtg
    12061 aagaagaatt gaaagaaatg gcagaagcat ttaacgcgga ttatccaaca ttgaaagtag
    12121 atacggaaga aggtattaca gaaaacgaac gaatctgctt gtttgataac cttcatagtt
    12181 atcgagggga tcgctcagaa tatatccttc gttcaacgat gactcgtgta tactacaaag
    12241 ataaagattt cccgcctcat aatacgcgag atatgcatca tggggatgta ctgattgaca
    12301 atgaagggta cggtcaatac aaaggagaaa cacaaatcgc cttgaaggat atgaaaaacg
    12361 acggacgtgt caatgttgta ggaagaatct ccgatgacga attattcctc cttgactttt
    12421 tgaaaccatg gtcaagcttt aaattaattg aaaataaaaa atagataatt agttattgca
    12481 gaaataatcc gctagaaata gccagttatt tctgcttttt tattttgttt ttttataggt
    12541 tatgatttgt caaattaagc tagaataaaa tgcttcttgt acatagctca aaaatatttc
    12601 gattggtgtt ctgtaattca atgattttct tggaatatga ttacgttgat tgctgacact
    12661 ggaaataaat gtctgattca cttttctaaa atccattgat ttcggcagtc cattacgacg
    12721 cagaatcccg ttagaatt
//
LOCUS       DAZKLA010000078        12556 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000078 DAZKLA010000000
VERSION     DAZKLA010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.079"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..776
                     /locus_tag="ACG85N_001875"
     CDS             <1..776
                     /locus_tag="ACG85N_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0224639.1"
                     /translation="DSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYL
                     VKITEEKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANAD
                     VEKELYANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMT
                     RRLEPMDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFF
                     KVSLDEIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            848..1969
                     /locus_tag="ACG85N_001876"
     CDS             848..1969
                     /locus_tag="ACG85N_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0224640.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVS
                     LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELI
                     SLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIK
                     NNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(2051..3823)
                     /locus_tag="ACG85N_001877"
     CDS             complement(2051..3823)
                     /locus_tag="ACG85N_001877"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224641.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(3826..5538)
                     /locus_tag="ACG85N_001878"
     CDS             complement(3826..5538)
                     /locus_tag="ACG85N_001878"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224642.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            5653..6321
                     /locus_tag="ACG85N_001879"
     CDS             5653..6321
                     /locus_tag="ACG85N_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFQ0224643.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            complement(6388..7176)
                     /locus_tag="ACG85N_001880"
     CDS             complement(6388..7176)
                     /locus_tag="ACG85N_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0224644.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(7259..8731)
                     /locus_tag="ACG85N_001881"
     CDS             complement(7259..8731)
                     /locus_tag="ACG85N_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0224645.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            complement(8861..10054)
                     /gene="metK"
                     /locus_tag="ACG85N_001882"
     CDS             complement(8861..10054)
                     /gene="metK"
                     /locus_tag="ACG85N_001882"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFQ0224646.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     regulatory      complement(10052..10131)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            complement(10320..10391)
                     /locus_tag="ACG85N_001883"
     tRNA            complement(10320..10391)
                     /locus_tag="ACG85N_001883"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10356..10358),aa:Glu,seq:ttc)
     gene            complement(10403..10476)
                     /locus_tag="ACG85N_001884"
     tRNA            complement(10403..10476)
                     /locus_tag="ACG85N_001884"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10440..10442),aa:Ile,seq:gat)
     gene            complement(10489..10559)
                     /locus_tag="ACG85N_001885"
     tRNA            complement(10489..10559)
                     /locus_tag="ACG85N_001885"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10525..10527),aa:Gly,seq:tcc)
     gene            complement(10564..10636)
                     /locus_tag="ACG85N_001886"
     tRNA            complement(10564..10636)
                     /locus_tag="ACG85N_001886"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10601..10603),aa:Phe,seq:gaa)
     gene            complement(10640..10712)
                     /locus_tag="ACG85N_001887"
     tRNA            complement(10640..10712)
                     /locus_tag="ACG85N_001887"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10677..10679),aa:Asp,seq:gtc)
     gene            complement(10731..10804)
                     /locus_tag="ACG85N_001888"
     tRNA            complement(10731..10804)
                     /locus_tag="ACG85N_001888"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10768..10770),aa:Met,seq:cat)
     gene            complement(10817..10906)
                     /locus_tag="ACG85N_001889"
     tRNA            complement(10817..10906)
                     /locus_tag="ACG85N_001889"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10868..10870),aa:Ser,seq:tga)
     gene            complement(10929..11000)
                     /locus_tag="ACG85N_001890"
     tRNA            complement(10929..11000)
                     /locus_tag="ACG85N_001890"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10965..10967),aa:Glu,seq:ttc)
     gene            complement(11022..11109)
                     /locus_tag="ACG85N_001891"
     tRNA            complement(11022..11109)
                     /locus_tag="ACG85N_001891"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11073..11075),aa:Ser,seq:gct)
     gene            complement(11134..11207)
                     /locus_tag="ACG85N_001892"
     tRNA            complement(11134..11207)
                     /locus_tag="ACG85N_001892"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11171..11173),aa:Ile,seq:gat)
     gene            complement(11221..11291)
                     /locus_tag="ACG85N_001893"
     tRNA            complement(11221..11291)
                     /locus_tag="ACG85N_001893"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11257..11259),aa:Gly,seq:tcc)
     gene            complement(11293..11368)
                     /locus_tag="ACG85N_001894"
     tRNA            complement(11293..11368)
                     /locus_tag="ACG85N_001894"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11333..11335),aa:Asp,seq:gtc)
     gene            complement(11387..11460)
                     /locus_tag="ACG85N_001895"
     tRNA            complement(11387..11460)
                     /locus_tag="ACG85N_001895"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11424..11426),aa:Met,seq:cat)
     gene            complement(11473..11562)
                     /locus_tag="ACG85N_001896"
     tRNA            complement(11473..11562)
                     /locus_tag="ACG85N_001896"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11524..11526),aa:Ser,seq:tga)
     gene            complement(11586..11659)
                     /locus_tag="ACG85N_001897"
     tRNA            complement(11586..11659)
                     /locus_tag="ACG85N_001897"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11623..11625),aa:Ile,seq:cat)
     gene            complement(11673..11746)
                     /locus_tag="ACG85N_001898"
     tRNA            complement(11673..11746)
                     /locus_tag="ACG85N_001898"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11710..11712),aa:Met,seq:cat)
     gene            complement(11774..11846)
                     /locus_tag="ACG85N_001899"
     tRNA            complement(11774..11846)
                     /locus_tag="ACG85N_001899"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11811..11813),aa:Ala,seq:tgc)
     gene            complement(11866..11939)
                     /locus_tag="ACG85N_001900"
     tRNA            complement(11866..11939)
                     /locus_tag="ACG85N_001900"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11903..11905),aa:Pro,seq:tgg)
     gene            complement(11949..12022)
                     /locus_tag="ACG85N_001901"
     tRNA            complement(11949..12022)
                     /locus_tag="ACG85N_001901"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11986..11988),aa:Arg,seq:acg)
     gene            complement(12033..12118)
                     /locus_tag="ACG85N_001902"
     tRNA            complement(12033..12118)
                     /locus_tag="ACG85N_001902"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12082..12084),aa:Leu,seq:taa)
     gene            complement(12135..12206)
                     /locus_tag="ACG85N_001903"
     tRNA            complement(12135..12206)
                     /locus_tag="ACG85N_001903"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12172..12174),aa:Gly,seq:gcc)
     gene            complement(12218..12290)
                     /locus_tag="ACG85N_001904"
     tRNA            complement(12218..12290)
                     /locus_tag="ACG85N_001904"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12255..12257),aa:Thr,seq:tgt)
     gene            complement(12308..12389)
                     /locus_tag="ACG85N_001905"
     tRNA            complement(12308..12389)
                     /locus_tag="ACG85N_001905"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12353..12355),aa:Leu,seq:tag)
     gene            complement(12397..12469)
                     /locus_tag="ACG85N_001906"
     tRNA            complement(12397..12469)
                     /locus_tag="ACG85N_001906"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12434..12436),aa:Lys,seq:ttt)
     gene            complement(12473..12545)
                     /locus_tag="ACG85N_001907"
     tRNA            complement(12473..12545)
                     /locus_tag="ACG85N_001907"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12510..12512),aa:Val,seq:tac)
BASE COUNT         4022 a         2698 c         2225 g         3611 t
ORIGIN      
        1 tagactcgac tagggaaaaa atcataaaaa tatttgataa aattaataat ttattcaaga
       61 atgacaatgc tgaattaagg aagggtcttt tagagtttaa acttcaagaa ctagaacttc
      121 attactctta tttagtgaaa attaccgaag aaaaagaaca acaaaaagca ataaaagaac
      181 agatgataga agaggaaaaa gttcgtagag aaattgatcg tgaaaagaaa cgtattgata
      241 aagaacaacg tcaattcaat tctgaaattt ccaaattaat cgcatatatg caaaaagcta
      301 atgcagatgt ggaaaaagaa ttatatgcaa ataaaattca agaactggaa gaaaaattaa
      361 aagaattaga agttatacgt gaaaatgtat tacaaagaga attaaatact agagccggtt
      421 atgtgtatgt aattagtaat attgggtctt ttggtgaaga catatataaa attggtatga
      481 ctagaagatt agaacctatg gatagaataa aagaattgag tagtgcatcg gttccatttg
      541 aatttgacgt tcatgcgatg attttttcag aggacgctcc gtctttggaa actaaattgc
      601 ataatcattt tagaaaacaa gaagttaata aaattaatca aagaaaagaa tttttcaaag
      661 tttcactaga cgaaatagag aaagttgttt tagaaaatta taatggaaca gtaacattta
      721 caaaattagc taaagctgaa caatatagac gatctttaga attgtcaaaa gattaaatat
      781 ataaaaagcc ttcgggcttt ttcttttaac aattaatagg aacgtatatt cgaaaggaat
      841 agaaataatg tggttagaaa aaataaatga caaaaaattt aagtacaccg aacgttatac
      901 ggatccctta actgaaaaaa agagaaaagt gtccgttact ttatctagca attcacgcca
      961 agcttggaat caagctaact tacttttgaa tgaaaaaatc gcagaaaaaa taaaacgaaa
     1021 tgaagaatta ccactaactt ttggagaatt aaaaactaaa tgggatgaaa aatacaagcc
     1081 aactgtaaaa gaaagttcct atcgaacaac tcaggtttat ctatcattaa tttcaaaata
     1141 tataaaagat gatgtacttg taaaaaacgt taatagtaat cttatacaag atatgctaga
     1201 ttactactat tttgaaaaaa atttatcata taattatgta atacatttaa aaacttttac
     1261 aggaatgatt tttaaattcg ccaaccgtcg atatggatta aaatataatc ccgtaaatgg
     1321 ggtttcttta gcaagaaaac ctaagacaaa cgaagaaata aaaaaagaaa aagatggtta
     1381 tttatcaaaa gaagaagttc aaatgattgc tagtaaacaa aaaagttcac atcaacagtc
     1441 acgttattct ttaattacta aatttttgtt tttaactgga ctacgttatg gtgaattgat
     1501 atctttgact gaatctgatt atgatggtaa taaaattacg gtttctggta cttatgatta
     1561 cgagttaaaa attaaaacaa caactaaaaa tacaggttct tatagaacca ttgaattatc
     1621 aagtaatgct aaagaaatca tagatcaatt aattgaagaa aacaaattaa ttaagaataa
     1681 taaagataaa tacattttca tctctaaaaa tggtaacccg atatctattc aagcatataa
     1741 tcaatcttta agagcagtat ccaaagaatt aaacctagat aaaaaagttt cttctcacat
     1801 gttaaggcat tcccacattt ctctacttac agaattagga atcccattaa aagccattat
     1861 ggatcgtgta ggacatgaag attcaaaaac aacattaaag atttatactc atacaactaa
     1921 aaatatgcaa aatcaactag tagaaaaact aggaaaaata gaaatttaaa ttgccccttt
     1981 tctgcccctt ataaaaacaa acaacataaa atatataaaa agaaccccgt tataacgagg
     2041 ttcttttaat ttaactttca aatacaaatt gattatgata aagttccgca taaaatccat
     2101 tttctgctaa taactcgtga tgactgcctt cttcaatgac ttccccatca cgcaagacaa
     2161 cgattctatc tgcattcaag attgttttca gccggtgagc aatcacaaag ctggttcttc
     2221 ctttgattgc ttcatccatt gctttttgga tccttgcttc tgttaccgtg tctacattac
     2281 ttgttgcttc atccaaaatc aataaagatg gatttgtgat gatcgttcga gcaatactga
     2341 tcaactgctt ttgacctgta ctgaataagt tattttcttc ggatatctct gtatcataac
     2401 ctttttctaa cgtcatgatg aagtcatgaa tattcgcttg ttttgctgca ttgatgactt
     2461 cttcatctga agcgtccggt ttgccgaatg tgatattgtc tttgatcgtt ccggagaaca
     2521 atacagattc ttgcaaaacg attcctacat gtctacgtaa tgcatcaaga tcataatctc
     2581 ggatatctat tccatcgatg actactgaac catcattgac atcgtaaaaa cggtttagca
     2641 gattcatgat cgtcgttttc cccgatcctg ttggaccaac aagagcaacc atttctcctt
     2701 tgtccacatt gatcgagaca tctttcaaga ctagtttttc tggagaataa ccaaaatcta
     2761 cgtgctgcaa cgcaagacct ttttcaacac catcaaatgc tttacagttt tctggtttga
     2821 cttcatcttt ttcatcaaat atttcattca atcgtcttgc ccctgtaatc gctaattgga
     2881 tcatgctgta tccagatgaa atctgcatca gtggttggta atattgttgt gaatactgga
     2941 cgaatgtcac aactaagcct aatgccaccg aacgttccaa atcaccattt aatgctaacc
     3001 agccgccgaa aaagataact atcgctgtat tgaccagtga cattccttgc atcatcggaa
     3061 atagtagacc agaataagct tgtcctttga aggttgcttc gcgaacactt tgattatgct
     3121 ctaagaatcc ttcaatcgtt tcttcttgta agccatttgt gatgatgaca cgttgtccgc
     3181 tgattttttc atccatataa ccatttagtt tccccacttc atcttgctga agatccacat
     3241 atttccgtgc tttgcggata atcaagacag ctatcaaaac ggctactgga gtggaagcaa
     3301 ttgtagccca agctaactct acattttggc ggaacatcat gatcaagatc ccgacaagta
     3361 aaacagcatt cgtgatgact tgaagcaaag cttggttcaa actattttga atattatcta
     3421 agtcactggt aaaacgactt aaaatttcac cgtcttgatg agaatcaaag aaacggatcg
     3481 tcaatttttc caatttgttg aatagaccga tacgcattcg gttcgttgac ttccctacta
     3541 cttgtgtgaa caaaatacta tagataaagt tggctgcact agctagtaca taaaacagca
     3601 agagtttcca tattacagaa acaaatttgc ttttatcgtc tacgccttgc atcaagcctc
     3661 ctacgtaatt agctaattct tgaaaagctt ccccgatgta ttgaggtgcc tttacttgta
     3721 gataggtagc aatgatcact gtgataaaga taaagaaaaa ggagagtttg taccgcttca
     3781 aatagtgata gaaaaattta cttgctttta ttagatcagt catacttact cctccgttcc
     3841 tttttgagtt tcaaagattt cctgatagac acggtttcca gctaccaatt cctcatgggt
     3901 tccttcgccg actaatctgc cttcatccaa tactaagatg cggtctgctt tgacaacaga
     3961 agcaattttt tgtgcgatga caatcgtcgt tgttcccttc aaatctcgat cgagtgcttc
     4021 tcggaccagc ttttcagagc gagcatccag tgcactcgta ctgtcatcta ggatcaagat
     4081 ctttggatta ccgataacgc cacgagtaat ggataaacgt tgcttttgac caccagaaaa
     4141 gttactgcta cgttcttcta ttggtgcttc gtaacgatca gataactttt cgatgaattc
     4201 ttttgcttga gcaatagaag aagcatgttc catctctttt tctgttgcat tctttttccc
     4261 ttggcgcaag ttttgagcaa tcgttcctga gaataaaatt gctttttgca aaacaaagga
     4321 aaccgtttgt cttaaactct tttcatttac ttgacgaaga tctacaccac ctacttcaac
     4381 tttgccttct gtcggatcaa atagacgagg gatcaattgt gctagtgttg acttcccggc
     4441 accagtcgcc ccgacgatac caaccatttc tccaggtttg atagaaaagc tgatatcttt
     4501 aagcgtatct gtatcctcac caggataacg aaaagaaaca tggtcaaaag tgacagaacc
     4561 cgtcaactct tgttctggaa cgtctaaata agtaagatct ggttcagcat ctaaaatttc
     4621 cgcaatccgc ttcagcgata cggctgctct tgaagtcatc atcatcatca ttccgccaat
     4681 aatgattgcc atcataatct gcatcaagta gttcatgaat gaagcaattc caccaattaa
     4741 cgtaggatca tctttaacaa gatcgctcac aaaaaagatc gccccaacca cagccaaatt
     4801 ggcagccagc atgaaggacg ggatcatcac agaaaataat gtaccgacta tgatgttgtg
     4861 ttttgttaag tcctcactta ctttgctgaa tgacttcaat tggttttctt cttgtacaaa
     4921 agatttcact acacgaatcc ctagtaagtt ttcttttgca atgctattca cacgatcaat
     4981 cagtttttga atgatcatga agtgcttgcc catccttgta aatgaaagtg ccgtaatcag
     5041 aaataccgta acgataagcg ctacgatgat ccaccataat tgcggcatcg tgatcatagc
     5101 taaaatgaag gcaccaataa ataagaatgg aattcgaaaa agtgattgca aagaaatcat
     5161 cactaaattt tggatttgcg tgatatcatt tgttagacga acaacaagat ttccagcaga
     5221 gaactgttcg atatttccaa atgaaaatgt ctggatcttt cgaaatgctt cttctcggat
     5281 atctgcactt acgccttgag ctacttttgc agagaaaatc gtgttcgtta cacctgcggc
     5341 taatcctagc aaagccagac tgatcaggta aataccaatt gttttcattt catcattatc
     5401 ttctgtaata atagcttcca atacttgctg tagtaactta ggttgccata atgctgctgc
     5461 aaccatgact gctacggaca gcaaggaaat aaaaacagct actttatatt ttttcgcatg
     5521 tcgtaagatt aatttcattc gttgattaca cccctttgtt taaattgaca ggactaattt
     5581 aacactgtaa aattagaccg actaacaatt taccatgttt aaaaagataa tgcaatacac
     5641 gaggttgata aaatggaaat aaagctttct caagcaatca tcattcaggc cgcctttgat
     5701 atcctggctg aaacacaagc tctttcgcag ctttccatgc gaaacatcgc aaacagaatt
     5761 catgttcaag cacctgcttt atattggtat ttcaaaaaca aacaacagct cttgcaaata
     5821 atggctgaaa caatggaaga agaattagcg cttccagatc caactttgcc ttggaatgaa
     5881 cagttgctgc agtatatgga aaattactat gacctttata cacgtttccc ttgtggggca
     5941 gaattagaaa tcaatactgt gcccgctttt ccgagtcgat tagaacattt ggagcaaatg
     6001 atccaattac ttgttcgtga aggattttca gtcacccaaa gtcatcaggc cattgcctct
     6061 ctccacaatt tattgattgg acagttaatg gatcagcagc atgaacagca gcttcgacaa
     6121 aaaattatgg acggaaatga atttttgaaa gaaacagttc tatttatgcg taattatgta
     6181 gttgaaaatc aattagaggg atttaaagaa agtatgcagt cccatgcaga attgaatgaa
     6241 aaagcactct ttctaacgag tgcacaaacc tatattgaag gcttatgcca acaaaataaa
     6301 agaagagaaa acgagcagta aaaaagctcc tatagagatc actatgtttg tgatctctat
     6361 aggagctatc tttttaagac tcttttttta tttttcttcc attagccacg ccaatgcacg
     6421 ttgaatccat tggtcccaga agatccagtt atgatcgcca ggtccttgtt caaaggtgac
     6481 cgccaagcct tgttcctgca tagcagatac catttcctga tttgcctgat aaagaaaatc
     6541 agattcccca caagccatat aaaatcttgg aactggtttt ccgcttttta ctagttcttt
     6601 caataagtat tgcggatcat tttttgaacc ttcgatttga tccaatggtc caaagatccc
     6661 ttcccaaaaa gatgctttac gaaccatcaa aagatcatcc attcgatttc ctacagaaac
     6721 agcgcctgaa agagagatta ctgctgcgaa attttctgat tttgcaaggc ctagttttaa
     6781 cgcgccatat ccgcccatcg ataatccagc ggcatatgtt ttttctcttt ttcttgttaa
     6841 ttgcgggaat aattcatgac aaatctcagg tagctcttct gcaatgaatg tccaatactt
     6901 catgtcataa actgtatctg tgtaccagcc aagatcagta gatggcatga tgacagctaa
     6961 tccgtagttt gctacataac gttcaatcga cgtacgtctt tcccatacac tatgattgcc
     7021 acccattcca tgaagtaaat ataaaacagg tatatccgtt gtctcttgct ctgaactggt
     7081 gccaatcacc ttttttgttg tttgcggtag tatcacattt agattgactt ccatttctaa
     7141 cacattcgaa taaatatttg cttgaataaa tgccatattt tcgccttcct tagtaagaag
     7201 ttatttttga ataactttaa atttgaatga ttcggctacg tacatatctt aaccgaaact
     7261 actgttttat tttaccactt ttttcatgaa cgaaaagcgt taagaccaaa gcaatgatca
     7321 cgagtaacaa gccaactaga taaacggaat gtaacccatc ataaagaatt tttcttaatg
     7381 gtaacaaaat atccgcatca atatatttag ccgtttgtgg attcaccagt ttattcatga
     7441 tatctgcatc atttgttaaa tttgctgctg caagttctcg tttagttacc ccatttatca
     7501 ataaaccaaa aatagaaacc atgactgttt gaccaattgt tcgaactaac gtattaaaag
     7561 aagttgctac accaagctgg tcacgagata cactattttg tgcactgaca gtcgaggtag
     7621 tcatggtcac acctaagcct ataccgatga ccgttgatat agcaaaaaac cataaatagt
     7681 tacttctgaa aggcataaga tacaaccaag cgcccccaat agctgcgatt agtagtccaa
     7741 ttctcaagac ccaatgagtt ttcatttgag tcatccactt gcttgcaagg aaagaaccga
     7801 ccatccagac tagtgataat ggtgctaata ccagcccacc aattgctgca ttcagcccta
     7861 atacaccttg catccacata gggatataaa catcgattcc catcaaaaaa ccactagcca
     7921 atgctgcagc caagttgacc acaacaaata gtggattttt gaataaatgc atcatgatga
     7981 caggatcttt agctcttttt tcggcaaaaa caaaaatacc taacattacg atactaccta
     8041 gcagcaaagc agaaacaagc ggactcattc cttcgtctcc taataattgg aaccctaaca
     8101 gcaatgagac aagcatggcc atcaaactaa aactaccaaa aatatccatt ggtttagact
     8161 catgtatgat ttttggttca tttaaatata gccaaatcaa aataattaat agaattccga
     8221 tcggcacatt gataaaaaag atccagtgcc agctgatcgt ttcaacgatg aatcctcccg
     8281 caagcggtcc aaaaacactg gcaatccccc aggctgaact attcaaacct aaaacttttg
     8341 ctcgcttttc aatagaataa agatcgccga tgatcgtcaa agctactggc atcattgccc
     8401 cagcacccat cccttgaatc gcacgagcag caatcaaccc aatcatgctt tgagataaac
     8461 cgcaaaataa cgaaccgata ataaacaata atgtcccaaa aataaagatt ggtttccgtc
     8521 ccactttatc cgcaagtttt ccgtaaatcg gagtaagcat agcgtttgtc agcaaataaa
     8581 tcgaaaatac ccagttcatg atttccattc catgtaatga accgacaatc gttggcatag
     8641 cggttgttac gattgtacct tcaatagccg tcatgaacgt tgcaatgaaa atactcactg
     8701 tgaccaaagt aacatttgtt tcttttttca tcttattcct ccctttcatt atgacaggat
     8761 ctaaaaatag ataaataatt actatctttt tcaagaggtt aactgccata aaaaaagctg
     8821 gaacaaccac cacttggcga tcgttccagc tgaatataac ttattttgct aaacttgctt
     8881 tcaacgcatc tactttgtcc gtatgttccc aaggcaaatc tacatctgta cgaccaaaat
     8941 gaccgtaagc agctgtttgt ttgtaaatcg gacgacgcaa atcaagcatc tcgatgattc
     9001 ctgctggccg aagatcgaag ttttctctca ctgcagcaat cagttcttct tccggaaccg
     9061 ttccagtacc gaaagtattg attgagatcg aaacaggttg agcaacacca atcgcataag
     9121 ctagctgtac ttctgctttg cgagcaagcc ctgctgcaac aatgttttta gcaatataac
     9181 gtgcagcata gctggcagaa cggtcaactt ttgtcgcatc tttaccagaa aaagcaccgc
     9241 caccatgacg agcataaccg ccatacgtat caacaatgat ttttcttcct gttaatccgg
     9301 catccccttg aggaccacca atgacaaatc ggccagtcgg attgatataa tatttcgttt
     9361 gatcatctaa taattcagct ggaataactt ccttcactac tttttcgatg acatcatgac
     9421 gaattgtttc attatctact gcatcatcat gctgtgttga aataacgatt gtatctacgc
     9481 gttccggttg tccttgatca tcatattcaa ccgttacttg agattttgca tctggacgta
     9541 aatacgttaa ttcatttgat ttacgcaaat ctgccaagcg tcgtaccaga cgatgactca
     9601 aagcgattgg taaaggcatc aattctggtg tttcatctac agcaaaccca aacattaaac
     9661 cttggtcacc agcaccaatc tcatctaata cgtctttttt atcttcatcc cggatctcaa
     9721 gtgcttcatc aactccttga gcaatatcag gagattgttc atcaatcgca accaatacag
     9781 ctgctgtatc tccatcaaat ccgaattttg cacgtgtata accaatttct tttattgttt
     9841 cccgtacgac tttttgaata tctacatatg ccgtcgtaga tatttctcca aatactaaaa
     9901 caagaccagt tgttacagac gtttcacacg ctactcgtgc tgttggatct tgttttaaaa
     9961 ttgcatccaa gattgcatca ctgatttggt cagcaatttt atctggatgt ccttcagaaa
    10021 cagattctga tgtaaataag tgtctttcta ccattataat gattccccct agttaaattc
    10081 ggttacaagg catctccgtc ctgcggatcc tttcgggaac ttgcaacgaa ccaagtatag
    10141 cagataaaac agttttctgc tcgtattttt tcattttttt gcttaaaaaa tataaaaata
    10201 ttttacatta gaactgtcca aaaaagctta gcacaatcat ccataaagat aactctgcta
    10261 agctttttaa aatcattctt tcagataaaa agaaaaagat actcaaaaag tatcttccat
    10321 gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag ggcggtgtct taaccgcttg
    10381 accaacgggc caataaaata aatgggccta aatggactcg aaccatcgac ctcacgctta
    10441 tcaggcgtgc gctctaacca gctgagctat aggcccataa aaactaaaag cgggtgacga
    10501 gaatcgaact cgcgacaaca gcttggaagg ctgtggtttt accactaaac tacacccgca
    10561 taatggcgcg ggacagaatc gaactgccga cacatggagc ttcaatccat tgctctacca
    10621 actgagctac cgagccaaac ggttccgacg ggaatcgaac ccgcgatctc ctgcgtgaca
    10681 ggcaggcatg ttaaccccta caccacggaa ccaatgtttt tagtttttaa ttgcgggagc
    10741 aggatttgaa cctacgacct tcgggttatg agcccgacga gctaccagac tgctccatcc
    10801 cgcgataata tgaaaaagga ggataaggga ttcgaaccct tgcacggttt tacccgcctg
    10861 acggttttca agaccgttcc cttcagccgg acttgggtaa tcctccatgt aaaactatat
    10921 taaattaatg acccgtacgg gattcgaacc cgtgttaccg ccgtgaaagg gcggtgtctt
    10981 aaccgcttga ccaacgggcc aggtaaaaca aaatattgaa acggagaagg agggatttga
    11041 accctcgcgc cggtttcccg acctacaccc ttagcagggg cgcctcttca gccacttgag
    11101 tacttcccca aaaccaataa gattttgtac taatgggcct aaatggactc gaaccatcga
    11161 cctcacgctt atcaggcgtg cgctctaacc agctgagcta taggcccata aaaaactaaa
    11221 agcgggtgac gagaatcgaa ctcgcgacaa cagcttggaa ggctgtggtt ttaccactaa
    11281 actacacccg catggcggtt ccgacgggaa tcgaacccgc gatctcctgc gtgacaggca
    11341 ggcatgttaa cccctacacc acggaaccag tgtttttagt ttttaattgc gggagcagga
    11401 tttgaaccta cgaccttcgg gttatgagcc cgacgagcta ccagactgct ccatcccgcg
    11461 ataatatgaa aaaggaggat aagggattcg aacccttgca cggttttacc cgcctgacgg
    11521 ttttcaagac cgttcccttc agccggactt gggtaatcct ccatatgaaa cttacaacgc
    11581 cacaatggac cttgtaggac tcgaacctac gaccggacgg ttatgagccg tctgctctaa
    11641 ccaactgagc taaaggtcca ggctcttaaa aatcgcggcg gaggggatcg aacccccgac
    11701 ctcccgggta tgaaccggac gctctagcca gctgagctac accgcgaaaa agatattcat
    11761 tatttaaatg aaatggagcc tagcgggatc gaaccgctga cctcctgcgt gcaaagcagg
    11821 cgctctccca gctgagctaa ggccccgtgt ttaaatttat aattatcggg aagacaggat
    11881 tcgaacctgc gaccccttgg tcccaaacca agtgctctac caagctgagc tacttcccgt
    11941 ttaattaacg cgcccaagag gagtcgaacc cctaaccttt tgatccgtag tcaaacactc
    12001 tatccagttg agctatgggc gcatatttga aatgccgagg accggaatcg aaccggtacg
    12061 gtgatcactc accgcaggat tttaagtcct gtgcgtctgc cagttccgcc accccggcgc
    12121 gattgctttg gcaaagcgga aaacggggtt cgaacccgcg acccccacct tggcaaggtg
    12181 gtgctctacc actgagctat ttccgcgtgt ttaaagatgc cggctaaagg acttgaaccc
    12241 tcgaccctct gattacaaat cagatgctct accaactgag ctaagccggc atgtaaaact
    12301 taaattatgc gggtgaaggg acttgaaccc ccacgccgta aggcgctaga tcctaaatct
    12361 agtgcgtctg ccaattccgc cacacccgca aaaatatgag ccgtacaggg ctcgaacctg
    12421 tgaccctctg attaaaagtc agatgctcta ccaactgagc taacggctca tatggaggtt
    12481 aacgggatcg aaccgctgac cctctgcttg taaggcagat gctctcccag ctgagctaaa
    12541 cctccatgga taactt
//
LOCUS       DAZKLA010000079        12492 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000079 DAZKLA010000000
VERSION     DAZKLA010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.080"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..375
                     /locus_tag="ACG85N_001908"
     CDS             <1..375
                     /locus_tag="ACG85N_001908"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224647.1"
                     /translation="VKDNRNSIRKEVLHLIKFVKKTVAIPSEKKINESTRKLYRRANK
                     KALKLSAFLSSLSLKLSLILFISEHVSITDFLNLAKNPYLKGVRSILWQGLQSLACLE
                     VRTWFSYSSCRKIFLMMILSIN"
     gene            complement(324..893)
                     /locus_tag="ACG85N_001909"
     CDS             complement(324..893)
                     /locus_tag="ACG85N_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0224648.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="ACG85N_001910"
     CDS             complement(967..2256)
                     /locus_tag="ACG85N_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224649.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2514..3458
                     /locus_tag="ACG85N_001911"
     CDS             2514..3458
                     /locus_tag="ACG85N_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0224650.1"
                     /translation="MEEKIEESKQKIDQLIQELEKAVIGKRSTIQLMIVALLAGGHVL
                     FEDIPGVGKTLLVKALAKAVQGTFSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPI
                     FTTILLADEINRTTPRTQSALLEAMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTY
                     PLPEAQLDRFLFRLQIGYPSFDDELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANV
                     FVSPEVASYALQLVSATRSHAAIQLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQD
                     LVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQIPVPVR"
     gene            3472..4545
                     /locus_tag="ACG85N_001912"
     CDS             3472..4545
                     /locus_tag="ACG85N_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFQ0224651.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="ACG85N_001913"
     CDS             4535..6706
                     /locus_tag="ACG85N_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HFQ0224652.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8989)
                     /gene="copB"
                     /locus_tag="ACG85N_001914"
     CDS             complement(6818..8989)
                     /gene="copB"
                     /locus_tag="ACG85N_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFQ0224653.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9009..11195)
                     /locus_tag="ACG85N_001915"
     CDS             complement(9009..11195)
                     /locus_tag="ACG85N_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0224654.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="ACG85N_001916"
     CDS             complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="ACG85N_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFQ0224655.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11417..11857)
                     /locus_tag="ACG85N_001917"
     CDS             complement(11417..11857)
                     /locus_tag="ACG85N_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFQ0224656.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(11931..12429)
                     /locus_tag="ACG85N_001918"
                     /pseudo
     CDS             complement(11931..12429)
                     /locus_tag="ACG85N_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
BASE COUNT         3520 a         2630 c         2217 g         4125 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgttttttct ccatatcgtt cccattcatt tttactccat
     9001 cccttctctt actttttcat gtgccgattt aagctcaggg agttcaacaa tacactgacg
     9061 gaactaaacg ccatcgctcc tcctgcaatg attggattca aaaagcctag tgcggcaaaa
     9121 ggaataccaa tcgtattata gacaaaagcc cagaaaagat tttgtttgat ttttctcaat
     9181 gtcaccctag acaactcgat tgtttgtgca atggatgcca aactgctact catcagcgtc
     9241 acatcggctg tttccatcgc gatatccgtt ccacttccca ttgccatacc aacatcagct
     9301 aatgctaaag ccggtgcatc attgattcca tcgcctgcca taccgacttt tttgccatct
     9361 ttctgaagtt tttctacgta ggctgcttta tcctctggaa gtacttcagc aaagatatgc
     9421 tttggatcaa taccaacttg tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc
     9481 agcataaaaa catctaatcc tttattctgg agctgcttga ttgcttgttt ggcttctagt
     9541 ttgatttgat cagccacggc aatcaacccg atcacttgct tttcatcagc taaaaacatg
     9601 actgtttttc cctgctgttc taattctaac gcgtgttctt ggtattcatc aaatgatagg
     9661 ttcaattcaa ttaatcgttt acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg
     9721 ctgattccag cacctgggtg agctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc
     9781 agttttccgt attccacgat tgctttacct agtggatgtt cagaagcttg ttctaaagta
     9841 taaaaaatag ataggacttc tttagaaccg actagatctg taactttggg ctttccttct
     9901 gtaatcgtcc cagttttatc caaaacaatg ctgtctaagt gagcagctgc ttctaatgct
     9961 tctccgcctt taattaaaat tccatttcgt gcaccaacac ccgttcctac cataatagct
    10021 gttggagtcg ctaaacctaa tgcacatgga cacgcaatga ctaatacaga gacactatgg
    10081 atcaatgcga gttgccaatc accagtgatc aaacctgtag ctatgagcgt gataaacgcg
    10141 atgactaata cgatcggaac aaaaatccct gagatccgat ctgcgatttt ttggattggg
    10201 gctttgctcc cttgagcatc ttccaccatt tgaatgattt gcgctaatac tgtctgcttg
    10261 ccgacttgtg tgacttccat gtgtagcaga ccattcgtat tgattgtccc gccaaaaagc
    10321 gtgtcatccg gattcttttc tacaggaaga ttttcaccag taagcatact ttcatctatc
    10381 gcactgcttc ctgaaataat tcgaccatct gctggaatct gctcaccagg acggatgact
    10441 agttgatcac caacgactac ttcttcaatc gctaacgttt cttctttccc gtttcttatc
    10501 acttgggcgg tttttgtttg aagagacatc aattgtttga ttgcattacc tgttttcgtt
    10561 tttgctgtat gttccaaata tttgccgagc aaaatcagcg tgatgatcat actgctgctt
    10621 tcaaaataaa gatcttgggg gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag
    10681 gcagccgatg ttccaattgc taccaataca tccatatttg gtgctttcga cttgattgca
    10741 tggtacgcac ctttataaaa acgagcaccg acataaaatt ggacaggagc agacagcact
    10801 aactggacga ttggaaaatg aaaaaagtga acaatcgccg catggcttcc taacatcata
    10861 gcaatcattg acaacatcaa cggcaaagtc aaaaccgtac tcaatataag atcgaatagc
    10921 atttttttca aatacgcttg cttttcttct gcaatttttt gtttgtgggc ttcatcgtac
    10981 aaaattgcac catagccgat gttttctacg ctttggatta atttttcagt ggtcgtccct
    11041 tcatatttga cacttgcttt ttcagtggcc aagttgactg tggcattgac cacaccaggt
    11101 tgtccgttca gctctttttc gatcctagca gaacaatttg cacaagtcat acctgtaatg
    11161 acgaacgtat ccattttggt ttctgctgtt gccatcaaat cacctctgct ttataaccca
    11221 atccattgat tgcttgacaa atcttttcgg attgaacttt tgtttcatca aacttgaccg
    11281 taccgtttgc ttttttcaaa tttactttca ctttttgcac gccgtcaagt gcgcttactg
    11341 tttcttctac tcttgctgca caatgattgc agctcatacc ttcgattgaa aattgttgtt
    11401 tcattttttc ttcctcctat tgatgttgtt tacattcaca ttgacctggg atacagttac
    11461 attcgatcgt atctactggt tgcttttgtt ccagttgttt caaaatctca ttgatatctt
    11521 cttgagtcaa agtggcttct tctactagat ccgcaatcgt ctctccaatc ctttttgcac
    11581 agatatggga aaaaagattt tccgtcgcac tgcgaactgt ttccatctcc gagacagctg
    11641 gatgataaat gaattttttt ccttcttgtt ctgtccagat cacttctttt tttaccaaac
    11701 gacccagcaa agttttgatc gttgctggtt tccaatccat cgaatcagct aagatctgcg
    11761 tgatttcttg ggctgtcgtc tgtccaagtg tccaaataac acgcatgatc tcccactctg
    11821 aatcactaat ctttactggc gttttgactt tttccattta tttcacacct ttcgtttaca
    11881 aatgtaatca ttaacttatg aatacagatt acacttgtaa acaaaaactg tcaattattt
    11941 ttgctctcta ttttcaattt agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg
    12001 atcagtttat ttcttgcatt agctacttgg atcagctgct gtatttttgg ttcattgttt
    12061 ccttcaaaat gccagataaa agataaaaat tctaagtatt tcctgtttag tttctccgaa
    12121 aatgattcag gcatatctgg ccgagatttt ttgtttatct ttctttttag agaacgccaa
    12181 actactcggt agatggcgag ataacgcggc actttaagcc aaatgatctt atctgcttct
    12241 ttaatccttt cctctaacgt agaagtataa tttccttcaa cgatccatga cggattagat
    12301 accataaacg attgctgttc ttgcaaaaac cacttttttg cctgaatgcg gaaagccggt
    12361 gatttctttg atcttccttg cgagggacga ttttcccgac ccactttgtc caatcacgat
    12421 gatcttcatt cttctccctc acttctcttt ggaatttggt tcgatcatac cacttataac
    12481 gccgcaaaaa aa
//
LOCUS       DAZKLA010000080        12343 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000080 DAZKLA010000000
VERSION     DAZKLA010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.081"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..154
                     /locus_tag="ACG85N_001919"
     CDS             <1..154
                     /locus_tag="ACG85N_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0224657.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(208..486)
                     /locus_tag="ACG85N_001920"
     CDS             complement(208..486)
                     /locus_tag="ACG85N_001920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224658.1"
                     /translation="MENKVGYVRYNFFSSTPIMKDFASFNEALAVELSHDRERIHYEK
                     HVIINDLWNTEKTDLLALPDTDYPVFKEIEVKANKYNEIKQNKITNLS"
     gene            complement(601..1218)
                     /locus_tag="ACG85N_001921"
     CDS             complement(601..1218)
                     /locus_tag="ACG85N_001921"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224659.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPNKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVT"
     gene            complement(1613..2437)
                     /locus_tag="ACG85N_001922"
     CDS             complement(1613..2437)
                     /locus_tag="ACG85N_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0224660.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            2780..3196
                     /locus_tag="ACG85N_001923"
     CDS             2780..3196
                     /locus_tag="ACG85N_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0224661.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="ACG85N_001924"
     CDS             3193..3663
                     /locus_tag="ACG85N_001924"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0224662.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="ACG85N_001925"
     CDS             3677..4450
                     /locus_tag="ACG85N_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0224663.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="ACG85N_001926"
     CDS             4464..5276
                     /locus_tag="ACG85N_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0224664.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="ACG85N_001927"
     CDS             5330..6295
                     /locus_tag="ACG85N_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0224665.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6431..9154
                     /locus_tag="ACG85N_001928"
     CDS             6431..9154
                     /locus_tag="ACG85N_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0224666.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9167..9799
                     /locus_tag="ACG85N_001929"
     CDS             9167..9799
                     /locus_tag="ACG85N_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0224667.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10007..10231
                     /locus_tag="ACG85N_001930"
     CDS             10007..10231
                     /locus_tag="ACG85N_001930"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFQ0224668.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10333..10566
                     /gene="relB"
                     /locus_tag="ACG85N_001931"
     CDS             10333..10566
                     /gene="relB"
                     /locus_tag="ACG85N_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFQ0224669.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10556..10825
                     /locus_tag="ACG85N_001932"
     CDS             10556..10825
                     /locus_tag="ACG85N_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0224670.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(11004..12305)
                     /locus_tag="ACG85N_001933"
     CDS             complement(11004..12305)
                     /locus_tag="ACG85N_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0224671.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         4032 a         1999 c         2422 g         3890 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcag atgcagcgat taaatgacta tctttatcaa ctaacattgc ttttgaaatc
     6481 tacgccttca gataattatt cagctcgcag tatttcgcaa gctctacagt tgaatagaag
     6541 tacaatcagt agctacttaa atgaaggtgt tcgtgaaggg cttttcataa aagtaaaaag
     6601 ttacccggtt ttatttttac atagaacagc tcttgaagaa ctacatatca cactgaataa
     6661 ctccgaaata gaatcaattg aaagcttgtt aacaatttct caaaaacccg cattagatca
     6721 agtaattggc tctaaaggaa gtttgaaaga agcaattgat caaattaaaa cggcagtctt
     6781 atatcctgga aaagggttgc ctttattatt gatcggcgct agtggatcag ggaaaacctt
     6841 tctagcgaat aaaatatatg aatatgcagt agaagaaaag gtcattcaca catcggcacc
     6901 tttcatagat tataattgtg cacaatatgt gagcaatcca gagttactat cctctgcttt
     6961 atttggctac acgaaaggag catttaccgg agcaagccag gagcatactg gtttgctaga
     7021 aaaagccgat ggaggagtat tgtttttaga tgaagtgcat cgcttatctg aagaagggca
     7081 agagaaatta tttacattta tggatacggg tgaattctct cctatggggg ataatagtat
     7141 tcggaaaaaa gccgatgttc gattagtctt tgcaacaaca gaaaatatat atacgacttt
     7201 cttaccaact tttcttagga gattgccagt tattgttaat ctacctagat ttcaacaacg
     7261 tccgtcgttt gaacgtctgt cattgattga tgaatttttt gtttctgaga gccaaatttt
     7321 agctaaggaa ctatctgttt ctgatgcatt gattcatttc ttaatgaata gtgattttga
     7381 aggtaacgtg gggaatatta aaaatattat caagtatgct tgcggaaacg catatatcca
     7441 ccagaagagt cagcaaacaa ttcaagttaa attattggat atgcctttag agtacagtta
     7501 taaattcaag gagcaactaa ataaacccag aaagaaaaga gcagatcgaa tctactatcc
     7561 agaagaaaca cgacaagtgc aactagaaag aaacaatcag ctttttgttc aattttttga
     7621 ccaattactt actggattct ctgaagtgat tgaaaataac aaggatatta aactattttt
     7681 agaagaaatg gttaccaagg taacacaagt aatggatacg cttatttttc aagaggatta
     7741 cgaaaaagag cagtctttat acgccattct aaactatcat attcgtcaaa gtattgattt
     7801 tatgcgagaa agctacgggt ttgaacaaga tggtaaccgg gtagtcgctt tggccaacta
     7861 tctatatgtc aaggataatc aagtgatttt tgaaaatagt cttcaagaaa agaaaaccaa
     7921 agatcagctt aacgaatttt ttgctaatgt tcttgaaacc ccttattggt atgcaaaaaa
     7981 aatcttaagc cttttatctg agtgtttaga tcaacctgtg tatgaagagg acattttctt
     8041 tattacgcta tatttttata gtttgaatgt cacgaatgtt aaaacaggta ttaagagtat
     8101 tgtgctagct catggttatt ctacagctag tagtatggcg aatgttgcca accgtatgct
     8161 gaataagaac gtttttcaag cttatgatat gcctttagat attacattag agaaaattga
     8221 agcaaaattg attcaattcc ttggagatta tcgaacagat tcaggcttga ttttattggt
     8281 agatatgggt tcactaaacc aactaggcgg tttattagtg aatcatctaa atggaccact
     8341 gttaatcatt gatcatatca atacaccgtt attattggaa gtcggccaac aagtattaaa
     8401 caataaaagt atcaccgaaa taggagcaga aattgaaaca agtcttactt tcaaaaagca
     8461 gttactactt cccaagaaaa agaaaaagaa agccatcatt acttgttgtt atacagggat
     8521 tggtagtgcg acccagattc aggagatttt aacgaaatgc ttgaaagatg cagttagaga
     8581 attagtaatt attccgtatg attacaaaaa gctgttagaa aacaagaatt ttgagactcc
     8641 ttttcaatta tacgatgtca tcatgattgt aggtacagaa gatcccaaaa ttaatcagat
     8701 tccatatatt ggattagatc gcctgattaa tgggggaagc attggcgaat ttgttcatta
     8761 cttggaaaaa gagctgacta ttgaagtgaa cgatttacaa aaagaattaa ttttcaattt
     8821 ttcaattagt aaaattgtcg aaaatttaac cattttggat ccagataagg tgctatcttt
     8881 tgtacaaaaa gcaattaaag aattatcgaa gttattaaat tttgattttt ctaataatca
     8941 gctgtttttg ttgtaccttc actgtagttg tatgattgaa cgaattgtac gtaaagaaag
     9001 tgtggatgag caagaggaca ttgaagaata tatgcaagca aatggagaaa taatggataa
     9061 aattcatagc gctttttttg agattgaaag ggaatacaca attgaaatac cagatttaga
     9121 gttgcgatta ttgagcgata ttgttcaagg ataggagtga attcgaatga caatgggagt
     9181 aatttttgat atggatgggg tactcataga tagcgagaca ttttattttg agagaagaat
     9241 gaacttcttt caagaactgg agatagatcc aggatcaaac cagattgatg attatgtggg
     9301 gaaaacagaa gctgcaatat gggaaacatt agttcctgag gacaaatcat taagagagga
     9361 actttatccg aaatatgtag attatcgaaa aacgcatcca attgatttcg ctaaagcttt
     9421 acgaaaagaa gtgaaaccgt tacttgactt cctcaagaag cgggatatac cattggcact
     9481 ggcatcttct tctccagctg tcgagataga gagtatgctc attcaatgtg aacttactga
     9541 atatttttcc tttgttataa gtggtgagtc attaaaacaa agcaaaccac atcccgaaat
     9601 atatttaaaa gcgaaacagc aattagactg tgacttcttt attgcagtag aggattcaac
     9661 gattggtatt aaatcagcaa aggcagcaaa tatctatacg gcggcactaa aacaaccaat
     9721 gcaaatcgat cagagtgaag cagacataca aattgaaagt ctacaagaac tggaacaaat
     9781 tgttttagaa aaaggttagt gaattaatgg aaataaggct atatatctgt gctagctatt
     9841 ttgactaacc aaaaaaactt gctataattc aggtgtagta aaaggtgctg gtaacacctt
     9901 ttacgaaaat gcttcggcag tgataatggg attggctttt taagtttttt attttgttcg
     9961 caatccctaa ggtcatgtga atgatcctag ggattgctag aatattttgc aacagaacca
    10021 aaatacgaat agttatcaag cagccatatt gactgcggtc aatttaggag gggatactga
    10081 tactactgct gccgtaactg gtgctcttgc gggtattctt tacgggggtg aagtgatccc
    10141 tgaaaattgg agaaaatcgc ttcaaaatga ccagcttctt aaaacgatca gtcagagatt
    10201 ttgcgatacc gtgctatctc aaagaatttg aataatggat atgatgcaaa tcttgtttgt
    10261 actaacgttg tattgacaaa aaggatttta tttagtacaa tgttagtaca aatgatgaac
    10321 ggaggtagaa tcatgtctca atcaaccatt acatttagga ttcccgacga tgaaaaagaa
    10381 ctggtattag aatatgcaaa agcacacaat tcttcattga ccgaacttta tcgaaatgct
    10441 gtactggata aaatcgaaga tgaaatcgat ctacggactt tacggagtgc aattgaagtt
    10501 tccaaagaga aaaaagagag tggtatttct caagaggaaa tggagaaact gctaaatgaa
    10561 ttataaagtc atctacatgc cgcaagccca gaagcaatta aaaaaaatgg acagaaacca
    10621 agctaaaata attgtcgcct ggatcagaaa gaatttagaa aacacgactg acccgcgtca
    10681 gcacggaaaa ggtttgactg gaaataagag tggggaatgg cgctatcgaa ttggaaacta
    10741 tcgaatattg gctaatattt gtgattctga aattgaaatc cagatattct caattggaca
    10801 tagaagtaca atatataaaa gataaccgtt tttggaacga agtagagttt cgaaagtcaa
    10861 tgtataatct tttgaatttt gcataatacc acaggtagaa actacccgat actatcggtt
    10921 ggtttctgct tttttatggc tctttgtcaa ataggactga tagagtgaga taagaacatc
    10981 taaggtagaa ttccttttct actttagatg ttttttgatt cataggtaat cagattattt
    11041 agtaagaaaa ttctggtctt cattttccga aagcttcgaa agccataagc gactcgtttc
    11101 aatgctttga tgtgggtatg cagattttct attttggcat ttgaataagg caattcgagg
    11161 gctaaacgaa tgccggattc atagtttata aaagcttttt ttatagaatg cagttctttt
    11221 ggaacggaat tgggcatcgt tcttacaagt ttaaagaaag tgtccgcctc tttgtcgtga
    11281 aacgcttgaa gaatttcttg atagtaaaaa taggcgattc gtaattcgtc agagatcgaa
    11341 agcagacgat ccaccacttc actttcggtg agatacgccc aaccaaaatt gcgccatttg
    11401 gtccatttgt cataaacaag cttgtcctgt cgtttgatca gaagccgaga caacgccttt
    11461 aattgtcgat aatatttggc ttgtgttgga tcgtttcgat caaagctttt catgattcgt
    11521 acgcgaatat cctcaaattt acgaatgaga tgtttgacaa tgtgaaaacg atccacaacg
    11581 agttttgcct ttggaaaaca ttcctttatc agagaggcat aagaaacatt catgtccgtc
    11641 acgatgacct ttaccttcaa tcgtgcgcgt cgttcaaatt gcataaaata agaaacgaga
    11701 gcctttttct tccgtgaagg aagaatatcc agtaattttc cagtatcgcc atccatacaa
    11761 gaaaaggagt agggaccgac ttgatttttc agtgaacgaa attcatccat taataaacaa
    11821 cgcggtaacc aatttctttt tacttttgtc atggggatca gagaagcgag tgtccgagaa
    11881 acggtcgacc aagaaacgcc gcaatgctta gcaattgtcg tcattgcttg tttttcagtt
    11941 agctcttcta aaattttata gcgcacttgt ttagcgataa agcagtttct atcgacaatg
    12001 taggtattcg ccgtaaaata ggtgtggcag ccacgacagt ggtagcgttg tttcgctaaa
    12061 gcaagataag tcagttggtt cgcacattta ttaagttgaa tgagcgattt tttctttccg
    12121 ttcttaacga tcatgccttc atgattcatc tgtttacaca aaggacagcg acgaatttct
    12181 ttacttaacg tcgcagaaac aacaagactc ttgatattct taatgctctt cgattccaca
    12241 tttgtgatga aaatgttttt atctttgatt ccaagtagta gtttgatatg atgaagatca
    12301 ggcatacaga acgctccttt gattgtgttg tggtgattca att
//
LOCUS       DAZKLA010000081        12195 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000081 DAZKLA010000000
VERSION     DAZKLA010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.082"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(157..321)
                     /locus_tag="ACG85N_001934"
     CDS             complement(157..321)
                     /locus_tag="ACG85N_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224672.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            616..1944
                     /locus_tag="ACG85N_001935"
     CDS             616..1944
                     /locus_tag="ACG85N_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFQ0224673.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2146..2979
                     /locus_tag="ACG85N_001936"
     CDS             2146..2979
                     /locus_tag="ACG85N_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFQ0224674.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2995..3732
                     /locus_tag="ACG85N_001937"
     CDS             2995..3732
                     /locus_tag="ACG85N_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFQ0224675.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3887..4855)
                     /locus_tag="ACG85N_001938"
     CDS             complement(3887..4855)
                     /locus_tag="ACG85N_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFQ0224676.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5087..5920
                     /locus_tag="ACG85N_001939"
     CDS             5087..5920
                     /locus_tag="ACG85N_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFQ0224677.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5954..6793
                     /locus_tag="ACG85N_001940"
     CDS             5954..6793
                     /locus_tag="ACG85N_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0224678.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6819..7379
                     /locus_tag="ACG85N_001941"
     CDS             6819..7379
                     /locus_tag="ACG85N_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0224679.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7578..9299
                     /locus_tag="ACG85N_001942"
     CDS             7578..9299
                     /locus_tag="ACG85N_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFQ0224680.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9464..10606
                     /locus_tag="ACG85N_001943"
     CDS             9464..10606
                     /locus_tag="ACG85N_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0224681.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10622..11833
                     /gene="thiI"
                     /locus_tag="ACG85N_001944"
     CDS             10622..11833
                     /gene="thiI"
                     /locus_tag="ACG85N_001944"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFQ0224682.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4154 a         2048 c         2533 g         3460 t
ORIGIN      
        1 tcgagtccta ctgccggagt aaataaagct tatgcaaaga aaaagaaatc caatcacttg
       61 ggtttctttt tcttttatta ttatgatgaa tcaagctttc tgtttttgtc acgtatgttg
      121 taatctttac gaaagagtag agttggctac tcataatcaa ccatacttcc catcatcgct
      181 aaatacgaaa taaatcagca ttcctacacc aatcaaagca caagctgcac caagaaaagg
      241 gatgacattc ccaaagttat ctgtattcat aagtatgcca ccacccacaa acaaaaagcc
      301 gccagcaatc aggtttttca ttttattcat ctcctttctt acgttatagt atttccttct
      361 ttcaatgagt attttgaaag ctatcttatt gtatgatgtt cttctaggcc tcttctttat
      421 tatacaggcc attttcgtta tctcatagaa atattccttt ttatctgaaa actattgtat
      481 accgtatgac atttttcttc atgaagttgt aagtatattc tcattcaaat gaaaatttca
      541 tcatagaaga gattgtattt ctaaattctt aagagtaaaa tacgaagtat aacttggatt
      601 tggaaggatg attggatgaa agctattacg cctgatatga caacaaaatt taacaaacag
      661 tttcaagcaa atatcaaaca gcaggcattg cagcgggcag ttgtgaagaa cggaattact
      721 gcttctgctg aaaatgtttc agcacatgtg gagaatacac cggtattttc tatagatttg
      781 gctacgggga aagtggctaa tcaaatgcaa tcggggcgtt gctggatgtt tgctgcactg
      841 aacacattcc gtcataagat gctgaatcaa ttccatttaa aagagtttga attatcacaa
      901 aattacactt ttttctggga taaatatgaa aaggcaaatt atttctatga aaacatcatt
      961 gcaacaggaa aagaggcaat cgatagccgg aaagtagctt tcttactcgc tactcctcaa
     1021 caagatggcg gccaatggga tatgatcgtt tcattattcc aaaaatatgg cgttgtgcct
     1081 aaatcggtga tgccggaaag tgcgaatagt tcaaattctc gagatttgaa taactacttg
     1141 aacaaattac tgcgtaaaga tgcagtagtg ttacggaaaa tggttgctca aggagaaaca
     1201 ttagacgcta ttgaagaaaa gaaagaagaa atgctagaaa gcatttataa cttcttagct
     1261 atctcattag gaacacctcc aaaagaattc gattttgagt accgcgatga agaaaagaac
     1321 tatcatcttg accgaggact gacaccacaa attttttatg ataaatacat cggtgtaaaa
     1381 ttagatgatt atgttagcgt gatcaatgca ccaacaaaag acaaaccatt caatcgttct
     1441 tatacagtag agatgctagg aaatgtcgtt ggcgggaaag aagtcaaata tctgaatgtt
     1501 gatatggaaa ctttcaaaaa attagcgatt gctcagcttg aagaaggaga atctgtttgg
     1561 tttggttgtg atgtaggtca atcttctact agaactagtg gcatcatggc cttagatgct
     1621 tattcaatgg atgatctatt tgatacggat tttacaatga ctaaagctga acgtttagat
     1681 tttggagaaa gtctgatgac ccatgcgatg gtattgaccg gtgttgactt agtagacggg
     1741 caatcaacaa aatggaaagt cgaaaatagc tggggcgaaa aagttggaaa gaatggcttc
     1801 tttgttatga gtgatgcctg gatggacgaa tatacgtacc aaatcgttgt aagaaaagat
     1861 ttattgacga aagagcaatt aaatgcgttc aatgaagaac caattgttct atctccgtgg
     1921 gatccaatgg gtgctttagc atagacgagt atttcaatga agagtatgta gtaaagataa
     1981 aaagagctgg cttaagccag ctctttttat ctttaaagtc cattccctgt tttttatggt
     2041 ccctgtgcta taattctaaa taaaggataa ggattggaaa taatcctttt atcattaaaa
     2101 atatgatgac gaagtagaat aaaattggga gtagggggga aagtcatgga tatccaaact
     2161 gttttatctg atctgaaatt aaatggtaaa gtgattccag ttgttggtgg cgatgttaat
     2221 cagacctatc gtataaaaac cgaacatcgt gcttattttt taaaaatcca tccgaatgta
     2281 aaaaaaggat tttttgaagc agaagtagac ggattaaaag aactgtcagc attcgtacgt
     2341 gtaccggata cgtatatgct aggtgaaaca agtgaaggcg cttatttgtt aatggaatgg
     2401 atcgagccgg gaaaagggga tcaacgagat cttgctgcag ctttagctaa tctccatcag
     2461 caaacagcgc cacaatttgg ttttcgtaaa gataactatt tgggtacact tgttcagaaa
     2521 aattcttttg aagaagattg gtggactttt ttctttaaag atcggcttga atcccagatt
     2581 tctttggcag aagaaacgaa tcgatggaat gtacagcgtc aagaaaagta tctgcgattt
     2641 aaagaacgtg tattgaaatc ggttgaacca aaaaagatca ctccacgcct cttgcacgga
     2701 gacctgtgga gtggtaatgt gttctttgac cagcaagggc atccagtttt tgtcgaccca
     2761 gctgtttctt atgggaatcg ggaacaagat attgcaatga gccagttatt cggtggtttc
     2821 cgaccggaat ttttagatgc ttatcaaact atatttccat tagaaaaggg atggaaagac
     2881 cgactgccga tttaccagtt atattatctt ttggcacatt tgaatatgtt tggagaaagt
     2941 tacggttccc aagtagatca attactagaa aatttttaag aggagggcgt gtttttggca
     3001 aaaaaatata ccaaaaagca tgaagtttca tattatgagt gtgacatcaa ccaaacgatg
     3061 acatttcctt cgatgttagg agtagttatc aaaacgtctg aagatcaaag cgaccagttg
     3121 ggaagaggca gtgaattcgt caatagcttt ggacttactt gggtcatcac gaattattcc
     3181 atggagatta atcgtctccc tagagtcggt gaagtaatct cagtaacgac tcaagccatg
     3241 gagtacaata agtttttctg ttatcggaac ttctggatcc atgatgaaga tgggaaagaa
     3301 ttagtcaaaa tagaatctgt tttcgtattg atggattttg tgaatcgaaa aatgagcagt
     3361 gttaatgaag agatcattgc tccttttgag agtgagaaga tcaagaaaat caagcgtcag
     3421 gaaaaaattg aaaagattga ttcgggcttc atgttgccat atcgtgttcg tttttatgat
     3481 atcgatagta atcagcatgt taataatgcg atgtatttta attggatcat tgacgtatta
     3541 ggatatgact ttttgactac gcatgttcca gaaaaagtaa ttattcgatt tgataaagaa
     3601 gtagagtacg ggaatgagat cgaaagccat tttgaacaaa tccctatcga aaatggtgtc
     3661 aaaacaaaac atgaaatccg aatgcaagaa cagctgtatt gtgaagcaaa tattatatgg
     3721 aaaaataaat aaaaaagagg ttgtgacaga agggggcaac ttcaagaaat aagaagaaat
     3781 ttacgaaaat tgtttctcaa atttttgtaa attttcgact tagttttcga agaagctatt
     3841 tttgtcacag cctctctttt taagtttagc tattagataa tgatgtttag ttttggataa
     3901 acgaaatcaa ttcttcaccc gacaaaccat agtttacagc aatcaatagt tttaaacgtg
     3961 ctttttgact attaatactg ttacagaaaa taatccccat tttttctagt tcttttcccc
     4021 cgccttcata atcataaaca ggctctgcga ttccattgaa acaacgagaa acaagtacta
     4081 cagggatttt ttggacaaga agcttttcca aagcagctaa ggcttgtgga gggagattcc
     4141 ccgcacctaa agcttcgatt accaaaccgt ctactttcgt atgggcgatg gcttccaata
     4201 agtccccttg catcccagca tatgctttaa cgatcgggat agtcccatct acagcggaaa
     4261 tgtccagtcg ctttgtttct aaaagttcct gcaagaataa aattcgattc ttagttacta
     4321 agccgatggg acccaatgta ggtgttcgga aagtagcaac atttgtggta tgggtctttg
     4381 tcacgtagcg tgcagaatgg atctcatcgt tcatgaccac caaaaccccc ttatctagcg
     4441 cttcttcaca acttgcgaca cgaatcgcac tttcaaaatt ataaaggccg tcacttccaa
     4501 gttcattact tgaacgcatt gccccagtca aaacaattgg taatttgttg ccaatcgttg
     4561 tatcaagaaa aaaagcagtt tcttctaatg tatctgtacc gtgtgtgatc accactccgg
     4621 atatacctga tgcataagcg gacaagatac gttcttttaa taccaacatg tgagaaggca
     4681 tgatgtgtgg tgagggaaga ttgaagatat cttcaactaa taattctaca tgcttgggga
     4741 ttgtgatttc tgcatctaat aatgggtttg ccacatcagg actaacaccg cccgtgagca
     4801 tgttttcttt catggcgatc gttccgccgg tgtgtagtac aagtattttt ttcattttac
     4861 agacttcctt acgaataatt ttgcatagcc gttaaatctt attgtatact atataaaaga
     4921 gttttgctat cagtgaagaa taacgaatag cctaacttat cataaaaaag ctgaccttat
     4981 taaaatggaa aagactcaac cttgacctcc taatacaatg gagtatctgg ttgagtttct
     5041 tttacaagaa agcagccaag gaaatcaatc acgaaggtgg ccggaaatga tcagaatcgg
     5101 attaacatcg tttaaagaac atgacaaatt aactggtaaa aagaataaca ctctttatga
     5161 atatgcaggg tttctaccct tagtggaatt agatactgcc tattatggaa ttccccgaaa
     5221 gtcctcagtt gagaattgga taagtgaagt acctgaatca tttcgctttg tagtcaaagt
     5281 ctatcaagga atcagcggtc aaggagactg gaagccttat tatcagaccg aagaagagat
     5341 gattgcgcat tttttagatg ttatgtcgcc aatggtagat gctggaaaga tgttttgctt
     5401 tttagtccaa ttcccctcat cattcaaatg tacgaaagaa aatgtccagt acttgcgcaa
     5461 acttagaaag tggtttgagg gttatcctgt ttctatagaa ttgcgggatt attcttggta
     5521 cagcgatgaa tttcttgaac gtacaagaaa ctttatgtct agagccgctt tttctttagc
     5581 catcatcgat gagccacagt tgctgaatac aacgattcct tttgatccat ttgtcaccaa
     5641 tccgaacttt gctctttttc gattccatgg taggaatgcg caaggatggc aagcaaatga
     5701 taaagagtgg cgtaaaaaaa gaacgttata tcgttattca gaaaaagaac tgcaagaact
     5761 agcacatgaa gtcaaagtag ttgattctaa gacaaaagag gtaggtgtga tttttaataa
     5821 taattctggt ggtgatgcgg ctgaaaatgc attagcactg caaaaaatat taaatatcaa
     5881 gccagatgga ctcaatccaa cccaaatgga tctgttttaa gggcttattt tattcaagaa
     5941 aggggcgaag tctatgatca aagctatttt ttttgatatt gacggaacat tggtcaccaa
     6001 tcgttcaaaa gcgttggaat caacaaaaca agcaattgaa tatgctcgga aaaacggcat
     6061 tttatgcggt gtagcaacgg ggcgcagccc agtaaagatc aaagaaatca ttgatgaatt
     6121 ggaattagat atgtatgtcg tgtataatgg tcaactggtt tttactgcag atcgcacgat
     6181 tatcgaccac ccttttgaac aaaaagtatt ggaacatatt gttgaatttg cagatgagaa
     6241 ccatcgacaa atcgtttttg gtgcgagaaa tcggttagat ggatcaacga cgatgctatt
     6301 aggacaatcg atttttatca agcgactagt cagcttttta ccgcgtaaat tcccggtacg
     6361 tctgatgaaa aaaatcctcc aggtttttag ccctcaccga caaaaggatc gttatgaaac
     6421 gttagagata ttaaaagaac caatttatca atgtatattg ctcagtccgg agtcagaaca
     6481 gaaaaagtta gaacatcgct ttcccgagtg tacgtttcag cgttcaaata cgtatactgt
     6541 agatatcatc ccaaaaggtg gttcaaaatt actaggcatc caagctttcg caaatgctgt
     6601 cgaaattgaa atggaagaaa ttatggcatt cggcgatcat tacaatgata tcgagatgct
     6661 gaaaggtgta ggaattggag tggccatggg gaatgctcag atcgaagtaa aacatgctgc
     6721 agactttgtg actcaatcaa atgaagaaga tgggatatac gaaggactga aacacttcgg
     6781 tctgatttat tagtcaatga aagaaggaaa aaatcagtat ggaagcagag acaccttttc
     6841 aaaaagtaga aacattccac aaaacttttg atccaagaag accagagata cccacggctt
     6901 ttacagaaaa tgaagctcat acacgtgctg cgttcaaaat agaagaactt gttgaatttg
     6961 ttgcagctac ttcacctaat aaagaacaac tgcatgaagc tgttatgctg ctgcacgaag
     7021 cattagataa agcagaagaa aaaatacttg gtaaaacaca gtggggaagt gatccgctgg
     7081 ttgaacaagt agatgcactg acagatatcc tttactttac ttatggttct ttttcattac
     7141 taggaatagt gcctgatcga atattcgaaa tcgtccatca ggcaaatatg gggaaattat
     7201 ttccagatgg aaaacctcat tatgaccctg taacaaataa agtgttgaaa cctgaaaact
     7261 ggcagatgga atatgctcca gaacctaaaa tcgcggcaga aatccaacgt cagattcaag
     7321 aagcgattgc taagaagaaa tccgctgata taggcatgtg cacaaatgaa aaagagtgat
     7381 cagagtttca ctatttttgt tatctttata cgatggaaaa acaaaagatt ttcgtttaag
     7441 ttagtgaatg catgaaaata gtatgctata atctagaata gatgtctttt gcacgagaga
     7501 cgtattattt ggatttatgc caagcagaac gccatctgtt gcataaaaga tgcatactga
     7561 aaacggagga accataaatg ggaaataatg ttatcatcgg aatcgtcatt gcaatcatta
     7621 tagtcgccat tgtcgtcttt ttggttagtt acttgatgaa gaaaaaaaac caagatcgat
     7681 tggatgcctt agaaaaacgc aaggaagaat tatttgactt gccagtgatt gaagaagtcg
     7741 atgatgttaa aaaaatgcat ctggttgggc aaagccaaaa tacctttcgt gaatggagtc
     7801 aaaaatggaa tgacatatct acttcctcgt tcgctgaact agaaagtcaa attttcgaag
     7861 tggaaaacct caatgaaagt tttcggttct taaaaggaaa aaaagcagtt gttgaagcag
     7921 aagctacaat ggaccaaatg gagaaacaag tgacggaaat ccgtgaaggt ctaaaggaat
     7981 tgcgggaaac ggaagaacgc aactcactag ctgttcagca ggcacttgac gtctatgaag
     8041 aactgaaaga aaaagtcaga gaagacgagc atgaatacgg accagctctt tttgaattgc
     8101 aaaaacagat caaagtgatc gaaaaagaat ttacgcagtt tgtgacttta aacacttctg
     8161 gagaccctgt tgaagcccgc gaggttttgg ataaagcaga aaaacatacg tatgaagtgg
     8221 aagctctgat gaaaaaaatt ccaccgctgt atgaggagct ccataccact ttccctgaac
     8281 aacttgaaga aatcagtgat acttacgaga aattgatgga agaacaatat gtattcccag
     8341 aagaagatct tgctgaagat atcgcaaaag taagtcgaag aattgaaaat tcattagcca
     8401 atcttgaaaa aacagaagtt gaaacggtcg aatttgaaaa ccgtgaaaca gctgatttga
     8461 ttgattcgtt atatgatatc ttagaacggg aaatggaagc tcagagatat gtaaaaacta
     8521 accaatcaac gattgcagag tatatcaaac atacaacaaa gaataaccgt caattattga
     8581 ttgagctgga tcacacatct caaagctata cattgaacca caatgaaatt ggccgtgtac
     8641 gaggtttcca aaccgaagtg gaagaaatgg aacgtcaaaa tgaacagatg attccacaga
     8701 tccgtcaaca cgaaattcct tattcagaag tgcgcacgtt ctataaaaca gtcttcaaag
     8761 tcttagaaga tatcgaaaag caacaagtag agatcgacga ctcattacat gaattgagaa
     8821 aaggcgaaaa agaagcgcaa gaaaagatcg atacattcga attcaaattg cgcagtttga
     8881 aacgttttgt agaaaaacaa cgtttgcctg gcttgccgaa tgattaccta gaattctttt
     8941 ttgttgctac tgaccgtatt gaagaactaa gtgttgtctt gaataaaatt cgagtaaaca
     9001 tggaagaggt caatcgttta gttgctctat gtgaagaaga tctagaatta ttagataaga
     9061 agacacatga tttagtagat gcagcagcat taacggagca aatgctccaa tatgcaaatc
     9121 gttatcgtca tacacacgaa gaagtgagag aagcaattga taaaagctac tatttattca
     9181 ataaagaata tcactatcaa gaagcattag acgaaatcgg tacagcatta gagcgagtag
     9241 aacctggtgc tttcaaacgc atcgaagatt tttattttaa tcatccagac ttagtataaa
     9301 gcaaaaaagt tagtaaacca gcaagagcaa gacggattat tgtcttgttc ttgtcttttt
     9361 ttgtatattt gaaaattgaa acaagtgaaa atttcacctg ctcttgaaag caccttggac
     9421 aaccgttata atagaaaaga atttcaaaaa agaggggttt cttatgatat attttgataa
     9481 cagtgcaaca accccaatct ttccgcaaag cttagatgca tatgtaaaaa ccagtcagcg
     9541 gattattggg aatccatcaa gcttgcatga tttaggcaat caagctaacc ggttgttgca
     9601 gcaagcaaga aagcagatcg ctgatttgat ccatgtagaa ccagaagaaa tctattttac
     9661 ttcaggcgga acagaaggag ataactgggt attaaaagga acgatgatcg aaaaaagaga
     9721 atatgggaat catatgatca tttcaggagt cgaacatccg gccgtcagcg agacagcaga
     9781 acaattaaaa gaattaggaa tcgaagtaag tatcgcccca gtggataaac gcggatttgt
     9841 acgtgtcgac gaattgaaag aattaataag aaaagagaca gtattagtct ctgttatggc
     9901 tgtgaataat gaagtaggat cgattcagcc catccaggaa atcagtgatc ttttggatga
     9961 ttttcctaaa attcatttcc atgtagatgc tgttcaggcg attggaaaag taccattctc
    10021 gcaatggttg acagaaagag ttgattttgc aacattttca gctcataaat tccatggccc
    10081 acggggaact ggtttcattt attggaaaaa gggtcgtcgt ttagcaccat tactgacagg
    10141 tggagggcaa gaaagaaatc agcgaagtgg gacagaaaat gttccaggta ttgtggctat
    10201 ggttaaagct ttacgtttat cattagagat aaaggaacaa aaacctgagc agacacaacg
    10261 cttaaaagta gctctaatca atgcgttgaa gacgtatgag aaagtgacga ttttttcaga
    10321 aggaactgac tatgcaccgc atattttatg ttttgctcta aaaggaattc gcggcgaagt
    10381 tctcgtccat gccttagaag aaaaacaaat atttgtttct actactagtg cctgctcctc
    10441 acgtaaacat atggcaagca gtacattgca cgcaatgcat gtaccaaatg atctggctac
    10501 atctgctgtt cgaatcagct tagatgaaaa caattcgctt gcagaagtcg agcaatttat
    10561 gattattttc aagcagcttt ataagaaatt ttctgcaatc aattaaaaga ggagtgttct
    10621 tgtgaaatac agtgaaatta tggtccgtta tggggaactt tcgacaaaag gaaagaatcg
    10681 aaaaagcttt atcatgcaac tagcacaaaa tgtcagacaa gctttgaaag actttccaga
    10741 aatcaaaatc catgctgatc gggaccggat gcatctttta ttgaatggcg cagacgggca
    10801 gctagttatt ccaaaactag caaaaatatt tgggatccaa aatttctctc caagtattcg
    10861 tgtagaaaaa gatgtcgaag ttttgaaaca agctgttcaa gacatcatga aagaaattta
    10921 tactgggaaa gaaacattta aaattatcgc taaaagaagc gatcaccaat ttgaattgac
    10981 aagtaatgaa ttgaatcaaa cattaggtaa tgctgttttt gatattttcc cgcatatcca
    11041 agttcaaatg cgtcaaccag atattccatt acgtgtagag attcggcgtg atggcgctta
    11101 tctatcgtat gaaacgatca agggagctgg aggattgccc gtaggaacaa gcggaaaagg
    11161 aatgcttatg ctttctgggg ggattgattc tcctgtagca gggtactttg ccatgaagcg
    11221 gggagtggaa attgaagctg ttcattttgc tagtccgccg tatacaagtg aacaagcact
    11281 gcaaaaagca aaagatctaa cagccaaact agcaccttat gtgggaacga tccattttat
    11341 cgaagtgcca tttaccgaga ttcaagaaga aatcaaaaag tcagtgccac aaggatattg
    11401 gatgacaatt actcgtcgaa tgatgttgcg tttgaccgat gaaattcgcc gtattcgtca
    11461 tggtttagtc attttgaatg gtgaatcatt aggtcaagtg gcttcacaaa ctttgcaaag
    11521 tatggtggct atcaatgaag taacgaatac gccaatcatt cggcctgtag cgacaatgga
    11581 taaacttgaa atcattgaag tagcagaaca gatcgataca tttaatcttt ctatccagcc
    11641 ttttgaagat tgctgtacga tttttgctcc tccacagcca aaaacacgcc ctcaactgga
    11701 aaaggtccat caatatgaag agcgtttagc aatagatgaa atgatccaac gagcactagc
    11761 tggcttgaag atggaagaga tacaagcaga atcgtctaat gaaaaaactg aagaattttc
    11821 agaattgcta taagaggtta tgaaaatagt gttttgacca gagtattaga gaaacaaaac
    11881 gaaaaaatag ctccatgtat tttaccgttt ttgaactaat gtcgaagatt agctgattga
    11941 ataccattta tcaagaagtt atggaaaagc cggttctttt ttactaactg aaaaataaaa
    12001 ttttatttag agatcaagct acagttgata aatgatataa aaaagagaat ttcccatgaa
    12061 atagcgaggt tgtgacagaa gtgggcagct tcaagaaata agaaggaatt tacgaaaatt
    12121 gcttttcaaa tttttgtgaa atttcggctt atttttcgaa gaagttactt ctggaacacc
    12181 gtgtatcaga ggttg
//
LOCUS       DAZKLA010000082        12185 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000082 DAZKLA010000000
VERSION     DAZKLA010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.083"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(366..1484)
                     /locus_tag="ACG85N_001945"
     CDS             complement(366..1484)
                     /locus_tag="ACG85N_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224683.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1717..1839
                     /locus_tag="ACG85N_001946"
     CDS             1717..1839
                     /locus_tag="ACG85N_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224684.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            1955..3262
                     /locus_tag="ACG85N_001947"
     CDS             1955..3262
                     /locus_tag="ACG85N_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFQ0224685.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3381..4580
                     /locus_tag="ACG85N_001948"
     CDS             3381..4580
                     /locus_tag="ACG85N_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0224686.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4607..5875)
                     /locus_tag="ACG85N_001949"
     CDS             complement(4607..5875)
                     /locus_tag="ACG85N_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFQ0224687.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(5930..5996)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(6038..6684)
                     /locus_tag="ACG85N_001950"
                     /pseudo
     CDS             complement(6038..6684)
                     /locus_tag="ACG85N_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7092..7595)
                     /locus_tag="ACG85N_001951"
     CDS             complement(7092..7595)
                     /locus_tag="ACG85N_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0224688.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7718..8482)
                     /locus_tag="ACG85N_001952"
     CDS             complement(7718..8482)
                     /locus_tag="ACG85N_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFQ0224689.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8590..8865
                     /locus_tag="ACG85N_001953"
     CDS             8590..8865
                     /locus_tag="ACG85N_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFQ0224690.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9303..10253
                     /gene="yidC"
                     /locus_tag="ACG85N_001954"
     CDS             9303..10253
                     /gene="yidC"
                     /locus_tag="ACG85N_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFQ0224691.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10432..10611)
                     /locus_tag="ACG85N_001955"
                     /pseudo
     CDS             complement(10432..10611)
                     /locus_tag="ACG85N_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11437..>12185
                     /locus_tag="ACG85N_001956"
     CDS             11437..>12185
                     /locus_tag="ACG85N_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFQ0224692.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT         3708 a         2537 c         2155 g         3785 t
ORIGIN      
        1 tttaaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttca caaaaatttg
       61 agaaataatt tttgtcaatc tcttcttatt tcttgaagct gaccacttct gtcacaacct
      121 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccacca aacaaaaaat
      181 atgaagagct atttttgctt gctgttctta ttacttgatg cagaacagct ttttcacaac
      241 ctctcataca cggtgtttaa tcagctgacc ttcggtatta gctcaaaaaa cggaaaaata
      301 tgtgaaacta tttttccgtt tgtttctcta atactcaggt caaaacactg atttcacaac
      361 ctcttttaat aacgaagaat cgctgtcaga ctcttttcat ctggcgcatc tttttgatcc
      421 aaaataaaca ctttcccgcc attaatgatc acatcatctg ctaatgcatt cagcacttga
      481 cgccgatcat attctgtttc aggatcttca ccgaaaccat cagctaagtt tgacgtagcg
      541 ataaataaat gtgacacttt tccttctttc gcagctggaa cgatatcaac taactgatcg
      601 atgaatttct tatctaagag tttattgtat tcttttgatt cttgagctgc gagttcactt
      661 gacagcttct tcgccccttc ttcgatatct ctaaaggata gctgcgcagg agaagcagaa
      721 atcgcaacgt ctgcacaata atagggattt ttagcaattt ttttgaacat cgtctgattt
      781 tctggtaatg caaataaata aatcggccat tgttcaggat tttcgaaaga atccttcaag
      841 tacgtatcaa cagcttggta atagttgacc caatcgatct ctacttcttc atcttttgga
      901 tttacgccat ggaaagttac tccatctcgg gctccgcctc ctccattaga agagaagttc
      961 aaaccgccac ctgtcagttc agatcccaat gcagtaacga catcaaccgg tgccccttct
     1021 ggtagagata cttcttttac ttgtttgttg ctgacctcat acaatttcat ggagtcgcgg
     1081 ttcaatccca ataaataata agaatagttg aactgcccat ttttgataat agccagtaaa
     1141 taaggaagat cacttacata gtattgatcg tctaccggga tactcaaacg atggatatag
     1201 gtctggtcag cagttataat gaccgctaca ctcgtagttg cactacgcca aaacgaagga
     1261 tctcctaaca aattttcaat tttttcttgg aacggggacc agtcatgttc ggaatatttt
     1321 ttctcaaaac gtttctttgc ttctttcgca aaatttttga atttgatctg atctttttcc
     1381 acatcttgat gggcgacatg cgtattcagc atgatcgtga cgaatggtcc tttagcgcct
     1441 tctgaagtca attgtgtcaa taattcttta tccttttttg ccataaacgt acccctccta
     1501 aaaaaatcat tcctttgttt ctatctaaag tctaccacaa cactattttt ccatacaata
     1561 aaacgtatct aaaaaaataa aacggtttct acggttttaa atttagctat ttttttaagt
     1621 tatcttcata atttcctcaa ttatttagcc ccagtcttca actattcttc atgaaatgct
     1681 gataaactaa gaaaaagaat aaaagaggtg gatcttatgc atttgattat gattttcagt
     1741 ttattaagta ttatcagttt atttgctttc agcgcttccc gcttattaga tgcggaaaca
     1801 cagcctattc ctgtaaaagt acgtaaaaac gaccgctaaa cttccataac gcgcgttctt
     1861 atcctaaagt tttttcagaa acattgaaac tctctcccct ctctgataaa attttaattt
     1921 gtagcgaaac aaattaatta caaggagatc ggatatgtcc ataaaaaaaa cacgtctagt
     1981 cctattattg gctagtctca ttctttgcct tgcttctttt ctaccagctg ttgctgtgca
     2041 agctgaagat acgttcaaag taaatgcaaa agcagctttt gctgtagatg cgcaaagcgg
     2101 taagattctt tatgatcaag atggagaaaa aaccatgggg atcgcttcaa tcacaaaaat
     2161 catcggtctt tacatcgtcc ttgatcaagt aaaagaagga aaactgtcat ggggtgataa
     2221 agtttcgatt tcagattatg cagaaaattt gagtatcacc ccagatttat cgaatgttcc
     2281 tttgcataaa gaaaacacct acactgtcaa agaattattc gactctgcaa tcatccagtc
     2341 agccaatgct tcaatggtgg ccttggcaga aaaaatctct ggcagcgaag ctaaattcac
     2401 tgaacgaatg aaagaacaat tgaaagattg gggaatcaaa gatgcaacaa tcgttaatgc
     2461 atcaggattg aacaactctt atcttggcga aaaccgacca gaaggcactg gagaaaacga
     2521 cgaaaatcaa atgtctgctc aagatgtggc catcgttgcc cgccatttga tcttggattt
     2581 cccagaaata ctggatgtat caagtactac gacccaaatg tttggcgaaa atacgcaatc
     2641 accagtggaa atggtcaact ggaactggat gctcccagga ttcgttaatt acaaggaagg
     2701 cgtggacgga ttaaaaactg gtacgactga gcttgcaggt gcttgtttcg ttggtacgat
     2761 cactaaaaac gatcaacgga tcatcactgt cgtattgaat gcggacggcc atgcggaaaa
     2821 cccgagtgcc cgcttcaatg aaacagaccg tttgatggac tactgctatg acaattggtc
     2881 agaaaaagag ctcggaaaag ctaacgcaag tattcctagt ctaaaaacca ttgatgtaaa
     2941 agacggaaaa gaatcaacag ttcctgttgt gcttaaaagc cctgtaaaag tatgggtacg
     3001 aaacgatatg gatactggca aattaacgat cacgccaaca cttgacaaga agcaagtcac
     3061 tgacaatgag atccaagcac ctatggataa aggtacaaaa atcggtaccg ctacgatcac
     3121 cttggcagac gataaattag gctatttaga agacaacaat gcaccatcaa cagagatcat
     3181 tacggataaa acagtggaaa aagcgaatat attcgtgatt ggctggcgac gcgtcgcaga
     3241 atttttcggc aacttgtttt aattgaacaa taagaaaaac atctttccat cggaaagatg
     3301 tttttttgct ttttctagtt taaataacag cttttttcat ctattttcgt tatactgtat
     3361 aagaaaaagg agttgaattt atgaatctaa ccagagatct gatcttttta gttctctatc
     3421 ttattggggt attctctatc gtttattggc gaaaacagaa gaaggatcct tccttgttct
     3481 tttacggcag tacgtggaca tttacggtaa tcttaactta ttttgtcgtg ttggaaattt
     3541 tttattcgac cttttatttt ttgattccat tgttgttctt tagtatcttt gctttttctt
     3601 atttcacaga aaaacgaagg ctactgaatg gtcttttatt caatgtttta ctcatcagtt
     3661 tcggcatcta cttgtttgtc ttgctgtatg aaacgcaaaa tatttttctt ggcggactga
     3721 tcgccttgat cacgattcca ttgctacttg tccttttatt tggtatttat ggattgattg
     3781 tttttctttt ttggaacggg gtcacagttt tacgtagaga atcgcattca ttagctaatc
     3841 ttttaacgtt gatactagcc atttttctta cactgtttct tgtttttgac tttttcttgt
     3901 taaaatattt gccgcaatgg ataaatgctt tgtttttctg cgttcccctg atcttgatct
     3961 atctatttat cgtattctac aactttttga ctgtctcatt cttgtatcag ttcaaccggc
     4021 ctcgatacaa tcaagacttc atcgttgtgt taggcgctgg attgatcaat ggagaaacag
     4081 tttctcctct tctagccaag cgaatcaata aggcaattgc tttttatcgg gctcaaagcc
     4141 gtgctacatt aaatccgccc atcttattga tgtctggcgg gcaaggagca gacgaaaaag
     4201 taccagaggc cattgcgatg aaacaatatg ccatggaaca aggaatccct gaaagagaca
     4261 ttcttgtcga aaccaattcg acaaccactt tggaaaacat gctctattca aaggaaatca
     4321 tggatcagca aatgaaaggg ccataccgcg cgatctttag ttccaacaat taccacattt
     4381 ttagagccgg tctttatgcg cggcaagcca aattgaaagc aaatggtatt ggtgcaaaaa
     4441 cagctttcta ctacttgcct aatgcgtttt taagagaata cctcgccatc ttgatgatga
     4501 acaaaaaaag acatatgatc atttgcggat tgcttttcgc aggctccgca ttcttatcgc
     4561 tgattacttt acttttctaa aaaaacaagg tgtgacaaaa catatgtcac accttgtttt
     4621 tttctttcat tctcttaact attggcaaaa agagtatccc ttttttgacc gctacttgct
     4681 gagcagaata aataccgctg ttgcctgaac acataaagct gatcaagcag atcataaaga
     4741 agtatactgc cgcttgtgaa ccgaacaatt cgatccccat cacaaagcag gcaatcggtg
     4801 tatttgtcgc accagaaaat actccgataa agcccaatcc tgcaagaaaa ggaacagaaa
     4861 catggagcaa aggcgctagt gctgcaccaa gcgttgcacc aatttcaaac aatggtgtca
     4921 cttctccccc ttgatatccc gcgcctaaag acaaaactgt gaagaataac ttcccaataa
     4981 aatcatagac atgtgcctgt ccattaaatg attcttgaag taacggaaga ctcaatccga
     5041 gataacgttg tgtttgcaaa atcaatgctg ctgccacaac tacagctcca cctatcgtat
     5101 ttcgacaaac aggatgagga atccattgtg catatcgcgc ttttaaaaag acgatcgaac
     5161 ggctgaatac ccaaccaatc aaaccaaaag caatcccagc aacaaaaact ttgaagaaca
     5221 gcaacatact ccattcagga atcacaccca tttggtaatg tagatgagaa acaccatagc
     5281 tttctgtcat gaaatttgca aacagtgcag caaagaagct agggaagatc gcttctgttc
     5341 ggactttccc gattgccaac acttctaatc caaataatgt tccagctaaa ggtgtgccaa
     5401 atacagaact aaaacccgca ctgattccac tgataatcaa gatttcccgt tctgatttat
     5461 ccaatcggaa caaacgtgcc gtattatctg ctaacgctcc ccccatttgg actgctgttc
     5521 cttcacggcc aacagatccc ccaaataaat gggtagttat ggttccaaac agtgtaagtg
     5581 gaataagccg caacgggatt ttttcttctc caccatttcc ttgatcgatc actaagttgt
     5641 ttccacgact agaaagtccg ccgtgatacg catagagata agcaaaaagc gcaccactaa
     5701 ctggtagtag acaaaggagc caaggatgtg atagtcggat atctgtcaca actgtcaggc
     5761 tatttaagaa aaaagcagac aagctcccca tgaatatacc gagaatcgct gtgatcacca
     5821 gccacttcag catatacact aaaatgcgta ctggactcgg taattgtttt tccataaaaa
     5881 attcctcctc aataaaaaaa cgcctactaa agaaaggggc tgtccccaga ctttagtagg
     5941 cgtcattagc tatctttcgt tagcatttaa aggcgaacac catcacctat tcacttccat
     6001 taaaagatag catatttttc tgtaaaaaac aataaaatta tcgttctttc tttacacgtt
     6061 cagcaaattc atagaaagga gccaatttcg ttaactgttg attacattgg tcactatcat
     6121 gacaaatata attccccttt ttcgtatacg tgccatcaga acctgctttc gttgtagaaa
     6181 gaaaaagcga aacattcgat gttttctgac agattgcaca gattccctta tttatcgttg
     6241 gtgataaagt accgctaatc ccttttaatt tttcatcttc gtaatagacc atgaattttt
     6301 tctgcgtacc tggatcattc cagccgatgt acgtatgttc ttgcaagtcg atttcattcc
     6361 aagctggttg tttgagtttt ttcacttttc ggaataattt ctcgatttgt ggtttgctag
     6421 gctgcataaa cggctgaacg atttgcttca gctcctccaa tgctctttct gcttgcgctt
     6481 ttgtcagttt tacgtctaaa actgtcgtta aaaatgtatc gattctggca gttcgtgatc
     6541 taatatgtct cgaatctttt cagctgctag tgcctgaact gtttgtaccg tttttggatc
     6601 attgacagat gagtacgcat ttaataactg ttctacttct cttattataa aaaaatattg
     6661 gtatggtttt attgtttgtt ccatgatttt aaagtccttt cttctaacca aagtgcatac
     6721 tttgtagaaa aaagtgatct tctgcaaagt atgtgttacc tgaagatccc ggactctttt
     6781 aaataagata cgttttgcat cttatcatca ccccctttaa tgcttattat tctatgtaca
     6841 tgattcattc gataaataaa agtaaaccgt atcttgaaca gatagtcaac tgcaaacaga
     6901 aaaaaggact gtgacaaaag tcgtgtcaaa gtcccttgtt tcgaatgaat ggtgttcaaa
     6961 agctggcatc tgcggtaata agccgaaact tcacaaaaat ttgagaagca attttcgtaa
     7021 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcaatatg gtctattatt
     7081 ggttttatct attaaatcga ggattctttc ccaaagtaac gttgccatct cttacgcata
     7141 cttgctgaca taggctgagc ggcttctttg actgcgcagt atttgtctac gaatgttacg
     7201 atatatcctt ctttgtactt aggcggtgca atcgttgcgc cccacatgtg cttcaaaatg
     7261 atatctcgct caagatccga caattcggtg atcttttctg cattctttac agcaattctt
     7321 ggatggatat acgcatgtgt gccttcatca aatttcgtcg tacgccaatc atagtagaac
     7381 agatcatgca aaagtccagc acgcgctgtt gctttagcat tgccacccca tttttttgcg
     7441 agcagataac tatagtaaga aacactgatg gaatgctcca aacgagtgga atgaacatgc
     7501 tgtgtgtaat tcgctaattt tttcactgct tctgtttcta aaagatcttc tacataagac
     7561 atatattctt cgtcttcacg ccatttttct gtcataatta tcgatctgcc acctttttga
     7621 atttcatttt cttctgaaat tgatctaagt ataccaccag tgttcggatt attctagtcg
     7681 ccttggataa aatagccaaa gcttaatagt tccttaatca taaagaaaaa tgataaagaa
     7741 caatccctgc cgcaacccca acattcaacg attccgcatt tcctttcatc gggatatata
     7801 cgttctgatc agtcaaagac aagatttcct gacttacccc ctgaccttcg ttccccatga
     7861 ttattgcgaa gttatctgat ttttctactt tgtctaatgc gtgtgcttct tcatttaatt
     7921 ctgtgccata cactgtaaac cctttgtctt tcattaaaga aatcacttct gtcaaatctt
     7981 tttgcaacac aggcaaatga taattgcttc cctgcatgct gcgcagcacc tttgtactat
     8041 aaatatctgc cgttcctttg cctaataaaa caccagccat atcaaatgca tctgccgtac
     8101 ggatcatcgt ccctacattt ccaggatctt gtacattatc taacaacagc catcctcctg
     8161 aaaaatctgg cgtgccttca gccgttaacg ccatcactgc taagattcct tgtggtgtag
     8221 gtagatccga tacagcatcc atgacctcat ccgaaactag aaaagtagaa atctctttgt
     8281 ttgcggccag ccagtctccc cattctttca tgcctcgctg tgtcacaaaa agttctttta
     8341 tttctgcatc agacgacgca gcttcttcaa tcagatggaa cccttccaaa agatattctt
     8401 ccttttcttc ccgatatttc tttttatgta gtttttttaa ttctttgatt cttggatttt
     8461 ttgctgattg gatctctttc acattcctca cctccccatg attatatcat ggaataggtg
     8521 cttgagcttg tttcatccta aaaaaatcgg tatcattaat ttataaaata ggaaagaaag
     8581 gtgacgaaca tgcgcaaagt taaaatgaac gtccaaggac gcgtccaagg cgtaggattt
     8641 cgctacatga ccaagatggt cgctgatcaa ctaggcgtga ctggaacggt aaaaaacgaa
     8701 gatgacggct cagtcacgat cgaagcaatc ggcaatgacg acatcatcca gaaatttatt
     8761 gaagaagtca agaaatctcc gagcccaagt ggacgagtgc agtatgtgga tgtccaagaa
     8821 gacccgatga ttgaggaacg aaagaaattt gatgtggtgg gataaggttg tgaaaaagct
     8881 ggtctgcatc aagcattaag gacaggttgt gaaaaatagc tctccatatt tttcaacagg
     8941 ctggacttaa tgccgtagat gcaagctttt gaacaccgtc gataaggtta ttcttgaggc
     9001 gctttgcctc aaaaattaag gacagcagtg aaaaagctga tctatcactt gcagtgagtt
     9061 catttgactc cgctttttcg ggcagaaaaa aagtttttag tttattaaag taagaaagaa
     9121 acacttggaa tcattctact ttttcctagt atttctttct tgattttttg tctttttttc
     9181 aaaatttttc gctacgtaaa agaacgaata tttatatgaa atttctatga agttgattga
     9241 attccatgcg cttttttagt atagttaagt ttgtgtaaaa attatagaag aggaagtatt
     9301 ctatgaataa atggaaaaac tggttattgg gatccggact tgtcagtatc ctgctctttt
     9361 tatctggctg tgtgcggatg gattcaaacg gaaaccctga cacttcggga atcgtttatc
     9421 gagtactcgt ccatcctatg ggccaagcga ttacatatct agttgaaaat ttcaattggt
     9481 cttatggctg ggcagttatt gcaatgactg tgatcgtccg tatcatcatc ttgccactag
     9541 gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa aatgcaagcg ttaaaaccac
     9601 aagttgaagc agcccaacaa aaattgaaag ctgcaacaac acgtgaagaa caaatggcag
     9661 cacaagcaga aatgcagcaa gtataccgcg aaaacggatt gagtatgacc ggcggaatcg
     9721 gctgtctgcc tctattgatc caaatgccga tcttctctgc cttgtacttt acggcacgtt
     9781 atacagaagg tatccgggaa tcatctttct acggcatcga tttaggctca ccaagtatgg
     9841 tacttgttgc cattgcaggg gtcgcttatc tgcttcaagg atacatctca acgatcggta
     9901 ttcctgaaga acagaaaaag acgatgcgta cgatgctgat cgtctctcct gctatgatcg
     9961 tcttcatgtc gatcagtgcg ccagccggtg ttacacttta ctgggtagtc ggtggtgtgt
    10021 tcagctgtct gcaaacattc atcacaaacg ttatgatgaa accacgtttg aaagcacaag
    10081 tggcagaaga gttgaaacaa aacccaccaa aacaagtcgt cactccaaga attgatgtga
    10141 cagaatcagc taaaccagct gacagcaaac aagcaaccaa acaaatcaag caacccaaaa
    10201 acagtacaaa caataaaggc cgcaacgcag gaaagcagca gaagcggaag tagatagtaa
    10261 ataagagtgt aataaaaatt cattaacagt tttagcaatt aaaaatatcg accaagattt
    10321 ttgaaaaaga aatctggtcg atatttttat ttaagtgaat gtttaatttg aatcatcgat
    10381 tgatcgctat ctaattgcag cagcatcttt ttatgaagaa acaacttttt cttatctatc
    10441 aacgtagaca ggtgttgctg tttcttctaa tagtgtactt aattccttaa tattttgttt
    10501 tccttcttct aacatccaac tactcgcttt ttcttcgcct tcggtaacaa aaggcgccac
    10561 tcccttcgcc catgttgctc gcccacataa gacaccattc tccataaagg acaacgcgga
    10621 gataccaccc atctaaataa catccataaa cctttctagt agaagaatag tgatagtatc
    10681 catccctttt tctactggga atacaattac acgattcaaa gcttgataca caacatttcc
    10741 cttcttataa accgctattg taaccgaatt gttttgaatc acctttttgg gatagaaact
    10801 ggctcctttc cgcaacttct taaatgttgg tttcttttat actttctttg tagcaactac
    10861 aaatttcttc aaatctctaa atgtttgcat aacaaccgcc tttctgactt ttactcagga
    10921 tggtatttaa aaatcagcaa aataaggctt ctctccatct tttttatttc ctagctgctt
    10981 taaagacact cttgctgttt tttaatctct atcaaggggg ctctgaatct attttgatga
    11041 acttatctta ttaatttctc aaatgagctg tcagacacaa ctgtattttt tcaatctcat
    11101 accggacaac tatgccagct taaaaaaatt tcctctggca aaacttcttt attgaaaaat
    11161 gacaatgaaa aaagaccatt ctttttgttg aatgctcatt gtgtaaacat atttcctgcg
    11221 actgctcttc gaaccattct cagtgcaatc cctaatttat ctattaaatc ttcttttagt
    11281 tcctcatctt tcatattcaa aatttccttt gtatgttaca caagtttgtt ctctaaataa
    11341 cgcgaattgt aaaattaagt tagaaaaata aaaaggcatt tatggtacac tcaaattgta
    11401 tttccgacga aagaaaacaa aggagtgtaa ccataaatga cctacaaaca tcttaccata
    11461 gacgaactga caatgataga atcatattat cttcaacata ataagccggt tgaaatcgct
    11521 aaccgaatgg gacgtgctat acaaactatt tataatgtag tcaataagtt caagcaaggc
    11581 aagactgctc ttgattattg gcaccagtat aaagaaaata agaaaaaatg tggtagaaaa
    11641 gtcattcaat tacctgctca tgaagtagat tacattaaag agaaagtcac tcttggttgg
    11701 acgcctgacg tcattatcgg gcgaaaagaa aggtctgttt catgcggtat gagaacactt
    11761 tatcgtttat tttctaaagg aatatttgat attgacacac taccgatgaa aggtaaaaga
    11821 aaacccaatg gccatcagga aaaacgggga aaacaacagt atcagcgctc aatccatgat
    11881 cgacctgata attatcctga tttcaattct gagtttggtc accttgaagg tgatacgatc
    11941 gttggcattc atcataaaag tgccgtcatt actttagttg aaagattatc taaagtcatt
    12001 atcacgatta aacccaacgg ccgtaaggca ttagatattg aaactgccct taatcaatgg
    12061 ttttctcgct tccctaaaaa cttctttaaa tctattacgt ttgactgtgg aaaagaattt
    12121 tctaactgga aagccattag taaccaacat gatattgata tatattttgc ggaccctgga
    12181 acacc
//
LOCUS       DAZKLA010000083        12126 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000083 DAZKLA010000000
VERSION     DAZKLA010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.084"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            179..373
                     /locus_tag="ACG85N_001957"
     CDS             179..373
                     /locus_tag="ACG85N_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224693.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            370..864
                     /locus_tag="ACG85N_001958"
     CDS             370..864
                     /locus_tag="ACG85N_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224694.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(1026..2198)
                     /locus_tag="ACG85N_001959"
     CDS             complement(1026..2198)
                     /locus_tag="ACG85N_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0224695.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(2404..3219)
                     /locus_tag="ACG85N_001960"
     CDS             complement(2404..3219)
                     /locus_tag="ACG85N_001960"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0224696.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(3369..3815)
                     /locus_tag="ACG85N_001961"
     CDS             complement(3369..3815)
                     /locus_tag="ACG85N_001961"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0224697.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(3812..4327)
                     /locus_tag="ACG85N_001962"
     CDS             complement(3812..4327)
                     /locus_tag="ACG85N_001962"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFQ0224698.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(4400..5314)
                     /locus_tag="ACG85N_001963"
                     /pseudo
     CDS             complement(4400..5314)
                     /locus_tag="ACG85N_001963"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(5311..7638)
                     /locus_tag="ACG85N_001964"
     CDS             complement(5311..7638)
                     /locus_tag="ACG85N_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFQ0224699.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            8247..8393
                     /locus_tag="ACG85N_001965"
                     /pseudo
     CDS             8247..8393
                     /locus_tag="ACG85N_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(8481..8774)
                     /locus_tag="ACG85N_001966"
     CDS             complement(8481..8774)
                     /locus_tag="ACG85N_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFQ0224700.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            8811..8996
                     /locus_tag="ACG85N_001967"
     CDS             8811..8996
                     /locus_tag="ACG85N_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224701.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            complement(9177..10355)
                     /locus_tag="ACG85N_001968"
     CDS             complement(9177..10355)
                     /locus_tag="ACG85N_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0224702.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(10459..10773)
                     /locus_tag="ACG85N_001969"
     CDS             complement(10459..10773)
                     /locus_tag="ACG85N_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0224703.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            10880..11095
                     /locus_tag="ACG85N_001970"
     CDS             10880..11095
                     /locus_tag="ACG85N_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224704.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(11395..11637)
                     /locus_tag="ACG85N_001971"
     CDS             complement(11395..11637)
                     /locus_tag="ACG85N_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0224705.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(11624..12052)
                     /locus_tag="ACG85N_001972"
     CDS             complement(11624..12052)
                     /locus_tag="ACG85N_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0224706.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         3523 a         2368 c         2109 g         4126 t
ORIGIN      
        1 aacagaacta tagcaaaaaa tcccgtgtat caacacggga ttagattaag tttcctcaat
       61 cactattcat aacacaatta ttataacaag taagtaaaca attgaaaagg acatttattg
      121 aaaacggttg ttttttactt aaggaggaaa tttagcgaaa aaattagttg cattgttttt
      181 ggtcgcagga agtttgttga gtgggtgtgg tgctactacg tctactactc aaagcacaag
      241 ttcagatcag gcttccagca gcagtaccac gatctccgaa agtacggcag attcatcgaa
      301 agaacaagaa caaattgaaa agaaaaaaga agagaaacaa aaagcggaaa aagctgcact
      361 agaaaaaaag tgacagaagc ggaatctgct ttgtcacaag ctgaagcaaa tcctacaaaa
      421 gaaaactatg caactgcttc agcagctatc caagcgctac ctgaaaacaa acaagagttg
      481 acaagccgtc ttgctgcaat tgattccaca atcaaagcaa aagaagcaga agaagaacgg
      541 aaaaagcaag aagaaattgc acgacaacaa gcagaacaag cgcaacaaga agcaatcgca
      601 cagcaacaag cagaacaggc gaggcaagaa gcaatcgcac agcaacaagc agaacaggcg
      661 cgacagcaat cagcagctga acaggcctct cagcaggcac aagccgcagc tcctgagcaa
      721 aataatgaac aaactgtcta tgtcacaccg accggttcaa aataccatac acataaatgt
      781 ggaaatggta cgtatacccc tgccacttta agcgaagctc aagctcgtgg tttaacccct
      841 tgctccaaat gttatggaaa ctaattcaaa agttatacta taataataaa attcttctga
      901 aacataaaaa aatagaagat ggcgttccag atagtaaacc aaaaataaca gtttacacgt
      961 ccatttcgtc agcttctctc ttttttcatt tagtctgttc cctctgcttt tttggacatc
     1021 ttcattcatt ctttctcaat caaatagtac acctcttttt cttttcgcag tttatatctt
     1081 ccttttttca atttcattga aaactgtttc gaatgtttat gttttacatg gattttaagc
     1141 aggagctcct catgttgcaa gcgatagttc ctgcctttta aatcagaaaa tctcactagt
     1201 ccgtattcat cagaagcttt cacaaatgga actttttctc tagtaatata gtgtttgccg
     1261 ttgagcgttt ttacagaaaa gaccattcct tcgattggtc gtccgtttgt gttctttaca
     1321 taaaaagata aaggatagtt tcgcttagcg atggccaaag aatctaaata agcgatgagt
     1381 agccaaacta agccgattga tccaagtagt ataccgataa accaaataaa aaatttccat
     1441 tttttccatt gatcaattcc ttggatcgct ttttttgcct cttttgcttg ataaggaata
     1501 cgatgccccg tcacaagcag tcgatgtgta ttgaccatat aaggagtaca ggtaagcaaa
     1561 gtcacgagat ctctgccttt ttggataccc aaagaatcta cttcatcagg taagaccact
     1621 tggatttttt ctacttgata cgccaatgtc tttccattga tctggatgaa aaactggtct
     1681 ccttttttca acttaggcaa atctgtaaat aattttgctt ctgggagtcc acgatgccca
     1741 gaaagaacgg catgggtgtt tttcccgcca gtcggataag aactttcttc caataatgac
     1801 gttccttttt gcaaaaatat ttccgttgtc tgatcgaata taggtaagct gacattgatt
     1861 tttgggattt cgatgactcc aagcatatgt tccataaaaa aggcattact aggtaaatct
     1921 tttaatgctt ttgcatcaac tgcttggttg aaactgccta tccctggtga tacgttttgc
     1981 tctgccaact gttggttttt ctttgtcatt ttttcctgct gtttttttat ttcctctgaa
     2041 ttcttattcg atgcttgctt ttgataatgg atgatcattt gctgggcaag aaaatcattc
     2101 agtgaatctt ctacaaaagg ataagaaaag atcccgatcc caacgactaa aatgattgcc
     2161 atgaagatgt ccagtattct tattctgctt ttattcatca tgtccaccta ttttctaata
     2221 tgtatatgaa gaggttgtga aatcagcgcc ttgacctgag tattagcgaa gcaaacggca
     2281 aaatagttcc tcctattttg ccgtttgttg agcgaatacc aagagaccga ccacttgtca
     2341 caacctttct ccccatttga atcactatcg tttatttatc gtatgatttt tgtttccaat
     2401 agtttaagct tctgtttctt gatggcgttt tttgaaatag atcactgctc ctccaactaa
     2461 agcagtacct accaagatga atacgatgat ccccatacca ccagttgaag gcaagattcc
     2521 tttaggttgg ttatcgatcg ttgtttcatc agaagttgtt ggatcatcat ctactgtaaa
     2581 ttcagtgtac gcagcttcat tcttaggtaa tgcgtagcct tctggtgctt ttgtttcttg
     2641 aaggcagtat gccccttgtt tcagcccttc gattgaaaat ttcccttctg aatcagaaat
     2701 caatttcact tcttcccagt cagaacctgt gacttcatct gaccaagcat atgggtattc
     2761 tcctgaggca aggtttgcta tttttttcat taatttaaca gttggatctt ttgctcttag
     2821 aatagaaaat tctgcatcgg ctaatttttc ttgcgtgttc acatcttgtt tgatgaagtg
     2881 gaaatcaccg tagttttgga cattttctac agtaatcgtg ttatccgtag ttgtattttc
     2941 ccgattgact acaccgtttg gattcccaag attggcttca tctgcgccat ggatgatacc
     3001 ggttgtttgt tgcaattttc cgttttttac tataaaagta gtttcttttt gtggattggt
     3061 taattgataa ccatctggtg cttttgtttc aactaaagta taattgccgt ctattaatcc
     3121 attgacactg atggatcctt cagtatcagt tgtgaatgtc aacgctgact cagcaggatc
     3181 atagagagcg acaccagtgt tttcatcaaa accggtcacg aattgattct ctgaattttt
     3241 gatctggaat tgcgcccctg ctaacttatc tacccctgaa aagttcccag ctttctggat
     3301 tttgatattg ctttctttga tgacattttt agcatatagg tgaatcgtgt ctaaaggttt
     3361 gtcagggtct agggtaagct taacacgata tttggcgacg tttcgacacc gggctttggt
     3421 gtttctacaa agacgtatac agcatctttc ccatcagatt tacctggaag agtcaggtta
     3481 gctaatcctt caccagaagt cgttacactt gcgaagggtg tagcgtctgc tggaactgct
     3541 gtgtctttac ttggatagta cgtagcagtg atgtcatata cgttgaattc aacgccgttc
     3601 aacggttcgc tattcggaaa tgtcaattcc gttccgttgt ttatttgatt ttctggtgct
     3661 gaagtgaatt tcaatttatg caaattaacc gtaacatttt ctggtgtagg tgatacggct
     3721 gcagaaacct ctgcagttcc taggccgaga acgaaaggaa gcagcatgat agcagttcct
     3781 aaaaacgtcc atatttttgt tttgatgttt ttcattttat ttccctccta ttgtttcctt
     3841 tttgttgttt tttataaaac caagctgtcc ctgttgaaag gactagcaat aaccctaaaa
     3901 ttgagaaacc tccaagcatc tcttcgcttg tttttggaaa gcttcctcca tttttttcat
     3961 ttgatggacc atttgtctgc atctgaactg gcgtttttcc attattcgtc ggatttgctt
     4021 ttggttcgtt gatgatttcc aatggtatag gatgaagcgg atcataactg cctttttcaa
     4081 cttcaaagag aatggttgat tgactcaata tatatccttt aggcggttta atctccacta
     4141 gttcgtaact tccaaaagac aatccatgga cagaaaatgt tccatcttta ctggaagtaa
     4201 aaatcgttgc tcttgaatca tctttggcta cccaatgatt tatcccgttt tcttgcgcta
     4261 agtattggtc attttttttg actagaaatt gagcatctgc tagtttttgt ttcgtttgcc
     4321 gatccacttt gacaaattgt ttgcctcctg tttggatggc aagatgtgtt tctactgttt
     4381 gattgtcagt cgtcaaacgt acatcgttat caaatgtcgt tttttcactg ttttctttca
     4441 atgtcatttg gtaacggatc gttaattttt ttccagcaaa cgaactaaga tcttttccag
     4501 aatctgataa gacaaaacca tgttttattt tttgtgtgtg aaatgtatga atcttgtctc
     4561 caccaatcaa aaaatcgatc gtttctggtt ctagccataa ctgaggatca gctgtatctt
     4621 tgatcgtata ggttcctatc tcgttgatgt tctcaggaat tgttacggtg atgagataag
     4681 ggactttatc cccatctgca aaatctgtct gcttattctc tactgttttt gttaatgtga
     4741 gtgcagcagg tattttttgt tcgctctttg gatacaagtg gatggtcgtc aatttatgat
     4801 tagatgaatc tgtcacagga agcacaacga caaatggtgc tgatttttct tttacttaat
     4861 ctgaagtctt tatttccgta aatcggtaaa cagcgtcacg accttgctca tctttgtccg
     4921 atgcactaaa agaagcaatg ccctcttcct cgtctactgt ttttgtcaca ttttctgcca
     4981 gtattttttc actatcactt ttttgtgcta gttttctttg tgcgtcttcc acactacttc
     5041 cttctgaccg cagcttgtaa aattcatccg agacatcata tatagcgaaa gtaacgccgt
     5101 tcaacccttt tactccttga aagttatccg ggatatttat tccgtcgttt tgactgatac
     5161 ttggcatctt atcgctttcg aacattcgat tatgcaaagt aaatgtaata ggtgattctc
     5221 tagctgacac agtcggagaa cctataaaaa agaccagtag gataaaaaag agaacagcaa
     5281 atatatccac acgtacattt tttgtttttt tcatcgctgt tcacctgctt ttttcttacg
     5341 atggataaaa taaaagaagc cagaaaaaat aaagatcagt cctaaaaaga caaagggcag
     5401 tagacctatc ccacctgtcg aaggaagcaa gagagtcgga ttgatcgtca acttagcggt
     5461 atctgttgcg ataatcgtat aattattgtt tacacttcct tcgcctaaac taatcgttac
     5521 tatttgtgat ccaatatctg tccggctgtt aactgttagg gtataatcca atgattcgcc
     5581 taagacaaca ccggtaacgt ggagatttcc aaccgaaatc gagtgacttt gggcatcata
     5641 attaaaggca gcgtcatcga cagtaatcac tgcgttagct ttatagatgg tataagttcc
     5701 tgatgtgttg gctatatcag taaatgacaa cttgggattt gctgctttga ctttgtctat
     5761 tccgctattc gttagtttaa ttgtatagct gccagcatta accatatttt ttgctgattg
     5821 tgcatctgct gtgtaatcca tactgctaag tgccacttgt actggagtaa gcgaactagt
     5881 gacaccctct gctagcttga catggattga cgggacacct gcttttccat catatgtctt
     5941 actgccactg agctctaccg caccgctgat ttgcttaatc gtatagacat cagatgtctg
     6001 cacgatagta aataaatagt ttgttctggc agctagttta gctagtccgc tttttgtcaa
     6061 ttgccaagac gtataggttc caatttcagt aggcgcagtt acttcaactc ccttttcatt
     6121 catcgctttg aaatcagttg gattccatgt gatttgatca atactgagat cattgtttaa
     6181 aagggatgtt gattctgttt tatcattaaa ggcatattta acgatcggta caacagaaag
     6241 accatcatag tacttactgc catttgcatt gattgatact gttgcatttg tcttataata
     6301 taggatgact tcagaaatct gttttccatt tgcgtccttt acgattacat ttccatttgt
     6361 atctaaacta gctaaaacac tcattgctga ttggttcttt gtttctatcg ctgaaacagc
     6421 tgtaaaggta tatccgttga tagtaggcgt attagtcgtc aggtcttgtg cagcacttcc
     6481 gtaagtaaga ttcattattt tagaaggagc aatcatctgt ccaaaatagt cttgatactt
     6541 aatagtgatt ggtgtttgtt tgcctgtata ggtcaccgta tcgacgttac tttctagggc
     6601 tgtgaaagaa ccgtttgttt gtgcaaaaga atagcccgtt actgctgttg gagtgataac
     6661 cactttgtca cccacatgga ttccagataa tgtagtcccg tacgttccga tttttgctgc
     6721 tccaccattg actgtataag aaaaagttcc tgtccgattc gcattgacct gaaccgttgc
     6781 tgcttgatag gttggaaaat ttgaaacatt tcccttacca tcaacgatct gcccattttt
     6841 gatcgagtac cccgtgaatg gaatatcatt tgctaactgt ataccgattg aaaaagtact
     6901 catacctcct ccggcaggaa gccctcccca tgttgagggt gtaaaagaat aacttttatc
     6961 cgcattgggt gtaactgaat cgatcgtatt tccagcggta gccaccccca tatagatact
     7021 ttttgggaat gtgatagtag taccatttac tgtggagcca ataaacagat tatggatatc
     7081 tggacggtta tcaataaaag cgattgcttt ggttacgcct tgtaacgcag ttttatccgc
     7141 actcatcgat ccgttaaccg caacaatgac tccatctttg gcatatatca ctgtatatcc
     7201 atctggtatg gtatgtccgt tgaaattcgc agtggccgca acagtactaa atgtctggct
     7261 tctacctgtt tctgaataag ctttctcccc tgctgctttt gctgctgtga tttctttttc
     7321 tgtcggatca atcagttgag tcgaggatgt ggttgaggtt gtctcgttgt tgctagactg
     7381 attggtgctt tctatggact gtatttcaga atagggtgga ctatttgtct cctctgtcgt
     7441 ttggttagca aaatttgacg tttctgtata aacgtttaca taaatacttg ataagctccc
     7501 taagaaagta aaaatcaaga tgatcgaaat cataatggga tgtaagtatt ttcttttaga
     7561 tacagctttg agtctagaaa caaaatgttt ttgacatata cgttggattt tattttgagt
     7621 tttcttaatc attttcatcc ctcctctcaa taaaaaatca tcaaataatc ctttacacgt
     7681 tattctaaat ttaattttta ttattttatt gatgtataga aagtttgggg ctttttttct
     7741 ctttttattc taaggttatt ttagtcttgt cctttaatac aaagatttta tttttaaata
     7801 aaaaatactc tttttttacg attattgaaa aaaaaagata aaacttgatt aattgaagta
     7861 ttgcttaaat ttggaagcta tgagggaaga ataaaagagt taggacggga ttatctacta
     7921 tttttctttg ttttaaatat ttgttatctt tgaacataaa agagaaatgt gtcgactgca
     7981 gtgttgtgtc cctcatcacg aataaacggt gttcaactag ctgatcttgt attcacttaa
     8041 aaaatggtaa agtacgagtt gctattcccc attttcaatt ttctaacgct caggacaaaa
     8101 tactgttttc acaacctcat tggaataaaa gcatatatat tgtttcaaga acgaaatctt
     8161 gttatctttt taaagggcat ctgtccaaaa tctaatttta ggaggtacgt tttggtattt
     8221 attcgtacaa ataaaagaga attatcttta ttcatgtaac gcatccaatt gattttttca
     8281 tgtacgctcc tggaacgatt ttactgacga ttgcttattt aactgcgaat cgctcattag
     8341 cttttatcat agctatccat attttgaata acgttcttgg ctttgtatta tagatacaga
     8401 tgacaaaaaa agttcaagta tgcggtgtag aaccaatatc gcatacttga acttttttca
     8461 atgagtataa attaaaactt ctaattttca attcccatca tctgatatgc ccgaccgtct
     8521 gaacttcttt tgaaaaagcc aaatgtgatt agataacgtc tttgttcgac ataatcgcca
     8581 ctagaaaata cgttcagcag tatcgaatta acctctgttt ctgtataagt cactccatgg
     8641 tcaaagcgtt tcaagacttc tttcaatagg atgtatttct tttttgttga acttggtact
     8701 tgttttactt ttccatcatt catgaatgtg tctaaaactt tcatcttttc ttgctccgta
     8761 taagtataaa ccatcttctt tctccttttg attctaagaa tactccattt atgttagaat
     8821 attctggatt ttttattaaa gcggttactc ttaagaaaga tattatcata gtgctctttt
     8881 ttttactact acttataggt agatttattc taatccatta ttctccccta ccaataatta
     8941 aagaaaaaac tggatcaggg tgttcaaatt acatgctaag aaaaaagaac ccttgaatac
     9001 caagggttct ttctcagtct gataacaatg attaacgacg gatttctttg atacttgtga
     9061 aaagtcgtta aagtattgat ataaaaggat ttttattacc tctgactaca tattgactaa
     9121 tttgttaaaa aatcgttttt caagaatcct gtaataccag tgctttcacc gtttttttaa
     9181 ctctctaaag aactaaaagc aatcagaggt tttacctttt catttgacgt taagtggcga
     9241 taaattttcg aagttgtttg gtcagtatgg cctaagcgat ctttcaagac ttcccagcta
     9301 cagtgttccg ctactaagct tgccattgta tgacgagcta aatgagtatg aaatggctta
     9361 ttaattcctg agcgattcac gcaccgtttg acgtgatcat ttacatactc tcgacgataa
     9421 ggaacaccac gtttactaga atcaagatac acaaacaaat atgtttcttc tataataaac
     9481 ctgtaatgtt gcctcatctc acccaaagcc ttaatcaact gttgtagctt ttccataact
     9541 agaggagtta ctagaactcg tctagcagaa ctaactgtct tagtagtttc aattcgaaaa
     9601 tccttactct tacctttaac tgccgtttgc ttatagaaca gaatttcatt tcttttgaaa
     9661 tctatatctc caggctgtag agcaagcgcc tcgctgactc ggcagccagt gtaaaataga
     9721 acatggaaaa gtacagacat cggtaattcc tcatgaacaa tgccataatt caaaaaagtt
     9781 ctcaattcat cgacagtaag aaacttgtcc tcaatattat ttatggcttc ttttaaatca
     9841 gttaccgtta actgatattt tggcattctc acagacttca ttggattttc acttatcaaa
     9901 ttctttcttt gacaatattt aaaaatcata tttagaactt gaataagtga ttgtaaacta
     9961 tttttggcat agccaacatt tgttccatta acgccacatt cctttaaggt aaaaagcaat
    10021 tcttgaaggc actctggtgt caaatcagac aactttttat caccaatcca tcgaacaata
    10081 atttttaatt gttcctctcg cttatcatag gttatttcct tttgttcatt taacgaacgg
    10141 taatattgga accattgctc acctacaaca ctaaacttgt cattgcttgc aacaattttt
    10201 ttcttaagcc tttgttgttt ctccatttcc agataagcga gttgcgcctc acgttttgaa
    10261 ttaaagccac gtcttgttgt tcgatatgct tgaaagtacc atgtgatcct accagtagaa
    10321 ctgatttgct tcttgaatcg atctgttttt tccatttatt gattcctcct attttgaagg
    10381 aacccaacaa gcagaaaccc tttggaaatc tttctatcta tgtcatcatt gtaccgcatc
    10441 ttagaaagaa aatgtaactt ataaagatcg aagtagtcgc aatttttctc gatagtcatt
    10501 cgaatcactc tttctcatcc attctaaaac atcatcacga aaatagtgtc ctcgaatccc
    10561 gtaccaacag ggaggaaact caccaagttt tactaaacgg ttcagtgtac tatcacttat
    10621 ctgaaatagt cgctttaaat cagtatttgt taataccaga ggtaaatgac tgactgcaag
    10681 caatttttcc gtgtaatctt ccagtttttt ttgtaaaatt gaatagaact ctatttccag
    10741 agaattaaca tttgttttaa cttccaactc catcaccctt ctctctcaaa atcaaaacga
    10801 ccaccacggt accccttaaa attgtaaaaa aacactccag tactccatca aaacggacta
    10861 gaaaagagga atttttaata tgaaacaagg aattgcagac ataaaaataa ttaaagaaat
    10921 tcttgaaaaa tctacggcta atgcaatagc attcggaaca ggaatcaact tgagtacagt
    10981 aaaaaaactt aaaagtggag agagagctga agaaaaatta aatcttgccg atgctattaa
    11041 aatcacagaa tttggcatga aaaatatgcc aaccaaaatc gaaatatgga aatagtagtt
    11101 tttctgatct atcagctgtt ttgccaaagc acataggaat ctcacctccc cccagtaatg
    11161 gcgggccgat ttggttacgg aagtatcatt atcgtagtcg cgattctcat attaaaattt
    11221 gtcgcttaaa agctaacctt gtcatggcgt aactagagaa tcaaaatcta catgtactga
    11281 tagatttgct attcatttgt caaagagcgc taggctattt tcagcctatg gaaatccttt
    11341 attttgaccc gataaaaaac ttccatagaa tgaaaaggaa agggaaaaaa tactttaacg
    11401 agttgtatca aattcaagta tcttctcaat caattttgtt tccagttgtc tacgcatata
    11461 ttcatctaca tacataacca ttcctttttc ttcttcataa actggtttca tggctaaccg
    11521 tgaaatataa ttttgatatt ttgacagaac gatggacatg gcaattgaat caccagaagc
    11581 tgcggctttg attgtcgtta gtggtatgaa tggtatatat cgtttatttt gcttcatatt
    11641 tgacacccaa atactttcct agctcttcca aagccatttg tcgttggtac caaacactgc
    11701 cgagactttt cccaataata tcgccaattt cacgatcatt aagaccgatg aagaaggaca
    11761 taaaaatgat ttcttgtctt tgactaggta atgctgcaat cgcatttgcc aactcctcat
    11821 tggaaataaa gattttcata ccaaggacaa taaaagtacc actactattc aaatttggtt
    11881 ccaaataata gaactctgta cctaaccctt tttgttccaa tatttccatt gatatttgcc
    11941 gttggcgaaa tctagcgtac ttcttttgaa tatttaacgc ctcattgcag actactttct
    12001 tacaaaaact gtcaaacgta tgttgaatgt gggtttcctc tgagtcgccc ataatattct
    12061 ccccctcaaa aaattcattc aactcgtttt acttctatcc ctttccatta ttactacaat
    12121 ctagga
//
LOCUS       DAZKLA010000084        12086 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000084 DAZKLA010000000
VERSION     DAZKLA010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.085"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..460
                     /locus_tag="ACG85N_001973"
     CDS             140..460
                     /locus_tag="ACG85N_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224707.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="ACG85N_001974"
     CDS             912..1112
                     /locus_tag="ACG85N_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0224708.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="ACG85N_001975"
     CDS             1259..4048
                     /locus_tag="ACG85N_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFQ0224709.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="ACG85N_001976"
     CDS             4095..4622
                     /locus_tag="ACG85N_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFQ0224710.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="ACG85N_001977"
     CDS             4643..5833
                     /locus_tag="ACG85N_001977"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0224711.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="ACG85N_001978"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="ACG85N_001978"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFQ0224712.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7966..8640
                     /locus_tag="ACG85N_001979"
     CDS             7966..8640
                     /locus_tag="ACG85N_001979"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0224713.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8637..8978
                     /locus_tag="ACG85N_001980"
     CDS             8637..8978
                     /locus_tag="ACG85N_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224714.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9008..9367
                     /locus_tag="ACG85N_001981"
     CDS             9008..9367
                     /locus_tag="ACG85N_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFQ0224715.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9627..9749
                     /locus_tag="ACG85N_001982"
     CDS             9627..9749
                     /locus_tag="ACG85N_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224716.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            9891..11975
                     /locus_tag="ACG85N_001983"
     CDS             9891..11975
                     /locus_tag="ACG85N_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0224717.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
BASE COUNT         4032 a         2110 c         2601 g         3343 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaatggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactt caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ccatcatcat
     5341 caatggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtggggaa
     5401 ttctgagatc atcaatggaa tgattgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggtgag caagatacag tagaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatatccg cttgtggcag aactgccagg
     5581 tgtttctctt gaaaagccgc aaggagcttt ctatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctgaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcac atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatac gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttacgatga
     6721 tgcaatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtaat
     6901 ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacggactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggttgtg cgcctgccgt tcagctccga
     7201 gtcacaagga gtaatttcaa aaaacagttt ttcatgtttg atgactattg tgacttgtgg
     7261 ccggagagca acgtttattt aaaaaaaaga actgcgaact ctcgcagttc ttttttttaa
     7321 atacgggata atggaagttt ccagcagaca agagaagaaa gcaaaagaaa ttgctatttg
     7381 attcacaagg ttctataacg aaatcgcaat caatcaaaca tttacaaaga aggtaattca
     7441 aatggaaatc tgatttgatg atgcttctgc tgttgaaata aagcagcatc tagcacctga
     7501 aaacaaacta atagtaacat tcgaagatgg agtaggtccg tattttcagc atgcaataat
     7561 ccatatgtaa gttcaattca cccttgaaat catcggacca gataaagtta tgaccaatct
     7621 atcccaagca atctagaaga gatattagta aagggacatt tactagaagt cttagatgag
     7681 cctataaata tatgtttcga tggcgtcatc agtggtctga ttgacggaag cttgggtgtg
     7741 atcgatctct ctggaccaaa caacgaagga atcaagggga atccggcacg ttaagaaaaa
     7801 aatagcataa aaaactagac acttgatgag cgttcattca attgtttgaa ggaactaggc
     7861 tggtgtcatt ttttatacat atattttctc tttcgtcttg atagggctta tgggataata
     7921 taaggaagaa aatcatcgtt ttattcaaaa aggagttttt gacaaatgaa aaaacgtata
     7981 ttatttatcg gtgatagcat tacagacgca ggaagatacc gagcagaagc aactgatcta
     8041 ggtaaaggtt atccactttt gattgccgga aaactaatct cccaatatcc aacgatggaa
     8101 tttgaatgca tgaaccgtgg gataggagga gacaaaattg cagatctgca agtacgctgg
     8161 caagaagatt gtttagcttt acaacctgac tatgtatcga ttttgatcgg aatcaatgat
     8221 acctggcata atactagcga tcaggctata ttcggaactg agaaatctgc tcagcaattt
     8281 gaatccgttt atcgaaaatt actaaatgaa cttcaaacag cggaaatctg caacatcatc
     8341 ttgatggagc cgtttgtact tcctataacc gacgaccgtc atggctggcg taaagactta
     8401 gaccctaaaa tccaaattgt tcgtaaactt gctaaggaat atggcgcatt gctggttcct
     8461 ttagatggta tattcaatgc tttagggatt gcttatggtt tttcgaaata tacaagagaa
     8521 gatggggtcc acccaacgat tggcggacat gagatcattg cacaaagctg gatagatgca
     8581 attgtcgcag tcgatttttt tagtggagat aaaaatgagc gaaagaaaag tgaaaaatga
     8641 tggaaataag gtgggcagta aaagaagatt tacctcaaat caatcagctg attactgatc
     8701 atccagaatc attcgtaaaa gcaaaaacgg cagctgacct aaatgataag aataaactat
     8761 tgctcgttgc tgtaacaaaa gaaaaacgga ttatcggatt tgcgcaagcg gacgtgcaac
     8821 agtcagaaga acgattggat cagcgaccat caggaaaact aatccaacac acattagtaa
     8881 caaaagaaaa tacggaagaa atccaaaaaa aatttgcaga atacttgatg ctggaagcta
     8941 aaaaaagaag aataaaatca atttttgccc agttttaatc aaaaacagta atagaaggag
     9001 aaatacaatg agttgtttag gaaatgtcat ctggtttata ttcggcggac taatcggtgg
     9061 tcttagctgg ttagcagcgg ggatactttg gtgtatcacg atcataggta tcccaatcgg
     9121 tatgcaatgt tttaagatcg cctccctttc ttttgcgcct tttggcaaag aagtaattta
     9181 tcaccagtct ggcatgtcat tgatcgttaa tatcatctgg ctgatcgttt caggcctacc
     9241 aattgctctc gcacatgtcg tcagcggcat ccttctatgt atcacgatca tagggatacc
     9301 ttttgccaag caatctttca aacttgctcg attggcgttg atgccattcg gtgcagaaat
     9361 tgtataaaag agaataataa aggttagata aagcatagag ccaggtggga tacatgtatt
     9421 tcacctgact ctatgcgttt tcctgtataa gttcttctat gtgactttta cgtcttttat
     9481 ttatatgggg ttactgttta tggaaagttg aaataatttt cattaaagaa ctctttctat
     9541 atttttgaaa gcgtagtgga gcttcgtata tctgggaagg acattttgca gggattgaaa
     9601 aaagatattg tcctatcgaa aaattaatga atttagacaa attgccagca acaggcttca
     9661 aacttagttg ctatcctgtc aagatcaaga aggcaagtgc gggttggata agagcagtgg
     9721 caatgatcga agagaaaaag aaagaataag ccaaaacatc tgtatggaag aaattttccg
     9781 atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac cattcattta
     9841 taattagtta tgaagagata ttcatatgtt gaggcgagga ggtcattaaa atgaaaagtt
     9901 acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa ggagttcaga
     9961 aaatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta acgattgacg
    10021 cagaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg aaggaattag
    10081 aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat ggaaatgaaa
    10141 aaaagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct ttattgctat
    10201 tatttgattt taatgaacca attcgcttga tagggtattt attgatttat ctgctgatag
    10261 gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc tttgatgaga
    10321 attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat ccagaagccg
    10381 tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca gtcagtcatt
    10441 caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac gtgcagacgg
    10501 cggaaggatt gatgaccgtg aaccctgaag acgtgctgat cggtcagttt gtacttgtga
    10561 agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca ttagtcgata
    10621 cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa acagtattaa
    10681 gcggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct tatgaaaatt
    10741 ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa gcaccagctg
    10801 aaaatttcat tactaaattc gcgcgctatt acacaccagt cgttgtagga ttggccgttt
    10861 tattagctgt tttacctcca cttgttgttt ctagagcggc gttttctgaa tggatctatc
    10921 gagcattgac atttttagtg atctcatgtc catgtgcatt agtgatttcc gtgccactta
    10981 gtttctttgg cggtatcgga ggagcaagta aaatcggtgt attagtaaaa ggttcaaatt
    11041 acttggagtt gcttgcacaa actgagacag tggtattcga taaaactggc acattaacga
    11101 aaggtgactt ttcgatccag acgatcgatc caacaggaat tgatccaaag aaatttatgc
    11161 aatatgttgc aagtgcagaa cagttttcta cacatccgat tgctcaatct gttttagaag
    11221 gctacgcagg tccgctttta ccaacagcta atatccagga atttgcaggt gaagggatct
    11281 tagctgaagt tgatggcaaa caagtattag taggtaatca caaattgatg gaaagatttg
    11341 aaatctcttt tccttcaagt caagaaatcg gtacattgct ttatctagcg atcgatcaaa
    11401 gttacagtgg ttatcttgtt attgcagata ccttgaaaga agatgccgta gacgcattgg
    11461 ttcaattaaa acaggctggt gtgaaaaaca cagtcatgtt gacgggcgac tcgaaaaaaa
    11521 ttgctgatca tatcgggaag caagtaggcg tggacaagat atatagcgaa ttgcttccag
    11581 aagataaagt gcagcggtta gaggaaatct tacaaagtaa caataaaaag accgcatttg
    11641 tcggagatgg aatcaacgat gcaccggtgc tagcaagagc agatgtagga atcgcaatgg
    11701 gtggattggg aagcgatgct gcaattgaag cagcagatgt agtcatcatg aatgatcaac
    11761 catcgaagat tgcagaagca atccatttag caaagaaaac attgaaaatc gtcaaacaga
    11821 acattgtctt tgctattggg ataaaaattt tggtactttc cttaggtgct tttggttttg
    11881 catcaatggg agatgcagta tttgcagatg ttggggtgac agtattagct gttttgaatg
    11941 ccatgcgaag cttgcatgta aaaaataaac gttgatcaat gaagaggacg aatgaacgtc
    12001 ctcttttttt tatatccttt tcttaatgaa aaatgtaaag aaaatagata ggattataaa
    12061 ataactaatg tttttaattt tgcaga
//
LOCUS       DAZKLA010000085        12085 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000085 DAZKLA010000000
VERSION     DAZKLA010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.086"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(135..722)
                     /locus_tag="ACG85N_001984"
     CDS             complement(135..722)
                     /locus_tag="ACG85N_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224718.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            897..2219
                     /locus_tag="ACG85N_001985"
     CDS             897..2219
                     /locus_tag="ACG85N_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0224719.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2374..3150
                     /locus_tag="ACG85N_001986"
     CDS             2374..3150
                     /locus_tag="ACG85N_001986"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0224720.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3143..3712
                     /gene="rnmV"
                     /locus_tag="ACG85N_001987"
     CDS             3143..3712
                     /gene="rnmV"
                     /locus_tag="ACG85N_001987"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFQ0224721.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            3705..4589
                     /gene="rsmA"
                     /locus_tag="ACG85N_001988"
     CDS             3705..4589
                     /gene="rsmA"
                     /locus_tag="ACG85N_001988"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFQ0224722.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5040..5669
                     /locus_tag="ACG85N_001989"
     CDS             5040..5669
                     /locus_tag="ACG85N_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224723.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(5793..6902)
                     /locus_tag="ACG85N_001990"
     CDS             complement(5793..6902)
                     /locus_tag="ACG85N_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224724.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7111..7533
                     /gene="mgsA"
                     /locus_tag="ACG85N_001991"
     CDS             7111..7533
                     /gene="mgsA"
                     /locus_tag="ACG85N_001991"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFQ0224725.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      7720..7818
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            7856..8392
                     /locus_tag="ACG85N_001992"
     CDS             7856..8392
                     /locus_tag="ACG85N_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0224726.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(8432..10195)
                     /locus_tag="ACG85N_001993"
     CDS             complement(8432..10195)
                     /locus_tag="ACG85N_001993"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224727.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(10198..11913)
                     /locus_tag="ACG85N_001994"
     CDS             complement(10198..11913)
                     /locus_tag="ACG85N_001994"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224728.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLASNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         3774 a         2225 c         2463 g         3623 t
ORIGIN      
        1 tgtgttaatt ataagttcta tcactttatg ttcaattagt ttgtgctctt atatgtgtga
       61 ttgaaaatat agataaaaaa acaatcatcg gctttgactt cgatgattgt tttctttatt
      121 ttgtaaaaat cctatcactt gattttatgg taacctttta tttttgtctt caaacggctt
      181 ggtaagagag cagtaagcag tgcaaatggg atcgaaaaga cataccagaa aatcgtacgt
      241 ccgataccat aatgttgata gagctttgtc aacatcacgt gtaacaagat caaccctaaa
      301 agcaaggagc cagctataaa ccaatgatta tcccaatgca tgacttcact aagtgccatc
      361 actccaaaag gatagaaata aaaatagatc ccgactaagg tcatccgcca gttctttgta
      421 aatgttccga tattcaataa aaagaaaatc gctgaaagca aaataccgat aggcggaaaa
      481 agcccaacca aaaaggaata caacatcccc cacaagatca tcacaggacc tttgatcaat
      541 gcaacgattg cgatgaacaa agtcagcaat attagttttt gtgataacgt tgcaacagtc
      601 attaatccaa ataaaattaa tgtgaaccaa tgccaatggg actgctgttg ttctttacgg
      661 gaaaaatcga cccggttgga taaataatct gctaatttcg gataagaacg cttaaaatcc
      721 ataaactgtt cctccttcgt ggaaaaggta ggtttagtat acgaaaaata aaaaagaaat
      781 acatccgtct taagagggag tttttcactg aaagtggaga aaaaagaatg gtaaattaaa
      841 atattctcag ttatactgaa aattatacca aatttcaaag aagaaagagg atgggaatga
      901 aaggcaaact aaaacgagaa atcaatctgt ttggcgcatt agctaccgtt atgggaaccg
      961 tgatcggtgc aggcgtcttc ttcaaaactg ctgctgttac tgctagtact cagtctacaa
     1021 gtttgacatt gctagcttgg cttttgggtg gtttcttaac gatctgtgct ggattgacag
     1081 tggctgaatt agctacagca atcccggaaa ctggtggagc tgtaaaatat attgaagctg
     1141 cgtatggaaa attgcccagc tttttattag gatgggcgca aagcttgatt tactttcctg
     1201 ctaatattgc tgcattatcg attatctttg cgacacaatt gacgaactta ttacaattat
     1261 ctaccgatta tcttttgttg attgcagtga tcaccgctgt ttctgtgaca gggttgaatt
     1321 tgttagggac aaaagtcggt gcttctgtac aatcagtgac gttgattgtt aaattaatac
     1381 caattgctgt gattgtcata tggggattat tgaccccagg acaaggaact gtccagttat
     1441 ttccaatcga agctggaaaa gatgttactt ttgtcgaagg attgagcagt gcgttgttag
     1501 caacgttgtt tgcttatgac ggctggttag gtgtcggtgc aatggcaggg gaaatgaaac
     1561 ggccggaaaa agatttgcct aaagcaatta ttcttggttt gagttttgta acagtcgttt
     1621 atctattgat caattttgtt tttttgaaaa ctttgccgat tgatcatctt gccggtaatt
     1681 tgaatgcagc ttctgaagca tcagacgtca tttttggagg aatcggtgga aagctggtca
     1741 ctattgggat cttgatctcg gtatacggtg cattgaacgg ttatacacta actgggatcc
     1801 gcgtgcctta tgcgatggct ttagaagacg agctaccttt cagtaaacag ctgacaaagc
     1861 tttcaaagaa gtttacggtt ccttatgttc ctgcggtttt ccagttagca gtagcttgta
     1921 ttatgatgag ccttggttct ttcgactttt taacagacat gttgattttt gtgatgtggc
     1981 tattcagttt actgattttt atcggtgtct ttattttaag aaaaaaagcg ccggaattgc
     2041 cacgtccata taaagtacca ctttatccaa tcgttccgat tattgcgatt ctcggtgcta
     2101 ttttcatttt aggtatgacg atgatgacgc aaacgaaatt agcattgatc ggtataggtg
     2161 ttaccttgat aggtatccca gtctattatc aaaaaaagaa aaaacgctca gaagagtaaa
     2221 atatttcaac aacagacaga aaaagtacga actaggtcct ctagatcttc gtgctttttc
     2281 tatttcaata aaacaagttc ttctattttg atttttgcta cttctgcaag tagtgcttat
     2341 gtgctaaagt aagaatgaaa aggagagata aacatgatat ttgattctca tacccattta
     2401 aatgctgaac agttcaatga agatattcca gaaacgattc agcgagcaca agaaatgggg
     2461 gtaacgaaaa tggcggtcgt gggcttcgat acgccaacga ttgaaaagtc tttgcaatta
     2521 agccatgact atccaaatat atacagtatt atcggctggc atccgacaga ggctggcagt
     2581 tatacgaaag aaatcgaaaa taaattacaa gaacaattga ctttaccaaa ggttgtagca
     2641 ttaggggaaa ttggcttgga ttatcattgg atggaagacc cgaaagaagt ccaagataaa
     2701 gtatttcgca gacagattgc tatcgcaaaa gaaatgaacc taccaatcag tattcatact
     2761 agagaagcac ttgaagacac gtatcaaatc ctaaaagaag aagatgttcg agatatcggt
     2821 ggaatcatgc atagtttcag tggtgattat gaatgggcaa aacgatttct tgacttaggg
     2881 atgcatattt catttagcgg tgtcgtcact ttcaaaaaag cattagacgt ccaagaggct
     2941 gcagcaaatg taccaatcga acgattactt gtggaaacag atgcccctta tctggcacca
     3001 gttcctttta gaggaaagcg aaatgaacca ggctatactc gttatacagt agaaaagatt
     3061 gctgaacttc ggaatcttcc gacagaagaa gtggctgccc aaacatggac aaatgcgcac
     3121 cgattatttg gtctaagcga acatggataa gatgaagatc caagagatta tagtcgttga
     3181 aggaaaagac gatacaagaa ggctgcggga agtagttgat gcagatacga tagaaacaat
     3241 agggtctgca atcaatgaag agatcctgat gcaaattgaa catgcccaag agactcgtgg
     3301 agtgatcgtt tttaccgatc ctgatttttc aggagaaaaa ataagaaaaa tcattatgga
     3361 agctgttcct caggcaaaac atgctttttt gccgcgcaca caagctaaac caaagaaaaa
     3421 gggaagcctt ggtgtcgagc atgcaagtga tgctgctatt ttagaagcat tgaaaaaggt
     3481 cgtgacgcca gacatttccc aaacggctgt ttctgaaatc actagacaag accttttaga
     3541 ctatggttta atagctgggg cacaagcaaa agaaaaacga gaaaaactag gtgacgagtt
     3601 gcgcatcggt tacacgaacg gcaaacaatt ggaaaaacgt ttgaagatgt ttcgtattac
     3661 gccgaaagaa ctcgcccaag caatgaaaat agtggaggaa caacatgagt gaatatcgtg
     3721 aaattgcgac accctctaga acaaaagaga ttttattgaa acatggtttt tcatttaaaa
     3781 aaagtttagg acaaaatttt ctgactgaac caaatatttt aaggaaaatc gtagaaactg
     3841 cgcaaataga tgaacatacg aacgtgatcg aagttggacc aggaatcggt gcattgacgg
     3901 aacaattagc caagcatgca aaacaagtcg tagcatttga gatcgatgat cgtttgatcc
     3961 ctgtattggc tgatacgatg caaccctatg acaatgtgac tattgtacat caagatattt
     4021 taaagaccga tttgtcaaca gctgttagag aaacatttca tgaggagctt ccgttaaaag
     4081 tcgtagcgaa tttaccttac tatattacga caccgatcat gatgcatttt cttgaatcag
     4141 atttggttgt ggatgaacta gtcgtcatga tgcaaaaaga agtagctgat cgtatctctg
     4201 ctgaaccagg aacgaaagct tatggttcat tatcgattgc tgttcaatat tatatggaag
     4261 ccagtttagc attcatcgta ccaaaaacag tatttgtccc tcagccaaat gtagattcag
     4321 cgattttgaa actgacaaga agagatatac cggcagtcga agtgacagac gaaaaagcat
     4381 ttttccgatt gacaaaagcg gcatttcagc aaagaagaaa aacgctttgg aataacttgc
     4441 agcattcgta tggcaaagat gaccagacaa aagcatggtt agcaaaaagc ttggaaacag
     4501 ctggaatcga tccaaaacgt cgtggggaga ctttgtcatt acaagaattc gccgcattaa
     4561 gcaatgcgat gtcagaaaat atgcagtaaa aaaagaggtt gtgacagaag ttgtgaaaaa
     4621 gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat gtttttcgtt
     4681 tgtttgggct tattaccgca gatgcaagct tttgaacacc gtttaagaaa gaggttgtgg
     4741 gcctgccgtt taactctaag tcacaaggaa caatttcaaa aaacagcttc tcatgttttt
     4801 gagttagttg gtcttattac ttgatgcaag cttttgacca ctattatgcc gaacaaggag
     4861 atgtgacaca aattttgtca catctcctat ttttgtttct tgatcaaaaa attcttatac
     4921 ttgatactca cactcatttt gtatggattt agaaaagaat atgtgtcttg acagttatta
     4981 gtaagtgagt tatactaagt cgacatttat gtgtcaagac atattaggag agagaaaaaa
     5041 tgagaaaaaa ttcagttcga aaactaacga tttctgcttt attgattggg atgggggtca
     5101 ttatcccaat ggtcatgcct aaaatcatga ttccaccagc ttcctttact ttggcgtctc
     5161 atgtgccatt atttattgcg atgtttttta caccaggagt tgcagtggct gttgcgctag
     5221 gtacgacatt tggtttcttt ttaacaacgc cggttatcat cgcattgcga gcattgtcac
     5281 atcttgtgtt tgcgttgatt ggggcttggt acttgcaaaa gcatccgagt atcgttttaa
     5341 aaaatggaca gtttactttt gctaattggc gtttccaagg gttcaatttt gtgatcggtg
     5401 tgattcattc tttagcagaa atgctagtag tcagcatctt ttactttatt ggtaatatgc
     5461 ctgaaacata ttattctcaa ggatattttt atacagtatt catcttgatg gggattggtg
     5521 ggcttatcca cagtttagtt gattataata ttgcttattt tatagcagga acactcagca
     5581 aatattttga tatccccgta tttacggcag caaaaaaaga aaatcggaaa gagtcgattc
     5641 aagggtacgc gaagaaaacg acatattaga catgatctct caaaaaaaaa actctcatct
     5701 agtaatggat aataaatcct ctaagatgag agttttttgt cctaaaaaga gctgcaccaa
     5761 catcttctgg cacagctcta acagggggca atttatggaa gtttgatgac ttgttctgtt
     5821 tctttatcaa agaagtggcc tttgctgatg ttgaaggcaa ggtcgactat ttcgcctggt
     5881 ttatggaaat ctcttgcatc tacttttgag atgaactcag tagaaccagt ttttgtgtaa
     5941 agcatcgttt cagctccaag taattcggaa acgacaactt ctgatttcac tacggcttca
     6001 ggtgaagctt caagggctac ttgttcactg tggatgtctt ctggacgaat accgaagata
     6061 acttcttttc cttcataacc tttttcaact aatactttgt tttttccttc aggaatacga
     6121 agcttaagcc catgtccatc tgtgataaca ccatttttca atgtcacatt aaagaagttc
     6181 atggcaggag atccaataaa gccggcaacg aacatattga cgggtgtatc ataaacttct
     6241 ttcggtgtac cgatttgttg gataaatccg tctttcatga taacgattcg gtcagccata
     6301 gtcattgctt ctgtctgatc gtgagtaaca tagatggtcg ttgtttcaag acgctggtga
     6361 agtttggcaa tctcagcacg catggctaca cgtaatttag catctaaatt tgataatggc
     6421 tcatccatta agaagacttt tgcatcccga acgattgccc gtcccaatgc cacacgttgg
     6481 cgttgtccac cagataaagc agctggtttt cgtttcaagt attctgtcag acctaagatc
     6541 tctgctgcat tgtccacacg ttttttgatg tctgctttat catatttacg taatttcaag
     6601 ccaaaagcca tattatcaaa aacagtcata tgaggataca atgcgtagtt ttggaaaacc
     6661 atggcaatat cacggtcttt aggtgccacg tcattcatga cttttccgcc gatatttagt
     6721 tctccttcag agatatcttc cagtcccgcg atcattcgca aagtagttga ctttccacaa
     6781 ccggaaggac caacaaaaac gataaattca cggtctttga ttgatagatt aaaatcagtt
     6841 acagaataat tttctgcatt gtcatatttt ttatatacgt ttttcagagc catttctacc
     6901 ataatacgtt ccacactttc ttttttagtt ttattaacta tttaaagtat aaatgaaagc
     6961 gttaactaac ttcaatgtca tgttgaacaa gaattttttc ttttttttgt caatgtgcta
     7021 gttttaagct ttaataaata tccataagaa ataaaaactg atacactgga gtaaataagt
     7081 atgttaacaa tatcaaaagg agtgtctctt atgaaaatcg ctttgatcgc tcatgaccga
     7141 aaaaaaccta tgatggtgaa attagcaaca gcgtataaaa caatattgga aaaacatgaa
     7201 ctttttgcta ccgggacgac tgggcagagg atcacggaag caaccggctt gccgatccat
     7261 cggtttaaat ccggaccgtt aggcggggat cagcaaattg gagccatgat ttcggaggat
     7321 cagttagatt tggtgatttt tcttcgagac ccgctggctg cacagcccca tgaaccagat
     7381 gtaacagcac ttatccgttt atctgatgtc tatgagattc cgttagctac gaatatcgga
     7441 acagctgagg tgctactaag aggactggaa gccggcttcc tagattttcg aagaatcgtc
     7501 catgaaatag acaagcatcc actttcattt tgatggataa gcgtatggaa aaataacgtt
     7561 taatgttcgc ctatatgtag ctataaaagc taaacaaact gataatatgc gatataatac
     7621 gaacgtattg gataatataa acaataatat tagtttttcg tatttgcagt aaatgtgaaa
     7681 atatgctata gtttctgtgg gaaaagatcc caaaaataat acagagtaga aaataaagcg
     7741 ttaagtgcta agaggatggg atgttgcctc ttggacgaag gacgtaatgt tcgcggtttt
     7801 attttcgcat tcgctgcatg atacgatgta gcagctctta aaggagatgc taaaaatgaa
     7861 gaaacaccgg ttagacgcac gtatgattgc tattatgggc ttattgattg cattgatggt
     7921 cactttgtcc cgactggtag caatcgagac cccatttatt aaaatcagtg tcacatttat
     7981 tccacaagtg atcatgggca ttctttttgg tcctttttgg tcagggatag gcgcagtttt
     8041 agctgattta gtaggaatgg ccctcttctc gaaatctgca ttctttattg gatttacatt
     8101 gaatgcattt attgaaggag caatctacgg attctttttc tatcgaaaag agatcacttg
     8161 gaaaaatgcc attctggcta cgcttagtgt cacgctgatc atcaacttat ttttgacacc
     8221 attatggctt gcgttgatgt accatgtgcc attgttcagc tgggtcgttt gggcgccccg
     8281 cctgttaaaa acagtaatat ggttaccgat tcaatcaatt gccatttatt acgtaggacg
     8341 tagtattcct tacaaaaaaa ttttaagaag tttagcgatc cacgctaaat aaaaaacaag
     8401 cagtcttccc atcccagaag actgcttgtt tttattgtgc tgtgaattgt gcttgataga
     8461 gccgtcggta ataaccatct ttttgaagca gctcatcatg ggtgcccatt tcgacaatag
     8521 ctccctgatc catcactaat atctggtccg catttttgat tgtggacagg cgatgggcaa
     8581 tcacaaaact ggtttttcct tccatcattg ctaagaaggc ttgttggatt tttttctctg
     8641 tcaatgtatc gactgagctg gtagcttcat ctaagatcaa cataggggga ttactgatca
     8701 tcgttcgagc aatcgtcaac agttggcgtt gtccatctga tatctttaac ccttgaccgc
     8761 caatcttagt atctaatttt tccggcaaac gttctacaaa ttcatacata taagaggctt
     8821 ttagggcatc attgatttct gcgtcacttg cttccggatt cccaaaacga agattttctc
     8881 gcaatgtact gtcaaatagc caagtatctt gtaacaccat tccaaaactt ttacgtaatg
     8941 tatcacgttg gatagagcgg atgtcatggt catcgactgt aattgttcct tttgttgtgt
     9001 cataaaaacg catcagcaaa ttaacaagtg tggattttcc ggcccctgtt tttcctacta
     9061 tagcaatggt ttgacctgga taagcagtaa aattgaaatc ttcgatcaaa cgctgcttgg
     9121 ggtcataaga aaaagaaaca tgatcaaaag agacttttcc tttgatattt ttgggttcca
     9181 aggcataggc atctggcaaa tcaggctttt cttctggctg atcgatcaaa tcaaatgctc
     9241 gttctagacc agaaaaagcg gtttgcagtt gtgtcgtgat tccagaaagt tcgataaatg
     9301 gtttagaaaa ctgacttgag tagatcgtaa agctggaaat cacaccaaca gtgattgacg
     9361 aaccactctt caatgccaat aggccgccaa ctaaaccaat cgataaataa gcaaggtgat
     9421 ctacaaaacg agaagacggg tttgtcaagg aagaagaaaa ttgtgcccgt tgtccttttt
     9481 catatagatc agcatttagc agttcaaatt gctgttgact agcttcttct tgctgaaaag
     9541 ctttcacgat tttctggtta ccgattcgtt cggtcacaaa gcccgagata tccccaataa
     9601 tcctttgctg tctatcgaaa tttgtttgtg aagcacgagc aacgatccaa ctcacaagga
     9661 aaataattgg tgtagaacaa agaacgacca atgtcagaag tggacttaga cgcatcataa
     9721 aaataaacga aagaatgatg attgccactc cagaaaataa ttgattgaag gccgcagaaa
     9781 ttgcaatgga aatattgtcc atatcatttg taaatcgact gacgatattt ccgtgggagt
     9841 tttgatcata ataacgaagt ggcaatcgat tcagatgagc aaatgcatct tttcttaatt
     9901 ttgcaacgga taagtaagct aaccgattgc ttaacacttg gatgagccat tgactgacta
     9961 cgttgattaa aacgatacca ccgaatagaa taagtaaatg gaacagtcga ttgaagcgga
    10021 cctgtccttc tccaatcatt tgatcgatac tttgaccaat ttgtaacgtc atcacaactg
    10081 ttgcaatccc attgatgatt cctagtatca cagctagcca aatgtctttt gcataggcag
    10141 taaggtaagg aatgaatcgg cggagcgtct gcaaggataa tttttttact ttcataacta
    10201 ttcttcctcc tgtgatgcga cgatttcctg atattctgct gattgagcaa gtaattcttc
    10261 atgggtaccc tgagcagcaa tcttaccttg atccatcact aagatatgat gtgcttcttg
    10321 aatagagcgg acacgttggg agataataat cactgtactg ccaagataag tctgtagcgc
    10381 cttacgcaaa tccaagtctg tttgataatc caacgcactt aaagagtcat ctaaaatcag
    10441 taatgctggc ttggcaatca aagctcttgc aatcgtcagt cgctgttttt gtccgccaga
    10501 gaaatttttc ccattttcca tgacttttgt atctaaacca tttggaagct gacggacaaa
    10561 ttcttctgcc tgagcgattt tcagagcttc ccaacattct ttttctgtgg cgtctttctt
    10621 accccattga aggttttcac gaatagtccc ggtaaatagt acagaagttt ggggaacaag
    10681 agcaatttgc tgacggagat gatccagctg ccaagaacga acatccattc cgtccattat
    10741 aactgtaccg gaactgacat catagaaacg cggaatcaat tgtgtcaaag tcgttttccc
    10801 gcttccggtt ggaccaatga ttccaaggac tgtatttcgc ttcagctgaa atgtaatatt
    10861 ttggagagaa agaccagaat caggagtata acggaagtca acatggtcaa aagcgataat
    10921 tgcttcagaa gaaaaatcgg gagtagtact gacctcagct tcaagaatcg aattttctgt
    10981 gtctagcacc tctttcaccc gatttcctga agcttcggca cgtgtgaaga tcacgacgag
    11041 gtttgaaaca acgatcagtg ccaaaagcat ttgattcata taattgatca aagcaagaac
    11101 ttgtccttgt tgcaagctac caatatttac tttgatcccg ccaactgtca gcaaacagat
    11161 gatcccgaca ttcatgatca aggttgtagc aggtgataat agggcagaga tattggaaac
    11221 acgtaaataa ttgctcgcta gatcatttgt gcttttatcg aacttctctg tttctttttt
    11281 tgtctgcgca aatgcgcgga tgacacgaac accgctaaga ttctgactaa cgtgttcatt
    11341 gagctgatct agtttttctt gcactttttg gtagagagga acggtttttt tgatgataaa
    11401 aaataaaatg attgagaaaa taggcaataa tgctaagaaa aacagaccta cttcccagtc
    11461 gatcacaaaa gccatgacca ctgacccgat acttaaaaac ggtgctctga tcaataaacg
    11521 aatgaccatt gctaaagcta gctgaagctg attgatgtcg tttgtcattc gggtgatcaa
    11581 ggtatctgtt ccaaaatgat tcaactcgtt gtgagagaaa gtattgattt ttttgattag
    11641 ctgattacgt aactctgtac caaatccttg tgaagctact gaagcatagt attggcaaat
    11701 gatagcacaa atcagaccaa tcacagacat agcgaccatc cataagccca tttcaatgat
    11761 ttttcctcgg tcattcattt ttagtccatt atcgatcaag gaagccatca ttaaaggcag
    11821 gacaagttcg aagacagctt caagaaattt gaagaacggg cccaaaatga tttgcttacg
    11881 atagtctttt gcataacgta ataactgaat catatgattc ctcctattat ttatttgtcc
    11941 acaattgaaa aaacaagtac taccacattg tagcttagga aatttagaat gaaaagagaa
    12001 caagcctttt cataaatgaa agttttatta tgaagaagct ttagaaattg aatagactat
    12061 tttttttaaa aaaaagaaaa tgaga
//
LOCUS       DAZKLA010000086        11975 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000086 DAZKLA010000000
VERSION     DAZKLA010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.087"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..406
                     /locus_tag="ACG85N_001995"
     CDS             <1..406
                     /locus_tag="ACG85N_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFQ0224729.1"
                     /translation="KVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISK
                     NSEDEDKIKKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDA
                     FIKSAAQFIAADYDADLADQFINGNNFMPTTN"
     gene            complement(552..1028)
                     /locus_tag="ACG85N_001996"
     CDS             complement(552..1028)
                     /locus_tag="ACG85N_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0224730.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(988..1344)
                     /locus_tag="ACG85N_001997"
     CDS             complement(988..1344)
                     /locus_tag="ACG85N_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224731.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(1322..1507)
                     /locus_tag="ACG85N_001998"
     CDS             complement(1322..1507)
                     /locus_tag="ACG85N_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224732.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(1508..1780)
                     /locus_tag="ACG85N_001999"
     CDS             complement(1508..1780)
                     /locus_tag="ACG85N_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFQ0224733.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(1777..1977)
                     /locus_tag="ACG85N_002000"
     CDS             complement(1777..1977)
                     /locus_tag="ACG85N_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224734.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            2105..2317
                     /locus_tag="ACG85N_002001"
     CDS             2105..2317
                     /locus_tag="ACG85N_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224735.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(2350..2544)
                     /locus_tag="ACG85N_002002"
     CDS             complement(2350..2544)
                     /locus_tag="ACG85N_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224736.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(2557..2739)
                     /locus_tag="ACG85N_002003"
     CDS             complement(2557..2739)
                     /locus_tag="ACG85N_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224737.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(2736..2876)
                     /locus_tag="ACG85N_002004"
     CDS             complement(2736..2876)
                     /locus_tag="ACG85N_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224738.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(3366..3731)
                     /locus_tag="ACG85N_002005"
     CDS             complement(3366..3731)
                     /locus_tag="ACG85N_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224739.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(3771..4073)
                     /locus_tag="ACG85N_002006"
     CDS             complement(3771..4073)
                     /locus_tag="ACG85N_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFQ0224740.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(4073..4372)
                     /locus_tag="ACG85N_002007"
     CDS             complement(4073..4372)
                     /locus_tag="ACG85N_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224741.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(4365..4529)
                     /locus_tag="ACG85N_002008"
     CDS             complement(4365..4529)
                     /locus_tag="ACG85N_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224742.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(4535..4804)
                     /locus_tag="ACG85N_002009"
     CDS             complement(4535..4804)
                     /locus_tag="ACG85N_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224743.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(4816..5565)
                     /locus_tag="ACG85N_002010"
     CDS             complement(4816..5565)
                     /locus_tag="ACG85N_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0224744.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(5562..6254)
                     /locus_tag="ACG85N_002011"
     CDS             complement(5562..6254)
                     /locus_tag="ACG85N_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFQ0224745.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(6260..6931)
                     /locus_tag="ACG85N_002012"
     CDS             complement(6260..6931)
                     /locus_tag="ACG85N_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFQ0224746.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(6924..7265)
                     /locus_tag="ACG85N_002013"
     CDS             complement(6924..7265)
                     /locus_tag="ACG85N_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224747.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(7443..8141)
                     /locus_tag="ACG85N_002014"
     CDS             complement(7443..8141)
                     /locus_tag="ACG85N_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFQ0224748.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            8228..8698
                     /locus_tag="ACG85N_002015"
     CDS             8228..8698
                     /locus_tag="ACG85N_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224749.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(8741..8920)
                     /locus_tag="ACG85N_002016"
     CDS             complement(8741..8920)
                     /locus_tag="ACG85N_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224750.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(8907..9245)
                     /locus_tag="ACG85N_002017"
     CDS             complement(8907..9245)
                     /locus_tag="ACG85N_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFQ0224751.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(9261..9464)
                     /locus_tag="ACG85N_002018"
     CDS             complement(9261..9464)
                     /locus_tag="ACG85N_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0224752.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(9479..10255)
                     /locus_tag="ACG85N_002019"
     CDS             complement(9479..10255)
                     /locus_tag="ACG85N_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFQ0224753.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            10554..10874
                     /locus_tag="ACG85N_002020"
     CDS             10554..10874
                     /locus_tag="ACG85N_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0224754.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            10892..11323
                     /locus_tag="ACG85N_002021"
     CDS             10892..11323
                     /locus_tag="ACG85N_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0224755.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            11453..11914
                     /locus_tag="ACG85N_002022"
     CDS             11453..11914
                     /locus_tag="ACG85N_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFQ0224756.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
BASE COUNT         3497 a         2307 c         1833 g         4338 t
ORIGIN      
        1 aaaagtttct cctacatttg ttgatattta tactcaagcc acacaagcta aatctgagca
       61 attggaacaa attgctggag ttggttatag aaaagctcta gaatttctaa ttaaagatta
      121 tgcaatatct aaaaattcag aggatgaaga taaaataaaa aaaatgttat taggtaaaac
      181 aattgaaaat tatttgaatg attttcctaa actccaaaga ttagccaaag ctgctacttg
      241 gattggtaat gatgaaacgc actatgttcg tagacatact aataaagatg ttcatgatat
      301 ggatgcattt attaaatcag cagcgcaatt tatcgcggct gattatgatg ctgatttagc
      361 tgatcaattt ataaatggaa ataactttat gccaactact aattaaaaga ataaaagagc
      421 cacagatacc aattaataaa acggttggga tatcttggct ctttcatatt tttttgacac
      481 taatagaata tcatgctgct aacaatgttt acatagctac aatgaccgaa catttagtga
      541 acagtgtaat tttagaataa agagagttgt tctttgtact tctcgctatt gtaaaaatca
      601 atttcgtttt cttttttcaa tcggtcagct tgtctgtgtt catactcatc taaaaagttc
      661 aatgtctttc taatttctgc atgtcgctgt ctgacatacg atgagctata cccaacagat
      721 tcagcaatat cttctaagct catttgatca acatatttca tcttaacaat ttgattatcg
      781 ataccagaaa agctatctat cagcaataac atttcttctt tttgctcgat caacaattcc
      841 aattcatttt ctatcttttg gatattctct tctaaagaag aagttctcga attcttttcg
      901 atgcggacgt ttgctaaatc accacagacc catcgattca attcaagctt acttttatta
      961 agattccact ttaagtaaag aatctgctca tctagttctt ggtaatcttt taaccaatga
     1021 aatctcacaa acgccacccc ttatggtaga atagtattgc gctgctatct cgaaaggggt
     1081 agctttttta ttttttctta gccgttccat tctggattgc cttttgttcc aaacgtcgtt
     1141 ttttcttctt aatctttgat tttgtttttc ccatacaatt ctcctttccg aaaacacatt
     1201 ttgttcgatt tttaatacta aataaaagtt ggccagaatt tttcgttcgt ctttactcat
     1261 ctttttttga ataagattac tagcataaaa atagttagta ccacgaaaat gaacaggtaa
     1321 atcaattacg ttcaccttct tcatgccagt tatcagccac tagaatcttt gcgttagtgc
     1381 cgccaccgtt ttcttttttg tatttttcaa atgctttata agcagctgtt tttgatttat
     1441 agatttttcc ggaaccgact gggaacatcc atgtgccctc tgtgatagct aagatgtata
     1501 ctttcattta attcacccct ttaatcccca acactacata tccggacttc tgcatgtaat
     1561 cagtaatata agtgatctct gctttgtatg agtcaccagt gtaactaaat gttggtctcg
     1621 ttagtccagg taattcatgt ttaatgtatt ttttatattc acgtagtatt aactgatcac
     1681 caactttgaa gtcacgatca ttcttgcgga tctcgaattg tttgcgtccg ctcgtcactg
     1741 cttcaaaata ttctggtagt atttttagtt cgtggatcat tctgtttcct ccttcaaaat
     1801 tcgctcatca tcaaaaagtg aatctccaca aaatccttct cccaaatatt tatcgttcat
     1861 gtataagtca accaaatatt tgtgatctgc ttctcgatat tcattaatat taacgatctt
     1921 tattttgtaa acattacctt cgtcgctcac tatcttgaat tgttctctga ctttcaccac
     1981 cgttcctcct ctttttgaaa aaattttagt gtttttgtaa gaccccctaa atcagctttt
     2041 ttatttgata taatacttgt atcgattaat caaatcgaaa ttaaatttaa aaaggtgatt
     2101 tatcatgaat atcaacaatt tagtaaaact cgttgaacaa cgttttaaag aatcaggatt
     2161 agaacaatta aaaaaagata attctccaga attattttct aattttaccg aagacgacat
     2221 tcctactctt aaacaattaa aatattattc tgattattgg acgcaaactc gtgagaatga
     2281 attaaattta gaaaaactca ttattaaaaa gaaatagcaa ctccagccca cacggctggt
     2341 tttttagttt caacttccct ccttacgggt caaaatcatc ggttccggca taatctgatc
     2401 atcctttagc aacaaatcag gatatttttt ccttagcttc ttattgttcg tgtaagtttt
     2461 caatagccat tggttggctt cgcttttgct ttctccagaa aaataaattt tccctgtttg
     2521 gatgcctgca agttgaatca tcattttcac ctcaaatcaa attttttctg ctagatggag
     2581 caaaagcaat ccgacatcaa gaatcttttg acccattatt tcagctactt tgctcggctt
     2641 aactccttga gaaaagagca agcgtgcttg ctctatctct tcgtgagtcc aaataaattt
     2701 ggcttcctca aggataatta ctggatcagt tgctctcatt gttcctctgg aaaaatgatg
     2761 tcattcttat gcttggacca ggtatctgca tatggaacaa agtattgtct agctaattcg
     2821 atttgatcaa ttaaagcagt ttctgataat tcatggtctg cagcaatttc attcatacta
     2881 tcgccttcat ctaaacagat aagaccgttg acgttatcga tttttacatt tgtgactgct
     2941 tgactttctc cacgctcata atattctaca gttgatactg atacatcagc ttcggagttt
     3001 aattgcagaa tctgttactg ccgggataat ttaatattct tttcataatt cacctttttt
     3061 cctactgaca tcgaatattt ttcatgttat gattagccgt gaaaggagag ctatattatg
     3121 tgtttacaca tgatgtcctt agctcattcg gttctttgga agcttttttg gaaataactc
     3181 tcaggttctt gacgcaaatc tcatttattc tttgaaacaa aaaacttcca ttttccgaga
     3241 gctttggatc acttcaaaag agatttgcat ttatatttct gtgaccttga gtcgcgtaaa
     3301 gaaccttaca gcaatggcgg caattccact ttcaggaaac cgcctttttt cattgatatt
     3361 ggtggttagc ggactaccca ttttctttcg gcagtgccaa accacaaatg atctgtgttg
     3421 atgtttaggt agttgtatac cagataagct aaatccaaag tgcctgcatc ttcaagacga
     3481 caatattcca aaaattctcc catagctgga cataaaaatc ctggagctat catatcccaa
     3541 cagttaatag tgcaaagaaa ttcgtttagc ttctgaacgt tttctttggt ttcacgaatt
     3601 acaacatact ccaattcttc tccgctggca gagcattcca aaacttcaaa ttgattaaga
     3661 aatggtagaa catatgcttc aaacatcaac attccatctt tctgtttttg cacttgtttt
     3721 gcatcaatca tgtctatcct cctattcagt tatttcttcc attaatggaa ttatttcagg
     3781 tcactggact taacgaaaac accattgacc atctttccag tccgaccttt gatttcgtca
     3841 taagcaaaat ccaagcactc gtataaatcc atatcatttt gcattgccaa gatgatcaag
     3901 gttactacta catcgccaat tccgtctctt aaaccatgtt catcttttct agctagagaa
     3961 gcggcaactt ccccgatttc ttcgaccgtt tttaacattt gcttgctgaa atccgcttga
     4021 tccaatccct tatcttttgc ccactgctct actttcgcga ttagttcgtc cattatttct
     4081 cctccacata cctaaactgt cttccttttg aatcaatcca taagctccta gctctatccc
     4141 aaataatatt tttgctcaga ccagtaattt cagataactg tttagcggta cccgttacta
     4201 gaattcgatc accatgccag attgcaatct ttctcggcgt tctccgtttg ggcttttcag
     4261 tccacattga tttaccgagc ttttggactt ctgcaactat ttttttatct tcctgccaat
     4321 cttctgaata agtcaattcg ataattcgct gcattgccgc tttcttatcc atcccgacat
     4381 tctcctttca gtaatttgag tacttgatca agtgcgctct cacgtccgcc atgaaatgtg
     4441 ttgagccact tgtcttcgta cgacacactt tgtcttaaag cttcttgatg cattaattcg
     4501 atctgtgctg taaatgtctt taggtccatc ttgtttacac ctgctcaagt tcattaagat
     4561 gtttttgcaa gcctttaaca caatcaatga atagtaattt ggtatatgct aaatttctta
     4621 attgcgttcc atcgatatag agtgcgaaat agtatctgag tttactccaa ctcgaacgat
     4681 cattcttaat tccttcaatt ccagcttctt cgagttgatc atatacgtct ctcaggacgt
     4741 ctaattcctc gcccttttta taattcgcta tttcattaat tagttctaga taatcgatct
     4801 tcatttttcc acctcttaga atggtgcttt tgattgtcta ttagctcgtt ctagcgcttt
     4861 tttcttgaga taggcttctt ggtcgattgc ccattcagga agcttctctc gtcttcctgt
     4921 gcgcttgtat ccactgcttg cgttcttagg ttcacttttt tctttccttg cccaacttcg
     4981 aatagttgcc aaatagtttt tataagtctt accagatgat tcacaatact ctgacagtcg
     5041 ttcgattcgc tcttggtaat cattagggaa ttctgttttg agtttctcca tctgctcatc
     5101 tgacaaaaga acatttttat actctccgta tttatgacgg acgggcttag ccttcgattt
     5161 tttcgaaggc ggtaactctc ttatatattc ttttgtatta ttaaatgtat tattaataga
     5221 tgtattatta tctttgactt tttcgtcaat aggggtattg cgtttttcgt caatagggta
     5281 ttgattaatt cgtaggtacc tattgattat ttgattggta ccctctttgt aaatgatttc
     5341 ccgattcaag tatccaaact taatcaaatc acttacccat cgcgatatgg tctctttatt
     5401 cacaccatat aaatctgcaa agtactcatt gcctgcccaa caaaagcctc tttcattaca
     5461 caaggccgtt atctctccgt ataacaactt agtatttggt ttaagtcttt tgtcgtacct
     5521 tacgttggct ggtataatcg cataataact tcgatgttct gtcatttttc ccctccaata
     5581 tttaactttt tgattgtttt ctggtttaat ttgatccctt tgatttgata tttatttttg
     5641 aaattaatca cacctatttt gtgcttctct gtgtgatgga ttctgcagag tgctgcaaat
     5701 gtgtactctg catgatcaac ttctttgcgc tttcgtcttc ctagcgcttt gtcaaagtga
     5761 tcgatgtcag ctcctgtttt gccacagatg cagcagactc tttttgtgat gcatttgtag
     5821 aagtaatatt cttgattcgc aggtaaaatc tcatagcctt ctttgaaagg aatatgatgt
     5881 tcaaagatga aatctaagat gatatttgct aagacattgg catcactcac agttgtattc
     5941 gattcgtctt tcaggcttat tttgcgccct gtgacacctt caaaacgaaa gtagaagaat
     6001 tccttccaga agtccgttgg catgcctgta tcgataaaaa tatcgcctat cagcgcatag
     6061 atgaagtttc gttgctgtac ggtgaaacgt ctaggatcaa taaaacgaat ttcaatgact
     6121 cgatcaccat cgtagccgtc atacatcgtc tttagtcgat caatgttcac ttcctcattg
     6181 atggttgcgc ttatgtcttt tcctttgaac tttttcagaa ccgctgaata tgaatcgatt
     6241 aatggtttaa acactcatat cacttcttat ctaattcttt tctcttagct gctattgctc
     6301 gctccatcaa ggcacattgc tcatagctta actgttcaat agtttcaacg ttatcagcta
     6361 agagccctaa tttatctgtc tgctcattaa catattcgat taaggttttg gtcatatctt
     6421 tacccatctg ctcattgaaa gcttctagaa tcgtctctag catgtttaat ttctttgtat
     6481 cgattctagg tggtgttgga atatcttccc cttgaaatac atataatccc agtccgtgta
     6541 gagccaatgc tttcacaaag catcgcttca atgagttatt gatttgcatt gcatttggtt
     6601 taacaactgg ttggtttcga taatctaaaa caggaaataa ttcggtttcc gtgtgtcctt
     6661 taaccgttac tgagacagat acataagtcc cagtttcatc cataagaaaa ggtttatatt
     6721 cctcaacaag aaagtcttga tgagttccag aaacaaccct gtagtgtttg tactcattaa
     6781 tagttaccgt tgcctgtgga tcattctttt tcataatctc ccacgcgtga gcccaagata
     6841 aataatcaaa atttcctttt ttcttgagaa ttttatttaa cttacgacta aaaagttttt
     6901 caaagttcgt tgtccctttg atttcactca tcaaattctg cctccatttc agcaatgtat
     6961 ttcttacctg gtccgtaata agagatatca atcaagttat ctctgtcgta ctcttctagc
     7021 gcatcaatca agccatcttc gatgacgtaa atatattcag gtttattcga atgcttcgat
     7081 agatggataa gataaacatg atcccaaata gttacatggt tgcctaaatc atcttgatcc
     7141 caagctagtt cttcattcgt caaaagattt cgtctgattt ttcgaccact tgtttcctca
     7201 attttcggct tgccccaatc aggatcaatc aaatattgat ctagagtgga aagttctttt
     7261 tccatatgct aaaatctcct tatgatgtgt tttctttgtg actctttgct tgccggcgga
     7321 gtcacttttt tatttgttgc catgcttttt gcttgtcaat atgttgttgg cttaggatga
     7381 ttggtttata gtatttccac cagcaattag caattgccgt ccctattctt agcgcttcag
     7441 ctctattcat tgtcatcacc aaaaagtctc tgttgtctgt tcagttgatc aatttccata
     7501 cggatcgcag tttctggcaa ccacatttca ataaatgaaa cagcatcatc gaatctctta
     7561 cgaggtaact cgccatatct tgggattgaa aaggtacgtt taaattcaga ccaaaatttt
     7621 gagaatactt ttttgctgat ttcttcataa gctcggcttt cttttccccc tagaactccc
     7681 ataactttca tatttccttt ttgcttaatt tcaaactctt gttgtccgct aattcgcata
     7741 gtatctttaa gcatggaaac atctttttta acatctttca tttcttctaa ttgatagatc
     7801 atcatatctt caattgtttg aggaacagta ttcttgcgaa taacatcttc catttcgttg
     7861 aatgcttcaa tgtatttttg tttgaagtag atagctttct ttcctgtaaa ccccatagcc
     7921 agcaagaaaa atccatctct actaatgaag aaaactcgtc gatttctgcc atatgaatct
     7981 ggttcattac cttctacaaa catctgtcca aaattggaca catctttttt tagtgcatca
     8041 atatctctta aaacatgttg atgttttttc tcgaagcttt ctgccacttg taagctcgta
     8101 gtcacagctt ctttattttt caaaattact aattcttgca ttatttcttc tctccttttt
     8161 ggtataattt agttaaaaac tggtggtgtt tattttgaat ttttttcact taacaatttt
     8221 tcaatggatg tcaataataa gttttatatt gagtacgttt ttaacaggga taaaaataaa
     8281 aggaatgcgc cctcaactag aagttgaact taatgcttct tacttcgctg cagatatgat
     8341 ttatacaaaa gtaatcattt ctaactattc aacagaacca gctatgttag ttaatttaga
     8401 actttcatcc gctaacttaa accgcagatg gtcagctact ccatttaaga aattaattgc
     8461 taaaggagga tcagtcgatg ataatagaat atactctgaa gctgtaccgc taaatattcc
     8521 tcctaaaagt gctatatctt gttaccttgc atttgaagta gggaaaatta attttaataa
     8581 actccttaat caccaaacaa aaatgatatt tactctaaat agaacccaaa tccacaaaat
     8641 tgttgatatc aaaaatacaa acttcccaat agagaaatta gtgaaagaat taaactgagt
     8701 aatagtaata tacattctag tttctctttt ctattcattt ttagtcagcc ccctcggttg
     8761 gctttttcgc tctgtactca gcttcagctt catcaagccc cataaaaatc caaaccatgt
     8821 aaacaatcgt tcctatcaac gcttgcctgc ttccccaaag tcctaaagcg tagatgatta
     8881 gcggtgcgct gaacactagc gctctattaa attttcccat ccgcttgcct ccttaagatt
     8941 tccgaaaaat ttgtttctaa aaattcaagt gttttacttc ttaaaaatag atatgtgtct
     9001 cttccttcaa ctggataata gacaaatcca tttttgtttt tctcgatatc gataatattt
     9061 ctatatcttg ggttctttaa aactctagaa gtaaaccaat catattttct gttaatccgt
     9121 tctagcactt ctggcaacgt catccatcta ccagtatcat cagctttttt taactcctca
     9181 taatccactt gggagataat tacatagcct tctggaattg ggatttttgc ttctagatat
     9241 tgcattagct gttcctccct ttacaattcg tacatagtga taatcgaatc tataattctg
     9301 tttgcttctg cagaagtctt tttaccgttt aaaattaaag ataagtagct tttgctaatt
     9361 ccaaatcttt cagcaagcat ggtgtaagtt aagaactttg aactttcgac atattctttg
     9421 attttttctc tatctcgttg agtgatttct gcaatgtcag tcatactaaa actcctttct
     9481 aaccaatttc ctctaaatcc atttgagggt aatatccttc tttttttagt aattcgtaga
     9541 taaatagacg ccctttctgt gtccatttgg tattcattac aattttagtg ccaccatcgg
     9601 ctttcgggat ctcagttgta tgagattttg tgtatccttg gttcatgtgt tttttgcaca
     9661 ataaccattg gttaccgact tttttctgaa tacctagttt atgaagtaat ttattcatct
     9721 gttgtggaga catcccataa tctgctgcaa tctgactaat tgttactgaa tctgtagaag
     9781 ataatatgct atctaaatag gagattttag gttcgtactc tgcaatcttt tgttctgcga
     9841 ttaatcttcc agttctttct tcttttagtt gagttgctaa ttgaatgatt gtatctggat
     9901 taagcaaagc ttcttctact ttttctggag ttagataacc tccatgtttt ctaattgctg
     9961 gcaacacttc acttgttacc caacgtttaa attttttggc agagggaagt tttgatttta
    10021 agattaaact gtacaaacca gattcgttga tgattgtcat ctctcttgat tgacctgagg
    10081 tcgtgattcg cgaccccatc ttatcttcca aatctacatg ccgcgacaaa gcatctttag
    10141 tgtttgaata acccaaaaca ctcgcaacgt ctttgcctac aaaatatggt tcatcattta
    10201 ctaaaattgt ccgaacttcg ttttgttcga aattaaaaat ttgcggtgtg ttcatatttc
    10261 tcattccttt cttttatatt cgtaaacaaa tttaacaact tttttctaaa ttcaattgac
    10321 agtatctgag taatactcta tactaagagc ataattaaat aagacataaa aacattgatt
    10381 ttaaagcttt cttggcggtc tgcatttaca tatcaatagt gttttttgtt gtctttttag
    10441 ttgttaaatc tgtttacaag atatagtata gctcattact cagaattagt caaattattt
    10501 ctgcgtattt ttctaaactt tttttgtaaa catttagaaa ggttgtttta tcgatgaata
    10561 cctatgaaat aataaaagac ttggcgaaga ggaaaaagat atctatacgg cagttagaaa
    10621 ttaattttgg atattctaac ggttatctag caaagtggaa aaataatact cctaattcag
    10681 atgaattaag tcggattgct gactatttcg atgtctctgt agactacctt ctaggtagag
    10741 aagaaagaga aacccctaaa catgtggatt tatcagaaga cgatactgtt ttttcttttg
    10801 atggaaaaga aatatctaag gagacaatgc gtaaagcgat tgcaattgct aaagctttag
    10861 aggaaaatga atagttggag tgatggtttg tatgtatttg aaattgaaag aaatgttgag
    10921 cgaatacaat ttgaaagtag tttatatgga gatgaaagag cctggtttct actatccaaa
    10981 acccagaatc atttttttga atgaaaatct atacggtgaa actgcagaag cctttcattt
    11041 atctcatgaa cttgcacatt tcagcgcctc tcattttgaa ttttcagttt tgtacgatac
    11101 ttctacaact tttcactcaa aatttgaaac agaagcagat aaaatagcta ttctaatttt
    11161 attaaatatc tatattgaga acgaactgac tgatgaatct cagtttaatc tcgaaaaatt
    11221 tatggagtat tattctattc agaataagct cagatacact tgttatgctg tctgccaatg
    11281 ctattttaag aaaaaatatt cttacgcaag gcaatatgta tgaatacatc aagaattatt
    11341 aaaaaaaccg atttcagcaa aacattaaaa aagcccgtgc tgcaacacgg gctctttcct
    11401 catatatgag cttctacaaa aaaaatcata tcacagaaat gaggaagaaa aaatggaaaa
    11461 agagatttta ataaaatctc caggaaaaac cttgattaaa gtaactgatg attctatttc
    11521 aattattaga aaaggattta taaatttagt taatcaaggt attaaaggag aaaaaactat
    11581 cccctttaaa aatatatcag ccgtacaatt aaaaaaacca ggtatgagta atggttatat
    11641 acaatttacc ttgctaggcg gaaacgaaag tcgcggaggt atattagcgg ccacgaaaga
    11701 tgaaaacacc gttatgttta caaaaaaata ttggaatgaa atggaaaatt taaaaaaata
    11761 tatagagaag caacaatcta tattagataa taattccaaa gaaacacaaa tttcaagtgc
    11821 agatgaaatt aaaaagtata aagagttgtt agatgaaggg attattaatc aagaagaatt
    11881 cgattttaag aaaaaagaat tgcttggttt ataatattca atatcaaaag agaaagagga
    11941 tctattaatg gacggtatat ttggttttac tggta
//
LOCUS       DAZKLA010000087        11791 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000087 DAZKLA010000000
VERSION     DAZKLA010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.088"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG85N_002023"
     CDS             complement(<1..132)
                     /locus_tag="ACG85N_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0224757.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..841
                     /locus_tag="ACG85N_002024"
     CDS             542..841
                     /locus_tag="ACG85N_002024"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0224758.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            914..2275
                     /locus_tag="ACG85N_002025"
     CDS             914..2275
                     /locus_tag="ACG85N_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFQ0224759.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            2297..2557
                     /locus_tag="ACG85N_002026"
     CDS             2297..2557
                     /locus_tag="ACG85N_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224760.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2571..3323
                     /locus_tag="ACG85N_002027"
     CDS             2571..3323
                     /locus_tag="ACG85N_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFQ0224761.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(3417..4022)
                     /locus_tag="ACG85N_002028"
     CDS             complement(3417..4022)
                     /locus_tag="ACG85N_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0224762.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(4075..5049)
                     /locus_tag="ACG85N_002029"
     CDS             complement(4075..5049)
                     /locus_tag="ACG85N_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFQ0224763.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(5427..6146)
                     /locus_tag="ACG85N_002030"
     CDS             complement(5427..6146)
                     /locus_tag="ACG85N_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0224764.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(6273..7544)
                     /gene="celB"
                     /locus_tag="ACG85N_002031"
     CDS             complement(6273..7544)
                     /gene="celB"
                     /locus_tag="ACG85N_002031"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0224765.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7697..9094
                     /locus_tag="ACG85N_002032"
     CDS             7697..9094
                     /locus_tag="ACG85N_002032"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0224766.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            9175..9615
                     /locus_tag="ACG85N_002033"
     CDS             9175..9615
                     /locus_tag="ACG85N_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0224767.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9763..10089
                     /locus_tag="ACG85N_002034"
     CDS             9763..10089
                     /locus_tag="ACG85N_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0224768.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            10090..10839
                     /locus_tag="ACG85N_002035"
     CDS             10090..10839
                     /locus_tag="ACG85N_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFQ0224769.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10919..11374)
                     /locus_tag="ACG85N_002036"
     CDS             complement(10919..11374)
                     /locus_tag="ACG85N_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0224770.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3767 a         2058 c         2404 g         3562 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaagaaaa
      541 aatgaaaaat cggttaattg tcgcatgtgg aagtggagta gcaacgagtc agacaatcgc
      601 tagtaagatc caaagtatgt tagaagatga cggcatcgca tttcctgtag aagcagttga
      661 ttacaaatcg attcaaaatg agcttctaac tgctggaatc tatgtgtatg tcgcacaacc
      721 tgatgaggaa gtacttgaac aagcaaaaga tttgggtatt gaagtatttc caggaattcc
      781 atttttaact ggaatgggtg tagaacctat ttatgacagt atcaaagagt tagtacagta
      841 aaggaggatt gttggataaa aaagttggtt catagtttta tttactatat ttagaaagaa
      901 agaaggttta tttatggaag ttttccaaac cattatcaat tatgttttga acttaggatc
      961 agccattttt gttcccctga ttattttgat acttggattg ttggcaggga tgaaattaaa
     1021 aaaagcattc atgtctgctt tgactttagg gattgctttt agcggaatgt ctatggtcat
     1081 tggctatatg tctaacgcag taagtccagc atcagaagca ttggctaaaa atacgggaat
     1141 cagtttgcct gcacttgatc ttgggtggac aggtgcagca agcatcacat ggtcgtggag
     1201 ttatgctttt atattttttg cagtaacgat tggggtcaat tttgtactgt tactttttaa
     1261 ttggacgaag acgttaaatg ttgatatgtg gaatgtttgg gggaaagctt taactgctta
     1321 tttagtttat tatgtttccg gtagtttggc tgcaggcttt cttactgcaa tggtccaagt
     1381 tatattggaa ttaaagcttg gtgatatgtt tcaaaaacat atacaagatc tcacagggat
     1441 tccattagta acagtgactc attttatgac atctgccgct gtcttgttgc tgcctttcaa
     1501 tatgatcatg gataaaattc ctgctttaaa taaacgagcg gatacgaatg cattgaagaa
     1561 gaaaattggg atcttctctg aaaactctgt aatgggattc attattggat tggggcttgg
     1621 ctttgctgct gcgtatggcg tttcaggttc tcttaatcta gcgatccaaa cagctacagc
     1681 attaagtttg tttcctatga tctctaaaat gttcatgcaa tctttgtctc ctctagcaga
     1741 cgctatgtca gaatttatga aaaagcggtt taaggatcgt gaagtatata tcggattgga
     1801 ctggccgata ttagctggtc gttcagaaat ttgggttact gctattttac ttgttcctgt
     1861 gtttattggt tatgccatta ttttgccagg aaaccaagta ttgccattag cagggattat
     1921 caactattcg attgcggtcg gcggattact tttaacagga ggaaacttgg ctcgtatgct
     1981 gatcttggga atcatcacta tgcctattta tttgtacggg gccacttacc ttgcgccaat
     2041 actcagtgat ttagcagaaa aaagcggagc agtcgaggga atcaagaaag gacagttgat
     2101 cacatggtca tccatagaag ggccggagtt cagggtttta tttgcggagg catttaatag
     2161 gaatattctc gctattgtag gatgcgtgat ttttctagga ctgtttgttc tactttataa
     2221 atatatgata aaagcgaaag taccgagtga acgttatgct gaactacaaa aatagcattg
     2281 aaatggtggg aaagtaatga aaagagaaga gacagataaa ataaaatgga cagtcgctct
     2341 ttgtggtaca cttctgctat ttctatatgg actctttact cagaatatca tcatcaatct
     2401 attggtcatt tttttcgcac tagtgatcta taaatatgga aatcacgttt tattcagaga
     2461 gtacgatgaa aaaagaaaac ggaaaataga agaatcaatg aaaataaaag aagctaccaa
     2521 agaaatattg agagaaaaaa gttttataaa aagataatgg aggggtcatt atgtcagatg
     2581 gacgtatgtt atttgagaga gaacgacagg acatggcacg tatcgtaaga gtgattttcg
     2641 aaaggaaaaa cacaaatgtc gcaggaggga atttttcctt taaaaccact gatgatcaag
     2701 ggaaagacta catcatcatg acgccgacca tgatgtcaga agcctatcta ggagagattt
     2761 ctcctgcaca ggttctagta gtagaaccaa gaacgcgtag aatcgttgac ggagtcggtc
     2821 atttaactcg tgaaatcaat atgcatgaag cgatgtatga gactaatcca gctatcaaag
     2881 ctgtcctaca tgctcatgcg cctaatgcaa tgttctgggc gactactggg ttgcctatgc
     2941 ctaatttgac agaagccaca gctaaagtaa aagagattcc tgtcatggac tttgagccta
     3001 attgctctga agaacttgct gaattgacaa gcgactatgt aaaaaaacag catctcgaat
     3061 taccgcatat gttgctgctc aactctcatg gcgtattgat caacacaggt ggagaatcag
     3121 gaatcgaagc tatccataaa gcactgcaaa tccttgatac agttgaatgg aatgcagaaa
     3181 ttgcctacaa gcagacgatt tttcaagcat tagggattac tgacggtttt tattcaaaag
     3241 gtgagaaaat cgggggattg gaagagttga aacataaaac ggctatttat aataaatctg
     3301 ttattgcagc tggtggagac taacctatct gacacaaaaa actacgactt tcacaaaatc
     3361 tgatccagat cgatatctgc ttcaggttgt attatgtaag tcgtagttct tttcttttat
     3421 ttagttaaga ggtggtctgt ccattccctc gcttgcttga tttcgtccat cgtcaactga
     3481 tgacctgtta cagttagctg aatagtcaca tttgcttggc gtgtttcgaa ttgatcggcc
     3541 aattctttaa aggaagcagg ggaaacgatt ggatcgttat caccataaga aatccagacg
     3601 gttttatcct ctaatgtgga acggctttga tgaacaccta gagacatcgg atggaaaaac
     3661 aaccctttat ttagctcggt ctcacgctcc aataataaat gagcagcgat gtttgcgcca
     3721 tttgaatagc cgacaagcag ccagtcactt aacggtatca gttctttttc gctgatttct
     3781 ttgatttttt catggagttt atctgtttct ttttccaaac tgtccaggtc gaattgattc
     3841 aatccattac gcttgaaaaa acgattcatc ccatcttctt ggaccgtgcc acgaaaagaa
     3901 agcaatgtac tgtttggtgc aagaaagtga gcgatatcca gtaaagaatg ttcatcgcca
     3961 cctgtgccat gaagtaagat caaatgcttg ccacttgttc ctttttcaaa aatgtaggtc
     4021 attttgttcc ctcttttctg tttctataga tgcaaatgaa gtagcatgaa gagattattc
     4081 acgtttttta tttgcatcac ttgtatcgaa aggacggaca taagcttcaa tggcttcacg
     4141 acgttcttct aagaatggag gaagagatag tttttctcct gctgattctg ctggttcatc
     4201 ttcaaggaat ccaggaccat cagttgctaa ttcaaacagt acgtgatgag cggctaggaa
     4261 gtactcagat tggaaataga aacgatcgac gaaaccagaa tttggcaatt caatcttatt
     4321 gattacttga atccagtatt ccaatgcttc tttgtctgct acacgcaatg ctaagtgatg
     4381 gatatttcca aatccttcga ttgcttttgg caagtctgtc cgatgttcaa taatgatgct
     4441 tgcaccattt cccccttcac cgatttcaaa taagtagaaa tctcctgaat ggtctgtttc
     4501 tttgaatcct aagatttttt caagaatcaa gcggatatgc tctaattgac tgacacggac
     4561 aaagacaggt ccaagtcctg tgatcgcaaa ctctgtcggt acattgctgt ttttccaagg
     4621 aatgccagct gctacacctt gattgttttc gtcagagatc aattgataat actgattatc
     4681 gaaatcttca aattcgatat attttttgcc gaaacgttca ttgattgctg aatgagtaac
     4741 gtcatatttt tcaaaacgtt ccaaccaaaa gtctaatgct gcgtcacttg gcacacggaa
     4801 agaagcacga gagatcgtat ctgttccttt ttttcctttt ggctgatttg gaaaatcaaa
     4861 gaaagtcata tctgttcctg gcgcaccagt atcgtctgta aagtaaagat ggtaagtttc
     4921 gatgtcatct tggttaactg tttttttgat taagcgcatt cctaaaatat ctgtaaaaaa
     4981 gcggtagatt tttgtagcgc tagatgtcat tgctgtgacg tggtgaattc ctctgatttg
     5041 tgtatccatg ataaatttcc tcctgttaac ttacaaaaag taagtagttg atttagacat
     5101 agtatagagg gtgcatctct attttgcaat taatttgtct tctaaagtat caggaaggaa
     5161 tcaagaatgg aaaagaggag ggacggatca ataaaataaa aagacttacg actagctgta
     5221 tcgatttcaa aaaaactagc tttaaaaagc taactttaga atcattacgg gtggtcataa
     5281 gtctttttat gctgagttat tttttcattt ttgagctaat gcttaggata gaatagaatg
     5341 ttgatttcac gatctcttct tgttacttga aactgcttac cactgtcacg atctcttggt
     5401 aaacggcttt ttcacaacct ctttggttaa gttgaacgca catccagcac agaatagttc
     5461 cgaacatcaa atcggtttcg gctggaagaa tattcaatcg gctgtccatc tttgagatag
     5521 acgacttgct gaacttccag tacaggatcg ttttctgcac agttcaaata ttgctgatca
     5581 tattcatcac ttttatctgc agaaatagca cgataagccc ctgcaaaatg gacattcaat
     5641 tcttgatgaa gatgatcata gatagaggct aacaaatgtt ctttttttaa acccggcaca
     5701 agatggactg gcataaaggt atgctctaaa acatatggct cgccatctaa gatccttaac
     5761 cggtgaatat tgtagacagg ttgctcggca tcgatcatca atcgttcttg gatatattga
     5821 ttagggaatt caacatcaaa atgaatgatt tgactcttca aagaacgatg tctgcgtttc
     5881 atttggagac tcaaaccttc atactcgtta gcgggcgatg tgtctttttg cataaatgga
     5941 tgattcaaga ttttcgttcc tgatcctcgc tgcgaataga caagtccttc cattgctaag
     6001 atattgatgg cttttttgat tgtcatccgg ctcgcaccaa attcttttac caagtcgact
     6061 tgattaggca aaaaactatc gagaggatac tcttttgaac ggatacgttc tcttaaaata
     6121 tttgcgattt cttcgtattt tggcacgtta ttcacctctt ctagagtata acgaaagatt
     6181 atgcttccga caactcgaag tagcatctgg aacattgttt attcgaaaaa agaggtcatg
     6241 acaagttttt gtcacaaccc cttaatgatc tattaagcaa tatctaaatc tggttgttta
     6301 tctagtactt tcaggaatgg gtaccagata aaggcaacga tgacaaagtc gatgacttgc
     6361 agcataccac caagccatga atttgttgct aagacaccgc ttgcaatgat tggcacagtc
     6421 catggaacag agacgccagt tggtgcaggg acaagtccag cagccattac caaataattg
     6481 aacgtcgtta cgattagagg tgccacaacc caaggaataa ggattgttgc attcaatacg
     6541 atcggtagac cgaagatcgc tggttcattt acattgaaaa taccaggtgc taaagctaat
     6601 cgtccaacat cacgatactg tttcttttta agaacaaagg ccatcaataa aacgacagct
     6661 aaagtcattc cagagccacc aataccaaca gtgaaggttt ccatgaatgg ttttgtgatg
     6721 atgtgtggta attcttttcc atgttgataa gcttctaagt tatcaagcat cagtgtattc
     6781 caaatcgggt ccattacaga gttgacgatg atttgtccgt gaagtccgaa gaaccataag
     6841 aattggacaa agaacaaagc aatcaatgtt gcaggtaaac tacttcccaa accagttaat
     6901 ggtttttgga tcacttcata aacgacgtca tgtaagttag cattgaaaat accagtcatc
     6961 acagcattga ttactaagaa aatagttagt gtcactacag cagggatcaa agcggcaaat
     7021 gattttgtta ctgcaggcgg aactccatca ggcattttga tctgccagcc gcgtttcgtg
     7081 attctgcagt agatttctcc agccaagaaa gcagcgatca ttccaatgaa cattcctttt
     7141 gcgccaagac gatcaagtga aagcacacca gtcacatctt caccgccagc ggttttcatt
     7201 gcaaaaggcg taagaatcaa gaagctggca aaagacacgg caccaccgaa aataccttca
     7261 acgtcataag attttgataa ataatagccg ataccaaaag taacaaaaac cgacatgatg
     7321 ctcattgttg cattttgccc attaccgaat aaattcgata aagttccctt tgttgcgtca
     7381 ctgaaaaaag gcaagttatt gataacgaca acgatcgaac cgaacatcgt taaagggaag
     7441 gaaagcatga aagcatcccg cagcactgta aggtaacgat tttgccctaa taaattagct
     7501 aagggcatga ttttctcaga aagcttgtcc ataaaaccgt tcatttttat tcacctcaaa
     7561 tttatttgca atcgattaca tacaaagtat aacgccaaaa attttaaatg tctagtcatt
     7621 ttcataaaaa gtattgattt attttttagg gttcgttatg attacggtat caagaaaaaa
     7681 gagaggaagt aaaaaaatga aaacctacac attcccagaa aatttttggt ggggttctgc
     7741 tgctagcgga ccgcaaacag aaggaagagt aagtgatgat ggtaaaggag aaaatatttg
     7801 ggatcattgg tatgaattag agcctgaaaa attcttcgat caagtaggac ctgaaaaaac
     7861 ttcgcaagtt tacacaaaat acaaagaaga tgttcagtta atgaaagaaa ctggccataa
     7921 cacatttcgt acatctatcc aatggagtca tttgattcca gaagggacag gtgaaatcaa
     7981 tcaaaaagct gtttcttttt atcgagatta ttttactgaa ttgctggata atggtattga
     8041 accttttgtg aacctttacc atttcgatat gccactacca ctgcaagaaa aaggcggatg
     8101 gttatcaaga gaaacggtcg acgcttatgt ttcttatgca gcagcgtgct ttgaactgtt
     8161 tggagatctg gtcaaaaaat ggtttactca taatgaacca atcgtacctg tagaaggagg
     8221 ctacttgtat caattccact atccaaatca tatcaatatg aaagaagcgg tccaagtagg
     8281 ctatcatgag aatctagcaa gcgcaaaggt aatcaaagcc taccatgaag gcggacatga
     8341 tggtgagatc ggaatcatct tgaatctgac accaagctat ccgagagatg aaacgaaccc
     8401 agaagatgta aaagcggcga agatcgctga cgcattcttc aaccgatcat tccttgaccc
     8461 tgcagtgaaa ggagagtttc cacaagagtt gatcgaaatt gtcaaagaat tggctataat
     8521 gccagagatc catgaaggag acctagaact catccgtgaa aacacgattg acctgctagg
     8581 aatcaattac tatcagcctc gtcgaatcaa agcaaaagaa actccaatca atcacgaaca
     8641 tagccccatg ccagatgatt attttgataa ttacgacatg cccaataaaa aaatgaatcc
     8701 atatcgtgga tgggaaattt acgaaaaagg aatctatgat attttaacga atacaagaga
     8761 aaactatggt aatatcaaat gctatatttc tgaaaatggg atgggggtcg aaggagaaga
     8821 acgatttgtt aacagcgaag gaattattga agatgattac cggatcgagt ttgttacgga
     8881 tcatttgaaa tacgtccatc aagcaatcca agaaggaacc aattgtgtgg gttaccacat
     8941 gtggacatgt atggacaact ggtcttggac aaatgcttat aagaatcgct atggctttat
     9001 cgctgttgat ttggcacaag acggtaaacg gacgatcaaa aaatcaggtc gctggtttaa
     9061 aacggtctct gaaaaaaacg gatttgatgc ctgatatttt ttgataaaac gttcacaaaa
     9121 tgtttgttct aacactgtaa tcgtgctaca ctaacagtga aggaggcgtt tacaatggta
     9181 gatacttatc gtaatatttt agtagcaatt gatggttctg aaaaggcaga aaaagcattt
     9241 cttgaggcaa tcacgattgc caaacgaaat caggcaacgt tgcacatcct gtatgtcaat
     9301 gaagtaacag gcaattactt tggtgatttt gcatttgtta caacaagcct tcaggaagaa
     9361 ttggatgagg ttgcggaaaa ccagatgaaa gaacatcgaa acttggcaat tgaaaaaggc
     9421 ctgacagata tcgagacata tgttctttac ggttatccaa agacactgat tgctaatttc
     9481 catgaatcga aagaaccaat cgatctgatt gtcatggggg cgacaggtct gaatgcggta
     9541 gaacgtgcac tagtaggttc cactacttcc tatgtagtca accacgcatc atgtaacgtc
     9601 atggttgtaa aataaataaa ttaagggaac agaaagcaaa aaagcataac gcttcttttg
     9661 ctttctgttc cttttatttt tttgtaaaaa attactttac ctgatgaagt aatcatgcta
     9721 ttctaattac aacgacagat ggagtaatat ggaggaaaga aaatgatttc aagtgatgga
     9781 atacgcggat acaatgatgc gattatttta tcgatcctta acaaaaacga ttcttatgga
     9841 tatgaaattt ctaaaacgat tcgtgagagg acaaatgatt cctatgtgat caaagagaca
     9901 acactttact ccgtttttgc ccgtttagaa aagaatcaac tgattgtttc gtatcctgga
     9961 gaagtgactc atggaaaaaa gcggacatat tataaaatca ctgaaatagg gcgtaatctt
    10021 ttagaagaaa aaaagcaaga gtggctgacg acgaaagacg tagtcgatca atttttggag
    10081 ggaaattaaa tggatacaat ccgtaattat ttagacagtc tttttatcgg agtacctcaa
    10141 agtaccgaga tagataagct gaaaactgat ttgttggcaa atatggaaga ccattatcat
    10201 gaattgatgg gagaaggaaa aaatgaacag gaggccatcg gtacagtaat ttcgacattt
    10261 ggttcaatcg atgaattatt agaagaacta gatgtcgaaa aaaaacatca agctgacgag
    10321 acggaaacaa acacagcttc tatttattta agcgaagcag aaaactactg gaaagaatac
    10381 agagcggcaa gtctccaagt ggcatcaggt gttctattca ttagcttatc ttttgcaagt
    10441 tttcttttct tttgttcagc aggatatgtg ttcatgggta tttcttgtct gattttcggt
    10501 atagcattag ctgtcggttt ttttattgct tctggtatga aaatcacacg tttaaatcac
    10561 tttttgcacc atcggaaaat acctgaaaaa gtattagcag aagcaaaaga aaaagaagaa
    10621 gagtatcagc gaagttttgg cttctcactg attgcaggaa ttggattatg tattttttca
    10681 cttttcccac ttttggcttc tttaatgtgg tatatggatg gtagtatcgg tgcctctata
    10741 ttttttgtta cggtaggaac gggtgtcttc ttgatcattt atggcagtct agtccgtcat
    10801 tcttatcgtc aattcactca aagcgcttat tactggtgaa tgtattcaga tttgtaaaaa
    10861 tattctattt atccggcgta gaaaaaaaga ctgtgacacg cttttgtcac agtctttttt
    10921 atatataata atttaaaaat tcatcatcta aatccgtaga attttctaga ttgcttaccc
    10981 atgtaaaatc gttatagaca gatttcagtt gacttctgcg ccattgtaca gggataaaaa
    11041 gacgcatgct atcttcattg aatccgacta ataatttctt tccgtcagta atgatgggac
    11101 gttgtaacaa agtaaaatgc gagacgatca gatcgacaag ctctgaggtc gtacattggt
    11161 cgatttggtc tttcaaattt ttataatcaa tcgagcgttt agctaataga tcgtcaaatc
    11221 cgttttctgt taatgacaat aaataaaata tctctttcct tgttaaaggc tgtttaggtg
    11281 tgaaaattcg ttctctgtaa ggcatttgat ggtctttcag ccagtttgcc atcagatggt
    11341 aactatgtgt gcgagaagaa tagatcgtaa gcataaaaaa actccttaaa tagtccatta
    11401 atttgttaga tctgttgaga taaataatag gtaaggagct gtgcataagc attgatcaaa
    11461 acaacccaaa catcattaga aataactttc taaagtctta aacttgcgac gagaagcaac
    11521 tagactaata gaaaaaatgt cttagccatt tggcttcctg tttgtttttt tgcttatgct
    11581 gcagctcctc gggaaagggt tctaacaatt tgaagaaggt tgatgattga tctagtttaa
    11641 attgtgtgaa atttgaaatc tggtgttttt actagatgaa tcacctaaca actatgatta
    11701 taacttatag aacttctgtt ttgcattcgg ttttgttata gttgttataa agttttattt
    11761 tattacagaa catttttaat taataaagaa c
//
LOCUS       DAZKLA010000088        11764 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000088 DAZKLA010000000
VERSION     DAZKLA010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.089"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..517)
                     /locus_tag="ACG85N_002037"
     CDS             complement(140..517)
                     /locus_tag="ACG85N_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224771.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(817..1698)
                     /gene="galU"
                     /locus_tag="ACG85N_002038"
     CDS             complement(817..1698)
                     /gene="galU"
                     /locus_tag="ACG85N_002038"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0224772.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1741..2760)
                     /locus_tag="ACG85N_002039"
     CDS             complement(1741..2760)
                     /locus_tag="ACG85N_002039"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0224773.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(3393..4397)
                     /locus_tag="ACG85N_002040"
     CDS             complement(3393..4397)
                     /locus_tag="ACG85N_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0224774.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(4410..5459)
                     /locus_tag="ACG85N_002041"
     CDS             complement(4410..5459)
                     /locus_tag="ACG85N_002041"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0224775.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(5476..6297)
                     /locus_tag="ACG85N_002042"
     CDS             complement(5476..6297)
                     /locus_tag="ACG85N_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0224776.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(6284..7063)
                     /locus_tag="ACG85N_002043"
     CDS             complement(6284..7063)
                     /locus_tag="ACG85N_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0224777.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(7080..7550)
                     /locus_tag="ACG85N_002044"
     CDS             complement(7080..7550)
                     /locus_tag="ACG85N_002044"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0224778.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7562..7981)
                     /locus_tag="ACG85N_002045"
     CDS             complement(7562..7981)
                     /locus_tag="ACG85N_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0224779.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(8062..10839)
                     /locus_tag="ACG85N_002046"
     CDS             complement(8062..10839)
                     /locus_tag="ACG85N_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0224780.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            11030..11254
                     /locus_tag="ACG85N_002047"
     CDS             11030..11254
                     /locus_tag="ACG85N_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224781.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3408 a         2480 c         1905 g         3971 t
ORIGIN      
        1 tatcccgata caacaagcat tcctttaaaa aagattcttt tatataagtg aaaaaagctt
       61 ttttccacca attaaataaa tctctcatct caatagttta tccattattt tatctttttt
      121 tgcgtatacc tactaaatat taccgtcttc ttctcgttct tctactttgt tcgagagaat
      181 ctgtactaga aaaagtagtt gtatgtctac cttttctgcc tatccgagac tttcttttca
      241 tatatgggat ataaatgatt agatacagta cagcaaaaaa taaagcaaat acgcaagctt
      301 cccaactacc taacgccaat tcaaccatca aggtagcaac cccaacggat gctgcatacc
      361 agataaaaat cagaaacgtt tctttttttc tgaccatgcg aaacatgata aatagactaa
      421 taagcaatag tggcaacaca aatatccaat tgcttccttc catcagccac ttgagtatat
      481 caagcagcga ctgtgcaaca gcgactaatt ttcccatagt gaacacacac ctttttttat
      541 aattaaaaaa ttcgcctttt tattctacta tgaatcttct ggcttggctt tcatttctct
      601 tatgtaatca ttttctttta tacaacttaa aaaaaaatta aaccattagc ataatatttt
      661 ctgaatctat tcacgaaagt ctttttctat ctttcgtttt ccacgactca actgatctag
      721 agtcaaaaaa ctaaaacgac agttgcgcaa atgacgcaca agctgccgct ttaataacaa
      781 atagcctttc attctcagca ggccacttat ttccagttaa ctttctgttc ttagcttttt
      841 actcaactgg ataatatatg cacttaatga agtcccaatt tccgaatgtt gaagtccata
      901 ttcaatattt gtcttcatat atcctaactt atcgcctacg tcgtatcttt ttcctttaaa
      961 ctctctcgca tagaccgcct ctgtctggtt aagagtctga agtgcatccg ttagctgtat
     1021 ctcgtttcct gctcctggtt cttgagtttc taatatctca aatattttag gagtcagtag
     1081 ataacgtccg ataatggcta aattacttgg tgcttttccc atttcaggtt tttctacaaa
     1141 cccttttact ctgcaacgac ccttttcgct atctgtttta ggatcaataa ttccgtattt
     1201 agatgtttca ttttctggta cacgcatgac ggcaacagcg gaagcttgtg tttcttcata
     1261 atccatcatc aattgttgag tcaatgggat atcatctttc atcaaatcat cccccagcat
     1321 gacgacaaac ggttcatttc ctataaatgc ttttgcttga agcacggcat cccccagtcc
     1381 tcttggatga gattgacgga tgaagtgaag attgacttga gtcgtttctt caactaaagc
     1441 gagtaattct gttttttcct tttcccgcaa attgttttcc aattcgatat ttgcatcaaa
     1501 gtgatcttca atgggtcttt tttcttttcc cgttacgatc aagatgtctt cgattcccga
     1561 atccaatgct tcttccacaa taaactggat cgttggttta tccacgattg gaagcatttc
     1621 ttttgccatg gcttttgttg ccggcaaaaa acgtgttcct agcccagcgg caggaatgac
     1681 tgcttttttg accttcactt gctgttcctc ctaaacgacg attaactatt gatcatctat
     1741 ttaacgaaga gaaaattcat tctccatttt tccgtcacgt aacatgattt cttttgctgc
     1801 ttttttgata tcttctccat tatacaaaac attatagatt gtttgagtaa ttggcatctc
     1861 aacacctagc tgttgagcta gctctactgc tgcttttgtc gttgccacac cctcaacgac
     1921 cattcccatg ttttctagaa cttcttccag cttatgtccc tgtcctaaca agttaccggc
     1981 acgccaattg cgagaatgaa cacttgtaca agtaacgatc aagtccccca caccactcaa
     2041 accgataaat gtcagaggat tggcacccat cgcgacacca agacggctga tttctgccaa
     2101 tcctcgagtc atgattgctg cttttgcatc atctccgaat cctaatccat ggatagcgcc
     2161 ggcaccaatt gcaatgatat ttttcagtgc tgcaccagtt tctacgccaa tcacatcatt
     2221 atttgtataa atcctgaaat aatcattcat gaacagtttt tgtacatagg ctgctgcttt
     2281 ttgatcaata cttgcagctg taattgttgt gatatcatgt actgccactt cctcagcatg
     2341 gctaggacca gataatacaa caattgcctg tcttttttct tctggaattt cttcagccaa
     2401 aacttctgag attcgtttat gcgtatcttg ttctagtcct ttacttgcat gtataatgat
     2461 tggcttagta ttcattttac caataagttc ctgtgcgact gaacgaatcg cttttgttgg
     2521 aacaacaaat aaaatagcat ctgcgttatc cactgcttct gccaattctt tatatccgat
     2581 aatcgattct gggatcgtca aatcaggtaa aaaatgctga tttgtatgat acttattgat
     2641 ttcatcaatc tgttcaggta catttcccca aatacggaca tcatgcccgt tttctgccaa
     2701 tgtttgtgct aaagcagtgc cccaagatcc aggaccaaga actgcgattt tttgtttcat
     2761 ggatattcct ccaaataaat aattgataat ttccattata gtatacattt cttaaagttg
     2821 ggactaattt ccattgtttt taagtgtttg tttacttctt ttatcgtttt tacttgaaat
     2881 aatcaatgat atgttacaat agtctacatt ttatttttac actcgttatg ctttgcttgt
     2941 tcctaagagg aagcagtttt gacaatgtta ctaccagcat tactattagg aggttaggaa
     3001 acttctccta gtgagtattg atttaaaata cataagagac agattgaaca tttctaaatc
     3061 tgtcaggtcc tgcaacggac agattgtctc tgttgacggt tgacatagga acttttgttt
     3121 tagtcaacat tctggataca aaacgctatg taaactggcg caactcgttg tacttggact
     3181 tttactacgg atccatgcaa caactaaata gtactattgc ataacaaaaa atggttggca
     3241 cccacatgtc ccattaaaca gattataagt tgaactgtag ataatgcaaa aaatgtgttt
     3301 tataacgtaa ttgatgccaa tattttaaaa aaataaaggg agcagttatc gctgctctct
     3361 ttatttttta tattcttttt atccagtcta tcctaataat caaactgacg atagtagcgg
     3421 cgtatctcta atggatggca gttcaactgc tccacatgag catcgatgcg ttgtgtcact
     3481 acatgcatca ataagaaaga caaaacacgt ccgcgatatt tttcactgat accttctact
     3541 ggatagtctt ttgtgtctat caatgtataa ttgctgcaaa tttttggcaa aagtttagcc
     3601 acacgttcag ataacactct ctgttcatct tcgcctaaaa ataatgtgac tggggttgtt
     3661 tcttctacga tctctaacgt cccgtgaaga aactcggcag catggataga tttcgatgga
     3721 agccagcttt gttcttccca gtaacacatg gcatatgaat aaacagctcc ccattgattc
     3781 cctgctccaa taaaataatg catactatca cgtcgatgtt tttctgcaaa tgataagcca
     3841 aatgcatctg cttttttctc tgcttccact aaccctgttg gtaaataacg ttcgagctgt
     3901 tcatagtatt ctgaataagc ttcgaattct ccattcaaat acatcaatcg atctgctgcc
     3961 ataaaaaatt tgatttgttc tgttccagga gctggataag tgaccacaaa atcgcatagc
     4021 tgagacaact tactgttcgc tttatcgata aatccaatca gtgtcgctcc tactttcttg
     4081 atttgttcta ctgctttcac tacttcttga gttgtaccgg taacagagga caaaataacg
     4141 atagaatctt ttgttaatct tttgtttcca gtagtgtaat attctgcagc atgctgtaca
     4201 aagacaggca agttgctttt cccattcata taagtgacag cttgcatcga ggaagcatac
     4261 gttccaccga ttcctagata atagacagcg tcaaattttt ccgcataaag tttatcgatg
     4321 atttcttcta cttgcggacg taaagctagt gcgcctttta ttgctttgat ttgttcttct
     4381 tcattgaatt tcagcatgat ttccacctct tataatttac ttttcaagac tgtatcaaag
     4441 tcttcaaaaa tctttttgtt caagtcaata cctttagcta ctgcggtttt ataagcgaat
     4501 agttgaaacg gaacgacaag cgctaataca gacagatatt catcacattc tattgctaat
     4561 ccaagtgttt tctcgtttct tgcttttttt gtggtaatgg tgagagtctc tccaacgtac
     4621 cgactcatat atctttgcaa cttttgcgaa cgttccttat ttacactttc cccttcaata
     4681 aagaatagca aatgcgaagc atctgcctcc aggtatgggc cgtgcatata agcttctaac
     4741 tcaaatcctt gtgacggaac gcggattgtt tcagtcaatt tcgtctctgc ttctttagct
     4801 gttccccagt ttggaccata tccaatggtg acgaaacgct gtgctaaacg aaataaactt
     4861 tgatatccat caaaaaattc ttccgttttt tgaataatcg ctggtaaatg gttgattatt
     4921 ttgcgcaact gttgcatgta gtcttgttct atttttttgc tgattttatt tttacttatt
     4981 ccaattccta tccctaagag tagtaactgt aaaacagtcg cagaatagcc ttttgtaacg
     5041 aagcctactg attctacccc ggcacctaaa tcaataattt gtgcagcttc taaagcaagt
     5101 ggactttgca cgtcatttgt gatagcaaca acaggtaacc cttgtttttg tatcattttt
     5161 actgcttcaa cagtagaagc acttttcccg ctttgtgaca cagcaatcac agtatcgatt
     5221 gaaggagaaa gttttccata attgaaaaaa taaaaaggtt cttgaatatc gacagtaaga
     5281 tcaccataag attccaatgc tggcttcgct gctaaacaag cgttataaga tgaacccgtt
     5341 gctaagatca gtaaattcac tgtttttttc atatgttctc ttgtcgaaaa atcattcttt
     5401 ttcaagatcg tacttagtac ttccttttct tcatagatat aatcttgcat cgtaaacatt
     5461 ttctctgctc cttttttaaa aaatccctat ccacgaacca aaaattccaa agaaagctag
     5521 tccgatcaag atcgttaatg gtttcacttc tttcttcaat agataaaaca ctatgccaaa
     5581 tgctccaaga cataagatat ttggaacgat atcatcaaaa atactttgaa cagtcacagc
     5641 attttctcct gaaccgattt ttaaaggaat attgatggtg atcatcgtgg cagtcattgc
     5701 tccaacgacc atcagtccaa taatagacgc gcctaatgtc aaactttcca tcataccgtt
     5761 tgcctggatc tttttcaaaa aacccgttcc taagcgatat ccccaacctg ttgctagata
     5821 gcgaaacaga atgtgcggaa tattgaaaat caaaatgaac aagatagggc ctaaaatatt
     5881 tccttgcatg gctaaagagg tacccacacc tgtggcaatc aatcgcaaag ttccccaaaa
     5941 gaaagaatct cctaaccctg caagaggtcc catcaaagat gttttgatac tatcgatcgc
     6001 agtcacatca aattcaggat catccttatt ttcttcctcc attgcagccg tgattcctaa
     6061 aatcagtgta acgatatgtg gggtcgtgtt aaaaaactct agatgccgtt tcaaagctgc
     6121 tgccatttct tctttcgact gatataactt tttcaaaatc ggaatcattg cataaacata
     6181 agccatattc atctgacgtt cataattcca agaaaactcc atttgaaaag aacgccaaaa
     6241 tacgtgattt agctcttttt tcgttatttt gctgtttttg atctcagaat tcttcatctt
     6301 cttctacctc ccctgcagtt gtttgaagtg gtagttgacg gacattcata atattgacta
     6361 ccacaactgc taaaatcgca ccaaaaatag ctactccggt cacaggaatc tctaagtaag
     6421 aagctatagc aaaacctaag aaaaaatatg gtgcaacttg tttgttgatc agcaaacgag
     6481 caagcatcgc aaaaccaaga gctggaatca accctgtagc tactgacaac cccttttgaa
     6541 cgaagtcagg aatcatattt aatagatttc cgatcgtatt gcttcccacc atataagaaa
     6601 ccattaccac aacaccaagc atcaaggaaa gaccgatacc ggcaagcaag tgcatatgct
     6661 cgactccttt ataatttcct tcttctgcat aattgtctgc tttatggttc ataattggaa
     6721 tcaaaagccc taagtaaata tttttcaata tcaatgtcaa cgtagcaatc ggcaaaccta
     6781 gtaaaagcgc ggtttctgta ccggctccag ctgtaatagc aaaagccgtc cctaaaatcc
     6841 caccggtcac aacatcaggt ggaatcgaag cacctactga gaacgatccg ataaatgcta
     6901 actctaaagt tgcgcccata atgatcccag tttttacatc acctaacacg attcctgtaa
     6961 acagccccgt cacaatcgga cgagaaagca acgatgtccc tagtgcatat tctgattgtg
     7021 cgacaaatgc aacaattcct aataagactg cttgtacaat cattgaggtt cttcctttct
     7081 tacaaaactt ctcttacatc cactttttta tctgctggta cttgacggat ttctacttct
     7141 atttgtttgt caattaatcg tttcaagcgt tcctcctctt ctggtaaaat atttaccgct
     7201 tttccgatgc tgcgagtacc aggttttgcc ttgatccctc ctaagttgat ggttttgatt
     7261 tctggatacg cttctgccag cttatatgca tcctcgatcg attccactac gatgaataac
     7321 ttatatttat ctgtcacccc gcttttcaac gcggcaatcg agtcatcaat tgatttgatc
     7381 actagtttga ctccttgtgg cttggctaat ttgatcgttg tctttcgtat ctcattcgta
     7441 ggtacatcat cattagcaat caaaatacag tctgcgccta ttgtttgtgt ccatgaaaaa
     7501 gctacttgtc cgtgtaataa ccgatggtcg actcttgtca atacaatcat ttttcttcct
     7561 cctaaaattc gtcttcttct attttctgat tgagccgctc gttacaaaac tggatcattt
     7621 tttttgaatc agttagcacc tgctggatca aaacagttgt ctctacttca gcgtccaacc
     7681 gagatactag ctcaatcaat agtggcaaat tcaatccggc aacgagatag acatgatccc
     7741 gattgatata ttgtaagaat tcgttgttga cacttccgcc aaaaagatcc gtcagaacaa
     7801 tgatttcttt atctttgtac ctcataaaaa gctgccttac tgtttccgct aaatcaaaat
     7861 tctcatcagt atagcaatcg aatacagtaa tagaatgatt tttcccatag atcagctcta
     7921 atgaatctaa tatccctgaa gcaaattttc catgggaagc taagataatt tctctatcca
     7981 ctttttcatc tcctcttttc acttacacat actaaaaaag caagttgcat gccaaaatta
     8041 gtggcaacaa cttgcttatg ttcaaaattc ttcttctatc gagagaaact ctgtatcttc
     8101 actcaaaata tcatagatat aggcaatctc agaattgggg atcatgacac tataatgctc
     8161 ttcaatgaca ctaaacgcag atttaatagc agataaccct tctttttgac actgcttcca
     8221 tttctctaat ccttgatagg tttcgattgg catttgtcgg atcaatcgtt caatgagaca
     8281 gctcacatgg acatacaacg ctaatttttt gggattgctg atgcttcgat tcaactgcac
     8341 ctctaattct ctcataaaat gatcgatttc tcccatcacc ttctctgtat ctagaatagt
     8401 cacggaatcc agtacttttt ccaatgaaaa attacgaatg atgcgctgat tgaatatttc
     8461 cttctctgaa gcattcatag tagtgtccaa ccattcagct agtacagaca tcttttcccc
     8521 cgagatgagg tcttctaatg aaagatagtc gatattggtg atatcaggat ctgtggtgcc
     8581 aatcattcct acgacttcgt atatggaaaa taatgggtca ctgtttttct tatcttttaa
     8641 ttgctgatat tcatatggga tgattttcaa ctgacaagcc gtaggtaagc ttttttctag
     8701 cagttcactt aagtggaccg ccgtaccgat tccagtgaag caagtagtaa tgagtgcttt
     8761 gggtttgttt tcctctggat agagcagtcg ccattctggc tggatctgtc gaacagtctc
     8821 ttctgctatc tcttctaatg atacattttt ttgcaggcat tcccctactg tgattgctaa
     8881 aggtgtcgtc acattattca aaatcaaaag cggtacggat aactgttttt taaaaaactg
     8941 atggatttct ttcagagatc ccatgtctac taatatgatc aatccatttg aaacatcgct
     9001 gttttcgcaa taatcaagga tttctgaagc aatctgctgt ggtgtgacat ctagtggcat
     9061 atcaaatgat tcaaatagat cctcttgtaa taaacgattt actacatttg ccatactgct
     9121 agcagttgca taaccgtgag cagcaatcat cgcttttggc agtcgttttt cttttgacca
     9181 tttcgctttt tttagataga tcgtcagtaa aatccgatcc atacttgtgt cttcaatatc
     9241 taatttgatc ttacttaatt ccagaattcg ttggacatag tgataactag atggataaga
     9301 ctgagaaact tgttgttcta attctttgat tagttggata atatcatgtt cttccggtaa
     9361 ccatttgata gtgcttcgct gatagagata gtaactcaag gcatagacac tgtttccatc
     9421 gaatttgatt tgataggcag tttccatttg tcgaaaggtc tctcggatag attgtgtcca
     9481 ataaaccagc agctcgtgtt tttcttgtct actggtctca aacagcaaaa aatcaaataa
     9541 ttgaatcact tcttgcttta attttccttc gcaagaatat agcagacagt tatttttctt
     9601 gaaatcaatt aggatttgct caaaactttt aacgattcgc tgctgttcgg gctgttgctt
     9661 ctccattaat tgctctaagg tctggtcttg cgtaatatag attccttgct gtgctgtatt
     9721 tttagtaata ggctgcgcaa gtagttcttt ttgcaaatga tggatcgtga catggatttc
     9781 aggctgttca cgctgttcag catatgcttt tgctgctgaa actttgacga tatttttcag
     9841 ctctcctatg tttccggata atttctgact tgttaaaagt gacaaagctg gtccactgac
     9901 tgacaaaggg ctccccattt ttctttgttc ttcgagaaaa aaagagtaaa tcagttctag
     9961 ccgttcatta cgactacgct gattaagtgc aggtagttcg attcggatag gtattcttcg
    10021 aatgaaagtt gttaagaaat gactagtaat ctcttcagtc gtagcaaaaa agagacgtgt
    10081 tttgatccgg cgtgacaaat taggttctcc catacgataa atcacacctt gatcaagata
    10141 ggtgaacagt ttttcctgac cttctggtgt taagcgatgt acctcgtcta aaaacaagat
    10201 cccaccatct gctgcttcaa aagccccttt tttattttct tctgcaccag tgtacgctcc
    10261 cttaacatgt ccaaacaaat tgctagtcaa aagttcagga ttatctgcat attgtgcaca
    10321 attaaaagtg atgaatggtg cagaatcttc tagcagatca tgaagcagac aatactgata
    10381 gaccaaatta actaaataac ttttacctgt gccactttcg cctgtaatca aaagtggtaa
    10441 tccgccatct ggatagtaaa gagctgtttt gatttgttcg atactttctt tcaaactggc
    10501 gtcgtatccg atcataaaag agaataagtc ttttggttgc gcgaaaaaag gctgttcctc
    10561 tttgagatca tcgatagaaa aataaaaatt cgtgcgtaac ttaaaaaatt gtttttcaaa
    10621 agcttctttg tggaaataaa tcactggtcg agaattgatt ttgactagta gttgctcatc
    10681 atttaactgg ttcaaataat gactgactgt gtttcttttt accccaaaat gatcagctaa
    10741 cctttgtgct gtaaagacct cgttcatatg ttctaaatcg aaaaaggctg tctggttctc
    10801 taagtaacat aaaatttcct tcttcaacaa cttattcacc cctcctccat catagaaaac
    10861 ggattcattt tgtcaataaa atctttaata aagaattgaa aaatatatct tattatagac
    10921 agcagcctac ttatccctta taatttttat tctaaaaaac gtacagttcc ttccagtttt
    10981 tatactggaa tttcaacata tgaaagaata aggaggctat gactattcaa tgaaatcgga
    11041 taaatataaa aacaggaaac atacttcctg gaaaactttt cgagaaatca tcggatctta
    11101 tctcgctgta gttgtatttt tgcctttgct ttatatggct atgacagaac aaactcgttt
    11161 tattggcgta gatacgcaat atatagccga aacaggattt ttattcatct ttatcttgat
    11221 ttttgcgttc tataaatcct tccatacaaa ataagattca cagatttcaa ctttcctgtc
    11281 tgtttttagc tattacaaat ccttatatgt ggtataacaa caatttattg gcataatgag
    11341 gaaaaacgag ttatgatgct ttcagagagt aaagaggtac tgttgtcacc atccttttta
    11401 ttgattacgt acaccactct tagttttttc ccattgatta cgaaaacaag gtagaaacat
    11461 tgatgttcct accttgtttt ctttttttat tttcagatca aaatctcaag tagcttttga
    11521 cttgatgtcg ggaggttgtc ttcgttccat caaacctgat agaatctgcg ccaaaccata
    11581 gttttctcca ctattattta ttcctctaag atagtaccca caataaacga ataaatattt
    11641 ttctcatttt catactcctt ctttgaaaat caattagatt tctctcaacc cactttttta
    11701 caaaaattac gtagcaaaaa agattgcaag aacaaattaa aaaagaagta aatagagttt
    11761 catc
//
LOCUS       DAZKLA010000089        11647 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000089 DAZKLA010000000
VERSION     DAZKLA010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.090"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2563)
                     /locus_tag="ACG85N_002048"
     CDS             complement(<1..2563)
                     /locus_tag="ACG85N_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFQ0224782.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
     gene            complement(2785..3669)
                     /locus_tag="ACG85N_002049"
     CDS             complement(2785..3669)
                     /locus_tag="ACG85N_002049"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0224783.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(3764..4201)
                     /locus_tag="ACG85N_002050"
     CDS             complement(3764..4201)
                     /locus_tag="ACG85N_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224784.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            complement(4418..5329)
                     /locus_tag="ACG85N_002051"
     CDS             complement(4418..5329)
                     /locus_tag="ACG85N_002051"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0224785.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(5706..6344)
                     /locus_tag="ACG85N_002052"
     CDS             complement(5706..6344)
                     /locus_tag="ACG85N_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224786.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(6348..8330)
                     /locus_tag="ACG85N_002053"
                     /pseudo
     CDS             complement(6348..8330)
                     /locus_tag="ACG85N_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            8510..9175
                     /locus_tag="ACG85N_002054"
     CDS             8510..9175
                     /locus_tag="ACG85N_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0224787.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(9572..11605)
                     /gene="brxL"
                     /locus_tag="ACG85N_002055"
     CDS             complement(9572..11605)
                     /gene="brxL"
                     /locus_tag="ACG85N_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFQ0224788.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
BASE COUNT         3566 a         2385 c         1807 g         3889 t
ORIGIN      
        1 ggatagaaag aacaaattta cctgtatcat caatagttgt ttcggacaaa acagcttgat
       61 tttttttcaa tgaaaccaaa gctcctgctt cgcctgtacc tgtgattgag cgagaagctg
      121 cattgatcaa tgtggtactt aatttagcag gctcaggagg aatcacatcg actgtcgttt
      181 tggcagagtc cagttctgtc acttcttggc cgcctcttac ttttttagca gcaactattt
      241 gcacttgatc ccccgttgtt agtctttctt cgttgggtac ttgaacctca aacaaaccgc
      301 cttgttcgcc ttctccccac ggattatatc ctgtcccttc attttcatct gtttgtcctt
      361 gtgtgagagc agtaatttct acaggtgatt tcgtcgtatc tgcataagta atacgtaaag
      421 tcacttctac ttcgcctgtc caagcatctc ttggctttgc ctcgacacct tcgggaacaa
      481 cgacatgccc atagattttt ttatctgcat tcgttggtac tcttaaggat tcaatcatcg
      541 gaagctggtt attggcactg attcgattat aatttttcat tccggcacct gtctgagcaa
      601 attcatttgt taaacctgca ctagggcttg atacaaaatt acctaagtcg gttccattga
      661 tcgataagtc tgtcatatca gaaaaataat ccggatcttt ttcaaagtta tcgaacgtta
      721 ctcctgcctt tcgccataaa gcaatttctg aatgagcgat cttcaaagaa gaatcgccat
      781 ttccttgaaa tacccagcca cctatgccat tagtctcgct tccgatccgt ttgttttgga
      841 aatcgaaata ggtaggagat tctaagtcta cattcaacac atccccactg atgatgccag
      901 tcttatcttc cccagttttc ccgaccatga ctaaataagt tttttcgcca gccataaagt
      961 tcaaatcaat atctccagtt gtatctacgg tagctccgga atctgcgcgt atatcgatat
     1021 ttgatttttc tccagtaacc gtaaaatcag ctgaccctgg actttctaga aaaccagaag
     1081 cgtcataaaa ttggatcgct tggtttcctt gattgagtct gttcacataa gatttgccat
     1141 cccctgcatt tcttaccttc aatgagcctc cattagttac tgttagtttt tgcgtaccgt
     1201 tctcaaaacg aactgcgcta gaacgaccac tttgcttatc aatatcgatg actgatccgg
     1261 ctccttctac gatcaaagaa agtcctttcc ctacaaaacg catcccattg tttccaggtt
     1321 ggtcgccatc tccagccatt ttgaccgaga tttgtccacc acttgataca tggatttgac
     1381 tagaatctcc ttgcaggcga aatccgccag tagtcgctgt attgattgtg attttcccat
     1441 cattcgacac agcgactctt gatcctgagc ctctcatttg gataattccg cgattgttgt
     1501 cgttggtaat attgatcttt gtatttaccg aaaattcact tcctgagcca ctgactttaa
     1561 atgtgacatt cccgggtagc gtatcgacag cctctccttc atcgctgacg ccatctgccc
     1621 gatcatagtc aagagagacc tttgaaccac tcaagatatg gagtcctggt gctgtttttc
     1681 cactgcccgt ctcatcacct gaatctagaa aaccatagaa ttttcctaga tatggtccat
     1741 aaagctgact accattgatt acatttagct gatagcttct acaaaaataa gctttacccg
     1801 gagcggcatt catcccttct ttcactggtt ggacatccaa tacaccggaa acaccatcta
     1861 atgtgacagt tgcacgaggg gcgtaaatca ggcctaattt agaaccatca tataagtcaa
     1921 gtgtcccggt aaattttatt tctcccgtaa aatgatatct gtttccccaa aaatcggaca
     1981 tataagcaat gataatggca tttccatcga tatttggtat caacccacct ggatgagctg
     2041 ccgttccttt aaaattctga aactcgatcg actgaggtgt ctcattgata tagatgtgat
     2101 gtgtgccgaa attatgagca agtccgccaa aatcgataac gatatctcgc ttgatctcca
     2161 attccctgtc taaagtcaaa ggctgagtta aacgaatctt agctactgat tcgtctgcga
     2221 gaagctgcgt cagttcttct gttgtggcag cttcgctgta cccatccgct gaaacaactc
     2281 taacctgtac ctcttcactg ctatcagacg tttgttcagc tacttctgag gtctcacttg
     2341 tcttattttc ttcttgcctt gtattagaag taggcgcctc actttgggat tctgtagttt
     2401 cagaagtaat tgattcttct gaaaaagtag tcttgtcaaa actttgattc tctgattgcg
     2461 ttgattcgct tgttttctct acatcggcaa aaacggatgt caccatcaag agagggaaga
     2521 caaacaaaat cgacgcaatc agctttttta catattgatt cattctccac catcctctct
     2581 gacgttttga caattttatt ttaacgtttt aatttatttg taattgtact ttcatattta
     2641 aaatatgtat ttttatggca aatttttctg ttaggtgaaa atttcttttt tgtacaaaaa
     2701 aaggctgtga caaaagtctt gtcacagtcc cttattccga acaaccgatg tcctcctgac
     2761 aactcttcgg tcacaacctc cattttaaca ataaacctgt aaatgtgagg gtaacacttt
     2821 gattgtcaca ggtaatttat ccccttcatc tccatcaaca ttcgtcgtca attcttcctc
     2881 atcagctaat gacacaacta attgctcaca agtaagatag gctagatttt tcgtagattc
     2941 atctactcct tttaataggt ctggcaatgt tttaagcgta tcgatcaaag aagaatcttt
     3001 taaataaacc aaatggagct taccatcatc cacttttgca tttggaagta atgtttcaaa
     3061 accgcctact gagttagtta gtcctatcag caagatactg ctctcaattt cttccttctt
     3121 tccatcaact tctacgtgaa acgagtagga ttgtgtggaa gctaactgtt tgattcctga
     3181 catgaaataa gctagttttc caaatttcgt cttcttctcc gcatctacat cattgattgc
     3241 ttcagggata gagccaattg cgacgacatt cataaaatag tcgtcattta tttttccaat
     3301 atccaatggc ttgactgatt caatcgaaaa atgattgatc gcttcctcag gttctagcgg
     3361 tatccctaat gctcgagcca agtcattcac cgtacccaat gggaaaaatc cgaaatttgg
     3421 ccgatgttct tgttcagcga ttccactgat tccttcgttg actgttccat ctccgcccat
     3481 gacaaacaca ctatgatacc cttccgtagc cgcttcgcga gtaaagtttt ttgcatcccc
     3541 tgcttttttt gtatgcaaga cgaccacttc atcaaacacc gattccaatt ttgctatcgc
     3601 taattgctca aattcctttg cttgttcacc gcctgaactg gggtttacca ctaataaaac
     3661 ttttttcatc actttctttc ccttctgatt gatttcttta atataacgat agcataaaga
     3721 aaaaaattag gctgtgatga agtactgttc atgaaacatt catttatttt ttgacaagat
     3781 aaaataccca tactatgaaa ctgaggaaaa ttaaaacaat gatataggaa ataaaagaaa
     3841 atgataggca ttgtatacct aataaagtaa aaatcttagg tggcagataa aaacacaaaa
     3901 atagcaagag aatgtaatag cgtttcttcc actttttgtt atcagacctt atagcgatca
     3961 gaggaatacc aaatgcataa ctgaaaaact cttgatgtac tgttaatatg tcacgacctt
     4021 gaataataaa tgcaacaatc cccgctataa ttagtgtgcc taataccaaa acggaaaatt
     4081 gatgaatata ttttatatac aaatgatcca ttgtacttcc tgccttttta tttgtgagaa
     4141 aaacaaatcc gtatacgtga ataacttcta gaaataaaag aaatttgcaa aaccactcca
     4201 tttatctact tctacgtgca ttttttaata tcgttttctt tatttaagat tatacagaaa
     4261 aatatctagg gtggctggat tctaagtaaa aaaaattttt aaaataaaaa gaactaaatc
     4321 aagtaataat taaactactg ttgatttagt tctaataaaa agatttagat gaacagtttt
     4381 attgatttaa atagttcata ttttttattt ataccgctta tttactactt gctgtactag
     4441 aatctgaagt gaatgtactc aaaacatttt caaagtcttt gtctttgatt tttacattag
     4501 cgtctttcaa tacttcgcca attactttat tctggaaggt actgtcactt aattttgtgt
     4561 ctgttgcaat ttctttcaat tcttttttgt atttatccat gtcgttgcct ttgttttggt
     4621 ttttcaccat tttaaccaca taatattccg tagtataagt tgaagcattg gtagaagtga
     4681 tcacatcaga aacttgacca tctttcaact taaaggctgc ttctttgact tctgccggaa
     4741 ccgttgtagt agtggaatca aatttcactt ttccgccatc ttcttttgtc gtagtatcag
     4801 ttgacttgtc tttggctaat ttagagaaat cgtctccctt atcagcagct ttcttcacat
     4861 cttttgcatc atcttcactt gctacttgga taatttgagc ttccacttct ggatggaaag
     4921 attcccaagc cgtttttaaa tcttcatcgg tcaatttgat atgtttcttt agaccttctt
     4981 gaaaggctag actttgtttg atactgtctt tgaatgtttg ttctgtgtaa cctgcagatt
     5041 tcaattgtga atcaaatgaa tcacccaatt gtttctttgt ttgatcatat tgggaatcga
     5101 tttctttggt tgatacgtca tctccatatt tatcttcaaa tacttgataa acgatcaact
     5161 gttgcacagc ggaatctgct ccttgcattt ttttcaagct atccattact tcttgttcgc
     5221 tgattgtacc gcctttataa ctaacggctg ttttttcacc ggcattcgta cagcctgaca
     5281 ataagccagt gcctagtacg atggcaagtg ctaaaaatct ttttttcatt tatcaattct
     5341 cctacttttc gttttttgcg aattgctaaa aacaaaggtg ttcaagatga aaatcatcaa
     5401 gaacaccttt actaatctgc tcgatacgtc ctgaatagat ataaggctac acaatccaga
     5461 aagttcgctc agcagaataa atgtcaagta gcacatcaca ccttaatata ctacttcttt
     5521 tgttaccgtt tttcaagtct atcttgcgtt ccgtatttaa cgtaactaat tcttagaaca
     5581 aatgttatcg attttgtttt tcttcttccg ctgttgggat attcaaatca gacactttta
     5641 tgttcgattt tccagacatt tttttgttgt ttttattaga aaaagtttgt tttttcattt
     5701 gttttctaaa gcatgatctt cttgtttcct attttggtca gatatggatc atgcgtgacc
     5761 acgataacta acgtaccttg ggaagcctgt tgcgataaga tttcaacgac ttgctcctta
     5821 tttcgtttat ctaatgatcc agttggttca tcggctaaaa tgattttgct attcttcaat
     5881 aagactctcg cacaggcgat tctttgcttc tcaccaccag ataatgtgta gataggcgta
     5941 cttagcttct tgtttatatt tacttttttc aatgcctcat actttgctgt tcgcttatct
     6001 gaaacattta aattcgaaaa cttcaatcca atatctaaat taaaatcgat tgtttcatat
     6061 tcaatcaacc caaaatcttg aaaaagaaaa gagagggcat ttctttttat caagcgcttc
     6121 tctcttccag ttaaatgttt tgtttcttga ttaaataaat aaaattgccc tgaactgaat
     6181 tgatcattca atcctaatat attcaataaa gtacttttcc ctgctccgct ctctccaact
     6241 acagtcacaa aatccccagc atccgcagtg aaacgatcca aatgaagaat cgttttgttt
     6301 cgaatgattt ttttcgcacc ttttatcact atcaacggct ccaattgtta acctccattc
     6361 atcagcaata actgactttt cttttctgta gacaaaataa aaacaactga aagaataaaa
     6421 tcaatgaagc taaagaaaaa aatattaaaa agatatttag gctctctttc tataagaaaa
     6481 aggaagatta cacttgtcgt tccccatttg atgaggttca tcatgaagaa atctttatac
     6541 gtttcaaaga gcgaataacc acttgaacga aagatagcga ttttccgaca attgtagaca
     6601 aaatatatgc ataacatgac atagctcaac aagaagaaag aaaagaaagt gagtaaaaat
     6661 atctgtatca agtaatgaaa tgatctgctt aacactttga cagctatctc ttcagtctga
     6721 gtatacggaa atgataactg caagttatca cgtaactgac tttcttcaat cagctcaaat
     6781 aacctttctt tcgacaaaga gcctatcttg atctttaacg gtggatagat ttctccgctg
     6841 agaatatttc tttcccaata cgtactgttt ttcaatgtca gtatttcaac aaccggataa
     6901 tgttcgatcc aggggataga aggaataaat gtataaatgg gttgattgtt ttttataaat
     6961 agaaatttcg gttctttttc actgatttct tgataatatt ctgttatttg cgccaggtca
     7021 ttttttaaat ctgtatgtct atatcgttca gggatcaata taacagggtg cgtttctttt
     7081 tcggttattt ggatcaacct tccagagaca tcaatcaaag gaaatttctt aagataattc
     7141 agatttattt ttatccctct gccaaactgt tcattttctt cattcaagta atctgataca
     7201 ttgactaata tacttccgtt tttattcaaa gtttcatata attcctgatc ggctgctgat
     7261 gcaaaacgat tggaataaat aaaatccacg tggtttttcc cgatggttac tggataaaat
     7321 acatgatagt cctcaccagc gatttctttt ggtgtttcat gggaaacagc taactgtgaa
     7381 gaagaaaaaa cgatagttgc taattcgaca ttggcaacgg tcaaaagaag agaaaacaac
     7441 aatccaaatg taaaaagaat gtattggata tagttgaacg cttctcttaa cgaagcgatt
     7501 ttttcaacta ataaaagaga aatcaaagag ataccctata ttccgctgaa caagaaaacg
     7561 tacatctgga acaaataaag aaattgctcc ttataataca taaaaataaa accaccaatg
     7621 attcctgcaa ttatgaatgt aataatgatt ttctttttcc ccaataaaca ccacaatact
     7681 tgatgtaatg aaaagccatt attcaacaat aaactgattt ctttattcat caataagaaa
     7741 aaggatgata acaaaaggaa taaaagaatc aatccaatag ttaaatacac tcgataatta
     7801 tatacgtctg tacttgaaat aaccgaatac gaatagctat tttcaaagtc ttcaaaaata
     7861 ttagtggcat aatcagtgtg aaatgcttga ttgaatcgtt ccacaaatag taaaatgaac
     7921 cgttcatagg cttctttttc tctcgtcatc aagaaaaatt caccttcaaa atcttttatt
     7981 ttgatcgatt cttcaatgga cgttacgcga aaggaggtat tagaataggg taccttctcg
     8041 tgagaagatt tgacatcccc ttgataatac aatgtcattt caagctttgg ctgtatcttg
     8101 aagttccaag atgtatctac ttcattggat tcatttactg tttttttcat ataagaaagc
     8161 tcagtttctg atgagacatt ttcaaatacc atgattagct gctcatattc ttcttgccca
     8221 ataaaatgtt gtggcaatcg aatcggagta taagtatctg tttgatggaa aaattcattt
     8281 gtccgataga gtacctgttt atttataata accaaggcaa aaccactcat gataccggtc
     8341 actatgattg tgagtaaaac tgtgaacttt tttttgatta tcaccactct cccttactta
     8401 aatgattctt tgataaaatc ataccgtttt tcatcaaaaa aacgtatcta tcaaatgtat
     8461 aggtcatgta gaataaatct atatactgta gaatcgaaag gagaagaaga tgaacatcgg
     8521 agaaaaaata cgaacttacc gcagaaattg taagcttacg caaaaagaat taggcgaaaa
     8581 aatcggtgtt tccgataaaa caatctctag ttgggaaaat tctcgtacaa tgccagacct
     8641 tgaaatgctg tcacttttac atcagacttt aggtctgccg attgattttc cttctattga
     8701 atcagctacc gaatcaatag aaccaacatt gccggatgaa cctcttgtta tcaagaaatc
     8761 ttattcatca aaacaaccct tctcactcaa aaagacacta tctttctttc tgctcgtctt
     8821 ttgtttggtc tatctttttc ttatcaatta tcaatataaa aatgaaacgg ttgatcgatt
     8881 caatcctttt ttgaaaccac aaatcggcta taccacactc cctgtaggat acgtggaaaa
     8941 ttccatcgaa cattgggtat gtaatgatat ctttgttcat ggtacatggc tgtcttttca
     9001 cgctggagag gtagaaaaag gtaagaatca tgctgtaatc aaacacaaag gaaaacacgt
     9061 catgaacgtt cgcctgctca cttgggaagc cattccctca gattaccaag aaatcctcat
     9121 ccaagactat gacgatgcct taaaaagaag ccctcaggag ccagataggc attaagtagg
     9181 agatgaacgt agagatagaa aaaatcaatg cacatgatct tatcaaaaaa aaacaggggt
     9241 acaaattaat tgaaaaagct ttgagatgaa agttcctaaa taaaaaagtt cagaatgata
     9301 ggtaaaaaaa tgactaacta ttctagtatt aggctatttg tattattctt ctgttatttt
     9361 tatatcaaaa aatccagaaa taactaaata taggtatttc tggcttatag tataatattt
     9421 acgccacagc ccccaatgat tctttttgga atcatgatca aggacggaca tacacaaaaa
     9481 tattttgccc attcgatccg aatagattaa cgactaatct agtaagtaat atagattggt
     9541 acgtagtcaa accagcaaat ggaaaagttg tctattccac cccaagggct ttaaagacag
     9601 catcttccgg cgtttgataa aaaatcaaat taaaggtacc cattaattct gggggtacag
     9661 ttccaatatc agcagcgctt atcataggta caagtacttt tttcgctccg gcatcttttg
     9721 ctacttgtaa acagttagct aaattatcta ccttaatcat agaaccacca attgtaatat
     9781 cccctaaaat aactaaactt ggtaatacag gtttatttaa ggaaactgag cataaagcta
     9841 ttatagttga taacgataat gtttttgtca taccaatgcc attcatatct tgaacatgaa
     9901 cgacaaaatc ttttgtcgtc aaactaatag actgactaac acttccataa ttagctctta
     9961 aatatcggta tgccgaatcc atcgcttctc ttgctccagc atctgaattg atcccagtac
    10021 gttcaaactt tccatttccc gaagtcattt gcgtttccaa tttataggta cctaattgcc
    10081 cagttttgcc ataagatacc gtgtacaaat gccctggctt ccctaaccct tctggaatca
    10141 aagttcctcc accttgttca ggaacagaaa catactcttc tcgctcttct tctaaatcaa
    10201 tgtatgaaaa attgacatca taaaattcca ttccaccaat tttctttaat tgttctttta
    10261 cacgacgacg catttcaaga gcaaatctta gtatttcttc gacatcactt ttttcaaatc
    10321 taccatcagg atacaacagt ttcatcatcc cagatacaat ccgacgaact gcaatcgtat
    10381 cccgctgatt taattgagaa ccaagtttga aatatttatc aaaactatcg ccaaaactaa
    10441 cttttcgaag ctcacgcatg acttcagaaa aataatctgt aatgaagcca tagtcatttg
    10501 taaaaaaatc tggtcgatat ttaggaattt cccaacctgg taaataacaa tgcatccgat
    10561 ccaaaaaggc tgaatccgtc cccataacct ccggaaatgg ctcaaataaa ttcgaagttt
    10621 tcaatattac atcaacgctt tgattaatat ttcctacaaa agccattgat gctgaagcat
    10681 tcttttcttc tttgcctcta gcaaaagatc ctgaagccat gtaatctttc atgatttgaa
    10741 cgccatcatt atccttaaat ttaatcccag caacttcgtc aaaagcaaca cagtcccaca
    10801 tacctacaag tcccattgta cgactagcca tattatagaa aaggtttgct actgttgttt
    10861 gtccacctga aacaaggatt gagtttggtg aaatttcttt atatacatgc gatttacctg
    10921 taccacgtgg tcctagttca cagagattaa aattattttc aattaatgga ataagtcttg
    10981 ctaaatgaag ccattttacc cttttattaa attgtgtagg ttccatacca atggaccgta
    11041 gaagtaagtc aatccattca tttgtagtaa actccttgcg agccatcttg taagcctcaa
    11101 tgtctaaacc aggcatttgg attggatcaa ccttttttgc aataaaaggg ctattccaac
    11161 gatcattctc atcataccaa tattccatcg aaacaataca ccagatcccc ccacctaata
    11221 atcgatcaaa ttttttcgga tactcttcac taatttgaac attttttaat cccaggttag
    11281 agaattctgc ttcataacaa tctttctttt catttaaatg tacagttact ttatcaataa
    11341 ctgtataacc acccaattca cgaatttttg cttttacttt ttctgcttca tcaggacgca
    11401 catagtttcg agcaagaata tttttaacat tttccacacc tttttcaacc aatgtttcat
    11461 ccaatgacga agcatacata ccaagtaaat attccaatac ataaacaggt acattcgcac
    11521 cttctttaat tttctttgtt aaatcttttc tgacaatctt tccgggaaag aattcagata
    11581 actttaaaat ttcctgttgt tccactagaa ttcctccatg tgttcagtca ttataatagc
    11641 aattaaa
//
LOCUS       DAZKLA010000090        11631 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000090 DAZKLA010000000
VERSION     DAZKLA010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.091"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            237..1877
                     /locus_tag="ACG85N_002056"
     CDS             237..1877
                     /locus_tag="ACG85N_002056"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFQ0224789.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="ACG85N_002057"
     CDS             2031..3353
                     /locus_tag="ACG85N_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFQ0224790.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="ACG85N_002058"
     CDS             complement(3415..3846)
                     /locus_tag="ACG85N_002058"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0224791.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="ACG85N_002059"
     CDS             complement(3910..4746)
                     /locus_tag="ACG85N_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFQ0224792.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="ACG85N_002060"
     CDS             complement(4936..5238)
                     /locus_tag="ACG85N_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFQ0224793.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="ACG85N_002061"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="ACG85N_002061"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFQ0224794.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="ACG85N_002062"
     CDS             complement(5692..5958)
                     /locus_tag="ACG85N_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFQ0224795.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="ACG85N_002063"
     CDS             complement(6212..6937)
                     /locus_tag="ACG85N_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFQ0224796.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="ACG85N_002064"
     tRNA            complement(7303..7373)
                     /locus_tag="ACG85N_002064"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="ACG85N_002065"
     CDS             7490..8242
                     /locus_tag="ACG85N_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0224797.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="ACG85N_002066"
     CDS             8258..8737
                     /locus_tag="ACG85N_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0224798.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="ACG85N_002067"
     CDS             complement(8988..10568)
                     /locus_tag="ACG85N_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFQ0224799.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="ACG85N_002068"
     CDS             complement(10561..11304)
                     /locus_tag="ACG85N_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224800.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..>11631)
                     /locus_tag="ACG85N_002069"
     CDS             complement(11480..>11631)
                     /locus_tag="ACG85N_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFQ0224801.1"
                     /translation="YIINTENITSISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKI
                     ENESF"
BASE COUNT         3535 a         2317 c         2064 g         3715 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa g
//
LOCUS       DAZKLA010000091        11570 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000091 DAZKLA010000000
VERSION     DAZKLA010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.092"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            104..1075
                     /locus_tag="ACG85N_002070"
     CDS             104..1075
                     /locus_tag="ACG85N_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFQ0224802.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(1052..1141)
                     /locus_tag="ACG85N_002071"
                     /pseudo
     CDS             complement(1052..1141)
                     /locus_tag="ACG85N_002071"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(1153..1389)
                     /locus_tag="ACG85N_002072"
     CDS             complement(1153..1389)
                     /locus_tag="ACG85N_002072"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0224803.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(1358..1489)
                     /locus_tag="ACG85N_002073"
     CDS             complement(1358..1489)
                     /locus_tag="ACG85N_002073"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0224804.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(1565..1948)
                     /locus_tag="ACG85N_002074"
     CDS             complement(1565..1948)
                     /locus_tag="ACG85N_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0224805.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(1981..2766)
                     /locus_tag="ACG85N_002075"
     CDS             complement(1981..2766)
                     /locus_tag="ACG85N_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0224806.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            2944..4044
                     /locus_tag="ACG85N_002076"
     CDS             2944..4044
                     /locus_tag="ACG85N_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0224807.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4034..4858
                     /locus_tag="ACG85N_002077"
     CDS             4034..4858
                     /locus_tag="ACG85N_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFQ0224808.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            4855..5670
                     /locus_tag="ACG85N_002078"
     CDS             4855..5670
                     /locus_tag="ACG85N_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0224809.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            5667..6497
                     /locus_tag="ACG85N_002079"
     CDS             5667..6497
                     /locus_tag="ACG85N_002079"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0224810.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            6504..7535
                     /locus_tag="ACG85N_002080"
     CDS             6504..7535
                     /locus_tag="ACG85N_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224811.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(7642..8915)
                     /locus_tag="ACG85N_002081"
                     /pseudo
     CDS             complement(7642..8915)
                     /locus_tag="ACG85N_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            9142..9327
                     /locus_tag="ACG85N_002082"
     CDS             9142..9327
                     /locus_tag="ACG85N_002082"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFQ0224812.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            9352..10071
                     /locus_tag="ACG85N_002083"
                     /pseudo
     CDS             9352..10071
                     /locus_tag="ACG85N_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(10069..10589)
                     /locus_tag="ACG85N_002084"
                     /pseudo
     CDS             complement(10069..10589)
                     /locus_tag="ACG85N_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            10877..11530
                     /locus_tag="ACG85N_002085"
     CDS             10877..11530
                     /locus_tag="ACG85N_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0224813.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT         3326 a         2147 c         2655 g         3442 t
ORIGIN      
        1 tattttacat cctagtatat ttatatgagc taattttatg attaaatggt aagtttttcc
       61 ttgtgtgttt tagtttaaac tagtgtgtaa ttcaaaaatc tatatggaag gaacagtatt
      121 aagaatagaa cagggttctc tacatgatgg tgctggttta cggaccgttg tttatttaaa
      181 gggctgccca ttacgctgtg cctggtgttc gattcccgaa tcacaaagca agcagattga
      241 aaaaggattt ggccagacca tgaccgctga agaggtaatg gatgagattg agaaggatgc
      301 tgttttttac tttcactcgg atggcggagt gaccattagc ggaggcgagg cactggttca
      361 ggcggatttt gctaaagaaa ttttgcaaaa atcgaaatac attggaatca ataccgtact
      421 ggaaaccagc ttttgtggtg cttataatga aatccaaaag gtagctccct atgtggatac
      481 attgtttgtg gatgtaaaaa tgtttaccag caagctccac aaacaatgga ccggcttaga
      541 taaccaacaa attttgaaga atattcgtcg ctttttaatt gagtatccta actgcgaagt
      601 gcgaattcga gttcctgtgg ttccaggtat caatatgaac ctgaccgagc tgctgacgat
      661 tgcttgtttt gtggcagatc ttgatcggtt cgtaccgtta gaattactac cgtaccactg
      721 ctatggtatg cacggttacc aagcattggg actggagtat ccgttggcag acacgccagc
      781 gccttcacct aaagaaatgt ttgcattggc agatcaactg gctcgcacgg ttcctcattt
      841 gctggtcaca gctttaacca aaacatttat ttatttattg aatccaagct ttatcttatg
      901 gataaatgaa agacactcac tagctgcagc cagctcaaga atagagcttt tgctgaagtc
      961 agtgagtgtc tttttacgat gttttttcga agttaatttg tcgaatgaac actggctcaa
     1021 tcggtacatc ttcactgttt gtcggctgag ctgcgatcgc tgctacgatt tctaaccctt
     1081 ggtatacctg tccaaaaaca gtataacgac catccagctc cggtagtcct ccaagctcca
     1141 agtatgcttg ctttacatta gcaggtcagc ctgcgccttt gtgttttgca caataaaaaa
     1201 ctggttgccg ttggtgtggg ggccgtggtc ccctaaggcc aaggcgccat taaaatgata
     1261 aagtccgcga tgcacttcat cagcgaaaaa gccctcatag ctggaaacag cttcatcacc
     1321 agaaccatcc aaagcgccac cttgaataac aaattcctta atgacacgag ccgaaaggtg
     1381 tgttgtcata aacgccttgc tcagctaggg agcgccagtt ggctacagcc aggggagctt
     1441 ggtcaggaaa taagcggaga cgaatttcac cagcatcggt gaccagcaca caaacttctt
     1501 cctgctgcgg tttttctaat tgaaagagag gaaggtttgc aactgaaacg ccttgatcct
     1561 gccatcaaat atcgaaaatc cgccactcga ccttcgcctg atcaccaagg cgagccattc
     1621 ccaataaaac ggtataggcg gtttcatccg cttgaaaggt caattgcagc tgtcctcgta
     1681 aatcgttgta gtcatctggg gcggctactt gataaaaaag atgaaactgc gcatgcaaat
     1741 attccaaagg ctgatcttta aaggctgcaa aataagcctg aactttttcc cgctgcaagc
     1801 tgtcagccac caaaaaattt tccagttgtt gttttggaac ggctgctaac aaggatcggc
     1861 aaaccgcctc aattttcaac gcatcccaat gtcgcgcaat taaataatgg atgggtgcct
     1921 cttgattgta gtcccacagg ttgctcattt ttttcaccac tttcttctat tatataggaa
     1981 tcacaaaatc ggcagcggcg tcaggcggcg ttcttttcct tgaatgacta gcaacttttc
     2041 tacctgacag tctgccaaca gctgactcat ttttttaaag cccagctgat aatcttcggc
     2101 aacatgagca tccgagccta aggtaaagag ctttccgccg acactttgat acaaaggaac
     2161 ggcataacgg taaaggtctt cgttgccgta tttcaagaag ctcttggcat ttaactccat
     2221 cgccaattct cgttcaacaa cctttttgaa aatcgttagc agctgagcct caaaatgctc
     2281 cgcgaattct tccgctgtga aggtgaagcg gcgtaatcca taatcaaagt gagtcaaaat
     2341 atctccttct ggaaaatccg tcaagacctg caacatctga tcaaaatatt ccttggtagt
     2401 ttgaatggga tctttggtta aaaccacatc gtccatataa tcaaagcggc cattttgatg
     2461 aatacttacc agctttaaat cataaggatg ctgagcgagg taatccacga tccgctcttc
     2521 ttgtcctgga acaacgccaa tctcgatacc tcgcagaaat ttggtggaat aggtttcttt
     2581 taaagagtcc agtttgttta tataggctgg ataatccgga atgtcatctt ttccggtaga
     2641 tggattgttt aaatcaaagt gatccgtaga aacgaaaaac tccggctgat aggctaaata
     2701 gttttcaaat ttttcctctg aatcaaagga aaaatacgtg tggagatgct gatcataata
     2761 ctgcataggg agcctccttt tttctactat cataccgcaa aagctttaga aggttaacgg
     2821 gaattcgtcg aaatttacaa accatataca acctttacat tgttcatgaa accttttcaa
     2881 gaaactgatt ctctcattac aaagctttgt tagaatggaa ttatcgaaag gggagaagaa
     2941 tgaatgaaaa tgaatgaaaa aattcgtaac atgactatcg gtttattttg tgcagcaaca
     3001 gtaagtatcc tgctgacagg ctgcggctca ggtgaagcaa cgaagcaatc ggctgcggca
     3061 gacgactctt tagatacgat tatagaaaaa gccaaggaag aaggagagat tgccagcttg
     3121 ggaatgccgg atacttgggc aaattgggta ggcacttgga acgaccttga aaaagaatac
     3181 ggcttaagtc acgtggatac ggatatgtcc agtgcagaag aattggctaa gtttgaatca
     3241 gaaggaaaaa atggaaccgc cgatattggt gatgtcggta tcagcttcgg tccgttggct
     3301 gaaagcaaag agttaacctt accttataag accaactatt gggaggaaat tccggattgg
     3361 gcaaaggatg atgatgggga ttggttgtta agctatacgg gaaccatttc ctttatcacc
     3421 gataaagaga atgtgaagca agcaccgact agctggaagg agctgcaaga gggtgactat
     3481 caagttagcg tgggggatgt gacgattgcc aaccaagcac aatttgctgt attggctgcg
     3541 gcgattgcca acggaggtga cgaaaaggat attcagccag gtctgaatta ctacaaaaaa
     3601 atggcagaaa aaggccgttt gtccaccgtt gatgccagcg tagcaaatct tgaaaaaggt
     3661 gaaatcgatg tggcagttgt ttgggacttc aatggactaa attatcgtga ccaaattgac
     3721 tccgagcgtt ttgaggtaac tgtgccaaca gacggctcca tcattagcgg gtatacaacc
     3781 ttaatcaata aaaatgcccc tcatcccaat gcggcaaagc tagcacggga atatatttta
     3841 tctgatgaag ggcaaatcaa tttggcaaag ggctatgcgc gacctattcg caaggtagag
     3901 ctgcctaaag aagtagctga aaaattactg ccggaggatg cttatgaagc ggcggtgcca
     3961 gttgaagaca atgaagcttg gaacaagacc tctgtagagc ttccagagct ttggcaggcg
     4021 gaggtactga cctatgcaaa gtagacttga caaactgatt gtagttatac tagacggctg
     4081 tcgctacgac acagccgtcg cccagcttgg gttcatgaat catttagttg agcaccagca
     4141 agcaagctta ttgaaggtaa aaagtgagct gcccagcaat tcacgtccgt tatacgaagt
     4201 actgatgacc ggcgttccag cctatgaaaa tggcatctat agtaattacc atgctcagcg
     4261 atccaaagag tggtcgctgt ttgaattagt taaggaagct ggcggaaaaa caggagccgc
     4321 cgcttaccac tggtacagcg agttgtacaa tcaagccccc tataatcgtt tggttgatcg
     4381 catccagcat caggaggagc gactgattca gcatggcatc ttttatagtg aggacactta
     4441 ccctgattcg catttatttg cggatgccca ttatttagtg aatcagtatc agccagactt
     4501 tttggtcgta cattcgatga atattgatga tgtcggtcac aaatttaccg ccgattctca
     4561 tgaatatgca gcagcggtta atcaggcgga cagattattg gctatttacc ttcctgaatg
     4621 gctgcaaaaa ggttatcaag tcgtggtgac tgcggatcac gggatggatg cgcacggact
     4681 gcatggcggc agcttggctg cacatcgtga agttcctttt tatttgattt caaataaacg
     4741 ccaaaagctg aaggaacaaa ctgtaccaca gctgctggcg gctccattgt tttgctggtt
     4801 attaggaatt gcaccgtcag agaaaatgca ggacattcaa gaattgatga ggggatgaaa
     4861 gagatgaaaa aacgcttggt tctggttcct gcctccttta tcttctgtgc atttttactg
     4921 ctgccgctgc tttttatggt tctgtgcagc ttgcacagtg atttaactaa ccagtggaca
     4981 ttggccaatt acagtgatat tttcagcaac aaatattaca cacaggcctt tgttaacagc
     5041 gtactgattg ctgtcagctc cagtgtgatt gggttaatcg ggacgtttat tttatgttta
     5101 tgcttaatga atctatcgac taaattgcag gagaaaatca cctttgtttc caatttggca
     5161 gctaattttg ctgggattcc gctggcattc tcttttatta ttttgttggg gaatgtaggt
     5221 gtgctgaaaa tgattttgcc atttctagcg gacttcaatt tgtattcttg gtggggattg
     5281 accatcacgt atacctattt ccaaattccg ttaggggtct tgtttttgta tcccagcatg
     5341 aaggaagtga aaaaggaatg gctggagtcc gcacaattgc tgggcgcttc atcagcgtat
     5401 gcttggttca aggtggcttt gccttttttg cgaccatcga ttgccagcac ctttgtcatc
     5461 ttatttgcta atggcatggg gacctatgaa acagcgtatg ccctgaccag cagtaatgta
     5521 aatcttttaa cgattcgaat tgcggcttta gtgtcgggag atgtgttcgc caagcctaat
     5581 ttaggcagtg cgttggcagt cgctttaggt gtcctgctgg taacggcaat gttgattatt
     5641 caaaagaaag gcaaggtggt gcagacgtga aaaagttaat aaaaccgatt ttgttctgct
     5701 ttgtcctgta ttacttgctg ccgattattg gaacgatttt gtatgccagc tccactaaat
     5761 ggtcaaaatc gcttttacct agtgatttta ccttgcaatg gttccaacag ctgctgacgg
     5821 acagggaatt tattgctgcg gtgggtcgct cacttttatt agcaggtgtg gtcttggtga
     5881 cgattctgtt gttgatgatt cccaccatta tttggattca tttgtatttc cctcgtttga
     5941 atcgttggct ggaaaagctg ctcctgctgc cgtatgcttt accaggagtg attttggtaa
     6001 cggctttgct tcgaacgtat gctgaaactg gtatcccaat gtttgtagtg ttggtaggtg
     6061 cattgtttat tacggcgttg ccgattgtct atcttagtct taacaaccag atgcgcttaa
     6121 ttaatctgaa ggaattagtg gatgcagcag aaaccttggg cgccccgatg tctacgatta
     6181 ttatccaggt gctgtttcca aacatccgta tcggtgtgac cctagtttct ctgatgattt
     6241 tttcttcggt ttttggggag tacatgctga cgaacctgtt gattggcggt cgatttcaga
     6301 cggtacgcat ctatatgctg cggcggatga acgaaaacgg ccatttggct agcgctgtga
     6361 tggttttgta tttgctgttt atggtgggaa ttgctttaac tacctttatg ctgacttatc
     6421 gacaaaagca acagctgtcc cttaagcaga cgagggaaga gaagcagtcg aaaaagaagc
     6481 ccttaaagga gttgtgacgg aaaatgtttt tagaaataca tgatctcaat ctgttttttg
     6541 atgaaaaaca gattttagct attgatgaaa ttcagattga aaagggtgag ctggttactt
     6601 tactaggtcc cagcgggtgc ggaaagtcta cgttgctgcg ctgtatcacg ggattggaac
     6661 aaccaaagag cggtgagatt gtgctggaca atgagaatat tgccgataag cctacgaagg
     6721 atcgcaatat cggctttgtt tttcaacagt atgcattgtt tccaactatg actgtttttg
     6781 aaaatgtagc ctttggtttg aaggtaaaga agctgagtcc tgaggtgatt caggaaaagg
     6841 tttttgagat gctatcattg gtggatatga cggagcaggc agataaaaat gtccagtttc
     6901 tttctggcgg tcaacgacaa cgggtggcac tggctcgctc attggtcact gaaccgaaag
     6961 tcctgctgct ggatgagcca ttaagtgctt tggatgcccg aattagaaaa cagctgcagc
     7021 gggatttgcg cgctatccag caatcgctag ggatgacgat gatttttgtg actcatgacc
     7081 aagaagaggc gatgcgaatc agtgatcgga tttttgtgat ggaagctggg cgggtggctc
     7141 aggtttccac ctcgaaggaa ttgtatcaga atcctcagag ccgatttgtt gcggaattta
     7201 tcggcaacta taatcgtttc gaatggtatg agctgaatca gtatacccac gagttttccg
     7261 ctaagggcaa tcgctgcatt tattatttaa gaccggagct gattcaattt gaaccggttc
     7321 ctcagggggt taagattccg gttctatgga aggaatcatt tattttaggc aacattcaac
     7381 ggtatgtttt tcaaacgctg gagggcaaag aaattttagt ggatcggttg aacgatcggg
     7441 aagagaaaat tagcgatgcc ttatacattc agctgaaaga tattctagca atccctgtgg
     7501 aaattgatac tcagcatttt gcacaacaaa gctagtgtat agtcggcaaa catttctggc
     7561 tctttgtcaa ataggactga tagagtgata taagaacatc taaggtagaa ttgattttct
     7621 gctttagatg tttttttatg tttaataagt gatcagatga ttgattaaga aaattctggt
     7681 tctcatccta acccgtaagc tattcgtttc aatgctttga tgtgggtatg cagattttct
     7741 attttgccat ttgagtaagg caactctaag gctaaacgaa tgccggattc atagtttata
     7801 aacgcttttt ttatatgatg cagttctctt ggaaccgact tgggcattgt ttttacaagc
     7861 tgaaagaaga tgtccgcttc tttgtcataa aaagcttgaa gaatttcttg atagtaagca
     7921 taagctattc gtagttcatc agaagtagaa agtagacgtt ctacgacttc actttcggtt
     7981 aaataggccc aaccaaaatt acgccatttt gtccatttat cataaacaag cgtgtcctgt
     8041 cgtttgatca gaagtcgaga taaagccttc acttgtcgat agtgtttggc ttgtattgga
     8101 tcgtttcggt cgaagctttt catgactcat agtcaaatat ccttaaaact gcgaatgagg
     8161 tgcttgacga tgtgaaagcg atccacaacg agtttggcat gaggaaagca ctcttttatc
     8221 agcaaagcat aagaggcgtt catatctgtt acgagtattt ttacgtttaa acgtgcacgt
     8281 cgttcaaatt gcataaaata agaaatgaga tctttcttct tacgtgaagg aagaatatcc
     8341 agtaatttcc cagtatcgcc atccatacaa gaaaaagaat agggaccgac ttgatttttc
     8401 aatgaacgaa attcatctac caatgaacag cgaggcaacc aatttatttt cacgtttgtc
     8461 atggggagca gagaagcgag tgttcgcgaa acggttgacc aagaaacgcc gcaatgttta
     8521 gaaatcgtcg tcattgcttg tttttcagtc aattcctcca atattttata atgtacttgt
     8581 ttggcgataa aacagttttt ttcaacaatg taggtattcg ccgtaaaata ggtgtgacag
     8641 ccacgacaga ggtaccgttg ctttgccaaa gcaagataag tccgttgatt cgcacactta
     8701 ttgagaagga ccagcgattt tttctttccg ttcttaacga tcagcccatg ttgattcacc
     8761 tgtttacact gaggacaggg ctgaatcgtt ttaatgagcg tggcagaaac aacgatactg
     8821 ttgattgaat gtattttttt cttctcgaca tgagtgattt gaatgtttcc atctttgatt
     8881 cctagtaata gtttgataag atgaagatca ggcatacaga acgctccttt gattgtgttg
     8941 tggtgattca attctaacgg agcttctgta tgttttgttt ttttatgctc aaaaggactg
     9001 atagaatgat ttactatcag tcctagaaat tatagcgcca catttctttc acctttgact
     9061 tttcagacgg cagtttttat gctgatactg tagagagagc aggacacagc ttttgcttgt
     9121 tttgggtaag ggggaaagaa gatgaccaaa gcacttattt ttgattttga tgggacgatg
     9181 attgacagtg aatggatgtt ttacgagagt attcatcact atttactgaa gcctatttac
     9241 tggagcagtt cggctataaa attccttttg cagactatca acaaggtgtt gggacggcag
     9301 cagaactgtc cttttatgaa gggataagtc aagcggttgg gagaccagtg gatgctgaac
     9361 attttgagca gcggatcagc agcactggaa aatcggagta gtctcaattc tgaactgcgg
     9421 gagctagact atttttttga agcacatccg atgattaagg aaaaaattga ttcctttgtg
     9481 acgatcgatg atcttcaggt cggcaaacca gatccagaag gttatttgtt gtgcttgaaa
     9541 cggctgaggg ttgaacaggc tgttgtagtt gaggatagtc cagtcggtgc ccaagcagca
     9601 ccgcatatga cagttgatgt acaagcgttg gatgcagatt ttttcgcctt tagcgggcat
     9661 aaaatgtgcg ggccgacagg aatcggtgta ttatatggca aacgtcaatt gttagaacaa
     9721 atggagccag tcgaattcgg cggtgaaatg atagattttg tcaatcttta cgatagttca
     9781 tggaaagagc tgccatggaa atttgaagct ggtactccga acattgcagg agcaatcgat
     9841 tacttgaatg agattggtat ggagaatatt catcgttatg agcaagaact ggttgcctac
     9901 gttttaccta aattacaagc gatcggcggt atcaccattt acggaccgca agatcctgaa
     9961 catcatactg gcgtggttgc ttttaatttg gatggcatcc atccccgtga tgtggcgact
    10021 gctctggata tggaaggtgt cgctgtacgt tcaggacacc attgtgcaca acggttcatc
    10081 gacgatttct gtttgactaa ttggtcgttt aaacagtcga tgataatgcc gtttgagcgg
    10141 ctccaatgtc gtggaatctt tgagtaagag tttccaatat cgcttgagcc gacggtactt
    10201 cttctgattt tcagattacg tgattgattt tcgtacagtt ctatgagaat cgcctgcttc
    10261 agttcctttg ttagttgatg atgttcatca actaagtccg ttttcgcaga aaaggtctgc
    10321 ccacaatcat agcatagaaa acgggtacgt tttaattcta aaatagtcaa cttttctttg
    10381 actttcaaca tctgagtacg tgtcgtatag gaaccgttgc ggataatacg aatcgatcca
    10441 cagtgttcac aatgggaagg tgagtcatcc aatctccctt taataaactg cgcttgaacg
    10501 cctttatatt ctttttcaat cagccattct tcatcaaaaa ataaatgttt atctgttaat
    10561 ccgagtaatt ttctagtatg attatccata gagcgactcc tttggtaggg tgatgttttg
    10621 gtcgactaaa ttttaccaaa agtgagtctc tttttgagtt gaaacgaaga aaaagtgtta
    10681 gtagaaaatc gccttcgatt ttccaacaac acaaaatatt atagaagcct tttttctaaa
    10741 ggatttcaag gtgtctgttt gcatttatta aaaaaaagaa aaagtattgg caacagaagg
    10801 cgtcaccaac actagatatt atagagccat aatatgctgt ataatatcta gtgtataggc
    10861 ttaagtggag gtatatatga aaatattaac tgttgaagat gataatatga taagagaggg
    10921 aataagtgaa tatctttcag aatttggata tactgttatt caagctaaag atggaagaga
    10981 agccttgtca aaatttaata gcgatataaa tttggttatc ttagacattc agataccttt
    11041 tataaatggt ttagaagtgt tgaaagaaat tagaaagaaa agcaatttac caattctaat
    11101 cttgactgca tttagtgatg aagaatataa aattgatgca tttactaatt tagtagacgg
    11161 atatgtagaa aagccatttt cattgccggt cttaaaagct agaatagatt ctttaattaa
    11221 gaagaattat ggacatttag agaagtttga atataaggat ctatcggtta attttaatag
    11281 ctatacagct aaaataaatg gtgaagaaat agatgtaaat gcaaaagaac tagaagtatt
    11341 aaaatgttta ttggataata atggacaagt gttaacaaga atgcaaatta ttgattatgt
    11401 atggaaagat agcgaagaaa ctccttatga tcgagtaata gatgtatata taaaagaact
    11461 tagaaaaaaa ttacaattag actgtataac tactataaga aatgtaggat ataaattgga
    11521 gagaaaatga aaaaattaaa gatatttcca aaaatgttca tacaaatatt
//
LOCUS       DAZKLA010000092        11285 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000092 DAZKLA010000000
VERSION     DAZKLA010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.093"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(302..2311)
                     /gene="metG"
                     /locus_tag="ACG85N_002086"
     CDS             complement(302..2311)
                     /gene="metG"
                     /locus_tag="ACG85N_002086"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFQ0224814.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2579..2857
                     /locus_tag="ACG85N_002087"
     CDS             2579..2857
                     /locus_tag="ACG85N_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224815.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            2954..3775
                     /locus_tag="ACG85N_002088"
     CDS             2954..3775
                     /locus_tag="ACG85N_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0224816.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(4060..5070)
                     /locus_tag="ACG85N_002089"
     CDS             complement(4060..5070)
                     /locus_tag="ACG85N_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0224817.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(5082..6035)
                     /locus_tag="ACG85N_002090"
     CDS             complement(5082..6035)
                     /locus_tag="ACG85N_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224818.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(6025..7095)
                     /locus_tag="ACG85N_002091"
     CDS             complement(6025..7095)
                     /locus_tag="ACG85N_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224819.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(7101..8144)
                     /locus_tag="ACG85N_002092"
     CDS             complement(7101..8144)
                     /locus_tag="ACG85N_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0224820.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(8145..9086)
                     /gene="opp3b"
                     /locus_tag="ACG85N_002093"
     CDS             complement(8145..9086)
                     /gene="opp3b"
                     /locus_tag="ACG85N_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0224821.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            9170..9517
                     /locus_tag="ACG85N_002094"
     CDS             9170..9517
                     /locus_tag="ACG85N_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFQ0224822.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9587..11248)
                     /locus_tag="ACG85N_002095"
     CDS             complement(9587..11248)
                     /locus_tag="ACG85N_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0224823.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3173 a         2302 c         2057 g         3753 t
ORIGIN      
        1 tgcattcaaa ctttatgaat gtaaggacaa taaaaaagag taccagatga atcaattaaa
       61 atctgcccga ccctttatca ttatgctttt tttctgtttt tacgattcct attagtatta
      121 ttctaaatta acaaaaatca gcgaagctcg tacttctttt tctaggattt attttggata
      181 gttctctgtt tctgtattat atgattggct tcaagtaata agaaggaatt tacgaaaatt
      241 gcttctcaaa tttcggaaaa agggggtatg acacaactag tgtcataccc cctttgaatt
      301 tctatgcgat ttctgagcca tttggcatgg attttggtgc ttcgattact tgtaaagagc
      361 catctggtgc ttctgcagaa aggatcattc cttgactgat ttgtccacgc atctttcgtg
      421 gtttcagatt tgctacaatg acgacttttt tcccgatcaa ttcgcttgga tcaggataaa
      481 attcagcgat tcctgacaag atttgacggt cttggctgtc tccagcatct agtcggaatt
      541 gcagcaactt atctgcccct tctacttttt gacagttgat gacttctgct acttttagtt
      601 ctactttttc aaatacatca aatttgattt gtttttcttt cgttgaaaca agttctgttt
      661 cttctggatc ccatttgact gtatcttcgt ttgtttgagt accttcagac attttttctt
      721 gaataaaagc aacttcttct tccatatcta aacgtgggaa gattggtgta ccttttgcaa
      781 ctacttttgt tcctgaagga aattcgccaa agtgtaatcc ttcaagattc attgtttcag
      841 agtccaatcc tagttgattg aagatcttct ctggcgtttc tgtcatgatt ggctgaagta
      901 atatcgctgc gatacgcaaa gattcagcta aatggatcat gacactattc aattcagctt
      961 tcttttcttc gtcttttgct aatacccatg gttctgtttc atcaatgtat ttgtttgcac
     1021 gagaaatcag cacccagatt tcagccaatg ctgtattgaa ttccattttt tccattgcat
     1081 catggtaacg tccgacaacg ttagcagccg tcgtagacag ttcactatca aatggagtga
     1141 caagtgaggc atagtcagga acgagtccat cacaatattt gttgatcata gcaatcgttc
     1201 gattcaataa gttccctaga tcatttgcca gatcataatt gacacgggaa acaaaatctt
     1261 ctggcgtaaa gacaccatct gacccaaacg gaacagcacg aagcaaataa taacggagtg
     1321 catctaaacc ataacgttcc acaagcattt caggataaac gacatttcct tttgatttag
     1381 acatttttcc gtctttcatc aatagccagc catgcccaaa tactttttta ggtagaggca
     1441 aatcaagggc catcagcatg attggccaat agatcgtatg aaagcgaacg atttctttac
     1501 cgaccatctg aacatctgca ggccagtatt tttcgaatag actttcatct tcggagccat
     1561 atcctagtgc agtaatataa ttggataatg catcgatcca tacatacacc acatgtttcg
     1621 gatcactctt gactggtact ccccaagtaa aagttgttcg tgaaactgca agatcctcta
     1681 atcctggttt gataaagttg ttgatcattt cgtttttacg agattctggc tggatgaatt
     1741 cgggatgttc ttcatagtat tccaatagac ggtctgcgta tttgctcatg cggaagaagt
     1801 atgattcttc ttttaccaat tctacttcgt gtccacttgg cgctttacca ccgatcacgt
     1861 taccttcgtc atctttgtac acttctgcaa gttgtgtttc tgtaaagaat tcttcgtcag
     1921 aaacagagta ccagccttca tattcaccta agtaaatatc tccctgttct aataaacgat
     1981 caaatatttt ttgaacagct gctttatggt aatcatctgt cgtgcgaatg aatttatcat
     2041 aactgatatc aagcgttttc cataatttct tgacatcagc cgccatttta tctacatatt
     2101 cttgcggttt tacacctagt tcttctgctt ttgtttcgat tttttgacca tgttcatcca
     2161 ctccagtgag ataaaacaca tcaaagccca tcaaccgttt ataacgagca attgcgtcac
     2221 atgcaatcgt agtatacgag ttgccgatat gaagtttgcc gcttggataa tagattggcg
     2281 tagtgatata aaaagtttcc ttttctgcca cggggctctt cctcctttat cgcatgatgc
     2341 gaattcgttt ccttgctagt ataccataaa aaaagcatgc tgttagcttt ttttcaaaat
     2401 gaaaaagact ggataaacga cttctatcta cgttcaatct atcctttaaa aggatgaata
     2461 ggttgaataa atgcgggttt ttgttttgtt tcatatctac taaaaaacgg ctttttattt
     2521 ctcccctcac acgtggtata ataaagacta tctatcttag taagggactg gaacaatcat
     2581 gcagaagaaa catctttttt ttacacttag tattgctttt ctttcattag cacatttaat
     2641 attcagctat ttttatattc gtatgtatgg ttattttaac ctccatgggc atttgaacag
     2701 ttttatgaca gcagcttgga ttttgcgttt tatcattgac gtatatatcg ttatttgtgg
     2761 cttctttgcc attcgtgaag agcggtataa agtattgccg ttttatctgc ttttcttttt
     2821 attcaatctc atacttccat ttatttttca tatttaaatg ataacctaga tttttcaagt
     2881 aaatccttgg ttttttttca gagtctgcta caataaggta aaaagtactt ttactgtcaa
     2941 tggaggtggt cagatgaacc gttttgcttc taaagaagaa gaaaaattat attatgaaca
     3001 taaagcaagt gaagaagaat ttcttaactg gtatcaaaaa caggaacgtc cagaatatga
     3061 caagccctct ttgactgtcg atatcgtact gatgtgctat aacaaagaag aagatcagct
     3121 gaaagttttg ttgatccaaa gaaaagggca tccttatcga aattcatggg cattgcctgg
     3181 tggctttgtc caaaaggatg agtcaactgg agaaagcgtc ttaagagaaa caaaggaaga
     3241 aactggtgtg gttatttcaa aagaaaatat tgagcaactg catacattca gtacgcctaa
     3301 tcgagatccg cgaggctggg tcgtcacagt cagctactta gcctttattg gagaagaacc
     3361 gctaatcgcc ggagatgatg ccaaggaagt acgctggttc acactagaac gtcatagtaa
     3421 caagatccat ttatctagcg gagaagtggc tatctcgctt gatttagtca ctggagagtc
     3481 ttctggcaaa gatactttgg cttttgacca tagtcaaatc atcttgaaag cattcaaccg
     3541 tgtagtcaat aaaatggaac acgagccgca agttctacaa gttttaggta aagatttcac
     3601 gattacggaa gctcgaaaag tatttgctaa attcttaggg atcgattata aaacgatcga
     3661 tcattccaat tttaagaaag ctatgctcca atatttcgat gagatcggtg aacgtcctgt
     3721 aggaatcggc cgtccttcaa aaatctatca gctcaagccg gggcaccatg aataaggttg
     3781 tgagcaaaag attcagacct aatcattcaa gaaaaaacgc aaaaacaact ctttgtgagt
     3841 tacgtttttt gtttcagaat tttgatgtat tcgtaaactt tctgaataaa aaattaacaa
     3901 tagcagaaaa agctcgtacg aactttttct gctattgttt tattggtttg tcataattag
     3961 tgagcttttc ttcaaaagaa aaattcctag aaacaaaagt cattaagact ttttattaac
     4021 aaggctaacc ctaacgctcc tatcagtttc attcattttt tatcccttga taaccgaatg
     4081 tctgcccatt ttttctacta cttggctctt tcttacggaa ttgctttctt ctgctccgtc
     4141 ggtattgatc aaaagaaatt ttttattata ccattgggac ttataacgca attttgttat
     4201 taatttatgc cagtcgtgaa tagaaaaatc agcatgtgcc tcttcttttg gaaaagattc
     4261 tacaaataag tagtcatatt gtttgaccaa tttcccagat aattgttcaa gataatcttc
     4321 tcgctggttc ttttgtttca gataaagatc cattactttt ctcttttgtt tctgataatt
     4381 gcttgcctga tctaacctga tctttctatg gtgagcggag cggactttca cattcaattt
     4441 ccgctgtgca tgttgcagct tttcttctgt ctggtgaaat tttggtaaag ttacttctag
     4501 aggtcgagat gtttggatca atgtcttcgg atgataggcg atcccgatcc atttagctaa
     4561 agattctttt tttactggcg aaacatcaca aagtagtgcg acataaaatt cttcattata
     4621 tcgagcagaa atcgtagcgg agcgaatcga tccttcaact ggccgatgct gatttatctt
     4681 gattagttct ttttgttttg gtaactttaa gtagccattt tccagataga tcgtacgggt
     4741 ttggttgttg gtcgtataag attgccacat actttttttc gttttaagct ttgggaagct
     4801 ggcacgtccc ttgaaataat tgcgaaaagc ccgatccaaa ttaagctgtg catttgctaa
     4861 agcaagactg tctacttctt ttaagaatgg gtattctttt tttaatgtag cgggtgtgag
     4921 cgaaaaattg gctactcctg tttgttgcca acttttctgc cgctttttca gcaaatggtt
     4981 ataggtaaat cgagcacatc caaaagtacg aatcaattgc tgtttttgtt cttctgtcgg
     5041 atacatacgg aatttaaaag cttttagcat ctatgtccct cctagttagc aaccagaaga
     5101 gattcctttt gtcgttttag tttttttgcg taatatggga cttcctgctc agttgcatag
     5161 acaaaatgtt ctggtgcgat ttcacgaagt tgacgtactt gtccgtcatc tggttcaggt
     5221 tgatatttga ttcttctgcg ttttctctca tggtctggat ctggtagagg aatagcagat
     5281 aacaaacttt ctgtataagg atgaacaccg tgattataga tttcatcact tgttcccatc
     5341 tctaataaca agccgttatg cattacaccg attcggtcac taatatgttt aaccatcgac
     5401 aaatcatgtg caataaatag ataggtcaat tgatgttctt tttggagatc ttgcaacaaa
     5461 ttgaccactt gggcttggat cgacacatct agtgcagaaa ttggttcatc acaaatgata
     5521 aaacgcggtt ttactgccaa tgctcgtgca ataccaatac gctggcgttg cccacctgaa
     5581 aattcatgag gataacgagt accgtggctt ggattcaatc caacagttct caatagatca
     5641 tcgacttttt ttgcacgctc ttcagatgat ttagctagtc cattaacatc aattccttcg
     5701 gcaatgatat ctctgacttt catacgagga ttcaacgaag cgtatggatc ttgaaagatc
     5761 atctgaacat catgccggaa tttcgtcaaa ccttttttat ctttgatctt cgttatatct
     5821 tgaccgtcga ataatatctc accatcagta ggttcattca agcgaatgat cgtacggcca
     5881 gtagtggatt ttccgcttcc agattctcca actaaaccga atgtttctcc ttcataaatg
     5941 tgaaagctga tgtcatttac agcatgtact tcattctttt ttcctacatt gaaatactgt
     6001 ttcaatcctt taacttcaag tagtttattt ggcatcagca attcctcctt gaccgtaagt
     6061 atgtttttgt ttctctttaa agatagccca gcgacgttgg atttcttctg gcggtgtcac
     6121 tttgggtgcc tgcggagcaa gtagccaagt tgctgccttg tgtgtttttg atagttcaaa
     6181 aaatggtggt tcttgttctg catcgatttt catcgcgaat tcatttcttg gatagaatgc
     6241 gtctcctttt ggcgggttca ataaatccgg tggagaacca ggaatagcat acaatcgatc
     6301 gttcgcacta tctaatgtcg gcattgaacc aagcagtccc caagtatagg gatgttgcgg
     6361 gttgtagaaa atttcttccg atgttccaac ttcaacgatt cttcctccat acatgacagc
     6421 gacacgatca gcgacattcg ctactacacc aaggtcatga gtgataaaga taattgatgt
     6481 atcgattttt gtctgaattt ctttcagcag ctccaaaatc tgtgcttgaa tagtcacatc
     6541 aagcgctgtt gtaggttcat cagcaatcag tatttccgga taacatacca gtgcaatcgc
     6601 gatcacgatc cgttgacgct gacctcctga gaactgatgc ggataactgt tgatacgttt
     6661 ctcagcttcg ggtattccaa ctaatttaag caattctaat gcagcttttt gtccctcttt
     6721 ttttgaaact ttattatgct ttctaaggga ctcagcgact tgcttcccaa tgggcatcgt
     6781 cggatctaaa gaggtcattg gatcttggaa aatcatcgct atttctttac cacggatttt
     6841 ttgcatctct ctttctgatt ttccaacgat atcgttacct ttaaagagga tttgaccagc
     6901 atctatattg gcgttgcttg acagcaagcg catgatgctt ctggtcgtca ctgattttcc
     6961 acttccagat tccccaacga tcgccaatgt ttctccttta ttcaaatgaa aattgacacc
     7021 gcgaatcgct cgtacctttc cagcatacgt atcaaaagag atttctaagt ctttcacttc
     7081 taaaattttc gtcatcgtct tcactctttc attttaggat caaaggcatc acgcaatcca
     7141 tcagcaagta agttaaagcc aatcatgata accgataaag ttactgccgg aatccataat
     7201 agataaggca gatacaggaa tgtcttgtac ccttcgctca acatcgttcc taatgaagct
     7261 gacggaggcg taagacctaa accgataaag cttaaaaagg cttcgaagaa aatagcagat
     7321 ggaatactaa acatcatttg aataataatg acactagaga tattcggcaa aatatgtttg
     7381 aatgcaattt tcattcttga ttcacctaat gtcaatccag ctaaaacata ttcttgatct
     7441 tttaatttca ttgtttgtgc acgaacgata cgtgccattg gtatccagtt tgtgatggcc
     7501 atagcagcca cgattgaaaa aattcctggt tcaaaaacga ccaacatcaa aatcatgaca
     7561 acgaggtttg gaattcctga taagatttcc aaaaaacgtt gcatcacgtt atcgacacgt
     7621 ccgcctaaca aaccagaaac caacccgtaa gctacaccaa tcgttacatc tagtaatgca
     7681 gcaataaatg cgatcagcaa cgagattctc attcccataa ataagcggct taatacatca
     7741 cgtcccaaac cgtctgttcc taaaaagaaa tttacgtttg aagggacgtt tgcttgagca
     7801 tatttatcaa caagttcacc accaaccatt gcttttccat tcaatccatc gatattgatt
     7861 cccggaatac gaggaggcaa attgatataa gccacgtttt gggcagttgg atcatgagga
     7921 gaaacaaaga ttgtgatcac agagatcaaa atgatgacca atagtaaaac cattgaaata
     7981 acggcagctt tgtttttctt taaacggcgc catgaatctt ggataaaatt caaagaaggt
     8041 gcagtaatct gttcacgttc ttcttcggta tttttttgta atggaagaaa ttcatccgca
     8101 gggatatctg caactgtttc ataacgcttt ggtatcattt ccatttatcc tcggctccct
     8161 tctgaaacac gaattcttgg atcaactaga ccgtacaata gatccacgat caaaataaca
     8221 aaaatcagca taaatgagta aaggatcgtt acagccatga tcgtaggata atcatttgtt
     8281 gtgattgact taacgaattg ttccccgata ccaggaatag caaagatatt ttctaccacc
     8341 agtgatccgg tcatcaatgc tacagctaac ggtcctaata aagtcatcaa tggaatcaaa
     8401 ctatttcgaa gaccatgttt aaaggcgatt tcccaacggc tcaatccttt tgcttttgct
     8461 aattccacat aatcactatg cagtacttcc accatttccg tacggataaa ccgagcagag
     8521 tcagcaagcg gcgacatggc caacgcaatt gttggtaaga ttgtgtacac aaagccatcc
     8581 cattttgcga ttggcaaaac ttgtaatttg attgcaaaga tatattgtaa taaaacagca
     8641 aatacaaagt ttggaatgga acgacctaat atcgctacga gtgtagaaga tgtatctgcc
     8701 catgtatttt gcttcatggc agagatcgtt cctaaaatcg tccctaggac tgttccaaag
     8761 ataattgcct gtaaccctaa ctgcaaggat ggtccgattc ttccagctaa caaatgtgcg
     8821 actggctggt ttttaaattg gaaggaaatt ccaaaatctc cttgaagcaa attagaaaga
     8881 taaatcccat attgtacgat aactggttta tccaaaccga cttgtttatt tagcatctcg
     8941 attgtttcag gactcaaacg ttcctgattc gtatatggcg tgcccggcag caattgcatc
     9001 aagaaaaagg tgattgttgc aattaaccaa agcgtgatga tcatgaaaaa gacacgtttt
     9061 aatacatatt tgatataact attcactaat gtttccccct agcttttgac ttctgacaga
     9121 tgtttcgcta cattattaga taatgaacca aaaataaagg agcaatcaaa tgaaaaaaaa
     9181 atctatcccc tacgcagtag cttttctact gattctggtt attttaataa aaaatctcat
     9241 caaccattcc tttacactca ttcaattatc taatgatcta ttcttatggt cattgccgtt
     9301 tctgattatc ggcggttttt tatgggtctt ttcttcgggt ttcttcgatc attttcagcg
     9361 atcggtccat cttgcacgaa caaggaaccg aaagaaaaaa ccagaatttt cctcgctctc
     9421 gtctgccagc tacggtatgt acagcttttg gctgatcatt gccggaatac taattgctct
     9481 ttctgctatt ttcatgcttt tttctttgtt aggataagtg aatatgagaa cgaggttgcg
     9541 acatttgccg cagcctcgtt ttttctcatg ctcacggtat taacgattat tcttcaacgt
     9601 atgtccattt gtaatcccat gaagcgcctg ccgcatggtg aacgatacct ttgatttttt
     9661 cattcactag gtgaccttct gctttttggt acaccggaac gacacccata tcatcagaga
     9721 tgatcttgat tgctttttgg aaatcatccc aacgggcttg ttcatcgcca gcgtctgttg
     9781 atgaagctgc tttaactgct tcgtcatatt ctgcattgct ccagcgacca cggttgtatg
     9841 aattacctgt gacaaacaaa tcagtaaagc tgcttgggtc tgcatagtca gcagcccatc
     9901 cgcctagtac tgcgtcaaaa tcacccgaag ttgagcggtc aagacgtaca gagaatggaa
     9961 caggtgttgg tttgactgtt acgccatcca agttttcttg gattgagttt tgaacatatt
    10021 cgattacttt tttcgatgaa tcatcatcag aagccatcaa atcaaattct aaagaatcaa
    10081 cgcccaattc ttttttcgct ttttcccaat attcttttgc tttatctttg tcataagaaa
    10141 ctaattttcc agcttctttt gcaaagtctt cgttcgttgt tggacttttt accatatctg
    10201 atgggatcaa tcctgtagag acaactgatc cgtcacctag tacttgtttt actaaagctt
    10261 cacgatcgat agaataagaa atagcttttc ttaggttcac attgttgaat ggtgaattct
    10321 tttcacgttg attgaattcg atatagcttg tccgcgcttc tttaacagaa gtataagctg
    10381 gatcgtttgc gttttgctgt gctaactcac cagacaagat cacgtcatct gcttggccat
    10441 ctttgaataa gttcaatgca gtagaagatt cttttactac actgactttg atttcagaaa
    10501 gttttactgt atctttgtcc cagtaatgat catttttctt ataagtccat tctgtatctg
    10561 ttcctggtcc atcaaaatct tctaatgtga atggtccatt ataaacagct ttatcacttg
    10621 cagatgcata ggcatcccca ttttcttcca ctacggattg tttttgtggg aaaaatgatg
    10681 ggaaagcaag cagatattgg aaatatggtg tttgtttcgt caatttgatt tctaattcat
    10741 agtcaccaac tgctttgatc cctagttctg atttatcttt ctttccagct gtaatgtctt
    10801 ctgcattttc aactggcgca tacaggtaag catattcaga agctgtagct gggtctactg
    10861 ttcgttgcca gccataaacg taatccgctg ctgtaacagg atcaccattt gaccattttg
    10921 cgtcttctcg taatttgatt ttgtaagtta atccgtcttc actgacttct gccaactcgc
    10981 tagctcctgc cggctgtggt ttgctgtctt catcaagacg gtaaattcct tcataaacgt
    11041 tattcaatgc tgtaaaactg attgtatctg ttgcaactga taagtcagct gtaggcattt
    11101 cttgaggcac gactaagtta aatacgcctc ctccatctgc tgaaccgctt tttgctgaac
    11161 tactgcttgt ggctgaactg ccgccataac atccagcaag tacaaggccg cataccgcaa
    11221 ccaaaccaaa tgtaaatttc tttttcattt tacgtacccc tttattggaa ttttcagaat
    11281 aatta
//
LOCUS       DAZKLA010000093        11261 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000093 DAZKLA010000000
VERSION     DAZKLA010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.094"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(36..749)
                     /locus_tag="ACG85N_002096"
     CDS             complement(36..749)
                     /locus_tag="ACG85N_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0224824.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            924..2348
                     /locus_tag="ACG85N_002097"
     CDS             924..2348
                     /locus_tag="ACG85N_002097"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0224825.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2647..3354
                     /locus_tag="ACG85N_002098"
     CDS             2647..3354
                     /locus_tag="ACG85N_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0224826.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3341..3664
                     /locus_tag="ACG85N_002099"
     CDS             3341..3664
                     /locus_tag="ACG85N_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0224827.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3753..4790)
                     /locus_tag="ACG85N_002100"
     CDS             complement(3753..4790)
                     /locus_tag="ACG85N_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0224828.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5111..6094)
                     /locus_tag="ACG85N_002101"
     CDS             complement(5111..6094)
                     /locus_tag="ACG85N_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFQ0224829.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6509..7279)
                     /locus_tag="ACG85N_002102"
     CDS             complement(6509..7279)
                     /locus_tag="ACG85N_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0224830.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7272..8207)
                     /locus_tag="ACG85N_002103"
     CDS             complement(7272..8207)
                     /locus_tag="ACG85N_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224831.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8523..10172)
                     /locus_tag="ACG85N_002104"
     CDS             complement(8523..10172)
                     /locus_tag="ACG85N_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0224832.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10289..>11261)
                     /locus_tag="ACG85N_002105"
     CDS             complement(10289..>11261)
                     /locus_tag="ACG85N_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0224833.1"
                     /translation="KVYKDSLQETLVVYGKAQLPKDLKDRLSEKPEMEEISYEISFSL
                     GESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIAQIQNGFIENTPDVIPPNWSETM
                     VEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERIENQLIITLFSTTGELGKPNLAW
                     RPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNVEEGRFNEFKVAKQAEKRLEQSI
                     SYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIELPEDLIVSSIYPSYFHEQGFVLR
                     LANPTEQEKIVPEAILSLGTVVNALENKQELTTIPPYDYLSILINER"
BASE COUNT         3342 a         2310 c         1982 g         3627 t
ORIGIN      
        1 aaaaaataag ctctgaaaaa ataattttcc cgagcttatt tttcaacaaa aacagcttca
       61 aaaacaaagt cttgatataa atgtctcgat accgaatact cgaaaggttt accagacgat
      121 agagcaacaa ctttgtctat ctcaatcata aattcacctg gatctatttt taatattttt
      181 gcgtcaaatt cattcgcagt atctcctttg atacgtatgt gcgaagtctt tatttccaga
      241 cccagttcat ttgtgatata agcatataca gatttcttta aatgttttgg ttccaagcct
      301 ggtatgactg ctaatggcat gaatgtttgc tcaatcgagt atggttgaca atcaataaaa
      361 cggcttcgta caatattata tacatagtct tttacactga tacttaattt atcagctact
      421 tttttcgatg gaagttcaat tgagaaagta tggatttctg aatctacgtg ttcttcacca
      481 accacgctaa acaaaccttc gtttggacca agaactcgtg agttatcaat tggtgttctg
      541 acgacttcag tcccataccc acttctactt cgcaaaactc cttcggaaac aagtaagtct
      601 acgcctttct ttactgttaa cttgcttgta tgataaataa ctgccaattc atctcctgta
      661 ggaagtttct caccaatttt atactctcca tttacaattt ttttctttat atctaagtaa
      721 atttccatat acttcgcaat ttttttcaat ggtgaccctc caatcaaaaa catatcttca
      781 tctatacttt aacataaata aaccgataaa atccacatca aaattataca cacaataaaa
      841 aagatatata tatttaaaca aaactattta ctttaagttg taagcggtgt tatcattaag
      901 tggaaacagg aggtaaaaaa attatgacaa ctatgaaaaa gctaactttt ccagatgatt
      961 tttggtgggg ttccgcttgg tcagctgagc aagcagaagg aaaaggagaa acaggaaaag
     1021 cagagactat ctgggatcgc tggtttaaag aacaacctta ccggttttat caacgaatcg
     1081 attcctctat tacaaccgat cacatccatc aatataaaga agatatagaa ctaatgaaaa
     1141 aaactgggca taactccttt cgcgtttcta tttcttgggc acgaatgttt ccaaatggcg
     1201 gtaccggaaa agtaaatccc aaagccattt ctttctatcg aaaattattt tccgaaatga
     1261 ataataacgg aatcaaagta tttgccaact tatatcattt tgacatgcct gctactcttc
     1321 aagataaagg aggatgggaa tcacgtgaag tagttgatgc ttatgttcat tacgctgaaa
     1381 cttgttttaa agaatttggt gacttagttt gtcattggtt taccttcaat gaaccacttg
     1441 ggcctatttt aggtacttat cttgaagatt ttcattatcc aaacctcatt gactttaaac
     1501 gcggagcgca agcagcattc ttcactatac ttgcacacgc aaaagcaata caagtattta
     1561 aagaactagc tcttcctagt aaaataggcg ttattctaaa tttgagtcct acttttcctc
     1621 gtagtcaaaa tcctgctgat gtgaaagcag cagaaatcgc tgatttattt tatacgcgga
     1681 gctttttaga tcctatggtt aaagggaatt tttctaaaaa gcttatcctg ttattacaag
     1741 aatataatca gcttcctgaa gattatacga aagaagatct tgtatgtatc tacgaaaata
     1801 ccgctcaaat tcttggacta aactattacg agccacgtcg cgtcaaatct cgttcttcta
     1861 tggtggatac atctagtcca tttttaccag agtggttttt cgaaccacat atcatgcctg
     1921 gacgacgaat gaacgaatac cggggatggg aaatttacga aaaaggaatt tatgatttgt
     1981 gcatggatat caagaaccat tatggaaaca tcgaagcatt tatctcggag aacggaatgg
     2041 gcgttgcgga tgaagaacgt ttcatggatg atacagggca aataattgat gattatcgga
     2101 ttgagtatat caaagatcat cttgcttact tatggaaggc aataaatgaa ggatgtaaca
     2161 ttaaggggta tcatctttgg acatttattg attgctggtc atggatcaac gcctataaaa
     2221 accgatatgg gctggtttca ctcgacctta ctactcaaaa aagaacaatt aaaaaaagtg
     2281 gcgaatttta caagcaatta agtgatacaa atggatttag atacgatgta gacttgttag
     2341 ttgaataata ttctaaaatt ccacttgaag tgaacacatt agaaaacaag aataatgaga
     2401 aataccaata acaaatactt tttcttcata cttcatttat acttgtcagt ataagagact
     2461 gtgacaaatg tagtgatcct cagaagttag gtctggaaat ctaacttgag aagatttttt
     2521 taaactacgt ccagtctttt ttatttgttt aaaattgaag ttgacaagtc ttcattaaaa
     2581 atttagaata ttcacaagtt tgacgacaat tatgaaaatt tatgtcgtgc taaaggagtt
     2641 ttgttattgg atgaaaagtt agatataaaa actgcgatca aagacacgtt acctaccgtt
     2701 tttggttata tcggtattgg acttgcattt ggtatcgttg ggaaagctgc cgggtttcat
     2761 ccgctggtcg tcacgttgat gtccctgctg atctatgctg gttctgccca atttatcacg
     2821 gtcagcatgc ttgctagcca tagtccactg ctctccatcg ttctctcgac ctttctagtc
     2881 aattcccgaa tgattctgat gagtatgacg attgctcctt atttcaagaa aaatcggctg
     2941 cttcaaaatc tgctgattgg cacattgctg acagatgaaa gtttcgcact gggaatgaat
     3001 aaattgaact atactggaca aaagttaaac tttcgttgga tgaatacagc taattggatt
     3061 tcttatttga cgtgggtggg ttcttctctt gttggcgcac ttttagggaa ttttattact
     3121 gatccgaaaa agtttggttt ggatttcgcc atcgtcgcta tgtttatcgg gttactttat
     3181 cttcaagtca tttctgatag aaacacctct aaacgattac agttgatcct gattggtctc
     3241 actttgattc tggtatatgt cggactgatt tttatcccaa gtaacttagt gattttagtt
     3301 gtaacactga ttgggtgcgg attgggggtg tggatcaaac atgccttctt ttgagtttac
     3361 attgctgact attttaggtt gtactttagc aacttggctt tctaggatcc taccatttgt
     3421 cttactgaaa aaattcgatt tgccgcaacc gttgcttgag tatttaagtt ttgtcccgat
     3481 cgtcattatg tctgctttat ggttcagcag tctatttacc caaaatatcg gacatctgcc
     3541 gcagatcaat ctcgaaaatg ctttggcttc agttcctaca cttttagctg ctattctttc
     3601 aaaaagctta ttggtgatcg tcttggtagg aattctttcc ctttcgttga ttcgcttatt
     3661 ataaataaaa aaacaagagc ttatggcaaa ttactgtcat gcgctcttgt ttttatcaaa
     3721 gtcaaataag aaaaaaatcg gcgtattctt gcttatccgt tgaacggacc actaacaacg
     3781 acttttccta atatatgtcc tgtttctacg tctttggttg cttgtttcaa gttcttcgca
     3841 ttcatcccat ctgaatagac cttgctcaat gtactgcgga taatcccttc atttgctaaa
     3901 gcagcaatgt gcgctaaagc ttctccttgc gtttcgattt catagttata gtctgtttta
     3961 gcgaacatat actcccagtc gaagctgata gattggtttt tccaaacacc tatatctaac
     4021 ggctctttga tccctacgat cgttcccaca tgtccaaatg gtcggacaag gtgtttgatt
     4081 tgatcaaagt agcgggtaat atcgaataat acagcgacat actctacttg atcgattcct
     4141 aattcacgaa aacttttact taagtcgtga tgataatcga ttggatagtc tacacctgtt
     4201 tttttcagcc attcaaaatt cttcggactt gcagtcgcgt agacttctag gccagcccaa
     4261 tgcgctagtt gattcaaaat cgaaccgacg cccccagctc cgttgatcac taagattttt
     4321 ttcccgcggt tggcattttc ttctggagtc agaccaaatt tttcaaataa caattcataa
     4381 gcggtcaatg aggtcaacgg caaggctgcg gcttcttcat ctgaaagatt ttttggtgca
     4441 agagcgacaa tacgttcgtc aaccagttgg tattcttgat tgcttcctgc tcttgtcgtt
     4501 gtacctgcgt aaaaaacgcg atcgccgaca gaaaattttt gcacttcatc acctacagca
     4561 acaactttcc cgactccatc aaaccctaag atacgtaaag catttcttat cccgttatct
     4621 tggcgaagtt tcgtatcgac aggattaaca gatactgcgt tgacttttac cagaagatcc
     4681 cgtgaactgg gtacaggtat ctgttctttt tcatcaataa agctttttgg atcatcgatc
     4741 gacaaccctt cataaaaacc gactaccttc attaattcct tttctgacat acgaattcct
     4801 ccttagcttc atcaagttgg agcatccata cagttttcat ttcaatatat atagtatgct
     4861 atttgttcca cttttattca tcataattat tctcttactt aaagtaaact atttgcactt
     4921 ttttggttaa aaaagaggtt gcgacaagac ttttgtcaca acctctttta aacggtgttc
     4981 aatcaactga ccctcggtat tcgctcaaaa aacgacaaaa tatgaggagc tatttttgtt
     5041 tggctgttct tattacttga tgcaggacag ctttttcacg acctctaatt cacggtgttc
     5101 aagcaagaaa tcattgttca ctgaaaagcg ttggatgttc tttttcaaag gtgtctaaga
     5161 tctctcgtac ttcatcagta gatttcgtat tcatcaactg aactcgcaag tcatttgctc
     5221 ctggaaatcc tttgatatag attttaaaga aacgatgtag tcctgtgatg gaacgcggga
     5281 tgatttctga ataatgatct tgcagatcta gttgcagacg aagtagtccg atcagttctt
     5341 tttctgtatg ttccctcggt tctttttcaa atgcataagg attcttgaaa atcccccggc
     5401 caatcatgat gccatctacg ccatattttt cggccaattc ttctcctgtc tggcgatctg
     5461 ggatatctcc gttgatcgtg atcagtgttt gcggagcgat ttggtcacgt aaagcaatga
     5521 ttttagggat gacttcccaa tgtgcatcta ctttactcat ctctttacgc gtacgcaaat
     5581 ggacagaaag attggctata tcttgatgaa gtaggtgact gatccattct tccatctcag
     5641 acatttccgt atagccgata cgcgtcttga cgctgacagg caaaccgcca gctttagccg
     5701 cttcaatcaa ttgtgccgct acttctgggc gaaggatcaa gccactgcct tttccacgtt
     5761 ctgctacatt agggactggg cagcccatgt tgatatcgat tccttggaag cccatttctg
     5821 ccatagcaat gctcatttct ctaaaaaatt ctggcttgtc tccccaaata tgggcaacca
     5881 tcggctgttc atcttctgta aaggtcaatc ttcctcgtac actgtctttt ccatctggat
     5941 gacagaaact gtctgagttt gtaaactcag taaagaagac atccggtgct gctgcatgct
     6001 ttacgacatg gcgaaataca acatctgtta catcttccat tggtgctaaa acaaaaaaag
     6061 gctttggcag ctctttccaa aaattagtag tcatttattt gttactctcc attctatcaa
     6121 gcagtgaagt cgcaatcttt ctcaaaaaca ttgtcattat actaggaaac aaaaaaagaa
     6181 gcaaagtgaa cagcttaaaa gatgtttctg attgagcaaa aaaagactgt gacaaaaatc
     6241 ttatcacaat tccttttccg gaataaatgg tgttcaaaag cttgcatctg cggtaataag
     6301 tccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat tacttgatgc
     6361 agaacagctt tttcacaacc tctccactaa acggtgttca atcagctgac cctcggtatt
     6421 agctcaacaa acgacaaaat atgaggaact attttcccgt ttgtttctct aatactcagg
     6481 tcaaaacgct gatttcacaa cctcttttct aacttctcaa tcctcttcct ttattgatca
     6541 aataccaagt aacggcgtac aaaacaacga tgaatagcac aagtacgatc atcgatgttg
     6601 ctactggcac atcgattgtt ccaaggaatc cgtaacggaa accggagatc atataaacga
     6661 tcggattgac tttcgaaaca gcttgccaaa atggcggcaa catagagatg gcatagaaaa
     6721 cgccacctag ataagttaac ggctgcaaga caaatgtagg cacgatcgat acatcgtcat
     6781 aagactgagc aaaaatcccg ttgagcagtc ctgctaacga aaacaagatt gccgtcatca
     6841 ataatgtaat gatgaccatg aaccatgaat acacatgaag tggtacaaag aacaaggaaa
     6901 tgatcgttac gagagagccg acgagaatac tgcgacccac tccgccaatc acaaagcccc
     6961 aaatgatcac atgcgtagga acaggcgcta ccaagatttc ttcgatgttt ttctggaatt
     7021 tttgagagaa gaaggacgaa gaaacgttgg catacgaact agtgatagca gacatcataa
     7081 tcaaaccagg cacgatgaat tccatatatg aaaaaccacc catgtcaccg atccgaccac
     7141 cgatcatttt tccgaaaata acaaagtaca aggatgtcgt gatcacaggc ggaaccagtg
     7201 tttggaccca gatacgcaaa tagcggttcg tttccttgat agctaaactt tttaatgcag
     7261 taaaataaag attaaacatt tttctcctcc gtatgattat tttcttctgt gattttcaaa
     7321 aacagttctt ctaaacgatt cgacttattc ctcatagaaa gaactttgat tccttgtctt
     7381 gtcagctgat cgaagattcc gttcacccct tgatttcgtt caacttcgac agcgattgtc
     7441 tgttcatctt caaatgtaca agcgtatcct tcaatcacag gtttggtctc atatggcgct
     7501 aaatcaaaga taaatgtttc aaattgcagt ttggatagaa gctctttcat actagtattc
     7561 tcgatcagtt cacctgattg gatgatcccg atgttgcgac aaagcatttc agcttcttct
     7621 aaataatgcg tcgtcaaaat gatcgttgta ccattttcgt tcaattctcg gagaaactcc
     7681 cacatctcgc gtctcaattc aatatcgact cctgctgttg gttcatccaa gatcagcaaa
     7741 cgtggttcat gcatcaatgc cctagcaatc atcagtcttc gcttcattcc cccggaaagc
     7801 atacgtgcac ggacatttct cttttcccaa agattggact gcttcaaata tttttcgctg
     7861 cgtttcatcg cttctttgcg agagacaccg tagtagcctg cttgattcac gacgatttgc
     7921 tggaccgttt caaagggatt gaaattgaac tcttgcggga ctaatccaat ctgttgcttt
     7981 gctcggacta gatccgtatc cagatcataa ccgaagacat tcacttttcc agatgtttta
     8041 ttgactaaag aagtaatgat tccgatcgtt gtagacttcc cagctccatt tggtcctaat
     8101 agcgcataga aatctccttc tttcacggaa agatcgattc ctcgcaatgc ttcgacccca
     8161 cttccataaa cctttttcaa attatttatt tcaagcgcat ttgccattta tttcctatct
     8221 ccttatagac atgattcttt attgtatcat ggaatacagc agatgaaaaa acaacttggt
     8281 tccagcgaaa aatcaggttg ctgggctcag gaaaatctcc taatgaaaga catcaatcgg
     8341 acgaagtcag acaacctttt cagaaaaaac aaatcgaaga taaaggatta ctattcatat
     8401 gtgcaacaaa aggagcgtga gacaaaagta aagaacactt ttgtctcacg ctctaaacac
     8461 gtataaacgg cggaagcaaa agccaagatt tttgatccaa taaatacttt cgatcacttc
     8521 gcctatccca atacatgcca ctgccgcttt tcatcaaata acgcggcttc cgtttttgcg
     8581 gtttctacta aagtctctgg gtaatccaaa actttcagca aggcaatcgc atttcgggtc
     8641 cttgaaggtc cttcttttaa tttgaaatca aaactgattc cttttccttc tgttacttgt
     8701 tcttcaaagt ggacattctc acaataattc tttaaaatct ctgtcaattc gatatcatgt
     8761 gtggcgacaa atgctaaaga tggatactcc gcaagccacc gaacgactga agcggaagaa
     8821 gcaatccgtt cgatcgtatt tgtccctttt aaaatctcat ctacgaagca gtagcagcgt
     8881 tctttggaag ccacttggtc caacaaacgt ttgatcgatt tgatttctga aacaaaataa
     8941 ctgtcgcctt caaataaatc atcttcgatt gccatggaag tcaacacatg tccaggttgc
     9001 agggtaaact gttctgcaat cgctgtttgg atcgtctgtg ctagaataca gctgatggct
     9061 atacttttga catacgtaga ttttcctgat gcattggaac ccgtgactaa tgtattcttc
     9121 tgccagttga ccggattcac taccgcttct tttaacaatg gatgatagat atctttcgct
     9181 gtcacaccgc cctcagtaaa ctctggtcgg caagtgatgg gcatataagt acggtaattc
     9241 aatattgcag cggctacttc taattctccc agcaattccc acagcttgat ggcttcagtt
     9301 gaatgctttt ctaatttagt gagaacgaaa tgataggaga taaatggcag catgaacatg
     9361 atgttgacat attcaaaaat caactctgct tcactcccac tcttcatacg aaatgaaaaa
     9421 cctaatttag ggatgtctct taatggttta gtttgctgtt tcagctcttc ttgaagtggt
     9481 gtcggcagtt ttgctagctg attggcggct gaaatcgttg aaaccaaata actcatgcta
     9541 ttcagctctg tttctagtgt ttgtttcttc actagataat agaccgtatt gaatactaac
     9601 ccaccgattg ccaaatagat tcctagtact tgtcctaaaa gaagcaaacc caaacccacc
     9661 agcggaagca agccaagaaa aataaataaa cctacgcgtc caatcttttt actttttcca
     9721 tccgctagat attgttttgc aaaattatga tctttttttc ctaaacgagc gaattgatat
     9781 tggatttttt ctctcagttg aggattttcc tgataaaaag caatcagttt ttctaactgc
     9841 tgatcttcac caaaatcaaa gctgcgtaaa cgttgataca acgcttctga acctacacta
     9901 gaataggtac tgttgattcc ttcgaatact tcgaacatat ccaggtcata ccaagtaaga
     9961 tcatcgattt cgctatccca tgaacggaac ttcttctccg tgagccatgc ttccttcaag
    10021 ctttcttctt tatcaaatct cggttgataa ggaatttttt cccatttgct acgaactgtt
    10081 tcctttaatt tcaaacgtga aaaaatgtct aaagcaacta ataaaataac agctgcaata
    10141 ataattaaaa cgataaattc tctttgaccc atatccctct ccgttccttt atttgttagc
    10201 tttagtatag cactgttgcg ctatttgaaa agagggtaaa aaaacacctt tcaaaataaa
    10261 aggtgttttt ttcttgtata ttacatattt atctctcatt tatcaaaatt gacaaataat
    10321 catagggagg aattgttgtt aattcttgtt tattctccag tgcattgaca actgtcccaa
    10381 gggaaaggat agcctcaggt actatttttt cttgttctgt tggattagct aagcgaagaa
    10441 caaatccttg ttcatgaaaa taagaaggat aaatcgatga tacaattaag tcttcaggta
    10501 attcaatcaa tgaaaattta cgaggaacat caagttcatt ttgtaatggc caaatcttgt
    10561 tatctaacct gtgaataaat acattcagtt tctgcttttg ataagaaata gattgttcta
    10621 accgtttttc tgcctgtttt gccactttga actcattaaa tcttccttct tctacattaa
    10681 atccaaaagt gactttatac tcacctatct cttgagctag cggtgtttcc atcatgacat
    10741 gcccttcatt tgtcgtatct cctgaagctc gcccaggtcg ccaagcaaga ttaggttttc
    10801 caagttcccc tgttgttgaa aataaggtta tgattaattg attttctata cgttcatact
    10861 cttttaaacc atctgcataa aacgtaaaat aatgatcatt ttctgccaca gagacactct
    10921 tatcgaatat ttctaaatta acaggtttct ctaccatagt ttctgaccaa ttcggaggaa
    10981 tgacatcagg ggtattttca ataaatccgt tttgaatttg tgcaattgat ttttcagatt
    11041 tatctaaaac attaattgct aagcgcatac gatgcgagta aacagtatta tgaattttga
    11101 gtgtgccttc aacgatttga ctttctccca gactgaacga tatttcataa gatatttctt
    11161 ccatttcggg tttttcagat aaacgatctt ttaaatcttt aggaagttga gcttttccgt
    11221 aaacaactaa tgtttcttgt aaagaatctt tatatacttt a
//
LOCUS       DAZKLA010000094        11244 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000094 DAZKLA010000000
VERSION     DAZKLA010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.095"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..1076)
                     /locus_tag="ACG85N_002106"
     CDS             complement(33..1076)
                     /locus_tag="ACG85N_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0224834.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
     gene            complement(1237..1920)
                     /locus_tag="ACG85N_002107"
     CDS             complement(1237..1920)
                     /locus_tag="ACG85N_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFQ0224835.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            2115..3044
                     /locus_tag="ACG85N_002108"
     CDS             2115..3044
                     /locus_tag="ACG85N_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0224836.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            3105..3896
                     /gene="phnC"
                     /locus_tag="ACG85N_002109"
     CDS             3105..3896
                     /gene="phnC"
                     /locus_tag="ACG85N_002109"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224837.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            3868..4647
                     /gene="phnE"
                     /locus_tag="ACG85N_002110"
     CDS             3868..4647
                     /gene="phnE"
                     /locus_tag="ACG85N_002110"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0224838.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            4644..5426
                     /gene="phnE"
                     /locus_tag="ACG85N_002111"
     CDS             4644..5426
                     /gene="phnE"
                     /locus_tag="ACG85N_002111"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0224839.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            5429..6232
                     /locus_tag="ACG85N_002112"
     CDS             5429..6232
                     /locus_tag="ACG85N_002112"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0224840.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            6316..7863
                     /locus_tag="ACG85N_002113"
     CDS             6316..7863
                     /locus_tag="ACG85N_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFQ0224841.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(8160..8357)
                     /locus_tag="ACG85N_002114"
     CDS             complement(8160..8357)
                     /locus_tag="ACG85N_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224842.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(8433..8633)
                     /locus_tag="ACG85N_002115"
     CDS             complement(8433..8633)
                     /locus_tag="ACG85N_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0224843.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            complement(8753..9940)
                     /locus_tag="ACG85N_002116"
                     /pseudo
     CDS             complement(8753..9940)
                     /locus_tag="ACG85N_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9985..11111)
                     /locus_tag="ACG85N_002117"
     CDS             complement(join(9985..10883,10883..11111))
                     /locus_tag="ACG85N_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0224844.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT         3269 a         2200 c         2242 g         3533 t
ORIGIN      
        1 actgtagaca tcatccataa aatggacttt gtctacagtc tgaaatcacc actgatgatt
       61 ttctcttttg cgtctgaaac tgcctgatag gcttcctctg ataattgtcc ttttgtcaga
      121 tctacccctt cttctttcaa accaaaggtc aaatgcttgc cgccaggaaa tttatcgtta
      181 tgagaagttt ctgcaaattc tttgacttct gcaccgacat ttttctttgt agaagtcaac
      241 gtgaaattcc ctgtctcgcc gttctccatt ttataatccc cttcatccgc ttgatcgcga
      301 tctacaccaa tcacccatac ttttctgcta tagtcgtttt cattgcgggc ttttgcttct
      361 tggaacaccc cagctccagt acttcctgcc gcctgataaa gaatatcgac tccctctttg
      421 tacatagaag cagcaatagc ttttccttta tctagcgaat cataggatgc agcatattgt
      481 acgttaacag taatttcctt gttcagtgct gtggcccctg ccttaacgcc agcttcaaat
      541 ccgctttgga aacgttggat cacgggccct tccattccac caataaagcc tacttgatca
      601 ctttttgtcg tatatgcggc agctagtcct gctaaatacg cactttcttg atctttaaag
      661 gttgctgatg agatattctt tttcccagta atctcaccat ctaccaaggc aaatttcaac
      721 tttggattat ctttagcagc attttccagt tcctctgctt gcaagtagct caagctaata
      781 atggtggtaa agccgtttct gattgcctga tcgatattgg tactgttatc ctctggattg
      841 ttcgtttcta tatacgtaaa atcttttttt gctgtcagct ttttctcttt tccccaagcc
      901 tctaatcctt cccagccagc ttgattgaag gagcggtcat caaccccgcc aaatccaaga
      961 accaaagcga ttttactttt ctctttttca ctttgcttct ccccctgaga ctgactacag
     1021 ccgactaacg ttactaatga caccaaaaac aacaatgaaa caagtatttt cttcaaaatt
     1081 tttctcccct ttttatgtgt cttgacacat tgaactttta aaagtatagc ataccaatat
     1141 atagaacact actcttcaac gaagattttc cgtttcccaa tttctccact atcaaaaaac
     1201 cagcaatcac ttctgactgc tgttttttta tagctgttat tctgctttta ctggactact
     1261 tatattctca gctaagtcct ctaaagcttc gatatcatga atcaagtaat aattcttttt
     1321 cttttcgata atattctcct cacgaaaatt cgtgattgtt cgtaaaagat gtcgataact
     1381 gacatttaag attgaggcgc aggtggttaa ccgaaatgag aaacgttcgt tttcgtgatg
     1441 ggtcaaaata aactttgcta aacgagtttc aacaggttct gtcagtgagt tcgccaaatt
     1501 tatcccggaa tttaaacgat aacttagtaa ctggcaaata ttctgcaaaa agcgaacatc
     1561 tttttgaagc tgtgttcgat attgatttag cggaatgctg atacagatgc acttagtcac
     1621 tgctttaacg ctgcttttcg gcgataattc ccacaatgaa gaggcttcgc cgagtggtgt
     1681 agcgggcttt gcgtaatcga tgcaaatatt ttgtgtatca gaagaatacg agtacaccat
     1741 taccgtccca tccactaaaa aatacaaata atctgaggtt tttcccgttt gaatgaggtg
     1801 ctcttccttt tcaaaaaaaa gtaaagaagc cattgccaat atatctgaat ccatataatc
     1861 ctgcaggttg tgcttctcaa cgtaagcagt taattgcttc gtatcatgga tttttttcat
     1921 gaaatcgtca cctctaagaa gtttttcttt attatagctt agattgtttt tcggctcaat
     1981 acgaataatt atttataatt gtttgtaaat tgtatgtaaa agttgtgtaa aataggacgt
     2041 gcgtcacttc attgattttg attccctgct atgatttaga cgtattagaa attatatcca
     2101 acaggaggag aaaaatgcag ttcaaacagt tagcaaaagg gttcatcgga gtcatgttat
     2161 tgaccacttt ggcggcctgc ggttctggga atacaagtag cagctcagaa aaagagagca
     2221 aagaaaaatt agttgttcaa ttcgttccaa caaacaatga tggtagtatg gaggcaaaag
     2281 caaaaccatt tgctaagtat ttatcgaaaa agttagatcg tgaggtagaa gtgacgttag
     2341 ctactgatta ttcaacgatc gtcgaagcga tgtcttctgg tcaagtcgat gtcggaatta
     2401 tgccgcctgc agcctatgtt caagcaaagg atgctggagc tgcagatgca atcctgactt
     2461 ctcagctcgg cgattatgat caagaaactg gcttgccatt aaaggacaaa cttacgaaca
     2521 cctttaaagg agaagtattg gttcgtgccg atagcggatt gaataagtta actgatttaa
     2581 aaggaaaaaa tatcgcaacc ttaagtccta actctgctag cggctacatc tatcctgtcg
     2641 ctgaattgaa agatgctggg attgatccta ctgcagatac aaagatgacc actgtgaatg
     2701 atattccgag tgagattacc gcagtgttga acaagcaaat ggatgcggcg tttgtttttg
     2761 aaggcgcgcg aaatgttttt gcttcgaatt tcccagataa tgatttattt aaggatttaa
     2821 aagtcctgta cctgacaaaa ggcgatattc cgaatgacgc aatcgctgta cagcctgaca
     2881 tgtcaaaaga tttaaaagcg aaaattaaag agaccttctt aaatatgaag gatgataaag
     2941 atggcaaaga agcaatggct ttgtgggggc atcagggcta tgaagaagct gctgattctg
     3001 cctatgacac gatcagagac tacacgaaaa aagcagcaga ataaaacatc agtaagtgtc
     3061 agcaagcttt tttttgctta cacttaaaaa aatggagaag gataatgatt atttttgaca
     3121 atgtttcgaa ggtctatccc aatggcgttg ttggcttgaa ggatattgac ttaacaattg
     3181 aagatggaga atttgtttcc atcatagggt tgagtggtgc tggaaagagc actctgctgc
     3241 gcagtatcaa tcgcttggca gaaaccacca aaggagatat tcgcattaat ggaacctcta
     3301 ttacaaaagc gaataaaaaa gagctgcgac agattcgccg ccagattggt ctaatctcgc
     3361 aaagcttcaa tctggtaaag cgtagtacgg ttcagaaaaa tgtgctatct ggacgtttgg
     3421 gctattactc cacttggaaa agtattttag gtctttttac gaaggaagac taccagcaga
     3481 caaaggaagc actggaaaaa gttggtttgt ctgataaatt gcagacacgc agtgatgagc
     3541 tcagtggtgg gcaacaacag cgagtatcca ttgcgcgagc actcgtgcag caagccccca
     3601 ttattttagc ggatgaaccc gttgcatcat tagatccaat cacgactcaa aaggttatga
     3661 tggacctaaa aaatatcaat caaacgatgg ataaaacagt cattgtcaat ctgcattcag
     3721 tccctctggc tcgtgagttt tctacgcgtg tgctcgcgtt aaaagctgct caggttgtct
     3781 ttgatggtcg tccagacgac ttaaccgatg aacgcttaga gcaaatttat ggcaatgcaa
     3841 tctttgaaga aaaggcaggc gaaatcgatg agcttgaaag aaaccgttca ctttaaatgg
     3901 tataaacacc tgcttattct tctgttctta attgtttgtt ttcaaggaag tgcctggatc
     3961 acgtcagctg atttttctca ggtttttcaa aactccaatc aaatgactgt ctttttatca
     4021 cgcttcattc atccagactt tagctattta cctaaattag tcgagcctat ggtgaaaaca
     4081 cttcaaatgt ctttgcttgg aacggtcatt ggcttaattt tagcgatccc tgttagtttt
     4141 ttagcgacca ctgtagtgac agaaaaccgt cttctttcta ccatctttcg ttttctttta
     4201 ggtttagtac ggaccatccc taccctgctt ttagccgcgc tttttgttgc tgtgtttgga
     4261 attggtgaag cgactggcgt gttgactatc gctgtcttta cttttggtat ggtgtcacaa
     4321 ttaatgtttc aagcaattga aaccattgac tttggcccga ttgaagccgc tgcggctgtt
     4381 ggagcaaata aaatgcaaat cgctatttgg tcgatcgctc cacaagtttt gagtcagttt
     4441 gcgagctatg ctttttacgc gtttgaggtg aatgttcgtg cttctactgt ccttggatat
     4501 gtcggagctg gtggtattgg cgttattcta aattcctcac ttgcgctttt gaattatgaa
     4561 cgagtttcaa tcattattct aacgatccta gtaactgtcg cgatcgttga taaattaagt
     4621 gaacgtgtca ggaggtccct gttatgatgc ttcgtgaaat gaacagaaga acccttttat
     4681 cattagttgc tttagtgatt ctcgttattt tgtcggccgc tactctggat tactctggat
     4741 taagcacctt ctctccttcc atgggaatgg acgttttgag agggttaggt cagcctgatt
     4801 ggcagtttct atatgatggc agcggcgagg atttgattag tttgctatta ttaaccattg
     4861 gaatcgcctg tttagggaca atgatcgcta cggtattagc gattcccatt accttgatca
     4921 gtgcggttaa cctctggcaa gcacatccat gggtaacaaa aattggcaag cttatctgta
     4981 atgttttacg agcttttcct gaactagttt ttgcaattat ttttgtgaaa gtcgtcggac
     5041 ctggcccctt tgctggtgtt atggcgattg gcgtccatca gatcggaatg cttggcaaac
     5101 tttttacaga agaaatggaa gcgatggatg agcgacttgt tgaagaaatg catgcggtag
     5161 gagcaaattt ttggcaaacg attttctttg ttcgcatccc ctatttgatg ccgatttatt
     5221 gttctttagc attaaatcat tttgaaatag cggttcgtag tgcggctacc ttaggcttag
     5281 ttggcgctgg cggaatcggt gctccattga tattcgcgat tcaaactcgg tcttggagca
     5341 aagtgagtat tattttgatt ggcgtagtcg taaccgtgtt tattttagat caaataaccg
     5401 gaattataag aaagagattg agataagaat gaatatattg catatatcag atatacactt
     5461 tcgcagagag tatgaggctt gtgaagaagg ctataaggga atgctggcta cgatgcagaa
     5521 tccccttatc cctctggagc aatgcattca tcatctcctg cagcaaacac ccttggacct
     5581 tgtgattatc agcggagatt taaccgaaga tggcgaaata gacgattatc gatacttgaa
     5641 gacttggctg gaaaatgcgc taggagaaac gacgattctt gttactctag gcaatcacga
     5701 tatcaaagaa catttttggg taggctggtg cgatgaagcc gcctcttctg ccccgtacaa
     5761 ccagatagtc aaatatcctg agtttacagt gatttccttt gataattcaa gctatggata
     5821 cgcagatggt atagtggacg atcagcaatt tcaatggctg aaaaaagcct tagagcaaga
     5881 aaaagaccaa ccgattttct tggtcaccca tcatcatcta ctgtctcacc aaagcagtat
     5941 accaaaatgg cctggtaccg aacgtttctt gaagctaatc gcaccttacg atattcgctg
     6001 tatcctaaac ggtcataccc atcacacatt tactgatgaa attaatggga tcccctattt
     6061 tactgtatcg agtatgtcct ttgttggtga agatgaaggc gatggcttcg tacgctttga
     6121 agaacgtcat gggtataacc tttatcatct cgatcaagga aggattgttc gccagacaac
     6181 agaaaacttt attccaggca acattttaaa aacacttcaa atgaaggatt aaggcattat
     6241 aaatcaaaaa aagagaccga acagctgaat acgcttcggt ttttctattt tgagtcagct
     6301 aaaggagcac gcagaatgaa cattacgttt ttagagacaa gtgatatgca tggctatgtt
     6361 tcaccaacag actattccgg cagaaccgat ctggccttag gaacggctaa agtatcgtcg
     6421 aaactgaagg aattacgggc aaaagcagat ggaccggtaa ttacgatcga aaatggtgat
     6481 ttcatccaag ggtctccttt gagttattat cttgctaaag aatatggttc agccaaagag
     6541 cttacagact tgattaacca aatgagctat gatgttcaag tgttgggaaa tcatgagttt
     6601 aactatggtg tggattatct cgaggaagcg attaagaatt atcaagcgcc tatattagga
     6661 gccaatatgg tgaacaaaaa tgaccaacca tactttggaa acgcctataa gattatcgaa
     6721 aaggtgggtg taaaaattgc tatcttggga ttaacgacac aatacattcc tcactgggaa
     6781 aagccagaaa ccttagcagg aattacgttt aagagtatcg tcgaaacagc aaaagaatat
     6841 gttcctctat taaggaaaca agcagacttg gtaatcgtta gttaccacgg cggatttgaa
     6901 aaagacttgt taaccggtga ggacacagaa gcgctaactg gagagaatga aggctacgag
     6961 ctattgcagc aggttgaagg cattgacgcc cttttcaccg gtcatcagca ccggaaaatt
     7021 gctgaaacgg ttcatggagt tcccgttgtt caaccaggct atcgtggcga gttcatcggt
     7081 gaaattcgtc ttaccgtcga gaaaaaagag gggcaagtgg ttgttacaga tcaccaggtt
     7141 gctttacact cagttgaaaa atcagagcct gatccagaaa ttcttgcagc gatcgtaccg
     7201 gtagaagaaa aactagagaa atggttggat caacccttag gtaaagtgaa cggaaatatg
     7261 cgtattaatg atccgatgca ggctcgtctt gaggaacatc cctatgtaga gtttattaat
     7321 cgtgtacaat tatttgccag cggctcagag atttctggaa ctgcgttatt caataatgaa
     7381 ggaaaaggct ttggtgagac gatcacgatg cgggacgtca tcaccaacta tatttatccg
     7441 aatactctgg cagtgattca aataaatggt gcagatttga gagcggcgtt ggagcagacc
     7501 gcaaattatt tagctgtaga aaataaccaa atcgtattca atccagcctt tattcagcct
     7561 aagccccagt actacaatta tgacatgtat gaagggattc agtacactat tgatatgaaa
     7621 cagcctgttg gacaacggat taccgaactt cgttttaaag gtcaccccgt tcttccgcaa
     7681 caaatgctgg aagtggtgac aaatcaatat cgggcaatcg gcggaggaaa ttatgcgatg
     7741 tttggtgcgg agaaaattgt ccgagaaatc caaatcgaca tgaccgaatt aattgccgac
     7801 tatttgaata ttcacccacg catcgaagca aaagtgaatc ataattttca agtgattttg
     7861 tagtatataa ataaaaaatt caaaaaggtg tgagcagttt tttgcccaca cctttgttat
     7921 gccaaattgt taggctgtct gatttccatc cagccattct ttagccagcg atctacctca
     7981 tcaaaacaaa aaattttcct ctgatggttc cttaaataag actattttga tcctttttcc
     8041 atgaataaga ttttgcatca gacaatactt catttctgaa aaagttgcgg caccaatata
     8101 gcctaatacc cctttccgat cttgattaaa tacaaaaaat aaatccgctg ctgcgatatt
     8161 taaaaaaaag tcgtcaagat ttctggatac tctttgtcta ggatcggaca tgagcgagga
     8221 taatcaatta attcaatgcc ttttttactg aaatcttttt ccatatctcg atattcttct
     8281 tggaaaacaa tacttccaga aataacagct ttcacaaaat tggcctccat ttttttattt
     8341 ttcagacact ccaccatacc ggatacctct caaagaaaag acatagcaaa aatcaatagc
     8401 agctatctct ccacggtttt ttactggtaa ttttattttt cttttggaaa ttgtatcagg
     8461 aaggtcgttc cttcattttt tcggctggtc acttcaattg aaccgtggtg caggcgcact
     8521 agttccttca cgattgataa tccaagacct gactcgccta tctttgtatt tttacttgac
     8581 ggatcggctt tataatagcg atcccaaatg tttttttgtt cctcttcaat catgccgatg
     8641 ccattgtcct cgacagtaat aaatacaggc tctttgtcaa atgatgttgg taaagagaaa
     8701 tcttagaata atttggacag aacagaaaga agaaactctc tttctgttct gtttatttgg
     8761 ttaaaaatgt tttgccatga cgaatcatga tccgtagttt gaaattgtaa aagctgcgaa
     8821 agccgtacgc attgcgtttt aatactttga tgtggttgtt taaacattcc aatggtccat
     8881 ttgaatagct gtagtgaagc gcattttgga tctgtggcag aaacttttta aacgttcgta
     8941 aagtggttgt ataggtttca ggaagaccgc tgacttctct gtttaaacag tcctcaaata
     9001 atgactgatc tcttttcttt accgcataaa gaatgtcttg gtataactga tagccttggc
     9061 gcaggttggg acaagcagat aacagtcgat cgacaatctc actttcagtc aaataatctt
     9121 taaaactggc acgccaaacc cttttttcgt agtttaactt tgcttcttct ttttgtagga
     9181 gtttccaata gcgtttcaat tttttcccta accctcgttg agagtgggaa gttcctttaa
     9241 gccgtgccgt ggtttctttc actcgatgat tcaagaaggt tctgccaata tgttggacga
     9301 tgtggaaacg gtcgatgatc aactgagcat tcgggaaaag ctttttcacc aatgaaacat
     9361 aaggcgcata catatcaata acaatccatt gaacggcttc acgagtcgct aatgaaaacc
     9421 gagaaaaata gcattctaaa taaggcaagc gtcgattttc gacaatatcc aataatgcat
     9481 ggctttgtcc gtccatcatg ataaaactca ttgcactggc aacacttcga accgatttga
     9541 attcatcaaa gcataagacc tttggcaaag cctgtttgaa aggattcaat gggcggtaga
     9601 atcgcttcat gacacgatag accgaagaag tggaaatatt tttctgacga gcgatttccg
     9661 tcatggaagt attctctttt aagcgagcaa tgacagacca acgaactttt tgactaatga
     9721 aacagtgttt ttcagcaaca gaggtatccg ctacgaatgt tctttggcaa gacttacatt
     9781 taaatcgttg tttcttcaac tccagataag tgcgatactc agacgcatca ttcagtaaca
     9841 accgaacggt tgtcgtcccc caacgaatga tcctagaggg gacgacacaa agacaatggg
     9901 aacactcttc tggcttgtac agaagagttc ctttgtaaat catggatttg agtcaatatc
     9961 ttggacacat tttagtagag acggttaagc cattttttct tttgctaatt cttcaaattc
    10021 ctgtggggtt agatagtgaa tcgagctatg gattcgattt cggttataaa atccttcaat
    10081 gtagctaaat agcgctcgat ttgcttcttc aaaatctgag tagctagtcg tgtagacttc
    10141 ttcttttttc aatgaggcat ggaaagattc gattccagca ttatcataag gtgttccttt
    10201 tctactataa gaatgcctta ttttgttggt tttaagccat tgttccactt ctcttgctgt
    10261 gtattggctg cccaagtcag tgtgtagaat cattccttct ggaatgtgat aatgtatttt
    10321 tgctttattc aatgtttgaa tgacacaatc cacagtcatt cgttttgaaa atgtataact
    10381 aataattttt ttcgtgtgta aatccatgat tgaagataag taacaccaac catttttctt
    10441 agtaggaatg tacgtaatgt cagctgccca tttctcacag atcgtttcag tagaaaagtc
    10501 ctgatttaag agattctctt ttgaaatgat cggtttatta gacctttgag gtctatattt
    10561 tttaactaca atcgacctta aatttaattg tttcattagc ttttggacat gtttaagaga
    10621 tactgaaatc ccttctttta ataaaacttg atggatcttt gtggcaccat agcgttgttt
    10681 attcgtaaaa aaaattgttg aaatcttctt tttcaactta ttatttctga tttctgtagc
    10741 tgtcaacggt ttattcttat aaaaataata gacacttcta gggatttcta ataaccgaca
    10801 taacaaagaa acagtgtaat ccttacacca tttttcaata aattgaacaa gcgaatttag
    10861 gttttccgag agaatatggc tgcggctttt tttaagattt caagttcctc atttaaacga
    10921 gcattttctt ttctcagttc agctacttct tttccagtca gtcctgtgga ctgatcaggt
    10981 aaatataaat tcttccattt gtaaatcgtt gcttcagata agccatattc tttcgctaac
    11041 cctcgaacag aacgtccagt ttgattcagt tcaacaatca tttgtttaaa attttcttca
    11101 tatcgggtca taaaaattca tcctctcgta gataagttta atttattctt gtctctacga
    11161 aaagctgtct aagatttgat actaacacca agttatcttt agaaccattt cagatacata
    11221 tgaaacaatt acatatgatt tttt
//
LOCUS       DAZKLA010000095        11228 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000095 DAZKLA010000000
VERSION     DAZKLA010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.096"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(190..930)
                     /gene="truA"
                     /locus_tag="ACG85N_002118"
     CDS             complement(190..930)
                     /gene="truA"
                     /locus_tag="ACG85N_002118"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0224845.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1121..1756)
                     /locus_tag="ACG85N_002119"
     CDS             complement(1121..1756)
                     /locus_tag="ACG85N_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFQ0224846.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(1903..3123)
                     /gene="sstT"
                     /locus_tag="ACG85N_002120"
     CDS             complement(1903..3123)
                     /gene="sstT"
                     /locus_tag="ACG85N_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFQ0224847.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            3342..4340
                     /locus_tag="ACG85N_002121"
     CDS             3342..4340
                     /locus_tag="ACG85N_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224848.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            4536..6371
                     /locus_tag="ACG85N_002122"
     CDS             4536..6371
                     /locus_tag="ACG85N_002122"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0224849.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(6431..7147)
                     /locus_tag="ACG85N_002123"
     CDS             complement(6431..7147)
                     /locus_tag="ACG85N_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFQ0224850.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(7374..7916)
                     /locus_tag="ACG85N_002124"
     CDS             complement(7374..7916)
                     /locus_tag="ACG85N_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFQ0224851.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(7921..8148)
                     /locus_tag="ACG85N_002125"
     CDS             complement(7921..8148)
                     /locus_tag="ACG85N_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFQ0224852.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(8227..10107)
                     /locus_tag="ACG85N_002126"
     CDS             complement(8227..10107)
                     /locus_tag="ACG85N_002126"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0224853.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(10296..10910)
                     /locus_tag="ACG85N_002127"
     CDS             complement(10296..10910)
                     /locus_tag="ACG85N_002127"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0224854.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            11052..>11228
                     /locus_tag="ACG85N_002128"
     CDS             11052..>11228
                     /locus_tag="ACG85N_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0224855.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYD"
BASE COUNT         3496 a         2254 c         1814 g         3664 t
ORIGIN      
        1 tctaaacaaa aacaaccgag aagagaagta caacattctc actagatttt tatttaagaa
       61 agacttttta gctgtacaca taaacaggct aaaaagtctt tttttatatt tctttaattt
      121 gcttcataca ttatttaaca tcgatattta tactgtgaaa catgcgtttc atccattatc
      181 aaagtatttt tatcgataat aaacttcatc caaaaacaga ccttgtgcag ggacggtttc
      241 tcctgcatgt tgccgcactt tgctttcaaa tatttcatcg attgtttcta tcggcatggc
      301 acctgtgccg atttctagta atgtccccat gataatccgc accattttat ataagaatcc
      361 gtctccgaca aaagtaaaat gaagcatttc tccctttcgt tcgatagata attcatcaat
      421 cgtacgcact gtggattttt ttgatttttt caatgcagaa aaaccaatga aatcatgtgt
      481 acccactaat ttttcgcaag ctgcattcag cttgtccata tccaactctt gagggaaata
      541 aaagctgtgg tttcgctcaa atgctgaagg aatcacgttg ttccaaacat aatagctgta
      601 ttttttgccc accgcattat atcttgaatg gaagcgctca ggcatttctt cgattctttt
      661 gatgacaata tcccgcggaa ggtaacgatt cataaagtcg atcatttctg ccagtccaat
      721 ttctgtgttt gttttaaaat ttgctacttg tcctcgcgca tgggtgccag catctgttct
      781 tcctgatcca ataatttcta tctcttcatt tgtcatctga tgcaatactt tttcgatttt
      841 tccttgaatg gttttatcgt catctccaag tctttgccat cctgaatatc tttttccgtc
      901 atattcgatt gtcaacttga tgtttctcat attttagtgg ctcctctgtc ttccatttgg
      961 tcttctctct acctttttaa gattgtagca caaaatatag ctccttgctt ctctaagaca
     1021 gaagctatac aagaaaactg tggaaatata tatttcagaa aaacaaaaaa cagccaccgg
     1081 atgaagtatc cgctgactgt tcaatttgac ggctcaagtt ttactgtttg agatgcaaga
     1141 tactcaacca ggcaatgggc acataattaa agaaaaaact gattctttgg caaaaaccta
     1201 ttttattcag taatggccaa gaatttatag aaggaattct tgccagtcca taaatcgccg
     1261 caaaaagcaa actgatcaca gacagtccaa ggtataagtg gcttacgtcc atttgcccat
     1321 tttttcgata gatcaagaaa agaagaactg gaaacaataa aaaaccactg taacctaaac
     1381 ctgatccgat attatggacc catgtcgaga cattccacga agattcatgg gtattgatac
     1441 tgaacaaacc tgtaaagata caatcaccta taccatagaa cgcgatggct gctgtaacta
     1501 gggtagctaa cacacgagat gtactaacaa atgcagcata caatgcaggc aatgacagga
     1561 tgaaaaagat ccctgaaacc acagaccaaa taagaaacgc ttttcttaca ggactggaaa
     1621 cctctccaaa tacactgatc acgctcttca tttgatctaa ttcaggataa tacaatccta
     1681 atacgtacgg agtcaaaaaa tcactcagca cgcctaataa taaaaagaaa aaaccaaatt
     1741 tacgtaaaaa atccatgata cgcctccaat aacaattacc tatgctttta attgttatta
     1801 taaacgactt tatcttgatt taacagaaac ctctatttac acgaacatct atataactca
     1861 ttaaaaactc gaatcatttt aaaaatacta aatatctaac gttcaacgtt cacttaatcc
     1921 atctaaacaa tctttcaatt tgattttttc cccacttctt ttacgcgcgc ctagctctcc
     1981 tattgcagta aacaacagat cacttgacga atttaaggca gtttccattg aatcctggat
     2041 aacaccgata ataaatccaa taccgacgat ttgcatcgcg acatcattag agataccaaa
     2101 caaactacat gctaatggaa tcaataacag tgaaccgccc gcaacacctg aagctccaca
     2161 agcagctaaa gcagacaaaa tacataaaag gactttcaaa acaaaaggtg cttcaatccc
     2221 taaagaatgt acagcagtca acgtcatcaa ggaaatggta atcgcggcac cgcccatatt
     2281 gatcgtagca cctaacggga tcgatatgga ataagactct tttgtcaatt tcaatctttc
     2341 agccaacatc atgtttatcg ggatatttgc ggcagaactt cgggtaaaga aagcgggaag
     2401 tgcgctttct ttcaaacaaa agaaaatcaa tggataggga ttttctttaa taaacaaaaa
     2461 cgtgaggaat gggtacacaa ctaatgcgac aaacagcatc gtaccgatca aaacaagtaa
     2521 aagctgcaga tactctgcca atccagcgat tccagtcgta gcaattgatt gataaaccaa
     2581 tcctaaaatc cctatcggag caaatgcaat aatcacttgt acgacttgtg acaacgctat
     2641 agatatctga tccaccaact cttttgtcga ttctgaacgt gctcgaaaag ctagtcccaa
     2701 caatacagcc cagaataaga cgccaaggta attcgcatca ataagagatt gaacaggatt
     2761 ttgtgtgaca ttcgtcaaca tagtagataa aacactttcc aaactttgcg gtgctttttc
     2821 agatactgtt tcttgtaaag taataggaac agtaaaaatt gaacttacaa taactgctac
     2881 aagtgcagaa agcaatgttc ccactaaata taagatcaaa ataggcttca cgaatgtctt
     2941 attcccaatt ttatgcttgg caattgacgc catcgtcaag aaaaaaacca gaataggtgc
     3001 gattgccttc aagcaactga caaacaacgt tcccaatatt gaaataaatg tccatgtggg
     3061 gaaaaagata ccaaacaata ttccgactaa aataccaaca acaatacgtt gaactaacga
     3121 catatccaaa aacttctcta acaaatgacc accctttcac aattttaaca atcattgtat
     3181 cattttatgc aaagaaacta aaggtataat tttctaaaaa ttctaacaat acaaacaacc
     3241 atttcttgct aattttgtac tttttctgtc tacacgttgt tttttgagat attctaagtt
     3301 aggatgttat taatgggcaa cttatcagga aacgttttct tatgtttttg tccaattttt
     3361 ttgtgaggtg tttattcttg aaaaaaataa agaaggagag ttatggcttc ctgacaatct
     3421 ttgtcgcttg tataatgatg gttttacagc gtatattgat ttcagcagtc actattccta
     3481 catatgcaac aaaccctggt cgattctaca tctatacatt tttacaaact tccgttgtta
     3541 ttggtgcaaa tttgatccca ctttacatgg gttaccaata tcaaaaaatc gcccgtttaa
     3601 aagttttaca ttatttaagt cgttttgcct ttatattcct aatagccaca ctagtttgca
     3661 atatcttttt ttacgtacaa gctggcagct tgaatattcg tgattattgg cttatcctaa
     3721 cacctatcag tcagaattac tttacttatg ctgtttcttg tactttgctt ttttgtagta
     3781 ttccttatac agttggattt ttaaataaat tatcgaaaac aacaattaaa tatctactat
     3841 tatttttaac aattggatta gttggctgct ctactctatt taataaagac ccgtttgcat
     3901 tacagaatgg acagagtatt ttatggattt tttatttatt tttaatgggt tatggtttga
     3961 aagaatggaa ctggaaagaa aaggtccgct ataagcttcc tcaagtattt atcagcttga
     4021 ttgttttatt cggtttgatt attttgatga cacaaatttc actcatcatc agaggaaata
     4081 cagatacagc gctgaggttt tctaaaccat attctctctt cgcaatgtat tatactttct
     4141 cttcgttttt atttttagaa atgctatctg ataaattagg tataaaagga agaggaaatt
     4201 ttcttgctgt atttttgatt gtcactcaag ttatatgtaa tttcccttta gtttcttatc
     4261 gcgtatctac atttatgaaa cgtgaatttc caaactcagg gaaggcatgg ctgataaata
     4321 tcgggcagtt ttttatttga tatttattag cagtgtccct cctagttatc tttttatttt
     4381 atatacaaaa aatccgtttc attcatcgtt tcatcgaata cttcgctttt gattcgattg
     4441 aacaattaac tcagcgttgg aaaaaaagaa ttcattggct ttttgttcat aaaagttatt
     4501 ttttagttgc agcatttttt tactgcttta ccttcgtgca gattttcgtt ttagaaccaa
     4561 aagaaggttg gaaagaaact atccaggttg ccttaaaaat atttactcag cgacaagcac
     4621 ccatgatcct gacaatcatc attattatgg gatttttctt cttactattg ttgatttcca
     4681 atcgtttttg gtacgccttg actgctacct tgatcattaa cttactgctc actatttcga
     4741 cagtaatcaa aatggaaatg cgggaagagc ctgtctttcc ttcagattta aaaatgctga
     4801 ccggattgtc agaactatta tcgatggtta gcccagtctt attaatcgtg ggcggattga
     4861 tccttctcct attattgatt actagtatta ttgtacaacg tagactacaa aaacagtatt
     4921 ccttgaagat tcattggaaa cgtcgattta tcagtatcgc tgtattatta ggatgttttt
     4981 cgggtgtctt tttcattaat cacaaaaatt cgccttcttt cttgctgttt aatttattta
     5041 aagtaaacaa gactttcttt aaccagcaag gcgccgtaaa agataacgga ccagtcattc
     5101 aattcctaaa caatatcgac attaaaatca tggaagaacc tgctggctat tcgaaagaaa
     5161 aaattaatgg aataatgaaa aagtacaaca caaaggcaga tgaaatcaac caaacaagaa
     5221 gtgactggtt aaacaatcag acgatcgttc tcaatttaag tgaaagcttc agtgatcctg
     5281 cacgtgtccc taatctgaca gtacctacag accctattcc ttacatcaaa agcattataa
     5341 cagaaggaac aggaggattg atgttatccg tcggttatgg tggcggaaca gctaacatgg
     5401 aatgggaagg actgacaggc ttgagtatca gtaacctgtc tgcttcgtta gttacacctt
     5461 atacccagct agtggagcgt cagaaaataa gtccaaatat taccgatcta ttcgatgaaa
     5521 aaatagccat ccatcctttt acagctgctc tttatcgcag aaaacaagtg tttgataaat
     5581 ttggttttga caggttctat tatttagaca gtccggataa attaacttat acggataaaa
     5641 tagaacaaag cccacgcata tcggatgatt cagcatacaa agaaaccttg aaagctttac
     5701 aaagcaatac ggaaacgtca caattcatcc aattatccac tatgcaaaac catatgcctt
     5761 atgatgatta ctatagtgaa ttgaactaca ctgctgaagg aactgctgtc attcctaata
     5821 gaagaaaaga attacaaact tatatgcaag gccttcacta taccgataca gcagtaaaaa
     5881 aattcatcca agaaatcgac aaaatcgata aaccgattac ctttgttttt tatggtgacc
     5941 atttacccgc aatctatagt gggaatgatt cgaaaaaata tggattagaa cagcaccaga
     6001 cagattattt catttataac aacaagtata gtcgtgaaca agcagctacg ttaaataaag
     6061 aagttgttgc tcctaataac tttggtgcta tggctcttga gcatgcgaat atcaaagtaa
     6121 ctccttacta tgcgttgttg acagaagtat ctaatcatct acctgcatta acaattgatc
     6181 ctacagaaag cttgtctaat cgttttaatg gaaaacaggt ctttgttaca gaggatagtc
     6241 aaattatcca agaaaaaaat ttaactgaga aacaaaaaga aattctagaa gattatcgtc
     6301 tgatacaata tgatcttgtt gcaggtaacc agtacagtgc aacatgggcg acacaaaaaa
     6361 taaaagacta acaaataaac tgagcaaggg gttgtgataa aagacatgtc acaacccctt
     6421 gtttatctca ttaatttgct tgtccataaa cgatttgatt aaagccattt ttcaattcat
     6481 tattcgctgt ctcgttgaag taaagataat tatcgggatt cccctgattt tgttgtgtca
     6541 ccaatacttt tggctgattt tgctgaaacc cgaatagata gacgtgcttt ttcagatatg
     6601 gttctgtctc tgcatcggaa tagacggctg tgatttgata gtctgcttga cctgtcccac
     6661 tttctgacca ttctgcagaa acaggcactc caccaatagc catcttccac tccccgttca
     6721 aaatagcttg agggacttta agaccatata agtccacact catttgattc gtataacttt
     6781 tgtattgttg tcccattgtt tggccccatt tggtcataaa tgcctctaat tgtgcggatt
     6841 tgtctgcatt ccacagtgtt tgttcaactt gttcgctgct ttctgaagag gaagatgaag
     6901 cctctacaga agaacttgaa gtagatgttt gagttgtttc agtcgttttg gaagactctg
     6961 acgaagtaga ggtatctgct gtttctgtac ttgctgcagt tgacttgctc tctgtgctag
     7021 ttgttgtagt agttgcaact gttgctttcg ctgtttcttt tgttgattct tttttcgatt
     7081 cattcccgtt ggtacaacca gctaacaaaa aagctagacc aattgttact aaaatccttt
     7141 ttttcaatta aaaaactcct ttcgattact cccacttctt tattctatca aataagcaat
     7201 gtgattttat tgatttttcg tattttctca tcaatctttc aaatcttcta aaaactgaga
     7261 agcacccgct gatgttgtct gtaatacatc agcgggtgct tcttttattt taatcttcgt
     7321 gattttccaa tttacctcac gctttatatt tgtatttgaa gaaacataga cttttagtca
     7381 tcatcgtctt cctcatccaa atcttgtcgg acagtttttc cttggagtct ttgtacttgg
     7441 tagatgattt ctttattgaa acttaaaagt tcaacaagca attgttgcaa gccatatttt
     7501 tcttcttttt cagctaattt gattgcccgt gtgataaaga cattttggaa atcaaaatct
     7561 tttgcaaagt tttccaacat tgttgaggct ggtaaatatt tgttttgacc atgttcgtaa
     7621 atttttccat aacgcatgac ttcttctaaa gtagacggaa cgatttcatt ttcaatcact
     7681 aactcatgag agattttatt gaaccatctc gcttcttgat cagcatattc tgaaaaaagg
     7741 gtacttaatt cttgcgaaga tgtacccaaa acatacaatt gtgcttggat caatttcatt
     7801 tcttctaatt taaaatttgc taatacatgc cccaccatgg cgccagcagt tggtttatga
     7861 tggtcgatat ctgcttgttt tgtttcttga acaaattttt cttccactat attcattgat
     7921 ttacgcctct tttacttttg ctgattggac ttcataacct aatttttcca caactgcagc
     7981 taattgttca gggtggactt catctggatc aaagtttgct ttgattttgc tcgcattaaa
     8041 caaaactttc gtattgctta ccccaggctg ttgagaaacc gcatgttcga ttttttgcat
     8101 acagcttgga caagctagtg tatctaattg cataataatt tttttcatcg tagttcttcc
     8161 ttcctttttc ttgatagtct tattctagat tacagcttcc ataaaaaaat tgattcttat
     8221 caagttttac gatttttctt ctttgctttc gtgtccaaaa tacaataaac gcatcgcgtt
     8281 aaagataacg atcaagatac tagcttcgtg aacgaacatc ccactagcca tgtaaataaa
     8341 cccggcaaat aagccgttga gtaaaaataa gacgacacca atggcaatca ctacattttc
     8401 acgggtattt aaaacagttt tcttagccaa gcggtaggca tgaaccaacg attcaaaatt
     8461 agattgcatc aagacaacgt cactcgtttc gatagcaaca tccgttcctg atcccatagc
     8521 aatcccgata tcagccgcag ccaaagaggg agaatcattg attccatctc caataaaagc
     8581 aacgcgcaat ccttgttctt ggtatttttt cacaaattga accttttcgt ctggtaataa
     8641 ttcagcatgg acttcatcaa tgcctaacat atcagctaca taatcagcag tcatctgatt
     8701 atcacctgtc agcatcacta actgttttgc tcctgcttgt ttcaacttag ctaattgttc
     8761 agcgacttct ggtcgaatca catctgacac acctatgatt tgagtgattt ttttgtcgat
     8821 tgctacaata atgaccgagc tgcctgtttg ttgtaattga tgtaaatctt gtacttgctt
     8881 tggtgtgaga gaaagtttgt gagcggcaat caatttggtg ttgcccgcac aaactgtatg
     8941 accatcaatc tcagctgtga ttccttgtcc cttgattgtc tgattatctt tgactgctaa
     9001 ttgttgaaaa tcaatgtctt tctcctgagc ataatggatc acggcgcgcc ccagaggatg
     9061 atcggataac gtttctaatc gagccactaa agctaagctg tccccttcat cctctgtata
     9121 gttcttgata gtggatacag cagtagttcc tttggtcaat gtaccagttt tatcaaagac
     9181 aaaagtatcg attttggaaa aagtatccat tgcttcaccg cctttgacta agatgccccg
     9241 tttcgctcca ttaccgattc ctgctacatt cgaaacaggc gcaccaatga ctaaagcgcc
     9301 aggacaacca agaaccaaaa cagtgattgc taaacgaaaa tcacgactaa tcagaccaac
     9361 aaccaacgca ataagtaaaa cggttggagt ataatactga gaaaatcgat caatgaattt
     9421 ttccgcatgg gatttggtat cttgtgcttc ttctactaat tcgataattt tggcaaaggt
     9481 cgtatcatct ccgaccttag ttgcttcaat cgtcacgtag ccatctgcta aaatcgttcc
     9541 agagaagact tcatctccac tgattttgac gacttgtcgt gattcaccag tcactgcggc
     9601 ttcatccgca taccctttcc cgtcaatgac tcgtccgtcc acaggaatag ctccaccagt
     9661 ttttactaag acgatatcgc cttcttccac gtcatcgatt tccacttctt cagtcgttcc
     9721 atcttcttgg acaacactag cagtagatgg ggccatctcg gtcaaagcct tgattgactc
     9781 ccgcgttttg gtcaatgttt tttgttccag atagctacca aacaaaaaca agaatgtaac
     9841 gatagctgac tccttgtatt ctccgatcat aaaggcacca agcacagcaa tagtaacgag
     9901 taaatcaatt gagataactt tgactttcac tgcctgatac gcatggatca taataggtat
     9961 gaccgcaata atagaagcta tggctagagc aatgttatag aacaagacaa aaccagtaaa
    10021 ttttccgata aaaccaaaaa taatcaacac agctgataag gcggtgatat gatttcgatg
    10081 ctcttggatc cacttttgta atttcatctt tctccctctt ttcctttcat tcttacgaga
    10141 ctaagtatag atacttatcc atcataaaaa attgaccact atcaagttta gtcaaaaagg
    10201 cggttttctt ttggtagaat acactacaaa aaatagttag caagaagact cactaaaagt
    10261 gatcttgcca actattgtcg tttctatcga aattttcatt gaatgatttt cctgtacagc
    10321 atacatcgga gatctatttc tttatctaag atttttatct tgctaatttt ttcctttagt
    10381 ctttcaaatc cacgtcgttc atagaattga tacgtacagc cactatctgt aaaaaggaat
    10441 atttcttttc ctttctctct tttttcaaat tctgttagca acttggtacc gatgcctttt
    10501 gttttcaaat caggatttgc agctaaaaaa atgatctctc catctggcga atgcttcttt
    10561 aaatatgctg caagcatttg ttcatttgct gtctcgtaag ctcctttact ttctttagca
    10621 aatatatttt gtaaaaaatc gaatatctta acatatattt gctgtccaaa agaacgatgt
    10681 ttcttttttt ctccttttat ctccgccagc aaaacacctg ccaactcgtc ctctgcatac
    10741 aaagcaagaa tttgagttgc tcgattcaac tccatataca aaaaatatct tccgtaaaga
    10801 ttcagcagaa gttcattttc catatatctc tcaaagttca ttccttcaac agcaaaatag
    10861 atagcttttt tgtaatcttt cttttctaag ggtttaaaag ttagtttcat catttcgcct
    10921 cctcaacttc aatgattgtg ttatacttac attataatag acacagtgtc tactatctat
    10981 aatcaatcat ctataagttt gtcgattaag cgacagcttt gctttgatat gtcggataag
    11041 gagataggta aatgaaaaag acagatagac gcgtcaaaaa aacagaaaaa gctttggctg
    11101 aaacactatc cacattatta gtaaataaaa agattcaagc aatcactata cgcgaactaa
    11161 cagaaacggc agatgtacat cgttcgactt tttatacaca ttataaggat atctatgatc
    11221 tatatgat
//
LOCUS       DAZKLA010000096        11204 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000096 DAZKLA010000000
VERSION     DAZKLA010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.097"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..705
                     /locus_tag="ACG85N_002129"
     CDS             106..705
                     /locus_tag="ACG85N_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFQ0224856.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
     gene            705..1280
                     /locus_tag="ACG85N_002130"
     CDS             705..1280
                     /locus_tag="ACG85N_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFQ0224857.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            1305..4976
                     /gene="brxC"
                     /locus_tag="ACG85N_002131"
     CDS             1305..4976
                     /gene="brxC"
                     /locus_tag="ACG85N_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFQ0224858.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            5040..8672
                     /gene="pglX"
                     /locus_tag="ACG85N_002132"
     CDS             5040..8672
                     /gene="pglX"
                     /locus_tag="ACG85N_002132"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFQ0224859.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            8788..>11204
                     /gene="pglZ"
                     /locus_tag="ACG85N_002133"
     CDS             8788..>11204
                     /gene="pglZ"
                     /locus_tag="ACG85N_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFQ0224860.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTV"
BASE COUNT         4211 a         1514 c         2121 g         3358 t
ORIGIN      
        1 tataataaat atgtattgta tagtagatac taaaaaatga atgctaacga tgagcaaaaa
       61 acacttgttt acttattgaa ttatagatga ttaggaggca atctattgaa taaaaaatat
      121 tctgctggtc tagtttctaa acgcttttgg ttttcagagt tcaagttata tattcaatta
      181 cgactagaag gatatacaga tatagaaatt aaagagaaaa atgaacatga aaatattttt
      241 tttgcagtga gcagtaaccg tcaaaaagaa atctatcaga acgtgaaaag gagagttagt
      301 caactggatg aagctggatt gcaacttttc aatcagttaa atatagataa tcaaaagtta
      361 gtgaatttga tttccatcct tatgattgat gatcttttta atgaatttat gactgaggta
      421 tatgctgaaa atctacgtaa aggaaatttg caattaacac cagttgatta ccgttctttt
      481 tttacagaga agcggagaag ttcagaagat atttcaaaat ggaaagatta tacgattaaa
      541 cggcttattg ccgcttatca aacgaatcta attgaaacag gattagtcag aggggaaaaa
      601 gaatatggac gaatgactcc gatattatta gataaacgat tgattcaatg gttagttgac
      661 aagaatcgta aagatattgt actagcactg ggaggaattt tataatgtca ttatcgattc
      721 aagagcattt ggaaaagttg aatgaagtaa ttcaaaaaga agatttttta ttgaatcgtg
      781 gtttaggtaa tgaagtaggc tattatatct ttgactacga tccgaaagac gagttaattg
      841 taagagatta tattaatgat atatctggaa catctgttgg tgataattat gaagtgaaag
      901 tttataatct ctatgtgatt atgttagagt atctagaaaa acgcggtttc actgaaaaag
      961 cgattaagat ggaagaacga cgtggtttac aaaaattagt ggaaagcatt ggtcgtttat
     1021 tgaaaatgga tttacctgag gaagaaaatg tattttttga atatgtggat gcccatacac
     1081 cagaaaaatc agttgtgttc ctgacaggaa ttggcgaatt atatccatta attagagcgc
     1141 actctatttt aaataaaatg catcaggtat ttgatcgcgt accagttgtg atgatgtatc
     1201 caggggaata tgatgaatta agtttgacaa ttcttaatac caataaagat gataattatt
     1261 atcgagcatt tagaatttag ttgggatgga aggtaggaga ttaaatgcta attaaagata
     1321 tttttgcaaa accaattgac cgaaatattc aaggagttat taaggtcggt caagcaaagg
     1381 atgaaaatgt tcaacaagaa ttagaagaat acgtggttac gaaagaacta caaaaacact
     1441 ttaaaacaat ttttgatgcc taccaacgga gtatcaatac accaacagat aaaatgggtg
     1501 tttggattca ggggttcttt ggttcaggta aatctcattt cttaaaaatt atttcttact
     1561 tgctcgcaaa tcgagaaata aatgacaaga aagccattga ttacttcaaa gaagatggga
     1621 aaatcaaaga tccactgact ttggcaacga tggaacggtg ctcacaaata aatacagatg
     1681 ttattctgtt taatattgat tcaaaaagtg atgcaggtgg taaaaaagac aaaaacggaa
     1741 tcgtgaatgt ttttttaaag gtatttaacg aaatgcaagg attaagcacg attccacatc
     1801 tagcagattt tgaacgtcgg ttaattaaag aagaaaagtt tgatgcattc aaagctgctt
     1861 ttgaagagat taatggggca aaatggcaag aagagcgcca tgagtttgat tggatccaag
     1921 atgatatcaa agaagcggca cttaaaatcg attttatgag tgaagccgct attgataatt
     1981 ttttagaaaa agcatcagga aattatttaa taagcattga tgactttgca aatctagtta
     2041 aaagctatct tgattccaaa gaagcagacc atcatattgc ctttctagtc gatgaggtcg
     2101 gacaatatat cggcggtaat cgtgagttga ctttgcagtt acaaaccgtt gtggaagatt
     2161 tgggtcgaat ttgtgaaggt aaggcttggg tattagtgac aagtcaggaa gcgattgatc
     2221 acgtaacaaa ctttgataac aattctgata atgatttttc taagatcaag gatcgttttg
     2281 ccacaactgt gagtttatct tcagcaaatg ccgatgaagt aattaaagaa cgtattttga
     2341 agaaaaaaga gtttgccaat gattctttag ccttacaatt tgaaactgtt caaacagaat
     2401 tgaaaaataa attactcttt gttgatagtc ctgaattaaa gctcttccaa gatgctgaaa
     2461 attacgcaga agtttatcca tttattccct atcaattcaa tttattgggg caaattctaa
     2521 ccgagattcg taatcacagc tatcaaggat caaacttatc gtctggtgaa cgttcattac
     2581 tagcaatgtt taaagaagcc gctgaacgaa ataaggaaaa tgatacaacc agtttggttt
     2641 ctttagatca gttttattac tcgttagaac gttggattga tacagcaatt agccgagtga
     2701 ttacacaagc tacaaataat tctcatattc aaagaccaac tgatatagat aactttaacg
     2761 taaatgtctt acgaatttta ttcatgatta aatatgttga tcaagccatc aaaccaaatg
     2821 ttgaaaatat tacgagcttg atgataagaa atatcaacga agaccgcctg gaaactaaaa
     2881 aacgagtaga agaagcattg gaccttttaa ttaaggaaaa ctttgtgcgg aagaacttag
     2941 atagttatat tttccaaact gatgaagaac aggaaattac tcgtgagatt aaaaatcaaa
     3001 atattacaga tagtgatttg atcgtagaac tatctaaacg aatttttgac ggcgttctag
     3061 accgaaatag ttttgcctat gaaaaattag gcatgtcagg tggaaataaa tttaaaaacc
     3121 ggtatacgtt tacttatgca caagtgttag atcaacgtcc atacaagcaa gctggaaatg
     3181 ctgaaatgac agttaatttt atttcaccat actctgattt agcaggtcaa gaggcagaac
     3241 ttgcgcttaa gagttcccgt aacttattat tcgtagaatt gccatcagat aaagaattta
     3301 aagatgatat agcaatgtat gctagaatga atctttattt aacaacgact tattcgagtg
     3361 tagtaaagaa ctttgacgcg attcgttcgg aaaaatctgg tgaattacag gaactaagtg
     3421 caagtatcaa cgatcaatta aatgaactgt tgaaatctgc aaaatttttc tataacggtg
     3481 cagaaattca actgacaggc tccgacttta aagcaaaatt ccaatcagca cttcagaaaa
     3541 tggctgaaga agtataccat aaattaaacc aaattgatgc gcctaaagaa aaagaagata
     3601 tcttatattt agtgaaagaa agcaaaaatg aaattattga agatccatca aatgagcaag
     3661 cagttcagga aattttcaat tttatcaatg atcaaaagag aaatcaccta acgattacga
     3721 tgaaaacaat tcgtgatcgt tttgaaacaa aacaaccgta tggattcaca gaacttgatg
     3781 tggaatggtg catcgctaaa cttttcatgt ctggtaaaat tgatttactt tataacaatg
     3841 tacaaatttc acggatggat gatccaaata gtattgtaat gattattcaa aaacgacgag
     3901 aaagtgaaaa gataaaaatt gaaccaagcg cggtgatttc gccaaaagca aaaaaattgg
     3961 ttgacgaaat cggtagagaa ctgtttcaaa gtcgtaatat tgttgatgaa aataacaatg
     4021 aacatacaat ggaaaatttc aagaaatatt taaaaggtca attagacaat ttacgctctt
     4081 attttgattt acaaaacact tatacctacc ctagtgctta tgaatatgag caaataaaaa
     4141 aatatcttga agtaattgtt caaacgtcag atgttgaaga ctttttttat acaatcaaaa
     4201 aatatcgcga tgaattattc gactgggctg aaacatatga taaaattaaa aatttccaca
     4261 agcaaaatag tactcagaaa aaaatctggg agaatgcaca agataaaatt agattaatcg
     4321 aaaaaagtcg gcgtcaaact tctagccaag aaattgatca attgattcat gagatgaaaa
     4381 ccattttgaa aaaggctgat ccttattcag atatgatgaa actaaatgag ttgattcaag
     4441 aatttattga acaatataat caactgttag agttggaaag tcaggaaata ttaaataaat
     4501 taacggaaga aaaacaagca acatttaaat tattagaagg aaaagatttt aaggatcaat
     4561 ttgtaccaat agttgaccaa gaatttcaag aactgaaagc aaaaattgaa caagcgaata
     4621 ctatagatga tcttgttttc ttatcattaa acgtagcaga acagtatttg atctttaatc
     4681 aacgtttcga taatgaagaa aaccgcagac gtcaagaaga acaagcacgt aaacaaattg
     4741 aagcagcaaa cttgattcgt gaaactgaaa aagatgaaat agactctgca gcaaaagctg
     4801 aagaaatagc gatagtaaaa cctactattc aacagaaaac agtaccaatg agaacactta
     4861 acgttcatgt taaaactatt aagtcagcag cagatattga tgagttaaca gctgaaatta
     4921 atcaaaaact gaaggaacaa cttcaagaaa atacagaaat cacgattcaa ttctagtgaa
     4981 aagacaggca taaaaaggtg cctgtctttt caaaggttac atatagaagg gaaagaaaaa
     5041 tggataagaa agcgataaag aatttcgcgg tcaatgcccg tagaaaatta attgatgaag
     5101 taatgactaa agcaaaacgt ttaggaattg atgaatcaaa aattgaagat gtgaagaaaa
     5161 ttgatagcaa tttacaagaa atcgaaaaat caggaataag aattcaagga aaagaaatag
     5221 cgcaaagaaa caaattgatt gcagaactaa aacaacgtgc ttctgtcact tcttattcag
     5281 aagcttttca agagttaatc gaagaggttg cttatacgtg gtttaaccga ttgattgcca
     5341 ttcggtttat ggaggtaaat gattatttac ctgacacgta tcgtgtatta tctagtgaaa
     5401 taagtgggaa aattgagcca gatattatta cagatattta tgatgccgat atctttgaag
     5461 aattatctag taatgaacag caacaagtca gaacttggaa aacagataat tcagcagaag
     5521 caatggacaa gctgtatcaa ttgattttta taaaaatctg taatcaattg aatgattact
     5581 tacctgagct atttgaagaa gtagaagatt atacagaact tttgtttaca gcttcttata
     5641 ttaaaaaaga tggtgtaatc gctgatcttt taagtattcc agaggatgat ttctatgttc
     5701 aagctggtgg acaggtagag attattgggt ggttgtatca gtactataat agtgagccac
     5761 ataatcaagt ggtgaatatt tataaaggga ttgtcaaaaa acaagatatt ccggcagcaa
     5821 ctcaactatt taccacagat tgggtcgttc gttatatggt tgacaatagt ttaggaaagt
     5881 attggttaga tcattatccc aatagtgaat taagaaataa gttgaaatac ttaattccag
     5941 ataaatatgc ttttgaacaa gcaacatctg taagtaattt aaaaccagaa gaactaaaaa
     6001 ttatcgataa cgcaatgggg tctgggcata ttttagttta cgccttcgat gtattgatgg
     6061 aaatttatct tacgttagga tattctgagc gtgatgctgc acgtgcaatc gttgaataca
     6121 atttatatgg tcttgaaatc gataaaagag cgtatcaatt gtcttatttt gcaattatga
     6181 tgaaagggcg tcaatacgat cgtctttttt tgaaaaaaag tctatcgccg aatttaagag
     6241 agtttattga ttctcctaaa gtgacagacg agtatcaaga gcgtattagt gaattgaaca
     6301 atgataagta tagtgaacgc tggaatgaat acaaagagtt aatttttgat caatttgaaa
     6361 atgggaaaga acttggatca atcattaagt tagtcgataa aaataatcag gcaataacta
     6421 aagaatactt aggaaaagta agagagtata tatcaaaatt caacgaattt acgaatatgg
     6481 atattcttta tgggatgaag gaaatacaag aaaaatactt gcacattctt gacgtggctg
     6541 aaatgctagt tgataaatat gtagcggtag tcactaatcc gccttatctt aataaaatgg
     6601 ataagaaatt aaaaaaatat gtaaatgata actattctga tgtcaaaaaa gatctatttt
     6661 ctgtgtttat taaattgaat agccaaatgc tgattgataa tggatatgct tcctttatga
     6721 cgccatttgt ttggatgttc atttctagct atgaaaagtt acgctcattt ttaattaatg
     6781 aaagaaacat ttctagtttg attcaaatgg agtattcagc ttttgaagaa gcaaccgttc
     6841 caatttgtac gtttacgata aaaaattcaa atgaacaaga tggaagctat ataaaattat
     6901 cagattttaa aggtggaatg gaagttcaaa aaattaaaac gttagaagca attcatcagc
     6961 atggtgaatg cgattatttt tatagtacta atcaagacaa cttcaaaaaa atcccaggaa
     7021 tgccgattgc ttactgggca agcgataacg taatcactga ttttgctcaa ggaaaattaa
     7081 ctaacgatgt tgcagatgca aaacaagggt tggcaaccgg aaataacaat aaatttttgc
     7141 ggtattggtt tgaagtagaa tttaacaaaa taaagtttga tgctaaaaca ttagaggacg
     7201 ctaaaaagtc tagaaaaaaa tggtttccta caactaaagg tggtcgtttt agaaaatggt
     7261 atggaaacaa tgaatacgtg gttaactggg agaatgatgg agatgaaata aaaaattatg
     7321 ttaatgaaaa tgggaagtta aaatctagac cacaaaatct taattactat tttaaaaagg
     7381 gaattacttg gtcgacactt tcatctggac aagtatcttt tagattgcag aatcaggcaa
     7441 tttttgaaac taaaggatct gttctgttct caaaggatat aactgacttt gaagaaatat
     7501 ttggctatgt aaactctaaa ttagttaatc actttttaac ttttatttct cccaccttgg
     7561 attaccatga aggaccaatg ggaaaactac cttttcttaa agttacatct gagagaaaat
     7621 taaaaaaaat aagagaaaaa gtggatgaaa atatcgatat agctagaaaa gactataatg
     7681 ctcttgaaac gtcatacgag tttagaagaa acattttgtt aagttatatg tttaaaaaag
     7741 atctctcaga ggcatttaac tgtctaagta ggcagttggt taatcaattt aatactctaa
     7801 aatcgaatga agaggaactg aacgacatat tcattaatca ttattctctt gaaaaagaat
     7861 taacaaaaga ggttcaagat gaatatatct ctgcaaggtt acctgataag gcgagggaaa
     7921 ttaaggattt tatttcttat tcaataggtt gtatcttcgg tcgttactcc ctagacgaag
     7981 atggtctcat cttcgctggc ggcgaatggg atccttcccg ctatcaaaaa tttaaacctg
     8041 atactgataa tgttttactc atcaccgatg atgagtattt caaggatagc caaattgaca
     8101 ttgtcaatcg tttcattgat ttcgttgaga ttacttttgg taaagaaacc cttgaagaaa
     8161 atctccagtt tatcgctgac actttaggtg gcaaagggac aagtcgagaa attattcgtc
     8221 gttacttcgt acaagatttt tacaaagacc atttaaaaac atactccaat cgaccaattt
     8281 attggcaatt ggactcagga aaacaaaatg gctttaaagc gttaatgtac ttgcatcgct
     8341 atacaccaga tcaattagga aaagtacgta ctgaatacct acacgaacta caaaaagcgt
     8401 atgacaatcg aatcgaattg cgtcagcaac aaatttcaac aagtgataac ccaaaagaaa
     8461 gaaatgcaat gacaaaagaa attgtaaaga ttcagaagca attaaaagaa gttcgtgagt
     8521 atgacgagaa gttgggtcac ttagcattaa aacgaattga attagactta gatgatggtg
     8581 taattgtcaa ttatgataag ttgcagcgtg atcctaaaac cggcgagaaa ttccaaattt
     8641 ttgcaaaagg ggtcaaagaa ccaaaaaaat agttatctaa gaaagttgtt ttctaacaac
     8701 tttctttttt attcttaaat tgcgtgcgaa attatccaaa ttatgtcaat ataaggggta
     8761 tagaaagaga acgattaggt gagattgatg acagatataa atacgcaaca aattgaagaa
     8821 aaattaaatc aacgttttcc taattatgga tgtaggaaaa tcattttttg gtttgattca
     8881 aatcaggatt ttcttgaaga aattgactca ttggtcattc aaaatgcaaa aatctatcga
     8941 ttagaaaagg acgcacaatt taaaactaag cgtttattgg aatatgaaga tcccgaaact
     9001 aactatttaa tctatgcgcc atttcctcgt tgggatgata aagatgaaga taatcatttg
     9061 ttgagtgtat tgaattattc agaggaattt agtgcagacc gcttagcgat tatcatgaat
     9121 gaattggcta ttccacttag atttcatcag acagttaaac agtatcgtaa tttctttaat
     9181 gcaaaggatc gcatgagaag ttttggtgaa tttagtacgc atatgacaat taataaatca
     9241 gaaacgattg aattaatgat aatggctgtt ttgttgaaat cgaaaagtat tcgattaaat
     9301 gatttactgc gtgtagttct aaaaagcttt acagaaagta gggagaatcc tttaagggaa
     9361 cttgataagt atcagttaac agctaaattt tgggaattta ttcaaatgaa atttggcttt
     9421 acggatagcc agccaacatt ggaaaagctg acgatgtgtc tttatttgaa ctatttttat
     9481 tatcaaattg atcaagagat gccgaagaag ttcaaaggat ttgaaatact acatttgaaa
     9541 gcaaacatta ttactttcat ggatcagttt atgaatgata cacgttacat ggaaagcttt
     9601 gaacagctat ctaaaaaagt atatcaggct attgatggtg atggattatt agacagtata
     9661 gaaattgatg atctaattga agcagacgta ttttctgaga ttcatgcaaa aatattaatg
     9721 ttttatgcag aacgattaaa ggcttctgac actacaacga aaattgcagg acaaacaatt
     9781 tttgatagtt taaagcagcg cgaaaaaatg catttcgcat cggattatat ttatgagtat
     9841 caattattga aacatgctta ttatcttgtg gataaaaaca gattgtgctt gccgatgaac
     9901 atggataatt tgctaactca atatgagaag gaaatgtatt gggttgatac gcattatcgg
     9961 aaatttattt ggaatagtga tcatatagaa gaaaagcaaa aatatggcca attgtatcga
    10021 ttagttgaag caaattattc gaagtttctt gatcaatcag gacaaatttg gaatcaaact
    10081 tttgaattta agaatcgtcc atccattcga aaattttata atcaagctgt aatgaaacgc
    10141 tctgttaaaa ctgtagttat tatttcagat gcttttcgat atgagttagg gatgaattta
    10201 gaacgtaagt tagttagaga aaagaagtat tcaacaaaca tggctagctt tctttctgta
    10261 ttaccttcag ttactgaatt cggtaaagcc gccttattac caaacaatca actaacttac
    10321 aatgaaggaa cagaagtact gattgatggg aaaaaaacga atggtttatc caatcgaaat
    10381 gcgattctcc agcaacgaga tgcaaatgct gtagctttaa attatgatga tgtggttaag
    10441 atgaataagg ctgaggtacg agaagtattt aatggcaaat cagtcattta tatttatcac
    10501 gatcaagtgg atagaacagg tgatcactcg aatgaaaatc gagtttttga ggcctgtgat
    10561 ttagcgataa gcgaaatttc ccgacttatt aatcgcttgc ataatgatgg cagtatctat
    10621 cgttttatag ttacggctga tcatggattt atttataaaa gaagccacgt tgaagaagta
    10681 gataagattg aaaatccatc tagaaatgaa aaagatcgtg tagaacgtcg ttttattctt
    10741 agtgaagaag aatacgaaat aattggtacg aaaaattact ctttgggaga ttctttagga
    10801 aatgatgaca ctagaaacat ttgtgtcccg attacttcaa gtatttttaa gaaagctggc
    10861 gggggacaaa gctacgttca tggagggtca tctattcaag aaatgctagt ccctgttcta
    10921 gaaattacgg tagccaaagg tagcaatgca aaggaaccag tagcaattga attaatgact
    10981 agcaaccgca gaattacagg attatcaacc acgttagaat tctatcaaaa aaatgcaatt
    11041 agtgatgttt atgaccccgc atctttctgg ctttattttg aagatgaata tggtgaacgt
    11101 atctcaaatg aagaaatcta tgttgcggat tcaaaaacag aagatgcaaa tcagcggttt
    11161 actaaatttg tttttgaatt tgtaaatcgt cgatatacag ttga
//
LOCUS       DAZKLA010000097        11198 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000097 DAZKLA010000000
VERSION     DAZKLA010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.098"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACG85N_002134"
     CDS             complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACG85N_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFQ0224861.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACG85N_002135"
     CDS             complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACG85N_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFQ0224862.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(1681..2782)
                     /gene="prfB"
                     /locus_tag="ACG85N_002136"
     CDS             complement(join(1681..2709,2711..2782))
                     /gene="prfB"
                     /locus_tag="ACG85N_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFQ0224863.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACG85N_002137"
     CDS             complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACG85N_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFQ0224864.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="ACG85N_002138"
     CDS             complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="ACG85N_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFQ0224865.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(6343..7062)
                     /locus_tag="ACG85N_002139"
     CDS             complement(6343..7062)
                     /locus_tag="ACG85N_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFQ0224866.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(7059..8372)
                     /locus_tag="ACG85N_002140"
     CDS             complement(7059..8372)
                     /locus_tag="ACG85N_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0224867.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(8453..8773)
                     /locus_tag="ACG85N_002141"
     CDS             complement(8453..8773)
                     /locus_tag="ACG85N_002141"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0224868.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(8881..9168)
                     /locus_tag="ACG85N_002142"
     CDS             complement(8881..9168)
                     /locus_tag="ACG85N_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224869.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            9295..9939
                     /locus_tag="ACG85N_002143"
     CDS             9295..9939
                     /locus_tag="ACG85N_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFQ0224870.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(9998..10705)
                     /locus_tag="ACG85N_002144"
     CDS             complement(9998..10705)
                     /locus_tag="ACG85N_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0224871.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(10925..11095)
                     /locus_tag="ACG85N_002145"
     CDS             complement(10925..11095)
                     /locus_tag="ACG85N_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224872.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3053 a         2430 c         1957 g         3758 t
ORIGIN      
        1 tatattgtgg tattagaatt tcaaaaaacg tctcatagaa atgatcgagc caagtgaacc
       61 aatgatcaca ccaatgccga ccattaaaag attgattttc caaacgatat tttgtggacg
      121 aatcaatgaa taatgggaac gtaataatga tggattgatc aattggaaaa accatgcata
      181 accaaatgtg atgatcaaaa tcggaagaat cgcaccgatc agaccgatcc agccaccttc
      241 aaggaaaaac ggccagcgaa tatatccatt ttttgcaccg actaaacgca tgatttgaat
      301 ttcacgctgt ctagacaaaa tcgtgatccg aatcgtgttc gaaatgagaa acatcgctac
      361 gaacagcagc aatgccgctg caactaatcc ccatgtccga actgcaccag cgatttggaa
      421 aatccgatct gaggaagctc caccgtaatc agaacgagaa acattcttca aatctgctgc
      481 ttcttctgct acttgttttg tataagaagg atcagtagca cttactacat acacatcata
      541 taacgggtta ttgtcacctt caaacaattt ccaggaatca cccatttcgt tttggatttt
      601 tttcaattcc tgttctttgc ttgaaaattc aactttttta acatgatcta agtcattcaa
      661 ttctttttct agctttttca tctcttcgtc ttttgtaccg atatctacga atacagatac
      721 atcaacgttt ccttctatat cctgagctaa tttagttgca ttcatgataa ctgccatgaa
      781 tacaccgact aaagtcaacg tgatcgttac cgcactgact gaagcaactg tcatccagcc
      841 gttacgtttc aaactcttga tactttctaa taaatggcgg aaaaaggttc taatcatcgt
      901 atccatattc cccttccgct tgatctcgaa taatgcggcc gttttcgata gcaattactc
      961 ggtgccgaat cgtatttacg atcgtactgt tatgtgtagc catcacgaca gtagttcctt
     1021 gggcattgat ccgatctaat aatttcatga tttcccatga gttttctgga tccagatttc
     1081 ctgtcggttc atccgcgata agtactttag gcgtattcac gattgcccga gcaatagaga
     1141 cacgttgctg ttccccaccg gataattcgc taggaaagac tcgaactttg tgtttcaaac
     1201 caacaaggtc cagcacttcc attactcgtt ttttgatatc tcgaggtttt ctgccgataa
     1261 cttgcatagc gtacgcaaca ttttcatata ctgtttttct tggtaataat ttatagtctt
     1321 ggaacaccac gccgatttct ctgcgtagat aaggaacttc gcggtttttg attgctatta
     1381 gatcatgacc tgcgacgttc aaagttcctt ttgaagcttt ttcttctcga tacatcaatt
     1441 tgatgaatgt agatttaccg gctccagacg gaccaaccac atagacaaac tctccttgtt
     1501 cgatgacaat cgagagattg cggatagcgg tcgtaccatt tgagtacttc ttcattacat
     1561 ccttcatttc aatcatggcg ttctctccat atctttttca gtctttagct attatagcat
     1621 ggcaatgttt caacatgctt gcaaaatctt acaattttat ttcaatttaa caatgagttc
     1681 ttattgtaga tgcattttta aatagccgtc aataaataaa tcaaggtcac cgtccataac
     1741 tgcttggaca tttcctgtct ctacgtttgt ccgatggtct tttaccatag aatagggatg
     1801 gaaaacataa gaacggatct gtgaacccca gccgatttct ttttgttctc ctcgaagtgc
     1861 agctgcttct tgttctttct tttccacttc taattgataa agcttagctt ttaacatccc
     1921 catcgcttgc tcacggtttt tcaattgtga gcgttgtgcc tgactagcta ctactgttcc
     1981 tgtaggcaag tgagtgatcc gtacagcaga ttctgtttta ttgatgtgct gaccacctgc
     2041 accacttgct cggtacgtat cgattttcaa atcatctgga ttgatttcta tatcgatcgt
     2101 ttcatcaagc tctggcatca catctaccga acaaaaagaa gtatgacgtc gtttcgctga
     2161 gtcaaaaggc gaaatccgta caaggcgatg aacacctttt tctgatttta gataaccaaa
     2221 agcgttatgt cctttaatca gcagggtcac actcttgatc cctgcttcat cacctgcttg
     2281 ataatctagt gtttctactt gatagccatg ctgctctgcc caacgtgtat acatacgtaa
     2341 aagcatgctg ccccagtctt gggactccgt tccgccagcc cctggatgca attcgacgat
     2401 cgcattgttt cgatcataag gtccgtctaa cagcatagat aattcatacg tcttcatttt
     2461 ttcatctaat gcattgatac gtgtttctgc ttcttcttgc attgctgcat caggttcttc
     2521 ttgcagcatc tccaccatca attcgatttc ttctagttct tcggcaagag aatggaattg
     2581 gtcataggtt tctttattag cgttattttc gctgatcact ttttgtgcgg cttcgctgtc
     2641 atcccaaaaa ccaggttcag ccatttttgc ttctgcttcc gcgatgtctt cttccagctg
     2701 atctaagtca aagagacctc ctgaaacttg tgaccttttc ctgcatttct gtcaggagat
     2761 ttcgaatttc ggatatttcc atgttttttc cttctttcac tatacttttt tcattatatc
     2821 tagtctaaca gaatcagcaa ataacagaaa gaaggtgaga cgaattctta aaaaaagaat
     2881 ttgtctcacc aatcctgctg tcagaaatta gtttgcattt tttccatgac agttcttata
     2941 ttttttcccg cttccacaag gacaaggatc atttcttccg accttgttca catggattgg
     3001 ctgttttttc gcgctagtat tactattttc tttggcatct ccgtcttctg agtgagaagc
     3061 tgttccttga gaaacctgtt cacgctgtac gttttgacgg atctcagctt tcataaagat
     3121 tcgagtgact tcatactcga tggcgcctac catatcttca aacatcttat agccttctgt
     3181 ttgatattcg actaatggat tgttctgtcc ataagcacgt aagccgattg actgacgtaa
     3241 ttgatccatt gcatcaatat ggtctgtcca tttagagtcc acaacacgca agatcacgac
     3301 tttttcaaat tccaacagtt gctctggacc atttagttga gccgctttgg tattaaatac
     3361 ttcttgtgct cgttcgttta gatactcttt gatctcctga gatgtttttc cttcgatatc
     3421 tgcaagagta atactatctt catggacaag agtactgccg gcaaaatcga tgatactttc
     3481 caaattccaa ttttctttct ctaattgtgt atgcgcatct actacacgac tgattgtccg
     3541 tttgaccatg ttcattagtg gcttggataa gcttttttct tccatgatta cttcttgtcg
     3601 ctgtttatag attacttcac gttgttcacg catcacatca tcgtattgta agacgttttt
     3661 acgtgtgtca tagttatttc cttctacacg tttttgtgca gattctactt gtttcgtcag
     3721 catcttgctt tggataacag catcttcctc accgatcttc atccgatcta agaaagcttt
     3781 gattcgttct gaaccgaatc gtttcatcaa atcatcttcc aatgacaagt agaattgtga
     3841 cataccaggg tctccttgac gtcctgcacg tcctcgaagc tggttatcga tacgacgaga
     3901 ttcatgtcgt tctgtcccga taactgctag accaccaagt tctcttaccc caagtcctaa
     3961 cttgatatct gttccccgac cagccatgtt ggtagcaatc gtcacagcac ctttttgacc
     4021 agcattcatg atgatctctg cttctttaaa gtgatttttc gcattcagca cttcatgagg
     4081 aatacgttct tgattcagca tatctgataa taactcagat gtttcaaccg caacagttcc
     4141 gactaacaca ggttgacctt tgcggtagcg atctttgata tcttgcacga cagcgtggaa
     4201 cttgctttgt aatgttgggt aaagtaaatc tggacgatca tcacggataa ccggacgatt
     4261 tgtcggaatc tggtatactt ggatgttata aatttctctg aattcttctt cttctgtttt
     4321 agctgtacca gtcattcccg caagtttctt gtacatacgg aaatagttct ggaaagtgat
     4381 cgtcgccatg gttttcgttt cgtcttcgat ctctacgcct tctttagctt cgattgcttg
     4441 gtgaagtcca tctgaataac ggcgtccatc catgatccga ccagtgaact ggtcaacaat
     4501 cagtactttt ccgtcttgga caacataatc gatatctaaa agcatgatat agttggctct
     4561 caatgcttga tccaagtgat gggtcaatgc agtattttca atgtcgtata agttatccaa
     4621 accgaatgtt tcttctgctt tttcgatccc tgcttcggtt aaactgattg tttttgattg
     4681 tacatcgatt ttataatctt cttcttcttt caaacgtttc acgaagttgt ctgctcttgt
     4741 atataaagct gtggattttt ctgcttgccc tgaaatgatc agcggcgttc tcgcttcatc
     4801 aatcaagatt gagtcaactt catcgacaat ggcataattc agcggacgtt gtaccatttg
     4861 atggcggtaa acgaccatat tgtctctcaa gtagtcaaat cctaattcat tattcgtact
     4921 ataagtgata tcacaattat aagcctcgcg tttttcatcc gaagattttg agttgatatt
     4981 caaccccact gttaatccta agaaattata aagttcgccc atttccgtcg agtcacgggt
     5041 tgctaaatat tcattgacgg taactacgtg taccccttcg ccagtcagag catttagata
     5101 tactggcatc gttgcggtca aggttttacc ttcaccagta cgcatttcag ggatatttcc
     5161 atcgtgaagc acgattccac ccatcaattg tacgtggtat ggatataacc ccaatacacg
     5221 ttttgctgct tctcggacga ctgcaaaagc ttccggaagt aattgatcta atgtttctcc
     5281 tttttggtat ctgcccttga attcagcagt ttttgcctgt aattgttcat cagttaatgc
     5341 tgccatttgg tcagcgtatg cttcaacttt ttttgcaatg ctatctaaac gttttagttc
     5401 tttcttatcg ttttcgataa gtgaacgaat caaattggcc atgcgttgtg taatctcctt
     5461 ttccttctgt gtattatcca gactatgtag actttacttt tcattttatc attagttata
     5521 gataattgaa atatcttaag cgtattaaag taagaacttc ataatttatt catatttcat
     5581 ctaaaagagg aggaaactca aaaaaacttg cggcagctgt cagtagctgc tgcaagtttt
     5641 cgttgttatt taagtaaggc tgatcagtct gtttcaatca gtccgtattt tccatcttta
     5701 cggcggtaaa cgatacttgt accatttgtt tctgcatctt caaaaataaa gaagttatgt
     5761 cccagcatgt tcatttgtaa gactgcttct tcactatcca ttggtttcaa tgaaaggcgt
     5821 ttcgtacgta cgatatctaa ttcagaatca ttttcgtttt cgtcctcttc attgaataaa
     5881 gcagcagcat ctgcagtatt catgcccgtt tcacgggatt tacgattaat ttttgttttg
     5941 aatttgcgga tttggcgttc caatttatca acaaccaaat cgatacttgc gtataaatct
     6001 ggagaagttt cttcggcacg taatacaaga taaggtaatg ggatcgttac ttccactttt
     6061 gctgtttttt ctgtataaac ttttaagttc acatgcgccg ttgcatcggg tgcgtcactg
     6121 aaatagcgtt ctagtttgcc tacttttttc tcaacgtagt cgcgaatcgc ttcagttact
     6181 tcgatatttt cgccgcgtac attatatcta aacataaaaa ttaccccttt cgtctaccac
     6241 aatcaagaag gagaaggacc actctttccc tcttcttaat tctattatat cgaactctag
     6301 gttatttgca aattaaatcg ctatcaaatt tccacatcta attcatctcg ccaaagaaat
     6361 ggttcggatg attttcggct gtgcgacctg caaacaatct gctgcgtgga aaagagtctg
     6421 tcctgttgta tagacatcat caataatcaa tattttttta ttttgtatct tagaaaaggt
     6481 gactgcaagt tcaaaaggtt gaggcatctc caatctttcc tttcgcgtgt tttttgcttg
     6541 agccagcaga tgcttctctc ttttgagtag catctgaaaa ggaattcttg ctgctttcaa
     6601 catctcgctt acttgattga atcctctttc tgttcgcctt ttttcagata aaggtattgg
     6661 acaatagatg tatgtcgggt attctttttt tatctgcgtc catttatatg caaaggtgcc
     6721 tgctagtcgg atatctccca taaacttgta ttgattgagc caggccttca tattttcatc
     6781 gtacacaaaa aaggcacggt gctgaaaatc ataggtcgga tatttcttct tccattcttg
     6841 acagtcactg cagtaaacgg gaatagactt tactttcttt ttcgtactct ctagattgaa
     6901 atggattggt ttttgacagc cagaacagcc tcttgtttcc aataaatgaa attgtgcgcg
     6961 acaaactgaa caagtctgag gtaatctgta tttcgaaaca ccaataatct cgctgaatgt
     7021 taaattttgg aagatatctt tactgcagca actgcacctc acagcatctt cttcccttct
     7081 tcattcattt ctttgatttc ctgacaagct tttcgaatag ctgcagtttt ttcttcatag
     7141 aaaaagtata cttgactgtt cgtataaggc cctttacgat cggctcttcc tgcgatttgt
     7201 accaaagaag atttagtaaa aaccggatga tccgctccta aaacgataac ggatattctt
     7261 tcaaatgtga ctccacgttc aaggatcatc gtggttaaca gtatatcata ttttgcttct
     7321 cgcattttct ggactttttc cgcccgctga ttgtctcttg aagaaacatc tgtgatctca
     7381 tatctcggaa gagcctcttg aagtgtggct ttcaaacgat gcatcatcgt gatacttgga
     7441 caaaataaaa ggatatggtt ttctttaatt aattgatgaa tgatccgcac caaagatttt
     7501 atcttcttct tgttcttgca tttttgtcgc caattattca tgaaaaccaa gacgggttca
     7561 ggaagcaaac gccgatgaaa gcgtaaagca agtttgtgga tgtcaaacgt ctgcttgatt
     7621 tcctttagta actttttatc aggtgtggca cttaagtaga tgaatcgtcc gtctggttta
     7681 atcgctgttt gcacagcatg agctaatcct gcatccccgt tataaggaaa cgcatcgatc
     7741 tcatcgatca ctattaaatc aaaggaccga taaaaacggt atagctgatg aatcgtacag
     7801 ataacaaaag aagtaaaggt ataagccgtt gctgtattcc catggagcaa cgtgattttt
     7861 tcttctggaa aaacataacg atagcgtaaa aacaattcat tacaaacatc gactcgcggt
     7921 gtacaaatcg ctactcttcc tccagctttt aaagtttcca ctaaaatagg ataaagcatc
     7981 tctgttttcc ctgcacctgt tacagcccaa ataagatgat gtcttttttc tgaatggcaa
     8041 agtattctct ttgagatttc cgtttgtttt tcagtaagct gtcctgtcca aaaaaagacg
     8101 acttctcgag gttttgcttc aggctgatca aacaaatata aatcttgttg agtgtcgcat
     8161 cgccctaaat atagacaatg tgtacaaaaa taaccgtaat ctgcaagctg tccatcaatg
     8221 acggacgtcc tcattccaca gcgatgacaa agaatccagt tgcccaaacg ttccactgct
     8281 gcgatcttct cataatttgg taaatttacc tcctcacagt ctattgcttc aatatctctc
     8341 gtcagaagcc ttctacccca aaaatgattc atattcttcg ccctaaaaaa atatacgaaa
     8401 aaacaagcga tctatttttt ggatcgcttg ttcgatagtt atactgttct atttattcac
     8461 ctatgagcgt gattgcttga tctaacactt tttcgccatt catcatacca taatcagcca
     8521 tattgatgac atctaaagga atgccttttg gttccagacg tttctcaaaa tcagctttca
     8581 taaagcgaac ttgtgggcct aataacaaca catccacgtc tttatttgca aggttgttat
     8641 ctgcgtcaga agcggacaca gcgaaaatat ccgagtcgat tccttttgct tcagcagctt
     8701 tttgcatttt tgttactagt aaacttgtgc tcataccagc cgaacataca agcatgattg
     8761 tttttttagc catacttttc tctccttttt aaaacaaact atgcaaacgt tttcttcgcg
     8821 tctgttactc tattatataa aaagaaagaa atttgtcaac gttttctaaa cgcggtttat
     8881 ttaatcaaat agccaactag atttcactaa tttattgaac acgacatatt ctttgtccaa
     8941 attgatgaca actacttcat agcctgcatt tatccagcca taggcacctt tttcagggtg
     9001 tttataatca tttgcccacc aatctgtact ttcccttgct gttttgggaa gtccgttact
     9061 gggaaacagc agttcgtcaa attgggtaaa ggtcaacgtt acttgagagc cgccattgtt
     9121 cttcaaataa tgcgtaattg catcgtactt tttcattcgt tcctgcatcc ccagatccac
     9181 ctgcctttgt ctttattatc gcatactaca gcaattgcgc aaccgttaca gtgttgaaac
     9241 aacctagaac tttatttata ctgaataaag aaaatgaaaa aaagaggtga caagatgatc
     9301 gagcgctatc gtacaataaa agaaaatgga caaagtgaga ttgagatcaa aaaatcacgc
     9361 tttatctgtt ctcttagacg aatagaatca gaggaccaag caaaaagttt cattcaagaa
     9421 ataaaaaaag aacattggaa agcgaaccat aactgcagcg ccttcatcat tggagaaaaa
     9481 aatgaaatcc agcgaagcag cgatgatgga gaaccaagtg gaacggcggg cgtaccgatg
     9541 ttggaagtcc tcaaaaaaaa tcaactgatc aatgtagctg cagtagtcac tcgatatttt
     9601 ggaggaacga aactcggcac aggcggatta atacgagcat acactcatgc ggtctctcaa
     9661 gcattgtctg acattggaat cgtagaaggt accttgcaac aggagatctc tgtgatgatc
     9721 acgtatccgc aactaggaaa attacagaat tatcttgaac tccaacattt ttttataaaa
     9781 gaaatccaat atactgatca agttagcgtc atctgtgctg tagatgaaaa aagtgctgac
     9841 cactttaaag aatccatcat tgagctgctg aacggacaag tccaatttga agagggttct
     9901 gtttcttatc acgagcaact gataactgat catatataaa aacaggtgat tctgattctc
     9961 ttttatttct aataaggaat cagggatcac ctgtttttta tttttccaaa taaaactcaa
    10021 aacgatttcc tacgtattgc gtacgcacat actcaaacgg cgtaccgtct tcaaaataag
    10081 aaatctggcg caaacgaaga actgcatctc ctcgttttac ctctaaatag tcagcaattt
    10141 tctcagaagc caacacagca gtaatcgttt gattcgctgg accgatcttt ttcccgcctt
    10201 tttcttctag cgtgcgatac aacgattcag taatctctgc tttgctgtag ccatcgatca
    10261 gcttttgtgg aacactggca acttcaaaac aaatagggac atcatctgcg aaacgaatac
    10321 gttccattcg caagatcgtg tcatctttac tcaagtttag tttttccatt tcacttgagc
    10381 ttggcgaagt caagaaataa gagattgtcc gactagacgg tactcgattt tgtgattcca
    10441 taatttctgt aaaacttgtt gcaccagaca ttttttcttg tactttttct cgcgcaacat
    10501 acgtacccga accgatcttt ctttctaaga ttccttcatc tgccaagttt tgaattgctt
    10561 gtctcaacgt catacggctg acattgaatt tcactgctaa ttctctttct gaaggaagac
    10621 ggtctccgac tttccatata ccctgttcga tttcattttt taattggtca tgaatctgta
    10681 tatatacagg taattgattc gacataacgt acacccgtcc tttcgtttct tctattataa
    10741 ctggtatata cctgattcac aactgaaaaa gataaaggtt tctatccatc aacagaggat
    10801 ttagacacac atcgttctcc ctactcctat tgtcagaaac aattttttag atatgttcaa
    10861 cctagaaaat aacaaaagac cctaaaatcg atgaaaatgc cgacttatgg atcttttgtt
    10921 attattagcg gtttttagcc atccattttt ttgtatgatt cgttgggcgt tttgatttta
    10981 cctggaccgc ttttccgcct tttttcggaa tattcacttc aatattgtat ttttctgatt
    11041 tgaccaaatc atataacttt tcagctgctt cgtctacttt ttctttccat ttcatgtgga
    11101 tccctccaat tcttgtagtt aacttaactc tatcttaatt cagcagaatc ttcaaggaac
    11161 aaggtagcaa agcaaaaaaa agagctctcg aactaacg
//
LOCUS       DAZKLA010000098        10787 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000098 DAZKLA010000000
VERSION     DAZKLA010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.099"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..1250
                     /gene="mltG"
                     /locus_tag="ACG85N_002146"
     CDS             75..1250
                     /gene="mltG"
                     /locus_tag="ACG85N_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFQ0224873.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            1386..1865
                     /gene="greA"
                     /locus_tag="ACG85N_002147"
     CDS             1386..1865
                     /gene="greA"
                     /locus_tag="ACG85N_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFQ0224874.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            2034..2765
                     /gene="liaF"
                     /locus_tag="ACG85N_002148"
     CDS             2034..2765
                     /gene="liaF"
                     /locus_tag="ACG85N_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFQ0224875.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            2762..3829
                     /gene="liaS"
                     /locus_tag="ACG85N_002149"
     CDS             2762..3829
                     /gene="liaS"
                     /locus_tag="ACG85N_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFQ0224876.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            3836..4468
                     /locus_tag="ACG85N_002150"
     CDS             3836..4468
                     /locus_tag="ACG85N_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0224877.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            4608..5273
                     /locus_tag="ACG85N_002151"
     CDS             4608..5273
                     /locus_tag="ACG85N_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFQ0224878.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            5301..5594
                     /locus_tag="ACG85N_002152"
     CDS             5301..5594
                     /locus_tag="ACG85N_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFQ0224879.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(5662..6609)
                     /locus_tag="ACG85N_002153"
     CDS             complement(5662..6609)
                     /locus_tag="ACG85N_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0224880.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(6787..7590)
                     /locus_tag="ACG85N_002154"
     CDS             complement(6787..7590)
                     /locus_tag="ACG85N_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFQ0224881.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            7736..8650
                     /locus_tag="ACG85N_002155"
     CDS             7736..8650
                     /locus_tag="ACG85N_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0224882.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            8698..9168
                     /locus_tag="ACG85N_002156"
     CDS             8698..9168
                     /locus_tag="ACG85N_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFQ0224883.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            9253..9861
                     /locus_tag="ACG85N_002157"
     CDS             9253..9861
                     /locus_tag="ACG85N_002157"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFQ0224884.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            9995..10195
                     /locus_tag="ACG85N_002158"
     CDS             9995..10195
                     /locus_tag="ACG85N_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0224885.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         3679 a         1649 c         2209 g         3250 t
ORIGIN      
        1 aaaatcattc acgccaattc aaatatcatg tataatgaat aaagttacga acttgatagt
       61 aaggaggagt tgttttgtca ggaaacaaac aacacccaag taaagataaa aaagaagcca
      121 gtaaggcagc tacagcagat ataatcgaca agcggagaaa tctgcgtaaa aaagaagata
      181 agattgtgag gaagatcatt ttagtgattg ctcttacatt attgattatt ggcggatttc
      241 ttggatttac cgtgtatcgt tatgtagata gcgggttgaa gcctttagat aaatcagatg
      301 accaattagt ccaagtcgag atcccgagtg gctcttctaa caagcagatt ggtgagattt
      361 tggagaagga caatattatt aaaagcggga ttgttttcaa ttattataca aaatttaaaa
      421 atttaactgg ctttcaagct ggctattatc agttagcgcc taatatgact cttgacgaga
      481 ttggcaaaca gttgcaagaa ggcggtactt ctgagccaac aaaagttgca gatgggaaaa
      541 ttgccattcc agaagggtac gatatcgatc agattgctga acgggtagcg aaggtaactg
      601 gtaaagacaa gaaagaattt ttagatttag tgaatgatga gactttcttt aacgagttgc
      661 accaaaaatt cccagaactg ttggaaagtg catctaaagc tgagaacaca aaataccgtt
      721 tggaaggtta cttattccca gccacatatg attattacac gagtacttcg cttaaggatc
      781 tagtgattga aatggtgaac aagacgaata cggtcatgca aaactattat tcagctatca
      841 agcagaaaaa tttgactgta caagaagttt taactctagc ttctctagtt gaaaaagaag
      901 gcgtaaaaga aaatgatcgt aaaaacattg cacaagtctt ctttaaccga atcaaagcaa
      961 atatgccact tcagtcagat atctctgttt tatacgcatt aggtgaacac aaagaattag
     1021 tgacgtatga agatacagct gtagattcac catataattt gtataccaat accggctatg
     1081 gacctggacc ttttgacaat ccaagcgaag aggcaatcaa ggcagtatta gaaccagcag
     1141 aaaatgacta ttattatttc gtagcagata cgagcactgg aaatgtttat ttcgctaaaa
     1201 cttatgaaga acatatggaa ttagtggaaa aatatgtaaa taattcttag aaaaacaggc
     1261 tctagacatt gatcattttt agaaaaacat ttacatttga agaaaagcgt ggtaatcttt
     1321 tttggactat attcctcaaa agattacctt ttgcggtatt ttatcgaaaa aaggagacgt
     1381 tacacatggt agagaaagtt tatccaatga ctctagaagg taaagaaaaa ttagaaaaag
     1441 aattagaaga actaaaaaca gtcaaacgcg gagaaatcat tgaacgaatc aaaattgccc
     1501 gcggtttcgg agatctttcc gaaaactctg aatatgaatc agcgaaagat gaacaagcat
     1561 ttgttgaagg aagaatcaca acaattgaaa atatgattcg ttttgctcaa atcatcgata
     1621 acgacggtgt agatgcagat gaggtatcaa ttggtaaaac agttacattc atcgaactac
     1681 cagatggaga agaagaagag tacacgattg tcggaagcgc tgaagcagat ccgttttctg
     1741 gcaaaatttc caatgattct cctattgcgc aagcattgat tggcaaacgt aaaaatgatg
     1801 aagtaactat tgctactcca ggtggcgata tgttagttaa aatcgtcaaa gtagaacaag
     1861 cgtagtctga ctgaaagatc tagagagttc atcttactga tgatacactc tagatctttt
     1921 tctctttgtg tattttagat ttcaacaaac gatcttacat ctaaagtcct atagccaaca
     1981 gttaaaaaaa taggtatgat gaaatgagaa tgtgtcagga gggagaaaga gcgatgaata
     2041 gttcttggcg tttttttgtg gtagttgaag cactactgct gttatttgct gtttggcaaa
     2101 tcgtaaacaa tactggactt ttgcttttag tgatattcgg tattttcaat atatatctag
     2161 ctatgcgcaa acatcctcgt actaaatttc aaaattttca attgatatta ggaagtttgg
     2221 tcattttttt tagcttagtc aacagtcctg ctttatggat gatggctgta tttgccgttt
     2281 tatttattgg tctgaaaggt gtagagattt cagggataga tttaaccaaa aatgcttttt
     2341 ggcgaaagaa acagatcatg atgatccaaa cagagcaatt aaaaacacat aataatgagc
     2401 ggaaaagaca gcaattattc ggtaaccaac gtattggaaa cgacgtctat gaatgggacg
     2461 atatcaacat cgccgtcatt tctggtgata ctatcataga tttgggcaat acgcttttgc
     2521 caaaagatga taatattgtg attgtaagaa aagggatagg cagaacacgg atcttagtac
     2581 ctttgggcgt agccatccga ttagagcatg cgacattagt aggaaatgtc ttgtttgaag
     2641 aagagcaatt ttctttgaaa aatgaacaaa tcaaaatcta cagcagtgat tatgatgaaa
     2701 accctcgtag attgaagatt attactaata ccttgattgg agatgtcgag gtgatacgaa
     2761 tatgatggga aaaatatcca gagcgatgct agctgtttat tcggggattg cggctttcct
     2821 tattattcta ttttcacttt tcacttattt ttatgccagc aatcaaagtc attggtgggg
     2881 agaattactg cgtgcacgtt tattatatgt tccgcttatc ttccatttgt tagccatttc
     2941 cttaggtgta ggattaattg tctttctatt attatcactt attcaaaaga caaaatacgg
     3001 gaaaatcgaa gaaaagctgc gtgcactttc ttctggcaat tatgaatcca aattgttgct
     3061 tcttccgatc ccaagtgcat cggacgattt atacatcaaa gatattgata aggaaatcac
     3121 taagataaaa gaaaaaatga ttgaagtatc tagtgaatta caaattgtaa cgagccgtcc
     3181 ccaatatgta gatggacaaa ccaaagaaga aattttagaa ttagaaagac atcgattagc
     3241 ccgtgagctg catgattcag tttcgcaaca attgtttgca gcaatgatga tgatgtcggc
     3301 attgacggag caagcagaaa aaagcgagac accagaaatg tttcgcaagc agttgaaaat
     3361 ggtagcagag atcatcaacg cttcccagtc tgagatgcgt gcgctgcttc ttcatctacg
     3421 tccagtcaat ttagaagaga aaagtttaaa gcaaggtatc gagcagttat tgaaggaatt
     3481 gcaaaataaa atccagattt cgctgaaatg ggatgtagaa gatgtaaaac tatctagttc
     3541 cattgaggat cacttgttcc gaatcgttca agaattgtta tcaaatacat taagacatgc
     3601 taaagccaat gaattagagg tatatttgca caaaatagac aataatcttt tgttacgtat
     3661 tattgacgac ggaacagggt ttaatatgaa tgaaacgaaa acgggaagtt atggattgaa
     3721 caatatcaaa gaacgagtag ctggtattgg tggaacagta aaaatcatta gttttaaagg
     3781 tcaaggtaca agtgtagaaa tcaaggtccc tttgatgaag gaggcataga taaggatgat
     3841 aaaagtttta ttagtagatg accatgaaat ggtgcgctta ggcgtctctt tttatttatc
     3901 tattcaagaa gacattgaag taattggaga agctgaaaat ggacgacaag gctatgagaa
     3961 ggcgatgaca cttcgaccgg acgtcatttt aatggactta gtcatggaag aaatggatgg
     4021 tatcgaatca acaaaagcaa tcttaaaaga ttggccagaa gctaaaatca ttatcgtaac
     4081 cagttttatt gatgacgaaa aagtttatcc tgccattgaa gcaggtgcag cagggtattt
     4141 gttgaagacc tctaccgcac atgaaattgc tgatgctata agggcaacgc aacgtggcga
     4201 gcgagtatta gaaccagaag tgacaacaaa aatgatggaa aaaatgagtc ggcgaaatga
     4261 gcctgtatta catgaagaac taacgaatcg ggaaaatgaa attttaatgc tgatttctga
     4321 aggaaaaagt aatcaggaaa tagcggacga attgtttatt acactgaaaa cagtaaaaac
     4381 acatgtttcc aatatattgg ctaagctaga agtggaagac cgtacacaag ccgctatcta
     4441 tgcattcaaa catggtttgg tgaaataaaa acgatgataa agaggataat caaaagctga
     4501 gaaaataact attatttttc ttggcttttt tacaataatg atgcttgctt ttgtttttgt
     4561 aaaggctttg ttatactgag aaaagaagat actaaaggag gaaaacggtg aaacaaaatt
     4621 ttgcaattgt aggtcttgga cgatttggcg gaagtatctg ccgaacactg atcgaagctg
     4681 gtcaagaagt actggctatt gatagtaatg aagatcgggt caatgaatat atgaatatag
     4741 ccactcatgc tgttgtggca aatgcacagg atgaaatgac acttcgatca ttaggcataa
     4801 gaaatttcga tcatgtaatt gtcgctattg gagaagatat tcaagccagt atcttagtta
     4861 ctctgatggt aaaggaaatg ggcgtaccaa atattttagc aaaagcccaa aatgagtatc
     4921 atgccagagt acttgaaaag atcggagctg atcgcgtcgt ccatcccgag cgagacatgg
     4981 gaataaggat tgcgcataat ttagtgtcaa agaatatttt agattaccta gagttgtcag
     5041 ataaatattc tttagcagaa atccgagtct ccaatcctaa atttttcaac aaaacattag
     5101 ctgaattaaa tttcaggcaa cgttttgact taacagtagt cgccatacga agagtagatc
     5161 aaacggttat cgcttctcct gcagcagatg aatatgttcg tgaaaatgat aatctactgg
     5221 ttattggaga gacaggcgat gtggatattc tagacgataa gatgaatcaa tagaaagaaa
     5281 agagaaagta ggttgttggg atgaaattaa cggtaacgcc aaaagcacaa aagtggtttg
     5341 actcagaaat tgatctgcca gaaacttacg gaatacgttt ttttggaaaa gtctacggca
     5401 aaacagaggt tcatgatggt ttttcgattg gaatgtcagt tgaacagcca gaacatcctg
     5461 taaagaaagt ggaaattgat gggacacttt tttttatcga ggaagcagat gaatggttct
     5521 ttaaaggata tgatttactt gttgattatg atcccaacct ggaggagcca acctatcact
     5581 ttaaaaaaca ataaaaaaag agagggtgac acaatttttg tcaccttctc ttttttcttt
     5641 tgaaaatatt gttttcagct attaatatcc tgcagacaat tttacttgat tggtttgaag
     5701 ctcatgggtg gcaaagtagc ttttcagatt ggttaaaaaa atagccatta atttcttttg
     5761 aaaatcaggt gtcaaacctg caatgtggga agtaatcagt acattgtcca ttttccaaag
     5821 aggatgatct tgtggaagag gctcttcctc aaaaacgtct aatgctgcaa aggaaagttg
     5881 ctggttattt aaagcctgca ccagatcttg tgtcttaaca gaagcaccac ggccaacatt
     5941 gatgaataca gattttggat caaagtgttc aaacatatgc tgatcaaaga gcccagttgt
     6001 ttcctccgtt agaggtaaga tattgactac gaaatctact tcaggaagaa cttcctttag
     6061 ttgatttaaa gaaaaagtct tttccattcc ttctgctgga tgaccggatg tattgattcc
     6121 atagcagttc atgtctaatg ctttgcattt ttttgcaatc atttttccaa tatggccagt
     6181 tccaatgatt agtatttttt tcccttggat agattggtaa tggaccgatg aacctaacca
     6241 tttcatgtca ctctgtgcct tttgtgcttg aaaaagacca cgagaatacc caaggatgat
     6301 tcctaagaca tgctccgtaa tcgattcact gtggatgcca ctaccatttg aaagtaaaat
     6361 atgtttcttt gaaaaagtct caaggggtag ctgatcaaca ccagctgaga tagattggac
     6421 ccatttcaag gtagaagagg ctaatagttt ttcttggtag tctgagttcc agcccaaact
     6481 gatctctaca ttttgtagat cctctggttg taattctgtt ttaaagatat aaccaggcgc
     6541 tatagtatgg atttcttcga taaattcatc acgaaatgga cgatctgcat aaatgattct
     6601 ttctgacata gttaatttcc cgcctttctt tactctaagt gtagcgcata caagaagaac
     6661 tttgaagcgc cttgcttgtt ttttgagaaa gaaatatttt tcaaataaaa aaggggctgt
     6721 gacaaaaaaa tgtcacaacc tctaaaaggg tctattgaag tagaaataac gagttgtctt
     6781 tatttcttaa ttgatcgata tttttgccca tcctttgaca gcttgagcaa aaataaagat
     6841 tcctgcaaaa gcaatgaagg cgctagtgtc aaaagaagga agaaagacca tcaagattgt
     6901 tgcaattata gtaggcaatg aaattgaata gatcaatgtt tttaaacaat caagaaaagt
     6961 agcttttgtt agtcgaaagc gtgcatagag aaaagcagca atgttggtaa atagcaaagt
     7021 gatcagcaga ttcagaaaac taggataaat agaaaatata aatgtcagga cttttatcca
     7081 aagtggtata gatgcttcac ttaaaaaaga acggagctgc ttacctgtta agttttttaa
     7141 agattcattg gtgtagggaa gttctaattg gttattgttc aacaaagcag aagtcgtgcc
     7201 tgtattagga agagctatga taagtttgtt ttttaacatc ccgacactaa caaaattccc
     7261 catcaaatca gtagagatgt cctgttctgt tcgttttccc tctggatcaa atgtgaagat
     7321 aatggagttt gtttggtaaa tgaatccttc tttttcttcg gtttgaagct gcccgttttg
     7381 gattgtaaaa tcaggaatgc gagtagcaat cttttgccca tccaattgaa tgttgtttaa
     7441 gacttgaaaa acttgaaaag agattggcaa agccataatt atactcaaaa ctaaaagata
     7501 gacaatggct ttccaaaatg ggacatttct cgcctccttt aggtctttga atcgtataaa
     7561 ggaactgata attaattgtt ttgttgtcat aagtactcct catctagtag tgtcatcact
     7621 tgcaattcta gcgaagtatc aagtgaaata caagtgcttt aaggaaacag ctgaatgatt
     7681 ggtgaaatat caaaaaaatg ttattcttgt ctaaagacta tccaatataa ataaaatgga
     7741 ggaaggaata gtgcctacac agccacagag atcatcaaaa aaactatttg gagtagtggt
     7801 tttgaatgcg gtgatcactt tatcagaatt tattggaggg attatgtcag ggagtttgtc
     7861 tcttgtttca gatgctttcc ataaccttag tgatacattg gcaattattt tttcttacgg
     7921 ggcacaaaaa atggcccgaa aagaagcaaa taaaaaacgt acgtatggct accagcgttt
     7981 agaaatattg agtgctttca tcaactcatt catattgatc atattatcct tgtttttaac
     8041 cgttgaagca ttcaagcggt tcacttcacc agaaaagata aatagtcatt tgatgctaac
     8101 agtagcagtt attggattgc tcgcgaattt gttctcagta ttattattac gacaagaagc
     8161 agatgaaagt ttgaatataa aatctagtta tttgcatttg ttaagtgata cactgtcttc
     8221 ggtaagtgtg attatcggag caattttgat ccgttttttc gatatctact ggatcgatcc
     8281 agttattaca ctgatcattt cgatatatat attgatagaa gcgataatgg tgatcaaaaa
     8341 agctgccgct attttgatac aaagtgcacc tgctatcgac tatgaaaaaa tggaacaaga
     8401 aattgaagca atcgatgggg taaaggatgt tcatcatgtc catatttggc agtatagcga
     8461 aaagataatt attttcgacg gacatattga ttttgaagat cagctactaa gcgagattga
     8521 aaagatatat cctcgtataa tttctttatt aaaattaaaa tatggtatta cacatgtgac
     8581 gatccaagca gagacaaagg caaaggatca aaaaaaatta atttttttaa ataaagatga
     8641 aagttattga aatatagagt atgataaata agaattttta ataaggtagg ttggctcatg
     8701 aagcagtatc gacagttaaa aggatattta gaagcaaaag gatattggga gatactagtc
     8761 tatctctttt ttggtggttt ggctacgatt gtaaattttg ttagttttgc tctcgcacgc
     8821 caatattttg atttaagtat ggccttatcc aattctattt cgtggttttg ttctgtattg
     8881 tttgcttttg taacgaacaa actttgggta ttccattcaa aatcaccgaa ttttacacat
     8941 gcacttattg aatgcggtaa gttcttcttc taccgtatcc tttcttacgg attggacatg
     9001 ggggcaatgg tattgttgat caatgttatg aacagcaatg agtatgtggc gaaaatcatc
     9061 actcaaatta ttgttatcct tgcgaactat attttcagca agttgtttat ttttaaagaa
     9121 actgaggttt ttgaagaaga agtaggtcaa agtgaaaaag acgactaggc ataacaattg
     9181 aagaatttcc ttctctttgt tactctttgt aatgtagaca ttattaagaa aacattgagg
     9241 aggaattttt ttatgactta cacattacca gatctacctt atgcatatga cgcattggaa
     9301 ccttatattg acgaagaaac gatgcatctg catcatgata agcatcacaa tacttatgtg
     9361 acgaatttaa attcagcaat tgagaaatac ccagaattag gcgaaaaaac aatagaagaa
     9421 ttattatctg atatggacgc tattccaaca gatatcaaga cagctgtacg taacaatggt
     9481 ggcggacatg ctaaccattc atttttctgg gaaatcatgg caccaaatgc tggtggcgaa
     9541 cctacaggag aaataaaaga agcgattaat gaagcttttg gtgatttttc ttcttttaaa
     9601 gaagaattca aaaaagcagc cgctggacga tttggttctg gatgggcttg gcttgtaatg
     9661 gaaaatggaa aattagctat tacctctact gcaaatcaag attctccatt gatggaagga
     9721 aagacaccaa ttctaggttt ggacgtttgg gaacatgctt attatttgaa atataaaaat
     9781 gtccgaccag attatattgc agcattctgg aatgttatta attgggatga agtaaacaaa
     9841 cgtttcgaag cagcaaaata accatttcct ccgtcatatc agtcttaaaa ataatttgga
     9901 aaataaaaga gcgaaagctt gctagcgttt tctaaattgg gtataatgtt cttgcaaggt
     9961 tacttgcaag aacttttttg gaggatatac atttatggaa cacggaacgg ttaaatggtt
    10021 caacaatgaa aaaggatttg ggtttattac agtagacggc ggagatgacg tatttgttca
    10081 tttctcagca attcaaggag acggcttcaa gagcctagaa gaaggtcaag aagtagaatt
    10141 cacgattgtt gaaggtgcac gcggacctca agctgctgaa gtgactaaat tataataatt
    10201 tgttgaataa agacacgact tcttagagag tcgtgtcttt ttgtctattt atgaagtttt
    10261 tagatttttt ggctaagtaa atgcatttgt tcacttgaca tgtcctaact aattatcaag
    10321 caaggaacat tttcttcgtt agtaatggat tttaaagaaa tttcagtttt tggatgttga
    10381 ataaaagacc atttgtgaaa ccactagtat acatactcat taccatgtca attctattac
    10441 tttaaaagaa aatcgtttta ctttgaagag gagcatatct atccattaaa agatgatggt
    10501 aatgcaatca ctagttcgag tcattttaag agccatgcag tgagctggaa ataaaacgaa
    10561 tagaagatgg gtttaacaaa aggaccagtc ataggaagaa attgtcgtga aatcgaatga
    10621 taatctgaac ggtaggcaaa gactgttaaa aagatgggaa aggtatgtgc gatcatttta
    10681 ctgcacgatt atttggaaaa taccttgttg ttcgcttttt ctggaaaaaa tacataaaaa
    10741 aagataaaac gattgcgaac tgcgtgtatt cggtctttcg atctttt
//
LOCUS       DAZKLA010000099        10680 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000099 DAZKLA010000000
VERSION     DAZKLA010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.100"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..237
                     /locus_tag="ACG85N_002159"
     CDS             70..237
                     /locus_tag="ACG85N_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFQ0224886.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(297..650)
                     /locus_tag="ACG85N_002160"
     CDS             complement(297..650)
                     /locus_tag="ACG85N_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0224887.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            1154..1573
                     /locus_tag="ACG85N_002161"
     CDS             1154..1573
                     /locus_tag="ACG85N_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0224888.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            1599..1805
                     /locus_tag="ACG85N_002162"
     CDS             1599..1805
                     /locus_tag="ACG85N_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0224889.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(2033..2206)
                     /locus_tag="ACG85N_002163"
     CDS             complement(2033..2206)
                     /locus_tag="ACG85N_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224890.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            2218..2847
                     /locus_tag="ACG85N_002164"
     CDS             2218..2847
                     /locus_tag="ACG85N_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0224891.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            complement(2929..4095)
                     /gene="dnaJ"
                     /locus_tag="ACG85N_002165"
     CDS             complement(2929..4095)
                     /gene="dnaJ"
                     /locus_tag="ACG85N_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFQ0224892.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(4246..6075)
                     /gene="dnaK"
                     /locus_tag="ACG85N_002166"
     CDS             complement(4246..6075)
                     /gene="dnaK"
                     /locus_tag="ACG85N_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFQ0224893.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(6126..6689)
                     /gene="grpE"
                     /locus_tag="ACG85N_002167"
     CDS             complement(6126..6689)
                     /gene="grpE"
                     /locus_tag="ACG85N_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFQ0224894.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(6783..7826)
                     /gene="hrcA"
                     /locus_tag="ACG85N_002168"
     CDS             complement(6783..7826)
                     /gene="hrcA"
                     /locus_tag="ACG85N_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFQ0224895.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(8060..9225)
                     /gene="hemW"
                     /locus_tag="ACG85N_002169"
                     /pseudo
     CDS             complement(8060..9225)
                     /gene="hemW"
                     /locus_tag="ACG85N_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
     gene            complement(9241..9468)
                     /locus_tag="ACG85N_002170"
     CDS             complement(9241..9468)
                     /locus_tag="ACG85N_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFQ0224896.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            9952..10559
                     /locus_tag="ACG85N_002171"
                     /pseudo
     CDS             9952..10559
                     /locus_tag="ACG85N_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
BASE COUNT         3064 a         2165 c         1864 g         3587 t
ORIGIN      
        1 atatttttta ataaagattt gaattaaagt gtaaaggagg agatagttat tataaactac
       61 aagtggatat tgtgtcctgt atgtggaaat aaaacacgat taaagataag ggaagatact
      121 gaattaaaaa aattccccct ctattgtccg aaatgcagac aagaaaattt aattgaaata
      181 aagcagttca aagtaactgt gattacagag ccagacgcaa agacgcagag ccgataaaat
      241 gagattaata caatctcatt ttatcggctc tttccgttat gtatggattc ttttaattag
      301 tcttcgatgt ttcttgcttc gttgataccg ctggctaaag attccattaa ggatagttct
      361 ttgtctgtaa agctatccat gtatttctct atctgtaatc gtcgggtgct ttttaccaag
      421 ttattagcag gtaagaaaaa ttcatcaacg gaaacatgaa gtaacgatac aaggtcataa
      481 agaacttgta tgctggggtg ttgcccttta ttttcaatat tagttaagta ccgtgggtca
      541 atttcaatca atgctcccac ttgttcacga gttaaacctc gtttcaatcg agcttcttta
      601 atggctaaac caaaggctct aaaatcatat ttatcttctt ttttacgcat agtagaccac
      661 ctctatacat tttattgttc ctactgaatt aaaaacaggt atagaaaaac gtgttatatg
      721 gtttataggt ttatatttaa taaaaagcac tactaaacgc caataaaaaa aaccgttata
      781 tggtagtgct atttacgctg ttaaaatatt gtatattact tccaaatggc ggtttgttgg
      841 aggtcaacgt cgccatgaag tacatcatat acaataaatt tccttacatt gggttcttgt
      901 caaaaaaagt cgtctatctg caatagataa gtacgtccac caatgtggtt ttataaatca
      961 tatagataga ataacagaag catgtaaaca gagaaataaa tctgtttata tgcttttttg
     1021 gctattcaga acttttttac aaagtttatt tatcagtaat gcaacaaatc cccctttcac
     1081 attgggacta agagtgaaag gagataaacg agcaaggctc acttcctttc ctagacagaa
     1141 agggggtgag attatgagga catcttcttt cgagcatatc gtcagattac agttcaattc
     1201 tttaatgctg attgtcatta agaacaaagt gaaaagtcga aacagacagc ttgcaagaca
     1261 ttctaaacgt gaagtattgt tttgtgaaat agccgaaacg aaacaagttg aacgtggaac
     1321 gattgatacc tgtttttatg attgtgtatc atttaaagtc ttgcacttta caatttatgt
     1381 atctgatgaa acacttggta cagttttaca tagactatca gaaaaacagc gtagtgcgat
     1441 tttattacgt tattttcaag gaatgaacga cagagaaatt tcagaactgt accatgtatc
     1501 acgttctgct atttgcagta gaagaactag aggactaagg aagttaaaaa tgcttttgag
     1561 cgaaaggaag taaagattat ggctatccct cttttgaatt gatttgcaaa gcgtcaagcg
     1621 gagatgaaat ggcagttaaa gagattttga agttttatga cggttatatt tctaaattat
     1681 gcttgcgtcc tttttaccac tctgaaagtg gtaaaatcat tatgcaggtt gatgaagaac
     1741 taaaaggaga aattcataca gacatgatga aagcaatatt gaaatttgaa atcggagtga
     1801 agtaatcaac tgtataaaaa aatcgggaga tacacagttt cgtgttatct cccttagtag
     1861 ttttacggtc atatcaaggc tcattcaatc gtggtaaatt cgtggtaaga tgagtgtttt
     1921 tctatacttc aaaatgcttg aatgtatagt gtttatgcgg ataattgaaa attagatata
     1981 aaatttaata aaaaccaaaa atgcacggtc gccatgaagg gaccgtgtat ttttatcttc
     2041 ttattttctc acctaatcgt atcagccgca tcgcttgttt tcctgcacaa acaagcaatc
     2101 gctctacctg ttccatcgca taagaaagta ccatcggttc attgatgata tcaagtacat
     2161 agtcgatacc accctcactt aattgagctt gatttttctt ctccatatta tgtagttgtg
     2221 ggacaacttt ctgacaatca gcaattgtgt caagaacgga tcaatccttt attggagttg
     2281 ctcgaaaggg tcttctctta ttacggacag gctttgctca ctgtacatag acaacaaatc
     2341 attatcctag tcaatcggat ttccagagcc tcattgctat cattgcttga taaaatccag
     2401 acaaagttta ataaaatgta tcagttacag ctaaactttg gaattgggtc gctttgttat
     2461 acagagcaag agacaccgca atctttcctt catgccaaac aagtttgcga atggatagct
     2521 ttccatcaat ctgtgaacga gatccgcttt tttgaagatt tagacctcgg gatcgtttta
     2581 ccagcaatcc cgtcagatca acgaacatta tatgtaaaac gtattttgaa aagcctgaca
     2641 gaagaagaag tacatttgtt caagaagact ttggcatgct tcagtaaaaa taacggaagc
     2701 atcaaaaatt gctcagaaga attatttatt cataaaaaca ccttgcaata tcgattgaac
     2761 aagttccatt ctctcactgg ttatacccct agaaactatg acgattatca tattctaaag
     2821 ctggcttttt tgttggtaca aacatgacaa ctacaaaaaa accaggcgaa catatgattg
     2881 ttcgtctggt ttttaattat taatgactta gcgtttttac tagaaaaact acttccgctt
     2941 tttcccgcca aaagcatctt taaacttatc gaagaaaccg tcttcttgct gttcttcaac
     3001 tttgatgccg ccagcttcag cgaatgcacg taatgcttct ctttgttgtt catttagatt
     3061 tttcggtgtg atcagtttta ctttgacatg ttggtcgcct gttgcaccac cacgtaactt
     3121 cggagcgcct tttccacgta aacggaagtt cgtacctgtc tgtgttccag cagggatctt
     3181 caatttcaca tctccatgga cagttggtac ttggacttca tcccccaaag ctgcttgtac
     3241 aaaacttaaa ggcaattcat agtaaatttc agaaccttca cgatcaaaga tatcactttc
     3301 ttctacacgg aagaccacat acaaatcacc aaatggtccg ccgttttctc cagcttctcc
     3361 ttgaccagct aaacgcattt gttgtccttc ttctacacca gctggtacat tcactttaac
     3421 tgaatgtgct ttcttctcat gtcctgagcc atgacaggtt ggacaaggtt ctttgatttc
     3481 tttccctgtc ccatgacaaa catcacaagt ttgacggctc atcactcgtc caagtggtgt
     3541 ttggcgttct acattaatcg tacctgcacc atgacattta tgacaagtag ttggttgtgt
     3601 gcctggtttt gcaccgttcc caccacaagt atgacagatt tcttcccgat tatacttgat
     3661 ttctttttct acaccaaaaa tcgcttcttc aaaagacaag tcgaccgtat attgcaaatc
     3721 agcaccttga cgaggcgcat ttggatcgac tgaccgaccg cctcctccaa agaaagagtc
     3781 gaagatatct tcaaaaccac cgaagccgcc accagagaag ccgccgaatc caccaaatcc
     3841 gccaccagct ccaccagcac cgtaatttgg atctgtgcct gcatgcccgt attggtcata
     3901 tgcagcgcgt ttttgcgggt cacttaaaat ctcatatgct tctgatactt ctttgaattt
     3961 ttcttccgca tcaggttctt tattgatgtc aggatggtat tttttcgaaa gcttacgata
     4021 agctttcttt atttcatctt ctgatgctcc ttttgagagc cctaagactt cataataatc
     4081 acgttttgtt gccataggct ttcctcctat tggttctcat cattttcacg tcgcttgtta
     4141 tcatatcata ttttgtttga aacaaaaacc gatacttcaa actatttttc aaaagaaaaa
     4201 gccaaagtag caactttggc cttctctcga aattcagtga actgattatt tatcgtcgcc
     4261 atcgacttct tcaaaatcag catcaacgac atcatctgct ccgccttgag ctgcttctgg
     4321 gttttcttga gcttgttgtt gtgccgcttg ttcgtatagt ttaacagtca agttttgaac
     4381 gatttcgttc aatgcatcgc gtttagtttt catttcttcg atgttgtttg cttcaacagc
     4441 ggctttcaat tcatcgcgag ctgtttctgc ttttttcact tcttcttcgt ccactttgcc
     4501 ttctaattct ttcaatgttt tgtccacaga gaatagcaat gcatctacat cattacgtag
     4561 gtcaacttcc tctttacgag ctttatctgc ttctgcattt gcttctgcat cttttaccat
     4621 acgttcgatt tcttcgtctg ttagacctga agaagatttg atcgtgattt tttgttcttt
     4681 ttgtgtacct agatctttcg cagatacatt tacaatcccg tttttatcaa tatcgaatgt
     4741 tacttcgatt tgtggaatac cacgaggagc tgctggaata tctgtcaatt ggaatcttcc
     4801 tagagttttg ttgtcagcag ccattgggcg ttcaccttgt aatacatgga tgtctactgc
     4861 tggttgatta tctgccgcag ttgagaatac ttgtgattta cttgttggaa tcgttgtatt
     4921 gcggtcaatc aatttagtga acactccacc cattgtttcg atacctaatg acaatggtgt
     4981 aacgtcaagt aaaacaacat ctttcacatc accagtgatc acgccacctt ggatagcagc
     5041 acccatagca actacttcat cagggttaac tgatttgttt ggttcttttc ctgtttcttt
     5101 acgtactgct tctacaactg cagggatacg agtagatcca ccgactaaaa taacttcgtc
     5161 gatttctgat tgtgataaac ctgcatcttt tagcgcttga cgtactggta cttttgtacg
     5221 ttctactaag tcagctgtta attcatcaaa ttttgcacgt gtcaatgtca tttccaaatg
     5281 caatggtcca gattctcctg ctgtgataaa tggcaagctg atttgtgtgc ttgatacgcc
     5341 agataaatct tttttcgctt tttcagcagc gtctttcaag cgttgtaaag ccattttgtc
     5401 ttgagacaag tcaatgctgt tttctttttt gaattctgct accatgtagt cgatgatttt
     5461 gttatcaaag tcgtccccac ctagatggtt gtcgccagct gttgacaata catcaaagac
     5521 accgtctcct agttcaagga tagatacgtc aaaagtaccg ccaccaaggt cgaatactaa
     5581 gattttttcg tcgcgatctg ttttatctaa accataggca agagcagctg cagttggttc
     5641 attgacgata cgttctactt ctagaccagc gatcttacca gcatcttttg ttgcttgacg
     5701 ttgtgcatca ttaaagtaag ctggcacagt gataactgct ttttcgactt tttcacctaa
     5761 atagtcttct gcgaatcctt tgatatattg aaggatcatt gctgagattt cttgtggtgt
     5821 atatgattta ccttctacat ctactttgta acctgcttca ccgatatggc gtttgattga
     5881 tgaaatcgta tttgggtttg tcacagcttg acgttttgca acttccccta cttggatttc
     5941 tccatttttg aatgatacaa cagaaggtgt tgtacggttt ccttctgggt tagcgatgat
     6001 ttttgcttcg tttccttcca atactgctac tgcagagtta gtcgtaccta agtcaatacc
     6061 gataatttta ctcataatta acgtctcctt ctttaattga aagtgtaagt tttatatccg
     6121 cacaattatt gtgcaacaat gaccatggtt ggtcttaaca cgcgatcatg taatttgtag
     6181 ccttcttgta gtacttctac aatcgtatcg gctggtgtat cttcagtcgc tggaacagtt
     6241 tgtactgctt gatgaagatt aggatcaaag gcttctcctt tagcagggat tttttcaatt
     6301 ccttcttctt ccagtgcgtt acgcaaactt tccaagacca tttccacgcc ttttttcaaa
     6361 cctgcccctt gatcatctga cacttctgtg gctaatgcac gttccaggtt gtcaattgat
     6421 ggaagtaatt ttttggctaa atcttgtgaa cggtattttt gtaattgctc acgctcattt
     6481 tttcctcgat ttgccatatt tgcgatttca gcacgagccc gcaagtattt atcttccatt
     6541 tcttctaact cagcttttaa tttcgctgtt tcgaattctt ccgcttcagc ttctgaaata
     6601 cccgaatctt ctgcagcttc gacatcgatt tccggttctg gctgagcatc tgtcatttct
     6661 ttatctagtt cttcttgatt ttctttcacg atacagtctt cctttcatta ttcagtcagc
     6721 tgtttgattg ctcttactaa gaaagagaga acgatcagct gcaaatagct tcgttattca
     6781 cattacgagt ttgacaagtc aagcgaacgg tagtaatcag caagtttagc tgctagttct
     6841 cttcgataaa catcgactag accgaacatc cgagaataag gcatactagt aggccctaac
     6901 aaggctattg tacctcttcc atgaccatct atctcgtaac ttgcttgaat catactcata
     6961 ttctgcaata aatcattgcc taattcagca ccaatcttga tatgaatatt gctatccatc
     7021 ggagtgatca tttgtgtaag tccgtctgag tctttcataa aagaaaacat cgatttgaac
     7081 tgatcaagat cttgacgggg ctcataatcc aagagattca tttgtccact gacataaacc
     7141 ttatcttcaa aaacatgacc aagaacggtg tcaaataact gagacatccc ttctgtcgtt
     7201 tggaaatatt tatgaagaat catcggaatc tccgtacgaa gtttatggta caccgtcatc
     7261 agaggatctc cgactagacg gtcgtttaca atctgtacca ttttttctag ttcctgactt
     7321 cccatatatt cgggaagagt gaacacttgg ctttctacat tccccatatc tgttacgacg
     7381 atagcaataa tctgtcgatt attcaagggt acgatacgaa agccagtcag tcggcggtct
     7441 tttatttcag gtcccaatga aaaagtcgta tagcttgtca gctcagataa tatctcagca
     7501 gattgcttaa taatctcatt gatttcatga aattctctgc ctagagactg acgaatcagc
     7561 agcaaatcat cttctgctac gcgagcaggt tttaacaaat gatccacgta ataacgatag
     7621 cctaaagcag atggaatccg cccagaagaa gagtgagtct tcaacagcaa tccttcatct
     7681 tctaatgctt tcatatcatt gcgaatcgtt gctgagctgg ccttgactcc ctcagacatc
     7741 aatgttttgg aaccaacggg cacgccacta ctcgtatagt gttggatgat cagacgtaaa
     7801 atgtcgtttt gtcgctttgt taacatcttc ctcacctctc attagcactc atatattcta
     7861 agtgctaatc cacttataaa tatagcaaga agcccttctc ctgtcaagag aaagactcca
     7921 ttttttagca ctctaaacac aagagtgcta attctaaata attgatgtaa atccagttat
     7981 atcaacgttt caatagtcct ttattgtatt tttgaacttt ttttatttaa attcagataa
     8041 aaaacaaata ttctttaaac tatttttcaa acaaaaagcg ctcaaacaca ttgttcccca
     8101 aaaaggtccc tcttggtgtc aaataaacac ggtcttcttc attaacaagt aacccttctt
     8161 gctctaattc ccttagcaca tcaccgtaca ccgattcaat tgttttttga tagcgatcat
     8221 agaaatgttt tttagtaatc cctgtttttt tcttagtcct aagaacattt cttcttctat
     8281 ttgatttttt cgtgtcaatt tttcttcttc caataccggg cgttcatttg ctcgtaatgg
     8341 gcgtaaatag tgttggattg gtcctttgtt gcggtaacga atatctccaa gataaccgct
     8401 agcaccagct ccaaacccaa aatagtggtc attgttccag tacatgaggt tatgttgaga
     8461 ttcatgtccg gagagcccaa agttacttat ttcatattgg tgacgtccat gcttttccat
     8521 tgcttcaatc gtctcttcaa acattctggt ttccgtttcc tgatcaggca gttcgagtct
     8581 tccttgtcgt acccagttca tgaacattgt tttgttttcg aggatcaatg agtacaaaga
     8641 atagtgaggc agatccaaag ccaaagcttt ttccaaagta tccagatatc cttctagtgt
     8701 ttgtcccggt aatgcataaa tcaaatcgat gcttacattg gtaaaatttt ctgcttctaa
     8761 aaattgcata gtttgataca catcttctgc ggtgtgttta cgtccaattt ttttcaataa
     8821 gcgattatca aatgtctgca ctcccatgga taaacgattg acgccatagt ttttcattac
     8881 ttgaagtttt tcacgggtca aatcacctgg attcgcttct acagtaaact cattaccatt
     8941 ttgaaaaggc aggatctctc tagcacctga taacagtcga tcaagttgtt tagcagataa
     9001 agaagttggt gtcccgcccc ctacgtaaag tgtttctaaa ttcgctgtcg gatgactttc
     9061 tagcattatt ttcatttcat tcagcagcat ttccacatac tcatctactg gctgcccttc
     9121 taaaaaaact ttattaaaat cacagtaata gcagatatgc tcgcaaaatg ggatatgaat
     9181 gtacgcagaa accttgtttt ctgcgggatt tattgtttct atcattgaaa ttctttctcc
     9241 ttacgtcgaa aagtcgtgcc tgttttcgac cttcttaaaa tcgcgttcaa gcaccaactc
     9301 attgaaataa gccgcatatt tagaatcaag gaaacctagc tctaaaaatg cttgcttatt
     9361 gtcatcggag ctacacggtg cttttacagc ctctcgataa acggtaatcg caaagctgac
     9421 cttcggcatt aagtcaaaat ctgcaaaaat atgaaaagct attttcacag attcctcctt
     9481 aagtgctcag accacaacgc tttgcttaca acctctcata tacgcatact aaacgtattt
     9541 ggcgaacaaa tccactaaaa aatccaagaa tcgacttctt ggatgatttt ttctttcatt
     9601 aaactggtta cctagttata tatctctagg gttgttttca tgtctgagtg cccgatatga
     9661 tttatttttc gtttctataa ttactttttg ccttccagtt tcttttaata ttgtactcaa
     9721 gcaagtggga attggttctt ttgtcatcca tgtatgcaaa atgttataat gctggtaaac
     9781 aaaaatgatt acctgtcatt tttatttagt tcgtatttaa ttaagttgac tatctgtata
     9841 ctctgtctaa aacttaagaa ttgttgtttt cgctgtcgct acccaatttt aatgaatgtc
     9901 tgtaggttaa aactgttgtc cttgttatag atagtaaagg agagcttgaa aatgaaaact
     9961 gcgattacgt ttctattaga ggaatatgcc gattgggaag gtgcttatat tactagcaca
    10021 ctaaaccaaa atgagaaatg gcaaaacaaa actgcttcta tccaatcaga ggtcacttcc
    10081 attggaggat taaaaacaaa aatcgattac ttaatcgaac aaatacctga aaaagttgac
    10141 ctttttatta tgatcggtgg caattcatgg gacataaaaa atcatcattt atatcaaata
    10201 atcgataaat tgttaaaaac agacgtacca gtagctgcta tttgtggggc agttgattat
    10261 ttagctcaga gcggattatt gcaaggttac aaacacaccg gaaatgcgca atttccttgg
    10321 ttagaatcgg aaaattatca gaataaagaa gattttatag aacaacaagc tgtgaaagat
    10381 cgtaatttga ttactgcgaa tggaacagct cctatagaat tcacagactt gattcttcaa
    10441 gcagtccatt ttgaaacaga agaaataaga gaaaaaacga ttcgtctata caaacttgga
    10501 ttctatgatt actataaaaa gtacggagat cctttctcct aatcattctc ttcaattaaa
    10561 tactccttat caatagtgtg acaagtaatt ttactgatga ttatttgtca cactttttct
    10621 gacatttatc aaagaagata cactctattt atattcatag acaaggtttt acggattcaa
//
LOCUS       DAZKLA010000100        10376 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000100 DAZKLA010000000
VERSION     DAZKLA010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.101"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..335)
                     /gene="acpP"
                     /locus_tag="ACG85N_002172"
     CDS             complement(93..335)
                     /gene="acpP"
                     /locus_tag="ACG85N_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0224897.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(457..1458)
                     /gene="plsX"
                     /locus_tag="ACG85N_002173"
     CDS             complement(457..1458)
                     /gene="plsX"
                     /locus_tag="ACG85N_002173"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFQ0224898.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(1507..3543)
                     /gene="recG"
                     /locus_tag="ACG85N_002174"
     CDS             complement(1507..3543)
                     /gene="recG"
                     /locus_tag="ACG85N_002174"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFQ0224899.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(3660..5342)
                     /locus_tag="ACG85N_002175"
     CDS             complement(3660..5342)
                     /locus_tag="ACG85N_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFQ0224900.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(5394..5756)
                     /locus_tag="ACG85N_002176"
     CDS             complement(5394..5756)
                     /locus_tag="ACG85N_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0224901.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            6026..6214
                     /gene="rpmB"
                     /locus_tag="ACG85N_002177"
     CDS             6026..6214
                     /gene="rpmB"
                     /locus_tag="ACG85N_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFQ0224902.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            6452..9121
                     /locus_tag="ACG85N_002178"
     CDS             6452..9121
                     /locus_tag="ACG85N_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFQ0224903.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(9576..10322)
                     /gene="truA"
                     /locus_tag="ACG85N_002179"
     CDS             complement(9576..10322)
                     /gene="truA"
                     /locus_tag="ACG85N_002179"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0224904.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         2832 a         2235 c         1865 g         3444 t
ORIGIN      
        1 cagaaaaatg acttttacgt gtcaaaaaga ttaaaaaaag aaggataaaa agctgcatgc
       61 agctttttat ccttctttgc ttttatttga aattatttaa gatggctgct gatataatct
      121 actgcgccac cgactgtttc aatttgttca gcgtcttcat ctgaaatttc cgtaccgaat
      181 tcatcttcaa gctctaaaac gaattccata atactaattg aatcagcttt taaatcatct
      241 ttgatgttca attcatccgt cactttatcg gtatctatct caaaatgatt tgaaataatt
      301 ttcgctactt tgttaaatac ttcttcgcga gtcaactttt tcacctccaa gaatcataca
      361 cttgtcaatt acctacacca tggctaattt tactggcatt tttcaggaat gtccaccttt
      421 ttttcaatgg aacggatcgc ttcagatatc caaatactat tcttcgcttt tttcaaattg
      481 ttcgactaat tgtccaacta catctgtctc aagcattgtg tggatttggc ggatcgtata
      541 gcgaacagca tcggggcctg ttgctccatg agttttaatg actggtgctt tcaagccaaa
      601 caaaacggca ccgccatgtt tggaataatc catttctgct ttcaaagaac gtaagccgtc
      661 ttttaaaagc atagcgccca tttttccttt gatcccttca tttagaatag cagattttaa
      721 tagattcatc atattcattg ctgttccttc aatagatttc aaaacagcgt tgcctgtaaa
      781 tccatctgtg accactacat cagccacacc attgagtagc tcacgagctt ctacgtttcc
      841 aataaaattc aagcttgttt cattctgaag gagttcatag gcttttttcg tcacttcact
      901 accttttgtt gcttctgttc cgttgtttaa gagtgcgaca cgaggacgtc cgatacctcg
      961 gactttacgt gcataaaaag aacctaaaat accgtattgc aacaaatgtt ctggtttgtt
     1021 ttctgcgtta gcgccaagat ccagcatatc gaaaccggca ccttctttcc ctactactgg
     1081 caaggtagac atcaagccag ggcgttcgat atttttgata cgtcctacga tgaacaaccc
     1141 tgctgctagt agcgcgcctg tattccctgc tgaaaataca gcatctgctt caccgttttt
     1201 cactgcttgt gcagcaagga ccattgatgc agtctttttt cgtcgaattg cttttactgg
     1261 ctcatcatca ctattgattt tttcgtctgt atggataatc gtgatgtttt tttcatttgt
     1321 taagtatttc ttgatttctg cttcttttcc gtaaagtaag aattctactt ctggaaaatc
     1381 ttgctgcgct aaagccacgc cttctacaat tgcttgaggt gcgtgatctc cgcccattgc
     1441 atctacggca attttcattg tttccactcc tttttccgtc atcagtataa agtatagcac
     1501 agtctgctaa tcgaaataac gattatcctg attttctatt tggaggcttt gcgctaaagg
     1561 ctgatactca ggaagcatct gccaatcctt tatttgccaa agggctgcag cctcttctct
     1621 ggctacctcc aaaacatttc catccgtcac caagtcagca atcgcaaatt gaggcagacc
     1681 agattgacgg aagccaaata cttcaccagg accacgtaat tccagatctt tttcgcttag
     1741 tacaaaacca ttggtggtct ctgtcatgat tttcatccgt tccacaccta gttcattttt
     1801 aggattagca actaagatac agtaggaagc atcacttccg cgtccaacac gtccacgaag
     1861 ttggtgcagc tgtgccaatc caaatcgatc cgcatccatg atcagcatga cggtcgcgtt
     1921 cggcacgttt acccctactt caattacagt agtcgaaact aatatctgca tctggttgtc
     1981 tttgaaggct tccatgatta cttctttttc ctgattcttc attttaccat gtaacaagcc
     2041 aacttcatat tttggtgcaa atagttcacg aagtttttca tagatttcca ctgcattttt
     2101 tacatctaat gcttctgatt cttcaatcag cggacagatg acatacattt gatgtcctct
     2161 tgccagttct tttttcgtcc aatccagtac actgtccaat tgaggtgttc ttacccagcg
     2221 tgtttcgatc gggatacgtc cagctggaag ttcatcaatg atagatacat ccatctcacc
     2281 atatgctgta atagctaatg ttctaggaat cggagtcgct gtcataaata agacatctgg
     2341 tcgccagcct ttttcacgaa gtactttccg ctggttcacg ccaaaacggt gctgctcatc
     2401 cgtaataact agacctaaat tctgaaattc gactccatct tggatcaatg catgtgtgcc
     2461 gatcacgacg ttaatttccc cttgctctag tttttcaagg agctcacgtc gttcttttgc
     2521 tttcgttgac cccgtaatca atgcgatggt tacttcattg ggatcaaaca gctgttgcaa
     2581 gctttccata tgttgttgtg ccaagatttc tgtaggcacc atcaacgcac cttggaaacc
     2641 tgctgtaacg gtagcgtata acgcaatcgc ggcaaccact gtttttccgc tacctacatc
     2701 tccctgcaaa agtcgctgca tatgctgcgg actgcgaaga tcccgacaga tttcattcgt
     2761 tacacgtttt tgtgcatttg tcagttcaaa aggtagcttt tgagtaaatt gtttcaattt
     2821 ttggacatca taaaggatct ccaaaccatt cgtttctgct ttttctgctt ttttcaatcc
     2881 ttgaagacgc atttgaaata aaaagaactc ttcaaaaacg acacgtcgtt tggcttgatg
     2941 gttttcttcg tggtcttttg gaaaatgcat acttcttaca gcagaagctc ggtcaagcag
     3001 tcgatatttc tccacaaggt tgtttggcag attttcttca atcaatgtgc cgtattcttg
     3061 aaaccctttt ttgattagat caactaatgt cgtttgacga attgatttgt tgacgtgata
     3121 tatcggcgaa aagtcatcca ttgtttgaga acctaaaatc ttcatcccgt tcaaggcttt
     3181 acgctttgca tcccatttac cgtatacggc gatttcctcc gataaaatga ctttttcttt
     3241 tagataaggt tgattaaaaa aagaaacgtt gaacacatca tgttcttgca tcatacgaaa
     3301 ttgcaatcgg ctttttttat aaccaaatcg attcatcaca ggaggcgaga ctacgattcc
     3361 tttcaaagta accttttctt gatcctggat ctcgtttaga tttctttcct gaatatcttc
     3421 gtagcgaaaa ggataataag tcagtaagtc ttcaatcgtt tggatgccca agctggcaag
     3481 actagctgct cttttttctc cgactcctgc cagcatagtg attggatcat ctaaagaacg
     3541 cataaatttt tcctccctcc tttaacaaaa aaaggacttg gtcagcgatc atacctgatc
     3601 actaaccgaa ggccttttat gaatgaatac tgtgttagaa agatattctg acacagaaat
     3661 tattcagcag aaaatagata aggatagaca ggctgatctc cttggtggat ctcgatttcc
     3721 aaatcaggat gtttttctgt tagtttctca gcaaattcat ttgcttgttc aactgttcct
     3781 tcatcacctg ccaagatcgt tacgatctct gtgtcctcat tgatcatttg ttctagtgta
     3841 cttaacgctg ttgctgtgat atctgggtta gagatcagga tttttccatc gatcatgccc
     3901 aaaaagtctc cttctttgat ctctacaccg tcaattgagg tatctctgat cgcgtgagtg
     3961 acttgaccgc tcacaacgct agacaacatt tctgtcatcg tttctttgtt ttcttctaaa
     4021 gaagcttgat cattgaatga aagcatagct gtcaatcctt gggaaatcgt tcttgaagga
     4081 acaactgcta ctgggatatc tgctacttct gctgcttgat cagctgccat gaagatgttc
     4141 ttattgtttg gcaagacaat cactttatct gcgtttactt cttgaatcgc tttaacaatg
     4201 tcttccgtac ttggattcat tgtttgtcca ccactgataa cataattagc gcctaaactt
     4261 tcaaacagct cttggacgcc tttaccagca gcgatcgcta cgataccata cgggatacgt
     4321 ggtatttctt ttttcgcaaa ctcgttcact tcttcatcgt gttccaaaat cgtctcatgc
     4381 tgcaagcgca tgttgtcgac tttgattttg actaatgaac cgaatttttg tccataattc
     4441 aagacttctc ctgggttttc tgtatgcacg tggactttga tgatctcgtc atcattgacc
     4501 acaagtaaag aatctcctat accatctaaa tagttacgga attcttcgta atcgaatgta
     4561 ctatcgactg taggtccttc tccgattttt accatgattt ctgtacagta accaaaatgg
     4621 atatcttctg tcgccatttg accttggaca ctgcgatgat gttctgcatt gaccatatca
     4681 tccatttctg ctggagaagg ttcatatgtt tcatccgctt ggaattctcc attcaatgca
     4741 cttaaaaagc cctcataaac aaacagcagt ccttgccctc cactgtctac gacaccgact
     4801 tcttttagga ctggaagcaa gtctggagtt ttagctaaag cacgctttgc tccttttaca
     4861 acagctgtca ttacttcaac gacatcatca gtactttttg ctttcttttc tccagctttc
     4921 gctgcttctc tagctactgt caaaatcgtt ccttctactg gtttcattac tgctttatac
     4981 gcagtttcaa ctccatgagt gaaggctgca gcaagatcag aagcatttaa tgagacaaca
     5041 tctggaattt gcttagaaaa tccacgaaac aattgagaca aaataacgcc ggaatttccg
     5101 cgcgcaccca tcagcaatcc ttttgaaagc attgctgcta attctcctac tttttctgag
     5161 gttgaatcta ctactgcctt agctccacta gtcatagata gattcatatt tgttcccgta
     5221 tcgccatctg gcacagggaa tacatttaat gaattgacgt attctgcatt tacttgcaga
     5281 cgattcgcac ctgcctggac catttcctgg aactggcttg cacttatttc tgttacactc
     5341 acctagataa tcctccttca gatcgactgc tgagttacca gccagtctcc tgcctagtct
     5401 ggcagtacac gaacaccttg tacaaagaca ttaactgaat tggcagttac gcctaataat
     5461 gcctctagat tgtatttcac tttttcttgg acgttgcggg aaacttcaga gattttcgta
     5521 ccatagctga taatcgtata aacatctaca gcaataccat tatcttcttg gcggacaact
     5581 actcctcttg agtaattttc tttacgcaaa atctcagtaa tattatcttt aatttgattt
     5641 ttgctggcca ttcctacaac accgaacaca tctgtcgcgg cgccaccaac aactgttgca
     5701 attacatcat tggtaatttc aatggttcca gctgacgttt gaatttttac agccatgaat
     5761 gagcctcctt aacgggtaaa aaactcccat ttttcttcta tcatatttta tcatagcgca
     5821 acttgaaata aaaggcagtt acccaaagta ctcaaaaacc ttgcaaaatt tcaggtataa
     5881 ccttgtttaa tttacaatta ttcttctcaa ttgtcaattg tatctatgaa aatgtacttg
     5941 caaattccca ttctatatga tatgttagtc tggtatgagc tgaattattc tattgctcca
     6001 aatcaaggca aaggaggaaa ttaccatggc aaaagtttgt tactttacag gtcgcaagac
     6061 aaaaagtgga aacaaccgtt cacacgctat gaactcaaca aaacgtactg ttaaacctaa
     6121 cttacaaaaa gttcgcgtta tggtcgacgg aaaacctaaa aaagtttggg tgtcaactcg
     6181 tgctttgaaa tcaggaaaag ttgaacgcgt ttaattgaat gatacttgga ccgttcagat
     6241 agtgaacggt tttttattta ataaaaattt ttaagggtgg gacttttgat tcctaccctt
     6301 tttttgcgtt tatttttgtt atacgaacaa attatgaagt tatttattct aaattttatt
     6361 tttttgtgaa aactagactc tttttaagca gagaaaaaca aggatatgat aaactgaata
     6421 caacttcata gaaatttggt ggaaaataaa tatgaaaaaa aatttaaagt cattctttag
     6481 agaaaacgga cgctacgcag cagctagttt cctgattccg tttttgattt tagcacttgt
     6541 ttatctaagt atcggaatct atcctggaag cagcagaagt attttggcaa gtgatgcctt
     6601 ttcacagttt tctaattttc atgccagctt ccgaaatgtt cttcttggaa agcaaagtat
     6661 tttttataca tggaatgctt ctctaggatt gaattattta tctttaattt cttactatct
     6721 aggcgggatc ttcacgccac tggtgttctt ttttccaaac cagatgatgc cagatgcact
     6781 gtatgttctg actttgctta aggtcggctg tgccggattg gctttttggt ttcttgcacg
     6841 tacgttcaag attcctcgct gggggcatgt tgcacttagc gtcagttacg cattgatctc
     6901 atttatcact gcacattcgg agatcatcat gtggctggat gcattttttt atcttccatt
     6961 agtgattttg gggattcatc gtgttatgga tcttaaacgg ccgaaacttt tatttatcag
     7021 ttatttactc ttgtttttat ccagttttta tatgggtttt atgattggcg tattttcttt
     7081 cttatatttt atcgcccgtc tattgacaaa ctggtcagaa tataaaaaaa cgattgttcc
     7141 ttatggtatc acttctttac tggcaggcgg cgcttcaatg atcattgtat tgccagcagt
     7201 gctggatctt cggtcaaatg gcgaggcact gagtcagatc actacgttga aaacagaagc
     7261 gacttcttat ctggatctaa tcatgaaaaa tatgattggt gtttatgaca cgacaaaata
     7321 cggatcgatt ccgttcgttt acgcaggtct gctaccatta gtcctctgtc tgttttattt
     7381 tgccagtcgg aaagtaccgt tgaaaaataa atttttgtac ggcagtcttt tcgccatact
     7441 tatcgcaagt ttttatcttg ttccattgaa tttgttctgg catggtatgc atgcaccgaa
     7501 tatgttttta ttccgttatg cctatctgtt ctctttctta gtgctcctgc ttgcaggaaa
     7561 aggatgggaa aagatagaac aagacgatcg tgggctgttg ttaggaatca ttttggtcgt
     7621 agctgctctc tttgcggtgg catggggcat caaacctgaa ggaagctaca cctatgtgac
     7681 gatgacttcc tttgtattga cagtggtttt cttagggatt tatgcttttg ctgtgggatt
     7741 ttatcagcat aaaaaacttt cattcaatca tctgagtata ttgttattat tgatcatgtc
     7801 tgctgaagca ctggtcaata cgaacagtat gatccatggc attcttgatg attggaatta
     7861 tgcttcccgc agtttatata ctgatcctta tcctgacttg aaagctcttg taaatgaaac
     7921 aaaaaaagat tccgactcgt tttaccgctt ggaaaatctg gatccagtat ctgcaaacga
     7981 ttcgatcaac tatggttaca gtggtatcag tttgttttct tctataagaa atcgtaattc
     8041 ttcctcttac ctggataaat taggtttccg ttcaagaggg acaaatttga atatccgtta
     8101 taacaataat actttgctga tggatgggtt tacaggaatc aaatataaca tttctaaaaa
     8161 cgaaagcacg ttcagtaaat atggcttccg ccaagctggt caaagcggcg actatacact
     8221 gtatcagaat gtcaatgcat tgccactagc atttactgca ccgttgtccg ttaatgatgt
     8281 tgagcagcca gtgacagata atttgaccag tcagacaaac ttgatcaata cattgtctgg
     8341 tctaaatgaa cagtatttta cattctatac acctactttg aagaatcagt tgaatacgac
     8401 gatcacaaag acaacgactg gcgtcactta ctcagaaaca gcagataacc agccaaaaga
     8461 gctcacttgg gaagtccatg tgcctgcaaa tacacaagct tacttaagtt tgttcccaac
     8521 caattttgcg caattagaaa gttctagtgc gacgatcact gtcaatggta catcgcgaaa
     8581 aacgcaaatc aacatttctg gtcaatacta tgatcttggc tattatcctc aagagacaac
     8641 agtgacattt actgcaagtt tttatgggac gaaagaaata agtttcatgg agcctaaagt
     8701 cgttggtcta gatgttgtag cctatcaagc cgcaatgaat cagatcaaag aaaaaggtgt
     8761 cgaaatggaa gccactggca gaaaagcttc tgggactgta aataccgctg aagattccat
     8821 gttggtgaca actattcctt atgacaaagg atggaaagct aaaattgatg ggaaaccagt
     8881 caaggtcagc aatttcaaag acgcattctt gatgataaaa gttccagcag gtaaacatac
     8941 tgtcactttc agttacttgc ctgaaggatt tacgataggt gcaatccttt ttgtcctatg
     9001 tattgcattg tttattcttt atgtctggta ccttgaccgc aatcgcccct tttatggcac
     9061 gattgatcca gcaatagaag aacaaccacg aaacacacac agaagaagac gtagaaaata
     9121 aagaaacgat ggaacctgtt cagactgtcc gcaccaaggg caatcaaaac aacgttccat
     9181 cgttttttgg ttacagaaaa aataggtaac aaaaagatga ttcacttatc aacgcctcgt
     9241 ccacaagtca cgatattcat caaagatgag aagctgtttt ttgaaattgc tctttgtgac
     9301 tcagagttaa atgacaggct cacgacctct ttcttaaacg gtgttcaatc agctgacctt
     9361 cggtattagc tcaacaaacg gcaaaatatg aggagctatt ttcccgtttg tttctctaat
     9421 actcaggtca aaacgctgat ttcacgacct cttccttaaa cggtgttcca gaagtagctt
     9481 cttcgaaaat aagccgaaat tttgcaaaat tttgagaagt aattttcgta aataccttct
     9541 tatttcttga agctgaccac ttctatcata acctcttatt catatttcac ttcatacaaa
     9601 taaagtcctt cagggtgtgc tgtaggtcct gctgcattac gatctttttt cgcaataata
     9661 tctgggatac tatcttcagg caatcggcca ttgccaattt tcaaaagcgt tcctactaag
     9721 atccgtatca tcttatacaa gaagccattt cccctaaatg taaacaacaa ttcgtctttt
     9781 gtttcattta cctccatctt agcctcgtag atcgtacgta ttttatcctc tatcgagctt
     9841 cctgatgcac aaaaagaggt gaaatcgtga gtccctaata aatcaggaag cgcccgctga
     9901 attttttcca aatcaagtgg gtaaggaaaa taacttgcat aatggcgtcg aaaaggactg
     9961 cgtggtttcc ctatatccac tcgaaattga tacgtctttt ctttgaccaa atatcttgcg
    10021 tggaagtctt ctgaaacaat ttctacttgt cttacagcaa tatcctctgg tgactgggta
    10081 tctagcgcga aacgcattcg ttctaacgga cgctcctctg gataatcaaa atggattact
    10141 tgcccgatcg catgtactcc ggcatctgtc cttcccgaac caaagaccgt gatttctttt
    10201 ccatttgcca ttttttgtaa tgttttttct acttcttctt gaacagtacg cccatttggc
    10261 tgtttttgga atccattaaa atttgttccg tcgtacgcaa tgattgcttt gtaacgtacc
    10321 atgtttttct ctcctttgtt cctaaaaggt tatctggaat aatgattttt ctataa
//
LOCUS       DAZKLA010000101        10126 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000101 DAZKLA010000000
VERSION     DAZKLA010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.102"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            268..537
                     /gene="rpsN"
                     /locus_tag="ACG85N_002180"
     CDS             268..537
                     /gene="rpsN"
                     /locus_tag="ACG85N_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0224905.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            552..731
                     /gene="rpmF"
                     /locus_tag="ACG85N_002181"
     CDS             552..731
                     /gene="rpmF"
                     /locus_tag="ACG85N_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0224906.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            763..1140
                     /locus_tag="ACG85N_002182"
     CDS             763..1140
                     /locus_tag="ACG85N_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224907.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1161..1310
                     /gene="rpmG"
                     /locus_tag="ACG85N_002183"
     CDS             1161..1310
                     /gene="rpmG"
                     /locus_tag="ACG85N_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0224908.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1334..2317
                     /locus_tag="ACG85N_002184"
     CDS             1334..2317
                     /locus_tag="ACG85N_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0224909.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2347..2469
                     /locus_tag="ACG85N_002185"
     CDS             2347..2469
                     /locus_tag="ACG85N_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0224910.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2487..4010
                     /locus_tag="ACG85N_002186"
     CDS             2487..4010
                     /locus_tag="ACG85N_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0224911.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4034..4273
                     /locus_tag="ACG85N_002187"
     CDS             4034..4273
                     /locus_tag="ACG85N_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0224912.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4466..4927)
                     /locus_tag="ACG85N_002188"
     CDS             complement(4466..4927)
                     /locus_tag="ACG85N_002188"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0224913.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5122..5340)
                     /locus_tag="ACG85N_002189"
     CDS             complement(5122..5340)
                     /locus_tag="ACG85N_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0224914.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5458..7074
                     /locus_tag="ACG85N_002190"
     CDS             5458..7074
                     /locus_tag="ACG85N_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0224915.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7315..8655)
                     /locus_tag="ACG85N_002191"
                     /pseudo
     CDS             complement(7315..8655)
                     /locus_tag="ACG85N_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9093..9239)
                     /locus_tag="ACG85N_002192"
     CDS             complement(9093..9239)
                     /locus_tag="ACG85N_002192"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224916.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9278..9472)
                     /locus_tag="ACG85N_002193"
     CDS             complement(9278..9472)
                     /locus_tag="ACG85N_002193"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0224917.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9382..9714)
                     /locus_tag="ACG85N_002194"
     CDS             complement(9382..9714)
                     /locus_tag="ACG85N_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0224918.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
BASE COUNT         3365 a         1608 c         2001 g         3152 t
ORIGIN      
        1 aagctactta aaacggtatt tgtgtttgat tggactaata aaagtgaatt cttatttttc
       61 agagcctaaa ctgcatattc ttttaaatga tttgattatc tctatgtacc gataaattcg
      121 ttttcaaata gttatctatt catatgagtt ttatttcatg ttatttattg ttgacaagag
      181 caactcttgt tggtaaagta tcagtgtcaa aacgtaacga ttacgatttg aggtgtctaa
      241 atcaaaataa aaggagtata aaggtatatg gcaaaaaagt cgaaaattgc taaagcaaag
      301 aaacaaatgg cgatgattga aaagtatgca gataaacgtc aggaattaaa agccgcagga
      361 gatcgcaccg ctctcgctaa attgccaaga gattcaaatc cgaatcgtct gaggcttcgt
      421 gatcagacgg atggtcgtcc aagagggtat atgcgaaagt tcggtatgtc ccgtattaag
      481 tttagagaac ttgcccatca agggttgatt cctggagtaa aaaaagcaag ttggtaaaag
      541 gaggaaatat catggcagta ccagcaagaa aaacatctaa ggcgaagaaa cggttaagaa
      601 gaactcatca aactgtagta aaaccagaaa tttcatttga cgaagcgaat ggtgattata
      661 gacgtagcca tcatgtatct ttaaaagggt actataatgg aaagaaagta attaaaggag
      721 cttcaaaata gtttaatata taacttttag gagggaatca aaatggaaat tatttaccca
      781 cctttagttg aagaaggatt aaagtattat cttgaaacga cccagcaatc gctagataaa
      841 tgcacttttt atcgttcgat ggtagagaga ggaattatta ctgaaacggg tttaccgacc
      901 caacaggcaa ttgaaaatgg attagtcaaa gattactatg aggaccaagg cttatcattt
      961 gatgattttt taagaatcta tccaattttt gaagagtatg acgaggagtt atttcaatgt
     1021 atcgatggtt attgggagat acctgttgat atgaaagaaa atttagtttc acaattggaa
     1081 tctggcgagt taacttttga agatgcacaa caaattcaag catatcttga agatcgataa
     1141 gtattgaagg aggaagattt atgcgtcaaa atattatttt ggaatgtgtc gaaactggag
     1201 aacgtctgta tctcaccagc aaaaataaac ggaataaccc tgaacggtta gaattaaaaa
     1261 agtattcgcc aaaattgcgc agacgggcaa tttttaaaga agtgaaatag ctaagtggta
     1321 aaaaggtgac aaaatgggca taccagttac aatcgtttct gggtttttag gggctggaaa
     1381 aacaacgcta atcaatcaag ccttgcaagt ctcaccctac cccagggaag aaattattat
     1441 aattgaaaat gagtttggtg aagttgggat tgatcacaaa ttattagttc atagcgagga
     1501 acgggtgtta caactaaata atggatgtat gtgttgcagt cttcgcagtg atttattagc
     1561 aatattgatt tctttactgg aaacaataga agaacatcaa caaccaattt cacaaatcat
     1621 cattgaaact acaggtatag cagatcccca accaatcatt caaacacttt taacagcgcc
     1681 tcaactaaga ggaagagtat atatcgatag tttattgact gttattgata gtgacagttt
     1741 tgaacttatt gaaagagaac cacaagccct taagcaactt gtcatggcag atcgcatttt
     1801 tgtgtctaga aaacaaaact tgaatttatc tgcactaaaa aaattacaga gagaaatcaa
     1861 aagtattaac cctttatccg atattcgaat gttcacacat agtgaaacga ttcaaaaaaa
     1921 ggatttttat aatttgaaaa aatttaatca tcccttagtt tttgaaagtg attcatcaga
     1981 aaatcactat aatggcaaat actatcatca acaccatcaa gtacatgagt ttaaatcaat
     2041 tctcttaact tcagatcaag acataaaaaa agatttttta ctccaatgga ttgactggct
     2101 gattttaacc aaccaaggga gcctgtatcg ttttaaagga ttgattgctt tacaagagca
     2161 ggattttata gttgccatgc aagaggtaaa cgaacaggtt aattttcaat acaccactcg
     2221 acaaaaagac caaaccacta ttattctaat cggaaaaatg ttggatgaag aaaggatcaa
     2281 acagagtttt gataaattac tggcggaaag caaataatga taagtagtga ttgggggtaa
     2341 tttctaatgg agaaaacgga tatatctagt gcatatcgac gacttaaaag cccgaatatt
     2401 aaaactagaa aaagggcatt aaaaattatc aaagaacata aacgaaataa aatgaagaaa
     2461 ttagcataat taaggaagga aaaagaatga agaaaatcag tgtaggactt attgggatag
     2521 cagctctagg attattggga gcttgctcca gcactaatga tgcaaaagta tcgaatgata
     2581 aagatggaaa attagaaatt gtaacaacat tctatccaat gtatgatttc accaaaaaca
     2641 tcgtcggcga tgaagcgaac gtggatttaa tggttccagc gggatcagaa cctcatgact
     2701 atgagccctc agctaaggat atggcaaaag ctcatgatgc tgatgttttt gtatatcata
     2761 acgaaaacat ggaatcatgg gtaccaaaag ctaaagaaag ttggaagaaa gctgggccta
     2821 atgtagtaga aggaacaaaa gacatgattt tattgccagg tagtgaagaa gaagatcatg
     2881 atcacggaga agaagatcac catcatgaac tagatcctca tacgtgggtt tcacccaaaa
     2941 tggcaattaa ggaagtttca aatatcaagg atcagttagt taaattgtat ccgaaaaaag
     3001 caaaagtatt tgagacaaat gctgaaaagt atttgactaa actgaaacgg ttggatgctg
     3061 actataccac tagtcttaaa gaagcgaaac aaaaaagttt tgtgacgcaa cacgcagcct
     3121 ttggatattt agctttggat tatggattga aacaagttcc tattgctgga ctaagtcctg
     3181 aagaagagcc atcttctggt cgattagctg aattaaaaga gtatgttaaa aaaaataaga
     3241 tcaattatat ttactttgaa aagaatgcta atgataaaat tgcaaaaact ttggccaacg
     3301 aagctgggat aaaattggaa gtactgaatc ctcttgaaag tcttacaaaa gaacaaatgg
     3361 ataatggtga agattatgtt tcagtaatgg aagacaatct aaaagcgtta gaaaaaacaa
     3421 ctatggttgc tggtgaagag gtagttccag aaaaagaagc gaaggatgaa aagacagttg
     3481 ctaatgggta ctttaaagat gccgatgtga aggatcgcga attgagtgat tatacaggcg
     3541 aatggcaatc tgtttatccg ttgttaaaag atggaatttt ggatgaagtt ttcgattaca
     3601 aagcaaaaat caataaagac atgacagctg ccgagtacaa agattattat actactggct
     3661 ataagacaga tattgatact atcaacatta aagacaacac cattgatttt gtagtcaacg
     3721 gtgaacatca tcaatacaca tacaaatatg ttggatacaa aattttgaat tatgaaaaag
     3781 gaaaccgtgg tgttcgtttt aattttgaaa ctgatgatgc aggtgctggt cgttttaaat
     3841 atattcaatt tagtgatcat ggaattgcac caagtaaggc agagcatttc catattttct
     3901 ttggtggaga aagtcaagaa aaactctata atgaattgca taattggcct actttctatc
     3961 cagcttcatt gtctaaacat gaaattgctc aagaaatgat ggctcattaa aattcaattt
     4021 ttggaggaaa tacatggtaa gaaaagtatt attgtctatg gattccaaga atattgatgc
     4081 ttatcggcgt tatcgatttg caaagcaaat gagattaaat gggagtacat tagctgatat
     4141 tgctgcaact ttacatgtta gtattgacac agttcataga gtattaaaac gggatcctga
     4201 ttgttgtttt atcaaggtca atactggaag aagtagacgt ttagcaaaac ggcataatag
     4261 cttggccaat taattgataa taccagagta gatcattttt cccttatttg gatttgatct
     4321 gctttttatt atgtcttgaa ttgtcaaatc aagcgcaaca ctttatcaaa gaagacttca
     4381 taaggtgttc tccaatctaa acactttcta ggtctgttat tcagtttttc aatcatctga
     4441 tttatcgttg agttatctac cgtagctatc tctttcgatt ttggcaagta ttctcgaatg
     4501 agtctattcg tattttaatt tgttcattgt tgccaagggc atgaggatct ggaaaataaa
     4561 agggcatatt attcaatgct tctgtaactg cgaaatgatt ggagaattct tttcctctat
     4621 ctggagtgat cgttttgacc gcctcatttg gaatactgct taaaagttca atcattttct
     4681 cgctcacata agcggctttc tttctgggga ccttctttcc tgctaaaagg aagcatgatt
     4741 ttctgtctgt caaagtcact aaacaagtac caccagtttt gccagctaca atatcggctt
     4801 tccaatgacc aaaatcactt ctatcatctg cggattttgg acggccatgg atcgaatgag
     4861 taaggcaaat ctttccacga gactcgacat gattttttgt atgtcttgtt tttcctcgat
     4921 ggtgcaatga acggatcagg cctttattgc cgtgtgagag gggttctgta tcgaaaagat
     4981 cattgcaaat tgcacgttag atcgtgataa aactaattga aaagtccata tgttcatgct
     5041 tcaaacgatt cgagatttcc tctggcgacc actgaaaatc gacaaataaa tgacgaacaa
     5101 ttgaccaaac tcacggatca tttagaatgt gataacgtcc gcattttttt cttcgataag
     5161 cataattttt ttccgctttg gaaggtgaat agtttttttc ttgtttcttc tcagttcatg
     5221 taaaatagta aacgggctat gctgaatgtc gttgccaatt atactaatag aaaatccttc
     5281 ttcgtgaagc agaaagattc tttctcgttc gagtatagta agatgacgat actggctcat
     5341 aattattttc cttctaggag tgttctcgac aaacttatac tagttggata tcgattatga
     5401 ctcattttta tgtgttgcac ttaaagtgta aattcaaggt atagaaagag gttccctatg
     5461 aaaaaaatac ctattacaat cttaactggc tatcttggtt caggaaaaac gatacttcta
     5521 aatcatattc tgtacggtga tcacggaaag gaaattgctg tcattgtaaa tgacataggg
     5581 gaagtaaatg ttgatggggt attagtcgac aagaaaggtt ttaaaagaac tggagaaaaa
     5641 cttgtttcga tgccgaatgg atgcatttgt tgtacattac gcgaagattt gattgttgaa
     5701 ctagatcaat tagtgcaatt ttctgacttg aatcaaattg ttattgaagc ttcaggaatc
     5761 agcaaaccaa ttccaattat tcaagcaatt atattagcta aaaacccttt agacattgat
     5821 ttgacccaac gattagagat tggtgccatt attatagtag tggatgtaaa acgtttttgg
     5881 gataatttcg aaacgggtga aaagattcag gaaagaaaag ctgaaggtaa tgtagttgaa
     5941 gaagaacagg atattcgtgg cttgttaatt gatcaattcg agtgttgtaa catattactt
     6001 ttaaataata gtgacttagt gtcaaccaag acgatttaca aaattaacgg tctaattgct
     6061 aagttacagt ccaatgccaa aataattagg acaattaaag gtgaagtaga cttatcgaaa
     6121 attctaaata caaatctttt taatatagat gagacttcct attcagtggg ctggttcaaa
     6181 gagttagagc ttggctatca aaatcatatt cctgaaacag aagaatatgg tatctcaagt
     6241 tttatctatc gaaatcggtt actccttgac gcacaaaaat ttgcccatct tacggataaa
     6301 aattttcctg gcaatattac aagagcaaaa gggtttcttt ggtttgccca acatagtgat
     6361 ttttcgacga tctttgaaac atcgggtagc tataaaagta ttgaatcatt aaattattgg
     6421 gtagctagtt tacctaaaat tgagtgtcaa atgattatac aaaaaaatcc cgaattgaag
     6481 gaagcctggc atccgttgtt tggtgaacgc attaataaac tagtttttat tggtatgaag
     6541 atggatcaac aatgtattaa ggaacaactt gagggttgtt taactgattc tcttaatgca
     6601 gataatttcc gaaaaatttc accatttcca tggaaaaagt ctaatatgtt tagccagata
     6661 tcgttaatga aatttttaag aaaagctggt gtcaagatag gcgatgtcat aattattggc
     6721 attttgatta ttacctcttt tattccctta tttgtattct ctatagaaaa ggagaatgaa
     6781 acaactgaag atgctatcaa atatgccatc gttcgaattg atggcaagga aatggatcgc
     6841 ttcgatttgg ataagatcaa tcataaactc gtgaattatc atcctgcgaa gggacaatat
     6901 aatattgttg agattaaaga tggaagaatt cgagttaaag aagataatag tcctgatcaa
     6961 atcgcggtta gaactggatg gatctcaaaa cccggtcaaa cgagtatttg tttaccccat
     7021 aagttagtta ttagtattga gaaaaaagaa agcacagatt actacattta ctaatctgga
     7081 aatagatgag gaggttttta ataagaaaaa gatattatta ttaataatag caggggcttt
     7141 attaagtgtg ttaagcgctt gtggacaaga aaaagaagcg gtgacaacag gcacaagtat
     7201 ctttaacggg acctgcaatg gtttagttaa cagtcggtta gattacttat tttggctctt
     7261 cgtcaatgag tgttggtgaa gaaattcaat agaataatcg agatactcac acggctagac
     7321 agctatcttg tctagccgtg ttgttgttct ggctttagtc agttgggtag tattcttagt
     7381 tttcttaaaa tattttcctc gatataaaaa gattcgttct cggtagtttt gaaagtttct
     7441 atagccatag gcaattcgtt ttagtacttt gatatggtta ttcaaacatt ctatagggcc
     7501 gttggaatag ggaacgcgca acgctcgttt gatttctgtt tggtatttct tgaaggtcgt
     7561 gaacactggt tggtagtgct ctggcaactc cttgtagtct tcttttagga gcgcgacgaa
     7621 ttcaggaaca tcttgtcttc tgataacgga aagaaaatcc tggtagactt cgtagcctcg
     7681 ctttaaggaa tcatcataag ataatagccg atcgactatc tctgcttctg tcagatgatc
     7741 tcgaaatgac ggacgccagt agcgatgctg acctttcaat tcccacgcat tcttttgaag
     7801 tagtttccag tatttcttta agtgcttcga ccgacgatcc ccttttttaa aggtcttcat
     7861 tacttgaatt cggtggttgg taaaagcccg accaatgtgt tggctaatat gaaagcgatc
     7921 ggcgacgatt cgggcattcg gaaaacactc tttcaccaat gtgcgatagg gtgtatagaa
     7981 atctgaaacc actagacgca cttgttcacg gacttttata gggtagcgca agaaatagtc
     8041 gcgtaaagga ttgagttgcc gattttccac aatatcaatc agttgatggg tcttgccgtc
     8101 catcatgaca aagctcatgg ctcccgagac atttttcact gatttgaatt catcgaaaca
     8161 caccacttct ggtaacagct ggctcaaaga aatggtttta ggccgaaggc tgcgaagtat
     8221 tcgaatgaca gtggtcggag aaatgtgctt catacgtgcg atcaaagaca tagaaagagg
     8281 ttcgcttaat agctcaagaa tggcttgttt gactctgcga gcaatggagc agcgacgatc
     8341 actaactgaa tcttctgcta aaaaagtctt tccacagtgt ttacatttga agcgctgttt
     8401 gttaatccgc agaatggtct tgtagttact gacatcattc aataagatgg atactttttt
     8461 ccagccctat ttaatgatcg tttgttcatt ttgatagtgg cgatgaaagc attcctccgg
     8521 ttgataggtc aatgtgccat caaaaacaaa acatgtttcg tcattgattc gctctttgcg
     8581 aaaggcatct tcatgaaaga taagatttag gtctagaata tctagtaact ctttggtaaa
     8641 ctctgaatag gacatcttga aacactcctt tgaatggttt tcgtcgactt tattctaaag
     8701 gattttaagg tgtctgtttg cgtttattag aaaaaagaaa aagtattggc aacagaaggc
     8761 gtcaccaaca ctagatatta tagatccctt attttcttcg cttcaaattg tcaaggagtc
     8821 atcataaatt ttgtatggat tcttttgttt gggtagtagt cttctaaata atcttggatt
     8881 aacaccttca tctctatttt acttttatat ttgttggagt cgattaattc tcatttgatt
     8941 gatcgatgaa acgcttcaat tactgcattg tcaacaggag tcccagtttg gctcactgaa
     9001 tgtgtgatgt ggtggtcgtt caacagctct tgataaataa aagaccgatg ctggctacct
     9061 ctatcggaat gaattaccgt aggcaattct ccttaataaa tttgtaatgg acggacgaca
     9121 atctcttttt tcatgtgtga atctaccata tagtaacgta gaatacgagt tgctaagtca
     9181 atataggtgg acaaatagag gcgaccgtca ttacatggaa tgtaagtaat atccatcacc
     9241 caaacagcat caatttggtt ctgttcaaat tatcgattca acattttttt accatttctt
     9301 tgattgtttt ttctctacca tgagaagatt tgcgtcgatg aaatcctctt gtgcataaat
     9361 tcatttcacg cataagaaca gctacaacac gtttattgat ttcgattcct tcatcgtaaa
     9421 gtttttgagt gatccctgga gatccgtatc gatctttgtg ttcgtagaac actcgtcttc
     9481 ctcttttagt cgtttatttt cctgttcaag aatgtagaaa tctgctttat caggtaatct
     9541 ttttccttgt ccagcaaaag catttttccc gtatcgacga tattctgaaa cccatcggga
     9601 aattgttgga cgagagatat tgtattcttc agcaattttc ttggtagtta cttttttctc
     9661 aaggatatcc tgtgaaatct ttagtttaaa tttacgatca tatgtttttc tcaatagagt
     9721 tcattccttc cggtaacgta actgatagtc aagaaaagtg tatcatagcc caacttagta
     9781 tcagggtttg tcaactaaac tgtggaagtt gatagataag agtttttaac aaccaaaatt
     9841 ctcttgacta tttttgaaat cagacaaatt ttgattggac acatagttga ataaatctac
     9901 cgtcgttacg gaaggtaaat cgcatgcttt tcaattctag aggagaagtc ctcgttgaag
     9961 taccatagcc cagttctgga ttcgacctcc tgcccacttc atacgtaaat aaagtaattt
    10021 taggagtgag actgtgacaa aagttatttt taagctaaaa acgaattaaa aaacgttgat
    10081 aattaaaatc attcgtttta gattctatga atcattatag aacgct
//
LOCUS       DAZKLA010000102         9919 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000102 DAZKLA010000000
VERSION     DAZKLA010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.103"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            254..733
                     /locus_tag="ACG85N_002195"
     CDS             254..733
                     /locus_tag="ACG85N_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFQ0224919.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            766..2994
                     /locus_tag="ACG85N_002196"
     CDS             766..2994
                     /locus_tag="ACG85N_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0224920.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3123..4766
                     /locus_tag="ACG85N_002197"
     CDS             3123..4766
                     /locus_tag="ACG85N_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0224921.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4839..5384)
                     /locus_tag="ACG85N_002198"
     CDS             complement(4839..5384)
                     /locus_tag="ACG85N_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFQ0224922.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5407..5892)
                     /locus_tag="ACG85N_002199"
     CDS             complement(5407..5892)
                     /locus_tag="ACG85N_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0224923.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5882..6694)
                     /locus_tag="ACG85N_002200"
     CDS             complement(5882..6694)
                     /locus_tag="ACG85N_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFQ0224924.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6833..8365)
                     /locus_tag="ACG85N_002201"
     CDS             complement(6833..8365)
                     /locus_tag="ACG85N_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFQ0224925.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8474..9505)
                     /locus_tag="ACG85N_002202"
     CDS             complement(8474..9505)
                     /locus_tag="ACG85N_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0224926.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3306 a         1840 c         1908 g         2865 t
ORIGIN      
        1 acaaaaaaga tgttggtgaa gttcaccaac atcaaaaagt atagagccaa aatattccaa
       61 catcatcttg gactttctac atgttactaa tgagcgtgta aaagaacgag atggtgtttt
      121 tatttctatc aatgaagctc tttttctttg caatcctcac ttcaatcctc tataatgtcg
      181 catatagttt agtaaatgag taaactacta atttacttat ttactaaaaa taaaaggatg
      241 aggagttaaa ataatggaaa taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga
      301 agactatgat aaagttgatg gaagaaatgc attaaatatg gatgcaaaag ggctttcact
      361 gggccttgct cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca
      421 tactggagaa aaatggtcac gacagtcgga agaattacca atcacacgta ttttagattt
      481 agcgatctta gctgcgcaag gaagtttata cttccaagat gcttatcgtt atgaaaaatt
      541 ttatgatcca gaaaatccag tcgtagacat cattggatta caagggaacc gaatgacggt
      601 agaagtagat accaaaaatc caaagatcga tgaagacatc attttgtatt acgatacact
      661 acaaaaagac ggagaactga ttggggaacg attccgtatt ttaaaacgtc ttttagatga
      721 gctaggatat taatagacag agatgatcaa aaggggaaga gaagcatgga gaatttctca
      781 ctaacagaaa accgaacctt ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa
      841 agccatcgga caagtgcaga agaatggcgg atcgcctccg aaatcaaagg gaattggcaa
      901 gatcctgaaa tgaaaatcag taacgtactt ttggaaggag atgccgggtc ggggaagaca
      961 caattagcca aagcactatc tgctgatctg caacttccct atacaaaagt gacctgtttt
     1021 gcagatatgg acaaatcaga tgttttcgga gccttattac ctgtagtaga ttctaataag
     1081 gaacaagacc aagaattact agaagctatt taccaaacag atacacttga agctgtactg
     1141 acactgatcc agaaccatta cggcattgat cctttttctg ctaaagaaaa attagcagag
     1201 ctagttcaaa ggatcgaagc tgattatcca gatgcaatcc attacagcta ttacccttct
     1261 gaaatcgttc gagcgattga aaaaggctat ttattagaga ttcaagagcc gactgtcatc
     1321 cgagatgcct ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat
     1381 ttaccgacag gaatcattcg tcgccatcca gattgcgtca ttgtgatcac tacaaatcga
     1441 aattaccaag gaaatcgccc attaaatgaa tctttaagag atcggatgca acatgcggaa
     1501 aaaatggatc ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa aactatgatc
     1561 caaaaacaag aacttctctt aaagatggca gagattatcc gtttgttgga tgaaactgca
     1621 aaagctaacg ccatcaaagg tgtagcagga atgcgttctt atttttattg ggcaaatacc
     1681 tataagcaag gacaagattt actccagtcg atttatccaa aagttttgta taaattatcc
     1741 actgattcag atgaattggc aattcttgaa caagctttgt ctgacagtgg attgctactg
     1801 gaacttcagg acatattgag aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa
     1861 gaaagaacca ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa
     1921 attctgcgct tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga gggagaagag
     1981 aaacaagaag aaacaataga aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt
     2041 agcgatatcg aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc tcaagaaatg
     2101 tctgcttctg acatggaagc acatgataag gaaatcaaga agcaactgaa taaagaagcg
     2161 cgacaaatca tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca ccgaccagct
     2221 ttccaaggaa aaacgccgga agcacgtgag ctacacatgc aagtaatgcc aatagtagaa
     2281 tcacttagcc ggcaaatttt agagttactg gataatgaac aaacagagac gtaccaaaaa
     2341 ggaaaatacg aaggtcagcg gttcaatgca agcaaagtag catatggtga tttgagaaat
     2401 tttgacaaaa agaacccgcc acatgagcaa ccttctttag cggtagctgt acggatcgat
     2461 gaatctgggt caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc
     2521 attgccgaat tttctaagaa agtcgatatc ccattgttga tttacggaga tacagcagac
     2581 cgttcgtcaa gagaaaagac ttctttgttt tcttataaag aatttgaaga cggatttcaa
     2641 tggttaaatg aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca
     2701 cttcgtttag cagcagaaaa attaaacaga caatccgcaa caaccaaact gttgcttaat
     2761 attagtgacg gtcagccaaa agcgttgccg gattatacag gcaaaaaagc aaaagaagat
     2821 atccagtctg ttttgaaaga atatgaacgt caaggaatcc tatttgtctc cgcagctatt
     2881 ggccaagaca aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt agatattact
     2941 gacttgaatg aatttcccaa gcaaatgatt caactgattg cccgctatct atgataaaat
     3001 aaagctcaac gaacgcactt agcagattcg ttgagctttt cgtttctagt taccggtaac
     3061 aaggatactt ctaaaataga atcggttata atcaagaggg accgttaaag gagtgaaacg
     3121 atatgataca agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc tatcctgcta
     3181 gtttcaaaga tagcaatggc gatggtcaag gagacatcaa tgggatccgt gaaaaactta
     3241 attatttgaa agatcttggt atcggcttta tctggatcaa tccgatctat caatctccat
     3301 ttgtcgataa tggttatgac gtaagtgact atcaagatat agaagagaga ttcggaacga
     3361 tggaagattt tgatttattg ctaaaagaag cacatgaatt agggatcaaa gtgattatgg
     3421 atcttgtcat caaccatagt tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta
     3481 aagaaagccc gtatcgcgat tattacattt gggtcgacgg ggtagatggc aaagaaccga
     3541 ataattggac atcgattttc ggcggttctg cttgggagta ttctcatgaa actggacagt
     3601 attatctgca tgtttttgca aaagaacaac cagacttaaa ttgggaaagc gagaaattga
     3661 aagaagaact attcaatatg atccgatggt ggttggataa agggatagat ggatttcgtt
     3721 tagatgcgat ttctcatgta aaaaaagatg aatattcagt aaaagccaca gagaaccctt
     3781 tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg aaacatgttt
     3841 ttgaagagta cgatatcatg actgtcggag aagcaagtgg tgtcactgca gaagaaggac
     3901 cacaatgggt agggaaagac ggttatttcg atatgatatt cgaatttgat catatccata
     3961 tctggcagca agaaaaagaa gggcaattag atgtccttaa attgaaacat gcattaagcg
     4021 cttggcaaac ttcattggat ggtatcggat ggaacgcttt atatatggaa aaccatgatg
     4081 ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga tttttgggct atgtctgcaa
     4141 aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc atttatttat caaggacagg
     4201 aaatcggaat gacgaatatg ccctttgaat cgatcgatca agttgatgca gtagattcga
     4261 aaagacttta taaaaggctg cttgcggaag gtaagacaag agaagaagca ttggatatca
     4321 ttcgtgaaac aacgagagac aacagcagga cacccatgca atggacttcg gaacaatatg
     4381 ctggtttttc tacacatgaa ccttggctga tcactaatcc aaataccaaa acgatcaatg
     4441 tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt ctataagaac atgatccgca
     4501 ttagacaaac caataaaggt ctgatatacg gttcttataa agaatatctt catgaacatc
     4561 cacaacttta tgtctatgaa agatatttag aagatgaaca atatcttatt atggtaaact
     4621 taacagaatc acttgctgac tatgaacttc caaaagaagc agatcagtcc tggacattac
     4681 tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg catactagca ccttatgaag
     4741 ctcggttata taaaacaaac aagtaaaact tcctcgttaa agttcattag ttggactttg
     4801 cgaggaagtt tttcagattg ctctgtcaat tactcatttc aataattcgc tctaggacca
     4861 ccgatatatt taggacgaga gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa
     4921 cgaggtctga caggtacttg gacatgcttg atatgcatac cgattgatgt gtcaccgata
     4981 tcgataccgg cttgtccaac tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa
     5041 gcagcaacag aacaagcacc tccagcatgt agtgcaggaa caacagagac aatttcgaaa
     5101 ttttttgctt ctgccactgt tcgttctact actaatgctc ggttcaaatg ttcacaacct
     5161 tggacagcta gcgcaatttc tttaggatcc aacaactctt ttaacgtacg gatgatccat
     5221 tgaccaactt ccgcactgga attttttcca atatgtcctc caacgacttc gcttgtgctg
     5281 catcctaaaa caaaaagatc accttttttc aagtgtgcct cagccaatat gtcattaact
     5341 atttcagtca attggtcttt taattctttt tctgacaact tcattttcta aaaccttctt
     5401 tttcaattaa tattgcttcc gtgcttgaat acgttgaaga cttgcagtta taggaaaggc
     5461 aagtcccatt ccaatcagat tttggaagat gtttcctggt atagaagcca ctccagccgg
     5521 ccaatcatat aaaatccaac cggcgatacc atagccgatg atcataacta agcttcctaa
     5581 taaaagccct aatccccaca tacgagtaga agatttgctg aaagaaccag caacatatcc
     5641 ttgtaacccg tggatcacta atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc
     5701 gatcaataga ccactggaag caccaacaac taatccaccc gttggaccat aaagaaaagc
     5761 agcggtatag atcccggcct cgcataatgt cacaaatcca ttcgtagccg gtacagggat
     5821 caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc atagcataga tcgttaattg
     5881 cttagttttc atttctcatt cctccttttt gaataccgcc agaccagaca cttccggtat
     5941 gatcgttcat cagcacgcca ttagaaatac aatgggcaac aaatttttta ctttctgcta
     6001 ctgctttggt aaaatctgcc cctaacacaa gattcgctgt tattgcagaa gaaaaggcac
     6061 aaccggcccc gtttactgtt gtagtgtcga tttttttatg agaaaatacc tcataatgat
     6121 caattgaata gagaagatcc acagcttcat ctccagccat ccttgtgccg cctttgatca
     6181 caatgttttt tgttcctgaa gcagtgagtt ctttagcgag atggatcatt tctgttaacg
     6241 aagagggaag agcttgacca gaaagtaaag cagcttctgc caaattaggc gtaatcatcg
     6301 taacaagtgg aaaaagttct tcgatgagct gcgaaatata caattgattg atcacagctt
     6361 cagtttcttt gaatgccaaa acagggtcta aaatgattgg gatattttgt tttgttttta
     6421 tgaagtctcg aacaaccatg attgcttcac ttgaattcaa taacccaatt ttgataccat
     6481 ctaatgaagt cattttatca atcgaaacta actgtcgttt aagcaattcc aaagatatag
     6541 attccagcat aaatcgctca ttaatatcta gtgcccctat agaagtcaag gcgcttagtc
     6601 cgaatagacc aaaattctca aaagttttta aatctgtttg gattcctcca ccagcaaatg
     6661 gatcagaacc gccaattgtc aagattcttt tcataacatt ttcctccatg aataagagta
     6721 taacgaaatt ttcaaaaaag aaaaacagcc aattggcata tttttatctc atccaattta
     6781 aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct ttccttaaat agttaggact
     6841 tttgtgattt taattctttc tcgatttgaa gccgttgcac tttcattgta gctgttcgcg
     6901 ggattgcatc aaaatcacgg atgataggga cgttcaagcg aggaagttca tggacttgct
     6961 cccaccatcg attccaatcc atttctttat caggagctaa agcaataatc ggttgtggag
     7021 aatgattttt atcccttaca atcactactt ctgccaaaaa atctaacgaa tctaataaaa
     7081 agtcttcaat agctaatgta ctattgatcg tttcgatcaa atccacttgc cgatctttca
     7141 aaaataggtg gccatgctta tccataaaac cgtaatctcc gctatcccac caatcgccat
     7201 aaacattttc ctggaaccgt gcatcttcct tgtaataagt caaagcccgt cctttagaca
     7261 gtaattgtat atgaccatct gtgttttcag gtaggacatt cccaactgaa tctgtaatcc
     7321 gagcgcttgt catgtcttcc agtccaacgc ccatatctct tgcatcactt gttttcaaac
     7381 tctctagtgt atgagcctta agaatcattg gtccgcattc actttggcca tatacttgaa
     7441 gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc tggttgttta
     7501 tagcatcaaa agtggagtga tagtatcgga tacccgaaaa agcttctgga tgctccttag
     7561 cagtaaaacg ccactgaaca aagttattcg gatgggtctc tagtgcaatt ggccggtgag
     7621 cttccaacat atgaacaact tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc
     7681 ccatggacat caaagaagag ataccaatat tgaatctgga atgcactgga gagatgtgaa
     7741 aagcgattaa tttcttttcc gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc
     7801 atcccataga atgagcagaa tgacaaataa gtttagggat accagttgta ccagaagtat
     7861 gggtcatata agaaatttca tttttgtcta aaaatgattg agaaacagac aaagcaggtt
     7921 gttccattaa ataaagaata ggaatctgtt ttgattttgt tccatttgaa atttctctca
     7981 ctcgcttttt tgtctcatca tcaaataaaa tgaaagggtc ttctaaacga tcgataaata
     8041 cttccattgt tgttgttggt agatgataag aagtcatgac aggcactgca ccaaggtaag
     8101 acacagaaac agctagaagg tatgtgtcaa aagcggaact cttataaata ataactttgt
     8161 ctccggattt cactccaaga tgagccaatt gataagcccg tttcaacaca ctttcgcagc
     8221 attttttgta agttgtttca agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga
     8281 taatcggggt ctcggaaaag ttagctgcag cttcttgaaa attcgtaaat aagttcaaag
     8341 gggaatacgt tatggtctcc atcattcaca ctccattatc ttttgttaag taatatacaa
     8401 attcaagata atcgtaacat aatcctttat ataataacat agtttattgt gaaaaaaaga
     8461 tcatacattt gttttatatg gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa
     8521 aatcaaccat cttttcccag atatcttgaa aagaatggga tgagaggtat cgattaccac
     8581 ataaataatt taccgcgtca gcatcatcta aatatagttc tgcataataa cgatatgttt
     8641 ttctgcatag ttctggcgat acgtttttat atactgtaat tttgctcaaa atttggcgaa
     8701 tagtccgagg agaaatttgc tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg
     8761 tattttgtgc tgttaactta aaatagttct ccagaaatgg catcgcttct tttggtattg
     8821 gtactttatt cagtctgttt tcttttctat tcagattaag aatctgcttg gtcaaagata
     8881 catctttacg tttaagttct actacttcat ggatctttaa tccgcaatac agaagaatgg
     8941 cagtaattgc aaggttcctc ggtctattct tttcttcaaa gctagcttct ctgcttgttt
     9001 gaaagccttc aatcgggata aacaaatgct caaaatcttt gaaaagttct gcatcgatat
     9061 atcgcggagc tgctttggtt gtttccggta aacttgtacc caaaagagaa atctcttctt
     9121 caatcagtcc tttttgatac aaaaatcgta aaaaatgatt taatgaatat tgcttccttg
     9181 aaatcccgta atagctattt tcaaacaatt gctccgtttc ttggttcgct gtagtttgga
     9241 acgaacgagt atgtcgatag attttagcta agaaattctg ataatcttgt ttttctaaat
     9301 ggataatcag ctgatcatcc acttcttcag aaaatacttt tctcaaataa tcagtaaaca
     9361 gatgaaaatc ttttgcgtat tcatagcgac tgcctaatga aagggaagaa cgattttctt
     9421 ctaaaaactg ttgaaagacg gaagggagca agtgtgtacg ttgctttaac ttttcttgca
     9481 gttgttttct agttttgctg tccattacaa attccctcca atagtgttca ttttctatat
     9541 tttaccgcat ttcatcaaat gaagatagac agtttgtctt gatcttagga agcaaatttt
     9601 taactaagta ctagctaaaa gatatgaagt aagaagatac atttttagtt attttattag
     9661 tgcagtcatt cttttgatct tttaattgat ttatcctctt tttagctatg tggttacttc
     9721 atgagcaatg aaatggattt ggttcgttct aatggcagga ggctctttct tttatttccg
     9781 aaaaattaga aaacattttt tcagaatcga ttggctctcc atttattcaa taatggtttg
     9841 tctaacgata ttgttgggat aattcttaaa gaagtattag aaatagttgg tacttcatta
     9901 ctttacatga agatttatt
//
LOCUS       DAZKLA010000103         9798 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000103 DAZKLA010000000
VERSION     DAZKLA010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.104"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..2154)
                     /locus_tag="ACG85N_002203"
     CDS             complement(34..2154)
                     /locus_tag="ACG85N_002203"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0224927.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="ACG85N_002204"
     CDS             2384..3562
                     /locus_tag="ACG85N_002204"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFQ0224928.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="ACG85N_002205"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="ACG85N_002205"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFQ0224929.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="ACG85N_002206"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="ACG85N_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFQ0224930.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="ACG85N_002207"
     CDS             complement(4915..6168)
                     /locus_tag="ACG85N_002207"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0224931.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="ACG85N_002208"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="ACG85N_002208"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFQ0224932.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="ACG85N_002209"
     CDS             7746..9092
                     /locus_tag="ACG85N_002209"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFQ0224933.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9387..9773)
                     /locus_tag="ACG85N_002210"
     CDS             complement(9387..9773)
                     /locus_tag="ACG85N_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0224934.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3126 a         1777 c         2007 g         2888 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttttctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgttt gtatagaaat
     9241 cgattgattg ccttgctctt tagtttgttt taggacttta agaacagtga ggctgacttc
     9301 gaaggttaat tctgtttttt tgttttctag aatcagtaag caaaaaagca ctcacttcga
     9361 gtgctttttt gcgatatatt cttagcttaa gataggccta atttttcttt tacagatgag
     9421 ggtaccttat cgctggtaat tacttttttc tctatgatcc gcttttcgct tgctttcaac
     9481 tgcaagatcg ttccttcggg gtaagcagaa ccattggcag agtcaccaga agtaatgaca
     9541 tcgaaatcta actgtctttt ttcaccgttt tcagcataag catctacgtt gtatttaaaa
     9601 cctcttccaa ttacttcccc attatctgct ttgatatcag tttctgcagg cattggagct
     9661 ttgactaccg cataataatc tttacctaca taagtagact gatagtagtc ccaacctttc
     9721 catcctataa aagcaatcaa tatgataacg atcaacccta aaattttttt catgaaagat
     9781 ctcctttttt atttttat
//
LOCUS       DAZKLA010000104         9786 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000104 DAZKLA010000000
VERSION     DAZKLA010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.105"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(154..1221)
                     /locus_tag="ACG85N_002211"
     CDS             complement(154..1221)
                     /locus_tag="ACG85N_002211"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0224935.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(1241..1720)
                     /gene="pssE"
                     /locus_tag="ACG85N_002212"
     CDS             complement(1241..1720)
                     /gene="pssE"
                     /locus_tag="ACG85N_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssE/Cps14G family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFQ0224936.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(1737..2198)
                     /gene="pssD"
                     /locus_tag="ACG85N_002213"
     CDS             complement(1737..2198)
                     /gene="pssD"
                     /locus_tag="ACG85N_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssD/Cps14F family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFQ0224937.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(2212..3462)
                     /locus_tag="ACG85N_002214"
     CDS             complement(2212..3462)
                     /locus_tag="ACG85N_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFQ0224938.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(3840..5291)
                     /locus_tag="ACG85N_002215"
     CDS             complement(3840..5291)
                     /locus_tag="ACG85N_002215"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0224939.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(5612..6376)
                     /locus_tag="ACG85N_002216"
     CDS             complement(5612..6376)
                     /locus_tag="ACG85N_002216"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFQ0224940.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(6404..7102)
                     /locus_tag="ACG85N_002217"
     CDS             complement(6404..7102)
                     /locus_tag="ACG85N_002217"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFQ0224941.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(7114..7893)
                     /locus_tag="ACG85N_002218"
     CDS             complement(7114..7893)
                     /locus_tag="ACG85N_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFQ0224942.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(7909..8853)
                     /locus_tag="ACG85N_002219"
     CDS             complement(7909..8853)
                     /locus_tag="ACG85N_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0224943.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(8899..9678)
                     /locus_tag="ACG85N_002220"
     CDS             complement(8899..9678)
                     /locus_tag="ACG85N_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224944.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         2816 a         2044 c         1491 g         3435 t
ORIGIN      
        1 ctcttgctct attacaagca ctaaaacact actattcatt tgaattctta cacggataaa
       61 aatttacatc gtctatttca atattttttc tctctatttt attcacttaa taagttcatt
      121 acataatcga aacatttttt attttccttg tcatcactcc ttgctccctt tttgatacag
      181 atttaaaatt ctatcaaaga aatttccatc gttaaatttc gttattgcca aatccttcga
      241 acaacgtgaa aattttacgt atttatcttt atcattgatt tttagtattg cagatgtcaa
      301 cgctgatacg tcaccaggtt gaaacaaata tccattttct ccatcacgta ctgctgaaga
      361 aatatctcct acatctgtag caattacagg cattccataa gaaatcgcct ctagaataga
      421 tataggcaat ccctcgttat aggacggtaa aatcatcaca tttgatcttc tatagcactc
      481 ctcctttttt tctcccacaa cccaacctgt aaattcaaaa tacgattgca aattatattc
      541 ttctatcaat tctttgagcg atatttcttc atcacctgct ccagcaatct caaaaataaa
      601 ctttttttct ggatattgtt ctgcaatgtt tttaactgct tctaccaaat cgtgtacacc
      661 ttttcgcttt atcaaaactc ccataaacag aaatcggcac atttttgaat tccactttat
      721 ggtatattct ggaactacaa tagaattttc tacaacaaca gttttacaat ttggctcaat
      781 ttctttaatt atcttcttcc atttttctcc aagtacaatc gtggaatcag actctcttaa
      841 cagcaagcga atctgatttt gtttttttac agaaacacta tcataccatt ttttaaattc
      901 cgatccatga agatggatga ttacaggaat atttaataat ctaaaccact tattcagcac
      961 atatttccta ctaaaactcc ctctgtacga catgtgcata tgaataatat taggtttact
     1021 caataaagag atacctataa ttttagcaaa tgcatacata aaatataaag atttaaatat
     1081 tgcatttcca cccttataac tcggtataaa agtcatctcg atattctttt tttcccaatt
     1141 aaaattgagc atttgtgtaa ttatacttgt aattccacct tttaccgagc tgtcattacc
     1201 aatcataagt actctttgca taacgcttta ctccttatat ttatattttt tctattaata
     1261 cctctatcga attaataatc gtttccgtgt tagatacata cttgttaaac atccgaactc
     1321 ttgtttcttt aacggcatct tctaaatttt ctaattcata acaaggatag ataatattca
     1381 tctcatcaaa ttgtctaatt aactgcagtt ggtgatcatc gacatgctca ccatacttag
     1441 ccaatcgagg aacagcaatg acccttttac ctttttttac ggccccaatt atagcacctg
     1501 tacctccatg agtaatcacc atttctgctt tttgcatagt ttcagtaaac tcatccctat
     1561 ttagaaatgc tttataggaa taatttagtg gtttataatc actataacca ctctgtgcaa
     1621 atgtactttc gattattact cctttttcaa ttaaatcatc aatagctttt aacaatctat
     1681 caaattgaaa tttttgtgaa cctaatgtta taaatatcat ttttttctcc tattatttaa
     1741 tatatcccac ccaaataaat agcattagga taaaattgta gcatagtttc ccactgaaca
     1801 taaaattgat ctgcaaaatg atacatcaat tttcctgtct gagtagggct ggttactttt
     1861 gcaaacgatt caatataaat cagttttttt cccatcattt ttgttattaa gcagataggg
     1921 atcatagcta aaacacctgt acaaatcacg agatccggtt tttcctttat atatataata
     1981 attgatttga ataaattaat aagtatagta cacaaaaaaa acttttcttt tctatttact
     2041 tgtctcatgt agtacgtttt ttcgcctttt gactcagctg aatattctgt ctgttctgtt
     2101 ataacaaaac tctcatactt ctccatcagt ggttttaaca tcattaactg ttcataatgc
     2161 cccccagagg aagccgcaaa acatatcttt ttgttcatat taacctctct atcagtcacg
     2221 ctgaaaaata tctctagtgt aaactttttc tttaatatca tccaagcagg tatcatatcg
     2281 attggcaata atagaatcac acatcgcctt aaactgctct aaatcgttga taaccttact
     2341 tccgaaaaaa gtctctccat ctttcagtgc aggctcataa atcaccacag tcgcaccttt
     2401 agcttttacg cgcttcataa ctccctggat tgaactctga cgaaaattat cactgttgct
     2461 cttcatggtc agccggtata taccgattaa aactggattt tccctgttga catcatactg
     2521 attatattca gcgtagttgt aatagcccgc catctgaaga acacgatctg cgatgaaatc
     2581 cttacgggta cgattagatt caacgatagc ctcaatcaaa tttccaggaa catctgcata
     2641 gtttgccaac aactgcttgg tatcttttgg caagcaataa cctccatatc caaaggatgg
     2701 attgttgtag tgagttccaa tacgaggatc gagacaaaca ccattgatta tctgctgagt
     2761 attcagcccc ttcatttcag cataagtgtc taattcattg aaatagctga ctcgcagtgc
     2821 taaataggta tttgcaaaca gctttactgc ttcggcttca gtgaatccca taaaaagagt
     2881 atccacgttc tccttaattg cacttttctg cagaagtgtt gcaaaaacat gagcagcatt
     2941 taacaagcgc tcatcagtca tatcagtacc aacaataata cgagatggat acagattatc
     3001 atataaagcc ttgctctcgc gcagaaactc tggactaaaa ataatgttct tactccccgt
     3061 tgtctcacgg actgattcag tgtagccaac aggaattgta gacttgatga ccatgatggt
     3121 atcaggattc acgtccatca caacctcaat aactgactca acagcagaag tatcgaaatg
     3181 ctgagtacga ctgtcatagt tagtcggtgt agcaataaca acgaaatctg cggatttata
     3241 agcgctcctt gcatccaaag tagcagttaa gtcaagttct ttttctgtaa gatacttttc
     3301 aatatattca tcctgaattg gagacttccg tttattaatc agttcaatct tttcagggat
     3361 aatgtctacc gcggttacct ggtgatgctg cgccaatagt gtggcgatac tcaaaccaac
     3421 ataaccggtg ccggctactg cgatgttgta ctttttcttc acaatatctc ctctttcctc
     3481 tcttaagtat ggagaatgtt actcctacgg ctattcatca tgtcaaaata acacagctta
     3541 gaatgttgat aaaataattt tccagaatgt gataaaatct ctttcattta taaaccacac
     3601 atctaatttt atctattctc cacaagacat gtagagtatt ctttaataac aagatacgac
     3661 cttactaaat acttcaaaaa attatcatat ttaattcata aatataaatc ttcattttaa
     3721 atgtatgccc aaaaatttat ctgtaatgcg tcatcaaaat ctagattcca aatctttatc
     3781 aatttttatc cactcattct accactttag taatattttc tatttataac actttcgtcc
     3841 tacattgatc cccccctctg aagtacagtt tttactgtct taaataaaat tttaaaatct
     3901 aaccccatac tccaattttt tatatattcc gtgtcaagtt ttgctacttc ttcaaaatca
     3961 gtaatatcac ttctaccact tacctgccac attccagtaa ttcctggctt aatagcaatt
     4021 cttgcacgat gatgataatc atattcttca tactctgatt taaggcatgg tcgaaatcct
     4081 ataaggctca tctttcctgt tagaacgttg aaaaattgag ggaattcatc taaagacgtg
     4141 gatctaataa attgaccgat tcctgtcttt tttgttccat ctggaagtat tttatttccg
     4201 ataattcttg gatcaaactc catcttaaac ataagattat tgctcatctt attttctttc
     4261 attaattcta cttttctttc ttctgcatcc atatacatac ttctaaactt atacatcttg
     4321 aacggtttac cattctgacc tatacgttct tgtgcaaaga aaattgggcc tggtgaatta
     4381 atataaataa gtggagcaat aaagataaaa ataattcccg ttgcaataca tccaacaata
     4441 cctgcaagaa tatcaattgt tcgttttata aatgcctgtt gagttgtcat aacattgata
     4501 cttgttgtaa gtactgtata ttctcctata cgaccaatag attgttttcc caaagcacta
     4561 tctgatactt ttgcaagatt caaatgtaca gtaaggccca tttcagagca tcgttcaata
     4621 aattctttca tacacgaagt acttttattc aaaataataa agacttcatc tacccatccc
     4681 tgacataagt aatccgctgc atttttggaa tcagaaacaa ctgtcactcc ctcaacatct
     4741 tcaccaatca tgcttttatc tatgataatg acaccattta ttttatacat tttatagtta
     4801 tgctctttca cattacgaat aactacttca gcaatactac tagatgtaac tataataagt
     4861 gatttgttcc cctcattttt cattttatgt ttcaagtaat gtttccataa gaggcgagtt
     4921 atatatgtaa ttacaacgta taaacctccc atcaagaaaa gaacgactct agaataatca
     4981 tttccaccct gaacactaat gagatacaaa cttcctaaca gcaccaacat aatcgcttgc
     5041 tttacagaag aagcaaattc tatatagtac cctcttttta atacacctct atatgcttca
     5101 aacatgaaaa taactaaaag atctgccagt tcaatgaaga ctgccatgtt ttgatacatt
     5161 ggaatcacat aaggattcca atctacctgg cgtaagacat acgatagata aaaagcaagc
     5221 tggatacaca ataaatctaa aataataaaa tcaatatgtt taacccatcc ccttgaactt
     5281 ttcttgtaca ttccttttta ctcccctctt tttaataaaa taaattgtat tacttgtttt
     5341 ctctcctaaa tatctttctg aaggattatt gactcgacaa attggcttgt ggatattctt
     5401 atatatagaa catccgtaag ccaacttaaa actccttaac aaaatttttt tactcatatt
     5461 gtagatcaaa acaagtatcc aagaaatcat attttatttc ttttttctgc ttactcaaca
     5521 acagctaaaa gaaaaatcat cataaacaaa ttcaactaag ccaacgatcc ccttgtgaaa
     5581 tcaagttttt tcttgattta cgaataaaga gctaaaataa accaaacttc ttctttttta
     5641 cctcttcata gactggataa ctgatttcat ccccgttgat cagatccttt gcgacttgtt
     5701 tcatctgtac tactttttcc ttaccaaagt cctttgcaat ctgctcatag cactctttta
     5761 agtaaaaggt gcgcttagag accccatggg catcagaagc agccatctgc acgagattat
     5821 gtttgaccat cgttttggct gttttctgga tgtctttgcc aaatacccca acataacttg
     5881 gcgcagtgag ttgggccaaa catcccatat ctaggaacgg aagcaaatga tttgggtctt
     5941 cgcgaaaatg cacgttgcgt tctggatgga cgattactgg tgtctttcct agttctaaca
     6001 actcaaaaaa aagccgttct gcatataacg gcacttccag tgtcggaaat tcgatcagaa
     6061 gatatgtatc atctaaatct gtaaataata tttcatctcg gtggatgtct tcgatgagcg
     6121 ttccggtaat acgtacttct tgtccttcca ataacgtcaa aggcaactgt cttttctcta
     6181 attcggcttg taacgaagcg actagagaaa tgacttgtga tttttcattg ctgtattttc
     6241 cattattgtg gtgtggtgtg cataaaatat gcgtgatgcc ttgctgaatc gctttttcag
     6301 ccattgcaat acttgcttct aagttttctg ccccgtcatc gattcctggt aggatatggc
     6361 aatgtagatc gatcatttct cttgtctcct ctcttcttat tccttaattt ccgtagtaat
     6421 aaccagcatc tttgctatgt tctgctccgt tgtacacaac acctaagact cgtgcttgta
     6481 ccatctccag cagttctttt gctttagtca aggattcttt acgtgacata ttttcacgta
     6541 cgaccaataa agtgccatcc gtttttaaag acatgatctg tgcatctgtt acggctacta
     6601 ctggtggcat atcgaatatc accacgtcaa ataactgtct tgcttcggat aggatctgat
     6661 ccattcttgg cgagcttaac aattctgatg ggttgggtgg cttaggtcca ctaggcaaaa
     6721 tcgacaagtt ctcgactggc gttcgttgga tcacatctgc tacacttcct gatgaactaa
     6781 gtgctgtact taatccactg gcattgttca gctggaatgt cttatagacg acgggtttac
     6841 gtaaatctgc atcgactagt agaactcgtt gacctgattt agcaaaaacg actgcaatgt
     6901 ttgctgcagt ggtggatttc ccttctccag gacctgatga agtcacgaca atcgttttga
     6961 tttgctgacc agctgcggag gcaaactgga tattcgtccg gatcgtacga tattgttcgg
     7021 cgattggtga agatgggttt gttaatgtga tcaaactgac tgcatgagtt tgtgctgctt
     7081 tttgtttctg tgttcgtgcc atttttcttc ctcctagact cttgagcggc ttctgcgtga
     7141 tggggctgtt ttatcgtttg tagcttttgg tcgtgcagga gctgttcctt ttttacggat
     7201 accagcgttc aattctttcg ctgacatttc tggaactgtt cctaagattg ggaatcctaa
     7261 tgtatctgtc acatattttt catctttcac tgtccgatcg agtaattcca ataagaatgc
     7321 cagacctaca cctaccatca ttcctaacat taagccagtc attaaattca atttattatt
     7381 tggcgaaaca ggtgtcgtat tggcaactgc acctgaaatg atcgagattt tatcaacatt
     7441 catcacatct tttgcgttct cttggaaaac ttgtgaagtt gtgttagcga tctgttgagc
     7501 agccactgca ttcgtactta ttgcctgaat cgagaacatt tgtgaatttt ctgcctgatt
     7561 aactgagatg gcatctttga tttgttcagc agtcattttc acattgtctt ctgtttctaa
     7621 acgatctttg acttcattga gcactaaatc actgacgatc atatctttat acgtattgat
     7681 catttgcagg ttcgtattga catcattgac atttgcagtg tctgattgtg gcagtgtcac
     7741 gatcaattgt gcttgtgaac tgtattttgg tgtcaagatg aaaaatgtta cgattcctga
     7801 gatggccaag ccagcaaaca ttgagatgat gattattacc aagtgtttgc gtaatgtttc
     7861 aaaaatttct ttcaagctga ttgtctcttc cattttttct ccactttcct atttattctc
     7921 tagattcatt tgatctttta attctttttt cacacgatca agttcttgat cactgacttt
     7981 ttgataggag atattatctt gcatgaagcc ttctccttgc agctgatctt gcttaatcgt
     8041 accaaatgcc tcccgataat ctaaagcaat ttttttcatt tcatcaaagt tcatgtctgt
     8101 tttcatattc gactctagag cattcaaaac ggattgatag ttgctgacac cgtttagact
     8161 caatactttt ttcgctacgc cttctacgat ctttctttgg cgcccttgtc taccgtaatc
     8221 accatttggg tcttcataac gcatacgaga ataagataat gcctgatccc cgttcaatgt
     8281 aattttaccg attggaaagt catagccatc ttgtgagaaa gtgaggtcat tattgacttc
     8341 gataccacca actgcatcaa cgagttcctt tagtcctttc atattgatag agacatagtg
     8401 atcgattggg atgtctacat atttttgtac ggtatccatc gccatggcag ctccgccgaa
     8461 tgcgtaagcg tggttgatct tgtcttttgt gtcacgacct acgatgtcga catacgtatc
     8521 tcgaggaatg ctgacgatcg ttgtctgttt gtcttcagga ctgactgtgg caaccatcat
     8581 cgtatctgat cttccttgat cgacccgacc taaatctcca gtatcaatcc ccatcaatag
     8641 aacagagaaa gattcttgtt tgtccagatc aactggtttc gttcgttctc cttgggttcg
     8701 ttccactgat tcgtaagttt tctcaataga atgtgagaga tccagatata attttgctcc
     8761 tactcctaca attgctaata ctaatacgat aagaactgct aaaataatca aaaatatttt
     8821 tttccctctt gattttcttc ttcgtcttgc catagtttcc tccgtttgct aaccttgctt
     8881 gaatcaacgt tttaactgtc aatagcttat attgctatag cccatttgct gagcaacagt
     8941 ttgtgctttt gttgcgtatg cggctcttat tttttcattc cggatctgct ctatttgact
     9001 gagcgccgtc aaatattgat cgtaagtagc ggtagctgtt acttgaccgt ctttcagcat
     9061 cgtatcgaat agctgatcta gttgcgtgac ttgtgcaatt tgggtatcag cgaatcctag
     9121 atattgcata acgactgttt tccaagcact atccttaaaa aagctcatat tttcacgtac
     9181 atgggcgaca tctgcacttg ccaacttttc tttgatgatg acatcatcca cagcttgctg
     9241 ccagtttgat acgtttttag taaataattt attgacagcc gtctggatct tcagttttga
     9301 ttctgcatcc tctaactgcg ttaagactgc tttcttttct tcttggatcg cagcagcttc
     9361 tttaggtaat tctgattcct tgatgtcata atcatctgcc gaaagtttga tagaagatag
     9421 tttatgtgac aaatttgaaa ctgtttcttc tgttatttca ggatttagga aaattttttc
     9481 ttttgtactg tacagcttat ctacttcttt tgataaagag gtcacggtgt ctttttgatt
     9541 ggtgatggca gattctgcgt aattgatccg ttgatgatat tgccgctgat tattgagatg
     9601 gtatccaaac aagcaaatca caatagcgcc tgtgcctacc gccacagtag ccaataactt
     9661 gacgaacggc ttttccatag attcactcct aaatattgat gatagataag taaagatgtg
     9721 attcattcac atcagaatga gcgcttttta ttttaaaagc ataattaaac taatatattc
     9781 atagaa
//
LOCUS       DAZKLA010000105         9712 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000105 DAZKLA010000000
VERSION     DAZKLA010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.106"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..646
                     /locus_tag="ACG85N_002221"
     CDS             23..646
                     /locus_tag="ACG85N_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224945.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /gene="trpX"
                     /locus_tag="ACG85N_002222"
     CDS             1096..2145
                     /gene="trpX"
                     /locus_tag="ACG85N_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0224946.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="ACG85N_002223"
     CDS             2142..3023
                     /locus_tag="ACG85N_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0224947.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="ACG85N_002224"
     CDS             3026..3796
                     /locus_tag="ACG85N_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224948.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="ACG85N_002225"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="ACG85N_002225"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFQ0224949.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="ACG85N_002226"
     CDS             5107..6270
                     /locus_tag="ACG85N_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0224950.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="ACG85N_002227"
     CDS             6387..6797
                     /locus_tag="ACG85N_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0224951.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="ACG85N_002228"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="ACG85N_002228"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0224952.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="ACG85N_002229"
     CDS             9370..9654
                     /locus_tag="ACG85N_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224953.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT         3364 a         1648 c         2087 g         2613 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tc
//
LOCUS       DAZKLA010000106         9259 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000106 DAZKLA010000000
VERSION     DAZKLA010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.107"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(176..1942)
                     /locus_tag="ACG85N_002230"
     CDS             complement(176..1942)
                     /locus_tag="ACG85N_002230"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224954.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1902..3656)
                     /locus_tag="ACG85N_002231"
     CDS             complement(1902..3656)
                     /locus_tag="ACG85N_002231"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224955.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3766..4335)
                     /locus_tag="ACG85N_002232"
     CDS             complement(3766..4335)
                     /locus_tag="ACG85N_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFQ0224956.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4353..5768)
                     /locus_tag="ACG85N_002233"
     CDS             complement(4353..5768)
                     /locus_tag="ACG85N_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224957.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(5779..6447)
                     /locus_tag="ACG85N_002234"
     CDS             complement(5779..6447)
                     /locus_tag="ACG85N_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0224958.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6577..7161)
                     /locus_tag="ACG85N_002235"
     CDS             complement(6577..7161)
                     /locus_tag="ACG85N_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0224959.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(7492..7725)
                     /locus_tag="ACG85N_002236"
     CDS             complement(7492..7725)
                     /locus_tag="ACG85N_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0224960.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(7740..8690)
                     /locus_tag="ACG85N_002237"
     CDS             complement(7740..8690)
                     /locus_tag="ACG85N_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFQ0224961.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(8687..>9259)
                     /locus_tag="ACG85N_002238"
     CDS             complement(8687..>9259)
                     /locus_tag="ACG85N_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFQ0224962.1"
                     /translation="KGDTAIGITFSSFLALGVILIGVANSSTDLFHILFGNILAVQDI
                     DKWITIGVSVLVLVIVVLFFKELLITSFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTA
                     MQSVGTILIVAMLITPAATAYLYANSLKTMIFISATVGASSSLLGLFIGYSFNIAAGS
                     SIVLTAAFLFVISFLISPKQKFLRKKERSL"
BASE COUNT         3109 a         1690 c         1215 g         3245 t
ORIGIN      
        1 tttttaaaac tttgattatt ccttatatta taagaataga cattaagtga actaattcaa
       61 tcatactgat tctttcgtgt attcaaaatt agatgctgtt taataaaatt attctttttg
      121 aaaaagaatt ataactatat cccaaaaatt gtaggttaat ttaaagccta tttcatcatc
      181 gtgattgagc ttctttttca tagacgaatt tctcagatag tttagttttc tttatgagat
      241 ttttgtatat ttctgacctt cgatacaact cattatgagt accttgatca actagttttc
      301 cttgatccat cactacaatt ttatttacct tttcaataga cttcaagcga tgcgatataa
      361 ttagtactgt cttatcttgt attaaacgat ttaaactttc ctgaatacgt ttttcatttt
      421 ccgcatctaa actggcagta atttcatcta aaattaaaat tggcgcattt ttcaaaaaag
      481 cccgtgctat ggataggcgt tgtcgctctc ctccagaaag tgaacttccg ttttctccga
      541 ccaatgtatg ataaccgtca ggtagttttt caataaagtc ttcgcagtta gctaaacgag
      601 ctgctttttt cacttcctca tctgacgctt gactatttcc aatacgtata ttttccaaaa
      661 tacttgtatt aaacaaagtt acctcttgaa aaacaatagc gattttgcta aacaaagaag
      721 ctggagatac acgtttaata tcatacccat caattaaaat acagccttca tcataatcat
      781 acagtctaga cactaatttc aagatactag ttttcccgga accactagca cctactaacg
      841 cagtcacttc tccttgttta gccgtaaaag agatatgatc taaaattggg gtattgttat
      901 catatgagaa agagacatct cgcaactcaa tgtcaaaaga tttcagagga ctatctgatc
      961 cctcttggat actggtttcc ttcatagctc taattcgctg tattttagga gctaaataaa
     1021 atatttcaag cactgcttct ttcatagaat cgaaggaatc ctttattttt attgcggcta
     1081 gtaagtaacc aacgacgtag agtatagtca cttcacccgt taacagtaag tgaacaccta
     1141 ctagaatgac tactgctaaa ctaacatatg agaaaataga ggataaggcc atgactgaaa
     1201 aagtacttaa ttctaccttt aaatgaatcc tttcactctc ttccattttt ttgaaaagtc
     1261 tgtcttgaac ttttttagat agattaaagc tattaatctc ttgctgcaat tctatagttt
     1321 cttgaaattc ttccgagttt tctctcaaag tatcgtaata tttagtattg gctttcgttt
     1381 gggatttctt tgataacaag attaagacaa aactaaataa cgttggcaaa ataacagcta
     1441 atcccatttt gacattacca accaaaagca tcactgaaat aaaagggaaa aacagagcca
     1501 taccgccggc tttaggtatt gcatgactca tcgcatgctc aataccttca acgtcagaca
     1561 tgatagtttg agataaatct gataaattat gttttgaaaa ataggataat ggtaaatttg
     1621 acaaatcgtc tgcaatttgc actcttaaat gcgcactttc tttataagta ctattatata
     1681 agttttcata ctccctatct aacaaccaat acatgaaaag caaagtaaca aacgatacgg
     1741 caaaataaag ccaacggggc tttgtatttt ctaaaactaa ttcgtctatc agcatcatta
     1801 taatgaatgc tggtaccata ttaatgcagt atacaaaaaa actagaaata gaagctttag
     1861 ttaaagcttc tgcgccctga tcacttaaag caaatttgtt tttataaaaa cttttcattg
     1921 tcaagcctcc aatcatttgc attctcataa ttagtgacca attgtttata cacttgatgt
     1981 ttagccatta attcattatg atttccacgt tctactattt tcccttctga caagacaaga
     2041 atttcatcta cagatttaat gctggataat cgatgagcta tcataataac tgttttttct
     2101 ttcatcagat gttgaaaagc cttttgtagt tcatactcat tatctggatc aatagatgca
     2161 ctggcttcat ccataataac aatctttgca tcttttaaaa ttgctctggc aatagctata
     2221 cgttgttttt ctcctcctga cagataaact cctttactac caattaaggt atgctctctt
     2281 tcgggaaatt tttctaaaat cgattcgcaa ccggctaatc ttagcgcgtc aaatacttta
     2341 cttgctggcg cttgaggatt tgctatagaa acgttatcgt aaatactctg attgaatagt
     2401 ttcgtatctt gaaagacaaa agcaattgtg ttaataattg tgtcttttga atagtgattt
     2461 aaaggcttac caccaatttt aatctgtccc ttatccacat tgtagaaacc tgaaattagt
     2521 ttagctatcg tactctttcc gcttcctgat tttcctacta atgcataaat tcttttttgt
     2581 tctaaaacta agttgaaatt ttctaaaaca cactcttctt tgtatgaaaa gctgacattt
     2641 tcaaatgcaa tgtcacaatt tttaatctgt gtctcgttcc catattttag ttggtcttgc
     2701 ttcatttcta aataaaggga gtctagtgtt tcgactgcat atcttgcttc aaacaagtgc
     2761 atacttacat acattattct catgaaagaa acaaaaataa ttccacttaa aaaaaacaac
     2821 atcaacaagt caacagctaa tatcttggga gtggtaagag aagttataaa aaaggttata
     2881 ggaactatca aaatgcaaac tatgccaaaa aatagccatt gaaaaagaac aaaaggtgtt
     2941 ttcccactca aagaatactt atatgcaaac ttagcataat cgttaattgc tttattcaag
     3001 gctttaaatg atgttacttt agcaccaaat atctttataa cttgaattcc gcggatatat
     3061 tctaccgttt cagagcttaa cacatctaac gattcttgat atttttgaat aaaatttgta
     3121 ttctctgtca ttttctttaa caagaataca ccaattacta acatggcgat tagaaccaat
     3181 ccaattttaa agttaatgaa aaagcctaag gcaactgata aaacaggtgt tactatggct
     3241 tgagcactat ctggaatcaa gtgagcaatt gccatatgtg ttttagccgc gttgtcatca
     3301 attgtctttc gaatatatcc cgaagaattt aaatcaaaaa aacgaaaact tgcttctgtt
     3361 aaaccgctaa ttcccttttt tctcaaatta gtctctaagc gaaacccaag gatatgagaa
     3421 aataagccag aaaaaatata aaataaagct cctaaagtta accaagcaac agtttgaacc
     3481 gcataactct tagctaaata ttcatctcct acaataatta gagactgaag aaatttgtaa
     3541 atactgtaat atccatagac tgttaatacg acagaaaaat cagaaaataa agtggctaac
     3601 catccgtagt atttcatttt agggacataa ctaaataatt ttttgtatac tttcattttt
     3661 tcctcccccg cattcaattt cttctcttat tcgtttacaa ctgttaatgc tttccgtaat
     3721 agcgtaaatt agagatgtac aaacagataa aaggtaaact acgttctatt ttttgtattt
     3781 ttttaagact tttcttccaa tcaatacacc aatcatagat aaacttgcta caactaacac
     3841 tgaaatgatt ccccatatac ctaccgcata gtggatcatt gcatccattg ttgcagcaga
     3901 ttgtccttta tctatccaat cttgacgaaa acgatctgta aagaaccata taggaatcca
     3961 ctgtccggca gcactgccta attgcattag agcccaagac gcaacttgag gaattgctcg
     4021 atcatatcca aatacagata gaataatatc agctaagatc gccatactaa tcgaactgat
     4081 aaaccaaggt aagtacgccc cgcccattaa cgtaaaaaat aacaataaaa caattgtgta
     4141 aatagtaaaa attccttttc tagggtagtt atagctaata taaacaaata tgattccaga
     4201 taataatcca aaaatcccgg gggatatgct gtgaccaaac gccccaccaa tactactaac
     4261 cattattgaa aaaaagtaaa ttactaggga aataactgta gctaaaccaa taaaaatata
     4321 atcttttgtt ttcatatata ttctcctcaa ttctatttcg ttatgtcatt aaaaatctgt
     4381 tctatcaaag ttgaatcttc tactctaagt tttatatctt tttttattcc accatttttt
     4441 aaataaataa ttctattgca tgttttataa agaaattccg gatcatgagt agctataact
     4501 actgcaccct tttcttttaa gtctttaata aggtcagcaa tcctttccat attaatataa
     4561 tccaacccac ttgtaggttc atcaaataga aataaattag tttttgacaa tgaagctata
     4621 gaaaccaata atctttgttt ttctccaccg gataaatcaa acggtaattg atctttcatt
     4681 ttataaagtt ccattttttt taaaattttt tcttgctgat ctttactaac tatttcattc
     4741 cccagggcta actcgctcct cactgattct gtgaaaaatt ggtaatccat ttcttgcatt
     4801 actaaaaacg gcgatgacaa atttttttta ttttcgatta tctttccttt ggtagggcga
     4861 ataatcttca tgagtattct tagtaaggtt gttttcccca caccgttttt ccctaccaaa
     4921 ccaataacat ctccaacttt gacatcaaaa tttatgtctt ttagaatatt ttcataagta
     4981 atttcttcaa cttttaatac atctgcactt accttttcga tatagggaac ttcaatcgaa
     5041 ctattaaaaa tgtcaaattt ccttaatgcg ttattgtttt cagtttttaa caactgaata
     5101 gttgtagttt ttacagttct atttttaaca aatagaactt gatcaattaa ttctttcaaa
     5161 tagtaaaaac gatgttcagc tataacaata gtaaatcctt tattttttaa atcacaaagt
     5221 aagtcagcaa gtttcttagt cgctttgaag tccaaatttg aagacggttc atccaatata
     5281 attaatttag agtttagcga taaactacat ccaatagaaa ttttctgccg ttcaccactt
     5341 gatagcgaaa aaatgttttt atctaatata tttgaaagat ctaataattc agctaattca
     5401 ttgattcttt tatccatatg ctcttttgta aatccaaagt tttccatcga aaatactaac
     5461 tcactcgttg tatttgttgt aaagaattgt cctctaggat cttgacttac gtagccaata
     5521 ttttttgata tactttcttg tgttaaggat tgtagcgagt tattttcaaa taaaatttcc
     5581 ccaaccaatt ttccgtcata aagtcttgga ataagtccat tcattaactt taatagtgtg
     5641 gatttcccac tcccagaaga accagtaatc aatgtaagac tgcctttttt tattgaaaaa
     5701 ttaaggtcaa caagcgcttg ttccttaaca tccttatatg aaaaagaaac attgttaaat
     5761 tcaaacaatt ctttcacctc aaatcaaaat acttaagaaa aaaatagtaa cggaaaacag
     5821 gatgatatag tctaataagc tcatgtttgt tttatagtaa gaacttcttt tcacaggaga
     5881 ttctatcccc tttgttattg cattagcggt catttcttca gcaatcatag ttgcacgtat
     5941 aagaacggga actaacagaa attccaaata ttgcagaggc tgaaaaaaag acaagcgaat
     6001 gtttctcatt tttaaagatt ctttaatcat tcttatttca gaacgataag tcggaaagaa
     6061 tctgatcact actgtaatac ttaacattat ttttttcggt aaatgtattt tttctaaaga
     6121 ggataataat tcattgctag tatagacact aaccaacaac catgagatca ttacgaccgg
     6181 caacattcga aatacaatat ataaggatgt cgcaaaaaat atagtaaaaa actctatctt
     6241 gatagcatca ataattttca ttactaaaaa cagaactgaa taaatcaaga tgaaataaat
     6301 acatgtcttt attttacgaa acatcaatac taaaactgta gcaataatga aacaccccac
     6361 ttgaaatata tttccctttc ctaaaaaaat aatgaccgga aaaaaaatat ttaaagtaat
     6421 caaggttaac ggattgattt ttgacactct ttcacctcca aaaaataaga tcgcactgtt
     6481 atcttaagct gataaaaatg caccgttatt cagataacag tgcgatctta tcaaaaagca
     6541 taactgagaa caattctcaa tgcaagcttt ttttaattaa ctcagtaatt caatccaccc
     6601 aggaacagaa aattgataca atcttctggt taatagcata gtcttttctt ttgaatagct
     6661 atgcttcaat atatcataga atgtagcgta gaatgagtaa atgtagaagt ggatttcttc
     6721 ttcattaaag actaaatttt gattgatcat tccgatagtc tttaacttat ctagacaaca
     6781 gactgtcttt ttcgtcacaa atgatgtaat tttttcaaca atatcttgat atttggttcc
     6841 agctgattgg aataaaatca aatcgaatat acgtttattc tcaaaaatat aattaataaa
     6901 tggatctaat tcattgaagt tctctctcca acttttctct atatctatat tttctaagtt
     6961 taagaggtat ttggtaaact cttcattata catattaata agcgcattaa tatgcggctg
     7021 aataatttct tcaaataacg cttccttaga agtaaaatgt ttataaaaag cacccgtagt
     7081 tacgcctgcg gtcaggcaca gttgccttat cttagtttgc tgaaatcctt ctttttcaaa
     7141 atggcttagt ccactttcta ttaacttttg gtgagttata atataatctc tggtttctat
     7201 gggtacttcc tcctaataag atgaattaac tttattatct tacacaggac ttgaagtttc
     7261 aatcgttaac gtttattact gaactcattc cttagagcta aactaatgag aaagagcaga
     7321 ataactgata caaaaagcaa tcctacaaga attaatagac tcatctaaat cattcacctt
     7381 ttctaaaata atccgagaaa gatttaaaaa aattatatcc agctattagg ataagtccta
     7441 tcataatcac tccaatagcc attttcttat cctccctaaa aataaagtct tttacttaaa
     7501 accttttttg taatcgtcaa taaatttttt ccctttatca gttaattgaa atcctatatc
     7561 taaatagtcc tcatatctaa gtttagccag catctcactt acagatgctg gactaacact
     7621 tagaactttt gctaaatcat tattttttaa tttctttttt ctttttgata attcctgaat
     7681 tgctagtaaa taaattagta tagtagtcgt tttaatttgt accataccta ccctccaaat
     7741 tatttcgaaa ggccttcagc aattttatcc aggttccatt tcatcatcgc atagtaacta
     7801 tcaccatctt gtcctttttc tgcaattgaa tcagtaaaaa tcgttgaata gataggaata
     7861 ttggtatctt ttgatactgt tttcattggt ctatcatcca cactgctttc tacgaataag
     7921 gagggaactt ttgttgtgcg taatttttca actaagtgtt ttatttgatc tggtgttcct
     7981 tcttcttcag tgttgatttc ccaaatgtaa gcagaaggca cattatacgc tttcgagaaa
     8041 tatttaaagc atccttcact tgtaacaatc atttttttat cattcggaat tgaagcaaat
     8101 ttagatttag cttctttgtc tagagaatcc aatttttcaa tatacttatc tagattttct
     8161 ttatagaatt ttttattatc aggatctttt tccgctaact gtttttcaat atttttagcg
     8221 taaataatac cgttttctaa atttaaccaa gcatgaggat cttccttccc tttctcactc
     8281 tgaccctcta agtaaataac atctatgcca tcacttgctg caaaatagtc tttgttttcc
     8341 tctttgttcg catttttaac taattttgta aaccaagcat ttcctccagt ttccaagtta
     8401 acaccgttat aaaaaatcaa atctgctttt gaagtttttt gaacatcttc aggcaaaggt
     8461 tcatattcgt ggggatcttt cccaataggt acgatactgt gtaaatcaat tttatctttt
     8521 gctatatttt cggtaatatc tgctaaaatc gaattagtaa ctactacttt taatttttcg
     8581 ttcgaagcca ctttatcttt gtctttacta tttattgaac atgccccgaa tataaaaaga
     8641 gcaatcaaaa tccccagact tcccagtact gctttccatt ttctcatcat aacgatctct
     8701 ccttctttct taaaaatttt tgttttggtg agattagaaa actaattaca aataagaatg
     8761 ccgctgttaa aacaatactt gatcctgctg ctatgttaaa actataacca ataaacaatc
     8821 caagcaatga tgaacttgct ccgactgttg cagaaataaa tatcatagtt ttcaagctat
     8881 ttgcatataa atatgctgta gctgctggtg taattaacat tgcaacaatt aatatagttc
     8941 ccacactttg catagctgtt actgctacta aagttaatag aaccatcaat agataatgat
     9001 aaaaactgac attcatccct attgcttttg ccattaatgg atcaaaagaa gtgattaata
     9061 attctttaaa aaatagaact actataacca acactaaaac cgaaacgcca attgtaatcc
     9121 acttatcaat atcttgtaca gctaaaatat ttccaaataa aatatgaaat aaatccgttg
     9181 agctatttgc aactcctata aggataactc ctagtgctaa aaaagaactg aaagtaatac
     9241 caattgctgt atctccttt
//
LOCUS       DAZKLA010000107         9140 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000107 DAZKLA010000000
VERSION     DAZKLA010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.108"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..1444)
                     /gene="ltrA"
                     /locus_tag="ACG85N_002239"
     CDS             complement(56..1444)
                     /gene="ltrA"
                     /locus_tag="ACG85N_002239"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0224963.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(1897..2649)
                     /locus_tag="ACG85N_002240"
     CDS             complement(1897..2649)
                     /locus_tag="ACG85N_002240"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0224964.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(3013..5679)
                     /locus_tag="ACG85N_002241"
     CDS             complement(3013..5679)
                     /locus_tag="ACG85N_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0224965.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6020..7471
                     /gene="cls"
                     /locus_tag="ACG85N_002242"
     CDS             6020..7471
                     /gene="cls"
                     /locus_tag="ACG85N_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0224966.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7800..8615)
                     /gene="thiD"
                     /locus_tag="ACG85N_002243"
     CDS             complement(7800..8615)
                     /gene="thiD"
                     /locus_tag="ACG85N_002243"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFQ0224967.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            8909..>9140
                     /locus_tag="ACG85N_002244"
     CDS             8909..>9140
                     /locus_tag="ACG85N_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224968.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEK"
BASE COUNT         2546 a         1970 c         1592 g         3032 t
ORIGIN      
        1 aactgacctt ccgcccctct cacaccaccg tacgtacgga cccgtatacg gcggttcaat
       61 aagttgagtg tacgaactgg tacttcaagg agatgtcctc atatccttgg cgggccagtt
      121 ctttgtctgt taatgatcga tgaagaatat gacttttgga gactgaccaa ataccttttc
      181 ttgtatttga ccattcatac gctttcgctt tatcgatccc taaacgaatc agattttttc
      241 ttttggttct tggattcttc cattgtttcc aaatatattg tctgattctt cgcttcaacc
      301 aaccgtttaa ctccctcatg aattctttca tctcaccaat tccatagtag ttgatccaac
      361 cagtcattag ttgcttgatt tcttttaaaa taagttggat acttcttcca cggtttcttt
      421 tagtgagttg tttgagcttg cttttcacac gctgttttgc ttttgcgtga gggcgaattt
      481 taataccttt ctttgttggc agaatgcaga atccaagaaa ctttcgtttc aatggacttc
      541 ctaccgcact cttttctcga tttaccgtca actttagttg ttcttcgaga aattgaatgg
      601 atttcttcat cacacgctgt cctgctcgtt tgcttctaac atagatattg cagtcatcgg
      661 cgtatcgaac aaatttatgt cctagatttt ctagcacttg atcaaattcg ttcaggtaga
      721 tgttacttaa caggggagag agattgcctc cttggggcgt tccttcttca gttgttgtga
      781 tgagtccatt ttccattaca ccactcatta agtattgacg gatcagtttt aggacttgtt
      841 tgtcctttac tttccgctca atgaaataca tcagcttatc gtgatttacg gtgtcaaagt
      901 aggctttcat atcaatgtcc accacatatc ggtaaccttg ttcataataa gattttgctt
      961 tcttcactgc gtcatgtgcg ctgcgatttg ggcgaaaccc ataactatta tctgagaatt
     1021 ctggttcaaa tagcggttgt aacacttgat tgattgcctg ttgaagcata cgatcaataa
     1081 ccgtgggaat tcctagtttt cttacaccgc cgtttggttt cggtatttct actcgcaaaa
     1141 caggctgagg tttatagttc ccttggcgta aattggatag taattcctcg cggtgctctt
     1201 tcaagtaagg aagcagttga tcgacggtca tcccatctgc accagccgcc cctttatttc
     1261 tgactacttg aagatacgct tgattgagat tattccgatc tagaattctc ccaaacagat
     1321 tttctacacc atcttgtttc ctctgttcac cgcaagaagt gctgcgcact tcatcatacc
     1381 cttcgtgttc cacactagct ctttgatgat agccaagttt tcttgttttc tgcggtcgtc
     1441 gcatcttctc acacctccta aattattcaa gattcttatt gttcagtcct tcgtaacggt
     1501 cagtgtcgtt actactatga cctcggctga cttctcatga ttcaccatca tgtcgccatg
     1561 atcgttgtgc tggtttgttt tcattcaacg cccacaacac cctcatgaga tctcccccgg
     1621 taagagtgaa aactttcctc ccatgtaact gctagattta ctgtataggt ttcgggcagt
     1681 attggacttt gtcttgtgat gaagacttat ccgacctaat tcagccttgt atctagtttc
     1741 tgttcgtcag ttcaggagtt tgcgtccgac ttccttcagc cacaacctca cgattgccgc
     1801 cttgtctttc gctaacagtt cctactgcca agcctgtagt ggactttcac caccaagttt
     1861 tcacccatgc agggcgcaca tcataaaaag aacccttcac gagtcgtgaa aggttctttt
     1921 tttatctgcg gaccaaagga gcagtctata ggtcagctta gtcgtactaa ggatttctcg
     1981 tttttcactg aatagtttgc gaatgtacct ggtcacaaca ggatcttttg cgtacgcgtc
     2041 atagtatagc ttcatcaatt cttcagtaat tgattcctgt gtaaaataag taaaatcttt
     2101 tttcttcact ttgaatcctt catcaattaa gtatgtttca aattgatcca ttacaactgg
     2161 cgatgtatta ggatcttctt ctatcattcc tagttgcttt tcgataggat aaaaaacatc
     2221 atcgattgat tccactactc tgattatcac gcaccatttg cttgactttt tcatcaattt
     2281 ttctaattga ataagatgta ccaatgctgg atttgctgct gtaaaaacca tttcatatgt
     2341 ctcatttgat tggataaatt cttctaacga ctgtttgata aaggaaacat ttggcaactc
     2401 atttttttct gcttcttttt ttgcattttc tagcattcct gaagaaaaat cgatcaaatc
     2461 atagcgatta atttcttttg ctaaggaaag aaatctgcca gatcctccag caacgtctgc
     2521 tgctgtttca gcaggcagga ctttctccgt tttcagataa ttttttacat cttcagcaat
     2581 agttacttgc gattcttgct ggatttcata atattcttca gcaaattcat cccattcttt
     2641 ttcattcatt tgatttcctc taattttttg ttatatgttt tcacttttcg gaataaatca
     2701 ttctcctctt ttatctattc tatctctttt gatgaataat aaaatgattg gctttccgtc
     2761 tatcataaat aaaaaaaggg actgtgacca aagtcgtgtc aaagtccctc tttccgaata
     2821 aacagtgtcc aatcagatga ccctcagtat tagctcaaaa tgctgatttt acaacttctt
     2881 ccttaaacgg tgttctcctg ccaactcttc ggtcacaagt cacaatattc aacaaacatg
     2941 agaagctgtt tgttgaaatt gcaccttgtg actcagaatt aaacggcagg ctcacaacct
     3001 cgttgattcg ttttatttat ttccttgtac tcgtacaatg atcaatttag tgatttccat
     3061 caaaacaatt gtcacaagtg cgattcccat acatgtaaag acttctgtca atccaaatgc
     3121 tgatgggata gagaagactt ctttcagtcc tgggatcatt gttaagcagt aaagcgcgct
     3181 acatacaact acagctagta agacacttct gttagagaag aaccctgctt ggatagctgt
     3241 ttgtgtgttt gaacgtgctg ggaatgtttg caatgtccga caccaaatca atgttgaaaa
     3301 tgacatggct acaccgaatt caggtgaatg ctgcatgcca agccattgag caaagataac
     3361 agcaatccca atcagcacac cacgatacgt tacagaaatc aatgttttgt ccgtaaagat
     3421 tcctgaattt ggatctcttg gcgggcggtt catcgtagca ggttcagctt tttctgtacc
     3481 taatgcaatt gctggcagtg aatcattgac taaattgatg aacaacagtt gtaatgctgt
     3541 aaatggattg gaccagtcag caactaaggc aaagatgatc gcaatgatcg cacctaaatt
     3601 tcctgcaaat agataagaaa tagctttctt gatattatca aacacattac gtcctacttc
     3661 tactgcgctg acaatagaag caaagttatc atccgtcaat accatcgctg ctgcatcttt
     3721 tgccacatct gtacccgttc ccatagcaat tccgatgtct gcttgtttca atgctggtgc
     3781 gtcatttaca ccatcacctg tcatagctga gatatgacct ttattttgcc aagcacgaac
     3841 gatacgaatt ttattctcag gtgaaacacg tgcataaaca gtgacttttt ctaaaacttc
     3901 gtttagttga tcttctgtca attcatctag ttccgtcccg gtcaaagcca ggtttccttc
     3961 ttcaaaaatc ccgatttgtt ttgcgatagc aacagctgtt gttttgtggt caccagtaat
     4021 catgattgtt ttgattcctg cacttcttgc ttctgttacc gcctgagtca cttcagcacg
     4081 tggaggatcg atcatagcaa gcaaaccaac aaggatcaaa tcgtcttcat cttcaaatga
     4141 taaatcaaaa ttatcaagtg gacggtaagc aaaagctagt acacgtaatg cgcgtgttga
     4201 aaaagcttca ttttgctctt ggaatttttt catcaattca tccgtcatcg gaacgacttc
     4261 accatttagc aatacttttt tactgcgttg gaagacgata tctggtccgc ctttaacaaa
     4321 caagtaattt ttgttatcaa ttgtgtgtcc agtagacatt aattttcgat ctgagtcaaa
     4381 aggaagttct gcttgtcttg ggtagttgtt tcggatttcc tgataaggct ggtttaattt
     4441 ttcactaaag tcgataaacg ctacttccgt agggtcgccc aatttagtac cgtcttcact
     4501 gatcgatgca tcattcgcta aaacactgat ttcgatcaag cgacgttcgt cagctgtcca
     4561 ttttgaaggg tcatttgtga aatccccagt atcgccatta cctaaaaagt agtcaacaac
     4621 tgtcattttg ttttgtgtca atgtaccagt cttatctgta cagatgatac ttgtcgaacc
     4681 aagtgtttca actgctggaa gcttccgaat aatggcatga cgttttgcca ttttattcgt
     4741 tcccaaagat aacacgattg ttacgataga ctgtaatgct tctggaatag cagctacggc
     4801 tacggctacg gcaaacatca ctgcgctgac gatatctgca gtcatatctc ctgtaccttc
     4861 tccaagataa atacgaaaca gttggatacc taaaatcaat aaagacaatg ccaaaatagc
     4921 caaacttagt tttttactga atttatccag acctcgttgt aacggagtga cttgctcagt
     4981 tgttgattcc aaaaggccag caacttgtcc gatctccgta ttattcccag ttgaagtcac
     5041 taagaattta ccgcgtccgt aagttacgat cgttccactg aataccatat ttgtacgatc
     5101 tccgattggt accgtacttg tgatatccgt tacttctttt tctgcagaag tcgactctcc
     5161 tgtcagcata ccttcatcta cttttaatga tcccgcatct agtaaacgtc catcagcagg
     5221 tacataatcg cctgcgtcta acaacacgat atctcctacc acaagttcgt ttgccggaat
     5281 cgttttttcc ttaccatcac gaatcactcg agcatcgggc gctgaaaggt ttttcaacgc
     5341 ttctaaagaa ccttctgctt tttttgtttg gacaacactg attatagaat tcaacatcaa
     5401 tactgcaaaa ataactaaga attcgatgta gtcacccatg accatctgaa tcagtgcgac
     5461 aatcagcaat acgataacca ttgcatcttt gaaagtttct aagaacaatt tccaagttgg
     5521 cttcttcggc ttttctttta gctggttcaa gccatctctt tctaattttt ccttcgcttg
     5581 cgtggtgctc aatccatcgg ttgagctctg cgtttgctgc atcaacgagt cgatcgtttc
     5641 tttatagacc gcttgttgtg cttgttcctt gttttccaaa tttcatcccc cattttttga
     5701 tcttggccct agtcctgtct gaaaaaaaag agacctaaga caaatacgca tttgtcttag
     5761 gtctcaccat ttcatacaat accagaaaac catttggtta acttactgtt gatattatcg
     5821 caacaaacgt tgccggttac tcccctaagg agatattcaa ctgtactaat atcctacctt
     5881 attaacctac aaaaagcaac tactttagcc cgatttacct acattttcat gaaaggagtg
     5941 aaaagtatat tcaccttggt aggcttcttt cttctatcat gctatactaa tgttattgta
     6001 tgtaaggagg aacatttttg tggtatctag tattataacc gccctttatc tattaaatgc
     6061 acttattgct ttggtggcta ttttgatcaa accccgagat gtagcagcca tttgggcatg
     6121 gctcttagta ttgtttgccc ttcctggggt gggctttgta ttatatttat ttttcggacg
     6181 tggattaacg gataagaaaa aattctatct ccgacaaagt gacttgaaag aattagaaaa
     6241 ctttcagtct tttagaggag ataccattga acattacgat cctgacatgg gcgataaaga
     6301 caaacagcaa tttgttgact tcttctcttc attaaatcgt atgccgctga caagaatgaa
     6361 ttctgtcact cttctcacag acggacaaga gaaattggat tcactgcttc aagatctaaa
     6421 aaaagccaaa cattcgatcc atatcgaata ttacgcattt gtgacagata atatcggcca
     6481 gcaagtctta catgttttag aagaaaaagc cgcagaaggc gtggaagttc gaatattata
     6541 tgatgcattt ggctctcatg gcacaaaagc aaaagatttc aatcgtctaa tcaaaaatgg
     6601 tggacatgtc catacatttg ttacctcaca aagggcatta cttcgtttcc gattgaatta
     6661 ccatgatcac cgaaaaatcg ttgtgatcga tggaaagatt agttataccg gtggtttcaa
     6721 tattgccaat caatatgtaa atacaacaaa aaaattcggc tattggcgcg atacgcatat
     6781 acggattttc ggtgccgctt cttctttgct ccagcttcgc ttcttaacag actggaacgt
     6841 ctcggtacct gaagaaaaaa aggtcggcta tcatttgaat tatttcttta aaaaagcaga
     6901 tcgagatgaa tctaagcttg ctgatacatc catccagctt gtttcaagcg gaccgaataa
     6961 cgaaagggaa caaatcaagc tttcatttat caaattgatt acttctgcta aaaaacgtgt
     7021 ttggatacag acaccttacc ttgttcctga tgaaagtgtc attgctgctt taaaaatcgc
     7081 aactgcctct ggtgtagatg tgaaaatcat gattcccaac aaaccggatc atccttttat
     7141 ttatcgagca acacaatatt atgctcggca gctgatcaag gaaaatgtac aaatccttgt
     7201 ctatgagaac ggcttcctcc atgcaaaaac attgataatg gatgatgaaa tctgcatggt
     7261 aggttcagca aatcaagata ttcgtagcta ccgattgaat tttgaaacaa gtgctgtcat
     7321 ttacgatcct gagtttttag aagaacttgc tactcagttc aaagaagatg agacacattg
     7381 ttcatccatg acaactgaaa cagtcaagga aatgtctaac tggctattat tcaagcaaca
     7441 aatttctcga ttattttctc caatcctata atttttttat tcaagtagag aaaaatatac
     7501 taagattaaa gggactgtga caaaaatctt gtcacagtcc cttcttccga acaaacggtg
     7561 ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgtttt
     7621 ttgaacttgt tccttgtgac tcggagctgg acggcaggct cacgacctct ttcttaaacg
     7681 gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca taagaagctg
     7741 tttttacttg gctgttctta ttacttgatg cagggcaact ttttcacaac ttctttcttt
     7801 tactctaatt caccagctgc ccaatgattg atggggccat atttgtgccc tacatttatt
     7861 tcttcacgaa ttgcacggtt tacatagtct tttgccaatc ggatcgcttg ttcaactgtt
     7921 tttcctttcg ctaactcagc tgcaatacat gcagatagcg aatcgccagt tccgttgatc
     7981 cggtcggtag gataaaatgg tgcatttaac caaaatgatt ttcctgattc gaacaatacg
     8041 tagtcattga ctgtttcttg ctgcgcattt ttagcatgat gtcctttgat cataacgttt
     8101 tttgcaccca tatcttgcag ttcttttgct gcttgcacaa aatcttcttc agctgctaat
     8161 gtccgtccag tcagcttttc tgcttcataa aaattaggtg tcagtacttc ggccaaaggg
     8221 acaagctttt ctttcaagct ttgcaaagca gattcttcta gtaaaagatt tccatgtttc
     8281 gttacgatta cgggatccac gactaatggt ccaaaatcat atttttggta attttttaca
     8341 actgtctcaa tcaactttga atctgctagc atacctgttt tagctgcacg tatttgaaag
     8401 tccttcgcaa tcagttcaaa ttctttgtcg ataaaatctg ttggcaatgt cacacttgct
     8461 ccgattccgt aagaattacc tgcaacacat gcggtcatga cagaaacacc gtaaacattt
     8521 cttgtaaaga atgtatgcat atctgcttgc atccctgcgc ttccatcgct atctgatcct
     8581 gcgatcgtca ttgcttgtac atactgatct tccatcaaat cgattcctcc atttttataa
     8641 aaagattttt cataatactg ttactgtttc tatcatatgt ttttcaaagt atccctcagt
     8701 aaatatacca ctaccttttc ttggtaatca aatttatttg aaaaaacatt tgcaataaat
     8761 cagaaaatag tggtcgttta tttgtattcg ctattctaat aataaataac cactatttga
     8821 tttatatata tttcagtatc cttcttgttt tcatataacc aaaggcgcta tgatttccat
     8881 atgaaaaaaa taaagtcagg tgaaaaaaat gaaacttgag gataaaaaag tcattttgaa
     8941 gaaaatcaaa cgactaccag accaaataga tggctatacg atctgttctt tcaaaaaaga
     9001 caggagtata aaagcttata cgcaaaacac agaaaaagga ataatctatt tcctacatga
     9061 agaaggttac gatacccaaa cctttatgtt tactgaaaaa aaagaatttc ataaacagat
     9121 gaaaaaactg attgaaaaag
//
LOCUS       DAZKLA010000108         9118 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000108 DAZKLA010000000
VERSION     DAZKLA010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.109"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(67..891)
                     /locus_tag="ACG85N_002245"
     CDS             complement(67..891)
                     /locus_tag="ACG85N_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFQ0224969.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(864..1544)
                     /locus_tag="ACG85N_002246"
     CDS             complement(864..1544)
                     /locus_tag="ACG85N_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0224970.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1567..2505)
                     /locus_tag="ACG85N_002247"
     CDS             complement(1567..2505)
                     /locus_tag="ACG85N_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0224971.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2750..3601)
                     /gene="ispE"
                     /locus_tag="ACG85N_002248"
     CDS             complement(2750..3601)
                     /gene="ispE"
                     /locus_tag="ACG85N_002248"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFQ0224972.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3755..6328)
                     /gene="mprF"
                     /locus_tag="ACG85N_002249"
     CDS             complement(3755..6328)
                     /gene="mprF"
                     /locus_tag="ACG85N_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0224973.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6469..7275
                     /gene="proC"
                     /locus_tag="ACG85N_002250"
     CDS             6469..7275
                     /gene="proC"
                     /locus_tag="ACG85N_002250"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFQ0224974.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7354..8910)
                     /gene="rny"
                     /locus_tag="ACG85N_002251"
     CDS             complement(7354..8910)
                     /gene="rny"
                     /locus_tag="ACG85N_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFQ0224975.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT         2690 a         2014 c         1567 g         2847 t
ORIGIN      
        1 tttattaaaa taaaaagaag cctataaaaa atttttcgtt tttataaggc ttcttttctt
       61 tataatttag ttattttttc ttctgaaggc aaccaatatt cgtttgatga tatttacaat
      121 caggaacaaa gcaataaaga ctaatgtgat tgttgctcct ggaggtgtat ctagataaaa
      181 cgagctagtt agtcctgaca gcatccctag aaatcctaca caaacactga tagcaatcac
      241 gacattgaag cttttgccta atcgcatacc gattgctgcg ggaagaacca tgattgcgga
      301 tatcaaaagt gcaccggcaa tcgggatcat cacagcaata gccacaccag taatcacatt
      361 gaacatcatc gacatcaaat gaacgggtag cccatccacg tgggcggtat cttcatcaaa
      421 agtcagaaca tacatcggcc gtttgaataa aaagaacaat ataccgatgg caacgaataa
      481 gattcctaaa aagatgactt gctcccatgt aatggtcacg atcgaaccaa acagatagga
      541 ttggatactt gttgctgaat tccctccgct caaattcatc aatactaacg ctagcgccaa
      601 accaccagcc attaaaatgg caatggagat ttctgaatag gttcggtaga tcgttcgcag
      661 atactccaat atcacagctg ctaatacaac gactagcatg gtcgtaagtg taggattaag
      721 gttcaaaaag aatcctaacg ctacccctgc taaagagaca tgagaaagcg tatcagccat
      781 cagcgattgt cttctgatca ctaaaaacac acctagcatc ggggcgattc cggcaatgaa
      841 gatagcggca agaaacgctc ttctcatgaa ttcatatgaa agcaacgcca ttgagaatcc
      901 tcctttctca ccagtcggat ctgtctgtcc gcatactgtt tgatatcttc atgatcatgg
      961 gtgatcatta gaactgattt cccatggaca tgcgcactat gtcggagaag acgataaaat
     1021 tcatttctgg attcttcatc catacctgtt gttggttcat caagaacaaa taagtcaggg
     1081 tcagtagcaa aaatacgcgc taaactgatt cgctgctttt gtccgccaga caattcacct
     1141 attcgccgat ttcgcatatc ccacatccct acagcttcta aagcacgctg tacatgttga
     1201 tgatcttttt cagagagacg cttgaaccag cggcctcttg gatatcttcc tgaacggact
     1261 aattcgatca ccgtactagg aaaacctgca ttgaaagatg caacttgttg aggaatataa
     1321 ccgatactta atttttttcc ttcttgattt ttctcagcaa tctttatcat tccttttgaa
     1381 ggtttcagta atcctaacga tgctttcacc aaggttgact tggctgctcc attttctcca
     1441 gttaaaatga caaattcacc cgcatctaca tgataagaga catcttctag tacaggttca
     1501 tcatcatagt aaaaggtcaa atcagatatt tcgatataac gcaccttaca acctccattc
     1561 taaaacttaa ttgatacttt cttttaaagc tttcagattt ttttccatga cagaaatata
     1621 attttcgcct gctttgattt gttcattagt caagctttct aaaggattca acacttctag
     1681 cttcactcct gtttcattcg acaatgtctc agctaccttc gaagaggcat tttcttcaaa
     1741 ataaatcact ttgatgttgt ttttcttcac aaattctttt agctcagcta agcgacttgg
     1801 tgtgggttcc tgatctggtg aaacgcccga aatcgctact tgttttaagt cgtattgatt
     1861 cgctaaataa gcaaaagcag catgctgagt gacaaatgtg cggtttgttg catcttttag
     1921 ctcatcggag tatttttgat ccagtgcttc aagctttttg atataagcat cggcattctt
     1981 cgtaaaagtt tcctttttat ctggatattt ttcgcctaat tcttcagcaa tcgttctgac
     2041 ttcttttata gctaataccg gatcgagcca aacatgtgga tcgtattcat gtgaatgacc
     2101 atgcccttct tctgattctt catgttcgtc tgcttcttgc gattctaaac ggtcgattcc
     2161 tttagctgct tcgatcatca aggtctgttt gctatccact gaatccttta acgaatccac
     2221 aaaaaattcc atgtctgaac tattatacac gaaaacatct gcatcggaaa tttctgctct
     2281 ttcttttgca cttggttcga aatcatgcgg ctccgttcca gcagggatca aaagtttcac
     2341 gtttccttca tcccctacga tctcttttgt aaaatcatac atcggataaa aagtcgtaac
     2401 gatcgtcaaa tcctcttttt tgtctgcttc cttgttcgta tttccacatg ctgcaaacag
     2461 taaagcacct atcattaccg tagctgtcaa aagatatttt ttcatttata tgctcctttc
     2521 aatgaacaaa tcggaatttt acgatttatt aacgtgaact aatgtatcaa aataaaaaaa
     2581 cacagtcaat agaaatccat caatttctga tttaagcatg tatattcttg attaaatcgt
     2641 aaaatggatg agttgctgct catatctagc catttcacta caataaaaaa ctgaagaata
     2701 ccattttagg attcctcagt ttttcatcac ctattttact tcttatattt tattgcaggg
     2761 ttcgtactaa ataaacttcc tcacaaaaac cttttaaccc attatatact ctctgcgctc
     2821 ttgaataatg ttgacacaag gcaaaaacgg ttggtccgct tccactcatc aatgcagcat
     2881 ctgctccgta cttgatcata cgctctttga tttgatggat gactggatgc cgctttcccg
     2941 ttatatcttc taaactgttt cccatttttt tgatcatctg ctgataatct tgctctcgga
     3001 tggcttcagc aagtccgtgg atgtcttgat gttggatggc ttgcgtatct acttctctaa
     3061 acactgtacg tgtagagaca ctcattcttg gtttgaccaa cacaacccag cactgtggca
     3121 tagaaggtaa tacttggatt tcttcccctt tgccaccgac aaatgccgta cttccgtaga
     3181 cacagtaagg aacatctgta ccgacttcca aaccgatttc tgccagttct tctaacgaac
     3241 agcccagatt ccataattgg ttgatccctc ttagagcagc tgcacagtct gtacttcctc
     3301 cgcctaatcc agctgctact ggaatattct tcttaacaaa gatcttgaca ccggttttga
     3361 tattgtattt attttttagc aataaagcag cttgataaac attgttccgt tcatctaccg
     3421 gtaaaaatgc tttatttgtt tctacaatga ttttgtcttc tgttatttct tcaaaaaata
     3481 gatgatctgc caagtctacg ctcgacatca ccatttccaa ctcatgatac ccatcttgcc
     3541 gtctatataa tacatccagt cctaaattta tcttggctgg cgctttttca atcaattcca
     3601 tcctctcacc cattcgaaaa gtctaagtcg caagtattct atcataggga aaaataaaag
     3661 tatagcttat gtctgcctag tggttgattt tgtcatcgtt tcaacagctt tcacagaatt
     3721 ttctcttcat aatccaaaca agttctagtg attcttataa ttctgtgagc acttcttctg
     3781 ccaaactcgg aacatttcct ttttcttttc ctatcagact gagtagctgc atggtagcaa
     3841 atccgaaatt acttcctttc ctataagcaa aataacgtcc ttcccaatga tcgacatatt
     3901 ttaatttctt ttcataagca gactggaagg aatagaaaaa attaccataa ttatatacga
     3961 tatgcatgac tttttcttga aagaaagaaa aatctgtttc tccaacatta gctaatggag
     4021 ctaagcctag attggcttcc gagtagccca ttttctcgta tacaataaac agattggcta
     4081 acagatagtc agctaattcc tcagattctt taggtaagac ccgcaacaaa tcatatcccg
     4141 cctgcttctt cgtcatcggc tgagcggtga tgaagccaac gactttttga tctttacgga
     4201 caataccgac accgctagat aatacatatg atttatcaaa tcgtcccaca gagaagtatt
     4261 tttctcgtgc accttgaagc catttttgtg aaacttcatt tacagcttga tgtaattctt
     4321 ccggcagttg atcgtaccaa gtaaaatggt atccttcttt catcaaacgt tgatattcaa
     4381 gttgctgact cagccgagac aagtcttctg ttgctagatc aacaatccct gcttcaccta
     4441 ctttcgtgaa gtgaaaaccc aattcatgaa gcatcactac gtagctttct gatatcttat
     4501 aaaacgcaag ctgatagcct aaacaatctg cttgtttcat aaactgattc gttgctgccc
     4561 gcatcatttt aggattgcca actggatctc ctaaaacaaa gcatttgttt cctttgatct
     4621 gataaccaaa aacgacttga tcttggtcgt cttcttggta ataaaaataa ctgtaacctg
     4681 acaagcgaag ctgatggctt gtagctgtgc ctccgtatgt ttccaataaa cggtcaaaac
     4741 ggtctttttc gtactcactg ccaagcttcg tagtatcttc agctaaatat tgataaagcg
     4801 ccactaaagc tagcaatgag atgcccaaac caataagccc tgagaaccac acatcatctg
     4861 aaggaaacaa aataaaatga tttcctgtta ttcgaccgac taaaaagcgt ccgctgtgat
     4921 agcctgcaat tgcgtaaacg ataaacaaaa agccgaacaa gctagcatca aagattatag
     4981 ctccccaaga ataaacgaat tttgttcgat aaaattcttt tctggataac cacacagcta
     5041 acagaatcaa gacatatacc aatatcaact gccaagataa cgtacgtgtg attgtattta
     5101 aaataccaaa agatagtaaa gcaatcgtag gcagaaatgc ctttttgact tttactgaaa
     5161 gtgctctagc aaggccgagt aataaaaagc caaccaatag attcactgtc tgatctaaaa
     5221 aattgaatga gaacggcaat aatacttgaa atagccgact gattgcagac agattagtga
     5281 ttgttgatag aagtaccatc atgatccccg caaaatagac agcacttacg agcacgatat
     5341 gcgcaacctt ttggaaaaac agtcgcggaa tgttatctaa aaatcgattg attttgactc
     5401 ctgtatgtgt gatgaacaat agaagtcccg tcataaaagg tagaacataa taaaacaacc
     5461 gataatataa tagccaaact actgctacat cttgccgaac agacaattga ctcattccta
     5521 agatcatcaa aacgtcaaaa gtccccattc cacctgggac catcgtcagc attcctatca
     5581 atgtagcaat catgaacata ggatagatcg aaagcatcga aatatctatt ctcatcaact
     5641 tgccgatgat caaaaatacc gcaagggctc ccgtccattg tccaaaggat gccagtagca
     5701 gaccaagcaa gccttttgga taaaactcga agaacaatgt ctttctcttt agataagcaa
     5761 aaaagaaaat gactggtgct aaaaaactac cgccgaaaag ccagatccaa tagcttcgaa
     5821 aaatactttc tgactgaata aaaaagacat caataaatgt aagaaacgac catacagaca
     5881 aaccagtcag cataaatagt gcgacttttg atacagtggc taaaaccatt tttctatttg
     5941 tctgctttcc ataaaaacta gcccgcagac ttgctcctac cacaccacca aaaccggcga
     6001 gattgttgat cgtgtttgtt acccacgcag caagaagaaa ctcttttctt tccatttttg
     6061 gttttccttg tttttctaag actctgatcg tcacccaatc atacaacaac atcggagtga
     6121 cacccagcag tccagccaga atcatgagca ggatcgtcat gcggctttgt tgtcccatcc
     6181 tttgccaaat atcttgccaa ctcatgccat tagcaatatt tgcgacttga tttgccacaa
     6241 agactaacac ggagccgaaa aaaatcagtt ttaaaatcag actatgttcc tttatccatt
     6301 gcagcatcaa gtatcgttta tttttcacat tttcccattc ctaacttgta tacttttgtg
     6361 tcatacccca atgttatctt atttatttta atttttccac tttagacctg ttctttgctt
     6421 gcgatgatag aaaaagaaag atatcctaat gctaaaggag aggataacat gaaagtagga
     6481 tttatcggca cagggaatat ggcacaagct attattgatg gtttattaaa aaaaggaaca
     6541 gtctcgaaag aagaggtgtt cgtttccagt ggacattttg aaaatgcaca aaaatttgct
     6601 gctgaaactg gcgtccatgc atgcaaaagt aatcaggaag ttgcatcaca atcagatatc
     6661 gttcttttag ccgtgaaacc aaaaatcatt gaaaaagtat tggaagaact aagagaatat
     6721 agccagaaag tcttattcat ttctattgct tcaggaaaat cattaataga tttagaaaca
     6781 gcgcttgaag atcctcaggc ctccatcgta cgtgtgatgc cgaatgtcaa tgtcagcgta
     6841 ggagcaggcg tttctgcgat ttgcaaaaat gaagctgcca cggaggaaca gcttcagcaa
     6901 gctacgatga tttttgaagc aatcggcagc gtctatcctt tagcagaatc agatttcagt
     6961 acattcattg gccttgctgg cagctcaccc gcattcgttt acatgctgat cgatgcgatg
     7021 ggacggactg gtgtactgaa tggtttgtcc aaacagacag ccactgagat tgctgcaaaa
     7081 gcaattatgg gttcttgtca aaaattcctt tcaagtgata aaaatccatg ggaacttgta
     7141 gaccaagttt cttctcctgg cggtacaact gttgcaggtg tagttgcctt agaagaagca
     7201 ggattcatcc cagcaatcat caaaggaatc aatacgacga tcgagaaaga tcttgagatg
     7261 ttgaataaaa aataatgata gcaatgtttt ttcgtgcctt acaaaaaaag aagagctgtg
     7321 gcttttgccg cggctcttct tttgtatttt ttattattta gcataatcag ttgcacgagt
     7381 ttcccgaata acgattactt tgatgtgtcc aggataatcc agttcttctt cgattcgttt
     7441 acgaatatca cgtactaaac gaacagcgtc taaatccgag atttcttctg gcttgaccat
     7501 tacccgaact tcgcgtccag cttgaactgc gtaacttgat tctacaccgg cgaagctgtt
     7561 cgaaatattt tccagacttc ttaaacgacg gatgtagttt tctaatgatt cacttcgcgc
     7621 accaggtctt gctgctgaga gcgcatctgc tgcagctaca aggacagaga taacagaagt
     7681 agcttccaca tctccatgat gagaagcgat cgcgttgatt actactgggt tttctttgta
     7741 cttggcagct aattccgcac cgatctcaac gtgagatcct tcaatctcat ggtcaagtgc
     7801 tttcccaata tcatggagca acccggcacg ttttgctaat tggatatctt cgccaagctc
     7861 tgctgctaag ataccagcta attttgcaac ctcgaccgaa tgcttcaaga cgttttgtcc
     7921 ataactagtt ctgaatcgca gacgtccgat gatcttgatt agatctggat gaagtgtatg
     7981 agcaccgact tcaaacgctg cctcttcccc gtattcgcga atacgttcat ccatttcttt
     8041 acgagatttc tcgaccattt cttcgatacg cgctggatgg attcgtccat cttgtatcag
     8101 tttttccaag gtcatccgtg cgatttctct tcggattgga tcgaatccgg aaagaacaac
     8161 tgcttccggt gtatcatcga tgattaaatc gataccagtc agcgtctcaa gcgttcgaat
     8221 attacgacct tctcgtccaa tgatacgacc tttcatttca tcattcggaa gggtaacgac
     8281 tgaaacagtc gtttctgaaa cttgatctgc tgcgcagcgt tgaatcgcta atgataataa
     8341 gtttttcgct ttgcgatctg cttcttcttt ggcacgctgt tcagattctt ttaccattag
     8401 agttaactca tgattcagct cttcttcagt cgatttcatg atgacatctc gtgcgtcctc
     8461 ttttgataaa ccagcaattt tttctagttc tttttgttga tcttcaatca ttttttcaac
     8521 gtctttttct cgctcttcaa ttaattgctg ttttgaacta agtgcttctt ctttactttc
     8581 aagtgagttc tcgcgttttt ctaacgagtc atctttacga ttcagcgttt gttcgcgttg
     8641 aatcaaacga ttctcttgag attttaattc taacttactt tctctcagct cactttcaac
     8701 ttcagaacgg tatttctgat tttcttcctt agcttctaac aatgcttctt tttttagagt
     8761 ttctgcttct ttttttgcac tatcgatgat tccagcagct gatgattgtg caccagctat
     8821 ttctttctcg tgacgtgact ttgcgaccat aaaccctaaa ccaagaccga caattaaacc
     8881 gatgatagcg aggagaatgt tgaataccat aaaatccacc tccatactat ctttttcatt
     8941 tttatcatct atacagcttc tatgataaat tattctaaac tcgtcaatat gcatcttcgc
     9001 atatatgtgt acaaaaaaca tgcacaactt tattctaatg tttgtacccc gtagtgtcaa
     9061 ctttaaataa acaaagaaac ggctttctac ttattcatta aacgatggtt tttaaaat
//
LOCUS       DAZKLA010000109         8963 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000109 DAZKLA010000000
VERSION     DAZKLA010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.110"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..279
                     /locus_tag="ACG85N_002252"
     CDS             <1..279
                     /locus_tag="ACG85N_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224976.1"
                     /translation="DSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKPVRTRSAK
                     GIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(376..810)
                     /locus_tag="ACG85N_002253"
     CDS             complement(376..810)
                     /locus_tag="ACG85N_002253"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0224977.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1042..1752)
                     /locus_tag="ACG85N_002254"
     CDS             complement(1042..1752)
                     /locus_tag="ACG85N_002254"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0224978.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1742..2602)
                     /locus_tag="ACG85N_002255"
     CDS             complement(1742..2602)
                     /locus_tag="ACG85N_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFQ0224979.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2607..3245)
                     /locus_tag="ACG85N_002256"
     CDS             complement(2607..3245)
                     /locus_tag="ACG85N_002256"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFQ0224980.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3260..3559)
                     /locus_tag="ACG85N_002257"
     CDS             complement(3260..3559)
                     /locus_tag="ACG85N_002257"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0224981.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3602..5086)
                     /locus_tag="ACG85N_002258"
     CDS             complement(3602..5086)
                     /locus_tag="ACG85N_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0224982.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5106..5567)
                     /locus_tag="ACG85N_002259"
     CDS             complement(5106..5567)
                     /locus_tag="ACG85N_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0224983.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5585..6652)
                     /gene="ulaG"
                     /locus_tag="ACG85N_002260"
     CDS             complement(5585..6652)
                     /gene="ulaG"
                     /locus_tag="ACG85N_002260"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFQ0224984.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6870..7640
                     /locus_tag="ACG85N_002261"
     CDS             6870..7640
                     /locus_tag="ACG85N_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0224985.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7913..8740)
                     /locus_tag="ACG85N_002262"
     CDS             complement(7913..8740)
                     /locus_tag="ACG85N_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0224986.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2727 a         1880 c         1401 g         2955 t
ORIGIN      
        1 gacagtgaac tacttaagca atttccacaa gaagaacatt tcatgtataa agaagcaatt
       61 tcccacaaag tgacagagta tttggagcat aatttttttc gtataaagcc agtgcgtact
      121 cgttcggcaa aaggaatata cgagatgaaa gtcaaaatta ataaaaaaaa ttatcgcata
      181 gcatttgctt taaaaaaaaa tcatgtgttc attttttata ttagttcgaa tttgcaaaaa
      241 attgtatttg ataaagaggt gagtaaaaaa ataaaatagc tgagcattgg aggatagatt
      301 gagtgatact aagtctatcc tcttgttttt ctaaagaact aagctagaat tttttctttt
      361 gtacgttatt atatgttatt tgtcattacc aaaaagcacc ttttttcttc tagtatctat
      421 gacataagaa ataccaccca tttcctttac ttgaacaaat cctactgatt cataaaattt
      481 ttttgttttc tcagtatcat ctgtcaataa ttcgatttgg actggtccat aactagtgat
      541 cttctccaaa gcttcattca aaagcacttt gcctattcct tggcgctgat acctcggatg
      601 aatgaggata tcttgaataa atgcgatcgt cgcaaaatca gtgatccaac gaacgattcc
      661 gataatcttc ccttccccat tacgctttat cagagattca gattgttcaa atgctctagc
      721 taaccttgca gtgtcttttg tgtaattcgt ccaaccaacg ctcttatata attcagaaat
      781 ctcatcgatc ataagggaat caatattcat ttttttcttc ctttcttata caattctccc
      841 tctaataata cagttctttg actatttaat ctatcacata taactctcct gtaaagattc
      901 tctcgattcc ttaaaattcc tctttccttt tagaatgcat actttttgat aaataaaaag
      961 gttgtgataa attttcttca caacctctgt ttctctctat caagtgttat cttttaattt
     1021 tctaccctta ccctagcatc tctattgccc gtagtatgca tactttccat gtttgcgaaa
     1081 ataatgttta tctagtaaat attgcgggat ttcacatata ttggggttga tcagctcgtt
     1141 ttctttggcc atcagacaaa tttcatctaa gatcaaactg ttttctactg ctttcatagg
     1201 tgaatccccc catgtgaacg gtccatgtcc atagacaagt actccaggga cttcgtttgg
     1261 atctaatttt ctttccttaa acgtctcgac aatcacattc cctgtatgaa cttcgtaagc
     1321 ttctcttact tcttctttcg ttaattggcg tgtacaagga accttaccat aaaaagcatc
     1381 agcatgagtc gtgccataag ctggcaaatc gcgtcctgct tgcgcccaca tgacggagtg
     1441 cgtggaatga gtatgagtaa tagcagttat atcttcaaaa gtttgataca aaacaacatg
     1501 tgtggggaga tctgaagatg gttttaagct atcttcttct attatatttc ccgacaaatc
     1561 tgttaccacc atttgatctg cttgcatgca ttcatatctg acaccgcttg gcttaatgac
     1621 gatgactcct agctcacggt ccacttcact cacattgccc caagtcaatt tgactaatcc
     1681 gtatcgcggc aatgccagat ttgcttcaag gacacgttgc ttcattgctg caattttttc
     1741 ttcacaagac atcatagcca gcctccttca atttagaact taagtagtct tttgcctgct
     1801 ggatttctct tcgaaagtct tggctttttt cactccacat ttcaatcaga aatgttccat
     1861 cataatctag acgtttcaaa tgtcttaata atccaagaaa atcaacacaa ccgctaccaa
     1921 aaggaacatc acgaaactgg cctgaatatt cttttgttac agggtaagta tcttttaagt
     1981 ggatagcagt gatataatcg atccctttct caagttctcg agccggatta ttttccggcc
     2041 atgcggacaa attgcctagg tctggatata cttgtagaaa aggtgaatga atctgttctt
     2101 tgatttctaa aaattttgaa atggagttca taaatggatc atccatgatt tccacggaaa
     2161 gaatgatgcc atatttagaa gcttccttta cgctttcttt tattccttcg ataaagtctt
     2221 ctctggaatt cattgatttc tcttcataat aaacatcgta tccagctatt tggattactt
     2281 tgacgcttag attatgcgcc aaatggattg cttttttcat tagttgtttc gctgcttttt
     2341 tcttttctgg atcagcagaa ccaaacggaa atcgccgatg cgcacttaaa caaatcgaat
     2401 ggatacgtac accggagctg aacatctctt ctctcagctc agctatcttt tcttcagacc
     2461 aatctaatcg tgctaaccgc tcatccgtct catcaatcga catctcgata aaatcgaagt
     2521 ccatttcttt tacaagggaa aaacgttctt tccatgaaat atctttgggt aacgcttttt
     2581 cataaatccc aatttgagcc atttccttac ccccagatac gaatgatctc atctttgaag
     2641 ttttgtgcgg ccttcatcgg atcatttgct gctgtaatac cacgaccagt gataaaggta
     2701 tacacatcga tcccttcaaa taagtgtaat gtgtgtggat ttaatccacc agttacagaa
     2761 acattaaagc ctagttcaat cagttttttg atcttcgata aatctttttc accccaagtt
     2821 tcaccagata acagcgcatc acggctttga tggtagatta cctgccggat accgatgtta
     2881 tgccaatcca tggcttgttc aaaagtccaa ttgccgtaga gttctacttg caattcacct
     2941 atttcttttt gtgctgcctc catagttgga atcgttgcac aacaaatgac tgtcatccag
     3001 tctgctcctg cttttgcaac attactcgcc actgttcctc cagcgtctgc acatttcgta
     3061 tcagcgacta gttttttatc tgggaacata ctgcgaatac agcgaatcgc tttttgtcct
     3121 tcctgtaagc aaagaattgt gcccacttca ataatatcta ctacatcacc aactttcata
     3181 acatctccta atgcatgttc taagtcattg tgatctaacg caatttgcaa attcggcctg
     3241 ctcatcttag ttccttcctt tacttgtttt tttggtacca ctttgtttca gcaaattttt
     3301 cactgatttc tttgactgac atcacatttt tgataccgat aactgtaact ccttttttct
     3361 ctgcctgagt aaacatattc aaaaaattga ctggcgtaaa aactacatcg tattggccag
     3421 ctgaactttt tccctctgaa atggcacaat gatggatttt ggtaattggt acgtttagtt
     3481 ctttcattgc tttttccaca cttctcatca tcatcaagct tgttcctgat ccatttgcac
     3541 acgatactaa aattctcatc ttaatccctc cattatgagt gttgtctaat cagattttct
     3601 tttatactct ctgcttttct gcatatgtgt catagtcttc tacaatcaaa aaataaccat
     3661 caggattttt ccgatattgt aactgcggaa tagccaataa accaatgaca actacggcaa
     3721 ttccagcata acctaggact ttcatgatta ctgtaaacaa tggccaagct gttgcccagt
     3781 cgaacatccc gatataacca ccgtatgttg acatcccaat ccatgtagcg aataatgctg
     3841 aaccgcctac ttgtatgatt cctgaaagaa acgggaacag gcaagcggct ttgaatccac
     3901 ctcggttatt ggcataaact gctataacgg cattatcaaa gaacaacgga atgaaaccgg
     3961 ctatgacgat cgttggtgat ttcaataaaa tcagtaaacc aatcatcaaa aactgtccca
     4021 aagcaccaaa caagacccca atcgttaccg catttggcga accaaagcca aaagtagcag
     4081 ctacgtcaat tcctggaaca gctcctggta aaatcgtgtt tgagattcct tggaaagatt
     4141 gagttagttc atcgacgaat gtccgaacac ctaattgcaa gatcgccaga tataccgcaa
     4201 aatttaaaga agtcgtcata atgtagaata agaaactctg cccttcttgc atgaattgcg
     4261 cttgaatcaa ataatctttg cctagtacaa tcagaatgat cccaaagaaa aatagcatga
     4321 gtaacgaagt agcaaccata ttctcattga aaatcgacaa gaatcctggt aatttgatat
     4381 cttcaatttt tttgtcttgc ttgattgatc tatttttctt ttcatcccgc tttttcatcc
     4441 actcagctaa ttttgcaaat ataaagattc cgaacatttg ctgatgtgcc actgcaaatc
     4501 cggcaccttc tgtcaattcc tgcgtgattc ctactgttaa gttagaagct actgtccaat
     4561 aacaacctaa aatcagtccc ataaaaatca atgcttgtat cctacctaga tcaggaaagc
     4621 aaaacagtaa aatccaaaaa gcggtagctg cttgttgaac ctgaacgttt ccggtagtga
     4681 aaacggaacg cagtttcgta tatttctgaa aacggactaa taagatattt aaaacaaatg
     4741 caatcagtaa taaaatcatc acatctccaa aggtacggcc aaatgtctcc attaatccag
     4801 catccacagc attttgtcca aaataaggat ctgtaaccat ggcatccaaa ttgaaccgtt
     4861 cctttagtcc aaccaagatt ggccgaaaat tattgaccaa accaccagag ccaacagtca
     4921 aaataaagta accgaccgtt gcctttaaga atccagcgat gcattcatga agcgggcgtt
     4981 tcagcaaaat atagcctaat aacacgataa aaccaatcag gtatgctggc tgcgtcaaga
     5041 tatttgttgc aaaatacgtc caaatgctta acaaaaattc ccccatcttt tttctcctct
     5101 atcaattaat tatattctgc catcacaact tggtaatcat cgatggagtt cgtagagatg
     5161 agacgctcaa ccaatccatc tgtcattaat aattcggata atttttggat attttctaga
     5221 tgttcttctg gatcttttgc tgcgagtgta aaaaacaaac tagccgtttt ctcctcattc
     5281 tccccttcac tttcaaaacg gattggctgt ttcaacttgg taaatgaaat agctgttcca
     5341 aaaacaccat cacttttttc tgaagaatgc ggcattgcta cttgaggtgc gatcacaata
     5401 taaggaccgt atttttctac acaatggatg atctcctcca catacgtttc atcaataatt
     5461 tccttatcga tcaatacaag acagctttcg gcgatagctt cacgccaatc cgtgatttct
     5521 ttatctgggt aatgaatcaa ttgatggtca aaaaagtatt tcagcaagaa actccccttc
     5581 tttcttataa aaacgacgga aacggtgtat cattttcaat cgagaagaca tcttcaaatc
     5641 ctcgataaaa attaaactca agatcatctt tgtttgtcgg atagatgaat ttcccgccta
     5701 cttgccagat ataaggttta aatccatact tcaggcgatc ctttttgaat ttccaaattt
     5761 cagtgatctc ttttggatca gccataaaat tagaccaaat atcgtagtgg acaggaatca
     5821 ctacttttgc attcaatgac tccgccatgc gcagcatatc aacagaagta actttgtctg
     5881 ttatccctcg tgggttttca ccataggccc ctaaacagac atcgattttg tgttcattac
     5941 catgtttggc aaataaattg gagtaatgtg aatctcctgc atgataaaga ttccctcctg
     6001 atgtttcgaa taaatagttt actgcaattt gatccatctc ctgaggcatt tttcctttta
     6061 atacctcttc tggatcttca caagtgacca acgctgtccg atcaaacgct tctaaagcta
     6121 ctacattgat gtctttgatt ttgactgcat cacctggctt tactgtaatc gttttttctt
     6181 caggaattcc ccatttttgc cacgtgcgaa ccacttcttt tggcccaatg aatgttgctt
     6241 caggacaatt ttgatgaact gctgcggctg tatggatatc tagatgatcg gaatggatat
     6301 gtgtaacgat taaagcgtct acatttttta tcgcaaaagg atcgattacg aaaggctgag
     6361 tacgtaaatt aggctgcata ttttgaacgc cactcatacg catcatctga tggccttttt
     6421 tcatcatgcc attcccatga ctacgttttc ccgtcccaca ccatagatca cataaaatat
     6481 tcgttccttc atgacttttc agccaaattc ccgtacagcc cagccaccac atagcaaaag
     6541 ttccaggatc cactacttct gtttcgattt cttcattcaa gtatgtgccc cactcaggaa
     6601 atgtttctac taaccacttt tcttttgtta cttcattgac agttttactc atttgcttgc
     6661 tccccccata tcgttgttcc aacttacaag taaatattaa tgtgtatcag catccatgaa
     6721 aaccctttta tatgaccgtt tgttttttat atgattttct ttcagtaatc aatgattgtt
     6781 tcagtataat aaagacagat atgaatgttt aaatgaacaa ctagtcaaat aaatccatgg
     6841 atcaagatgt ccctcaggag gcaaaagtca tgaaaaactc acttgtcaat attgaaaaac
     6901 gtcaaaaaga aattttggaa ctgcttcagc agactgaaca actatcaact ttcgaattag
     6961 ctgaatccct tgatgtatct attagtacga tacgaagaga tttaaattta ttagaagaaa
     7021 aaaacgagat catacgcaat tacggttatt gttcttataa ttacaaaaac cagactgatt
     7081 ttgatcaatc tggtcctgaa aggattaaac aagcgattgc cagagaagct agtaaataca
     7141 ttaacgatta tgatacgctg tttatcaatt ctagctcgac tgcgttgaaa acactggatt
     7201 ttcttcaatc taaccatttg acagtcatta caaacaacgt gaaaatcaat tactctccgc
     7261 acaaagataa ttgtagttat attttaactg gtggagagat gcgctttcca aaagaagcac
     7321 tagtgggcga catcgcatta aatacgattt cttcagtcaa tgcagacatt tgtatcattg
     7381 gctgtagcgg agtcgatgta gtacatgggg tcacaacaaa gatattaaat gaatcaacga
     7441 tcaacgaaat gatggtcaac aagacaatca aatgtaagat tttagtcgct gatcatcgaa
     7501 aaatcggact gacatcaaag ttcaagattg ctgatatttc agtctttgat tatttgatca
     7561 ctgataaatt ttgttctgaa aaagtattag aggagatcag aaatacgggg ataaaaacca
     7621 ttcaagtaga ttcattttaa gaaccaggac gtaaatatgt aaataggatt atcaaaaaaa
     7681 tgaataacaa atttatcgat aaatgaaaaa cacgctacgc tcaaattgat aaaggaatcc
     7741 caagatgtat ccatccaatc gataccatca taatctattg cttcttttat aaccgttata
     7801 tgttgcctaa ataggaatca aacaatatcg aaaaaaggag gtatgacaaa agtcacacct
     7861 ccttttttcg aataaacggt attcaaatca gaacactgat ttcacaacca ctttatttca
     7921 ggtcgttttc aatcaccttt accgcgtaat catttccgtt gaaacgcgct attttttttg
     7981 cgtccatata tctttctaaa taatcatctt ctcctaattg cttatggcaa gaagcaattt
     8041 cataaagcaa gtattccaat gcataagtcg atttatgttt cttattatac tcaataccct
     8101 tctcacataa ttggattgct ttatcataat cctcgatttt acggtaatag gtggcgatat
     8161 cataatatac aatgcagacc atcagattat caaaatcagt ctcactcaat attttaagtg
     8221 tacgctccac ataaactttc gcaaaatcta attctccttt tgacaagtag atcattgcca
     8281 cgtttgcagc tgcttgtaag tgatggatat ctgtttcggt cgtttgttcc aacacttgat
     8341 taaaataaaa taatgctgca tctggttttt tcgcataaag atgatcaagc agtccttgat
     8401 aaaataaaat ttgatttaaa agatcagaat tacttacttt ttcctgatcg atcgtttgta
     8461 aaattttata agattcttgg tattttgata cagaattcaa tgattgaatc tcatttattt
     8521 ttttcctgat tttgtcttct tctgtttcta ctaatatatc ggagatctcg acattcagct
     8581 ttttgcaaag cgcacttaag atattgatgc ctggcgacat attcttgttt tcgatattgc
     8641 tgacagttgc ttgttcacaa atgccttctg ctaattgtac ttgtgttaac ccctttttct
     8701 ttcggtattc ttttagtaat gctccatcta ctttaatcaa aacaatttcc ttccctttaa
     8761 aaaattattt ctcctttata tcatacacaa aaaaaagttc taaacaacca aaaatgttta
     8821 gaacagaata aaaattttaa ttttaaaata atattcaaat taaacaactg gcgtatctgc
     8881 tccttgattt ctttttgtgt tatctgaata attatcgttt acagttataa cacatagtga
     8941 aaattgtcat ggcgagaaag tct
//
LOCUS       DAZKLA010000110         8856 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000110 DAZKLA010000000
VERSION     DAZKLA010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.111"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..260
                     /locus_tag="ACG85N_002263"
     CDS             75..260
                     /locus_tag="ACG85N_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0224987.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            273..521
                     /locus_tag="ACG85N_002264"
     CDS             273..521
                     /locus_tag="ACG85N_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224988.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            552..722
                     /locus_tag="ACG85N_002265"
     CDS             552..722
                     /locus_tag="ACG85N_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224989.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(824..1291)
                     /locus_tag="ACG85N_002266"
     CDS             complement(824..1291)
                     /locus_tag="ACG85N_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224990.1"
                     /translation="MNYLIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFL
                     VKHDRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLL
                     SPIPLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            1450..1767
                     /locus_tag="ACG85N_002267"
     CDS             1450..1767
                     /locus_tag="ACG85N_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224991.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            1700..2179
                     /locus_tag="ACG85N_002268"
     CDS             1700..2179
                     /locus_tag="ACG85N_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFQ0224992.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            2196..2372
                     /locus_tag="ACG85N_002269"
     CDS             2196..2372
                     /locus_tag="ACG85N_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224993.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            2629..3060
                     /locus_tag="ACG85N_002270"
     CDS             2629..3060
                     /locus_tag="ACG85N_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0224994.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            3066..3383
                     /locus_tag="ACG85N_002271"
     CDS             3066..3383
                     /locus_tag="ACG85N_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0224995.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            3502..4188
                     /locus_tag="ACG85N_002272"
     CDS             3502..4188
                     /locus_tag="ACG85N_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0224996.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            4163..5512
                     /locus_tag="ACG85N_002273"
     CDS             4163..5512
                     /locus_tag="ACG85N_002273"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0224997.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            5503..5976
                     /locus_tag="ACG85N_002274"
     CDS             5503..5976
                     /locus_tag="ACG85N_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0224998.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            5978..6478
                     /locus_tag="ACG85N_002275"
     CDS             5978..6478
                     /locus_tag="ACG85N_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HFQ0224999.1"
                     /translation="MTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATKSGAEFIDID
                     LIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSYNGFDELLAD
                     FVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGPERSRATTVR
                     NDNLPF"
     gene            6493..8805
                     /locus_tag="ACG85N_002276"
     CDS             6493..8805
                     /locus_tag="ACG85N_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFQ0225000.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
BASE COUNT         3147 a         1466 c         1949 g         2294 t
ORIGIN      
        1 aaaagctaat aggtgtcaac gatttagcta attttttagc ttacaaatta tttgtttaga
       61 aaggagctat ttttatgtct gagaatttag acttaaaaat tcgagcggag atgagaaaaa
      121 gaagaatgac tttcaaagaa ctagctgcgc ttatcggtat ttcaggagct tatttatcag
      181 atattctaaa cggcaatcgt gatggaaaga aagcacaaca gcatatcgaa acagtgaaaa
      241 aaatattgga catccgatag ggggggtagg cgatgacaaa actaaaaaaa caagattttg
      301 taaaaaaata taattattct ccatctactt atcaacgtcg aatgtcggaa ctaaaaaata
      361 cagcaatttt ctcagcggcg tatgaacggg tcacaggaca agaagtttgg atcaatacag
      421 aattatacga taaatttttg tctttcaaat cctataacag gttacgcaca agaaaggtaa
      481 cacctaaaga atttatcgag aagcatttag ttgatttata aaaaacagaa cattttgaga
      541 ggtgaaggtt aatgggtaaa ttcaacagag cattagtatt cagcgcaccg ctaatcgtct
      601 atgctttagg gctttgggga agtagacaag cgttgatagg aacgattgtg tacatggttt
      661 ggatttttat agggctggat gaagctgagt acaaaacaaa aaagccagtc gggagggact
      721 gactaatgag taaggacgat attgtatttc tgttaattat gttttgtggg attggatcaa
      781 taataattaa tttgtatttg attattaaag aaaaattttt caattaaaat gagtctttta
      841 gctttaactt aaattttttt tgataattag ctgttcttat gaccattaca agtttttcac
      901 caggtaatat atctttcata tctaaaactt ctgaaaaagc gactgagtag ccaggagaga
      961 ttgcttgtgc caacggtata ggagataaaa ggctactttt atgaatgctt cccatatcaa
     1021 aaccattggc gtgataatgt gaacaattaa ttttcttacc gttgtaaaga tacgtacatt
     1081 cgataattga gtttggcatt tttgatgggt ttgcaattac gccagtaaga atcattcgat
     1141 tacttctatc atgcttaact aaaaaatagg agagcgtaat ttttctttta tctttaaata
     1201 agttatagag ggataaaaaa aatcctgaaa tagaaatgat taaagtaata gcgacttggt
     1261 aatcgctcaa aatttttatc aagtaattca ccaccttttt actcaaatta tatcaaaaag
     1321 gagagaagaa ataatgcaag aattagtaat tttgaaaaat aaaaaaagac acgaacgccg
     1381 gcaagcatga tcgtgtcagg gaaataaact atctttctat attttaccac agaaaggaat
     1441 gaaccgtaaa tgctaattgc aacggataca ctagaccgca tctttttaaa agacgaatac
     1501 aaactgcgca atatagatgc gtcaggaatt ttagtatttg atctttatga caatggaaaa
     1561 attggtatct atcaagcaag tgatattgaa gaaacaaacc ttgctttcga acaaattgat
     1621 gattctgtgg aattggattt agatgaggca atcctagctt ttgaacaaat tgcaaaatta
     1681 ttaaaggagg cacaaaagaa tggcaactct ttaccaactc agcgagtcat atatcaaagt
     1741 cctggaactg gcagaacaat tggatgaaga aattcttcgc gatactctcg attcgattaa
     1801 tgaagcgatc gagtataagg cagaaaactt agcaaaaata gttaaagaag tagaagggaa
     1861 agctgagtta atcgatagtg aaattaaacg tctgcaggaa cgtaagacat cgcttttgaa
     1921 caatgctaag agtatcaagc actatttaca agaggaaatg gaaaagactg gtaaaacgaa
     1981 gattaaaggt gaattattca acattggaat tcaaaataat ccagtatcgg tcaatgtaat
     2041 caatgagaac ttaattccaa aaggattctt tacccctgtg cctcccaaat tggataaaaa
     2101 gcaattgaag gaggaactga agcacggaga tattcctggt gctgaactcg tccaaacaaa
     2161 aggtttgagg ataagatgat gaaggaggag aaataatgga acacgaaaac gtaaaagacg
     2221 ctttgaaagc tgctatcgaa atcgcggaag ctaaaggaat aaaggttgac ggcaaaccag
     2281 caactgtcca tgacattcaa aatttgacga aagaacatct ttattttatc gccgacttgc
     2341 ttggtttgtc ggaattgtat ttagacaaat aaccccacac ggaaggagtg gctagggtgg
     2401 ttgtattaca agtaatagtc tgcgtaacat tcatttggtc gtgcataccg gtttgtatat
     2461 atatcatgaa gaagcttcgc cctgagtctt acaaattata cgcaatttac agcataacag
     2521 tagctcttgc atttacattg tttgcaattt ctaggcttcc gttttaatcg tgggaatgaa
     2581 tttttaatga actaaattat ctataaagca aaaaaaggag aaacaacatt gaaaaacaaa
     2641 ctagcaaaaa caacagcaat catcggacta gcactaggta gcggcgttat cggctacgca
     2701 gcaagcaatg cgtttcaaga tttggacacg atcaaagcaa atttcaacac agtcctacaa
     2761 tacggtcaaa cgaaatcgca acgtgtgtca gaacttgaat cacagctatc caacaacact
     2821 cgcacacagg agcagctgaa agccgaaatt gagcaaatca aatcggacaa gcagaaggaa
     2881 attgaagcca agcaacgaga agtcgaacaa aagcaacagg agatcgctac aaagcaacag
     2941 gaagccgata gcttgcgcca acaactatcc acagtgcaaa acgacaagga gcagttagaa
     3001 cagcgcgtga gcgagttacg gcagtatacg gatcaaaagg taggggagtt ggggaaatga
     3061 aagagttgac tatttatgac ccaaatattt catgggctaa acacacaatt aaagtttctt
     3121 ttatgatttg gggttacaaa ggatatgtta cttacaaagt gggcggtaac actaagggtt
     3181 tatcattaat agctattgat tcagatgatc tatatgatgc taatttcgag gataacccag
     3241 ttaatttccg tgaccttgat gaagattggt tctctatgga acttactaac gataaaggcg
     3301 acagtacact tgtagaagat gaattcgaca gactaggtga ttatattgtc ggtgttgaaa
     3361 ttattgcgca cgaaccagag taggagagtg aatacaaaat gattaaaaga atcactatat
     3421 cagttgaaga agatagtgga tcaacaatgc agcataccga caatcgaaaa acttttttaa
     3481 ccaatagaaa ggagaatttt gatgcagatt aagaaggcga cggatatcga acaaaccaag
     3541 ggaacatatt tgatctacgg tgctccagga aaaggtaaaa cttcaacggc taaatatttt
     3601 ccagggaaaa cactgatcct tgatattgat cggacttcaa aagtccttag aggtgagaaa
     3661 aatgtcgata tcgtatatat cgacaacgaa gatacctgga acgactgggg caacacgttg
     3721 gctgatttaa ctaccaatta tgtaggtgtt tatgacaacg tggttgtcga taatgtcagc
     3781 gaattggaac ggtgtattct gtccagttta ggcgcagaag gaaaaaacaa cggcgttcct
     3841 tcgcaaggcg attatcaata tatgcaattt agaatggtga acagcctacg ctatatgaaa
     3901 aatttagaca gcaaccttat ctggaccgca tgggaagaaa tcgatttgtg gacggattcg
     3961 gatggatctt cttacaacat cgctttgccg cagatcaatc gaaagattcg aaacaatatt
     4021 ttgggattat gtgacgttgt tggtcgattg atggtaaagg aagacgatga acgcggattt
     4081 attttacaag ccacagattc aacctatgcc aaaaatcaat tggataatag atctggctgt
     4141 aagcaaagtg agctgatttt agatggaatt gtacgactac cagaatgatt tagtcgatcg
     4201 tgcaagacaa gcctatgttg atggttacaa ggcaccatgt atcgtatcac cgtgcggttc
     4261 tggaaaatca gtcatggttg cagagatcgc taaacgaacc acacgaaaag gcaacagagt
     4321 gttgttcttg gtccatcgtc gtgaattgat tgatcagatc aaagatacgt ttcaaaagat
     4381 gggcgtagat aatcagcttg taaatttcgg catggttcaa acagtcgtac gacatcttaa
     4441 aaccatcaag aaaccacaac tgatcatcac tgacgaaaac catcacgggt tagcagcgtc
     4501 ctacagaaaa atctatgagt attattcgga cgttccgcga ttaggtttta ccgcgacacc
     4561 catccggtta aacggtagtg ggttggggga tgtgaacgac ttgctaattg aaggagtatc
     4621 ggctaaatgg ttgattgaga atcatagatt agctccttat gaatattatg cacctaaatt
     4681 gatcgataca gccgaattaa agaaagcttc gactggagat tttacaaaga aatcaatgga
     4741 taaggcagtg aagaatacta tttatggcga cgtgttgaaa cattataaaa cgttagcaga
     4801 aggtgaacag gcaatagcat actgtcacag tatcgatgca agtaaacaca ctgctgaaat
     4861 attcaacgat gctggttata aagcaacaca tatcgatgca aaaactccaa aagatgaacg
     4921 tgcagatatt atcgaagcgt tcagaaatca tgaaattaaa atcctatgca atgttgattt
     4981 gatcggagaa gggtttgatg ttcctgattg ttcgactgta attatgttga gacctactca
     5041 atcgttatca ttgtacattc agcaatcgat gagggggatg cgttaccgac cagataaaac
     5101 gtcaatcatc attgaccatg tgggtaatgt gaaccgtttt ggattgcctg atatggatcg
     5161 tgaatggagt ttagatacaa aaaagaaaac aaaaactgat agtgatttat ctatcgttca
     5221 atgcgcgttt tgttttggtg cttacgaacg gccaaaaggc gataatattt gtccgtattg
     5281 cggagagttg cagccgattg aagaacgaaa gtcagagtta gaaatcgatg aatcggctga
     5341 attaatgaaa gtcggagaaa cgaaaattac tttgaatttt gagaacaata aatattacaa
     5401 catgacggaa gatgaagcag aaagtataga ggacttatat gccatagcaa gagctaaagg
     5461 atttaaacct ggttgggctt atatggctgc taagaggaag gggtggttgt aggaatgggc
     5521 ggtatttttg gtaaaaaagc agaagatgtt caaggttcaa taataaatgg cgtaacaata
     5581 ataggtgata ctggaaaaag agatagttca ggcaatcaaa ttgtaatcgc tagaaataaa
     5641 aataacgaag tttttgaatc ttctctatct aatataaaaa gaggtgttgt taacgggaca
     5701 ggcgaagaga gtggctacgt taatttaaaa aaagctaatg aaaaatacca tgttgaagga
     5761 acttacgtta aggctttgtc tgtcggtgga acaaaaggcg ttaattggga taaacgaaga
     5821 aataaatgga aagcacgatt atatttcaaa ggagaatatg tcttacaaaa ggatttcaac
     5881 accaaagaag aagcaatcca agcacgactt gcagcagaag aaaaatattt caaacctatc
     5941 ttagaaaaat atgaaaataa aaaggagaat gattaacatg acaggattta acttagactt
     6001 ttcaaaagcg caacaaggga acgaaatcaa agacggtacg tacgaagtgg tcgtaaacaa
     6061 agctgtagaa aatgcaacta aatcaggcgc agaatttatc gatatcgatt tgatcgttcg
     6121 taacgatgtg gatcaaccgt tccaaaataa acacattttc gcaaaaatct ggaaagccaa
     6181 agctaccggc aaatataacg aaggtatgat catggcaatt gcacaagcgc tacagttgga
     6241 agacggaaaa tcatataacg ggtttgatga attgctggcg gactttgttt tgaaaaccgc
     6301 ctcagtacgc gtgaaaactg aagagtcaaa tggatataaa aacgtcaatg tgaagtcttg
     6361 ggataaaacg aacacacggg gcgtaatgaa tcaccaattc aaaaatggtg atgaaccatc
     6421 cttcggaccg gaacgttcaa gagcgacaac ggttagaaat gacaatcttc cattttaatt
     6481 gaggtgtaat caatgtatga aaaaattcca aaagagctta aaaatctcaa acaatggtgt
     6541 gtttataagc tagtttggga tgaaaagcgg cagaagcaca cgaagatccc ttataacgcc
     6601 aacaacgggc ataaagccaa atccaatgac gagagtacct ggtctgattt tcagaccgct
     6661 ctcgctgcta ttgatcacta cggaatgagt ggactggggt tctttttcaa accaccttat
     6721 ttcggcattg atatcgataa tgctgaaggt gaagttgagc gatttaaatc aggtgatata
     6781 gaagaaaata ttatctatga atttatcgaa agcatgagat cctatgctga gtattctcaa
     6841 tcaggtactg gcatccacat tattgctcgc ggagaattgc ccggcggaag acgcaggaaa
     6901 ggtgacgtag aaatgtacca gaacggccga ttcttcgtta tgactggaaa tgccgcatca
     6961 aaatatttag aaataactga gccgaatcca aaggatatca aacgcctata tgatcgctac
     7021 gttggagatg aaaaaatcat ccaattcaaa gaagaaaatc cgctgatgaa taccgttgat
     7081 ttaccggttg aagaaattat caaacgtgct gaatcgtctt cccaaggggc aagattcaaa
     7141 gtctttatga acggcggttg ggaagctttt tattcctcgc aatccgaagc ggatatggcg
     7201 ttcgccaacg acttggcttt ttggaccggt agagactttg aaaaaatgga tgagattttc
     7261 agagcgtctg caatgatccg tagcaaatat gaccaaaaaa ggggatcggc aacttacgga
     7321 gaatctctac taaataaggc aattgctgat acaaattccg tctacaaccc caaacggaaa
     7381 tctgatttta agatttttat caaagaccag gaacagccaa aagaggaaaa atactattcg
     7441 tacgacgaca ccggcaacgc ggatcggttt actgatatct acggaacgtt agtcaaatat
     7501 tcgtacattg ataaatcttg gtattactac gatgggaaag tttggctaca ggacaatacc
     7561 ggcgaagttc ggaaaatgat cgatacgaca gtagacatca tgggaaaaga accgttaacg
     7621 atccctgaag gcgcagacga tgaaaccaaa gaagctttga tgaaagcgaa agaaaaacac
     7681 gtcaagcgtt ctcgcagcaa tgccggaaaa aacgctatga tggacgaatt gaaacatcga
     7741 ctatccgtat tgccggaaga gttcgacaaa gataaaacgc tgttcaacac acaaagcggc
     7801 tatctgtctt tgcatgacgg tcttctacac gagcatgaga tagacaaaat gtttacacgt
     7861 gtttcaaatg tcgagtacac agaatcggtc gattgtccta tgtgggaaga atttattaat
     7921 cagatttttg acaatgatca agaattaatc agatatatcc agaaatgtgt cggatattcc
     7981 ctgaccggat ctaccagaga gcaatgtatg ttcatcctct acggtcatgg gtcaaatgga
     8041 aagtcggtat ttttggaaat tatttctgaa ttgatgggaa actatgcgat gactatgcag
     8101 gcacaaacga tcatggtcaa gcaaagccaa tcatcagcaa acagtgatat tgcacgattg
     8161 aaaggtgcgc ggttggtcac ttcgtcagaa ccgaatgaag gcgtccgact ggacgaaggt
     8221 ctggtcaaac aattgactgg tggtgataaa gtcacggctc ggcaccttta tggaaaagaa
     8281 ttcgagttcg aacctgaatt caagttgtgg ctggcaacga accacaagcc catcatccgt
     8341 gggaccgatg acggcatctg gcgacggtta aatttaattc cattcactgt ccaaatcccg
     8401 gatcacaaaa aggataaaaa tctgaaattt aaattgcaga cggagttgca agggatcctt
     8461 aaatgggcaa tcgatggttg cctgttgtgg cagagagagg gcttggagaa accaaagtcg
     8521 gttgttgcag cgagtcagga ttatcgaaac gaaatggatc aaataggtac atttatcgaa
     8581 acgtgttgcg aaacaggtcc aggactaaaa atatcaggcg gagaattata caaagtttat
     8641 cgtgaatggg catcagataa tggcgagcat actttcacta acacgaagtt tggcagagag
     8701 atttcaaaga aatatagcaa agaaaaagct ggtggattta tggtttataa aggaatcaca
     8761 ttaaaaccaa gaaaatacga taacgttaga gaacttttta aataaatctg gagggttctg
     8821 gagggttgag aacgaaaccc tccaaccctt agaaac
//
LOCUS       DAZKLA010000111         8830 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000111 DAZKLA010000000
VERSION     DAZKLA010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.112"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(379..876)
                     /locus_tag="ACG85N_002277"
     CDS             complement(379..876)
                     /locus_tag="ACG85N_002277"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFQ0225001.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(916..1725)
                     /locus_tag="ACG85N_002278"
     CDS             complement(916..1725)
                     /locus_tag="ACG85N_002278"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0225002.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2059..2916
                     /locus_tag="ACG85N_002279"
     CDS             2059..2916
                     /locus_tag="ACG85N_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0225003.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2909..3811
                     /locus_tag="ACG85N_002280"
     CDS             2909..3811
                     /locus_tag="ACG85N_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0225004.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3844..4194
                     /locus_tag="ACG85N_002281"
     CDS             3844..4194
                     /locus_tag="ACG85N_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0225005.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4243..5166
                     /locus_tag="ACG85N_002282"
     CDS             4243..5166
                     /locus_tag="ACG85N_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0225006.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5244..6137
                     /locus_tag="ACG85N_002283"
     CDS             5244..6137
                     /locus_tag="ACG85N_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0225007.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6175..6738
                     /gene="amaP"
                     /locus_tag="ACG85N_002284"
     CDS             6175..6738
                     /gene="amaP"
                     /locus_tag="ACG85N_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0225008.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6752..6940
                     /locus_tag="ACG85N_002285"
     CDS             6752..6940
                     /locus_tag="ACG85N_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0225009.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6953..7855
                     /locus_tag="ACG85N_002286"
     CDS             6953..7855
                     /locus_tag="ACG85N_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0225010.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7887..8129
                     /locus_tag="ACG85N_002287"
     CDS             7887..8129
                     /locus_tag="ACG85N_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0225011.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8385..8756)
                     /locus_tag="ACG85N_002288"
     CDS             complement(8385..8756)
                     /locus_tag="ACG85N_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225012.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3072 a         1531 c         1715 g         2512 t
ORIGIN      
        1 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caagtaaaaa cagcttctta
       61 tgtttttact tggctgttct tattactgca gatgcaagct tttgaatacc gtttaaggaa
      121 gaggttgtga aatcagcgtt ttgacctgag tattagctta acaaacggaa aaatagttcc
      181 tcatattttg ccgtttgtta agctaatacc gagggtcagc tgattgaaca ccatttaaga
      241 aagaggtcgt gagcctgccg tttcactctg tgtcataaaa aagccaatct actttgatag
      301 aaaaagcacg taggactaaa acagttctgc gtgctttttt tattgtgtgt taaatatcgt
      361 aaatgaagat agatattttt atgattcgac gatagagacg ttgaaatctt tcaacagctc
      421 taaagcggat tctccgtgtt ttctactgga gactaacatt ggatcaacaa aaaagtctgc
      481 ttcaggaaaa gcactctgca ggcctatcag attagagagt acacacaagt ttgtttcaat
      541 cccgactact tcgatctctt tttgttcttc tttgctttca aataaagttt cctggatttc
      601 agctaaagtt tcaggtggaa tcgtaaagta atacttttta atcactcgtt cattttcttg
      661 tggagaaaga agagggatca attgcaaatc ttctcgttct tcttctttgt ccttcagttc
      721 aactggtata tcccgtgtga agagaatata ttcattattt tggcgtgctt tagctaatct
      781 ttgagcaata cgttctgcca attgatctga ctctggaata taaaattcgc tgtttggatc
      841 aagaatatga ttttgcatat caataataac taacattttc tttcctccta cattcatatc
      901 gtcattttat tctttttaat agaagttttt taaaatataa gcaacaccag cttcttcatt
      961 cgtcagtgtt acttggtttg caatttcttt tacttgtcgc tgtgcattcc ccatcgcaat
     1021 actcaacccg ctgtaattca acatagcaat gtcgttttct tgatcgccaa aagccatgat
     1081 gtcactcgat tcgattccaa ggtaatccgc cagaaaaaca agtgaatgag ctttagtgat
     1141 acctgtaggc gtgatttcta agaaatgcgg atcagagaat actgattgga taccatctgc
     1201 ttgcagatct ctttgtagct tctgtagttt ttcttcactc ttatctgtta atgctaattt
     1261 aagatagcta agagaaggat cttccaacat atttaaaatt tgatcaaaag aatggttctg
     1321 taaattgaaa tgttcataat aagggtcatc tttgactaga tttgattcat cgaatgtcag
     1381 ctgatcatct tgggtttctc ggctagcaat gatggatatt ttcgcttctt gagcatagaa
     1441 gaaggctttt ctcagtgtat cgctatctat ttttgaacgt ctgagaactt tcgccatttt
     1501 tccatccttc atttcagcaa tcagtgaacc attggaagaa atcaaatagc caccgattcc
     1561 taattcggaa aaaatatctt tagcagaaat gctgttcctt ccggtagcga cggttacgat
     1621 tccgccattt tcatcaaatt tccgaagtgc ttggatattt tcttctggta tctttccatt
     1681 tcgatcaagc aaggtaccgt ctaaatcaat cgctgctagt ttcattttat cttcctactt
     1741 tctttttaca tatctatttt cctgttacct taagattaaa tgagaattta aaaaaatgaa
     1801 actaacttgc tttgtcatta tgaatttttt caaaaaacat tcttaagaaa aaggtggcta
     1861 tatatagtag tttttggtca atacctttga ataaaaagcc atgaaaaacg aatagttagc
     1921 gcttaccgat gcgttttgtg ataaaaaaat tttaaaatat tcaatgaaaa caaagcatat
     1981 attttgcttt aaaaccaaat gaggaatatt ctagatgtag aacaggttag aaaaagatta
     2041 gaaaaaggag tgattttgat ggagaagaga tacggcgtag ttcctgtggt aactgcttca
     2101 gacgaaaatt atgctcctta cttgagcgtg atgatcgcaa ctgcattaga aaattgcaat
     2161 aaaacaagac gcataaaatt ttatgtgatt gacgatggtc tatcagaata tagtaaagaa
     2221 ggattggaag aaacggtaaa taaatattca agtaatgcaa gtatccaatt cctgacagtg
     2281 gaaaaagata tctacgaaga cttcctagtc agtgaccata tcacaactac cgcttactta
     2341 agaatttctt taccaaactt attagctaaa gaggattata agaaagtcct ttacttagat
     2401 tcagatgtgt tagtactaga cgatatcgta aaactatatg atgagccgtt aaatggaaaa
     2461 acaatcggcg ctattatcga tccaggccaa gtgaaagctt tggaaagatt aggaatcgat
     2521 tccgatgact tgtacttcaa ttcaggagtg atggtgattg acattgatca atggaataaa
     2581 aaagaaatta cagaaaaaac tatccattac ctcagtgaaa atggcgatcg aatcatctat
     2641 cacgaccaag atgctttgaa tgctgtactt tatgaagatt gggaacaact tcatccaaaa
     2701 tggaacatgc agacttcgct gatttttgaa cgtcatccag caccaaatga aaaatatgaa
     2761 cgtcagtata aagaaggaaa tgaaaaacct tcgatcgttc actttacagg acatgataaa
     2821 ccttggaata cgttgaaaga tcatccgtat accaatttat acttaaaaaa attagcacac
     2881 agtactctaa cgaaagtagg cgagatcaat gaataaaaaa gaaatagcag tagtcgcaag
     2941 ttgtaacaca aaattcgtac cacatttagc tgcattattt gtctcagtat tagataattg
     3001 caacccgtct aaatttgttc ggttctatgt gattgacgat gatatcgatt ttgaaagtaa
     3061 acagctttta cgcttctcag tgaaaaacgc acgaatgaac tcagatgtgg aattcttgaa
     3121 aatcaataaa gaattcttta caaatgtggt aatcagcgat cgtattccag aaacagctta
     3181 ttatcgtatt gcgattccgg aattgttccg cggcacagaa gttgaacgta tcttatatat
     3241 ggattgcgac atgatcgctt tacaagatat ctctaaatta tggcgtcttg acttcggtga
     3301 ttcaatcgta gcagctgttg aagacgctgg gttccatcaa cgactggaaa aaatgaaaat
     3361 ttcagctaaa tcaatgcgtt atttcaactc aggtttgatg ctgatcaatg tgaaaaaatg
     3421 gttagatgaa aatattacgc aaaaagtatt agatttcatc gaacataatc cagaaaaatt
     3481 acgattccat gaccaagatg cattaaatgc tatcttacat gaccgttggt taccacttca
     3541 tccgcgttgg aatgcacaag gctatatcat ggcaaaagca aaaaaacatc caacagcagc
     3601 aggtgaacgt gaatacgaag aaactcgcaa caatccttac attatccatt tttcaggaca
     3661 tgtgaaaccg tggagcaagg attttgaagg gccaacgaag aaatattacg aaaaatatgc
     3721 agggatgacg gctttccgtt gtgtagctaa attccctaaa tatccaaaat atgcaaaagt
     3781 tcaaccaaaa acagatgtag ttgttaaata atagaagaca acaactgaag agggggagta
     3841 ggaatggaaa ttacaatgtc taaaaatgca gtagagacgt taattgaaaa ggttggggaa
     3901 aatacaaaaa tcgcactcgc gcttatcaat gattccgatc catttctaag ggacaaaggt
     3961 gcttttgcaa aaggcagctt ttttcaaatc attccttttg tttcggaatt tggagaatat
     4021 gcgactaaaa tcgagcatcc attattggac atctatacat ctaaattaga gcaaaattat
     4081 ttcggtaaac gattgaacat ggacttcaac aaacagcttg acagcttttc attagaaaat
     4141 gaaatcgcag tactcgatta taatatcaaa ttaaagaact gcttcttcag ctaatagttt
     4201 cgagaaagtc caaaaaggag gtatacattg agatactact ttgtggatga agaaaagaat
     4261 gtaatagaaa cagatgaaaa aaaatataat tggctcgtac ttgatagtgc aaagcccgaa
     4321 gagattgagc aagcaatcga agaattcgag cttcctgatg atattttcgt aggaaccgaa
     4381 tatccggaag aagtttctcg gatggagcgc ctttatgaga cgaaactaga gcatccattt
     4441 tctttggtag tgattaatct agatcagtca gataatcgta tcgaaaaaaa attaacgcca
     4501 atatcttttg tactttccga taatctcttg attacttgta tcgatagaaa aaccgaattt
     4561 gtggatcggc tgctgacaaa gcatggaaat catttaaact cgtttgaaaa gatcatcacg
     4621 tatacattat tagatattta cacccattac gtgaaagaac taagagagat gaaaaagcga
     4681 attgacgctt tggataaagc agctcgtaaa acaacggaaa atgaagaact gtataaacag
     4741 gcagatcttg aaagagacat cgtatatatc gatcatacct taagagatca acgggaaaca
     4801 atggatcaat tatggaattc tgaaactttc atcaaacgtt tagacgatga acagctactc
     4861 tatgatgtcc atttgagaca gcgacaaaca gaaaaaatga tcgagatcta tcgagattta
     4921 ttagagagta ttggtgatct attcaacggg atgatggaca ataatctaaa tcatatcatg
     4981 aaatatctag attcagctgc attaatcatt tccgttccgg cgttgttcgc agggatttgg
     5041 ggaatgaata caggaggatt accgggtaaa agttctgctc taggctttat actcgtagtc
     5101 tttggctctt tgatagcagc agtagtttta ggttaccatt tataccggaa agattataca
     5161 aattaaagaa tctatttaaa acaggtatca aacaaagaaa tttgttttgg caatacaatt
     5221 taaaaactag gaggaataca gcaatgacag aaccaaaatt aatcgacccg cgaaacttgt
     5281 accatacgga gaaatttcct aaacaagatc aggatacacc agctttacag cagaatatga
     5341 ttcctgttcc tgattgtgga gaagaatcct atgaaggcaa taatcaacta gaaaaccgcc
     5401 gtgtactaat cactggtgga gattcaggga tcggtcgtgc agcagcgatt gcatttgcta
     5461 gagaaggggc agatatcgct cttcaattct tcccaggaga agaaaaagat gcagaggaag
     5521 tagcaaaata cgtgaaagtt gcaggacgta agattgtctt actacctttt gatcttcgtg
     5581 acgaacaagc acctaaagaa atcatagata aagccgtgag tgaacttggc gggctagata
     5641 cgatggtact aaacgcagga cagcaaattt cttgtcagtc tattgctgag ttaccacttg
     5701 agcaagtcaa agacacattt atgataaaca tcatcgctat gtttgcttta gtgaaagaag
     5761 ctgttccgca tatcccagca ggaggagcaa tcgtaacaac aacttctgtt caagcattca
     5821 atccaagcga acatttatta gattacgcag caacaaaagc ttcaatcgct aactttacga
     5881 tcggtttagc aaaacagctt gcacctaaag gaatccgtgt aaatggtgta gcacctggtc
     5941 caatctggac accattacaa ctagacgaag gacaaccttc tgattcgatc cctgaatttg
     6001 gtcagcaaag tttactagaa cgtgcaggtc agccaggaga attagcacca gtttatgttt
     6061 tcttagcatc gaataaagca agttatgtta cagcccaaat ttacggagta actggtggag
     6121 aagcgatcaa cttataagaa gttgatcgtt tcacccctat gaggaggtgc gaaaatgaat
     6181 aaaggtgtaa aaacgatcgg agtgatcgga acattacttt tgctatctgt gcttttgttt
     6241 gcgatactat taagtgcaac ctatccattg ccttttcagc tagagaagtt tcgattcttt
     6301 tccatcacta attcatacgt gcaacaatat gtattttggc ttgccgttgc atttgctatt
     6361 ttgacaatta tcacgttact tgttttcctg ttttatccaa aaacaatcgg aaactttcta
     6421 ctgaaacatg gtgatgggga gttgacgttg gataaaaaag caattgaagg aatggttcgt
     6481 tctcacttgc atgaagatga attcatccat tcaccaaaag taaatgtcaa agcaacgaaa
     6541 aacaagatcg ccgtcaatgt aaaaggggag ttgaaacgaa cttcagcact cattggtaaa
     6601 acaggaacat tgatgaaaga aatcgaaaat gaagtacaac acatattggg aacaaaagag
     6661 ccagtgaaag tatcggtgaa atacacaggc tttgaagaac cagctaaacg aagcaatgac
     6721 cgcaaacgag tagaataggg ggggaccaaa aatgaaagaa ctattttcga catacaagct
     6781 ccctatcatc tttggggtac ttggattgat attagccgtt ttatggatca ccgtgggctt
     6841 tttcaagacc ttattgatct tgatcatggc agcgatcggc gtcgctatcg gtttttatct
     6901 gaaacaaacg aaaatcttag atcactattt taaatcttag gaggaaacat ccatgaataa
     6961 caataacaaa aataataaca ataaaatgaa caaccaaaaa ccagtgtctg cacatccaga
     7021 cccaacgaca ccaaaccaat caaatggtgt gaacgatgaa aagaacaaaa cagcaccagt
     7081 atctgcacat ccagatccag tagtccctaa cgttcaacaa ggagtagaag agaagaaaca
     7141 tgatgcagaa aagcatgcac ctcatcatcc aggaactcca gaatctaaag ttccagaaca
     7201 acatgatgca atcgatcctt ctaaacctgt ggaagagcaa aaacatcctt ctgaccatgc
     7261 gaaagcacaa gaaaaactta gttctgacca cacaaacgga caaggaaaac ttggttctga
     7321 tcatacaaat aaccaaggaa agcatgattc tgaacaggaa gattcaatgc gcggtgagct
     7381 gaattatgaa gataaagttg tgcaaaaaat catcggaatc gcaattgaac aagtagacgg
     7441 tttattatct gcaaacggtg gtttcttctc taatgtggca ggaaaacttg tgaatacaga
     7501 taatgtaacg gctgggatcg aaacagaagt cggtaaaaaa caagtagcag ttgatatgga
     7561 tgtcattgtg gaatatggca aagatatcga aaaaatcttt gaagaaatgc aagaagtaat
     7621 cggcaaagaa gtaaaaaata tgacacattt agaagtgatc gaagtcaacg ctaacgtagt
     7681 ggacatcaaa acaaaagaag aatttgaaaa agatcaagaa acggtgcaag acaaagtaac
     7741 tgacgcagcg aaaaaaaccg gtgaatttgc ttcgaatcaa acagataaag taaaatcagc
     7801 agctggaagc ggcgcacaaa aagtaaaaga aaatacagaa ccacgggttc agtaataagg
     7861 aagtaaaaca ttaggaggaa cgaaacatgg gatttatttg gtcacttatc gtcggaggaa
     7921 ttatcggagc aattgcagga gcaatcacga ataaaggttc ttcgatgggg attattgcaa
     7981 atgtcgtagc aggtttagtt ggatcagcac ttggtcaagc attattaggt tcttggggac
     8041 caagtattgc aggaatggca atcgtgccat cattgattgg tgccatcatt ttagtactaa
     8101 tcgtatcatt ctttgctcga aaactgtaaa aagactttta aaaagaggtc gtgaaaaagc
     8161 tgttcttcat caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcatttg
     8221 gttgggcata ttatcgaaga tgcaagcttt tgaacactgt ttatacggaa taagaggctg
     8281 tgacaagact tttgtcacag cctcttattt atgcgggcaa attcccaatt agatttttaa
     8341 aagtttttcc ttaaatgtgt ctttattgat acagagaagc ttctttaata aaacaatgcc
     8401 tccctaaatt ttcttgagat aggaaaggtg aacatacaaa ataatacgac cactttgaaa
     8461 acccagatga tccacaaatg gatcgtatga tactgatgaa gtaaaaagca atggatcaga
     8521 aactcaaaga gattgaaaaa gacaaggaag aaataatatc tcttttttac tcttgtctgt
     8581 tttagccaat aatagctcgt aaagtaatct agacaaacaa atagaagtaa acaaagtttc
     8641 gtaagagcag caatcgtatc atgttgctct gctagacgat tgacgagcag gaaaaatgtt
     8701 ggtaatggaa tatagatcac gagtaatgaa agaaaaaagt aaacaataaa tggcatacta
     8761 ttcctcctaa ttgtgtgagt tcgccctcat tttaaaataa cgatatttgt ttgtgaagct
     8821 ttttcgtgtc
//
LOCUS       DAZKLA010000112         8808 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000112 DAZKLA010000000
VERSION     DAZKLA010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.113"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1388
                     /locus_tag="ACG85N_002289"
     CDS             <1..1388
                     /locus_tag="ACG85N_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0225013.1"
                     /translation="EVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGV
                     IEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPE
                     GTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQ
                     KVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEV
                     EVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVS
                     TAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEV
                     TVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKD
                     SGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGL
                     LGYKNRKKENE"
     gene            1522..2865
                     /locus_tag="ACG85N_002290"
     CDS             1522..2865
                     /locus_tag="ACG85N_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0225014.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2977..4380
                     /locus_tag="ACG85N_002291"
     CDS             2977..4380
                     /locus_tag="ACG85N_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0225015.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            4729..5361
                     /locus_tag="ACG85N_002292"
     CDS             4729..5361
                     /locus_tag="ACG85N_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFQ0225016.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            5949..7388
                     /locus_tag="ACG85N_002293"
     CDS             5949..7388
                     /locus_tag="ACG85N_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0225017.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            7683..7901
                     /locus_tag="ACG85N_002294"
                     /pseudo
     CDS             7683..7901
                     /locus_tag="ACG85N_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            8150..8365
                     /locus_tag="ACG85N_002295"
     CDS             8150..8365
                     /locus_tag="ACG85N_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225018.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8582..8808)
                     /locus_tag="ACG85N_002296"
                     /pseudo
     CDS             complement(8582..8808)
                     /locus_tag="ACG85N_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3099 a         1332 c         1770 g         2607 t
ORIGIN      
        1 tagaagttgg gggcacagta gatctaacag ataatgttac taacttgcca gcgttaccag
       61 aaggaacaac agtaacagat gttactcccg gcggtacaat tgataccaac acaccaggta
      121 attacgaagg tgtcattgaa gtaacgtatc cagacggtac gaaagatacg gtgaaagttc
      181 cagtagaagt gacagacaat cgttctgacg ccgataaata tgaaccaaca gtagaaggcg
      241 aaaaagtaga aattggaggc acagtagatt taacagataa tgttactaac ttgccagcgt
      301 taccagaagg aacaacagta acagatgtta ctcccggcgg tacaattgat accaacacac
      361 caggcaatta tgaaggtgtc attgaagtaa cgtatccaga cggtacgaaa gatacggtga
      421 aagttccagt agaagtgaca gacaatcgtt ctaatgcaga taaatacaat cctgaaggac
      481 aaaaagtgac tacagactta aataaagagc cagatgcatc tgagggaatt aagaacaaag
      541 aagatttacc aaaaggaact acttatgctt ggaaagagaa agtagatgtt agcacagctg
      601 gaaataaaaa aggtactatt gtagtgacat atccagatgg atcatctgat gaagttgaag
      661 tagacgttac agtgacagat actcgttccg atgcagataa atataatcct gaaggacaaa
      721 aagtgactac agacttaaat aaagagccag atgcatctga gggaattaag aacaaagaag
      781 atctaccaaa aggaactact tatacttgga aagaaaaagt agatgttagt acagctggaa
      841 acaaaaaagg tactgtagta gtaacatatc cagatggatc taaagaagaa gtagaagtta
      901 ctatttctgt agtagataaa aaagcaccga ataaacctca agttgatcct attacagagg
      961 gtgaccaaat tgttactggt aaaactgaac caaatgcaga ggtgacagtt acattaccag
     1021 acggaagtca acaccatggg acagctgata aaaatggtaa ttttacagtt aaagttccta
     1081 aattagaggc agggacaaaa gtgatagtaa ctgcaactga tgaatctggt aatactagtg
     1141 aacctactaa tgtagttgtt tcttccaaca aaaaagacag tggaaaaaac ggtagtaaag
     1201 gtagtaagac tgacaatcaa ggtagcaaca gcaatcaaga caaaaataga ggcaaaagcc
     1261 agtcctcaaa agtttttcct aagacgggag agagtgacag taatatcttt actattagcg
     1321 gtggtttaat actgttagga acgttaggat tattagggta taaaaatcga aaaaaagaga
     1381 atgaatagaa aatcaatttc ttaagtcttc tctgagaaga ggaagaatct ttttcttcct
     1441 ctttttgctt ttttatgatg ggaaataagt gttagttaaa gatgaatata ctttttatag
     1501 aaaggaaaag aatgtaatta agtggaaaaa atagtaatta ttggttcaaa ccattcaggg
     1561 ctagccgcag ctcgatttct aaacttaagc gattcaaatt atgaagttat tctaatcgat
     1621 aaaaaatcag aaattagtta cttggaatgt gggacatcgt tatttataaa aggagaagta
     1681 agtaatttag aagatctttt ttatcaaaat agaatgacac taaattctga gataaaaaag
     1741 ttatttctag agacagaagt tgacaaaatt gatattcaaa ataaaaagat tagttgtgta
     1801 aaaaaagatg gtacaagatt ctctcaagaa tatgacaaag ttatattagc tacaggttca
     1861 aagcaaatta tgctagatat accggggaat aatttagcta atattttttc gataaagaat
     1921 tttccaaatg caaaagagat ttttaaagct ttggatgata gtactattaa aaaagtagga
     1981 attattggag caggatatat tggagttgaa attgctgaag ccataagaaa aagagggaaa
     2041 gaagtatatc ttttcgatgt tgcagataga gtgttgtcga cttattacga ccgtagtttt
     2101 tcagataaag ttgaagaaat actatcaaaa aatggaatac atctttgtct atcagaaagt
     2161 cctaaaaaat atttaggaag aaaaaagatt gaaaaaatag tcactacaag tggtacccaa
     2221 tactctatgg atatggtcgt acaagctata ggatttctat ctaatagtcc aattggggaa
     2281 aaggaactct tgagagatgt tagtggagca tatctaacta atgaatacca gcaaacaaat
     2341 ataaaagata tatatgctat tggggattgt gctacaactt tttttacgtc cattaataga
     2401 agtagtgttg atttttctat ttctaatgct ctaagaacag cttatattgc aactcaacat
     2461 attaacaatc aagattttac accttctggt tcccaattta caaatggctt ttctatattt
     2521 aattacaatt tttacgcagc aggtttgaat ttaaaaacag cacgattatt aagtgagtct
     2581 atagattata tagaaataga agatttcatt agacctgctt ttttgagaaa aaatagtcgt
     2641 gtaaaattaa gaattagctt tgaaaaaaat acaaagagaa tagtaggtgt acaactttgt
     2701 agtcaagaag atttatctgg actattatca atgttttctc ttgctataga agaacaaatt
     2761 acattagaca aattgaaaat gttagattat ttatttcatc ctagttttag ccaaccctat
     2821 aattttattt taaaggcagt aacaggatta aacattgaaa cataatcgtt actaaatgaa
     2881 atcaagaaat atactgtatt actcaattca ttgaaaatat ttatcataat ttttaataga
     2941 aaacactatt aaagagaaaa aagagaggta aatcaaatga aaaaatcttt aaaacttgca
     3001 tcagcagtta tattaatgtc ccaactcctt atagcaacac ctttccatgt gttggcaagc
     3061 gaaaattcaa catacaaaat tgcaaaagaa agaaccgaga cgactgaaat gagtaattcc
     3121 gatgtaacag cagagagtag tgaacttttg acaacttcat caagtgaaag tagtacgact
     3181 tcaaatgccg agtcttcaac aacttcatca agtgaaagta gtacgacttc aaatgccgag
     3241 tcttcaacaa cttcgtcaag tgaaagtagt acgatttcaa gtaccgaatc ttcaacaact
     3301 tcaagtagta caagtgaaat aacttcttcg aaagtgacaa aaccaaacca tatagaatct
     3361 tcaaaaaagg aagcgccaaa aacggaagga caaacaaaaa ctttttcgac accacagcaa
     3421 atagaatcaa cagaatcttt ccaatcacaa ccaatactat ctggtaatga agaaattcat
     3481 tttgacaaaa atgaatctgt agaaaatttt attatgaaaa ttggtgagtc tgcgagaaaa
     3541 attggtcaga aatatgattt atatgcttct gttatgattg cacaagcgat cttagaatct
     3601 gccagtggtc aaagccagtt agctcaggca ccgaattaca atttatttgg gataaaagga
     3661 acttataatg gaaactttgt cattatggta acaaacgaag atttgggtaa tggaacgctt
     3721 tatacgaccc aatcaaaatt tagagtgtat gaaaactatg aggaaagctt tgaagattat
     3781 gccaaattat taactaaggg aattagtgga aataaagatt tttatgcagg agcattaaaa
     3841 gctaatagta aaacatatcg agaagcaact aaattcctta caggacggta tgcaacagat
     3901 acacaatact atttgaagct aaatgaatta attaaaacct acgatttaac caactatgat
     3961 aaagaagtta aaggtccaca aatgagtaag actggatacc aagtgcctat tcataattat
     4021 tcaataacca gtttgtttgg aaatcgtgga aatgaatttc accgaggact tgatatggca
     4081 gctacacagg gtgagccgat tcatgcagtt aaagctggta aggttgttaa agcagagttt
     4141 cactactctt ggggaaatta cgtggtgata caacacgaag atggatcaac cgcgttatat
     4201 gctcaccagc aacaatacct agtaaaaact ggagataatg ttttgcaagg acaaataatt
     4261 ggctatgtag gaagtactgg gaatagtact ggcccacacc ttcatttgga aatttgcaaa
     4321 gatagtagtc tttctcagag catgttagtt gatccgaaaa ccttattatt cggagactaa
     4381 aaaaggtaat caatctgaca aagtaacaag aaaatggttg gattcttaga cctttgatca
     4441 ggatattgag actggaattg atatctcgat tacgatgggt ttgacaaata aaacaagtcc
     4501 attatcgaat atcgagtgta ttattgccgt ctttatgctc atattttgag caggtttcac
     4561 tagatgaaaa caatttatct atcttgagga tttcgtgtac gtatcgttca gtcttgtatt
     4621 gtaaattagt cataaaatca gactaagaaa aattggaaat atttttttct gatttattaa
     4681 aagttaattg aaattatatg gtacttcttt cttgggaggg taggtgcagt gaaggaaaga
     4741 tatggtcacc ttactgttgt tgaggagatt actcataatg gatataaaaa atatcgttgc
     4801 ctatgtgact gtgggaatga aacgattgta tatcgatcaa acttactatc aggtagaacc
     4861 aaaagttgtg gatgtggaaa ttataaaaat aggcataaat ataaaaattt gacaaataaa
     4921 acttttggta agttagttgc aatcaagcca actaaactgc gaagtgaagg aactgttgtt
     4981 tgggaatgtc aatgtctgtg tgggagaaaa aagttagtat cggcaaagcg acttctaaaa
     5041 ggtgatgtaa aaagctgtgg atgtttgaaa tcaaaaaagc atcacattga aaataaaaaa
     5101 ttcggattac ttactgcaaa gtatcctttg gacaataact gttcatggaa aacaaaatgg
     5161 gtttgtcatt gtgagtgtgg cagtatgtgt gagataagtt atagtaactt attttttgga
     5221 catactcaaa gttgtggatg tttaaaaagg caagaaacaa ggacatgtgt tgaagggaca
     5281 gtaatagagt gtctaacgtc aaaattaccg aaaaataata caagtggtgt taagggagtt
     5341 tatcgttcta aagagaaata gattgcgtat ataactttaa aaaaaaagag gtactattta
     5401 ggatcgtatg attctattaa gagtgcagct caagctagaa aagatgctga aaataaattg
     5461 tttcaacctt tgattgacaa atatcgaggt aatcattaat tattgtggat aataattaac
     5521 agtgatatat aaacatctat aatatattat tcatactatt gatatttagt ataatcctat
     5581 ggaattgaat atcatagcaa tgtatttgaa aagcaaattg gaaaacaaat aggtgcaaca
     5641 ccataaatat gaaaaaattg ggaaaaagta gaatggaaat ttgcttacgt ctcacgagat
     5701 agctcacact attaatctga agaacaatga agtcgacttt tagaaattgc tagaagaaag
     5761 taaagcgaat tatcactagt tactatagag taagttcaaa taaagcatct ccaacttaca
     5821 tggttacatg aggatatcaa atcgaaatta ctcaagcttc ctatggctag agtcgcgaga
     5881 agttgttggg cagtagacat aggttatatt gttcttagct acacgatcat tagaaaggaa
     5941 atcctattat gtatagttat caagaagatg caaaagtcca aaaaaatcgc tggtggattt
     6001 tgattgctat atctatgttt accttcatgt ccacattgga tggcagtatt gtcaatattg
     6061 cactacctac catctcagat gatatgggca taccaatgaa tcaagcggaa tgggtagtgt
     6121 ccgtttatct gatgattgtc tgttcttgtt tgttgctgtt tggtaaaatt ggcgatagcc
     6181 gtgggaaaat caaagtattt cggattggta cgctgatctt tgtcttggga tcgctgttgt
     6241 gtgggttcaa tcattcgcta ggtattttat tgttcgcacg ggtggtacaa gccattggtg
     6301 ctagtatgac tatggctaca aatgccggaa tcatcacgga agtctttccc atacatgaac
     6361 gtggtcgggc attgggagca ataggtgcgt ttgtttcatt aggctcgatt gctggaccag
     6421 gtcttggcgg cttgattttg tcccaatttt cttgggcgta cattttttgg atcaatgtac
     6481 cggtgggaat cgtaacgatg ttgattgctg aaaaattctt accaaaagat atcaccatga
     6541 gcggccatac aatcgatgga gggggctttg ctgccattgc ggctacgatt atcagctttt
     6601 ttggagcaat ctttttgggg caagaaagtg gctttttcca tccagtcccc cttgtactgt
     6661 tcgtaatttc agtgattgcc tttgtcagct ttttaaaagt tgaaaagaaa gcagcctctc
     6721 cattgatttc tttcggtatt tttaagaaca agatttttac catgagcttg attacagcct
     6781 tgttgatttt ttcttccaac ttctttgtca atgtagtgat tcctttttat ctgcaaaatg
     6841 ctcgcgggtt gagtcctagc tatgctgggc tgttgatgat ggtctttcct ttgatgatgg
     6901 tcgtcggggc gcctttaagc ggctacctga ccgaccgggt tggcccggag attttggtat
     6961 tagtaggctt gggcttattg tcggtaaccc aggtcatgta tatgtttttg tcagtggact
     7021 ctcctttgtg gctatacatt gtcgctactg ccatcatggg tgccggcaat gccttgttcc
     7081 agtcccccaa taatacgatg gttatgagct cggtcactat cgaaaacttg ggactggcag
     7141 gctcgatgaa ttcctttgcc cgaaatttgg ggatggtcag cgggatttcg ctttctacga
     7201 cggtcttata tcaagcgatg tctgctaaga tgggccagca agtaactaac tatatcgcaa
     7261 atcggccgga tgtcttttta tatggaatga agattacctt tttaggcagc tttgttttgt
     7321 gttttgttgc ctttttcctg actatctggc gtatcaaaaa acaaggaggt attcagaaaa
     7381 atgtctgaaa aaaccactgt tgaagaattt ttggaggcct gcgtatgggt gagtgggatt
     7441 tccatggatg atggaagatg aagtgaactt ctaaaagccg aaaaactcaa gtattatctt
     7501 caaaatacta gtgctcaatg agatgcttga tttgatcaca agaagactgc gaaagccgct
     7561 ggcaaccgca agctaggtat cccaccgatg gactacgttt tagaggctac tggttatgaa
     7621 tacggagcca acgctctcat ctgcataaga atacatcacc cagattatgt aaagattttc
     7681 gatacttctg tcaaaaaatc attaagatag ataaatgatt tccgtctagt caaattggtt
     7741 cagaatgtag acacacagat ggtaagaagt cactcacaat tcgagaatgg acttgtccta
     7801 tttgccgtat acatcataat cgaaacgtca acgatagtat caatattttg accaagggct
     7861 atgaatacaa gcattgcctt agaaaacatc aagaacctta ggaacaacgg ggatagcttg
     7921 gttaataaga gataccgctg tttaggcttg caagtatact cttttatcaa gaattccgtg
     7981 gctttagtca tgagaggctt aatactatga aaattttctt tagcattttt gatttaggtt
     8041 ccttttctaa ttataataag atgaccacta cagtaaccta ctgaaatata taaacagcca
     8101 ctgtagtacc attttcaaag tagtctatga gaggagaatc ttcattctta tgaacgctgg
     8161 aattgcggat atgaatttaa taaaaaaaac ccttaatgat tttacttcca attcaatatc
     8221 aaaaggtact ggtatcaatt taagcacaat aaaaaagctg aaaagtggtg aaagatcagt
     8281 ggaaaagctg aatcttttag atgctataaa aataacggaa tttgctatga aaaatggaaa
     8341 agctgagatt gaaatatgga gataattatc gtctagtgaa ttacttgcca cttagctgaa
     8401 tttaacgatc cttggtgctt gaagggggag attcttggga atagagcata cttgcaagcc
     8461 tatgtcttta ctaacaacga tatctcttat taaccaagct atccccgtag ttctaaggct
     8521 tttactttta tctaagcaat aatcgtattc aaaggaaatt tttgaccctt agaaagtctc
     8581 attttatcag acgtgagctt aggcattgct gaattttgtt aattcggttt taggttgtgc
     8641 tttgagcata atatgaaagt attctttatc acgatttcat tcatctaagg taatgtgatg
     8701 tgattctgaa attctttcaa aggcagtttt tgcataagct gacattacat catcaatgac
     8761 atgtttacaa tgtttcacta ctaaaataag ggggtaacca agaagaaa
//
LOCUS       DAZKLA010000113         8665 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000113 DAZKLA010000000
VERSION     DAZKLA010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.114"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..275)
                     /locus_tag="ACG85N_002297"
     CDS             complement(<1..275)
                     /locus_tag="ACG85N_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225019.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLF"
     gene            complement(393..653)
                     /locus_tag="ACG85N_002298"
     CDS             complement(393..653)
                     /locus_tag="ACG85N_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225020.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(790..1203)
                     /locus_tag="ACG85N_002299"
     CDS             complement(790..1203)
                     /locus_tag="ACG85N_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFQ0225021.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1659..2144)
                     /locus_tag="ACG85N_002300"
     CDS             complement(1659..2144)
                     /locus_tag="ACG85N_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225022.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2283..2498)
                     /locus_tag="ACG85N_002301"
     CDS             complement(2283..2498)
                     /locus_tag="ACG85N_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFQ0225023.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2722..3522
                     /locus_tag="ACG85N_002302"
     CDS             2722..3522
                     /locus_tag="ACG85N_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0225024.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3541..5409
                     /locus_tag="ACG85N_002303"
     CDS             3541..5409
                     /locus_tag="ACG85N_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0225025.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5402..5893
                     /locus_tag="ACG85N_002304"
     CDS             5402..5893
                     /locus_tag="ACG85N_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFQ0225026.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5881..6435
                     /locus_tag="ACG85N_002305"
     CDS             5881..6435
                     /locus_tag="ACG85N_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFQ0225027.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6453..7448
                     /locus_tag="ACG85N_002306"
     CDS             6453..7448
                     /locus_tag="ACG85N_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFQ0225028.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
     gene            complement(7590..>8665)
                     /locus_tag="ACG85N_002307"
     CDS             complement(7590..>8665)
                     /locus_tag="ACG85N_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0225029.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         2985 a         1409 c         1663 g         2608 t
ORIGIN      
        1 aaaaatagca caatggcaaa gatcgtaaga gagatcataa gccccaatga atgactaaaa
       61 agaaaagcag aatcacatgc catatcatcc catatttctt caagaaaata aataagcggg
      121 aaaaagggaa ggaatagtag aacatcgatc cagcaccatt tttttcttct tttgaaataa
      181 cataaaacaa taaacatgaa cgtgcaggga agtaaaaaca aagctttact cataactaac
      241 aaaataccag ctccttttat ttttttctcc cccacattca aagagtaaca atggtatgta
      301 cttaataata gaaaaatgga tcaacttgta tgaataacag ctgaaaagat ctgcaccaaa
      361 taaaaaactt ccagccaaaa ctgactggaa gtttattttt ttctgaaata ttctaacaca
      421 aatatgaaaa agatggccga agctaataag ccgatttcgt agcaaaacaa tgagatacca
      481 gagtcgtaat aataaaatcc tctaatctta tggataaaat agatcatcgg acaagcagat
      541 aagagtaagt atttgttagg ccttttgaat aagtaaaaga aagcactggt cattatgata
      601 aatggtatcc aaaacaaaag tttacttaag acaatcaaat gatcaactcc catatttttt
      661 accaagagag atcataacat ctttatcgta aaaacaaaat atttttttgc tcattttgaa
      721 aatttaaaca tactcgttct tatccaaata tataaaaatc gcccctcatt catcgagagg
      781 cgataagttc taatttaact caattttttt tgtttctttt ggctgataag ctgttttagg
      841 taataccaat ttcagcacac cgtttttaaa ggttccagtg attttttctt catcaacatt
      901 aggaagatag aatgaacgtt tgtaagagac cgaatgtctt tctttccgta catagttgcc
      961 atcttcttta tcttctgtat gggactcatg attggcggag atcgtcaaag tattatctga
     1021 atattcaact gtcgtgtctt ctttgtcaca gccaggcata tcagcagtta gtttgtattc
     1081 tttttcattt tcgactaaat ctacttttgg ataacttgaa acacctaaaa aatcattgaa
     1141 tgctggacta aacacgtcat tgaaatcagg gaacatgtcg cgaatgctag gtacatttgc
     1201 catttttgat catcctttca ataagtattt tttatttatc cctatttacg taataactct
     1261 attttgaaaa aaatgcaagt ataatggtta gctttgtgaa tatttcatct aatacgatac
     1321 aaaggctatc tttctaattt gaaaaaggga tcattaaaat aataagaaga aaatatgtca
     1381 aaaggtaaaa aagtaaattc ttctatccaa atatagaaaa attgaatcta gaaaaccgcc
     1441 cgacaataaa tttcattaac tcagttgacc gaaaatgaag tagataccaa aaatttatca
     1501 agagagcaag tagagaagtg agtatattca taagagaaat agctgtaacg aattattagg
     1561 ttaatgcaaa tgcttattta tactccgtat aactttctca gaaaaaatga taccaataat
     1621 gacttgctag tactgtaaat ccgtataata tcatctaatc aaaatggtgt tccatttgca
     1681 tctgtcatcg taatcgatcc ttcgggtgac actcgataaa aaccaacgct tccagatcct
     1741 ccttgggaac ggatttgttt gctggaagct gtgaacaaat aatctcgatc aattttttga
     1801 taaaagctaa taacaatatc gtcactttgt aattcggaac ggtgggcaat aagaaataga
     1861 agtgctttgt cagagttttc taatggaaga gaaggtacag tgctttcttg catttcagat
     1921 gttgaagaaa tgctaaacga ctgaatactt gattggctat gttgcgaatc tacattacca
     1981 gtagaagtat tagtaatgga agtagtagaa gtacttttag ttgaaccaac ctcagttgtt
     2041 gaaatggctg ctgaagattc tttaacagaa ttctttgttt tatatccaca ggcagaaaaa
     2101 agtattacag ttcccaacaa gcctgcgtaa atccctttat gcatgatagc tcctcctaaa
     2161 atagattatc tttattcagt atatcactta aacaaagaaa agcagatggt ttaaaatgaa
     2221 aaaaccttcc caataaaggg aaggttcatg aagataataa cttttttaat aattatagct
     2281 ttctaatatc tttcatcaac ttttctagta atcattggga aaaatttcaa gcagtagatc
     2341 gcacaaatac ctacgacgat ataaatgatc ttcgcaaaaa tagttgttga accaccagca
     2401 atcatagcta ctaaatcaag ttcgaacaag cctaccaaca gccagttcaa ccctcccaca
     2461 atcaataaag ttaatgcaat agcatctaaa gttttcatta gaattctcct ttcttaaatg
     2521 ataaaacaat tatatatcaa aaagagaaac attgcttata taacgctcag gaattttagc
     2581 aatctatgca aaaagatgaa aatttttttt gctcttccat tagtacacat tttttagctg
     2641 aagctgtaaa tttcatgtct taattttttt tagtgagctt ttatactgga tttgtaagaa
     2701 aaaagaagga gaggatcaca tatgtcggat tggttggata ttgcaggaaa agtagtaatt
     2761 gtaactggag gttcctcagg aattggaaga agtatcgtcg aaaatttgtt gaagcagaat
     2821 gcacaagtag cgaattttga tgtaactgaa tgccgtatac agcatgaaaa cctactatct
     2881 ttaaaagtcg atgtgtcttc taaaacggac atagaagaag ggatatataa agtaatgaaa
     2941 catttcaaga cgatcgatgg tctagtcaat aatgcaggaa taaatattcc ttctcttcta
     3001 atagatagaa atcatccaaa aagcaagtat gaattatcag aacaagtttt tgataagatg
     3061 atcgctgtca atcaaaaaag tgtatatttg atgagccaag cagttggaag aatcttggtg
     3121 caaaagggat caggagtgat cgtgaatctt tcttctgaat caggattgga aggatcagaa
     3181 ggacagagct gttatgctgc tacaaaagca gcgatgaaca gttttactcg ttcttgggca
     3241 aaagagttag gaaagaggaa tgttcgtgtg gtaggtgttg caccagggat actggaagaa
     3301 accggtttga gaacacaaga atatgaagaa gctctttctt atacgagagg tataagcgtc
     3361 gaacagctga gaaacggtta cagtcgaaca agtacgatcc ctctaggaag gagtggtaag
     3421 ctgcaagaag tggcggatct agtctgctat tatctttcag aaagatcaag ttatatcact
     3481 ggagtcacga ctaatatttc aggaggtaaa acaagaggat aaaggatagg ggggacgcac
     3541 gtgacgctag taaacagatg gtaccaatta ttgaaattac tagtcactca caagaaagtt
     3601 tcaatccagg aagtgcaaaa agaaatcaaa accagtaatc aaaccataaa aaaagatatt
     3661 ggggagttga atgaagaaat tgccggtatt gcaaaaatca tccaaaaaaa caaacatttt
     3721 gaacttgaaa tttatgattt tgattttttt gatgagatca tgcaaggaaa attgaaaaca
     3781 gccagtgatt tcaattcggc atccaaaaga atcgcttatt tactaaatag gctgatcgaa
     3841 acagaggatt tcttacgtat ggatgatttg tctgaagaag tcggtgtcag cagaggaaca
     3901 gtggtcaagg atctaaagac attaaaagag atgatcaatg attttgatgt aaagattaca
     3961 ggcacaccaa ataaaggact aagaataata ggagatgagc tagaattacg actactttta
     4021 ttttattttg tttctcccta cttttcagag acgaaaacag aacagtttct agaagaaaaa
     4081 caagcaatca aacattttca gaagaagacg aatgctagta aacaagaaat caccctttta
     4141 acgaaagtta tcggtatttc tttgaataga atctctagta actatctgtt gaagaagccc
     4201 atcaatcgtt acagtgggtg ttttgaatat atccaagaat tcaatgaact gatcaatcag
     4261 ttggaagaaa tctatgaact aactttgagc caatatgaaa aagattttat cagttttcct
     4321 ttgaacatca tcaacacaag tataacgatg ataaggaaag ctaactctga tttgcgctta
     4381 tatttcgacc ccatgatgat gcgtattcga cgattagatc tcattgatct ggatgagtcc
     4441 ttcttatatc aagagatgaa acagcacctc tctttattga taaatcgggt aatttttcgt
     4501 tatcgattga ctgatttatt ttatggtgag atagaaaaaa agtatccttt ttcttaccaa
     4561 atcgctagcg aatgcataca agcattagaa gctatcttgc aacataagat ctctaaagta
     4621 gagatcagct acctaacgtt gtatttggag ttagcatttc gatatcaaaa agagcatttt
     4681 tcctctaaaa aagtagccat tgttcatacg acaggaaaag gaacagcatt aatgatccaa
     4741 agacaaatca aaaggatatt ggggagggat atacaaataa cacatgtttc agaagaacaa
     4801 tatgaaaaag tagatctaaa caaatatttc gctgttttta caacgattcc gttaaaaaat
     4861 gtaaatgaag agacaccggt gatccatttg actaatcttt ttgatgactc ttggttaagg
     4921 gcacaatggc aaaaagcaaa aaaaaagcga ttagccgggt ttcattctac tgtttttttc
     4981 tatcaaatat ggaaagaaaa aaagacgtat caagaatatt taaacaaatt agtcgctcct
     5041 ttgatcagag gctattatgt ggatgaagaa tttgaaaaaa ggatcttgaa aagagaatat
     5101 cagcaaagca catttgttaa caaagaattt gcttttcctc acacaaccaa ccagctatct
     5161 caacacattg ttttatcagt agggctaata actgatcaag gtttagcaga aacaggagga
     5221 aatggagtta tttttttatt tgccatacct gaagaaatga cggctgaagt tgagaatgaa
     5281 ttatttgagc tatacgatat cctatttgaa ctcataaaaa tgggaataga tgagtttatt
     5341 gaattagcac cgacacaaga atcattttta aaactaatca aaagtaaagg agagagactt
     5401 catgaatgat atgtatctat tcatagtttt cgcagcttca gcttatgtgc tgcaagcagt
     5461 tatcagttgg aagcaactaa agcatttcaa tcaaacatat agcgaactgc gaagaaaagg
     5521 aaaagtagcg attggtaaac gacctgggaa aatccgttca ggcacgattg ttatgctagc
     5581 tatagacgat catgatcaag ttttagaagt cagaaaaatg caaggggtta catctttggc
     5641 aaaatttcat tcattacctg tctatgcggg tgaagatatc cattacatcg atcgttacca
     5701 tccctcagtt cgagcggaag atcgtttgac acaagcagct ttagaaaatg ccagagaagt
     5761 atacttacga atagaggcag gaaatgaaaa aagtgaacaa ccttcattgc cttttattgc
     5821 tatgctcaat aattttcgga tatggaaaag tacgatcaaa gaaaaaagga gtgacaaacg
     5881 atggatatca taacaaaatt tgctgaggga tttatgaggc tgttccaaac aggtgcagaa
     5941 acattcataa gctggatgac aggaatcgta ccagttgtct tgatgctgat ggttgcaatg
     6001 aatacgctga ttgctttttt aggtgaagac cgagtaacga aagtagccaa attatcagcg
     6061 aaaaatccgc ttactcgtta ccttgtgtta ccgttcttat ctgcttttat gttgggaaat
     6121 cctatgtcat ttacaatggc tcgtttcctt ccagaatact acaaaccaag ctattatgct
     6181 gctcaagctc aattttgtca tacgagcaat ggtgtattcc cccatattaa tcctggagaa
     6241 ttgtttgtat ggatggggat tgctcaaggg atcgaaacct tagggctaaa ttcgatggag
     6301 ctggctatcc gatacttgct tgtgggactg ctgatgaact ttattggtgg ttggataact
     6361 gattttacta ctggatttgt atgcagacag caaggaatcg ttcttagcaa aaaagtagag
     6421 ctttcagttg attagagaaa ggagagtttt aaatgtatgg tgatattaag atcgtaagag
     6481 gaaacggcgg gtatggaggt cctttgatta ttaggccgac aaaagataaa cacaagttca
     6541 tctatgtcac aggtggaggt gagaggccaa tgatcgtaga taaaatagaa gaactgacag
     6601 gtatggagtc tatcaatggg ttcaaaacat caatacctga tagtgaaatc gcgcttgcaa
     6661 tcgttgactg tggagggact cttagatgtg gaatttatcc taaaaaaggg ataccaacca
     6721 tcaatatcgt ttccacagga aaaagtggcc cattagcaca atatatcact gaagatattt
     6781 atgtttcagc agtagggtta gaacaaatac agccactaat ggaaactgat gataacaaag
     6841 aaacagtaaa tactgcatct ccagataaaa agtacaaata tgatacttct aaaaagataa
     6901 ccgagcaaaa agcagagcag caaaactgga tagcaaaaat tggaatgggc gcgggaaaag
     6961 tagttgctac attcaatcaa tcagcaagag aagcagtaga tacgatgatc cggacagtga
     7021 tcccttttat ggctttcgtt tcccttttga ttggtgtgat acaaggttct gggatcggta
     7081 atgtatttgc taacattatg gcacctttag caggaaatgc agttggatta acaatcattg
     7141 gctttatttg ttcactgcca tttttatctc ctttgcttgg tccaggggca gttattagcc
     7201 aagtgattgg aacattgatc ggtgtagaga ttgggaaagg aaatattcct ccccaattag
     7261 cattaccggc actatttgct atcaatactc aaaatgcatg tgattttatt ccagtcggtc
     7321 taggtttgga agaagcaaaa gctgagacag tagaagtagg tgtaccatca gttctttatt
     7381 caagatttct taacggcatc ccacgagtta tcattgcttg ggtagcaagt tttggattat
     7441 atagctgagt ttagtctaat ggtgataagt caagatgaaa tttaagattt tctttattga
     7501 gtaatatgtt ttgaagcgca cgaaactaga cccgatcaaa atcaaatttt tttcgggttg
     7561 attcaaggat ttgcgtcaat aacggattgt tatttctcta tcacactcct tggtggcgta
     7621 tagagttggt gtttacgtag tagcgtatcc accagacgcg taaattttct tgcggttagg
     7681 acgagtgcac gtttgtgttg atgctttgga acctccttat atttactttg ataaaaagct
     7741 ttgtattcag gaagataatt ttttacagag ttggcagctt caactaagta gtatctaaaa
     7801 tatcgatttc cttgttttgt cagaggtgta ttttgcgact gatagtttcc agattggttc
     7861 actttccaac ttaatccagc gtattttgcc aattttgtct gatcttcaaa tctttcgatt
     7921 tgaccaattt cagcgattaa accggcagca tagactttac caacgcctgg aatactggtt
     7981 aagcattgat attctggaat gacaacaatt acttgttcga tggctttatc taattctttg
     8041 atattttttt caagtgctcg tatttctcgt acgagaacac ttaaaacaac attaattgat
     8101 tcttgtgcaa gagcacctag gcggtaactc atggtaattg cacgttgaat tgctttagcg
     8161 attttttctg gttgcttgaa acggcctttt cctttttctt gaagtaaatc acagaaagct
     8221 tctaaaggaa gctcagcaag ttcatccaat gtgaaatctt ccgtcatgag tgaaatgagg
     8281 gttgcactaa aaagactcgt cgattcgtct cgcatttctc gagcaagcgt attacatttg
     8341 tacgttagat tttcaagaaa atgttgcttt gtacgaatca attgcttaat aagttgatag
     8401 cgagtacgtg ttaaacgctg taaggccatg tatttctctt ctttgatagg ggaagttgtg
     8461 aatcgttgaa tacgcaagaa atcagcgatt cttaatgcat cgtttcgatc agttttattc
     8521 tcatcaaaca tgcgactaaa ttgtttcacc cgaaaaggat tttcaatcgt tacaagtgtg
     8581 ttgagcttct ttagtttctc atcttcatga aaaaacatgg aaggatggaa gctgtacatg
     8641 gacgttgact ccatgccaat cacaa
//
LOCUS       DAZKLA010000114         8614 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000114 DAZKLA010000000
VERSION     DAZKLA010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.116"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(212..2593)
                     /locus_tag="ACG85N_002308"
     CDS             complement(212..2593)
                     /locus_tag="ACG85N_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0225030.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2583..3230)
                     /gene="recU"
                     /locus_tag="ACG85N_002309"
     CDS             complement(2583..3230)
                     /gene="recU"
                     /locus_tag="ACG85N_002309"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFQ0225031.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3294..3833
                     /locus_tag="ACG85N_002310"
     CDS             3294..3833
                     /locus_tag="ACG85N_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFQ0225032.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3923..4342
                     /gene="gpsB"
                     /locus_tag="ACG85N_002311"
     CDS             3923..4342
                     /gene="gpsB"
                     /locus_tag="ACG85N_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFQ0225033.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4364..4731
                     /gene="rnpB"
                     /locus_tag="ACG85N_002312"
     ncRNA           4364..4731
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG85N_002312"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4940..6106
                     /locus_tag="ACG85N_002313"
     CDS             4940..6106
                     /locus_tag="ACG85N_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFQ0225034.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6152..7648
                     /locus_tag="ACG85N_002314"
     CDS             6152..7648
                     /locus_tag="ACG85N_002314"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFQ0225035.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7729..8412
                     /locus_tag="ACG85N_002315"
     CDS             7729..8412
                     /locus_tag="ACG85N_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0225036.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8421..>8614
                     /locus_tag="ACG85N_002316"
     CDS             8421..>8614
                     /locus_tag="ACG85N_002316"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225037.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2706 a         1566 c         1830 g         2512 t
ORIGIN      
        1 attataaaca agtaaatgga taaagtatat gtaatgataa aagaaaaatg gattttctaa
       61 tttttaagct aaatgttaga tcacacatgt atatgctgga tagtcttata aaaaatcatg
      121 taatacagct tggtacatga tttttatttg tagctgtttt ttacatagac acaaaaaaga
      181 aaccacgctt gtagacgtgg tttctttttt actatctgtt tccagaagct gaggtgctgc
      241 tggatggtgt atggtcattt gcagcatccg ctccgctagt tgctggctca ctggaagaag
      301 caggaggagc agatgaagac ggtgtcgagc tttcttcgga tgaagaagaa ggaaccgttg
      361 ttgaagacgc tggctcgctt gtttctgctg tcgtgctttc ttttgaggac tcagcagttg
      421 attcgctttg actagtagag ctagagcttt gagtcgttgt ctctgttgtt gagcttccag
      481 gagtctggac atagctggaa gatggaatag ttgttgatgg tgtaattgca ttcgatctag
      541 ctgtatattg atctttgtag tacagttcac cgcccacacg aattaatccg cttggcatct
      601 cccagtctgt atttgtcacg ttagctgaaa catactgcat aagctcgcga taaacatcag
      661 aggctacgtg tgacgattct gaagtgacag gcgtcatttt tttattgtaa cctgtccaca
      721 cggaaatcga ataatttggc gtatagcccg caaataggat atctggatac accccagaag
      781 aaataccaag ttttgcatac tcgtcatctg tgtaattgga tgttccggtc ttaccggctt
      841 gatataaccc agcgatttgc gcattcgtac cagttccttc agtgatcgta tcttttaaaa
      901 tgtctgtgat catataagct gtttctggag acatggctgt ttttccatct ggctcataag
      961 tttcttctgt tccgtcttgg aagacgattt tattaacata ttgaggtttg taataagtcc
     1021 ctccattggc gaaagccgca taggctgctg ccatttttaa ggaagaagct ccgtattttg
     1081 tcccatcttg ttcttccgta ttactagaga tcgcattcga ttgatggatc gtactgtatt
     1141 cgatcccaag attttttaag aaagaagcca ctttatctga accgacttca ttgaataatt
     1201 tgactgctgg aacattacgg gataagtaaa gtgcttggcg taaagtaatg gtgcccatat
     1261 attgattatc ccagtttcct acaggagtcg aagtcccttc atagttatac ggtgcgtcag
     1321 taatagtttt tccagtagaa tatttcaaat attcaaaagc tggtccgtaa tctgtgacag
     1381 gtttcatcgt agaaccaaaa tcacgggaag tattcactgc taagttgttg cctaaagtta
     1441 cgtcttctgc gatgtgacgt ccgccaatct gggcttttac tttcccggta ttggtgtcga
     1501 taagggtgga ggcaacttgc atctcgtcgt ctgggtaaga gacatactga tccgtgttga
     1561 cgatatcata tagtttcttt tgggcgtcca gatccaaatt cgtataaatc tctaaaccgt
     1621 ctgtgtagac attcttatct gttttttctt gtacttcatt gatgacttct ttgacataat
     1681 tatcgacgat cttagtattg tcatcggatt gagttagttc ttgcagacca tccgttaccg
     1741 gtacattaac agcttgatca tattctgttt gagagatttt ttcgttttga agcatcgtat
     1801 acaaaacagt atctcgtctt ttcttcgcct gatctggata aacataagga tcataggcgg
     1861 aaggggcttg aggcatccca gcaagcaatg ctgtttgcgg taaagaaagt tctgataatt
     1921 tcttaccaaa gtacatctca gaagctgtct ccatcccgta caagccgttt gacatataca
     1981 ctttatttac gtagtaggtc aagatctctt gtttcgattt tttttgttcc aatcttacag
     2041 ccatccacgc ttcttgggcc ttgcgtttca gagtctgatc ctcggcactg gtggagaaga
     2101 aggaaagttt gatcaattgc tgtgtcaaag tacttccgcc ttgcagtccg ccagaggtaa
     2161 agttagagag ggcagaaccg atgatccgaa tagggtcaac cccgatgtgt ttataaaaac
     2221 ggcgatcttc taccgacacg atcgcatctt ctaatgtttt cggtaattca ttcgcagaaa
     2281 ttttttcacg cttttctgcg cctaaatctt cgaatagttc gccatcttgt gtatatagtt
     2341 tggaagatac ggtagcgtct aattttttat ccgtcaattc gggcgcatct tttgcgtaat
     2401 accagaaaag tcctacacct gctaaaaaga ggatacagaa aaatgagagt attcctaaaa
     2461 ggatcttgat aaataatcct tttttatgtg ttccagagga ctttccagaa cctttgccag
     2521 aattcttctt cacactcttt ttaggagtag gctgtttctg tcgtctagag cttctagttt
     2581 gttcatttgc cattcttgct ttctccttca atagataatg ttgataaata gaagtcaacc
     2641 gcgtccaaat aaggaatccg cggagaaata ccagttttga tctctattcc tagttcttcg
     2701 atcaaagata aaggtagtga tttgcgtcct ttttcctttt gggcgtccca gtaagcaatc
     2761 aaatgaacac tattcaaata aaaacagcgc ttcaaagacg aaaaccataa caagacaaag
     2821 cagaccccat tttgttcgag gcatgctttc atatgttcga tttgatgggc atggaagttt
     2881 ttaaagggaa aagatgtttt gttcttggtt tcttttgctt caaagtccaa gtaaaagcct
     2941 cgatagacgc cattatagtc tgtagttgag gcttgggtaa agtaggcttc cttgatcaca
     3001 gcagcacttc tcttaggata gtctactttc acgatttgga taggggtagg ctttttatgg
     3061 ataaccgcca acccgcggga aagataatat ttattacttt cattgattgc ttcttcaaag
     3121 cgcatccccc gattgccgaa ttcagtgatc tgatttcttt tttcttgaat tttttcgacg
     3181 ggagaggcat tcttataata aggttggcca tttggatagc gcaataccat ctttaatcac
     3241 actcctaggc cacattatag caaaaaagcg gatagaaaga aaaggaatct gagatggata
     3301 agtcaaaagt aatctatgtg tctgggtatc gaagtttcga actcggtgtt tttaaagaaa
     3361 atgatcctaa aatccaagtt gttaaaaatg ttttaaaaaa agaactccat cagttagcag
     3421 aagaagggct ggaatgggta cttgtcagtg ggaatttggg cgtcgaacta tgggctgtgc
     3481 aaatagtagc ggaattgaaa gaagaatatc cggaactatc tcttggtatt ttgtatcctt
     3541 tttctgattt tggtaataac tggaatgaag ggaaccaaga aaaaaaacaa ttagcagaac
     3601 aattagcaga ttatgtcgaa gctgttagcc atcaacccta ccaatcacct agtcagctaa
     3661 agaatcacac tcgttttata cttgagcata cagtgggctg tctgctgatc tatgatgaag
     3721 aattccctgg aaaaacgaag tatttttttg aagatgcaca gaattaccaa gcagatcatc
     3781 cctacgaaat tcggctgatc agtatggacg atcttcaaaa ttttagtgaa taatcgatat
     3841 tgtgtttgat ttatcaactt ttttcctata caatgaaaaa ggtagaaatg ttattacgta
     3901 aatgaaaact agaggtgtaa atatggcgaa tttggtttat agtcctaaag acattttaca
     3961 acaagaattc aaaacaaaaa tgcgcggcta tgatcccgtt gaagttgatg aattcttaga
     4021 caatatcatc aaagattatg aaacttacag caaagaactc cttgctttac aagaagaaaa
     4081 cgatcgattg agtgcaaaag tagctcaatt atctaaaact caaggagcag ctcaaacacg
     4141 cgtgcaacaa acagaggcac caaagagcgc agcagtgacc aattttgata tcttaaaacg
     4201 cttgtcaaat cttgaacgtg aagtatttgg taaaaagctt gatcaacaag caagtgcagt
     4261 caaaccagca caacctaatc caaataacta cacaaatgca gacacaagct tagatgataa
     4321 tgaaaaaaca cgtcaatttt aatttttgat taatgaattt gcggttttcg ggtaatcgcg
     4381 gtctgacttg atcaggctga ggaaagtcca tgctcgcaca ggctgtgatg cctgtagtgt
     4441 tcgtgcttag cgaaaccata agctaaggta ctctttggag taacggcagg aaaacaagct
     4501 aaggcttcgg ctatgcttaa gtatccttga aagtgccaca gtgacgaagc ggctggggaa
     4561 acccatccgg tggaacgcgg taaacccctc gagcgagcaa cccaaacaat ggtaggggcg
     4621 ctttttgtct ggaagaagaa caggacaaaa aggcagtata actgcagata gatggttacc
     4681 gctttgtttt ttctccctgg aaaagcaaag tacagaacat ggcttataga aaaccgcaaa
     4741 catcagggaa ttaggtcgtg acaaaagtaa tgcgttataa gaaacaagaa aaacatttga
     4801 aagctatttt tcaatacttc agatgaatcg ttcttattac aaaacaaact tttgtatcac
     4861 cggttattcg gaatttgagt aaaagtgtgg ggtaggactg atgtcccacc tccttttttt
     4921 ataaataagg agattgtgta tggaaaaaaa caggaagttc aacctcgtcg caactgcggc
     4981 aagcgggcta gaagcactgg ttggtaaaga attaagagat cttggaatcg aatgccaagt
     5041 tgagaacgga cgtgcacgtt ttgaaggaac aatggaaacg atcgctacag ctaatttatg
     5101 gttacgtaca gcagatcgag taaaaatcgt ggttggggaa ttcgatgcgt atacgtttga
     5161 tgaattgttt gaaaacgtaa aagcattgcc ttgggaagat tttcttccac tagatgcagc
     5221 ttttcctgta gctggaaaat cgatcaagtc gaaactatat agcacacctg attgccaagc
     5281 gatcacaaaa aaagcaatcg ttgaacgtct ccgtacctat tatcaccgtc cagcttctgt
     5341 accactgacg gaaactggcg cacattttca gttggaagta gctttgctga aagaccatgt
     5401 gatggtaact ctggatacga caggaccaag tttattcaaa cgtggttatc gcttagaaaa
     5461 aggtggagca ccattgaaag aaaacatggc agcagcactt attatgttga caaattggcg
     5521 aaaagaccgt ccattttatg atcctgtatg tggttcaggt acgctttgta ttgaagcagc
     5581 cttaataggc cataatatcg caccaggatt caatcgtgag tttgcttgcg aatcttggga
     5641 ttggttcagt caagaaatca tggaaaatgt tcgatcagca gccgaagaaa aagcagatta
     5701 tgatattgaa ttagatatta ctggttcaga tatcaatgga cggatgattg aaattgccaa
     5761 agccaatgca gaagaaatcg gattaggctc ttcgattacc tttaagcaac aagcagtcaa
     5821 agacttcaaa acggataaag aatacggggt catcgtggct aatccgcctt acggagaacg
     5881 tctaggagaa gaagaaactg ttcggaaatt atataaagaa atgggcgatg tattccgtcc
     5941 attaaaaaca tggagtaaat atatcttgac tagcgactta gcgtttgagg aattttacgg
     6001 acaaaaagca accaaaaaaa gaaaattgta caatggtgca ttgcgtacgg atctattcca
     6061 gtattgggga actcgtccgc ctagaaaaaa agagaaagac gaataatctt actaattgat
     6121 tcattcaatt ggatgtttgg aggaataggg aatgaaagaa aaagagtttt tacaagaagt
     6181 aaaagaaatc aatcttctac aacaagcttt aggaatcctc gattgggata cacaaacagg
     6241 tatgcctgaa aaagctagtg cataccgaag tgaagtagac agctatcttt acagcattta
     6301 tttttcaaaa aaaatcggtc caaaaatcaa agaagccatt cgctattttt ctgaacatcc
     6361 ggaagaattg tcggaagttg gaactgccgt ttttgccaaa gtaaaagaag agtatgattt
     6421 ggaacatcgc gtaccggaag atcttatgag aaaatataca gctgccgttt cttcagcaca
     6481 cggacaatgg caaaaagcta gagaaacaca acaattttct gattttcaac acgcattgaa
     6541 tcaaaatatt gaattaacaa aacagttgat accttactgg agaaaagatg aaaagacgaa
     6601 ttatgatgtt ttgctgaatc aatacgaacc cgggatgact gtcgaaatcc ttgataaggt
     6661 atttgatcag cttaaagaag gaattctttc gatccgccgt acgctccaag aaaaagggac
     6721 tgaacctgaa acagactttt taaatcggag aatgacgaaa aaacaacaga agagatttgt
     6781 agtgaaagta atcgaacaac ttggttatga ttttagtcgt ggtcgtttag atgacactgt
     6841 ccacccattt atgactggaa ttaatccgaa tgatgcacga atcacgacac gatggaacga
     6901 aacagatttc aaaatggctg tttttggagt gatccacgag gctggtcatg gtatgtatga
     6961 acaagacatc gatgataaat atgcgtatac accggtttat gaaggtacat caatgggtat
     7021 ccatgaatca caatcgttgt ttaacgagat cattattgga agtagtcgca gcttctggaa
     7081 aaagcaatat cctttctttc aagaatgtgc agaaggaaca ttcgatgata ttccttttga
     7141 caaattttat cgttcattga accatacaaa atcaagtttg gttcgtatcg aggcagacag
     7201 tttgacttac cctctccata tcatcattcg ttatgaaatc gaaaaaatga ttttcaatga
     7261 aggggtagat gttgctgaac tacctgaaat atggaatcaa aaatatgaag agtatctggg
     7321 aatacgacca acaaatgatg tggaaggcat cctacaagac gttcattggt caggtgcaag
     7381 ctttggttat tttccctctt atgcattagg gtacatgtat gcggcacaat tacttcattc
     7441 tttgcagcaa gaaatagatg tggaaaatgt tctagcgtca gacgattatt ctccaatcaa
     7501 aaactggatg tcagagcata tccatcaata tggtgcatct agaaaaccaa atcaattaat
     7561 catggatgca acacatgaac cgcttaatcc gcaatacctg atcgactata tgaaagacct
     7621 gtattttaat gtatatcagg taaaataaaa tacaatttac aaagatagct gaaattcatc
     7681 taatctatag atgttttcag ctatttactt aagaaagaag tggaacgaat ggataaatta
     7741 caagtgatcg ctgattatag tatggaccaa ttagcgcatg atcaaacagg acatgggagc
     7801 gatcatacca aaagagttgt caagttagct gaaaaaattt tggagacgga accgcaggca
     7861 gaccgctttg tgacgcttgc agcagcttac ctacatgata cgattgatga taaagtcgtt
     7921 aaaaatgaga acgaggcaaa gcaacagctt cgtgtattcc ttcgctcttt gccaatagta
     7981 gaggaacaga tctcgatgat tttttccatt atcgaaaata tgtccttttc taaaaatctc
     8041 tctgaagcgg ccgaactatc tctggaaggt aagatcgttc aagacgctga tcgtatcgaa
     8101 gcattaggag caatcggcat tttgcgtact gcttatttcg gcggtggaca tgggcacccg
     8161 atttttgatc ctgaactgta tccgcaaaca ttcaaggaca aagaaaatta ccgaaaaggc
     8221 acgacagtga tcaatcattt ttatgaaaaa ctgttcttgc tgtctgataa aatgaatact
     8281 gattacggat atgaagaagc caaacgaaga gaaaccttca tgcgtgaatt cttagaagaa
     8341 ttttttctgg aatggtctat tgacgatgaa aattttactc cagacaattg gatcaaaaat
     8401 aacaaaaaat gatggaggtt gtgacagaag tggtcagctc caagatattt cggatcattt
     8461 tccgtaaaag ttacttctgg aacactgttt accaagaggt cgtgagcctg ccgttcagct
     8521 ccgagtcaca aggaacaatt tcaaaaaata gcttctcgtg tttgatgaat attgtaactt
     8581 gtgattcgga attggcagga gaacaccgtt tatt
//
LOCUS       DAZKLA010000115         8612 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000115 DAZKLA010000000
VERSION     DAZKLA010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.117"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..561
                     /locus_tag="ACG85N_002317"
     CDS             52..561
                     /locus_tag="ACG85N_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0225038.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            654..1816
                     /locus_tag="ACG85N_002318"
     CDS             join(654..930,930..1816)
                     /locus_tag="ACG85N_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0225039.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2096..3076
                     /locus_tag="ACG85N_002319"
     CDS             2096..3076
                     /locus_tag="ACG85N_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0225040.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3198..4616
                     /locus_tag="ACG85N_002320"
     CDS             3198..4616
                     /locus_tag="ACG85N_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0225041.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4687..6156
                     /locus_tag="ACG85N_002321"
     CDS             4687..6156
                     /locus_tag="ACG85N_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0225042.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6166..6633
                     /locus_tag="ACG85N_002322"
     CDS             6166..6633
                     /locus_tag="ACG85N_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0225043.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6669..7541
                     /gene="scrK"
                     /locus_tag="ACG85N_002323"
     CDS             6669..7541
                     /gene="scrK"
                     /locus_tag="ACG85N_002323"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFQ0225044.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7623..>8612
                     /locus_tag="ACG85N_002324"
     CDS             7623..>8612
                     /locus_tag="ACG85N_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0225045.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDS"
BASE COUNT         2967 a         1294 c         1750 g         2601 t
ORIGIN      
        1 gtatagccct aaacaaatta tttgtgtttt attgtttagg gctatactat attggtacaa
       61 ggaatagaaa gaggtgttta tgtggttgat aaaattcagt ttaatcaagt tatagaagaa
      121 ttcatcaaag taaataattt aatgacccat attcaaaaac gaccaatcag aataactaat
      181 gatctaaaac tcagtacgag tatgattcat ttgattgatg tgattggaaa ttatcctgaa
      241 actagtatta cggaattagc tgatcgatta ggcgttacca aaggagctgt atcccaacag
      301 attcccactt tagagaaaat gaatcttata agcatttctc aaaaaaaagg aaataagaaa
      361 actaaacttg tatcgttaac aaaatatggt cgaaaggtgt acgaatcaca taattattta
      421 catgaagagc tttatagctc aataaatgaa gcgttagaat cattctcacc agaccaaatt
      481 tcaaccatta aacagattct agaaaaagta tcttatacaa tcaatgaata tcaagtaagt
      541 ctgacaagaa aggaagattg acatgaaaaa aatgattttt gaatcggcta cactagacta
      601 gacagaaaaa ataaggtgtg taaaataaat gaaaacacac tggaggaatt tttatgtcaa
      661 gacgtcaacg tagaacctat tcaaaagaat tcaaacaaca agtcgtcgat ctttatctcg
      721 caggaaagcc ccgagctgaa atcattcgag attatgagct gactacctcg gcctttgata
      781 agtgggtcaa gcaagcgcaa actactggct cgtttcagga aaaagataac ttgacccctg
      841 aacaagtaga actaattgca ttacgaaaga aaaataaaca gcttgaaatg gagaatgaca
      901 ttttaaagca agcggcgctg atattgggac gaaaagacaa gtaattgata ccaataagca
      961 caaatactca atatcagcga tgtgtaaagt gttaaacatc tcttgccaaa cctattatta
     1021 tcaagccaaa ccagttgaag atgaatcaga actggaagaa ctcgttcaag aagaatttat
     1081 tcgcaatcga aaagcttatg gtaccaggaa gctgaagcag tgcttggcca atctaggtct
     1141 tcaagtgagt caacgcagaa tcggccgtat gatgaaacag cgtggcttgc gttcaacata
     1201 cacattggct catttcaaat tccattcttc tacttgtaac gaagccaaga ccgccaatat
     1261 tctagacgga accttcctac aggaaaagtc attagaagcg attgtaaccg accttaccta
     1321 tgttcgtgtt gggaagaaat ggcagtatat ctgcctgatt cttgatttgt ttaatcgaga
     1381 aatcatcggc tattcatgcg gggagaagaa agatgcgacg ttagtcaagg aagcttttgc
     1441 aaagattccg tactcgttga ataatgtaca actttttcac acggatcgtg gaaaagaatt
     1501 cgataaccag acgattgacg aaattttatg ggcctttaat atcacgcgtt ccttaagcaa
     1561 aaaaggctgc ccctatgata acgccgttgc agaatccacc tataaatctg tcaaagtaga
     1621 attcgttcat caataccgct ttgagacatt ggctaatttg cgattagagc tatttgatta
     1681 cgtccattgg tggaatacca tccggctgca cggcacgtta ggctatgaga caccgatcag
     1741 ttttcgacag cagagattgg cgcagcgaat ccttgataat gagctcggat gtgataccgg
     1801 tggcgaggca ccttaattat ttgaatgagc ttctgcctta gagaatcaga ttcgaggagg
     1861 ctcgttgtca agggcagtcg gaacaccagc agcaagctta cgcaatacct tataattttt
     1921 gtcaaaaaaa gtgttgccat tccacatata tctttaaaca gctattgcca gataaaaata
     1981 aatgatatta tatatataaa tacttatccg aaaaggggaa tgagagagtc aaggttcttg
     2041 gaatgtcgaa tagtcgagta ggtagatttt ctatctacaa ccttgttgtt taaaaatgaa
     2101 aaaaattgga attagagaag ttgcgaaaga ggcaaatgtt tctcctacga ctgtgtcgag
     2161 ggtattgaat gatcgtggat atttgagtga ggaaacaaaa agaaaagttt ttgatgccat
     2221 gaaaaaatta gaatactatc ctaatgaact ggcgagagca ctttttaaaa acgaaacata
     2281 taccgtaggt ttgatttttc ctacaattac taatccattt cacgctgagt taattcaaga
     2341 tatcgagttc gagctttcca gcaggggata taaagtgttg ctgtgcaata gcttaaacaa
     2401 tccaacaaaa gaaaaagagt acttgacgat gttgagaaag aatcaggttg atggaattat
     2461 agtaggaacg cacaataaag atattgcgga ttatgatatt ccagggctac ctattgtagc
     2521 gattgataga agtcttggtg aaaatacagt tacagtatct tgtgataatt acaggggtgg
     2581 ggaattagct actcaactac ttgtaaatca tggctgtcag aatatactgt gtattcgggg
     2641 agatagtaag ataaagctac ccgcgaatag taggacttta gcatataaag atgttttgaa
     2701 taaattcatg ctggaaccgt tagtcctaga agtaccattt gtaaaagaaa tcaatgaaaa
     2761 aaatgaaata attaagacat atctggaaac tcatccaaat attgacgggg tttttgcagg
     2821 agatgattta ttggcttctt tggcgattaa caacttagga agttcgggca aaagagttcc
     2881 aaaagaggtt aaagtgattg gatttgatgg agctcaacaa acattgattt ataatccaga
     2941 attatcaact attcaacagc ctatagatca aatatctaaa gtagcagtaa aaaaattatt
     3001 gaatatgatt aagggcgaaa aggagacgga tgaactttca cttccagtaa agctcataaa
     3061 aaaaaataca gcataagtta aatcggcgaa aaattccttc gtcgatttaa aaatattttt
     3121 atgaaacggg ttgacatatg aaacgggttg acatatatta gttgtgaaga taaaaacctt
     3181 ttcactggag gaagaaaatg gagaaattat taattaaaag caataatcat caactttact
     3241 accaacctaa ggatgtttgg gtcggagatg ttatgcctta cgggaaagaa ggaaaattct
     3301 ttatttatca tcaacgtgat actagaaatc cagttccttt tggagaaccg tttggctggg
     3361 ctttagcaac tacgaaagat tttgttcatt ttgaagactt tggggaatct cttttaagag
     3421 gggatgacaa tgagagagac cagtttatct acgctggttc tgtatttgag gcaaatggaa
     3481 aggttcacgc attttataca gggtataatc gtgaattctt agcgcaggga aagacatctc
     3541 aagtgttgct tcatgctgag agtgatgatt ttgttcattg ggaaaaatca gaaacagcat
     3601 tggagttaga accacaagaa gggtatgata ttcgaaactg gagagatcca tttgtcgttt
     3661 gggatgatga aaaagaagaa tatttgctaa ttttaggggc gcgaaagggt caggataaac
     3721 atctacaaac cggacgttta gtggaattta catcaaaaga tttaactaat tgggaattca
     3781 aaggtgattt ttgggctccg ggattgtata ccatgtttga aatgccagaa cttttcaaga
     3841 ttggtgactg gtggtatctt gtctattcag aatactcaga tggaaataaa atactctatc
     3901 gaatgagtca aagtttggaa ggtccttgga ttaagcctaa agatgaagct tttgatggac
     3961 gggcttatta tgcaggtaga acagcttatg atggaaaacg tcgagtccta tttggatggg
     4021 taccaacaaa agatcaagaa aatgataaaa acaattattt atggggaggt actttggttc
     4081 ctcatgaagt ctatcaaaaa gaggattttt cacttggggt aaaacccata gacgaagtgt
     4141 ggaatgcatt cataaattta catgatattg ccgatgtaga gatagaaaca gcagacacaa
     4201 gagaagaagt aacgcttgtc aaaaatagtg gaactttgtt taatttagaa acaacaattt
     4261 catttgatga gaatcttagt gacttttcac tgagattata taaaaacctt gagacagatg
     4321 aatcttacga atatcgcttc aatttagatg aggggcgtct tacatttgat aaaaaccctt
     4381 gttacccttg gtttcaatat atggataaag gacttattag acctatatct ttaaagccta
     4441 accaatctta taaactaaaa gtgattgttg atgacaatat aatgacactt tatattgatg
     4501 gaatcgctct taacgctcgt gtacatggtt gttttggtca agcaattact attacagcaa
     4561 caaatggatc aatgactgca ataaatacca agttttcaaa tgaaataaag aaataataaa
     4621 cgtcaatcat gtggcaggtc tgaaaatcaa acatgacctg tcacatttaa aagggaggat
     4681 aaatgaatgg actacaaaaa aattgccgaa gatgtattgg aaaacgttgg aggaaaagag
     4741 aatatctcaa gcgctacaca ctgcattact cgtttacgtt taattcttaa agacaaaagc
     4801 aaagcgaata tggaaatgct tgaggaaata gaaggtgcaa aaggtgttct tttcaatagt
     4861 ggacagttac aagtaatttt cggaacaggt ataattgaaa aggtttacaa agagttcgtt
     4921 gaattgacag gagctaagga agtatccgtt tctgaaatga aagacgaagg agtcaataat
     4981 tcaggaaata gacttcaacg agcttttaaa gttttctcgg atatctttat tccgatcatt
     5041 ccagctttta ttggggcagc aatggtttta ggaattcgtt cgttgattac cactcctgga
     5101 atgtttggaa tggatggctc attcgctgat cacaatgtat tcctaaatga tttatccaaa
     5161 tttctgggta ttattgcaac cacatttgat tatttaccag ttttagttat gtattctgca
     5221 acaaaacgtt ttggcggaaa ccctatttta ggattacttg taggatttgt tatgattcat
     5281 ccaggactag ccaatcggaa tgatttcgtc atgggaacta taaagcctga ttattggcat
     5341 ttatttggat taagcattcc agcagttgcg tttcaagggg gtgtttttcc agcaattttg
     5401 acttcttggt ttttagcaaa agttgagaaa attacaaata aatattctcc tcaagtactt
     5461 gcttacattt tagtcccaac tgtgacaatt gttcttgcca actttgcact atttttagtg
     5521 tttggtccat tggggaattt tatcggtaat gttttaggtg gaatcattga tttcctatat
     5581 atgaagatgg gtgcctttgg tgcttttgtt tttgcaggcg cgttacaacc attagttgta
     5641 acaggaacac aacatgctat tcaaggtatt gaggcaaact tagtcgcaac aactggattc
     5701 aattatatcc aacctatttg gtcagtttca attattgccc aaggtggcgg ttgtattggt
     5761 atgtatttac tctttaagaa aaaatctaaa gatagagata ttgcgatttc aagctttatt
     5821 ccaacactgg taggtattac tgaaccggct atctttgctg ttaatttgaa atattcaatc
     5881 gttccttttg tttgctcgtt tattggtgct ggttttggtg gagcatttat gaaaattttt
     5941 gatgtaaaag ccattggaca aggattgact gtattgccag gtctgactat tgctaatcca
     6001 gttcttcctt atatatttgg taatctcata gcattcattt taccaatcat ttttattctc
     6061 gcttatgata aatataaagt gtttatccct gaagctaaac accgtaaatt tgcagaaaaa
     6121 actgactcaa aaaaattgcc agttcaagta aagtgagatg agagcatgtt aaattttttt
     6181 agaaaaaatg aaataagcgt tgtagcacct gcaaccggtg tttttgaaaa actggagaaa
     6241 ttaagtgatc cagtcttttc aaaaggaatg atgggacaag gatttgcaat tgatgcaaaa
     6301 aacgaaacga ttgtatctcc aatcgatggt tttgtttcaa gtatttttcc tacaaaacat
     6361 gccttaggaa taaaaacaaa atcaggaatt gagatacttg ttcatgtagg aatagataca
     6421 gttgagctaa atggtgaggg atttgatatt aaagttcaag aaggacaaaa agttaaggct
     6481 ggggatattc ttatgaccat taattttgta gtttttgaaa agaatcacaa gactaaagat
     6541 gtcattgttg tttttccaga ctaccaagac gaaattttca ttccgaaact tgaagaagta
     6601 aaacaaggac aagaattgtt tgttcttggc taatgataag taattaataa tagaatcgag
     6661 gaaaatgtat ggtttttggg gctattgaag ctggtggaac aaaatttgtt tgtgccattg
     6721 gagataaaga tttgaacata tttgaaaaag tcagctttcc cacgactaca cccaaagaaa
     6781 caatgaattt agttttagat ttttttaatc atcataaaga tgatttagaa ggtatcggag
     6841 tgggatcgtt cggtcctatt gatattcatt gtgaatctaa aacgtatggc ttcattacat
     6901 caacgccaaa acttgcttgg caggattttg attttattgg aagtttaaaa aagcaactta
     6961 atattccaac tgcttggacg actgatgtaa atgcctcttg ctatggtgaa tatgtttctg
     7021 gaaatggcag aggcctttca agtgttgttt actataccgt aggaacgggc ataggaggtg
     7081 gcgctatcca aaacggaaac ttcattgaag gttttagtca tccagaaatg ggacatatgc
     7141 ttgtaaaacg acacaataaa gacaactttt ctgggacatg tccattccac ggagactgtt
     7201 tagagggctt agctgctggt ccggcaatcg aaagaagatc agggaaaaag ggacaagaag
     7261 tgactgaaaa tgacgttact tgggaaatag aagcttatta cattgctcag tgtgcatata
     7321 atacaacatt gatgttatca cctgatgtca ttatatttgg tggcggagta atgaaacaaa
     7381 aacacttaaa aaagaaagta caagataagt ttattgagtt gatcaacgga tatgtcgcta
     7441 ttcccgatgt tgacaattat attatgacac caaaattgtt agataattct ggtattgtag
     7501 gttgtttagc tttagcaaga gacgttaaag aaaaatctta atttggaaaa aagatcgtct
     7561 cgactatttg tttggttgat gcgatctttt ttttcaatta gtaattgaag ggacgcctaa
     7621 aagtgaaaaa aaaaatgaaa gatgaagtat tagagtatat caaaaataat ttaaagtatt
     7681 acgatttttc tgctcaagat attgcaatga aattttgtat taaaagaaat gtcgcttccc
     7741 actatttgaa tcagttattt tcagatggaa aattactaaa gaatgattct gttagaccag
     7801 taatgttcaa gtataatcaa caaaagccaa aagactgctt ttctaagttt attggagcag
     7861 acatatcttt gaaatccact atagataaat gcaaagctac tgtaatgtat ccccctaatg
     7921 gcctcccatt aataataaaa ggaaacagtg gcgttggaaa aagtttttta gcaagtttga
     7981 tctatcagta tgctcttgat agaaaagtaa ttcataatga cgcaaaattt gttgttgtga
     8041 attgtgcaga ttatgcaaat aatccagagc tattgtcagc agttttattt ggatataaaa
     8101 aaggagcctt tactggggct gaaaaagata ctgcagggat gctatcttct gctaatggag
     8161 gatacctttt tttagatgag gttcacaatc taagtgcaga aaaccaagag aaattgtttc
     8221 ttcttatgga ttctcaaaag tatcgtaaac ttggtgaaag tgtgcaatgg gagtatgcaa
     8281 atgttcgcct tatcttagcc acaacagaag ataaaaatac ctctttgtta gcaactttta
     8341 gaagacgcat cccagctgaa atcactctgc cagattacaa taatagaagt acatctgaaa
     8401 aaattcaatt attatttgaa tttcttaaag atgaagcaat tacaattgat tcaaaaatat
     8461 tttgttcgat tgaacttttt actgatcttt tatcgaaaac atttgaagga aatgttggag
     8521 aattgaggaa tgaaatcaag cttctttgtg cagaaggtta tttaggaaat aagagaaagt
     8581 cttcaattta tttaggggac aaactagaca gc
//
LOCUS       DAZKLA010000116         8536 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000116 DAZKLA010000000
VERSION     DAZKLA010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.118"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            69..356
                     /locus_tag="ACG85N_002325"
     CDS             69..356
                     /locus_tag="ACG85N_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225046.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            772..2310
                     /locus_tag="ACG85N_002326"
     CDS             772..2310
                     /locus_tag="ACG85N_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFQ0225047.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            2307..3272
                     /locus_tag="ACG85N_002327"
     CDS             2307..3272
                     /locus_tag="ACG85N_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0225048.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3259..4581
                     /locus_tag="ACG85N_002328"
     CDS             3259..4581
                     /locus_tag="ACG85N_002328"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0225049.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4644..4748
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4822..5565
                     /locus_tag="ACG85N_002329"
     CDS             4822..5565
                     /locus_tag="ACG85N_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0225050.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5580..6572
                     /locus_tag="ACG85N_002330"
     CDS             5580..6572
                     /locus_tag="ACG85N_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0225051.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6584..7321
                     /locus_tag="ACG85N_002331"
     CDS             6584..7321
                     /locus_tag="ACG85N_002331"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0225052.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            7311..8033
                     /locus_tag="ACG85N_002332"
     CDS             7311..8033
                     /locus_tag="ACG85N_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0225053.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8117..>8536)
                     /locus_tag="ACG85N_002333"
     CDS             complement(8117..>8536)
                     /locus_tag="ACG85N_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0225054.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2760 a         1464 c         1839 g         2473 t
ORIGIN      
        1 tcaagaggaa aatgaaaaaa aattattttt ggctgtttct ttttatttat caaataagga
       61 gtggaaaaat gcgagcagca gacttattga caattcttac aaaatcaccc aaaaaaacag
      121 agtatcaagg agtttttttg gctcaagaac aaaaactggt gcctgtgaca atggtccaga
      181 taaataaagc aaatcaattt atctttttat ttgaacacgg caaagcacca cttgctatga
      241 aagacatctt tctttttttg atgaagaacc gtgagaaagc tgtttattat cagtcaaaag
      301 aagaactcat tcctctttat ggagtgaaag agatagaaaa taaagtggtg atataagtaa
      361 aaataagaaa acaagacgtt tatgatgtca aaacaaccat tttttacatt tttggcaaaa
      421 aagtgtcatg atacagtata atattcgttg aacaggccaa taaaggtgtt ctcccccacg
      481 agatttctac tttattggct gtttcttttt attaaaataa aagttgtgtg tgtaaaaaag
      541 atatagctga ttccaagtga caatttcgta tagattttta tggtattcgt agtgaaaaaa
      601 cgagaaccaa aaagttgatc cgactttgtt gcttggattc tttttttgtc agcatattta
      661 gaggtatttt tttctttttt ttgttcgtgc tgtaaggtta aggcaagaca agttagttgg
      721 gaaaaaggaa agaaatgttt atacttaaag aataacagaa aggaaggtcg catgaataat
      781 tttatacaaa cattaagtga aagaaaaggc gagttgctgg aagctgcatt tcaacatctg
      841 tccatttctc tcatttcttt gctgattgca gcactgattg ctattccttt agcgatttgg
      901 gcagccaatc ataaaaaaat ggcagaagtc ttattgcaga tcacgggtgt gctccaaacg
      961 attccttcac ttgccttatt aggtttactt atcccatttg tagggatcgg aactgttcca
     1021 gcattgattg cacttgttat ttacgctttg cttccgattt tccaaaacac gtatattggt
     1081 ctagctgaaa ttgacccgtc tattgaagaa gcagcggtag catttggtat gtccagaatg
     1141 agacgcttgc tgaaagtaga gcttcccatc gcattacctg tcattatctc aggtatccga
     1201 actgccttag ttttaatcat cggtacggct actttggcag cattgatcgg agcaggtgga
     1261 ttagggacat ttattttgtt aggtattgac cgaaatacac ctgttttgac attgattggt
     1321 gcgatcagtt ctgccttgct tgcaattata ttcagcggac tgattcgctg gttgcagcat
     1381 aagaaacctc gatattccat cattacttta gcagcagtgg tgtgtttatt tggcggcgtt
     1441 tctttatatc agtctgcctt gtttaaaaat gaaaccatca cgattgctgg aaaattagga
     1501 gcggaacctg aaatcctgat ccaaatgtat aaagaattga ttgaagacaa cacaaaaaca
     1561 gaagttgaat taaaaccgaa ttttggcaag acgagttttt tgttcaatgc actggatcaa
     1621 ggtcaaatcg atatctatcc tgagttcaca ggtactgtcc tagaaagctt ggtgaaggta
     1681 cctgaagcaa tgaaaagtgc tgatttgaca aaagaaaaaa catatgacga agcagaagaa
     1741 ttattgaata agcaattcga tatgacatta ttaaaaccaa tggcttacca aaatacgtat
     1801 gccttggcag tcaagagatc ttttgcgaac gaacatcaac ttaaaaagat ttccgatttg
     1861 gcagctatct ctgatcagat caaagcggga ttcacactgg aatttattga tcgttcagat
     1921 ggatataaag gaatccaaga agcatatggc ttgaattttt caagtgtaca aagcatggag
     1981 ccaagtttgc gttaccaagc aatcaataat ggagatgtca atattgtgga tgcctattcc
     2041 acagatagcc agctcaagga gtacgatcta gtgactctag aagatgatcg agggttattc
     2101 ccagcttatc aaggcgcacc gctgatgaaa aaagattttg ctaaagaaca tcctgaaatc
     2161 gttaaagcac tgaataaatt aagcggaaaa ataacagagg atcaaatgat tgatatgaat
     2221 taccaagtca atgtcgaaaa gaaaaagccg acagaagtag cacgaatctt cctgaaaaat
     2281 gaaggattag taaaggagga agatcagtga ctaaattaat cgagttcaaa catgtgcaaa
     2341 aaaagtatga tggaaagtat gtcatcgatg acctaaatct ggcaatcaaa aaaggagaaa
     2401 tctttgtttt ggttggtcct tccggtagtg gaaaaacaac tactttgaaa atgatcaatg
     2461 gattatctaa accatcagca ggagacatct acttcaaagg aaaatcgtta aatgaatata
     2521 atctgcagaa aatgcggtgg aatatgggat atgtgctgca gcaaatcgct ttgttcccga
     2581 ccatgacagt caagcaaaat attgaagtca tccctgaaat gttaggttgg gaaaagcaga
     2641 aaagagcaga ccgtgtagat gaacttcttc aaaaagttgg cttatctcct gatatttatc
     2701 gagatcgtat gcctagagag ttgtctggag gagaacagca acggatcggg atcatccgag
     2761 ctattgctgc aagcccagat gtgattttga tggacgagcc atttagcgca ttagatccga
     2821 tttcaagaaa ctcattacaa gaattggttt tgtccttaca cgaagaattg ggcacgacca
     2881 tcgttttcgt tacgcacaat atggaagaag caatcaagct aggtgatcgt attgcattta
     2941 tgaaggatgg agaaattatc caatgtgaca caccagagca gttattgatg aatcctaaaa
     3001 acgactacgt tcggcatttc tttgatgaac cgaaacagac gaaagagtgg cgagtagaag
     3061 atttagtcgt caatggatat ttcttaaatg aaataccaga aaatgctcgt caacaagttt
     3121 gctttgatac gcctatgaaa gaagtatatt cgcttctttc ggtgtatcca agcattgtga
     3181 tagtagaaaa gcaaagaagt attgggtcgc tgacaagtaa agaaatcttt gcttttttaa
     3241 gtcgagagga ggaaggaaat gaaaacatat gatgcaatca tcatcggttc aggtgtgagc
     3301 ggcttaagtg ctgcttatgg attgaaagaa gcaggtaaaa cagtactagt ggtcgaagaa
     3361 gatctatggg gcgggacttg tcctaaccgt ggatgtgatc ctaaaaaagt cttgttaagt
     3421 gcagtagagg cacgtaatcg ggtaaaacaa ttgagtggaa aaggttttaa cgagatccca
     3481 actgccaact gggaagaact tcaaaaattc aaacgaacat ttacggatcc agtcccagaa
     3541 agccgaaaaa aacaattagc agaagctgaa atcgatcatc tttcaggaac cgctcgtttt
     3601 ttggatgatt catctattga agtcaacgaa gaagtcttcc atgcggatta cttagttttg
     3661 gcaaccgggc aaagacctac gatcttacca gtggagggaa aggaatactt gaaaactagt
     3721 gcagactttt tatcattgcc tgtactgcct aaagaaatca tttttatcgg tggtggttat
     3781 attgcttttg agctagctac gattgccaat gctgcaggga gcaaagtaac cattgtccat
     3841 cataatcaac ggccattaaa agaatttgag gcgtctttgg tagaggaagc cgtccatcaa
     3901 atggaagcat ctggcattca gtttgctttt ggtgttgaaa cccaaaagat catttcagaa
     3961 ggtactcgct atcgtttagt cggaaaagag acggaattag tagcagatat gattttttgt
     4021 gcgactggga gacagccgaa tacagaatct ttagcacttg agcaagcgaa tatcgtcttt
     4081 gataaacacg gaattgcagt caatgattat ctgcaaacga gcaatccaaa aatttttgcg
     4141 tgtggagata ttgtttcaag gaaaacccca aaattgacac cagtcgccac ttttgaagga
     4201 aattatgtag ctaagcggat aacggatgca acaagcgaac ctatcaaata tccgattata
     4261 cctacgatcg tctacgccag tcctaaactt gctgaagtag gagttacaaa aagtcacgcc
     4321 tcttcgtcag atcaagtggt agagatggat cttaccagtt ggttcaccta tcatcgtgtc
     4381 aatgaaccag ttgctaaggc tgaattgacc tttgaccagc aaaattattt gattggtgct
     4441 gcagtgatta gcgagcaagc agacgaattg atcgatgatt tgacactcgt catcaatcaa
     4501 aaattgacta aaaaagagtt ggatagctat atcatggggt atccgacatt ggcaagtgat
     4561 ctctcctatt tattgaaata atcagcatga taactaaagc gcttgttgct ttttccttct
     4621 tgtgctacac tttaaaaaat gaatacctgt ttctggggat gtcgtagacg acatgagagt
     4681 aagttgtgaa cttagaccca ttgaacctgt cagttaatac tggtgaaggg agaaacatag
     4741 cagacgcttg aagcgggtta ctatgtcgtg cccatttcaa gcgttttttg ttattcatat
     4801 tagaagaaag gaggtaacaa aatgaagaaa aatctttatc cagcagtcag tatggcattc
     4861 attttaatta tttggcagat tgttgtcata gtttggaaaa tacctgcctt tattttaccc
     4921 ggacctgctg ctgtattttc tgcattagcg gcagatgcac aagtattact ttcccattct
     4981 gtcacgacat taaatgaagc agtgcttggt ttggtaatag ctagcgttct ttcttttatt
     5041 actgccttgg caatggatta ttggaagttt ttagaaatgt ctttttatcc gttactagtg
     5101 atttcacaaa tgttgccaat catggtgtta gggccattac ttaccctatg gtttggtttc
     5161 ggtatgcttc caaaagtgat ccttgttgtc ttgataagct attttccgat cgtagtttct
     5221 ttttcggata cattgaggaa agtatcaaaa gagcaaatca tctttttgaa aacgatgggg
     5281 gcagatacag ggaagattta tcgaatatat aaaataccag cgggtttatc cggcttcttt
     5341 tccggtttaa aagtggctgc tacttattgt gtcagcggag cagtcgttgg agaatggcta
     5401 agcgctcaag caggcttagg gtattacatg atacgcgtaa aaaacagcta ccagatcgat
     5461 aaagtttttg ctgcgatcat ttgtgtcatt ctattgagtt tattgctgaa tggcgcttgt
     5521 tcattattga aaaaagggta ttatcgtatg ctttatattt tttaagagaa ggggatcaaa
     5581 tgaaaaaatt atggctttta ttaccgctac tgttgctttt atccgcttgt ggcactgcaa
     5641 aagaaacaag tagcaaacaa gaaataaagg atttgaaaaa agtgacactt gttttggatt
     5701 atgtaccgaa tactaatcat actggcatct acctagcaaa agaaaaaggc tattataaag
     5761 aggctggttt aaatgtccaa atcatcgaac caggagataa ttcgacaagt attggtctag
     5821 taggtgcgga caaagcacaa tttggtgtaa gttatcaaga agatgtcact tatgctcacg
     5881 cagatggaca gaatatacca gtcaaagcaa ttgctacagt gatcaaacat aatacttctg
     5941 gattcgccac cctatcagac agtaacatcc attcaccaaa agattttgaa ggaaaaacat
     6001 atgcaggttg gcaaagtcca agtgaagaag ctgtcttaaa agctgtgatg gaaaaagacg
     6061 gcggggactt ttcaaaactg acaatggtag gaagcaatgg agaagggcca gaaagcttag
     6121 gaaaatcctc agatatccaa tggtactttg aaggttggga tatgatcaaa gcaaaagaag
     6181 cgggtattga actaaactat atcccattga aagaattaga tgaacggtta gattactata
     6241 ctccggtaat tattacgaat gatcaactga tcaaaagtga tccagaattg gtacagtctt
     6301 ttatggatgc cacgaaaaaa ggctatcagg aagctattaa ggatccaaat gacagtgcta
     6361 aactgttgca aaaatatgca aaagaaaatg atcggacgtt tttagaagaa tcacaagcat
     6421 tcttgtcaaa aaattatacg gatgatcctg agaattgggg attgatggaa gaaaaagttt
     6481 ggcgcaatta tacagctttt atgcaggaaa acggattgat caagcaagat gtaccttcgc
     6541 aagaactatt cactaatcag tttataaaat aggagtgata gatatgtcaa tccaaatcag
     6601 ccacttgaat gtagtcattg aaaacctgaa aatattagaa gacatccatt tcactatccc
     6661 taaagatacc tttacatcga ttgtcgcgcc aagcggagcc ggtaagtcaa cattgctgaa
     6721 aactttaaca ggagtccagc ctatgacttc tggaacgatc aaagtagacg atcaaaaagt
     6781 aactggtctc catacagcat ttagttatat gccgcaagaa gatatgttac ttccttggct
     6841 gagcgtctat caaaacgtta cgttatatca aaaaatcaat catctaacaa tcaatgaaga
     6901 acaagtaagg gattatctgg atatctttgg gttaaacggc tatgaacatt ttttgccgga
     6961 acaattatct ggcgggatga agcagcgaac ggccttgtta cgaacaatca tgaatccttc
     7021 ttcttacttg cttcttgatg aaccatttgg agcgttagat gctatgacca gagggcagat
     7081 gcaagactgg atgttgaagc tgcctaaaaa agcaaagcgg actactttac ttgtgacgca
     7141 tgatatcgaa gaagcaattt atttatcaga caggattctt gtgctttccg cccgtccggc
     7201 acatgtgatt gctgagattc aagtgcctga aaaacgacgg tcacgtgaat ggctactgca
     7261 gcaatcagag ttgaagcaaa cgatttatca gttattggca ggtgaattag atgttaagtg
     7321 atgctcattg tcatttagat gggagtcaga gtttagcact ccttcagcag gaacacacga
     7381 ttttgacgat catcaattgt gattcgccag aagaatggaa ggaaaacaga cagcttgcag
     7441 caagtaagac tcaagcctta agttacggta tccatccttg gaaagcagat agctatacgt
     7501 ttgaacaagt tgagcctttt ttgaaaaaag caaggatcat cggtgaaata gggttagata
     7561 atatttggac gaatgtaccg atgactacac aaaaaaaagt atttgagcga cagcttgctt
     7621 ttgcagccat aaatgagaaa cctgttgtac tgcacacaaa aggatgcgaa aaagagatct
     7681 taaattatat ccaagcttac cctaatcact atttgatcca ttggtattct tctttggact
     7741 atcaacaaga ctatattgat ttaggctgtt atttcagcat tggtcttgat ttgaaaaaga
     7801 atccagcagt ctggcaacta gctaaagcag tcccaatcga ccacttactt attgaaacgg
     7861 atggactaca tgcagcagag tgggctttag gggggatttt ttcagaaaaa gattatgtct
     7921 cactgcttga agaacagtta aagacaatcg ctgagatcaa agagctatca gttgaagaag
     7981 ttaaaaaaaa tacttatcaa aacttggaac gtttcattag attaggtaaa taaaaaagag
     8041 atgtgacaca actagtgtcg cacctctttt tggctttctt tgtataaaag taaactgttt
     8101 gattaataaa tgaatcttag taccaaccgt ttgcttccca gaatgctttc gccgcttccc
     8161 aagaaccata acgtgaagta acatattgtt ctgctactct ttcttggttt gcagcagagt
     8221 agtcaccatt taagtaagaa gaatctaatt ggtaacgtcc gatgtaacgt ccgttcgttg
     8281 ctgtgtaaga accgcttgat tctttttgtg cgatccattc ttttgcagag cttgtgcttg
     8341 ctggagtagc agcagtttct gttacagcag gagcaggttg tgtttcagca acaggcgctg
     8401 cttgtttttc cgtttgaacc gtttcttgta caacaggttg ttcagcttgg actggtgctt
     8461 cttgaacggc tgcttgaggt tcagcagctg cttgtgcaga gccttctgtt gggattgtta
     8521 attgttgtcc tgagta
//
LOCUS       DAZKLA010000117         8394 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000117 DAZKLA010000000
VERSION     DAZKLA010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.119"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..477
                     /locus_tag="ACG85N_002334"
     CDS             55..477
                     /locus_tag="ACG85N_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0225055.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(643..1836)
                     /locus_tag="ACG85N_002335"
     CDS             complement(643..1836)
                     /locus_tag="ACG85N_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0225056.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            2060..2437
                     /locus_tag="ACG85N_002336"
     CDS             2060..2437
                     /locus_tag="ACG85N_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0225057.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2425..2763
                     /locus_tag="ACG85N_002337"
     CDS             2425..2763
                     /locus_tag="ACG85N_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0225058.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            2897..3340
                     /locus_tag="ACG85N_002338"
     CDS             2897..3340
                     /locus_tag="ACG85N_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFQ0225059.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            3602..4417
                     /locus_tag="ACG85N_002339"
     CDS             3602..4417
                     /locus_tag="ACG85N_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0225060.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            4427..5089
                     /locus_tag="ACG85N_002340"
     CDS             4427..5089
                     /locus_tag="ACG85N_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0225061.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            5294..6958
                     /gene="alsS"
                     /locus_tag="ACG85N_002341"
     CDS             5294..6958
                     /gene="alsS"
                     /locus_tag="ACG85N_002341"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFQ0225062.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            6971..7681
                     /gene="budA"
                     /locus_tag="ACG85N_002342"
     CDS             6971..7681
                     /gene="budA"
                     /locus_tag="ACG85N_002342"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFQ0225063.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            7811..8308
                     /locus_tag="ACG85N_002343"
     CDS             7811..8308
                     /locus_tag="ACG85N_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFQ0225064.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         2815 a         1416 c         1699 g         2464 t
ORIGIN      
        1 atccgttttt tttcttgtat gtagtatagt aaagtagata caggaggaga aaatatgcag
       61 acttttttaa caaaagtttc tcaaaagaaa attaaaatga ttcatttact gcttgaaaca
      121 gaacgttggt gttcaataga agaactacaa aacgaattga aggtatcaag taagtctatc
      181 ttgaattatc taacagattt agaagaattg ttccaaaaat atcctgataa ggtcattttg
      241 aagaatgaga ataatcggcg cttttctatg gaaaagcagg agaacttccc aatctacatt
      301 atttatcttc atttctacag aaaatcatat aattatcatt tgattgaatt catgtatcaa
      361 catcctgaga aaaatttgga agattatgca gacgctcaat atacgagtgt atccacagtt
      421 tttcgttatg ctaagttatt agtaaagtac tttgaaatga cggacgtcaa agaatagtcg
      481 ccgccagata gaaggaaatt agcagttgaa aaagctgccc tgcatcaagt aatacgacca
      541 gccaaacgga aacagtgtct catgtttttg atttggctgg tcttattacc gcagatgcaa
      601 gcttttgcca cacctctttc ttgtcattta cagactcaac tatcaatgct gcaagctttc
      661 ccttaatgct gtaacaccag taagagttgt caagaccagc agactgacta ggtaaatacc
      721 gctcaaaaac aagattacgg cagttaaact ataatgatcc atcaagaaac cgatgacgat
      781 agaagaaaaa ccaccaacag ctcgtccaag gttcataatg aaattattcg ccgtcgcgcg
      841 gatttctgtt ggatacaaac tactaatcac tgcgccatat ccaccataca tgccattgat
      901 aaagtagccg actatcacag cggctgctac caaactccac tggctatggg ctaacacaag
      961 tagatagaca gataaggatg aacaaattaa aaacaaagaa aaggctagtt taggccccaa
     1021 tttatccatg attatgccaa aagtcatcat ccccaaagac atgcctaaaa tagtactgac
     1081 catccacaat gaagacccag aaaccgaaag ccctaatcga ttttgcataa ttgaaggcag
     1141 ccaattcatt aacccaaaat agccggcaat ttggacagtt accatgatac taaggccaca
     1201 tgtttgccaa gcaagatgtt ttgattcaaa cagtctgcta agtttggttt tcttttcggc
     1261 agattgtaca gatttgaatt cctttggttc agtcaagccg cgacgaatga acaagaccat
     1321 caacaccggc agtacaccta cgacatacaa catcttccaa ccaaacgttg gaatgatcac
     1381 agcagctaaa atcgcagcca aagccgcacc gacctgcgca ccaataccag caaccgaaga
     1441 agctcgtcca atttgttttt ttgaaaaact ttcggaaatc aaggacatac acgccccgta
     1501 ttctcctccc gctcctacac ctgcgataaa gcgaaagaga taaaccaagt agatgtttga
     1561 agcaaaatac atcaataaag aagcaatcga aaacagaagg accgtttgag aaaatacttt
     1621 cacacgtcca tatttatcag ctatgatgcc aaaaaaaata cctccaatga gcatccccaa
     1681 attagtgatc gttgaaatga aacccgcttg cgtaccacta atatgaaaag aagtaatcat
     1741 tgaagataat gaaaaagcta aaaacataat atccatattc tctaaagcaa ttcccgccga
     1801 tgtcgataat aatacctttc gctgatacgt tgtcattttt ataccccttt tcctaaaatt
     1861 tccctaaatt gttaaaagta tatccaaaaa agaataaaaa aacaagactt ttttatttga
     1921 aataatacac aaacaaccga tatagataat ccagcttagt tttaatagaa atgagtattg
     1981 acttggagta cacttaaagt tgtagaataa ataaaataca taagtaaagt ggcaagaaat
     2041 taagagaaag agtgatcaga tgaatatcaa acaagtcagt gaagaaaagg gcatttcggc
     2101 cgatacattg cgctattatg aacgaatcgg gttgattcca ccagttaatc ggacaaaagg
     2161 cggaatccgt gattatacag aagaagacct aaaatgggtc gactttacga tctgtatgcg
     2221 aaatgccgga ttatcgatcg agtctttgat tgaatatatt cgcctatcta gcgaaggaga
     2281 agcaactgta tttgagcggc gtaagctttt gattgaagaa agtgaacttt tgacgaaaga
     2341 aattgctgaa atgcaagagt gtttggaacg tttacaagga aaaatcaaga aatatgatcg
     2401 tgtgttatct agaaataaaa tggaatgtat caagtaaaaa aatcaaggag catcgctatg
     2461 gagttacgct tactaaaata tttttggaca attgcagaag aaggaacaat ctctcaagcc
     2521 gcagaagttc tacatcttac acaaccaacc ttaagccgac aactgaagga gttagaagag
     2581 gaactaggaa cagaactgtt tgtacgcgaa aaccgcaaaa tgatcttgac agaagcaggt
     2641 tattttctaa aaaagccgag cagaagaaat tttagattta acgaatcaaa ccactcaaga
     2701 attcgagaac agaaaaaaac aattattcag cggccgattt acgatcggct gtgtggaagc
     2761 tgataattcg aatacattgg cactattact agaaaagttg atccgtgatt atcccagcgt
     2821 tcaatttcat atccacagcg gaacaagtga tgatatcacg gagtgactag aaaaaagcct
     2881 gttagattta gcgattttgc tagatccagt agatacagag aaatacgaaa cgattttgtt
     2941 accaagaaag gaaaaatggg gattgctcat tttagaaaat tctgttttag caaaaaaaga
     3001 cgtgataaca aaagaagact ttgcgaatgt acctcttttg atctcaaagc gtccagaaat
     3061 acagcgaatg tttgctgact ggttaggcat aaaaaaggag cagttgctga tcacaggaac
     3121 gtacaatttg attttcaatg tatttgcgct aattgagaat caggtaggtg ctgctttagt
     3181 catagaaggt gctgtaagtc atctttcaaa gcgagtgaca ttcgttccgc ttgacccaat
     3241 gattgaaacg aactgcatgc tagtctggaa aaagaatcgg attttatcac ctgtagtcaa
     3301 tgaattgatt caccgtttca aagaagcttt tgaacagtaa taagtatctc taatcgaaga
     3361 aatttcaata caaaagcgag acgtatgaca gtcttgcttt tgtatttttt catttttaaa
     3421 tcaaaaaaaa taaagagatt tacataactt gttaaataag cgcttacaaa cataagaaga
     3481 acggaaatga atcagctata ataaccgttg ttctggattg taatcattac aaagaaatga
     3541 agattataac tttggaggtg ttcgaaatga aagaaaaaaa gcaaaaattc gttcaaaaaa
     3601 catgcccttt attcaatggt tgggtatgat tctgctgatt gtattagctt tattgacgat
     3661 cattcctttt ttatggatgg tttctgcttc attcaaagca aataatgaag tgttcacgat
     3721 tccgattcaa tggatcccta aaacttggca tttagagaat tatctggtta tttggtctcg
     3781 tattccttta gtgacatttt ttaagaatac agctttttta agcgttgtga tcacgttgat
     3841 tcagttgttt acttctagtt ttgctgctta cgggttttct aaactaaatt ttaaagggcg
     3901 cgatatttta tttttagcat atatcggcac gattgctgtc ccttggcagt cttatatgat
     3961 cccgcagttc attatgatga gaaaagtggg tttaacatat acgttgtggt cgttggtgct
     4021 attacaagcg ttcaatgctt ttggtgtgtt cttgttgaag caatactaca gtagtatccc
     4081 agattcttta tgtgagagtg cgcggatcga tggcttatca gagtggggaa tctatcgcaa
     4141 gatcatttta cctcttacaa aaccggcgat tgccagtttg acgatcatca cattcgttaa
     4201 tacatggaac gattatatgg ggccattcat ttatttgtct tctacggaaa ataaaacaat
     4261 ccagttaggg ttgaaaatgt ttattgggct atttgatgca gagtacgcat taatcatggc
     4321 tgcctcagtt gtttctgttc ttcctgttgc ttttatattt ttagcgatgc aaaaatattt
     4381 tgtagaaggg atcgcttcat caggaatgaa ggaataggag gtgattatga aagagcagcg
     4441 ttttgacaaa aatgtttata tggaattctt ttattggttg tatcaatcat tggcaagtag
     4501 cctttgtttt tggtgtgtca atatcttgta tatagcagct gcgttccttt tgtcgataga
     4561 tggaaggaat gtcttttcct ttattttttc tttacttttt attggtccag gttgcatcgt
     4621 gatgataggt atgacagaag aattagcaaa agaaaaaagt ttttcaccag tacgtttgtt
     4681 cataaagacg tttcgccaat tttggctaaa gggatttttt tattggctat ttgcttggat
     4741 agttagtgtg ataatgattt ttgattgttt cttttttata cgtttttctt atggaaaatg
     4801 gttgattccg ttgtttgttt tacttgcttg tttatcagtt tcctttagca tcaactactg
     4861 gtattttcag gtgagaaatc cagcgtcgaa accaaatcaa gttctacgga tagcgttcta
     4921 ctatacgttg aaaaaatggt atgtgagttt actagatttt ttactattga caagtttgtt
     4981 tctcttcttt ttcgtaaagc cccaatggtg tattctacta ggtccttcta tcgtatttgg
     5041 tttgatttac ttcaacaatc gtaaattgat gagaactatg gatctgtaga ctttataaat
     5101 tgaaactttc aattaagaat gacagttttt atgtaaaaac tgtcattctt tttcattttt
     5161 tgaaacatat ggctaaacaa atgaaataaa acgtgctata cttttgaatg aaacaggttt
     5221 agaaactgta tcaattattt gtgattattt ctaactaata attaaaaata aagcataaaa
     5281 aggacgatgt tcaatggacg aaaaaatgaa acaaggttcg aaatctgtag tagaaagttt
     5341 gattaatcat catgttgatt atgtattcgg tataccaggt gcaaaaatcg atggtgtatt
     5401 taatgaatta gaggatcaag gtcctgaatt aatcgtgact cgtcacgaac aaaatgctgc
     5461 atttatggca caagcaatcg gacgtattac aggagagcca ggagtagtta tcgcaacaag
     5521 tggtcctggt gctagtaact tagctacagg tttggtaaca gcgacagcag aaggtgaccc
     5581 agttttagca attgccggac aagtaaaacg aagcgatcta ttgaagttga cccatcaaag
     5641 catggataat gcagcactgt tccagccaat cacaaaatac agtgcagaaa tccaagatcc
     5701 agaaacaatt tcagaagtga ttgccaatgc ttatcgtatg gctaagagtt caaaaaaagg
     5761 cgcgagcttt atcagtatcc cgcaagatgt agtggatgca cctgtccaag ggaatgtcat
     5821 caagccattg agcgatccta aattaggttc tgcttctgca gatgatatcc gttacctagc
     5881 tgaacgcatt agagaagcaa aactaccggt actattagta ggtatgcgag gatcaagtga
     5941 aaaagaaacg ttagccatca ggcaattagt tggaaagaca gcattgcctg ttgtagagac
     6001 attccaagca gctggagtga tctcacgtga attagaagcg catttctttg gtcgtgtggg
     6061 tttatttaga aaccaaccag gagatatgct tttgaaacga agtgatctag ttattgctat
     6121 tggctatgat ccaatcgaat atgaagcaag aaactggaat gcggaaaaag atgcgcggat
     6181 cattgtgatc gatgaagcgc cagctgaaat cgatcctttt atgcagcctg aaagagaatt
     6241 gatcggtgat atctcagcca ctcttgattt attgacaggt tcattagagc ctcaacaagt
     6301 gtcagaagat gcaaaagaat atttagcatc tcttcaagca gaattaacgg agcgggacat
     6361 tgttcaatca aaaggagaag caggtatctt gcatccttta gaagttatta acacgcttca
     6421 gtcaaaagta acagatgata tgactgttac cgtagatgta ggtagtcatt atatttggat
     6481 ggcacgccac tttagaagtt atgaacctcg tcatttgtta ttcagtaatg gtatgcaaac
     6541 gcttggtgtt gcattgccat gggcgatttc tgctgcattg gtgcgtccaa atacacaaat
     6601 cgtatcagtt tccggcgatg ggggattttt attctctgcc caggatttag aaacggcggt
     6661 acgtaaaaaa ttgaatatcg ttcatttgat ttggaatgat ggacactaca atatggtaga
     6721 gttccaagaa aaaatgaaat atcagcgggc gtcaggtgta gactttggtc cagttgattt
     6781 tgtgaaatat gcagaagctt ttggcgctaa aggaatccga gctacaagtg tagaggaatt
     6841 agaaaaagct ttagaagaag gatttgcgac agaaggaccc gtaattattg atattccgat
     6901 tgattaccgt gataatgaga aattgggaga aacaatctta ccagatcaat tctattaagg
     6961 aagtgtcgac atgactgaaa aaatcttata ccagcacggg acattaggag ctttgatggc
     7021 tggcttgatg gatggaacag aaacgattgc tcatatatta gaaaaaggaa ctctgggatt
     7081 gggaacattg catgggcttg atggtgaagt catcttttta gatggcgtag cttatcaagg
     7141 aagatcagat ggctcagttg cccaattaga tggaagtgaa cttaccccct atgcagcaat
     7201 tacagatttt acgccagata ctagtttttc tgtctcaaaa actgcagatg gagaacaatt
     7261 gaaaaaagac atattagtaa gagaagccgg ggaaaaccta tttttggcag tgaagataac
     7321 tggattattc aaaaatatgc acatccgtat catgccgaag caagaaaaac cttacagaag
     7381 actagcaaaa atttcagagt ctcaaccaga attccaacaa agcaatgtcc aaggaacatt
     7441 agtcgggttc tttacgcctg aattattcca aggagttgct gcagcgggat ttcacttgca
     7501 ttttatcgat gatacacata catttggcgg acatgtaatg gattttgaag tggctgaagg
     7561 aaaagttgag attagccgca tctcgtccct tgtacagcat tttccagttg atgatccgac
     7621 ttttgttcac gaaacgattg attatgctga tttagcagca gaaatccaac aagcagaata
     7681 gaaaaagaca gcgagatgcg ttcaaggtga ttaacctgac gtatctcgtt tttttgagga
     7741 ggaaacctag cgtttacaaa aaaactgagc tataatcttc tccatagatc aaaaaggagc
     7801 ggtttttatc atgtggacag aaaaacagaa aaaagatgca tacgaaattt tattattaca
     7861 gcaacaagga ttattagaag cagaagataa ctggttagca aatttagcaa attcatctgc
     7921 attgttgaat gagactttgc ctgagactgt ctttgcagga tattatttat tcgatggtaa
     7981 tgaattgatt ttaggaccgt tccaagggcg agtttcttgc acacgtatca agatgggaaa
     8041 aggagtttgt ggagaatcag ctgaaaaaca agaaacattg atcgttgatg atgtcaaaac
     8101 ccatgcaaat tacatttcct gcgattcagc cgcacaatca gaaatcgtag tgccaatggt
     8161 aaaagaaggt aatttaatcg gtgttttaga tatcgattgc ggagtaacga aagggtatga
     8221 tgaaatcgat caagcatttc tagaaaaata tgtagatctt ttattagcaa attttaatat
     8281 aaatgctttt tcatttatta ataactaaaa tcgtctattg aaacaaaaaa gaagagaaat
     8341 caagaataaa aagagccgga aagacagtgg gcatttctgt ttttcggttt tttt
//
LOCUS       DAZKLA010000118         8344 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000118 DAZKLA010000000
VERSION     DAZKLA010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.120"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..429
                     /locus_tag="ACG85N_002344"
     CDS             <1..429
                     /locus_tag="ACG85N_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0225065.1"
                     /translation="IKNEYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIR
                     VLGIILDNAIEASIESQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTK
                     KGHQGLGLSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
     gene            444..1196
                     /locus_tag="ACG85N_002345"
     CDS             444..1196
                     /locus_tag="ACG85N_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0225066.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(1899..2114)
                     /locus_tag="ACG85N_002346"
     CDS             complement(1899..2114)
                     /locus_tag="ACG85N_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFQ0225067.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(2133..2369)
                     /locus_tag="ACG85N_002347"
     CDS             complement(2133..2369)
                     /locus_tag="ACG85N_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFQ0225068.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            2627..4780
                     /locus_tag="ACG85N_002348"
     CDS             2627..4780
                     /locus_tag="ACG85N_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFQ0225069.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            4749..6164
                     /locus_tag="ACG85N_002349"
     CDS             4749..6164
                     /locus_tag="ACG85N_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFQ0225070.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            6197..7231
                     /gene="sppA"
                     /locus_tag="ACG85N_002350"
     CDS             6197..7231
                     /gene="sppA"
                     /locus_tag="ACG85N_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0225071.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            7233..7754
                     /locus_tag="ACG85N_002351"
     CDS             7233..7754
                     /locus_tag="ACG85N_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0225072.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            7751..8137
                     /locus_tag="ACG85N_002352"
     CDS             7751..8137
                     /locus_tag="ACG85N_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0225073.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         2974 a         1198 c         1501 g         2671 t
ORIGIN      
        1 ataaaaaatg aatatttgaa aagtttgttg atagcgaagc tataccaagc aaataacaat
       61 aagatttgtt gtaaattcga atgtccaagt actattattg aagttcctgt tccaatattt
      121 gactgtatta gggtgcttgg aattattctc gataatgcaa ttgaagcatc tatagagagt
      181 caagatagag aactgtctct agctatttac cgagatgata aacagataga aatttcgata
      241 tcgaatagtt gtagagagat actactctct atagatactc taatgaaaaa aggaataaca
      301 acaaaaaagg gacatcaagg gttaggttta tctagtatag aggaaatcaa taaaaagaat
      361 cccaatatgt ttgtgaacta tcaaaacgat ccgtatcaat ttactactca agttattttg
      421 ttgttttgag ggaaggagga tttatgagtt atccaataat attatgtgaa gatcaaatta
      481 tacaattaaa tcagttagaa cgaattattg ataatttcat tctttttcac gataaagtat
      541 ttaagatagt attgaaaaca caaagtccgt tagaagttaa aaaatacctc aaacaattcc
      601 gaccaaagca aggaatatat ttcttagata ttgatttaaa tcatgaagtt aacggtatag
      661 aattagcaga agtaatcaga aaatatgatg ttcaagcaaa aatcattttt acaactactc
      721 atgatgagat gttacccgta acaataaaaa gaagagttga aacgttagga tttgtaacaa
      781 aagatcaaac actagatgag tatcgaaacg agattgttga gttattgtta ttagcgcaag
      841 aaaggataga tgcaacaaaa caggttacga atcaagcatt tgttttttcc ataggttctc
      901 aaacatttac ttttgatata aatgacattt attttgtaga agcttcaaaa ttaccacata
      961 gattatcatt gtgtactaaa gatgggcagt acgaatttta tggaagaatt agtgagcttg
     1021 aaaagaaata tcctatgtta acaagaatta gtcgagcgtg tttagtaaat atctttaacg
     1081 ttaaagaaat agattttaaa aaacgttcac tatattttga ttcagagttg gccagaaact
     1141 ttactttagg gaaagcacaa aaaattaagg agaagttgaa agaaagcact gtttagtggt
     1201 tttttcttta gggagattgc atttttctaa taaaatagat tccttcacta gtgcaaattt
     1261 ttctgaatcc tattaaagtt gtaccaattt ttatagctgt tattagttac gtgcataaca
     1321 gcaatattag ttactggagg aatatttcta tggtctggaa ccaaaaaagg gccaaatgaa
     1381 tagttctttt cttgttttta ttaagtacca taaaggggaa ttaaaattaa atgtatggtg
     1441 gaagatgcga aagatttggc aaattctact aatttttaga aagtgttttt cattttttag
     1501 ttaaaaaatt tgtcctgtca agtgcaatcc tatgattgta cttgatagga tttgagataa
     1561 ttactttcct gtttttataa atttaacttg caggaacagg ctttaatgca catccgacaa
     1621 gtacattacc aatacctcct gtaacggccg aaataggtcc tcctgcagca ccaactacag
     1681 cgctgcaagt ccttttgtaa aaatatctct accattacat ttccaaagaa tttttatctg
     1741 actttaataa aagataaaaa tttaatataa ttataaaaac tagcacaaga agctttagta
     1801 aaaagttctg tccattccaa acttttgaaa agataaagaa catacctatg ttcgtaatcg
     1861 acacaaacca atcttgaggt ttgatatttt ttaattgttt acgcaccggt ccacatgctg
     1921 aatccagcac ctaataaagc tccacctaca ccacaagcag ctattgcata aggtcctccg
     1981 gctgaacaaa gttttactcc tgctgttgcc ccagaaaaag tagtgcctaa aactcccacg
     2041 gcactcccac cttgaatatt tttcaactct tctgaagata atataaatgt tgtttttgtg
     2101 tttttaatat gcatactact tattctcctt ttttattttt ttcgagcatc ccagccagct
     2161 gcagcacctc cagcgacagc tccatataac cctccaacaa caccgcaagc tggtccgcaa
     2221 attgctgttc ctaaacctgc accacctact gcccctgtgg taagtccacc tacaacttgc
     2281 cccaattttc cccaagcatt attgcctcca ccaacagttt gtgcatttcc acaatactta
     2341 gttcctttac attttgcatg tttaaacacg cttctttaaa taatattttt tacggtgcta
     2401 ctgtaacata cgtatttgat taacagtaaa aatcttcctg aaacttatca agtttattct
     2461 tgaatagtaa aatttccaaa ataaatttga ttcaggaatg aaaaagttag tgttcaagaa
     2521 ctgttttcaa agtttttgtg gaattatgag acaatatttt cactgtatta aagctatatt
     2581 gagaattttt ctataaaaat tttttagaag gagttgtgtt agataagtga taaaatcatt
     2641 tccttttgtg cagcaacaag atgaaaaaga ttgtggtgtt gcttgccttt cgatgatttt
     2701 aaaatatttt aaaactgata tcccgattca taaattacgg gacctatctg ggactgattt
     2761 agagggtact tcagcatttg gattgaaaaa gacatttgaa aaattgtctt ttgattgtat
     2821 ggctattcaa gcagatagtg agatctggaa agaaagagat cttatcttac cattaattgc
     2881 acatatattg gttgataaca gctatatgca ctacgttgta atttataaaa cgaagggaaa
     2941 cgtactatgg attgcagatc ccgctaatgg aaaaaataga aaaacgattg aagcgtttca
     3001 aaaagaatgg acaggtattc ttttattacc tatgcctaga gaaacttata ttcctattaa
     3061 agaaaaagta gctggattaa gttctttttt gcctataatt tggaaacaaa aaggattagt
     3121 attccacatc gttttagcat cgattttcat tacgtttttt ggcataggaa gctcatatta
     3181 ttttcaaggt attcttgatt ttttcatccc taatcaagca cgttcaactt taaatataat
     3241 ttccattggc ttgatcgctg tctatttatt tcgggtattg tttgaatata gtcgtagtta
     3301 tcttcttatt attttaggac agcgaatgag tatggcaatt atgcttcagt atttcaaaca
     3361 tgtgttaact ttaccaatga atttttttgc tacgagaaaa tcaggggaaa ttatttctcg
     3421 ttttcttgat gccagtaaga ttattgatgc tttagcaagt gctacgttat caatattttt
     3481 agatattggt atggtgttgc ttgttggggt tacgttagct attcagaata gtacgttgtt
     3541 tttaatcact ctatgctctt tgccttttta tgtagttgca atattcgctt tcgtgaaaag
     3601 ctatgaaaaa tcgaacaggg aagaaatggc agccggtgca gctgtaaatt ctagtatcat
     3661 agaaagtctt aaaggaatag aaacaattaa agcatatagc ggtgaagaga aagtatataa
     3721 ccgtatagac catgaatttg ttaaattaat gaaaaaatca tttcgtacgg ttacgcttga
     3781 taatattcaa caaggaatta aacatttgat tcaattaatt agcagtgcat taattttatg
     3841 gctaggttct tattttgtta tggataatag aattagtcta ggtcaactta tcacatacaa
     3901 tgctttacta gtatttttga cagatccttt acaaaacatt attaatttac aagtgaaaat
     3961 gcaaacggct caagttgcaa ataatcgttt aaatgaaatt ttcgcgattg attcagaaaa
     4021 atcgaaccag tcttttgaat atatgctttc tgaacaatta tttcaacaag atatcactgt
     4081 aaaaaacgtt tcattctcgt atagcatgaa ggccccaacg ttgaacgaaa tttcatgtac
     4141 aattccttcg cacagtaaaa cggctatagt tggagttagt ggttctggaa aatcaactct
     4201 tgcaaagtta tttgttcgtt tttatgaagc atcagaagga tcgatccatt atggaaacat
     4261 taatcactta gacattcctt ttcagatatt acgcgatcga gtgacatatt tgcctcaaga
     4321 gtcatttttc tttagtggaa caatcattga gaatcttata ttcggtttag atagcccacc
     4381 aagtttcgaa caaataatag aagtttgtga gatagtccaa ctaaatgaat ttattaatca
     4441 acagccatta cgttacgaga cattgttgga agaaggtgga aataatcttt caggagggca
     4501 acgacaacga ttagccttag cacgggcttt attaaaaaat ccggacatat taattttaga
     4561 tgaagcaact agtggattag ataccttgtt agaacatgca attatgaatt atctcctcaa
     4621 tttaaaagaa aaaacgatta tttttattgc gcatcattta ccaatagcaa aagcatgtga
     4681 tcaaatttta gttctccatg aagggcattt ggtagaaaaa ggaacgcacg atgaacttcg
     4741 ttttgccgat ggtatctatc aaaaattatg ggaaatctag gagagggaaa caatttatgc
     4801 agaataataa ttggttcgaa cattctagta tctatactaa acaacatcat agcttctacc
     4861 gttgggtgtt ataccctgta attctttttt tatgtattat tggattgttt ttaacctttg
     4921 ctaaaaaaga ggttgttatt cggacaacgg ctcaattgaa ccccgaaaaa atagagaagt
     4981 tacaagtgcc attagaagct aaaataatgg aaaacttcct aaaagaaaat caatttgttc
     5041 ataaggacga tgtgttggtt agattggatt gttctttaat agagaatgaa aaggctcaga
     5101 tagaacaaga aaaccaacgt attactcaac aaataaagat ggctcagcta tttattgaaa
     5161 gtataagtaa aggaaaaaat ttgttttcaa cggatgacag ttttggctac agtaatcaat
     5221 taaagagcat gttgtcagaa aaagaatcac tccgctacgc tttgaagcaa agtgaattaa
     5281 atgatcaaaa gcaattagaa gtatacgaaa agacaaaaag acaactagaa aaacaaattg
     5341 agagttcaga tagtaaatta caagaatggc aacaagtaca ggtagcttgg agtaataatc
     5401 aatcattaaa agatttttca aaagaaatga tggcaaacta tgagaattgg caagaacaac
     5461 taaataatgt ttctgatgat caaaaaaatc aagtgaaact gacaatttca gcaagcataa
     5521 atgaacaaat tgagcaacta aaaaaagaag tagaacagta tcagtcagaa aaagctaaat
     5581 tagttaaacc aactacttct gagaatgaca gaattagtca aacggaaaaa ggaaagcaag
     5641 agctagaaca agcaataaca actgtaaaac aaacagttac agagctaaat gaaaaacaag
     5701 agaagaacca gtcagttatc aaaagtttgg acgatcaact ttcaaaagga atactaaaag
     5761 ctcctgtatc tggaacggtt catttaaatg aagaaacaaa gggacaagca gaactagcca
     5821 aaggaacagt gcttgcagaa atttatccga tccacgaaaa atcaaaaatg aaatttacag
     5881 cacttttacc tgcgaatgaa agtacctata ttaaagaagg gatgaaggtt cattttaaat
     5941 tggatcaaaa aggtggggct ccgattacga tcgacggaac tttagatgaa atttcagaaa
     6001 attctactgc cactgagaaa ggtgtttttt atgcagtgaa aggaacgtta aaaacaactc
     6061 aatcgtttcc gtttcgttat ggattaactg gagagttgtc tttaattgta ggagaaaaaa
     6121 catattggca acaaattgtc gatgtgtttc taaataaaaa ataattattt ttactaagaa
     6181 tataggagga gtttatatga ataaaagaag atggattgct gtgggagtag cgtgcggatt
     6241 attgttacta tccattatcg tcgcgctaat tccaggaaaa gataaagagg aagcatcaaa
     6301 tacaacccta acaggaataa ataagatttt ttacggatct aacgaaatca cagaagaaac
     6361 gttagaagaa ggcgcatcaa ataaaaagat tgtcaaacta tccgtcaatg gagttattgc
     6421 agatacaggt gaaagcaatc tttttagtag ggaacaatat aaccatcaga attttttaac
     6481 acagctaaag aaaattcaag aagacaaagc ggttaaggga gtgttattag aagtaaattc
     6541 acctggggga ggaatatatg aaagtgctga gattgctaaa gaaatggcaa aaattaaaaa
     6601 gctagatatt cctatctata ctgcatttaa aaatacagcg gcaagtggag ggtactacat
     6661 ttctgctggt tcagataaaa tatttgctac ggaagaaaca acaactggat caattggtgt
     6721 cattatttct ggtttaaatt attctggctt attagaaaaa cttggcgtaa ctgatgcgac
     6781 gtataaaagt ggtgcattaa aagatatgat gtcacctcaa cacaaacctt cagaggaaga
     6841 gaataaagtt atacaggaat ttgtgatgag tgcttatgat cgttttgtca atgttgtagc
     6901 aaaaggaaga aatatggata ctaatgcagt gaaagaactt gcggatggaa gaatttatga
     6961 tggtaaccaa gcagtagaaa atgggttggt ggatcaaatt ggttactctg aagatgcatt
     7021 agattcccta aaaaaagaga agaaattaac tgatgcgacg gttatagaat ataaaaatga
     7081 cacaactggc tttgcttctt cttggttagg taataagatt gctgaatggc aagggctaaa
     7141 ggcaactaca tcagatcgtg taatttcagt ttttgaaaaa cttggatttt ctgatactcc
     7201 aaaaccaatg tattactatg gagggctata aaatgaataa gaagagtggc attgaaactt
     7261 tgcctaatta tgtatttgcg ggattttggt tacgattttt tgcttttctt attgatttaa
     7321 tttgtattag catcatcacc catgctacta taggtctctt ttacaaaatc atgggactgg
     7381 aatctgacaa atccattatt actttttatg gtatagcgtc attagctatt tatttaacct
     7441 attttgtact tttgactaag ctgaattcag gacaaacaat tgggaaaatg atttttggga
     7501 taagagtagt gagctttgag gaaaaagagc taagttggca aactatcttt attcgtgagt
     7561 gtatatgtag gtttatatta aaaaattttg tatttcaaat tgggtatttg gtggctgctt
     7621 tcacgcccaa gaaacaacat attggggatt atttttctaa cacgtcagtg gttgttattc
     7681 gttcattaga agctattaac tatggtcaaa aaatttctaa ttctaaaaca cttaatctgg
     7741 agagaattaa atgaataaga gaaaaacaga gtttattttg attttaatta gtggaataac
     7801 agggatttta tgtagtcttt tagcattgat gggtggtatg tttctatctt ctattgattc
     7861 aactacttta aatactacag aactaaccag taaggatata gatatgttag tcaatctaag
     7921 cgggaatgtt gttttatttg gaggaattac cctagttttt tcgataatga ttttcataat
     7981 aggtttttta gtaagaaaaa atcaaaaaac agtggtctat ggtattagtt tactcatttt
     8041 atctattatt ccattttttc ttttaacatt attgtgggta attcctggag ttttaggtat
     8101 tattgcggga ttgatgcttc taataagaaa atcttaatta ggcaatgtgt agacaacgtg
     8161 aaacgattaa tcaattaaaa gctaaatacg ttttgagaaa tgataacttg tagtcgatgg
     8221 aagacatgta gataaaaaaa ctacgtgtct tttatttatt ctaatttttt cgaaaaaaag
     8281 acactctctg attgttgtat taatggaaaa ggggaaagca gcaagtaaat ttagtttttc
     8341 tatt
//
LOCUS       DAZKLA010000119         8268 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000119 DAZKLA010000000
VERSION     DAZKLA010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.121"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            161..1033
                     /locus_tag="ACG85N_002353"
     CDS             161..1033
                     /locus_tag="ACG85N_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0225074.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            1030..2988
                     /gene="nagE"
                     /locus_tag="ACG85N_002354"
     CDS             1030..2988
                     /gene="nagE"
                     /locus_tag="ACG85N_002354"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFQ0225075.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(3320..4171)
                     /locus_tag="ACG85N_002355"
     CDS             complement(3320..4171)
                     /locus_tag="ACG85N_002355"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0225076.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWVLKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            4294..4761
                     /locus_tag="ACG85N_002356"
     CDS             4294..4761
                     /locus_tag="ACG85N_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFQ0225077.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(5024..5506)
                     /locus_tag="ACG85N_002357"
     CDS             complement(5024..5506)
                     /locus_tag="ACG85N_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0225078.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(5606..6469)
                     /locus_tag="ACG85N_002358"
     CDS             complement(5606..6469)
                     /locus_tag="ACG85N_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0225079.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            7067..7942
                     /locus_tag="ACG85N_002359"
     CDS             7067..7942
                     /locus_tag="ACG85N_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFQ0225080.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(8087..8239)
                     /locus_tag="ACG85N_002360"
                     /pseudo
     CDS             complement(8087..8239)
                     /locus_tag="ACG85N_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
BASE COUNT         2554 a         1674 c         1481 g         2559 t
ORIGIN      
        1 acgttaacaa taattcgttt catgtgacta gatcatacta ggcatgaaaa atctctgagg
       61 aacatggttt ccctaggagg tttttcgtgt ctatttttgt ttggccgcaa tcaactggtc
      121 tagcaaacgt gaaaaccaaa tcttcaaaaa gggtgcgcgc atgaaaatca aaaagatctt
      181 taatcaaaat gcagtattgg tagatgacgg tggacaggaa aaagtagcta ttggaaaagg
      241 gattggattt gacagaaaaa gaaatgattt gatttttgat cgtgatgtcg aacggatatt
      301 tgtcatggaa ccagaaggtc aaatcaagtt gcaaaattta cttaatcaga ttgatgaacg
      361 ctttttattt gcagcagaaa cgatcattga tcatgcggaa actgttttga tggaaaagtt
      421 gaatgaacat gtattgatcg ctttgaccga ccacattgct ttttctgcgg agaacattaa
      481 taacggtatc gtcatccgca acaaactttt aagagaaatc gaagtgcttt acagtgatga
      541 attcagtatt gctcaatggg cagtagagta tctgacaaaa gagcttggga ttccatatac
      601 ttatgacgaa gcaggatata tcgcaatcca tattcacagc ggacgaagcg gtcggaacaa
      661 taaccatcgc agtatccgtg aagtgacgat tgtatctgac atcatccatt tagtggaatc
      721 agaattggaa acagatatcc attcagaacc attttcactg aattattcaa gactggtgaa
      781 tcatttacga ctgcttctac aacgaaccca tgcgcaacaa tatgctgttt tagacacgga
      841 aatcgtagaa atggtcaaac gaaaataccc agaaagctat aagatttcta aaaaaatccg
      901 tgtgttgctg accaaagaat accagctcgc gatcacgaaa gaagaactgg gttacttggc
      961 catccatatc gagcgtttaa gaagcgcaat cgtacaaaca aatgttaaca atcatgagga
     1021 ggaaaaaaat tgaaagcata tctacaacga atgggacgtt cccttcaact tccagttgcg
     1081 gtcttacctg ctgctgcttt actagtaggg atcggaaact ggtgggcatc ttatagtaac
     1141 gacattgtcg ctcatttttt acaagctggc ggtaacgcag ttttaggtca attacctttg
     1201 ctgtttgccg ttgggttagc actagggatg tcagtggaca aatcaggtgc tgctgccttg
     1261 gcaggtttgg tcgcatttga agttccagta aatgttttaa aaactgattc agtagctact
     1321 ttattgaata tcaaaccaga atcagttgat ccatcttttg ctcagatcag taacgtattt
     1381 attggtatta ttgccggatt gattgcggca gctctttata atcgtttcca cgaagcaaaa
     1441 ttaccgatgg ctttatcttt ctttagcggc aagcgtctag tgccaatctt ggctgccttt
     1501 gtcatgttgc tgatcagtgc agtgctactg ctcgtatggc ctccggttta ctctgcttta
     1561 gtttcttttg ctaaattcat tttgagttta ggctggggtg gtgcaggtct ttatggcttc
     1621 ttcaaccgtt tactgatccc tacaggattg catcaagcaa tgaactccgt gttctggttt
     1681 gacgtagcag gaatcaatga tattggtaaa tttttggctt ctgaaggaac aaaaggcgta
     1741 acaggaatgt atcaagctgg atttttccca atcatgatgt tcgggcttcc agcaggagca
     1801 tatgctattt acagaaatgc acgtccagaa aacaaagcaa aaacagcttc cttgatgtta
     1861 gcagcagctt ttgcttcatt ctttactggt gtaacagaac cacttgaatt ctcatttatg
     1921 ttcgttgctt ggccattata tgtcttgcat gcaattttca ctgggatttc tttagcagtg
     1981 agtgcattct tccattggac agcaggattt gcctttagtg ccggatttgt cgattacttc
     2041 ctatctttga aaaatccgat tgcgaatcag ccattgatgt tgatcgtcca aggattagta
     2101 acagcagtga tttactactt tggctttaac tttgctatca aaaaattcca tctgatgact
     2161 cctggccgcg aggaagcaga tctttcagat gacgatactg caacgactaa ttcagacaat
     2221 aaatatgcgg cacaagcaag caaaatctac gctgcattag gcggagccga caatgtaact
     2281 tcgatcgata actgtactac acgccttcgt ttgcaagtca aagatacagg cctgatcgat
     2341 caaaataaaa tcaaagccac tggcgtacct ggtatgaaga tcatcgacgg aaaaaatgcg
     2401 caagtcatcg taggtacaga agtacaattc gttgcagatg agatggcaaa actacatggt
     2461 ggtgctgcaa cacgacctgc acaaacaaac accgtggtta aaacagaaac atttgccggt
     2521 ggaacgatcg aacaagatat ttacgctatc gcaaatggga aactgattcc aatcactgaa
     2581 gtttcagatg atgtattcgc ggaaaaaatg atgggtgacg gctatgctgt attaccagaa
     2641 aatggagaaa tcttctctcc aatcgctggt acgatcacaa acattttccc aacgaaacat
     2701 gctgtcggaa tccaaacaga tgctggtatc gaagtcttgc ttcatatggg catcaacact
     2761 gttgacttaa aaggcgaacc attcacttta tatgtaaaag aaggtcaaaa agtcgcaaga
     2821 ggtcaattga ttgctttagt tgatcttgcc gctattcaat ctgcaggcaa aaacacggat
     2881 atggtcgttg tattcacaaa tggcgacaaa gtccaaagct tagaaatcga acccgcacgt
     2941 gatgtaaaag caaatgataa aatcggctct gtttccaaca aagcctgagc catcgtcttc
     3001 cccagacttt tttatcgata aacaaacagg agctgagaca aaaatcttgt ctcagctcct
     3061 ttttccgaat aaaaacggcg ttctcctgcc aactccgcgg taataagtcc agccaaacgt
     3121 aacatatgaa gagctatttt ttatttgact cttcttatta cttgatgcag aacagctttt
     3181 ttacaacctc tgataaacgg tgttcaaaaa ccagtttatc ggtatgaagt caaatttatg
     3241 caaaaatgtg gaaagccatt ttcacaaaat ttttcttcat actcaaaact aatcggcttt
     3301 ttcacaacct ctttatacat tattcctgat ccaatatttc ttttaatgct gcttcaagag
     3361 aagttaatgg ttttcccaat actttttcta aatcatcaga ggaaatagct aactgatcct
     3421 ttttaatatc atgttggatc gctaaaaaca tgtcagctgc ttcttgaggg aatccggctt
     3481 cttttagatt ttcgacaaac gctgcatcac ttgcttcaat aatttctaac tttttacctg
     3541 tcgcacgctc taatgccttg gctaattctt catagtcagt tagattgccg gataattcta
     3601 agatttctgg agagtcactt tctactagta cttttgctgc agcttctgca tattcacgtt
     3661 tcaataccca acctgttttt ccatttcctg cagcataaac gaattgacca gtttttaaag
     3721 cctgcccaat gataggtaat tcattctcta cataccaatt attccgtaaa aatgtatggg
     3781 aaatacctga atccttaatc attttttcag tgaattggtg atctggcgct aatacactga
     3841 tcgagtgatc ggcatctgca aaacttgtat aagcgatata agatacccca gctgcttgtg
     3901 ctgccttcac tacattctta tgttcggctt gacgatttcc tggaacgcca gagacaaaaa
     3961 gcaatcgatc tattcctttc aacgcttctt tcattgagtc taaatcatca taatcaccaa
     4021 tacgtatatt aaatccagct tctttcagtg cttctccttt ttcttcacta cgtactagta
     4081 catagatttc attttgtgga accatctttt ttaagaattc cagcgcataa ccaccaaaac
     4141 ctccagttgc tcctgttact aaataatgca tatttgccac tccctattct ttttatttgt
     4201 taagatttac agctgtacat ttataaatta cacctttatc ataaagagtg tggtaagata
     4261 agtcaagcaa aaaaaatgaa aggatgacaa ctgatgaagt attctctgca atttagtgat
     4321 gcaatccatg tacttgccta tattgaaatg tttcaagata cccgatcact ttctagtgag
     4381 atgattgcca gtagtatcga aacaaatgca gcaaatgtac gtaaaatcat gagtcagctc
     4441 aaaaaatccg gtttgatctt tacaaaaacc ggaaaaccgc agccttccct tgccaaaagt
     4501 ccagatcaga tttctttact agatgtctat cgcagcatcg aaggtaatac acagcttatc
     4561 caagtcgatc caaaaacaaa tccaaattgc gtcgttggtg cgaatatcca agaagcttta
     4621 gaagaaagct acgctcggct gcagaaaaag gcagaagaag aaatggctaa gatcacactt
     4681 gatagtctga tacataaaat agccgttttg gaaaaagaaa aaagaccaga aaacacaggc
     4741 ctcgtagaga gatttttata attctctcta ctgtgcctgc atttttctga tcggaatcgt
     4801 ctacataaat tcttttattc tccgggggac aaaagccttg tcatgcccct ttttcctctc
     4861 taatgctcag gtcaaagcgc tgaagtcatg acgtcttgat aaacggtgtt caagtagctg
     4921 accctcggca ttagttctga aaaatgcaaa atatggaaag ctatttccca tttttcctct
     4981 ctaatgctca ggtcaaagcg ctactttcac gacctctata ttatcatggt ttattgacta
     5041 ccggtatcca gatttctccc atatatttcc cgttggtaac tcccatttcg actacagcat
     5101 ttggaccccc aacataggcg taatcttcta cttctcttaa aacatgccca aaggccgtat
     5161 tcgtcaaatc atttcttaat tcttcaggcg attctgctaa agcgattaca acaagatacg
     5221 ttccttttgg gaatgggatg attcgggtag tgtttggaac atcctgactc gtttgaacac
     5281 cgacatagta catcattttc ccgttatagg gttcgttgac tgtaaagaaa taatgatttt
     5341 cagcagcttg tttcaatgca tctattgttc cattaccgat aactttttgc tcaaatgctt
     5401 gtttctctgc agccaaaccg acaaaatccg tattgctact cttcaattcc acaccaatac
     5461 ccgtcaccgt aaaatctggt ttttcttcga tcctatactt tgccatttct cccacccttt
     5521 ctattatatt aagttcatct tacataaaat taaagggaaa tgacacgatt acgctctttt
     5581 ttctagggat atttttattt tttaactatt tttttgcttt cactgcaatc caaatctcac
     5641 tgtaaacatt actgaaatcc tctccccatt ttgtaaaaga gatctcggga gcttcgacta
     5701 actcataatc tgaagaagga agccattctg catagatttt cccccaagta tcttgtaaag
     5761 tttggggaaa tggcccttta ttaggaaaaa tggcccaagt gtgttcttct atagcaagca
     5821 tttctaaatc agcgtacgga ttttcttttg tcgtagcaaa gccaatcatg tgcatcaaac
     5881 tgcccttttc ttccaagcgg gctccatcaa atccataaga aacgttgatt acttgatttg
     5941 gatataaatc tcctaactta tgcatctctt ccctttgagt ctccgtgatc gtctgcgcta
     6001 acttttgtat ttcttgattg actccttcaa atttgacagg aacttttttc gccacacctg
     6061 ccacataaaa ctgttctttc ttttcgatac gaaattccat cgattttcct cctctgacat
     6121 caataaaaaa tgtcaagcgt ggaaaagatt tttgtatgcc gtatctgctg atttctgaag
     6181 gcagatatcc tgaccaatca cgaaaagctc ttgaaaaacc ttcgactgtt tcatatccat
     6241 atctaaacgc cgtttctgtc actcttgatc catctaggag atcgcgatta gccaaagcca
     6301 atctacggtt tttgatatag tccattagcg acatccctgc aataaacgta aagatgcgtt
     6361 ttaattgata ttcagagatt cctgtatttt cggctattat tttataggaa agtccttctg
     6421 tcaaatgttc ttcgatgtaa tcgatcaatt ggttcagatc tttaagcata tttatcttcc
     6481 tccttgcttg cctttttatc atattatcaa agaaagtcaa ttacttgatg aatcaggaac
     6541 caatttagtc agatgtagaa aatgttgtat ggatctaaag aaaattctca agcagcgttt
     6601 cgtaacggat aattggtatt atctgcctat ctatgtttta cctttagaaa aataaatttt
     6661 ggggaagaca cattctaagt aaacaaagcg gtaaaaatat cttttattcc cttagttaat
     6721 ccgcttacta cctactgtac aaagcaaatt gttgttcaaa ctgctgatca gaacgctgct
     6781 tggatttttc tttgcgatac atcatacctc ttttatcaat tggaacaatt atttatttct
     6841 aacaaaatca cttcattttt gtcagataaa taaaaaacaa aaaaagttcc atctttaaaa
     6901 cacgctattc ttttactttt tctcacattc tctaagaaaa gaaaaggaga atgaattttt
     6961 gacaattcga gcaaaagtga ttatcaaata tgtaaaatca gctacttttt tcttaaacgt
     7021 ttctttatac taaatataaa taagtgtttc atgaaaggag aactagatga ataattggct
     7081 tgagtatttt cctgaaaacg ttttggaacg cggttacagc tatcatctgc acggttttgt
     7141 ccgtcatttg aactatacaa gcaagtactt gtccgcaaca gtttctggca cggaagatta
     7201 taaggtagtg atcacttggg atgaaaagac caacatgact tgtgattgtc tatatgccat
     7261 agaaggaaaa aaatgcaaac acatggctgc tgtcttattc gcttacgaag aacgtccaat
     7321 aaaaaaatcc aactactcac tttccgaact ctcgtccctt gtttcctccg ctagttcctc
     7381 tcttgtacgg gagcttttaa cagagattct tatagaacat ccgcaattta tcgaacgctt
     7441 caaagttaag atgccatttc atgcaataaa ttattcagat aaattaacaa ctatcatcca
     7501 taaatacgat catattatca agaaaaacaa aaacaggaaa acagcgaagt tcatcatgga
     7561 gatgcgaaag ttcatccaag aagcagttga atcgctcatt cagcaaaatg cctatcttcc
     7621 cgcattcgaa ttgatcaatg aagtgattgc tactctcgaa acattctatt gggagcctga
     7681 ggatgaacga acccttcttc tgatagagga ctgttattat ttatggaaag aattattagc
     7741 agaagcacct catgcagaaa aaaggcaaat gttctcttgg ttcgtctgcc aagtcgatca
     7801 tactgacgcc tcttattcaa aacgctactc cataaaaatc ctgaaagaag actttcggga
     7861 aaaagaattt tctaaccaaa agaaaaaaat agataaaaaa actaaagaaa cggtaaagaa
     7921 agacgatttc aatgaaaaat agcaagtaaa cgataacaac catcctaaat ccacaagaaa
     7981 tacagcagac cttgctttaa aaacacgaat catgcaaagg atgaagtaaa gaagacttaa
     8041 cgcgtaccca atattagtca tttgactatt aggtacgcgt tttgttttat aagtcatgac
     8101 aaatttcctt cgttgctggg tagattttcc ggcattgttg atagatttta tgatactttt
     8161 ctacattttg taagagaggt tccactgcat ctgtatattg gataaaattc tgacaacaac
     8221 tttcaggtgt gtcgaaccac tctaatcttt aaagataaat attaaaat
//
LOCUS       DAZKLA010000120         8222 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000120 DAZKLA010000000
VERSION     DAZKLA010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.122"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACG85N_002361"
     CDS             476..1339
                     /locus_tag="ACG85N_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0225081.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACG85N_002362"
     CDS             complement(1468..3090)
                     /locus_tag="ACG85N_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0225082.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACG85N_002363"
     CDS             3339..3860
                     /locus_tag="ACG85N_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0225083.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG85N_002364"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG85N_002364"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFQ0225084.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACG85N_002365"
     CDS             5941..6759
                     /locus_tag="ACG85N_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFQ0225085.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACG85N_002366"
     CDS             complement(6862..7059)
                     /locus_tag="ACG85N_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225086.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACG85N_002367"
     CDS             7228..7806
                     /locus_tag="ACG85N_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0225087.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACG85N_002368"
     CDS             7901..8038
                     /locus_tag="ACG85N_002368"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225088.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2468 a         1481 c         1620 g         2653 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aa
//
LOCUS       DAZKLA010000121         8172 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000121 DAZKLA010000000
VERSION     DAZKLA010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.123"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(31..588)
                     /gene="frr"
                     /locus_tag="ACG85N_002369"
     CDS             complement(31..588)
                     /gene="frr"
                     /locus_tag="ACG85N_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFQ0225089.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(591..1313)
                     /gene="pyrH"
                     /locus_tag="ACG85N_002370"
     CDS             complement(591..1313)
                     /gene="pyrH"
                     /locus_tag="ACG85N_002370"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFQ0225090.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1449..2330)
                     /gene="tsf"
                     /locus_tag="ACG85N_002371"
     CDS             complement(1449..2330)
                     /gene="tsf"
                     /locus_tag="ACG85N_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFQ0225091.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2429..3211)
                     /gene="rpsB"
                     /locus_tag="ACG85N_002372"
     CDS             complement(2429..3211)
                     /gene="rpsB"
                     /locus_tag="ACG85N_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFQ0225092.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3570..4049
                     /locus_tag="ACG85N_002373"
     CDS             3570..4049
                     /locus_tag="ACG85N_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0225093.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4266..5357
                     /gene="phnW"
                     /locus_tag="ACG85N_002374"
     CDS             4266..5357
                     /gene="phnW"
                     /locus_tag="ACG85N_002374"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFQ0225094.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5350..5478
                     /locus_tag="ACG85N_002375"
     CDS             5350..5478
                     /locus_tag="ACG85N_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFQ0225095.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5482..6027
                     /locus_tag="ACG85N_002376"
     CDS             5482..6027
                     /locus_tag="ACG85N_002376"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFQ0225096.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6481..8172)
                     /gene="argS"
                     /locus_tag="ACG85N_002377"
     CDS             complement(6481..8172)
                     /gene="argS"
                     /locus_tag="ACG85N_002377"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFQ0225097.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
BASE COUNT         2242 a         1676 c         1503 g         2751 t
ORIGIN      
        1 atgatccgcc cctctgtttt tatgatttta ttaaacttcc aataattctt tttctttatc
       61 agcagcgatc ttatctactt ctttgatgct gttatctgtc aatgtttgta cgtctttttc
      121 taatccgcgc aaatcatctt cggtaatatc accatttttt tgttgttttt tatattcatc
      181 gatcgcatca cgacggatgt tacgaatcgc gatttttgca ttttctgctt cttttttcac
      241 atctttagct aattcttttc tgcgttcttc tgtcagttgc gggataacta agcgaatgac
      301 atttccatca ttggttggac tgattccgat atcacttgcc ataatagctt tttcaacatc
      361 ttgaagaatg ctcttgtcaa aaggtgtgat cattaaaaca cgtgcttctg gaatttggat
      421 tgatgccatt tggttcaatg gtgttggcgc accgtagtag ttcacagtaa tacggtccaa
      481 aaggctagcg ttcgcacggc ctgcgcggat ttgcccaagt tcacgttgaa gattttgtgc
      541 tgcttttttc attttttctt ttgcttctgt cataattgca tctgccattt ttatttcccc
      601 cttacagttg ttccgatatt ttcgcctaag attgcacggc gaatatttcc gtgttcattt
      661 aaattgaaga caagcagtgg aatatcatta tccatgctta gtgaacttgc agttgaatcc
      721 atgacttgta atcctttggc aataacttcc agatgagtca gttcttcaaa ttttactgca
      781 tttgcatcta ctttcggatc agcagaataa acaccgtcta cattgttttt agccatcagg
      841 ataacatctg cgccgatttc tgccgcgcgt aatgctgctg ttgtgtctgt cgagaagtac
      901 gggttgccgg taccgcctgc aaagataacg atccgtcctt tttctaaatg acgttctgct
      961 ttacggcgaa tatatggctc tgcgatttga cgcatttcaa tcgaagtttg cacgcgtgtc
     1021 ggtacaccca cgttttccaa cgtatcctgt aaagctaagg cattcataac tgtagctaac
     1081 atacccatgt aatcagcttg agcacgttcc atgcccattt gtgctccgat ttgtccgcgc
     1141 cagatatttc cgccgcccac aacgattgcc atttcaacgc ccagctcgtg aacttctttg
     1201 atttcttgga tgatttcttt gattactggt ggtttgattc caaatcctgc gtccccagct
     1261 aaagcttctc cactcaattt taagactaca cgctgatatt tcggtgtgac catgttcatt
     1321 cctccaatat tttctattgc cattttacca tacttagatg atttcgacac tctaagactg
     1381 gtttttcttg ttttgcttaa aaaaagggag cgcacaaacc gtgcgttccc gtctgttatt
     1441 caacagaatt attttttcac ttggctcata acttcttcta caaagttatc ttcgcgtttt
     1501 tcgatacctt cgccaacttc gaaacgtacg aatgttttaa cagttgcgcc ttttgatgca
     1561 acgtattttt caacagtcat atcaggatct ttaacgaatg gttggtccac caaagcgatt
     1621 tctgctttga atttcttcaa gcgtccttct accatttttt caacgatatt cgctggtttg
     1681 ccttcgttca atgcttgttc tgtaagaaca gttttttcat gttctaattc agcttctgga
     1741 atttgtgatt cgtttacata acgaggattg atagctgcta cgtgcatagc tacgtctctt
     1801 gcaactgttt cgtcagttgt tccatctaga acagtcaata cggcaatacg tccgcccata
     1861 tgcaagtatc caccaaatgc agcattgtcg tctttttcta caacttcaaa gcgacggaag
     1921 ctgatttttt ctccgataac ttgagttgct tcgatcaagt cagattcaat tgtaccttta
     1981 tctgttttga ttttcattgc agcttccatg tctgctggtt tgttttcagc aacaagttct
     2041 gcaatttctt ttactaaatc ttggaacatt tcgtttttag aaacaaagtc tgtttctgag
     2101 tttacttcaa cgattgctgc agtgttacct ttcacagcaa ctgaagccaa tccttcagct
     2161 gcaatgcggt catttttctt cgcagccttt gccattcctt tttcacgtaa aagatcaact
     2221 gctttttcca tgtcgccttc tacttcaact aatgcttttt tcgcatccat cattcctacg
     2281 ccagtcattt cgcgtagttc ttttaccatt ttagctgtaa cgtctgccat gtttaactcc
     2341 tcctgtgtgt ttttcttttt aaaaaaagct gcttcaaagg agagtgaggc ttgtcgccca
     2401 gcctttgaaa cagctccatc attcaaaatt attctgctga agaattgtcg ccttcaacaa
     2461 catctacgat ttcttcgatt gatgttgctg tgttttcttc tgtgaaatct tcttcaacta
     2521 cttgatcttc accttggttt ccttcgatga aagcatcagc catttttgaa gtaatcaatt
     2581 taacggcacg aattgcatcg tcgtttgaag ggattactac gtcgatttca tctggatcgc
     2641 agtttgtatc aaccatagct acgattggga tatttaattt gtgtgcttct tgaacagcaa
     2701 tacgttcttt acgtgggtca acgatgtaca ttacatctgg gattcttggc atatcagcga
     2761 taccgcctaa gaatttttca agacgttcac gttctttgtt caaaccagca acttcttttt
     2821 taggtagaac ttcaaaagtt ccatcttctt ccattgcatt gatttgtttc aaacggctga
     2881 tacgtttttg gatagtatcc cagtttgtca atgttccgcc taaccaacga tggttaacga
     2941 agtattgtcc tgcacgagtt gcttcttctt tgattgcttc ttgtgcttgt ttttttgtac
     3001 ctacgaataa tgctacgccg ccttcttcag caacgctctt catgtaatca taagctgcat
     3061 ctactaattt aactgttttt tgcaagtcaa tgatgtagat tccgtttctt tctgtgaaga
     3121 tatatttctt catttttgga ttccagcgac gagtttggtg accaaagtgt acgccggctt
     3181 ctagtaattg tttcatagaa attactgcca tgtttgtttc ctccgatttg gttttatttt
     3241 tcctcttctt atcttctacc ttgccggcaa actttttcaa gcacctaccg acaatccaat
     3301 aagaatgtgg atttgctgat gcataaagca ccctcagcta ttatacagac aaaagatccg
     3361 tatcgcaaga gcaaatatac ttttctgctc aacattcaat agtttacctg aaagataaaa
     3421 agaattcaag aaaaacagta gatttctctt tgtttcttta ttatcctttt tccataaaat
     3481 aattttaggt ataaaagacg aatattattg ggcaatcctt cttttttttg ttatgataca
     3541 tgtgaaatct ttcgtaaagg agaaagaaaa tgcgcaaagc tcaaagacat ttgctgatta
     3601 aacaagtcat tgaagaattc ccgatccgta cgcaagaaga actattaaac aaacttgggc
     3661 attatggcgt taccgccacg caggcaacga tttctcgaga tatccgtgat ctaaaaatcg
     3721 tgaaggcacc tgatgaaaat ggcgtttccc gcttcgtatt gtttcgcgaa aaaggaactc
     3781 ctgagactag gactgaggaa gaggatcggc tggttcaaat gattgaagac attgtattga
     3841 aagtcgaacg agtccacttt ttgacgatcg tcaatacctt gccagacaat gctcatttat
     3901 ttgcatctgt tttagatgag atcaaacctc cctctattgt cagtacaatt gctggattcg
     3961 atacgatcat ggtcatctcg gaaaatgagg aggacgcaaa acgagtagaa gaatttcttc
     4021 accatccaac tgaagtatca cttttttaat cagagcacgt attcctaata tggaatgcgt
     4081 gtttttttat tttcaatatt ttccttattg aataaacctt tacactcttt acaaaaatac
     4141 atcatttttt attcaaattt ttcaacacct tgattgaatc acaataatca tcatttatat
     4201 tggatgttaa caagagcagc ttcttgctct ttgtgtattt caaaaaagaa atgaaaggaa
     4261 atcatatgta taaattatta acttctggac cattgacgac ttctgaaaca gtgaaaaatg
     4321 ctatgctgat cgatcattgt acttgggatc aagaatacaa agaaattaca caatggataa
     4381 gaaaaaaatt attggatctt gctcaagtat cggaaggaat atacacaacg gtcttaatgc
     4441 aaggtagcag ctccttcggg gtagaatcgg tattaagttc tgtgatcagg ccagaagata
     4501 ctgtactgat ttgttcaaac ggcgcatata gacaacgaat gatcaacatg tgcgaatgtc
     4561 tgaaactgaa ctataccgtt tatgaggtat cggaacatca aatcctccaa gctcatgtga
     4621 ttacacagct gattgaagag aatccgtcta tctccgcact agcaatggtc catagcgaga
     4681 ctacttccgg tattttgaat aatttggaag agattgctgc tgtcacacaa gaaagagatc
     4741 ttttgtttat agtggacgcg atgtcaagct ttggtgggat accgatttgt gtagccgaac
     4801 tagggattga ttttttgatt tccagctcaa acaaatgtat tcaaggtgtt ccaggctttt
     4861 cttttgtact tgctaataaa gcggaactgg aaaaacgaaa aaatgttagc cgaagtttgt
     4921 cacttgatct atatgaccag taccgcgtga tggaaaacga gaatggcaaa tggcgtttca
     4981 cttcccctac ccatacggtt ttggcttttc aacaagcgct gaaggaacta gaggcagaag
     5041 gaggaatcga aactcgatat tcacgttatc tcgccaataa tcatgtgcta cgacaaaaaa
     5101 tgacagagat ggggttccag cctttcattc aagaatatca ggggccattc atcacctctt
     5161 ttctttaccc taaggactgg cgatttgatt ttaatcattt ttatcagtat ttaaagcaaa
     5221 atggatttgt gatctatccc gggaaaatca gcgatcatga atgttttcgt atcggaaata
     5281 ttggcgagat ctatccggaa gatattttgg aactgacagc aattatcaaa gactataagg
     5341 agaacgttaa tgattaaaac tgtaattttt gattgggcag gaacaactgt ggactttggc
     5401 tgtatggctc ctgtccacgc ttttagaaat gcttttctag aaaaaggaac ccaactgact
     5461 gacaaagaaa tccgctaacc gatgggaaaa ttaaaatggg atcatatcca acagcttctt
     5521 tccctccctt ctgtcaaaaa acaatggata gctatttatg gacaactacc tcaggaaaaa
     5581 gaggtggagg aattgtatca acaatttgaa cattatctgt ttaaagattt ggtcaagcat
     5641 tctaccttga aacccgacac attggaaacg atcgaacggt tgagaaacgc tgggatcaat
     5701 attggttcca cgaatggatt taatgctcct atgatgacaa tcgtagcaga aaccgcaaaa
     5761 aaagaaaggt attcaccaga cttcatggca acttttgaag acgtcgaagg gtatggtcga
     5821 ccgtttcctt atatgatcca taaaaacatg cagtacttca aaaacaagtc ggttgatgaa
     5881 gtaatcaaag tcggagatac ggtctcagat atccaggaag ggaaaaatgc cggagtgttg
     5941 acagtaggtg taattgaagg tagttcgcta atggggattt cttatgatga gtaccaaaaa
     6001 ttagagatgg ggaaaaagat cgtttaaaac agtctgttgc tcttagattc caacaagccg
     6061 gcgctgatat ttgtgtaaac aatttaaaag agttaacaga atacattttg tacgctgaca
     6121 agaatccaga atgactttta tacaaaataa gaggttgtga aaaagctgtc ctgtatcaag
     6181 taataaaaac agccaagcaa aaatagctcc tcatattttt cgtttggtta ggcttattac
     6241 cgcagatgca agcttttgaa caccgtttat tcgaaaaaaa aaagggagct gtgacacgac
     6301 ttttgtcaca gtccccttat ttgcaaacaa acagtgttta agagacaacc tctccactaa
     6361 acggtgttca ttcagctgac tctcggtatt tgctcaaaaa acgataaaat ttgaaaagct
     6421 attttctctt ttgtttctct aatactcgga tcagaacgct gatttcacat tctctttttc
     6481 ttacatctta tctggtgcat gaagtcctag caaacgaagg tcttctttca ataatacagt
     6541 cactgcataa actagagcta agcgtgcttc tttttgttca tcatcagcta aaatcttcgt
     6601 atgagcatag tatttgttga atgcttgtgc taatttgatt gcatgtttcg caatcactga
     6661 cggttcgtat ttttccccag cagacaagac cgtttcaggg tatttttgaa ctaatttaac
     6721 cacttcccag ctggtatcgt cattcaatgc atagtctgct gtttctgaag ggacaaatcc
     6781 tgctttttcc aacaggctga ctgcacgagc atgtgtatat tgcacataag gtccagtttc
     6841 tccttcaaat cgaacaactt cttccaggtt aaagtcaaat gtattcagtc gatcgttttt
     6901 caaatcgtgg aaaatgactg ccccaacccc aacttgttta gctacagcat ctttgttttc
     6961 caaatcaggg tttttctcgc tgatttgttc tttagcagat tggattgctt catttaatac
     7021 ttcttctaat aagacgatct ttcctttacg agttgataat tttttaccac cttgcgtgat
     7081 caaaccaaat ggaatatggt gcatgtcgtc agaccagtca aagcccattt ccttcaagac
     7141 agctttcaac tgtttaaaat gatagctttg ttcattccct actacatata gagattgttt
     7201 gaagtcgtaa gtacgtttac ggtacaatgc agcagctaag tcacgtgtaa tgtatagtgt
     7261 cgcaccgtct gacttcttaa tcaacgctgg attcaggtca taagcagata ggtcaacgat
     7321 ctctgcacct ttatcctcgt tcagcaaatg tttttcttct aaaagtttga cgatttcatc
     7381 catcttgtca ttgtagaaag cttcaccgtt caacgaatcg aaacgtactt ccagcagatc
     7441 atagatttta ttgaattctt tcatcgattc atcacggaac cattgccata attgaatagc
     7501 ctctttatcg ccttcttcca gacgtttgaa ccaagcacga gcttcttcat ttaattctgg
     7561 ttctgtttca gctacttcat ggaattggac gtataaacga agcaattcat tgattggttc
     7621 tgctttaaca gcttcttctg taccccattt tttgtaagcg acgatcagtt ttccaaactg
     7681 tgttccccag tcacctaaat ggttgatgcg gattggctga taaccgattt tttccataat
     7741 gaaaccgatc gaattaccga taactgtcga acgcaaatgt cccattgaga ttggtttggc
     7801 aatattagga gaagacatgt cgattggtac tgttccttga ttcccgatgt tgctatcccc
     7861 ataatgttct ttttctttca ctactgtttg caaaactttt ttgctgataa gttctttatt
     7921 catgaaaaag ttcaagtaag gtccgacaac ttcgattttt tcaaagttgg cagaatcgat
     7981 tttttctgct aaatctgcag cgatttgctg tggcgctttt cggtaaactt ttgctaatga
     8041 aaaggctgga aatgcaacat ctccgtgttc agctgatttt ggattttcta gtaattgctc
     8101 gacttgttcc agtgttaaat catctttgac agcgtcatag acagcttttg cgacaagatc
     8161 tttattattc at
//
LOCUS       DAZKLA010000122         8056 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000122 DAZKLA010000000
VERSION     DAZKLA010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.124"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..589)
                     /locus_tag="ACG85N_002378"
                     /pseudo
     CDS             complement(133..589)
                     /locus_tag="ACG85N_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            754..3116
                     /locus_tag="ACG85N_002379"
                     /pseudo
     CDS             754..3116
                     /locus_tag="ACG85N_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="ACG85N_002380"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="ACG85N_002380"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFQ0225098.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="ACG85N_002381"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="ACG85N_002381"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFQ0225099.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="ACG85N_002382"
     CDS             5704..6489
                     /locus_tag="ACG85N_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0225100.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="ACG85N_002383"
     CDS             6502..7353
                     /locus_tag="ACG85N_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0225101.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="ACG85N_002384"
     CDS             complement(7477..7929)
                     /locus_tag="ACG85N_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0225102.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         2708 a         1371 c         1709 g         2268 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttc
//
LOCUS       DAZKLA010000123         7989 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000123 DAZKLA010000000
VERSION     DAZKLA010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.125"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            69..770
                     /locus_tag="ACG85N_002385"
     CDS             69..770
                     /locus_tag="ACG85N_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0225103.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1379..2395)
                     /gene="tsaD"
                     /locus_tag="ACG85N_002386"
     CDS             complement(1379..2395)
                     /gene="tsaD"
                     /locus_tag="ACG85N_002386"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFQ0225104.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2392..2856)
                     /gene="rimI"
                     /locus_tag="ACG85N_002387"
     CDS             complement(2392..2856)
                     /gene="rimI"
                     /locus_tag="ACG85N_002387"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0225105.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2863..3405)
                     /gene="rimI"
                     /locus_tag="ACG85N_002388"
     CDS             complement(2863..3405)
                     /gene="rimI"
                     /locus_tag="ACG85N_002388"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0225106.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3389..4111)
                     /gene="tsaB"
                     /locus_tag="ACG85N_002389"
     CDS             complement(3389..4111)
                     /gene="tsaB"
                     /locus_tag="ACG85N_002389"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFQ0225107.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4302..5282)
                     /locus_tag="ACG85N_002390"
     CDS             complement(4302..5282)
                     /locus_tag="ACG85N_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0225108.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5306..6790)
                     /gene="galT"
                     /locus_tag="ACG85N_002391"
     CDS             complement(5306..6790)
                     /gene="galT"
                     /locus_tag="ACG85N_002391"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFQ0225109.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6802..7791)
                     /gene="galE"
                     /locus_tag="ACG85N_002392"
     CDS             complement(6802..7791)
                     /gene="galE"
                     /locus_tag="ACG85N_002392"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFQ0225110.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2257 a         1710 c         1407 g         2615 t
ORIGIN      
        1 taaaacttta caaattcttt aaaaaaagtg acaataaata tagtgaacaa taaggagagg
       61 actttttaat gcgtgttaat aaaatcgatt ttcacttttc aaaactcaga tatcaacctg
      121 attttgaaca tttctcggat gaagagttcc aacagatcaa ggaacatact gttcttcgtt
      181 cttataaaaa aggacagatc cttttcgacg atggtgatcc cagacaacgg ttgtatatct
      241 tgaccaaagg actcgttcgt ttagaacgtt atgatgaaag tgcaacttat tactactatg
      301 actatgtgaa tacaaataac ctttttccta tgtcaggaat gtttggagaa gagacctaca
      361 cttatacagc tcaagcgatg actgatattg aaacattcta ccttcctgtt tcctactatg
      421 aaaaattagc acgaaaaaat cccgaacagt tgatctatat gatacaaaaa atttctaaag
      481 tagttgaaat acatgaattg cgtatccaaa tcggattgac ctctagcgcc tttgaccggg
      541 taaaacaaaa tttattcatt ttgaaagaag aattgggcgt accaacaaaa gatggtcata
      601 tcaccattcc ttatccgatc accttaaaag aattagcagt gaatagcggc acgacacgtg
      661 aaactgctgg acaagtaatc aaacaactac gtgattccgg gctgttagat tatgacaaaa
      721 agcgttttac tttctgtaag acagctacta ctccagctat gagagaataa cgcgaacaaa
      781 aataagcagt gaccctgata aatatcaggg tcactgctta ttttcctccc taaaacttct
      841 gaaagatccg gcagttttag atcactggac aagactcaaa gattttctat gtgtgtttca
      901 gttatgtaat attcactgtt tgcaaaaaat actgttagat taactagatg aagcgtcttg
      961 tcctcatccg atctagatta ttcgatattc ctcgacaata aacgattact cagtgagggc
     1021 aatcagagga acggataatg ataaataagc tgtaaaaaat agcaaaataa caataatgat
     1081 taaaagtatg taaaaaatag ggctttgaca aaaacgtgtc acagccctat ttcgattaaa
     1141 cggtgtgtac ctgtcaactc ttcggtcaca agtcataatg ttcataaaca tgaggagctg
     1201 ttttttgaaa ttgttccttg tgaatcagag ttaaacgaca ggcttacgaa ctcttgatta
     1261 aacggcgttc aaaagcttgc atacgcggta ataagaacag ccaggtcaaa aacatgagaa
     1321 gccgtttttc ctgggctgtt cttattactt gatgcagggc agctttttca caacctcttt
     1381 attcagataa aaccaagctt ggatcagcat tcaactgata gctagcaaaa tttctttttt
     1441 gcatttccac atgtgctgct gctccaatca ttgcagcatt atctccacaa agtcgaagag
     1501 ggggaatcac tagttctacc tcagggagtt tagttgccaa agcatcctgc aatccttcac
     1561 gcaacccttg attggccgct acacctccag ctataatcag ttgcttgact ggataatttt
     1621 gacaagctcg aagtgtttta gtaatcaata catcgatcac acttgcttga aagctagctg
     1681 ctaaatcatt tttgtctaga tcttctccac gctgttgtgc attatgcacc aaattgataa
     1741 atgcactttt taatccgcta aaactgaaat cataattatc ttcatggatc atcgctcgcg
     1801 gaaaatggta attatctttt ccctgatgag ctagctgatc gatttctttt ccgcttgggt
     1861 aagaaagacc taatactctt ccaactttgt cataagcttc tcctgctgcg tcatctcttg
     1921 tctccccaat gatttcatat gatccatctt cttgcatata gactaattct gtatgtcctc
     1981 cgctaacaag aagtgccatc aatggaaact ggaatggttt aaccagacgg gctgcataga
     2041 tatgccctgc catatggttt acaggaataa gcggcaattg gtgtgcccaa gcaaaagcct
     2101 tagctgcaga aataccaatc agcaacgagc cgacaagtcc tggtccataa gtgacagcga
     2161 ctgccgataa atcttctgcc gacacaccag cttctaccag tgcatcttcc aagcataagg
     2221 tgatttgttc tacgtgatgg cggctagcta cttcaggtac tacgccacca aaacgtttat
     2281 gactgtttat ttgagaagca acgatgttag ataagatctc tgttccattt ctaacgaccg
     2341 ctacactcgt ttcatcacaa cttgattcaa tcgccagaat caattcttca ttcatttcat
     2401 cgtctccgtt tttaactttc tgctcataat gatcgcatct tccgtcggat tgcgataata
     2461 tgatttccgt ttacctaata tacgaaactt ttctgattca taaagttttc ttgctgcttc
     2521 attagataga cgtacttcta aaaaaacatg cagcgaatct tttatttgtt ccgtaatcag
     2581 taattgtaac aaatgccgtg catgcccttt tccttgttct gaaactgcaa cagcaatatt
     2641 tgtgatctcg acttcatcta ttactttcga gtatccaata aagccacaga tctggttatc
     2701 ttcagctaaa agcagataat cagagtgagg ctgttggatg tctgccaaaa actgcatttt
     2761 cgtccaaggt gaaccatgtg gataagcatt ttcgctaatc atccatacgc attctgccaa
     2821 gtacgcttca tcaaaatctt tttttctgca aatcaactaa tctcactcat tttctaaaag
     2881 ttttatcata tccaacgcat cttcttttgt ttcagtgtaa tatctttttt tgatcgttgt
     2941 tgcttgaaac cccaatttcc gataaagtct ttgtgcatct tgattactca tacgcacttc
     3001 taatgacatc gtctcacagc gattcattat agcaaagttc tcgatttcgt caataagcag
     3061 cgaaccgatt cgtttacgct gatatgccgg tcgaacggca atattcgtga tatggcaatc
     3121 taaaccgaac gctcttgaac cgataaaacc aatcattctt ccattatcaa agatacctag
     3181 atatagatga ggaagcggtg acttcaattc tgatataaag gcactttttg tccatggcaa
     3241 ttcgccatta tagacttctc gttcgacatc cagcagctct ttgatatctt ctgtagtcaa
     3301 ttctcttagt acgtaacgac gatcgatttg ttcgactgtt ttttctgtat aaatcatttt
     3361 atcttgaaaa aagcttctgg catacgctct aaattttttc aacataactt tcgtctccag
     3421 gtgtgtgcgt ctccaaccat ttttcttctg cttctactcg ttttaaatag cgaggaagaa
     3481 aagcttgttt ctctgcaact ggttcaacta aagcacctaa ttgagccaca gttacaccac
     3541 ttggaatatc ccatgctgga actgtattaa tcatcgcact aggcaatatt tcctttattt
     3601 cagagaagaa tttgtgacaa tcactcccga cgaaataaat agcttcattc agcttttgta
     3661 gttgtgtgaa caaatcagaa gcagaaatat gtacatcttc aagtacagtt tctaatctat
     3721 tttctttcca gcggtatgcc ccagcgtata cattttttct tcgagcatca aacatgggca
     3781 caataatatt atccacaccg atgcaattag ccgcgatggt tttcagacta gatactccta
     3841 ctagctcttt atctaatgta taagcaatcg tttttgctgt cgttacacct atacgaagcc
     3901 ctgtataaga accgggacca tcagaaaccg cgattcggtc aatgtctttt ggacttattt
     3961 gaagatctgc aaacaattgg tcgattgcag gcatcagtgt tacactgtgg ttgcgtttga
     4021 tatttgtttg tatttgtcca agaatttgtt cgtcttctac cacaccgatc gccaaagttt
     4081 ggtttgcagt atctattcct aatgtaatca tatatttgat cctttctgct atgacattct
     4141 cgccttccat tgtagcatac ttttttattc tgaggatgaa aataccaaag gcaaaactag
     4201 tacaataata catgattatc tgctggcggg caaattttgc cccttcctga aacacaaaaa
     4261 agctgcggta tcactccatt ttcccacagc ttttccatct tttatttcgt tgattctcga
     4321 ataatcaact ctgtttccac aataatcttt cttgaaaccg gtgctttgga attacttaat
     4381 tgaagaatcg tctccactgc tagctctccc atccattcgg tataaacttt tacagtagaa
     4441 agcgagggcg ttacatattt cgctacactg acatcattga agcccatcac agaaatatct
     4501 tctggtacca ctaaaccttt ttcttgaatc gctttcagtg cgccaatcgc taatgcatca
     4561 ttagctgcaa aaagcgcatc aggaacatgc ggattcgttt ttaaatactg cttcactgct
     4621 tgatatcctg actcgacaga aaaatctgct tcgataattg gaccttcgtc gtagcctttt
     4681 tctgccaaaa cttgttgaaa tgccataatt ctgggatctt ttagccgctg atggtttttc
     4741 ttcgtatatt ctgttccagc aagcatagcg atatgttgcg ctttatggga gacaaagtgt
     4801 tcaatgactt tttgaacact tgacttaaag tcgacaacta gagagtcgat tccttgagaa
     4861 gtgccgtcag aatctaccaa aagcaaggca ttttgggatc ctttcatttc taataactga
     4921 gcttcgtcga attttcctag tgcgatcgtt ccatctgccg gaagatcact aatggaatcc
     4981 cacgtttctt tcaataaagt aacgtttgct tcttctgcct ttttttcgat tcctaggcga
     5041 atagataaat aataaagatc ttcaagctct tccgtatcgt cgtaccactg gatcaggcga
     5101 agcgtttgct tagtatttgc tgcattttta cgatgtttcg tataatttaa gcgttctgct
     5161 gcttcaaaaa ttttttgctt tgtggctaag ccaacggaaa gatcaggatc atagttgaga
     5221 actcttgaaa cagtagcagg agaaactcct gccaactgcg caatgtcttt aattgttgcc
     5281 atttgtctct cctccttttt tacgtttata aaatcgcagt aaatcgttca aacgctgcct
     5341 gtcctttttc atctcgcttg aatactccag cgtcttccag tacacgtgca aaaacctgac
     5401 ctacagattt ttgtacgaca tcggtgaccg tatctttcgt caaatcagga tgttcttttt
     5461 ttaattgttc tgcccacgga cgatgatatt cagccatctg gttttcttct cctaataaga
     5521 aatgctctac ttctactagt tctggtttca aacgaggtgg taaaacagca agccccatta
     5581 cttcgatcaa cccaatgttc tcctttttga tatgttggac atctttatgt ggatgaaaaa
     5641 tcccatcagg atgttcttct gaaacattgt tgtctcgaag gacaaggtcc aactcaaagt
     5701 aattgtccct tcttcgtgca attggcgtaa tcgtatggtg tggtgtacca tctgctgaaa
     5761 aagctctgat agatactgtt tcatccgaat atgctttcca tttttccaat actgagttag
     5821 ctgcttcaat aagcaagttt ttctctgttg attgcaaacg gatcacagac atcggccatt
     5881 tcacgactcc tgcgatgact tgcggataat cttctagttt gataagtttt tctatcggtg
     5941 cttttgccat aggaaattca tgacgtcctg cctgataatg atcatgagaa agaatcgatc
     6001 ctccaacaat tggtaagtca gcattcgaac caacaaagta atgaggcaag acttcagtaa
     6061 tccgaagcag tctagcaaat gtttcctttg tgattttcat cgggcggtgt tgttctgata
     6121 aaatgatcga atgttcattg taataagcat aaggggaata ttggaatccc cagctttcac
     6181 cgtctagatt cattcgaata atccggtgat tggtgcgggc tggatagttc aagcgacctc
     6241 tatatccttc gttttccatg caaagcatgc aggtaggata gtctactttt accgcctcgc
     6301 gttctgctgc gatttgtttt ggatcttttt ctggcttaga taaattgatt gtgatttcca
     6361 gttcaccata ctccgactct gccggaaatt tgatgttttt tgcgatggct cttgttttga
     6421 tgtagtcatt tttcttactt aaattaaaga aatactcagt tgcttcctct gggcttttcg
     6481 aatagtgctg tgcaaagaat gcattgacga ctgaaggtgg cggtgtgaga aaatccatta
     6541 attgcgcttc aaaaatctct tgttctgctg gcaattctga aagaacacca ttttctcttg
     6601 ccttagcgac aagctgatcc aataagtcta cagattcttc cttaactggt cttacttcaa
     6661 tgctttctag agcatcttct cctaccatcc ctaaaagacg attctgtaaa tataagcggt
     6721 ccatttccat ccatccgcca gcttcgatcg caagtgtggc aaaatcacaa atcgtttgac
     6781 tgattgacat atctcattcc cctattcttc ataaccattt ggatggctga catgccaatc
     6841 ccatgctgtt ttgataatcg ctttgatatc tgtgtattca ggtttccagc ccagaatctc
     6901 tctcgctttt tcacttgaag cgactaatcg gctaggatct ccagctcttc ttggtgctat
     6961 ttctgctgga atttcttttc ctgtcacttc tctagctgct tctagcattt ccttgactga
     7021 gtatcctttg ctgctgccta aattgaagaa gttgctttca tttccttctt tcaagtattc
     7081 caaagctaaa atatgtgcag caatcaaatc ttctacttga acataatctc tgatacatgt
     7141 tccatctggt gtatcataat cgtctccata aacagccaag gcttttcttt gaccaagagc
     7201 tacttgtaaa atgattggga caagatgcgt ttctggtgta tgatcttctc cgatagatgc
     7261 atctgctttt gctccggcta cattgaagta gcgcaaagcg acatagcgca tgccatatgc
     7321 ttggtcacac cacttcatca ttttttccat catcaattta ctttcaccat acggattttt
     7381 aggattagct ggtgtatctt ctgtgattgg agactctttc ggctctccat aagttgctgc
     7441 agtagaagag aatacgattt tattcacgtt gaactcgtgc atgacttcaa gtaagatttg
     7501 cataccgtat acgttgttgt tgaaatacat caatggtttt tcgacagatt ctcctactaa
     7561 agagcttgca gcaaaatgga ttactccttc aatcggttcc ttttcaaata cggaacgtag
     7621 aaattcttta tcccggatgt ctccttcata aaaatgagca tctggatgta ctgcttgttt
     7681 gtgacctgtg agcaaattat ctaccacagc tacttgatac cccttttgaa ctagctggtc
     7741 tacagcgtgc gaaccgatat aaccggctcc gcctagtact aaaattgtca taaaaaacac
     7801 tcctttgatt agtaaaattg tgtttgttga ttctatataa tttaaaaaac gaagtttact
     7861 tttatggtga aaacaaacaa agtaaaattt cgtaaattca tatggtaaat ttataataaa
     7921 acatttagta aacattttca aggatttttt gcaaaggttt acaaacaaac gtaaaactag
     7981 ccttttttt
//
LOCUS       DAZKLA010000124         7790 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000124 DAZKLA010000000
VERSION     DAZKLA010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.126"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..641)
                     /locus_tag="ACG85N_002393"
     CDS             complement(<1..641)
                     /locus_tag="ACG85N_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFQ0225111.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQT"
     gene            complement(643..1407)
                     /locus_tag="ACG85N_002394"
     CDS             complement(643..1407)
                     /locus_tag="ACG85N_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFQ0225112.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1415..2092)
                     /locus_tag="ACG85N_002395"
     CDS             complement(1415..2092)
                     /locus_tag="ACG85N_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFQ0225113.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2129..3514)
                     /locus_tag="ACG85N_002396"
     CDS             complement(2129..3514)
                     /locus_tag="ACG85N_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFQ0225114.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3724..3808)
                     /gene="ffs"
                     /locus_tag="ACG85N_002397"
     ncRNA           complement(3724..3808)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG85N_002397"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="ACG85N_002398"
     CDS             complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="ACG85N_002398"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFQ0225115.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4453..5310
                     /locus_tag="ACG85N_002399"
     CDS             4453..5310
                     /locus_tag="ACG85N_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0225116.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5391..5726)
                     /locus_tag="ACG85N_002400"
     CDS             complement(5391..5726)
                     /locus_tag="ACG85N_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225117.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5793..7163)
                     /locus_tag="ACG85N_002401"
     CDS             complement(5793..7163)
                     /locus_tag="ACG85N_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0225118.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7241..7777)
                     /locus_tag="ACG85N_002402"
     CDS             complement(7241..7777)
                     /locus_tag="ACG85N_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0225119.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2213 a         1659 c         1268 g         2650 t
ORIGIN      
        1 tttgtttgtt tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat
       61 aatacaaaaa ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt
      121 ggatcagtaa cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg
      181 ataaaaaagc gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg
      241 ccgatttgaa acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata
      301 aacaaaatac tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt
      361 ttcacttcat aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg
      421 atggttttga agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc
      481 tcttgatcta aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt
      541 ggtcgaaagt tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac
      601 aatccagcat attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc
      661 cagtgattgt ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt
      721 aaaacctgat aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt
      781 ttcatagtta tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc
      841 tttatccata atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt
      901 ctctacaaaa gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt
      961 cttatctggg ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac
     1021 tactttttca taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc
     1081 ttcccatgta aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata
     1141 gacttttttc ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta
     1201 gactgtctct ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc
     1261 tggtgactta gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc
     1321 ttccacaaat cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa
     1381 atcaattaaa tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa
     1441 atgctgtttt tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg
     1501 tttttgctga ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg
     1561 gtcctttttt ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag
     1621 ttgtcttttc ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc
     1681 aatattcgta aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat
     1741 tatccgcttg gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat
     1801 aatcatatcg ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt
     1861 cacgataatc ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta
     1921 acagtgtcac tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca
     1981 aagcagctgt cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag
     2041 caatttctgt tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac
     2101 cacctatctt ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat
     2161 ctcctaccac acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa
     2221 tcgttggaaa aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat
     2281 tcagatcgat tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc
     2341 cattatagac catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt
     2401 tacgtaaaaa gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac
     2461 ccaatgtgac atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat
     2521 tgacccgccc ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg
     2581 tctttcctcg ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga
     2641 tatctagact cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc
     2701 cacgttctag ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg
     2761 tagggatggc tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga
     2821 cttcaccgac atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt
     2881 gaccttctct gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt
     2941 cttttgcaat ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca
     3001 cttgtgaacg taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc
     3061 agccattcgg attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg
     3121 tctggtgttt cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat
     3181 ttttatcgaa taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca
     3241 actcttcttt ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa
     3301 cttcattcat cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac
     3361 gatccacaaa atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt
     3421 ctcgtttacg ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca
     3481 gatcattcgt atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt
     3541 atcataccat aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg
     3601 ccttgaagca aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga
     3661 acattcattt ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt
     3721 tcattataat cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac
     3781 gcttcacaag cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca
     3841 ttcgttgacc agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca
     3901 gtcaaaatca tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta
     3961 aaccgctcgt cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac
     4021 gcgccgaaat aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa
     4081 ggctctaagg taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag
     4141 gcttccttga ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg
     4201 ttatatcctc ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca
     4261 tgaagcattt ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct
     4321 actgcatcaa gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta
     4381 ctttagcata tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa
     4441 gaggtgaaca agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa
     4501 aactctccag catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg
     4561 atcaaacaag attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta
     4621 atcaacgatc aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc ttcttgtgtt
     4681 caagaaaaaa gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa
     4741 aaagaatggc aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat
     4801 acagacacag atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa
     4861 atcaacggta tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt
     4921 ggttctttcc attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt
     4981 tttttaacag agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg
     5041 gctttacatt actatacaaa agatactgta aaagatagta agttgatcaa aagttacata
     5101 gaactgatca aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc
     5161 aatgtcagct ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc
     5221 gaaaaatttc aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc
     5281 tatcttttat tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt
     5341 ttgtcacaac cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt
     5401 ttttcaacaa aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca
     5461 ccataaacag agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa
     5521 tccaagtcac tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat
     5581 ttgtcataga cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt
     5641 acttttcccg atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg
     5701 attgttactt ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata
     5761 gcatagacaa aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa
     5821 aagttcttta ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact
     5881 agcaaaaccc atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc
     5941 cattcctttt gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct
     6001 aatgcatagc taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt
     6061 acgactttta aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg
     6121 aacactaaaa gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt
     6181 ttttcctgct cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc
     6241 atctgatttt gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta
     6301 tgaggccata tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct
     6361 gaactaacgg attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc
     6421 cagctaatga tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt
     6481 cctttatttt ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag
     6541 tatagcggag attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct
     6601 tctcctgaac aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca
     6661 agttctgcgg gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt
     6721 tctgataaaa tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc
     6781 ttcatttgag cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact
     6841 ttttcaccgg gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta
     6901 tattcatacc cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca
     6961 ttgctgtagg gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct
     7021 gccggtttta gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa
     7081 aaaaaatcgg atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt
     7141 aacgttgtcg cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac
     7201 gaaaaataaa ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta
     7261 agatattttc caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt
     7321 caagaaaatc ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca
     7381 attcaagcga cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc
     7441 gcttaaatag cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat
     7501 ggatagaaaa aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt
     7561 cagctaagta gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc
     7621 tgattgattt gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact
     7681 ctcttgcagt aattgacttc attccatatt cttccacgta taccatactt ttgaacagca
     7741 aatattcttt actataattt gttactctcc ccatcattct tacctccgtc
//
LOCUS       DAZKLA010000125         7719 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000125 DAZKLA010000000
VERSION     DAZKLA010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.127"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..2634
                     /gene="adhE"
                     /locus_tag="ACG85N_002403"
                     /gene_synonym="adhC"
     CDS             37..2634
                     /gene="adhE"
                     /locus_tag="ACG85N_002403"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFQ0225120.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2863..3969
                     /locus_tag="ACG85N_002404"
     CDS             2863..3969
                     /locus_tag="ACG85N_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0225121.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            3986..5014
                     /locus_tag="ACG85N_002405"
     CDS             3986..5014
                     /locus_tag="ACG85N_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFQ0225122.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5020..5499
                     /locus_tag="ACG85N_002406"
     CDS             5020..5499
                     /locus_tag="ACG85N_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFQ0225123.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5515..6810
                     /locus_tag="ACG85N_002407"
     CDS             5515..6810
                     /locus_tag="ACG85N_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFQ0225124.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6821..7645
                     /locus_tag="ACG85N_002408"
     CDS             6821..7645
                     /locus_tag="ACG85N_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0225125.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
BASE COUNT         2431 a         1351 c         1754 g         2183 t
ORIGIN      
        1 tttaacaaat aaccatgacc cattaggagg ataatgatgg ctaaagtaat agaaaaagaa
       61 aaaacgaaga caattgacgt acaagcaatg attgatgatt tggcagaaaa agcaaatgca
      121 gcattgaaag aaatggaaag ctttgatcaa gaaaaagtag accatatcgt gcatgaaatg
      181 gcaatggcag ctttggatca acatatgcca ttagcgaaga tggctgtaga ggaaacaggc
      241 cgcggtgttt acgaagataa agcaatcaaa aatatgtatg cgtccgaata tatttggcat
      301 aatatcaaac atgataaaac agttggtgtg atcaatgaag atgtccaaaa agggttgatt
      361 gaaattgctg aaccagtcgg tgtggtttgc ggagtaactc caacaacgaa tccgacttcc
      421 acaacgattt tcaaatcaat gatcgcgttg aaaacaagaa atccaatcgt ttttgctttc
      481 catccaagtg cccaaaaatc ttctgcagaa gcagcacgag tggtacgtga tgctgctatc
      541 gctgccggag ctcctgaaaa ttgtatccaa tggatcgaac atccttcgat cgaagcgact
      601 tctatgctaa tgaaccatcc aggtatcgcg atcgtcttag cgacaggtgg agctggaatg
      661 gtcaagtctg cttactctac aggaaaacca gctttaggtg taggtccagg taacgtacca
      721 gcttatattg aaaaaacagc aaaaattaaa cgtgcagtga acgatttgat cgtttcgaaa
      781 acatttgata atggaatgat ttgtgcttct gaacaagctg tgatcgtgga taaagaaatc
      841 tatgcagcag taaaagcaga attccaagca caccaagttt atatcgtaaa accagatgaa
      901 ttgcaaaaat tagaagatgc agtgatgaac gaaggaaaat atgcggtcaa tccatcaatc
      961 gttggacatt ctgcgatgga aatcgcaaaa ttggcaggaa tcagtgtacc aaaaggaacg
     1021 aaaatgctga ttgctgaatt ggaaggcgta ggcccagatt atccgctatc aagagaaaaa
     1081 ctttctccag ttcttgccat gatcaaagct aacaacacgg accatgcgtt tgatttgtgt
     1141 gaaggtatgt tgaatcttgg aggattagga catacagcag ttatccattc agaaaacgaa
     1201 gagttgcatg tcaaattcgg tcttcgaatg aaagcttgtc gtattttagt gaatacacca
     1261 tctgcagaag gcggcatcgg tgatatctat aacgaaatga ttccgtcatt gactttggga
     1321 tgcggttctt atggtaagaa ctctgtttct cgtaacgtct ctgctgtaaa cttaatcaat
     1381 gtcaaaactg tagcgaaacg gagaaataac atgcaatggt tcaaattacc gccaaaaatc
     1441 ttctttgaaa agaactctct attgtactta gagaaaatgg aaaacgttga acgtgtcatg
     1501 atcgtttgtg accctggtat ggtccaattc ggttactgcg atacagttcg tgaagtattg
     1561 gcacgacgta aaaatgatgt gaagatcgaa gtcttctcag atgttgaacc gaacccatcg
     1621 acaaataccg tatacgcagg aacgaaactg atggcagact tcaagccaga tacagtaatc
     1681 gctcttggag gcggttctgc aatggacgca gctaagggaa tgtggatgtt ctatgaacac
     1741 ccagacacat ctttctttgg tgcaaaacaa aaattcttgg atatccgtaa acggacatat
     1801 aagatcgaca aaccagaaaa aacacaattc gtatgtatcc cgacaacatc agggactggt
     1861 tctgaagtaa cgccatttgc ggtcatcact gacagtgaaa cacatgtgaa atatccgtta
     1921 gcagattatg cattgacgcc agatgtcgca atcgtcgatc ctcaattcgt catgtcagtt
     1981 ccagcatctg tcacagcaga taccgggatg gatgtgttaa cacatgcaat cgaatcctat
     2041 gtttcagtca tggcttcaga ttatacacgt ggattgagcc tacaagcaat caagttagtc
     2101 tttgactatt tagaaaaatc agtaaaaaca cctgatatgg aatcacgtga aaaaatgcac
     2161 aacgcttcta caatggctgg tatggcgttt gctaacgcat tcctaggtat ttgtcactca
     2221 gtcgcacata aaatcggcgg ggaatacggg atccctcatg gacggacaaa tgcgattctt
     2281 ttaccgcata ttatccgcta taatgcaaaa gatccacaaa aacatgcgat gttccctaaa
     2341 tatgactatt tccgtgcaga tacagattac gcagatatcg caaaattctt aggtctgaaa
     2401 ggcgaaacaa cagaggaatt agtcgaagct ttagcaacgg ctgtttatga attaggaaaa
     2461 tctgtcggca tcaacatgag tctaaaagct caaggagtca cacaagaaac actagataca
     2521 accgtagacc gtatggctga attagcttac gaagaccaat gtacgacagc taatccgaaa
     2581 gaaccattga tctctgaatt gaaacagatc atcattgatg cttacaacgg ataagcaaaa
     2641 gaaagcgtag aaaaaagata agaaaataga tgaaaaagaa cttggaaaac gttgtttctc
     2701 caagttcttt ttttaatttc caagtaactg aaagttactt ttgttattta acatttaggt
     2761 caattttaga cagtttttgt tcaaaaagcg atatttatag tggacggtat tactgctata
     2821 ttaaggatat aaaatgaaag cgtttaaaaa ggtggcgtaa agatgattat taaagaagag
     2881 atttttgtac ctaaagaatt agtgaaagaa aaaattgacc tgctcgttga aaacctgaca
     2941 acgatcaaag acgataatgg tgaatttttg ttggactttg atggactgaa agtagatgac
     3001 aaaagctgga cagtgtggaa ttggccacaa ggtgtcggtc tatatggtat ttataaaaat
     3061 taccagatga caaaaaatcc tcgcgcttat caagtggtca atgaatggtt tgaggatcgt
     3121 atggaagaag gcgctccgcc caaaaacgtc aatacaatgg caccgctatt gactatggct
     3181 tacttgtatg aagacacgaa agattcacgc tatttgcctt atttagaaca atgggcagaa
     3241 tgggtcatga atgacatgcc tcggacaaat gaagacgggt tacagcatgc cacatatgga
     3301 ccggaaaata agaatcagtt gtgggacgat acattgatga tgacagtgct tcctttggca
     3361 aaaatcggga aactgttgaa tcgcccagaa tatttagaag aagctcgtta ccagtttatg
     3421 atccatatca aatacttgat ggataaacga acaggattgt ggtatcacgg ctggacgttt
     3481 gaaggaaatc ataactatgc cgaagcattt tgggctcgtg gaaactgctg gttgacgatt
     3541 gctattccag agatcattga aatcttagaa ttatcgaaag aagatgcatt gcgcaaatta
     3601 ttgatcgaaa cacttgaagc acaagtgcgt gcattaaaaa cttatcagtc tgaaagcggt
     3661 ttatggcata cgttgttgga tgatccttct tcttacgtag aatcttctgc aactgcagga
     3721 tttgcttacg gtattttgaa agcggtccat aaacgttatt taccacaaga atataaagaa
     3781 gtcgcttatc gtgctatcca aggattgctt gaacaaatcg atgaaaaagg agaagttcaa
     3841 aacgtttcaa tcgggacagg aatgggggac agtttagatt tttatcggaa tatcggaatc
     3901 acagcaatgc cttatggaca gtcgttaacg gtgctttgtt tgacagaatt gctcgtctct
     3961 tattgttaaa agaaaggaaa cccatatgaa aaaaacatca ctgctgtttg cagcaagttc
     4021 gcttagccta ggtctgctag gtctaagcgg ctgcggaaat aattctagtg tggcaactgt
     4081 cgcagatgca gatgtaaatg agatagttac cttgcgcttt gcttatgcaa gcaacagcca
     4141 gccagtaatt gatgcaatga atgaatttgg gcgtttagta aaagaaaaaa cagatggttc
     4201 cgtacagatc cagtatttcc cagatggaca actgggtgga gaacgtgaac tgatcgaatt
     4261 gacacagtca ggagcagtag atttcacaaa agtaagtgcc tcagcgttgg aaagtttctc
     4321 taaagattat tcgatttttt cagttcctta tctattcaat gatgaagaac atttctttaa
     4381 ggtgatggat aatgaagaaa taatggagcc aatctaccaa tcgaccaaaa atttaggatt
     4441 taccggattg acctattatg attctggtca gcgcagcttt tatatggtag acggtccgat
     4501 ccatacgccg gaagatttga agggaaagaa gatccgtgtg atgcaaagcg aaaccgctat
     4561 caaaatggta gagcttttag gcggttcgcc cgtacctatg ggaagtgatg aagtgtacac
     4621 gtctcttcaa tccaatttga tcaacggatc agaaaacaat gaatttgtct tgtatacagc
     4681 tggacatggt ggtgtggcta aatactattc ctatgatgag catacacgtg tgccagatat
     4741 catcatcatg aatgatgcca tcaaagaaca gctaactgat gagcaggaaa aagccattga
     4801 agaagcagcg aaagaatcaa cggtttttga aattgaagct tttgcaaaag caattgaaga
     4861 agaaaaagct ttagcacaag aagaacatgg agtccaattc aatgatgtag ataatacacc
     4921 gtttcgagag gcagttgccc cacttcatga tatattcaaa aacgatccta cctacaaaga
     4981 cctttatcaa aagatccaag agcaaggagc gtagctgtga tgaaaatgat tttaaacaag
     5041 gtattagaga tcctgggagc tgttttgctt gttgggatgg tcgctgttgt tttatggcaa
     5101 gtattttcca gaacggtttt aggaaatcca aatacggtca cggaagagct tgttcgattc
     5161 ggtcttgtct ggtttgccat gttgtcttcc gcgtatgttg tgggacaaaa agcacattta
     5221 gccgtaacgg ttttaagtga aaaattaact ggcaaaaaaa gtaaaatttt agaagttgtt
     5281 gttcaaagtt tatttctggt atttgctgcg atgatcatgg tgtatggcgg atggaatgct
     5341 gtcacattga caatggggca gatctcgcct tctttaagta ttcctatggg atatgtttat
     5401 ctgtcagtac ctgtatcggg ggtactcatc attatctata gcctgatcaa tctaatcgat
     5461 acattaagca acaagccagc aattcaagaa aaaatataaa aatggaggaa taaaatggca
     5521 atagaagccg gattagtgtt attttttgta tttgttgtgt tgcttgtatt agggatgccg
     5581 attgctataa gtattgcatt ctcatcgatg atgactttat tgttagttat accgtttgat
     5641 gtttctgctt ttagttcagc tcaaaaaatg gtgggaagta tcaatagttt ttcgctgatc
     5701 gctattccgt ttttcgttct ctcgggcatt atcatgaata atggagggat agctaagaaa
     5761 ctagtggatt ttgcgatgtt gttcgtcggg cgcgtgccgg gggctttggc tcatacgaat
     5821 gtgttaggga atgctttatt tggttccatc tctagctcgg ccattgccgc ttctaccgcg
     5881 attggcggag tgttgatccc tcagcaagtg gaagaaggat atgaccggaa gtttgcgaca
     5941 gcggtcaata tcgcgtcagc tccgacaggt atggtcattc ctccaagtac tgcttttatc
     6001 atgtattcac ttgttgctgg tggtgcttcg atctctgctt tgtttatggg aggttatctc
     6061 gtaggcgctt tgtgggcgct aggaatcatg ctagtagcat tcatcgttgc taaaaagaat
     6121 aaataccctg ttgtcgaaaa aggacagatg gctcatgctg gaaaaataat aaaagaggcg
     6181 attcctagcc ttctattgat tgtcatcatt atcggtggta tcttgacggg gatatttaca
     6241 gcagtagaag cttcagcgat agctgtcatt tactccctgt tgattgctat gttttactat
     6301 aaaacggtaa aactaaaaga tatacctaat atgttgtttc aatcagtagc aatgtcaggg
     6361 acgatcatgt tcttattggc tacttcttcc atgatgtctt tcgccatggc gtttaccggt
     6421 attcctcagg caattagttc gttgattatg ggcgtaacgg ataataaatt tattattctg
     6481 ttattagtca atcttgtgtt attactggtg ggtatgttta tggatgtggc gcctgctatc
     6541 ttgattttta caccaatatt tttaccaatc gtcacaagtg taggggttga tcctgtacat
     6601 tacgggttgt tttcaatcat gaatctgtgt gtaggctcca tcacgccgcc agtaggtaca
     6661 ggactatatg tcggtgcaag tgtaggaggc gtaaaagcag aggcaatgct gaaaccgctt
     6721 cttccttttt atggggttat cttagtcgtc ttgtttttga ttacttactt cccgcaaatc
     6781 gtcatgtggc tacccaatat gctgagttag gagaatagtt atgttgaaca ttacaattag
     6841 aggacatgat ctttccgaag tccaaacgat agaagatctt gcagaaaaaa caaaagaaca
     6901 agggattcat acactgcagc tcgccttagg tctttcgttt cctgaaatac cttctggcgc
     6961 aaatgagatc aattctggga tgggaaatta tgtcaaacga gttttagaaa agcaagaagt
     7021 gaccgtagga attttaagtt gctatatcaa tatgattcat ccagatttaa cgatacgaca
     7081 agagttgctg acaaagttcg agcagtatct tcgctatgct tcttcctttg gtgcgtcgat
     7141 ggttgcttcg gaaactggat gtgttttgcc ggaaatccaa tatacggaag aaaatttcac
     7201 agatgaagca tttgctgaag ctgtgtccgt tatccgtcgc ttggtaaaag ctggagaaaa
     7261 ataccaaatg atggtgggga tagaaccagg tttgaatcat ccggtgtatt ctttagcaag
     7321 ggtagagcag ctgatccaag cagtagattc ggactatcta ggaatcattt tagatccgac
     7381 caatttgatc acaagtagtg attatcaaga acaagtccaa ttggtagaag aagcttttga
     7441 acgttttggt gaaaagatat gtgcggttca tttgaaagat tttcgtgtcg aacaggaaaa
     7501 aatcgtgccg gtaaatttgg gagacggtgt gatcgagtac acaaaaatca aagaaatcat
     7561 catgaagaat cgtccgtacc tgtatgtggt tcttgaagaa acaaaagatg atgggattcg
     7621 ttatggaaga agtcttcttg aataatgtaa agaagacgtt ggttaactaa tatttagaat
     7681 ggagggggcg gggctagaag atgccacgtc tcttctttt
//
LOCUS       DAZKLA010000126         7670 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000126 DAZKLA010000000
VERSION     DAZKLA010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.128"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..813
                     /locus_tag="ACG85N_002409"
     CDS             <1..813
                     /locus_tag="ACG85N_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFQ0225126.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            786..1229
                     /locus_tag="ACG85N_002410"
     CDS             786..1229
                     /locus_tag="ACG85N_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225127.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2363..3331
                     /locus_tag="ACG85N_002411"
     CDS             2363..3331
                     /locus_tag="ACG85N_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0225128.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3341..3829
                     /locus_tag="ACG85N_002412"
     CDS             3341..3829
                     /locus_tag="ACG85N_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0225129.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4125..4406
                     /locus_tag="ACG85N_002413"
     CDS             4125..4406
                     /locus_tag="ACG85N_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225130.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4408..4824
                     /locus_tag="ACG85N_002414"
     CDS             4408..4824
                     /locus_tag="ACG85N_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225131.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
     gene            complement(5579..6727)
                     /gene="tnpB"
                     /locus_tag="ACG85N_002415"
     CDS             complement(5579..6727)
                     /gene="tnpB"
                     /locus_tag="ACG85N_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFQ0225132.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(6744..7148)
                     /gene="tnpA"
                     /locus_tag="ACG85N_002416"
     CDS             complement(6744..7148)
                     /gene="tnpA"
                     /locus_tag="ACG85N_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFQ0225133.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            7329..>7670
                     /locus_tag="ACG85N_002417"
     CDS             7329..>7670
                     /locus_tag="ACG85N_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HFQ0225134.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTV"
BASE COUNT         2711 a         1326 c         1247 g         2386 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcccttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tttctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgcgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaaatagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaact cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctcgaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaagc
     1441 cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca aacgatacgt
     1501 cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat ttggctagca
     1561 atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt cgagtgagcg
     1621 aatccaaatt tgcaactctg aagctttgca aatgagtgtg ccggcttccg taaaagtcta
     1681 ggtaacatta gtcgatatat acatagggct ctaatttgcg ttttaaggcg ttttaaacgt
     1741 tctggtatgt aattatacca aagcgacttt aaaacgcctt aaaacgaatt ttagaggttc
     1801 tgtagaacgt tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg ctttaaaaac
     1861 aagataaggg attttatcta gacttgtctt aaaacgccgt agatgagcgc tcagagcttc
     1921 taaaaggact ccttacgaac tgtttttaag caaaggagcc tttaggcgac tgcgcagttt
     1981 aatgtgagag cgtagcgatc acacctttaa agcgaccgta gggagcagat tcccagttta
     2041 ggaaatttcg cgaacctgtt tggagaaaaa aagtaggaaa attttaggcg ggaaattttg
     2101 cccccctctt ttataatctc ttttataacc tctttttaaa cctctttacc gtcggctgta
     2161 tccttactct cccaaggtgt gcagaatctt ttaggggtct ttttgtctgt ttttaggggt
     2221 ctttttgtct gtttttaggg gtctttttgt ctgtttttag gggtcttttt gtctgttttt
     2281 aggggtcttt aattaggggg ttgaccccca ttagaaagct ctttataatt ttgttaagta
     2341 gttaatcttg aaggaggatt ttatggcaaa tgaaattgtg aaataccacc atgaattaaa
     2401 tacgattcct ttacgcaaat ttacgtcagt agaaatgaat ttattcttct ctataatatc
     2461 tagaatgcgt gatgtaggcg ataaaaaagt acaatttaca ttcgaacaat taaaagattt
     2521 aagcaattat aaagcaactg caaatgttcg attcatagat gatttagaaa caacgtatga
     2581 caaattaatg gatttacgtt ttggtagaag aagtgctgac ggtttgcaac gtgaacgatt
     2641 tgttttattt aatcaattta aaatcgatgg aaaagctgat attccttttg ctgaaataca
     2701 agtccatgag aaagccttac ccttacttaa taatcttgaa gaatgggttc gatattctct
     2761 ccaacaattc aatgaattag aaagcagtta ttccaaaacc atgtttagat tattaaaaca
     2821 atacagaacc aaaggaattg ccaatttttc aaaagaagac tttcatgaat tacttgatat
     2881 ccctaaaagt tatagagaaa gtgatataaa taaaaaaatt ataaagccta ttcgccaaga
     2941 actaaccgca atcttcaaag gtttgacaat ccaaaagaga tacggaaaag gacggggaaa
     3001 gcctgtcatt ggctatcaat ttacatttaa acctgagata aaaaaatcag acgactttaa
     3061 caaacatgct ccaaccaaag aagaacaaaa taaagctttg gaactagcta atcaacaggt
     3121 tcaatctgaa aaagaaccta cttcattaca agaaaaaatc aaagaacaaa aacgaaaatt
     3181 agaactttta caacagttag cagaattaga aagaaaacaa aataccccaa gtaagaaaat
     3241 taacaaacaa atagcagata acaattccga tattacccca gaatttaaag aaaacttact
     3301 agataatctc caaaacttat ttaaagaata aggggttttt atgacagata aaactatcaa
     3361 agaattagca gatattctag gtgtttcaaa agataaagtg aagtatcaac tgaggaaatt
     3421 acctagtaat tttacctaca aaaaaggaaa tattacctac ctgaaaaaag aagcagtaac
     3481 aacaattaca aaggttttat caggtaatga atcgggtaac ttacctagtg aattacccga
     3541 ttcattacct accgattacc tactcaagga gattgaaact aagaataatc atattgaaga
     3601 aaaagatagg caaattgaaa aacttcaaat agctttagaa acgcagcaaa aattactcga
     3661 ccagcaacaa caactcaccc tacaagccaa tcaacaaatc aaaaaacttc acgaacaact
     3721 ccagctaacc tatgcagaag aatcttcaga aaaagaaccc ctttctgtta ctgaagaaac
     3781 tcaaaaagga caaaacaagg agaaaaaatg gtggcaattc tggcaataac ccaacaattt
     3841 ttattcataa aaattgttgg gttactttct ttttttgctt ttacaagcat agcgacatga
     3901 tctcacgtcg ctatttttta aagagcataa ggaaacgcgg tatcactttc ttacgaaagg
     3961 tgctaggtag gctgtgccta ccacgtttct aagaaaaaaa tttgctatca ctttttcgtc
     4021 attgtggata gcttatgtat agtgtttcgg caaaaaacag ctatacaaat actatatttg
     4081 tatgatatac ataaattata cacaaatgcg aaaggaattc ttctatggac attttagttc
     4141 ggaatattga tccaaaagtt atttcagaaa tagatagacg ttgccaagaa ctcacaacaa
     4201 aaactggcaa aaaatggagc cgtaatgcgt acctaaaagt tctaatcgaa aacgattttg
     4261 accaggcact taggcattac aaaaaagatc aattcgattt attactcgaa aaatttatcg
     4321 atatacaagc aaaaaatact gaaacattga acgactatgt tcatacaaac aaccaactca
     4381 ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa acacgttacc
     4441 ctttatccca gtcacattga cgttttagat tccatcatcg aaaaaaatcc agatatcaat
     4501 aactactcgg aagccattcg ttttctatgt atgagttatg aacctaatac taatacgaca
     4561 actaccagca accaagcaaa actaaatgcg atgggaaaag aaatttctat tatgtccgaa
     4621 atcattgcag aatttgctga tttatatcta aaagatactg gtgttcttat cggtacaaat
     4681 tctaaagtat atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc ctcccgacga
     4741 aatcaaccca aaaatcgtca aaaagaaacc aatcaaacat tcacttctag aaattttgac
     4801 ccaacaaact ttctaaaaag atgaaaaaga aaccgaaaat tttttctatt tctcagtttc
     4861 ttttttaggg ggcagtcttc cagcaagatt tgcaagttac cggtaacttg cacaaaaaaa
     4921 gaaaaatccg cttgtgcgaa tccttctttt tgcattttgg ggcgctgccc caatctccaa
     4981 atatcggtta tttaatagcg tggtaactca aaaaaataga acgtactctg aaataaaaaa
     5041 agactgctct aacgagcagc caataaaaaa taatgagcgt ttattcttga aaaagcagcg
     5101 cgcttatgaa gcgctgccaa aaatttacca tattttatga aaaataacaa gtttctaaaa
     5161 aggaatcggt taagattcac aaagaatcgt aaacagacaa tagcggaaaa tcaagcttcc
     5221 gaacgtacct tttagctaga ccaatgaaaa atcacaaagt gagcccctgt atcggttgat
     5281 acaagggcta cttggcatac tcgacattat gtctactagc tcgataaata acgattatgt
     5341 tcccaaaaca atattttatg aactataaca aaaatttgtt atcgcatatt gtatatttta
     5401 attataggtg tatatcacgt gaactactca ccacttagct aaacctgacg gttcttaacg
     5461 cttgaagtgg gagcttcttg ggaatagagc atacttgata gcgtatttct ttactaacag
     5521 cggtgtctct tatttaccaa gctatccccg tagttcctac ggttcttgat gattggatct
     5581 aagccaatcc catagaatgt agtcttagac cttcggtcag aatattgata ctagcattga
     5641 tatcacgatc atgatgagta tgacaaacag gacaagtcca ttctcgaatt tcaagagatt
     5701 tcttgccatc tttatgtccg cactctgaac aaatctgact agacggaaac catttatcta
     5761 ctttgatgat ttttcgtcca taccagtcag ccttgtattg taatttagtc acaaactttg
     5821 accaagaaac atcagaaata cttttagcta atttatgatt tcgcaacata ccttttgtgt
     5881 ttaagtcttc aatacagata atatcgtgat ttttgatcat ttctgtactc aacttattca
     5941 gaaaatcagt acgttgattc attacttttt catgcaatct agctactttg cgtttttgtt
     6001 tttgataatt tttagcttca aatagattgg tacctttatt tttagccaat aaagcacgcc
     6061 tagacaattt gcgttgttca cgctttagtt tcttttccat tttggacgtg aatttattat
     6121 tatcaatttt ttgtccatca gaaagaatcg caaagtccat aatacctaag tcaataccaa
     6181 ttgtggaatt agttttaggt aattcgataa tttcttcttt acacaacaaa gaaatatagt
     6241 atttaccgct agaatgacgt gatattgtag cagatttgat aatccctttt ggttgtctat
     6301 gaagcttaat tcttactaat gacttcaatt taggaacttt gatgaattta ctatcaatca
     6361 aagcaactgt accattttga ttattcgttg tataactctg aacaggattt ttcttacttt
     6421 tgaaacgtgg aaatccaacg gatttatccc gaaaaaaatt cttgtatgct ttatctaaat
     6481 taagttgggc attggctaaa gcaaggctat caatttcttt caaaaacgga aattctttct
     6541 tgtatttagc tggtgtcgga aaagtcattt ttttagaaga atcattttta acttcttcat
     6601 acgccttttt tcggtcatca agcattagat tgtagacctt acggacacaa ccaaaagatt
     6661 tggcaaagaa gatttcttgt tcctctgttg gatagattct gaatttgtat gcttttagtt
     6721 gttccataaa gttcacctac tttctattta tgattgtttc cttgattttg gatatacttc
     6781 ttaatcacat caattggtgc gccaccagtt gtcagaagac aaaaactttt agaccagaac
     6841 atttctttcc aaagaaattg tttgactctt ggaaagtcac gttttatcaa tcttgagctg
     6901 gcacttttat aggcattgat gaattttgtc aattctgttt taggttgagc tttgaacata
     6961 atatgaacgt ggtctttatc atgattccac tccactaaag taatgtgata cgattctgaa
     7021 attctttcaa aagtagtttt ggcataatca gatatttcat catcaatcac ttgtctacga
     7081 tatttcgtta ctaaaacaag gtggtaataa agaagaaata ctgaatgatt atttgtatct
     7141 aattccatgt tatatcaact catttcttgt atagactgat tgtaacatgg aacaaaataa
     7201 aaaggcaata cgccttagtt gtcggtcttc gagttaccaa cggtaaccct cataccgaac
     7261 ggcattcatc acccacctat agaggatggg cgacttctgc ctaattctgt taaatttaac
     7321 acaaaggcgt gattattttg aaacccaaaa agaaaattta tctaggtctc ctcttttcct
     7381 tgatgattgg aatttttagt ataggaatta caaataccta tgcttcagaa aattctaatt
     7441 tcaatatcaa ctcccaacca attgaagaac aagcaattaa ttttgcgaaa actgattttg
     7501 aacactccat aacttcaatt cttttaatgg aaaataacac cgaaactgat attaactata
     7561 ctcttggttc gccatttaaa atgctcgatg gagaaaatac ggcaaatagt tctattttct
     7621 ttccagtatt ttcagacgct gataatacga ttaaatatgt ttataccgtc
//
LOCUS       DAZKLA010000127         7610 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000127 DAZKLA010000000
VERSION     DAZKLA010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.129"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..430
                     /locus_tag="ACG85N_002418"
                     /pseudo
     CDS             1..430
                     /locus_tag="ACG85N_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249876.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            595..810
                     /locus_tag="ACG85N_002419"
                     /pseudo
     CDS             595..810
                     /locus_tag="ACG85N_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            948..1919
                     /locus_tag="ACG85N_002420"
     CDS             948..1919
                     /locus_tag="ACG85N_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0225135.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            2059..3372
                     /locus_tag="ACG85N_002421"
     CDS             2059..3372
                     /locus_tag="ACG85N_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFQ0225136.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            3415..4281
                     /locus_tag="ACG85N_002422"
     CDS             3415..4281
                     /locus_tag="ACG85N_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0225137.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            4278..5126
                     /locus_tag="ACG85N_002423"
     CDS             4278..5126
                     /locus_tag="ACG85N_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0225138.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            5140..6612
                     /locus_tag="ACG85N_002424"
     CDS             5140..6612
                     /locus_tag="ACG85N_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFQ0225139.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            6629..7501
                     /gene="scrK"
                     /locus_tag="ACG85N_002425"
     CDS             6629..7501
                     /gene="scrK"
                     /locus_tag="ACG85N_002425"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFQ0225140.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         2360 a         1311 c         1657 g         2282 t
ORIGIN      
        1 taagtctact tataatttac tttggaggtt ttgaatgaat gaaattaagc gcaataaaaa
       61 ttcttttggt gtttatttag gagagcctag ctcggaacag ttaaataaag gtgaagctcg
      121 tcatgctcta tgtcgaaata tattttatgg tagaaaaggc aaactttatc aaacttatta
      181 cgacggtatg gaggaacagc tcaacgcatt aggattagta actaatgcta tcatatattg
      241 gaattcacta tatttagaaa aagtctttga acaaattaaa acggaagggt tcgactgttc
      301 cgaagaagtg ttacgcaagc aatttccatt aattagtgat cacattaatt ttgttggcaa
      361 atatagtttc cgctatgaaa aagaattatc gaatggaata ctacgtccgt taaatataga
      421 gaattattga gaatgttcgt cttttggttc ctaccgtgca ttttcgcacg gatgttccag
      481 atactatttt tgaatgtata aaattgaaaa cacaagaata ctaataaatc aacgattctt
      541 gtgtttttaa tttttcacta ccgtgcattt tcgtttctat gttccatgta ccccaatctt
      601 gaaaaattaa ttaaagactt agataaagcc attgacgact tagtcgtggt gctaccggag
      661 tatcaatgtt taaccagtat cccaggtatt ggtcctgttt aggctgctgg aatactcgct
      721 gaaattggtc caattgaacg tttttctgat gaaataaaat tgacaaagta tgccggccta
      781 tattggcgac agagtcaatc cggtaaatct gaatacgaaa atttttttac gaaaagcttc
      841 acgtctaata tctattttaa aataaaaata ttcctgctaa tttggaatcg tttccttctt
      901 ttggtataat aatacctgaa attcttttga gaggagttgt tcgtgaagtg aaaactacta
      961 tgaaggatgt tgctgcgtta gcaggagttg gtgtgggtac ggtatctaga gtaataaatg
     1021 gagtaaaggt gaaagaggtt accaaagaaa aagtaatggc tgcaattagt gagcttaatt
     1081 atgagcctga tgaatatgcc agaggtatga aaacgaatag tagcaataca atagctctta
     1141 ttttacctac gatttggcat cccttctttt ccgagtttgc ctactatgta gaggaagctc
     1201 tcagccaaaa aaattataag ctttttttgt gtaatgcgaa cggcagtgcg gaaaaagaaa
     1261 aagaatatat ccaaatggtc aagcagagta aagtagacgg aattataggt attacctatt
     1321 cagacattga taaatatgta tcatcaaatt taccatttgt cagtatagac cgccattttt
     1381 cggaacaggt ttcttatgta acggcagata attatttagg tgggcaaatt gccgctcaag
     1441 aactacttac tcgtaactgt aaaaacttgt gctatgttgg gggtgtttca ccttacccta
     1501 acgaaacaaa caatcgtaaa gctggttttt acaatatttg tgaggaaaat aaaatagctg
     1561 tgaaggtatt ggatatgcct gaaccaatta gcaatttaga ggaacaattg ctcgcttttt
     1621 tttcggataa tcctgaaatt gatagcattt ttaccatcaa tgatttcatg gctttggatg
     1681 tgataaaagc ccaaaaaaaa ctagggaagg aagcacctaa ggattttcaa attattggtt
     1741 ttgatggagt caagctatcg ttgcaaaaag actatttact atcaacgatt gtacagccga
     1801 tagaggaaat ggccaaggtt tcagtagaaa cactcatggc gatgatttta aatgaagaac
     1861 cgcctaagcg tgtggtagta ccggttttct ttcacgctgg agagacgact aaagagtaaa
     1921 aaaccggttt ataacgattt ttttgagata ggttgacaga aagtgatttc aatgatatct
     1981 taaaaccaat taattggaaa cgttaccaat taattgaaaa ataaaacgga aacgtttcaa
     2041 aaaataggag gaacatcgat gaaaaagaga atgttagcaa ttgcagcaat tttagcatta
     2101 acagctggag gacttgcagg atgtggcagt aaggagaaaa gttcaggtag cagtgatcca
     2161 ggagaaatta agatttgggt tcagttttct gatgaaacgc ctgaaggaaa agcttgggca
     2221 gagattgtga acaattttaa tgatgaatac gagggtgaat ataaagctgt tactgagtat
     2281 attcctcgta gcggaagcgg cggtggatat gaggataaag taaatgctgc aattacaacc
     2341 aatacccttc ctgatgttat tacgttagat ggaccgaata ctgcagctta tgctcattca
     2401 ggagtcattg ctccattaga tgattatttg aaagatgcgg acatggatga tgtattagac
     2461 agcattattc agcaaggaac ctacgatggc aaaatgtatg cgtttggttt ctcagagtcc
     2521 agtgtgggtg tctattacaa taagtcgctg ttcaaagatg ctggaattac agaagatcaa
     2581 ttgccgacgt tagaagatcc ttggacttgg acggaatttc aagcaatttg ccaaaagctg
     2641 actgataaat acaaaacgcc tgcaattgat atgcaaatgg cgtcgggaga cgaaatgctg
     2701 acctacgcat atacaccatt tatatggtcg aatggcggcg atgtcgttaa tgctgatgga
     2761 accaaagccg aagactattt taacggtaaa aaatccgttg aagcgttgga atttattcaa
     2821 gaattggttg aaaaaggtta caccacaatc agtcctattg aaaaaggatt tgaaactggc
     2881 aaatatgcca tgatgctgag cggttcttgg acaatggcag atttagagga aaactatcaa
     2941 gagattgatt atggtatttt gccttacccc gtttcagatg caactgggga gttagtttca
     3001 ccaaccggaa gctggcagtt agcaatgact gagaaaacag agaaaaagga tgcttctgcg
     3061 gcctttatta agtttgcaac cagtacagaa tctagtcgta ttatgagttt agggaacagt
     3121 gttctaccta tcagaaattc aacagttgat ttaattaaag atgaagtgtc ggaaggaatg
     3181 caggtattaa ttaagcaaaa ccaactttct ggacatgccc gcccagtggt tgtagcttat
     3241 ccgcaagtat ctagagcatt ccaacaagca gttcaagata ttagctactt cgaggaaaac
     3301 ccagatgtac aaaaagtagc agatagtcgc gctgctgaaa tgcaacaggc aattgatact
     3361 tcactaaaat agaagtttct aaagggggcc aatcaagtgt taaaaaaacc gacaatgaaa
     3421 gacaatttga taggttacgg ctttctagcg ccagctttgc tgctgctgac gatttttctg
     3481 atcatacccg taatcatggt aatttattat gcctttactg attattattt gttaacgcca
     3541 gatgctagac aatttatcgg cttagaaaat tttaagcgac tgttcgctga tccaattttt
     3601 gtaaaaagta tttggaacac ggctaaattt gttgtttgga ttattccggt ccaaataggg
     3661 gcagctttag gaatggcact tattatcaac aaaaatcgta aaggaaatat cttttttaaa
     3721 gtagcctttt ttgcgccagt cgtgatgtcc ttagtcgtta tttcgatttt atggttgtac
     3781 ttgttaaatc ctaatgaagg tctggttaat acgctatttg caaagattgg aattgcttcg
     3841 cagccttttt tgactagtcc caaacaagca atgtatacgc ttgttttcgt atcggcatgg
     3901 caaggtgcag gatatcagat gctgctgttt ttagggggaa tgcaaaatat tccgcaagac
     3961 gtttatgagg cagccgaaat tgatggtttt tctaagtttc agcaatttcg ctatatcacc
     4021 atgccattat taaagcccac ggcgattttt attttactta ccacattgat ttcggccttt
     4081 aaactaatca ttcagccaat ggtaatgact caaggtggac caatgaattc tactatgaca
     4141 atggtttatt acatttacca aactggattt acagaccgta tggtaggcta ctccagctcc
     4201 attgcgttaa tctttaccac tatgattggt ttaattacaa ttgctcaaag aaaattggta
     4261 aaggaggatg atccagcatg accaagaaat ttggaccgct aatgatcgta gaatattgtt
     4321 tgctgttcct tttagcggca ttattcattt tcccgatgct gtggatggta gtttcctcta
     4381 tgaagcctga agcagatgtc tatcaaaatt tagcttcctt caaagctttt ttacctagtt
     4441 tgaatccagc taattggttt aaaacttatc aagaggttat ttcccggttc agtgtgggaa
     4501 cttatttatt aaacagtatt ttttatggtt taacctttgc ctgtgggtca atcattgtaa
     4561 actctttggc gggttttgcc tttgcgaagg ttaatttttc aggcaagaaa attatctttg
     4621 gagctttgtt agctctgctg attgtcccga tggagacggt tttaattccg caatttacca
     4681 tcgttaattc actaggctta gtgaatactc gcttggcagt gattttacca gcgatggcca
     4741 gcgcgtttaa tatctatctg tttagaaatt tctttatcgc gattccagaa gaaatcattg
     4801 aatcagcgaa attagatggt gcaaatattg ttaaaatttt ctttcacatc atgctgccaa
     4861 tgtctaaacc agccatagcc acagtaggtg tcttatcctt tatctccagc tggaatgatt
     4921 atatttggcc gttaatggtc ttaactgaca gcagcaaatt ttcaatgcaa gtggcaatta
     4981 ctacgatcaa tactacacag ccagtttata tcaatcaagt gatggctgtt ttaaccattg
     5041 caacgattcc actgattatt gtttatatcg ttgcacaaaa atatattctg cagggacttg
     5101 gcggttctgg aacgggtata aaataggagt gaatagtgaa tgttgcaaaa acttttaaag
     5161 gccaatgagt ttattgagaa aaatgctcaa acagtcaatc aagaataccg gaataaattt
     5221 catttgatgg ctccgattgg ctggatgaat gatccaaatg gttttgttta ttatcaagag
     5281 gagtatcact tgttttatca atattatcct tacgacagca tctggggacc aatgcattgg
     5341 ggccatgcta aaagcaagga tctaattcac tgggagcatt tgccagtagc actggcacca
     5401 ggtgaaagct atgacaagga aggctgtttt tccggcagtg ccatagaaaa ggatggcaag
     5461 ctgtatttga tttatacagg acacaatgtt gtggaagggc aagttcgaca agttcagtgt
     5521 ttggcagttt cagaagatgg cattcacttt gaaaaatatg ccggaaatcc aatcatcgca
     5581 gaagatcact tagcttctgt tgcaacgacc gaagatttcc gcgatcccaa agtgtttcag
     5641 cgaaaagaag actattttac tgtagtcgct actaaaactg cagatgctcg tggacaaatt
     5701 gtgatgttcc attccaaaga tttactaact tgggagtttt attccgtatt gctggaggga
     5761 actccagaac aaggcattgt atgggaatgc ccagacttgt ttgaactaga tggcaaagaa
     5821 gtcttgattc tttctcctat tcaaatgact ccacaaggca atgcttacca aaatacgaac
     5881 tcaactgttg ctttcatcgg tactgtcgat tggcaaacag gcaaatttct agtagagaat
     5941 tttcatgaaa tcgatggcgg tttagatttt tatgcacccc agacctgtga aaacgaagca
     6001 ggccaaagaa taatgattgc ttggatgcaa atgtggcagc gtaaaatacc tacgaatgat
     6061 ctgggccatg gttggtcagg ttccatgacc ttgcctagag agctgagggt ggctgataat
     6121 caattgttgc aacggccagt ttcatcaatt tatcaagtaa ttgatccgca ggaaaggatt
     6181 gaatgggcga agactgaaga gaatcgcttc ttactgaagg acttagtata tgaccagcac
     6241 tatttacgct tggaaatcaa tttaaaacag gcaacgaaac tagaacttca ctatgcgaag
     6301 ggacccgacc atacattact gcttaactat gatgttgaac gggagtgctt tactgcttcc
     6361 agggaacaca tgccgtatga tttatcagga gctgaaaagg aagtgttgaa aaagcgcagt
     6421 gtgacgattc ctttggaaaa taattgtcta accatcgaaa ttttccgtga tacttcagct
     6481 attgagcttt ttctaaatga tcgtctgact atgagtacaa ctttttatga gacagaaaaa
     6541 ggacaggatt ttcttttgct ggcagacagt gaagtaacct tttcattgga aagcggctta
     6601 attaatctat agaagggaga gctcagtgat gatttatggt tcaatcgaag caggcggcac
     6661 aaaatttgtg tgtgccatta gcgatgaaga aatgacgatc aaggaaagag tgagctttcc
     6721 gacaacgaca cctgaggaaa caataccttt agtcattgac ttcttcaaac aataccaagc
     6781 agatttagca gggattggga ttggctcatt tgggcctatc gatatccatc gcgattccgc
     6841 cacttatggg tacattacct cgaccccgaa agtggcatgg caaaattttg atttcatcgg
     6901 taccatgaaa aaagaatttc cgatcccgat ttcttggaca acagatgtca atgcggctgc
     6961 ctatggtgag tatgtcttcg gcagtggcaa aggattgagc agtgtcgttt actatacgat
     7021 cggtacgggt gttggcggcg gtgccttaca agaagggcgc tttatcgaag ggtttagtca
     7081 tccagaaatg gggcatatgc tcgtcgtccc tcatccaaaa gatgactttg ctggcagctg
     7141 tccatttcat ggcaactgtt tagaagggat ggccgctgga cccgccatcg aaaaacgctt
     7201 agggaaaaaa ggacaagagg ttgcagaaga tgatccttat tgggaaatcg aagcctccta
     7261 tattgctcaa tgtgcctaca atacaacctt gatgttttct ccagatgtga tcatttttgg
     7321 cggtggtgtg atgaaacaac gccatatgct gcaaaacgtc cacgacgcat ttgcaaaatt
     7381 ggtcaatggg tatgtcgaaa cacctgagtt aagcaaatat atcgtgaccc cagcattaga
     7441 agacaacgct ggaacgctcg gttgtttggc attggctcga gaagcggtct tacattcata
     7501 aacgaaaaaa ggctcttcga catttagtgt tggtagagaa agatacaact gaaaatttag
     7561 tcataaccag ttaggcagag tgcttaactg gtttttgttt tacttggaag
//
LOCUS       DAZKLA010000128         7537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000128 DAZKLA010000000
VERSION     DAZKLA010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.130"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..1248
                     /locus_tag="ACG85N_002426"
     CDS             join(107..335,335..1248)
                     /locus_tag="ACG85N_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0225141.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="ACG85N_002427"
     CDS             1483..1935
                     /locus_tag="ACG85N_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225142.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="ACG85N_002428"
     CDS             1949..2638
                     /locus_tag="ACG85N_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225143.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="ACG85N_002429"
     CDS             2763..5114
                     /locus_tag="ACG85N_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFQ0225144.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="ACG85N_002430"
     CDS             5194..5589
                     /locus_tag="ACG85N_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225145.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="ACG85N_002431"
     CDS             5914..6531
                     /locus_tag="ACG85N_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0225146.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="ACG85N_002432"
     CDS             6572..6871
                     /locus_tag="ACG85N_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFQ0225147.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="ACG85N_002433"
     CDS             6905..7072
                     /locus_tag="ACG85N_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0225148.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..>7537)
                     /locus_tag="ACG85N_002434"
     CDS             complement(7117..>7537)
                     /locus_tag="ACG85N_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0225149.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2594 a         1138 c         1502 g         2303 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgat
//
LOCUS       DAZKLA010000129         7273 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000129 DAZKLA010000000
VERSION     DAZKLA010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.131"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..251)
                     /locus_tag="ACG85N_002435"
     CDS             complement(54..251)
                     /locus_tag="ACG85N_002435"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0225150.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(397..1275)
                     /locus_tag="ACG85N_002436"
     CDS             complement(397..1275)
                     /locus_tag="ACG85N_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0225151.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1314..2432)
                     /locus_tag="ACG85N_002437"
     CDS             complement(1314..2432)
                     /locus_tag="ACG85N_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFQ0225152.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2429..4528)
                     /locus_tag="ACG85N_002438"
     CDS             complement(2429..4528)
                     /locus_tag="ACG85N_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFQ0225153.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4579..5400)
                     /locus_tag="ACG85N_002439"
     CDS             complement(4579..5400)
                     /locus_tag="ACG85N_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0225154.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5397..6206)
                     /locus_tag="ACG85N_002440"
     CDS             complement(5397..6206)
                     /locus_tag="ACG85N_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0225155.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6227..6721)
                     /locus_tag="ACG85N_002441"
     CDS             complement(6227..6721)
                     /locus_tag="ACG85N_002441"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0225156.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6733..7233)
                     /locus_tag="ACG85N_002442"
     CDS             complement(6733..7233)
                     /locus_tag="ACG85N_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0225157.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
BASE COUNT         2212 a         1683 c         1214 g         2164 t
ORIGIN      
        1 cacaactctc tataatagaa ttacgattta ttttatacgg ctgaaaaaat ctatcaccaa
       61 aaaaattcaa acattaagcc aaggattatg aagtcaacat ttcgaaaaat gaacatacat
      121 tggtaagcaa ctataactat taccacagac ataaagtaac acaatatatt actatttaag
      181 tgtacgccac cagtgaatat cctcaaggta tacagcgcac aacaaaaaga aaaaaggaaa
      241 cgcagaagca tttcctttca gtgatttaat gaaaacaaca cgatttgact tagagccttt
      301 ttatttgaat tcattttcgc tttatccaac ttcatttgat catttgatta ctcggttaaa
      361 tacttttgac atgagtatca atgaaacatt cattaattat ggttgcttat ttcttaccgc
      421 ttttctggcc aatgctaatc cgccgattat tccggcatta tcacctaatt cgggcgtaat
      481 aatgtagcta tcaattggtg gtatttcaac atatccattt ataagctcaa caaatttatc
      541 ctgaactttt ttcttcatgt gttcttgttt cattacacca ccgccaaaaa taattctatc
      601 tggcgaaaac atcagtgttg tattgtatgc acattgggca aggtaaaagg cttcaatctg
      661 ccaaaacgaa tcgtcagcca ataaattctg gccttttaca cccaaccgtt tttcaatcgc
      721 tggtcctgct gccatcccct ctaaacaatc ttggtggaag ggacagtttc ctgaaaaatt
      781 gtcctttgga tgtcgcttga tcaacatatg ccccatttcc ggatgactga acccttccac
      841 gaaagtccca ttgaacaagg caccacctcc gatacctgtt cccacagtga agtatgcaac
      901 acctgctaac cctttgccag aaccagcaac atattcacca tagcaagccg cattgacatc
      961 tgttgtccat tcaatcggaa tattgaaata ttgctttagc gttcctacaa aattgaaatt
     1021 ttgccaagca agttttggtg ttgatgtgat atgaccgaaa ctcgttgatt tgcattgaat
     1081 atcaattgga ccaaagcagc cgaccccgat gcctgccaat tgttccttaa aaggtttaaa
     1141 aaaatcaatg acctgcttca ttgtttcaac tggagttgtt gtgggaaaac tgactctttc
     1201 caaaatcgtt aattcctcag taccgactgc acagacaaat ttagtccctc cagcttcaat
     1261 tgccccatat atcatcatag gttcctccct tcgaatatgt gaaaaacact tcattaacgc
     1321 ggcaagcgtt ctcgatcagt gaaatgacgt ttagtatttc ctgaagcttc tgttgaatac
     1381 cagtaagcga cactagcaac atcgtcttgc cgctcaaata agcgaacatc atcgttgccg
     1441 atttgttgta aagtgaccga taaatcttcg gtaaaacaga tggggtcttg aagatgccaa
     1501 cgataaagac catgtcgtgg cattgcgtcg tcaccaaagg catgaacggc attcaatttt
     1561 cccgtttcaa aacgatctct tgtggcatct ctatttgatt ggtaaggata ccctaaaaag
     1621 gctgttgaat agtttttcgc atgtggacgt ccgaactcat cgtactcatg aaaggcccac
     1681 gccccgccaa aataatcttc tgaacctgtt gaggttacgg tcggatactc actatcccca
     1741 tcaatgtaga atttaaactc gccttcgccc caccaataac gttctaggca ggtaagcgca
     1801 aggtaagtac ccacgtaata gccttgaccc tttaatcgat caattaactt ataatcctcc
     1861 tgtaatttgg taatgcgttc ccgatgccaa tttgcgtgaa aatataggct attttcttcc
     1921 aattcgtcca ccaaagcgta atttatcgta taaaaaaatg aggacatatc cttcggatgt
     1981 aaattctcga tcgtaatttt agcggatgtt tggaaaggca tttcgaaata ggaattaaag
     2041 ccccctgtag gattaaccac tatcggcaac gagttcacct cgcaacgctc tccaaaccca
     2101 ttacaaaaga aatcccctaa aggactttca acagcagggc tatccgaccc atcccaataa
     2161 attcttaaga ttaaatcacg taaaacaaaa ctgccaaatt ctgttcgatc acgaatcgtc
     2221 atccacatat gacgaattat tcccgttcca gtaatatccg caatcacagt ttcttcacca
     2281 gaagtcagcg gaattgcgcc acgacctttg cggcttggtc ctagctcgct tggcaacatc
     2341 gcagctttac ctttctcgcc tgttggattt tcgggagtga ttgctcgact ctggattttt
     2401 ttaaattggg taatattttt tagtatactc atctttttct cctttgaatg tgaatcgttt
     2461 ttcccttttc tacgtgaaga atttttttcg tatcacttat tttcaatggc ttttctgaca
     2521 aatttgaaat actcaactcg gattgcgtac attcaatcga aatgaaggct tgaccgaact
     2581 tcaaattctt ccaagaaaac gttaaattat caacaggatc aatttgcaat actccttttt
     2641 tgaatttcaa tccgaaatat tgactgatca ttaggctgac aaaaagaccc gatgcccagc
     2701 cgcatttgcc gcagccaaag tctcgcacga cttctccttg ttgagcctct aagcgatacg
     2761 gccaccacca ccacgaaccg tctaaatctg ccaaacaaat tagttcttct aatcgggtta
     2821 cgaaagcttg agggttgtgc agctcgctca ttaaaacagt gataaagcca ggaaaagtgg
     2881 cgccggattc taatccccaa gtgatgcctt tgatttcctc gctataggtt ggattgaaag
     2941 aactagcaga aaaggccatg gtgttctgat ataaatgatc tttattatct aaaaattgat
     3001 aaaaaggcat caacgtagtg tctgattctt ctccatcatg catcaataaa ttaatttttc
     3061 catccacaat cacatcactt aaaaagatta acccttgctc aagaatcggt ttttgatagt
     3121 gcttcactga ataggttgtt ttctcctcat caccgattcc ctccaaaaat tgctcaccaa
     3181 aaggaccatc gatagtcatt tgtttacaaa ttgattcctt tagtttacag gcaatgtttt
     3241 tgtatcgctt gctaagactc agttgttcta gggcttcaaa aatttctgca agtccaatac
     3301 aggccttcca aagacaaaga ttgctgccgg tatgatactt tccaagtgca aaggcgtctg
     3361 ataaccaaac agatggaaaa agcattggtt cccctgaccg acgttgagct aatatgtcct
     3421 caataattct tgaagcattc agtagaacct gcggacaccg ctcaaagaat tttgtatccc
     3481 cagtatgttc aaaataaaga ctagccagta acgcactgga taacgaatta ctcagagaat
     3541 gattgatacc tcctgtaaat tttgaaccag gaaacttaac gccatattga tcaaaccata
     3601 gaaccgtttt ttgacataac tccggttcaa acatggtgaa tggcaatgat gcgtaataca
     3661 tatccttatt ccaaattcga ttagtagcag gatagctgcc ccaattgctg ccgacgacct
     3721 gtccctgtcg attcataccg aaactagaaa agctttgata aagtgcacga cgatagagat
     3781 ggacaactct atcgtttggc attgacagct gcccaaatag ctgagcaaaa tagttcaatg
     3841 tttccttcag ccatttttcg atatcagatt cctgaaaagt cattacttct tcaaaagcat
     3901 ttgggtccac aaaagcaatg gcgaagcttg ccacttcctg ttgtgcgatc gtgtccttat
     3961 ttcctttttg agaaatcaac acattttgct gatccgaata cttctcttga tacgaaggca
     4021 catctttcac tgaaacttga aggctttcgt gtgtttcatt cacaatgtaa acctgttgaa
     4081 caagcatact taaacgctta ttagccagta tgggagcaaa ggatactaca atgaccttaa
     4141 acgtcgaata atgatgaata tatctaggca aacagtcttg aatcacatcg acttcaatac
     4201 gatgatccga ttctgacaaa tggtagacaa tcccgtttac ttcaaccttt aaggataacg
     4261 aattggtttg agagagctgt ttattgaccc aaacacctgg tttttcctca gttagtaaat
     4321 tttctcgata aaaggtcaac gtttgaatcg tcccgcaaac atcaaggttt gctaatgtct
     4381 tttgattact tacatcgaag gtcaatccct ttacatcagg aatattccga tagtagctgt
     4441 attcgtttga agtagtcaca tttccgtcgt ttaactcagc attaaaaaat gggtgtttgg
     4501 gtgaataaat tacttcatta atagtcatct tttccctcct tgctaaaaaa actgcagcct
     4561 agtagctgca gcttcctact atgcgattcc aagtaatgac aataaaattc ccaagacgac
     4621 cattccaaaa ataattttga tgacgccgac ttttttatct agtaatttga aacagctcaa
     4681 tgttaccaat aacggtacaa ttcctttaaa aatactatcg aggatttctt ggacattcat
     4741 tagttcttcg cccttcattg aaaagctcaa aacggtattg aatttgacca tactgatcgt
     4801 cattgcacct accatgatca gacccaagat actagcagct tttgtcagaa tttcaattaa
     4861 cccgttcgca tacagctttt caatatattt agagcctaat gaatagccta aaacgccgcc
     4921 atagtatcgc atcaagtaat taggtatatt gaacataact aaaaagataa ttggtgccaa
     4981 aatatttccg gcattggcta agttgatcgc tagtcccgcg gtaacgacac gccaaactcc
     5041 ccagaacaat gaatcgccaa ttccagccaa tggtcccatc aaactcgttt tgatcgcgtt
     5101 gatactttcc acgtcaaaac cagctttttc agaattttcc ttctccatcg atgccgcaat
     5161 ccccatgaca aaggacgcgc aaggaatggt cgtcgaaaaa tactgtgtat gacgttccag
     5221 cgccgcagct cgatcttctt cacttttgta gaaatgatta atgaagggca tcatagaata
     5281 ggcgaaacca ccgccgccca tcgtcaccgg attcaccgaa gcatacatag tcatcgagcg
     5341 ccaaaacatc ttccgaacca attttttctc atctttagaa attccatcag ctaatttcat
     5401 tgaaagaaat cctcctcttc ttggtcaagc gttgcattta cagcagcatc gctttgaact
     5461 gcatgaacag atatattttt tagttgtaga tctctttggg ctataacaat tgcaataatc
     5521 gttccaactg cggcaattgc aatcaatggt aactcgagat acgccataac aacggcccca
     5581 aagaaaaacc acacagcaat atttttactc caaagcatcc gtaacagcat cgccatccca
     5641 actgccggca gcagatttcc agcgactgtt aatccgttca tgatgacctc tggaatatta
     5701 tccaagacgc ttttgacaac atctgctcca aataaaacgg caaagaatgg aataagagca
     5761 taaagcgcat agtggacaaa ccacataaag aagtgcagat tgatgattcc tttttgatca
     5821 ccagattttg caaatttgaa aaatttagca gccatcggtc cacatacaaa agaaaaaatc
     5881 atattcatta actgtaatcc taatactgct agtggaaaga caatgggaat aatcgcatcc
     5941 acgtttcctc ccattttgat tgcgaacgct gtagccagcc cagcagccaa agaaggctcc
     6001 gccgtactag agattccgat attgacgaca cccataaaga tggcttctag tgacgccccg
     6061 acaatcaacc ccgtctgcaa atcccccatc aacgctcctg ctaacggagc aacaacaatc
     6121 ggacgactca gcatggacat cccgaacatt tcatgtccag caactccaat aaaaacggcc
     6181 aaagcgacta caaatgcatc atacataatt tttctccttc ctttttctaa aaatcactca
     6241 aaatagagat agctgaacgt ttttccatcg ccggtgtagg ctgaaaaacg gtatctggat
     6301 aaagttcaac taattctttt aagtcttgaa tttctgacgt cgttaacatc acttcctttg
     6361 ataaggtttt cttatcttcc gatggcggtg tcattttccc agcattcccc acgttcatta
     6421 attcgatttc cgttgggaaa gctttcgcaa cttttaccgc atctttgaca ttagggacta
     6481 aaaccatcaa gcgcatgtcc ttcgcccgcg gatcttttaa caaatgaatc gcgtcatcaa
     6541 ctgttttaat aatgactttg acattcgatg gtgcagccat cttcaatgtc atcttttgaa
     6601 tatcgttggt agccgcctca tcgcttgcta ctactaaccc ggttaaattt agactctttg
     6661 tccaagtcat cgctacttgt ccatgaatta atcgttcatc aattcgcact gctttaatca
     6721 tttttcttcc tcctaaaaaa aatcttcctc ttcatttact acatgtacct catcaagaat
     6781 ggtcatcttt aattcctgtt ggcatttatt taccagttcc tgaatatcct cctttgttaa
     6841 ttccttggtt gccaatacta tttcgagcag aattggcaga ttaaagccag acaaaatagt
     6901 tacagcaatc tctttctcca aaaaggtttt ggtaatcttc tgattgacac ttccgccaaa
     6961 taaatcggta aaaacaaaaa tctgatcctg ctcgttcacc tggtttacaa atacattcaa
     7021 ctcctgttca aaatcacact catccacata agcattaatc accgtaatct cttgaacctt
     7081 ccccgtcagt aattgaatac tgctaagaaa gcctgaagct aagtgaccgt gagtcgctat
     7141 tagaattttc ttcatatggt taattccttt ctttctgttc acttttatat aagcaattcc
     7201 tgtgccaact tttaaaaaca ttggtttaat cacgttaatt cacattaaaa aaagtgtcta
     7261 acctaaaaaa aac
//
LOCUS       DAZKLA010000130         7230 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000130 DAZKLA010000000
VERSION     DAZKLA010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.132"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1140
                     /locus_tag="ACG85N_002443"
     CDS             <1..1140
                     /locus_tag="ACG85N_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225158.1"
                     /translation="ADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNIL
                     LALVRYVNWEGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRK
                     AFPLGLKEEDKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENM
                     ELLRKDFEVVYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQ
                     VFLPERIYLLSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQA
                     GRQEIEIFVVSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVV
                     LCDAASYQNGLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1361..3124
                     /locus_tag="ACG85N_002444"
     CDS             1361..3124
                     /locus_tag="ACG85N_002444"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFQ0225159.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3208..3666
                     /locus_tag="ACG85N_002445"
     CDS             3208..3666
                     /locus_tag="ACG85N_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFQ0225160.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3775..4458
                     /locus_tag="ACG85N_002446"
     CDS             3775..4458
                     /locus_tag="ACG85N_002446"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0225161.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4605..5219
                     /locus_tag="ACG85N_002447"
     CDS             4605..5219
                     /locus_tag="ACG85N_002447"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFQ0225162.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5235..5441
                     /locus_tag="ACG85N_002448"
     CDS             5235..5441
                     /locus_tag="ACG85N_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225163.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     regulatory      5666..5832
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            6006..7112
                     /locus_tag="ACG85N_002449"
     CDS             6006..7112
                     /locus_tag="ACG85N_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0225164.1"
                     /translation="MIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLSWSVIFLAR
                     SVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQFQPSELANL
                     FLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIAWVIFWAAA
                     VPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDSFIDSHGAG
                     YQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIGALCVLFLL
                     FSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGVPLPFVSYG
                     GTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
BASE COUNT         2473 a         1139 c         1478 g         2140 t
ORIGIN      
        1 gctgatcttg aatttatttc tttgagcaaa gtatatacaa tcaaaaaaat cgtattggat
       61 ttttttaaag aaaatgatta tttgaaaaat aaagaaatca ttatcccaga atttgactcg
      121 cggaacatct tgctagcttt agtacgttat gtcaattggg aaggctatga aaataaaaat
      181 ccgtatgtag aagaagcttg taacgagttg attgattatg tagagactca tttttttaaa
      241 agaagatatt cggaagatga aaagtccttt atcatacgag gaatagagat cgcaatcgga
      301 agaaaggcat ttccccttgg actgaaagaa gaagataaga aaagcgcaga aacgaagccg
      361 ttatttcaat tagtctgtca aggattggaa gagcaaaaag gaattctaga cttacaagaa
      421 gaagatgtct attatatttt ttctttgttc aacagtagaa attataccaa tgagaacatg
      481 gagctgttaa gaaaagattt cgaagttgtt tataaaaatt ttgttctcaa tgacctatca
      541 ctgaatgaat tgattttaca gattattagt atcgctgaaa tcgacgatgc agaaaatttt
      601 attttcaagc aatccttttt acaatttgtc cgtacgttgt gggcagatgg acaagtattt
      661 ttaccggaaa ggatctatct tctatcagaa aaagaacaac ttctgtacgg aaaaattgta
      721 gaaatattgg aagagtggaa aaagaaaaat caaatccaac taagatggaa tgaaaatttc
      781 attcgaaaat tcgtcaaaag tctttctttg atcaacagtg aacaagcggg gagacaggaa
      841 atagaaattt ttgttgtttc agaaagttca gtcaaacaat ttttttatcg aacccaattt
      901 catttgtatg cggaagaagg catcgctgaa ataaatccta ttttatttcg ttcattagaa
      961 gaattgcctg atgaatgtct atgtgcgaaa aaaagagtgg ttttgtgcga tgctgctagc
     1021 tatcaaaatg gattagaaac tgaaaagaca aagatctatc caatctcatt aaaaggtgca
     1081 aacatccatt tgtatcatat tgctcaggaa cttcacctat taaaaaaaca tatttcttga
     1141 tgagacctta attaggagac cttctacaaa gctttttaat tgtctgtaga aggtttttat
     1201 ttgagtcctg attcgtgcga agatctactt atataacaag ctttctctga aattttttta
     1261 aaatgctatt gacttagagt caactctaag tagtagaata gataaagtgt aacaaatcta
     1321 ttttcattaa aagaagatag tacggaggag agaatgtatt atgagtacag tattacatgt
     1381 attggatgca atcaatagcc ccgcagatgt caaaaaatta tctgttgagg aaatgaaaca
     1441 gttggcagaa gagatgcgcc aaaaaatctt gcaaaaggac agcgatgtag gtgggcacgt
     1501 tggtccgaat ctaggtgtag tagaattaac gattgcgttt cattatgtat ttgattcacc
     1561 aaaggataaa atcgtttggg atgtttccca tcagtcttat ggtcataagc ttttaactgg
     1621 tcgaaaaaat ggttttgaaa aaggacatta ccgtgaagtg agcggatatt ccaaccaaca
     1681 tgaaagtaaa catgactatt ttacagtggg gcacacatct acatctatca gcttagcaac
     1741 aggtttagct aaagcgagag atctgaagaa agaatcagga aatgtggtag catttatcgg
     1801 tgacggctcc ctaagtggtg gcttagcttt tgaaggattg aacaatggtg gtgttttaca
     1861 atccaatcta atcgtattag taaacgataa tgagatgtct attgatgaga atcatggcag
     1921 tttatataaa aacttagctg agcttcgtca aaccaacggt aaaagcagca ataacctatt
     1981 taaagcaatg ggatttgatt atcgttacgt ggataatgga aacgatctgg aaacggtgat
     2041 ccgtttattt gaagaagtaa aagatattga tcatccgatt gtacttcacg tccatacgga
     2101 aaaaggtcgc ggttatcagc cggcaatcga taataaaatg aaatatcatt ggcatgtacc
     2161 atttgatctg tccaccgggg aggcaaagca agtaacttct ggagaaacat ttaatatgat
     2221 catcatggat tatttggata agaaaatgca aacaagtaca gctcctattg ccgcagtcaa
     2281 tgcagcgata ccaggcgcct ttggactgaa aacattccaa gaaaaacatc cagatcatta
     2341 ttttgacgtg gggatcgctg aacagcactc tatcactttc gcttctgctt tggcttctca
     2401 gggagtacgt ccagtcgtct tccaatcaag tactttcttg caacgggcat atgatcaatt
     2461 atcacatgat ttagcgatca acgagaatcc agtgattatt ttagtcggtg gcggaacgat
     2521 ctcagctagt gaccgaacac accaaggaac atttgacatt ccttacttat caagtatccc
     2581 gaacatcaaa tatcttgctc cagcaacaaa agaggatatg atctctatgc tagattgggc
     2641 gttcgaacaa acagcgcagc ctgtagcaat ccgaattcct acacatggtg tagaacatgg
     2701 tacagctatc gcaaatgatt atagtattcc agcctatcaa acagtaaata atggaggaaa
     2761 agtagcaatt ttagctttag gcggttttct tgaattagga aaagaaacag taaaagcttt
     2821 tagaaaaaaa ggaatcgaag caacgttgat caatccagga acgatcacag aaaatgacgt
     2881 agaaacatta gataaattaa aagaaacaca cgaattagtc atcacattag aagatggtag
     2941 tttgtcagga ggatttggcg aaaatatcag tcgttattat ggtgattctt caatgaacgt
     3001 attaaatttt ggtgcagaga aagaattcac ggatagcgtt cctgtagggc agctttatga
     3061 acgttatcat ttaacaccaa aacaaatcgt tgaagatagt ctgcgtgtct tgaatattca
     3121 ataattcatt atgctcacca tccagataat tgtttatact agtatctgga tggttttttc
     3181 attataaaaa gcaaaggggt ggatgttatg tatcagcaag tagaagaata tgtcaaaaag
     3241 ttccaagaaa aatatcctag ttatcaatca gaaaaattag gtttcttttc atttggggat
     3301 acagaaaaaa tggcagacga gttgagcgaa ctagttgtaa aaaagataaa gactggtaca
     3361 tcaagtggtt acgatctgta taaacaagag aatgagcctt taccaaaagt tggtcaatta
     3421 gacgtggtcc tgaatggaaa aaatgaacca gtttgtatca tcaaaaatat aaatgtgacc
     3481 atcttgcctt ttagtgaagt cacgaaagag catgcttgga aagaaggaga aggggatcgg
     3541 acattagctt attggcggaa agtccatgct gagtttttta aacatgctta taaagaagcg
     3601 ttgaacatag aatttacaga agagaagctg gtcgtatacg aagaatttga agtaattttc
     3661 ttttagtaag aaaggtctgc tgaaagaaat agctcattat tgtctcctga atagaaaagg
     3721 gttacatttt tatatgaagc tgtattttta tataacatta ctggaggaga tattgtggat
     3781 acactggaac aaatcaaagg cggagtcgtc gtttcttgtc aagctttgga aaacgagccg
     3841 ctacacagtt cttttattat ggggagaatg gctttggctg caaaagaagg cggagcagtt
     3901 ggtattcgag caaatacgac agaagatatc aatgaaatca agcgaatcgt tgatttaccg
     3961 gtaataggga ttatcaaaag agattacgat gggtcagaag tgtttattac tgcgacagaa
     4021 aaagaagtgg aagaactttt ggagacgcaa gcagaaatca ttgcactgga tgcaacttct
     4081 cgtaaacgtc cgacagaaaa aacaactgct gaacttgtcc aaatgattca cgaacacggt
     4141 cggttagcta tggcagatat ttcgactttt gaggaagcgc ttcaagcgca agaagatggt
     4201 tttgatcttt tgtctacgac tttagcaggc tatacagaat attcacgaaa gacgcagaca
     4261 cctgattttg atttattgaa tcaaatcata gaacaagtcg atatcccagt aattatggaa
     4321 ggacatactg actctcctga acaagtagag aaagcatatg aattaggtgc tttttcagtc
     4381 gtagttggat ctattatcac ccgtccgcaa ttgatcacta aaagatatgt cgaagccgca
     4441 agtaaagcaa ataactaaaa aacacgcacg gcacagattc ttgacaaaaa agaaaaattg
     4501 atagatagtt taaagcagat tctaagaaga tgaaaaaagc attacttttc attttgtctt
     4561 caagatctgc tttttaacga attttataga aagaaggcga aaccatgttt gaacggagtc
     4621 cttcagaatt gacagaacgt gaaaattaca agttgctgat tggttcagtc atccctagac
     4681 cagtagcagt agtaacgact aaatcagcaa aagatatagt gaatatcgca cccttcagtt
     4741 acttcaatat cgtcagttcg aatcgaccca tcttatcctt ggcgatccag agaaaacaag
     4801 atgcagtaaa agacacagca aagaatattc ttgagacaag agaagctgtt atccatatcc
     4861 ttgatgagga caacgtagaa gaagtaaata aaacagcagc caatctgcct aatgaggaaa
     4921 gcgaactgta cctgactgac ttgactttaa ctgattcagt aatcatttca gtaccagcat
     4981 tgacagaagc aaaagttcgt tttgaaacat ccttatatga acatgtcgag atacggaata
     5041 aaaataaagt aacagctgat ttatttttgc tagaagtaca gaaatatcat ttaaatgaaa
     5101 cagtctatga tgataagaca ggaaggatcg atccaagaaa attgaatgct gtaagccgtt
     5161 tggcaggaaa tgattatgca gcaataggag aaattttcac gattgcacgt cccgaataaa
     5221 aaggagcact agttatgtta aaaataagct ataaaccatc gacagattca aaagaaatga
     5281 aaaaagaata tgaaacagtg aatgattttc tgcaagggca gtacttagaa gttccgccat
     5341 tgcaagacca ttttgtcgtt actactgtta ctttagatgg taaggaaata gaaatgccag
     5401 atcaaacgat tagcggattg tttaattact ttaacaaata aatatttatt ccgaaaagtg
     5461 aaaaaacata taaaaaaaca cataaaacta aaaaattatc aaagaatcct ggtgcaactg
     5521 aaagaatttg caacgggatt ctttcaactt atttgttatt tttttctaaa aaatattaaa
     5581 gattgttaaa tttcactatt tcatttcaaa ttccattgcc taatcattag gaaaagtcta
     5641 tcatgaactc gtaaatagaa tatagtaata accttgttag gtgaggctcc tatacaaaca
     5701 taggctgttg cccagaaacg tcgaaagtcg ccaatgggta aaacagaaac tgttggatta
     5761 aggctttttc caatagctaa gaatgttttc tttacgttgt atagtgctaa aactcggacg
     5821 atgagatgat gctaggtcag aattcttgtt cgaaaaggtt tctgaccttt tctttatatt
     5881 tttgtgactg acttatttga tgagacaaac tcgctcctca cctgacaagt ggattgctgt
     5941 gtgaggagga gatgcggtga aaaaagatcg attggtggat tttgggtcct tatttgactt
     6001 tgtccatgat cggtctttta gaagtataca gtgccagttc ttatcgttta ttgcaagccg
     6061 atgaaaatac aaaaagtcta ttattgcgtc aactaatttt catatttttg agttggagcg
     6121 tgatcttctt agctcgttca gtcaaactac actatttact tcaccctaaa atagcaggat
     6181 acggtttagc cttatcgatt ttctttttag tattagtaag aatagggata ttcggtgtca
     6241 ctgtcaacgg cgcacaacgt tggatctctc tgtttggcat tcaattccag ccttctgaac
     6301 tggcaaatct ttttttgatt ttttatttaa gctggttttt tcgtgacgga aatagtagcc
     6361 caaaagatct aaaaaaacca ttcctgatta cagtaggtat aacttttctg attttatttc
     6421 agccaaagat tgctggagca ttgatgatcc tttcgattgc gtgggtcata ttttgggcag
     6481 cggcggttcc atttaaaaaa gggatctatc taatcgttac tttttctgca ttgctgattg
     6541 gagcagcagg cggggtatta tatttaggaa ataaaggttg gcttccacag atgtttaatc
     6601 atgcttatga aaggatcgcg actttaagag attcttttat cgatagtcat ggtgcgggct
     6661 atcaaatgac ccattcgttt tatgcactat ataatggtgg catatttggg cggggattag
     6721 gaaatagtat cacaaaaaaa gggtatcttc cagagacaga aacagatttt attttttcga
     6781 ttattacaga agagcttggg ctaatcggtg cattgtgtgt gcttttctta ttgttctcat
     6841 tatgcatgag aatcttttgt ttaagcagtc gctgtaaaaa tcagcaagct ggattgtttc
     6901 ttttaggatt cggaactttg ctattcgttc agacgataat gaacgtggga agtatcgcgg
     6961 ggctgatgcc aatgactggt gtgccattgc catttgttag ctacggagga acaagttatc
     7021 tgatcctttc tttaggaatc ggaatcacgt tgaatatttc ttcaaagata caggccgaag
     7081 agttggcgct ctatcgacct gaaaaacaat aaagagaaac tgagaaactc tcagtttgaa
     7141 ttatcataaa gtacaatctt ttatttttca agcgagaggt tcttttagaa agaggaattc
     7201 gcaatgaaag tatctcaaaa aatcatatta
//
LOCUS       DAZKLA010000131         7218 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000131 DAZKLA010000000
VERSION     DAZKLA010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.133"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(229..906)
                     /gene="phoU"
                     /locus_tag="ACG85N_002450"
     CDS             complement(229..906)
                     /gene="phoU"
                     /locus_tag="ACG85N_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFQ0225165.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(919..1677)
                     /gene="pstB"
                     /locus_tag="ACG85N_002451"
     CDS             complement(919..1677)
                     /gene="pstB"
                     /locus_tag="ACG85N_002451"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0225166.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="ACG85N_002452"
     CDS             complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="ACG85N_002452"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0225167.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="ACG85N_002453"
     CDS             complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="ACG85N_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFQ0225168.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="ACG85N_002454"
     CDS             complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="ACG85N_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFQ0225169.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4433..5290)
                     /locus_tag="ACG85N_002455"
     CDS             complement(4433..5290)
                     /locus_tag="ACG85N_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFQ0225170.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5627..7075)
                     /gene="cls"
                     /locus_tag="ACG85N_002456"
     CDS             complement(5627..7075)
                     /gene="cls"
                     /locus_tag="ACG85N_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0225171.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         2094 a         1589 c         1255 g         2280 t
ORIGIN      
        1 ttgcattctt tatcctttct atgaaatgat gaaaagggtt tgtgacatgt tttgtcacaa
       61 accctttccc gaataaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaaa
      121 cgaaaaatat gaggagctat tttttgtttg actgttctta ttacttgatg tagaacagct
      181 ttttcacaac ctcttttcat aaacaaacgg cataatcaca gcttcagttt agaaaatctc
      241 attgtggttc gtatttaact cagtgatttt ccctgtagcc aagtagacga tccattcaca
      301 gatgtttgtc acatagtctc cgatccgttc tagataactg gcaacattca agtagtctgt
      361 tccactgatg actgtctcag gattttcctg catatttttg attgttgcat tatagattcg
      421 attgaaatag tcattgactc tttgatccat attggcgatc gtacgtgcgt ctttttcgtc
      481 tgttttgaca taagcaacta ggacgttgtc caccattttt ttcacataat cggacatatc
      541 ggagatctct ttttcgattt ccggtattct cgtctgccct tttacgcgaa tcgtggattt
      601 agcaacagac accgcatgat cggccatacg ttctaaatcc gagctagctt tcatgaccgt
      661 aatgatcata cgcaaatcag ttgtaactgg ctgttgcaat gcgatcattt caaaactttt
      721 cttctccaag cgtgtttcca tgttattgat atttatatcg ttttcgataa cttcttgggc
      781 taagatttta tcgtgtttga tgtatgcacg aactgacttg tgcacggcgc tgctcaccat
      841 catccccatt tcgtagaact gattatggag attcagcaat tcttcttcaa attgtgtacg
      901 tagcattccg tttcctcctt atccaaatcg acctgaaata taatcttctg tctcttgttt
      961 ttctggattc atgaatatct tcttcgtatc gttgtattcg atcaagtgac catctaaaaa
     1021 gaatgccgtc ttatctgaaa tacgtgaagc ttgagacata ttatgggtca cgatgatcat
     1081 cgtataccgc tctcttaatt ctaacagcat actttcgatt ttcccagtag aaacaggatc
     1141 aagcgcactt gttggttcat ccaataagat gatttccgga ttaactgcca aaacacgtgc
     1201 gatacataca cgctgctgct gcccgccgga taaagataaa gcacttttat gcaatttgtc
     1261 tttcacatca tcccaaacag acgctgcctt caggctttct tcaactactt gatcaaggac
     1321 ttttttatct ttttcccctt ttaatttcaa tccatagatg acattttcat agatagaaaa
     1381 aggaaatggg tttggctgct ggaataccat gccgatttct tttcttagtt caacaacatc
     1441 tgtttttgga ccatagatgt ctttgtcttt atataaaaca cttccggtga tcgttacatt
     1501 cggaatcagg tcattcatcc ggttcaaaga acgcagataa gtggattttc cgcaaccaga
     1561 aggaccgatc atcgcagtga tttccccttg attgaaactt agatcgatcc cttttagtgc
     1621 ttctttcttt ccataataca gatgaagatc tttagatgaa ataatttctt ttgtcatgtt
     1681 gaggcctccg tcttaaccaa aatgaccgga cacataatct tcggttgcct gaatttttgg
     1741 tctggtaaat atcttgttag ttttgtcata ttccattact tttcctagat agaaaaaggc
     1801 agtatagtcg ctgatacgag ctgcttgttg catgttatgc gtcacgatga tgatcgtata
     1861 gtcttctttc aagtttacaa gtgtttcttc aaccgttcct gtcgaaatag ggtccaaagc
     1921 acttgccggc tcgtctagca gcaagatatc aggtttcatc gcgatcgcac gagcaatgca
     1981 taaacgttgc tgttgcccgc cagataaagc caaggcactt ttgtccaagc tgtcttttac
     2041 ttggtcccac aaggcagctt gtttcaagct tgtttcaacg atttcatcta atttcttttt
     2101 gtttttttcg ccgtgctgtt tcaaagcaaa tgtaatattg tcataaatcg acttgctgaa
     2161 cggattagga cgttgaaaaa ccatgccaat ccgtttgcgc atctcataaa catccacttg
     2221 aggcgcatta atatctacgc cttgatacat gattttccca gtgattttcg tattagcgat
     2281 cccatcattc attcgattca acgaacgaag atacgtagat tttccgcagc cggaaggacc
     2341 aatcagtgca gtgatcttat ttttttcaaa ttccagatcc actcctttga ttgcttcatt
     2401 gtcgccatac cagacatgta tatcatccgt atatagtgct acttctttct cttttggcat
     2461 ggtgatgaca tttgtttcat tccagttata ttttttcatt ttgcttcttc tccttaggct
     2521 gaagtcattt ttcggtataa tcgtttgcca atcgcacgag cgccgaagtt gaacagtaaa
     2581 acagcaatga tcaacacggc agaagcgcca gccgaaacag aaacaccatc aggcatcgtg
     2641 ccttctgtat tgatcttcca aatatggaca gctaatgttt cagcttgacg gaaaatgctg
     2701 atcgggcttg aaacacttaa tggattccag ttcgtaaaat ctaacgcagg tgcactttgt
     2761 ccagccgtat agatcaatgc agctgcttca ccaaaaattc ttccagaact taggatcaca
     2821 ccagtcaaaa tccctggtgt cgcttctgga accaccactt tcatcactgt ttcccagcgt
     2881 gacaaaccta aggccaatcc tgcttctcgt tgtgtgtaat ggaccgcttg taaagactcc
     2941 tcaactgttc tagtcaaaag aggcaagttg aatacagtca aagccaatgc accagaaata
     3001 atcgagaaac catactggaa ttgaataacg aaaaccaaga aaccgaataa accgacaacg
     3061 acagaaggca acgagctcaa gatttcaatc gacgtgcgaa tcacatctgt tagccagttt
     3121 tttccagcat attctgaaag atagataccc gcacctaaag aaataggaaa actgatgatc
     3181 atcgtgatca aaagcaaata aatcgagtta aatagctgga tcccgattcc accaccttct
     3241 tggtaggctt tggaaggttc ggtcaagaag gaccatgaaa tgtgcggtat tccccgaact
     3301 aagatataaa gtagtaaagc tgctaaaatc aatacgatga tccccgaaac tatatataaa
     3361 acacctgtag caattttatc tgagcgtttt gcgttcatta tttcagctct cctttcttcc
     3421 caatcatacg gatcacgata ttaaagaata atgacatcaa tagcaagatc agcgctaatg
     3481 accataacac attattttca actgttccca tgattgtatt tccgattccc attgttaaga
     3541 tggacgtcaa tgtagaagca ggagtagtaa gtcctgttgg catcaatgct gcattaccga
     3601 tgaccatttg gatagctaag gcttcaccaa acgcacgagc cataccaaaa acgactgctg
     3661 ttaaaatccc gggcacggct gctcgtagca cgactttgta gattgtttgc catcttgtgg
     3721 caccaagcgc taatgaagct tcccgatagt gccgtggtac tgcctttaat gcatcaacag
     3781 tcatcgttgt gacagtcggc aagatcataa cgaacaacac aaatgtacca gctaagatac
     3841 caaaccctgt cccgccaaaa atacttctga caaaaggcac gataactgat aaaccgataa
     3901 aaccataaac gacagaagga atacctacaa gcagctcgat gaccggctgt aagatcttcg
     3961 ttccgttttt aggagagatc tccgtcatga atactgctgc accaatggca aatggcgtgg
     4021 caacgatagc agataaaaag gtcacaataa acgaacctgt gatcataggc aatgccccta
     4081 ccattggttt cccatttgct ccgatttggc ttggattcca atctgtacca aacagaaaat
     4141 caaatacatt gatcttatca gtaaaaaagg tggccaatcc tttactagct acaaaataaa
     4201 aaatagataa tacaaccaac acgatcagcg cgatacataa aaaactgatg aattttccac
     4261 gctgttccat ttttgaacgt tttgattttg ttaataatac ttttttcaca tcttccacag
     4321 atgactcccc cttaatcagg tttaacatac tcactcagtg tacaaattga acatcaattt
     4381 ggttttaata acatgtaaaa gtttagctaa gtttgtaaat tttgtgtaaa gattactctt
     4441 taacgatatt tccttgccaa tcacgttcga ctttcatatg agaaacaggg atataaccta
     4501 gttctccaac gatgttatct tgtacttcat cagataagat atagtctaaa aattcttttg
     4561 tcagtttttt tggatgatct cttgtgtaca tatgctcata tgcccaaatc gtccatttgt
     4621 tcgttgtcac attttcatct gtcggttcta caccgttaac attcaacgta tctacatcat
     4681 cggaaacata agaaaaagca acatagctga ttgcaccggg tgtgtcggca acgatctggc
     4741 gcaccattcc gcttgaatct tgttcttgtg cctgaacagc tgtttctccg tccaataccc
     4801 atttttcaaa cgtgctacgg gttccgcttc ctgaagcgcg gttcaatata actactggct
     4861 gatcttttcc accgacttca ctccagtttt tgatctcgcc tgtaaatagt tttttcaggt
     4921 tttccatact gatgtctttt acgccaatat ccttgttcac aataggagtg atcccaacaa
     4981 ctgctactcg atgatcgact aatgctgagg catcgatccc cgatttttct tccgcaaaca
     5041 aatcagagtt tccgatgtcg actgctcctg actgtacttg agacaaacca gttccgcttc
     5101 cgcccccttg tacattgatg aattttccag gattttctgt ctggtaagtt tcagcaactg
     5161 tttctactaa aggctgtaaa gcagaagagc caacagccgt aatggattct cctcgatcaa
     5221 tccactttga acagcctgat aaaagaatgg ctaatcctat tattggtaag aataatttaa
     5281 gttttttcat tagaaatctc ctttaatcct aaagtatgtt tattttatca ttttcattac
     5341 ttgatgaaaa cgaatcaaaa tcattaaata acaaaataaa ttctcataat tgtaaaaaaa
     5401 actatctcat taaaagtttc tatgctatac tggctttgaa atgtgtctct tttaatagca
     5461 tatattagct tatttcttgc tttccccaaa aaacaagaga taccccaaag ttaatttcgc
     5521 tcatgcgaga cgctttctcc atttctgacg tactctcaga aatggccccg ttagaaaagg
     5581 acacgagatc tccatctgtt gatggttctc atgtcctttt tatgttttaa aggagcggcg
     5641 aaatcaatct tgctaatcct tctttcatct tgatgattat tcctcggttt tcatacattt
     5701 cttttgtgag atgcgtagaa attttcgagt cttcaaaaaa agcttttctt acacgtgaag
     5761 ccattcgttc atcataaacc agtgtattga cctcaaagtc caagcgaaat gaacgaacat
     5821 cgatatttgc tgagccaact gaaacaatgc caccatcgat aatcatcgtc tttgcatgaa
     5881 taaatccatt ctcatatgtt tcgatccttg ctccatactc gatcagctct gcagcgaaag
     5941 aatacgttgc ccagtaaacg agaagatgat ctggtttgtt aggtatttgg atatgaactt
     6001 ttacgcctga aagaagagcc aacttcagtg cttcatgaat cgatccatcc ggtatgtaat
     6061 aaggcgtttg gatcaatatc tcccgtttgg ctaacgaaat cattttcaaa taagtcattt
     6121 tgatttgttc atgttcggta tctggtccgc ttgtcacgat ttgaacagct atatcaccag
     6181 tagtgtgaat ggacgggaaa aatttgggag aatagcctac ttcttttgta tgctgagaat
     6241 tccagtccat caaaaaacgg ttttgcaagg tatacaccgc ttctccgtaa atacgcaaat
     6301 gattgtctcg ccaataacca aatttcttta ccaatcctag atattcattt ccaacattga
     6361 acccacctgt atatccgatt ttcccatcaa taactacgat tttacgatga ttgcgataat
     6421 tgatccgagg attaatatac ggaacaaata gaggaaagaa aaatgcgact tctccgcctg
     6481 cttttttcaa attctgaaaa aagctggaag aaacttgtgt ggaaccccat gcatcaagaa
     6541 ggactctgac ttttacccct cgtcttgccg cctctgtcaa tgcatctcga acttcttcac
     6601 ctaatgcatc actgcgataa atataatact ggaagtggat atggtcttct gcctgcttga
     6661 tatcttcaat cagcgcatcg aatttttcac gaccatctgt aaataaagtg atttcatttt
     6721 tagtcgtata gagcgtactt tcaaataccg tgagcatata gatcaattgt ttcacatcta
     6781 cttgtccagt aggcggatga ggataaagcc ctcgcatcaa tgcctgtttg tcttcttcaa
     6841 tttccaggtt cttaccgatt ttcccttgga tcttcaaatc aaagattttc tctttggaaa
     6901 tccctcttcc taaaaaaata tataaaataa atcctacaac tggaatgaac atcaaaacga
     6961 gcagccatgc ccatgtttga gcagtttcct ttctttctcg aaaaacgatg ataaaggaaa
     7021 gcaaaatatt cataatcaaa aggatcgtaa aaaaattgtc aacgataatt ttcacggctt
     7081 cacctcttct tctaccaact taagaatacc caagttgttt tttaaagtaa agggaaaaaa
     7141 gccgcttcct tctattctcc tagccaataa aaaaagaaag ctggaacgaa actgtccaac
     7201 tttctcattt tatttata
//
LOCUS       DAZKLA010000132         7129 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000132 DAZKLA010000000
VERSION     DAZKLA010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.134"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..1062
                     /locus_tag="ACG85N_002457"
     CDS             52..1062
                     /locus_tag="ACG85N_002457"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0225172.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1131..2075)
                     /locus_tag="ACG85N_002458"
     CDS             complement(1131..2075)
                     /locus_tag="ACG85N_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0225173.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(2059..4779)
                     /locus_tag="ACG85N_002459"
     CDS             complement(2059..4779)
                     /locus_tag="ACG85N_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0225174.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(4776..5996)
                     /locus_tag="ACG85N_002460"
     CDS             complement(4776..5996)
                     /locus_tag="ACG85N_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0225175.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(6277..7122)
                     /locus_tag="ACG85N_002461"
     CDS             complement(6277..7122)
                     /locus_tag="ACG85N_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0225176.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT         2102 a         1357 c         1142 g         2528 t
ORIGIN      
        1 ggtatagtta atatataaaa ataataacta attacaggag tgcaaatttt aatgaaaaaa
       61 aaatcaatta ttttggcagc tactagtgcg ttggctgttt taactctagc agcttgttca
      121 ggcgatacta ataaagatat cgcaacgatg aaaggcggaa cgatcactgt atctgatttc
      181 tatgatgaag caaaacttga atcatcgaac caatccttag tacaacggat gatcatttat
      241 aaagtattca ataacaaata tggcgataaa gtaaccgata agcaagtgga tgcagaatac
      301 gacaaacaag cgaaatcatt aggtgatact tttgaatcac aattagaagc tgctggatat
      361 acgaaagata cttataaaga atatattcgt aacaatttag cttttgaagc tggcttgaaa
      421 gctcacgtag atattacaga tgacgatttg aaaactgctt ggaaatcttt ccatccagaa
      481 gtagaagcac aaatcatcaa attatcaagt gaagatgaag caaaagatgt gaaaaaatca
      541 gcggatgatg gagatgattt ctctaaatta gccaaagaca aatcaactga tacagagaca
      601 aaagaagatg gcggtaaagt taaattcgac tcaacgacaa caactattcc tgcagaagta
      661 aaagaagcag ctttcaaatt gaaagacggt gagatctccg atgttattac tacaacaaat
      721 cctacttctt atgcgacaga atactacgtt gtcaaaatgg tgaaaaacca aaataaaggc
      781 aatgacatgg ataaatacaa agatcaacta aaagatatcg ctactgaaac taaactaagt
      841 gacaatgcat tcacaactaa agtaatcggt gaagaattaa aagatgcaaa tgtaaaaatc
      901 aaagatgatg cctttgaaaa tgtgttgagt gcattcacaa caacttcatc aagtacgaaa
      961 gactcaagtg aaactacagc ttcaacaaag agttctgaca caaaatcaac tgattcaaca
     1021 aaagaatctt caactgaaga aacaactgat tcatcaaaat aaaagaaaac aaaaaaagcg
     1081 aagacttatg tctcgctttt tttgttttga atgaaaaaaa taaatttttg ctataaatct
     1141 tttggtacat agaaacttcg gttgtccata ccgaaaatcc gatcggtgta ttttcctggt
     1201 tctgtttgac gaatcgaagt caacatcatc tgcatcgaag catcgatatt gtcgatttga
     1261 tgtaatattt ctgcttccat tattcttgga cgtacaggtg agccatattc taataagcca
     1321 tggtgtgcta aaaccatatg acgtaatacg agaacatctt cgtcagcttc gctgattttc
     1381 aacgcaagac aggctttagt tatttcttcg tcaatcaata ccagatgacc gatcaaattt
     1441 cctgcaagcg tatattctgt cgacatagga ccggaaagtt caatcacctt tcctaaatca
     1501 tgcaaaatga taccagcata caacaatgct cgattcagct caggatattc atcggcgatt
     1561 gatttcccta agtgaagcat tgtcaaagta tgataagcta atccactggc aaatgcatga
     1621 tggtttcttt ttgctgccgg aaactcaaaa aattgttttt gatatttatt aagtagatag
     1681 cgaacaatac gattccaatg cgcattggtg atctcaaaca acaattgatt gatttcttct
     1741 tccatttctt ctttcttcaa aggggctctt tccatgtata aactaggctc tcctggctca
     1801 tctacccttg ttaagcgcat atgaagaatc tttacttgag ggtttccttg ataaacctct
     1861 cttttaccgt ttagaaaaac gactgtgccg gctgtaaatt tattgatttc ctcctccgaa
     1921 gcatcccagt atttaccatc gatagtacct gaagtatctt ggaaagtaaa agcaataaat
     1981 ttttttccgt ttttagcgat tcttacatct gcacttttga tcaggacaaa ttgttcaaac
     2041 tgctcatcga ttactaaatc acgaattttt ttcattattt gctccttcta attgttttac
     2101 gatttggttc atctcatggt aatagcttac catttcttta tcagaagaaa aacaaattat
     2161 ttgatacttt tctccaaatt tagctaaaag ttcagctaat tgatactttc gctgatgatc
     2221 ataatgaagc cagccatcat caatgatgat cgggcatatc gaacggttag cttctaatga
     2281 gaggtaagca aatctcatcg ccataatcag ctgatctttc gtacctgtag ataactcata
     2341 gatgggaaat tctgtatatc cgtctgttaa agtcagtgta tcatttttca gtaatatctt
     2401 ctgataattt ccttttgtta aaatacctaa ataatcacct gctgctctta acaactgggg
     2461 taattgtctt tcactcatct ctgttgaaag atcacctaag aatgtcccgg cgatttcaag
     2521 tgctccccat tcctccagta attcctgtaa ttttgctttt tgccggctag cttgttgata
     2581 aagttcgtcc aatgtgccgt ctctttgcaa ctcttctact tgaagttgga gtcgttgccg
     2641 attttcactt gcttcttttt cttcttgctg gagttgggat tgcttttctt tcagttggcg
     2701 cagtcttaca gataatttct cttttgtcag ctcttcagga aatagtggag taagcatttc
     2761 cgataattca ttgtatcttt tcatctgctg ttcggcttgt ttggcttttt gtaaaaagac
     2821 aggtacttcc gaaggataag ataagcctgc ttgagtcaat tgttcgctgt attcttgtat
     2881 ccatactttt tgtttctttt tcaattgatt gatttcttgc ttcaataaag tattttgctg
     2941 atagcttctg gcaaatttca tctgttccat ctcttgtata aaacgggaaa gcaataccat
     3001 tttttctgaa attgtcttat cttgtatcgg taaccattct ttaacaaact caaatttttg
     3061 gtcgatttgt cgtagttttt cttgtatttc ctgttctctt ttatgtttcc gctcaaatac
     3121 gccatataat ttctgataat ctatgacttc ctgtccatat tctttcatga gtttgagcgt
     3181 atccatcttt cctaagtgcc gatgatccgc aaaacgatat acttcttgct gaagaatctg
     3241 tttttcttct ccagttctct tttgtttttc ttctgcttca gctaattgag ctaaggctag
     3301 atcaagttgt cctagttttt cttgccacat tccttttatc tcagagaaat catttttagt
     3361 tttaattttt ggttgccttt tgtagaagaa atagccaatc atgcctgcac caattagtaa
     3421 aaggatcact ttcaagggca ttggtacaaa tatacctgca atgattattg ctccgccagc
     3481 catcagcata tacggtttag atgaagagac tggtgctatc tctgactgat tttgtaataa
     3541 tgctggatag gtctcttcca agtcattcac ttctgcttca atcatctctt gctgttcttt
     3601 agctatttgg agacgcacag aaatctggct ttctagatca gcaatccttc tttcttcagc
     3661 cagaagatgc ttccattctt cttcatataa aaattcttcc ggtaacggat ttttctgcca
     3721 atgccacctt tgttccaaaa tcttcaattc tgaatttgtt tctctagtct caatttgtaa
     3781 tctttgccat tcttcgttta attgttccgc tgtaaattga agttgctgga gatcttttag
     3841 ttcctgctcc tgctctaaat aaaaataata tctggctgac tgctgatcca tcccgctatg
     3901 cttttccagt gttgcttcta attcttccaa acgctcattt gtttgctgat accgtttgga
     3961 gaaagactcc agatctaaaa gaagttgctc atcaggaaga cctgtagtgc tgtttttttt
     4021 gagagattgc cattcttcat ataaagaaaa attaagttct tgctgtctgg ctaatgaaaa
     4081 ttgtttgttc gcctctttta attgtgccaa taactgctgc tgttgcgact cgtattcctg
     4141 ttcttgttgg atcaacttgc caaaataagc ttcctgttct tgtttttgat gaattttttc
     4201 ttgcagctct ttccattcat ttaatttttg attgattagt aatttttggc cttttttctt
     4261 gaataattgt tgtgctttct gataatattc ttgccgcttt tggaatactt gagaactgcc
     4321 cgtgatgcct aaagaaatca gcgcatcatg tagctctttt tctctcaaat gatctagttc
     4381 catcaactgc tcttgctgga aagtaaatac ctgctggaat aactctttcg tcaaaggtgc
     4441 gatcagtttt tctagtaact catcacttcc cacttgatcc cccagccaca ccttcgattt
     4501 tccgcgattt tgctgcttgt agcgctcgac agcaaattca ccatatacag gatgtttaag
     4561 ccatattttg ccgccatagg ctgagccgtc ttttggtgta taatcctttt ttttacggcc
     4621 ttttgaaggg aaaccaaata atattgcttg aataaattga taaatcgttg attttcctgc
     4681 ttcgtttccc ccatagatca gctgattgcc tgaataaaag tcaaaacttt tttgacgcca
     4741 ttttccaaaa gcagcaatct cgatccttaa aatcttcatt cctgatcctc ctcaaataaa
     4801 aaatcttgat taagcagctg cttggctttt tcaagtgttt cttcttgata ttccggcagt
     4861 tcgctcaata ttctagccgc ttcttgatga ctatatactt catttaaaat ttgttggaaa
     4921 tcttgaggtg tctgatagta ttgaaataac tgttccatca atttagcaga agcagcaagt
     4981 ggggtcttcg tataaaaagt attctctatg agagaaatac gccaaataaa gaaatcattt
     5041 atcctatctg agaattcctc taacaaatag tctaatagct ctcctgattg tatccgttcc
     5101 aaaacatctg tattgagatg gtcataatct tttaatttta tctcaattaa actaaatcca
     5161 tcctctactt ttgaaagctg ctgacgaatg tgaacaatga cctcttttgt cgttcttgct
     5221 ttcctcaatg atatttcctt tgtcttccaa tagacctctg ctactttaat cggatgtatt
     5281 tggcaacttc ctttacttaa ttcaactagt agaatagatg tagaagcttg ttccttttta
     5341 gtatgacctt gaggcgcacc tggataaaca attgtttgct tttcatttaa gaccgttggc
     5401 acatggatat gccctaaagc ccaataatcg tatccctttt cttgcatctt tgagggttgg
     5461 aaaggagcat agtttccttt ttgagagacg ccaggttccc cgtggtacaa cccaatatgg
     5521 taatctgtga gttctctaga aggaaattcc atcactttgt ctttttgaat ccacggttga
     5581 cgataactaa atccactgag agagacagtt tctccacttt ttatcgttat tttttttgtc
     5641 tctaccgttt cggatgtaaa taaatggaca tttttaggaa aggagaacca ataccgctct
     5701 ttttgataga aatcatgatt tccgaatatt ataaatacag gtatatggtt cttttctaac
     5761 cgtttcatct gttctgaaaa atgtttctgt atttttaatg aaggacgatt ttgatgaaaa
     5821 ttatctcctg caagtaaaac aaaatcaacg ttatttatta ttgcctgttc aatgatattt
     5881 gataaaacgg tcagattcgt cttcaatagt ttttcctgta cagttttatc aagagttgtc
     5941 agtccttcaa atgaccgatc catatgtaaa tctgccgcat ggataaattt caccattaaa
     6001 aatttcctcc tttttttctt ttatcatacc attttcaatg ttttttttca tcaataaccg
     6061 aaaatttgtt cgtattatgc aaagaaatta cgataaatgg ccgacttcaa aaaataagtt
     6121 tattcgaaaa aaagattgtg acatgatttt tgtcacaatc ctaaatatct atgttatatt
     6181 cgcttcgttt gacactgaag aagtactatt tgtactggaa tttatgctcc atttttccgt
     6241 atttagaatt ctatgttcta tactacctta attagtttat atggtaagaa ttctttcaaa
     6301 tgcttgagat actccgtctt tatcatttga aacagtgaca taatcagctt ttttcatgat
     6361 ttcttgatga ctatttccca ttgcaaaact aatatctgca ctttcaaaca tagataagtc
     6421 atttaaacta tctccgatcg cgattctcaa aggtttcttt aaattgtaga ttgattccaa
     6481 acgatcaaac accacacctt tagatacatt ttgaggtacg atttcaatat ttacaggtgc
     6541 cgattcagta acagacacat ttagcatctt tttcaaatgg ttaaatatat tagctttcaa
     6601 ttgacgatta ggaataaaaa taaaaaattt gtaaatgctg atagcatcct tttctataat
     6661 ttcctcaata ttattataag ggattgaaca aatagatttt tcatactcaa gctcatagtt
     6721 tagctctttg atagaaaatt cactccctac ctcgtgaaaa atttttttca ttttattttt
     6781 ataattctga gattcataac ttccgagatt tgtatatatt ttggttggaa atttcgatag
     6841 gacagaatca atttttttta catcattttt atcgaaggaa gactcaaata tctttttttc
     6901 tccatcaaat ccaacagaac catttaaccc tactgctggt atccttaaac cagatttttt
     6961 gagaatatta ttaatgtctt ctacttctct tccagaacaa atgaatggaa aacagtcttc
     7021 cggtaaatgt ttcattgttc ttgcattttt ttgactaatg ctctggtcag aattcaaaag
     7081 agtaccgtct aaatctatac caattagttt catttcatcc atgtctcct
//
LOCUS       DAZKLA010000133         6800 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000133 DAZKLA010000000
VERSION     DAZKLA010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.135"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..773)
                     /locus_tag="ACG85N_002462"
     CDS             complement(<1..773)
                     /locus_tag="ACG85N_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0225177.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLI"
     gene            complement(893..1540)
                     /locus_tag="ACG85N_002463"
     CDS             complement(893..1540)
                     /locus_tag="ACG85N_002463"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFQ0225178.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(1624..3051)
                     /gene="uxaC"
                     /locus_tag="ACG85N_002464"
     CDS             complement(1624..3051)
                     /gene="uxaC"
                     /locus_tag="ACG85N_002464"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0225179.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(3125..4204)
                     /gene="uxuA"
                     /locus_tag="ACG85N_002465"
     CDS             complement(3125..4204)
                     /gene="uxuA"
                     /locus_tag="ACG85N_002465"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0225180.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(4207..5838)
                     /locus_tag="ACG85N_002466"
     CDS             complement(4207..5838)
                     /locus_tag="ACG85N_002466"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0225181.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            6142..>6800
                     /locus_tag="ACG85N_002467"
     CDS             6142..>6800
                     /locus_tag="ACG85N_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0225182.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     Y"
BASE COUNT         2196 a         1362 c          938 g         2304 t
ORIGIN      
        1 ataattaata attgagcaag gagtaagaaa atagccgcac caattataga aagtaatgcc
       61 ctatttttaa ctaacccttt taaagatttt aacaatgaaa ctttgtattg agattggtca
      121 attcttactc gttcagtagt tagtttataa catattaaat aacaaataag tgcacatatc
      181 gaaaatattc ccgcaacaat tgtaaaagtt gatccaccac gaactatttg agcgcctgaa
      241 tcatctcttg tataaataaa taatggtgtt aatacaccaa tgaccaaact tgctaaagta
      301 gctccaacat ttcggaatgt cgatagcatt gttctttctt ctgtaataga agtaatagct
      361 gatgccattg atccataagg aatatttata gatgtgtaaa aaatactccc ccataatata
      421 tacgtaacat acatgtatac taccttaacg ctcatagaag ctgaaatcat agaagtttga
      481 tacattaaaa aacttgctaa agcgactgga ccactcattc ttaaaatcca cggtttaaac
      541 ttaccctgct tagttgcttt cgatctatca acaatacgcc ccatagttac atcagtaact
      601 gcatcgataa atcttgctac taaaaataat gttccaacgg cagctccgct tatccccaat
      661 acttttgtat aaaagatcat taaaaaagaa ctagcaaaaa tgaaagtaaa atcgtttcca
      721 aaatctccaa acatatatcc tattttgtct cttataccaa attttctttc catacaattc
      781 tctcccttct gttgttaagc gttttcattc tgatgaaagt acgtactata ctctgaagcg
      841 taaagagtca gtatttattt tttacatttc agagtattat ttgttttttc tcctaataat
      901 tctttatctc ataaaactta tctatatatt tactagctac tcgacttact tcttcaaaat
      961 cacctttttc aacagaagcc aataaattcc cacctacacc aactgcaaca actcctactt
     1021 caaaccaatc ttctaagttc tctatactta ctccgccagt aggcattata tttacgtgtg
     1081 gtaatggagc ttgataggct tttacaatgg atggaccaaa agcagaacct gggaatagtt
     1141 taatgatatc tacgccagca gtaagtgcct cctttatctc agtaattgtc atacaacctg
     1201 gtaaataagg aatttgatat aaattacaaa tctcagctgt ttgtcgatca aaagatggac
     1261 taacgatata ttctgcccca gccataattg cgatacgagc agtaaacgca tctaaaaccg
     1321 taccggctcc gattattaca tctttttgat cagcatattt tttagcaatt tctttgatta
     1381 tttcttcagc attaggaact gtaaaagtta gttcaatccc tttaattcca cccgcaataa
     1441 ttgaatttat tgctttaatg gcctcatcct tatttttccc acgtacaacc gcaatgactc
     1501 cagccgcttc taacttactt aaaatttcta cttttttcat atctatctcc ttcctttatg
     1561 gatacatact ttgtctagct gtactaactt acaaagaata aatttaagct tttatttcaa
     1621 gtattatttt ctacttccga tttcaaaaaa attaaaataa ttatacgcat tacgataaca
     1681 aatattctcc actatctgac taagatacat ttcatcatct ggaattcggc ctgctttaac
     1741 ccactcgctt agtaattgac ataatactcg acgaaaatac tcatggcgag gatagcttag
     1801 aaaacttcga gaatcagtca acatacctgt aaaattcgac aacaaacttt gttgtgccat
     1861 cgtagtcaac tgttgacgca ttccttcagc tgtatcattg aaccaccaag cacatcccaa
     1921 ttggattttt tgaatcatac ccccttggaa gctttgcatt gctgttgcta gtggaagcca
     1981 atcagttgga tttaatgaat ataaaattgt ttttggtaag cacccactct tttcagcggt
     2041 atcgaacaat gctacaacat tttgtgtcaa actagcttga tcccccatcg aatcaccacc
     2101 gacatctact ccaatttgtt cctttaatat acttgaatta tttcttaaag catttaaatg
     2161 catttgcatc gtccaaccat atcttgtata ctcagacatc aaatgtaatt gaatacctgt
     2221 aacaaattgt gcaatttctg ataaatggaa atccgttttt cctgctaaag cttttattaa
     2281 cagattgtcg agtatttttt catctgctat ttcataaaaa taagtattca aaccgtgatc
     2341 tgctaatcga cagccaattt gatgaaaatg atccactcgt tggctcaatg cttgcttcaa
     2401 cccattaaag tctttgattg gtatagccgc tgtttgacta agttttaaaa tatagttcga
     2461 aaagcctgat gatttgatac tccagacctt atctggacgg aaagtaggta ataccttgaa
     2521 agaattatcc tgtttagaaa gctcagtatg ataccacaag tcatctgtag gatcatccgt
     2581 tgtacagatg gctttcacat ccatcatctc caacaattct ttcgggcgat aggaatccgt
     2641 ttgaattttt tcgttagcac gtcgccaaat ttctggtgca ttcttctcag aaattgtcaa
     2701 atcaatatca aagtaacgtt gtaattccaa atgagaccat tcaaaaatag gattacctgg
     2761 cgccttctcc actgttttta caaaagctaa aaatttttca taatcatcac ctggaccact
     2821 gatcaactct tcttctatac cattagctcg catcaatcgc catttataat gatctccagc
     2881 tcctttatca tttaaccata cctgagtcaa attcttgtat tgcttatttt ctagcaaata
     2941 tgctgggtct aaatgacaat gatagtcgat aattggttgg tgttttgcaa atttatgaaa
     3001 aagtctttta gctatttcat tctctaataa aaagttctta tctaaaaaca tatttaaaca
     3061 ctcctttcct tccgttagtt tttagaagag aaaatttttc ctctaaataa actcccttat
     3121 ataattattt actcgccccc ttttcaagcg cttcccaaat accatttaaa taaactgcac
     3181 ctagagctcg gtcatataat ccataaccag gtctaccggt ttctccccaa atcatccgac
     3241 catgatctgg acgaatatag ccattaaatc cattatcgta cattgttttt aatatcttat
     3301 acatatctag agaaccatat tctgatttat gcggcgcttc gtaaaaatcg agtccattgt
     3361 cacttaaata tttgatatta cgaacatgtg caaaaggaat ccgatcccgt tgacaaaatt
     3421 cagatagaat ttcatagaca tcattatctg gtcgttctgc aatacttcca gtacataatg
     3481 taataccatt agaaggagaa tccactacat tacacaacca atctaaatcg tcacgatttt
     3541 taacaatacg aggtaatccg aaaatggaat atggtggatc atctgggtga actgccattt
     3601 taatcccaac ttcttcacat gtcgggataa ttgctttgag aaaatatgca aagttctctc
     3661 gtaatttctc ttcatcaact tctttatagg cttctaataa ttgcttaatt tgtgataacc
     3721 gatgaggttc ccaacccggc aattcaaaat tattagtaga attttctact tctctaataa
     3781 tttcctccgg atcagtaggg atattttttg aaatataggc taacgtagaa gacccatcat
     3841 ctaaacgata atccaaatcc gattttaccc aatcaaatac tggcataaaa ttataacaga
     3901 taacctcaat cccaaattct gccaaattgc gaatcgtctg tttataattc tcaatgtatt
     3961 gatctcgtgt tggtaaacca attttgatat catcatgaac attaacactc tcaataactt
     4021 tcaaggtaag tccattggct tcgacttcat cttttaaaga tttaattcgt tctctcggcc
     4081 aaacctctcc ggctggtata tccattaatg ttccaacaat tcctttcatt gctgggatct
     4141 gacgaatttc ttgtagtgag attttttctt catttgcccc ataccatcta aaagtcattt
     4201 ccatagttac tttcctccaa cttctgttat atatttattg attgttttac gaactgcttg
     4261 aggacctact agcatttcgc aaaagatttt ttcagttttc tttcctaccc caacttgata
     4321 taaatctaaa ccaaagattt ctttattttc taaaatttcc tgtaccgcac aatgaatctt
     4381 tgaaggatct tgttcaccta atttcaatac tgataattta tcctgcaact ctaccaataa
     4441 cggatcagga cttggcacaa aattttcccc ttgatcatcg actgccagta aataacgtaa
     4501 ccaagcagca atagcgatag gaataaatgt tagattttct gcatttccgt ctttttcgac
     4561 atacttttta attgtttcac catagcgaat cgctactttt tgagaagtat ctgttgcaat
     4621 tcgttgtggt gtatctggaa tatagggatt aggcaaacgt ttattaatta cttcatcaat
     4681 aaattgtttt ggatcaataa tgcctggatt ttcaactact ggtaaacctt ctaaataacc
     4741 tattcctttg attaaaccaa ccaaatcatc atcctgcatc tctgctgaaa ttgattgata
     4801 tcctagcaga caacctgtta ctgccaaagc agtatgaagt ggattcaagc ttgtcgtaac
     4861 cttcatgatg tctgctttat caaccgtctc tctatctgtc ataatcacac cagccttttc
     4921 caatttaggc cggccatttg gaaaactatc ttcaataact aaataatgaa ctacctcagt
     4981 attagtgaaa gcagcaatat tggtatgttt ttttgtgtga atgatatcta aatcctcgag
     5041 ccccatcatc aatagatctt cctttacatt ctctgaagga tttggcgtaa ttcgatcaat
     5101 catactccaa ggaaaagtca ctttttcttt atcctttaaa taagcaataa atttttcaga
     5161 aacatatcct tctttttgcc aagcttctgc aatcgtaaaa atactatctt gaaaacgttg
     5221 accattctgt gagaaattat cagtagaaac catcgcaatc ggcaactctc cagcattaaa
     5281 tctgctatat aacaaggatg ttacaatact catcgtatgt tgaggattgc taggtccaga
     5341 tttgatatca gctttgacta ccgaaaagaa ttgcccttta gaatctttta aactgtaacc
     5401 tttttcagta atagtaaacg ttgtaaactg taatttagga tcttcaaaat atttaaccac
     5461 tctttgatag tccgctatat tcttcggatt gcaatataaa ctatcagccg ttactgccaa
     5521 aagtcttttt ttgagttcac catctccact cataacaatt tccaaaatgt cattgttata
     5581 gtcatgataa gctttttcca ctacttcttc atcaaatgtt tcacacacaa ctactcctgt
     5641 ttctaattca ttttgatctg ctaagcattg agcaatttct gcatgaaatc ctcgataaag
     5701 atttcctcca ccaaaatgaa gccatactgg atgttgctgg ccttcttgct taacttcttt
     5761 gacatcaaaa cacggcagta gaatctgcat ttcattgaat tcttcgatag aattttgcca
     5821 atttttaatc gaaaccataa ttaacctcct caaactgata tgttagtttt gatacatttt
     5881 atattttaaa acccattgta accgttgtca atataaaaag ggaataaact tgcttttttc
     5941 taccatatca gaatgattgt attttttatt cagaaaaata aaatactcct tctttttgga
     6001 ctgattttat cgaaacttat tttttctttt tttcatgtat tcttttattt ttataactag
     6061 tgtattactt caaaatttga tattatatgg catgaacaga attaaaatat cagttaaaat
     6121 aaaagttaag gagcattgtg tatgacaatt aaaactagtc tccaaaatca agcttaccaa
     6181 tctattcgcc gacaaattat ctatgctgac ttagaacctg gaaaaaaagt atcagaaaaa
     6241 aaattagaag aaacactttc tattggtcgt acaccaatac gtgaagcact gattcaactc
     6301 cgacaacaag gtcttgttga tacaattcct caaagcggaa attatatctc attaattgat
     6361 ttagattcgg ccaaaaatgc tcgatttgtc agagaacatt tggaacgaca aattatgtta
     6421 gagtgttgtg ccaaaatgaa taaccaaaaa aaacggattt tagaaactat aatttcagaa
     6481 caagaaaaag ctgttagtca aaaaaatgct cgtagttttt ttcataatga taatttattc
     6541 cataaagctt gttttgaaat tgcagagcga gaagaagtat ggcaatggtt agaaaataac
     6601 aatacacact tagaacgatt tcgatggcta cgagtaaaaa cggaaggatt aaagtgggaa
     6661 aaaattatgg aacaacatta tcaactattt gaggcattaa caaaacaaga tccagaagaa
     6721 gctaactttc taacatcgtt gcatcttcat ttaatgctta atgagcaaaa tattgtttta
     6781 gctactcatc caaaatattt
//
LOCUS       DAZKLA010000134         6720 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000134 DAZKLA010000000
VERSION     DAZKLA010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.136"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            10..900
                     /locus_tag="ACG85N_002468"
     CDS             10..900
                     /locus_tag="ACG85N_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFQ0225183.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(973..1797)
                     /gene="nadE"
                     /locus_tag="ACG85N_002469"
     CDS             complement(973..1797)
                     /gene="nadE"
                     /locus_tag="ACG85N_002469"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFQ0225184.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1809..3281)
                     /locus_tag="ACG85N_002470"
     CDS             complement(1809..3281)
                     /locus_tag="ACG85N_002470"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFQ0225185.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3918..4280)
                     /locus_tag="ACG85N_002471"
     CDS             complement(3918..4280)
                     /locus_tag="ACG85N_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0225186.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4503..5255
                     /locus_tag="ACG85N_002472"
     CDS             4503..5255
                     /locus_tag="ACG85N_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0225187.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5395..6318)
                     /locus_tag="ACG85N_002473"
     CDS             complement(5395..6318)
                     /locus_tag="ACG85N_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0225188.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6333..6605)
                     /locus_tag="ACG85N_002474"
     CDS             complement(6333..6605)
                     /locus_tag="ACG85N_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225189.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2047 a         1347 c         1152 g         2174 t
ORIGIN      
        1 gtgatatata tgcaactaca aataaacgaa gaatccttac cggtatatga agcattagct
       61 agtaaaacaa ggatcaagat catccagcta ctttccaaaa aaaagatgaa tgtcaaagat
      121 ttggcaaaag aacttggagt gagcagtgct atcacgacga tgcacgtgaa aaagctagag
      181 gaagcaaaca tcatcaaaac agaaaaagta ggacaacaaa aaatttcgag ccttcgtgtc
      241 gataaaatcg atattagttt tccagaaaaa atattcaatg cttttgatac gaaagaaaca
      301 tcgataccta ttggtcacta tacaaattat gcaattgaac caacatgcgg tttagctacg
      361 atccatgatt ttatcgggaa agtggatgaa ccacgctatt ttatggatcc tcgaaggatg
      421 gatgcccgca ttctctggtt taccagcgga tttgtcgagt atcaggcacc taatttttta
      481 aatacagagg atacattgga gatgttggaa gtttctgtgg aaatttcttc tgaatttcct
      541 ttttcaaatg acaattggcc ttcagatatc actttcagcc taaatggtgt ggagctagga
      601 acatggacga gccctggaga ttttgctgat atccgcggta aatatacgcc tgattggtat
      661 ccagataatc ttaatcaata cggcttgctg aagacgatcc gaattaccaa acacttgacg
      721 aatatgaatg gagaaccctt gtcaaacatc acgatcaacg acatccctaa agagcaagac
      781 acttggcatt tgcgaatcga agtgaaagac gatgccaaac atgtgggggg ctgtacttta
      841 tttggtaaag gattcggtaa ttacgatcag gatatcaaat taaaagttta ctacagctga
      901 tatttttctt ttctatttta taaaacaaaa acagccaatc cctatgaagg gacggctgtt
      961 tttgctttat tgttatttcc agaaatcatc caaaacggaa attggcaaat gtcgtttatg
     1021 ttgtgtcttg ttccaccatg cttcgatttt ttcttgtgct tgagcgggaa cttcttttcc
     1081 ttctagataa tcatcaattt cattatatgt gacacctagt gcgacttcat ctgcaatcat
     1141 tggtttccca tcttcaagat ctgcagtcgg aatttttgtg tataattttt ccggtgcatt
     1201 caatgcttga agcaattgtt tcccttgtcg tttgtccaaa cggaataatg gcaaaatgtc
     1261 ggctccacca tcaccaaatt tcgtgaagaa tccagtaatg ttttctgcag catgatctgt
     1321 ccctaatacg gctcctgctc tttctccagc tacggcatat tgcgtaatca tacgttgacg
     1381 tgctttgata tttcctttgt taaaatcgct gattcccaca cctgcttcgc ttaatacttg
     1441 tacttgtgca tcaacagctg gtttgatatt tactcgcaga ctaacatcgg gctggataaa
     1501 gtcaagagca gcttttgcat cttcttcatc tgcttgttca ccataaggaa gacgaacagc
     1561 gataaattga tagttttcat cttttgtttc ttcacgcatt tcttccatcg ttagttgcgc
     1621 caaacggcct gctaaagtag aatcttggcc gccgctgata cctagtacaa aagttttaag
     1681 aaacggatat tttttcaaat atgctttcat aaagtcaata cttttacgta tttcttcttt
     1741 tggatcaatt gttggtttga cacctaattc attgataatt tctgtctgca ggctcattta
     1801 ttctcctcct atgcttctgt atgaagttct gctacgcttt ttttcattcg ttccatgata
     1861 gccattttat ggttccagca ttcagtagac agatcgactg ggtatttttg cggattcaag
     1921 tctcgtttat actcttccca tagtgaatct agattttctt ttgtatattc tttgatttcg
     1981 tttagtgcag gtaattcgta aactcttttt ccttcgataa agatatcttg taaaactggg
     2041 cgagctgtaa agtctcgaac agttttattg ataaacgtat gcactggatg gaacatgaag
     2101 atagcttctt cttctcgagg gtcttcatcc cataatgtca cgtagtctcc ttcagatttc
     2161 ccatcaaaat ttcgagtgat gcgccacact tgttttttcc ctggagtagt tactttttct
     2221 gcattgcttg ataacttgat cgtatccatc atttttcctt catcatcttc gatcgatact
     2281 agcttgaata ctgcaccaag ggctggctga tcataggctg tgatcaattt tgtcccaaca
     2341 ccccatacat caattttagc tttttgcatc tttaaattca aaatcgttgc ttcatccaag
     2401 tcattcgaag catagatttt tgcttccgta aatccggctg catctaattg ttcccgaaca
     2461 cgcttagata tataggccat atctccactg tcgatgcgta cgccttggaa gttgattttg
     2521 tcacctaatt ctttcgccac gcgaatcgca cttggcacac ctgatttcaa cgtatcataa
     2581 gtgtcgacta agaacacgca gtctttatgg gttttagcgt aggccataaa tgcttcataa
     2641 tcatttccat aagattggac taacgaatga gcatgggtcc cgctagctgg tatcccaaat
     2701 atcttcccag cacgtacatt gcttgttgca tctgctccgc cgatatatgc cgctctagtt
     2761 ccccagactg ctgcatccag ttcttgtgcc cgtctagttc caaattccaa tagtggatct
     2821 tctccgatga cagatttgat acgagctgct tttgtcgcga tcaacgtttg gaaattgacc
     2881 atattcaaca gtgctgtttc tactaattgg cagtgcgcta aaggcccttc cacttggatc
     2941 aatggttcat ttgcaaaaac tagttctcct tcacgagcag aacgaactgt tgctttaaat
     3001 tcaaaatttt gtaaatactc caaaaaacct tcaggataga cttctaatcc acgcaagtaa
     3061 gcaatatcgg aatcattgaa cgtcagattc tctaaataat tgactaaccg ttccaatcct
     3121 gcaaaaaccg cgtatccgtg gttaaaaggc atttctcgaa agtaacattc aaaaacagca
     3181 tgtaaatcag ctcgacctaa ttcccagtat gtctgcatca tattgatttg atatagatcc
     3241 gtatgcagcg tcaaactgtc gtctgggtaa gtattattca tcttgtatta ctcctcttgg
     3301 caagttttca tttcctaccc attataacaa aaaaaattaa taggaaaaga aatcttccat
     3361 tttttctgaa aaagcacatg ttttacggat atttcttgct aaaaatgaac tccatctgct
     3421 aaaactatcc tttcttattg gtatttaacc taaacgagaa agaatactta ttttattcaa
     3481 tgtatccgag acacgaagaa attagcgttt tctatttttc ttacttgata aaataagtag
     3541 attttcctac tacaatcgta gccgttaaga taccgatgat tgaacaaata taaccaaatg
     3601 tctctacaac ataatcaatg gcaaaagttt ttgaagcaat aatacgtctt tccttttttg
     3661 cttcttatcc ccaaagaaac ggatataact tattatgtat tgaatttttt tggaaaggcg
     3721 aattatgaca agatatacct tgcgttgaaa aaactcgcgg aaaaaatatc acacgttttt
     3781 aaaaagatac aaagtaagaa agcagctgtc aatagaatgg aaacaaaaat ggcgagacat
     3841 caatcagtct cgccttgttt tttattgtat acattagata tggagcaggt taccataaag
     3901 atgattatta gtcgttatta aatagaatcc ttcgtatttt tttgttctat cactacaata
     3961 tctttatttg gacgtttgaa ttgtattccg gcacgttcta gctcctgttt cactcctaca
     4021 tggactttga catcttctcg tgtcattttt aaaacgtgat gaatcgcata gtcgatttct
     4081 tgccttgtga tttttttctt cgtattgatc atcacgactt gctttttatc atatgtgtct
     4141 accaatagca gttgtgttcg atctaacgaa aataatttgt aataattaat agccgtattc
     4201 ccaaaaaaga attccactac tttaggataa aatgtcttaa tatttaaatt actattcaca
     4261 ggtgtttcga ttagcttcat ctcattcctc ttttctatat agtcgattta gatccaatct
     4321 taagttatgt taccatcttt cctagcatta aggaatcggt tacaattgaa cctttaaaga
     4381 caaaaggatt taccttttat cacttaatag tataacataa tttcatgaga aaaaaataca
     4441 attttttctt gaccatctcg ttacgtaaag gattatgctt acatttgtca ggaggaaaaa
     4501 taatggagta cacaataaag aaaatgtcag agatatcagg agtcagtccg agaaccttgc
     4561 gcttttatga tgagattgga ctgctaaaac cagctaggat caattcttca ggttatcgga
     4621 tttatggcaa aaaagaagtc gatcgtctgc aacacatctt attttatcga acaatggcat
     4681 tcaagttgga tgatatccaa gaggtcttag acaatccatc ttttgaccac cagaaagctt
     4741 taatcaagca tcagcaaatg ttgctggaaa aacgggcaca aatcgatacc ctcttgacaa
     4801 ctgtccaaca gacacttgat atgtacgaag gaggaagaaa aatgagtgac aaagaaaaat
     4861 ttgaagggtt taaacaacaa aagttgaaag aaaatgaaga atcctatgga caagagattc
     4921 gacaaaaata cggaaaaaaa aaggttacag catccaatga aaaatggatg aatatggata
     4981 aagaaacgta tgaagaaatg caaactgttg aaaagcagat gttatttgat ctcactcttt
     5041 acttaaaaga accagaagat gaaagcttag cagaccgtat cttccaagct cataagaagt
     5101 ggttatctta cagctggcct gaatatcagc caaaagcgca taaaggacta ggaatgatgt
     5161 acgaagccga tgaacggttc actgcatatt atgacgagcg aagtggtcat ggtgcagcaa
     5221 aagctttaaa tgaaattatc caaaaaaagg cgtaatcttt tacatgatcc aacaaaaaag
     5281 tacacgtaaa atgacctaat aacaaaacag cagacaccca agttagattt ttatgctaac
     5341 ttttggtgtc tgctccattc tttcacaagt tttttcatcc tactttaaat atattcatcg
     5401 ttgaataaac gtataaggta ttttcacttt atcctttttt tctgtaagaa acaattgaaa
     5461 tgccatttct ccgcaagctt ccagatggtg gtcgatggtt gggaaaccaa gaagctcgct
     5521 gatcagcaaa ttatcgcgtc caatcaccac tggtgtctta tcttgcagat agctttgcaa
     5581 gatgcctgct gcaaccatat ccgaatttgt aaagacacca tcaactccaa gctctttaaa
     5641 aaagaagcct gcttgttttc ctttctctgc atctatacaa tcccaaaaaa caagtgattc
     5701 atcaaaagat ggaaaaagtt gatgacatag ctgcaaagtg atttttgagt tataacttag
     5761 cctgccactt cgccccaacg tgatgcccaa ttgctttata ccttgctgct tcaaataagt
     5821 aagaccttct gtaattgatt gttctcgatc aataaagaca cagcttgcct gttttccctt
     5881 gatttcttca caaaaaacta gaggtccata tttttgatat gccaataaat catctagtgt
     5941 attggcacga gacgtaatga tcaatccatc gaaagcttta gctgcaaatt cttcaagata
     6001 ccttcgctct ttttctttct gatagcccgt cggtaaaagt gtcactttgt aatcataatc
     6061 aaacgctgct tcaatgatcc cactgatcaa ctgatcaaaa tacggatgat tggtataagg
     6121 cagaatcact ccaatgcttt tcgtttttcc ataacttaac tgtctggcat ttgcattagg
     6181 gatataatcg acttctttca tgaccgcaag tatttctttc cttttttcct catctacata
     6241 tggatgtcca ttgattacac gtgaaatagt agaaactgaa taacctgtga gctttgcgat
     6301 atctcgaatg tttgccactg ctgcttcgcc acctatacaa aaagattttc ttgtaaatag
     6361 tctaccacag ctggaccgca ttcaaaaaac tgatacattt ttccttcatg cgaaaaactg
     6421 atccgctgcc cgccccaata tatatctttt gtcaaatttt cgatttcatt cattggtatt
     6481 tcttttacta tattcctatt caacccttga aaactgtaca acgttaatac ggatcccttg
     6541 aactcgacaa acgcataaaa ataattgttc gttgtgccat ctgttatctc tacataagta
     6601 ttcattgcaa gccctccttt tgatactttg agtatagtgt atgaaacgcg tctcatagca
     6661 aggagtttgt tttgccaaag aaaagtttta ttcctctttc attattacca aagtctctta
//
LOCUS       DAZKLA010000135         6498 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000135 DAZKLA010000000
VERSION     DAZKLA010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.137"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..1711
                     /locus_tag="ACG85N_002475"
     CDS             119..1711
                     /locus_tag="ACG85N_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0225190.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            1724..2464
                     /locus_tag="ACG85N_002476"
     CDS             1724..2464
                     /locus_tag="ACG85N_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0225191.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            2632..3981
                     /locus_tag="ACG85N_002477"
     CDS             2632..3981
                     /locus_tag="ACG85N_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0225192.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(4109..4699)
                     /locus_tag="ACG85N_002478"
     CDS             complement(4109..4699)
                     /locus_tag="ACG85N_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0225193.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            4846..6198
                     /gene="brnQ"
                     /locus_tag="ACG85N_002479"
     CDS             4846..6198
                     /gene="brnQ"
                     /locus_tag="ACG85N_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0225194.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1926 a         1216 c         1327 g         2029 t
ORIGIN      
        1 ggaattttag cggccttttt atatctataa atcggtcgct ttttattttt tcagataaag
       61 ctttaccgca tttcatctag accgctcgtg tatcatataa tcagaaaggg agaaaaaaat
      121 gaaaaaaaat atggtttcag ttgccgtact atttgctttt gtcttctcac tattcagtgt
      181 gggatctctg gcaaatgcag atgaaaaaac accgagcgat gagtttcgcg taggtatgga
      241 ggctgggtat gcacccttca actggtctca acaaacagat gagaataatg ccttgccaat
      301 tcaaggccaa aatagttacg ccggcggtta tgatgttcaa atcgctaaaa aagtagcaga
      361 tggtttaggg aaaaaattag tcatcgtcca aacgaaatgg gatggtctgg ctcctgccct
      421 tcaatcaggc aagatcgacg cgatcatcgc cggaatgagc cctactgctg aacgacgaaa
      481 agaaattgat tttaccgatc cttattacga atcacaatta gtagtagtag ttcaaaaaga
      541 tagcaaatat gcaaaagcaa aaagtttagc agatctttct ggagcaaaga ttactgctca
      601 gttaaataca ttccactata ctgtgatcga tcagatacca gatgtgcaga aacaacaagc
      661 aatggataat ttctcagcaa tgagaacagc tttagcttct ggaatgatcg atggttatgt
      721 cagcgaacgt ccggaaggtg taacggcaac tagtgtgaac aaagacttgg aaatgcttga
      781 attcagcggt gcagatggct tccagacaaa cccggaagat gttcagattt ctgttggtat
      841 gcgtaaaggc gacccggata tccaaaaagt aaaccagatt ctttctggca tctctaacga
      901 tgaacgaacc aaaattatgg accaagcagt caaagatcag cctgctgcaa ctgatagtga
      961 agaaaaacct ggcatcattt ctgatttcac tacgatttgg aaacaatacg gcaatatgtt
     1021 cttacgaggt gctggactta ccttgttcat cgcattgatc gggacagttg tcggtactac
     1081 acttggatta ctgattggtg tcttccgttc ggtacctgaa tcaggaaatg cactgactcg
     1141 cttcttccaa aaaatcggaa atgcacttct ttctatctat gtcgaagtat tccgcggaac
     1201 accgatgatg gtacaagcaa tggtcatctt ctacggtctg gcactcgctt ttggcatttc
     1261 attgaaccga acgattgccg ctttgtttat tgtctctctc aatactggag cttacatgac
     1321 agagatcgtt cgtgggggga tttttgcagt agatgaaggg cagttcgaag ctgcgcaagc
     1381 cattggtatg acccatggcc aaacgatgag aaaagttgtt attccacaag ttttacggaa
     1441 tatcttgcca gcaacaggaa atgagtttgt tatcaatatc aaagatacag cagttttaag
     1501 tgtaatcggc gtagctgatt tgttcttcca aggaaactct gcttccggtg cgaatttcca
     1561 attcttccaa acatttacga tcgtcgggat catctactta gtcatgacct ttacgatcac
     1621 cagaatcctg cgggcggtag aaaagaaaat cgatggtcct tcagcttata ccaagcttga
     1681 agaaatcgac aattcaaatt tgcaggaata aggaggaaga atcatgaatg aaaaaattat
     1741 tgaaatcaaa catctaagaa aaagcttcgg tgaaaatgaa gtattgaaag atatcagcgt
     1801 aacagtcaat aaaggtgaag ttgtgacgat catcggttct tccggttctg gtaaatctac
     1861 tttcttgcgc tgtatcaatt tactggaaaa accaacaggc ggcgagatca tttataatgg
     1921 agaaaatgta ttagctccta aatataattt acctaaatat cgaacgaatc taggtatggt
     1981 tttccaatcc ttcaacctgt tcaacaatat gaatgtcttg gaaaactgta tgtctggtca
     2041 aacaactgtc ctgaaacgag ataaagaaac tgccaaaaaa gtagcaatcg aaaatctgga
     2101 aaaagttggt atggatcgtt atatcgaagc caaaccagca caattatctg gtggacagaa
     2161 acagcgtgtc gcaattgctc gggcattatc aatgaatcca gatgtattgc tattcgacga
     2221 acctacttcg gcgttagacc ctgaaatggt tggcgaagtg cttaatacga tcaaagactt
     2281 agcacatact ggcttgacaa tgcttatcgt gacgcatgag atggaatttg caaaagatgt
     2341 atctgaccgt gtcattttta tggataaagg tgtgattgca gaagaaggta caccagaaga
     2401 tatctttgtc catccgaaag aagaacgtac aaaagaattc ctttcacgta ttttaaatgc
     2461 ttaggatcaa tagctgattg ggacaaagag ctgcggtgtt taccgcagtt cttttttatg
     2521 aattactcat aaaaggtaaa tttctgaaaa ttttggaatc ttagttgtgt ttttcagaaa
     2581 actcttttaa tatggaagta attaaacttt tttagttagg atgatttaaa aatgaatgaa
     2641 gagcaagaac tgcagcgtgg actgaaaaat agacatgtcc aactgatttc tattggcgga
     2701 gcgattggaa cggggttgtt tttaggttcc ggaaagtcta tccagctagc tggcccttcg
     2761 atattgctcg cctatcttat tactggatgc atctgttttt tcattatgcg tgcgttaggc
     2821 gaacttctgt tatcaaacac caataaccat tcttttctgg attttgttgc agaatatctt
     2881 ggcaaaaaag cggctttcat cactggctgg acgtattggt tctgctgggt atcgattgca
     2941 atggccgatt taacagccat cggaatgtat gtacgctact gggcgcctgg tgttcctcag
     3001 tggcttcctg aactgattgc attaggtctt cttctttgct tgaatatggt ggctgtctca
     3061 ttgtttggtg agttagagtt ttggtttgca ttgatcaaag ttgtagcgat cattgccttt
     3121 atcctcgtag gagcttatat gatcctcacc cactatacga cagatattgg cacagccagt
     3181 atcacgaatc tatggtctca tggcggattc tttccaactg gtgcaaaagg atttatctta
     3241 gctttccaaa tggtaacctt tgcttttgct gggatagaat tagttggatt agtcgctgga
     3301 gaaacagaaa atcctgaaaa agtgttgccg gaagctatca ataatattcc tatcaggatc
     3361 atcttatttt acttaggatc tctttttgtc attatggcaa tctatccatg gaacagcttg
     3421 aatccagata acagtccatt tgtagaagta ttttcggaaa ttgggatcac gattgctgct
     3481 tccttgatca atttagtcgt attaagtgca gcagcttctg cctgcaacag tgcaatatat
     3541 agtacaggac gtatgcttcg ttctttagcg caagaaggca gtgcccctaa aaaattcaaa
     3601 aagttaacaa cacatcatgt gccagggaat gctttgacat tttctactat cgtcattttc
     3661 atttctgtta tcttgaatta cgtgatgcct agtgaagtat tcacactagt aagcagtatt
     3721 gccactactt gtttcttgtt tatctggagt atgcttgtct acacgcacat gaaatatcgt
     3781 aaaagtatcc tcggcaaaaa agctcattca tttaaaatgc cgttgtaccc atttagtaat
     3841 tatctggtat ttctttacat ggcctttgtg tgtgtcgtct tgttcctagg aaaagatacg
     3901 agaatcgctt tattactaac acctgtgtgg ttcttgcttc tattactaat ctatcatatg
     3961 aaatatgaac gtaagaagta agaaagtagt tgtgacagaa gtggtcagct tcgagaaata
     4021 agaaggaatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt atttcttgga
     4081 gttaaaagct tctgtcccag cctcttttct atttccccgg aaacaaccgc tgataatagg
     4141 catctgcttt tttataggaa aaaatccaaa taccagatac aaaaaaacgt ccgaataacc
     4201 cgatcaacgg tacaaaaaag acaccggcaa tcaagcataa gttaatcatc atggagataa
     4261 gacttcgacg gtcctttgac aaaactctca cgacatggga atctggttta tttgctttca
     4321 tgatttgcct catcaagata ccaaaagaaa aactccacat cgtgtatact aagacataag
     4381 ccatctcagg ccaaaaagct tcagggttac gcccgaccca actagtaaca gaaggacata
     4441 aagtcagcca aaagagcaaa aagatattcg accagaccac tcggtaatta attcgttctg
     4501 ccatctgaaa tatacgatga tggttcaccc agatcaccgc aacaaatata aagctgatgg
     4561 tataagctaa taatgtattt cggatgaccc ataaagaatg aaagctagat gtattaggga
     4621 tcttgatctc taatacaaga actctcaaaa caatggctac cactccatca gtaaatgctt
     4681 ctaatctcga ctttgtcacg atgattcctc ctcagatgat tcagcgcttt tattttatca
     4741 cttacgttat taaataaccg tcgttttata taggaaattt tctgaaaatt gttgcatatt
     4801 tttcgaaatt ttgttacttt taaaaaaacc gtaaaggagt tatgtatgac aaaaaaatta
     4861 tcatttaaag actatctttt tataggctcc atgcttttcg gtctattttt tggtgctgga
     4921 aatcttatct ttcccgttca tttgggtcaa gaagcaggcg ctgctacctt ttgggcgaat
     4981 ctaggtttct tggttaccgg tatcggtctt cccttcttag gggtgattgc cattggtgta
     5041 tcgaaaagct caggtgtata tgaattagca aaacggataa atcaaacata tgcgatggtt
     5101 tttaccatct tgctttactt agttatcgga ccgttttttg ctttacctcg ccttgcaacc
     5161 acatctttcg agattggttt agcaccattt atcgatccta gcagacagac gctatatcta
     5221 gcaattttca gtattgtatt ttttgtgctg gcttggtggt tttcaagaaa accgacaaag
     5281 atattagatt atgttggaaa gttcctgaat cctgcatttt tggttttatt aggtgctttg
     5341 cttctccttg cttttttgaa tccactaggt tctgtaaatc aagcgcctat tcagccaaat
     5401 tatcaagagc atgctttttt cacagggttt actcaaggat ataacacatt agatgcgctt
     5461 gctgctttag catttgggat catcattgtc acaacaatcc aaaacatggg tgtaacaaaa
     5521 cctgctgaaa tcgctaaaga cacgatcaaa tctggtgcga tcagcattat attgatggga
     5581 atcatttata ctttgctcgc ttatttaggt gctatgagct taggtagctt tgctctaagt
     5641 gaaaatggcg ggatcacgct ggctcagatt gctgatcatt atttaggtac ttatggcagt
     5701 atcttattag catttatcgt tatattagct tgcttgaaga ccggtattgg tttgattaca
     5761 gcttttgcag aaacattcac agatttgttt ccgcaaagaa attacgcatt ctttgttgca
     5821 cttgctagta tccttccttg tttagcggca aacgtaggct tgacgaatat cattcaattt
     5881 tcgttgcctg ttttgatgtt catctatcca ttagcaatga cactgatttt actagtgtta
     5941 gttggtcctt tgtttaagca gcgtacggct gtttatcgaa tgactactta ttttacattg
     6001 attgcttcga tttttgatgg gttgaacgct tgcccagaaa ctatcaaaca aacacctatt
     6061 gttcaaaatc ttttatatgt tgcggaaagc tacttgccgt ttttcaaatt ggggatgggg
     6121 tggatcgtac cagcagttat cggttttgtt atcggactga tttggagttt cgctaagaaa
     6181 gaagaagtag cagattgata aaagaggttg tgaaagagac gttcagcctt attgccgata
     6241 ggctgtctct tgaacactgt ttattgagag gtcgtgaaaa agccgctcag tttcgagtat
     6301 taagaaatat tttttgaaaa tggcttctca catttttgca taaatttgac ttaataccga
     6361 gaaactggct tttgaacacc gtttattgag cgtctataaa ctgacctgag tattagagaa
     6421 acaaaacggc aaagtagctc catatacttt gccgttttgt tttgaactaa taccgaagag
     6481 aagctgatca aacaacat
//
LOCUS       DAZKLA010000136         6466 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000136 DAZKLA010000000
VERSION     DAZKLA010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.138"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..595)
                     /gene="lepB"
                     /locus_tag="ACG85N_002480"
     CDS             complement(44..595)
                     /gene="lepB"
                     /locus_tag="ACG85N_002480"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0225195.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(747..974)
                     /locus_tag="ACG85N_002481"
     CDS             complement(747..974)
                     /locus_tag="ACG85N_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFQ0225196.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(971..1489)
                     /gene="msrA"
                     /locus_tag="ACG85N_002482"
     CDS             complement(971..1489)
                     /gene="msrA"
                     /locus_tag="ACG85N_002482"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0225197.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(1509..2222)
                     /locus_tag="ACG85N_002483"
     CDS             complement(1509..2222)
                     /locus_tag="ACG85N_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFQ0225198.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(2225..3121)
                     /locus_tag="ACG85N_002484"
     CDS             complement(2225..3121)
                     /locus_tag="ACG85N_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0225199.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(3279..4121)
                     /locus_tag="ACG85N_002485"
     CDS             complement(3279..4121)
                     /locus_tag="ACG85N_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0225200.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            4253..4906
                     /locus_tag="ACG85N_002486"
     CDS             4253..4906
                     /locus_tag="ACG85N_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFQ0225201.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(4969..5496)
                     /locus_tag="ACG85N_002487"
     CDS             complement(4969..5496)
                     /locus_tag="ACG85N_002487"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0225202.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(5516..6463)
                     /locus_tag="ACG85N_002488"
     CDS             complement(5516..6463)
                     /locus_tag="ACG85N_002488"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFQ0225203.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
BASE COUNT         1738 a         1290 c         1137 g         2301 t
ORIGIN      
        1 aataaagcgt gcagtctcaa cagactgcac gctttattgt ttctcactgg atccatttta
       61 tttcatcaag tggataatag acaaatcttg ctttacctag aatatcagtt ttattgatcg
      121 tgccaaacga acgactatct tttgaaactc ttcgattatc tccaagtaca aaataattat
      181 cttccggaag cttggattgt cccgttagct cttgtagtgt aaagtccgtc gtataggatg
      241 catttgcatg atctgatttc aggtttttag ttagataagg ttccttaact actttcccat
      301 tgatcttcaa ttgatcattt tggtagctga cattttctcc tggaagaccg attactcgtt
      361 tgatataaat cgtgccatct gctaattgaa aaacaacaat atcaaatcgt ctgatctccg
      421 aaaatttttc catcacaacc atatcacctt gcttcaacac gttttccatt gaattgcctt
      481 ccactggtac cgggatgaat aaaaatcccc gcaacatgaa cgcaaggaat acagaaacaa
      541 gtaagtattt gaatacaagc cagaagcgat taatatagcg ttgttttttt gtcaatctct
      601 atcaatccat ccctcttttt ttcattcttt ctttattata agaaaaatat caaaaaaatg
      661 cgactagata gctgaaataa aaataattct ttcaattagg ctattggaca aaaaactttt
      721 ttgatcataa agtacaaaac tatctcttat atgtcatgat tattttctaa atatttttcc
      781 catgcttcgt caaagatatc catggacggc aaatagtcga cattcagttc cagataatta
      841 gaaatttctt cgtagctaga gctgtgctta ggaaattgga tgtctttacc ggcttctgta
      901 gcgaaaacag ataaagcatc atgtgtgcca ccgcctcgca gtgtcattaa ataatgaaaa
      961 aagcttcgat tcacccttcg ttcctccaat tttcttcgat aaaggcagcc cggcgtgcat
     1021 ggtttctttt aaaattttca ggatgctttt tataatactc ttgatggtct tcttctgcta
     1081 gataaaatgg ttgggctggt tcgatttttg taacgattgg ctgttcgaaa cgtccacttt
     1141 cagctaactg tttcttactt ttttcagcaa tgatccgctg ttcttctgtc aaataaaaga
     1201 tcaccgggcg ataattgtca ccgcgatcct cgaattgtcc gaaagcgtca gttggatcag
     1261 tttgctgcca gtaaagttct actaattgtt catatgaaat caatgttgga tcatagataa
     1321 tctccactgc ttctgtatgt ccagtcgttt ggctttttac ttgttcataa gtcggatctg
     1381 gtacatgccc tccagtatat cctgacatca ctgtatggat accaggaagc gtgtcgaaag
     1441 gctggatcat acaccagaag cagcctcctg caaatattgc tcgttccatt atgattcccc
     1501 ctctaatact actcatccga tgatttttta ttttcttttg tcttgctttc agtactgctg
     1561 gtgctagttt tgttggtatt tgagttgttt gaagcatcag aggtatcttt tggcaaatat
     1621 agactaaacc gaatgtcatc atttactaag tcgattttat ctgctttaat atacatcccg
     1681 ttttgcattc ggaattgatc caacctaagc atgatagtca gatcatctgt attgacttcg
     1741 atccaatctg gtaatttata gtttctcttg atcatattca tcacatcttt gattggcaaa
     1801 ctcaatgttc cgatagaaag acttctagct tttagttgga catttccgtt ttccatcaca
     1861 tacggatcaa aatataaata gaaatcgata ggaaaaccta gtacttcgaa agtaccattg
     1921 agtagtgctt cattttccaa ataaaattta tattcaatat cactatcttt ttgatactca
     1981 ctaaggaaaa agctgatgat ttgattgact ttttctttat ttgaattgat cgttaagaca
     2041 ggatctcctt gtctttccgt aagagctggt atttctggga gttctggttc ccgattagca
     2101 aaaatacggc tgcctaaaaa tacggtcgaa ccaagtacta ccccaagaag gataaggaag
     2161 gcgaatttcc agtaatttgt tttctttgtt ttgtttgaag atacgtcaga tcttgttttc
     2221 atgtttattg atcatcctcc ttctctaacc attggttttg tgtctcgatc agctgatctc
     2281 tcactgcatt tgccatcaat tgataaccta aattatttgg atggaaatga tcttcttcat
     2341 agagcacatt atttttgatg ccatttagat tttctgtcgt gctagttgtt tgatctgttc
     2401 cttcttctgt gacacctact tcctgattca atccttgata aagcaaatca ttgattggga
     2461 taaagtagca gttcggcatt ttttttgtca ccgtttctgt tcctttgttc caattgttaa
     2521 caattgtctg catgtcagtg atctcaggaa agttcaaata aaatggattg tagatgccta
     2581 aaatgtaaat aggtgcggtt ttattgaggg atcgaaattc ttccaatagt tctgtgacat
     2641 tttcctgata gtttttcagt ggctttttaa aggaattgac cgttaacccg aataaattat
     2701 tttggataac tttcatcaaa tcattaccgc caacagtcag tgtgataaaa tcagctgttt
     2761 tgatattgtt tcgaatttct tcttttgttt ttactcgctt taagatctgg tcacttcgtt
     2821 caccatttac accatagttt tctgtttcaa ccgcggtcaa attataacgg tcttttagat
     2881 catttgcgac aaggggaacg aatcctcctt gcttggtttg atctcctact ccttcagtca
     2941 gcgaatctcc gatagctatg tagtgaagtt tttctttttt atttggatct tctctcacag
     3001 tagttactgg tttcattagt gggccagctt tcggtataga agcagctaaa cctgcataga
     3061 tgatgatcgc aattaataat gggaacaaaa cgaggatcaa atgtcgtttc gttaatttca
     3121 ttttttcacc tcttgtattc gattggttct aatgttatca aaaattttat caaaaaggta
     3181 gtctttatga aaaaagaaaa caattatttt cctaatttta taaagaaaaa gagaataagc
     3241 tgcaagcctt tcaggctgag ggcttatcct cttttcgact aatctacata atacatgatt
     3301 gcaaacgcgt ctttaccagt atgcgtagcg ataaccggat tcgtatgcag tacagggata
     3361 tgcatgtccg ggaataattc ttgcaaaccc tctttgaatt cttgcgccaa ttcttttgca
     3421 tccgcatgtg aaataccgat ttgtcgcaca tttggcaatt ctttcagttc atttttcaaa
     3481 ttattaaacc atttatcgaa cgttttcttt cctcgcccct tagttacagg gattaattca
     3541 gaatgggcga aattcatcac cacgcgtatg ttcaaaacac tagacagcaa accagttgtt
     3601 cggctgattc ttccaccttt taccaagtta tctagtgtgg atattccgat gtataatttt
     3661 gtattgtctt gtactttttt gacttcttca aggattttct ctactgtttt accttctttt
     3721 gctaattcag ctgctcgaat tacctgaaaa gataatcctt ggtcagtggt atcactgtcg
     3781 ataaccgtta ctttcgtttt tgttagatta ctggcttgtc ttgctgcttc tactgttcca
     3841 ctcaagcctt ttgtcatatg gatcgaaatc acttcactgc cgtcttctcc tagctcatca
     3901 tacatcgaga tgaattctcc aattggtggt tggcttgttt tgggcagatt ttttgctttc
     3961 gccatcattt ccataaatct ttcgccgtct agttggtcat catcgggata tactgttccg
     4021 tcgatcatga tggatagcgg catgacacga atgcctaaag catcgcgtac atttttttcc
     4081 atcgtacatg aagaatctgt tacgattttt acatttgtca ttattttctc tctctctttc
     4141 tttaacaaga tgccttattc atgttaaact acatgtaaga caatttctat ttttagtata
     4201 acagacaaat aatgattttt cattaattac acctagtaaa ggagccgttt tcatggaaaa
     4261 aacgaaattt aatcgaacgt atgaaatatt aaatgaagtg tttaatgcag tcacccatgg
     4321 aattgggact ggattgagta tcgcgggact agtggttctt ttagtaaaag gtgcccactt
     4381 gcattcctcc ctccatattg tctcttatgc tatctatgga tcgatgatga ttcttttatt
     4441 tttaacgtct actttgtttc attcattgat ttttacaaaa gcaaaaaagg tgttccaagt
     4501 attcgatcat agttcgattt ttcttttgat tgcaggaagt tatactcctt tttgtttgct
     4561 gagtatccga ggatggctag gttggacttt atttgtatta atctggttgt ttgcgattct
     4621 tggcattata tataaagcgt tgacgttgca taaacgggag agagtatcaa aagtatctac
     4681 gatcatctat atttttatgg ggtggctctg tgtcgtggct gctgttcctc tttatcactc
     4741 tcttggaatg actggtgtta ttttgatggt agcaggcggg ctttcttaca cgataggcgc
     4801 attcttttat agcttgaatt ctatccgcta tatgcatgtt gtttggcatt tgtttgttat
     4861 gttaggtgca ggttttatgt acttctctgt tttattcttt acttaataaa aaaaataaag
     4921 ctatggatgg gacagctcta aataacagct agtctatcca tagctttttt atatcgtcaa
     4981 ttcgcctgtc ccgcttttta caggattctc attacgctga tacgtttcaa attgatgcgg
     5041 ataacgattt ttttcatcca tttctccttg actagtattg atcagtgacc aatcagacca
     5101 gtcaacaggt ggaaaataag catctccatc gaatacatga tggatgactg tacggtataa
     5161 tatcgtacaa aatggcagaa attcttgata aacagctgac cctccagcga tgaaaaagat
     5221 accgtctgtt tctttttcca gagcaagcac gtcttccaca ctatgcatga catgcacgcc
     5281 ttctgcttta taggaagaat ctctcgtcaa aacaatcgtc tgacgattcg gcaacggacg
     5341 gctacccatc ccttcgaatg tttttcgtcc catcactagt gtatttgctt ctgtcatttg
     5401 tttgaagaat ttcagatcat ttggcaaacg ccaaggaaga cggtcttctt ttccaatcaa
     5461 tccgttttca tcctgtgccc aaattgcagc taacattttc tcatcctttc tgattctaaa
     5521 cagcgatagg agctttgatc gccggatgtg ggtcatatcc ttctaattgg atatcttcca
     5581 tctcaaaatc aaatactgac tgcttttcct gattcaaaac taaagtcgga aatgcgcgta
     5641 tttctcggct caattgttct ttcatttgtt cgatatgatt gatataaaga tgagcatctc
     5701 ctaatgtgtg aacaaattcg ccaaccgcta aacctgtttc atgtgcaatt aggtgagtca
     5761 gcaacgcata acttgcaatg ttgaacggaa cgcctagaaa cacatcccca ctgcgttgat
     5821 acaattggca gcttagccgt ccctcttgaa cataaaattg aaacatcgta tgacaaggag
     5881 gcaaagccat gctaggaaca tcttccggat tccatgcaga aacgatcaat cttctagaat
     5941 caggagtctt tttgattgct tcgatcactt gtttgatttg atcgataaag ccaccgtctt
     6001 ttgtttccca gtgacgccat tgatagccgt agatgtgtcc aagatcgccg tatgtttcag
     6061 caaaggaatc atcggttagg atcttctcgc aaaaaagctt gctctgcttt ttatattctt
     6121 cattgaattc aggatctgtc aaacatctat ggccaaaatc agtcatatct ggtccattat
     6181 agtctggact tttgatgtag cgttcaaatg cccattcatc ccaaatatgg tttttatttt
     6241 gtaatagata gcggatattc gtatctcctt ttaaaaacca aagcaattcg cttttgatca
     6301 atccaaaagg tactcgtttc gttgttaaca aaggaaaccc tttttgcaga tcaaaccgca
     6361 tttggtatcc gaataagctt aatgtgcctg tgcctgttct gtcttcttta cgatgccctt
     6421 cttccaacaa ttttcttcct aagtcaagat acgcttgttc catcct
//
LOCUS       DAZKLA010000137         6329 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000137 DAZKLA010000000
VERSION     DAZKLA010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.139"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..276)
                     /locus_tag="ACG85N_002489"
     CDS             complement(<1..276)
                     /locus_tag="ACG85N_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225204.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLK"
     gene            complement(484..1410)
                     /gene="glsA"
                     /locus_tag="ACG85N_002490"
     CDS             complement(484..1410)
                     /gene="glsA"
                     /locus_tag="ACG85N_002490"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFQ0225205.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1433..2860)
                     /locus_tag="ACG85N_002491"
     CDS             complement(1433..2860)
                     /locus_tag="ACG85N_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0225206.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2891..4105)
                     /locus_tag="ACG85N_002492"
     CDS             complement(2891..4105)
                     /locus_tag="ACG85N_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFQ0225207.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(4191..4418)
                     /locus_tag="ACG85N_002493"
     CDS             complement(4191..4418)
                     /locus_tag="ACG85N_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225208.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(4446..4688)
                     /locus_tag="ACG85N_002494"
     CDS             complement(4446..4688)
                     /locus_tag="ACG85N_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0225209.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(4720..5277)
                     /locus_tag="ACG85N_002495"
     CDS             complement(4720..5277)
                     /locus_tag="ACG85N_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0225210.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(5290..5478)
                     /locus_tag="ACG85N_002496"
     CDS             complement(5290..5478)
                     /locus_tag="ACG85N_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0225211.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(5491..6057)
                     /gene="amaP"
                     /locus_tag="ACG85N_002497"
     CDS             complement(5491..6057)
                     /gene="amaP"
                     /locus_tag="ACG85N_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0225212.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         1908 a         1325 c         1078 g         2018 t
ORIGIN      
        1 ctttaaaaaa taagattgac cgtacagaaa ctgtgtgagc tcagaaagag caataccatg
       61 tttgaatacg agtttatact tatgcttctc tcttacttgt tcttttatac aatattctag
      121 acactcggct agactttcta gatcgtgaag gatcgttggt gtagtcaccc ttagagaaat
      181 agctgtttgt tcgatgtcta tttcttggaa ttcaaataat tgttcacaca gatacacttt
      241 tctaaatata tttcgctcga tgtaattttc cagcattcct gatacacgcc tccagttttt
      301 cttatgattt tatttaatca tatctgtaaa tcgtattttt gtttcctttt tatttagaaa
      361 agccctttca aaaaccaata aaaaaagaaa ctgggacaaa agtcaaacaa gctcacaatt
      421 tcagcctcat tgtaggtagc tttgagccgt ttggactttt accccagcct ctttcaagaa
      481 tggttattca aagatgtcag cgtctagttc tttgactaca tctttcaaca attggatacc
      541 agctgcgctg ttaccaaatg gatctagtgc cgggctgaag acacctatgc cgtaacgatt
      601 tggcacagct gccatcaacc cgccgcctac accgcttttt gcaggtacac caacatggac
      661 ggaaaactct cctgattcat catacaaacc agcagttgtc ataattgatt tgacgattgt
      721 agcactttct tcagtaatga tttgtttgcc attccaagga gcaattcctt tatttgccag
      781 cacagcagct aattttgcta tgcctgtagc ggtgacattg agggagcatt gtctgaaata
      841 agcatcgagt atctccggta cgtctccttc gatcatttga ttacctttca tatagtaagc
      901 aagagagcga ttgatatcac ctgttcttga ttcagaatga tagatttctt cattcaatgt
      961 gatattagga tcatcacaga tctctttcat aaatgacaag attcgctcaa atttttcatc
     1021 tgcatcttta ccggcaatca acgaggttgt tgcaatcgct cctgcattaa caaaaggatt
     1081 cattggatgt ttgcgatgat tgatttccat attcatgata gaattgaagg caaaaccggt
     1141 tggttccgtg ttgattgttt gaaagacttt ttctattccg ttgtctttga ttgctaataa
     1201 caatacggga acttttgaca tgctttcgat ggcaaagcgc acatcggcgt ctcctccctc
     1261 aatcagttgt tttgtatcca aatcataaat cgccatacct aattgtttgg gatccacttc
     1321 agctaatgct ggaatgtaat ccgccacttg tcctgtttga taatagtgga aattttttcc
     1381 aatcaattgt tctaattctt ttacttccat ttgaataacc agctttcatt ttttattttt
     1441 ctgcatgctg gaatttatga cggcatgcgt agatcgtaaa tggaataact aagacgataa
     1501 gataagaaac aatcagaatc gtttcataaa tcccatgctg tccagctgcg atactagcag
     1561 gtggaacaaa tgagatacaa aaagcaaaaa gtgaagtcaa aagacctaaa caagcaacga
     1621 ttacttttac aacttttccg cctggaacct gataggctct ttcaagactg tcatctttaa
     1681 aaaccagctt caaatagccc gcgaaaaaca taaagtacaa catcaggtag attacaacag
     1741 tcaatgacat cgctgcaaga aatgaaagat tattcccccc gccgcttaat gtaaagacgg
     1801 ctgcccagat tgttgcaacg atcccatttg cgatcatcaa atgagcagga acaccgtgtg
     1861 tgttttcttt tgcaaaatat tcaggaagta atccttcttc tgctgctacg tgaagtgctt
     1921 ttgaaggaga ggttacccaa gaagcaattt ctccgatgac acctaaacaa acaagaatcg
     1981 caagaatttt cgcaatccat gcaagatttg gatcaatgtg tcttaatagg aattctaacg
     2041 attgaatcac ccctgtatta agagacaaac catgttgagg tactgtagta gcgacggtca
     2101 atccgccaaa agtatctaaa atgattgcca gaataaccaa tacaaacatt gctaatggat
     2161 aatccttttt agggttttcc atttcatttg catgactggc agatgtttct acgcccatat
     2221 atgctaaaat aaaggaaaca aaaacaacca gcgtaggcaa tttcgaaaaa tcaggaatca
     2281 atgttttcat agacagtatc gtttgtagtg gagcgcctga agcgacataa tggatcccta
     2341 agccaaacaa gactaatgct ggcaacaaga taccagctac aaaaccgatc cgagcaatca
     2401 atgaagtgta tttggttcct tttaactgtg agagtaaaat agcccagaaa ataatcaata
     2461 ggattgccaa tttcagatat ttattgtttt gtatagcacc aatacccgtt gcataggata
     2521 atgcaccagt taagaaatac agcatggtca agtagccaac tgttatctca aaccattgga
     2581 aaaagattgc ggcaaaaccg aatcgttttc ccaatgtgcg actgacccaa gtatagacac
     2641 cgcctttttc ccatcctttc accgttgcca tctctgctgc gcacaatgcc acagggataa
     2701 accatagcaa accgcctaat aacaagaaaa atactaagct gaagcctgag gtcgcgaatg
     2761 tgggatattc atataatgaa acgaccattg agatggtcat agccaaaaag ccaaagaacg
     2821 ttaatttttt tgcaggaatt ttcgttttgc tttgttgcat gaatcttcct cttttctatt
     2881 tgaatttgtt tcaattgagt ttgttgccaa agaagaaaaa gtccaatcct tcttcttgtt
     2941 cttcttttgt agtagtgatc gttgtaaagt gcatcgccga ttttaaaatc actgttgtca
     3001 gcacataggc atacacggca gtaatgatga caccaatcac ttgaatccct aagaagtacc
     3061 agctgcttgg attcgttagt aaatggctgt tagatgcaaa gatccctatc agaatcgtac
     3121 ctgtgaatcc gcccatgcca tgaacacccc acacatcaag tgcatcatcc cattgtttct
     3181 ttttacgaaa atcaacagca gcatgacaga cgatacccgc aatgatccca atcagcatgg
     3241 cactttttgc ttctacataa ccggcacaag gcgtaatggc tgccaatcca gcaacggatc
     3301 ctgttagtac gtcgacaaaa tcaaagccat ttccttttac ttttgcaatg atcagccaag
     3361 taatcatcgc aaatgcaaga gcgatgaatg ttgaaccaaa tgcattgact gcttgttcat
     3421 ctgctcgaag tgcaccaccg gaattaaaac cgaaccagcc aaaccaaagc agtcctgtac
     3481 cgatagcagc tgccataaga ctgctatgct gacttctttc tcctaaagga atctttcttt
     3541 gaccaagtgt gaggatacat gctaaagagc cgaatccagc tgtcgtgtgg atgactgctc
     3601 caccagcgaa atctctaaat cccatctgat ccaaaaatcc gccaccccat atccagtggc
     3661 aaacagggaa ataaatcagc agattccaaa aaataacaag tataatatag ccttttaaat
     3721 tcaatctccc tgcaaaagct cctgtcatca aaggaacggt aatcacgata aacatcagtt
     3781 ggtaaagaaa gaacattaaa aatggaatcg ttgcaccatg aaacatgttg gggaagcttg
     3841 taacatgtct catcaaaaag aaatctgccg ggtttccaat aacaccgccg atatcccgtc
     3901 caaaggccaa tccgaatcca ccaaataccc acataatcgt tgttatccca acggagagaa
     3961 aagattgaga catgatcgtt agtatattat tcttccgtgc taatccgccg taaaaaaaag
     4021 ctagaccagg agtcatcagg caaaccattg ctgtacataa aatcatgaat gctgtatctc
     4081 ctgtattgat aggtaatgta ttcatttttt tcgcctcctt tctacgatta cgagtataaa
     4141 agaaggtgta gttgtttttt tcttctgttt ttttgaatta atacttgcat ttataaaaga
     4201 cagacaatta ataaaagctt gatgtgttgt gctctttttc gtttttcaca caaataaaga
     4261 caaaaaataa acggtcttct ttttatagaa aaccgtttat tttcgtcttt ttttattttt
     4321 aaagatattt tttcataagg aataaagaat acttcatata aaatatataa aaaagaaccg
     4381 cagcgagtgt cgctacggtt ctcaaatttg ttcttcattt caacataagt ttttcttcaa
     4441 tttgcttata atttacgtgc aaagaatgaa acgactaata ctaaaatgat tgcaccgatc
     4501 aatgatggaa tgatagccat tcctgcaatt gttggtcccc aagttcctag taaagcttgt
     4561 ccaattgctg aacctactaa accagcaatg atgtttgcga tgatccccat tgatgatcct
     4621 ttattcgtga ttgctcctgc aattgctcca atgataccgc cgacgattaa tgcccaaata
     4681 aatcccattt taaattcctc ctaactgact tcttacttct tattgtacac gtggttcgtt
     4741 attttctttg actttttgtg cacctttgcc tacagcagat tttgctttat ctgtttggcg
     4801 tgaagcaaat tgtcctgtgg attttgctgc ttctgttact ttgtcttgta ctgtttcttg
     4861 atctttttgg tattcttctt gtgttttgat gtctgcaaca ttcacattca cttcgataac
     4921 atctaaatgt gtcatattgt ttacttcagt actgatcaat tctttgattt ggttgtaaat
     4981 atcttcgatg tctttaccgt attctacgat cacatctaga tccacagcta cttgtttctt
     5041 acctacttct gtattgatac ctgctgttac gttatctgtg ttgactaatt tttcagccat
     5101 atttgagaaa aatccgccat ctaatgtcaa tagaccatct acatctttca atgcataacc
     5161 gatgattttt tggatgactt tgtcttcata agtcaattca ccattcatca tgcggttttc
     5221 tcctttttca tttgccaaaa ctgatccatt tgttgtattc atgtttttgt tttccatctt
     5281 tgtttcctcc tatgatttaa aaaattgatc aactattcct gactgtttca aataaaaacc
     5341 gattcctgcg ccaattgctg tcatgattat gatcaacaat gtcttgaaaa atccaatcgt
     5401 taaccataaa acagctagca gtagtccaag acccccaaag ataataggga gcttgtatgc
     5461 tgaaaatagt tctttcatta tttggccctc ctattccact cgagagtgct tgtagtctct
     5521 tgtatcttct tgttcttcat aaccgctgta tgtgactgcc actttgaccg tttcttttgt
     5581 tccgagtact ttagttactt cttcttcgat atcttgcatc aatacacctg ttttccctac
     5641 taatgaagaa gttcgtttca agtctccgtt gacatggata tggattcgat ttttcgttga
     5701 acgtatccgt actttaggtg aatgaatgaa ctcttcttcg tgtaaatggg aacgtaccaa
     5761 cccttcgatc gcttttttgt cgattgttag tttgccatct tcccgtttca taacgaaagt
     5821 tcctcttgac tttggataga aaagaacgac tagcaagatg ataatagcaa gaacggcaaa
     5881 tactacagct gcccagaaaa tatattgttg catgtagtaa ttcgttaaag taaagaagcg
     5941 aaatcgttcc aattgttcag gcataatata cggcgcattg atcaacgcaa tcgctagaaa
     6001 tactgaaagt agcagtaaac tcatgataac accgattatt tttaacccct tattcatttt
     6061 ccacctccta taaacttatt tttaccctgt aaataactct accgacaatc acagtataga
     6121 tagcgaacag ttagcatagc atcagtaaat aaagcgctac caaacattgc tggatcataa
     6181 cggcgtttta ttgccagtct gatactgaaa taacaattgc ttacttattc tctatttact
     6241 tttgattgct ggctatactt aaaggatatt attcaagatt aaaaattgca aagattatgc
     6301 tcagttatta ttacaagatt ctatttatt
//
LOCUS       DAZKLA010000138         6324 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000138 DAZKLA010000000
VERSION     DAZKLA010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.140"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG85N_002498"
     CDS             complement(99..1229)
                     /locus_tag="ACG85N_002498"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFQ0225213.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACG85N_002499"
     CDS             complement(1878..3080)
                     /locus_tag="ACG85N_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0225214.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACG85N_002500"
     CDS             complement(3140..3343)
                     /locus_tag="ACG85N_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0225215.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACG85N_002501"
     CDS             complement(3485..4552)
                     /locus_tag="ACG85N_002501"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0225216.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACG85N_002502"
     CDS             complement(4610..4717)
                     /locus_tag="ACG85N_002502"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0225217.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACG85N_002503"
     CDS             4880..5692
                     /locus_tag="ACG85N_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0225218.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACG85N_002504"
     CDS             complement(6066..6257)
                     /locus_tag="ACG85N_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225219.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1881 a         1299 c          997 g         2147 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaa
//
LOCUS       DAZKLA010000139         6272 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000139 DAZKLA010000000
VERSION     DAZKLA010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.141"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..490)
                     /locus_tag="ACG85N_002505"
     CDS             complement(164..490)
                     /locus_tag="ACG85N_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225220.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(536..898)
                     /locus_tag="ACG85N_002506"
     CDS             complement(536..898)
                     /locus_tag="ACG85N_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFQ0225221.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(935..1149)
                     /locus_tag="ACG85N_002507"
                     /pseudo
     CDS             complement(935..1149)
                     /locus_tag="ACG85N_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1277..1612
                     /locus_tag="ACG85N_002508"
     CDS             1277..1612
                     /locus_tag="ACG85N_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0225222.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(1688..3685)
                     /locus_tag="ACG85N_002509"
     CDS             complement(1688..3685)
                     /locus_tag="ACG85N_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225223.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(3783..4058)
                     /locus_tag="ACG85N_002510"
     CDS             complement(3783..4058)
                     /locus_tag="ACG85N_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0225224.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            4290..5024
                     /locus_tag="ACG85N_002511"
     CDS             4290..5024
                     /locus_tag="ACG85N_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0225225.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            5063..6205
                     /locus_tag="ACG85N_002512"
     CDS             5063..6205
                     /locus_tag="ACG85N_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0225226.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         2138 a         1146 c          900 g         2088 t
ORIGIN      
        1 ttatattgtt ataacagtga ataacataaa aattcccgac aatcattgta atattttttt
       61 gtcacctgta ttagtattta atttatagga aactataaaa actccctagt gaatttacac
      121 ctttgggagt tcctgtttgg acggtatgca taatacattt aaatcaattt aacattaaca
      181 cctcagcata tggtatctcc acatatatac ggtctttctt tggatagtac tcaatattta
      241 tgttgatagc acttccattg tctaatatag ctttagcccg gtatgtgtta tctccatttt
      301 catgagtaat cgtcacatca tcacatgatc gatttaagtt gtactgttct tgtaaaatct
      361 gtgttaccaa tggtttcgat gacttctcta atccagatgt acctgtcatt tttgaaaaaa
      421 tgatacctat aatagccaag acaacgataa ctataatgat aaacttcttg ttcatattta
      481 ctttattcaa ctttttatct cctatctcca aaataatttg ctcttatccc tcttatcagt
      541 acattgacat ccctaaaagt acaccaaaga ttgctccagc tacccctgct atcatcaaaa
      601 tcgtacccat ttgttcatct ttatctcgat ataagtagcc cataataaca gcaacagcac
      661 caaatacaac aggaataaat gctaacgcta ctaccataga aatccaacca ataatcaaat
      721 acagcttccc tgtctcttgt ccatgtacga tttgttcctt catttcatta attgtctgct
      781 cagtactttt ctttactgcg tctgctccag ttgtctcatc tgtttcttgt agtgctccgc
      841 aaaactggca atacttactt tcatctgtta tttctttgcc acattttcta caatacatgt
      901 cttctcctcc taaaaacatt caattgttta ttagttaact gtttacttta ttaaatacat
      961 cagaaaacag ctcattcatt ggtacactta atatctcaga aagcatcttc atttgatagg
     1021 gtttaggaat cgttctccct gtttgccacg aacttacaca ttgctgtgta acattcattt
     1081 ttttgctaac tccgtttgag aaagtccttt ttcttttctg tatcggctga gattacttac
     1141 ttttttcatg attctcccct cctgttttat tatatacaac aatatgatgt atttcaactc
     1201 ataaaatata aaaaaacttc aaataccatt acaaatacaa caacttgttg tactatactg
     1261 agatgggggt gtattaatga aacagaaaca atatgcaaag gtcattagag aaaagcgaaa
     1321 agctctcggg tggacacaaa aagaattagc tgaaaaaatc ttctctacac aacaggcagt
     1381 agcaagatgg gaaaattctg taacagagcc taacctcgat agcttaaccg ctctttctag
     1441 agccctagga acccctgtaa gccattttct agacaatgtt gtcgtagatt atgaggaaga
     1501 gtttttggct ctctatcgtt ctcttagcac tgaagatgca gttcgaacaa ttgactatat
     1561 gaagctatta aaaaggcaag aaaatgaacg caatcagcta ttgaaagatt gaaaaataaa
     1621 taacccaccg aactttaacg gtgggtgtat tgagatgaaa taagattaag agctatggat
     1681 aacctgtcta gtttaataca attgggtctt ttctcttcct tactctccgt ccatcatctc
     1741 ttgttacact tgtaaattga taaagaggaa ctaattcaac tcctgataat atcaattctt
     1801 ttattacttc atctatccct gctccttttg ctaggtagac taaaaaacta ctacttgctt
     1861 tcacattgta tagtctctta atatccttag aagctcgttc aagagtgaat ccttgtttaa
     1921 caaagtagcc tctgttttct tttaaagcat tttccacgct gttattccca acattacgta
     1981 gacgttcaca aacagtgtta gtaagttctt tctctgtcat tagatatgtt ccttgccacg
     2041 ataagccctg taaaacttca tgtgcttcat ctaaaatcaa ttgtttttcc ttgctaagtt
     2101 ctgatcggga attttgagga aatactcttt tagcctcttt ttcgttaaaa catatcttaa
     2161 cccattcata agaaaaatat tgatatgtaa aatgaaattt ttccagaagt ggaaggatat
     2221 cttcattcaa tttttgaatc acagaagttg cattaaaaac gtctaatcga tagacattgc
     2281 ttctcttatg attaaagttt cctaacgctt tctcttcctt taacctattt aggtattcga
     2341 gcatcttcaa aggaacatct tcatcatcta atttttttat caatcctata aaagtcataa
     2401 gtgatagtaa cttattacat ttatctatgt cataagtttt accatagtaa aaactcattt
     2461 ttttagctaa ttgttccccc gacaaaatac aagtcacact ttccttacta tcatcaaaaa
     2521 aattaacatc agaaagcaag acatcataga cacgtcgcat ttcctctaga taattcctta
     2581 ttgtcctgaa aagtttgtct ttcattgatt caagagtgtc aaaaagtact tttgattcct
     2641 ctttcaattt tcgaaaatgt gtaggataca agtctaggtt ctctaatagc caattaaatg
     2701 tttctacccg actgtctttc tttaaaatta gcatcacaac tccaataaaa ttttcactga
     2761 tatcacaatt actggaatga caataaaaaa tggaatgccc agttttactg gtaaaaatac
     2821 tagcagaggg attatgatcg ttgtgaaaca gacatcttaa gtttttactt gacttaagta
     2881 atttattcat atcaatggtt aacaatcttt cataaatctc attaaaagac atgtcggagg
     2941 aatcaaacag gcagtcttta aaccactgtt tcatttcttc tttgtcgtca ttttttatca
     3001 acttaacaaa atcggtttca ttgactagtt catactctct ccctgaatca ctttgacaat
     3061 ttttagaaca tgtcatggta aaatcaatcg gaagctcagt aatatctaac atgttgtctt
     3121 cattaattat tatcactgca tctgacttat atcctccaaa aaacattcta ttcgaagcat
     3181 ttacattgtc atcacacccg gggatgttct ttttcaagta ttcgtaaaca gcaaccattt
     3241 taccatagtc agtaattgct tcttttaata gaaagacaat cttgtatttt tctagatttt
     3301 cagagtaccg aatacttttt tgtatcgcaa acgcagaatt atgaactaaa ttgatagata
     3361 aaaccttttc tatgcttaag tatctactat catctttact caaatctact accttcccct
     3421 tctcatcttt aacagtgtta tcgacatcca gaataattac tctggtttcc acaaaactct
     3481 ccttgtcatt gcctttattc tttgtcttgc ttggaataaa tgattgtgat gaatttagta
     3541 tctctacaaa ctcatctctt gtaacttctc tatagttatc taaacaccta tctcttacat
     3601 cagttttttc tttttttgta actggcttct ctaataattt ttctttatct acataaattg
     3661 gaatattaat cattgatgtt tccatttgtg tttcctcctt ataaaattct gttaaagact
     3721 aaaacataca gataataaaa acaaactctt tattactata tgttttagtc ttatttgttt
     3781 actcaacctt tataccgttc catgaaacta tcaatatcag attttttaaa tctaatgacc
     3841 gtatcaattt ttataacagg taacccgtat tgagaaacat attttctaat cgttcctact
     3901 gatacaccta gatattcact tgcctgctga tagtccatat actctagagt gactccagaa
     3961 ataatgtctt ctagcatagg ctttagcact gtaattatct ctttcacttg ttgttcaact
     4021 tgtatagaaa caatgctatc aatgatttgt agagacatat ttccccctcc tttcgtgatt
     4081 gcttgaacaa attttctaag aattatcttc gtattttttg ctcaaaataa aagtattttg
     4141 ttattaaccg attaatcaaa caaaaaatga ttaccagttc atcgacatac acataatacc
     4201 tcgataaaaa agaggtgtca atatctttgt aaaatttatt tacaacaccc ctttttcgag
     4261 gtatactaat tttaggaggt gtggtcaata tgataaaacg tccaccaata aattatttag
     4321 aacgaaaaaa gatactcgga acaaaaataa aagctataag aaagagtaaa aaattaacac
     4381 aaccagcatt tggtcttatg attaacaacg gtcaattaat tgacaaaaaa actatctacg
     4441 aatgggagaa gggaacctat ttaccaatac cagagcgatt atcccgaatt gctgatttgg
     4501 gaaatatgag tatagaggag ttagtttgtg gcaatgtaga agaatatatt ttgggaatta
     4561 ttctttatag agatagcatt gtactagatg gtataacatt tccagataaa aacctgttcc
     4621 agcatcttcg tcaacagttc ccaccagtac actctaattt agatacatgg ctggatagat
     4681 attccaaatt agaaccagag atgcaagaat ttattgctaa caagacttgt aataaagtta
     4741 aaaatgaaaa aattagtctt ttcaatatct taaaaataga agaattattc atcaatgcta
     4801 ttgtagaaga atttgacaat aacattcttt tcctcactag cagtatagaa gaactactag
     4861 aaagaatggt agatgaatgg ctacctattc aattgaaaga catgtcttat cccgaagaag
     4921 ctgtgagaga aattactgat aatataaaca agctagaaca aacgatttca tcaattggga
     4981 aaaaatacac taagaaaaaa atgaagggag gtgataccat ctagtggtaa gtactccttg
     5041 taaactaaga ggagatgatt tcatggcaaa aatatcaaag tacaaaaaga aaaacggtca
     5101 agtggcgtgg atgtataaag gacacattgc caccgatcca cgaactggtg aaaaaataaa
     5161 tactacgaga agaggtttta gtagcaaaca agaagctcaa aaagattatg acgagtacag
     5221 acatcaaata ttatacggtg ttaaaaagaa agcacctgat atgacatttg aagacctgta
     5281 taatgaatgg ataactcatc agcgtaccag tgttaaagct tcaacaatcg caatctcagt
     5341 aagatacgca aataatcaaa tacttccagc atttggtaag cttaaactat cgaatatctc
     5401 tgtcccatat tgtcaaaaag tggtagatga atggcacagt aaatatgaaa gttacgatta
     5461 tatgagaaaa cagactgcac aaattttacg ctacggggtt gcaatgcaat atattgacaa
     5521 taatcctatg gaaaaaacac ttcttccacg aaaaaaagag tatgagaaaa atcgtaaatt
     5581 ttactcaaaa gaggagctta ataatttact ggatgctttt aaagattttg gtaacatgaa
     5641 acaatacgcc ttcttcagat tattggcata cacaggtatg agaaagagtg aagtactcgc
     5701 tttacagtgg aaagacatag acacgtttaa taaagaacta catgtcaata aaactcttgc
     5761 tgtagatgaa tttggtaaag ttataattca atctccgaaa acaagagctt ctagacgagt
     5821 tatctcttta gatactgaga ctctttctat cctcaacaac tggaaattac agcaaaaaga
     5881 agagtattta aaacttggat acaatactag ctctaaagag caacatgttt ttacaacagt
     5941 taaaaacact ctatacattc caaacactgt taatgattgg ttaagatata ttttgaaaaa
     6001 atataacttg cctcgtatta cacctcatgg gtttagacat acacatgcta gtttattatt
     6061 agaagcagga gaatctgtca aggtagttca acaacgatta gggcatgaaa atagtaaagt
     6121 gacattagac atctatgcac atattacaaa taatgcacct aagaaaacag gtcaagactt
     6181 tgctgatatg atggctcatc agtaaaagag ctggtcaaaa tctggtcatt tctagtatac
     6241 aggcaaaaca aaaaagctag aacccttata ta
//
LOCUS       DAZKLA010000140         6173 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000140 DAZKLA010000000
VERSION     DAZKLA010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.142"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACG85N_002513"
     CDS             725..811
                     /locus_tag="ACG85N_002513"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0225227.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACG85N_002514"
     CDS             complement(932..1621)
                     /locus_tag="ACG85N_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225228.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="ACG85N_002515"
     CDS             complement(1628..1906)
                     /locus_tag="ACG85N_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225229.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="ACG85N_002516"
     CDS             complement(1866..2780)
                     /locus_tag="ACG85N_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFQ0225230.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACG85N_002517"
     CDS             complement(2762..3142)
                     /locus_tag="ACG85N_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFQ0225231.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACG85N_002518"
     CDS             complement(3592..3879)
                     /locus_tag="ACG85N_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0225232.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACG85N_002519"
     CDS             complement(3899..4123)
                     /locus_tag="ACG85N_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0225233.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACG85N_002520"
     CDS             complement(4578..4748)
                     /locus_tag="ACG85N_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225234.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACG85N_002521"
     CDS             complement(4763..5296)
                     /locus_tag="ACG85N_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0225235.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACG85N_002522"
     CDS             complement(5317..>6173)
                     /locus_tag="ACG85N_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0225236.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAZKLA010000141         6033 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000141 DAZKLA010000000
VERSION     DAZKLA010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.143"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            81..515
                     /locus_tag="ACG85N_002523"
     CDS             81..515
                     /locus_tag="ACG85N_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0225237.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(593..1126)
                     /locus_tag="ACG85N_002524"
     CDS             complement(593..1126)
                     /locus_tag="ACG85N_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFQ0225238.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1220..2398)
                     /gene="mutY"
                     /locus_tag="ACG85N_002525"
     CDS             complement(1220..2398)
                     /gene="mutY"
                     /locus_tag="ACG85N_002525"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFQ0225239.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2391..3188)
                     /gene="recX"
                     /locus_tag="ACG85N_002526"
     CDS             complement(2391..3188)
                     /gene="recX"
                     /locus_tag="ACG85N_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFQ0225240.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3295..4677
                     /gene="rlmD"
                     /locus_tag="ACG85N_002527"
     CDS             3295..4677
                     /gene="rlmD"
                     /locus_tag="ACG85N_002527"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0225241.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4851..5429)
                     /locus_tag="ACG85N_002528"
     CDS             complement(4851..5429)
                     /locus_tag="ACG85N_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFQ0225242.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5751..>6033
                     /locus_tag="ACG85N_002529"
     CDS             5751..>6033
                     /locus_tag="ACG85N_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFQ0225243.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWS"
BASE COUNT         1788 a         1185 c         1081 g         1979 t
ORIGIN      
        1 ttttttattt ttgaaaactt gccatacttt ttatttgttt tattttccag tatactatac
       61 gaaaggaagg tggctagacc atgaaagttg tacaaacaaa agatacaatg agcaatattt
      121 acttagatgc tgtacggatc aggcatcaag tttttgtagt cgaacagggt gtgcctttgt
      181 ctcgagaaat agacaaagat gaagcccatt gtatccactt cgtcctgtac tcagataaaa
      241 aagaaccgca aggaactgta cgtcttttgc ctttagaaaa cggaaaaatg aaattacagc
      301 ggatggcgat tctttctgaa tacagacacc aagggttagg aaaaatattg attgaagaag
      361 cagaaaactt tgctaagaat caaggctata atacgatttt attaggggca cagtccacag
      421 ctgaaacttt ttacgaaaaa ctaggatata cagcttacgg cgatcctttt gaagatgctg
      481 gcatgcctca tatttatatg aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa
      541 acagcaagct tttgagaaaa atctctcact ggcttactgt tcttttttgt gtttacttac
      601 gcgacaattg cttgtaacgg ttataccaaa tatcaatata gccttccgag aacgggccat
      661 ctccattatt gatccagtca accagaatct tcacattttc ttttaggatg aagtcaatat
      721 cgttcgggta attcatcatt ttgctgtgca tctcatattc atcgacatcc aatagtcgtt
      781 tctcgccgtc aggaaatacc ttgatatcca agtcatagtc gatatatttc aaggcctcat
      841 cgtccagcaa aaaaggtgaa gctagattac aataatagga aacccccttt tctctgatca
      901 ttgctattac attgaaccag tattttttat gaaaataaac aatcgctggt tcgcgcgtta
      961 cccatcggcg tccatcagat tcagtgacta atgtgtgatc attcacgccg atcatagagt
     1021 gttcgcttgt ttttaatacc atggtatctc gccacgttcg atgcaatttg ccgtcgtgct
     1081 tgtaactttg gatcgtcaca aactctcctt cttttggaac tcccatagca acccttcctt
     1141 aagcagcatt ttgtgctttg tgtttgggtg ttcattacac cgacatcata attattatat
     1201 caataaaaat gagctttgcc tagaaaggat tcctattgtt tcgatttttc tctaactgat
     1261 ctaccagttt catttggacc ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc
     1321 acttcgaagt tttgttctcg gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa
     1381 aaaggagaac atgccatttc agatgactaa aaatatgagt aacttctcct aaatgtcgtg
     1441 tctgccaaac aaagagttcc tgcttttcaa atgggagatt ttgctcttct tctgctacta
     1501 ggtcatcaaa cagatcgatt tctggctttg tttcccattc ttttttcaat cgctcatact
     1561 cctcttgtgt cacttccatc attggaaatg tccacatatt cgccagcaat ttttggctat
     1621 ctctttcttc aaaataatac gctccagaat gattttgaag agctgcactg atataataaa
     1681 cgtcttttgg ctttgctttt tttgttttta caggaaaact cgtttggatt cctcgtttgt
     1741 tagctaaaca aaaggcttgg atcggacaag tctcacactt tggagaagtt ggtgtacaga
     1801 tagctgaacc tagatccatc attgcttggt tgaattcacc tggatgcttt tcatcaatga
     1861 ttttccgcat cgcttcatca aagattttcc tgctagaagc ttttgcgatg tctgcttcaa
     1921 tgcaaaacaa tctgctgact actcgcatca cgttgccgtc aactgcaggt tctggaagtc
     1981 caaatgcgat acttgcaatc gcgcctgtcg tatatggtcc tattcccttc aatgagctaa
     2041 tttcttcagg cgtttgcggc atttccccat caaattctga catgatctgt ttagcagcag
     2101 cttggatgtt ccgggctctt gagtaataac caaggccctc ccatgctttt aaaagctttt
     2161 cttctggagc atttgctagc tcttcaattg taggaaacca ttccatgaaa cgataaaaat
     2221 aatcgatcac tgtgtctact ctggtttgct gaagcatgat ctcagagatc cagatacgat
     2281 aaggatcacg attatagcgc caaggcaggt ttcttttttc ttgttcatac cattgaatga
     2341 actgatcttg aaattctttt gtttctttat ctgtccattt ttcccattct ttattcactt
     2401 tcctcttcct tttccgtaat gaatcgctgg atcatatcca aatcaaatcc tttttgatat
     2461 aagctttgct ttacttttag atttcttttt ttaggttcaa accttaaatt tcttcgccat
     2521 agtttatctc cttgtttgac taaataatca tattctattt cttgatctgc ttcttgcgga
     2581 agatcagcta tcgcttgttg gatcacttct tgagaaaagc ctttcgtcat caatgtttgt
     2641 tggatttttc tcaataattc tttttggctt tttccgtgat tttttccaaa cgctttttcg
     2701 gctgtctgat gtgcattttg aacttggtct tcaaatgtat attggctgag cgcttgttca
     2761 atgatttcag gtttcaaacc tttttgctgc atctgctgcg ctaatttctt tggtcctttg
     2821 tcacttaagc gcatattcgt gcgcacataa ctttccccat agaccaaatc atcgacaagg
     2881 tccaattctt ttaagcgggc tacgatttta tttcgatctt cttgaaggat ttccttttct
     2941 tttaagtatg ttcggatttc tttttccgat cgcaactgat aacttaagta attcattgct
     3001 aggtgaaaac cgaaatcagc tccgctactc ttcttgatct cttggatagt ttcttcgtct
     3061 aattcccgtc cttttaataa tcgatagcgt actaatacat cttcagatac ttgtaaagct
     3121 tctccttcag ataaatctac gcgatagaaa gggcctttgc ctttactgat tctcgtaacg
     3181 gttaacataa acatcctcct cgaacatata ttctctatca taccacagaa aatccccgta
     3241 atcaggtatt atatgttaga ataaaccaca gaaaaaagaa aaagggagat acttatggca
     3301 gaaacaattg tcaaaactgg acaaacggtt cctttgaaaa tcaagcgttt aggcataaat
     3361 ggtgaaggaa tcggttattt caaacggttg atcatatttg ttccatatgc actaccaaaa
     3421 gaagaagtac tagtgaaaat cacgaaagca actcctcgct atgcagaagg agaattaata
     3481 aaagtcaaaa aaacaagcaa agatcgcgta gtggcaccat gtcctgttta ctatgaatgc
     3541 ggtggctgtc aattacaaca cttagcttat caagctcaat tggattttaa aaaggatctg
     3601 cttttgcaag cattggaaaa attcaaacca gcaggctttc gatcttatca acttttgccg
     3661 acgatcggga tggatgagcc ttggcattat agaaacaaag cacaatttca gttacgaaaa
     3721 aataagcaga ctcaaaaaat tgaagctggt ttatacgaag caaactctca tcagttagtc
     3781 cctataacag actgcttggt tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt
     3841 caactattga ataaatttga tgcacccatc tatgatgaac ggacaaacag tggaatcttc
     3901 cgcacaatca tggttagaat cggtgttaaa actggggagt tacaagttgt ttttattact
     3961 cgcagtaaaa aattcccgca aaaaaacgca atgatccgtg agatcaatga acgactgccc
     4021 gaagttgttt caatcatgca aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat
     4081 gaaacgatcc acttatgggg aaaagaagcc attgaagaac aaatcaatga agtgactttc
     4141 gatctttctc ctcgtgcctt ctttcaactc aatcctcagc agaccgaatt gctttatggc
     4201 gaaggaatca aagcattaga tgtccagcct aatgaaacga tcgtggatgc ttattgcgga
     4261 gttgggacga tcggcttaag tgtagcaaaa caagccaaag aagtacgggg aatggatacc
     4321 gttcctcaag caatcgctga tgcaaaaaaa aatgcggaaa gattaggata tcacaacact
     4381 cgatatgaag tcgggacagc agagcaagtt ctgcctaaat ggttgaatga aggttttcag
     4441 cctgatggta tcatcgtaga tccaccacgt acgggtttgg ataacgcatt gatccaatca
     4501 ttattgaaat accctccaaa aaaacttgtt tatatctcct gtaatgtttc tacacttgcc
     4561 agagatctgg ttaatttaag taaaaagttc aagattgaat atttacagtc cgtcgacatg
     4621 tttcctcaga ctgcacgttg tgaagttgtc gtgaagatga cgagactcgc tgactaaatg
     4681 aaagaggttg tgaaaaagtc tgccctgtat caagtaataa gaacagccaa acaaaaatag
     4741 ctcttcatgt tttatgaata ctgtacttgt gaccgaagag ttaacaggag gacactattt
     4801 atttaaaaaa agggttgtga catagttttt gtcacagccc tttttgatta ttattggata
     4861 ttcacgtcta tcacttgata aaatgcattt gtcgtatcgc tgatattcca tactgctaaa
     4921 atcacattat atccttttcg atcctgcgga agtgtgatcg tttggttgac tgtttttggt
     4981 ggaacagttg ctttgtcgtc tatttttgtg atcaattcaa aatcactaaa tttcaaaggt
     5041 tgattaggat tccaagttgg tttggtaatg tagtaatccc aagtacttgt ccgatgttgt
     5101 gctgttaaat tccaagtaat atttaatgtt cctggattaa ccatgttttt atgccatctg
     5161 ttagatgtct gttcatctaa aggctcaaag ccgctgactc ctgcactagc aatttttcca
     5221 tcaatgaatg tatttttggt agcttcgata ctttgcggtt cccattgtgc tcttccgaca
     5281 ttttgattta agtttcctgc tgctgatgtt cctaggtatg cacggcttcc tggagaagta
     5341 atataaccat gcgcaaaacc tggaatgcta aaaccgaaca accctaaact aactaaccca
     5401 ataaacaata acgtactaat ctttttcatg tagaaacctc cttcatttga taaccacatt
     5461 gtaaacgctt tattgtatta acacaaacat tatgctttta taattttatg ttgaagtaaa
     5521 aaaggtgaaa aaagtaagat gcagattata acagaagtag acgagaaaag tacaaataga
     5581 aaacatgaga atcaaatcat atgatctttt tcaaaaacat cattcatata aacaaatgtt
     5641 caacttttta ataacaacaa ttatcaattt ttttcttact tcacaaacta aatacaggtc
     5701 ttgctgtgag ccttcatggt aaactaatat tatagaaagg agttagtaaa atgaagatga
     5761 taaaagatat tcgtttttgg gtttgtgtta taggaattgt catattaggc tttttatcag
     5821 ggttgcttag cggaaatccc ggaaaatatt actattcttt gcaacttcca ccatttgcac
     5881 cacctagctg gatatttggg ccgatgtgga ccttactgta tatattgatg ggtatttctc
     5941 tttatttgtt acttaatcac aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg
     6001 tgattcagtt catttttaat tttatatggt ctg
//
LOCUS       DAZKLA010000142         6011 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000142 DAZKLA010000000
VERSION     DAZKLA010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.144"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..684)
                     /locus_tag="ACG85N_002530"
     CDS             complement(37..684)
                     /locus_tag="ACG85N_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0225244.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(692..1378)
                     /locus_tag="ACG85N_002531"
     CDS             complement(692..1378)
                     /locus_tag="ACG85N_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0225245.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(1384..2457)
                     /locus_tag="ACG85N_002532"
     CDS             complement(1384..2457)
                     /locus_tag="ACG85N_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0225246.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            2679..3353
                     /locus_tag="ACG85N_002533"
     CDS             2679..3353
                     /locus_tag="ACG85N_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0225247.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            3358..4698
                     /locus_tag="ACG85N_002534"
     CDS             3358..4698
                     /locus_tag="ACG85N_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0225248.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(4847..5833)
                     /locus_tag="ACG85N_002535"
     CDS             complement(4847..5833)
                     /locus_tag="ACG85N_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFQ0225249.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         1825 a         1133 c         1055 g         1998 t
ORIGIN      
        1 aaaaatttca ggacaaatac acatagctgt ttctttttat tgtattcctt cagcaatcca
       61 acgagattta tagcgaaagt cttttaacaa ctcttctttt gtccaattat ttgcaatgag
      121 atccgtcaaa gtgttcatta cgagggtcat ggaaaagtat aggaaactaa ctgcttcttc
      181 cgacgattga atggagaaat gattgattct taataattcc agccattttt cgttcattgg
      241 agaatcagcc gatagttctc tacgtttggt tgtctgctga tcatttcgct gggttaacaa
      301 gtataacctt tgccaataga tactttcgtg atctaagtcg cagcaccact ctaaatattt
      361 ttcgatcccc aaaaagaaat gttgtttgtt ttcgctaatg aattttaaga tatcttgatg
      421 gactatgtga gaactgcttt tgatcgtata caaataagca tcttccaaat cttgaaaata
      481 tttataaaaa gcaccgcgcg acatccccat cttgtcaacg atttctgaga ctttgacttg
      541 gctgatgtgc tgtgagcaaa atatctccag taataggtca tcgattctct gtttctttgc
      601 gttcgataaa ttgaaaaaag tttcttttgg catccacatc tctcctttat ctctgattat
      661 acgtggatag cccttcattg tcatgaagga ttcattcctt ctttttctct tcttctgtca
      721 agtacccatc ttccattcga taaacgcgat cactccattg aatcatacgg acatcatgag
      781 taaccataat agtcgctttt tgtttctcat gagcttcttt aactagtaat ttgacgactt
      841 catatgcatg atctgtatcc aaactagctg ttggttcgtc tgctaagatc aagcttggct
      901 cattatataa agcgcgagca attgccactc tctgtctttc tccaccagaa aggtctttag
      961 gataactatt tttaagtgac tggatatcta aagattgcag caaggaatcg atttttgttg
     1021 aatcttcttt ttttgaagta acttggtcaa ctagttcaaa ttgttccttt accgttaaaa
     1081 aaggaatcaa attagagctt tggagaataa atcctatttc tttaaagcgt aaatctgctc
     1141 gttttttttc tggaagctca gttagtgagt gaccgttgat cactatttct ccggaagtcg
     1201 gtgtttgcaa tcctcctgaa atggtaagga aagtactttt acctgaacca gaaggaccaa
     1261 tgatgctgac gaattctcct tcctttacat aaaaatcaat tgctttcaaa gctgagacct
     1321 ttgtatgacc agaaccaaat gttttggtaa cttttttcat cgttaatatt tttttcattt
     1381 ttattaacct cctattgctg taattgggtc tactttggtt atcgtacgta tcgaaaacaa
     1441 tccgcctagc attgcgacaa tgatcaaaat cccactatat agcaaccatt gtgaccaaaa
     1501 aacagcaaaa ggcatcgcgc ttggtaaaat cagactagtc aaataagcaa acaaaagagc
     1561 aagcagaacg cctaccaccc caacaataaa ggactgtgct actaaagatt tagcgatgaa
     1621 gaaatttttg atcccttgtg ctttcattac tccaaaaata gcagtcttct gaagtgtcat
     1681 cacgtacatg aaaataccga ccacggcagc aaccactaag aacaaaaagt atatcatcgc
     1741 atttaaggtc atattttggg cagaatagcc aggaatactt tcaatgaatg tcccaatagt
     1801 taataactgt agacccttcg atgctttttc tttcgaaatt ttagctgaat cctttattac
     1861 gatcccattg atcggctgtt cttcttcaga agcaaacggc tgttgtccat acttcaaagc
     1921 agtccacgta tctagggacg catagatgac gggagaaact gtgtaataag tttcaggaaa
     1981 gataccgaca atcgtcaaag gatcgtcgta actgccaatt ttgatcgtat ctccaatttt
     2041 atagcctgaa tcagccagat tttgagagat gatgatttca ttctttgttt taaattcatg
     2101 ccctttcgtg agttttggta ataaaaatgc ttctttcgtt gtgccgaaaa ctgtaacatt
     2161 ttctttgttt tttcctttga ttgctccgct gtacaatccg attggtgctt tctctcctcc
     2221 ttcaatgtat ttcagatctc ctcttgttaa ttgtgaagct gcaaatactt gatttgcatc
     2281 ctccgacaaa acaatctctt gtgcgtccca atcatcgatt gcttttttaa actcttctga
     2341 aagtccattc gctagtcctg acaacatgaa tacgacatac gcaatcaaga acataatacc
     2401 gacgatcaag ccatatcgta atttagaata gcgaatttct ttgatagcta gaaacatttg
     2461 tttttcctcc ctatgataga ttgatagtga caaaatgtca cttgttgaat agtgtaactc
     2521 taaagtgaca atttgtcact tgtgttgttt ttttcttatc aataaattgt ctaagacatc
     2581 tttcgataaa accttaacat gagtcataaa aaatttattt aagccttatt tatgctttcc
     2641 atgataaact aaagtgacca aatcaatgag gtgggaaaat gattaaacta ttagtagtag
     2701 aagacgataa agtattatcc aataatataa aagaaatact cgctgcaatc ggcgagataa
     2761 cacaagttta tgatggtagt gaaggactgt tcgagttgga aagtggagtt tatgatcttg
     2821 caatcttgga tttgatgatg ccggaaatga atggatatga agtgttagca aaaatacgga
     2881 aagaaaaaat acagactcca gtattgattt tgactgcaaa agatagtttg gaagataaga
     2941 ttgaaggatt tgaacgaggt gcagatgatt atctaacaaa acctttctat cgagaagaac
     3001 tgatcatgcg cgtgaaagca ttattgaaac gctcgctagg tctctttgat gaaaatgttc
     3061 tgaagtataa ggatatcagt tgtgatttaa aaagcaatga agtcttgtat aaagaacaag
     3121 ttttgcccat acaaggaaaa gagtttgaat tgctcgttta ttttttacaa aataagggaa
     3181 tcattttgac aaaagagcag atatttgacc ggatctgggg atttgattct gatacgacga
     3241 ttacagttat tgaagtttat atgagcaact tacgaaaaca tctgcgaagt actggattgg
     3301 ataaagaaat caaaacctta cgtaatgtcg gttatatact gcaaagggtg tgatgcaatg
     3361 aatggagata ttacacgaaa gcaacgtagt cgcttttttg cgacaaatgt agcagcattt
     3421 gcttttatct tttttctttt gggattcatt actttacaga tattaaacca atctgcatac
     3481 agagaaacag atctgtcatt agagaaaata aattttgatt ctcgaacagt acaattagag
     3541 atacaaagat atcagcaagg cgatcccttt ttaaacccga ctcaaacgga acaaaacaga
     3601 acaaacatgg aacagccatt aatggaacca tcaaatgacc gttttaacac tcaagtgatc
     3661 ttgtggtcta aaaatggtga gatattgaat aaagcagcta ttggtgggcg tttcaaccaa
     3721 atcaaagaat taaaaatgga tacgaaaaat ctgaatacca tccaaaatat tgaattaaat
     3781 gaagacagtg gctcatctgc cctctctttc cgttcgatta cgaaaaaagc ggagacgaat
     3841 caagatggcg ttgcctacat ccaaatttta gaaaatacaa atcaaatcaa gaattcatta
     3901 gcgacttttc gcacgattat gattttgtgt atgattgttt tttggattct ctctattggt
     3961 atcagttatt acttgtctag tctaagcatg cgccctatta ttctttcttg gagaagacaa
     4021 aaagaatttg ttgagaatgc ttctcatgaa ttgcgcacac cgttaacgat tattcaaaat
     4081 agtttggaac atctttttac caagcctaat catactatta ttgaagaatc cgaaagcatc
     4141 gctcaagcat tgtcagagac gaggcgcctc actggattga cttcggattt gctgacaatc
     4201 gctcggaatg attctgatca gcagctgctt tcaaagcaaa tgatcaatac acaagaatat
     4261 atcgaacaac ttgttaaacc atttcaagag atggccatca tagatggaaa agagtttatt
     4321 ctccataacg atgcaacatt agaagtacca attgatgaaa aaaaaatcca tcaagttttg
     4381 gttattttgc tggataatgc attaaaatat acgaatccag aagataagat cattttggat
     4441 tcatctgtta accataaaga atggctgatt cacgtaaaga atactggccc ttctatcagt
     4501 gaagaagatc aagcaaggat ttttgatcga ttttatcggg aagatcaatc tcgggcgaaa
     4561 gagactggcg gatacgggct tggcttggca attgcaaaac aggtaatcga acagcatcat
     4621 ggacagattt ctgtatccaa ttatcttcca caaggagtcg attttaaagt ccaattgccg
     4681 ttgaagccta aaaaataatg cttatcccta aaaaagggct gtgacaaaaa tcttgtcaca
     4741 gccctttttt gttgcgaata aacggtgttc cagaagtagc ttcttcggaa ataagccgaa
     4801 atttctttaa gctgaccact tctgtcacaa cctctttagc ttacgcttac gattttttat
     4861 tgcggtcttc aatgtgtaca tcaccgcttg tgtcaatatc caattcttct cggctaacat
     4921 cttctgtcac tttttcaaca tcttcatgtt cttctttatg aatcccaact tcttcacgaa
     4981 taactgtatg tttgcttaca tctacttgct cttctttgat tgggatagta atcgtgtcat
     5041 cttccaattt ttcgtctgtt ccctgtgaag attgatctgt tactggtttg cgttcgatga
     5101 cgatttcttc acgtttgacc ggtacttcta ccgtttcagt atcgttgacc acatgtttgt
     5161 ggatgtcgac ttctccagtt gtcacatcat gtgtatttac atctaatttt tcttctttta
     5221 gttgaattgt atcatcatct agatttggtt tgcgatcgat gtcttcagtc ttgcttgtac
     5281 ccattgtatc agtctcgcca ggtacgtcca tcggatcagt tcctggtgtc atattcaatg
     5341 gatccgttgt attttgaaag cctgtcgtgc cacctacgtc cattggatct gttccagtgc
     5401 cagtttctaa tccagtcccc attgtgcttg ctgggctaac agtatcttgt gtatccattg
     5461 tatcttctgt tgtttctggc tgacgatagc cttttactgt gatgacaaat tttccatcag
     5521 aaatatctcc tctatattga tataaaggat cattttctgg tgtagccgtc tcagaatcat
     5581 aggtatcaaa agaaaaagta tcttttattt tttcccataa agaacggtct tcttcaccat
     5641 ccatccgatt ttcatctgtt tcaacatcta taccagacaa tccgtcaaat cctagtctag
     5701 atgctgcttc atcagtagta taaataacga tatcatctcg ttcataccct tcgttacgca
     5761 accgttcaat tactgataat gcttcttccc tcgtactata gcttccgatg accgttaaat
     5821 ttttctcttc catgtaaatt cctcctagca aatagattta tgattggtat attgttactt
     5881 ataaatttat tctataggag acgattgttt tctacaaata tttagattgg aatttgatta
     5941 aaacaattta attgaaattt gaaaacattt atgttaatga aagtcatatt ctaataaaat
     6001 gtcatttgtt t
//
LOCUS       DAZKLA010000143         5961 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000143 DAZKLA010000000
VERSION     DAZKLA010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.145"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..317)
                     /locus_tag="ACG85N_002536"
     CDS             complement(<1..317)
                     /locus_tag="ACG85N_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatA-like O-acetyltransferase"
                     /protein_id="HFQ0225250.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESD"
     gene            complement(447..1385)
                     /locus_tag="ACG85N_002537"
     CDS             complement(447..1385)
                     /locus_tag="ACG85N_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0225251.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            1876..3093
                     /locus_tag="ACG85N_002538"
                     /pseudo
     CDS             1876..3093
                     /locus_tag="ACG85N_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            3227..3466
                     /locus_tag="ACG85N_002539"
     CDS             3227..3466
                     /locus_tag="ACG85N_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0225252.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            3530..3583
                     /locus_tag="ACG85N_002540"
     CDS             3530..3583
                     /locus_tag="ACG85N_002540"
                     /inference="COORDINATES: protein motif:HMM:NF018061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFQ0225253.1"
                     /translation="MRNVDKTSTVLKQKSSP"
     gene            complement(3621..4793)
                     /locus_tag="ACG85N_002541"
     CDS             complement(3621..4793)
                     /locus_tag="ACG85N_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFQ0225254.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
     gene            4811..4930
                     /locus_tag="ACG85N_002542"
     CDS             4811..4930
                     /locus_tag="ACG85N_002542"
                     /inference="COORDINATES: protein motif:HMM:NF018061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFQ0225255.1"
                     /translation="MFLWLKTLYRKRAVTWPFIFYTIIWTLSKQTKNSDYADK"
     gene            5055..5792
                     /gene="erm(B)"
                     /locus_tag="ACG85N_002543"
     CDS             5055..5792
                     /gene="erm(B)"
                     /locus_tag="ACG85N_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0225256.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT         2045 a         1030 c          987 g         1899 t
ORIGIN      
        1 ttatcagatt cagtccaaat gttagtaaat ttttcagttt gcttattaaa aactgtatac
       61 aaaggattta acttatccca ataacctaat ttattctcac tattaattcc tgttctaaac
      121 actttatttt tatttacaac ttccataatt gcataaatta aagagggata aatttcatat
      181 cctttctttt ttatcatatc tttaaacaaa gtaatatcaa tttctttagt aatgctataa
      241 gtagtttgct gattaaaata gtgttcaaaa tattcttttc tatcccaatt ttctaattca
      301 ataatattaa aagtcatata taacttcctc ctaaatttta aatttttata tttaggagga
      361 ataatcctct gattttttca tacgttatgt cacctcgtaa atattaatta tactgaatta
      421 gcaattttta tcaaataaaa cttattttac ttccaaaacc taaattcacg ttgccaaaaa
      481 tcaatctgct tttgcaattg tttttcgttc gcttttaaag tcgatttcat taattccgtt
      541 aaatcaattg gagatatttc tctaatcaat tttttaaatt tagtcttagt attcttactt
      601 agctttcccc acatactttc ttcatgcaac aaagtataaa ccatagcttg ctcattaatt
      661 ttttctaaag tagcccacgc aggtttcaag atgtgtaaat cattaaaaca atcattccag
      721 taatcaacca tatctctttt taattcaact tctacacgcc ataaatgttc agacacaact
      781 tcaacatctg cgttatcttt acgttcttgt tttttattat aaattctaat aaatctatca
      841 ctatcacgga caccaaaata ttttgtttct ggcttgccat tacgaccata aaaaacagtt
      901 ttcttaactg ctttatcagt cattgcatag taatcgctca aatcatcttc aaaatcaaaa
      961 gctaagtcta atcttgtaaa accgtcatct tccatgtagt cgataatatt ttgttttaac
     1021 caaatcattt cttcatgtgt gagtttattg ggattaaatt caacacgcat attacgtcta
     1081 tcccaagtat ctgcttttac tttgtcatat tcgatataaa ctttttcttg aagtgcctta
     1141 gctttaaact ttgtttgaag tatatcccaa agtcgtattt gtggctctac actcataaag
     1201 tcagatagct ttttagcatt agttttgttc aaatttccaa cgattgtcat ggcatcaaaa
     1261 cttaatgcgg gttgagattt tcccaaagtt tgaccactta accggctatt acttaaccgg
     1321 ctattagaga cggccggttt ttttagtgag atttctgcag ctaatttttc atcaattttc
     1381 ttcatttttt atcgctccta aaataatttt tggaacgaaa aaaggacgag tggaatcgca
     1441 aaataaattt cgcctgtatc cattcgtcca ttttgttttg tattcaaaat caaaaattga
     1501 tatactaatc atcgaatgaa taacaagtgt ggtttgttat tccgggcgag aacgttggta
     1561 gcgttgctcg tcttttttta tgtctttttt tcgttaaaac tattttaaaa ctaaatatat
     1621 cttaaagcaa ataaattcaa ggggctaaaa gcgcccctta aaaacccctt gcctctgaaa
     1681 agttttgtgt ttcctttccg tcggtttcaa aagctaattt actcaaaaaa atagatcaag
     1741 ggtaaatgca ctcactgcgt tcgcccttga gctattttat ttcgcaaatt ttaacgcatt
     1801 tgccgacgaa aagataaaac acataaaaat atacgcagat atatttaaaa cgtcttagaa
     1861 cgcaaaattt tacgtttaaa agctatgcaa ggactgtatg aagcaaaatt attgagttat
     1921 ccaagaacag atacaccatt tattacagag aacgaatttg cttatttaaa agcgaatttt
     1981 ggcaaatata gcggtttttt aggacttgat cttgaaatgg ttcaaacaga gcctagaaag
     2041 cgttatgtgg acggtagtaa ggtacaggaa caccacgcca ttatcccaac aaaacaagta
     2101 cctaccgaat ctgcattagc gaaaatggac gatttacaac gaaaaattta tgctttagtc
     2161 gttaaaacga ccgttgccat gtttttacct gattacttgt atgaagaaac caagatacaa
     2221 acgaaagtag ctgatttgct gtttcaatcg atcggaaaga caccaaagca agaagggtgg
     2281 aaaattcttt tcaaacaaca aaccaaagaa gaaaaagagg acgttcaaac gttaccactc
     2341 gttatcattg gcgaacgtgc cgaagttggt gttaagagtg ttgaaaaaga aacgcaaccg
     2401 ccaaaagctt ttacagaggg tacattatta actgctatga aaacggcgaa taaaacggtt
     2461 gatgatgaag aagcaatcaa gattttacaa gaagttgagg ggattggaac agaagcgaca
     2521 agagcaagta ttattgaagc gttaaaacaa aaagaatata tccaagtgat taagaataag
     2581 cttgttgtaa ctgaaaaagg aaaattattg tgccaggcag ttgaaagtca gcacctttta
     2641 acgagtgctg aaatgacggc taaatgggaa acgtatttaa aaaaaatcgg taaaagagaa
     2701 ggcaatcaag agaactttat tacgaatatc aaaaaattca ttgttcattt actggaagct
     2761 gtacctaacg atatagaaaa actaaatttt tctgattacc aggaacagaa agaaaaagaa
     2821 gcagaaaaaa gtattgtagg aaaatgtcct aagtgtggca acaatattgt attaaaaaaa
     2881 tcgttttatg gttgttcaaa ttatcctgaa tgtaagttta ctttagctga acattttaga
     2941 aagaaaaaac taaccaaaac gaatgtaaaa gaattactgg agggaaaaga aaccctggta
     3001 aaaggaatca aaaacaaaga gaaaaagccc tacaatgccg ttgtaaaaat tggggaaaag
     3061 ggatatattg attttatatc tttctcaaaa taaacataaa agccctttaa agagggcttt
     3121 tatatattaa tcacaaatca cttatcacaa atcacttatc acaaatcaca agtgatttgt
     3181 gattgttgat gataaaataa gaataagaag aaatagaaag aagtgagtga ttgtgggaaa
     3241 tttaggcgca caaaaagaaa aacgaaatga tacaccaatc agtgcaaaaa aagatataat
     3301 gggagataag acggttcgtg ttcgtgctga cttgcaccat atcataaaaa tcgaaacagc
     3361 aaagaatggc ggaaacgtaa aagaagttat ggaaataaga cttagaagca aacttaagag
     3421 tgtgttgata gtgcattatc ttaaaatttt gtataatagg aattgaagtt aaattagatg
     3481 ctaaaaattt gtaattaaga aggagggatt cgtcatgttg gtattccaaa tgcgtaatgt
     3541 agataaaaca tctactgttt tgaaacagaa gtcaagtcca taatcctgtg taaaatacta
     3601 tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt agaactgagc
     3661 cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga cgttcggttg
     3721 ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg ttcaagaagg
     3781 ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa ggcatcttcg
     3841 aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat atattcaccg
     3901 actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa gatcgctttt
     3961 actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat gtttcttaaa
     4021 aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat cgcagacact
     4081 aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg ttcttttaag
     4141 tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat gaagccaatg
     4201 atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt agaaagcact
     4261 cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg ataattcgta
     4321 cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc agtcaggcta
     4381 gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt tgaaacttta
     4441 cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc cttttcattt
     4501 cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct aggcactttt
     4561 aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt tctttgactc
     4621 tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg ttgattttcc
     4681 atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt taccgaatgt
     4741 tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt catgtaacgt
     4801 cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg ttacatggcc
     4861 ttttatcttt tacacaatta tatggacttt atcgaaacag actaaaaaca gtgattacgc
     4921 agataaataa atacgttaga ttaattccta ccagtgacta atcttatgac tttttaaaca
     4981 gataactaaa attacaaaca aatcgtttaa cttctgtatt tgtttataga tgtaatcact
     5041 tcaggagaga ttacatgaac aaaaatataa aatattctca aaacttttta acgagtgaaa
     5101 aagtactcaa ccaaataata aaacaattga atttaaaaga aaccgatacc gtttacgaaa
     5161 ttggaacagg taaagggcat ttaacgacga aactggctaa aataagtaaa caggtaacgt
     5221 ctattgaatt agacagtcat ctattcaact tatcgtcaga aaaattaaaa ctgaatactc
     5281 gtgtcacttt aattcaccaa gatattctac agtttcaatt ccctaacaaa cagaggtata
     5341 aaattgttgg gaatattcct taccatttaa gcacacaaat tattaaaaaa gtggtttttg
     5401 aaagccgtgc gtctgacatc tatctgattg ttgaagaagg attctacaag cgtaccttgg
     5461 atattcaccg aacactaggg ttgctcttgc acactcaagt ctcgattcag caattgctta
     5521 agctgccagc ggaatgcttt catcctaaac caaaagtaaa cagtgtctta ataaaactta
     5581 cccgccatac cacagatgtt ccagataaat attggaagct atatacgtac tttgtttcaa
     5641 aatgggtcaa tcgagaatat cgtcaactgt ttactaaaaa tcagtttcat caagcaatga
     5701 aacacgccaa agtaaacaat ttaagtacca ttacttatga gcaagtattg tctattttta
     5761 atagttatct attatttaac gggaggaaat aattctatga gtcgcttttt taaatttgga
     5821 aagttacacg ttactaaagg gaatggagat aaattattag atatactact gacagcttcc
     5881 aagaagctaa agaggtccct agcgcctacg gggaatttgt atcgataagg ggtacaaatt
     5941 cccactaagc gctcgggacc c
//
LOCUS       DAZKLA010000144         5722 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000144 DAZKLA010000000
VERSION     DAZKLA010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.146"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(179..961)
                     /locus_tag="ACG85N_002544"
     CDS             complement(179..961)
                     /locus_tag="ACG85N_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225257.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1026..1862)
                     /locus_tag="ACG85N_002545"
     CDS             complement(1026..1862)
                     /locus_tag="ACG85N_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0225258.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1880..3289)
                     /locus_tag="ACG85N_002546"
     CDS             complement(1880..3289)
                     /locus_tag="ACG85N_002546"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0225259.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3303..5126)
                     /locus_tag="ACG85N_002547"
     CDS             complement(3303..5126)
                     /locus_tag="ACG85N_002547"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0225260.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1743 a         1246 c          943 g         1790 t
ORIGIN      
        1 ttatatatca acgtttaatg aaactatctc cgtttcattt cagatacaaa taaatatagt
       61 cgggctccaa atccgaaaaa aaataccccc agtagcttat cctaagctac tggagatgta
      121 taagtacggt catataacaa cggggtaggg taaataaatg aaaaagatgg aagaatcatt
      181 attcaataac ttttttaaat acttcgttgt aatcagttac ctgtccgttc ttaaatacca
      241 cagaataatc ttttttccca ttttcaagta accgaagtgg aatgtcttta ccaacttgct
      301 ctgtcactgc attactgata cctgttagat tagctaataa atctgataaa gattgagcat
      361 ctgtagaaat ctctgtatca gtatttatcg tgattccttt atcttcattt acaagcttcc
      421 aagtaggtgg tatagctgtg tatgtttcga tgaatgaatc aattctttca tctatcgtct
      481 tcccagtatc tacttctgtc tcattactct tagatgaatc gattctttca tccatcgtct
      541 gctccgtaac tgcttctgtc tcattattct caccggaaac tctcttcaca cccaaactta
      601 tatattgtcc atctttagtg tgcatagtta aaattcccac gcttttacta ataaattcac
      661 tataaccagt tctttcaata gagacttcaa tgtatttcac actatcatac tcaaaagaaa
      721 aatagacatg taaaagcatt ttgttgttta catctacata ataatccttt gactctactt
      781 gagaccttcc tatacggaat acagctttac tgtctgatat ctctatacca gcatccttat
      841 tattaatctg ccaatctcca agtagctctt ctggcaagct agtagtttct tgtgaacact
      901 ttaccacttc actggtagag gtcgaaacat tcacctccgc actgttagaa gaattttcca
      961 aggaatcagg agccttagca caaccaacaa gtctttatat attgtgcagc cgatattttc
     1021 ttcattcagt acacccttga aatatgcagt gttagataaa gcagttcgtc ctgatttgct
     1081 gaggcaccca tctcttcttg aacgtactcc ttgattttca aggccgcccg atactctttc
     1141 ggaaaacgct gcttcactga agccagcaaa aaatcatcct tctccggcaa ctcttccttc
     1201 ttaatcattc gggcaataaa aaactgtaaa tgcgtcacaa atcttaagta aagcatcgaa
     1261 tctttatcaa cctttttttc gggaaacaga tatcctagca tagccaagat atcctttagt
     1321 gctcttaccg agagcatggt ttcttccata tttgcttgtt cactttgggc atttacaatg
     1381 tgaaacgcaa tgtttccagc ctcctcttct ggcaactgaa cattcatttt tttgttgatc
     1441 aattggatag catagcagcc gatctcaaat tcttttgggt aatacttttg aatttcaatc
     1501 agcaggcgat tttgaaaaac aatgcctttt tgactgcgtt caatggcatg cttcaagtga
     1561 tcaaccaagg taaaaaatag acttttattc agcttaaagt caaacttggc gttggcataa
     1621 tcgataattc gcttgacgca aataattaga tcatccggag cagcctcaaa cagtttaatg
     1681 taatcctttt ttagagcatc agaaagtgaa tctaatttaa atactttttg gattttgtca
     1741 gccggaacca aatcgccttt ctttagttga aatccaatcc ctttccccat caaaattact
     1801 tctttgcctt gcttatctaa gcaaagaatt aaactgttgt ttaacgtttt aattactctc
     1861 attagattca ccgttctctc taagctaagc catttcttgc gattactttt tgataccaaa
     1921 agaagctttc tttttttatt cgttttaatt ctttaagatt ctcatcggtt cggtcaacat
     1981 atataaaacc ataacgctta ttgaagcctt gatgggtact aacaacatca atcgctgacc
     2041 aaggtaaata accaaaaaca tctacaccat ctgttaaagc tgcttgaata gccagaatat
     2101 gtttggctag ataatcaatt cgatcctggt cattaattcg tctatcttcc tcaactatgt
     2161 cgggtgcccc atagccattt tcagtaatta aaataggtaa atcatagcga tcgtacaatt
     2221 tcctcaatgt taatcgtagt ccagtagggt caactaccca gccatatggc gttttatcta
     2281 cataaggatt ttcagctgcc cggtagacac cttcttctcc caacatgatc tgttgatccc
     2341 ccgctctagc ggaaacatct gatccgtcat ttttgctggc agcgatggtg gcagttgaat
     2401 aataattaat cgcaataaaa tcgggtttac cggaggataa aattgcctga tcctctgcct
     2461 caattttagg atataatcca cgatcattca aatagttttc aaacaaataa ttgtattttc
     2521 cccgaactgc tacatctaag aaagaccagc ctctcaatgt ttcccaatta tgggctgcaa
     2581 ttgcatcttc tggactggag gtagcttgat acatagccgt taaattgatt gctggaccga
     2641 tttttccttt aagattcaac tgatgataca agtgaattac tctagcttga gctaataaaa
     2701 catggtgatt aatttgaaag agttcttttt ttgaaggcag ctctccactt ggtggcaaac
     2761 caatcgctcc aggatgaagg atcatcgtgt tttgctcatt aattgtcagc caatagttca
     2821 ccttgtcacc atactcagta agcaatacct gtacatagtc cacaaaataa tcgatagttg
     2881 ctcgatttaa ccaaccgccc tgctcctcta gaaaataagg taaatcgaaa tggtacatgg
     2941 tgacaatcgg ttcgattccc tgattcaggc attcatcaat tacttgatga taaaaagaaa
     3001 ttcccttttg attggtttta ttattttcaa tatccataat tcgcgtccaa gcgatggaaa
     3061 agcgaaaagc ttttaagcct aattctttaa acagctgtat atcttcttta tagtgatggt
     3121 aaaaatccga ggcaacttca aaattagcta ccccttctgt atgctgatac ttgtcaataa
     3181 tagacggttg tttcccatcc tcctgatagg caccctctgc ctggtaggca ctaatggagg
     3241 caccccataa aaacttctct ggaaatgctg aaagctgttt gtattgcaca aaaatcctct
     3301 ccttaactaa tttgcatcaa tggttcgtgt tcctttaagc ttccgcttgc ttcactaatt
     3361 cctgcgtaat tttcactgtt ggtcactacc acaatcactg tatcatcgta gccagcgttt
     3421 ctaatggcag cgaaatcggc gttgattaat aattgtcctg ctgacacctg atctcctgct
     3481 tttacaaata cttcaaaggg ttcccctttt aaattcacgg tgtcgatccc cacatggatc
     3541 aataattcaa taccttcttt agaacgtaga ccaatcgcat gtttagattc taaaatagta
     3601 atgacttctc cctcaacagg tgaataaatg ctgttttctt ctggatgaat ggctacgccc
     3661 tttcccaata gtcccttaga aaagacagca tcgttcactt cagaaatagg acacacggtt
     3721 ccttttaccg gattttcaac aatgactgct tcggctattg ccgtttgctc tgattcatca
     3781 gaagtatcgt caaatccaat tagataagca atgataaagg ttgcaacaaa ggcgattaca
     3841 cacgtaataa ttgcgtgaac aatattcatt ggattttctc caataaaggc tggtaaggct
     3901 gctaagccag gggaaacaaa ggcatatctg actaaaccgc tcaagccagc atacaaacca
     3961 gcaattgcac caccaatcat ggaagcaact aatggttttt tcagcttcaa ggttactcca
     4021 tagagtgaag gctcagtaat tcccattaaa gcagtgatcc cagtggacac agctagctgc
     4081 ttcaactctt tgtttttagt tttcgcggct acagcaaagg tagcacctga ttgagcaata
     4141 ttagaagcca gcattcctgg cccattgatc atttcagaac ccgtactgct caattgcata
     4201 gtagcgattg gcgtcattgc ccaagctgta cccgtaacca ccaaaaatgg ctgcaaacca
     4261 cccatcaaaa atggaattaa ccaagctgct ttgccgttaa tccattcagc ccccattgct
     4321 ataagatcgt tcagcaacgt tccaaacggt ccaatgacaa ccaacgctaa aggccccata
     4381 attaaaaata ccagcatcgg cttagtaaag aatttaataa acgaaggcga aattcgatcc
     4441 gcaaagggct ctacatagga catgatccaa acagtaaaga taatcggtaa aactgatccc
     4501 gcataatctg cagctaatac cggaattcct agaaaagaaa cggcatcgcc agtagaaaaa
     4561 agctgtgaaa tatttggatg aagcaaaatg ccagcaacag taatcgccaa aattgggttg
     4621 acatcaaact ttatggctgc cccataggct aatagtactg gcataaaata aaaggctgca
     4681 tcagcaatag tattcaaaat gatgtaagtt tgactactgt ctgatagtac accagtcaaa
     4741 gtcaagacgg ccaaaatggc ttttaccatc ccggcacctg ttatagctgg aatcatcggt
     4801 gtaaaggtgg tagaaatgac actaatcaat cttgaaataa tactttcttg attgtctgaa
     4861 gaggggccct ttttaattga agttcccact tccttttgaa tttccttgaa ggctgtttgg
     4921 acttcattgc caatcaccac ttggtattgt ccaccgctat ccaccacact aattacacca
     4981 agtgtttttt ctaaggcttt cgtatcggct ttccttttat ctttgagttc aaaacgcagt
     5041 ctggtggcac aatgggttaa gctgaccaca ttttcttttc ccccaacctg ttgcaataca
     5101 tttcttccta cttctctgta atccattatt ctctctcctt aaagcagtaa aaaagaccaa
     5161 gtcaaaccac gccccaatca acattgaagt gttagtttgt cttggtcttg cctgcataat
     5221 cagtaacatg ccaataacgt tattttgctg taatccaata atagcacatt atgatagcgc
     5281 aatcaacagg gaatttaatt ttttatcttt tcgtttgacc gtatagggta atataacagt
     5341 tagatcacat caactagggt atatctgcgt atacttactg ctaaaataag tatatctaat
     5401 attaactata ccagtaaaac cattcctaca taaccagcgg gaaattccca aaatcgtgaa
     5461 agcatcaaaa atgcatataa cggatcacac aacttcaaca attctgctct tgggaaaaat
     5521 tgatgaattt ggtgagtaaa ggatggaata aacagcattc caacaatata ggcaacaata
     5581 gtgatacttg tttttttggt aaaaatcagt aagcaatagc caatttcaat cattccgaga
     5641 tataaaggta tcgatagaat gatttgggct agtattctta aaaattaact agcaaatgtt
     5701 taaactgatt tatttctaat ct
//
LOCUS       DAZKLA010000145         5689 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000145 DAZKLA010000000
VERSION     DAZKLA010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.147"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(488..1297)
                     /locus_tag="ACG85N_002548"
     CDS             complement(488..1297)
                     /locus_tag="ACG85N_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0225261.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="ACG85N_002549"
     CDS             complement(1297..2043)
                     /locus_tag="ACG85N_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0225262.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="ACG85N_002550"
     CDS             complement(2061..2528)
                     /locus_tag="ACG85N_002550"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0225263.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="ACG85N_002551"
     CDS             complement(2528..2938)
                     /locus_tag="ACG85N_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0225264.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="ACG85N_002552"
     CDS             complement(2949..3962)
                     /locus_tag="ACG85N_002552"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0225265.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="ACG85N_002553"
     CDS             4179..4913
                     /locus_tag="ACG85N_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0225266.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="ACG85N_002554"
     CDS             4897..5607
                     /locus_tag="ACG85N_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0225267.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DAZKLA010000146         5644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000146 DAZKLA010000000
VERSION     DAZKLA010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.148"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..1463)
                     /gene="lpdA"
                     /locus_tag="ACG85N_002555"
     CDS             complement(57..1463)
                     /gene="lpdA"
                     /locus_tag="ACG85N_002555"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFQ0225268.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            complement(1471..3114)
                     /locus_tag="ACG85N_002556"
     CDS             complement(1471..3114)
                     /locus_tag="ACG85N_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFQ0225269.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            complement(3139..4116)
                     /locus_tag="ACG85N_002557"
     CDS             complement(3139..4116)
                     /locus_tag="ACG85N_002557"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFQ0225270.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            complement(4119..5228)
                     /gene="pdhA"
                     /locus_tag="ACG85N_002558"
     CDS             complement(4119..5228)
                     /gene="pdhA"
                     /locus_tag="ACG85N_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFQ0225271.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            5390..>5644
                     /locus_tag="ACG85N_002559"
     CDS             5390..>5644
                     /locus_tag="ACG85N_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225272.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
BASE COUNT         1430 a         1276 c         1050 g         1888 t
ORIGIN      
        1 tctaaaaaac aaaacgcaac tgatttttca gttgcgtttc gcctgtatta ttcaaatcaa
       61 atatggattg gtaaacctaa tgctaattcc gaagcatcca tgacgatttc gccaagagat
      121 ggatgaggat gaatcgtcaa ggcgatatct tcagcattca ttcctgattc gattgctaaa
      181 gctaattcag agaccatatc acttgcgccg actccaccga tttgcgcgcc aataagcacg
      241 ttatcttcga tcgtcgtaac tagacggatg aatccttctg ttttaccaag cgataatgcc
      301 cgaccgttcc ctgaaaatgg gaatttatat gcttttgctt cgataccagc atcttttgct
      361 tctttgatcg tcatacctac agaagccaat tcaggatcag tgaaggctac tgccggcatt
      421 gctttataat ctacagctac ttttttgcca gagattgctt cagcagcgat ttttgcttca
      481 taactggctt tgtgtgctaa tgccgcccct ggaacgatat caccgatggc atagatgtta
      541 ggtacgttcg tacgtccttg tttatccacc gtgatcaatc ctcgttctcc tacttcgaca
      601 cccgcttgtt ctaagcccat atcatctgta ttcggacgac gacctacagt gaccatcaca
      661 taatcagcag taacggattc ttctttcccg tccactgcat atttcactgt cacgctgtca
      721 ccgttatcga ctgcttcttt cgccattgca ttcgttacga ctgttacgcc ttttttcttg
      781 aaatcatctt ctactaattt tactagatct ttttcgtaag ttggtaaaat ttgtggtgag
      841 ccttcaagaa tcgttacttc tgctcctaag tttgcgtacg ctccgcccaa ttcggcacca
      901 atcacaccgc caccgatgat cacaaatttc ttaggtactt ctttcaatgc taatccgcca
      961 gtagaatcta acacacgtcc tccgaattta aatcctggaa tctcgattgg acgggaacct
     1021 gttgcgatga ttgcgttatt gaacgaataa gtttgagctg aatctggatg aatgacgcgt
     1081 agtgtgtgat catctacgaa gaacgcttct ccttcgattg tttcaacctt atgttttttt
     1141 aataggaaac caacaccaga agtcaacgta tggacaactt tgttttcttt ccattcttgt
     1201 gtttttgcaa aatctagttt gacgttttca ctagttactc caaacatttc agaatctagt
     1261 gactcttgat aatggtgtcc tgcagctatc agtgcttttg aaggaataca gccgacattc
     1321 aaacaaacac cgccaatata ctctctttca atgattgcta ctttttgtcc catttcagca
     1381 gcgcggatgg cagctacata gccaccaggt cccgcaccaa ttacaactgt atctaattct
     1441 acagcaaaat ctccaactac catttgttta tcatccttcc atcattaata gctctggatc
     1501 agctaaaaga cgtttgatat tattcattgc ttgttgcgca gttgctccat ctacgatacg
     1561 gtgatcgaaa cttaatgaca gtttcatcac acgaccgaca acgatttcgc cttcagcatt
     1621 tacgattggt tgttgtgcaa tcgttcctac acctaaaata gctacttctg gatagttgat
     1681 gactggtgta aaccaaccgc cacctacaga tccgatattg ctgatcgtaa ttgttccatt
     1741 gcgcatatct tctgcagata gtttgccgtc atgagctaat tttgctttct cattgatttc
     1801 gtctgcaata gcaaacattc ctttgcggtc tgcatctttc acatttggta catatagtcc
     1861 gtgatccgta tcagttgcaa taccgatatt gtagtaatgt ttataaacga tttcttggtt
     1921 tgcatcgtcg atagatgcat tcaatactgg gtatttctta acagtcgctg ttaaagcttt
     1981 cacgacatat ggcaagaaag taagtttcgt accatttgca gcagcaactt ctttaaatcg
     2041 tttccgatta tcccatagtt tagacacttc tacttcatca tgcaatgtca cgtgtggagc
     2101 tgtatgctta ctgttgacca ttgctttagc gatagcttta cgtgttggcg tcattgctac
     2161 acgttcttcc aagtctccta agttggactt gaatggtttt gctggtgcag ctggtttgct
     2221 ttctgctgca ggagctgctt ctttcggtgc tgccgcttct ggcgcttctg atttcgctgg
     2281 tgcactgctt ggaccacctg ccaagaagtt ttcgatgtcc tcttttgtta ctctgccacc
     2341 ttttccagtt gcagttactt gtgagatatc tacatctttt tcacgggcaa attgacgaac
     2401 agatggcata gccaatacac gtttatttgg atcggcagct tcaacgacac ttgctgaacc
     2461 tgatgtttcc actttttctt ttggtgcttc agctgatggc gcactagttg acggtgcact
     2521 attgtgtcca ggagcatcga tttctactaa tacatcccca acatttgcta cagtgccttc
     2581 tggaacaatc acgtttttaa ctgtccctgt gacaggtgac ggaatttctt ctactgattt
     2641 gtcattttga acttctagta atgtatcatc ttcattgatc gtatctcctg gtttgacgaa
     2701 ccatttgacg atttcacctt ctgcgattcc ttcaccgata tccggtaact taaattggaa
     2761 gactccttcg cttgacgatc cagctgattc tgtatcaaca gtgggttcag cagccggttt
     2821 agctggagtt tgggattctg cggctacacc actgtcccct tcattgtctt cgtgtcctgg
     2881 tgcatcgatt tctactaata catccccaac atttgctacg gtaccttctg gaacaatcac
     2941 atttttaacc gttcctgtaa caggtgacgg gatttcttct actgatttgt cattttgaac
     3001 ttctagtaat gtatcatctt cattgatcgt atctcctggt ttgacgaacc atttgacgat
     3061 ttcaccttct gcgattcctt caccgatatc cggcaattta aattgataag ccatgacttt
     3121 gtttcgtccc ttcttttctt aaaattccgc gatttctttt actttgtctt cgatatcttt
     3181 tgcatttggc aaccagacat tttctgcttg tccaaatggg aagactgtat caggtgcaga
     3241 aactcgtccg attggggctt ctaatgaaag gatagcgcgt tctgagatct cagaaactac
     3301 ttgcgcggca acgcccgctt gtcgttgtgc ttcttggaca acaactacgc gaccagtttt
     3361 ttccacggat tgaatgatcg tttctacatc taatggcgca actgtacgaa gatcaatgat
     3421 ttcaacagat atattttctt ttgcaagatt gtctgctgct ttgattgctt cacgcaccat
     3481 tgctccgtat gtgataatag atacatctgt tccctcacga gtaactgctg ctttatccaa
     3541 aggaacttcg taagcttcat ccggcacttc ttctcggaat gagcgatata gtttcatgtg
     3601 ttctaggaaa acaaccggat cgttacttct aatagaagaa atcaatagtc cttttgcatc
     3661 ataagggttg gaaggaatca ctactcgaat accaggagac tgagcgatca aaccttctaa
     3721 attatccgaa tgcaattctg gtgtatgaac accgccacca aatggcgaac gaattgtgat
     3781 gggtagattt ctagtgccgc tcattcgata tcttgtacga gccatttgag ctactacttc
     3841 gtccattgct tcgaaaataa aaccaaagaa ttggatttca ggaactggac gaaatccttc
     3901 taacgcaaga ccgaaactca atcctgcgat tccagattca gctaaaggag tatcgaatac
     3961 tcggtcttct ccgaactttt cctgcaatcc ttcagttgcc cggaaaactc ctccgttttt
     4021 cccaacatct tcaccaaaaa caacgacatt ctcgtcattt tctagttcaa gtgctaaggc
     4081 atctgtgatt gcttggatca ttgttttttg tgccatgatt atttcgactc cttcgcttca
     4141 taaattgcaa tttgttcttt gattgattgt ggagatactt cgaacatgtt tttcaagaaa
     4201 tctgaaacct tttgttttgg tactttgtcg gcttcagcaa tagctgcttt gatttcttct
     4261 ttagttgctt cgatgacttg ttcttctttt tcttcagacc aaagtccttt ttccgtcaaa
     4321 tatttacgga agcggatcaa tggatctttt ttctgccaat cgtcatctgt gtcttttgta
     4381 cggtagcggg ttggatcgtc accagatagt gtatgtggtc cataacgata agtcagtgtt
     4441 tcgattagga ctggtccatt tccactgatc gcccattccc gtgccatttt agatacagta
     4501 tatactgcaa gcggatccat tccatcgact tgaataccag gaatcccagc tgcgacagct
     4561 ttttgtgcaa gtgtttttgc tgcagtttgt ttttcacgag gtgtagagat cgcatacccg
     4621 ttgttttgga tataaaatac ggcatttgca tggtaagcgc cagcgaaatt gattgcttcg
     4681 taaaaatcac cttgtgatga accgccatca ccagtataag tgaagacgac atttttcttg
     4741 tttcgtttct tcaaacctaa tgcaacaccg gctgcttgta catattgtgc accgatgata
     4801 atttgtggtg gtagagcttt caattcttcc ggataagaat tacctgcagc atgtcctcga
     4861 gaccataaaa aagcttgaga taaaggtaaa ccatgtttta ctagttgagg tacatcccga
     4921 taacctggaa gaagaacatc ttctttatca aatgcgtaag cactagccag ctgacttgcc
     4981 tcttgtccag cagttggtgc aaaaaagcct aggcgtcctt ggcggttcag cgctgttgaa
     5041 cgttggtcta atacacgaga ccataccata tgagtcatta attcaacgag ttcatcatcg
     5101 gataaatcag gtactaaatc tttgttaact acgtttccat cttgatcaag tgcttgatat
     5161 actgggaaat ctgcattgac ttcatctagc agtgcttgga agtcgatcgg tgcttttttg
     5221 tttgccattt gtcacacatt cctctctcat ctataagaaa atctcttcat tctgcgattg
     5281 aaacagtgaa taaaactgta tcactattta accgtcttca ttaataaatt atcatggatt
     5341 tatgagaaat acaagctaaa agcacaaatt ttcacgatga aaggaaatta tgccaatccg
     5401 catattaaca gcattagtga ataaaatcac aataaggaaa gcgttttata tttcatctgc
     5461 attttttcta ttttctaatc aaacttacaa ccatctgttt ctatttaaaa acgttggtgt
     5521 tatacagttt tgttgcaaac atttatttta tttacacatc gtgaacggac gtacaaataa
     5581 tggttttttc ttagtttctt tatatcgtat tctttttccg aaaaaaaaga aacagcttga
     5641 aatt
//
LOCUS       DAZKLA010000147         5591 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000147 DAZKLA010000000
VERSION     DAZKLA010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.150"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      114..211
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            327..908
                     /locus_tag="ACG85N_002560"
     CDS             327..908
                     /locus_tag="ACG85N_002560"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFQ0225273.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            911..2218
                     /locus_tag="ACG85N_002561"
     CDS             911..2218
                     /locus_tag="ACG85N_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFQ0225274.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            2634..3122
                     /gene="purE"
                     /locus_tag="ACG85N_002562"
     CDS             2634..3122
                     /gene="purE"
                     /locus_tag="ACG85N_002562"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFQ0225275.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            3115..4239
                     /gene="purK"
                     /locus_tag="ACG85N_002563"
     CDS             3115..4239
                     /gene="purK"
                     /locus_tag="ACG85N_002563"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFQ0225276.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            4252..5547
                     /gene="purB"
                     /locus_tag="ACG85N_002564"
     CDS             4252..5547
                     /gene="purB"
                     /locus_tag="ACG85N_002564"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFQ0225277.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
BASE COUNT         1739 a          956 c         1300 g         1596 t
ORIGIN      
        1 gtttcagaaa gtagtgttag actgttttca atatttgaaa caacattttt tattcaaata
       61 tggcaaactg ccttcacgtg caaaacagtt gcaagaaaat gaatttacac tattattagt
      121 tttacatacc tacttatata tcgtcataat atggatgaca gtttctagcc agtaccgtaa
      181 atgctggact ataagtaaaa gattggctat ttcacccttc ttttcttatg ataaagtgga
      241 gggtgttttt ctttaacaga aacatagtag gtttttgcac aaaagcggtt atttcggtat
      301 taataaatga taaaggagca aataaagtga aagaattagt tgaacgtatt caaaaagacg
      361 gacgtatatt aggcgagggc gtattaaaag tcgacagctt cgtcactcac caagtagacc
      421 ctgtattgat ggaacaaatg ggaaaacggt ttgcagaagt atttaaagaa caaggaatca
      481 cgaaagttgt gaccattgaa gcttctggga tcgcaccagc tttatttgct gcaagagaat
      541 tagatgttcc aatgatcttc gctagaaaag caaaaagttt gacgatggat gaagaattat
      601 tgacagcttc tgtttattct tttacgaaac aagtgacaag tacgatttct atttctcgta
      661 aatttttatc ttcagaagac aaagtattga ttatcgacga tttcttggca aacggacaag
      721 cggcaaaagg attgatcgaa ttatgccagc aagctggagc gcaagtagaa ggaatcggga
      781 tcgtcatcga aaagtctttc caagatggac gtcagttact tgaagaaatg ggattgagag
      841 tcgtttctct tgcgcgtatt gcttccttga cgaatggaca agttgagttt cttgaggagg
      901 acgcataatc gtggaaaaag aaacacaaaa tggaaaagct gcggttttag ggctgcagca
      961 tctgctagcg atgtatgcag gagcagtagc ggtgcctctt ttgatcggta caggactagg
     1021 ctttaatcaa gaacagatga cttatttgat ttcaattgat atctttatgt gtggattagc
     1081 gacattattg caattgacag ttaaccgatt tttcggaatt ggcttgcctg tcgttttagg
     1141 atgtgcgatc caagcagtag ctccactgat cttgatcgga accaatgaag gagtaggagc
     1201 tatctacgga tctattatcg cttctgggat ctttgttgtg ttagtttctg gtgtcttttc
     1261 taaaattaaa aaactattcc ctcctatagt aacaggaacg gttatcaccg ttattggttt
     1321 gactttgatt cctgtagcaa tcgagaaaat gggtggaggt aatgcaacag cagctgattt
     1381 tggtgacaaa acaaatctgc tacttgcatt tgtgacgatt ctcctaattg taggcgtgca
     1441 aatgctggca aaagggttca ttcgttcaat cgcggtattg attggtttag tcggaggaag
     1501 catcttagct gctttcatgg gaatgattga tctgagcgcg attggagatg cgccgttgtt
     1561 ccatgttcca caaccctttt attttggtaa accaactttc gacgtatggt cgattttact
     1621 aatgatcatc atttccatgg tcagcatggt agaatcaaca ggtgtatatt ttgcacttgg
     1681 tgacattact gggaaaaatg ttggagaaga agaattgaaa aagggatatc gtgcagaagg
     1741 acttgccgtt atcttaggcg gtattttcaa tacattccct tatactggct tttctcaaaa
     1801 tgtcggattg gttcaacttt cgggaatcaa aacaagaaaa cccatctatt tctctgcatt
     1861 cttcttgatt gttttaggat tattccctaa aatcggtgcg ttagcacaaa tcatccctga
     1921 gccggtactt ggtggaggaa tgttagtcat gttcgggatg gtggcagtcc aaggaatccg
     1981 tatgctgttg gaagttgatt tcagcaatga taaaaatcta ttgattgttg ctgtttctgt
     2041 tggatttggg ctgggtttca atgttacgcc gacattattc agtcaaatgc cgcaaacatt
     2101 gcaaatgttc actggaaatg gcatcgtaat gagtagcatt acggctatca tattgaattt
     2161 gatctttaat ggattgaaaa aagatgaaga aatattggaa gaagccttca agaagtaata
     2221 gagcaaaaga aaggttgtga aatcagcgtt ttgacctgat tgttagagaa acaaacggaa
     2281 aaatagttcc tcgtattttg ccgtttgttt gagctaatac cgagggtcag ctgattgaac
     2341 accgttgaag gaagaggtcg tgagcctgcc gttcatctcc gagtcacaag gaacaatttc
     2401 aaaaaaaagc ttctcatgtt tgttgaatat tgttccttgt gaccgaggag ttggcaggag
     2461 aacaccgttt atccataaaa aggaatgtga cctaactttt gtcacattcc ttttttttat
     2521 ctttaataaa cacgaacaat aaaatgtttt tctttgttaa cattcgaaat ttctttgaca
     2581 tcaaaaaaag tgattgttaa aatatgaatg tagaaaaact gggaggaaaa acaatgacag
     2641 caaaaatctc tgtgattatg gggagtactt cagattggga aacaatgaaa gaagcatgtc
     2701 aaatattgga tgaattcggt gtgtcttatg aaaaaaaggt cgtttctgct catcgaaccc
     2761 cagatttgat gtttgaatat gcacaaaacg ctcgaaaaaa aggaatcaaa atcattattg
     2821 caggagcagg gggagccgcg catctgccgg gtatggtcgc atcaaaaaca actttgccgg
     2881 ttatcggtgt accggtccaa tcacgaacat taaatggttt ggattcgctt ctttcgattg
     2941 tccaaatgcc tggaggagta cctgtagcta ctacagcaat cggaaaagcg ggagcaatta
     3001 atgctggact acttgcgata cagatgcttt ccatgtatga tttagaattg gaggctaaat
     3061 tggctgaaag acgttcaatg ttggctaaaa ctgttataga aagtagtgat caacttggtt
     3121 aaacctttgt tgcctggttc aacgatcggg atcgtcggtg gcggtcaatt aggtcgtatg
     3181 atggcgatta gtgcaaaaga aatgggcttt aaagtaggtg tgctggatcc tgtgagcgat
     3241 tgtccagccg cacaagttgc agattggcac attgaaagtg tctatgatga cacctttgcg
     3301 ctgaaagagc ttgccagaag aacagatgtc attacctatg aattcgaaaa cgtcagtgta
     3361 gaggcgttaa atgcgatttt acccatgtca tttattcctc aagggacaga cttgcttgcg
     3421 attacgcaag accgcctgtt agaaaaatct tttttggaaa cgaataacat cgtgattgct
     3481 ccttatgcaa cgatcgtcag tccgacggat atccaagatg cgattgacgg gatcggctat
     3541 ccttgtgttt tgaaaaccac tcgaggcggc tatgatggca aggggcagta tgttttgaaa
     3601 agtcgggcgg atctagctcc tgccatggac cttttaagag aaggaacctg tgttttagaa
     3661 gcatggatcc cgtttgaaaa agagatttcg atcatggtgg caggaaatgg tcaaggagat
     3721 ttcacgactt ttccggtcgt ggaaaacatc caccacaaca atattcttca tgaaacgatt
     3781 gctccagccg cgattgatca ggatgtgatc gaggaggctg agcgtattgc ccgtgtgatt
     3841 gcagaagcag tgagcctttc aggtgtttta ggggtagaaa tgtttttaac aaaaacaggc
     3901 gggctatatg tgaatgaatt agctcctcgt cctcacaatt ctggacatta ttctattgaa
     3961 gcttgttcta tgagtcagtt cgatgcccat attcgaggta tttgtggttg gccattagga
     4021 gaaaaagtac gtttgataag caatgcagta atggtcaatg ttttgggaga acaactgctg
     4081 gaaagctatc gcttgattga gaataagcca gactggcaat tccatttcta tggaaaaaaa
     4141 caagcaaaaa aaggtcgcaa gatgggacat atcactatct tgacagaaga catccagcaa
     4201 acgctggaag aaatcaaaca aacgaatatt tgggattaga ggagaaaaaa catgttagaa
     4261 cgttatacac gccctgaaat ggggaatatc tggacggaag agaacaaata ccgtgcgtgg
     4321 ctcgaagtcg agattttagc agatgaagct tgggcagaac ttggtgaaat cccgcaagaa
     4381 gatgtgaaaa aaattcgtga gcatgcatca tttgatatcg atcggatctt ggaaatcgaa
     4441 aaattaacac gccatgatgt cgttgcgttt acacgagcgg tttctgaaac attgggagaa
     4501 gaaagaaaat gggtccatta tggtctaaca agtacggatg tcgtagatac tgcttatggc
     4561 tatctcatca agcaagcaaa tgatatttta cgggaagact tgaaacgttt tacggaaatc
     4621 attggcgaaa aagcaaaaga acacaaatat acagtgatga tgggtcggac acacggtgtc
     4681 catgctgaac caactacttt tggattgaaa cttgccacat ggtattcaga aatgaaacgg
     4741 aacatcgaac gatttgaaca tgcggcaaaa ggagtagaag caggaaaaat ctcaggagca
     4801 gtgggaacgt tcgcaaatat cccgccattt attgaagagt atgtatgtaa acaattgggg
     4861 atccgtccac aagaaatctc tactcaagtt ctccctcggg atctacatgc tgaatatttc
     4921 tcagcgatgg cgcttattgc tacaagtatt gaacgttttg caacagagat acgcggattg
     4981 caaaaatccg aaacacgtga agtagaagaa ttttttgcga aaggtcaaaa aggttcttct
     5041 gctatgccgc acaaaaggaa tccgatcggt tcagaaaata tggctggtct agccagagtg
     5101 gtgcgcggac acatggtcac tgcctatgaa aatgtcacat tatggcatga aagagatatt
     5161 tctcattctt cagcagaacg aatcatcttg cctgatacca cgattttatt gaattacatg
     5221 ttaaatcgtt ttggcaatat cgttaaaaat ctaacggtct ttcctgaaaa catgaagcgg
     5281 aatatgtctg caacatttgg tttgatttat agccagcgcg ttatgctgaa attgatcgat
     5341 aagggaatga caagagaaga agcttatgat cttgttcagc caaaaactgc gtacgcttgg
     5401 gacaaccaag tgcagttccg cccgctgtta gaagaagatc caaccatcac atcggtatta
     5461 tctaaagaag aaatagacga tgcctttgat tatcatcatc atttgaaaaa cgttgacgtg
     5521 atattcaaac gagtaggttt ggaataaaga agagtgagtt gcaaaattca attaaaatag
     5581 aatagcaaaa a
//
LOCUS       DAZKLA010000148         5481 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000148 DAZKLA010000000
VERSION     DAZKLA010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.151"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ACG85N_002565"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ACG85N_002565"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="ACG85N_002566"
     CDS             complement(580..1353)
                     /locus_tag="ACG85N_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0225278.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="ACG85N_002567"
     CDS             1497..1847
                     /locus_tag="ACG85N_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0225279.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="ACG85N_002568"
     CDS             1828..2544
                     /locus_tag="ACG85N_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFQ0225280.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="ACG85N_002569"
     CDS             2544..3992
                     /locus_tag="ACG85N_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0225281.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="ACG85N_002570"
     CDS             complement(4062..4571)
                     /locus_tag="ACG85N_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225282.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="ACG85N_002571"
     CDS             complement(4561..5286)
                     /locus_tag="ACG85N_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225283.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1864 a          955 c         1095 g         1567 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt t
//
LOCUS       DAZKLA010000149         5429 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000149 DAZKLA010000000
VERSION     DAZKLA010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.152"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            84..2006
                     /locus_tag="ACG85N_002572"
     CDS             84..2006
                     /locus_tag="ACG85N_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0225284.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            2007..2453
                     /locus_tag="ACG85N_002573"
     CDS             2007..2453
                     /locus_tag="ACG85N_002573"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFQ0225285.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            2469..2783
                     /locus_tag="ACG85N_002574"
     CDS             2469..2783
                     /locus_tag="ACG85N_002574"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFQ0225286.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            2809..3858
                     /locus_tag="ACG85N_002575"
     CDS             2809..3858
                     /locus_tag="ACG85N_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0225287.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            3870..>5429
                     /locus_tag="ACG85N_002576"
     CDS             3870..>5429
                     /locus_tag="ACG85N_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0225288.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSA"
BASE COUNT         1962 a          767 c         1085 g         1615 t
ORIGIN      
        1 ttctacatta atcaagaact ttcttctgta tgaaaataga gataataatt gtacacttaa
       61 gctgtattca ggaggtgaag ataatgggca tcgaatttag cgaacggcaa atgaaaatac
      121 tatacttttt gattgaaaat gagcacacaa cttacaaaga attatcagaa caattatatg
      181 tatctacgaa aacaattagc aaagatattt taattttgaa agaaataatc caaaaaatgc
      241 cagatgtaca aatgttggtt caacctaaaa aaggcgtttc gcttattgga aaaaaagaaa
      301 cacttcgaaa tttattatcc agtcataaaa tcaatatcgc agactctccg gaggaccgtc
      361 taacttatgt gtttgctaaa ttattgaata ctaatgattc tctaaagatt caaaatcttg
      421 ctgacgaatt atacgtgagc aggtcgacgg tagaaagcac aatgaaagat gttagaaaaa
      481 gactaaacaa gcatgggatg tccttagttt cagatcgaaa tggcgtgcgc atcaaagctg
      541 atgaaaaaag taaaagaagg ttgatgtcag aagttgtgaa ctattattgg ggtggcttta
      601 cagcatctaa cagtaaaaca gatctcttag acttgaatat caatttcatt ggtgaagcga
      661 ataatttctt aaataaggac atattgaaaa aagtagccga attgcttaat cgatacatga
      721 ataagacaca attgaaattt acagattatg aatttcaaac tttagctatc caccttgcta
      781 ttgcgataga aaggattgaa acaagcaatt tcttatcacg tagtaataat tctgaagaag
      841 aaatctcaaa gaatacactt attttaatca aaatgattga aaaagcattt tcaattattg
      901 tgccaataga tgaacaagct tatgttgaca ttcacattac ttcgattgaa aaaaatgcta
      961 tgaatcacga cttagatcaa acgaagcttt cctatttaga tatgggtgtt gagctacaag
     1021 agttagtaag aattaaatta aatgaatttt atcctgatga agaattgatc aataatctag
     1081 tattacatct caatgcagca gttaaaagac tcaaattggg agtaaatatc tataaccctt
     1141 atacggacaa aatcaaatat tcttttaaaa ggtcatttat gataagtgta gatttattag
     1201 aggaaatcga agagagattc tgtatacatt ttaatgaaga tgaaatcgct tacgtaacac
     1261 tccatattca atcacttttg gatcgttaca aacctgacaa aacgaaagtg atattagttt
     1321 gtagtagcgg atatggaaca tcaaaattat tagagcaaag aataaataac ggttttgcag
     1381 atatggttga gatagaagat gtactaagca tcaatgaact acaagataga aaagtcactg
     1441 atgaacttgt tataagcacg attccgattg aaaatacaaa ttttcctgtt attgttgtta
     1501 gtcctttgat gttaggggca gatgtcagaa aagtaaaaca gtatctacag ttaaatgaac
     1561 aaacagaaga tgcttttcta aatttattag ataaacgatt ttgctttttt acccaccaaa
     1621 taaaggataa tcaagtgaac gttttaaata taattaccaa aaaattggta aaagaaaatt
     1681 acgcgaaaga agggatacta gagggagcaa tcaaaagaga aaaactatct tcaacagcga
     1741 tgaatgattt tgctatgccg catatcgaag tagattttat aaaaaagcca gtaatctgtg
     1801 tctacgttaa tccagaagga attgattggg atgggacact tgttcattct gttttctttt
     1861 ttgcacttaa tgaaaaagta aaacctgaat tgaataaaat ttatgaaaca ttcaatgaaa
     1921 tcgcttcgaa tcatgagtta ttagcaaatt taaaaacgag ttcgtcgttt gaaaaattag
     1981 tttatatatt gaaagaaggc ttataaatga tcagtgaaaa aatcaatata aataatatag
     2041 tgatagattt aaatgcatct gataaaaaaa gtgtagtcga aaaattatcg aaattgttag
     2101 agactaatgg gtttgtgtct gaccacaaac aatttcaaaa agacgtattt gacagagaaa
     2161 accatatgac cacaggaatt gggaaaaata tcgccattcc tcatggcaaa agcctagcag
     2221 taattgaatc aacagtggct gtagccaagt taaagcggtc tgttgaatgg ggcgcattag
     2281 atgatcaacc agttgatctc gtctttttgt tagcaataaa agacactgat aaaggtacta
     2341 atcatttacg tattttagca gaattatctg gaagattgat ggatgacgag tttgttaaaa
     2401 gaataaaaga ttcgagaaat aaaaaagaat tggtaaaagc tctatcattt taaaaggagg
     2461 aatttcatat gacaaaaata gtagcagtaa cagcatgtat cgcagggatt gcccatactt
     2521 atatggcaaa agctaattta gaaaagtttg cgaagaagaa agggttagat attaaagtag
     2581 aaacacaggg ggctatgggg atagaaaaca aactttcaat ttcggatgtt tccgaggcag
     2641 atgttgtcat atttgcagta gatacaaatg tttcagaacg aggtcgcttt gacggtaaac
     2701 cgattttaac agtagggacc tcagaggtag taaaagatgg tgaagagacg attgatgaag
     2761 caattgcatt aattaacaaa taaaaaatat atcgtggggg agttactaat ggaattcaag
     2821 aaacaaggaa gagaaattct aaatcatttc caaagtgggg tttcctatat gatcccgctt
     2881 gtcgtagcag cagggttact aacttctatt gcagtcattt ttggtggcac aggtgtttgg
     2941 gaccagacgg atacattctg gggagtcctg cgaatgattg gtcagacggg attacagttt
     3001 atcgtaccga tgatttcagc ctatatcgca tattcaattg ctgaccgtcc cggtctagca
     3061 ccagctttta tcactgggat gatgtgtcaa aatcttggta tgggatttat tggaggaatg
     3121 gttgctggat tagcttgtgg ctatttagct aatgcattga aaaaagtaaa agtaccgatg
     3181 caagttatta gtctaaaatc aattatgctg attccttttg ttactacatt agttgttgga
     3241 atcatattat ggtatgtact agctacgcct attcaagcaa tgacaacagg actgactaat
     3301 tggctagaag gcatgtcagg agcaaatgct gctttgatgt cggctatttt aggaggaatg
     3361 atggcgtttg atatgggagg acctatcaat aaaatcgcat atgcttttgg ggttgcttct
     3421 ttttcaagcg gaagctttgg acctagtaca gccatgttat taggaattgc tattccgcca
     3481 tttggaatgt ttttagcaac aattttaaac aaaaaacttt atgatgaatc ggaacgagat
     3541 aatgcaaaga cagctatgat tatgggcctt gtggggatta ctgaagggac gatcccgttt
     3601 gccgtgaaag atcctttacg agtaatacca agcatcgtgg tgggcactgc agtagcatgt
     3661 ggtctaaatg gattttttgg tgttactcaa gaaacaatgc tttcaacctt tatggcaatt
     3721 ccttttgtta cgaatatacc tttatattta gtatcaattg ttattggggg agtggttaca
     3781 gcgttaatgg tcaatgcact aaaatccatc aagtataaaa aagaacaaca agtaaattca
     3841 gtagaagaag gaaagtagga gaatctgata tggtaaatgc acatataatt caacacacgc
     3901 actgggacag agaatggtat tttacaaatg aagatgccat cgtattgtct gatcaggtct
     3961 ttactgaagt tctagatgaa ttggaaagga accctaaagt aaacttttgt ttagatggac
     4021 aaacttcaat cgtcgatgag tatttggaga tcaatccgca aaagcttaag gttatccaac
     4081 gactgatgaa aacaggacaa ctattcgtgg gaccgtggta tgcacaaaca gatgccttat
     4141 tagtggatgc tgaatcaatt ttaagaaact taatgattgg aatcagtgat acgatcctta
     4201 aatatggaga tccaatgatg gtagggtatt tgcctgacac gtttgggttc aatgcgaatt
     4261 tgcctacact tttgcatcaa gtagaaatcg ataatttcat gtgttggcga ggattaaact
     4321 ttgaaacgat ggttccatct ccttatttta tctggaaagg attgggagat aaatttgttt
     4381 atgccatgaa ttttccattt ggttatatga ctggaatgat gaccttagac gcaattaaag
     4441 atattcctga atttgcaaaa gaaaagttgg atgaagcagt tgaaatgctt catatgcaag
     4501 gaaaaaacaa agatatttta gtaccttcgg gtgtagatca aaagtcaatg attttaaatt
     4561 ttgatcaagt cgtagagaag ttgaataaat acagtaaatt ccacaatatt atttcagatt
     4621 atccttctta tgtagaagtt atcagaaaca aaaaagaatt acctgaatat aaaggtgaac
     4681 ttcgtcagcc ggtttactct cgtgttcatc gttctattgg ctcagtaaga acaaaaatga
     4741 aaatagaaaa ttttaaattg gaacaaaaaa ttctaaaacg agttgagccg ctcatgctga
     4801 ttgcagaaag aaatggtatc catatcagta aaggcctact tcgtcggtta tggaaaaaga
     4861 ttttgcaaaa ccaggctcat gatagtattg gtggctgtgt ttcagataat gttgcggaag
     4921 atattttcca tcgaatcaaa gaagctaatg agatagctga cggaatcgaa aaccttattg
     4981 taaaacggat ggctgatgca ttaaagctaa aatcgaatga agtccttgtt ttcaacacaa
     5041 cgactgagaa ttatagtgga tcgaaaatcg tgcatgtagt agctgattca aagaaaatac
     5101 acttcaaagg tagtccctat aatatcattg aaaaagaagt ttattttcca gaacggaaaa
     5161 atgcttatcg gatgattgct acaggtttcg aatattttga tgaaccagct tattatgaac
     5221 ttgacataaa aataaatgta aatgtgccag cactaggata cactgttgtt gaatttgaga
     5281 aaaataatga aaaattagaa acagcaatcg agatagacaa aactgagata agtaacaata
     5341 aatataaact ttcttttaaa gatgggcaat tgaatttgat agttggagat agacaatatc
     5401 ttgatttcgt tcatttgatc gattcagca
//
LOCUS       DAZKLA010000150         5391 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000150 DAZKLA010000000
VERSION     DAZKLA010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.153"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..184
                     /locus_tag="ACG85N_002577"
     CDS             <1..184
                     /locus_tag="ACG85N_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225289.1"
                     /translation="KKTMLVLGSISLGVLGFSPMLLSVIGKNNSKGELKLNPNYYNSP
                     NEPMFISEKQNDGQDK"
     gene            complement(203..565)
                     /locus_tag="ACG85N_002578"
     CDS             complement(203..565)
                     /locus_tag="ACG85N_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225290.1"
                     /translation="MKKTMLKVFVGLFLLTGIGGIFSKTVDAGSYYYDKSSGNYVINV
                     TNSETGKNLNSIAGKTPGSVTNKMRNAAIKRYGSIQNNRRYYFSYKNIVTPYGGKKGG
                     VSFQWTGRVINMNCTGRS"
     gene            661..1032
                     /locus_tag="ACG85N_002579"
     CDS             661..1032
                     /locus_tag="ACG85N_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0225291.1"
                     /translation="MNKNKYYPIYEDLKYKIQHGEYKENEKIPSENQLCNLYSSSRAT
                     VRKAIELLAIFGFVDRSQGKQTIVSSKKNWNKRFLTEEEYKKLVLFNLKSTELNEIIA
                     LSEKYSVLKKLVDFWNNNNLI"
     gene            2138..3070
                     /locus_tag="ACG85N_002580"
     CDS             2138..3070
                     /locus_tag="ACG85N_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0225292.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFSQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEVDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKHEDLRIKLFNIEHNGELTQEEKWRAKDRILNLPLGTHEAD
                     FKKQQQTEKEGTQKQALSNELKQDLIGNLQNLFD"
     gene            3086..3610
                     /locus_tag="ACG85N_002581"
     CDS             3086..3610
                     /locus_tag="ACG85N_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0225293.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSKENRKLWFSKVGNKFVIND
                     IGQKAIKSMFYSENENLNRKLVSGKTKTENLKNANQVSELELVSLLNKQLDQKDKQLQ
                     EKDTQLKQMQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKTEKKWWQFWQ"
     gene            3986..4267
                     /locus_tag="ACG85N_002582"
     CDS             3986..4267
                     /locus_tag="ACG85N_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225294.1"
                     /translation="MDILVRKIDSKIISEIDRRCQELTTKTSKKWSRNTYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNEYVRTNNQLIETLIGGL"
     gene            4269..4685
                     /locus_tag="ACG85N_002583"
     CDS             4269..4685
                     /locus_tag="ACG85N_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225295.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISILSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNHQKETNQTFTAGNFDPTNFLKR"
BASE COUNT         2018 a          871 c          867 g         1635 t
ORIGIN      
        1 aaaaaaaact atgttggtac taggttctat ttctctagga gtattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaataattc caaaggggaa cttaaattaa atccaaatta
      121 ctacaatagt cctaatgaac ctatgtttat ttcagaaaaa caaaacgacg gtcaggacaa
      181 ataatcctgc cgtcgttttg ctctaacttc taccagtaca attcatgttg attactcttc
      241 cagtccattg gaaacttacg ccaccctttt tacctccata tggcgtaact atgtttttat
      301 aagaaaaata ataacgtcta ttattttgaa ttgaaccgta tcttttaata gcagcatttc
      361 tcattttatt agtcactgat cctggagtct ttccagcaat agaatttaaa ttttttccag
      421 tttcagaatt tgtaacgtta ataacataat tcccactaga tttatcataa taataagaac
      481 cagcatcaac tgtttttgag aaaataccac ctataccagt taatagaaat agaccaacaa
      541 atacttttaa catagttttt ttcatataaa aacctccttt agtttaagaa tatactaata
      601 attataaaga aaacgtttta tattatcaat gttaaaataa taaaaaggag agcttgatct
      661 atgaacaaaa ataaatatta tcctatttat gaagatttaa aatataaaat tcagcatgga
      721 gaatataaag aaaatgaaaa aattcctagt gaaaatcaat tatgtaatct ctactctagt
      781 agtagagcaa ctgtcagaaa agctatagaa ctattagcta tattcggatt tgtggataga
      841 tctcagggaa agcaaaccat tgtttcatct aaaaaaaact ggaataaaag atttttaacc
      901 gaagaagaat ataaaaaatt agttctattc aatttaaaaa gtactgaatt aaacgagatc
      961 attgctttat ctgaaaaata ttctgtatta aaaaaacttg ttgatttttg gaataacaat
     1021 aatttaattt aactagcaaa ttttctaaaa agataaaaaa agaagtgggc aatatgggaa
     1081 attaaaccac tacttttttg ttttgttgct ccagcaacgc ttagtcctaa attatccgaa
     1141 agataaaata acaaataaaa tgttcattta ccatataaat ggtcgctaaa ataagcccac
     1201 aaaaaagagc cattaaccct ttgattataa aggattaatg gttcttttat tcttcgaata
     1261 ttttctattt atttgataaa atcacgattt ttttgaattg tgctaccaaa ataaatgcct
     1321 gtatatcaat cattgctacc aaatatttct ctgttattta gcattacgct cccctattag
     1381 agtagggggc acagtatttt ctacctgttt tgggggtttg tcccacatag cattggtgtt
     1441 caagtgctcc gtttttctgt caaaaaggtt cttcctggtt tcatatgttt atgggcctta
     1501 atttgcattt taagttgttt tggtgtctga atcatataag tataccaaag gatgggcaaa
     1561 acagcttaaa tcgaatttta gaaagggttt atgacgttcc agggttgtta gggcttgccc
     1621 tgaccgccta taaggcgctt taagagcaag ataaggattt ttatttaact tgtcttagaa
     1681 agccttaaac aagcatttag ggaataataa aggccttttt aggaacgggt tgaagcaaag
     1741 gagcctttag tcgactgcgc agtacaatgt gagagcgtag cgatcacacc tttttagcga
     1801 ccgtagggag ccagtttccc agtttaggaa aatgcgcgaa tctgtttgag gagaaaagga
     1861 gtaatttttt aggcgggaaa ttttgccctc cctcttttat aatctctttt taaatctctt
     1921 tttaaacctc tttaccgtcg tctgttccct tactctccca aggggtgtag cttttttagg
     1981 gcatgaaatt atctgtttat aggcatgaaa ttatctgttt ataggcatga aattatctgt
     2041 ttataggcat gaaattatct gttttaggta ttgttttaag acataatatg tcttaaaatt
     2101 atgtcaataa aattagtgag actggagtat caaataaatg gcaaatgaaa ttgtaaaata
     2161 tgaaccagaa ttaaatacca ttcctttaag aaaatttacc ccaatagaaa tgaacttgtt
     2221 tttttctatt gtctctagaa tgcgtgataa aggtaatcaa aaaatacgct tttatttcag
     2281 tcagcttaaa gatttaagtg cttataagca aactgctggg gaagtcttta taaaagattt
     2341 aacaagaaca tacgatcatt taatggattt acgttttgga aaaaaaagca atggtggatt
     2401 aaccatagaa cgatttacaa tgtttactga ttttgaaatt catggagaag tcgataaccc
     2461 ttatgtagat ataaaagttc acgaaaaagc tttgccttta cttaacaatt tagaaagttg
     2521 ggtacgtttc tctttaacag aatttcgaga tttaaatagc agttatgcta aaacaatgtt
     2581 tagattgcta aaacaatacc gaactcaagg atgggctgaa ttttccaagg aagatttttt
     2641 agagctatta gatattccta aaagctatag acaaacaaac ataaatcagt ttgtactcaa
     2701 acccataaaa gaagaactaa ctccactgtt taaaggatta acgattcgta aaaaatatgg
     2761 taaaggacga ggaaagcctg ttattggtta tcggtttact tggaaagctg aaattaatca
     2821 tgctgacgat ttctcaaaag gaaaacatga agatttacgt ataaaattat tcaatattga
     2881 gcacaatgga gaattaactc aagaagaaaa atggcgcgct aaagatcgaa tattaaatct
     2941 tcccttaggc actcatgaag cagattttaa aaagcagcaa caaactgaaa aagaaggaac
     3001 acaaaaacaa gcactctcaa acgaactcaa acaagattta ataggaaatt tacaaaactt
     3061 attcgattaa atataaggag tatttatggc agataaaact atcaaagaac ttgctgaaga
     3121 attaggggtt agtaaaacag caatcaataa aaaagtttct aaagaaaatc gaaaactttg
     3181 gttttcaaaa gtcggaaaca agtttgtgat taacgatatc ggtcagaaag ctattaaatc
     3241 aatgttttat tccgaaaacg aaaacttaaa tcgaaaacta gttagcggaa aaacaaaaac
     3301 cgaaaacttg aaaaacgcta accaagtttc cgaattagaa cttgtatctt tattgaataa
     3361 gcaattagac cagaaagaca aacagttaca agaaaaagat actcaactaa aacaaatgca
     3421 aaaattactc gatcagcaac aacaactcac cctgcaagcc aaccaacaaa tcgagaaact
     3481 gcaggaacaa ctacagttga cttatacgga agaatcttcg gaaaataaat caactactct
     3541 ttctgaagaa acagaatcga tagaaaaaca atcaaagacg gagaaaaaat ggtggcaatt
     3601 ctggcaataa aaccagcaat ttttattaat aaaaattgct ggttttattc ttctttttgc
     3661 ttttgcgagc agagcaacat gggcccatgt tgcgatattt tggtagcgcc ccaatccccg
     3721 ctttttcgtt gaatcttact cagactgcac tcgttttata gctgattttt ctgaattttg
     3781 aaagagcata aggaaacgtg gtatcacttt cttacgaaag gtgatagata ggctgtgcct
     3841 atcacatttc taagaaaaaa atttgctatc acttttttgc cattatggat agtgtatgta
     3901 tagtattttg gtaaaaaaca gctatacaaa tactatattt gtatgatata catagattat
     3961 acgcacatgc gaaaggaaaa ttgctatgga tattttagtt cggaagattg attcaaaaat
     4021 tatttcagaa atagatagac gttgccaaga acttacaacc aaaacgagca aaaaatggag
     4081 ccgtaatacg tacctaaaag ttctaatcga aaatgatttt gaccaggcac ttaggcatta
     4141 caaaaaagat caattcgatt tattactcga aaaatttatc gatatacaag caaaaaatac
     4201 ggaaacactg aatgaatatg ttcgtacaaa caaccaactc attgaaacac ttatcggagg
     4261 actataacat gagaaaaact tctgttcaaa aacacgttac cctttatccc agtcacgtgg
     4321 acgttttaga ttccattatt gaaagaaatc cagatatcaa caattactcg gaagccattc
     4381 gttttttatg tatgagttat gaacctgata ccaacacaac cactactagc aaccaagcca
     4441 aactaaatgc tatgggaaaa gaaatttcta tcctgtccga aatcattgca gaatttgctg
     4501 atttatatct aaaagatact ggtgttctta ttggtaccaa ttctaaggtg tatcaagaag
     4561 tgaaagaaaa aattgaaaat caactcaatc cttcccgacg aaatcaaccc aaaaatcatc
     4621 aaaaagaaac caatcaaaca ttcactgctg gaaattttga cccaaccaac tttctaaaaa
     4681 gataaaaaca gaacctgaaa aataaaaaaa ttcctcgatt tctttttagg gggggcggtc
     4741 ttccagcaag atttgcaagt tgggaccaac ttgcggaaaa aaagaaaaat tcgcttgcgc
     4801 gaatccttct ttttgcattt tggggcgctg ccccaatccc caagtatcgg ttatttaata
     4861 gcgtggcaac tcaaagaaca gaacatactc tgaaacaaaa aaagactgct ctaacgagca
     4921 gccagttaaa aaataatgag tgtttattct tgaaaaagca acgcgcttat gaagcgctac
     4981 caaaaaatta tcatatttta ttaaaaataa caacccagta aaaaatgact ggtaaaaatt
     5041 cacaaagaaa cggaaagaag caaccgcaga aaatcaagct taaaaagagc tttttaccta
     5101 gaccaatgaa aaatcacaaa gtccgcccct gtacctgttg gtagctgggc tacttggcat
     5161 actgtacatt atgttaactt gaatttaaaa taacatgtat tattgtatta taaagcggac
     5221 tctattgaaa tctataattt tcacttttaa atattacaaa aaatcatata atttgagtta
     5281 ttattgttta gaagtgattt ttacaattta aacatttgac aaactataga acagcactta
     5341 aaatgtgtat acaagtgaca gcgctttaaa aaaggaggtg cagcctatgt t
//
LOCUS       DAZKLA010000151         5295 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000151 DAZKLA010000000
VERSION     DAZKLA010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.154"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..370
                     /locus_tag="ACG85N_002584"
                     /pseudo
     CDS             21..370
                     /locus_tag="ACG85N_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(642..932)
                     /locus_tag="ACG85N_002585"
     CDS             complement(642..932)
                     /locus_tag="ACG85N_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225296.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1334..1570
                     /locus_tag="ACG85N_002586"
     CDS             1334..1570
                     /locus_tag="ACG85N_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225297.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1577..3529
                     /locus_tag="ACG85N_002587"
     CDS             1577..3529
                     /locus_tag="ACG85N_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0225298.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3601..4098)
                     /gene="dfrG"
                     /locus_tag="ACG85N_002588"
     CDS             complement(3601..4098)
                     /gene="dfrG"
                     /locus_tag="ACG85N_002588"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0225299.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4425..5117)
                     /locus_tag="ACG85N_002589"
     CDS             complement(4425..5117)
                     /locus_tag="ACG85N_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0225300.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1717 a          916 c          869 g         1793 t
ORIGIN      
        1 gttatctttt aaatacttaa ttgttaaata ccttatttga tgcaaaagaa atctacgata
       61 gtcaacaaaa aaattttcat tatatccaga aaaatttttc ttagttaagg acctatggat
      121 tgcagtaatg cagaattcgt cgaataaact gccaaagaca tacaatcata ttgcttttaa
      181 aattgacgaa caagatatag attcgtttgt ctcaaaaatc caaatgttag gtttaactgt
      241 cgaaccagga cgttctcgtg tgaaaggcga ggcttcttca atttactttt acgactatga
      301 taatcacctc ttcgagttac ataccggtac tctccaggaa cgtttaaaat cctacaattc
      361 cactacctga gactagtaat gactagtctc tttttatggc ctctgatccc cccattgagc
      421 gaatactaat agggcttcga attgatctgt tcaatcttta agcaatatcc ctcaaatgag
      481 tttggaaagt ttagtgattc atcacaaata cttaaataca tgtagttata tatagattga
      541 ggttcttgtg agcgtatgct cgtgtgggtt caaatcccat cgtctgcatc catctttcca
      601 aaacaagcgg ctaggacatt tttgtctcag ccgcttgttt tctagtttat tctttcatct
      661 tgtcggacct tttttcttgg gtatagactt ttctcatatt ctaccacaat tttgggtttc
      721 cctaacacat gataggatct cacaacaaat tgctttctat tattataata acctgtaacg
      781 actaacttca tcccttcgtc tacttcagct aggaagttca aactatggga agcaatcaaa
      841 caattgaccc catttaaact gaaacggatc aacggtctgg cactaacttg caaagtacgg
      901 atcttatcta ctgttccata ttttttctcc ataaccttca cctcaatacc attatacgaa
      961 catacattcc ctttgtaaag aagaaactct gttttatctt tttgactttt tgtctattac
     1021 ctttcttaaa atcatattgg cacttcctgc gaagatgaag gacatgtact aaaaaaaaga
     1081 ggcagcagta tagatttaag tagttcagtt ttggagtaca aaaagtgaaa atgtaaacct
     1141 aaatgagtat caaattgcaa ctgaatttga tactcgtttt ttattgttaa atcaacgttt
     1201 gttgttaatt tacaccacaa aatgtaagtt aaaatgtata taaaaatgcg aacgtgaaat
     1261 aattattgta ggaatgcaaa ttggtgtgtt aaaatggtta caatttacta agcaaaaagg
     1321 ggtgcatttt cctatgcaat ttcttgagcc aaaaaataag aacgctaagt ctgtggattg
     1381 ggaaatatca gagcaagtaa gggtgatagt taagcaatac gctgaatatg cagagcgtac
     1441 tgaaagcgaa gcagtggatg aattcttatt gaacatttta gacgataaga agtttattga
     1501 atggattgcc aacaagcgaa gtaataagcg gattgttgaa aaaatgggta tcaaagatag
     1561 agtgggttga tagacaatga ccaagcttaa aagattagcc acgaaggaag atgaaattgt
     1621 agatgttaaa attccgattt caaacgaaga attaaaagat agggctaaac aatatgattt
     1681 attaactcca aaacgatttg caacaaggta taacaaaatg ttgttcttgc caacgtcttt
     1741 taaatggaac ggttctgaat atcccattca atataattac tgcatcaatc cgttttgttg
     1801 taattttggg aaagaacagc acaaattcaa agatgtaaaa gggaaaccaa gtcgatataa
     1861 aatgactggt agttccaaag ataaaggtca taaaggtatg tactgcaatg acaatcctat
     1921 tggcagaggt gtatcgcaaa actgtacagt aaccccttta tctaattggt ctgttgttga
     1981 agaaattaaa cgccttatag aaatcaatag catccaagat gttgagccag attatcaatt
     2041 tcataaagaa ggctgctcgg aagaagaatc aaccccattc aatgagccga agcaatttta
     2101 caaaaggggg aaaagtaggg gcaaatctca acgctatcaa tgtaaggcgt gtaagaagtt
     2161 cactaatgtt ctaccaaaaa gagaagaaac cactacttat caccaacaaa agaacaccat
     2221 tcttccaatg ttcgctaaga tggttgttgg aagggtttca gtcagtcgta cttgtgatat
     2281 tttaggaatt ggagttggaa cctactatca taagctggag tggctgtata ggcgttgttt
     2341 ggagttttta gaacgatatg agacacaacc ccttcaaaca aagaaattta acgagatgtg
     2401 gcttaatacg gacaagatgc actactacct aaataacgtc cgtaagaaag gacaaggttc
     2461 aaagaaatat acggggtttg aagaccttaa tatgcaaacg tatatagttg tatctgctga
     2521 ggtcttatca agatatgtgt tccgttctga tgttgcctat gattggaata tttcaatgga
     2581 tgaattaaat gaagatacaa gaaaattcaa agaagaccat ctcaatactt ttagtcgtaa
     2641 aaatgatagg ttagattggt cttattatcc acaagaacca tccgctaacg atagcgaaaa
     2701 taggaatgct tatcttcacg aattagggaa aattactaat aggagtagat ttgtcgatgg
     2761 tttaaatgtt gatgctcctt atactacaac tgctcactat tggttaatta aacaaatggt
     2821 taatgccgac gaatggcgaa tgattagtga tgatgacttc tctattagaa acgcctttta
     2881 tagagtattc acgaaagaat taaggttgag cgatgcacat cattttattt gccaagttaa
     2941 caagacgaaa agcagaaaac aatgcttaaa agaattcgga caagcaaaag cagaactgct
     3001 cgattgggga gatatcagag gattcaaaac aaagtttcta cggactttgg catctcatta
     3061 tctaactgaa ttactaacaa gtcatcaatt ccacgaagag gctattagta aagatggaga
     3121 aagatatcgc aaatatgccg ataaccctat caaacatcca ttagccacta aggataaagg
     3181 attttattca gttgattgta gaacggattt atctgctctg gaaccaaacg aaatcgcaaa
     3241 gatgctatta aatgttaatg accattcgac caatagcttt attcaacaga taagacgtta
     3301 tatatcatct cttgaaagac cattaacaac agctcgtggt gataagaaga gctatattta
     3361 cgctaacttc aatccgaagt atgctcaatt tgcgattaca attctaagaa cttactacaa
     3421 cttttgtcgc ccttttaaat cagcggataa aaaggttttg acacctgctc aaagattagg
     3481 gattacagat aaacaatttg attggaaaga tataatttat ttcaaatgac aaaaagagct
     3541 tggagtaatc ccaagctctt gtgctttatc tttactgttc ttcttcaatt aacgcacccg
     3601 ttaactcaat aagttttttc tttcatatac atgaaaatag tagttatacg gatttttatc
     3661 attctttatc cctttttggg caaatacctc attccattcc tcataattca cttctggaaa
     3721 aaaagtatct ccttcgaatt catgatgtat ttttgtgatg tacattttct caacataagg
     3781 gaaaaacaaa ttataaatct gttctcctcc gaaaataaaa atttcttctt cgtttttaca
     3841 taactcaaaa acatcttcta ttgaatgaac aatttcacaa ccattaaagg taaacccctt
     3901 atctctcgtc agaataatat ttcttctgtc aggtaaggct cttccgattg attcaaggtt
     3961 cttcctacct aatattatcg gatgtccctt tgtagtattt ttaacatatt cccagtcctt
     4021 gggaatcctc caaggaatgt cattctcttt gccaatcact ctattcttat ccatcgcagc
     4081 aatcaaagaa actttcattt ataacacagc ctttttaact aatttaccat ctaatttctt
     4141 atttctatca aaaaaccttt tctcattatc ctgcccgtta gttgaataag caattttttc
     4201 tataagttat attatcataa taccacaaaa tcacaaattt tcattcaatt atcattttca
     4261 attgcatttt ataaatcatt ttgtatgtaa tttggtatgt aagttaaaat gaaaaaagta
     4321 gtccattttg aagtacattt tgtcattttg tactccaaaa ctgaactact tagtatagat
     4381 tactgttgct tctttactaa tttaggttta cttgttaaga aagattattt acgcaaatgt
     4441 tcttcaacaa tttgctgaga tttatcagct gtatcttcca cttcatcttt tgctttttct
     4501 aacttttgaa tatattgttt caagcttgag ttttcagcag ttgcttcgtc taatttcttt
     4561 tgtgtatctg ttaactgagt ttctacattt gttttttctt gtgtcagtgt tgcgatttgt
     4621 tgattcaaac catttacagt attttgtaat tctgaaagtt ttgcttgtgc ttcttgtagt
     4681 cttgctgtcc aatctgcatt cagatgattg atttcatcat ttttttggtt gatctcatct
     4741 agtttgacat tgatttcatt ttgtttgctt tgtagttgtg catccttgct gactaattga
     4801 ctttctaagt tttgtttttc tgctgccgat tgattttttt ggttttctaa ttcatttact
     4861 tttgtttggt attcttggac atctgccttt gaatcttgca atgtctgttc tttttgtgct
     4921 agtcgagcgg taagatctgt aatcgcttgc tctttatctg tcaatgtatg atcaagctgt
     4981 gtcagattct cattgattgt atcaatatct gtctctccac cccacaaaat aatttcattt
     5041 gcgtaccgcg tgcctgtgta gccaattaga catagaaatg caacgacaat cccaccagta
     5101 ataaatttct ttttcatttg catagctcct taatccaata cttttacatg ctaaaagtaa
     5161 gtgatctcaa acacaatgtc aagcgttttt aaattacata tgtaatttta atataaatac
     5221 tatcaaatac tcatttaatc ctcctttttt atctatttaa aaaatctttt ttatagagaa
     5281 aaatgcaggt taata
//
LOCUS       DAZKLA010000152         5221 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000152 DAZKLA010000000
VERSION     DAZKLA010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.155"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..366)
                     /locus_tag="ACG85N_002590"
     CDS             complement(46..366)
                     /locus_tag="ACG85N_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0225301.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(514..2748)
                     /locus_tag="ACG85N_002591"
     CDS             complement(514..2748)
                     /locus_tag="ACG85N_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0225302.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3227..3493
                     /locus_tag="ACG85N_002592"
     CDS             3227..3493
                     /locus_tag="ACG85N_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFQ0225303.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3493..5220
                     /gene="ptsP"
                     /locus_tag="ACG85N_002593"
     CDS             3493..5220
                     /gene="ptsP"
                     /locus_tag="ACG85N_002593"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFQ0225304.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
BASE COUNT         1568 a          978 c         1049 g         1626 t
ORIGIN      
        1 tttacttttg tcccacgctc tttaattatt atgttcaaaa tttgtttaca cggctaatgt
       61 agcgcctaat tcatgttttg gaatcgagat ttcaacaagg cctgccaatt cgattacttt
      121 gatttcatcc tcgtggtaaa cggcgtcttt gattttatct aagaaataat ttttagcatc
      181 ttcgatttct ttcggctgga ttttacgttt gtcattgatc agcagaattt cattatgttg
      241 aggggcttca gtgtagacaa tagtggtatt tccggctgtg tagaccttga ctagatgggc
      301 atcagtgttt tcaagctggt ttaatacaag acgggaatgg ctgtttgtta catttaccaa
      361 tttcatggcg ctcacctctt ttatgtattt ttaattgctt gtttctctta ttataatttc
      421 ttttttataa aatgaacagt attttgactt ttctgaaaaa agaatgagac ggcatttctc
      481 aaagaaatac cgtctcacgc tataaaactg gatttattca gccgtttctt cggctgattc
      541 tttagccaaa cgttctggtt tagaagtaac gatgattttt ccatctttat ccagttttgc
      601 tttcaactca tggatagaag ggtgatcaag ataaaattca gcgataccat cttcgatttg
      661 ttcttggatc gtgcgtcgaa gaggacgagc acccatagat ggatcatagc ctaagtcaac
      721 taatttttct ttgacattcg taggaacttc gatgtgaagt tgctgtgctg ccagcatttg
      781 gttgacatcg tctagcatta gtgtgacgat tgtcatcaag ttttcttttg ataatgcact
      841 gaattcgatg atcccgtcaa aacggttcag aaattctggt gtaaagtagt tgtttaattg
      901 gttcaataca gaacgagtaa caccttcgcg tgccgcacca aaaccaacgt ttgcttctac
      961 tttaccagtt ccggcattac ttgtcataat gatgatcgta tctttaaagc tgaccgttct
     1021 tccttgtgca tctgtcaatc gaccatcatc taagatttgc aagaacatat gcaaaacatc
     1081 tggatgagct ttttctactt catctaacaa gacaagactg taaggatttc tccgtacttt
     1141 ttctgttaat tgaccagctt cttcgtatcc gacataacct ggaggagaac caatcaattt
     1201 agagacacta tgtttttcca tatattcaga catatcgaaa cgaatcattg aatcttgtga
     1261 accgaatagt tcgtaggcaa gttgtttcgc aagctctgtt ttacctacac cagttggtcc
     1321 aacaaataag aaggagccga ttggacgatt ctgtttgttt aatccaacac ggtttcggcg
     1381 aatcgctttc gctactcgat ctacagcgtt atcttgtccg atgacatgag ctttcaagtc
     1441 atctgctaag tttttcagtt gtgtctgttc tttttctttt aattcaccca ctggaatacc
     1501 tgtccgttgt tcaacgattt tttccatatc tttttcggta atcacaggtg tttcctcttc
     1561 agtgagctga cgttctttca ttttttgaag tttattgatc tgatcgcgat aataagctgc
     1621 tttttcgaaa tcttcttctc gagaagctaa aactttttgt tcttctgctt cttgcagttt
     1681 tttctcgatt gttttaggat caacgatttg gatcgtcaag tttttcttag aacctgtttc
     1741 atctagcaaa tcgatggctt tatccggcaa gaagcgatct tggacgtaac gattggataa
     1801 agttgcggct gcttcgatgg cctcatcagt atattttaca tgatgatagt cttcataacg
     1861 tttttgcaaa cctttcaaaa tactgatcgt ttcttcgaca gttggttcat ctacccgtac
     1921 aggttgcata cgacgttcta aagcagcgtc tttttcgatg atgcggtact catttaaggt
     1981 agtagcacca accatttgca attctcctcg agctaaagct ggttttaaaa tattaccagc
     2041 atccatattt ccatcaccag cagaaccagc accgactatt tcgtgaactt catctatgaa
     2101 taggatcaca ttttctgctt gacggatctc ttcaatcaat ttttgcatac gttcttcaaa
     2161 ttgtcctcgt ataccagtac cttggactaa agatacgaca tctaaacgaa tgacttcttt
     2221 gtccattaat ttttgcggta catcaccatc tacgattttt tgtgctaaac cttcaacaac
     2281 tgctgtttta ccaacacctg gttctccaat caagacagga ttgttcttgg ttcggcgatt
     2341 taaaatttca atgactcgtt tgatttcgtc atcgcgtccg atcacaggat cgatatcacc
     2401 ttgtcgtgct tgttctgtga tattgattcc gtattctccc aataaacctt ctgtattgcc
     2461 ttgaggtggc tgaccaccat tgaagttgtt gtttccgcca aattgtgttg gtggtgtttg
     2521 ctcataattt ccttgctgtt gcatttggcg agacatggaa cggaaaagat cgtccaaact
     2581 gccaaatcca aatggatcat tttgggccat attagtcaga cctccatttt gttgattttt
     2641 gattttttga taacagctct gacagtaatc gagttgtgtc cgctgaccat tgacagtggc
     2701 atacaagtga atcgttgcct cattcttgcc gcagttttga caaagcatga atcattcacg
     2761 tcctttcaca aaaatgggta catccatatt ttacatctcg tttttcatga ggtaaaagaa
     2821 taacccctag ttttttaaga gatttatgag gtagaaaagg tgaaatctct attgaccatt
     2881 cctgaccttt gactcttatt atactaaaaa taaaaaggga agcaagtaat tcgtttcata
     2941 tgaaaacaaa gagggtcaaa ggaaagaatg ataaacgtca ggagaagaga aagcgacaag
     3001 aaaaaggaaa taaacacgtt tgttataaaa tgtgaagaaa aggatgaaaa agaatccgga
     3061 ataatttgaa tacttttatc tgaaattgaa tgaaaactgt tgtatctaac gaaaaaaaat
     3121 gttaatcttt acagatgaaa gggtggcatt tgttaggatt ctctgaatta ctaaaaaaga
     3181 aaaccctcac ataaaaacaa cactcactta aaggagaccg attaatatgg aaaagaaaga
     3241 atttcacgta gtagcagaaa ctgggatcca cgctcgtcca gctacactat tagtacaaac
     3301 agcaagcaaa tttaactctg atgtaaactt agagtataaa ggtaaatcag taaacttgaa
     3361 atcaatcatg ggcgttatgt ctttaggtgt aggtcaaggt tctgatgtta ctattactgc
     3421 agaaggtgct gacgaagctg atgcaatggc agcaatcgtt gaaacaatga agaaagaagg
     3481 cttatctgaa taatggttga aatgctaaaa gggattgccg ctagtgacgg tgtagccgtt
     3541 gcaaaagctt acctgctagt tcaaccggat ttaacattca gtaaggcaac agtagaagat
     3601 actgcggctg aagaagctcg tttggatgct gcattagcta aatctactga agaattgcag
     3661 caaattcgtg aaaaagcagc gcaaagctta ggtgaagcag aagcgcaagt atttgatgcc
     3721 catttgatgg ttctttcaga tccagaaatg gtaggtcaaa tcaagcaaaa tattaaagac
     3781 aacagcgtaa atgctgaatc agcgctaaaa gaagtaacgg atatgtatat cggtatgttc
     3841 gaagcaatgg aagataatgc ttatatgcaa gagcgtgctg cagatattcg tgacgttgct
     3901 aaacgtattt tagcgcactt attaggcgtt actttaccaa atccatcaat gatcaacgaa
     3961 gaagtggtcg ttgttgcaca tgacttgact ccaagtgata cagcacaatt agatcgcaat
     4021 tttgtaaaag cgtttgtaac agacattggt ggacgtactt cacattctgc tatcatggca
     4081 cgttctttag aaattccagc aatcgttggg acaaaagaaa ttactgctaa agtcaaagaa
     4141 ggcgatattt tggctgtcaa cgggatcgaa ggcgatgtaa tcatcgatcc tacagatgaa
     4201 caaaaagctg aatttgaaaa agcaggtgca gattacgcag cacaaaaagc agaatgggaa
     4261 aaattaaaaa atgcagaaac tgtcacagct gacggcaagc attttgaatt agctgcaaac
     4321 atcggaacac caaaagatct agttggtgtc cataataatg gtggagaagc agttggtctg
     4381 taccgtactg aattcctgta tatggattct ccagacttcc caacagaaga tgaccaatat
     4441 gaagcttaca aagctgtatt agaaggtatg gaaggaaaac ctgtagttgt tcgtacaatg
     4501 gacatcggtg gagataaaga actaccatat ctgcaattac ctcatgaaat gaatccattc
     4561 ttaggatatc gtgcattgcg tatcagttta tctgaacaag gcgacgaaat gttccgtaca
     4621 caaatgcgtg cattgcttcg tgcatctgtt catggtaatt tacgtatcat gttccctatg
     4681 gtagctacac tcaaagaatt ccgcgcagca aaagcgatct ttgaagaaga aaaacaaaaa
     4741 ttgataagtg aaggcaagga agtatctgat acgatccaag tcggcatcat gatcgagatt
     4801 ccagcagcag cagtcttagc agacaagttt gcaaaagaag tagatttctt ctctgtaggt
     4861 acgaacgatt tgattcaata cacaatggca gcagaccgta tgaacgaacg cgtttcttac
     4921 ttgtaccaac catataaccc atcaatttta cgtttgatca aaaatgtaat tgatgcagca
     4981 cacgctgaag gaaaatgggc aggtatgtgt ggagaaatgg ctggagatca aacagctgtt
     5041 ccattgctag taggtatggg tctagatgag ttctcaatga gtgcgacatc tattcttaaa
     5101 acgcgtagtt taatgaaacg cttggacaca gcgaaaatgg ctgaacttgc tgatcgtgca
     5161 ttgaaagaat gcgatacgat ggaagaagtc gttgaacttg ttcatgaata tgtaaaataa
     5221 a
//
LOCUS       DAZKLA010000153         5203 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000153 DAZKLA010000000
VERSION     DAZKLA010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.156"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..1133
                     /locus_tag="ACG85N_002594"
     CDS             15..1133
                     /locus_tag="ACG85N_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0225305.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1261..2637
                     /locus_tag="ACG85N_002595"
     CDS             1261..2637
                     /locus_tag="ACG85N_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0225306.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3038..4618
                     /locus_tag="ACG85N_002596"
     CDS             3038..4618
                     /locus_tag="ACG85N_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFQ0225307.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            4816..5040
                     /locus_tag="ACG85N_002597"
     CDS             4816..5040
                     /locus_tag="ACG85N_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFQ0225308.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT         1748 a          845 c         1083 g         1527 t
ORIGIN      
        1 aaggaggaaa tctattgttt gaaaaactta gacagtctaa actaatgttt tggtcgcttg
       61 aattattgat catagctgca ttgatcatga tttcatcaga aataaacttt attttcgaac
      121 cgatcgggac atttttttcc acgttatttg cacctgtact gattgcaggc tttctttact
      181 atttattgaa tcctattgtc aagctgttga tgaaagcaaa aatcaaaaga atatgggctg
      241 tcgcaattgt gctgctcctt ttagttgcag caattatttg gattttactt agtgtcatcc
      301 caagtctagt acagcaaatt tcctcattgg catctaatat gcctgatttc atcaaacagg
      361 tagaaagctg gttaaaagag gtagccgaat tgcctttgtt caaggaagta gatatcaata
      421 aatattttga gcaattagac atttcttacg ggacaatcat tcagcaattc cttagtggag
      481 tatccaatag tttaggttcg atcgttggaa cgatcgcttc ggcaaccgta gtaatcatta
      541 cagcaccatt catcttgttc tatatgttga aagacggaga aaaactagtg ccaaacatcc
      601 aacagttttt cccagaaaaa agaagaaacc aaattgtgga gttattgggg caactgaata
      661 aaacattggc taattacatt agcggacaag caatcgaatg tttattcgtt ggtaccttca
      721 catttttagg atatatggta attggtgtgg attatgcttt cttatttggg gtcattgcgg
      781 gattgacaaa cctaattcca tatttaggac cttatctagg actagcacct gcatttctag
      841 taacagtctt caatgatccg gtaaaagcat tactttgttg tgtggttgtg ctaatcgtcc
      901 aacagttgga tgggaatatc atttatccta atgtaattgg gaaatcactg aaaatccatc
      961 cattaacgat cattatcgtt cttctagtag ctggaaatat tgccggattg cttggtatct
     1021 tcttaggtgt tcctttttac gcaatctgca agacgatcat ttcctatata atcaaaatcg
     1081 tcaaagaaga caagcaaaat gaaaacaaga agaaaattgc tacgacctcg taggtagttt
     1141 tagtacactt gtttttattg caaagaaaat tgctttgtga taccatttta acgtggctat
     1201 agtgccacgt ttttttctat cgtaaactaa aaattaattt tataaggaga gaatgcgatt
     1261 atgaatgctg accctgagag tcagtcgctg ttagcgcaga ttttattatt gatcgttttg
     1321 acattgatca atgctttttt agcagcttcc gaaattgctg ttgtttcgat caataagaat
     1381 cgaatcgagc aaaaagctga tgaaggagat gtgaaatctc aaaagctttt aaaaatattg
     1441 cagaatccaa ataatttcct atcgacaatc caagtaggaa tcactttggt caacattttg
     1501 tctggtgctt cactagctaa tactttatcg gaacggttag ctccggtatt aggtggcggc
     1561 acagcggcaa aaaatattgc aaatatcatt gtgctggcta ttttgaccta tgtatcgatt
     1621 gtttttggag aattgtaccc taaacgtatt gctttaaata aatcagaaga agtcgcaaag
     1681 ttcacttcag ggctgatacg cttgattggt gtagtagcta agcccttcgt ctggctgctt
     1741 tctgcttcca caagtttatt ggcgcgtatc acgccgatga cttttgatga tgaagattca
     1801 aaaatgacca gagatgaaat gcgttatatg cttgaaaacg aaggcgtatt gaataatgaa
     1861 gaattagaaa tgctccaagg ggttttttct cttgatacga aagttgcccg agaagtcatg
     1921 gtcccacgaa cggatgcatt tatgattgat atcaacgaca ctgtggaaga aaatgtgaat
     1981 gaagttttat ccgaaaacta ttcaagaatc cctgtttata acgaagacaa agataaagta
     2041 gtcggaatcc ttcatacaaa gaatttacta aaagctgccc ataaattcgg ctttgataat
     2101 ttagatatca agaaaatcat gcaggagcct ttattcgttc ctgaaacggt ctttattgat
     2161 gatctgttat atgaaatgaa aaagacgcag aatcagatgg ctattttatt agatgaatat
     2221 ggcggcgtcg ttgggttagc aacactagaa gaccttttag aagaaatcgt cggagaaatc
     2281 gatgatgaat cagatgaagt tgagaatctt tatgaaaaaa tcgatgaaca cgaatatatc
     2341 atccaaggac gtatgctgat tgatgagttc aatgaagctt ttgacagtga tctgcatatg
     2401 agtgatgtag atacgatggc tggttatttg attacagcat taggcatgat tccggatgaa
     2461 ggcgaaaagt tatcgtttga tgtcgataat attacgctgg tttctgaaga aatggaaggt
     2521 tctcgaatcc ttaaaatccg tgtcattttt catgatccgg aagaaacaga agcggaacct
     2581 gaagaagaac gaagatactt cagaaaagaa ttcgaagatg acgagccaag aagataattg
     2641 gaaacttggt tatgaaagac gtgttgcttg aataaacaag caacacgtct tttcttttag
     2701 tcagctgaca aaaaaatatt tgactcttat ggtttggtgg ctttttcatt cgctgaatcc
     2761 atgctatact gattcagttg tagtagttga caaaggttgt gaaaaagcta tcctgcatta
     2821 agtaataaga gcagccaagc aaaaacagct tttcgtattt ttggcttggg tgggcttatt
     2881 gctgataggc tgtctctaga acgccgttta ctcggaaaaa gagactgcta cacggctttt
     2941 gtcacagccc tatatgaaat tgtctttctt taagagatcg gacaatctgt gcctagagaa
     3001 aatataaata attgaatgga agaaatggag ttaggttatg aataatccaa aattaaagca
     3061 acaagtggac aatcgcagaa cttttgcgat catttcccat ccggatgctg ggaaaacaac
     3121 gattacagaa caattactat tgtttggtgg ggctatccgt caagccggga ccgtaaaagg
     3181 caaaaaaaca ggtaattttg ccaaatcaga ctggatggaa atcgagaaac aacgtgggat
     3241 ttctgttact agttctgtta tgcagtttga ttatcaaggg aaacgagtaa atatcttgga
     3301 tacaccagga catgaagatt tttctgaaga tacttatcgt actttgatgg ccgtggatag
     3361 tgcggtcatg gtaatcgata gtgcaaaagg gatcgaagca cagacaaaga aattattcca
     3421 agttgtaaaa aaacgtggca tcccgatatt tacttttatc aacaaactag atcgtgatgg
     3481 tcgtgaacca ttggaattgt tagaagaatt agaagagcta ttagatatcg aatcttatcc
     3541 gatgaattgg ccaatcggta tgggtaaagg gctagaaggt ctttatgata tctaccataa
     3601 tcgtgtagag ttttatcgtc cggaaaatta tcaggatgaa cgattagtag aattggatga
     3661 agatggactg cttcccgaaa atcatcctct aactgaaaat tccttatatg aacaagtctt
     3721 agaagaagta gaactaatca aagaggctgg agatgcattt aatcaagaaa aaattgctcg
     3781 tggcgatcag acaccagtat tttttggttc tgcattgact aacttcggtg tacagacttt
     3841 tttagagact ttcgttgaat tggcaccagc tccttatgga cacaaaacac aagatgatca
     3901 gatggtaagt ccttatgagg aagaattttc aggtttcgta ttcaagatcc aagcaaatat
     3961 gaatccagcc catcgtgacc gaatcgcttt tgtccgaatc tgttctggaa cattcgaacg
     4021 aggaatggat gtctggttgg aacgtacaaa taaaaaactg aaattaagca atgtcacaca
     4081 atttatggca gattcgagag aaaacgtcga aaaagcagta gcaggcgata tcatcggtgt
     4141 atatgataca ggcaattatc agattgggga tacattgttc gaaggaaaac taaaagttgc
     4201 ttatgaagaa ctcccttcat ttactccaga gctgtttatg aaagtgactg caaaaaatgt
     4261 catgaaacaa aaatccttcc acaaaggaat ctatcaatta gttcaagaag gagcgattca
     4321 gctttataaa acatatctaa cggaagaata tatcattgga gctgttggtc agctacaatt
     4381 cgaagtattc caatatcgta tgttaaatga gtataacgcc gaagtaatca tgtctccaat
     4441 gggacacaaa atcgctcgct ggatcgatcc agaagatttg gatgagaaga tgagttcaag
     4501 tcgcaatatc ttggctcgtg accgtttcga ccaaccgttg ttcttattcg agaaccaatt
     4561 tgcagaacgc tggtttgcgg ataaatatcc caatgtaaaa ttaaaaagtt tgatgtaaag
     4621 aagtcttgac acaagctgcc ctgcatcaag taataagaat agccaaacaa aaacagcctc
     4681 tcatattttg tttagagccg tgacggattt tccgtgacgg ctctttttac tatattaaaa
     4741 tcatagaaat gtctaatcaa aaaaatataa aaattatttt gcgtacatat gattaaagaa
     4801 aggagagtgt gacacatgga aacagaatgg caaagacgtg aaagaaaaaa acagaaaaaa
     4861 gaagagatgt tacgattatg tcgattatta gatgtaaaag actgggacaa aaacgagcaa
     4921 attgcacaaa aagtacgatc aatcatccat aaagaggaaa aagttaaaaa taaagacacg
     4981 cgtagaactg aaaatgaaaa gagaaaagta caaaagaaaa gagaaaaatc aaaaagctag
     5041 aaagaaaaag aggctgagac agaagcgttt aactccaaga aataagctgg aattcacaaa
     5101 aatttgaaaa gcaattttcg tgaatttcag cttatttccg aaggagttgc ttctgctttc
     5161 gccgtttata cgtgtttaga gcgtgagaca aaagtgttct tta
//
LOCUS       DAZKLA010000154         5189 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000154 DAZKLA010000000
VERSION     DAZKLA010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.157"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..748
                     /locus_tag="ACG85N_002598"
     CDS             137..748
                     /locus_tag="ACG85N_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0225309.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="ACG85N_002599"
     CDS             821..1750
                     /locus_tag="ACG85N_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFQ0225310.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1887..2645
                     /locus_tag="ACG85N_002600"
                     /pseudo
     CDS             1887..2645
                     /locus_tag="ACG85N_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
     gene            complement(2693..3895)
                     /locus_tag="ACG85N_002601"
     CDS             complement(2693..3895)
                     /locus_tag="ACG85N_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0225311.1"
                     /translation="MHRKEDFHMNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKT
                     ELTAFLDYEKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYR
                     RTNDTLEETVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERE
                     LHDRYAAIYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEI
                     LIDLQERGVKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKE
                     VCDDFKMVYQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKT
                     IYSTNLIESFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTD
                     TLASMFTE"
     gene            3975..4499
                     /locus_tag="ACG85N_002602"
                     /pseudo
     CDS             3975..4499
                     /locus_tag="ACG85N_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4706..>5189)
                     /locus_tag="ACG85N_002603"
     CDS             complement(4706..>5189)
                     /locus_tag="ACG85N_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0225312.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1639 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttt
//
LOCUS       DAZKLA010000155         5163 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000155 DAZKLA010000000
VERSION     DAZKLA010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.158"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..499)
                     /locus_tag="ACG85N_002604"
     CDS             complement(41..499)
                     /locus_tag="ACG85N_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0225313.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(512..1843)
                     /locus_tag="ACG85N_002605"
     CDS             complement(512..1843)
                     /locus_tag="ACG85N_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFQ0225314.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1854..3296)
                     /locus_tag="ACG85N_002606"
     CDS             complement(1854..3296)
                     /locus_tag="ACG85N_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0225315.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3283..4047)
                     /locus_tag="ACG85N_002607"
     CDS             complement(3283..4047)
                     /locus_tag="ACG85N_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFQ0225316.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4151..5026)
                     /locus_tag="ACG85N_002608"
     CDS             complement(4151..5026)
                     /locus_tag="ACG85N_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0225317.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         1565 a         1096 c          753 g         1749 t
ORIGIN      
        1 acccataaat atcaccctat ttaacttttt tcatcgtaca ctaaaattca aaacaactat
       61 ctttaatctt tacttcttgg ttttgtttta ataaatttcc ttcaaaaatg cctggagaaa
      121 ctacaatgat caccgtatct ttttgtgctt gctccaactt cttcaaatct ataatagcta
      181 acagtgtccc ttcttttacc tcctggttac tttcaacccg tatctgaaac ggacttcctt
      241 cgagttcaac tgtatctaaa cccatatgga tcagtatttt tctgccagat tcactttcga
      301 tcgttatggc atgtcgggta ggaaatatat tatcgattat tcctttaact ggcgcatgga
      361 tatatccatc atgattcgtt atagcgtatc caactcctat catttttgaa gaaaagacct
      421 cgtcttttac tgctgttaat ggaataattt gaccactagc tacagctttt agaatgtttc
      481 ttgttttaaa gaaatgcact ttttatcacc tctactttaa tgctggccaa tattttttgt
      541 ttactttgat catttcatct agtatttttt ttgcgactct ggcatttggt acggtttgat
      601 tcaatgtaaa tgcttgtaaa gctttttgat aagaaccttc aaagtaagca tctactagta
      661 acttctctac tgcaacttgt gcctccatca atcctttatg aaaatcactg attggttttc
      721 ttagtgtaat tggctccaca ccagttgcac caacatatgc cggtatttca acaactgcat
      781 ctgaccgtag attcggaatc gccccttgat taggaacgat tagcataaag cgacgacgat
      841 catcattcaa caaggaaata gctatatcga cgatatattg tccatgctcc ccaaaattaa
      901 agttcaatac ttcttccaca ataccgcggc gtattttatc agccatttct ttggtatttt
      961 tctctcgtcc atccatgacc atattagcac gtgtatactc tttgtcagta tgctcaacga
     1021 ccatatcagg atataagtaa tactctaagt aattattcgg gatattactt ggaaaatttt
     1081 tcgtgatgac tgacatcatt tgaaaggttt tttgccaagt tttatctcct atattaaaat
     1141 cggcgacttg catttctttt gttattagtt tttcaataat ctctggcata atttctcgtt
     1201 gcaattcttt atcataaatc gatgtatacc agccaaagtg gtttaaacca taatacgtaa
     1261 cgatccaatt cttacgatca taaccgtaat tcactgcaat cgtttcttca attgaaatag
     1321 tcatatcaca ggcattaatc atttttacat tcggatattt acgacgaaca gcttcagaaa
     1381 cgatcgactc tggatttgta taattcaaaa tccatgcttt gggggcatag tcttgtatat
     1441 gatcgacaat ttccaataaa cctttgattg agcgcatccc ataagcaaat cctccgagtc
     1501 cacacgtttc ttgtcctact aatccatgtt taagtggaat cttttcgtcc ttttctcgca
     1561 tctccaatcc tccaacacga atttgtgaga aaacaaaatc gcatcctgta aaagcaagct
     1621 ttggatcatc cgttgcacga atgcgaatat ttgaaaaacc ttttcttttc agcataaatt
     1681 taacaatcaa gtacatatcc atatttcttt cttcatcaat atcatataaa cgtatttcag
     1741 ataatggcaa ccgtttttca ttgttcaaaa tggcttgaac gattcctggt gtatacgtac
     1801 tcccgcctcc agctatagtg ataatctgtt ttttcatcat catcttttcc tttctattct
     1861 tctaataatt tattgaattc tgccataaca taagtcactt ctaagccgat gataatttga
     1921 taattttttt cagttggttt aatgatcccg cttatcccta attgcttgat tcgctgttcc
     1981 tcgaccatgg tgggatccac tacctctacc cgtaggcgtg tattacaact atagacatcc
     2041 aggatattgg cttttcctcc aaacgcttgt agcatttttg ttgccatata ttcataattt
     2101 ttacttgcta accctaactc tgtttcttct aaggttactt cattgccaaa ctctgcctta
     2161 gcttcacgtc ctaatgtctg aatgtttctc ttattgatca aataataaaa agtaacgtaa
     2221 tacaaagcaa aaaagaaaat tccgattgga agaatcaaaa tagcattggt agcgattcga
     2281 aaattgatga gataatcaac aatacatgct ccgaatgtaa aaccaattcg tatattgaat
     2341 aagtaacaga caatccctgc taaaccggca aaaagtgcat ggatcacgta caacatagga
     2401 gaaagaaaca tgaaactaaa ttcaacaggc tcagtgatat ttgcaataaa tgaagttcct
     2461 gcaccgcttc ccatgattcc tttgaccatc tctttatttt ctttataagc tgctttatag
     2521 atagctagac atatagctgg tataccaaac atcattacta caaaaaaaac tgataaaaac
     2581 agtccagcct gaggatctcc gttgataaag cgtgtcatct ctcctctgaa aacttccttg
     2641 ctaggagatg tatagcttcc taaatcgtaa taaatatacg tattcaacac atggtgcagt
     2701 ccaaagggga tcagcaaacg attcaaaaac ataaatatcc ccacacctaa tgctcccata
     2761 tcaacgagca gttttgcaaa attatcaatc ccaatttgga tgagcggcca gataaagccc
     2821 aaaaaaacag ctgcgattgt ttgtagaatc atcaccatag tcaatgggaa tttttcgccc
     2881 gcaaaataag aaaaaactac tggtaatttt tgttctttaa agcgattata cgtccatgcc
     2941 gctactaaac cggaaataat tccaccaaaa atccccatat tcacttctgg gtccataatt
     3001 tctaagcctt ttttcaaggt agcaatcgct aaaaaaccag taagtgcagg tattcctttg
     3061 tccttatttt tgacaaaacc gattgcaact cctatagcaa ataagatatc caagtttcca
     3121 aaaacggcat ttccagctgc accaattact gggatgttca gcatatcgtc agctgataaa
     3181 cgcatgatca atcccgctaa tggcatcgct gcaactggaa gtagcatcgc tcttccaagc
     3241 ttctgcacat ttgatttaac attcattttt attcctcctc tattaaatat cctcatatgt
     3301 tactaactca atcaaaggat catttttcac tttttgagca aatgctgaat gaattaaaaa
     3361 ttcgacttgg tgggtacgag gatacacaaa acttgaacca tttaacaatt cctgatccaa
     3421 atacgctgcg tgacacatga cttcgatcgt tttatcttct ctcaatatca tgagtaaact
     3481 tgccaaatat tcttccacac tcgtattgat ttgtcgtttt ttttcatcag tttctcctac
     3541 gacatcgaaa caaggctcaa aataatcgac atgacgaact tcatcttttc gttcaaagtt
     3601 gctccgaacc ggcaaatcat atttttttgc taatgtaaca actacctcct gattttcatc
     3661 caaagtatga gtatgatgat gactgtctaa atgagttggt atgatccccg catgataaat
     3721 tttttgaatc tgagcattcc attcctgata cagttgatct ttgtcaatgt taaatggatg
     3781 ctgataataa gataaggatc tgaactctcc gctttcgata agagtatcga cagttttgag
     3841 gagtggtttg ccacacgtca atgttaaatg gactcctaca cctagcctag gcatttcctt
     3901 tcttagtttc acagcatgct cgaaacccgg catatttgcc atcaatgtag ttgatgtcaa
     3961 aattcctcgc tgataagcat ccataatccc ataattcaca ccatggctat agccaaaatc
     4021 atccgaatta aaaatcactt ttcccatttc attctccttc ttttttctac taaaataggc
     4081 gcaaccaaat aatcatagtc catttactat gtgtcatttg gttgcgccta tagatagtta
     4141 cgcccttatt ttaatagatt cttgctagat gaattgccaa atagatgatt tcattttctt
     4201 gaatggcaag ttgaaattgc tgttcgatga tatctttaat ctttagtgct gtacaatacg
     4261 ctttgctatg tttaaatgtt tcagtaaaaa aaccaatatt ttctactggc gtttcttcct
     4321 gataaatcaa gcgtttaacc aggaatttga tatgaaccat taagcgatga taagtcagtg
     4381 attctttatc ataaacgttt gaataggatt tttctagatg ggacaaaatc gtggtcgtca
     4441 tttctactat ttttaagctc tttaaactat taggctctcc tagctcagca ttaatgatat
     4501 gcatagtgat gaatcctact tcatttgacc ctaactcggt ttgatatcga tgattgattc
     4561 gctcgaccac ttctactgct atatcatatt catttggata aaggaatctc atttctggca
     4621 acataattgc tgtgacatct ctatcttctc tttttcgttg aatagcaaaa taaatatgat
     4681 ctgtcaatgt aatatagata tttgaatcta atggagagga gatcctagct gttatcagtt
     4741 caatcacttc ttccgcaaaa tccataaatt cgattggaat ctgcgagact atctcactga
     4801 atcgtttgat cttttcttta gaatctaata caaataattt gtcagcatcc tcaacatcaa
     4861 atggcttact cactttagca gaaaagccaa tccctttccc catcgcaatt ttttctattc
     4921 catcgtcatc gatcaataca gcattatgat tcagcttttt catcacatac accgttaatt
     4981 cacctcctct atatgataaa acaaaacaac ttgcagactc cttcatacat atttttgtct
     5041 ccaaacaatt tttaaagaca aaaaacgacc taagaaagaa ccctttctta ggtcgtgcct
     5101 aattgaatag tagcacccta cataatttac aaaaatagtt taacacataa taaaagcgtt
     5161 atc
//
LOCUS       DAZKLA010000156         5106 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000156 DAZKLA010000000
VERSION     DAZKLA010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.159"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..967)
                     /locus_tag="ACG85N_002609"
     CDS             complement(<1..967)
                     /locus_tag="ACG85N_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HFQ0225318.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLE"
     gene            complement(971..1381)
                     /locus_tag="ACG85N_002610"
     CDS             complement(971..1381)
                     /locus_tag="ACG85N_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0225319.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(1399..1890)
                     /locus_tag="ACG85N_002611"
     CDS             complement(1399..1890)
                     /locus_tag="ACG85N_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0225320.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(1900..2691)
                     /locus_tag="ACG85N_002612"
     CDS             complement(1900..2691)
                     /locus_tag="ACG85N_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0225321.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(2684..3457)
                     /locus_tag="ACG85N_002613"
     CDS             complement(2684..3457)
                     /locus_tag="ACG85N_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0225322.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(3613..>5106)
                     /locus_tag="ACG85N_002614"
     CDS             complement(3613..>5106)
                     /locus_tag="ACG85N_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0225323.1"
                     /translation="KSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLES
                     DEFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKF
                     RMKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITA
                     SAIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQ
                     MYEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGL
                     SQKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTI
                     CVLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGAST
                     KLRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEH
                     LKEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
BASE COUNT         1626 a         1027 c          665 g         1788 t
ORIGIN      
        1 tctctaactt aaaacttgaa aatggagtga tttctttaaa attctcaatt attttttcag
       61 gcagtttaac accaagagtt ttgtctgtct tttgaataat ttgatgtgga acaagtgcac
      121 cgccccaatc ccagttattt tgatcgttct ccccttcttt tgttgccacc cagccagtta
      181 gaaaacggcc agtttcatta gttccgactg ttcttgctgc ataataggct tttccgtcga
      241 aagcttcgtc tactggtgat ttccaatctg aaaataatcc tgctccgact cgatatttag
      301 tctttttatc ttcacaatac tctgaaaaaa gtaaatacca cgttccatca atttcaaata
      361 tatcaggcat ttcaatcatg ttaaattcat tattagccca gtagtctcct ttgaaattcc
      421 aatcttttaa atcttcggat tcaaaataga ccaatctccc cgtcggcttt ttcttacttg
      481 ctggttttct agctcctagt atcaaaacgt attttccatg atcttgatca aaaattgcaa
      541 aaggatcgcg ccaatcattc ttatcatatc cttcttgagg aacaagtttt gctgcttcgc
      601 catccttttt ccaatgaatt aaatctttag aacttgcaat cattaacact tctgaagttt
      661 ttccatccaa cttagctttg cgattatgtc cagtatacaa agctaaatac tcattgattc
      721 cttggacaat acttccagcg taaatgtatt gatcaatgtc atcgttgcca ccacgatgaa
      781 ttacttcccc gtattcttca aatttcacaa aatcagtggt tgtaactagt gaccacccaa
      841 aaggatcagt taaaggttta ggatttctcg tatctcgttg atgaaataga taaaaagttc
      901 catctttata aaaaggcatg caatccccaa accaaaaatc tttaggcttg taaaaaattt
      961 tttgcattta tcactcatcc tcttcgttag tatgtctttg catatctgca agacacgcaa
     1021 ttgtattcat atttcttaga ttttcgatga tatcatcgct tttttttcct tgtaatctca
     1081 aattgacagc ctcaatcaaa attcctaaat tcatgccacc aagaagttga aacccatttt
     1141 gaaatacttc acttctttta gcaaattcgt tgaaaggtgt tccaccaatc aagtcagcaa
     1201 ataccataac ttcttgattt tttacttgag aaactatttt ttcaatgttt tcacggaatt
     1261 cttcaatgcc tgcatctcca agtgttacca catgacaatt taaattatcg ccaaaaaaca
     1321 tttttaatgt atctacaaat ccaatggcaa tatttccatg agatgtaatt ataaactcca
     1381 tccactcatc ctctgttctt aataaataaa atcctttaaa aattcttttg aatctctagg
     1441 agtttgtatc actgtcactt caactccatc actaattagt tctttaaaag cctctctctc
     1501 ttcttcagta atatttatcg tcggacggat gcgttctgtt ccattccttc ttgataaatt
     1561 tcctacgttt acacttgtta ttggtaaacc gttatcaatc aaaaatttaa catcgattgg
     1621 agaaactgca acaataagaa ctttttgtcc ttcatattta ccagaaagaa tatttttaac
     1681 tgctacttcc cttgtaatta ttgaagttct aacattaggt ggtgcagcga ggcgaactac
     1741 agcttttttt acgtcatcat tggctacttc attattaata accataattc tgttagcact
     1801 caattgattt gtccattgtg ttgctacttg tccatgaatc attcgatcat caatacgaac
     1861 atgtataata cctttcattt tttcatccat attcaacacc tacttcaaaa tatttaacgc
     1921 tcttaacgca atagaaacta ctaagattaa tagaattaca taaactggtt taacctttcg
     1981 tcctaacaaa taatacacaa ttgctactac acatgcaggt aataaaccag gcataacttg
     2041 atcaaagatt tcatttccgg tcattgttaa gtcgccttgt ttaaattcaa agccaacatt
     2101 cacttttaca actgtggcaa tgagtgcacc gaccaccatt aaccctaaaa ttgatactgc
     2161 atcagtaata gactttaatt ttgtttcaat ttgagttagt aggctcgcac ccgattcttt
     2221 cccagcaagg tataaaggat aagcagtaat ccttaataaa attgatgcca gtatccacaa
     2281 aataattcca acaaaactac cttccaatgc catattagct gcaattgctc caaaaatagt
     2341 ccatggaata actacaaata cactatctcc aattcccgct aaaggaccca ttagacctgt
     2401 tttcagagca cttactgctt ccaaagactt atttttttct tctttttcaa ttccaacatt
     2461 cataccaata atgtaaggcg ctgtatacgg cgtagtgtta aaaaattgta ggtgagctaa
     2521 aacggattgt ttcattcctt caggatcatc tttatatgtg tccttgataa aaggaagcat
     2581 agaatacgca taacccaacc cttccatttt ttcataattt aatgaagttg caccattgag
     2641 aatccaccgg aaaattactt gtttgtcgat ttttgtcagt ttcttattca tcttcgtcaa
     2701 aacctcctgt tgcagctggt gctacttgag tattatttga tctattttta tatacgatta
     2761 gtgcgattgc agcgccaagt aacgaaatcc ctaaaattgg tatttggaaa taaactgcca
     2821 aaacgaaacc aactaacaaa taggcaaagt aatcttttgt aggtaaatat ttaagtagca
     2881 ttcctatccc aacagcaggt aatagtcccc ctgctacctt taaaccaccg gtaacccatt
     2941 caggaatata attgattgca cttcccacta acgttgggcc taatatcaca gttaacaaaa
     3001 caggaattcc agatacaaag cttgtattaa ttgatgccaa atattgcatc cactctaatt
     3061 tttgataatc ttctttttgt gcgaattttt ctgccttttg ttgaaaaaaa atgtttgttg
     3121 accactttag aacatctagt tgtacaatta acattgctac cggtattgct aaagttattc
     3181 ctatagaagc atcctggtta gttgagattg ctaaataagt accgatcaat gctgctgttt
     3241 gataatccgg tactgatgca ccaccaaaac tagtaatacc tagtgtcatt aattgcaaag
     3301 tcccaccgat ataaagtcca gtttgaatat ctcccataat aagtccggtc aagaaacctg
     3361 caacgacagg ttgggaggtt ccgattttgg gcccttcctt atcccagtta atgaaaaatc
     3421 catacaacac aactaaaaat gtttgccaaa acgccatttt gttctctcct ttcttcttat
     3481 acttttatat aagcaattac tgtgccaact aacattatcc aataaagcgg gaaaatacag
     3541 taaatattaa ttaaatgagc gtctaaatat actttggaca ccctttcatt tttttagacg
     3601 ctagtatgct atttagaaaa tagtctgctc aaaaattgta tagattaact catactctcc
     3661 tttgggtatt tcaatacagt aaatatcttt gatttctgaa aaaaaatctt ttgatacatt
     3721 taaaaaagta tcaaatagtt gcgctccctc tttcaaatgt tcatttgtta ttccatcacc
     3781 aatcataatt cgttcaatca ttccagaaag atgtagaaat agattaattc tcagaaaact
     3841 tgggattttg acatcgtaat atttttcata caaatatagt actttttcta tctcattgaa
     3901 tacttttttg gaatctaaaa agcgaagctt agtactagcg ccttcaatag taaagagttt
     3961 aactaattca tttaagcaga actcacagct atccgagtcc atatacttat ttaatttttc
     4021 taaggtttga ttcccattaa ttagattttc taaatttaaa caatcgattc cttgaatgta
     4081 aaattcagtg gttgaaagaa cacaaattgt attcttaaaa atcatagtat ctttttcctt
     4141 tgctattttc tttaatgcgt taaaatcaaa aatcaaaatc tccacttgat tatcgagata
     4201 ccgttttaaa atatccttaa ctttttcaga aatcccctca ccagagagac aagatactat
     4261 tacatttttc ttttgtgata atcctttata atactgaact ttaacttgaa acgaatcaaa
     4321 atccatctgg taaaaatgac taattggctt tttttggcaa acttgaatcc cacattcaat
     4381 cgctaaagct gtagaaacat tgtttaaaat taccaaatca cctattacat tggattcaat
     4441 tttttcatac atttgttcca atgatcccat atcaactagc agaatgagtc cattcgtcgt
     4501 atctatactt ttacaaaaat tgttgattag gttaattaag tcactagtct caccttcaat
     4561 tggcatatcg aaagcctcat aaacatatgt attcagcaac ttatttgttt cccttgcgat
     4621 cgcagaagct gttattttac cgtgcataac tatcagacaa tgggtttctg gtattttcac
     4681 atagaacttc tttacaagaa aaagtatcca tttcaacaaa agttcttgtt tactttcttc
     4741 gacttctaaa ctactccaaa tctcatttgc caataatttt tgtttatcat ttttcatacg
     4801 aaattttctg aacttagaca gcaaaaaatt tcttgatgtt tcttcaatta catcaataaa
     4861 cattataaat agactgatat ccgccaagaa ttcatctaat aaacatagac ctgtcgaatc
     4921 aataattggt gatattttat taacaacata gtttcttaat gctataaatt cgtcactttc
     4981 taaataatta tactcagaag aacctgcaca ctctttaagt attctatctc ttaattgact
     5041 catgctttct tcaattgaaa tgtgattttg aaaaagttca atcaaatcaa tttcagaaag
     5101 tgattt
//
LOCUS       DAZKLA010000157         5006 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000157 DAZKLA010000000
VERSION     DAZKLA010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.160"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..1213
                     /locus_tag="ACG85N_002615"
     CDS             44..1213
                     /locus_tag="ACG85N_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0225324.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTP
                     EPDTDARDQGIPEKINKKTIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAET
                     LKSSKDTTFASPETKNKQLPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(1339..2418)
                     /locus_tag="ACG85N_002616"
     CDS             complement(1339..2418)
                     /locus_tag="ACG85N_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0225325.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2527..2967)
                     /locus_tag="ACG85N_002617"
     CDS             complement(2527..2967)
                     /locus_tag="ACG85N_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0225326.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3024..3434)
                     /locus_tag="ACG85N_002618"
     CDS             complement(3024..3434)
                     /locus_tag="ACG85N_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0225327.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3615..4049)
                     /locus_tag="ACG85N_002619"
     CDS             complement(3615..4049)
                     /locus_tag="ACG85N_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0225328.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(4149..4997)
                     /locus_tag="ACG85N_002620"
     CDS             complement(4149..4997)
                     /locus_tag="ACG85N_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0225329.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         1828 a          963 c          849 g         1366 t
ORIGIN      
        1 atcttatttc ttgcatacaa aaaaatcaag gagtgagaga gttatgagat attcaaccat
       61 cttaatatca acatttgcag ccttaagctt atcgcttact ataaatcaga cagtccacac
      121 agaggagata gaaacagcca aatggacaga aaccaagtgg gccaacgtca atcgaattac
      181 ctttcaggat gaattttcat ccttcaattc aggagtcgca aaattaagaa aaggacaaac
      241 agcgaccatc aaagcaaaaa ttgactattc aggagactta gcacctatac tacaatcagc
      301 tgttacattt gagaatgttg atcccgcttt gtcgattggc tatgacgaga acactttgac
      361 tatccaacac aagcaagcga tatttacctt caccgttaca ttaaatcagg acttgttaaa
      421 accagcgctt tttactgtca aggtatcaga tggactaccg aacgatcatc atcatttaac
      481 gccgtatagt cagcggcaga caattgagca agattcagct atcgactcag gaggagatct
      541 tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac cgcctacaga
      601 aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg atacagaaac
      661 tcaaccagag ccagacaatg aatcaaaacc tgaaataacc cctggagaaa aaccagatgt
      721 agacccagaa gaaaaaccgg atgtgactcc agaaccagat acggattctg gaaaccaaac
      781 agtaccggaa acaaatccag acacagataa tgaaactgaa aatccagaaa aactagaggt
      841 agatccagaa gaaaaaccag atgtgactcc agaaccagat acggacgcaa gagaccaagg
      901 aataccagaa aaaataaaca aaaaaacaat tcaagaggat ggaaaaaaag agagtaaaaa
      961 atcaaatcta gcaattctaa aaataaatga agagcagctg aataaaaaat cacgaatatt
     1021 tgatagcgcc caatcagctg aaaccttaaa aagtagcaag gatacaacct ttgctagtcc
     1081 tgaaacgaaa aataaacagc taccaaaatc cggagagtca caaaacaaag ttatactgtg
     1141 gagcggaatc attctattaa gtatagcaac aatgctttct gcaaaaagat ttaaacaaaa
     1201 cagatctctc taacatttga ataatgtctg tgaacgcatt tttcaaataa aaagaaatat
     1261 ccagaaaacg accccaaaat accagattga aatctgatat ttggggtcgt tcatacttta
     1321 tctttcttca tatttatttt aagggatatc tgtttctttt ggaagatggt tcgggatctt
     1381 tttcactttc aatacatcta aaaagaaggt aaagatcgga atgccgacaa tcaagcccca
     1441 aacgccaaag aaccgttcgc taacaagtaa gaccacaaat gtataaaaaa tcggcagttc
     1501 tgttttgctg gacatcaact tcggattcag cacataagac tcaattagat ggacaatcaa
     1561 aatcgtaatc aggatataaa caacatctcg gatgcctcct tgggaataag ctatgaaaga
     1621 tagaggaata caagaaatga taacacctgc tactgggatc agacttaaaa tgaaaatcat
     1681 gatcgctaaa ctcagcagct gatgaaagcc aaatacagcc aaggcgattg tagtgagcaa
     1741 cgtgtttaaa agagcgataa caaattgagc ttctaatacc aaaccaaatg tattgacaaa
     1801 cttatcggca aaatagtaaa tatcttgaaa gaaccaagaa aattcacttt ttaaaaacaa
     1861 tcttgagaat acaattgttt ttttcttttc aatcataaaa aagaaactta agatgaatga
     1921 catagcaaac gccactgcca tttttcctat atcctgtaaa tagccaagcg caatcgaagc
     1981 gccattttga agctgttcaa aaaaatcgtt tcgctcaata taagtatgga tatattgtaa
     2041 aacaggatct gcatcttttg gctgatgctc gtaaaaatca ataacggact ggatcatatc
     2101 aaatgtttga tgaataagca tcgggatata ctttgtgaat gccagataga taaggtaaac
     2161 gatcagtgta taaatcatga tagctaaaac acctgtaggg attcggacaa atcgctgaat
     2221 gatttgaaca agatgaatca tcaaataagt aaagataaat gtgagcaaaa tggttgtaat
     2281 catacttcta actaagaaaa gaacaaaaac gacaaataat aaaacagtga gacggcgtat
     2341 tttttcattc gccaaaaaac gctgataaac ggtcaaatcg acaacctcca aataacaaaa
     2401 aataaacacg ttattcatta tatgatggaa tatgaaaata agaaagagaa agagcaaaaa
     2461 gaaaaaccgc taaatgacgt tatcttatga gttggttttc ctcataaaat cgttcatcta
     2521 gcagtttcag ccctttttct ggtcttgatg aaaattatct agcatttttc gggtcagatg
     2581 ttgcagtgta tccaattctt cctgttcaag tggaagataa gacttgatct tttgtggaat
     2641 accttctgct tgtttttgca acgcagcacc ttgaggagtc agcgtaacat ttacgattct
     2701 ttcatcaccc gtttcacgct gacgattaat ataatttttt tctgctaact tttttagtaa
     2761 gggagtcagc gtaccagaat ccagacacaa atcatttcct agctctttca ctgaagtctt
     2821 tttcttttcc cataaaacta acatcacaag atactgggta taggtaagat tcagcggctt
     2881 gaggagggaa gtgtagtgtc taatgatttc cttagaggcg gcatataaag gaaaacatac
     2941 ttgattaact aaatatagat cttccatttt tccacctccg ctgttttact atagtgtatc
     3001 atagcggatt tacacaaaaa agtctattca ctaactgctt tcagagtaac ttctatattt
     3061 ccagctgtag cttttgaata cggacacact ttatgcgctg cctctaataa ttcttgcgtt
     3121 tcttccagag aaagaccttc gatagatcct tcaatctcta cacctaagac aaagctcgtt
     3181 tcactttctg aataaaggga aacttgtgca ctgacagtag aagccccaga gatatttttt
     3241 tgctgtttga tcaattctaa tgcactgtta aaacatgcac tgaaaccagc tgcaaacaat
     3301 tgttcagggt tggttgcatt ttcgacgcgt ttaccaggag ctgcaatatt caattcgaat
     3361 gtacgatcgg gattatgaac ttctccagag cgtccgcctg tgttgatcat tttcgtagaa
     3421 tacatttttt tcataaaatt accaccttta attatatttt atacaattga attttacaca
     3481 atataattgt ggaaagcaaa gaaatggatt ggcataagat aaataagaga aagcacatga
     3541 aacagacgcg ctttctcgaa tagcaaacat atacaaaaaa atatcagaga caaaaactaa
     3601 tctcatcata aacattacat atatttctca aaaagttgtt ttctttcttc aaaacttact
     3661 tcacgatgga cctgatgaag catccataaa taaccaaaag gatctctgaa cattgagtta
     3721 gtcacaccaa agtccatcat ttctgtcatt tcttgaatct ctacacagcc atttcgtaat
     3781 gctttattat gtgtgtcctc gatatcagag acgatgatat tgaaccagat cgtatttgga
     3841 tgttcagcag taggagcagt caaaccaaga gacgtgtttt catccagcat atggaatctc
     3901 gtgccataca aggtaaaaat gacttcatta ttgcctctag ggtaatctgt tgcctcaatc
     3961 agttgtattt catcaaagat tgtttgatac agttctagtg ctttcaaact atcatttaat
     4021 acaaaatcta tttccgttcc tttatgcata aagatctcct ctccttttca gtcgaacagt
     4081 tactagattc ttttattata ccagttaaag aaaacgcatt caaaaaaata gcggatttac
     4141 ttaccaactc attcgattgg atgataagta aatccgcgtc ccattacttc tgctacgctg
     4201 ctgacaatga caaaggcact tggatcgatt tcttctatca atggtttcat tcgggaaaat
     4261 tcatgatcag tcacaacgat caataaaacc tcttttgact ttcgattata cccgcctttt
     4321 gcctctagca gggtaaggcc acgtccaatc tcagcactta agcgaaggcg gatgtcttcc
     4381 aaataatgtt cactcatgac cataacagat ttctttcgat tgatacctgt ttcgatataa
     4441 gtcatgacaa ttgaagtgat tgcaatagat aaaatagcat aaagaaactc ttctattcca
     4501 aaaacaaaca gattaagaga aacaacgaat gcgtctgtaa aaaataagcc aatcgaagta
     4561 ttcaagtgga aatatttttt aaaaatgagg ggagggatcg tcgtaccgcc gctagaagag
     4621 ttgtttttat ataaaatcgc aactcctaaa gcaaaaatcg cacttccaaa aatgacagac
     4681 aacaaccgat ctgaagtaat cattgtttct ggaactattg caatgattaa aggaaaaaac
     4741 aagcttccga aaagtacttt aaaaaagggt tttttaccta aaaatagaag agctagtacc
     4801 aacataacga tattcaacac taaaataact atagctcggt caaatcctaa aacagcttcc
     4861 agtaatattc ctagaccgct gacacctcca gcagcaatat gatgaggccc taaaaacata
     4921 ttgatagcga ctcctaatat gaagagggca aaaataatct taatgtatgt ccatataaat
     4981 tgtctcgttt ctttcattta tccact
//
LOCUS       DAZKLA010000158         4979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000158 DAZKLA010000000
VERSION     DAZKLA010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.161"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..579
                     /locus_tag="ACG85N_002621"
     CDS             94..579
                     /locus_tag="ACG85N_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFQ0225330.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(683..1276)
                     /gene="clpP"
                     /locus_tag="ACG85N_002622"
     CDS             complement(683..1276)
                     /gene="clpP"
                     /locus_tag="ACG85N_002622"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFQ0225331.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            1456..1983
                     /locus_tag="ACG85N_002623"
     CDS             1456..1983
                     /locus_tag="ACG85N_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225332.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            2074..2811
                     /locus_tag="ACG85N_002624"
     CDS             2074..2811
                     /locus_tag="ACG85N_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFQ0225333.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            2815..3732
                     /gene="miaA"
                     /locus_tag="ACG85N_002625"
     CDS             2815..3732
                     /gene="miaA"
                     /locus_tag="ACG85N_002625"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFQ0225334.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            3734..4963
                     /gene="hflX"
                     /locus_tag="ACG85N_002626"
     CDS             3734..4963
                     /gene="hflX"
                     /locus_tag="ACG85N_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFQ0225335.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         1692 a          851 c         1112 g         1324 t
ORIGIN      
        1 tacctgtttt ttttcttatg aaataaatat atgctatgat acctttattc aataggatta
       61 gtttctacaa aaaatgaaag gagaaaaata aatatggaac ggtttatagc atcaaatgcc
      121 acagtgattg gtgatgtgac attgtcagaa gatgtgacga tctggtatca agctgtttta
      181 cgcggggaca gtaattggat aaaaatagga caaagaacga atatccaaga tggtacaatc
      241 atccatgtcg atcatgatgc acctgtagat attgcggaga atgtgacggt aggacatcag
      301 tgcatgctac atggctgtac aatcgaaaaa ggggcgctga tcgggatggg gaccacgata
      361 ttgaatcatg cggttattgg tgagaatagt ttgattggtg ctggatcatt agtaacagaa
      421 ggaaaagtta ttcctcctaa tgtcttggct tttggtcgcc cagctaaagt gatccgtcct
      481 ttgacaaaag aagaaataca aaaaaacaaa gaaaacatcc agcattatgt cgagataggt
      541 cggatgcatg cgcaaggaaa attcaaggag caaagatagg cagtagcatc cataaaataa
      601 aaaaggatgg tcagtttgag ttgaccatcc ttttttattt ggtttagata agagagctta
      661 aaaaagcttc ctttgaaaca gattaattta atgaagaact gttttccatg acttcatcga
      721 tcaatccata tgcttttgct tgttcagctg tcatatagtt gtcacgatct gtatcttttt
      781 caatcacttc aagcggttgt ccagtgcgtt cagctaaaat tttgtttaag cgttcgcgtg
      841 tttgcaaaat atggcgtgcc gcaatttcga tttcagttgc ttgcccttga gcgccgccaa
      901 gaggttgatg gatcatgatc tcagcatttg gtaaagcaaa tcgtttacct tttgtaccag
      961 ctgtcagcaa gaagcttccc attgaagcag ccatacccat aacaattgtt tggacatctg
     1021 ctttgacaaa gttcattgtg tcgtaaatag ccatacctgc tgtaactgaa ccaccaggag
     1081 aattgatata aagatagata tctttttcag aatcttgagc gtctaagaaa agcaattggg
     1141 cgatgatcga attagcaaga tcatctgtta cttggccgct taacatgatg atacggtctt
     1201 tcaataaacg tgagtagata tcgtaagctc tttcacctcg tgaagattgt tcaatgactg
     1261 taggaattaa attcataata gagttcctcc ttgatttcgt ttagaaatag tgtaccataa
     1321 tttagtttct aatacctaaa atgtagtata catttttggt caaaaaaggt caaataaaaa
     1381 acttaggcaa attagtcgca taaacccgct ttatggtatg ataagcagag aaacaaaaca
     1441 aggaggaaca acagattgat agttggtatt actggcctta tcggagtgct gacaggtgct
     1501 gtagttgtct atatcggcat gatactgcaa atgcgtcact tggaaaataa agaacagcgt
     1561 agacgtgaat tggaattgaa agtcaaagaa atcgagacgc tgaatcaatt aaataaaaag
     1621 atcaacgaga tcttgcaaaa acggacaacg ctgatggaag aatatgtcag ctttgatgca
     1681 tttgatgatt gttatatcac gattgacgat tttgcgtatc tccaatcatt ttcagcgcaa
     1741 aatagttttt atctgccaaa ttatatttta gacgagctat ttaagaatat ctcccatcga
     1801 aaagtgattt tatctccaga tgaaacaatg aagattggtg gatatgctta taaaggcgga
     1861 cgcgtgattt tagagaattt tacggatagt ctgactgaaa tgatcaatga aaaaaaagct
     1921 cagttgaaga ctatgacgcg ccagccgatt ggtttcttct cgaaagatga tgtttggagc
     1981 tgatattttg aagcattgcc gttgcacatg agagcgtggt aatgctttct ttttttctta
     2041 atgatacaat agaactgaac ggagtgaaga aagatgttac aaatctatgc ccatcgcgga
     2101 agcagcggca cccacccaga aaatacgctt cctgcatttg cagaagctgt acgagtaggt
     2161 gcagatggta tcgaactaga tgtccatcta agcaaagatg gttatttgat cgttatgcac
     2221 gacgaagaag tggaccggac gactaacggt aaagggctga ttcgagaaaa aacattggaa
     2281 gaactcaaaa aattgaatgc agggggcaaa ttcagcaaag aatttccagc agctaaagtt
     2341 cctgaattga gagaagtgtt aggactgctc ctccaaaaaa attaccgagg gatgctaatg
     2401 attgaaatta aaactgatca gtatgaatat cccggtatcg aagaaaaatt agccgaaaca
     2461 ttaaaaagta gagattggcc ttttcaacat gcatattgca gctttcattt ggattctttg
     2521 gaaaaaatcg cattactgga accggagata cagctggact tattgatgag tacttctgat
     2581 aaaaagatcc aactagcagc agaaacccct tatatagaag gagtacatcc aaaaaatact
     2641 tggttgttcg aacatccagt ccaggcagct gattttccaa aagcaattcg tgcatggacg
     2701 attaacgatg aagaagaaat ggccaaatgt caagaactca aaatagatgg aataataact
     2761 gattttccgg agaaagctct tcggtggaaa caatcaacga aaagtaggta aaatatggaa
     2821 aaagtattag tgatcgtcgg tccgacagca gtcggtaaaa cagcgctaag tgtggaactc
     2881 gcacaactat ttggaggaga gatcatcagt ggtgattcat tgcaaatcta taaaaatctg
     2941 gatatcggta cagcaaaaat caaagaagaa gagatgaata atgttccgca tcatttgatt
     3001 gatgtgatcg aacctactca acgttactca gccgttgatt ttcagcattc aggtcgacaa
     3061 ttgattaccg agattgcgaa ccgagggcac ctacctatca tagctggtgg tacgggtctc
     3121 tatatccaat cccttctata tgattatcag ttaggagcga cagaagaagc gaatcctgag
     3181 attagaaaaa agtatgaaac gttagctgaa acaattggtt cccaagcatt atggaatcag
     3241 ttgaaagaaa aagacccggc agctgctgag aaaatccact ggaacaatca acgcaaagtg
     3301 ataagagctc tggaagtttt agagacgaca ggacatagta tcacggcacc taaagaagag
     3361 ccaaagcaat tgtatgatta cctcatgatt ggattagata cgaagcgtag tattctttat
     3421 gaacgaatca atcaaagagt cgatttgatg ctagcagaag gtttagtagc agaagcgcgt
     3481 aaggtgtatg agcttggaga agtccaggcc agtcaaggta ttggatataa agaatttttc
     3541 ccttattttg aagggaaaga aacattggaa gaagcaacag aacaagtcaa actacattcg
     3601 agaagatacg cgaaacgtca gttgacgtgg tttcgtaacc gtttggatgc acagtggttt
     3661 gatcttataa gtgagccaaa tcaaaaaagt cagattgagc aaacaatcaa aaaatggctg
     3721 gaggcggaat aaaatggaag aaaaagtgat catcgtaggg gtagaaaccg aagcaaacca
     3781 gcgttacttt tcagagtcaa tggaagagct agttcagctg acgaataccg catcaggaga
     3841 agtcgtattc acaattacac aaaagagacc acaagtggat cggcaaacga ttatcggtaa
     3901 agggaaatta caagaattag ttcaacaagc ggatgcgcat gaagcggatt tgatcatctt
     3961 taatcatgag ctaactccga gacagagcca actgattacc gatgctgttg gtcttccggt
     4021 aattgatcgc gtgcagctta tcttagatat ttttgcgatg cgtgctcgtt cgaaagaagg
     4081 gaagctgcag gttgaacttg ctcagttaga atacctgctg cctcgtttag tagggcaagg
     4141 gaaaacatta tctcgtcttg gcggtgggat cggaacaaga gggccaggtg aaacaaagtt
     4201 agaaacagac cgccgacata tacggaataa aatttttgct gttaagaaag aattgaaaga
     4261 agtagaagct catcgagagc gcaatcgcca aaaaagaaaa aatagcgaga tttttcagat
     4321 tggacttatt ggctacacga atgccgggaa gtcaactatc ttgaatctat tgacacaagc
     4381 ggatacttat tcgaaagatc aattgtttgc aacgttagat ccattgacta agagatggcg
     4441 ttttgctgaa ggatttgaga ttactgtaac ggataccgtc gggttcatcc aagacctgcc
     4501 tacgcaactg atcgatgctt tccattctac attagaagaa agtcaaaata tggatctttt
     4561 gctacatgta gtcgatgcta gttctccaga tcgtatttta caggagcaga ctgtcttaaa
     4621 attgatggat gaactgaata tgaaagaaat gccgatactg actgtttaca ataaggcaga
     4681 ccagatcgac ccagcgatgt tcacgcctac gctattccca aatgtattga tttctactca
     4741 gacgaaagaa ggtaagatgg cgttgatcga agcaatcaaa cgtcagctga tggaactaat
     4801 gaccccttat acactttctg tgccaagcaa cgaaggacag aaattaagcg agttgagaag
     4861 acaaacaatg gttttggacg aagcgtacat ggaagaaaca aacgaatatg aagttaaagg
     4921 gtttgcaaaa accaattcaa aatggttaag aaaaaaccaa taaaatttta tttttttaa
//
LOCUS       DAZKLA010000159         4933 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000159 DAZKLA010000000
VERSION     DAZKLA010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.162"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACG85N_002627"
     CDS             85..972
                     /locus_tag="ACG85N_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0225336.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACG85N_002628"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACG85N_002628"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFQ0225337.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACG85N_002629"
     CDS             2274..2720
                     /locus_tag="ACG85N_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0225338.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACG85N_002630"
     CDS             2993..4345
                     /locus_tag="ACG85N_002630"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFQ0225339.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACG85N_002631"
     CDS             4516..4803
                     /locus_tag="ACG85N_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0225340.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DAZKLA010000160         4848 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000160 DAZKLA010000000
VERSION     DAZKLA010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.164"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..260
                     /locus_tag="ACG85N_002632"
     CDS             3..260
                     /locus_tag="ACG85N_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225341.1"
                     /translation="MEIRKLANIYTLETFKKELEKRNIYLETKSDKYFSSEGEDYIYK
                     LIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            303..1793
                     /locus_tag="ACG85N_002633"
     CDS             303..1793
                     /locus_tag="ACG85N_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0225342.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            2141..2311
                     /locus_tag="ACG85N_002634"
     CDS             2141..2311
                     /locus_tag="ACG85N_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225343.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            2325..2942
                     /locus_tag="ACG85N_002635"
     CDS             2325..2942
                     /locus_tag="ACG85N_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0225344.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            2942..3880
                     /locus_tag="ACG85N_002636"
                     /pseudo
     CDS             2942..3880
                     /locus_tag="ACG85N_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(4573..>4848)
                     /locus_tag="ACG85N_002637"
     CDS             complement(4573..>4848)
                     /locus_tag="ACG85N_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatA-like O-acetyltransferase"
                     /protein_id="HFQ0225345.1"
                     /translation="KEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNSSFLLPIIT
                     IGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
BASE COUNT         1787 a          745 c          884 g         1432 t
ORIGIN      
        1 tattggagat aagaaaatta gcaaatatct atacactaga aacgtttaag aaagagttag
       61 aaaagagaaa tatctactta gaaacaaaat cagataagta tttttcttcg gagggggaag
      121 attatatata taagttaata gaaaataaca aaataattta ttcgattagt ggaaaaaaat
      181 tgacttataa aggaaaaaaa tctttttcaa aacatgcaat attgaaacag ttgaatgaaa
      241 aagcaaacca agttaattaa acaacctatt ttataggatt tataggaaag gagaacagct
      301 gaatgaatat cccttttgtt gtagaaactg tgcttcatga cggcttgtta aagtacaaat
      361 ttaaaaatag taaaattcgc tcaatcacta ccaagccagg taaaagcaaa ggggctattt
      421 ttgcgtatcg ctcaaaatca agcatgattg gcggtcgtgg tgttgttctg acttccgagg
      481 aagcgattca agaaaatcaa gatacattta cacattggac acccaacgtt tatcgttatg
      541 gaacgtatgc agacgaaaac cgttcataca cgaaaggaca ttctgaaaac aatttaagac
      601 aaatcaatac cttctttatt gattttgata ttcacacggc aaaagaaact atttcagcaa
      661 gcgatatttt aacaaccgct attgatttag gttttatgcc tactatgatt atcaaatctg
      721 ataaaggtta tcaagcatat tttgttttag aaacgccagt ctatgtgact tcaaaatcag
      781 aatttaaatc tgtcaaagca gccaaaataa tttcgcaaaa tatccgagaa tattttggaa
      841 agtctttgcc agttgatcta acgtgtaatc attttggtat tgctcgcata ccaagaacgg
      901 acaatgtaga attttttgat cctaattacc gttattcttt caaagaatgg caagattggt
      961 ctttcaaaca aacagataat aagggcttta ctcgttcaag tctaacggtt ttaagcggta
     1021 cagaaggcaa aaaacaagta gatgaaccct ggtttaatct cttattgcac gaaacgaaat
     1081 tttcaggaga aaagggttta atagggcgta ataacgtcat gtttaccctc tctttagcct
     1141 actttagttc aggctattca atcgaaacgt gcgaatataa tatgtttgag tttaataatc
     1201 gattagatca acccttagaa gaaaaagaag taatcaaaat tgttagaagt gcctattcag
     1261 aaaactatca aggggctaat agggaataca ttaccattct ttgcaaagct tgggtatcaa
     1321 gtgatttaac cagtaaagat ttatttgtcc gtcaagggtg gtttaaattc aagaaaaaaa
     1381 gaagcgaacg tcaacgtgtt catttgtcag aatggaaaga agatttaatg gcttatatta
     1441 gcgaaaaaag cgatgtatac aagccttatt tagtgacgac caaaaaagag attagagaag
     1501 tgctaggcat tcctgaacgg acattagata aattgctgaa ggtactgaag gcgaatcagg
     1561 aaattttctt taagattaaa ccaggaagaa atggtggcat tcaacttgct agtgttaaat
     1621 cattgttgct atcgatcatt aaagtaaaaa aagaagaaaa agaaagctat ataaaggcgc
     1681 tgacaaattc ttttgactta gagcatacat tcattcaaga gactttaaac aagctagcag
     1741 aacgccctaa aacggacaca caactcgatt tgtttagcta tgatacaggc tgaaaataaa
     1801 acccgcacta tgccattaca tttatatcta tgatacgtgt ttgttttttc tttgctgttt
     1861 agcgaatgat tagcagaaat atacagagta agattttaat taattattag ggggagaagg
     1921 agagagtagc ccgaaaactt ttagttggct tggactgaac gaagtgaggg aaaggctact
     1981 aaaacgtcga ggggcagtga gagcgaagcg aacacttgat tttttaattt tctatctttt
     2041 ataggtcatt agagtatacc tatttgtcct aatatgattt tagcagtata attgacttgg
     2101 tgaataggtc atttaagttg ggcataatag gaggagtaaa atgaaaaaat ttatttatcg
     2161 agttttagaa aatgacgaag tggtggctat ttttaatgag caacaatatg cgcaagattt
     2221 tatcgcttac gaaaagacaa tttctgataa gcaatttgaa attgaaaaag tagatattgc
     2281 tgattggtta ttgcaaccga gagaatttta gaggttggtt gaaaatggct aaaattggtt
     2341 atgcacgtgt cagtagcaaa gaacagaact tagatcggca attacaagcg ttacagggcg
     2401 tttctaaggt cttttcagac aaattaagcg gtcaatcggt cgaacgccca caattacaag
     2461 ctatgcttaa ctatattcgt gaaggggata ttgttattgt tactgaatta gatcgattag
     2521 gacgaaataa taaagaatta acagaattga tgaatcaaat tcaaattaag ggggcaaccc
     2581 tggaagtctt aaatttaccc tcaatgaatg gtattgaaga tgaaaattta aggcgtttga
     2641 ttaatagcct tgtcattgaa ttgtacaagt atcaagcaga atcagaacga aaaaaaatta
     2701 aggaacgtca ggcacaagga atcgaaattg ctaagaaaaa aggcaaattc aaaggtcgtc
     2761 agcataaatt taaagaaaat gatccacgtt taaagcatgc tttcgatttg tttttgaatg
     2821 gtttatccga taaagaagtt gaagaacaaa ctggaatcaa tcgccgaacg tttagaaggt
     2881 atcgagcaag atataacgtg acagtcgatc aaagaaaaaa caatgaaaag agggatagtt
     2941 aatgagtacg gttattttag ctgaaaaacc aagccaggca ttagcctacg caagtgcttt
     3001 aaaacaaagc accaaaaaag acggttattt tgagatcaaa gacccactat ttacagatga
     3061 aacgtttatc acctttggtt ttgggcattt agtggaatta gcagaaccag gtcattatga
     3121 cgaaaagtgg caaaattgga aacttgaatc tttgccgatt tttcctgatc gatacgattt
     3181 tgaagttgca aaagataagg gaaagcagtt taaaattgtt gcagaacttc tcaaaaaggc
     3241 aaatacaatt attgttgcaa cagatagcga cagagaaggt gaaaatatcg cctggtcgat
     3301 tatccataaa gcaaatgcct tttcaaaaga taaaacattt aaaagactat ggatcaatag
     3361 cttagaaaaa gatgtaatcc gaagcggttt tcaaaatttg caacctggaa tgaattacta
     3421 tcccttttat caagaagcgc aaacacgcca aattgccgat tggttgatcg gcatgaacgc
     3481 aagccctttg tatacgttaa atttacaaca gaagggcgta caaggtacat tttcactagg
     3541 acgtgttcaa acgcccacct tataccttat ttttcagcgc caggaagcca tagagaattt
     3601 taaaaaagaa ccttttttcg aggtggaagc tagtataaaa gtaaaccaag ggtcgtttaa
     3661 gggcgttcta agccccacac agcgttttaa aacccaagag gagcttttag cttttgtttc
     3721 ttctaaacaa gctaaaatag gcaatcaaga ggggataatt gctgatgttc aaaccaaaga
     3781 gaagaaaacg aatagtccga gtttgttttc tttaagtagt ttgcaatcaa aagtcaatca
     3841 gctttataaa gcgacagcga gccaaacttt aaaagctatt tcttttttaa taacttaaaa
     3901 ataaacttaa tgtaacagca agcacagtca aggtatacac ctttgacaaa aaatagcaca
     3961 ttctctatcg aaaatttttg cttatttttt aaattatttt gggaaatttt cccaatccct
     4021 ttttctaact caaaaaatat aatcactcaa aatttaaaag ggcgcactta tacatcattt
     4081 taaaaaattg atgtaacgtg ctaagttcaa aacaaagggc gcacttatac acgattttca
     4141 atcttgtata tttctaacga aaagcgtgcg ccaaaaaacc cccttcgtca attttgacag
     4201 ggggcttttt gatgtaaaaa tttctatcga aatttaaaaa ttcgcttcac tcatgttata
     4261 aagacttaaa ataaaataac tctttaaaat cttttgctag ttgttcttca atatttttta
     4321 ttcggtgcat cttccaagta aagtataaca cactagactt atttactacg tttcataagt
     4381 cattaatgcg tgtgctctgc gaggctagtt tttgtgcaag cacaaaaaat ggactgaata
     4441 aatcagtcca taagttcaaa accaaattca aaatcaaaac cacaagcaac caaaaaatgt
     4501 ggttgttata cgttcataaa ttttatgatc acttacgtgt ataaaattaa attcactttc
     4561 aaaatctaaa aactaaatcc aatcatctac cctatgaatt atatcttgaa attcattcat
     4621 aaatagtgaa gcatggtaac catcacatac agaatgatga agttgcagag caactggtat
     4681 ataaatttta ttattctcac tataaaattt acctatcgta ataataggca ataaaaagct
     4741 gctattgtta ccaatattta aattaaatga actaaaatca atccaaggaa tcattgaaat
     4801 cggtatggtg ttttcaggta tcggtttttt aggaaacatt tcttcttt
//
LOCUS       DAZKLA010000161         4840 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000161 DAZKLA010000000
VERSION     DAZKLA010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.165"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(235..597)
                     /locus_tag="ACG85N_002638"
     CDS             complement(235..597)
                     /locus_tag="ACG85N_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225346.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(671..1051)
                     /locus_tag="ACG85N_002639"
     CDS             complement(671..1051)
                     /locus_tag="ACG85N_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFQ0225347.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1133..2308
                     /locus_tag="ACG85N_002640"
     CDS             1133..2308
                     /locus_tag="ACG85N_002640"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFQ0225348.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2385..2969)
                     /locus_tag="ACG85N_002641"
     CDS             complement(2385..2969)
                     /locus_tag="ACG85N_002641"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0225349.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3170..4168
                     /locus_tag="ACG85N_002642"
     CDS             3170..4168
                     /locus_tag="ACG85N_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0225350.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4280..4789
                     /locus_tag="ACG85N_002643"
     CDS             4280..4789
                     /locus_tag="ACG85N_002643"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFQ0225351.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
BASE COUNT         1535 a          906 c          846 g         1553 t
ORIGIN      
        1 gttaaattta tcatgtattt cttccttggt caactataaa ttaagcatta ctttgtgaaa
       61 tgatttttcg atcaattatt attattcgtt atacaaccgt tttcaacata ttttgaaaat
      121 aaaatgcact aaaaaactat ctttagcaga acatttcatt gtattttttg gaccaaacaa
      181 taaaaaaaga agtttgaaaa aaattcaaac ttctttgttc gcttaatctg tattttagaa
      241 gaaaggcact ccctctactt cgtaagttcc aagaacagag cgcatttgac ctggttcttc
      301 ccaaatatat ccttcgccta attcttctct ttgattcttt tgttcagttt caaagacaaa
      361 caacggaaca ttttcctctt taatatgtgt atttgtcaat tccatcaaac taatcttgct
      421 aacatcgata tgaactattt cttttaagaa attaagtata ttcgctaaac cagtctttcc
      481 ttcagaaagt tccgctaacg caaattccaa cttgtcatca attgaatgga ccaaaaattt
      541 tttagtacca tcctccaaat gcaacataat tgtaccagca actcttttgc ttgccaaaat
      601 ttccacctcg atcaacttgt tctatggctt acataaaaat tctagcacat tttcagaaaa
      661 aatacacttt ttatttctta ttttctttcc gttctagttc agataatgct tcttctatcc
      721 aaataggtaa ctgctccgca atagtggtaa atccgatctt acgattttta ttcgtgaaat
      781 acctttttct gcgatatgga acagctggat ttgttttttc taacgttctt aaagataaac
      841 tgattttttt ggaatattca tcaatgtcga tgatctgaac tgttactggc tgtccgactt
      901 ttaacaatga atggatattt ttcgtataac cggattgtac ttctgaaaca tgaataagtc
      961 cttgcgtctc gttatcaaga gaaacgaatg cgccataagg ctgtatccct gtcacttttc
     1021 cttcaattat ttgtccaatt ttataagtca tctacttccc tctcttcgca tttatcatta
     1081 tactgtaaat agctcaaaaa aagaagcgac gaaaatgact ggaggtctta agatgatcga
     1141 atttgataaa cagatttcaa gaaaagatac agaaagtgta aaatgggacg caattgctga
     1201 gacatatcaa atggatgact tacttccttt gtgggtagca gatatggact tcttgtctcc
     1261 cggtggggta gctaaagcgt ttagtgaata catcaaacat ggaatctttg gttatgccac
     1321 tctgtctgat aaactatatc aggctatcat tcattgggaa cgccaacacc atgctgtgga
     1381 gttgacgaaa gaaaatatcg tttttacaag tggtgtgtta accagtttag ctgcagcagt
     1441 acaaactttt acaaaaccag gcgattctat tttgattcat gacccagttt atcctccatt
     1501 ttcctctatt atagaaacaa atcaacgtca catcgttcga agctctttgc tagaaaaaaa
     1561 taatcatttc atcatggact tgtctgacat ggagaaaaaa atcgaagaaa acaatgtaaa
     1621 agcaatgatt ttatgcaatc ctcacaatcc tggagggcgt gtttggagca aagaagaatt
     1681 gaaacagtta agcgaattgt gtttaaaaca tcaagtgatt ctgtttagtg acgagatcca
     1741 tcaagattta actttattcg atcatacatt tacttccatg ctaacgattg atcctaattt
     1801 agatgatcta ctagttgctt ttacttccgc aaccaagact ttcaatctag ctgcaatcaa
     1861 aaattcaatg gtatttatca aaaatccaga gatgaaagct gattttgaaa aacatttagt
     1921 gatgaaccaa cagcacgaaa tcaacacttt tggactgatt ggaacccaag cggcttatga
     1981 gacgggtgga gaatggttaa gccaactgat tccttatcta gaaaaaaatg ttcaaacagt
     2041 taatcagttt tttgaacagt atcttccgaa agtgagaata atgaaccctg aaggaactta
     2101 cttaatgtgg ttagatttta ccgcctatat ttcagacgac caagaattgg agaacactct
     2161 tgttcggaaa ggaaaagtcg tattaaatcc tggcatcacg tttggtcctt ccggtcatgg
     2221 gcatatgcgc ttgaatattg cttgtccaga agaaacttta ctggaaggac ttaatcgaat
     2281 caagtatgct ttagagacaa tcgattaaat catttattca aaataaaagg acgagacctt
     2341 actaggtttc gtcctttttc ataattactc cgtaccatac caaactattc tgaaatttca
     2401 attgtttcaa tcactacatc atgtacaggt cggtcttgtg gaccacgttg tacatttgcg
     2461 atttcatcta ctgtgtccat cccttctagt acatgtccaa agactgtatg tcggaaatct
     2521 agccaaggag ttccgccacc tttatacgct tcaatgattt ctgatgggaa tccagcacct
     2581 tctagttgtc caagcatatt agctggcaca tttgtgttat ttacaataaa gaattggctt
     2641 ccgtttgtgt ttggacctga atttgccata gataaagctc cgcgaaggtt gaacagttca
     2701 cgactgaatt catcttcaaa actttcgcca taaatacttt cgccacccat tcctgttcca
     2761 gttggatcgc cgccttggat catgaaatca ggaataacgc ggtgaaaaat cacgccatca
     2821 taataacctt ttttggaaag ttcaatgaaa ttttgaaccg tcttcggtgc tagttcaggg
     2881 aataattgaa cagtaatgtc accacggttt gttttgataa cagcttttgg tcctttttca
     2941 ctttttaaat ctagttgtgg gaatgccata ttgtttcctc ctaatatttg catacttcat
     3001 aataataata acataatacc taacctctcg taaaaatact agatttgttt tcttttattt
     3061 ttcatcacta gatacattct ataacttacc gtttcgattt cgaattcaaa catttttgag
     3121 aagcattctc aattaaattc tgctatagtg tgaaaaggga ggaattttta tggaaatcta
     3181 tgacattaca atcatcggtg caggtcctgt agggatgttt gccgcttttt acgcaggaat
     3241 gcgccaagct aaaacaaaaa tcattgacag tctacctcaa cttggtggtc aattagcaac
     3301 tttgtatccc gaaaaatata tttacgatat tcctgggtat ccagcaataa aagcaagtga
     3361 attaatcgat caattagaaa aacaattaac tacttttaac catacattcc atttaaaaga
     3421 agaagttctc tcgcttactc gtgaagacga agttatcgag attactacaa ataaaggcat
     3481 ccattattcc aaagcagtca ttttagcttt aggaaatggt tcattccagc caagaaaatt
     3541 gaatttagat aatgctgaat catttgaaaa ccacggattg gattatttcg tgaatgattt
     3601 gatgaagtat gctggaaaaa aagtcgctat tgctggaggt ggtgattctg ctatcgattg
     3661 gacgttgatg ttagagccta ttgcaagcga agtttacttg attcatcgtc gcccggaatt
     3721 tcgtgcgcac gaacatagtg tatcacgctt gaaatcttca tctgtcaact tgcttactcc
     3781 ctatctcatc gatggattat caggcaataa tggggaactg acagatatcc ggttaaaaaa
     3841 agtgaaaagt gacgagacga ttgatttgat gattgattct ctgatcgtca attatggatt
     3901 ctcttctaat ttagaacatc tttcttcttg gggacttgat agtaccagaa atgccattac
     3961 tgtaaaatca gatatgtcta cttctattcc aggcgtttat gcagctggtg acatctgtac
     4021 ctatgaagga aaagtgaaac tgattgctac tgggctcggt gaagcaccta ctgcagtgaa
     4081 caacgcttta cactatatta atccaaagga acgaactcag cctggtcaca gcacaagctt
     4141 atacgataaa aatcttcgtc ctagttgatt ttttatatct ctttgcattt caagttaaac
     4201 ccgaaagcag tttcttgcat ttttttcatg ttccgttaac atgaaataga gaaagaaaaa
     4261 ataaaggggg agcaataata tggtcgtcaa aactgagact ttagaagaaa aggcaagaga
     4321 actattgaag gaacgtggcg taactgtaga agatatcgca caacttgtca tgtttttaca
     4381 aaaagactat atttctgatt tgactttggc ggattgtact gaaagtgtca acagcgtgct
     4441 aacaaagcgt gaagtacata acgccattat cactggaatc caattagata tattagcaga
     4501 aaagaaaaaa ttgatgagtc cgttgcaaca tattatcgaa gatgacgaag gcctttacgg
     4561 tatcgatgag atcctagcct tgtcaatcgt gaatgtttac ggtacgatcg gctttacgaa
     4621 ttatggttat atcgacaagg tgaaacctgg tattttaaaa gaactaaaca gccacgatgg
     4681 cgttcatgtc cataccttct tagatgatat cgtcggagcg atcgccgctg ctgctgccag
     4741 tcgattagca cacaaagaac cagaaaaaag aagtcgcctt actcaataat tcaacaaaaa
     4801 aagaaagaaa acgtctattt agacgagttc tttctttttt
//
LOCUS       DAZKLA010000162         4797 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000162 DAZKLA010000000
VERSION     DAZKLA010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.166"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG85N_002644"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG85N_002644"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFQ0225352.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACG85N_002645"
     CDS             complement(1297..2133)
                     /locus_tag="ACG85N_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0225353.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACG85N_002646"
     CDS             complement(2317..3534)
                     /locus_tag="ACG85N_002646"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0225354.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACG85N_002647"
     CDS             complement(3683..3844)
                     /locus_tag="ACG85N_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225355.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACG85N_002648"
     tRNA            3917..4000
                     /locus_tag="ACG85N_002648"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..>4797)
                     /locus_tag="ACG85N_002649"
     CDS             complement(4240..>4797)
                     /locus_tag="ACG85N_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225356.1"
                     /translation="LFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTY
                     VNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNK
                     KTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSV
                     QKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1455 a         1001 c          774 g         1567 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatag
//
LOCUS       DAZKLA010000163         4793 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000163 DAZKLA010000000
VERSION     DAZKLA010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.167"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..288
                     /locus_tag="ACG85N_002650"
     CDS             <1..288
                     /locus_tag="ACG85N_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225357.1"
                     /translation="LKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIHRF
                     IRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            347..787
                     /locus_tag="ACG85N_002651"
     CDS             347..787
                     /locus_tag="ACG85N_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225358.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            799..1467
                     /locus_tag="ACG85N_002652"
     CDS             799..1467
                     /locus_tag="ACG85N_002652"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0225359.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1531..2154
                     /locus_tag="ACG85N_002653"
     CDS             1531..2154
                     /locus_tag="ACG85N_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFQ0225360.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2210..2596
                     /locus_tag="ACG85N_002654"
     CDS             2210..2596
                     /locus_tag="ACG85N_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFQ0225361.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            2809..4200
                     /locus_tag="ACG85N_002655"
     CDS             2809..4200
                     /locus_tag="ACG85N_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFQ0225362.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1591 a          802 c         1009 g         1391 t
ORIGIN      
        1 ctaaaaagct ttttaaaagt gatcagtaaa aaacggaatc tgatcaaaac gacacagctg
       61 ttgaaaaaat atccaggtct gcttcacacg tctattcaat ttcctcaatt gattacgatg
      121 atccatcgtt ttatacgtat gcaacagcta ccatctaaga agctgtttct tccattgacg
      181 gacacatttc gagtagtcaa cagagaagat aaaaagaaag tgattgtgat tgactttacc
      241 agaaatcgga atgtccttca ccaagttgca atggaaaagg ccaattaagg ctttttcttt
      301 ttggattttt ttgtcataat aaatattaga tgatggagga gataatatga tgcaaacatg
      361 gttagaaagg ctaatacgat ttcagcagaa ctatcacaaa cttatgcagg gaagatatgc
      421 gaaatttgat cagttgaatc gcggtctatt gatcgctgcg cttgtcttta gtttgctaag
      481 tcgttggttg ccctatagaa tcggacaatt gttgttcatc gtctttttcg gatggatgat
      541 atttcgtttc ctatcaaaaa aaatctaccc cagactaaat gagaatcaac gttatttgaa
      601 atatcttgat agagtcaaag aaaaatatca tcagataaaa aacaaaaagc aagtcaaaat
      661 ggatcgaaaa agttatactt tttttgagtg cccgaattgt catcaaaagc agcgtgcacc
      721 taaaggaaaa ggacgaatac gtgtgacatg caaaacatgt ggtataaaat tcgaaacgaa
      781 tgtttaaggg agaaaagaat gaaattagta ttattacgtc atggcgaaag cgaagcaaat
      841 tttgaaaatt actggacagg atggctagat gtcgccttaa cagaaaaagg aaaagaacaa
      901 gcagcagttg ccggtcaaaa aatgcttgaa gccggacttc aattcgatgc cgtttatacc
      961 tctgttttga aacgtgcgat tcttaccgga caagtcgcac tagaagaaat gggtcagtta
     1021 tggcttccta taataaaaac atggcgtcta aatgaacggc attatgggga tcttgtgggt
     1081 aaaaacaaag ataagatgaa agaaatatac ggaaaagatc aagtaaaaac atggcgcaga
     1141 ggatatacgg aagttccgcc gttaaccgca gaaaatagat ttgaccgcag atatgatcac
     1201 ttggatccgc atttgatccc atacggagaa agtctggaaa tgacagtgaa tcggatcatt
     1261 cccttatggc aagatcatct agcaccaaaa ctaaaagatc ggcaagattt actagttgtg
     1321 ggtcatggta atagcatccg agcactgaca aaatatttag aagatgttcc tgaagatcaa
     1381 atggatacga tcgatatccc aaatgcacaa ccaatccagt atacattagc agatgatttg
     1441 acaattctgg acaaaaaaat actttaaaga acacctttat ctaaaagtga gagatagaaa
     1501 aaagaagcaa aggaatggaa caaaaggaac atgaaaaatt atatatggga ctttgatggt
     1561 acattgtttg atacgtatcc cgcaatggta gatggtgcat ggcaagcact aaaagatttt
     1621 ggtatcagta tggacaaaaa agaaatctac ttcaaaatga aaaagtattc tactagttat
     1681 ttgatcaatg aatcaaactt gaatgccaga gagtttaatg aattatttca ccgatatgaa
     1741 aaagaaagta cggaagtttc ccgtccattt ccagaaacga aacaagtact ggaaatgcta
     1801 aaagacaacg gcggtcgtca tttcatcctg acccaccgtt tgacagaatc aacatggggc
     1861 ctgttaaaag aacatcgatt ggcacatctg attgaggaag tagtagggat cgatcaggac
     1921 tttcctcgta aacctgatcc agcatcttta aattatttga tcgatacttt tcatttagaa
     1981 agaacagaca cgatgatgat cggcgatcgt cgtttagata tcgaagcagg gaaaaatgca
     2041 ggagtcgcta cttgtcttta cgacatcgat cattttctgg gtgagatccc ggcagattat
     2101 gtggtaggaa atctgaatga aatccttacc ttgcagagca tcattaaaaa gtagtatccc
     2161 ctttcaaagg agatatgtta taattttcat aaaggagtga taaaattata tggaaatcag
     2221 aagacaaaaa gaagttgtag aagcatttca ttatgatatg agattgccga accaagaagt
     2281 agaaacagat ttgaaagtag gcttctcgcc tttaaaagaa acgatcgaaa actatccgaa
     2341 agaacatagc atcgtaggag cacgcttaga gttccgttta gtgtttgata catatgtttt
     2401 atctggaagt gtgagtcaga tcaatcatat cctcaatcgg aaaattgaag gtcaaaatga
     2461 tgtcacacaa gaagaagtgg acgagctagt agcaccgtta ttcgatatcg tacgccgaat
     2521 gtcttatgag gtgacagaga tcgccttaga tcagcctggg gtaaaattga attttcaatc
     2581 agaaaaacaa gaatagacag taaaaacata cctgcgatag attaaagcta tcacaggtat
     2641 gtttttttat tactaattgt aagcaaaaag aatgtcattt cgataaaaat cgtctaaaat
     2701 ataaaagtca ctgaaaaaaa ttttttcagg aggatggttg aagaatcatt ctcgggaacc
     2761 tttacttcct tcgatggact tgtcattaat aatatgagga ggagaaaaat gacaacactc
     2821 atcacagttt tagtggttct agtttttgcg gcattattat atgtgttcta tcgtatgcaa
     2881 aagcgccata caaaattttc aacacgcgtc tttacagcat taggagttgg tatcgtcttt
     2941 ggcggtctgc ttcaattgat ctttggtgta gacgacaaag tcgttactca atcaatggaa
     3001 tggatcggga ttgtaggtca aggatatgta tcattcttgc aaatgctagt tatgccttta
     3061 gtgtttattt cgatcattgg tgcattcaca aaaatgaaag tttctgaaaa gctagggaaa
     3121 attagtgcga ctgtattgac ttctttactt ggaacaacag ctattgcagc attgatcgga
     3181 attggttcaa caatgctgtt cggactccaa ggagctaaat tcacacaagg agctgcagaa
     3241 acagcacgaa tctctgaact ggcaacacgt caggaaacag ttgaaaactt atcgattcca
     3301 cagcagattt tatcctttat ccctaaaaac gtttttgctg atttagcagg tagtcgtgca
     3361 acaagtacga ttggagtcgt tattttcgcg gcatttgtcg gaatcgctta tctaggcgtg
     3421 aaaagaaaag cgccaaaaga agcagcattc ttttcagatt tagtcgacag tctgtataaa
     3481 atcattatgc ggatcgtgac gcttgttttg cgtttgacac cttatggagt atttgctttg
     3541 atgacaaacg ttttggcaac aagtgatttt gcagctatcg tcaatttagg taaatttgtc
     3601 gttgcttctt acgtagcatt gcttgctgtt ttgattgtcc actcactgat tttgattggt
     3661 gcaaaagtca atccaatcaa ctatttcaaa aaagcctttc cggtattgag ctttgcgttt
     3721 acttcacgtt caagtgctgg agctttgcca ttgaatatcg agactcagac aaaagcttta
     3781 ggagtagatg atgcaacagc taatttctca gcaagttttg gtttgtctat tggacaaaac
     3841 ggctgtgctg gcgtttatcc agctatgttg gcaacgatcg tcgcaccagc agctggagtg
     3901 aatgtcttta gtttggaatt tattttgatg cttgtggcaa ttgtaacaat cagttctttt
     3961 ggtgtagctg gagtcggtgg gggcgcaaca tttgcttcac tgatcgtttt aggggcaatg
     4021 aacttgccag tagcaatcgt tgggttagtt atctctgttg aaccattaat cgatatggca
     4081 cgtacagcag taaacgtcag cggtagcatg gtagctggta tcgtgacaag tgcacgcatc
     4141 aaagatttag atcgcgatgt catcaatgat gattctaaag tgatcgatgt gaatgcatag
     4201 tcaaaaagag gttgtgatag aagtggtcag ctttaagaaa taagaagaag tttcctaaaa
     4261 atagtttctc aaatttttgt gaaatttcgg cttatttccg agaagctact tctgaaacac
     4321 cgtgaataag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaaacaaa
     4381 aatagttcct catattttac gtttggttga gcttattacc gcagatgcaa gcttttgaac
     4441 accgtttaag aaagaggtcg tgagcctgcc gtttcactct gagtcacaaa gagcaatttc
     4501 aaaaaatagc ttctcatgtt tgttgaatat tgtgtcttgt gaccgaggag ttgtcaggag
     4561 aacaccgttt accagaggtt gtgacttgtt tttagtcacg acctcttttt ttatttattc
     4621 catataaata cacattattt attccgaaaa gtgaaagcgc aaataacaaa aacaaccaac
     4681 ttatttatta ttaaagaaaa gcgtatttaa gtaggacgat tctgttgaga gctttcgtag
     4741 attttcccat tttcctatgg taaactgaca aagttgagaa tgttagaaca gat
//
LOCUS       DAZKLA010000164         4757 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000164 DAZKLA010000000
VERSION     DAZKLA010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.168"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..746
                     /gene="vanR-A"
                     /locus_tag="ACG85N_002656"
     CDS             51..746
                     /gene="vanR-A"
                     /locus_tag="ACG85N_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0225363.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="ACG85N_002657"
     CDS             724..1878
                     /locus_tag="ACG85N_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0225364.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="ACG85N_002658"
     CDS             2093..3061
                     /locus_tag="ACG85N_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0225365.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="ACG85N_002659"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="ACG85N_002659"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0225366.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="ACG85N_002660"
     CDS             4091..4699
                     /locus_tag="ACG85N_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0225367.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1471 a          910 c         1115 g         1261 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaacggttct gttgcaaagt
     4741 tttaaataaa gaataaa
//
LOCUS       DAZKLA010000165         4695 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000165 DAZKLA010000000
VERSION     DAZKLA010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.169"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..1098)
                     /locus_tag="ACG85N_002661"
     CDS             complement(136..1098)
                     /locus_tag="ACG85N_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0225368.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1100..2539)
                     /locus_tag="ACG85N_002662"
     CDS             complement(1100..2539)
                     /locus_tag="ACG85N_002662"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFQ0225369.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2724..4670
                     /locus_tag="ACG85N_002663"
     CDS             2724..4670
                     /locus_tag="ACG85N_002663"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFQ0225370.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1486 a          807 c          776 g         1626 t
ORIGIN      
        1 tttctccact ccaaaaaagt ctaacttttg ggggtcacta caagttgaca actccttttt
       61 gtttacaaat caattggata tctacaaatt gtattttcta acgaatttta tagacattaa
      121 aaaattaatt taacgttaaa tacttcctcc agaaagtatt gagattggta aaatataatc
      181 cttatttgtc ttttcgcctt taattttttt cagaagaata tcaacaataa gacttgcaat
      241 ttcatcaatt ggttgtctta ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt
      301 accatcgtat ccaataattt ttaaatcttc tgggatatta agtttaagtt gatgagcaat
      361 tttcatagtg agaatggctg ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt
      421 tataatagtt gactttattt ccatttcacg acggatagta gacagattat taggaatttt
      481 gaaaattttt ccatttggat tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga
      541 atccgtgtta tcatttcctg taatcataat ggtattttga caaccatttt tttgtaaagt
      601 cttagctgca agttttcccc cttcaaagtt atctgaagaa acaatggaaa cattaggagc
      661 taaatttcta tcaaaagcaa caataggtgc tctcacacgc tcataatcag caattccaag
      721 attatggctt gatgagatta tgccatcaac ttggttggct tcaagcattt caatgtattc
      781 tctctcttta gcaggatcat tttgactatt gcaaataatc gttttatatc catgtttaaa
      841 taactctatt tcgagatact caatgagttc agagtaaaag atattactaa tattaggaaa
      901 aataagacca ataagctgag gtgactttcc ttgaagtcca cgtgctaaat tatttggttt
      961 ataaccaaga gttttcatag cttcatgaac tttttgtttt gttttttctg ataaatatcc
     1021 cttattatta atgacgcgtg aaacggttgt tgggctaact ccagctaatt cagcgacatc
     1081 agttaatttt gctaccattt taatatttta attcaaagta ttttccagta ggttcgcctg
     1141 atttgatttg aataccattt tggtgatttt cagggaatac acgactagta aacacttttt
     1201 ctcctttatt aataaaaatc tcaatgattg agttatctac aaaaatgtta gcagttgttt
     1261 ctttagtatc aattgagcat tcacgaatcg taccaaagtc agtggcatat tgaatgcctg
     1321 ctttgctgcg atctaaaata atttttcctt ctttggtatc tacaatcaag ctaagtccat
     1381 ttcctttact atcagagaaa agaagtaact ccgatttctg atttggagga aaagttagtt
     1441 caagctcata agtattattt gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta
     1501 atgatgtaat agcttctact ggatattggt aaagtttacc atcttttatt gaaagttctt
     1561 ttacaagact cattgctccc tgataatcat acttatcagt tggataatcg atatcaggta
     1621 gtccaatcca actgacaatc agagcacgac catcaggagt attgaatcct tgagtcgcat
     1681 acgcttcaaa tccataatca agattttgaa tttctgaagc gcccacaagt tttacttttt
     1741 ctatgtcaag cgattgacag atcttaaaag tattcggata gatattccca taatttagct
     1801 cagatttatc caacccttga ggacaataaa tgagaacagg ttttttgtct acaaatacta
     1861 gatttggaca ctcaatcatg tattctgaac cagtgccgcc aaaatcaagg ttgccaactt
     1921 cctcccatgt ttccacgtta ttgtcatccg ctttgtaaag tttgataaag cctgattttt
     1981 ctaaattttg tgctccaaca atcgcataca attgtccttc ataattaaac atttgtggat
     2041 cacgaaaatg atcagttaca tcagcaggtt tatgaataag aacattctca cctttcgtaa
     2101 tgttgtagtc tttatccatc catgctccga tttgaagtgt atcacgttcc caattttcat
     2161 ttcgaacgtt tcctgtatag agtaagaata gattcccctc tatttcatat gcagaaccgg
     2221 aataagcgcc gtggctatct cgttttgtgt ctggaagaag ttctgctcct gttacatgaa
     2281 aatgaactaa atcatctgat tcattgtgta cccattcttt caacccatga gcagctccaa
     2341 atggccaatt ttgataaaaa agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat
     2401 cgtttaacaa tcctgtttta ggttcgatat gatagctagc acgccaaggt gatcgtttta
     2461 cattatcctt aattttatcc atctcatctt tagtccaatc agaataagca cgatatcgta
     2521 tttctcgagg taagttcata ttttactcct tcaatttatc tttttacaac tagtatggta
     2581 aacgttttcc gttgaaaaat caatcatttc atttaaaaaa ataaactata tgacaaacgt
     2641 ttgacatatt tttaagatat gctaaaataa atttgcattt cagtgaaccg ttttcacgta
     2701 aaaataaaaa aggagtgatt cgaatgaatt acagtaaagt agcttccgac gtaatacaag
     2761 cggtcggaaa agataacctt attgcagctg cacactgcgc tacacgtctt cgtctcgtac
     2821 taaaagatga cacgaaagtg aatcaaaaag ctttagacga aaatccagat gtgaaaggca
     2881 catttaaaat tgatggacaa tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt
     2941 atgacgaact tatcaaaaaa actggacttt cggaaatttc aacagatgat ttaaaacaaa
     3001 ttgctagtaa agataaaaaa ttcaacccca ttatggcatt aattaaatta ttatcagata
     3061 tttttgttcc aattatacct gcccttgttg caggtggttt attaatggca ttacaaaact
     3121 ttttaacagc tgctggactt tttggaccaa aatcaattga agaaatgtat cctgcgattg
     3181 aaggtatttc ctcaatgatc caactgatgt ccgctgcacc attcatgttc ttaccaattt
     3241 tagtaggtat ttcagctgct aaacgttttg gagcaaacca atttcttggt gctgcaattg
     3301 gtatgatcat gacaacacca gatcttggtg gctcaactga atattggaat atttttggtt
     3361 atcatgtttc acaaacaaac tatgcttatc aagttattcc agttttagca gctgtttgga
     3421 tattatctat attagaaaaa ttctttcata aaaaattgcc atcatctatt gactttacat
     3481 tcacgccatt attatcagta atgattactg gatttttgac tttcacagtt attggccctg
     3541 taatgttatt gttgtctaat ggaattacag acattattgt ttggctatat aacactactg
     3601 gatttattgg aatgggtatt tttggaggaa catattcact tatagtcatg actggtttac
     3661 atcaatcatt tccagctatt gaaactcaat tactttcagc gtggacaaat ggtatcggac
     3721 atggagactt tatctttgta gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat
     3781 ttgccatttg gttcttaact aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg
     3841 tctcagcact tcttggaatt acagagcctg ccttgttcgg agttaactta aaataccgct
     3901 ttccattctt ttgtgcttta attggttcag gaattgctac agcagtagca ggattactta
     3961 aagtagttgc tgtttcactt ggttcagcag gtttcttggg atttctttca attaatgcaa
     4021 catctattcc attttacgta ttgtgtgaat tgattagctt tgtagttgct tttgcaatta
     4081 cttacttcta cggtaaaaca aaagctgctg gtatctttaa agcagaagaa attgctgaac
     4141 aaacaccagt taattcttca aaagtagcaa caacagatcc aatatctact gatgttgaat
     4201 caacacaaac aaaagtttca tcagaaacaa ttgttagtcc catttctggt aaagtgatta
     4261 gtctttcttc agtaaatgat ccagtctttt catcagaagc tatgggaaaa gggattgcca
     4321 ttaaaccaaa tggaaacacg gtccattctc cagtagatgg tactattcaa ctcacgtttg
     4381 aaactggaca tgcttatggt attaaatcaa ataatggtgc tgaagttcta attcatattg
     4441 gtattgatac tgtatcaatg ggaggaaaag gatttacaca aaaagttact actaatcaaa
     4501 aagttagaaa aggcgatatt cttggagcat ttgaccgttc aatgattgag aaagaaggtc
     4561 ttgatgatac aacaatgatt attgtgacca atacttcgga ttattcagaa gtaaatatag
     4621 tagctgaaaa tacagtcact gagggtactg atttacttga aatcaaataa atatatttcc
     4681 ctgagtaaag atgga
//
LOCUS       DAZKLA010000166         4660 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000166 DAZKLA010000000
VERSION     DAZKLA010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.170"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(104..1336)
                     /locus_tag="ACG85N_002664"
     CDS             complement(104..1336)
                     /locus_tag="ACG85N_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFQ0225371.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="ACG85N_002665"
     CDS             1591..2160
                     /locus_tag="ACG85N_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0225372.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="ACG85N_002666"
     CDS             complement(2338..2778)
                     /locus_tag="ACG85N_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFQ0225373.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="ACG85N_002667"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="ACG85N_002667"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFQ0225374.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="ACG85N_002668"
     CDS             3732..4655
                     /locus_tag="ACG85N_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFQ0225375.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
BASE COUNT         1410 a          865 c          923 g         1462 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca
//
LOCUS       DAZKLA010000167         4586 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000167 DAZKLA010000000
VERSION     DAZKLA010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.171"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..315)
                     /locus_tag="ACG85N_002669"
     CDS             complement(85..315)
                     /locus_tag="ACG85N_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFQ0225376.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(545..1363)
                     /locus_tag="ACG85N_002670"
     CDS             complement(545..1363)
                     /locus_tag="ACG85N_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0225377.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1524..2213
                     /locus_tag="ACG85N_002671"
     CDS             1524..2213
                     /locus_tag="ACG85N_002671"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0225378.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2227..3729
                     /locus_tag="ACG85N_002672"
     CDS             2227..3729
                     /locus_tag="ACG85N_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0225379.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3742..4218
                     /locus_tag="ACG85N_002673"
     CDS             3742..4218
                     /locus_tag="ACG85N_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0225380.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(4315..4425)
                     /locus_tag="ACG85N_002674"
                     /pseudo
     CDS             complement(4315..4425)
                     /locus_tag="ACG85N_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1527 a          660 c          814 g         1585 t
ORIGIN      
        1 atgaaatggg tggaaattga ttcttataga cagtttgtct cataacattg aagtattttg
       61 caattttagc attgaaacac ttttttaatc ttcaacgttg ttcttataaa caagatgatt
      121 gggaatcttt tgaattaaca ctgtatagct tagaacaccc tttatagaaa acattcgctt
      181 agcaatctta ataattcctt gtgcccgatg tcgtttagat tcagtaggtt cctgctcatc
      241 gatcatggaa aaaatttgaa ggatcagatc cttaataaat ttatttaaga gaggatgatc
      301 cattgcttca tccatactcg ataaactagt gataatgagc tacacgtttt tctttcaagt
      361 tgatcaatag cttccacgat aaaaatatct ttagataaaa tcaagaaccg taggaactac
      421 agggatattt ggtcaataag agccaccgct attagtaaaa aataagctat caagtatgct
      481 ctcatcccaa gaattgccta cttcaatcgt taaaaaccgt taggtttagc taagtggggg
      541 tagttcactc aatactttta tcaataatag agcgcaaaac tttttctctt aattcaactg
      601 aatcaatatt attgttttgt tcataaccat gaactaatag atcacaaata taaagttgtg
      661 atatttttcc agctaaagat ccaccattaa gaaattcttc aatggctgtt tgtaaaacta
      721 agtctgcaga ctttcctata ggtgaatgaa tatagttagt tatagcaata atttttgcgc
      781 catttttttt agctattttc aatgaatcat aagtatcttt cgttttccca gataaagaaa
      841 aaatgattac taaatcatta actgttaata aagaagcgac ttgggcttga aaatgagggt
      901 ctagtacagc ttttgcttga actcctacgc gaagaaacat attttctaaa tcaagactag
      961 tatttccact agatcccact ccaaatatat atatgttttt tgattgagtg atcattttaa
     1021 tggcttcatt caatttttct tcgtctaaaa gcctaatagt ggaatagaga gcttctgtta
     1081 aactattagc aatttcatca tagtacttta cactagtcgg atgttctttt ttttgactaa
     1141 agtcttcctt tgcaatttca atttttaaat cagaaaaacc agaaaaacca attttttgac
     1201 aaaagcgaat aatagtagca tcgccaactt ttgtgttttt cttaatatca ctcatcgtac
     1261 tataaataat agttttccca gaatttaaaa taaaatcagc gacttttttt tcagacttcg
     1321 ttaaaaatgg ataattttct ttaattaaat agatataact catacaaact cctctacaat
     1381 ttcaactttt ctccattata ctccaaaata ataaaaaaaa ctcctaaata ctccattttt
     1441 atctttactt ttatgaaagc ggatgctata ttgtttttgg agttattatc caaaaataat
     1501 aaaaaattgg agcgtttagg ataatggata aaaagaggtt tttagatctt atttcaaaag
     1561 gtttgattgt ttcatgtcaa gcattacctg gtgaaccatt ttacaaagag gaagaaagtt
     1621 taatgcctta ttttgctttg gctgctcaaa gggctggagc agttggtata cgtgcaaatt
     1681 ctgttcgaga tattattgaa attagaaagt gtgtatcatt accaatgatt ggaattatta
     1741 aaagagatta tccaccagaa tctccattca ttactgccac tatgagagaa gtagatgaat
     1801 tagtagaaac cggtgttgag gtaatcgctc ttgattgcac tcttagaaat cgacatgatg
     1861 gactatctat aaattctttt attactaaaa taaaagaaaa atatcctcat cagttattta
     1921 tggcagatat ttctacattt gaagaaggca aaaatgcata taaagcagga atagattttg
     1981 taggaactac tttaagtgga tatactgagg aaagtaaaaa acaagatggg ccagatatag
     2041 atttgattaa aaaattggta gaagaaggga tttgtgtaat tgctgaggga aggattcata
     2101 ctccagaaca agcaaaagag ataaaaaaaa taggagttgc aggtattgtt gtgggtggtg
     2161 ctattactcg accacaggaa attgcatcaa gatttgttaa agcattaagt tagggggaga
     2221 aataaaatgt ttaaaaaatt ttcacaatta ggacgcgctt tcatgttacc aattgctata
     2281 ttaccagtag ccggattact attgggactg ggaggagcgt taacaaatga gagtgctata
     2341 aatgcttatc catttttaga tcaaccgtgg ttgcatacaa ttttaagtat tatgagttat
     2401 gctggaaatg cagtttttgc aaacctagca ctaatttttg caattggtat tgctgtagga
     2461 cttgcaaatg gagataaagg aacagctggt ttagctggtg gagtagctta tctagtatat
     2521 actgcaacta ttagtgggtt tcttgaactt ttttcagcaa aagatgcaac actagataca
     2581 ggtgtagtgg gatcaattgt tgttggtggt actgttgcat ttttacacaa tcgttataga
     2641 aaaattgaat taccacaatt cttaggtttt tttggaggat caagatttat tccgattgtt
     2701 tctagtttag ctgcaatcat tattggttct ttcttttatt taatttggcc accaattcaa
     2761 aatggtttag tagttgctgg tgaacaaatt gcccaaatgg gtagtttagg aactttttta
     2821 tatggttttt tattgcgttt aacaggtgct gttggactac accacaccat ttaccctttg
     2881 ttctggtata catctttagg tggtaccgaa actgtagctg gatcaacaat tgctggagcc
     2941 caaaatattt tctttgccca actagctgat ccaaaccaca ctggtctgtt tacttatggt
     3001 actagatttt ttgcaggaag atttgcaaca atgatgtttg gattaccagc agcatgttat
     3061 gcaatgtatc gtgcaattcc taagaaaaat cgtaaaaaga atggtgggct ttattttagc
     3121 ggggccctta catcattttt aactggaatt actgaaccag ttgaatatat gtttttattt
     3181 gttgctcctt ggctttatgt gattcatgca ttcttagatg gattatcttt ttattttgct
     3241 gatatcttaa atattcgtgt agggaattcc ttttcaggtg gtttgatcga ctttttatta
     3301 ttcggtgtac ttcaagggaa tgacaaaact aattggttaa aattaattcc ttttggtatt
     3361 gcatgggcat ttatatattt ctttgttttt acattcttta tcaaaaaatt taaggtagct
     3421 attcctggaa tggaagaaga atcagaagtt cattctgaaa aagtaataaa caaaaataca
     3481 tcattacatg aagagtctct tatcattatt gaagctcttg gtggaaaaga aaatattgag
     3541 tctgttgaag cttgtgcaac tcgtttgcgt gttgctgtta cgaatgggga gattattcaa
     3601 aaacaaacaa taaaagacac tggtgcaact gctgtcttcg aagtgaaggg tggcatccaa
     3661 gctgtttttg gagggaaagc tgacttatta agtcaagaaa tcaatcaaat attaggaaaa
     3721 gataattagg aggtttttaa tatgtttgaa attttcaaaa agaaaaaaca agaaactttt
     3781 tatgcacctt gttccggaaa aatcatttcc atcacagaag taaatgatcc tgtcttttca
     3841 caaaaattac taggtgatgg ttttgccgtc atacctgatg gttcaaaaat ttatgctcca
     3901 ttggctggtc gagtaacaaa tatatttcct actaaacatg ctattaatat aaaaagcaac
     3961 tcaggcattg attatttgat tcatattgga attgatacag ttgaattaca aggagagcct
     4021 ttttcgattt gtgttcaaga aaatgatgag gttactcctg aaacctcgtt ggttgatgtg
     4081 gattttgaca aaataataaa atctggtaaa gatacttctg ttatagttgt ttttccggag
     4141 aaaaatcagt tcaatgattt aataatagat aatggtcgaa ttgctcatgg gaattcatgt
     4201 ggaaaaatca ctttttaaaa aagtgtatat aaaaagctat aaaataagct aaataggata
     4261 tttgtcaaat gaagttggtg aacagaaaga actgttttct ttctgttcat ttttttattt
     4321 tattagtaat gattccccat gccaaatcat gatccgtaat ttaaagttat aaaagcttcg
     4381 aaatccattg gcatttcgtt ggaatacctt gatatgatta ttcaaatata aaatatagag
     4441 agaagagaga gagggaagat atttataaag ggcaaagtta tgtttactca acttaggaaa
     4501 tagaaataga tcaatttata ttctcttgtc aatcgaatat gaaccttagc actatatacc
     4561 aaatatagta aaatgctgaa agatgt
//
LOCUS       DAZKLA010000168         4560 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000168 DAZKLA010000000
VERSION     DAZKLA010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.172"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..792
                     /locus_tag="ACG85N_002675"
     CDS             <1..792
                     /locus_tag="ACG85N_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0225381.1"
                     /translation="FCGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKK
                     GQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSF
                     KGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEI
                     IRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEY
                     SIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            889..3138
                     /locus_tag="ACG85N_002676"
     CDS             889..3138
                     /locus_tag="ACG85N_002676"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0225382.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3141..4316
                     /locus_tag="ACG85N_002677"
     CDS             3141..4316
                     /locus_tag="ACG85N_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0225383.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRVTRGAS"
     gene            complement(4319..>4560)
                     /locus_tag="ACG85N_002678"
     CDS             complement(4319..>4560)
                     /locus_tag="ACG85N_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0225384.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1578 a          659 c          834 g         1489 t
ORIGIN      
        1 ttttgtgggt attcaaaaac agaacctaga catagttttg gaccagctat tcgagaacaa
       61 tacttaatac acatagtgct agaaggtgaa ggctattatt caattaatga tcaaaaatat
      121 tttctgaaga aaggccaagg atttgttatc ccaccaggag tatctacttt ttatcaagca
      181 gatgaaaaaa atccgtggtc atatgtatgg ttgggtttaa gtggcaatct tgtagaatac
      241 tatctaaaag aaattggtgt tggaaagaat catcttacct ttaaagtcga aaatttaaat
      301 tcatttaaag gattaatttt tgaaacttta tcgtatgatc aggacaatct agtcagtgaa
      361 ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc agctagttat
      421 ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac aattgaaata
      481 atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag tctatcgata
      541 cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat aaaagagtat
      601 attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga atattcaatc
      661 cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa agcctttaag
      721 aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct aggaatcatt
      781 aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa acgcttacta
      841 tttatgtgag cgttttttat aatgcaataa agaaacggag gagagcttat gtataaggat
      901 aatgaaatga atattattta tcgatcagaa aaccaacaat ttcatttgtt taatcaggag
      961 ataagttaca tttttaagat atcggaagat ggtaaattat tgcatttata ttatggtgag
     1021 aacttaccag ataatgacta tagtcatcta attgagatgc atcatcggcc tatgacaacc
     1081 tatagaaatg aaaacgattt gctatattcg ttagagcact taaaacaaga atttcctgaa
     1141 tatggtacaa cagattttag acatcctgct atttcattac tacagaaaaa tggctctcgc
     1201 ttatcagatt ttgtctatca agggtacgaa gtggaagaag gaaaacctaa actagaaggt
     1261 ttacctgcta cctatgttga taataatgat gaaagcaaaa cattgaaagt ttatatttta
     1321 gattcagtta caaatgttca agtagaatta ttatatacaa tttatagaga ttatcctgtt
     1381 attactcgta gcgcgcgagt tatgaataaa ggaaaagata ttcaaataat cgagaatatt
     1441 agcagtttat cattggattt acccgatgca aattatgaat ggcaacaatt atctggtgct
     1501 tggggtagag aaagaactat taagtcgcgt actttacaac aaggaattca atcaatcgaa
     1561 tctactcgag gtatttcaag ccatcagcat aacccatttg ttactttaaa aagaaaaaat
     1621 actgatgaaa atcaaggaga agctattggt gcagcactag tttactcagg aaacttctta
     1681 attcaagcag aagtagatac atgggatgtt actagactac aagttggcat caatccattt
     1741 ggatttaatt ggaaactcaa tccaggagaa atatttacag caccagaagc gattctggtt
     1801 tattcaagta agggtttaaa tgccatgagt caaacttttc atcaactatt tagaaaacgc
     1861 ttagctcgtg gtaagtggcg agaaaaagac cgtcctgtct taattaataa ttgggaagca
     1921 acatattttg attttgacga agaaaagttg gtaaatattg ctaaaaaagc atttgatgtt
     1981 ggtattgaat tatttgtatt agatgatggc tggtttggag aaagagaaaa cgattgtgca
     2041 ggattaggag attggcatgt aaatcctaaa agactaccta gaggaatcgg cagcctttca
     2101 gaaaaaattc gtagttttgg tatgaaattt ggtttatggt ttgaacctga aatggtgaat
     2161 aaggatagtg agttatatcg cacacatccg aattggatta tttctacacc taatcgaaaa
     2221 cctaatcatg gtaggaacca atatgtatta aattttgggt tagatgaggt tgttgaaaat
     2281 attttcgatc aaatgtgtaa aatcattgat gaatcaaact tagattacat taagtgggat
     2341 atgaatcgtc cattgactga tgtatttgac agtcacttgt cagcagatca acaaggagaa
     2401 gtattccaca gatatgtact aggcgtatat cgcttatatg aaaaacttat aacaaaatat
     2461 cctaacatac tatttgagtc ttgttcatca ggtggtgggc gctttgatcc tggaatgctt
     2521 cactatgcac ctcaagcttg ggctagtgat gattcagatg caattgaaag gttaaaaatt
     2581 caatatggaa ctagtatgct gtatcctctt tcgtctattg gtgctcatgt atcaattgtt
     2641 ccaaatcatc aaacaaaccg tataacacct ataaaaacta gaggaaatgt agcttttttt
     2701 ggtgcatttg gatatgagct tgacttaaat gaattaagtg aaggcgattt gaggattgta
     2761 aaagaacaaa ttgcctttta taaaaaatat agaaccataa ttcataatgg tactttttat
     2821 agactattat caccattcga tgatgataat caaaccgcgt ggatggttgt tagtcatgat
     2881 aagaaaacgg ctattgttgc tcattataaa acattaaatg aggtaaatgc tccttaccgc
     2941 cgactgaaat taaaagggtt agataatgac ggactttata atgtaggaca aaatagtttg
     3001 cgatctggaa tggaattgat gagagcagga ctagtctgtt cagatgcatc tagtggacaa
     3061 ataaatgatc agaatcgacg cccagaaaca tttgattttg attccaagat ttgggttata
     3121 acacaggagg aaaagtagag atgaataacc aaaaaagctt acttcaaaaa ggagcaatct
     3181 taagtatttc actaatgtta acaagctctc aagcaattaa tggtgttatt ccacaaatga
     3241 aacagtcttt ggggatatca caaagtcaaa gtgaactttt aggaactgca ccaagtataa
     3301 ctgttatttt gtttatctta ttatctagtt actttgccaa aagaatagga atgaaaaaga
     3361 caataatttt aggtttatta ttagctggta taggaggaat tattccagta tttacagcta
     3421 ccttccaaac cgttcttatt agtcgagtaa tattaggtgc cggtttagga gtatataatt
     3481 cattggctgt gacatatatc agctctttat actctggcaa tactagagca acgctattag
     3541 gtattagaaa ttcaatggaa gctttaggtc agacaatcct tatttttctt gctggagttt
     3601 tagtgaacat taattggacg gcttcgtttt tagtctatgc aattgcgttt cctgtagctc
     3661 tcttattcgc gctaaaagtt cctgaaatta gagatgaaaa tagtgacata cctgaaaatc
     3721 atgtaaaaga aaagatgaat ccaatggttt ttgccctagt tctatttgca atcttactcg
     3781 taatgaatag tattgctatt tctgtacgat ttgcatcaat tgcaactgaa attaaagggg
     3841 taaactttaa tgctagtaac tatttagctc taatgcctat cttaggaata ctagcaggat
     3901 ttatgtttgg accaataaat aagtggatag gaaaaggaac cctatacttg ggaattatat
     3961 tctttataat ttcaaatttg ttaattgctg tgtcaaacgg taatatgacg ttcctgctaa
     4021 cgggtttatt tttatcaagt ataacaggat catggtgctt cccatttatc tttagtaact
     4081 tagataaagt cactacaaag aatacaatta attttgctac ttcattgatt tttattggct
     4141 gtaatattgg taattttata gctcctattg ctatgcaatt agcacaattt ctaacaagaa
     4201 gtacaaattt gactgctcca ttttttgtat tcgcaggaat atttattgtg atcctatttg
     4261 taacattttt tgcgacaaat aaaaaaaata ctagagtaac ccgtggtgct agttaatttt
     4321 aatatcctaa agaatatctc aaagttcctt caaaacgtcg ttgtgctcga cttaatagaa
     4381 cactgtaagc cagtaatatg ctttcaaatc tactctctag ccctttaaca gaacgtgagt
     4441 tgaaattttg acaacctaat ttttctaagg aagaaaaaac agtctcaact gtttttcttt
     4501 gttttccaag taagctgtgg tctagttttt ctgatttatc catgttttta cgtggcggaa
//
LOCUS       DAZKLA010000169         4425 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000169 DAZKLA010000000
VERSION     DAZKLA010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.173"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            690..1658
                     /locus_tag="ACG85N_002679"
     CDS             690..1658
                     /locus_tag="ACG85N_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0225385.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1726..2484
                     /locus_tag="ACG85N_002680"
     CDS             1726..2484
                     /locus_tag="ACG85N_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFQ0225386.1"
                     /translation="MYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIKEIG
                     LDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELIH
                     MGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQ
                     FLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYF
                     LEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ACG85N_002681"
     CDS             2552..3262
                     /locus_tag="ACG85N_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0225387.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="ACG85N_002682"
     CDS             3298..>4425
                     /locus_tag="ACG85N_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0225388.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAZKLA010000170         4419 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000170 DAZKLA010000000
VERSION     DAZKLA010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.174"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(334..735)
                     /locus_tag="ACG85N_002683"
                     /pseudo
     CDS             complement(334..735)
                     /locus_tag="ACG85N_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
     gene            complement(924..2243)
                     /locus_tag="ACG85N_002684"
     CDS             complement(924..2243)
                     /locus_tag="ACG85N_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFQ0225389.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            2500..2970
                     /locus_tag="ACG85N_002685"
     CDS             2500..2970
                     /locus_tag="ACG85N_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFQ0225390.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            3192..4295
                     /locus_tag="ACG85N_002686"
     CDS             3192..4295
                     /locus_tag="ACG85N_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFQ0225391.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT         1435 a          736 c          916 g         1332 t
ORIGIN      
        1 tgattcccct tgaaaatgaa ttgaggtaaa cgagaagaat tcaataaact ggatgataaa
       61 aatataatat gactataaat tggttacagc ttcaacctat aagttgtaat aaattagcct
      121 aaaagtaatt tattaatgat gaggtgaatt tgattggtac aacaagatat atcacttaga
      181 gagggagaag cagcactaca aactactttt caataagtag gaattataga aattttactc
      241 aaaaaatctt cagtaaacaa tttggctaaa taagattcag ttgttatacg agaaggttac
      301 ggttcttctt aactaagctt agtatgtttt tattcaaggc atgattttct ctatgtgatc
      361 attcagtttc tgtaagagtt ctaataattc ttttttggag tacttgtaat tgctttctac
      421 ccaccgattg agaacggata gacaaccttg gacgtggtac gtagtaaaga atctcatttc
      481 ttctgtgata atcgttttct tgtcttcata tagccatatg ttcagatagt tttcttcaat
      541 aacttgttta actttcttct gaaacgaaat atcaccatgg ttgaggataa ataagcgata
      601 taaaaaagta tttgcgtaaa catagtcaat caagcgagtg tatagttcct catagctatg
      661 ggctggatca taagagagaa tttcatttag atcccgaaaa aagttgcttt ctaattgatc
      721 cctgaataat tcatagcttt aattctttgg cacattttat tgaaatatgt atcactaata
      781 tccctatcaa ctgattttga ctaaaaagtt acttcaaaca ttcactgttc aaaaatattg
      841 aggaataaat tttgggtgta aggcattttg agcatacttc tccctggata acttcccagt
      901 aaaaaaatcg ttagattgtt ggattagatc cattgactgg ctcgcctcag gcgagcaaag
      961 accctgtaga attcattctg atacacatga taactagata atttgagata gactcgtcta
     1021 cccgtttgga ctaatttccc agcaactttc agaaacttca atcgaataga atgaatggtc
     1081 attccctttt ggacttcttc aaagccaatc gttcttaaga agttgactaa gttgtaagct
     1141 atcaagctga gcatcattcg gacatgattc tccaaaaagc gaggactgtc tgttttgtca
     1201 aagtaaaagc cagctttcgc ttctttaatg aagttctcca ttgtgccacg tttggcatag
     1261 atagaaaaga tggtatcagg agaaacattt tctgatagat tcgtcacgat aaattcatgt
     1321 cgaaagagta gttcgcccgc ttcacgtgtt gagcgtatac acacgcgacg actttgtgac
     1381 caggattgtg cttgataagt ggtcgagaag tactgaactt ctcgttcttc ccacttttga
     1441 ttatcgccat aaagtactga tttctcagct atttgaccta gtctacgatt attcttcagt
     1501 cgaataacat aatgactctt ttttgattca cacgaatcat acacaccagg tgtagcgaac
     1561 ccgctgtctc cacgaaccaa gatgtcagtg tttggtagag agtgattata gtgctctaat
     1621 aaaggtgtga gaaactcctt tacgcctttt gatgtatatt gatttcctga acgtagttca
     1681 gcttttaaga agtcaccagt caatccgtca aaagctacca atggatggta accataggtt
     1741 tggtagtgtg tattataatt cgtttgttct tggtgaccaa atgtatctga atgggtcgaa
     1801 tctaaatcaa tgattaattc cgtatcatta cggatgaggc gtgctttatc gataagttct
     1861 tggttcaaag cttgaagttc atggatattc tcctcagata gtcgatctaa aaatcgggaa
     1921 agtgaagatt gagaagcgag ttctttccta tctaaaacag ctttaaacac aggatcttgt
     1981 ctcaggagat tagctgcaga atcagctgag taaccagcaa ttaattgcat aatgaactgc
     2041 tcaagtatgg ataagttgtc atgagtaaaa tacgctcgtt cgtcttcaat gtgaatgtgt
     2101 tgcttagcca gttcagaaaa accaaagatg ttcatcagct ctttcactag gacgagaccc
     2161 gaatcactcg ataattgacc acctgtatga gaaatagtga tatttgaatt gaattttact
     2221 tggtttttgt gtaagctagt catgaaaaga actcctttct tttggttggt ttagtcactt
     2281 taaccatagc agaaaggggt tcttttttca tcacttaacg ggtgaagatg aaaaagtaat
     2341 tgtaagctgc cgtgaggcag tttcacatgg ttttaattaa aagtatgaat tattcaggta
     2401 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
     2461 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
     2521 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
     2581 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
     2641 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
     2701 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
     2761 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
     2821 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
     2881 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
     2941 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
     3001 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
     3061 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
     3121 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
     3181 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
     3241 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
     3301 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
     3361 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     3421 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     3481 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     3541 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     3601 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     3661 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     3721 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     3781 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     3841 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     3901 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     3961 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     4021 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     4081 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     4141 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     4201 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     4261 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     4321 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     4381 tttgaaacga aaaaataaaa ttgaaatatt ttttcatac
//
LOCUS       DAZKLA010000171         4298 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000171 DAZKLA010000000
VERSION     DAZKLA010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.175"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACG85N_002687"
     CDS             complement(<1..149)
                     /locus_tag="ACG85N_002687"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0225392.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACG85N_002688"
     CDS             complement(593..1714)
                     /locus_tag="ACG85N_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFQ0225393.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACG85N_002689"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACG85N_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFQ0225394.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACG85N_002690"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACG85N_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFQ0225395.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
BASE COUNT         1418 a          774 c          812 g         1294 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatct
//
LOCUS       DAZKLA010000172         4282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000172 DAZKLA010000000
VERSION     DAZKLA010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.176"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1257)
                     /locus_tag="ACG85N_002691"
     CDS             complement(37..1257)
                     /locus_tag="ACG85N_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0225396.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ACG85N_002692"
     CDS             complement(1277..1888)
                     /locus_tag="ACG85N_002692"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0225397.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ACG85N_002693"
     CDS             complement(1931..2863)
                     /locus_tag="ACG85N_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0225398.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="ACG85N_002694"
     CDS             complement(3284..4144)
                     /locus_tag="ACG85N_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0225399.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DAZKLA010000173         4196 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000173 DAZKLA010000000
VERSION     DAZKLA010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.177"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            151..1452
                     /locus_tag="ACG85N_002695"
     CDS             151..1452
                     /locus_tag="ACG85N_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0225400.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1479..1739
                     /locus_tag="ACG85N_002696"
     CDS             1479..1739
                     /locus_tag="ACG85N_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0225401.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2738..3973)
                     /locus_tag="ACG85N_002697"
     CDS             complement(2738..3973)
                     /locus_tag="ACG85N_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0225402.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1209 a          670 c          867 g         1450 t
ORIGIN      
        1 aaaacttatt ttcaggattc ttaaaaataa atttgattat ttaagagaat atattgaaat
       61 aaaatttctt aattatataa ttagttatga acattctttg cgttgtcatg caatgaatgg
      121 ttcatatttt attgaaaagg agtggtttta gtggaaaagc tgatgaaatg ggtcgaagag
      181 aaacttgtcc caccaatggc aaaaattggt acacagcgtc atttattagc aattcgtaat
      241 ggggttgttt caacattgtc attgattttg attgggacat tttttatggt ctttatcaat
      301 ttaccgtttc ctggatggaa tgaatttatt gccccttatt cggcaacgat tgttttaccc
      361 tttaggatga cgatgggatt gatggctatt tatgcaactt ttgttatggg ttcggatcta
      421 gctaaatcat atggattaga tagtgtgaca ggtggtatat tatctttagg aacgttcttt
      481 atgttacaag ttcctgtaaa cgttttaact cctgaagagg cacctcttgg ctgggtctta
      541 cctatgagtt ctttgggagc atctgggatg tttgcgggga tcttatctat gatttttgca
      601 gtggaagtat atcgtttctt taaaaatcga aatattacta tcaaaatgcc agaacaagtg
      661 ccacctgctg ttgcccgtag ttttgaggca ttgattccag gtgcagtcat tttaacaatt
      721 acttggctga ttcgaagcgt cgcgggcttt gatgtaaacg ctgccttatt atcgttattc
      781 gagcctttaa ccaatatttt agggaataat ttgcttggtg ttttactacc aatgtttttg
      841 atccatttgt tgtggtcatt tggtatccat ggcatgagta ttgtaggggc tgttgttcgt
      901 ccaatgtggc tgatcatgtt ggatgagaat gcaaaggcat tggcagatgg tacagctgcc
      961 acccaattac cctatatcgc tcctgaacag ttttatcaat ggacggtaac aattggtggt
     1021 gcaggagcaa cgatcgtggt atctgttttg tttatattct tctgtaaatc aaaattcttg
     1081 aaagaggttg gtcgtttttc tattattcct ggtatcttca atatcaatga gcccataatt
     1141 tttggagctc ctatgatgtt gaatccttac atgttcatcc cgttcaactt agttccttta
     1201 gttttgacta ttatcagtta ttttgcagtc aaattagaaa tggtcaatag atttactgtt
     1261 ttgccaacat ggacattacc agctccaatc ggcggttatc tttcagcagg aaatgactgg
     1321 cgtgttgtta tcttgatcat gatcaacaca ttgatagcat ttattatcta ttatccattt
     1381 gtaaaagctt atgataaaaa gatgctgtca gatgaacaaa gtcgtggaga aaacgaagtc
     1441 cagactgtct aagtttggtt tttagaaggt gggaaacaat gtattggctg attttatttt
     1501 ttgtttttat ctttttatta acggctagtc accttatatt gaatatgcta gctacatatc
     1561 atattcaaat caatcgatgg atttgggcac tggcatcatt tttgatcgtg attcttccaa
     1621 agatcattgt accgcatatg aatgttttgt tttcatgggg gacatatgta ctttgtggaa
     1681 tttttgctat caattttatg atcgagcagc atagatggtt tgtcacatcg aagctttaaa
     1741 tgttctaaaa gtggttgtaa cagaagtggt tagcttcaag aaataagaag aaatttacga
     1801 aaattgcttc ttaaattttt gtgaaatgtt ggcttatttt tcgtttggtt gaacttatta
     1861 ccgcagatgc aagcttttga ccaccgttta ttcggaatat gggactgtga caaggctttt
     1921 gtcacagccc ttttttacat aaagattatt attgttttaa ctgttttggc agaaagaagg
     1981 aggaaaaaat ggaaatttta tcagcaatgg atttagaatt agtagatcta ctaccaaagt
     2041 gatcaagtca aatatagatt tgggagctta caaccatata gtaggggctg cagtatggtg
     2101 tatgaatggt aatatgtatg atgtgagtgt ttattcaaat catgaaacgt gtatagaaat
     2161 aattgtgctg ggaatagcaa tcagtcatgg tgaacgtgac ttttagtgta tcgttgctga
     2221 cgacatagaa cattctgaca tgatctactc aatatgtggg gattgctggc agtttatata
     2281 ggagtatgca tcggattgcg agatcattgt ctcaacagaa gagggaggtt gattatcaaa
     2341 gataaagtgg taaatcctac atttgattta gtaattgttg aggggactag cggcacaaat
     2401 ctctacaaag cgatgcttct agatctatat gacacatttt aagaaaatgt atttgcctaa
     2461 ttattatggt ctttcttttg aagaaacgtt gagatgatcc aaccagagaa aggtgtcaaa
     2521 gaatttgact aagaataaat ggcagacgct acctatgctc atttggatag cgtcagatct
     2581 tttattttaa ggatgatttt gtgtttttta gacaccgtaa tttaagtaac tctaactttt
     2641 caatccacta tttacttttt ttgtattttc actgtctggg tctcgaatag cgaaattact
     2701 gatgagtctc tattaaaaaa gctttttcta cttgaaacta tattgggtgg ttgctcagga
     2761 aggtttcgat ttcttgaatt gcttgttgtg gaccatttat tggaaaaacg gcttgcagcg
     2821 tgcgtgcctg ctgacgaatc ttgggtgaag aaacttcgcg cagagcaatc tgcagttttc
     2881 tttcagtcac tggttttgtg agggcgattg cgtttccgta ttttacacaa tagtcaatat
     2941 tacagctttg ctcaaattgc atccccacac cgataaacgg tttgccggat tcacaggcag
     3001 tttggacggt cccttccccg ccgtggataa aagaaaaatc aatgaattca tgcaaaagat
     3061 gactagacag atattctgtt attaaaagat cttctgagtc agattgaatg tcatcaagca
     3121 gatagtcaat aggtgcaata accgctagat ccggttgttt ttgtaagaag aacaaaattt
     3181 tgcgtaacat acgcggattg cctgaactac ccattgcgaa atagataata cgttttcttt
     3241 cttgttgctt tcgctgtata aaagttagca cttcttcagg caaggaagta ctgagcttag
     3301 caaaaattgg accaacgatt cgataatttt ccggcagctg ttcttcttcc aaaaatagtt
     3361 taggcgttgt gatgagattt agatcagcat ccatcatttg taaacttgtt tttggtaaag
     3421 gaagcatttc tttttgtgca gcgtgtttga aggcattggg gatccatcgg gtatttacta
     3481 tacgcttttt tgtccagtgc caaaggggat tgcttaaagg gccgaagaaa cgtaactcgt
     3541 ggggcatcag cgcatgctct gattgaaaaa aaggttcact caaagcatag ggtttaacat
     3601 atactaaagg aatcttttta ttgcgggccg ataaaaaaat tgtcacattc gaacctgtca
     3661 caatcagtcg aggctcaaac ttagcaatca gactctgttc actagtgata cgagaggcaa
     3721 ccatttcttt ggtaaaaggg tttttaagtg tacgcaactg gtcaaatttt atgatttgtt
     3781 cttcttgttt tttcgtaagg atcggcgcta gtaaatgata ctcaaagcca ctttctgtga
     3841 tcaaaggagc aaaagtttta gaaaagccaa agaatacaca gtaataacgg tctttcatag
     3901 ctttggcaac ctcaatcatt cgtgttgtct cagctaaatt gaatactgca ggagcaaaaa
     3961 gaattgtctt catgaaatcc tccttattgg aatgagtcta tcccttcgtt tcttactagt
     4021 atactaaaaa acacagcgtt cgagtagcct atgtatattt tgaatattaa actgtctctc
     4081 gctgttttca caacttctaa atttaaattt tgtaatcaag ttgaaactcc aattttcaaa
     4141 aaaacacgca acaatttttt gatcgattta tatgttgtgc cagataaata cgagaa
//
LOCUS       DAZKLA010000174         4161 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000174 DAZKLA010000000
VERSION     DAZKLA010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.178"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..125
                     /locus_tag="ACG85N_002698"
     CDS             15..125
                     /locus_tag="ACG85N_002698"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225403.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
     gene            130..2259
                     /locus_tag="ACG85N_002699"
     CDS             130..2259
                     /locus_tag="ACG85N_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0225404.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            2256..3263
                     /locus_tag="ACG85N_002700"
     CDS             2256..3263
                     /locus_tag="ACG85N_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0225405.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            3280..>4161
                     /locus_tag="ACG85N_002701"
     CDS             3280..>4161
                     /locus_tag="ACG85N_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0225406.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKN"
BASE COUNT         1372 a          671 c          946 g         1172 t
ORIGIN      
        1 aaaacggtca atgcttgatg caagacttgt atgggcgagt aggtgtggtt caagttcatc
       61 cagtctttga agaactcttc catgcttttg acactcgtcc gccggttcaa gagagaatgg
      121 agtgagggga tgaaaaagaa actttttcgg attgtgggaa tcgtttttat tagcatcgtc
      181 ctattgacag ttctattgag tttagtcggt acggtagctg aggcgacagg tctggttgat
      241 gatacagttg aagccggaaa cctttattcc aaatattctc tgaacaatta tcaactggat
      301 ttctttgtgg atagttcttg ggattggtta ccttggaatt ggggggatgg cttagggaaa
      361 agcgtgatgt atggtcttta tgccattacc aattttattt ggacagtaag tctgtattta
      421 tcaaacgcca cgggctatgt ggttcaagaa gcttacaagt tggatttcat ttcggatacg
      481 gctaaaagta ttggaaagaa tatccaaact ttagccggta tcactgaaaa tggattacag
      541 acttcgggat tctactttgg gttcctatta ttgatgattt tagctctagg agtttatgtg
      601 gcgtataccg gattattaaa acgagaaact accaaagcag tccgaaccgt cattaatttt
      661 gtgatgattt ttctactctc gggttctttc attgcgtatg cgcccaccta tattacaaaa
      721 atcaatgact ttagctcaga cgttagtgaa gcggctctta gccttggaac caagattgtt
      781 gtacctaatt cagaaagcca gggaaaagat agtgtggatt tgattcgaga cagtttattt
      841 tcgattcaag ttcaacaacc ctggttacta ttacaattcg atgattccaa tatagaagaa
      901 attggcgagg gtcgggttaa taagatttta tccgtgagtc cggatgaaaa taagggtaag
      961 gatcgggaag aagcagtcaa agcagagatt gaagataatg acaatgcgaa tctcagtatt
     1021 accaagacca tgaaccgttt aggaaccgtg gtctttttag tgttgtttaa tattgggatt
     1081 tccttttttg tgtttcttct gacagggatt atgttgttct cacaaatcct tttcattatc
     1141 tttgcgatgt ttctgcccat tagtttctta ttgtcgatgt tgccgacgta tgagagtttg
     1201 ggcaaaaggg cgattattcg tttgttcaat acgattatga tgcgagcggg agttacctta
     1261 gtcattacga ctgcattcag tatctccacg atgtttttca atatttcggc cacctatccc
     1321 ttctttatgg tagcttttct acaaattgtg acctttgccg gtatctattt caaattaggt
     1381 gacattatga gcatgtttaa ccttcaaagt aatgatagtc agtcaatggg aaggcgagtc
     1441 atgcggaaac cgcaaatgtt gatgaatcga aaattgcgac agcttaatcg aaatgtgggt
     1501 cgaacttttg cttttggtgg cggtgcagca gttggtggga aactagctac cagttctaca
     1561 aaagggaaag gtagttcaaa aatcaataga gtgtccaata gaaagaacca ttccgcgaga
     1621 agcattaacg agctaaattc tgatctgaaa aaacaagaac aaccagtggg cacgaagaaa
     1681 gaatcacgaa taaatttagt tggtagaaaa gttggaaaag tactcgatac aaaagaaatc
     1741 gtgaaggaca aagcgaaaca agcaaaaaat caagtaaccg atgcaccaac taacttgaaa
     1801 tacagccttc ataaaggcat ggaaaagacg aaaaaagtac cagaggactt taagcgaggg
     1861 cctttcgaag aaaaagcaaa tcgaggggag ttgcgagaaa aacaacgaca acgaagagac
     1921 gcaaaaatgg atgaaaaacg caaagtattg aacgaagcat ggaatcgtca tgagaaagga
     1981 agggaaaatg ttccgataaa aaaggagata aaaccacaga aaaatgaact accagaaaat
     2041 aggtctttaa aaccaagggt acaagtacat tcgaatcctg aaatcaaacg gaaactttct
     2101 agtcaagaag tcattccaaa cggaagcact cagccactaa gcaagaacaa ttcctttcaa
     2161 caagtgaaac agagattcac ttttccaaag cgaaccaatc aacaagtaca aagaaaaaag
     2221 aacactcgcc caaaaccaaa gccaggtgag aaaagatgaa cttaaaaaaa ttgagtatta
     2281 tttcgttaac cttcctgacc atctcttttg tgggcatctt gatgtgtgtg ggtttgttgt
     2341 ttggtgagga cggtgaggga agtggtagtt ctggaacttc tcctggaggt agtagtgtct
     2401 cagaagtagt tttgaaacac cgttcaatgg tggaaaagta tgccaaagaa tcagatataa
     2461 cagagtatgt ttcgattctt ttggcgatta ttcaagtcga atctggaggt acaatggaag
     2521 atgtcatgca aagttctgaa tcagcaggtt tatcaccgaa ttcattaagt accgaggcat
     2581 ccatcaaaca agtgtgtctc tatttttcgt ctttggttaa gtgttccaaa gaactaggat
     2641 gcgatcaaga tagtatcatt caggcttata actatggtga cgggtatttg gattatgtcg
     2701 cgaaaaacgg aaagaagcat agttttgctt tagccgaatc tttttcaaaa gaaaaatcgg
     2761 gtggtcaaaa agtcgattat cccaatccca tcgcgattaa ggaaaatagt gggtggcggt
     2821 ataactatgg caatatgttt tattgcttat tagtgaagca gtacctaact acaacacagt
     2881 ttgatgataa aacagtccaa ggaatttttg acgaggcgtt caagtatgaa ggaacggctt
     2941 atgtatttgg tgggtcaagt cccggaactg ggtttgattg tagtggttta actcaatggt
     3001 gttatgccaa agttggtttg aaactccctc gggtggctca ggatcaatat gatgctatga
     3061 ctcatattga cttaaaagat gccaaaccag gcgatttgat tttctttcat tcaacttatg
     3121 atgccggtac ttatgtgact catgtgggca tttatgcagg agagaacaga atgtttcatg
     3181 caggagatcc ggtgggttgg accaatctta ccgatagcta ttggcaacag cacattattg
     3241 gtgctggtcg gtataaacaa tagaaaagag gaatggaaaa tgaagataaa aattgagcga
     3301 agtaacaagg aaaagattcc aaaaaagaag aaagaacgag tagtatccgt gggcaagcat
     3361 cgaaaaatgg tgcttgccct ttggctttta ttgtcttgta gccttgcttt tggaatctac
     3421 aaaaacttca cggccattga tcagcataca actcatgaga aagtggtcgt gaaagagaaa
     3481 atagtgaata cctctggagt agaaaatttt actgtagatt ttgtgaaaga atatttttca
     3541 tggaagaata acaaggaggt aattgaaaag agaatgacta atcttgagca gtaccttaca
     3601 gaagaagggc tcgctttgag ccaagatatg gttcgagcgg atattcccac tagttccgag
     3661 gttgtatcag taaaaattct agatgttgaa aaacactcgg aagaatttgt cgtttcattt
     3721 gtggtagatc aaaagattac agaagggaaa aagacacaat caatttcttc tgcatatcgt
     3781 ctgatgatct ttgaagatga aaaaggcaat catattgtaa cgagtttacc cactatgatt
     3841 ggtaagccag aaaaggcaaa acataaagta agacaagtaa aaagtgattc aaaaattgat
     3901 tcaaagacaa cggaagaaat caccgagttt ttagagacgt ttttcaggct ttatccagca
     3961 gcctcagaaa aagaactaga gcattatgtt gaaaaagacg cgatgcaacc aattaatact
     4021 aatttgaaat ttgtggaatt agtgaatcct atttacaaac aaaagggtaa agcgattcaa
     4081 gtgattgttg gtgttgagta tcttgatgag ttgtccaaaa caacaagtgt ttttcagtat
     4141 agattaaaac tatacaaaaa t
//
LOCUS       DAZKLA010000175         4147 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000175 DAZKLA010000000
VERSION     DAZKLA010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.179"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..388
                     /locus_tag="ACG85N_002702"
     CDS             50..388
                     /locus_tag="ACG85N_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225407.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYEWLADYLNLPDEDNYS
                     EMLERMMDVLNSVINADLAKSSVLTNEFVHRHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            392..754
                     /locus_tag="ACG85N_002703"
     CDS             392..754
                     /locus_tag="ACG85N_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225408.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENATMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            975..2381
                     /locus_tag="ACG85N_002704"
     CDS             975..2381
                     /locus_tag="ACG85N_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HFQ0225409.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQTNIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPNLAVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDVHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NEREKALVASERVKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
     gene            2828..3802
                     /locus_tag="ACG85N_002705"
     CDS             2828..3802
                     /locus_tag="ACG85N_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0225410.1"
                     /translation="MSKRGTIVNEWEKLNRISNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYVEILKF
                     HEGKGRIDFNPNKINQFLSGSMKNFIHDLFLEPHFSRADIACDIVDIPDDFVSQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWRLELQ
                     LRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKNTKKK
                     YRDLLKQESQNDELTNHLRESFAESADDLKRELDTWLQGLDVTEVGAE"
     gene            3799..>4147
                     /locus_tag="ACG85N_002706"
     CDS             3799..>4147
                     /locus_tag="ACG85N_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225411.1"
                     /translation="MSNPVEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQFTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQ"
BASE COUNT         1446 a          599 c          928 g         1174 t
ORIGIN      
        1 aaaaaaacga tcagttgtta ctagaaaatc aagaattaaa aaatcgaaaa tggtggcaat
       61 tctggaagtg aggtgtgaca tggataaaat tgatttgaga gaagaaacag tcgatagaat
      121 tgaattgatg tatgaatggt tagctgatta tttgaatttg ccggatgaag ataattatag
      181 tgaaatgctt gaacgaatga tggatgttct caactcggtt atcaatgctg atctagcaaa
      241 atcttcagtt ttgactaatg aatttgttca taggcattta gcgcaaacaa ctgatgaatc
      301 agaaaaagaa ttttggttga aattaatgag tcatgttatg cagttaaacc aaatttccgt
      361 tattaattcc aaagaaaagg agatgtagtt tatgggaatg actttagcag atattaaagc
      421 gcaagaagaa aaattaaaaa aagaacaagc gaaacttcgt caacagaaaa aacgcttgat
      481 tgagaaagaa aatgctacaa tgggcaaggc tcttcgcaag cgttttgggt ttgaatcttt
      541 tgctgatttt gagcggtggt tatcgtcaat ttctgacgat cagcaacacg aaaaatcagt
      601 ggataatgag tggcttgctg atgaaaaatc agcgccaagc gaacgagatt atcccgatga
      661 atctgacggc ttattcggag tgataccgaa taagaaaagt cagatgagtg agagtaaatt
      721 tacgtcaata tttaatgacg tgaatttacg ataagcgatt attttgcgtg aagcaaaata
      781 aagcgtaaaa ggagttgata gcgtgatacg ctttcggctc cttttttcgt tgtcacttgt
      841 gacagcgaaa accctcggag agcgtttgga attgtgacat ctatgtcaca attaggaaaa
      901 cgttactctt tgacaaagag taacagaaag gtttacaatc tttttattag cgacagccga
      961 aggagggttt taacttgggg ttcagtattt cgttcaaaaa gggaacaaag aatacaagca
     1021 tttctcataa taatcgtgag ttaactgata gccaaaaaat caatgactgg cataagcata
     1081 ttgatttcac aaaatcggat gaaaatatct atttagaaca aacaaatatt cgtgaaaaat
     1141 atgaagaatt attcggtgaa gccgtagaaa aatataatgc aaagcagaaa agagccgata
     1201 gaaaaatcga ggactattta gcaaaggttc gcaaggataa aaaattggaa ccacagcgag
     1261 aatttatcgt gcaaattggt actcttgatg attttcgtac tactcgtgat gatggttctt
     1321 ctactggcat atcggaacaa caagcagaac aaaatcgagt gattgccaac aaaattttag
     1381 ttgagtattt caaagaattt aaagaacgga atcctaattt agccgtctac aatgctgtta
     1441 ttcataatga cgaagtaagt ccgcacctgc atttgaatat tgttcctgtt gctgaagggt
     1501 ataaacgggg tgttcaaaag caaccaagtt ttaacaaagc cttgcaacag cagggtttac
     1561 gagcagataa agagaatggt cgagtgattt ggaataattt taggaatcag gaagttgagt
     1621 caattgaacg tttgatggca gattttggtt gggagcgtga gttagtcggt actaacaaaa
     1681 tcaaagatgt tcacgaatat aaagaaatca tgagcgagat ttcagaattg cgtcaaacgg
     1741 ccgtaaaaga gcgtgagagc gtttctgatg agttgaggta tatttctagg caaagagaag
     1801 aaaatgagcg agaaaaggct ctggtggctt ctgagagagt gaagattgaa gaagagaaga
     1861 aaaatatgct gaatgtaaaa aaagagatgg cggtcatttt ccaaaaagat attgttcctt
     1921 tagaaagttt caagaggctt cctgatggtt cgtatcattt atctgggaat gattttgttg
     1981 atttgtatca acgagctacg agagcaacaa atgccaagag aaatgctgat aacatgagtg
     2041 agcaatattt acagatgctt gagcaaaata cagaattaaa tgagtatgta gctgcattgg
     2101 aagaagagcg tcatttgtct ggtagaaaac aattagcaga tttacaagtg gaaaataaaa
     2161 agctgaaaga agaaaatcgc tctttacgcc agcgtttaga gcgattaaaa aacgcatttc
     2221 aaaaatcgat cactcgtttg tctatccgtt ttggtcttgc agaaaaggat atggggcttt
     2281 ctgaggaatt agaagtatta gagcaggaga aaaccttatt ttatcgtcaa aacgtctcac
     2341 agcataaatt agagcgttct gaagatgaga tcgagtggta gttacgatgt gcagtcaacg
     2401 gtaccggagg cggtttgtgc cgaggataag gagttgacgg ctaaaccact agcctattaa
     2461 gctgacattt gctaaaaaat ggccagtcca ttttttggcc aacctagcgt tagcgaagtt
     2521 tgggggcgcc ctttttaaag ggtgcggttt tatgttttta tatatttgca tagcaaaaga
     2581 gaagtgctat tattgagtta gcaaacaaat gaactggaaa aagagaaaaa aataagtcga
     2641 gcaacgctac caacgttctc gacctttacg gaaactactt gtactagctc cgtaactcat
     2701 actcattata cgaatatttt tagatttctt caagactaga gattttttgg taatgaattt
     2761 ttaagagtta cgtgaacttt tgaaaattct ttccagaagt ctctttttct ttgaggagga
     2821 acaaaatttg agtaaacgtg gcacgatcgt gaatgagtgg gaaaaactta accgcatttc
     2881 taacaggcgg ttaaagtatc aaagtttgac ccttccctta aaaatgcagt ggtcaattga
     2941 ccgaattacc atagtcggaa aactgaaaga aaatatttat tatcacactc caaatgatgt
     3001 gttgatttta aattttgaac agcttatgcg tttgaacgaa ggcaatggtt atttaaaagc
     3061 tgttgggaat aatgggtggc aattattaga ccaacacgag gaaaatatag cttatgttga
     3121 gattcttaaa tttcatgaag gaaaagggcg gattgacttt aatcctaata aaatcaatca
     3181 atttttatca ggttccatga aaaatttcat tcacgattta tttctagaac ctcatttttc
     3241 acgtgcggat attgcttgcg atattgtaga tattcccgat gactttgtta gtcagtatcg
     3301 tgtagttgat cctgtgtcct ttaaaccaat ttatgggcgt tctggtaagt tagaaacggc
     3361 atattggggt agtcgtgcca gtgaacgaca aattcgaatg tacaataaaa aattggaaca
     3421 agaaagaaaa cgtaaaattg ttccgccgga aataacgaca tggtggcgac tagaattgca
     3481 gttacgaagg ggaaaagcaa ctgattggca tgagatggtt catgaaagtt tggatagctt
     3541 tgctagtcca cattattttc cgccaaatac taaagttact gaacggatta tggtgaaagg
     3601 tttgctttat gatcattctg agtggggaaa tctagctaag aatacaaaga aaaaatacag
     3661 ggatttgttg aagcaagaaa gtcaaaatga tgaattgacg aatcatttgc gagagtcatt
     3721 tgcggaatcg gcagacgatt tgaaacgaga attagataca tggctccaag gacttgatgt
     3781 aacggaagta ggtgctgaat gagtaatcca gtagaaaaaa tgaccataaa aaatcttgct
     3841 gacgaattgg gtgtatcgaa aaatacagtg cgcaatcaac tggtcaattc agggtttgac
     3901 cttgctcagt ataaagaaaa tggcattatc atgctagata aagagttgat tggtgttgtt
     3961 cggtcaattt atcggtcaaa aagtgatcaa tttactggtc aattaccggt caataacaca
     4021 ggcagtgatt ttcctttaga aaaagagaat gagtttttgc gtgatcaatt agctgagaag
     4081 gacaaacata tttctgaatt aacagaaatg attcgtgatc aacaaaaatt gaccctgctt
     4141 cagcaac
//
LOCUS       DAZKLA010000176         4044 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000176 DAZKLA010000000
VERSION     DAZKLA010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.180"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(153..1658)
                     /locus_tag="ACG85N_002707"
     CDS             complement(153..1658)
                     /locus_tag="ACG85N_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0225412.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1655..2341)
                     /locus_tag="ACG85N_002708"
     CDS             complement(1655..2341)
                     /locus_tag="ACG85N_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0225413.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2534..3955)
                     /gene="gndA"
                     /locus_tag="ACG85N_002709"
     CDS             complement(2534..3955)
                     /gene="gndA"
                     /locus_tag="ACG85N_002709"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFQ0225414.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1116 a          856 c          691 g         1381 t
ORIGIN      
        1 catctaaaag atacttacat ttaattaacg gatgttattc tatttaattg ttcgctagtt
       61 tttttataat gacatccctg aatttctcac ttgcttgtat tttgtacctt attctttgtt
      121 ggatatataa aaaaacaagt aaagcacatg ctttacttgt cttctgcctt tgcgatagga
      181 ataaagattc taaaaattgt cccttgccct actacacttt ctgctaagat acgtccttta
      241 tagttttcaa ccagttgttt agcgatagac aaccccagac catttccacc cttcgttcta
      301 gctcttgcat tatccactcg ataaaaacga ttgaagatct tattaaggtc ttctttgggt
      361 attccttcgc caaagtcctg aatcgcgatc tcaaattcat tcagagtgga agaaatagag
      421 atatggactt cttttcgtgt agttgaatac tttactgcgt tatccaatat gataatgatt
      481 aattgttcaa agtgattacg ataaatttgc agggtcactt cttttgtcaa atcatcatct
      541 aaagtgatca catagtctgg atataatatc ttgaaattat tataaacttg ataggtgact
      601 tcttttcctt tagttgtttc attcgcataa tgaacatcta cttgttctgc tcttgataag
      661 tctagcatct cttgaactaa gcttttcata cggctgattt cttgcatgct ggcgcttaat
      721 gattcttcta atatctcagg atcatctttc ccccaacgat ttagcagact aagatgccct
      781 tcaataattg caacaggtgt ccttaactca tgagatacgt cttccacgaa ttgctcttgt
      841 tgttcaatat acaaacgcat acggtcaagc atctcattga atatttcagc taaatctgcc
      901 aattcatcat ttgtgtcgat ttcaggcatg tggatatctg attgcggatc tttacgtata
      961 gtatccattg tatctctgag taccttgagt ggttttaaga aataagaaga taaaaagaaa
     1021 cctaaaatac tacttaagat caaagaaaca acttctaaaa caataagagt caataatagc
     1081 ttatttcgaa tatcatagaa agaagataat tcatagaagc tttgcgcata gccgatcttt
     1141 tctcttgtac ctttagaata tataggctgg atagataaaa agccagtttt tccttctagt
     1201 gtaacgatcg ttggaacttt acgtgtagtc tgaatcaggt ttcgactgtt ttcatgggtt
     1261 ttaaaaatca agttctcgtc caaatcataa acataaagat ccattgatgg ctgccctagc
     1321 tcagctataa aggaatcgat cttcattaag tttccttcga ccagtgaagt atgatctacg
     1381 acctcatctc cgtcatttgt cgctttcgtc agattacgat acgtatttac taatgtcagt
     1441 tcttcatccg agttagccaa acgagaagct acttctgaaa tcgtacgctc tacattgttt
     1501 ctttcttttg ccacaatcag attgatagat gatttatagg taatgacagc gaaaattgta
     1561 aacacaacga atatgaagaa agaactcgca aaggcccact tgattgtcaa ggagggccct
     1621 ttcagttctc ttttttccga ttttttcggc tttatcacga gcgcatcaca taacctgttc
     1681 cgcgtacagt ttggatatag ctttcctccc cgggaacgtc aattttgttg cgaagataac
     1741 gaatatatac gtccactacg tttgtttcaa cttctgtttc gtatccccac actttattta
     1801 gtaaaacgtc acgtgctaat accacgttca cgttttccat taaagtaagc agcaactcgt
     1861 attctctttt tgttagttca attacttcat ttccgcgacg tacgacacgg ttttcttttt
     1921 cgatagtcaa atcgcgataa gtaatagtag tttgtttagc cacattttta tcgccttcaa
     1981 tatcgattcg acgcaacaat gcacgaagac gtgctaataa ttcttcgata gcaaatggtt
     2041 tgacgatgta gtcatctgct ccgtggtcaa gtccagatac acgatcgatc acagaatctc
     2101 ttgcagtcat cataatgatt ggtgtatttt tgacttggcg tacacgtctg cagacttcta
     2161 aaccgttcaa ttctgggagc atcagatcca acaatatggc atcccactca ttatttaatg
     2221 ccgcatctaa accagttcgg ccgttgtagt ggacttctgc gttataccct tcatgcttca
     2281 actctagctc gacaaatcgt gccaagttct tttcatcttc aatgattaga atattgctca
     2341 tttattattt tatcctttct attctactgg ctttttaaaa actctatcat tataccgata
     2401 atatgcttaa aaatctatag tcattttatc atggatagca attttaaaaa agagctgaag
     2461 tctactcata acaatcgact tcagctcttt cgtcctcttt ttattttctc atttcctaac
     2521 agccgaaaga ctattattct tcatggtacc atgagtaatg gaagattcct tctttgtcga
     2581 cacgctcata ggtatgtgcg ccaaagtaat cgcgttgggc ttggattata tttgcaggca
     2641 gacgttctgc tctgtatgaa tcgaagtaag cgatggctga tgagaaagtc ggtacaggta
     2701 cgccagcttg gacagccaag gcaacgacgt ctctgactga ttgctgatat tttttcgtga
     2761 tttcaacgaa gtaatcatct agcaataaat tttcgatctc aggatttttg tcataggcat
     2821 ccgtaatttt ttgcaagaat cgagctcgga tgatacatcc agcgcgccag atttttgcga
     2881 tttcaccaaa tggcaagtcc caatcaaatt cttttgaagc gacacgtaat tgagcaaatc
     2941 cttgggcata actcatgatt ttactgaaat acaacgcttc tcggattttt tcgaccaatt
     3001 cttttttgtc tcccgtgaac tcaaaatcgt tcgtacgaga caaaatcttg cttgcttgta
     3061 cgcgttcttc tttataagca gaaatatagc gggcaaatac agattcagtg atcaatggaa
     3121 gtggcacacc taagtcaagc gcgctttggc ttgtccattt acctgttcct ttattcccag
     3181 ctgcatcaag gatcacatct actactggtt tacctgttcc ttcatcgtct ttacgtgtca
     3241 gaatgtcagc agtgatttct atcaaatagc tgtctaattc gccttgattc cattctttaa
     3301 agatatcagc catttcttct actgaaaggt ttaaaatatt cttcattaag tcataagatt
     3361 cagcaatcag ttgcatatct ccatactcaa taccgttgtg taccattttc acataatgtc
     3421 cggctccatt tgggccaata tatgtgacac atggtgctcc gtcttctgct tttgcagaaa
     3481 tttgctctaa gattggtgca actagcgcat aggcttcttt ttgtccacca ggcatgattg
     3541 aaggtccttt taaagctcct tcttcaccac cggaaacgcc tgtaccgata aagttgatgc
     3601 cagagttggc taactcttca ttacgacgca tcgtatcttt aaagaatgta tttcctccgt
     3661 caatcaaaat atcgccttta tcaaggtgag gaagtaactc ttggattgtt gcatctgttg
     3721 ccggtcctgc tttgaccatt agcaagatcc gacgtggttt ctcgatagat tctacaaatt
     3781 cttcgattgt atatgttgct ttgaaatttt tatctgggtg ttcttctact acagcggttg
     3841 ttttagcccc tgtacgattg aataaagcca ctgaatagcc gcggctttct atgttcaagg
     3901 ctagattttt acccataacg gccataccga caacgccgat ttgttgtttt gacatatgaa
     3961 ctcctcctac atacgggatt tctccctaat ctcttacatt atagcttaac acggctacat
     4021 tgcaaactaa agaacaacat attt
//
LOCUS       DAZKLA010000177         4001 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000177 DAZKLA010000000
VERSION     DAZKLA010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.181"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(214..363)
                     /locus_tag="ACG85N_002710"
     CDS             complement(214..363)
                     /locus_tag="ACG85N_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225415.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            825..1037
                     /locus_tag="ACG85N_002711"
     CDS             825..1037
                     /locus_tag="ACG85N_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225416.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1315..3114)
                     /locus_tag="ACG85N_002712"
     CDS             complement(1315..3114)
                     /locus_tag="ACG85N_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFQ0225417.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3238..3834)
                     /locus_tag="ACG85N_002713"
     CDS             complement(3238..3834)
                     /locus_tag="ACG85N_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0225418.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1435 a          709 c          686 g         1171 t
ORIGIN      
        1 aatattatca tcgaaacagc taacaaaatt gtcataaaaa acgagatggt tgatattaga
       61 acttgtgttt ttgtttaggt ctagagataa agagagacaa tttccaaata tatgtaatga
      121 atacaaaaag acgtttgtcg cggattaccg ttacaaacgt cttttattga ttttccgaga
      181 tctcatcagt cgccatttgg aatggttcac tccttatcta gattcgttta atgctcggac
      241 tgcttgttcg attgtaatac caaacgcaac gttattttcg ttttgtgcat caatcgccat
      301 aaaacggtta gaagttgtat caaaagctac gcggtattgc atatttgttt ttgtaaacgt
      361 cataaaattc atctcctttg taatgtaatt tagttcttga aagtttcctt taaactttcg
      421 aacttatacc aagtttagca tcgtacgttt tttgatgcaa ttaaaaagca tttatcattt
      481 tccttattta ttttttctta aatggtttag ctattttctt ttaatccgat aagtagccca
      541 atcatcaagg ttacaaatac tattgcttga atataaatca tatgctcacc tctttataaa
      601 taacttagca atataaagaa aaaaacgcac gaacaacata ggaatataaa caaacgtttt
      661 tgtcacaacg tctaagatcg tgttaaaaaa tagttatcta aaaagcagaa agatactcaa
      721 aataaaaaat aaaaaaatgg ataaaaacgt tttcaaatac aaggtaattt gttattataa
      781 aataaagaaa aaacaaaata aaaataaaga aaagagggaa tgaaatggta aaagcagaag
      841 taggaagatc ttatatatgc aaaccgatcg gagtatctca tgaactaatc ggctgtattg
      901 aacgtacata tatcaacacg gctgtcgtgt atgttgaaag ttatgaccaa cgagactatc
      961 aacttatcaa tgaaagcaag tatcgcatgc tcgtaaaatt ctgtgatatt agcgcacctg
     1021 ctgaattggt ttcttaaaca gtatttttga gaattcgcaa gaactccctt ataatataaa
     1081 taaagacaga agggagcgag aagatatgcc aaaaagaaaa agttcaggga aaatgggaat
     1141 tattcttgca gtgatctctg caattcctgc tattttagct attgttcgtg cgatgaagga
     1201 atcaagagaa agttaagcac acattatgag cgtcaaacaa aaaacgcata tacctgcggt
     1261 ggcaggttat gcgttttttg ttgtataaag acttttttca ttaacgattt ttgattagcc
     1321 gactcccaaa acaaaattta gcagtttatc tgaataagtg atttcttcat ctgtttgttt
     1381 cacactttca tttagtagtc ccatctcatc ggttatgata ccatccacac cataaaacat
     1441 catgcgactc atagcatctg aatcgttgat tgtccataca tatacttctt ttccgtcttg
     1501 gtgggcagaa ttaatgaagt tacggttgat cgtactatat tccattgtca gaaaatcagc
     1561 aggcgtcaca gggggcccaa caatattgaa aggtaagatg tagcccacat aaaagtttgg
     1621 ttctgcttct tttaaatctg aaacggtttg gtacgtcaaa ctttgcaaaa tatgatggtt
     1681 ctctaagata actttgcggt atttatggac aaatcgttcg acaaggtcat ccgaatcttt
     1741 tttagaggtc ttgatctcaa taagtaattt ttggtttagc ttatgtgctt tttccaaata
     1801 agaatcaaaa gaacaaacag gggcttgcac tccgttttca gtaactgtaa gatttgtcaa
     1861 ttctttcaat gtaagatcct gtggtgttcc atttacccct gttaagtgac gaagatttgc
     1921 atcatgcatc actacgaaat gaccatcttt tgtttcctga acatccatct ctacataatc
     1981 aggatgatag tctctgcttg ttttttcaag tgcttctaaa gtattttgcg ccccattttg
     2041 ggtactcaca cctctgtgag agacagtcac tggttttgtc tgaacagctg aagtcagata
     2101 atccatattg taaagacaga cgccaatacc gaaaaaaact gcaaaaagag tcaaaccagt
     2161 gtttttcagc gcagagaaac gcaagttggg ggcttgtggg ataaaccact tagggatctc
     2221 aggcaagaag ccttcgtcat ccatgaaatc aacaatgata taaaaaatcc ctacggtaga
     2281 catcacgata ttgaataata aaatcccttg taaaagcgtc atagcaaaca cagcgctgat
     2341 caatgcatag tctgggaaaa attgttcaac catactttgt gccaaaataa caacgatata
     2401 tcctaaagaa gaaaggagta aaatcgtacc gccaatcaca ataaattggc ccgtgatggc
     2461 caaaagacgc gatttggtca atgaccagct ttcccgaatg gctgctcgaa aaggacgatc
     2521 tcgtagaatc atttccggta aagcaaaaat cagtcgaatc ccaatatagc caaggaaaat
     2581 ataaacgagt aaaaaggaac tgacgatgat ccaacgatta gcaaaaataa aatccataat
     2641 gaaagcgggg atttttattt ttgacagtag atcagaatta aaacttaatc cgctgatcgg
     2701 tagaatcaat aaaaaataag caaggaaaaa caaaaaagta atgggtctca ctttcttcag
     2761 ctgaagtatc gtcaagtgta agagttgctt caaagagatt ggttctttct ttttaataaa
     2821 aaaaacactc atcaaaagaa aagtaaattc gaaataaact aacagtaaaa tcgtcatcaa
     2881 aactacgatt aacgagagta gaaccccagg atgttgagaa aaaattgaag gaatattgtc
     2941 ataagacaaa tagtcaattg cccctcggtg taaaataaaa cgggtcgtac tgctcaataa
     3001 gggtaaacaa ataaataaaa taaagccatg catcagcaag acatcacgga aataagcttg
     3061 tgttcctttt aaaaagtccc aagcattacc taagctacgt ttcaaataat tcatcctatc
     3121 acctgctctt cacattgtag cgcaaatcta atgaaaaagg aaagggtgca gtgtgttttg
     3181 cagacccttt cccagattta acattgtcaa tcaatgacta attttacaaa ataaatatta
     3241 tccaagaaga ccacttagat attgacctcc ataaatcaag gccatactat ataagaaaat
     3301 cgacagtggt ttagcaatca aaatgattag tgtatatcgt ttcactgaca tgtttgtcaa
     3361 tccagccatc aaacataacg catcgtcagg agcaactgga agaaaaatag ctagagcaaa
     3421 cagcttctca aaacggtttt gattatccag ccagccaata tatttgttat atgtcttatc
     3481 gctaatcatg gaaagtatga atggctttcc ataacgacgc cctagcagaa aaatgataat
     3541 cgaaccaatg cagattccaa tatagttata gataaatcca gcataagggc caaatatcag
     3601 gacaccagct gcagtactga tccctccagg aatgattgga ataactactt gcaggatttg
     3661 gatgaagaca aaaataatcg gtcctaaaat gatggaatca cctactaatc ctcttaacgc
     3721 attcaaatct ttgaaaacac ctaaattaat aaaataaatc gtcagcgcga ttgataggcc
     3781 taatccgata atggatatga aatttatcag ttttcgagag gcagcaatat tcattattca
     3841 tcaactcctt agaggttctt tgttaactat actttactag aaaagcaact aactgcgcca
     3901 ttttttgata tgacagaaaa caaacaattt tgtaagttca tcataagtat aaaagattga
     3961 tctcaaaaaa acatccaact aaagacttat ttaataactt g
//
LOCUS       DAZKLA010000178         3990 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000178 DAZKLA010000000
VERSION     DAZKLA010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.182"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            527..2020
                     /locus_tag="ACG85N_002714"
     CDS             527..2020
                     /locus_tag="ACG85N_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0225419.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            2155..2451
                     /locus_tag="ACG85N_002715"
     CDS             2155..2451
                     /locus_tag="ACG85N_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFQ0225420.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            2552..3403
                     /locus_tag="ACG85N_002716"
     CDS             2552..3403
                     /locus_tag="ACG85N_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225421.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            3424..3636
                     /locus_tag="ACG85N_002717"
     CDS             3424..3636
                     /locus_tag="ACG85N_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225422.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         1417 a          578 c          805 g         1190 t
ORIGIN      
        1 ataagaattc agactttata aggaatatag tggatatact aaatatattt gctatattca
       61 gtatatcaga tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag
      121 agtgaaaaga ggtaaactta attctataga aaccacagat aattcactat ttacaaagta
      181 gcaaaagcag tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat
      241 tgattttagc agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt
      301 ctcaagaata tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc
      361 cgattgcctt tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt
      421 gtacttaaat taaatattct taaacactga agagagaaca catactatgg ctgagattaa
      481 agctaaactg tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat
      541 ttattcagct attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa
      601 acctgcaggt catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca
      661 attaatgaat gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga
      721 aaagaaacta acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta
      781 tcgaacagtg gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt
      841 tgatatcgat tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt
      901 gggctacatg ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact
      961 aaagacacca ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa
     1021 aatttcaaaa acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg
     1081 taaccacttc ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa
     1141 ctatcagtat tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa
     1201 aacaaaagaa gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca
     1261 agtagatgaa ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg
     1321 acgtcttggt cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta
     1381 tgcattggat acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct
     1441 atctgaagca gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc
     1501 aagcagagaa ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa
     1561 tgatctgttc attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa
     1621 atatagtcat aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct
     1681 tgctagtgag ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc
     1741 caagcgaaca cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa
     1801 ggtaacacga ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag
     1861 ggttattcaa ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt
     1921 tggactcaac gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc
     1981 tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg
     2041 ccctatacaa tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt
     2101 aggtggtata cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg
     2161 ttaaaaaatt atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta
     2221 tctgtggaag tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt
     2281 accagggaaa aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct
     2341 tctggggaga ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt
     2401 gagtataaaa tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta
     2461 tttacttaaa aaattggttc attgagcaac ctatcgctgc taaagtagca aaggaaaacg
     2521 ttaaaggaaa gaatttaggg aagaaatcga aatgaaatcg gacgactgga ttgaacacac
     2581 ctggcttcct ctgaaaagaa atagcaaacg aaagaaattt tgggtattgc aattatggcc
     2641 attaggaaaa ctttgtagtt acggggcacg acttattctt gatttaaccg tttactcgat
     2701 tggagtattt ggaacggctt attacttgat aaatgatccc aataggattg cagcatcaga
     2761 catttttaca gtgctaatga cgttgatttt tgtcatgcta ttgtctattt ttactcaatt
     2821 agtcatgcaa tttaccaggc aaaaattaac agcaggaaaa cttttcaaaa gcctattcga
     2881 tttaattgtt gtaaatgcaa ttctagcgac tttaataaaa caaattgaac aatctggaag
     2941 aacaacaaaa ggagtgaagg attttttatt gacgtttatt cactttgtaa atggacacat
     3001 gtatttgttt gaagtgggta ttattctctt ttttgtcggg tatgtatcca cgaaacttat
     3061 taaacgttca attgaaaaag aaattgaaat tgacctgatt ccgacacttc ttggttatga
     3121 gtacaatgtt gattttggaa gatatgacga attgatcaat acggatatcg ttcacacgag
     3181 tatccatgaa tctactgtta agggcatcag atctatttta aaaaatggcc aggaaatgaa
     3241 agcaatagat cgaagaaaag agatctacaa tttttatgaa gtcgaagcaa gcctacgata
     3301 tggcatcgga aaattgtggt tagatggaag taatattcgt caaaacaaac catacatttt
     3361 ttggaacagt agtgatttag aacttccaat gaaagataaa taattatgga gaatcgtttg
     3421 aaaatgattc ctctatacag gattgtgctt atagttggca tggagttaaa aaccttaaag
     3481 aaggagcagt tattcaaagt gaaagaacgt cataatcagt tactatgtag taaaacgggt
     3541 acgatgaaca ttgaataccg aacagaatat ttagaattaa cgaatcaaac tgattttttg
     3601 ttgattcgaa agcaacaaga gcaagaaaaa aattaatggc tacttatagc caattgattg
     3661 atgaatggga acaattcaag gaatctaaaa ttccagaacg tctctaaaag cctttatttg
     3721 taaagagcat aaggaaacgt gggaacactt tttaaaaaaa gtgttccaat aggctgtgcc
     3781 tattacgttt ctcaaaaata aatgtgggaa caaaatattt aaaaatagga tagtgaaagg
     3841 atagtgaagg atagtaaaag gagggtagta ggatagcaaa aaggataggc tccttttcgc
     3901 tatccttttt aaattggagg aattaatatg gaagtaagaa taagaaatgt ggatgagaaa
     3961 atcgtaagag aaattgatcg aagatgtgac
//
LOCUS       DAZKLA010000179         3969 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000179 DAZKLA010000000
VERSION     DAZKLA010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.183"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(403..1350)
                     /gene="arcC"
                     /locus_tag="ACG85N_002718"
     CDS             complement(403..1350)
                     /gene="arcC"
                     /locus_tag="ACG85N_002718"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFQ0225423.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(1465..2484)
                     /gene="argF"
                     /locus_tag="ACG85N_002719"
     CDS             complement(1465..2484)
                     /gene="argF"
                     /locus_tag="ACG85N_002719"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFQ0225424.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(2575..3804)
                     /gene="arcA"
                     /locus_tag="ACG85N_002720"
     CDS             complement(2575..3804)
                     /gene="arcA"
                     /locus_tag="ACG85N_002720"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFQ0225425.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         1072 a          846 c          692 g         1359 t
ORIGIN      
        1 tgttcccttg attgttaaac aaaaaaaatc tctagcgacg gcatacttag tatgccatca
       61 ctagagacga gatttcccgc ggtaccactc caattgtcct aaaaaaggac cagctccatc
      121 tgatatcggt agacgaccgt ctgtttaaaa cagaattctc caagtgcgct tcgttgtatc
      181 ttcttcattc ggcttccacc acttccgaat tcgctagcca agaatgatac aagtactctc
      241 ttgttcaaca aatcttagtc ttattaaact ttcttgaatt atatcagaaa aaaaaacgaa
      301 aggctagtcg tttcataaaa aaaaaatcca gatggcctaa aggagaagaa ataccacctg
      361 gatagctata aatcatttcg taaatacgaa agacttaaca aattaatcag ctacgataat
      421 cgttccttct tcagaagcta aaagattctc gatattttct aaagaagtga tgattgcctt
      481 agcgtttggt tttgcttcaa caaatgcaat agctgcttct actttcggaa gcatgcttcc
      541 tggagcgaat tgcttttcat caatgtattg tttcatttct gaaacagtaa cagtttctaa
      601 tttcttttga tctggttttt ggtaattgac atacacattg tctactcctg tcaatacgat
      661 cagcaaatcc gcatcgatga tttcggctaa tttttctgaa gcaaaatctt tatcgattac
      721 ggcttctcgt ccttctaatc cagtagctgt ttctacaaca ggaatcccac cgccaccaac
      781 agagactgtg ataactcctt gatcgaccag cgtttcgatg acacgtgctt ctttaatcga
      841 gatgggttta ggggaagcga ctacttttct ccatccacgt ccagcatctt ctttgaatgt
      901 cacagtggag tcagcattca tttgttcttt ggcttcttct tccgtgtaga atggaccgac
      961 tggtttactt ggatttttga atgaaggatc attttcatcc acaataactt gagtgactaa
     1021 agaaaccaca tctttatcga tccctttttc cttcagtact tcgcccatgg cgttttgtaa
     1081 ccagtaaccg atagatcctt ctgtcattgc aacacatgta tctaaaggca ttgccggtgt
     1141 tttctcagaa tcagcagctt gctgctgaat caataggtta ccgacttgcg gaccatttcc
     1201 gtgggagatg atcaattcat ctccttgttc gataaatttc actaaatact ttgctgtttc
     1261 cattaacgct tcttgttggg cttttgcact tgcatccgta gacaagatgg cattgccccc
     1321 taaagcgaca actacttttc gtttggccat ttcattcgtc ctttcttcag ttctaccgaa
     1381 ttttctagat aacaaaacga gccagcgttt tgtgattttc cctcaatgct gactcgtttt
     1441 ttcgtgagat gaatgtggtt atgcttaaac gcgagggata aacaagttac ctaatgttgc
     1501 tgccatgatc gctttgattg agtgcatacg attttctgct tggtcaaatt gacgagcata
     1561 tttgctgcgg aagacttcat ctgttacttc catttcagtg atgccaaagc gttctttcat
     1621 ttgttcaccg taaacagtgt ttgtatcatg gaaagctggc aagcagtgca agaagatcaa
     1681 acgatctgtg ttgtgtgttt tttctaccat ttccatattg atttggtaag gtttcaacaa
     1741 tttgatacgt tcttcgaatt tatcttcttc acccattgat acccatacat cagaataaag
     1801 gacgtctgct ccgtctacac ctttatcaac atcgtcggta atcatcaatt gtgcaccgga
     1861 tttttcagca aagccttctg ccattttgac gatttcttct tcaggttgta attcttttgg
     1921 tgcaacgata cgcatattcg ctcctaaaat tgcacctgtg actaataaag agttagccat
     1981 attgtttcga ccatctccgc agtaagcaac agtgatacct tctactgttc caaagttttc
     2041 ttggatagtt aaaaagtcag cgatcatttg tgttggatgc cattcatcag tcaagccatt
     2101 ccaaactgga acaccagaga attctgctag ttcctctacc attttttgac tgaatccacg
     2161 gaattcgatt ccgtcaaaca tacgtcctag tactttagct gtatcttctg tggattcttt
     2221 tttacctaat tggatatcat ttgcacctag atattcagga tgtgcaccta gatcgatcgc
     2281 tgcagtagta aatgcagaac gagtacgtgt agatgttttt tcaaataaaa gcgcaatatt
     2341 ttttccttct agatagtgat gtgggatacc acgttttttc aagtctttca agtgttctga
     2401 aaaatcgatc aaatattgta attcttcttt tgtaaagtct ttttctgcta ataagcttct
     2461 tccttggaat actgattctt tcatattaaa cattctcctt tagattgttt ttatttaaga
     2521 ttaaagagga aatcccgacg gcagctgaca gtaagactgc cgtcaggaaa caaattactt
     2581 caagtcttca cggacaagtg gttggctcat gcagcgtgga ccaccacgac ctcgtgacaa
     2641 ttcacttgaa ttgatttcta acactttgat gccatagcta cgaagcaatt cgttcgatac
     2701 ataatttcgg ttataagtga ctacaacacc tggagcgatt gccaatgtgt ttgatccatc
     2761 attccattgt tctcgaggtg caacgatctc atctccatta ccagttggga tcagtacaag
     2821 atcatctaat ccaagagcag ctttcatcgt ttcgtgtaag ttatctgaat gagtaatctt
     2881 gatatcatca ccatctggtt cgattgtaaa gacatccatc ttaccgtctt tactttgaat
     2941 tgctggatgg atagtgaatt tatcgtgatc taccattgta aatacggtat ccaaatgcat
     3001 cattgcacgg ttgttcggaa ttttgattgc caacactttt tcaaatcctg aattttttgc
     3061 gaataggtgg cgtgctaatt tttctaaagc tttcgcatta gttcgttgag aaatcccaac
     3121 agctactact ttatcgctta agatcaattc gtctccgcct tcgatatgat ctggattttc
     3181 acgatccaac catacttcga ttcctttatt tgcaaatctt ggatgatgtt tcaaaatata
     3241 ttctgtaaac atcgattcac gacgacgtgc ttcaaaagtc atcttattga ctgtcatccc
     3301 atttcccatt gatgcggaag ggtcgcgtgt aaagtacaag tttggcattg ggtccatgta
     3361 aaatggatag tcatcgtctg ctgacagatc atatagactg ctagaacgta cttcgatatc
     3421 ttttgtacga accccagcca tgattttatc taccatttct tgcgtttcca ttgatagaag
     3481 gaactcatga agaccctctc ttacagcatt cgatgcaata tgagattcat ccaacatttt
     3541 attcaagaat tgttctttga cattgcctgc gtcaatcgct tctgccgcta atttttccaa
     3601 gtaaagagtc tcgacgcctt cattttgcaa tgtcttcgca aagttgtcat gttcttcttg
     3661 agcaatcgga agatatggaa tatcatcgaa caacagacga tccataatat ctggtgtcaa
     3721 attttccact tcttgtccag gccgtttcag taaaactgtc tttaatttcc ctatttcaga
     3781 gaaaacgtga ataggtttat ccatgttgtg ttgcctcctt ctttttgatt acatgtatag
     3841 gatatcctcc ttttgtaatc actttcataa ttttgtctca ttcaaaatga gacattcctc
     3901 acattaatat tcgataatta aataattaaa cgtttttcta tttttaagcg taatttattc
     3961 attttttat
//
LOCUS       DAZKLA010000180         3963 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000180 DAZKLA010000000
VERSION     DAZKLA010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.184"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(69..611)
                     /locus_tag="ACG85N_002721"
     CDS             complement(69..611)
                     /locus_tag="ACG85N_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFQ0225426.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            746..1135
                     /locus_tag="ACG85N_002722"
     CDS             746..1135
                     /locus_tag="ACG85N_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225427.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1139..1903
                     /locus_tag="ACG85N_002723"
     CDS             1139..1903
                     /locus_tag="ACG85N_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0225428.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2032..2979
                     /locus_tag="ACG85N_002724"
     CDS             2032..2979
                     /locus_tag="ACG85N_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0225429.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3109..3849
                     /locus_tag="ACG85N_002725"
     CDS             3109..3849
                     /locus_tag="ACG85N_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225430.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1312 a          675 c          805 g         1171 t
ORIGIN      
        1 ataataaata aaaactgtct aacatttaaa atttaattta gatattagac agtttttaag
       61 tttgtgagtt attttccttg tcgttttctc acttttttag tccagaaacc acctgaaata
      121 tattttggct cataagaaat gataaaagca cgctcttcga tttctttgat caggcgatac
      181 agttcacgtt cgctttttct aggagaaagt atttcgagaa ctagccgctc accttccagc
      241 cccattgcag aagtctgtgt cacaccataa ccgtgttctc tcagtatacc cggcaaactt
      301 ttatcttctg cgctatttgc tggcaaaatc gttgtgacca tgatgtatcc taatgccaat
      361 ttgtcttcaa tcaaaattcc gacactgata cctactgcgt aacctaacgc atagacaaat
      421 aaattcagtg gattatctaa acgatctaaa acaagggata agccgacgat ataaatcgtg
      481 atctcgacca tactgacaag tggagcaatc agtcgatagc ctttcattgt caacatgaaa
      541 cgtaacgtgt tcagagtgat ataagcaaaa ttaatgagga agatcatccc caacattttt
      601 ggatcaatca tttgtaaaac tcctttcatt tatataacaa caatatcaaa aaaaatcgta
      661 agtgaagaaa aattgaaact ttatacatac aaaaaaatga taaaatgatt ataatagaat
      721 tataaagata ggttaggtga ttaaaatgaa aacggtcaat cgtgtgtttt gtgccagcca
      781 cgtgctaagt ctccttctca gtatatactt aggtgtctat tcgattcaac agccaattga
      841 ggtagagaac attgtttttc agatgtcgct gacagatggt ttaattttaa gcgttttttt
      901 ctttttgata ctgtttattg gtaatatttt cggtagcgtt gccagttttt tgaatttttc
      961 tatttatcca ctcctttcgc tagcattagg agtggtggga ctttgttctc tgtgcttatt
     1021 tcccgagcca ttcagtcctg catttatatt atttggtaca ctgaacctgt tccagacaac
     1081 tgttggagca tggttgctat ggcgatcagg aaatttaatg aaaataggtg agtaacaatt
     1141 gaatccaatt atattgaaac gttctgtcca tcatattcca gtgttagaag ttgttctgaa
     1201 agaattaaga gaggaacgta ttcccgttgt catctattat cacggatggc agacaagtaa
     1261 ggagttagtt ttgacgcaag gaagaaaaat tgcgcagcag ggcattcgtg tgatcctgcc
     1321 agatgcgatg aatcatgggg agcgcaaaca gccggtttct tctattccat cctatacatt
     1381 ttgggatagt atctacggaa atctgtttga atttgacaca cttatcaagc atttagaaaa
     1441 aagaggtctt ctacatgatc agcttgcagt tggcggggtt tcgatggggg gaatgacaac
     1501 atgcgccttg ttggctaaac atcctcatat agtagcgggc gtgtgtttga tgggttcccc
     1561 tgcacctttg gaatatggaa aagaaatcgt aaggcgcgca tcggaatata attatgtcgt
     1621 accaaaagac tattttgatt tgatttcatg ggttaagagc tatgatttgt cattgcagcc
     1681 ggaaaagtta gctggacgtc ctctgttctt ttggcatgga caagctgacg aaaaaatccc
     1741 ttatatacaa acggaacgat ttgtagaaaa aaatcggcat aagtcttacg gaaaagaaat
     1801 tgtttttcgt tcctcaaaaa cacagcgaca tatggtacaa ataccactaa tggaagaagc
     1861 ttccactttt ttagcaaaaa atttgataaa aaagtataaa taattgaaac ggaaaatcac
     1921 tcatgacgat agaggatttt ccgtttttta ttttgggcta taataaactc tgttaggaaa
     1981 atttggtata attgatagta agaatgacaa catgttagaa aggaagagag catgcccact
     2041 ttagcagatg ttgcaaaacg tgccaatgtt tcaaaaatga cggtctctcg agtcatcaat
     2101 catccggaac aggtgactga cgaattgaaa gagttagttt tctcagcaat ggctgaactg
     2161 gattaccgtc caaatattgc tgcaaaagca cttgtcacga atcggtcgca gatcatcaaa
     2221 ttatttatac tggaagaaat cgacacaact gagccctatt acatgaattt gctgatggga
     2281 atcgctaaac ggatcggtaa agcgcattat tctctccaat tagttacaaa tgatgccttc
     2341 gacgtcggtt cttgtgatgg atacatcatc acaggtgtca gaaaaaaaga cttcgattgg
     2401 atcgaccgtt tggaaaaacc agttgtcttg tttggggaaa attctgaagg atatgattat
     2461 gtagacagcg ataatgaata tggtacagca aaagcaacgc aatatgcctt agaaagagat
     2521 tatcaaacga ttatttatat cggtatcgaa gtggacgagc cttttgaaat ttcaagggaa
     2581 actggttatt tatctgtgat gaaggaagtt gatcttccta gtaaaatctt gcggtttcca
     2641 aatcattcga cagaagtaga aagatatatg acagaaaact gggcagattt tacagataaa
     2701 acttgttttg tatgtagttc agatcggcta gcactaggag cagtaagagg gattataaat
     2761 gctggaggac acttgcccga agaatttggt gtgattggat ttgatggggt atttttagac
     2821 caaatcagtt cacctaaatt gacaacgatc aagcaagata ttatacgtat gggtgaagta
     2881 tgtggagaaa tgttgttaaa aaaaatagaa gaaaaagggg cgagtcaagg ttatcgtcgt
     2941 ttttttcctg aactagttat cagagagaca acgaattaag tcttattact atatatatat
     3001 ttatgaaatt catcgctatt ttcttaaaat aatcagcgca ttgctactga aaacaatgaa
     3061 tctgtgctat tattgagaaa agttccattt ttgagaagga tggtttgaat gaaagaaatc
     3121 agacgaaaac atttccgttt tcctgcgtac gatgatgagt taggcgtaaa attaaccaac
     3181 gacaagaagc gagtattatt tgacggacaa gaagatcttt tgactgaaca gaatcagtcc
     3241 ccgtttgaac agatgaaaga atttcatttc acgaacacga acaccgagaa aatccgtcgc
     3301 aagcgtcgtg caacaaatgg attgaagtca gctccgcgta aggaaaattt ggaaaagcat
     3361 aaaggaaaat taccggatta taccggccat taccaatcag aaatcacatc gacaggacga
     3421 aaaaaacttt ttggtgattc ctctcgttct gtcagacaaa gtgaaacaag gacagagtcc
     3481 aagcatacta cacgaaacgc atctgttgac acgaaggtgc gggagcgttc ttattttgtt
     3541 cctaaatatg tacccgcatc gattatccca gaccctagag aaagcaaaat ttctgaggat
     3601 catttaatcg aatcgatgaa aaaaacggat gactcttatc tgttgttcga tacagaaccg
     3661 acgccgtatc aagaaaaaag aaatggtgag ccgtctgtac gaacattcaa tcgaacaaaa
     3721 acgttagagg aaacaaagca gtcagaagaa aaaaaatcga aaaaaagcca tggtatctta
     3781 gaacgctcat tacaaggatt gatcgatgat caaggaaact ctttgggaga aaatagctat
     3841 tttaaataag tgatatacaa atttatattc ggctacagtt ggaaaggaaa cgctgcggcg
     3901 tttccttatt ttatactcta tccgaaagtt tatatcgtat cctcaaattt ttttgagttt
     3961 aag
//
LOCUS       DAZKLA010000181         3962 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000181 DAZKLA010000000
VERSION     DAZKLA010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.185"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(612..1169)
                     /locus_tag="ACG85N_002726"
     CDS             complement(612..1169)
                     /locus_tag="ACG85N_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225431.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1348..1959)
                     /locus_tag="ACG85N_002727"
     CDS             complement(1348..1959)
                     /locus_tag="ACG85N_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225432.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2072..2329)
                     /locus_tag="ACG85N_002728"
     CDS             complement(2072..2329)
                     /locus_tag="ACG85N_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225433.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2926..3204)
                     /locus_tag="ACG85N_002729"
     CDS             complement(2926..3204)
                     /locus_tag="ACG85N_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225434.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3238..3489)
                     /locus_tag="ACG85N_002730"
     CDS             complement(3238..3489)
                     /locus_tag="ACG85N_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225435.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1200 a          811 c          526 g         1425 t
ORIGIN      
        1 tcttatttct tggagttaaa cgcttctgtc ccagcctcct tattccgaat aaatggtgtc
       61 caaaagcttg catctgccag attatctcat ttcttatgac aagctaagca aattatctcc
      121 accaaataat ttattcatat ttttgacttg cgcatactaa ctaattctta aattttcata
      181 caaaatttct caatttccct taacgagtaa tatttatgta cggtgattct cactagtatc
      241 ccaatcactt tttaagttat cccgatttca attctacttc aaaaatagtt gcttttatgt
      301 atgctgttag ttgattagat ggtcatttgt tgagctaggt cgtttatttt aaacaagttg
      361 tttctttagc ttatacctat ctcgtctacc aaacgcaaga tggcaaacac aaatgcatac
      421 gcatctgtat ttgccacttt gattcgtata aagctaacaa ctattaactc ctctcagcta
      481 atagttgctc tcttttttat tcagattcat ttttgctgat cacagcctag tatttcaatg
      541 gttttgaaca ctaaaccaaa ttgttccatt acaacattca ctggctgatg aaatggatta
      601 gtagatttcg attattcaaa gcgattttct attggtggat cgtgttccca actaataaga
      661 cggattccaa acctatcgta cagatcttct aaaccactta taaattttgc acctatttca
      721 ggtgttttat tgcgtggata ttctattgtt acgccattaa atggatacca attaggatta
      781 ggctctcttt ttttctgtac atctcccata gaatagttag cactttctgg gacataatat
      841 attcttcgac catcctctaa ttcatctaat aagatatttc gtgatcgtct atcacttgct
      901 aaatgtagac gccacgtgcg ataccatgcc atgctacctg catctgcaga aaatccatca
      961 aataatgcaa caattgtatc ttctatcaca atcctttccc catctacttc atattctaat
     1021 ctaaaatcaa actcccctcg ggtaatttct ggtcttggtg gcttaggacc caatattatt
     1081 ctacttaaaa ccgcattcgt tcccagatga aacacagcta taacaacaat aatgattaat
     1141 attttgtttc tatttgataa atttctcatt attcgcacat cacctctaca ttctcacata
     1201 ttagttcgta atactccttt aatatcaaaa tatctactgg tcgtaataat attcgctatg
     1261 aattcctact actcgtataa gaaaggccaa atcatcagct tttacttgct gatgatttgg
     1321 cttgtttcta tttatcgtaa ttgttttcta atcaccaaaa ttgttaacaa tcggttcaga
     1381 tatttcccag ttgattaagg taattccgta ttgtgtcaaa acttcttgcg cttctccgaa
     1441 attaagtaca gttcttatgt atccactttc tcctaatata aagtctacct ctcttttaaa
     1501 accttcctcg agatttctaa gggctatatg tggttttaat gacattgatt gtacattttc
     1561 ccccatataa tattgcgcac ttcctcgact aaaatagatt cctagatcgt ccccaaaagg
     1621 tagatcttct ctaccactag ctaatctact tctccatctt cgttctcttt tcatagaacc
     1681 tgcgctgaac tcacacccgt taaactcagc tatgacagta tcttcaatta tgttaatctg
     1741 tccatctagt tcgtattcaa tcctaaatgg aaattctcca taggtgattt ccggttcggg
     1801 tattttaggt gagattaatg caaagaaagg aaatattaag cttggcaaca tcaaaaaaac
     1861 tcctcctatg atcacaattc ccgatgtaat ccaaaatata atttgtacaa ttctcattga
     1921 tccattgtat tccattttat tccccctatt actattcata tccgcctatc tatatcgatc
     1981 ttccaatgca gagataattg cttccatacc ttgatttttt actgagttac ctatgcgaga
     2041 tatcattctc cactaaggtg catcatattg tctatctaac atgattaatt ctccaatagg
     2101 tgtatactcc tccaaaaaca acatttaatg catctccctc gacaacataa gctaccatgt
     2161 ttgctgtata tgtgtttgag tctaattcat aagcagataa atttactgtc gctggattaa
     2221 atataatcgc attcgtattt atagctactg aatttgctgc ttcagcacca ccttttgaat
     2281 gtccgatcat tgtgatttca atagtaggat ttacgtggac agccctcacc gtttaccctg
     2341 accattatag aaatcgctta cttttttgaa ctacgcaaca aaggatacaa tgctcctaac
     2401 ttcactgtct ccatttttgt ccactaagga atataaaaaa taggcattaa ttcatttaat
     2461 tcggtcacaa cacgttacct tttaaatcat tatactgtat ctcatacatt attatcaata
     2521 aactattata aaaatgcaaa aaatgcttta tcaatctatt ttgtaattgg tcataatatc
     2581 attgaaatca taccctgtat cgatttttcc tatgaatctc gtctatttgt gattgacgaa
     2641 aaagaggcaa gacagacact ggaatggtaa ttttacccac tattttgctc caactatttg
     2701 taatcgtacc cacttttgaa ataccgagat gatcatttta attgatatgc tgattcccaa
     2761 gcaaaacccc aagaaattaa atgctctatc gcatctagct tcttgggttt tgcttatata
     2821 aattccaaca tttaaatttt ttatgctgtt cggtctcaga aatgccgttt ttgcttatat
     2881 cacaatatca agatgtcaaa ttattcgtgt tgccgatgac tttgattaat ttttttcaaa
     2941 ttgtttatta agactaatgc tgtctttttg cagataccaa tgttcgaact ttttaacatt
     3001 atcatatgat cctgcaagtt ttaacttctt accattagat gttatcaaag ttataattaa
     3061 ccttgccttt ttcccattag gatcttttat tttatgaatt ttctgcaggt aatctctcct
     3121 aatggcagct atttttacct catcagtgct gataaaccat tgatcatcca catacatact
     3181 gcctgttatg ttgtgttttt ccatttcttc cgagaacgaa aagtttaagg ctcgttctta
     3241 cttccaaagt tgaatgagtc catggattgg acttagtgtg agaattgaga acaacaataa
     3301 gaatccatat cctcccgcaa acatccctaa ggctatactg tctctccatc cataagtagg
     3361 attagaagag aaaagagtca ccgtttgtaa taatatgaaa aggaataatc cagcacaaac
     3421 accaaagata acaactccaa acattgaatt tttatacaat gaccatttat ttataggctt
     3481 ttctttcatt ttacttcccc ctactgttct gttcacacgg aatgaattca aagttcataa
     3541 gccctcctct ttgaacatat gaatacgact tgctaactat actttaaaaa agcttgtttt
     3601 aagcggttat aggcgagttg taatctctat tcatctcatt agcaaataac ttgccgaaaa
     3661 gtctactgaa attaaagcag atagtggaag aattatttag ccaggataga cactatttgt
     3721 gaaacacatg tctttacttt tgaaattcta tgatgaaaag ccttggaata cagtagctct
     3781 taattggtaa tactttttcc attataaata acctcttttc attttatatt tatcccctct
     3841 aaaaataata tctaatctaa tattttaaac ttatatttcc ctcgctgtgc taatagagca
     3901 ttactaaata aatagaaact agaaaaaagc attacgcctg ccagaataaa ccaattattt
     3961 gt
//
LOCUS       DAZKLA010000182         3847 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000182 DAZKLA010000000
VERSION     DAZKLA010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.186"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..1155)
                     /locus_tag="ACG85N_002731"
     CDS             complement(1..1155)
                     /locus_tag="ACG85N_002731"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFQ0225436.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1340..3781
                     /locus_tag="ACG85N_002732"
     CDS             1340..3781
                     /locus_tag="ACG85N_002732"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFQ0225437.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         1231 a          721 c          840 g         1055 t
ORIGIN      
        1 ttatattttg tagtaacgaa tatcattttc gatctttgta atcgaaaaag gtacatctga
       61 agtatattct gcacattctc cgtgttcagg aagcgtctca ctgaaaatcg tttcatattc
      121 gtcaacagtg atccgtgttc gagaatctag catctcttga tgagattgtg cgaagagata
      181 ttcttgatag ttttcttcca gataacctgt aaaaaattca cttactgcac cagaaccgta
      241 gctgaataga ccgatgcgat ctcctggctg tagtgatttc gaattttcca gtagagaagt
      301 tagccccagg tataatgaac cagtgtaaag attaccgatt cgtcggctgt aacggatgct
      361 ttcttcatag cgagccataa gacgttcctg attttcttcg tctgtttggt ctaatacgct
      421 ttgcaatgcc ttttttccca tcttagtata cggaatatgg aaagcaatcg cctgataatc
      481 ttcgagtcct cgacccgaca attctttatg tcgattccaa actttttgga atgattcgat
      541 atacgtagaa ttagataaag gaccatcaac aacaggaaat tcgctataat ctggacgcca
      601 gaaatcatag atatcttctg tcagaaatac gctgtcgtct tcaatcgata aaatacgcgg
      661 gttttgagtg atcatcatcg caacagcacc gacaccttgc gtcacttcac caccgcttgc
      721 caagctgtaa cgagcaatat cacttgctat gactagtact tttcgttctg gatgattttt
      781 gacatattct ttcgccatgt gcaaagctgc ggtgccaccg tagcaagcct cttttacttc
      841 aaaagaacga gcaaaagatt ggattttcag taaatggtgg ataattaccg cactcgcttt
      901 tgaatgatca atcccggatt ctgttccgac gatgaccatg tcgattagct cacggtcttt
      961 ttctgttacg attttgctgg cagcatttgc tcctagtgta atgatgtctt catttgcacg
     1021 attgactgcc atttggtctt gaccaatccc tatatggtat tttgcaggat catccccacg
     1081 gctttctgcc aactctgtca tatctaaata taaattagga ataaaaaagg aaagacgatc
     1141 aatccctatt ttcatttcaa cacctcttta cttatgataa aaaacttact ttactaaggt
     1201 atcataaaaa gattaaaaga aatataaaaa agagaatatt taaggattgt taaaatttaa
     1261 tcttagaaaa aaataactgg aacaagagct atttgttttt acagcaaaga atggtatatt
     1321 tttgtaggag gtggcttact tgaaagaagt cgttatgata gatgctgcaa gaacgccgat
     1381 aggaaagtat cgcggaagtt taagtccatt tactgcagtt gaattaggca cacttgtgac
     1441 taaaggatta ttagataaaa caaaactgaa aaaagataaa atcgatcaag taatatttgg
     1501 aaatgtcctg caagctggta atggtcaaaa tgttgccaga cagattgctt tgaacagcgg
     1561 tctccctgta gatgtcccag caatgacgat caatgaagtt tgcggatctg gtatgaaagc
     1621 agtgatctta gcacgccagc tgatccaatt aggtgaagca gaacttgtga tagccggcgg
     1681 aacagagagt atgtctcaag cacctatgct gaaacagtat cagtcagaaa caaatgaata
     1741 tggtgaacca atttccagta tggtcaacga cggattgact gacgcatttt caaatgcaca
     1801 tatgggatta accgcagaga atgttgcaac acaattttct gtgagcagag aagagcagga
     1861 tcgctatgcc ttgtcgtccc agttgaaagc agcacatgct gtcgaagccg gtgtattttc
     1921 tgaggagatc atcccagtca agatttctga tgaagacgtg ttatctgagg atgaagcagt
     1981 tcgtggaaat agtacattgg aaaaactggg cacgttacgt acagtattct cagaagaagg
     2041 aactgtaaca gcaggaaatg cttccccgtt gaatgacggt gcctctgtgg tgatccttgc
     2101 atccaaagaa tacgcagaaa ataataatct gccttattta gcaaccatca aagaagtagc
     2161 ggaggtcggt attgacccaa gtatcatggg aatcgctcca atcaaagcca ttcaaaaatt
     2221 gacagataga tcaggaatga atttatcaac aatcgatctt tttgagatca atgaagcatt
     2281 tgcagcttct tctatcgtag ttagccaaga actgcagtta gatgaagaaa aggtcaatat
     2341 atatggagga gcaatcgcat taggccatcc aattggtgca agcggtgcac gtatcttgac
     2401 tacattagct tacggattgc ttcgtgaaca gaagagatac ggtattgctt cattatgtat
     2461 tggtggaggt ttaggcttgg cagtcttgtt agaagcgaat atggagcaaa cacataagga
     2521 tgtgcaaaaa aaaaagtttt atcagcttac gccatctgaa cggcgcagtc aattaataga
     2581 aaaaaatgta ttgacacaag aaactgcgtt gatttttcag gaacaaacac tttctgaaga
     2641 gctctccgat cacatgatcg aaaaccaagt gagtgaagtg gaaatcccaa tggggatcgc
     2701 gcagaatttt caaatcaatg gcaagaaaaa atggattccg atggcaacag aggaaccatc
     2761 tgtcattgca gctgcaagta atggtgcaaa aatctgtgga aatatctgcg cagaaacacc
     2821 tcagcggctg atgcgagggc aaatcgttct atcaggaaaa tcggagtatc aagcagtgat
     2881 caatgcagtt aatcatcgaa aagaagagct gattctttgt gcaaatgaaa gttatccttc
     2941 aatcgtgaaa cgcgggggcg gtgttcaaga tatctctact cgggaattca tggggagttt
     3001 ccatgcatac ttatctattg attttttagt ggatgtaaaa gatgcaatgg gcgctaacat
     3061 gatcaactcg attcttgaga gtgtggcaaa caagttacgg gagtggttcc ctgaagaaga
     3121 gattctattc agtattttaa gtaattttgc tacagaatca ttggcttctg cctgttgtga
     3181 aatcccattc gaacgcttag ggagaaacaa agaaatcggt gaacaaattg ccaaaaaaat
     3241 ccaacaggca ggggaatatg cgaagcttga cccttaccgt gcagcaacgc acaacaaagg
     3301 gatcatgaat ggtatagaag ctgtagtggc agctacagga aatgatacac gtgcggttag
     3361 cgcttcgatc cacgcctacg cggcaagaaa tggattatac caaggattaa cagactggca
     3421 gatcaaaggg gataaactag ttggaaaact aacggtgcct ttggctgttg ccacagtagg
     3481 cggagcttcc aatatcctgc caaaagcaaa agcatcttta gctatgttag atattgattc
     3541 agccaaggaa ctagcacaag taattgctgc agtagggtta gctcaaaacc tggctgcttt
     3601 gcgtgcatta gttacagaag gaatccaaaa aggccacatg ggacttcaag ctcgttcatt
     3661 agcgatctca attggggcta ttggtgagga aatcgagcaa gttgcgaaaa aattacgtga
     3721 agcagaaaag atgaaccagc agacagctat acaaatattg gaaaaaatcc gggaaaaata
     3781 aaaaagcaat atctttagaa gaataaagca agatttacag ttattttatt tactgcagat
     3841 cttgctt
//
LOCUS       DAZKLA010000183         3844 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000183 DAZKLA010000000
VERSION     DAZKLA010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.187"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..888
                     /locus_tag="ACG85N_002733"
     CDS             <1..888
                     /locus_tag="ACG85N_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFQ0225438.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
     gene            1000..1896
                     /locus_tag="ACG85N_002734"
     CDS             1000..1896
                     /locus_tag="ACG85N_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0225439.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            1988..2203
                     /locus_tag="ACG85N_002735"
     CDS             1988..2203
                     /locus_tag="ACG85N_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0225440.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            2221..2493
                     /locus_tag="ACG85N_002736"
     CDS             2221..2493
                     /locus_tag="ACG85N_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0225441.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            2495..3358
                     /locus_tag="ACG85N_002737"
     CDS             2495..3358
                     /locus_tag="ACG85N_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0225442.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1553 a          584 c          659 g         1048 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc ataaagaggg cttttatata ttaatcacaa atcacttatc acaaatcaca
      961 agtgattaat cacaaatcac ttgtgatttg tgattcttaa tgatacaata ttactataca
     1021 aaaaaagaat ggggcgtagt tatggagaag gaaaaactaa aaatacttga agaattaaga
     1081 cgtattttaa acaataaaaa tgaagcaatt attatcttga ataattactt taaaggtggt
     1141 gttggaaagt ccaaattatc gactatgttt gcttacttga cagacaaatt gaatttaaaa
     1201 gttttaatga tcgataagga cttacaggca acattgacaa aagacttagc aaaaactttt
     1261 gaggtagaat tgccacgtgt caatttttat gaaggcttga aaaatggaaa cttggcttct
     1321 tctattgttc atttgactga taatttagac ttgatccctg gcacgtttga tttgatgtta
     1381 ctgccaaaat taactcgctc atggactttt gaaaatgaaa gtagattgct tgctactctt
     1441 ttagcacctt taaaaagtga ctatgatctc attattattg atactgtacc aacgccaagc
     1501 gtttatacaa ataatgcaat cgtggcgagt gattacgtta tgatcccttt acaagcagaa
     1561 gaagaaagta caaacaacat tcaaaactat atttcctatt tgattgattt acaagaacaa
     1621 tttaaccctg gactagatat gatcggtttt gttccttatt tagttgatac ggacagcgca
     1681 acgataaaat caaacctgga agaactgtac aagcaacata aagaagataa cttggttttc
     1741 caaaatatta tcaagcgaag taataaagta agtacctggt ctaaaaatgg aattacagaa
     1801 cacaaaggct atgacaaaaa agttttatcc atgtatgaga acgtattttt tgaaatgctt
     1861 gagcgaatta ttcaattaga aaacgaaaaa gaatagaatc acaaatcaca agtgattaat
     1921 cacaaatcac ttgtgatttg tgattgttga tgataaaata agaataagaa gaaatagaaa
     1981 gaagtgagtg attgtgggaa atttaggcgc acaaaaagca aaacgaaatg atacaccaat
     2041 cagtgcaaaa aaagatataa tgggagataa gacggttcgt gttcgtgctg acttacacca
     2101 tattataaaa attgaaacag caaaaaatgg cggaaacgta aaagaagtta tggatcaagc
     2161 cttagaagaa tatatacgga aatatttacc tgataaactt taaaaaggag tgaaaatgaa
     2221 atggcagtta cgtatgaaaa aacatttgaa atagagatca ttaacgaatt atcggcaagc
     2281 gtttataatc gagtattaaa ctatgttttg aaccatgaac tagatactaa aaatactcgt
     2341 ttactagaag tgaatctttt aaatcaatta gaagtggcac aagaagttga tttatttcaa
     2401 caaccatttg aagaattaca agctattcat gagtattggc ggtcaatgaa tcaatattca
     2461 aaacaaattt tgacaaaaga gaaagtggct taacatggcg aatattactg acttcaccga
     2521 aaagcaattt gaagatcgtt tagaaaagaa tgttgaacga ctaactaaaa atagactagc
     2581 ggttgaatcg ccaaccgctt ttttacttgg tgggcaacca ggatcaggta aaactagttt
     2641 gcgatcggca atttccgaag agacacaagg aaatgttgtt atcattgata atgatacgtt
     2701 caaacaacag caccctaatt ttgatgaact agtgaaactt tatgaaaaag acgtagtaaa
     2761 atacgttacc ccttattcta atcgcatgac agaagcgatc ataagccgtt tgagagataa
     2821 agggtataat ttagtgattg aaggtacagg acgaacaaca gacgttccta ttcaaaccgc
     2881 aacaatgctt caagccaaag attatgaaac aaaaatgtat gtcatggcag tacctaaaat
     2941 caactcatat ttaggaacaa ttgaacggta tgaaactatg tatgcagatg atccaatgac
     3001 agccagggca acaccaaaac aagcgcatga tattgttgtc aaaaacttac cgaccaattt
     3061 agaaaccctt cataaaacgg gcttatttag cgatataagg ctttacaata gagaaggagt
     3121 gaaactctat tcaagcttag aaacgccttc cattagtccg aaagagacct tagaaagaga
     3181 attaaatcgt aaagtatcag ggaaagaaat tcaaccgact ttggagagaa tagagcaaaa
     3241 aatggttcaa aatcaacacc aagaaacccc tgaatttaaa gcaattcaac aaaaaatgga
     3301 aagcttgcag ccacctacac caccaatacc caaaacacct aaacttccag ggatttaaac
     3361 cttaaaaaag taaaagagta gttaccaaaa aacggtaact actctttttt tataaaaaca
     3421 tttcctcatt ttttaaaagc tgatcgagaa ataaagcaca caactgtatt ctttcttttt
     3481 tcagttggta cattttgcag tctgtaacgt gccatttact gtttgttacc ttaacatatc
     3541 cagtcgaaaa cacaagggta tataaacgag caagctcgcc cttgtgtttt ctttgtgtct
     3601 atgttgcgtt ctcgtcacag caaacgacac gaacacactg gcgaaaatga accaacaaaa
     3661 cgaaaaaaga aaggagcgta cactgtaatt tatttctagc ttttaaaaca tgaggaaaac
     3721 agcttcactt cctcaaacgc ctaacaaaaa tggttgtcaa aaaactaaaa atcaaaaaaa
     3781 gaaaagagga aaagaaaatg gaaaaaacaa aaaaattaca attagaagat tttacagaaa
     3841 atgg
//
LOCUS       DAZKLA010000184         3733 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000184 DAZKLA010000000
VERSION     DAZKLA010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.188"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            482..1522
                     /locus_tag="ACG85N_002738"
     CDS             482..1522
                     /locus_tag="ACG85N_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0225443.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            2346..2678
                     /gene="traE"
                     /locus_tag="ACG85N_002739"
     CDS             2346..2678
                     /gene="traE"
                     /locus_tag="ACG85N_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFQ0225444.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3297..3638
                     /locus_tag="ACG85N_002740"
     CDS             3297..3638
                     /locus_tag="ACG85N_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0225445.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1374 a          486 c          686 g         1187 t
ORIGIN      
        1 taattttaat ggtagtacat aattattata tatatctgac attggtagta cttattgtat
       61 tttttagtag tatataatct attgttttct agcctaaagc cttgtcttat aagaataata
      121 taatatatac tactgaaata aggtagtata tattactagt aatggtagta tgttttatat
      181 gcataaggta gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta
      241 attttaaaac taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt
      301 tatttaaagt agttcttaat gactttaaag gtagtacata attcatttta tttctgattt
      361 taggtagtat ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct
      421 attttcgaaa agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac
      481 aatgactgat tttaaatttt ttaaggcgga tagagtttac aacgaattat tttatcaatt
      541 tccaaaagtc tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc
      601 ctatatgctt ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga
      661 aggtaatgtt tattttactt atacgacaaa tgaactatgt agagtattaa actgccaaaa
      721 acaaaaagcg atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca
      781 gatgggattt aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt
      841 aaaagtctca gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa
      901 cgttgataaa tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga
      961 atcccttggg gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga
     1021 gggtatgaaa atcataccag aacttaataa taatatatta gacactaata gacacaatat
     1081 agacactgaa aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat
     1141 gacaaacgac agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt
     1201 ttcttcaagt tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa
     1261 agctgaaata gaaagtgata tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa
     1321 tgcagaacaa gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa
     1381 agtcgaaaat atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc
     1441 aatagctgct aaagtatcaa gtgaaaaacg tttgaattat gcaagctccc caagcactat
     1501 tacgaaagac tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa
     1561 cattaaaatt aaaaattttt tattattcta attatattac gaatattaca aaataatagc
     1621 tattttaata gaatttataa gttaaaatat tatgataaaa atcacaaacg tagcctttta
     1681 cgtcataagt gttaaaagaa ataattaatt tcaaatatag aaaagggaac gtatgtttgc
     1741 atactccttt tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca
     1801 agctaaaaat cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt
     1861 ttgtataaat ttaattgtct gctatgcttg tgatgaattg attttcgttt tgcacttttg
     1921 gatgataaat ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg
     1981 ctggtaacct tcgtcagcgt tcatacacgg cattaagatt tggcccttaa tgcgttaacc
     2041 tttcagtgtt acggtattac ccgcaatgga tttagtataa cagatattca aaagtgatac
     2101 cacttttttt ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa
     2161 gcataatgtt ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga
     2221 gggaacgcat gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct
     2281 ctatttttga aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat tgggtggttt
     2341 attttatgtc atatacagga attttgagtc tcaaagatat ttgtcactac ggtaaacggt
     2401 gtaccgctac agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc
     2461 agtgtaagaa atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacattg
     2521 ggaaacaaaa gcaaataatt ttaaaagatc ctatcccctt aaaagagcta tatccaacaa
     2581 tcaaacatgt ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc
     2641 gttgtaccgc aaaagggatg gggcgtttga tcggctgaat tgatcattct cataacttgt
     2701 attttatcgt ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga acttagagtc
     2761 actagaaaaa taaggagtag acttgatcta tcaacaattc ccttcgggga gtgacggggt
     2821 aggttttaac gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc ttgtttggat
     2881 agtggtgaaa tgaatttgtg taaataataa gcgcagagta aacaagaggg aatttagaag
     2941 ttagacaagt tagaatagcg tctagattaa tcatgagcag ctactaaggg atctagactt
     3001 tgacgcaaac tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa
     3061 ttgtgtcgtg tgctgcagta catgtagata cttgcgagga cgttgattat tggatcaatc
     3121 gagcatttac cgctgtctac tagggtttct cgtttcggac gccataaacg agtcgaaatt
     3181 ttttatgtat ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct gaataaagtg
     3241 aaaaaattaa tataaagcat agagaaaatg aaatttacgt aatggaggaa tgacaaatga
     3301 aaaattatga tccgaatata cgattgggca cacatactat taaggtttct ttccaacgtt
     3361 gggattacaa aggatttgtg acgtttagaa ggggcggaaa ttgtaaagga ttagatgttt
     3421 tagcattaga cgaggatgat ttatacgatc aaacattgac tgataatcca attggattcg
     3481 gcttgctacc tgaagatgat gaaggtaatg aatggtttaa aatgacgctt atgaatgata
     3541 atggagacga attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagct
     3601 ttgaaatcat tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac
     3661 agataaaggc ttagaaggag tcacgacgat taataaaaag gtagtattac cttaggcatt
     3721 gagtaaggaa ttt
//
LOCUS       DAZKLA010000185         3602 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000185 DAZKLA010000000
VERSION     DAZKLA010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.189"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..186
                     /locus_tag="ACG85N_002741"
     CDS             <1..186
                     /locus_tag="ACG85N_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0225446.1"
                     /translation="VEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTV
                     YEGHFVIAFKSGIEMEI"
     gene            329..553
                     /locus_tag="ACG85N_002742"
     CDS             329..553
                     /locus_tag="ACG85N_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0225447.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            568..1437
                     /locus_tag="ACG85N_002743"
     CDS             568..1437
                     /locus_tag="ACG85N_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0225448.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1418..2152
                     /locus_tag="ACG85N_002744"
     CDS             1418..2152
                     /locus_tag="ACG85N_002744"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0225449.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2185..3093
                     /locus_tag="ACG85N_002745"
     CDS             2185..3093
                     /locus_tag="ACG85N_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0225450.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3090..3570
                     /locus_tag="ACG85N_002746"
                     /pseudo
     CDS             3090..3570
                     /locus_tag="ACG85N_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1251 a          521 c          789 g         1041 t
ORIGIN      
        1 gtagaagatg cctccctcag tggcgagaac gagcgaatca atgagctgat tgaatttatc
       61 cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa gataatccag
      121 agcgttacag tctatgaagg ccacttcgtc atagccttca aatctggcat cgaaatggaa
      181 atatgaaaac cagaagtaaa tagcccatga ctctacagta gagttgtggg ttttcttttg
      241 cttgctgatt ataataatat tttaataact attgtgttta tcaaccaaat ggtttataat
      301 attcttagcg taaacttgga ggtgctgtat gactacggca gaaatgatta aagaactgtg
      361 tgagcaaatg aatataagtg tttccgaact tgctagacgt attggccaga ctccacagaa
      421 tttcaataaa aaattacaac gagaaacagt aaccttggat gagttgaagg ccattgctga
      481 tgtgctaggt gtcaagtttg tgcaggcatt tattttacca gatggagaag aaataaaaat
      541 atctaacgaa taaaggagga tgtttatatg gtaggtaatg ttattcaaat agtaacagag
      601 aaattatcat ctttgccttt tatagaaggc atcgtattag ggggctcacg tgcaagaggc
      661 acccatacag aaaattctga tatagatatc ggaatctatt acaattcaga ctcatttgac
      721 ctgacagcga ttaatcaaat tgctacagaa ttggatgata aaaatagaaa caaccttgtt
      781 gtgcctcccg gagcgtgggg tgattggatt aatggcggcg gatggttatt tataaatggg
      841 tatcatgttg acttgatttt acgtgatata aaacgggtag aacaaataat caaagatacg
      901 gagcaaggaa ttgttactgc caattatcag accgggcatc cccatggtta tataagtgca
      961 atgtatcgtg gagaattagc gattagtaaa atactatatg ctaagaacga aagcttatgc
     1021 gaattaaaaa agcaggcaga aatttaccct actgctctaa agaaaagctt gatgaacttt
     1081 tttatatttg aagctgaatt ctctttaatg tttgtaaaag caaatgcggg ggtagaggat
     1141 aaatattata ttgcaggcca tgtttttcgt attatttcat gcttaaatca agtactattt
     1201 gcatgtaata atgcttattg cattaacgaa aagaaagcta taaaactgct tgaaactttt
     1261 gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt actcggtctt
     1321 tctctttttg aatgctacga tatgaccgag aaactttata aagaagtgaa tgaaattgta
     1381 tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga
     1441 taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc
     1501 gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga
     1561 tttaggatgc ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt
     1621 agtaggtgtt gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc
     1681 acagattgaa tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga
     1741 tgtaatacta agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa
     1801 gatatatagg atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt
     1861 tactgctcat ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc
     1921 ggtggacaat tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac
     1981 aaaatatcat agaacactga ccacatatct aaatacactg ctttcaaata gttttataat
     2041 aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga
     2101 tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag
     2161 aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt
     2221 tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat
     2281 attaatatac ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata
     2341 gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat aatgatgcaa
     2401 aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta tcttatgcta
     2461 tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt agataattac
     2521 ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat taaaagggac
     2581 atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga gtatgatgat
     2641 tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg ccgtaaggaa
     2701 attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct gagaatgata
     2761 tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa ctataagttt
     2821 attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta ccggatggat
     2881 tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag ggcggtatcc
     2941 ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa tataacaaaa
     3001 tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga tagcacatat
     3061 gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca gggcacctga
     3121 aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata ccgaggtatg
     3181 aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta aaaagctacc
     3241 aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt gacaatactg
     3301 ataagataat atatctttta tatagaagat atcgccgtat gtaaggattt cagggggcaa
     3361 ggcataggca gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat
     3421 ggactaatgc ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt
     3481 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa
     3541 aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc
     3601 tt
//
LOCUS       DAZKLA010000186         3577 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000186 DAZKLA010000000
VERSION     DAZKLA010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.190"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACG85N_002747"
     CDS             complement(<1..2232)
                     /locus_tag="ACG85N_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0225451.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACG85N_002748"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACG85N_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         1026 a          783 c          552 g         1216 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac aaactaacta tccatcaata acgtgtttta ttttttaaaa tttctataaa
     3541 aaaacaatca ttcttaaaaa gaatatataa aaataac
//
LOCUS       DAZKLA010000187         3569 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000187 DAZKLA010000000
VERSION     DAZKLA010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.191"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(186..863)
                     /gene="nth"
                     /locus_tag="ACG85N_002749"
     CDS             complement(186..863)
                     /gene="nth"
                     /locus_tag="ACG85N_002749"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFQ0225452.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(870..1568)
                     /locus_tag="ACG85N_002750"
     CDS             complement(870..1568)
                     /locus_tag="ACG85N_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0225453.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1836..3020
                     /locus_tag="ACG85N_002751"
     CDS             1836..3020
                     /locus_tag="ACG85N_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFQ0225454.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3026..3469)
                     /locus_tag="ACG85N_002752"
     CDS             complement(3026..3469)
                     /locus_tag="ACG85N_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0225455.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          922 a          718 c          598 g         1331 t
ORIGIN      
        1 tacatcgggt attttcccaa taaaaataac aacttttatt tttatcgcaa taaattgtaa
       61 taagttacca tcctagctac tgagtatgta catttacttt ttcatgcaaa aaagataaac
      121 gaaacagctt ttctccataa taacaagata attcatacat ttatttgcaa ttaaagaaac
      181 cacgtctaca ggcgtggttt cttttttagc gccttttctt ttagacgcat ccgatttttc
      241 ccatcttgac atattgatag caaaggacat acctcgcact tgggatttct tgccgtgcaa
      301 tgatatctac caaaaaaaat cagtgtatga tgtgtcttta cccataattc ttgtggtact
      361 ttccgcatca gtgtttcttc tacttccatg accgtagcat ctagtttgca gatcctcagg
      421 cgttttgaga cacgttccac gtgggtatct actgcgattg ctggtatccc gaatgcatcc
      481 cccaatacga catttgctgt ctttctgcct acaccaggaa gagacatcaa ttcttctctt
      541 gacgttggca cttgcccatc gaaacgttca atcagctgct gtgcacacgc tttgatgttt
      601 tttgctttat ttcgatacaa accaatcgtt tttattttaa gaatgatttc atcgattgaa
      661 gcttctgcta acgcatcggg tgtcggaaaa gaggcaaaca aatctggcgt tgctttattc
      721 actgatacat ctgtcgcctg ggcacttaag atcactgcaa tcaataactc aaatggatta
      781 ttgtgcttga gttctccatg tgcttcggga aacattccat acatcgtttc tagagcttcc
      841 attgtttttt gtttgcttaa catattccgc tactcctcca accaattttt cagtgagact
      901 ttcggcaatt cttcttgttg agctgactgt tctacttctc tttgaagaag ctgctgtttt
      961 cttcgttttt ggtcttcttc cacttgttgt tttgtacgta gattttttct ttcccacgat
     1021 agcaaaatgc ggtcaacgta attaaaacta taagcctgat ttaatacggc ttcccgcagt
     1081 gctagcttca aaatttccgg ctgataatgg tcttcttcta gccattgtcc gatccgctgg
     1141 aactcgatcg aagaaagcgg gcgtccaaat tcttgttcaa ataattgata cactgactgg
     1201 atttcttttt cttgtgtttt ttcttcgtat tttttttctt gtgtttttaa atactcacct
     1261 aatagatcat agatggggta aagattatat cgatctgctt ttttaccgtt gacaatcgtc
     1321 gtttctatct tgataaatcc atttgttacg agacgatcca gtatttgaaa aatctgatct
     1381 tgtttgattc ccatatccat agcaatcatc tgcaaatcag ggaaagtatc tccttcaagc
     1441 tgtgccatat gcaactgtag aacaaataaa aattcttcag attgtagacc gatcctatga
     1501 taatttttaa gtaaaagatt agatatgact gtttgtccag cgcttaagta attttctaaa
     1561 ttcaacatga tttctcctcc gctcaatcac agtatacgta aaaaaaacat gggaagcaag
     1621 aacaggtttc ttttctattt acttcactga tcggtcagat ttacaatgtc gtctacttga
     1681 tttttgtttg ttcatgtttt tgatgcgtat cttcactttt cggaataaat aaattaagta
     1741 gatttcgctg ttccttagaa tgttgacaag gaaacaaaat tactatacac tatttttgtt
     1801 tccaagttaa cattattgca taaaggagtt tttacatgta cagtttcaca aataaacatt
     1861 ctaaagtttt tggatacgtc tctgtcttat tcactggatt aggatttact atcgtcagtc
     1921 ctgtacttcc gtttttaacg ctgccttatt ctcactccat acatcagcaa gctttctata
     1981 tcactttgct catgtctgtt tatgctctag ctgccttctt atctgccccc attctcggta
     2041 gcttaagtga tcattttggg cgtcatccga tccttattat cagtttatta ggatcaagta
     2101 tcggatattt gctttttggt ttaggaaatt cgatatggat gttgttcctt ggtcgtatca
     2161 ttgaaggatt gacggcagga gaaatctcca ccttatatgc ttactttgcc gacatcacgg
     2221 aacctaatga aagaaccaaa gtttttggat ggatgggtgc tttagtcggt ataggaacaa
     2281 cgctgggtcc tattataggg ggtctattag ctgagttggg gaattctgtt cctatattta
     2341 ttggagcact ctttactttc ttaaatgccg tttatggcta cacatttatg ctagaaagcc
     2401 ttccaataaa aaaacgttca gtagatttaa gtttctccca tgtaagacct tttcaccaat
     2461 tacaacaatt attcaagatt tcatccgtta ttcctttatt gacagcaggt tttgctgttt
     2521 ggttggcagc tggctctctt caatctatct tttctcaatt ttcgattgac gcctttcagt
     2581 ggaaggctgg gttggttggt ttaagcttct ctttgattgg aatcttggat actgtctcgc
     2641 aattgttcat tatgcctcga ttgctcaaaa aattttcaga acagcagatc acaagaatag
     2701 ggatgttcag tgaaatcctc gcctatctat tcatcacatt atctggaatt cttctactgc
     2761 cgattttgtt tttatttggg attatctgtt atggtttcgg tgattctatt tttactcctg
     2821 tattcaatgg ccaactatcc aattctgtct cagataacca gcaaggtctt gtaatgggcg
     2881 gtacccaaag tatacaatct ctatcgcgcg tcatcggccc gctaatcgca ggacagcttt
     2941 atgctgcagc accatttctt ccgacggtat tcggattttt gcttatgatt tttgcctatt
     3001 atcactacaa taaaaaatag tagcttcatt tgctacttcg tataaattct ctgtatcgac
     3061 cgataaaacg aaatactgtg tcaaattctt cttttgacat attttcaaat acagtagcat
     3121 ctcgctgtac ccatttttga tggagttgct cgtgtctatt ccttattttt tgtccgattg
     3181 gcgtcaattg aaaatagatt tcttttttat tctcttcgct ttgataagtg tttatgatcc
     3241 ctctagctat cagttttttt gttagcttac tgatggctcc tctagtcatg taactagcat
     3301 tcgctaattt tgtcacgtta ggatcaggga ttttcccgat atgttcaatc acttctagtt
     3361 catttagagt aaatcctgca aattcttttt ccatttctgg tttattacga tgaataatct
     3421 cattagctaa atccagatat tcttctatta gcagttgtct tttatccatt tctacacctt
     3481 ctttttcttt cattatattg tttttttgtt tcctattcaa caaagataaa aatattttgt
     3541 aggaaaaccc aatctgaaac caagaaaaa
//
LOCUS       DAZKLA010000188         3230 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000188 DAZKLA010000000
VERSION     DAZKLA010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.193"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..546
                     /locus_tag="ACG85N_002753"
     CDS             <1..546
                     /locus_tag="ACG85N_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFQ0225456.1"
                     /translation="KYVLQVFHPVVTELENIEKQILALINSMEQQSQPIICILPNSDA
                     GSEIIRKMYQKYLKTDIIYITNLEPEEYLTILKECKFMIGNSSSGIIEAASFKIPVIN
                     LGTRQNGREQSKNIINANLNENEIETALNKIQDPQFIKSLKNVKNIYGDGNSSRRITK
                     TLNQILNDGEDIKWIQKQIAY"
     gene            519..1196
                     /locus_tag="ACG85N_002754"
     CDS             519..1196
                     /locus_tag="ACG85N_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFQ0225457.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1147..2328
                     /locus_tag="ACG85N_002755"
     CDS             1147..2328
                     /locus_tag="ACG85N_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFQ0225458.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            2341..>3230
                     /locus_tag="ACG85N_002756"
     CDS             2341..>3230
                     /locus_tag="ACG85N_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HFQ0225459.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFT"
BASE COUNT         1202 a          414 c          615 g          999 t
ORIGIN      
        1 aaatacgttt tgcaagtatt tcatccagtt gtaaccgaac tagaaaatat agaaaaacaa
       61 attttagctt tgatcaattc aatggaacaa caatctcaac caattatttg cattttacct
      121 aatagtgatg ctggtagcga aattatcaga aaaatgtacc agaaatattt aaaaacggat
      181 attatttata ttacgaattt agagccggag gaatacctga ctatattaaa agagtgcaaa
      241 tttatgatcg gaaattcttc ttctgggata attgaagctg catcattcaa aataccagta
      301 ataaatttag gaaccagaca aaatggacga gaacaatcta aaaatattat aaatgcaaat
      361 ctaaatgaaa atgaaattga aactgctttg aataaaatac aagatcctca gtttataaaa
      421 agcttgaaaa atgttaagaa catctacggt gacggcaaca gttctagaag aataacaaaa
      481 acattgaatc aaatattgaa tgatggggaa gatattaagt ggattcaaaa acaaatagct
      541 tattaaaata tactagtgat ccatacatta ttattggggc tggcggacac gcaaaagtca
      601 ttattgaaac cttaagatta gaaaataagt taatatatgg attttttgat gataagcaaa
      661 ataaaatgca agatctgaaa tatcttggga aaataaacga attgaatagc tgggcggata
      721 gcaaaaaaaa aatctataaa tttattgtag ctattggtga ctgtgtagta agaaaaaaga
      781 ttataaaaaa attgaatatg ccaagtgagc aatatggaat agctgttcat ccaagtgcta
      841 tagtggcaaa agaaatttca attggttatg gaactgttat ttttgcgaat gctgtaatta
      901 acccagatgc tgtaattgga gagcatgcta taataaacac aggtagcatt gttgagcatg
      961 attgtagaat taataactat gttcatttat ctccaggtgt atgcttagcg ggtggagtac
     1021 atgtaggtgt aggaactcaa ataggaattg gttctcaatg tatccagatg aagaaaattg
     1081 gatcttggtg tatgataggc gcagggagca ctattgttaa ggatattccc tcacattcct
     1141 tagcttatgg caatccagct aaaattaaga aagaaggaat taattttgaa ttctagaatt
     1201 ttattatcaa gtcctcatat gggaggaaca gaagaaaaat atgttgctaa ggcttttaaa
     1261 gataattgga tagcgccatt aggtgaaaat gtggataaat ttgaagaaag tattacaaaa
     1321 tatactggtt ctaagggcgg agcttctgcg ttaagttcag gtacagctgc catacatctt
     1381 gccttaagac tgcttaatgt tggaaaaaac gatattgtct ttgctccttc atttactttt
     1441 atagctacag taaatcctat tttatatcaa ggggctgtac cagtgttcat tgactcagaa
     1501 cctaatacat ggaatatgag ccctgttgca ctagagagcg cattagaaga agctgaaaga
     1561 aacaaccaat tacctaaggc aatcatcgta gtgaatttat atggtcaaat ggctgattat
     1621 gaacgaattt tagctattgc aaaccaattt aatgttccag tgatagaaga tgcagcggaa
     1681 tcgttaggtt ctacttataa gggaatttcg tcaggcttat ttgggacaat tgggatctat
     1741 tcatttaacg gtaataagat catcacaacc tcaggtggag gagctctagt gtctagtgat
     1801 aaaaaatatg ttgagcattc aagattttta gcaacacaag caagagatca agcaccgtat
     1861 tatcaacata gcgaaatagg ttataattac cgtctaagca atatttctgc cggtatcgga
     1921 cgtggacaaa tggaagtatt gaatgataga gtagctaaac gaagagaaat tttcaatcga
     1981 tataaggaag ctttttctgg aattgataga attgaaatga tgccagaagt ggatggcatg
     2041 gtgccaaatc gttggttgtc tactttaacg atcaatgatt ataattctat tgatcaagta
     2101 gttaacgtaa taaataaact aaatgaaaaa aacattgaaa caagagcttt gtggaaacca
     2161 ctacatttgc aaccagtatt tcaaggggta aaaatgtatt accatgataa taatcttagt
     2221 gtgtcagaaa agttatttaa tagcggctta tgcttacctt ctggaagtaa tatgacagat
     2281 agtgaacaag aatttgttat tgatgagttt aaaaaaatat tcaagtaggt gtgatatttt
     2341 atgaacatag gaattattgg tttaggttcc atggggaaaa gaagacttag actcattcaa
     2401 gaacacttta atgatatttt actatgtggt gttgatacta atgaggcgag acgaaaggaa
     2461 gttgaaaagg aatttggaat accaacttat aatagtctaa cagaggcaaa aaatttgttt
     2521 gaaataaatt cagtttttgt ttgtacatcc cctataaccc atgaatttat tataaaagag
     2581 gctttaaatt tagattgtaa tatctttacg gaaatcaatt tattaaatga atattatgat
     2641 gaaataatcg aagtagctaa aaagaaagat agattacttt atctatcttc tacatttttg
     2701 aagagaaaag aaattcaata tattaagtct aaagtgacgc ataataaaaa aatttcttat
     2761 caataccatg taggacagta tttacccgac tggcatcctt gggaagatta tcgcaatttt
     2821 tttgtttcaa acaaaaaaac taatgggtgt agagaaatat tcgctattga attaccttgg
     2881 attatagata catttgggaa agttaaatcg tttactgtaa tgacccagaa attatccaat
     2941 cttgatattg attattcgga cagtttcact cttattattg agcatgaaac tggtatagtt
     3001 ggaactttaa gtgtagatgt tgtttcaaga gtagctaaaa gaaatttaac tataattggt
     3061 gaaaagattt taatagaatg ggaaggtaac ccggattctc tattttcatg gaatgaagaa
     3121 acttctagta tgacagatat tcaattgtac gatgaggtta tgcatgataa taattattca
     3181 catactatta ttgaagatgc atatttagag gagattaaag aatttttcac
//
LOCUS       DAZKLA010000189         3207 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000189 DAZKLA010000000
VERSION     DAZKLA010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.194"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..643
                     /locus_tag="ACG85N_002757"
     CDS             <1..643
                     /locus_tag="ACG85N_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0225460.1"
                     /translation="KNTMINTWNNIKDSFWNIVTGIVNSAENAWTNLKNGVSNAINRV
                     KEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAGNIKNWIKGALDIHSP
                     SRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPVVNGISHLPITQTKQN
                     ISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDAGAVGGTLYAT"
     gene            633..1373
                     /locus_tag="ACG85N_002758"
     CDS             633..1373
                     /locus_tag="ACG85N_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0225461.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1370..>3207
                     /locus_tag="ACG85N_002759"
     CDS             1370..>3207
                     /locus_tag="ACG85N_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0225462.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         1203 a          390 c          623 g          991 t
ORIGIN      
        1 aaaaaatacc atgattaata catggaataa cattaaggac tctttctgga atattgttac
       61 tggaattgtt aattctgctg aaaatgcatg gacaaatcta aaaaatggtg tttcaaatgc
      121 tattaatcgg gtaaaagaaa tctttaattc gttaagggaa gtcaatttat ttgaaattgg
      181 caagaacatc attgatggac ttataaatgg tgtaaaagaa aagtggaatg ctttgaaaga
      241 tactatcaaa ggtattgcag gtaatatcaa aaattggatt aaaggagctt tagatattca
      301 ttctccatca agatggatgc gtgatatggt tggtaaaaat attgttcaag gtattatcgt
      361 tggtattgat aaagagcaaa gtaagttaga tcaaaccatg acaaatttag taaaaacacc
      421 gtctgttcaa cctgtagtaa atggaataag tcacctacca attacacaaa caaagcaaaa
      481 tatatctgct agtgaaacaa aagaaattca tttacattta aatgtttatg gagatttacc
      541 tgattcaatg gttcggaaga ttaccacaaa aattaaaaca gaattgacaa gacaaatgaa
      601 acgagatgct ggtgcagtag gaggaacatt atatgcaact taagagagga caatttttta
      661 ttaatcaaca ttattcttct gaatttaatg tgtatattca aaacaggcct gcttctgttt
      721 cagctagtcg agtaattgaa ttgagagagc gagaaggaaa tgattctatc attattgata
      781 aaggctatta caaaaatgtg actagaaaga ttgagtgtta ctataaagca ccttcaattg
      841 atatggtgca ggagtgggaa gatcgaatca ctgaatggtt agatatgtcg tcttatagtg
      901 attttattct ttactatgat gaacaatata tttatcaagc tgtagtaata gaaggacctg
      961 aatttaaagg aacaagaaaa acaggaaata ttgtcccttt tgaatttaca gtgagtattc
     1021 gaccatttaa ggaaaattac aatggtagat ttactattcg acaaacagaa acttttgaaa
     1081 tttataaccc agagaagtat tcttcaaaac cgcttattaa attgagtggt tctggagatg
     1141 cttcttttta tattaataag gataaatatg atttgaaatc gttggacaga gaattattta
     1201 tagattctaa attagaagag gcttatcgaa aattagatgg taatttagaa catcaggatc
     1261 aggtcacctt atttttagat tttccatttt tatatcctgg aaaaaatgaa attaaatgga
     1321 cgaaaaatat tcattcattt gaaataatgc ctaggtggtg gagaaaagta tgaaaccaag
     1381 aatatatagt cctactgaaa cagatttctc aacgaatggc ttaggaattt taaaggattg
     1441 tacaagatgt ggaatatatg aagtagctaa tggaaaatat gaattagagt tggattatcc
     1501 tttaggaact agatttgatg aatattttga aaatgactat caaataaaag caaagccaaa
     1561 cgatcaagaa gagtatcata tcttttttat tgatgataaa gatatagata cttttttaaa
     1621 tacagtaact atttatgctc agagtcgtac aaatcgactt ggaagacggg tagtcactct
     1681 tgtggaagtg gactctaaaa ctggtcaaga agcgatatca attattgaaa ctaaaatgga
     1741 taaaaaatct gacatacgac tttattctga tattacggct gtttctagca caatctttga
     1801 agcgagaaat gttttaaatt gtattgctgg tgaacaagga tcattgcttc aatattgggg
     1861 cggagaaatt aaacgtgaac catttaagct ttctttgtta aagcgaagag gtcgcaataa
     1921 tgttggaacg attcggtatg gaaaagatat gtctggtttg aaggtcaaat tagattggac
     1981 aggtataaaa acaagaatta ttccatatgc tgatcctcaa agtgaagtag gtacgactaa
     2041 tcgaatttat ggttcgccag tagatagtgc ctatattaat aactatcctg atgtctatac
     2101 agaacatgtt cagtttacag aagaacaagg agtaaaagac attaagagct taaataaaat
     2161 agctaaaaat tactttaaaa cgatcaatcc tggctgtgat aagcctaaag tttctattac
     2221 ggttgaattt gataagttga ctgatagtga agaagcgaaa gaatttgcga agattagaaa
     2281 ttatggtctg tttgatacat ttaaaatata tcataaaaaa tataatattt atcttgaatc
     2341 aaaggttagt ggactacaat acgattcttt atctgaaaaa actttgaaat tagaagcagg
     2401 agacattcaa gttgcttttt atcagcaaca agctgtaact attcaggata aattaaaaga
     2461 ttatgcaacg aataattata tgagtgattt taatgattat gtttcttcaa tgattacagg
     2521 tcaaggcagt gcaggaggat atgtagtttt atggccaaaa gaaaaacctt ccaacatctt
     2581 tattatggat aatccagatt tagaaaaggc aagagaagta ctaagaatga ataaaaatgg
     2641 gattgctttt tctaagaaag gatggcgggg gccttttaat tcagcttgga cattagatag
     2701 tatatttaat gccaatttta tacaaactgg attaatcaaa gcagatatct ttcaaaattc
     2761 ttttaacaag actggagatg tattgaaatt agttaatgga ctactccaaa tttggaacaa
     2821 caaaaagaaa ataatggagt taactaaaaa aggaatggaa ttttggaatt ctaagggttc
     2881 aattggaacg attggaacaa ctgattctgt tggtaatcct tttcctgggg cttctactcc
     2941 aacccctatt gaagataatt ctttagttat tcgtacaaat ggagacggca aatatatttt
     3001 gatttctcct aaagtaggaa aaggattagt tttattagga aatggtaaag caatttattt
     3061 tggagactta gatgtacaag gaaaacttac agttaaagga aaagaaataa ttcctggaca
     3121 aggtggtggt ccatcgggag gtggagaatc tactggtggg tatcctagtg aattaaaaac
     3181 agatgcagaa aagagagctt ggagaat
//
LOCUS       DAZKLA010000190         3200 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000190 DAZKLA010000000
VERSION     DAZKLA010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.195"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACG85N_002760"
     CDS             301..585
                     /locus_tag="ACG85N_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225463.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACG85N_002761"
     CDS             588..1004
                     /locus_tag="ACG85N_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225464.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACG85N_002762"
     CDS             complement(1141..1860)
                     /locus_tag="ACG85N_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0225465.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2522..2608
                     /locus_tag="ACG85N_002763"
     CDS             2522..2608
                     /locus_tag="ACG85N_002763"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0225466.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2911..>3200)
                     /locus_tag="ACG85N_002764"
     CDS             complement(2911..>3200)
                     /locus_tag="ACG85N_002764"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225467.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1039 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt attttcattg gtccaagcta aaaaactctt
     2881 agaagcgatt ttagagccat ttaaacccac ttaattatat tctccatgaa attcgctcca
     2941 tgcgtcaaaa gctgcgtctg aaattccgcc gtcttcgttc actagttgca ttaatttttc
     3001 caacgatcgt tttctttcgg gtgtcatatt tttatattcc aacaattttt cgtctgttaa
     3061 atcgaacttg attgcttgaa ttgcttgctc taatttttct aagttttgaa aatattcgta
     3121 aggaaatttt tccatttttt ctttctccag tctcacaatt tttctagaaa taaaatcact
     3181 tctttgccgt ctatactctc
//
LOCUS       DAZKLA010000191         3188 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000191 DAZKLA010000000
VERSION     DAZKLA010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.196"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..165
                     /locus_tag="ACG85N_002765"
     CDS             <1..165
                     /locus_tag="ACG85N_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0225468.1"
                     /translation="ELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFIL
                     PDGNEIKIGN"
     gene            181..1023
                     /locus_tag="ACG85N_002766"
     CDS             181..1023
                     /locus_tag="ACG85N_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225469.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1086..2888
                     /locus_tag="ACG85N_002767"
     CDS             1086..2888
                     /locus_tag="ACG85N_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFQ0225470.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(2947..>3188)
                     /locus_tag="ACG85N_002768"
     CDS             complement(2947..>3188)
                     /locus_tag="ACG85N_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0225471.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1143 a          442 c          646 g          957 t
ORIGIN      
        1 gaacttgcta gacgtattgg acagactcca cagaatttca ataagaaatt acaacgagaa
       61 acggtaacct tagatgagtt gaaagcgatt gctgatgtgc taggtgtcaa gtttgtgcag
      121 gcttttatat tgcctgatgg caatgaaata aagataggta attaggaggt gtaatttgta
      181 ttgaaaatca taacaataaa tgatgttgag tatgctgtat ttgctgctaa tgaaggtact
      241 tccaaaccac aacctcatat catagaaacg aaatctggta ctattccaga aggaaaacaa
      301 ctgagtttat taaaagaata tcttaagcag aatgatattt ccccaataaa aggagcaaca
      361 acatattggt gcattgacaa ggttcttaag cttgattcaa gtaaagaaaa aactataagt
      421 gaaacaatcc ataaacaaaa atacttatct ttaactgaag aaaatattga gaaacagcat
      481 aagtttgtag gtgcaagctc taattatggg aaagaaggtc taataataca tgatgttttg
      541 aatgcatttc ctcttcataa tgatttaaat actattgcga tgaagattgc ggtaattgat
      601 gtaacaaatt caacacatct ttctcaatat aagagcaggt tgtctctata tgatttggct
      661 aaagtaattc tggagattcc aaattttgat gatcggttgg caaaaggaga tcctcagttg
      721 ataaatatta tcgcaaggaa cattggtgca gttaatatgt tctcatttgc atccaagtac
      781 tgtacatatc ataatgtgga agtgtgtggg agagatgatt actccatctt tgatggtatc
      841 gttaaaaata ctcttccaca ctatatccaa ggactaacta cgaataagat tgatacgtgg
      901 cgaagaagtt ttgattacga ggcatttaac gaatgtgttg gaaaactact tgatgaaaat
      961 aatatacata ttccttttag aagaagaaag ttagatcact tcttgtggta tgcgaatagg
     1021 tagaatataa gaaaaaggca tcgtaaaagt gattctggat aaaattaatg ggggaggaaa
     1081 taatagtggg attatttgat tttctaaaga aaaaacaagg aaaggttagt aaacacgttg
     1141 attatcaact ggcttctagt ctatttcgta ctggtaaagt ttttgctgaa aaattggaag
     1201 tggacatatc aaatgtagat gagtttcgta aaacttttat cgcttttgat gtggaaacaa
     1261 ctggcctaaa ttcttcttca gatagaattg tagagattgg tgcggtgcta tttaaagaag
     1321 gagttccaat tcaatcattc ggaactcttg tcaatccaaa agttagaatt gcagcctcag
     1381 cttcggcaat aaataatata actaatgaaa tgctaaactc tgctccaaat gagcaggaag
     1441 tttaccccca gttggtagaa tttttaggag atgcactaga tggaaaaatc actatgtgtg
     1501 cacacaatgc aagtttcgat tttggatttt taagtaacac attttctaga cttgggtatg
     1561 atgctaaaat taagtatgtt gatactctga gtctctctag aaaacatata aaaggcattg
     1621 taaatcataa gcaagagact gtagcggaat atttcggatt aataaatgac actgcccata
     1681 gagcggaatc agatgcagat atatgcggaa agattttttg gggtgtttta gatattattg
     1741 aggagtcgtt ggaagaacaa aaaaagcaaa tagaaaagat gactccagac ataaaagaac
     1801 ttgaagcttg tgcttttatt caagatatta ttgagaaaaa aggatgcgac accagttgga
     1861 tcaggtatcg aaaaaacagt aataattatg tcgatgtaac ttgtctatat acattcttaa
     1921 agtttaagtt tgctaagaag ggaaattaca tcattattag aaaagatgct acaaatggaa
     1981 ttgatttacc tatagaacct tgtactgtaa gtgaaggcgg tacaacaaat gtgcgagtct
     2041 attttcgtag cccctttgac ttaacgcctc tttcaaatta tattttcagt gcttatagcg
     2101 attgttataa ttctatgaat gaatatatga gtgttagtaa ttacactaaa aaagaagcag
     2161 agcaaagcct tagtatgctg aaatctattt caaaatcagc gatgaaagag ttattattcg
     2221 atgcaaaaaa tcgtggatat gatgagactg aagttaacct aaatattgaa aaagtgatat
     2281 ctagagaaga tgtaattatt aacgggttgc ataatcgagt ttcattaaac aagatacgaa
     2341 atatagggaa ttgggagaaa ggatttgatg caggttatcc atattgggaa cgtggagaaa
     2401 ctgctaggaa aaatggcaaa gtagatgaag ctattgcact ttttgataaa gctcgatata
     2461 acggctatga tgctcctgcg ctatatgatt cttatgctaa agcttatcga aaaattaagg
     2521 attacgataa tgaaattctc attcttgatg aaggaattat gcgtaaaaca cggcacgatg
     2581 ttggtacttt agaggctcgt agggataagg caattaagct attatttgct aaacaagagg
     2641 cagagcggat agctaaggaa aaatctgatt ttctaaaagc gaacaaaaaa gaggatgcga
     2701 ttagtgagcc tagcaagaag cgtggaagac agattattca aatgactgat gatggaacaa
     2761 taataaaaga attcgatact atagcttctg ctgtaaaaga agttggcgtt aactcaaaaa
     2821 gtattaggga tgctgcaaat ggagttcaaa aacatgctgg aggatactac tggaaataca
     2881 aagattagca gataatctaa gataacctag gatcttctta atagataccc gtggtgctag
     2941 ttaattttaa tatcctaaag aatatctcaa agttccttca aaacgtcgtt gtgctcgact
     3001 taatagaaca ctgtaagcca gtaatatgct ttcaaatcta ctctctagcc ctttaacaga
     3061 acgtgagttg aaattttgac aacctaattt ttctaaggaa gaaaaaacag tcccaactgt
     3121 ttttctttgt tttccaagta agctgtggtc tagtttttct gatttatcca tgtttttacg
     3181 tggcggaa
//
LOCUS       DAZKLA010000192         3071 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000192 DAZKLA010000000
VERSION     DAZKLA010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.198"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            287..1102
                     /locus_tag="ACG85N_002769"
     CDS             287..1102
                     /locus_tag="ACG85N_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0225472.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1228..1485
                     /locus_tag="ACG85N_002770"
     CDS             1228..1485
                     /locus_tag="ACG85N_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225473.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1737..2309)
                     /locus_tag="ACG85N_002771"
     CDS             complement(1737..2309)
                     /locus_tag="ACG85N_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0225474.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2325..2930)
                     /gene="fic"
                     /locus_tag="ACG85N_002772"
     CDS             complement(2325..2930)
                     /gene="fic"
                     /locus_tag="ACG85N_002772"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFQ0225475.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT         1018 a          539 c          473 g         1041 t
ORIGIN      
        1 aaataagaaa ttcaaaaaag aaaaaacttt ccttaaaaac aaaaaatagg agtattcagt
       61 ttcttaatag ttttgtaata gttgtatgta gaagttttct taatagtttt tgtaataatt
      121 tattaatcgt agtttatata acttatttgt aagtgactag agaattaaat aaaaaataat
      181 tactatctag tacgaaatta attacatagg ataatatatt aaggtgctgg actacaagta
      241 aaaaatactg tatgatcatg acagattcaa gaaaggagca ataagattgg ttaagatagc
      301 aggactttct aaaggtggaa aaaccgtttc acaactttat aaacaaaact acagagactg
      361 gagacagttc agaaaaaaca ttaaggattc ttactttatt ctaccaacag aacttgaaaa
      421 atatttaccg aataacaaag ctattaattt atatctgtat tactgtttta aggcaaagaa
      481 tgaaggcggg gattcctggc attcggtcaa taccatagca gagtctctag gagtgacaac
      541 taaaactata aacacctgga ataaaacctt agttgattta ggtatgattg cacgtataga
      601 cgataaccat ctcagtaaat cgacaatttt acttcctctc tctagttact ataaattagc
      661 ccttgatagt tctttaaagg atgttaccga gagcactgtc catgaaattg atggcaactt
      721 ggttgcagtt tatcatttgt ttgaatggag aaaaaatgag gctgatgaaa attataacgt
      781 tccttataac acactctgtt gtgtttttaa aagaaaaaca caaaaagata ctatctataa
      841 atttatcttg gtccaagatg agaaaataag taatttcgaa ttaagcactc cttctagtaa
      901 gatttatgac gatgcatata gatttgaatg tcaagatcta gaagccctcc tttccgcaaa
      961 taatctaaac aatatagaac atgaaaactt tgttgtaaac actagattta atttagtttc
     1021 tgaaaaggac tcagatttgt tagatttgct gatagagctt actaaaaatc tagataagaa
     1081 agaaaacatt actgtactat aactagcatc ggtccatcat taagtttgga ctattagaac
     1141 acttgctatt acagaaaaag gtatgctagc cacgcttact atttattggt atcaaacaag
     1201 tatcttaatt tgaaaggaag atgatttatg gctaatttat atatagagaa caaaggagac
     1261 ttgaatgcca ttattacttt aatcgtagtt atggtaaaat caataggtaa tatcacaata
     1321 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
     1381 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
     1441 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
     1501 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
     1561 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
     1621 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
     1681 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1741 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1801 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1861 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1921 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1981 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     2041 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     2101 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     2161 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     2221 ttctctacgc cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     2281 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     2341 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     2401 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     2461 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     2521 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     2581 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     2641 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     2701 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2761 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2821 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2881 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2941 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     3001 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     3061 gcacagtcta g
//
LOCUS       DAZKLA010000193         2966 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000193 DAZKLA010000000
VERSION     DAZKLA010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.199"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1141
                     /locus_tag="ACG85N_002773"
     CDS             14..1141
                     /locus_tag="ACG85N_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0225476.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1359..2501)
                     /locus_tag="ACG85N_002774"
     CDS             complement(1359..2501)
                     /locus_tag="ACG85N_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0225477.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2506..2691)
                     /locus_tag="ACG85N_002775"
     CDS             complement(2506..2691)
                     /locus_tag="ACG85N_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0225478.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
BASE COUNT          982 a          540 c          465 g          979 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcgatac cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata
     1321 ggctatgaca gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt
     1381 tcatttgcga gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt
     1441 ttaatagagg catgacctag acgcttagat attgcataga tatttttatt atgttcaatt
     1501 aaaaaggcaa catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt
     1561 tcagctagtt tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa
     1621 gaaaaaacca attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa
     1681 tcttcaacgg tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca
     1741 gcaaattcat aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca
     1801 tttattaagt caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta
     1861 acaaaaataa aacgcatcaa acgtttataa aattcttcat aaaaatcccc gtgataaaca
     1921 ctattatata ctttcttaaa ttcatcaact gtccaaaatt ctacttgagg acgctgagtt
     1981 tttattttac ctatagtatc tacaggattt tcttcaataa tttcaagtac aattgccctt
     2041 ttaaaaacta ctctcaactt attgaatatc tgtttaatat aatttggtga taatggttgc
     2101 tcaatgccct tttttacaac atggattttc gataaaaact gttgaaattc ctgtatatga
     2161 attggtcgaa tctttacaac ctgcatttct ccaaaaaaat ccaatgcacg ttgtaaaata
     2221 tgatctgttt tcatatatgt tttttctaca gttcccaact tataccaagg aaaaaaatac
     2281 tctcgataaa aatcatgaaa cgttattcct tgcagataaa ttccttgtga atcagaaaaa
     2341 ctttgtttta actcttcgag tccccttcta gcttctcctt gagttttgaa gcctcttctg
     2401 gtcacttgaa ttctttttcc aaagcgatct tgtcctaaag ttgcagaaaa aaaccaagtg
     2461 cctctttttc tgtctttata aacattcgca attttcctca ttttactatt cctcccaaga
     2521 aatatggaga acttcttcta aaattgaaat aggaattctt tttatttttg aattacgata
     2581 aaaaaagtat cctttctcaa ctaaaagttc tttacattcc ttgaagattt tattagcata
     2641 gtactgagaa aaacctagat taatcaaatc ttttcgagta gctgttttca ttatagcacc
     2701 ttccttactt tttttcattt atttctatca gctgcttttg tcaaaagcac ataggaattt
     2761 cacctctccc caagtttacg gcgagcctta cggaagtatc attatcccaa ctttaaatca
     2821 tcgcttttta ttgactataa aaattatttt agaatattta tcgctccttc tttacagcaa
     2881 gaaatcatgg cgcaactaaa atatgctcct taaagtgtta acgtactgat agaatctatt
     2941 taattttcaa tgaacaatag gttttc
//
LOCUS       DAZKLA010000194         2944 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000194 DAZKLA010000000
VERSION     DAZKLA010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.200"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(373..657)
                     /locus_tag="ACG85N_002776"
     CDS             complement(373..657)
                     /locus_tag="ACG85N_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0225479.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(1176..1661)
                     /gene="vanZ-A"
                     /locus_tag="ACG85N_002777"
     CDS             complement(1176..1661)
                     /gene="vanZ-A"
                     /locus_tag="ACG85N_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0225480.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(1814..2725)
                     /locus_tag="ACG85N_002778"
     CDS             complement(1814..2725)
                     /locus_tag="ACG85N_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0225481.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          893 a          548 c          426 g         1077 t
ORIGIN      
        1 gattcataca aactccacct tctataaatg aatacaaaaa aagcaatcaa acgatttccg
       61 attgattgct taacaattct taaattcagt agcttagata cttgaaaact ctctgatttc
      121 cctatataat gatagtacgg ttatataccg tcttcaaaca aagttaatta aataacttct
      181 tacgagggaa gagttcatct gactaactga taagcgttgg tttggcaatc ttatcgggct
      241 atgcatttat aaaatgtcgt caaacatttt ataaatgtgt catggctctt ttttcgtttc
      301 tattcagttc gttgtttcgt tatatctagt ataccgcttt taaaaaaaat aagcaacgat
      361 ttcgtgcatt attcacacga agtcattgct tttttcttct tccatttcta aatccaatgt
      421 tacttgttct gattctgttt ctggttctgg ttctgttggc tcatttggga ttaaatccac
      481 tactagcgtt gagttagtta actttgcaat ttgttctagt gtttttatgg ttggatctga
      541 ttttcctgat tctattcgtg aataatttga tctactcatt tctaattctt gggagaattt
      601 ttcttttgat ttggtacctc tcatcttttt taagctttct ctaaatgcta gttccaagtt
      661 tctcccccct ctttttgtgt aataaaattc acgctttatt taaattatag cttttaatat
      721 cttaataaac aagtgattaa tcacttgtga tttgtgatta ttccaaaagt aggtaatttc
      781 accaaccttt ttcaaaagtt tttatcgacg attattggac tggatcgtgt ttcctaaagc
      841 gattctaagc gcatgtttaa ttttaaagtg ggtaattctc tttaaaacgt cctacaaaga
      901 ttcaaaaaaa gcatggctgc tagtgggggt accgtcagga aaatgcggat ttacaacgtt
      961 aaggaaattt attaaagaga ccaattcctc agcactaaaa ttgataattg ggctttcttt
     1021 gttactatag tgttaggtaa gctttttact ttatcaccgg ttttcccctc acttcacacc
     1081 tacgggcgag tttccccaca tcccgttttc catctaatcc aattcattca atatctttat
     1141 gtgtatgaaa gatcagattg cttgatttag acatcttaca cgtaatttat tcttaaatgg
     1201 gtacggtaaa cgagcaataa tacgagcaaa agtataccta caaaaataat aactctgtct
     1261 aatttttttt gattcatatg cttattgctt aaaccatata atttcagtcc aagaaagcct
     1321 ccaacagtat ttgtaattac atctgttatg tctgtcgctc caatagcgaa gataaattga
     1381 attatttcaa aagtaagact taaaaccagt acaaaagcaa acttaggtaa aaatccgatt
     1441 tctttaaaat tgacattcaa aagcaagcca aatggaataa agattataac attatctatc
     1501 atctctctga aattcccagt agcagtaaat ggagtcaagt taagacttct ttgatgataa
     1561 ttaaatactg ataaaatatt gtattgtaat ttgaataaca ctaaccagat tagtgtcact
     1621 aaatataaag ctagcaatcc tctagataat atttttccca atataatttc ctctaattct
     1681 tttgtttaag ttgtatcatt agtatagcaa tgtcctagtt aacgtgggtt ttaaattaaa
     1741 aagctaacgt taccccaaaa gtgctagtac aataatgctg ttcattaatt tcgttggttt
     1801 cattccgcca tccttacctc cttgaattag tatgtgttga tccgggaaac actgtcacaa
     1861 ttacaccgtc tatattgttt cctgatatct cataacgaag attagtcggc acatgaatgg
     1921 tggtattttt agtaacagga taataaaaga tctcatattt ttccccattt acactaacag
     1981 aaatggtttt ttcttctttt aggtaatcca tatattcctc gagaacgaaa ttcttttctt
     2041 tcataatcgc actatgtggt aaaccaacat agcgaatatg ccatggttca tattgaattc
     2101 ctgttaactc tgttttgtcc tctggataac gtaaaatgaa cccgtatttc caagcatttt
     2161 cttctatcca ctttccttca ggggctcgtt ccattttcgt caagcttgat cctacatcta
     2221 gtgataaacc tgaattatgc tcactataac ctgctggtaa ggcatactca gcccccattt
     2281 cttggtaaag cacactttgc tcatcaaagt ctcgatagcc actattaata ataaaatgac
     2341 taacgccacc ctttacagca tcattgacca tctctgaaaa tttttgtgct atttcttttg
     2401 acatataaat attactatca agcaacccgt atccatttat taattcgtca tgtttagata
     2461 aattcacgat atctgacttc acactttctt ggcgaacagg atatttacta ttgattaata
     2521 gcagatttcc ttgataaacc tgttcttctg taattgtttt ctcttgggta ttttcagaag
     2581 tcccactatt ttctaaatgt tctttgggat tttgatcata attttgaaat tcgacttttt
     2641 cctgagaaaa cagtgcttca ttaacgtagt cataacctaa gtatattaag aataacaata
     2701 acaataaaaa aaacaacttc ttcattttca gtctcctcct taactatata gttggaagta
     2761 tgaaatattt ctagaatctt ccccttaaac ggcacaatct cgtttgagat tgtgccgttt
     2821 tgccccgtaa attacagtca attatttcgc cctactggat ttactattat caccaatgta
     2881 gatgaatttt cataagtgat acccatgctg aatttaacga ggctgaactg cctgttgaca
     2941 atgg
//
LOCUS       DAZKLA010000195         2889 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000195 DAZKLA010000000
VERSION     DAZKLA010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.202"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            563..>2889
                     /gene="esp"
                     /locus_tag="ACG85N_002779"
     CDS             563..>2889
                     /gene="esp"
                     /locus_tag="ACG85N_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFQ0225482.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1048 a          441 c          566 g          834 t
ORIGIN      
        1 aaagaatcaa taatttaatg aggctgtgac aaatgtcatt ttttgtttcg ctaaaaacga
       61 gtgaaaaaat gttgataact ccaatcattc gtttaagatt ctaggaatca ttcagaaatg
      121 ctgtgaaacc gatactttat aattataaag aaggtgattt caaattccga agaaggaatt
      181 tgaagtcacc tttttactta tgtcacagtc tcattcagta atgcgggata aggctcctta
      241 ttccgttatt tgaatactct gaagtttaat ctaagaaagg gtaatatttt gaaaacatgc
      301 ttcatcgcat agaaatgaac ttaatttcag aaagaataca tacatatctt tttatatgtt
      361 ttcagctgct gaatggttaa aaagcacaaa tatccgaaaa aaacatgaag aaatgtactt
      421 tttttgttgt aatgttcatg aaattctcta agtaaattag ttaacataag gttagtaaga
      481 ttcctattct gtgaataata cgtatcagat ttatctttta ttcttactag gaaagtaatt
      541 attaagggag ttgatttgaa taatggttag caagaataat aagagagtat ttctagaaaa
      601 aacaaagaaa cgagtattga agtacagtat caaaaaatta agtgttgggg ttgcatctgt
      661 tttgattggt gttggtcttg tcttgggaac aactgaacta gttaaagcac aagatgaaat
      721 ttctccaagc agcccacttg aaacagcgat aagctccgta caagtaggtg acaaagtagc
      781 ttcggggaat acttttcagg agaatcctgg ttatacgaaa aattataact tttcggattt
      841 acaatttagt cctcaagaac taactggaga cacactaaaa ggcaatacaa ttggatttga
      901 agtttacgga aaacataata ttgcagcatc aactaaaaac tgggaaatcc gtcttcaatt
      961 agatgaacga ttagctaaat atgtagaaaa aattcaggtc gatccaaaga aaggaattgg
     1021 atcttctaga cggacctttg taagaattaa tgattcgctg ggtagaccta caaatatttg
     1081 gaaggtcaat tatattcgtg caagtgatgg attatttgct ggggcagaaa caaccgatac
     1141 gcaaaccgct cctaatgggg taattacgtt tgaaaagagc ctagatgaaa tttttaaaga
     1201 gataggcata gacaatctca agaccgatcg tttaatgtat cgaatctact tggtaagtca
     1261 tcaagacgat gataaaattg taccgggtat tgatagtacg ggctactttt taacagattc
     1321 agatgatttc tataatagct tagatgtatc tgaaaataat cctgaccagt ttaaacatgg
     1381 atcagtaagt gcaaaatatg agaaacctaa tactcaaaca aaagatggtt caggatccac
     1441 aggtgctaat ggagctataa tcttagatca taagttaacg aaaaattata atttctcata
     1501 ttcatcttcc gcaaagggca cgccttggta tgctaattac aaaattgatg aacgattagt
     1561 tccatatgta gcgggtatac agatgcacat ggttcaggct gacaaagtaa catatgatgt
     1621 ttcgtttgaa tccggaacaa aagtagctga tttagcaatc gagcgacggg aaggtcatga
     1681 aaattatggc ataggttcaa taactaataa tgatttaact aaacttatcg attttgctaa
     1741 tgcaagtcca cgtccagtcg ttattcgata tgttttacaa ttgactaagc cattagatga
     1801 aatcttagaa gatatgaaag caacagcaca agttgaagaa aataaaccat ttggtgaaga
     1861 tttcatcttt gattcttggt tgtcggatac gaataaaaaa ttaatccaga acacttatgg
     1921 aacaggttat tattatttgc aagatattga tggtgatgga aaccctgacg ataaagaaga
     1981 gagcggagac acgaatccat atatcgggaa acctgaatta gaagaagtat atgatgttga
     2041 cacaacagtt aaggggaaag tattcatcca cgagttagcg ggaacaggtc acaaagccca
     2101 acttgttgat aaagaaggta ctgtattagc agaaaaaact atcgctccaa atgaaaaaga
     2161 tggggctcca atttcagata ctgtagaatt tgaatttacg ggtgtagatt caagtaaatt
     2221 aatcgcgaaa gatgaattaa aaatccaaat cgtttctcca ggttttgata aaccagaaga
     2281 aggttcaacc gttattaagg aatcaccaaa agcggttgat aaacaaaccg tggtagttgg
     2341 atttaaacca gatgctaaag aatcaattcg gaataataaa aacttacctg aagatgcaga
     2401 gtattcatgg aaaacagagc ctgatacttc taacgttact gatagtacga aaggtattgt
     2461 aactgttaag atcggaaatc gaactttcga cgtggatgta gagtttgctg taaaagcttc
     2521 tcaagctatg gaaaatgatg caacatacgt acctataaca acaactccag aaacgacagt
     2581 tcaaagtgga aaacctacat ttgataaacc agatgttcct ttagctaaag atgccttttc
     2641 aatttcagat gtctataata agtacttcgg taatgcaagt gttgatgcaa acactggtat
     2701 tgttacattc actccagcta aaggtgtagg agaatcggag ccgattactg gaacaattcc
     2761 tattaaaatt gtttaccaag atggttctgt aggcacgacc gatttagcag taactgtaag
     2821 taaagatatt tatgaaaatc caggagaaaa cattcctgca ggctaccaca aagtaacctt
     2881 caccgcagg
//
LOCUS       DAZKLA010000196         2788 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000196 DAZKLA010000000
VERSION     DAZKLA010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.203"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            234..1559
                     /locus_tag="ACG85N_002780"
     CDS             234..1559
                     /locus_tag="ACG85N_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0225483.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1552..1902
                     /locus_tag="ACG85N_002781"
     CDS             1552..1902
                     /locus_tag="ACG85N_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225484.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1884..2069
                     /locus_tag="ACG85N_002782"
     CDS             1884..2069
                     /locus_tag="ACG85N_002782"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225485.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
BASE COUNT          982 a          441 c          539 g          826 t
ORIGIN      
        1 tttttttcta atataactaa gcaaaactgg ataatacgac acttattttc aaattttcac
       61 aagactaata gtaaatatcc atttaataaa gtgatacaat gtgttgattt tacaaatcaa
      121 cagaagaagt agggaaataa cttacctatg cttctttttt tgtacattta atcgattctt
      181 ctttacaaaa acgaacgtat gtttgtataa tgtggaagaa tggagtgtta tttatgatgg
      241 attattcaaa agagccagta aatgattact ttttgatcga tatgaaaagc ttttacgcaa
      301 gtgtcgaatg tatagaaagg aatttagatc cattaacgac tgaattagtc gtcatgagtc
      361 gagcggataa tacaggatca gggttgattt tagcttcttc accagctgca aagaagaagt
      421 acggtatttc taatgtcagt cgaccacgag atctaccgca accttttcct aaaacgttac
      481 atgtcgttcc accacgcatg aatctctata ttaagcgaaa tatgcaggta aacaatattt
      541 tcagaaggta tgtagcagac gaagatttat tgatttactc gatcgatgaa tcgatcttaa
      601 aagtgaccag atcaatgaat ctttttacga ctgaaggaac aagaagtcaa cggagaaaga
      661 agctggctca aatgatccag gagaatatta aagaagagct aggattgctt gctacagtag
      721 gtgtcggaga taatccctta ttagctaaac ttgctttaga caatgaagca aaacataatg
      781 atggattcct tgctgaatgg acgtatcaaa atgttcctga taaagtatgg aatattcctg
      841 aaatgacaga cctttggggt attggatctc gaatgaaaaa aagactcaac caaattggta
      901 tcatgagtat taaagactta gctaattgga atccatacac tattaagagc catttaggtg
      961 tgattggctt acagctgtat tttcatgcga acgggatcga tcgaacagat atagcgatcc
     1021 caccagaacc gacaaaggaa aaatcatatg gtaattcaca agtgttacca cgagattata
     1081 ccaggagaaa tgaaatcgaa ctggtggtaa aagaaatggc ggaacaagta gctattcgta
     1141 tcaggcaaca taactgtaaa acaggttgtg tccatttata cattggcacg tcgatcttag
     1201 aaacgagacc aggtttttca caccagatga agatccctat aacagataat acaaaggaat
     1261 tgcagaatta ctgtcttttc ttgtttgata agtattatga aggacaagaa gtaaggcacg
     1321 ttggaataac ctatagcaag cttgtgtaca ctgattcgct tcagctggat ttatttagtg
     1381 atccacaaaa gcaaattgac gaagaaaatt tagataaaat tattgacaag attcggcaaa
     1441 aatatggttt tacgtcgatt gtacatgctt cttctatgtt agaaggcgct cgtagtatta
     1501 ctagaagtac attggttggt ggccatgcag gcggcgcagg agggcttaaa aatgattgat
     1561 tataatgatc gtggacgttt aaagtgggca ggtttttatt tatctgatca tactgaaaaa
     1621 attgatgccg acaatgaaca acgagccaat caaaatttgg caaaagaaca aatgacgact
     1681 gaagaggttg cggaagtatt gaacgctgca gtcataaaaa atagatcagt atgtattcaa
     1741 aaagaagaac gaaacgctga aggatactat ccagaagata ttgttagcaa gatcttaggt
     1801 agtgatgagc tggggatcta cgttggaaaa aacaaagttc actacgacga aatacgtcat
     1861 atcgaatttt accaggataa taaatggggt cgattagaat gaacattatc cagcaatatg
     1921 agctaaagta tataaccttt gatcagctat cagaagagat ttggggttat ggccagcgtt
     1981 tgataaatga ggttggtgtt gaacgcttct ctttctatgt agaagctgca gcaggatacc
     2041 ataaatacga atactatatt ggctattaaa gtgcaataaa attatatttc ttttatgttt
     2101 tacattttgt atcaattctg ataaaattac ctataggtaa ttaaaaaata taaaaaaact
     2161 agttacaacc ttaggacgtt gtaactagct tgaagcatac tagagctaac taaaatagca
     2221 ctagaaagca atgttaaacc cgtccaacaa acttgattta acattgagac cagcttattc
     2281 agctaaatat agttgaataa gcttcttttg ttttaaaaaa acaaaaggtg tactacgaat
     2341 aacgcaagta aaagaaatac tattacaacc agtaggctac aagtaagtat tactaaacat
     2401 tgcgtagtta gaacctttgc tctattgtct aaagccacaa aattagagat cagctttatg
     2461 atgctaagag cgagctgcat ccatgcggac ataggttctt ttgacactat taattctcct
     2521 ttttaggata agggaaattg ctagtagttt tttcattagc accatccccc ttcatttgat
     2581 tgagtctagt gctgctgtga tagtttgctg tttaatatgc ttacattctt attataacat
     2641 aaatgaaaca aacacatggt tagaggtcgt atcaatcaaa atagtgtctc acaaacgatt
     2701 tattacttac tcctgctgac taacggactg ttcttcagta actgtaacta tatgtttgtt
     2761 aaaaccacca ttacctgtag ataaaaac
//
LOCUS       DAZKLA010000197         2783 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000197 DAZKLA010000000
VERSION     DAZKLA010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.204"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..978
                     /locus_tag="ACG85N_002783"
     CDS             <1..978
                     /locus_tag="ACG85N_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0225486.1"
                     /translation="LTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIG
                     GVFWNYSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFI
                     PELFIIFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTI
                     QGLLEYASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSE
                     FYKSFGNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIA
                     IMSVFFFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            975..1694
                     /locus_tag="ACG85N_002784"
     CDS             975..1694
                     /locus_tag="ACG85N_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 32 protein"
                     /protein_id="HFQ0225487.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            1709..>2783
                     /locus_tag="ACG85N_002785"
     CDS             1709..>2783
                     /locus_tag="ACG85N_002785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0225488.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALI"
BASE COUNT          977 a          303 c          544 g          959 t
ORIGIN      
        1 ttaacaatta aagattacta taaagtattc gctataattg aaaaattatt ctggatttca
       61 ctgccacttt gtttaattga aagattcatg gtcacctatc caactggaac aattgtagga
      121 gatatgatag gtggagtttt ttggaattat tcaggttgta atatgccatt gaatttaatt
      181 ttgtgtgtga cagttactgt tgttacaatc ggatatttca agggagaaaa aaatttcatc
      241 cattttattt tatgtgtttg tgcagcactt tttatgtctg cattagctga attgaaagtt
      301 tttattcctg aattatttat aatatttgtt gcaatagtat tcactttaaa agttgatttt
      361 aaagtagtga tcaagatatt atcatgcgta atacttgtgt cgatagtatt atcctttttt
      421 ataacaatat ttattacatt gaacgataat ggacaaagct atgctgataa tttcacaatt
      481 caaggtcttt tagaatatgc gtcaagagat tctggatatg atggtgttgg tgatttaaat
      541 cgaataactg gtatctctag agtagtagat gaaatattta ataataattt tagattagtc
      601 ctaatgggag taggcattgg caatgcagag tttaccaatt tttttgtcag tgagttttat
      661 aaatcgtttg gtaatcttca ttatcagtat tttcatcata tttggttatt tatagaagtt
      721 ggaatattag gagtttcgat attcctattt tttataataa agacattttt caatgcaaaa
      781 agatctttta tgaaggataa agatgcaagt cttgttaaag ttattgcaat tatgtcggta
      841 ttcttttttg tgtataacac aacacttagg ggtgaaacaa cggggccact gatttatatg
      901 attttagcca tgcctgaagt tgtgaaaagg aaagagcatt attatagtga aaaaaataca
      961 aaaggagaga tattatgatt cctaagaaga tacattactg ttggttcggg ggaaatccac
     1021 ttccgaaaaa agatagaaag tgcatagaaa gctggaaaaa gtattgccct gattatgaga
     1081 ttattgaatg gaatgaatca aattatgatt taaataagaa ccgttatatg gccgatgcat
     1141 ataaagaacg taaatggggt tttgttccag attatgcaag atttgatatt atttataatc
     1201 atggtggatt ttatttagat acagatgttg agctaattaa gtcgttagat atacttcgtg
     1261 aaaatcatgc ctatatggga tttgaagggg aaatctgggt taatggcggt attggttttg
     1321 gtgcagaaga aggacattct gttattaaag gtttaaggga tatttacgat tcattaagtt
     1381 tttatcaaaa tgatggtaca ttgaacttga aaccgagtcc gtattatatt acagattatc
     1441 ttgaatctat tggattggtt cgcgataaca gcgaacaaat aataaatggc ctgcatatct
     1501 atcctacgga ttattttgct gccaaggatt atgatactgg aaaaatttat aaaactagta
     1561 atacaataag cattcatcaa tataatgcat cttggaaaac acctaaacaa aaatttgttt
     1621 atagattaaa atcaacaatt ggacaaaaaa aatttaacag gttacttgca ctgaaaaaat
     1681 ttttacactt ataaaggaga aaagtacttt gaagaaaatt tgtttcatta gtggtgtaat
     1741 ttctcgttct gggggaactg aaagagtagg gagtattatt gccaatagct tgtctgattt
     1801 aggttatgat gtcactattt taagcttttg gaatagtgga gagccttatt ttgggttaga
     1861 taagacgatt aaggttgatt atatgttaaa tcctaaaacg gagggaaaat tatatagaac
     1921 ttatttatat ccggtttgga aattgcacaa gtatatatgc aaaaatgaat ttgatatagt
     1981 aattgatatt gatactttgt tgtctttgta tactacaagg gcaattaaag gaacaaagtg
     2041 caaattgatt tcttgggaac attttaatta ctggacaatg gaaatgctga aagaaaagaa
     2101 gagattcaag gctaaaaaat tgataaaaaa atatgcaaag aggcttgtgg ttctcacaga
     2161 agaagatcgt caaaaacata ttgatgaata tagtttatca gaagattttg ttataactat
     2221 gccaaatccg tgtatatcta atatcgagta cgaatatcat tatgataaaa agagatttct
     2281 tgcagtaggg cgattagtgg aagagaaaaa ttttgctgac atgataagtg cttggaaaat
     2341 tgtagaaaaa aaagtagatg attgggatct tatcattgta ggaaaaggtg aattagaaaa
     2401 agatcttcgc cagcagataa aagatttaaa tctgcaaaga gtaactatag cagggcattc
     2461 agaccatgtg gaggaatatt ataaatctgc atcttgttat gtgcttagtt ctgtttatga
     2521 aggatttcct atggttatat tagaagcaca gtcttatgga ttaccagtta tttcttatga
     2581 ttgtaaaact ggtccgagag atttggtaca tcataatttt aatgggatgt tggttgaaga
     2641 taagaatata gaccaattag ctaagagcat gataatgttt actaagaata ctgatttagc
     2701 aatgagaatg tcactgaacg catataataa tgtacaaaaa tttaatttga aagaaattac
     2761 aaaacaatgg gttgctttga ttg
//
LOCUS       DAZKLA010000198         2749 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000198 DAZKLA010000000
VERSION     DAZKLA010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.205"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..438
                     /locus_tag="ACG85N_002786"
     CDS             64..438
                     /locus_tag="ACG85N_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0225489.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            443..871
                     /locus_tag="ACG85N_002787"
     CDS             443..871
                     /locus_tag="ACG85N_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0225490.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            921..1574
                     /locus_tag="ACG85N_002788"
     CDS             921..1574
                     /locus_tag="ACG85N_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225491.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            1710..2276
                     /locus_tag="ACG85N_002789"
     CDS             1710..2276
                     /locus_tag="ACG85N_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HFQ0225492.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            2392..>2749
                     /locus_tag="ACG85N_002790"
     CDS             2392..>2749
                     /locus_tag="ACG85N_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFQ0225493.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDL"
BASE COUNT         1042 a          453 c          528 g          726 t
ORIGIN      
        1 gtcaaccaaa aatataactt ttaagctatt tattttcttt tcagctttga aaggttgata
       61 ataatgagtt tagttactaa gattaaagaa ttagcagacg aaaagcatgt gactatagca
      121 gaagtagaaa gacaggtggg catctctaat ggacaaataa gaagatggga taaagcctcg
      181 ccaaaatctg agaacttaaa gaaagttgct gattattttg gtgtcacaac tgattattta
      241 ttgggaaata ataatgttcc caagtgggct acaaaagagg aagtggttga acttgataaa
      301 ctactagact caaatgttaa tatgtcttat ggtggggaaa cattgacacc cgaacaaata
      361 cagcgcgtaa aagatatcct gatagcgact ttctgggata ttgtgaaaga agacaaagaa
      421 aaaggcaaaa agatgtgagc ttatggagat ggatacgatt aatttagtcg aggagttgaa
      481 gcggaaatac cagtccgcta atccttttta tatttgtgaa aagatgggca ttaaaattca
      541 atacgttcct tttatcgaga atcccaaggg gcagtttcaa gaaattagag atcgtgcgat
      601 aatcttttta aatgatgaac tgcgagactc tgaggaaaga ttctacattt gcgctcacga
      661 attaggtcac gctatttttc atcgtggctt atccagctat tatgtatcaa caagaacatc
      721 tagaagcaaa tctgaaagcg aagctaattg ctttgctgct aatctcattg tttctcttta
      781 taaagaagat aatgatcaat atcctaaaaa aatcgaggaa ttaaagaatc tttacgggct
      841 tccagaaagc gcttatcgtt ttcttatata aaaaagcccg tgttagcaca catattacaa
      901 cgagaaagag gaattataaa atgaaaaaag ttagcgttat gttgttgtta agtactgctc
      961 tgctactttc agcttgttca aataataaaa aagctgaatc aacagatacc acttctaacc
     1021 aagaaacaaa aataagtaaa acaaaagaaa caactgaaac cagttcatct actagcaaat
     1081 ctacatctaa aacagattct agttcaacag ttacaagctc caaccaagct acggcggaac
     1141 ctagcccaac agttataagc tccagtcaga gtacaatcca aaccgcacct caagaagaaa
     1201 catatgaaca gatgaaacaa cgcactttac agtcaactcc agctgatcgt gcaaattggt
     1261 ccaacaaaga gtgggaagct ttcggcgtgg ccctttatga aaatggattg actacagatg
     1321 atgctggcaa tattatcagt caagatcaga aagaacaaca agcagcatct caacagaatc
     1381 cagaagacca acaaacaagc gctcagcaag acgctgacac tttatcactt actgattttg
     1441 ttaacaaata cgggatgtcg cctgttgcat ggaaagtaca gaatggaatg tctgaagaag
     1501 aagcattgcg tacaacacag caaaagactt ccggtgaagt tcaattagga ttttctaaat
     1561 acggaattca ataatacatt tttatgccct actattttgc ctataatctc taaaaaagtt
     1621 ataaagaaaa aagcccgtgc tgcaacacgg actcatacct catttctgag atcacaaata
     1681 tattataaca agaagcgagg gatatttaaa tggcaaaaaa agttatgggt caagatggga
     1741 aaatgtataa agttaagaaa ccgttttata aacgggtatg gttttggttg ttagcggttg
     1801 ttgtggtgtt tattgctata ggttcgcaag gaggcagtga tgatgctaaa aataccgtcg
     1861 ctgaaacaac taaagaaagc gtgacagaag tgtcttctgc agaatcggta gcagaatcta
     1921 cagtcgttga agaagaaact gaaactactg aaactactat agaagaagtt actcaagaag
     1981 aaagtgttcc tcgtgaatac agaaatgcat tgagcaaagc tgaaagctat ctaggttggg
     2041 ctggtatgtc tgaacaaggt ttgcgtgaac aactagagtt tgaagaatat ccaagtgatg
     2101 caatcgatta tgcgctggct aatgttgatg tcgattacaa cgaacaagct ttggctaaag
     2161 cggaaagtta cgatgattgg gcatcaatgt cagattcgca attgtacgat caacttatat
     2221 ttgaaggttt tacaaatgag caagcacaat acgctttaga taacctacca caataactaa
     2281 caaaaacacg cccctccctc gccaaagttt gtggacgtga tgaaaaataa acctgctata
     2341 atcggcttac ttatctattc ctattatagc aacaaatagg agatgaaaac aatgtggatc
     2401 gaaactaaaa ctgataaaaa cggaaaaaaa gtatataaat ataatgagcg atatattgat
     2461 ccaaaaacta gaaaaagaaa aaaagtgagc attacttata aaaataaatc tcgagaaact
     2521 cagaaagtgg cattgctaga gttaaataaa aaaattgata taaaattaaa cgaaaaaaca
     2581 cttcataagc ctgatctaac atttcatgag cttgttgaag aatggttagt tatatacaaa
     2641 agacaagtta aggaatctac atattatcct acgaataata tattaaacac tataaagaag
     2701 aagatacctg agacttacat cgtttctggt attaatacaa tagatttga
//
LOCUS       DAZKLA010000199         2619 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000199 DAZKLA010000000
VERSION     DAZKLA010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.206"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..317)
                     /locus_tag="ACG85N_002791"
                     /pseudo
     CDS             complement(1..317)
                     /locus_tag="ACG85N_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            complement(349..672)
                     /locus_tag="ACG85N_002792"
     CDS             complement(349..672)
                     /locus_tag="ACG85N_002792"
                     /inference="COORDINATES: protein motif:HMM:NF015824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0225494.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTHFGSVAKFSK
                     KSILV"
     gene            1134..2516
                     /locus_tag="ACG85N_002793"
                     /pseudo
     CDS             1134..2516
                     /locus_tag="ACG85N_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          940 a          456 c          433 g          790 t
ORIGIN      
        1 tttcgtaacc agatatctaa ggttaagccg tccgcatcaa ttgcacgata aagataatgc
       61 caacgtccct taattttgat ataggtttcg tccattttcc atgaatagaa ggattgtcta
      121 tttttcttct tccaaagata atagaggact ttgctgtact cttgtaccca acgataaatc
      181 gtagtatgac aaacatttat tccacgatca tatcacaatt cctgaacttc acgatagctt
      241 agattgtaac gcaggtagta accaacagcg acaataatga cgtctttttt gaattgtttg
      301 cctttaaaat gattcattac tctgtcctct ctgtcttttt tctcaatttt acactaaaat
      361 agattttttg gaaaactttg caacagaacc gaaatgagta tagtatgaat tagtaatttg
      421 gttttcatct aagtttagct ttgatttact aaaattgata tctcgataca tgttcaatac
      481 atctaataca aatcgagaat cttctttcgg aaatggctca gaaagatcac tccaaatttc
      541 atcatcataa tgatactcgt aaccttctct caatatttcg atttttcttt cataataaga
      601 tctgttttgg tcctcaaatt tagccaataa ctcaaattga tttactagtg acaatctctg
      661 aaaactgtcc attaaatgtt tcttcctatc ttgttcatta acaaaaaatt ataagccctt
      721 acaacgaata ttatatatta aatttaattg taggtgaaca cgaattttat aaatatttat
      781 ggtgttaatg acaaatgata tccgactcct cttcatacgt tctggactat ttgctaatag
      841 attaaaattt cagcatctat ttctgaccat aaataaatga gctgccgaaa agctgccaaa
      901 tcagacttga aaataaagga tcaaaaactt ttaagtttct gaccccgtaa ctcacacttc
      961 aaaatcttac ttaaaaagga gtctgaaaga aaaaaagagt attcaaaatt tttttctaac
     1021 tagctacatc acatctatgc attgacactc aaagaaccag aataagtcat cagaaagcaa
     1081 gggggtgcgg acataatcct ggacgaaaaa ataaacttag gaggaatgtc cgtgtgtttt
     1141 cccacaaaga gcgtgtaagg gcaattcagt tatttttaaa atacgattgc tcttatgctg
     1201 caaccattcg agaattaggg tatccgtcga ttggtgcact acggaaatgg tataaagagt
     1261 atttggaatc cggagagctc catcaggagc accgaaaaaa atcaaagtat tctgaagaac
     1321 aaaaacgaat agcggtgaat cattattttg aatatggtca atgttatgca cgaaccattc
     1381 gcatgcttgg ttatcctaat agagagtcct tgcggcagcg gtgtgaagaa cttgcaccag
     1441 gagctagaaa actccgaaaa agtgcggtaa agttaacaca agatcaaaaa gaagctgtgt
     1501 tgaaaaaatt ctataaaccg caaactaata ggaaaaattt agccgaatct ggaggaatta
     1561 gtagagttac tctatatcaa tggaaaaaca cggttcttgg aaaggatttt ccattgtgta
     1621 tgactttaaa aaatcaggaa aaacaaaaag agctactttt aaaggaagta gaagacttac
     1681 aaaaacaagt acaccaatta cagctcgaaa aagcattact cgaaggagcg gctgaactgc
     1741 taaaaaaaga aaaaggcgtc aatcttcttt gtctatcaaa tcaagaaaaa acgatattga
     1801 ttgatgcctt acgaaatcag ttcaccttga aagagcttct tcaacagctc cagtttccta
     1861 aaagcagcta tttttatcaa aaacaagcac ttgagaaacc agataagtat tacaaagaac
     1921 gtcaaattaa ttatcacgat tttcaatcat aacttctgtg cgtatggtta ccgtagaatt
     1981 caccaagctt taaaaaatat gggtaaaaag ctttctgaaa aagttgtacg tagattgatg
     2041 actgaggaaa atctatttgt taaattttct agacgaaaaa agtattcttc gtatgctggg
     2101 gaaatttctc ctgcacaccc caatttatta aatcggaatt ttaaagcaaa gaagccaaat
     2161 gaaaaatggt taacagatat tactgaattt agaattctcg cagggaaaat ctacttatca
     2221 ccacttgtcg attgttacga tggtgctatt gttagttgga ctattggaac aaagccaact
     2281 tgcttcactc tatgtctaaa aagagttatt caccagataa ttcagcgtgc gaaggtttct
     2341 ttggacgatt aaaaaacgag atgttttatc aacgtgactg gaagaataca acaatcaatc
     2401 agtttataaa caaattggat gattatattc attggtataa taatcaacga attaagttat
     2461 ctttaggaag gctaagccct cttcaatatc ggaagaagca aagatatatt tgttaaaaag
     2521 gtccaaaaaa aatatccgca cccccttttt ttgtaaaaaa ctattgcaca atacaagtag
     2581 cctacggcta tcgaaatttc cataactttc gagcaagaa
//
LOCUS       DAZKLA010000200         2606 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000200 DAZKLA010000000
VERSION     DAZKLA010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.207"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..189)
                     /locus_tag="ACG85N_002794"
     CDS             complement(<1..189)
                     /locus_tag="ACG85N_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0225495.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYM"
     gene            complement(183..584)
                     /locus_tag="ACG85N_002795"
     CDS             complement(183..584)
                     /locus_tag="ACG85N_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0225496.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(771..2579)
                     /gene="uidA"
                     /locus_tag="ACG85N_002796"
     CDS             complement(771..2579)
                     /gene="uidA"
                     /locus_tag="ACG85N_002796"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFQ0225497.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
BASE COUNT          816 a          522 c          329 g          939 t
ORIGIN      
        1 catatattca tctacatagg taacgaattg acctacctcg ttaaatccat ttgtcaaaca
       61 taacttagcc atataagaat gataatgttt gagtatcatc ttgatcgaaa ttggatctcc
      121 ttctagagca gataagataa tggatagtgg aactaatagg tactgccgct ttttagattt
      181 atctaccata ggtcataccc cattttcaat tgttcaactg ccttctttcg ttgataccag
      241 ataccacttg tgctcatatt gagaatttta ccgatttcag catcattaaa acctgcaaaa
      301 taataaagta ggataatctt tcttcttatc tccgataact gatcaattgc tgctgataac
      361 ttttgatctg aaattaatat ttccattcca aataagaaat agacatcttg ttcatcaaaa
      421 tgagtttggg aatacctgcc ttcatttgaa tctgccaatg gttcctcatt atcctttatg
      481 ttagcgtttc gttttcgaat agacttagct tcgtttcgta atactttttt acagtaggca
      541 tcaaattggt gttgtataga tatgtcattg taatgttgtt gcatgataat atgctccttt
      601 ttcccccttt ccactaatac aacaatcaga aagccccttt ttttcgaaaa aactagaaaa
      661 aaaacataaa aatatttatc attctttaaa cagaaaatct ttctataaaa aacaaaaaaa
      721 aacgattagg ctaggctaat cgttttttga acattcttaa aaaatccaaa ttatttttta
      781 taactgaaat tgggtattga tgaccatcgt ttctgtagat agtaagcaat cattttaggt
      841 tttctatctc tagtaaagat tcctttctta tttccttgca ctcttaaaat cccttgactt
      901 gttgcaaaat ctgcaaaatt ccacacttgt tcaccaacaa aattttcaca tgtatctatt
      961 acttcgtgat ttgcttcgta ataagccact tgatactctt ctgtaaacat tgttggtact
     1021 gtatcatgaa atccagctac tgtatctgct ccatattctg taaacattat tggcgtatca
     1081 ggacatcttt ccatccatcc atctagctct tttttcaaca gatattttgc atgtgccaga
     1141 tcccctcccg aaacatacca accataataa cgatttaaac ataaaacatc gacaatatct
     1201 cctaccttac aaacatctgg cgttgatttt agataagtaa cgattgttac aggtcttttc
     1261 tgcggatcaa gttctttagt taattcaact aaaggttcaa aatattcttt agcaccttcg
     1321 gaatccgaat ctggttcatt tgcaatagac cacattacaa cacttgggtg gttcttgtcg
     1381 cgttctatca attccttgat caccgtttta tgagaatttt ctgttcgtat ctctttccac
     1441 gtattatgtt tttccacatt atcatctaat aaagtggcca tgaaatttaa atgtaaccct
     1501 actgcaggta cttcatcaat aactacaata ccttctcgat ctgctaatcg cataatttct
     1561 tctgagtaag gataatgtga tgttctaaaa gaattagcac ctatccattt cattaaatta
     1621 aaatctttaa tatttacagc ctcattcaaa ccacgtccat taacatacga atcctcatgc
     1681 ttaccaaatc ctttaaaata aaaaggttta ttattaatca aaaatttgcc attggtaacc
     1741 tgaacactgc gaataccaaa ttcttctgta taggtatcga tcactttatt atcatcaatt
     1801 aaactaattt tcatactata taaataagcg tctagaggtt cccaaaaaat tggattctta
     1861 agctgaatca ttcctttctt tccttcagct tcagctacaa gttgagcctc ttcgtcatat
     1921 atcgctatta agatttttcc ggtccattct gtatcgatat tgaattctac acttgcatcc
     1981 aaatcagtta ttcttgtatt aatagtaata tcacttatat agtctttcgg agttgtatat
     2041 aacttaacag gtctatgaat acctgcataa ttgaagaaat caaaattaac taagttttcc
     2101 ttttttatat ccccattttc ctgtactgtt tccttcataa tccccacagg taaagtacta
     2161 tcgtcaataa tattgtttac agcaactgta attcgattat tcccgtcttt tacaaaccga
     2221 ttaatttcag actcaaatgg tgtaaaccca cctttgtgtt gagtaataaa ctttccattc
     2281 aaaaaaactt ttgcttcatg tgttgcagat ccaaaacgaa gaactaatct ttcttctaat
     2341 aatcttttag gaagagtcac ttctttttca taccacaccc atccaacatg atttctaatg
     2401 ttctctgttt caaataaatc attataagaa gagggaactg aaatcggata actctctttt
     2461 tcatttattg gttctaaagc aagttcttcc tgtaagtcat catttgtatc taatttaaat
     2521 ctccagagac cgcttaagtc aattacttgc ctcgtctctg tcataatggg atacaacata
     2581 taaaccactc ctatctaaat attaaa
//
LOCUS       DAZKLA010000201         2571 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000201 DAZKLA010000000
VERSION     DAZKLA010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.208"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..390
                     /locus_tag="ACG85N_002797"
     CDS             <1..390
                     /locus_tag="ACG85N_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0225498.1"
                     /translation="NLIDYLSPIENIRLVNKSADESILFELGLDKKQIKRNVMKLSGG
                     QQQRVAIARALVSDAPIILADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKE
                     VADSADIILELSGKKLKKVNKMNLEVE"
     gene            390..1766
                     /locus_tag="ACG85N_002798"
     CDS             390..1766
                     /locus_tag="ACG85N_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0225499.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            1896..2309
                     /locus_tag="ACG85N_002799"
     CDS             1896..2309
                     /locus_tag="ACG85N_002799"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0225500.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIADCRQKLFQTRNSSLEQLFIYFVSNF"
     gene            complement(2334..2571)
                     /locus_tag="ACG85N_002800"
                     /pseudo
     CDS             complement(2334..2571)
                     /locus_tag="ACG85N_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          957 a          355 c          461 g          798 t
ORIGIN      
        1 aatttaatag attatttatc accaattgaa aatattagat tagtaaataa atcagcagat
       61 gaaagtatct tgtttgaact aggtttagat aaaaaacaaa taaaaagaaa tgttatgaaa
      121 ttatctggtg gacagcaaca aagagtagct attgctagag cattggtatc agatgctcca
      181 ataatactag ctgatgagcc tactggtaat ctagacagtg ttactgctgg agaaataatt
      241 aatatattaa agacattagc taaagataga aataaatgtg taatagttgt aacacatagt
      301 aaggaagtag cagattctgc ggatatcatt ttagaactaa gtggtaaaaa gttgaaaaaa
      361 gtaaataaaa tgaatttgga ggttgaataa tgataaaaaa tgcatttgct tatgtaacta
      421 gaaaaagttt aaaatcatta attatcatat tagttatttt atctatgtca actttaagtc
      481 tcattagttt atctattaaa gatgctacgg acagagcttc aaaagaaaca tttgctaata
      541 taacaaatag tttttctatg gagataaata gacaagtaaa tccaggaaca cctagaggtg
      601 ggggaaatgt aaaaggtgaa gatattaaaa agatatctca aacagatagt atagactcct
      661 atgtaaaaag aataaacagt gttgcagatt tagttgatta tgatattatt gaaactcaag
      721 agactcttgc gaatcaatca cctgaaagag caaagaattt taaaagaaca gttatgttaa
      781 ctggggttaa cgactcaaca aaagaaacaa aatttgtatc agaagcatat aaattagtag
      841 aaggaaagca tttagaaaat gaagataaaa ataaaatctt aatgcataaa gatttagctg
      901 aaaaaaataa ccttaaagtt ggagataaga taaaaataaa atctaattta tttgatgctg
      961 ataatgagaa aggtgcagat gaaacagtag aagtagaaat taaaggtcta ttcgatgggc
     1021 ataatagcgg tgtagtaagt gcagcacaag aactatacga aaatacatta atcactgata
     1081 ttcatacagc tgctaaagtt tatggtaata ccgaagatac agctgtatac caagatgcaa
     1141 cattctttgt aaaaggtgat aagaatcttg atagtgtaat aaaagatctt gggaaattag
     1201 atataaattg gagagaatat aatttgatta aaagttcatc aaattaccct gcattacaac
     1261 aatctatatc aggtatttac tcaatttcaa ataaattatt ttttggctca ttaatatttg
     1321 caggagttgt agtttcatta ttattattct tatggatgaa tgctagaaag aaagaaatag
     1381 cagtattact atcattaggt atatcaaaat tagaaatttt tggtcaattc ctaattgaga
     1441 tggtatttat atcaatcccc gcattcgttg gttcctattt cttagctcaa tatacagctg
     1501 acaagcttgg gaataatata ttgaataagg ttactggtga tatagctaaa caaatagcta
     1561 aacaatctgc atctagccaa ttaggtggtg gggcagaagc tgaaggattt aacaagacat
     1621 tatcaagttt agatatcaat gtattaccta aattcatgat ttatgtagtt atatttatga
     1681 gtttagtact tcttgtatca ttgattattt cttcattcaa tatattaaga aggaatccaa
     1741 aagaattatt aattgacaat aattaaaatt ttcggtgctt tttagcacca aaaatgagag
     1801 tgtaatataa accgtgtaag taaccatcaa tcctaggcgg gtggttgctt cacggttttt
     1861 atttttctaa aataagatca agaggtgatt acacgatggc tagaaagaaa agaaatcctg
     1921 atgctgagaa gttagctgaa tccattttga atacttatca acccgaatct gtagaggata
     1981 tgcaagatgt tttaaaagat gtgtttggtc ccttatttga aaaaatgctt caaggagaat
     2041 tgaataacca tttagggtat gacgcccatt ctaaagagcc aaagaaacat gacaatcgac
     2101 gaaacggtta tggcaataag acgcttaaaa cgagctttgg taaagtaaac attgatgttc
     2161 ccagagaccg ggaggcttct tttgaacctg agttgattcc taatagaaaa cgagatgtct
     2221 ccgacatcgc agactgtaga caaaagctgt tccaaactcg taactcgagc ttggaacagc
     2281 tttttatcta cttcgtttcg aatttttgaa caaaaaaata aaggctcctt gcctttccat
     2341 agccaggtgg ctaatttctt taaattcatt gcagcaaaca ctagcatcgt atgcgtcgcg
     2401 actttttcca agcctcgata cttggtccaa cgcatcccat gcttttcttt tgcatccgca
     2461 aatattcgtt caatcgtttg tttccgtttt ttgtaaattg atttattcag atctgttagg
     2521 cgaagatgtt ccacttcatc catcagatct ccccaaacat gtctcgtgat g
//
LOCUS       DAZKLA010000202         2558 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000202 DAZKLA010000000
VERSION     DAZKLA010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.209"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..962)
                     /locus_tag="ACG85N_002801"
     CDS             complement(<1..962)
                     /locus_tag="ACG85N_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0225501.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHT"
     gene            1134..1268
                     /locus_tag="ACG85N_002802"
     CDS             1134..1268
                     /locus_tag="ACG85N_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225502.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(1261..1896)
                     /locus_tag="ACG85N_002803"
     CDS             complement(1261..1896)
                     /locus_tag="ACG85N_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225503.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(2009..>2558)
                     /locus_tag="ACG85N_002804"
     CDS             complement(2009..>2558)
                     /locus_tag="ACG85N_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFQ0225504.1"
                     /translation="KKTPMILLIIGIIFTTVGFSLSSSNEANDNNKQESSSSDTQTTT
                     SNSSNKSLTQFEEFLETNNHDWGAFLDSYYSITPATEQNTAFTKYISGKTYTFEGTVI
                     ESMTTRIAIIADKEYDDKSWNDISATPKVSYVIFAKDLNNADTFTKGDKVTFTGEISS
                     RGSNIEKSYAQWDMINSKVSKK"
BASE COUNT          720 a          430 c          393 g         1015 t
ORIGIN      
        1 gtatgcctta gtccatgcaa agtaaattgc ttttcaatcc ctaattcttt taattttttg
       61 cgcaaatatt tgctgacggc gtttgatgaa actaatccat tttttaagtt gaaaaaaaca
      121 aaattatgag ggtttttgat ttcaaaattc tcgaataatt ctttttgatt gattttaaac
      181 ttttttaaga gatcaatcgt atgtttatca atggaaattt tcctatttga agtttcgttt
      241 tttgtttttc cccattcatt taatttataa ttccatgttt tattaatagt gatgatttga
      301 tcttcgaaat cgatatcttc ccatgttaat cctagaagtt cagcaaatcg cattccagtg
      361 gcaccagcaa ctaacacgag cataggagaa gagtactgcg cagaaaggtt ttcttctacg
      421 agtttcatta aagatttaaa ttgatcataa tctaaatatt tatcttcttt tggcttcaat
      481 gaatcttttc ctttgattac tgcttttcta gttggatcaa acggaattaa tccttcttct
      541 acggcatctt tcaacgacgc tctaatatgg ttattaaact taataacggt agattttaca
      601 tggtctttag catacttatt taaaaagcgt tgatatccca ctctatctaa atcagaaatc
      661 aataccgctg gcatatattt ctttatgttc attaacgtat cttcgtattt tctataagtg
      721 ataggagata ctgttccttc tttataaagt tgcatccaat cctcaaagta atctgataat
      781 aatagatttt tgcgatcccc tttaaggcct ttgttcaagt tatattctaa ttcgttggcg
      841 gcatcttttg cttctccttt tgtcttaaat cctgattttc ttatcttcgt gtattttccg
      901 tcatcttttt tgtatgaaat ttcgtattgc caactatttc cacgtttgac taatcttgcc
      961 ataattgaat cagctctctt tctctgatac aataggaact ataaagaagc ccattgtttg
     1021 ggttttatta ttttttgcac atcctttttg cttgccggcg caaggatgtg ctatttgtcg
     1081 ttttaatggt ataattggat gtgtattaga tcatccaatc agaggtgttt attatgatta
     1141 aagtgatttt taaaagaata actgaacttc cagagtttag attatttgtt gattattggt
     1201 atgtctggtt ttttattgtt gcattgctga taggtctaga tgtcatatta gaaagaaaac
     1261 ttaagtaaga gttgagttaa taaattgaat tcctaaatga gatattttgt agcccatatt
     1321 ttgtttgccg acatcaccta atactatgga gctaatcgtt ttttctaaca gaccaaaatc
     1381 agataatcta tttaagctac catctattag ctcttgtgaa tagccagaca tgctgatatt
     1441 tccagtgaaa ttttcgtttc cacgactcat taagcatgct aacaattgaa tctcaatcga
     1501 tgttaattga ttcagtacaa gaagataata ctcctcgtta tcccatgaag cgttgttaat
     1561 tcgaagtaaa caattcttat aaagattttt atacatatct attttatttt gtgcctttga
     1621 tttttctacc tcgtcattta tcccttcgaa tattccaatg agttggtcct tgttctctaa
     1681 gttgaaaaca tcttctggaa tttgatcgcg aatgttttct aatgattcgt ttaaattttt
     1741 gtagaatttc tcgatgcgtt tgaaacgctt ctcgttttta gacccaaaat aaagtgactg
     1801 cacaacgcca ccaatataag gaattgctcc aataccgcct tcaactatta attcaagata
     1861 ttcttttttg gttagttttt cattgctatt atccatagtt acctcctatt ttgtcatttc
     1921 ctttacaagc attgtgactc ctgatggcac tcttttgtat aggttttatt attgtgcaca
     1981 atctaactct tagtggggcg tggttcattt attttttact tactttacta ttaatcatat
     2041 cccattgagc ataggatttt tctatgttcg atcctcttga actaatttct cctgtaaaag
     2101 ttactttatc gccctttgta aaggtatcag cattgtttaa gtccttcgca aaaattacat
     2161 atgaaacttt aggtgttgca gaaatatcat tccaactttt atcatcgtac tctttgtctg
     2221 ctataatggc tattctagta gtcattgatt cgataacagt tccttcaaaa gtatatgttt
     2281 taccactaat atatttagta aaagctgtat tttgctcagt agctggagta atagaataat
     2341 aactatctaa gaaagcaccc cagtcatgat tatttgtctc taaaaattct tcaaattgag
     2401 ttaggctttt attgctgctg ttagatgtag tagtttgagt atcagatgat gagctttcct
     2461 gtttgttatt gtcatttgcc tcgtttgatg aggaaagcga gaaacctacg gtagtaaaaa
     2521 taatcccgat aataaggaga atcataggtg ttttctta
//
LOCUS       DAZKLA010000203         2488 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000203 DAZKLA010000000
VERSION     DAZKLA010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.210"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..475
                     /locus_tag="ACG85N_002805"
     CDS             158..475
                     /locus_tag="ACG85N_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFQ0225505.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            472..633
                     /locus_tag="ACG85N_002806"
     CDS             472..633
                     /locus_tag="ACG85N_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0225506.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            636..935
                     /locus_tag="ACG85N_002807"
     CDS             636..935
                     /locus_tag="ACG85N_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225507.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            935..1249
                     /locus_tag="ACG85N_002808"
     CDS             935..1249
                     /locus_tag="ACG85N_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFQ0225508.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            1242..1772
                     /locus_tag="ACG85N_002809"
     CDS             1242..1772
                     /locus_tag="ACG85N_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFQ0225509.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            1769..2038
                     /locus_tag="ACG85N_002810"
     CDS             1769..2038
                     /locus_tag="ACG85N_002810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225510.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            1968..2171
                     /locus_tag="ACG85N_002811"
     CDS             1968..2171
                     /locus_tag="ACG85N_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFQ0225511.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILSDDRKWEEKE"
     gene            2168..2455
                     /locus_tag="ACG85N_002812"
     CDS             2168..2455
                     /locus_tag="ACG85N_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225512.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
BASE COUNT          930 a          363 c          580 g          615 t
ORIGIN      
        1 tctggagggt tttaaaaaaa taataaagta ttaaagtaaa aaataaaaag taaattatat
       61 aaaaaagaat aggtttttgg gtctgaccct ccaaactctc caccaaaatc ttcctaaatg
      121 ctttgatacc aacgattaaa cagtctggag ggttacattg aaatcagaac atcaaataca
      181 agacgagatc agaattgcct tatcaaaaca tggttgcacg atttttcgat ccaatgcggg
      241 acaagtgaca acgatcgaag ggcggcaatt ttacgggatg ccaaaagggt tcccagatct
      301 atgtggacat cgtaattctg atgggaaagc aatttatata gaagtaaaaa atgaacgcgg
      361 taagttgcgt gaagatcaaa aaaggtttgc tgaattttta aacggtcaac ctgtactata
      421 cggtgttgca cgatcagtgg aagaagcatt gaaaattgtg gaggaatcat catgacaaca
      481 gaagaagtga ttcaaatgcg tattcgaagc cttcagcgtg agattgacga tctggaacgg
      541 acaaaggcag tgatggtcaa tgaaacggcg aggaaggcaa tcgatttgca catagagaat
      601 ttaagaaggg aaatccatcg attggaggaa tgagcgtgga taagaaagca gcaatgaaac
      661 gaatcatcga actgacacat tctgagaatt ggcaagaaga caaagaaata gttgcagaag
      721 tccaaaagct cggtaaatca atgtggagtg aaaaaaccaa acggagaaca ccgagaaaaa
      781 ttgcaatctg gcatggtgac cgaattctag taacaggtac tgctgaacag ttatctgaaa
      841 ttactggatt aagcaaaaac attatctggg atagagctaa acgcgagaac gttgattcga
      901 aaggtcgtca atttaaacat tgggagaaga aataatggag gaactaatca caaaagtaga
      961 gcagtgggca aaagataagg gattggatca agctgattcc agcaagcaaa tgttaaaaac
     1021 gatcgaagag attggggaag ttgccgcttc tctagctaga aaagatgaat atggtttaag
     1081 agacggaatt ggagacgtag tagtaacctt gattatttta gctatgcaaa atgatatgga
     1141 tttgtacgag tgtctgaacc aagcatacaa cgaaatcaaa ggacgcacag gaaaaatggt
     1201 agatggtgta ttcgtgaagt cgagtgattt ggaggacagc gatgaataaa aaagtattaa
     1261 ttgataaaca agcattgatt gatgaattaa tgaaaatacc tggtgtggga tctaatagtg
     1321 acgctttaga aacgattaaa cgtttcccat cgtatgaacc gcagaagcca gttgtgccta
     1381 agtttgtggc ggaactactc gactattatc gtcagtcaac agatgttgat ttattagctt
     1441 tattgataac tttccatgat tggtattatc gcaaaactaa agacggtgag catgaagaag
     1501 caattgattg gctagttgat catcctgaaa tttatatgcg tgcatggctg tttggctacg
     1561 agctcgagaa agagccgttg tattgcgtaa taatcaatgg tagatatctt gtcaaagtat
     1621 tcagcaatac aaacgttgtc atattagttc cagcagatga attttcacag tacgttactc
     1681 aaaaatttca attaactgaa aaacaaatca aagcaattga tgaaagatac tggccgtttg
     1741 ctgtgccagt ggatgaggtg gtagaaggat gagcgaccca tacattacta aattagaaaa
     1801 gttgtccgaa gattttgaga agaataaaaa taaactaact aaagataaac ttatagaact
     1861 ggttaggttg gttatcagag gagctattac aagagaaaag gcgcagcaaa acatagaaat
     1921 ttttgctcgt agtgtccgag atggattaga gaagaaagtt aaacaaaatg aacaacagac
     1981 accgcagaat aacaaaacta agaaaacggg aactgaatgt actaaagaca aagtttgaaa
     2041 aagaatatgg aatttcagca gaagaaacat ataaagtggc aagtcagtgt gttgctgatg
     2101 cgagcggcgc tattcgtaag tttggaattt cgatattaag tgatgatcgt aaatgggagg
     2161 aaaaagaatg aaactaaaag acggatttta ctccagcagt catggtatcg gcggtttaat
     2221 gctagatatg ccgacaaaga accctaaaac acgtgagaaa ccaaaattca aagtcggtga
     2281 catggttcgc tgtgaagcag aaggattcat ctatccattt tgtggatatg tagaacatct
     2341 ctataatcac tcagcaatca ttcgtattga aaacacgatg gaatgtgaca agtggttagc
     2401 gaaaagcaaa gagaatttag cagtagcgag attggtggat atggaggtta tataaacagc
     2461 gaaaaaatgt acttctaaga gaaaaatt
//
LOCUS       DAZKLA010000204         2421 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000204 DAZKLA010000000
VERSION     DAZKLA010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.211"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            121..2274
                     /locus_tag="ACG85N_002813"
     CDS             121..2274
                     /locus_tag="ACG85N_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFQ0225513.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          827 a          425 c          536 g          633 t
ORIGIN      
        1 aaaagttgat tttatcttga aattaggtaa agaatttctt gctccgccca ttttttcttt
       61 tttattaaga agtttttggg tacaatgaag catgaatgta aataggcgga aggataggaa
      121 atgatgaaag attttatgaa aaaaatatta aatacttctt ggttagaaaa aataagaaaa
      181 ggtgcacaag aacctaagaa caaaacgttt cgccggtccc atgttccttt tcgcttaaat
      241 tttctttttt tcattatatt tacgttattt gttgcgttga ttgctagatt gggttatttg
      301 cagatcgtga acggcgaaga gatggaggcg cgagtgaagt ctacttctac gattacggtc
      361 caagaaagta ctcctcgcgg aatgatctac gactccacag gaaaagcttt agttacgaat
      421 aaagcgaatc aggccatcac atatacgaga ggaacacaag ttacggctaa agatttgctt
      481 gaaatcgcaa cgaagctgaa ctcgctgatc gatgttccgg tagatgaaaa tctgacagat
      541 agagataaaa aagatttttg gctggctgat cccaaacatt tgaaagaagc cacaaaaaga
      601 ctttcggcaa aagaaaagca attagggaac agcgaacaat atagtgcgat ggtcaataaa
      661 gtcacagctg atgagattaa tttaaacgag gagcaattaa aaattgctac catctttaaa
      721 cggatgaata gtgcatatga attaaacact gtctatatca aaaactctgg tgttactgat
      781 caagagttag ctgttgtggc agaacgcgct tctgaacttc caggagtttc gactggcaca
      841 gactggacac gagaatataa tgcagcaagt tcactgaaaa gtattcttgg cagtgtgacg
      901 actgaaaagc aaggacttcc agcagatgaa gcagagaaat acctagcaaa aggttattct
      961 cggaatgacc gtgtaggaca aagttatttg gaaaaacaat atgaagatgt cctgcaagga
     1021 accaaaaccc aatatgaagt ttcccttgat aatgaaggaa atgtttcgaa tcaaaaagaa
     1081 atcttctctg gggaaaaagg ttctaattta atgttatcta tgaatgcaga attccaatca
     1141 aaagtagaag aaattttgaa aaggaattat caaacattga tcaacaacgg caaagcacag
     1201 tattcgcctg gtgcttatgc agtggctatg aatcctcaga caggagaagt gctagcaatg
     1261 actggtttca gtcatgaaca aggttccaaa gaaatcaccg agaatgcttt aggcacgatt
     1321 actagcgcct ttgatccagg gtctgtagtc aaggctggta ctttgacagc aggctgggca
     1381 agtaacgcga tcagcggaaa tcaagttttg attgatgagc cgattcgtct gcaaggtgct
     1441 tcagaaaaaa gctctgtctt taatcgaagc ggacaagttg ctcttgatgc agtcaaagca
     1501 tcggaattgt cttctaatac gtatatgatc aaagtggcgt taaagatgtt aggactagac
     1561 tatacacctg gaatgggact acctagttta gatgaagaag ctaaagctta tcagcaattg
     1621 agagactctt tcaaagagtt tggtcttggt acaacgactg ggatcgattt gccgaatgaa
     1681 tctcctggga tctcacgttc tgttgattat atgaagaagt tcaatgcgga taatggcaaa
     1741 gaatggtatg ctccaggtaa ctttaccgat ttggcatttg gacagtttga cacatataca
     1801 ccaatccaat tagctcaata tgcttcaaca gtcgctaatg gcgggaaacg agtacagccg
     1861 cacctagtca aagcgatcta tggcaatgac gaaaatggaa acttgggtga agtgaagaaa
     1921 gaaattggca caacagtaga aaatacggtc aatatttctg cagaaaatat ggcgatcctc
     1981 cgtgaaggat tccaccaagt tgtccatggt accgatcctt acacaacagc caaaccatta
     2041 gccagtgcga aaatggacct ttccgcaaaa acagggactg cagaaacggt tgcggaaggt
     2101 catccagata ttgcgacagt caatagtaat atcgtggcat acggaccaac agacaatccg
     2161 gaaattgcca tcagtgttgt cttgccgaac ttattagacg aacaagacca cataaacttg
     2221 acgattgcta aagaaataat ggacgcgtat tatgatatgt tcatggcaaa ataaaaaaga
     2281 ggtcgtgaaa aagccgctta gtttcgagta tgaagaaaaa ttttgtgaaa atagcttctc
     2341 acatttttgc ttaaatttga cttcataccg agaaactggc ttttgaacac cgtttactcg
     2401 gcaaaaaagg ttgtgacaaa a
//
LOCUS       DAZKLA010000205         2404 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000205 DAZKLA010000000
VERSION     DAZKLA010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.212"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(4..249)
                     /locus_tag="ACG85N_002814"
     CDS             complement(4..249)
                     /locus_tag="ACG85N_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225514.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(266..1117)
                     /locus_tag="ACG85N_002815"
     CDS             complement(266..1117)
                     /locus_tag="ACG85N_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0225515.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1283..2287
                     /locus_tag="ACG85N_002816"
     CDS             1283..2287
                     /locus_tag="ACG85N_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFQ0225516.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT          773 a          405 c          485 g          741 t
ORIGIN      
        1 tttctatttt ttcggtatca aggcaaaaac aggtagttca ggtgaataaa tgacttcatg
       61 ttcgtgccag ggtttgacca ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat
      121 ttgatcatgt gtccagtttc ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa
      181 gacaaaatcg gaatgaaaag tctgggtaac ttcttccgag agagaattca agcgttcgat
      241 tggatccata aaatcacctt tcgacttaaa attgggtagt atctgggtct tttgctttgc
      301 cgactacacc atctaactga tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa
      361 ataattcagt attttctttg attctgcttg gtgtcactga cttaggaaga ggaaggaatt
      421 cgtgttgcag cgaccaacgc aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga
      481 tttgtttcat ttcaggcaca tcgaagattt taccagtgcc taatggacta tatgcttcga
      541 ggatcatatc atgttttttt gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa
      601 ggaaaacttg attgaccatc ggcataattt ttgctgtttt tgctagtgta tctagatggt
      661 ggggtaagaa attgctcaca ccgattgctt tgattttacc tgcttcatac agttcttcaa
      721 atgctcgcca tgtatctgca tttgcttgtt cccaattatc acgaaaagca acagggtttg
      781 gccagtggat caaaaacaga tctaaataat ctgtttggag cttcttcaat gattcttcaa
      841 aagaagacat cacaagttca tagctgtggt ttgcattcca taattttgtt gtcaagaata
      901 tctcttctct aggaattcca ctttccttaa tcgcttggcc gacgctttct tcattcttgt
      961 atccttgggc agtatcgata tggcgataac ctgcagccag cgcttcttta acagaggaaa
     1021 cagcgacatc cccatcaggt gtttgccatg tgccgaatcc tactaccggg atttcgtaac
     1081 catttgatag acgataagtg cttgttaatg aattcatact ttcagctcct tttttattcg
     1141 ttcaactata agtgtagcgc atttttcgaa aaaaagaatc cattttgttt ctactcttga
     1201 aatttttgtt taaaagaaaa tgattttgat tctggtattt ttgatttttt ttccgtaaac
     1261 taagacagaa gggaatgaga aaatggatat ctatttaaaa aaagcaattc tgcatattat
     1321 tgacagagaa actggttctc cagtcttttc acaaaaagag ctggatctga caaaagaata
     1381 tattcgtgat tttcttcaaa agaagatcca aaaaatctct tctgctcaaa caaaaactgg
     1441 acaactagca gaagaaagca tgatcgttga atcacttaaa atcgctcaag aaaattttgt
     1501 ggaagcaagt gaagcaatcg ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc
     1561 acctagtgca gatgtatttg ttgtcttgta tgaattagat acagtgatgt acttatcttt
     1621 attgaaagtc aattatcgtg aggcttacac tcactttgtt gaagcagacg aagcagggat
     1681 cgacaaccgc ttgattttga atcgagcaat tctaggggcg aaaacacaaa aagcagatga
     1741 agcattcgct gtgaatttga ctgattggac ctacgaatta attgaaaaaa aatatgagtt
     1801 ttctggcaaa aaagagcttt atttttctgg aagagtgatc gaaagtgttc ctgcaccttc
     1861 tttagaagaa aatgtaaaag tgatccaaaa agtagccaag catcttggaa aaaaatttga
     1921 gacagaagaa tttgatatca tggctgattt gaaagaagct gtctatgata cgatagaaga
     1981 aaaaggtcgg ttggatcatg agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc
     2041 aaaaatggct tttaaagaag aagtacaaga gcaaggattt gtcccagaag cacctcctgt
     2101 aaaagaagta caagaaattt ctgagaaaaa atttggaaaa caaaaattca agctatcaaa
     2161 cgggatcgaa ctgatcgtac cagtagatgt atatcgcaac ccagatttga ttgaatttgt
     2221 gaataatccg gatggtacga tatccgtgat gatcaaaaat gtggatgaag tattgaatcg
     2281 tttataaaaa tgactagaat attaggtgtt caagtaccag tgatcagtta aatgaattgt
     2341 gtcaagagaa ggatcggaaa agacgaatat aacaaaaaaa gagcgtgaga caaaagtaaa
     2401 gaac
//
LOCUS       DAZKLA010000206         2398 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000206 DAZKLA010000000
VERSION     DAZKLA010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.213"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACG85N_002817"
     CDS             207..2069
                     /locus_tag="ACG85N_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0225517.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="ACG85N_002818"
     CDS             2069..>2398
                     /locus_tag="ACG85N_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0225518.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DAZKLA010000207         2375 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000207 DAZKLA010000000
VERSION     DAZKLA010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.214"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..586
                     /locus_tag="ACG85N_002819"
     CDS             <1..586
                     /locus_tag="ACG85N_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0225519.1"
                     /translation="IYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTD
                     YRNNYSAFKSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYIL
                     RYDDKIIEIHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMY
                     EKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            587..1339
                     /locus_tag="ACG85N_002820"
     CDS             587..1339
                     /locus_tag="ACG85N_002820"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFQ0225520.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            1344..2291
                     /locus_tag="ACG85N_002821"
     CDS             1344..2291
                     /locus_tag="ACG85N_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFQ0225521.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          837 a          306 c          459 g          773 t
ORIGIN      
        1 tatttatgtt gctgctccac tgaggtataa aaatatcatg gaagttgttc gtaatagtat
       61 tcaaagtaaa aaagtatatt ctgtaagagc tatttgttct acttatcttc ctgattggag
      121 aaaaggtact gattatagaa ataattattc tgcctttaaa tctatggggg gaggagtaga
      181 attagactgt atacatgaat tggattatat tataaaccta tttggtttcc ctaaatcagt
      241 aaaaaaacag tttggtaaat attcgaatct tgaaattaac agtaatgatt tagcaacata
      301 tatattgaga tacgatgata aaattataga aattcattta gattattttg gaagagttcc
      361 tacaagaaaa tttcaattgt ttactttaga tgaatgtatc aatgttgact tattaaataa
      421 taaaataagt tttgaagaaa acattgttaa agaaatgcct gaatctgcta atgatatgta
      481 tgagaaggag ttgaaatatt tctttgaaaa cgtagtaaca ggcaaagaaa actggaatga
      541 tttatatcat gcaaacgaag tattgaaaat agctgaggag aaataaatgg aaattttatt
      601 tactatttgt ggtagagctg ggtctaaggg gtttaaaaat aaaaatttga aatatttggc
      661 aggaaagccc ctagtccatt tcgctttagc aatgatagat atctatcaac aaaaacatcc
      721 tgaacataat tgcacgactt gtttgaatac ggacagtaat gagttagtag aattgacgac
      781 taagttaaat ccgtctgtga aaattgtaga acgtaaacct gaattagcta cagatactgt
      841 agggaaaatt gaagtgatac gtgattcatt cttacaacta gaaagagaag ataatatatt
      901 tgatttaatt atagatttag atttgacttc acctttacgt gaagtttcta atcttgaaga
      961 attaatagat gaacatctaa aaaatttaga taaagacgtc tacttttcag ttgtttctgc
     1021 aagacgtaat ccctatttta atatggtaaa aagaattgaa aatgaagtta ctatagttaa
     1081 taaatcaaga tacacatcta ggcaacagac acctgaagtt tttgaaatga atgcctcaat
     1141 gtattctttt aaacctgaat ttctaaaaaa aagtaattat atttttgatg ggcaatgtgg
     1201 gataataaaa atgagagatt atctaatttt agatattgat agtgaagaag attttaaatg
     1261 gttagaattt ttgtttccta aatttttaga agattcttcg gagcttcaag atatctataa
     1321 ttatttatat cggaggtaaa aggatgagct gttatcttat caccggtggg gctggcttca
     1381 ttggttctac cttagccaat catttaggag aagaacatgt ggtaatcgtc gtcgatgact
     1441 tatcaatggg aaaagtagaa aatcttgata tgacgaaaaa tatcactttt attgagggag
     1501 atgtcgctga gaaatccttg atggaagaga tcatgaaagc taataaattt gattatatct
     1561 ttcatttagc agcagttgct agtgtggcag attcagttgc ccgaccagta gaaacacatc
     1621 gtgtgaattt tgaaagtgta ttaatgattt tagaattgat ccgcaagtat caacctgatt
     1681 taaaacggat cgtgtttagt tcttcagcgg ctgtttatgg ggatgaaccg actttaccaa
     1741 aaaaagaaga gtccgtgatt cgcccattga cgccttatgc ggttgataaa ttcgctgcag
     1801 aacaatatgt gctagattat tgtcatttat atgatgtacc cggtagcgca gttcgttttt
     1861 tcaatgtcta tggtccaaat caaaatccga attctccata cagtggtgtg atttctattc
     1921 tcgtagatcg ctataagaaa caaatcgtag gagaagcaac ttcatttaca ttatttggtg
     1981 atggaagcca gtctagagac tttgtttata tagatgatgt aatccaagca ttacttttag
     2041 ttgcgaatga ggaagctgca ctggggaaac aattcaatgt tggaacgggt aaagcaacca
     2101 cacttttgga attgattcat gcaatcaatg aaattctcgg tgtagaattg acacttgaat
     2161 acaaagaaga gcgttcgggt gatatccatg actctctagc agatattact aagatccagt
     2221 caattggcta tcaaccgaaa tatggtattt taagtggaat gactaattat ttgaaaactg
     2281 aaatcaatta aaacatacta agataagatt agagggtaaa ttcaggctct ttgtcaataa
     2341 ggactgatga gttttgtaaa atcaaatctg ggata
//
LOCUS       DAZKLA010000208         2366 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000208 DAZKLA010000000
VERSION     DAZKLA010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.215"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..611)
                     /locus_tag="ACG85N_002822"
     CDS             complement(243..611)
                     /locus_tag="ACG85N_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFQ0225522.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            761..1243
                     /locus_tag="ACG85N_002823"
     CDS             761..1243
                     /locus_tag="ACG85N_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFQ0225523.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1280..2290
                     /locus_tag="ACG85N_002824"
     CDS             1280..2290
                     /locus_tag="ACG85N_002824"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFQ0225524.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT          834 a          364 c          470 g          698 t
ORIGIN      
        1 attttttcct tattcaaaag ttttgcagga aaattgctga aaaaataatt ttactaagta
       61 gaaaattcaa agccattcaa tcagtgattt ttttcagcac aagaaagact ccttataaaa
      121 atagcagata ctttgtcaat taagtgttta accagggatg ataaaaagga taggattgaa
      181 caaaacctgt ataaggtgat gaatagtttc ctcgccagta aaaggtttat tcgtataaag
      241 ctttatcgcc aaaatttatc atgacctttt ttatcttcaa tattaaatga aatcaattgt
      301 tcaatcaatt cccagttgat tacacttttc caaggaactt tgatgacgtt atccgtatat
      361 acgtagcctg cttcttggat atttttttca agggcacgaa tcgctgcttt ttccggtgcg
      421 atagaaaaat gatttttagc aaaactaaat gcaataataa aggtaccatg gtcagtaaac
      481 ataggttggt tccattttac agttgtgtct agctggggat acgtttctgt tatcttagca
      541 aaaaggtctt tgatcttttg ttgatgttct ttttctttga tttgattgat gatcggctga
      601 aatttttcca ttctaaaacg cctccattaa gtggtttgat ttattttacc agaaaaacaa
      661 tagttatgta taaaaaacga acaaaacaca agtaatgttg taaccgctat atttatatga
      721 taagatgtct caaataataa aatagaaaag agacataaaa atgattgact tggcaaagaa
      781 attcttacac gaaaatatat attttgagtt gtatcatcat cgagccattt atacaaatga
      841 agatgctttg gtaataaaga aagaacaagg atttacaggt acagagacca aaagcttatt
      901 cttaaaagat aaaaaagata ataactacgt atatctgact tttactacga aacaaacgga
      961 tttcaaagca ttagggaaga tcgtcggtaa aaggctttct attgtttcat cagaacgcat
     1021 ggaagaacaa acgggacaaa agcagggcgc tgtttctccg tttggttatg aaaaacagat
     1081 tccgattatt attgatgaag agctcttatc aaatgaaaaa cttgtttttg caccaggtag
     1141 accagatcaa acgatggtca tccaaacggt tgacttaaac aaaatcatcc agctacttga
     1201 gatgccgata tttttgatgc cggcaaacga aaaaaacaat taaccaagta aaatagtgca
     1261 tacttggagg tgaagcaaca tgataagtta tccattatta gaaagaaatg caacgattgg
     1321 ggttactgca ccatcctcag gcgtatcaga ggaacaccat tcgttactcg aatcagcttt
     1381 aaagagaatg gaagaaaaat ctttccaagt agttactgga aagacgcttt ggacgcaaga
     1441 taaggaaaaa tcaggtccag caagaaaacg agcagatgaa ttcaatcaaa tgatgcaaga
     1501 tgaaaatatc caaatgatct ttccgacttg gggaggagaa ttattgattg agattctcga
     1561 ttttgtcgat tttgatgctc tacaaccaaa atggatagta ggttattcag atattagtac
     1621 gttgctactt gctgtcacat taaaaacagg gatagccact gcacatggaa ctaaccttat
     1681 tgatttaaga ggagagtact tggatgaaac gacagcaatg tgggaaagtg tactgaaaac
     1741 aagagaagga gaatcgattc tacagcaatc ttctgaaaag tatcaaaaag tgtggcccca
     1801 tgagcattca tcatcttctg ttttccatct cactgaacca acaaattggc aatcagtcag
     1861 cggagatgca gtaaggatga agggacgatt attaggtggt tgtattgata cgatcaggca
     1921 tttaatcggt actccttatg gagatgtcca atcttttaaa agaaagtata atgatggaaa
     1981 gccgatcttg tggtttctag aaaactgtga gctatcgtca gttgatttaa gaagatcact
     2041 tgttcagatg aaactggcag gctggtttga tggcagctcg gggatcatgt tcggtagaag
     2101 tgcggcagag gatttgttag attataaaat gaaggatgta tacgaagatc tgtcaaagga
     2161 attaaaacta ccgatcttgt atgatattga ttgtggtcat gtaccgccac aactgacatt
     2221 gattaatggt gcctatgcag aagtagcggt agaagataga aaagcgactg ttttgcaaac
     2281 gtttatttaa aaaatttttt cctgccttgg agtctatcaa agaccaataa atctaaatag
     2341 aaaagaaagt gaaagaagct gacaaa
//
LOCUS       DAZKLA010000209         2256 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000209 DAZKLA010000000
VERSION     DAZKLA010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.216"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..782
                     /locus_tag="ACG85N_002825"
     CDS             <1..782
                     /locus_tag="ACG85N_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFQ0225525.1"
                     /translation="QIFYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVK
                     IKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQ
                     HSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNEL
                     KRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFI
                     LSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
     gene            784..1854
                     /locus_tag="ACG85N_002826"
     CDS             784..1854
                     /locus_tag="ACG85N_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HFQ0225526.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            1841..>2256
                     /locus_tag="ACG85N_002827"
     CDS             1841..>2256
                     /locus_tag="ACG85N_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0225527.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYI"
BASE COUNT          779 a          278 c          352 g          847 t
ORIGIN      
        1 tacaaatatt ttatattaca ctttcttcgt ctagaggtgc ccaggtagta atttgttctt
       61 ttttagtatt atttcttttg ttgtttgcca attcaaaatt tagaaagagc attagagaag
      121 tactacctgt aaaaatcaaa tggatggtaa gcatattatt agttctattg gtatattttt
      181 cttttggtac taattatgct aaggattttt tagcaacaat tcctgtatcg attgatagaa
      241 aagtagaagt tatagagtca aatgaatcag ataaaactcc acttccagaa aaagaagtaa
      301 tattacagca ttctagtgat aactccgaaa tttcagctgg tcgttttacg atttggaaag
      361 ctgcgcttaa agttataaga ccaactttgt tatttggtta tggagagacc gatttctatg
      421 ggaatagcga tgcaaaattt ttgaaaaaag tagatcttga tactacagat gtgaatgaac
      481 taaaaagagc acatggaaac atgcataatg gatttcttca agtacttgta tcaagtggta
      541 taattgcttt tatatgtatc tatgctttct atgtgttaaa tatctttagt ttactcaaaa
      601 cattttttag aagtaaagtg aattatgcgt ttcttggtat tgttttaatt tttatactat
      661 ctatttttat agatgaaatg gtggaggctc atgtactttt caataagcgc gatgtcattt
      721 caattgtttt ctggtattat ttgggaagca tttcttactt gtatttaaaa aaggagaatt
      781 aatatgaaga cagctttcat tgcttggact cctttacatt tgattaatat attaaatgtt
      841 tatcaaaatt tttaccgaga atcaaaagga gatttattta tttattctga atttgaaaac
      901 gctagaagat atagtgagcg tataaaagaa ttagggctat ttaatgatgt tgtttttgtg
      961 aatcaccgga aaatcggttc ttcctttaaa cgtaaaatta acttgttaac taaccataat
     1021 caatttttct cggatgattt atccgtttat gatgaaattt ttatacaggg ggaaaattat
     1081 ttcacaaaaa tacttttctc caatgtttac aaaaaaaatc cagatgtaaa actaaattat
     1141 atagaagatg gtttgggtac ttatttgaag aagagtatat tttcaacaag taaaaacgtt
     1201 caaagagttt ttaaaatatt aaataaaaat agcatcttca aaaaaaaaat tgacgattat
     1261 tttgtctatt gtccggaatt gttactttat gatgtaaaaa atataaaaaa aattcccaaa
     1321 atagatgaaa acagtaaatt gatgaaaaac atacaatcaa tctttgggat agaagaagtt
     1381 tttgtcggag ataaatgtat cttttttgat caacctttat tatctgatgg ttatggcata
     1441 gatgaaacag atattttcgc taaagtaaat agtatatttt ctggaaaaaa agaaacttta
     1501 attaagcttc atcctagagc aaggacaaat cgttatggtg aaaaaaacat attgaattcc
     1561 agtttaccgt ttgaagtctt gctacttgaa aatgacttct cttcaacact tttgattagt
     1621 cctatatcta caatttcttt ttcaccatct atgatgtttg aaaagacgat caactctatt
     1681 atgttggcta aattaatttt aaatgaatat acagcattat ctgaagataa aaaagaaaca
     1741 ttgacaaata ttgttctttt ctgtgaacat tataaccgtc tatcagaaga aaaaatttta
     1801 ctcccagaaa actgggcaga attaagaaag ttggtagagt atgggattat ttaacacact
     1861 ttccaagtca aaattagtta agtccagttt ctggtatact atagggaatt tttttattaa
     1921 gggaatttca ttcataacta ttcctatttt tgtaaatatt actactcaag cagaatatgg
     1981 tttgattaat aatttcactt catttgtatc aattttttcg attcttgttg ggctaagcct
     2041 aaattcggca attaataatg caagttttga ctataaagat gacataaatg aatttatgtc
     2101 atcggtactt tctctagcta cactttcgtt aattgtgttc ttattaatag ggaacatata
     2161 ttttttgttt tcaaatgctt tttttgatat gaaccaactt gtatttaatt tgatgttatt
     2221 acaaagtttt gctagttttg taattagtta tatcca
//
LOCUS       DAZKLA010000210         2255 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000210 DAZKLA010000000
VERSION     DAZKLA010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.217"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..625
                     /locus_tag="ACG85N_002828"
     CDS             <1..625
                     /locus_tag="ACG85N_002828"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0225528.1"
                     /translation="INRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYS
                     QSEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQE
                     EWERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVF
                     FYPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            638..>2255
                     /locus_tag="ACG85N_002829"
     CDS             638..>2255
                     /locus_tag="ACG85N_002829"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HFQ0225529.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLA"
BASE COUNT          840 a          270 c          448 g          697 t
ORIGIN      
        1 aataaacaga tttttaggtt tagttaaaac attagaaaaa aatgatgtaa catcgattaa
       61 tcgattaaag cactgggtat ctctaacaaa agaattgtct aatccatctt tgaagaggga
      121 aatagaatat tctcagtcag aggatttatt aaaagcttta aaatggtcgg aagaagtcaa
      181 tcaacagatt agtcaagcaa aaggattaga taaacccttt ttaagtgcta gagatactat
      241 aaaagctctg aaaaaatatg ggaagataat tattgtaagc tcagctaata aggaagctgt
      301 tcaagaagaa tgggaaagac atgaattatt atcattagtt gatgaactgt gttgtcaaga
      361 taaaggaaaa aaagaagata ttattcgaag tgtaattgaa aatggttgtg atttggataa
      421 gatattaatg attggtgatt ctcctggtga tctagaagct gcaaataaaa ataaagtttt
      481 cttttatcct attttggtca ataaagaaaa agaatcatgg gaaaacttaa gaactgatgt
      541 tttatttaaa tgtttatcgg gagattatgt agatattgaa caaaaatata tagatacttt
      601 ttggaaaaat cttactaata agtagaggag aaagaaaatg actataaaag tagatttacg
      661 aaaaaaacca tataatttag atgaagatgc tatttcttgg attgaagaga cgattgattc
      721 tatgactttg gaagaaaaaa taggtcaatt gtttgttaat atgggatcta gtcgtgataa
      781 aaattacttg agtaatatgg tcaatcagta ccatattgga gctgtacgat ataatccagg
      841 aactgctgat gaggtatatg atcaaaacta tatcttacaa acagaaagta aaattcctct
      901 attaattgct gctaatacgg aagcaggtgg taacggagct tgtacagatg gtactgaagt
      961 tggtgtagaa gtaaaaattg gtgcaacaaa tgatgcgaaa tatgcttatg aattagggag
     1021 agtttctggg attgaagcat cggctattgg atgtaactgg agttttgctc ctattgtaga
     1081 tattaataga aattggagaa atcctatcat ttctagtcga tcttttggac aaaaccctga
     1141 taaagtttta gaacttagtt tagcttatat gaaaggtatt caggagagtg gaattttgcc
     1201 tgcagctaaa catttccctg gtgacggtat tgacgaacga gatcagcatc tatcttttag
     1261 tattaatagt ttaggtacgg aagaatggga taattctttt ggaaaagtat actcaggctt
     1321 aatagaagct ggattacctt caattatggc aggtcatatc catatgccag cttattcaaa
     1381 acattttaat cctgatctga aagatgaaga tttacttcct gcaactttat caaaagaatt
     1441 aatcactgac ttacttagag gaaaactcgg atttaatgga ttagtagtta ctgatgctag
     1501 tcatatgtta ggaatgactg gggcaatgaa acgatcagaa ttattgccca cttctattgc
     1561 agctggatgt gatctatttc tattttttaa tgatccagat gaagacttta attatatgat
     1621 ggaaggctat cgtaaaggaa ttatcactga tgatcggtta ttagatgcct taaaaagaat
     1681 tttagggtta aaagctaaat tgggattaca caaaaaagca aaagaacaaa ttttattacc
     1741 taagcaagaa gctctggaaa aaattggttt aattgaaaat aaatcaattt ttaaagaggt
     1801 ggctgaccaa gcaattacat tagttaaaga taaacaagat atttggccta ttgtaccaga
     1861 aaaatataaa aggattttac tagtagatgt acatggggta aaaggtgggt ttggtgaagt
     1921 agttgctgga aatagacctc aagccattga tttattgaaa cagattttgt cagcaaaagg
     1981 atttgaagtt gaaaaataca tttcacctga agaagaattg aaaggattat cgcaagaaga
     2041 aaaaattaaa aatatagctg gaatatatgc tgctaagaga ccaattaaaa atttaactaa
     2101 aaaatatgac ttgatcatta atatagctaa tgttaatcca ggcacagttc aacgaattgt
     2161 ctggccagca agtaaaggaa ctccggatat accgttttac attcatgaaa ttccaacaat
     2221 ttttgtatct gtacagtgtc cgtttcattt ggctg
//
LOCUS       DAZKLA010000211         2206 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000211 DAZKLA010000000
VERSION     DAZKLA010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.218"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..454
                     /locus_tag="ACG85N_002830"
     CDS             <1..454
                     /locus_tag="ACG85N_002830"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0225530.1"
                     /translation="LLVVLVPRIFGLTPYIIEDKEMSPYYAQGNLIYVRPKNTNEILV
                     GDIITYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIP
                     YIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            582..1265
                     /locus_tag="ACG85N_002831"
     CDS             582..1265
                     /locus_tag="ACG85N_002831"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFQ0225531.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1574..1647
                     /locus_tag="ACG85N_002832"
     tRNA            1574..1647
                     /locus_tag="ACG85N_002832"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1608..1610,aa:Arg,seq:tct)
     gene            1806..2165
                     /locus_tag="ACG85N_002833"
     CDS             1806..2165
                     /locus_tag="ACG85N_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFQ0225532.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
BASE COUNT          733 a          345 c          452 g          676 t
ORIGIN      
        1 tcttctcgtc gtcttggttc cccgaatttt tggtttgact ccttatatta ttgaggacaa
       61 agaaatgagt ccatattatg cacaaggaaa tctgatatat gtacgaccga aaaatacaaa
      121 tgagatttta gtcggagata ttattacata ttatgaaaat tcaggaaaaa aaatctcgac
      181 gcgtcgcgta atagccgttg aaaacaacca cgaagatttt tatacaaaag ctgatacaaa
      241 gacttatatt gagcctggag tagtaaaaaa gagaaatatt atcggtagtc ccatctttca
      301 gattccatat attggactag tcctttcaaa aacggcattt gcatggattc aggggttatt
      361 ctttacgatt gctttttgcc taacaggaat cacagcatgg aatacctttg tagagttgaa
      421 aaagaagcgg agacgaacac agaaattttt gtaagaatgt taaaaacata aatgtattcc
      481 agagatccaa cgactcttct gaacatgaaa cacgagaaaa cactcatgca gcttgtagat
      541 ttagtcagtt gttcgtataa tgtgtgaaga ggaggagtag tatgcgcaat gaaatgatgc
      601 atcgtcctgt agtcaaagaa gaactattgg attttatgcg tacccagcaa aaaaaattac
      661 cgggtgagct aggcaaactg gaagaagaag ctcatgtagc agaagtacca gttattcccc
      721 atgaaacagt tgtatttttg aaatttttac ttggacagct tcagcctgaa cgaatcttgg
      781 agatcggagc agctatcggt ttttcatcaa gtgtcatggc cacgacaatc ggagaaaacg
      841 ctcatgttac gacaattgat cgtttcgatg tcatgatcga gaaagctaaa aaaaactatg
      901 aacgtctagg actgacagat aaagtgactt tgcttgaagg tcaagcagca gatatcttgc
      961 cggagttgtc tggtccgtat gactttattt ttatggatag tgcaaaatca aaatatattg
     1021 aatttttacc tgaatgctta cgtttgttaa gaaaaggtgg cgttttgatg gtagatgaca
     1081 tcttccaagg aggaacgatc ttgctcccag atgaagagat tccccgtggc aaacgagcta
     1141 tccatcgtaa attaaatgaa tttcttcgtg ttgtgatgga tcatcccgat ttgacatcga
     1201 caatcttgcc attaggtgat ggtgtgatct taatgacaaa ggaatcagaa acaatcgagt
     1261 tgtgatacat gattatggca gcctgtagat aatcgattga atcgagggct caattcgaac
     1321 ttgatgtaaa agtttctata tttgtaagtc aattaaagga tacaggaaat ctaaataaac
     1381 aggagtagca tgaagtcaat gtagctcctg ttttcaattg ataaccttag aacagctgaa
     1441 ggagaatgtc tcgttttaaa agattgattt agtagttggg ccaaaataat tcattatttg
     1501 ctaattaaat aactatttcg tattgacaga agcaatttaa ttatctataa ttattgatgt
     1561 tccgctgatt cttgtcatag tagctcagct ggatagagca tccgccttct aagcggacgg
     1621 tcgggggttc gaatccctcc tgtgacgcta aaaaaccgtt agaaactgag gtttctaacg
     1681 gtttttcttt gttttttgtg ccaatattgt ttgttttcaa aggcaaataa aataaaaaaa
     1741 catccttatc gagtacaatt ataaggagca aaaaaataaa actcatcaga aaagaggaag
     1801 aagatatgac gatactatct agaattttag tgactctagt tgcaattgaa tttttttata
     1861 tcatgtatat cgaaacggtg agaactgatt ctgacacgac aagtagggtt tttaaaatga
     1921 gtaaagaaga gttaagcaga aaatctgttc aaacattgtt taagaaccaa ggagtgtaca
     1981 atggactatt aggagtggga ctactttatg gagcttatct ttcttccgca tctaaagaaa
     2041 tcacaagtat gctattaata tctatttttt ttgttgcatt atatggcagt ttgactagcg
     2101 atcgttggat tttactcaaa caaggtgggc tagctctgtt ggcattgctt tctattttct
     2161 tataaatata gaaaaaataa gaatttatct aacaggaaac tttgtg
//
LOCUS       DAZKLA010000212         2155 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000212 DAZKLA010000000
VERSION     DAZKLA010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.219"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..536
                     /locus_tag="ACG85N_002834"
     CDS             3..536
                     /locus_tag="ACG85N_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225533.1"
                     /translation="MSFFKEYLEIINFRLKTKKESVIILVYFQGGNLMIKKLFLLSIL
                     TLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCS
                     NRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAF
                     SFAREDEDNPKTYDILA"
     gene            complement(597..962)
                     /locus_tag="ACG85N_002835"
     CDS             complement(597..962)
                     /locus_tag="ACG85N_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFQ0225534.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1038..1283)
                     /locus_tag="ACG85N_002836"
     CDS             complement(1038..1283)
                     /locus_tag="ACG85N_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0225535.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1353..1607)
                     /locus_tag="ACG85N_002837"
     CDS             complement(1353..1607)
                     /locus_tag="ACG85N_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225536.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1771..2094)
                     /locus_tag="ACG85N_002838"
     CDS             complement(1771..2094)
                     /locus_tag="ACG85N_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225537.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          810 a          363 c          374 g          608 t
ORIGIN      
        1 ttataagctt ttttaaagaa tatttagaaa ttattaattt tcgtctgaaa acaaaaaaag
       61 aaagcgttat tattttagtc tattttcagg gggggaacct tatgataaaa aaactatttt
      121 tactatcaat attaacgcta gcaatggcag gttgttctga ctctggctta gcaactgaac
      181 tgatcacaaa atcaactgaa gccacgacag aggagttaaa atgtggaaac gatcacagtg
      241 actacagagg cttaaatcga cgatgcatgg gaatattagg cagaagcaat tgtaagcatc
      301 gatgtagtaa tcgaagaagg tgtagaagaa gcaacagaaa aatcaaaaga aagatcaaaa
      361 gaagcaatct gtgctgtgca tcgtatacag acgtcgaaga tgcggtactt ttaaaccacg
      421 tcaggagatg ttcatttatg tctagggaag ttggcgatga accaaaacta gagtacgctt
      481 ttagttttgc acgtgaggat gaagataacc ctaaaactta cgacatttta gcttgatcta
      541 agatggttaa agctttttaa tgaagtaaga ggagatagaa ggcttttcta gttatattag
      601 gtaagataaa ttcctttttg ctccaaattt gctaaacatt ctgctagata aacttcatcg
      661 tgttccggat aaatcggcac gttgcgctct acagtaggta aatcaaaaaa tgcttcgcat
      721 gatttgccac gatacatagg atttttccac tctggtgctg ggctgtaacc acgtttttcc
      781 atttcatcca tgacaagctg atgatattgg aataatctat aaggagaata gtgaaaaaca
      841 taatccacag tagcatgttt ctttccccag ccatttcctc ttaatgcaca gcattcccga
      901 tgttgtccta aaagctgttg acgaggaagc tttgaaatca atttttcatg ccataatcgc
      961 atagaaatgt ctcctttata tatagattgt taaccttaga cgttaaatga aaaaaccagc
     1021 accaaggctg gaaaaaatca cttcagtttg cttgctgttt ctgagcgacc aattaggtaa
     1081 tcaacggaaa tatcgtaaaa gtcagcgata tagattaatt caatgatgct tggaatttct
     1141 tctccttctt cgtacttacg atatttgaat aaaggaatac ttaacgcgct agaaacagct
     1201 acttgagata aagatcggcc agttcttaag aaattcagac gacaacgtaa agtatccagt
     1261 aaaatagctt cttcgatact catttaatca cctttcatta tagtagaata gttataatga
     1321 ctatagaaag ctacacatgc aatcaatagt actcaatgtt ttttatttaa acgtatcaaa
     1381 taaagaatga cgatcaataa agacaaaacc agtcctgata attcttctat aaaaacacta
     1441 tcgggttcaa agtagttgta attaaaaact ttccacataa agtagatagc cggaagagtc
     1501 aaaatagcta aataaaaccg ttctttgttc caattcataa gataaacaat acttgcgacc
     1561 agtgcgaccg caggcaccca aaacaaaact ctcaccaacg taatcatgta acggactcct
     1621 caacgattca tataataaaa gataacatgt tattgcatac atcacaaatg aaatgctagg
     1681 aaaataaaag aagaatagat caatgaaaaa tgaaaaagca gaacagtttt ctgctgataa
     1741 gcaggaactg ttccgctgaa tgtgtcttat ctatcgagaa aaaatcaatg gattgatatc
     1801 taagtaattc aatacatata aaaagaggac gatcgcattc actaaaaagg aaagaatgat
     1861 gatcccatag cgaatggaaa catatttaac aaatactttt gtttgaatac gggcatatct
     1921 ttttccttta tagctgagca tcatgacaag aagcaaaagg gtaatcacac cttgaaaaat
     1981 cattccaata aacagaattc ctgaggtttg ataatttaat tggagaaaat aagctgctgc
     2041 cgcaactaaa tcgaataaaa tcgcaataaa tggcaaaata atcactccta acattgagaa
     2101 taaataaaag aataacattt tttctaaaat aagcaaataa aaattaaagt aacaa
//
LOCUS       DAZKLA010000213         2003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000213 DAZKLA010000000
VERSION     DAZKLA010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.221"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACG85N_002839"
     CDS             <1..378
                     /locus_tag="ACG85N_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0225538.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACG85N_002840"
     CDS             426..1826
                     /locus_tag="ACG85N_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0225539.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAZKLA010000214         1979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000214 DAZKLA010000000
VERSION     DAZKLA010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.222"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(346..528)
                     /locus_tag="ACG85N_002841"
                     /pseudo
     CDS             complement(346..528)
                     /locus_tag="ACG85N_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(728..1744)
                     /locus_tag="ACG85N_002842"
     CDS             complement(728..1744)
                     /locus_tag="ACG85N_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0225540.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          556 a          460 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DAZKLA010000215         1960 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000215 DAZKLA010000000
VERSION     DAZKLA010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.223"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            818..1291
                     /locus_tag="ACG85N_002843"
     CDS             818..1291
                     /locus_tag="ACG85N_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFQ0225541.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1300..1527
                     /locus_tag="ACG85N_002844"
     CDS             1300..1527
                     /locus_tag="ACG85N_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0225542.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          633 a          324 c          298 g          705 t
ORIGIN      
        1 tcctatgatg aaaagattgt actattcatt gatcataacg attggctatt tgattgtatc
       61 aaatctagga aatatggtat ttggtatcag taaagaattc agttggacta ctacattatg
      121 ggaatctcta ttctttttca tcttcgtatt tttgttacaa aattatcgaa aaaaataaac
      181 atgtgccatc tatctgttgg cacatgtttt aggatttatg gtacttttta ttcggcaatc
      241 cattttattt ttctacttct tcttaatacc tctaacactt ttgatttata aactaatcca
      301 atgcatctac ttgcgcttat taagctattt cagctagata gtaccacccc atgcgtcaat
      361 gcttgatttt ccttttgtat aacattgtat aagcaataaa ggacaaataa gtggtgatta
      421 caccaaacca ttgaataaga aaaattctga tacgtaaata aacatgaact tgaaaaaatt
      481 aatgaatcgg ttagctacta cgtagttgtc ttttttctca tacagttctg ttaacttaca
      541 cttctcttcg ctagataata aaaaatcctc caactgtatt gtccaatata atcccaggca
      601 tctaaactag cattatagaa gagacacaca tagaatctaa aataactggt tttaaatgtt
      661 tcttacaatc aaaaaaagct gttaaatgca ctccacacct tcatactcta tcatttttta
      721 taaacgatat atttttattg tttttcgata aaaaatagtt gttttacaat aattattctg
      781 ctatatttta gctatcaaag ataaaagagg gataaaaatg aaatttaaaa cattgctatt
      841 aaaatttgtc gttttaggta taagtgccgt atttattttg ttcggcgtac ttttttatac
      901 tcagatcgct acaagcgaaa aaggattcaa ttttgattgg cagaccacat tatttgtttc
      961 tgtcatttac tttgcccttc tcctagggat cgttattgct tatttcttat tgaaactatt
     1021 gaaaaaaatc gaagataacc aagctttttc cactcaaagc ttaaacatac taaaaaaaat
     1081 cgaacgaacc attttgttta ttggtttcgc cttttttggc ttacttccta aatttatgaa
     1141 agtagcggat ttagacgatg ctccaggttt gatgttggta ggaatcgctg tcgtattcat
     1201 tccttttgca gtatacacat taacaagcgt gctggaaaaa ctgcttgaac atgcgatttt
     1261 acttaagaca gatcatgatt tgacagtgtg aggtgcgaca tggcaattat cgttaattta
     1321 gatgtagtat tagctcaaaa aaaaatgagt gtgactgaat tgagtgagaa agtgggcatt
     1381 acgatggcaa acatctctat tctaaaaaat ggaaaagcaa aagccatccg tttttccact
     1441 ctcgaaaaaa tctgccaagt tttggagtgt caaccaggtg atttgttgac ctataaaaat
     1501 gactcgaata ctataccaga agaataaaat aaagtagaac aaacagtaaa taaaaaataa
     1561 tctgaatgat agatgtgtga gacttttaga agttatttac gtctattatt cagatttttt
     1621 atttcgattg aataccgttt tatctataaa taagatatgt actgactcat acaaaggggg
     1681 ccttttcttc tccatgtact atcacttttc ctgatacaac tggtttcctg ttacgttgta
     1741 gttggatctg cttttagtat aatactttat tcattccacc agactgatag cagtttgagc
     1801 agtctaaata atcatgtagt attacaggcc attctgtttc ataccttgaa gtgcttaaaa
     1861 cttcagctaa tggcccattg tgttaactct tactccattc ttttttagta agctcccata
     1921 tatactgtaa agccttctat atattatgtc atgttgcttt
//
LOCUS       DAZKLA010000216         1949 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000216 DAZKLA010000000
VERSION     DAZKLA010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.224"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..260
                     /locus_tag="ACG85N_002845"
     CDS             <1..260
                     /locus_tag="ACG85N_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225543.1"
                     /translation="LKRKVTDRNKNLLNVVMICYISFLILGLPQAVFPEMFSGDWGIV
                     SYSILTLIAMVLGTSFYNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="ACG85N_002846"
                     /pseudo
     CDS             467..667
                     /locus_tag="ACG85N_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..>1949)
                     /locus_tag="ACG85N_002847"
     CDS             complement(1379..>1949)
                     /locus_tag="ACG85N_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0225544.1"
                     /translation="DQNLDRQLAMMEDLNIDKLFQEKASGKDIQRPEFQKLLNYIREG
                     DCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFL
                     SLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQKRLVFKTVVDML
                     NQGVPVAEIAKENGLSRPTIYKIKKANDL"
BASE COUNT          630 a          333 c          352 g          634 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatct
//
LOCUS       DAZKLA010000217         1920 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000217 DAZKLA010000000
VERSION     DAZKLA010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.225"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1149
                     /locus_tag="ACG85N_002848"
                     /pseudo
     CDS             5..1149
                     /locus_tag="ACG85N_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1320..1778
                     /locus_tag="ACG85N_002849"
     CDS             1320..1778
                     /locus_tag="ACG85N_002849"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFQ0225545.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
BASE COUNT          629 a          361 c          378 g          552 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccaa aagatctagc cgatcagatc
      661 ggattttacc aaaatgctat cggagccgta cttggtcccc aagatagctg gttacttcag
      721 cgcggaatca agacactaag tgttcgtatg gaggaacatc aaaaaaatgc ttttgtggta
      781 gctgattttc tattttctca tccagcagta gaaaaggtct actatccagg attacccgat
      841 catgaactcc atggagttgc taagcagcag atgagcggct ttagcggaat gatttctttt
      901 actttaaaaa atgaagaatc tgctattcca tttgttgaat cactccaact atttacttta
      961 gcagaaagtt taggtggcgt agaaagttta gtggaaattc cttctgtcat gactcatgcg
     1021 tctattccaa aagaaaaacg agaagaggct ggcataaagg acggcttgat ccgtctttct
     1081 gtaggaattg agtatggtca ggatctcata aacgatttag ctcaggcatt tgacagaatc
     1141 aagaactaga agtcccaata aacgacttag cagaaaaatc taaaaaatat ttcagattgc
     1201 tacaccttgg aaacgataag tgttagaatc aagtagaaag acgtttttgt aaatttcact
     1261 aaataattct ttagtgaaaa gcatcaaaac gaatgaacaa acagatagga gtcttacaaa
     1321 tgagaatgaa tgtcgaaagt tttaatcttg accatacaaa agtcaaagca ccttatgtac
     1381 gtgtagctga tcgaaaaacg ggtgcgcatg gcgatgtcat cattaaatac gacgtccgct
     1441 ttaaacaacc aaataaagaa cacatggaca tgccaagtct acattcactt gagcacttga
     1501 ctgccgaact aatccgcaat cacgcagatt acatcgtaga ctggtctcca atgggatgcc
     1561 aaacaggctt ttacttgact gtattgaatc atgaaagcta cgaagacatt ttagatgttc
     1621 ttgaagcaac aatgaaagat gttcttgaag ctactgaagt acctgcaagc aatgaaacac
     1681 aatgtggctg ggctgccagc catacactag aaggtgcaca agctttggct cgtgaatttt
     1741 tagcaaaacg cgatgaatgg tctgaagtag aagcatagtt tctaaaagaa cgtaaagatg
     1801 agaagcaata tagctatctt atcttcgtat tttattaaat atttattgtc tgaaaagagt
     1861 tctaaacggg aaaacaaata gctagttttc agtttagcgc tctttttatt atataaaata
//
LOCUS       DAZKLA010000218         1919 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000218 DAZKLA010000000
VERSION     DAZKLA010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.227"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1340
                     /locus_tag="ACG85N_002850"
     CDS             <1..1340
                     /locus_tag="ACG85N_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225546.1"
                     /translation="KIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYA
                     YGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVV
                     VNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDIT
                     TYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQ
                     FPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQ
                     SKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAA
                     DNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEI
                     TTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1327..>1919
                     /locus_tag="ACG85N_002851"
     CDS             1327..>1919
                     /locus_tag="ACG85N_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFQ0225547.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTA"
BASE COUNT          648 a          291 c          331 g          649 t
ORIGIN      
        1 aaaagataga tgctgctttt ttatttagtt gtttatatac ttttgcaact tatcgaacag
       61 ttgatatcta ttatcgatcc gcaattgcag aagcaatcgc tcttacgatt atcccagctg
      121 ttttatatta tgcttatgga ttgatattca atagagaaaa aaaagtcatc gctttatctg
      181 tcagtataag tctacttgtg tatactcatg tcttaactac actattgtgt agtgcattga
      241 ttgcttgtat gattatagtt cgtttgtttt taagcaagca aataaaaaac gatataaaat
      301 ttgtggtcgt gaatttagta aaggctgttt ttttgacttg tgcattaact agctattttt
      361 ggtatcctat gttgcagcag atgaattatc aagaaataca cagtcccttc aaaacagatt
      421 tgcagctaga agcactttct atttcagata gtatcattca agcgttaaat aacgatatta
      481 caacatacac tatggggtta ttaggattgg ttgcgttgat ttttccttta ttcatactga
      541 aagaaatgga gaaaaaagaa aaaatcatct atgttggcgt agtttctaca tgggtatgcg
      601 taactaattt atttccatgg tttttgtttc aaaaaacacc gataagctta ctccagtttc
      661 cttggcgtat cttaggtatc caaatctttt tttcttcaat gatcataacg cttgttttta
      721 tgaaaaagac tatgaataaa aaatattcat ggttaatcat tggcgctact gtggtgtttc
      781 tactcgtcac gtctatagca gcaaaagaaa attacgcaaa agttatccaa tcaaaacacg
      841 gtcatattgt tatcaatgaa gaaacactcc cacgttatac aacatcagat atgggaggat
      901 tatacgatta cgccccaaca gaagcgataa aagaaaggcc tcacttagaa aaacatgaag
      961 taaaagtagg agaatcgtgg gaaaaaggga agtataaagc tgcagataat aacattactt
     1021 acacagtggc aagcaacaaa aagcaaaaag tgacagtacc agtatatgct tatttaggta
     1081 cacaggtaaa agtaaacggt aaagtcgtaa atgactctta taaaaaacat ggcttagtga
     1141 atgtggatgt aaatgctgga gaaaataaga tagaaattac aacaaaatat actccaactg
     1201 ctctcatggc tttatggatc agtattctga cttattgttt agtggtgtat tcaagtttta
     1261 aacgatttgc acctaataag ttgaaagatt ctcgatatgt gactaaaaag gagataaaca
     1321 agaagaatga aaataaataa aaaagtagta atattgagtg gattaattat agttagtagt
     1381 atatattctt tcatcttttt caaaaataca cagagtattt ataccactgg agatctttct
     1441 tttcacttat ctcgaatcaa ggggttatcg actattttta ccagtccgat taattatgag
     1501 acattcaatt atactggata tggtgtaaat tatttttatc cctttctgac tttttttcca
     1561 gctgtaatgt tatattggat gacaaaaaat ttgattgtaa gctatattat ttacgtttgg
     1621 ttattgaact tgtgtacaac actcgtagct tatcattatg gcgagcggtt tttaaaacaa
     1681 aaaaaagcgg catttttatt tagttgtctc tatattttct ctgcttatcg gacagtagac
     1741 atctactatc gttccgcaat tgccgaagct atcgcaataa cacttgttat ccctgtatta
     1801 ttttatgctt atcaaatcat ctctggaaaa gaagaaaagt atccgtctgt aaagctagca
     1861 ttgagtatga gtttgctagt gtacagccat gtactatcaa cattaatgag tacagcatt
//
LOCUS       DAZKLA010000219         1887 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000219 DAZKLA010000000
VERSION     DAZKLA010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.228"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(331..786)
                     /gene="tnpA"
                     /locus_tag="ACG85N_002852"
     CDS             complement(331..786)
                     /gene="tnpA"
                     /locus_tag="ACG85N_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFQ0225548.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1029..1887
                     /locus_tag="ACG85N_002853"
                     /pseudo
     CDS             1029..1887
                     /locus_tag="ACG85N_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
BASE COUNT          599 a          366 c          355 g          567 t
ORIGIN      
        1 gtaactcaaa aatacttcca aaggtgttcg ataatccagt gattttctag gaagattatt
       61 tctgcgccag cattttctaa tgtttgaatt tgtgttttgt aatcttggtt gcttgtgcta
      121 attcttgaat agccgtaaat tgccaaaatt tcatcaccct ttctggacat aagtttctga
      181 caccagttaa aatcaagtct atcagtaacc catacacccg tgaggacggg tgtattttta
      241 atccccctag aaggggtcga cactcgctgc cccttaaaag gggattttta aaagaccgcc
      301 aactgctcat cttcactcac ttacttctct ttatttttta aatggttcta cgtactctct
      361 tttacttata atatatcgaa tgcgatcatc agcttcttgt tcacggatgt atttttcatt
      421 tgtcttttga ttcaatccga ctgtactcac atagtatctc ttcgaccaaa aggatttgtt
      481 tccgtattta tatttgagat ttgcgtgctg ctcgtggatt agtaccgcac tacgaccttt
      541 cagatacccc ataaaactgg atatacatat ttttggtggt actcgaacaa gcatgtgaat
      601 atgatctagc attgcatgtg cttctatgat ttccacatct ttcatttcgc acaacttcct
      661 aagtattttt ccaagatctt ttcgtaattg cccatagatt acttttcgtt gatattttgg
      721 tgtaaaaata atatgatact tacaattcca acgagtatat gctaaacttt tatcgtcatt
      781 tgacatgaaa aaactccttt cgatctgatg agcggttggt agccactcac tagtatatcg
      841 aaaggagttt ttatcgctaa agatacttct tccacgcgta gaaacgcgcg gttttcttgt
      901 ttcgcacgct ttacgcacaa aactgactga agtcattaag aaaataaaag ggtctaaaac
      961 ttttaagttt tgtaccgacc cccaaaagtt agaccaaaaa atctaacttt tggaggtcgg
     1021 tattttttat ggctaagtat acttttgaac ttaaattaaa gatcgttcat gattacttgg
     1081 atggtaaagg cggaagtgat tatctggcaa agaaatattc cattaaggct ccatcacaag
     1141 tgaagcgctg gatcaatgcc tatcaagaat ttggtgaaga gggtctagta agaaagcgac
     1201 aaaagaaaaa atattctgtt caatttaagc tggatgcgat agagttatat ctaacaagtg
     1261 agctgtccta tcgtgaagtt gccaacacac ttaacatgaa caaccctaat ttgatcgcta
     1321 gttggatgcg tcaattccgt gagggtggga tcgatggact ctcaaaaacg aaaggatgtc
     1381 cacctatctt gtctaagaaa aatgaaccca aaaataagaa aaaatctaca actaaagcaa
     1441 cgtctaaaga acgtgaacgc atcgaggagt tggagaaacg agtccgttcc cttcaaattg
     1501 aaaatgcctt tttaaaagaa ttgaggaagc tgcgaaaaca ggaagcccaa caacgacgaa
     1561 tgaagcaatc gcacgaatca tcgcaagcct ccgaggaccg tttaaattag ttgagcttct
     1621 agaaacttta aagttcccta aggcaatctt tatgtactgg caaaaacgat tggaccgtaa
     1681 aaatccagat caagagattg aagcggaaat gcttaaagtt cgggagaagc acaaggatta
     1741 tggctgttta cggatgacca atgaactaag aaaccgcggt ttctctatca ataaaaagaa
     1801 agttcagcgc ctgatcaaga agctaggaat aaaagtaacg acatacacac gtaaatcacg
     1861 gcgctacaat tcttatcgag gacaagt
//
LOCUS       DAZKLA010000220         1837 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000220 DAZKLA010000000
VERSION     DAZKLA010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.229"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            199..396
                     /locus_tag="ACG85N_002854"
     CDS             199..396
                     /locus_tag="ACG85N_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225549.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            502..984
                     /locus_tag="ACG85N_002855"
     CDS             502..984
                     /locus_tag="ACG85N_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225550.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1042..1638)
                     /locus_tag="ACG85N_002856"
     CDS             complement(1042..1638)
                     /locus_tag="ACG85N_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0225551.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT          569 a          275 c          367 g          626 t
ORIGIN      
        1 tttttaagga atatgtttta attttttgaa aaaaattctt ctaaatacaa cagctgtttt
       61 aattgtgaaa acgaaaacag tacgctataa tgcctgtaag gttgattttt aactgtaaat
      121 aaaaatcaat gagttaaatc tccaaagaaa tagcagtggt aatgtggaga aaagttccag
      181 cagtttagga ggattttgat gacggaagaa atgattaatt taggagaaca atatgcatgc
      241 aaaccaattg gttttacaaa aacggtcatt ggtgaagttg tatcaaaaat gacaaactgc
      301 gccgtggtca aagttgccca atgtgctgca gaggaccaag aattattaga tgaaaaagca
      361 agcatggttg ttgcaaaata tgatacattt gaataaagaa aacaattgga cgtgcggcgt
      421 gctgcaagtc caatttcttt gagaaggtaa atcagtattc ttttcctatt ttctggagaa
      481 gaaaaagcga ggcgagttgt catggtaaca ttctttacgg ttctattgtt tttattcgtt
      541 gttgtgatgt ttttatttct ttggctggtt cgaaaagaaa ttatttatcg gactgtgcga
      601 aatcgatggg tctacttggt gatacccttc ttagtgtcac tagtcatttg gtatacactc
      661 atcagtcatc ctacagcaga tgaattggca aaagggatct tgtcagcaat gatttttatt
      721 agttttttgt tggacagtag aggaatcaca gaagaaggac ttgttttgaa tagctttgac
      781 aaaaaaggtg ttcctttttc tgagatcaat aaaattgtat tatatcagcc aaaaggttct
      841 aaaatcgtaa aaatgaattt tttccgaaac ggatggcgag gaccgatgct gaaattttct
      901 gcatcattag aagaattagt tccatttttg tcccaacgct taaacgatga ggcagagatt
      961 gatattatga ttgatccaga ataaaaacag aggttgcgac aagatgtttg tcacaacctc
     1021 tgttttttat ttcaaataaa attatccaaa tacgcggaag cgatcggcat cgttcatgta
     1081 ttctttttcc tcagccggtg tttctggtga accgaatact aattgcgaac gtagtttcca
     1141 gtttgaaggg atattccatt cttttgcaac tgcttcgtca ataactgggt tgtaatgttg
     1201 caagtttgcg cccaatcctt tatcaactaa tgctacccaa gtattagctg ttgcgattcc
     1261 gtttgattgt tctgaccaat ctgggaagtt gtctgcatat aattcaaatt gttcttgtag
     1321 accttttacc acgtcagtat ctttgaagaa taatactgta ccataaccat ttttgaaacc
     1381 agctaatttg ttttgtgtat ttgggaaagc ttctgctgga gtcaatgggc gaagtgcttc
     1441 ttcagtgatt tcccaaagtt tttcatgtgc atctccgaaa aggataactg cgcgtgttga
     1501 ttgagcattg aaagcagttg ggctttcttt aattgcttct ttgatcaaag ttgttaattc
     1561 ttcattagaa agtgaaacat tacgtcctag atgatagatt gaacggcggt ttttcaaagt
     1621 gtcagtaaaa gttgtcataa taaaatttct cctttgattt gcgttttgca tttttttaat
     1681 tgattacgaa atgaagtata gcaccttaca aaaagtaagt aaacagatat gctcacaaaa
     1741 aataagcgaa gttcaaaaaa caattttaat aagaaaaatc gttatgtaag gattttctta
     1801 gttatcgctt tcattgttgt gttttaggaa tttttgc
//
LOCUS       DAZKLA010000221         1822 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000221 DAZKLA010000000
VERSION     DAZKLA010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.230"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(245..466)
                     /locus_tag="ACG85N_002857"
     CDS             complement(245..466)
                     /locus_tag="ACG85N_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225552.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(564..809)
                     /locus_tag="ACG85N_002858"
     CDS             complement(564..809)
                     /locus_tag="ACG85N_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225553.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1049..1663
                     /locus_tag="ACG85N_002859"
                     /pseudo
     CDS             1049..1663
                     /locus_tag="ACG85N_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          608 a          320 c          270 g          624 t
ORIGIN      
        1 ttactatata cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag
       61 ttcattgggt ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt
      121 tatgtcctat ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt
      181 gtaatatgaa gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga
      241 accattatag atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa
      301 atgtttcaaa ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt
      361 aagtccaatc aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa
      421 ctgaagccaa ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc
      481 aagacgctac ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta
      541 tagcaaacaa atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg
      601 cttctattct taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac
      661 tcatttattg ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg
      721 gcatcctgat agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga
      781 acttctttta caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat
      841 ctataatatt tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta
      901 ttataaacta tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa
      961 atagacaaaa atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat
     1021 aaacgttata gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat
     1081 cgatcaaaat gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt
     1141 tatcgaaaaa caatctggag cgacgattag tcatcggccg attttccagg aagctttaga
     1201 ttttgtcaga gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta
     1261 tgacgagatt atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac
     1321 cagtttaccg atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga
     1381 cttaatcatt taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg
     1441 ccaggcacaa ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt
     1501 atacagtgca gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga
     1561 tcttgagaat ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac
     1621 aatttaccga ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac
     1681 gtaattatta attagctttg tcatcaatac agacaatcat tttagattca tctagctaag
     1741 ttgattacat ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg
     1801 tccttattta aaggcattta ac
//
LOCUS       DAZKLA010000222         1822 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000222 DAZKLA010000000
VERSION     DAZKLA010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.231"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..1745
                     /locus_tag="ACG85N_002860"
     CDS             132..1745
                     /locus_tag="ACG85N_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0225554.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          576 a          332 c          378 g          536 t
ORIGIN      
        1 tttaggtttt atccacaaat tatttctcta tattttctgt ctattcaaaa taaacaataa
       61 ttttccggca caattatttg ctgtttttta ccataacata gtacgatcaa cgtagaaaag
      121 gagatgtttt tatgaaagtt gttgtcattg gcggtgtagc tggcggtcct tcatttgcca
      181 ctcgtttccg tcgattgaat gaagcgcacg aaatcattat ctatgaacgc ggagaaaata
      241 tttcttatgc aagttgtgct ttgccttatt atttaggagg tgtgatcact gaccgtgact
      301 cgctgatcga acgtacacca gaaatattga aaacaaaaaa caacatcgat gtctttacta
      361 aacacgaagt aacagcaatc gatccttcta ctaagcgatt aacagttaaa gacctatcca
      421 caaatgaaga aataaaaaca gattacgata agttgatcat ctcttctggt gctagaccag
      481 attatccgga tattcccgga gtttttgaag cagaaaacgg ctttgtcctc cgtagtgtga
      541 cggatgcgga tcgaatcaaa tcgttccttg aagaaaaaaa tccacaacat gtcgtcattc
      601 ttggtgcagg tgttatgggt ctggaattag ctgagaattt caagcatcgc ggcttagacg
      661 tgactttaat cgatcaattg ccacaagtcg ctttccctta tgatccagaa attgctaatt
      721 tagtttatga caaattgctg aaagaaggat tagccgttca tttagaaaca agagttactg
      781 agatccgtga taaaggtcga gaaatagtat tatcagatgg ctccgtcctt tctgctgata
      841 tgctaatttt tgctgttggt gtttctccga agaatgaagt ggtgaaagca accggcatac
      901 aattatcaga tacaggtcag atcattgttg atgaccagtt acaaaccaat ctgccggaca
      961 tctatgcgat tggcgatatt atcgaaacga ctagtgtagt gactggtcag ccgatccaaa
     1021 gtatgctttc cagtgcggcc aatcgtcaag gacatatgct ggcagatatt ttaaatggta
     1081 cgcctatgcg ctatcgcgga tatattggtg caggtgtcgc aaaaatcttt gatcatacag
     1141 caagttatgc gggaatgaca gaacatgaat taaaagcatc aggcataaca aattataaaa
     1201 ctgtttttat cactcccttt gaccatgcct atttctatcc agaagctaca agattaaatc
     1261 taaagctgat ttttgatgca gatagcggtc gtattttagg tggacaagca tttggagaaa
     1321 aaggtgtcga taaacggatg ggagaacttt ctgtagcaat caccggaaac ttgacagtct
     1381 ttgatttgcc tgatttggag ttgccttact ctccaccgta ttctactacc cgtgacccgt
     1441 tgaatatagc tggttatgtc gcaatcaatc aaatgacgaa tatcgtagaa acgatcaaag
     1501 caagtgatat acccgaaaac gatttgaaag aagcgttctt tttagatata cgtgaaccta
     1561 ataaagcacc ttctggcagt atctcggcaa caaaaaatat tccaatgaat gagttacgag
     1621 atcgaatcaa tgaaatccca aaagataaaa aagtttacat tactttcaga agaggattga
     1681 atacttatac ttctgcccga atcttggcag gtttgggtat caaagcgatt ttgattgaag
     1741 aataaaaaaa cgaccgcggt gggtagccgc ggtctaggag ttatttttac ttgaggagta
     1801 aaaatgaaaa agtatatagg gt
//
LOCUS       DAZKLA010000223         1781 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000223 DAZKLA010000000
VERSION     DAZKLA010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.232"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(227..1693)
                     /locus_tag="ACG85N_002861"
     CDS             complement(227..1693)
                     /locus_tag="ACG85N_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0225555.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT          565 a          359 c          312 g          545 t
ORIGIN      
        1 aaaaacaagg gactgtgaca aaagtcgtgt caaagtccct tgtttcgaat aaatggtgtt
       61 caatcagctg accttcagca ttagctcaaa aaacggcaaa atatgaggag ctatttttgt
      121 ttggctgttc ttattacttg atgcaggaca gctttttcac aacctctgat tcacggtgtt
      181 ccagaagtag cttctgtcac aacctcttgt aacaagtatt tctattttaa ggctgaacca
      241 ctgtttctgg ttcagtaatt tctttgtttt tgtctcgacg agcaattgct ggcaagatca
      301 tacctaataa gatcaagaca attggtgtga tgatattcgt gaataattgg aaccaccatg
      361 ctttaggatc agcagtataa tctacttttg gcaccattcc taggatacat gcaaacgcag
      421 tgaaggcaaa gcaccaagca ccaaagataa aaccgatttt tggattttta acaaatttat
      481 attcagaatt gaatttctta taagctttat tcaacatcat atatgccaag aacacccata
      541 ggtaacgcat tggcatgaca actgagttca agttcgtcaa ccattttact aattcattca
      601 tatctttgat accaaagatc ggcaacaaaa taatgattcc tactagtata cctgtcagaa
      661 gataaccatt caccaatgta cctttacgat tacgtttacg tagccagttt ggtacaaatt
      721 ctggatccgc atctgctaac aagattttta gtggtgcatc aattgaaaag gctaatgcgg
      781 agatttgtcc aatcatgttt gtcaatgcgt aaatgatcat caatgagtta ccgataccat
      841 agtaattacc taaaatttga aaagcactgt atgccccatt tgccatcaag tctttaggga
      901 tattatcgct agcaaacagc atacccattg cgactgagcc taatacagca caaatcccta
      961 ccattgctgc taagaagatc atccctttag gaaattcctt tgcaggattt cttgtagagt
     1021 tgacataagg ggatattttt tccgcccctc caactgcaaa tactagcatc gaaacggttg
     1081 tgaaataact gaaatcaaat gttggaatat atgttttcac ttgattcata tgtggtgtgg
     1141 caaattccat cgttggattg atgaacggtg ccgttacagc taaaagaata aataagatcg
     1201 acatgacgaa catcgctgtt ccggccagtc cgccaatcac tttcaaagta gaaagtcctt
     1261 ttgttgataa atataaaaag gcaagaaata taataagtga aataactgct accatttgta
     1321 ctgacatcgt atttacgata tttccatttc cttggactgc ccaaccacca gcaatcagaa
     1381 ttgcttgagg cttttgtgcc aaataaggaa tatggacgac ccagtaagtc caagcggcat
     1441 agtatgccag tcgtttatta gatgtcgtat tttcgaccca tgaactgacg cctcctttac
     1501 tatccttgaa ggctgatcct aattgtccaa cgatcaatgc gtatggaata aaataaatgg
     1561 ccaaaatcaa tagccaagag gtgacaactg atactccttg ctgagcatag ttattaacta
     1621 cattccccat gccccaaacc atgttaaatg caattaacgc aacagtaaac cagcgtaatt
     1681 gtttgttgtt cattcgttct ctcctttatg ataaaaattt gttatccaga tgtaatcata
     1741 ttcaattttt aacaaactta caagacagtg ttattttatg t
//
LOCUS       DAZKLA010000224         1767 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000224 DAZKLA010000000
VERSION     DAZKLA010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.233"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..938)
                     /locus_tag="ACG85N_002862"
     CDS             complement(<1..938)
                     /locus_tag="ACG85N_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFQ0225556.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIME"
     gene            complement(1097..1363)
                     /locus_tag="ACG85N_002863"
     CDS             complement(1097..1363)
                     /locus_tag="ACG85N_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0225557.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1353..1709)
                     /locus_tag="ACG85N_002864"
     CDS             complement(1353..1709)
                     /locus_tag="ACG85N_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0225558.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          512 a          336 c          208 g          711 t
ORIGIN      
        1 ttctccataa taacagatat ctttttttca ttttttaaaa aatacctttt aaatttctta
       61 tagtcctctg aaatcaaaat atagcgtgat ggatactcat caaaagaaga atcgtattct
      121 tcctttaaaa ttcccgattt acttaatttt tctagtacat caaatactct atcactcaag
      181 taactcctta aattgttatc actttcccat tgaactactt ttctataagt tgaattatca
      241 gttgaaaaga tagaattttc atccataaac gcatatatta gcagcaatct ttcatcattg
      301 tcaaattcta ttggatcaaa aatatctaag ttgtcatcaa aatctttatc agttccggta
      361 acttttttgt taattactga aggtgtcttg taagaaatag aatctaaatc aaagcgtttt
      421 gattctaaat tattttgtgc tttctcaatt aaataattca ctttttttgt gagaaacctt
      481 ctgatattgt ctttgctata tgttccataa aaacccttat cttctaaaga gtccctgtac
      541 ttcttaactt ctatatactc ttcagccaaa acttgtggaa ctggttcatc taagaaaaaa
      601 acccctacct cgcctttgct atttttataa aagtaatcaa tttctgctat agttcccgat
      661 ctataattat caactggaga acccaactta tggccaaaaa tcgctaataa tatatcacta
      721 ttttctacaa tttgtttatt aataacactt tgcccagagc tattatttat ataagtagga
      781 atcgcattcg tttcccatcg aattggcatc aaaattactt ttgtattatc tgtattttct
      841 atattccatc tatatatggt ttcttctact tcattcctct ttgctgctat atcacctggt
      901 gatgctataa atacttttat tagatttatg gttttcattt attgtacctt tctctttatg
      961 tctctttaaa gtaatttttg tataaaattt attgactttt acactatagg acaactttta
     1021 tctatttagt acaaaaaggc ccactaaact aattgattag ttaggagcct attacaggta
     1081 tattttcaac tcgcttttaa gcaaattcta ctaccagctt tttgcctaat gccctagcga
     1141 tatcatcaag tttatcgaaa gatacgttat cccctctctc aattcgagca atagtagatt
     1201 gagttgtatg tgctttttgt gcaagctccg cttgagttag gcctgcttcc tcccgtgcat
     1261 gataaagagc aaccgcagtt tcgagttttc ttccttcacg gtcatatgct tctgcaaaat
     1321 ctttgtcttt ttgcttttct tcaatcaatt ttttaatcat catttgtgaa ccactcctct
     1381 ctaagttctt ttgcatgttt aagttcactt attggtgttt tctccgtttt tttagtaaat
     1441 ccatgggtaa tgacatacct tccattgacc acatggaaat agatagctct ttggatgttt
     1501 gatcctacct tacttcttaa ttcgtacaaa ttagtgtcta attttttcac ccatttcaac
     1561 cgttgagcta ctagcatccc attcttttct gtttcttcta ttgtacgtaa aagcttcgca
     1621 ctatcttttt ttggtaatgt ttccaaccat tctaaaaatt catcatgacc attaggtctt
     1681 ttaaatgttt caaaagatat tttttccata cccaattata tcatatattg attttatatt
     1741 atagtatata acataaaaat tcgaatt
//
LOCUS       DAZKLA010000225         1743 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000225 DAZKLA010000000
VERSION     DAZKLA010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.234"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            211..567
                     /locus_tag="ACG85N_002865"
     CDS             211..567
                     /locus_tag="ACG85N_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0225559.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            577..1476
                     /locus_tag="ACG85N_002866"
     CDS             577..1476
                     /locus_tag="ACG85N_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0225560.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          589 a          297 c          356 g          501 t
ORIGIN      
        1 gaataaggtt aaacaaataa caggttgctt tttgggtctt tttccgttca agaaactgaa
       61 cgaagaaaag ggaaagttgt ctcttttgtg ataagagaaa tcataataaa cgaatgactt
      121 actttccaat aaattgagaa cagtcagaac cagacaaatt agttgcatct gatgaataaa
      181 gtgattattc tatgacatga ggtgatacag atgaaattag aagtgacaaa agaagcacaa
      241 gaagtattga agaataaatt agaagcagac gatcaacttt tattagatat cgaaaacggt
      301 gacggtccat ttgcagaaag ccaagtcagt tgccaattgg atacggcatt tcgattgatc
      361 attgtcaaaa aagatacaca aacagatcta agtctttatt ctgtagtggc tgatacacca
      421 gttggaccaa tcaaaatcaa agacagtgca atactttaca tggatgatcc atccacactt
      481 gctttagaac caacttataa tagtttacaa ttaaaaggac caagcggctt gagaaaaggc
      541 aacttgcaag ttgttcgaaa agactagagg gataaaatgg gaaaattggc gatcatcagc
      601 gacctacatg tggacatcaa taaattcggc gaatcagagc tgatgcagct ttggcaagtt
      661 ttgcaaaagc acaagatcac tcggcttcat ctagccggag atacggctaa taaagtagac
      721 cgttgcctag ctgttgtcga atttttcgtc aataaaggat tatctacgac atttcatttc
      781 ggcaatcacg agttggcaga tgtgcccaat gaacagatga tggaccattt tccagaccca
      841 cattttttaa atgaacggta tattgcttta aatgagcaga ccgttctttt agggatgaat
      901 ggctggtacg actatcagtt ttccgaattg aaagattacg aggcaatcgt acgaatgaag
      961 cgtttgtatt ggtatgatcg gttcatccag cgtgggaaaa cagatctcga ggtcaatgca
     1021 gaaatgcttg cagctgcaaa aaatttgcta gaccagctgg aagcaaaaaa acttgaggtg
     1081 attttatcca ctcatttcgt tcctaaacgg gcatttatca tgtatcaatc tgctccttat
     1141 gagcgttgga acaaattaaa tgcattttta ggatcagaat cattcggaga attacttgac
     1201 cgttattcaa atatcaaaca agtagttttt ggccatacgc atcgtcgctt tgaagataaa
     1261 tccatcggtc acacgattta cagctgcaga ccttttggtt attattatga atggcagctg
     1321 acccgagatt ttgtcttgaa agaacagttg ctagacagct atgatcctat gaaattacgt
     1381 gtcctcttgc gtgcccatca cccagctttt caggcctatc aaacacaaca tttgcaagag
     1441 gaatttgaac aagcgatgac aatcgttccg tattgagaca gcattagtaa tgtgtaaaag
     1501 aaaatacctt gtaaaacata aggataagta agagcagtag atccatttat cgtggatatg
     1561 ctgctttttt tgcggaaaat aatcagcgaa tgtttatttg atcggatgat ttgtaactcg
     1621 cagagtacta gatgcaagcg tgtattcttt ttttgaacga taatcacaaa aaaaaatata
     1681 atatacattt tttatttaat tataattaat attgattgaa gtctaggcag tcaagtcgag
     1741 agt
//
LOCUS       DAZKLA010000226         1728 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000226 DAZKLA010000000
VERSION     DAZKLA010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.235"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1508)
                     /locus_tag="ACG85N_002867"
     CDS             complement(27..1508)
                     /locus_tag="ACG85N_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0225561.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          536 a          232 c          300 g          660 t
ORIGIN      
        1 aataagagga tgtgacaaga attttgtcac atcctctttg ttttgttctc ataacctttt
       61 ttcatataca gttcatgaac cgcttttatt aacttgccaa ataacactgt attgtgcgtt
      121 gtatccagat agaaagtctc cgtttcatct gaaattttct catgctgatc tgtaatgatc
      181 aaatcagcat gttctgaata tgcggtaatt ttaacagcat ctttgcgaaa taactgctgc
      241 aaatggcttt gaagatgata atttcctgta aactgtttgg ttatctgcaa atggatgagt
      301 aactgctctt tcgtttctgt ttgtagtaag tgttggatca attgactaag gtattgactg
      361 atttggtttc tttccccatt ttttgaaaaa attgtatcga ttttttctct taatttcttt
      421 tcctgtataa aagaactaga atgattggac gtggcttctg gaaagcaaag tgtagaaaaa
      481 ctactgctgg attcacccaa cagttcgaaa aaaacaactg ccaacacaag aaaataaaaa
      541 taaattggta ttttattttc taaatggttg agaggaaact gttgagttaa gtaagtcatc
      601 aattgaccga cttgtcgaca aataaggttt ttgcttgtgg aaaataaaaa accaatctcc
      661 tgtttttgtc gaaaagagat ttgatttgga aaaaaacaat agaaaaaata attaaagtag
      721 gctctctctg tttctgtctc ttcacaaggg cgactcactg aaaaagaagt aaagtattct
      781 gaataatcat gtgtatgctg caataagtcc atcaattcag tgatttcaga agaagttttt
      841 ggaaaatatg cctgttgttt gtttcgtaat atgaataaag ttaacagcaa gtagatattt
      901 ttttcttgtt ttgaaatttt ttctatttgc tcatctgtac tgttcatttt tagtgcattt
      961 aattcaagtg aggaaaatgg ccaatcgtta cctccaaatg tatctgaaag aaaaaagtaa
     1021 agatagagac ggatagataa ctcatttcct tcaagagtac agcaattttt tacagatagg
     1081 atccgtaaat ggaatttttt taaggaatga ttgagctgtt ttgtcagttt ttcgagatag
     1141 gagggcgata tataaagttg ttcaagtaaa tatttttttg gtacagcagt ttgtggataa
     1201 ttgattagta acatcaaata gttaaaacga ttggattctt ttaggtagca taattttaac
     1261 ttagaaaaaa cagctaactt tttagtttga tcggtttctt tggtttggat ctctccttta
     1321 tgattagaat aaataaattc atcaatcaat aaaaattgct tgagtgatac atttattttt
     1381 cggatcagtt ctttgattgt ccgttttgga atatgtaagt cattggataa ttcttgtaaa
     1441 gaacaattat ttttttgaat gaccatttgt agaacaagta actctttttt tcttgcttca
     1501 attagcataa atacctcctt tttattctgt ttttgtatat agttagacaa aaactgtgag
     1561 tggaaagtca tttcagtctg tgttattctc acagagtgtc gccttctttt ttatataacg
     1621 ttatttaatt tttttgttac cttgtttatt ctattgttta tttcctaggg tattttgtca
     1681 agatattctt gtttttaagg cggaaaaatt gatttttaat ggatataa
//
LOCUS       DAZKLA010000227         1726 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000227 DAZKLA010000000
VERSION     DAZKLA010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.236"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(100..1119)
                     /locus_tag="ACG85N_002868"
     CDS             complement(100..1119)
                     /locus_tag="ACG85N_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0225562.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1116..1340)
                     /locus_tag="ACG85N_002869"
     CDS             complement(1116..1340)
                     /locus_tag="ACG85N_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0225563.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1337..1630)
                     /locus_tag="ACG85N_002870"
     CDS             complement(1337..1630)
                     /locus_tag="ACG85N_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225564.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
BASE COUNT          484 a          354 c          291 g          597 t
ORIGIN      
        1 tgggtgatag tgaagatatg cgataacatc actattttat cgaatgattt ttatagttgt
       61 caatatagtt atgtaatgct cctcaacgag gagctatttt tatcgtttag gaatatttaa
      121 ataccaacgt ttgtcatgga aatcttgcgc accgccttta gtgtttccct ctggatcatt
      181 cgttgcccgc atcatgacat agactttctt attagggaag ttacgcatgt taaaagatac
      241 atgatagcca acatttccag aagtattata agcttgattt acatctgatc tataaattcc
      301 atcagctctt actcgagcta attctttccc agtattgtaa tccataatga agatatactc
      361 gtatttatag ttagcaatgt gccatccagc tacatgcaaa tttgcatttt cgatttctcc
      421 aaactgatca atgtgggcgt aattcgttcc atctgtcagt gtaggattag ctgcaccagc
      481 tcgtgttgga tcaatgacag gcttgttttc agaagttgtt ggattttcat cggtaaatcc
      541 atgagctaaa tcatatgcta atttttcttt acttacgccc atttcagaaa gataaccgta
      601 aggatctgta tgatcgcccc agatgttttg tgttacccat aaatgcgatt tgattcccgg
      661 ttggttataa ggagtgtcca acgttaatgg aataccatat ttcattgctg aatctctagc
      721 caattcaacg tatgccttgt agtttttctc aaacgttgct ttatcatgtg tgtgttgtaa
      781 ctcaatctgc acaggactgt tggcattagc atacgaacca gcaccgtact gtacataacc
      841 aggttgaccg acttgataaa caattccgcc gtctcccaca atataagcag tataagcgct
      901 agtccatgaa cgttgcatat actgcgcttc attgcgtcct gttgctgttt cattagccgt
      961 ttcatgcagt aaaatatact gattatttgc tacttgtgag ctaccttcat ttgcgcccaa
     1021 attaaattca ttgttgatag tataggcaaa tccgttaatt ggcaataaaa aaagagccgt
     1081 taacaggctc atcgcagtaa tagtaatttt ctttttcatt tgtttcctcc ttcttcgctt
     1141 tcagccgaga acattttgta ggttcgattt gatacaccca acacactccc taaaaacgcg
     1201 ccaaaaccag taatgatgac aacacagata tctgtgtact gccaattgag cgctttacca
     1261 actaacccca cgaaagtagc tagtgcggga ataattacca gtgcgaacca ttttagtact
     1321 tcgaacgttt tattattcat tttcttctct ccctaaacaa agttttgatt tgttgcgtgt
     1381 gttccaccaa tttttctgta tgtgtatcta atctttcatc gtgtttcttt agttcttcat
     1441 gaatcatcaa tcgatctgat ttgctcgatt ctaaatcttt agtcagcaaa tctaaattgt
     1501 ggcttacttt tgaaagagtc tcagtaattt tcgagaaaga tgcagcaatc ggtctaatta
     1561 ctaataaaat caaagaaacg atagcggtta ttgatcctgc tatcattccc cattccccta
     1621 aattaatcat gtgacaactc ctttacctta aataaaaacg catcaattaa gatgcgctct
     1681 tatctttatt aatgatttta tctgcttctt cgtctgtaat gcataa
//
LOCUS       DAZKLA010000228         1664 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000228 DAZKLA010000000
VERSION     DAZKLA010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.237"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG85N_002871"
     CDS             131..505
                     /locus_tag="ACG85N_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0225565.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG85N_002872"
     CDS             complement(619..1248)
                     /locus_tag="ACG85N_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0225566.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG85N_002873"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG85N_002873"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAZKLA010000229         1662 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000229 DAZKLA010000000
VERSION     DAZKLA010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.238"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            180..812
                     /locus_tag="ACG85N_002874"
     CDS             180..812
                     /locus_tag="ACG85N_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0225567.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            935..1573
                     /locus_tag="ACG85N_002875"
     CDS             935..1573
                     /locus_tag="ACG85N_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFQ0225568.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          559 a          252 c          262 g          589 t
ORIGIN      
        1 ttctaagatt tttattagaa ttacgcctaa tttgtgtctc tcagggcaat tctaagaacg
       61 tttcaagaga tagcgacact atcgttatcg aaaaaggcga aaacctatct cattttctgc
      121 taatgtaagt tggtttagct tttgttaata tgactttatg aaaatggagg taattaaata
      181 tgatttttcc taaagttaat gaagcaagct tgaataaagg atcctttttt catatctatg
      241 aaaacgaaga ttttgaactg tataatatcc tttcacaaaa agaggtcatc gaaaaccaaa
      301 taatctgcca tccaattatt tacaaatcta ttttcaagta tgcatttttt gcttccaatg
      361 attttcaaag aatggagatt accgactgta tattcgaaaa ctgtgatttt tcaggctgta
      421 tattgacaaa aagcatgttg catcgcgtga aatttgtcaa ttgtaaatta acaggtacta
      481 aattgatgga aagttatatt gggaacacgt tattcgagaa ttgtaaaatg gactatgtaa
      541 atttatctgg ttcaaacata aaagaaagta cctttgagca ctctgtctta tcaagcgcag
      601 attttgtaga ctgttccctc actaaaataa tgttttctac taatgattta gaaaatgcta
      661 gcttttttga tactgattta aaaaatatcg atttaagtag caactccttt gaaatgattg
      721 aagtacgctc ggaacatcta aaaaattgta cgcttaatca gttgcaagca cttggttttg
      781 ctagaaaatt tttacaaata aaagtagtat aatcaaaatg aatatataag ataaattttt
      841 agcaatacaa atatccataa aaaaacattg cctattttaa ctcttcatgt atactgactc
      901 tatcttcaaa aactgaatag taggagttat tatcatgaat aacattttag gaaaaaaaat
      961 agaacttaaa aaagggatat tgggcactat tgtcgttgta ctcggtttgt tagggttgct
     1021 atttgcgcca tccattatta gcattttttc tttaactatc actggattta tagcatttac
     1081 acttgttttt ggtaaaaaag atacgaaaaa aatgttctcg aaacctgtcg ctcctgtcaa
     1141 aaatatagcc aaatattttt ttatcaatgt agtaatcagt gccgcagtct cggtattttt
     1201 gcaacaaatt ttgaaatggg gtttaacagg caatccaata aatgaggctt tcagcccgtt
     1261 attatttatt attttgccta tcatgatact tggagaagaa ttgttctcag tgtattttct
     1321 cgctattttt tctagtaagt tcagcattcc tgtcgctagt ttcttgtcag caatcatctt
     1381 tggatttatc cattacagta cttatgataa tggaaatatt ttacacactg tagcacacat
     1441 ccttttgata caaggggtgg caagattgtt attcaaccaa gctgccatta aaagcaactc
     1501 gatcattaca agttgggcag ttcatgtgat atttgatttc acagctatat tgctagtcgt
     1561 tttattctcc taaattttta tagaaataat acatgctcta ttgctaagaa agcatacttt
     1621 atttccttta aacaaaaaga cagatgtatc aatggattaa aa
//
LOCUS       DAZKLA010000230         1653 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000230 DAZKLA010000000
VERSION     DAZKLA010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.239"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..1604
                     /locus_tag="ACG85N_002876"
     CDS             138..1604
                     /locus_tag="ACG85N_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFQ0225569.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          462 a          296 c          338 g          557 t
ORIGIN      
        1 tttttatgat attgccatta aaaattactt gcttttatag aaaattagtt tatatttctt
       61 aaataagttt tttagttaaa tttttcggta aagatgtata atattttctg aaaatttaaa
      121 gaattggggt atagattatg gaaaaacagc aagacaaggg cttcttcggg cagccgaagg
      181 gattgtcaac tttgttcttc actgagatgt gggaacgatt tagttattac ggtatgcgtg
      241 cattacttat ctattatatg tacgatacag ttgccaacgg gggacttgga ttaccacgag
      301 caacagcatt agctatcatg tcgatctacg gatctttgat ctacatgtct agtatcgtcg
      361 gcggatgggt atctgaccga gtgttaggag caaaacgcac tgtattcttc ggtggagttt
      421 tcattatgct tggccacatc gtcttggctt taccatttgg tgtaagtaca ttgtttgttt
      481 cgatggcatt gattattatc ggaaccgcct ttttgaaacc aaatgtttct ggcatggtcg
      541 gacatcttta ttcaaagact gacttgcgaa gagatgcagg attttcgatt ttttacatgg
      601 gtgtcaactt aggggcatta ttagcaccac taatcgttgg tacaatcggt caagaatata
      661 actatcattt agggttctct ttagctgcta ttgggatgtt ctttggatta ttacaataca
      721 tcatccaagg acgcaagaca ttagacggag tgggtacaac accgccgaat cctttgacaa
      781 ctgaagaacg taaaggattc atccgtaact tgatcatcgc acttgttgtt atcgctgcag
      841 tattcggcgg ggcacaacta actggtcatc tgacaattga cttttttgtc aatacaatca
      901 gtgtattggg gattatctta ccgttgtatt actttatcaa aatgctgact tcaaaagatg
      961 taactgctga agaaaaacca agagtatggg cttatatccc attattcctt gcagcaatca
     1021 ttttctggtc attacaagaa caaggttcat ctatcttagc tttatttgct agtgaacgta
     1081 cacaatctac gctatttggt gtaacgatcc aagcaagctg gtatcaatca ttgaatccaa
     1141 ttttcatctt tattctgaca ccagtattcg taacgatgtg gacgaaactt ggtgaacgtc
     1201 aaccatctac agtagtgaaa ttctctcttg gacttttatt tgcaggatta tcgtacttgc
     1261 tattgatgtt gccgggtatg ctttatggta caagtggaaa agtaagtcca ttgtggttgt
     1321 tgggaagttt cttcatcgtg gttattgctg aattgtgtct ttcaccagtt ggtttgtctg
     1381 tgacaacaaa attagcacca cgagcatttg aatcacaaac aatcgctatt tggttcttag
     1441 ctgatgcttc ttcacaagca atcaatgcac aaattgcacg cttctacaca ccgggaacag
     1501 aatctgctta cttcggtatt atcggtgcag tagcaatcgt cggtggtatc ttgctattta
     1561 ctgtgaaaga accaatcaaa aaactaatgg gctctattca ttaatcaatg aacataaaaa
     1621 caggaattgc ttatttgcag cagttcctgt ttt
//
LOCUS       DAZKLA010000231         1605 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000231 DAZKLA010000000
VERSION     DAZKLA010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.240"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..1542)
                     /locus_tag="ACG85N_002877"
     CDS             complement(64..1542)
                     /locus_tag="ACG85N_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0225570.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          517 a          292 c          221 g          575 t
ORIGIN      
        1 tttctttgaa aaaaacataa atcagctaat aaatatgtga gacaaagctg ccatccataa
       61 atactagctg attttttcag ttaaatgtct atttttttct ctgatgatgg tatttccaaa
      121 atattcccag tcatctggag tcggtggatt tttccaaatt atctgttctt tattaaattt
      181 ttcaagtaaa caatcagata aataaatatt cgtacgagaa gtgattcgac tctcaatcac
      241 aatattgtaa tttttaaatc ctttcacttg agaaataatg taatcggtat aagcagatcc
      301 ttgcgaaaaa tcaatacaga catgaatcgg tgcttcaaaa tatgataccg gacaaatacc
      361 gaccaataaa aagagataat cataaaataa gcgaatcatg aaacgttcat cttttcccaa
      421 gaaatagcgt ttattctctt gtatgacctt ttggattgct gtactgaata ttggatacgt
      481 ctcgaagaaa aactgtagct gctttttaga cgtaaagcta gtcatctcaa agtcgaatat
      541 ccaatttttt cgattgacat gcatcatacc catacgaagt tcttttcgta tatatagtct
      601 ttcttcttca gagatttcaa tcgaaaatgg taattgatca atcaattcca ctgacatttt
      661 ttgactaatc atgtggatat catgaaaatg agcttccgca agtccaaatg cgccatctaa
      721 ttcaaattct tcttccgtga tcaaaaacaa catgatataa gaaatctcat caatgatctg
      781 ctctttaggg atatgaagaa aagaaatatg tgataaatcc ttgatgatct tcattccttc
      841 tgagctctta aagtattcat ctgaaaagaa acgttttccg attggttgat tcccataggc
      901 cctcaacaaa ctaatagtgg ttagcaaatt gaatttgatt cgttttgttg cagacagttt
      961 taaattgaac agatattcaa aatattgcac gatactcttt gcttcattca tgacgctttc
     1021 tctaaatggc atagaaaaac cattaaagac cccgaaatat accccaaata atacgttccg
     1081 tatgtttatt tcttccccaa ctaatatgtt tttctttaac tttatttgat gctgttcaag
     1141 aaaagagact aattctcttc tttttacata ggctcttgat atgcttaaaa aatgattctc
     1201 tgcaaatttt tcgatcgtta cttttgaatc cacgatctcc gttaacagtc gagcaatttt
     1261 tgatcgttga aagtagattc ttcttaaatt tttgatatcc gtcaaattca gacgttcgac
     1321 atggatctct gcttcttctg aaatttggat ctctggcttt gttttgaatt cagtaaattc
     1381 tttatttaat tctatgatat aatttcttgt tttaaattta gacaaaccta atgtttcata
     1441 taacttaggc agcactacat aattcttttt ctcaaattct aaatatctag ctagcagcag
     1501 tttttctcta tcttcctctt ctataaggta atccaacttc atatcctaca cctcaaaaaa
     1561 tgcctcatac caattacttg atatgcagat gaaagttcat ctttt
//
LOCUS       DAZKLA010000232         1583 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000232 DAZKLA010000000
VERSION     DAZKLA010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.241"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            125..1351
                     /locus_tag="ACG85N_002878"
     CDS             125..1351
                     /locus_tag="ACG85N_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0225571.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(1459..>1583)
                     /locus_tag="ACG85N_002879"
     CDS             complement(1459..>1583)
                     /locus_tag="ACG85N_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HFQ0225572.1"
                     /translation="LSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT          582 a          241 c          328 g          432 t
ORIGIN      
        1 tttaaaaaat aataatcgtt acacaaaaat gttttttaaa gttttcttat gattaaaaag
       61 tgaatttgct gattgaacag ttatatgaga caatatttta gaaaatgtga aacagaggtg
      121 tattatgaag cgatggcaaa aagttctatt aaccttgtta ggggtattcg ttttgctgat
      181 tgccggatta tcagcatacg gaatcagatt tatgggagaa gcgagccaaa cagtaaacaa
      241 aatttctaaa aactctaatc gaatatcctc caaacgttct gagagagtaa gcattgatga
      301 caaagaacct ttttcggttt tgctattagg tttagatacc ggtggattag gaagaacgga
      361 acaaggacgt tctgatacga tgatggtagt aacagttaat ccgaaacaaa aaaaatcaac
      421 gatcatcagt ttggaccgag atatttatac gaatatcgta ggttatggaa cggtagataa
      481 attaaatcat gcctacgcat ttggtggtgt cgaaatggcc atggattcaa ttgagcaact
      541 gctcgatatt cctattgatc attatgtaac gatcaatttg gatggcatgg aagatttgat
      601 caatgcggtc ggtggcatca aagtgaataa taaaattgac tttactttgg atggtgtcca
      661 tgtaccagta ggtgaacaaa cattgaatgg agaaaaaggt ttagcctatt ctcgaatgcg
      721 acacgaagat ccagaaggcg atatcggtcg acaacgtcgt cagcgggaag tagtgacgaa
      781 aatcgtcaat aaagttttaa gtttagatgg cgtaagcaat tatagaaaaa ttttaaaagc
      841 agttgagaac aatgtgacga cggacttaga ttgggatgac atgcttgatg tagcgacgaa
      901 ctacaccccg gcgtttgaaa caatcaaaca agaccagttg caaggcgaag gacaaatgat
      961 caatgatatc tactatcaaa ttttaggaaa aaatgatctt ctttcgatcc aaaatgaact
     1021 gaagaaacaa ttaaatataa aaacaagcga tacattacct aatttgaaaa atgagaatgc
     1081 ttcaatgatg ttctttgatg attcggaaga tggcgattcg acaaacggtg atggatcgaa
     1141 tagtgaaggg tacaccgatt attcttccta ttcagaaaac acatatgatc agtaccaaga
     1201 tccgaatacc tattatcagc aaacagaaga tacaagtcag tactatacac caagtcaaga
     1261 tactcaaagt tatgatcctt acgctggaca ggaacaaaat ggttatggtt atgaccagaa
     1321 taatgagtat ggaacaacaa gtgggtacta gaaaaaaata gtttggttaa taaagagtgc
     1381 cacctaaatt agacaaaaaa tctaacttag gtggcacttt ttctatgtgt taaattacag
     1441 gacgtctctt tttagcgctt agtttaaaaa ataaattgaa taattcaata gagttgcgaa
     1501 tagtacccat aaataataag gaatcatgag ccacattgcc aggcgatgat gcagccacaa
     1561 ctgatacata agcactgaca aga
//
LOCUS       DAZKLA010000233         1580 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000233 DAZKLA010000000
VERSION     DAZKLA010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.242"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1580)
                     /locus_tag="ACG85N_002880"
     CDS             complement(<1..>1580)
                     /locus_tag="ACG85N_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225573.1"
                     /translation="KNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIG
                     LDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLT
                     MVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCY
                     EIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLK
                     ATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLF
                     LFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNG
                     FSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAP
                     EDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSN
                     NKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIA
                     SRYLSIVTLILFLLYRFT"
BASE COUNT          619 a          220 c          254 g          487 t
ORIGIN      
        1 aaagtaaatc gataaagtaa aaataaaatc aaagtaacga tagataaata tcttgaagca
       61 attgctagtt ttgtgtactg ataagtaata acgactttat ttatacctgg aggaatagtt
      121 aattcagttg tgccaaactt agataatttt gtttgtacta gtttgccatt aatagaagcc
      181 acttgccctt tataacgata aataggtgtc gtcaagacag tatttttatt attcgaatta
      241 tctaattcaa ttgtaagttt gttactcata aaatgagttg ttggatttat ttcctggtta
      301 cctagtaaat attgatcatt attaatcata tcaggatgat agattgactc tttgtttgca
      361 taatcagtat gattgtaatt ttcagctata gcaacagcgt cttcaggtgc tataagtgtt
      421 aatgaatctt tgttagcatg gatagaattg cttactcctg agagatgcaa tattagtgca
      481 agaaacgtaa agaccattat tttccatgaa ttatttttag tagaaaaaat ataatagatc
      541 actatactca tcgtataggc tataaataaa gaagaaaaac catttaaccg ccagacaaat
      601 tgtagactct taactggtgt atggttaaaa agttgccaag gaaataaatt ggttgaacaa
      661 aaaagaacaa atacaccaag aaagaaaata aataaatcat cagcagttaa tttttttata
      721 aaaataaaag caccaatcaa tccgagaaat aagaataatc caatagtata gttgttatag
      781 ttattattta aaatatgtgt cagcatatca ctaggagcca taccacttaa ttcttttccc
      841 agtgggactt tcaattcttg tgctctagat tgttgaataa aaggaattaa gaatcccgca
      901 cttaaaaaaa aggtgaggac ggtagctttt aaaagactta agagtctagc gattttttgg
      961 tcccaaaaat aaaaagaaag aaacaacgtt cctccaataa atacagaaac catagcgaca
     1021 gaaagtaaat gagtatacac tactagcgtc atgccaatag acaaccagta ccatttttgg
     1081 taatctctaa tataaatttc gtaacacccc attagtatca aaggcaaaaa tgttaaagcg
     1141 acagcttctc ccaatgaagc acgatggaat atgtcaatgg cacgataggc ggaaaatgta
     1201 taaataatcg aaaataacag cgaaatataa cgattgtttt ttatttgttt catagaaaaa
     1261 taagaaacga ccattgttaa aaaagtgata aaaaaatagt agatattata acttaaaaca
     1321 agatttccca tcattttata aaataaaaaa gctggataaa gagtaagcca tggataaaaa
     1381 atattcatca ttgttccatg atgatcgaag ttattgaagt ttactggaga actccataca
     1441 ttatctagtc ctatgattcg agataaatga aagtaagtat cctgattgac actatgatag
     1501 attcccttag acatgaatag agggatgacg taagcaaaac ttagaaataa cataaaaatg
     1561 atatctatta tttggttttt
//
LOCUS       DAZKLA010000234         1574 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000234 DAZKLA010000000
VERSION     DAZKLA010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.243"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..1255
                     /locus_tag="ACG85N_002881"
     CDS             86..1255
                     /locus_tag="ACG85N_002881"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0225574.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          570 a          276 c          250 g          478 t
ORIGIN      
        1 taagattaaa ataaatgtta tcaagataca agctatttta gctttattct ttctgtaaaa
       61 aagaaaggag actaaataaa ctattatgaa aaaaggaaaa catgctaaaa aaagaaaata
      121 ttcattttta atgatcgttg tcgtgttgtt tagccttagt gcgttctcta caggaactat
      181 tcttggatgg caattttaca ataatgaaca ctctcagata gttgcccatg ctcaatcagc
      241 tcaaaaaaag ttgatactga ctaccaatga acaacagctc gttgaaaaaa tcgatcaact
      301 attaagaaaa aatgattata ttggctcaat ttacgtaaag aaaaataatc gcatcatcat
      361 ggaaaaaggc tatggctatg cgaataaact cacgcaacaa ccaaatgatc cttctcttta
      421 ttatcagatt ggttcggttc aaaaagcatt gactactctt ttgattttaa aacaagtcga
      481 gcgtgggcac ctttctttag atactaagct ggctcaattc tatccacaaa ttgctggcag
      541 ccagcaaatt actatccgtg acttgctgta tatgcgctca ggcttgcacc gaactgcttc
      601 tccaaccata ccgatgactg atgaagaagt cgttcaattt gcgattcagc acttaaaatt
      661 tacagattat cagacctctc attatgattc attgaacttt accatattga ctggaatttt
      721 gatcaaatta acaaatcaac cgtatgaaaa attattgacc gatgatatca tcgaaccttt
      781 aggattacag catacaaaat tttatgacca agtcaaaaat tcatccaatc atgcactatc
      841 ctatcaaatg tctaaagagg atgattactt taaaccactg acagaatctg aaacagagat
      901 ccataacgaa ttagggactg gtaatatcag tatgtccgtc tatgatttaa atacgctgtt
      961 tacacaagtt ttatctggaa aactagtttc caaagactta ctcttctcat tatggcgtga
     1021 aaatgcgaat ggacatccat atagtggtgg ggtatatagc ggagataatt atatcctgtc
     1081 gcaaggaaat atcgactctt ttgatacatc tgctatattt agcaaagatt cacaggatgc
     1141 tatcatcatg gaaagcaata taaaagctga caaaactatc aaactgccta ctgctgatct
     1201 aagaaaccaa atctatgacc tgatggaagg aactactaag ttggcaaata gctgatatga
     1261 tgaaaagata gcaaatacta cttctttgaa tagcatttgc ttaaaaaaag tactcgacta
     1321 aacgtaggaa agagtgtggg acaaatctaa aaatcaattt ttgctacaga ctttaattcc
     1381 aaataaacag aacagaaaga ggtgttcttc tttctgttct gtccaaatta ttctaagatt
     1441 tctctttacc aacattattt gacaaagggc ctattaaatc taaaatagta tctttagatt
     1501 gctaaaatat gtgctagcaa atcattcatg gcttgttctt atttttaatt ttatgcaata
     1561 aaaaagaaga acaa
//
LOCUS       DAZKLA010000235         1565 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000235 DAZKLA010000000
VERSION     DAZKLA010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.244"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..911
                     /locus_tag="ACG85N_002882"
     CDS             3..911
                     /locus_tag="ACG85N_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFQ0225575.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            911..1531
                     /locus_tag="ACG85N_002883"
     CDS             911..1531
                     /locus_tag="ACG85N_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225576.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          561 a          224 c          356 g          424 t
ORIGIN      
        1 taatggcaaa gtggaatgtc atattaagta caacagaacc ctataattac gtggggatga
       61 ttcaagttcg acaaggcaat aagaatacag aggttatgga agcgactata gttgaaaatg
      121 gtcttcccta cgatttatca gaatgtaagg tatattttga atcagttgta ggtgggaaat
      181 atccagtcca attagaaaca aaaattgtgg atgctaaaaa agggaaaatt aactatattt
      241 ttgataaata ttccatgcag tgtttacatc gacaaacagc caatttcatt atatttaaag
      301 gagaagactt gattggaaca actcaagact tctcttattt tgtcattaat gctgtttcaa
      361 aaacagaagg agaaatgggt tcttattggc aatcaatcga agatttaatt gcggatatga
      421 cggactttat taatgaaaat aaaggcgatt ttacggactg gatgaatgaa aggaaagaag
      481 aatttgaacg ttggagacaa gaacaggaac acagtttcca agattggaga gaaggacaag
      541 aatcagacta tttggcatgg tttgaatcaa tcaaggatat tttaaagacg attgatccag
      601 gtggagtcat gttagccgaa ctaacggatg cgcgagtaga tattcaagga gttcgtcatg
      661 cgtctatttc agagcgtttg ctggcagata tggactattt atatcagaaa ttgcgagcaa
      721 cgcttttcac gattgaatac ggggagattg aagtgactga tattttgcag gatgatctct
      781 tttcagataa tcatgaagtc gaaaaagttg gaactgtagc attcccgatt gaagaaggag
      841 ccttgatcat cgcaaccgtt gatgatccaa agcaaaatgt gttcactctt gagaaagttg
      901 gggtgatcta atggctaaaa ctaaacgaat gatggaaacg gatgaaaaaa cgggtgtaca
      961 acgccaattc tttccaatca cacatgcttc cgcagttctt gggttagaag aaataatagc
     1021 aggagaagca acagttttat ctgttaatgg aaaaattgga gccgtcgtca ttactaaaga
     1081 agatttggga ttagagaatg ttctcacaga attaccctat gcaagtgaag aagatgacgg
     1141 cattatcacg gcagaaatgt atcaaaaaat tttaaacagt ggagaaggtg actacgtgtt
     1201 gccagttgcc actatcgacc gtttgggtgg cataaaagtt ggtgaattat tgacgattga
     1261 tgagacaggg aaagtctctg cagtcagaca atctgatgtc aatttttcgc tggagttaaa
     1321 agaaaaactc gattcactga aaaattatac tgctggagaa aacatcacta ttgatgaaga
     1381 tggaacaatt agttctacag gtggtagtgg gactggtggt gtcaatcagt catatgtaga
     1441 tcaaaaattc caagaagctg taaatcaagc agaaaattac acaaacgaaa gaattccaaa
     1501 ttttactttt gaaaaaattg gggaggtata gagaatgaca gatatcgtaa aaataaaaca
     1561 aagca
//
LOCUS       DAZKLA010000236         1547 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000236 DAZKLA010000000
VERSION     DAZKLA010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.245"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..445
                     /locus_tag="ACG85N_002884"
     CDS             191..445
                     /locus_tag="ACG85N_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0225577.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="ACG85N_002885"
     CDS             446..763
                     /locus_tag="ACG85N_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0225578.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1322..>1547
                     /locus_tag="ACG85N_002886"
     CDS             1322..>1547
                     /locus_tag="ACG85N_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225579.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKK"
BASE COUNT          549 a          221 c          295 g          482 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acatccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac
     1381 tttcaaaaga ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata
     1441 caagtttaat tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc
     1501 tgagagtagg ctttttcatt ttagaataag ttctcatgta aagaaaa
//
LOCUS       DAZKLA010000237         1537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000237 DAZKLA010000000
VERSION     DAZKLA010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.246"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            230..1399
                     /locus_tag="ACG85N_002887"
     CDS             230..1399
                     /locus_tag="ACG85N_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0225580.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          565 a          219 c          250 g          503 t
ORIGIN      
        1 tatgtttggg ttttatagca aagaaagttt aataagagtt tggacctagc aaacaatatt
       61 aatgaggtca ttaaatctta ttatctttag tgatataacg aaatatctac acagacaaaa
      121 tgatagcgta aaatattttg tgtaaataga tatttagggt agaaaaaaag aaagtccatt
      181 ctgtaaaatt aaagttacca caaccgaaat tttataggag gactttctca tgcccaattt
      241 tactacagaa attatggaaa cactaatcaa taaaggtgat ttagatgaat tatttcgatg
      301 tcatttagaa ttagctgtta attcattatt gcaggctgaa ttaacagctt ttcttgatta
      361 tgaaaaatat gatcgtgctg ggttcaattc aggtaattcc cgtaatggga attattcacg
      421 ttcattcaga acagagtatg gagaattaaa tttggtaatt cctagagata gaaatggtaa
      481 gttttctcaa caaactttac ctgcttataa aagaaccaat gactctttag aaaccactat
      541 tattcagcta tttcaaaaag gaattactat gtctgaaatc tctgaattga ttgaaaaaat
      601 gtacgggcat cactatacgc cacaaaccat ttcaaatatg actaaaatag tttctgaaga
      661 tattattgcc tttaaagaaa ggtccttaga atccagatac tctgtcattt ttatggatgc
      721 tactcacatt cctttaaaaa gacaaactgt ttcaaaagaa gcagtttata ttgtaattgg
      781 cattcgatta gatggaacca aagaggttct tgggtttagt cttgctccaa ctgaatctgc
      841 ttatgtttgg aaagagattc ttcaagacct aaaagaccgt ggtttaaaag aggttttatt
      901 agtcgtaact gatggtttaa gtggcattaa cgatagtatt catagtgttt atccaaatgc
      961 tcaatttcaa caatgttgtg tccatatatc tagaaatatc gctcataagg ttcgtgttag
     1021 tgatcgacaa gaaatctgta gcgatttcaa attagtttat caagcaagtt caaaagaaga
     1081 agcaaataat caaataaggt ttatgataga taaatggaaa aagcagtatc cacgagtggt
     1141 taaattgctc atgaatcctg ctatattaac cttttataat ttccctccgt caatcagaag
     1201 aactatttat tcaactaatt tgattgaagg ttttaacaaa caattaaaga aatataccaa
     1261 aagaaaagag caatttccta atgaagaatc tctggagaga tttctagtat ctcaattcaa
     1321 caactataat caaaagtttc tatgccgagt tcataaaggt tttaaagaaa tacatgatac
     1381 attagaatca atgttttaac ttgatcaaca gaatgggctt ttccatttac acaaaattat
     1441 tgacgctccc gacaaaatta tgatcttgcg taaagttata aaatattaat aaaaatattt
     1501 tattaatgaa aagcttggta gcacggttat tcatatg
//
LOCUS       DAZKLA010000238         1536 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000238 DAZKLA010000000
VERSION     DAZKLA010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.247"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1536)
                     /locus_tag="ACG85N_002888"
     rRNA            complement(<1..>1536)
                     /locus_tag="ACG85N_002888"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          321 a          476 c          337 g          402 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccag
//
LOCUS       DAZKLA010000239         1493 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000239 DAZKLA010000000
VERSION     DAZKLA010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.248"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..143)
                     /locus_tag="ACG85N_002889"
     tRNA            complement(59..143)
                     /locus_tag="ACG85N_002889"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(107..109),aa:Leu,seq:caa)
     gene            complement(161..232)
                     /locus_tag="ACG85N_002890"
     tRNA            complement(161..232)
                     /locus_tag="ACG85N_002890"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(198..200),aa:Gln,seq:ttg)
     gene            complement(242..314)
                     /locus_tag="ACG85N_002891"
     tRNA            complement(242..314)
                     /locus_tag="ACG85N_002891"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(279..281),aa:His,seq:gtg)
     gene            complement(376..460)
                     /locus_tag="ACG85N_002892"
     tRNA            complement(376..460)
                     /locus_tag="ACG85N_002892"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Leu,seq:caa)
     gene            complement(481..551)
                     /locus_tag="ACG85N_002893"
     tRNA            complement(481..551)
                     /locus_tag="ACG85N_002893"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Cys,seq:gca)
     gene            complement(597..668)
                     /locus_tag="ACG85N_002894"
     tRNA            complement(597..668)
                     /locus_tag="ACG85N_002894"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(634..636),aa:Gln,seq:ttg)
     gene            complement(680..752)
                     /locus_tag="ACG85N_002895"
     tRNA            complement(680..752)
                     /locus_tag="ACG85N_002895"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(717..719),aa:His,seq:gtg)
     gene            complement(755..828)
                     /locus_tag="ACG85N_002896"
     tRNA            complement(755..828)
                     /locus_tag="ACG85N_002896"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(794..796),aa:Trp,seq:cca)
     gene            complement(835..915)
                     /locus_tag="ACG85N_002897"
     tRNA            complement(835..915)
                     /locus_tag="ACG85N_002897"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(879..881),aa:Tyr,seq:gta)
     gene            complement(917..992)
                     /locus_tag="ACG85N_002898"
     tRNA            complement(917..992)
                     /locus_tag="ACG85N_002898"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(957..959),aa:Phe,seq:gaa)
     gene            complement(1028..1100)
                     /locus_tag="ACG85N_002899"
     tRNA            complement(1028..1100)
                     /locus_tag="ACG85N_002899"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1065..1067),aa:Asp,seq:gtc)
     gene            complement(1110..1182)
                     /locus_tag="ACG85N_002900"
     tRNA            complement(1110..1182)
                     /locus_tag="ACG85N_002900"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1147..1149),aa:Val,seq:tac)
     gene            complement(1198..1269)
                     /locus_tag="ACG85N_002901"
     tRNA            complement(1198..1269)
                     /locus_tag="ACG85N_002901"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1234..1236),aa:Glu,seq:ttc)
     gene            complement(1284..1373)
                     /locus_tag="ACG85N_002902"
     tRNA            complement(1284..1373)
                     /locus_tag="ACG85N_002902"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1337..1339),aa:Ser,seq:gga)
     gene            complement(1413..1486)
                     /locus_tag="ACG85N_002903"
     tRNA            complement(1413..1486)
                     /locus_tag="ACG85N_002903"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1450..1452),aa:Asn,seq:gtt)
BASE COUNT          435 a          390 c          335 g          333 t
ORIGIN      
        1 cataaaaaca aaaaaaatat agcaactctc tataagaaag ttgctatatt gataattata
       61 ccggcggccg gggtcgaacc ggcacgtcct caacggacac tggattttga gtccagcgcg
      121 tctgccaatt ccgccacgcc ggcaaattta aaaataataa ctggggtagc tggattcgaa
      181 ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc caagaataaa
      241 aggcgaatga tgggaatcga acccacgaat gccggagcca caatccggtg cgttaaccac
      301 ttcgccacat tcgccatcat aaaagatgtt atttaatttc tcaataaaaa actaagatga
      361 agacatctta gtcaataccg gcggccgggg tcgaaccggc acgccctcaa cgggcactgg
      421 attttgagtc cagcgcgtct gccaattccg ccacgccggc aagttgattt attagtacta
      481 aggcggtaac cggatttgaa ccggtgatga aggttttgca gacctctgcc ttaccacttg
      541 gctataccgc cattatacta ttatcaatct ataaaataaa gaataaatta tagaaactgg
      601 ggtagctgga ttcgaaccaa cgcatgaggg agtcaaagtc ccttgcctta ccgcttggct
      661 ataccccaag aataaaaagg gcgaatgatg ggaatcgaac ccacgaatgc cggagccaca
      721 atccggtgcg ttaaccactt cgccacattc gccatggcag gggcagcagg aattgaaccc
      781 acactaacgg ttttggagac cgctgttcta cctttaaact atgcccctat aaaatggagg
      841 agagtggatt cgaaccactg aaccctaagg aacggattta cagtccgtcg cgtttagcca
      901 cttcgctact cctccatggt ggcgcgggac agaatcgaac tgccgacaca cggagcttca
      961 atccgttgct ctaccaactg agctaccgcg ccatatttta aaattattaa ttgaataaca
     1021 agaacgacgg ttccgacggg aatcgaaccc gcgatctcct gcgtgacagg caggcatgtt
     1081 aacccctaca ccacggaacc agttcattat ggaggttaac gggatcgaac cgctgaccct
     1141 ctgcttgtaa ggcagatgct ctcccagctg agctaaacct ccaacattca attaaaatga
     1201 cccgtacggg attcgaaccc gtgttaccgc cgtgaaaggg cggtgtctta accgcttgac
     1261 caacgggcca ggtaaatttg ctacggagag taagggattc gaacccttga gacagtttgg
     1321 caccatctac atgatttcca atcatgctcc ttcggcctct cggacaactc tccagagtga
     1381 aagaagctca atactctttc tcaaaatgag aactccggca gtaggactcg aacctacgac
     1441 atcatgatta acagtcatgc gctactacca actgagctat gccggaataa cat
//
LOCUS       DAZKLA010000240         1479 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000240 DAZKLA010000000
VERSION     DAZKLA010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.249"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..828
                     /locus_tag="ACG85N_002904"
     CDS             <1..828
                     /locus_tag="ACG85N_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFQ0225581.1"
                     /translation="NTEVMEATIVENGLPYDLSECKVYFESVVGGKYPVQLETRIVDA
                     KKGKIKYIFDKYSMQCLHRQTANFIIFKGEDLIGTTQDFSYFVINAVSKTEGEMGSYW
                     QSIEDLIADMTDFINENKGDFTDWMNERKEEFDRWREEQENSFQDWREGQESDYLSWF
                     ESIKDILKSIDPGGVMLAELMDARVDLQGVRHESISERFLSDLNYLYQKMKAALFTIE
                     YGEIEVTDILQDDLFSDNHEVEKIETVEFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            828..1445
                     /locus_tag="ACG85N_002905"
     CDS             828..1445
                     /locus_tag="ACG85N_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225582.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          544 a          192 c          311 g          432 t
ORIGIN      
        1 aacactgaag ttatggaagc tacaattgtc gaaaatggtc ttccatatga tttatcagaa
       61 tgtaaggtat attttgaatc agttgttggc ggaaaatatc cagtccaatt agaaacaaga
      121 attgtggatg ctaaaaaagg gaaaatcaaa tatatttttg ataaatattc catgcagtgt
      181 ttgcatcgac aaacagccaa tttcattata tttaaaggag aagacttgat tggaacaact
      241 caagacttct cctattttgt catcaatgct gtttcaaaaa cagaaggaga aatgggttct
      301 tattggcaat caattgaaga tttaattgct gatatgacag attttattaa tgaaaataaa
      361 ggcgatttta cggactggat gaatgaaagg aaagaagaat ttgatcgctg gagagaagaa
      421 caggagaata gtttccaaga ttggagagaa ggacaagaat cagattattt gtcatggttt
      481 gaatcaatca aggatatttt gaagtctatc gatccaggtg gagtaatgtt agccgaatta
      541 atggatgcac gtgtagatct tcaaggtgta cgccatgaat cgatttctga acgtttccta
      601 tccgatctaa attatttgta tcaaaaaatg aaagcagcac ttttcacgat tgaatacggt
      661 gaaattgaag tgactgatat tcttcaagac gatctctttt cagataatca cgaagtcgaa
      721 aaaattgaaa cagtagaatt tccaattgaa gaaggagctt tgattattgc aactgttgat
      781 gatccaaaac agaatgtttt cactcttgag aaagttgggg tgatttgatg gttaaagtaa
      841 agagaatgat ggagactgat gaatctggtg ttaaacgtca atttatgcca attacacatg
      901 tttctgcagt tttaggctta gaaaaaatga tatcaggaca atcaaaagtt ctttcggtta
      961 atggtaaata tggtgcagtc attttaacga aagctgattt aggattagag aatgcaatta
     1021 ctgaactacc ttatgcaagc gagacaagcg atggtattct tactgctgaa atgtttcaaa
     1081 agatcgtaaa tggagaaggt ggaacatata ttttgccgat tgctactcct gaacaattag
     1141 gaggtataaa agttggggaa ctcttagaag ttactgaaga aggtgtttta tctgcaacta
     1201 agcaaacaga cttcaatttc tctgaagaat taaagggaaa attagaatct ctaaaaatat
     1261 taaaagcagg tgcaaatata tcaattgcag aagatggaac aattagttct acaggtggta
     1321 gtgggactgg tggtgttaat cagtcatatg tagatcaaaa attccaagaa gctgtaaatc
     1381 aagcagaaaa ttacacaaac gaaagaattc caaattttac ttttgaaaaa atcggggagg
     1441 tatagaaaat gacagatatc gtaaaaataa aacaaagcg
//
LOCUS       DAZKLA010000241         1458 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000241 DAZKLA010000000
VERSION     DAZKLA010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.250"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..403
                     /locus_tag="ACG85N_002906"
     CDS             5..403
                     /locus_tag="ACG85N_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0225583.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="ACG85N_002907"
     CDS             387..1082
                     /locus_tag="ACG85N_002907"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFQ0225584.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          551 a          211 c          294 g          402 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctaactatta cttatttctc
     1201 atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt
     1261 tttgaataaa tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc
     1321 tctagtataa cagaataaga agaacaagtc cagaagggat atttttagtc ttttttaaaa
     1381 taacttttca gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa
     1441 aaaataggga agcatgaa
//
LOCUS       DAZKLA010000242         1421 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000242 DAZKLA010000000
VERSION     DAZKLA010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.251"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..1399
                     /locus_tag="ACG85N_002908"
     CDS             68..1399
                     /locus_tag="ACG85N_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0225585.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT          457 a          212 c          262 g          490 t
ORIGIN      
        1 ttgataagtg atgtattatt tgggtggaaa aagtaatcga ttacaataag cataaaggag
       61 aaaaaatatg ggcaaaagta gcaggaagga cattgtattt gaaaaatttg gtatagttgc
      121 gactaagttg gggaatcaga tccatttaag aactttgcga gatgcgtttg ctacatttat
      181 gccatttatg atgttagcag gatttgtgac acttattaat tcagtgatct tagaaccaac
      241 aggattcatg ggggaaattg tcgatactag tacattgacc acgttacaac aaattggtac
      301 ttcaattgcc aatggtacat taagcataac tacattatta gttgtagcag cagtatctta
      361 tcatatgtgt gcaagcagaa attatacaaa tcatattgca gcggtattag ttgctatttc
      421 gacatttgtt gtattaacac ctatgcagat gatgtttact cctgaaaatg cggataaagc
      481 tgttgaagtg acgggtgtga tacctatatc ctatacagga gcttcaggaa tgtttgtagg
      541 tatatttgtt ggattagcgg ctacagaatt atttattaaa ctttctaata ataagaaact
      601 acagattcag ctatccggaa atattcctcc cgctgtttta aagtctttta atgtattaat
      661 cccaatcatt attacgatta cctcgtttgc tttattgtca tttataatca ttcaattgtt
      721 taatatggat ataaataagc taattaccac gatagttaca gagccattaa gtaaaattac
      781 aactggttta ccaggatttt tgttaattac atctattgct aatctatttt ttggatttgg
      841 tatccatcaa gcagtaatat caggttcatt gttagatccg tttttgattc agaatatgca
      901 agaaaatatg gcagcttatg caaacaaaga gcaaattcca catattatca atatggcctt
      961 taaagatacc tttgcggtaa tgggtggatc gggaaatacg attgctttat tgattgcaat
     1021 atttatcttc agtcgtagaa aagattataa ggactttgct aaattatcgg taactcccgc
     1081 tatattcaat ataagcgaac ctattatttt tggattgcct attgtattta atatcagtct
     1141 gattattccc tttgtgcttg caccgatttt ttctttgaca gtagcatatt ttgcaactgc
     1201 tgtgggatta attaatcatg tagtagtgca aattccttgg actacgccac cagttatatc
     1261 gggattttta gcaacagccg gagactggcg agcagctgtt ttacaaatat taattatagc
     1321 cgcaagtgtg tttatatatt taccgttttt acgaattgac gaacacgtga caactaaatt
     1381 aagtgaaaat gaaacataat ttactatgag ataacaaaaa t
//
LOCUS       DAZKLA010000243         1417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000243 DAZKLA010000000
VERSION     DAZKLA010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.252"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG85N_002909"
     CDS             complement(150..1337)
                     /locus_tag="ACG85N_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFQ0225586.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAZKLA010000244         1362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000244 DAZKLA010000000
VERSION     DAZKLA010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.253"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1362
                     /locus_tag="ACG85N_002910"
     CDS             82..>1362
                     /locus_tag="ACG85N_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFQ0225587.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DAZKLA010000245         1352 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000245 DAZKLA010000000
VERSION     DAZKLA010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.254"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..587)
                     /locus_tag="ACG85N_002911"
     CDS             complement(45..587)
                     /locus_tag="ACG85N_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0225588.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(824..1222)
                     /locus_tag="ACG85N_002912"
     CDS             complement(824..1222)
                     /locus_tag="ACG85N_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0225589.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT          491 a          245 c          196 g          420 t
ORIGIN      
        1 ttattgttta atccaaaatc agtttactac cttagtacgt aaaatcaaac agctcgttta
       61 aacacttttc tagcctctaa agttttgata aatgaataaa caaccattat ttgaataggg
      121 atttggatca tattcctgat gaatctacct agaaaaatta ctttgaaatc tgcaccaaac
      181 aaaacagaca tcagccaaga attgataaaa aggctgactg ggatcataat caataattgg
      241 gcagcgatga cggaaggcag gtttatcttt ttatgatata aaaataagcc attaataaat
      301 ccaccaagga aagccgtgat aagaaaagca gcttgaaacg ttcctgaacc aaatatcgtg
      361 aaatttaaag tgttcgacaa catttcaagc aaaccagccc agatcggtcc atatagataa
      421 cccataagcg ccatacttaa aaatccgaat gtgatttgaa aataagcagg accaatcgct
      481 atcttgtata agatgacatc aagggctaat aaaagagcag aggtcgacaa caatcttgca
      541 ttcatttcgg gcaatctaaa aacgtctttt gcaatcaaag tgttcaaaat attctctcct
      601 ttgtcagtta ataataaaag gataccataa aatcaccaat gtgtcatgac acattggtga
      661 tttacataca ctggcatcta acaattaaaa aaagggtgag aagactgctt ttataaactg
      721 taaaaaagag attgtacgaa taaaaaaaat agaggcggag aatacttatc gtatcatccg
      781 cctttgtttt tttgttataa aaaacgtcag attagttatc tgcttacatc ttcatccata
      841 cgggttctaa tgtatttccg tctagatctt ggacttctaa tgaaaacatt tcttcttcag
      901 gcattcccat atctacttcg tggaaagtac caccattttc ttttgcaatt tgagcaaatt
      961 tttttacttc atccgcattt tcaacactaa acgaaatcaa agcaccactt gtttttttat
     1021 tatcagctat ttctttatct tttataaatt ttttataaaa atcatgagag agaagcatta
     1081 tccaaaaagt gtcatcccat accattgaac cagcttcttc agtagaaaat tcttcatttt
     1141 ttttaaaacc cagcttttca taaaaagcaa cggaacgatt gacatcagca acaggaaaat
     1201 taacaaaaac catactattc atttgtttcg tctcctttct gttttgttcg tctatcatct
     1261 cttatttagg aaacgctgtc aaaaatttag attcatatat agaaaccaaa ataaaaattt
     1321 gcatcatcaa aaaagtgaag atgtgataga at
//
LOCUS       DAZKLA010000246         1324 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000246 DAZKLA010000000
VERSION     DAZKLA010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.255"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..820
                     /locus_tag="ACG85N_002913"
     CDS             278..820
                     /locus_tag="ACG85N_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225590.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..>1324
                     /locus_tag="ACG85N_002914"
     CDS             976..>1324
                     /locus_tag="ACG85N_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFQ0225591.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          533 a          157 c          248 g          386 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccag
//
LOCUS       DAZKLA010000247         1300 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000247 DAZKLA010000000
VERSION     DAZKLA010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.256"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..638
                     /locus_tag="ACG85N_002915"
     CDS             <1..638
                     /locus_tag="ACG85N_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0225592.1"
                     /translation="EKNYIDFCDITTIIGWFLAISCACFITLFQPFMIIWVGSDNLMS
                     ISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNIILVNWFGLY
                     GVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVASYLSYFACS
                     YVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLVKR"
     gene            816..1037
                     /locus_tag="ACG85N_002916"
     CDS             816..1037
                     /locus_tag="ACG85N_002916"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0225593.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT          440 a          126 c          268 g          466 t
ORIGIN      
        1 aagaaaaaaa ttatatagat ttttgtgata ttacaacaat tataggttgg tttctggcaa
       61 ttagttgtgc atgctttatt acattatttc agccgttcat gattatctgg gtgggaagtg
      121 ataatcttat gagtatttca tttgttatat ttatgatagt tcattttttc ttatatgaaa
      181 ttgatcagtt aattgggtta tacaaagatg ctgctggaat atggtacgca gacaggttta
      241 gaccattaat tacagcaatg ataaacattg tattaaatat catcctcgtt aattggttcg
      301 gattatatgg agttttggcg gcaaccattg tttcagtatt gttggttgat ttaccctggt
      361 tattatataa tgttttccaa aatatattta aatttgaatc ggcaggtaaa tatgtttgga
      421 agctcataag tttgtgtgta atagcttttg ttgcttctta tttaagttat tttgcttgtt
      481 cgtatgttaa tatcggaaat ttcaaagtga taagtttagt gataaattta gttatatcgg
      541 ttgtcatatc gattttagtt attcttctgt ttaatatccg taataagaag tttaagtata
      601 ttttgaaagt tcttacgtcg gtattggtaa aaagatgaga agagacatca gaatgatata
      661 taaagtaact acaattttta ggctgccgaa taggcgaacg atgtatagtt tttgttccaa
      721 cgaagactct tgttgaaatt ggagatgata tgaaggagtt acaattttga ctcatgttta
      781 tgattgatcg gttttaaaag tagtatggag aagctttggg atcatcggag aggatcaaaa
      841 taggtaataa tgtttttata ggaatgaagt ctagaatact aaagggtgta catataggaa
      901 ataatgtagt tataggtgca aatagtttag taaacaagga cgtatcagac gattgtgttg
      961 ttgcaggaaa tctagcaaaa gtaattatgt ccctagatga atattataaa aagataaaca
     1021 atatgaagaa tccgtaaaac ttgtgaaact ataccgagaa taatatggga aaaatattga
     1081 tgaaaaagca ttatatgagt ttttagggct gtttacagat ggagatgaca atttgtcgaa
     1141 gagttgggag caatgcaaaa tttagtaggg gattataaac agactgacaa ggtatttaaa
     1201 gaacatgcac aagtatttca taatattgaa gaatttttga atactgtgag ataggagttg
     1261 ctacattgag aaaagtatgt atttttccaa tgtaaaagat
//
LOCUS       DAZKLA010000248         1294 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000248 DAZKLA010000000
VERSION     DAZKLA010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.257"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1244
                     /locus_tag="ACG85N_002917"
     CDS             <1..1244
                     /locus_tag="ACG85N_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFQ0225594.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAZKLA010000249         1289 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000249 DAZKLA010000000
VERSION     DAZKLA010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.258"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(255..869)
                     /locus_tag="ACG85N_002918"
     CDS             complement(255..869)
                     /locus_tag="ACG85N_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0225595.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          419 a          257 c          213 g          400 t
ORIGIN      
        1 ctttttccac tactttttat ttttctgaca ctacatattc ttataactca tttctcataa
       61 ctgtgaaaca acttataaaa ataacaaact ttaaacaaaa caagcttaaa atcaaagatg
      121 atccatctta aaataactaa aacaaaaaaa gtagtcgttg ttaatcctca ttaataatcg
      181 ttttcttttt ttcaaacgct ccatttttat gtggaacatt atttttttaa ggaactttgt
      241 caacatgtgg ataactattt tccacttgtg tcctggacta gctgttttgc tgtaaaatac
      301 aaaacttgct gcagcaaaaa gatcgttgca agagaggctg ttccgcagat cgctggatct
      361 agatttggca aagtcaagac tgcatcatgt aaattggcgt ggttgtccaa aaccaaatca
      421 gcaaattcaa ataatcgcaa accactgcta tgttgcgatt tgatacttcg tgacaaatca
      481 aatccagtaa caatgattaa tggatgacca ttttctttga tccactgagc tagttcgatg
      541 attgccgggt ttcttccttc attcgaaaga agaagaaaaa tgtcttctga tttgacagaa
      601 agctgatcaa taattgcctg tcctgcacct tctagcgttt catagacacc tccggctgga
      661 ttttcaatca atatacttgg tacaagatcc ggggtttgat cccaaaattc aaatgctaca
      721 ccacttagtg tgcggctagc atatatctgc aatctaccac cttgattaat ttgtttcgcg
      781 atccaagtgc cagcttgaag catcagcggc tcttctatcc ttaatttctc ttccaattgg
      841 tgataatagt caaaataaca taaactcatt tatctctccc gcctagctat acttgacatg
      901 aatagaaaca gctgcctaat ctaaggaagt acataaaaaa acagtagtta gacagtaaca
      961 catgtggtct ttgctgtttc aaacacaaaa aaggcattga acgaatacgc ccaatacctt
     1021 gacatagcaa ataaaacaac gtgcagacca tggaaaatat ctacataggg gtagaatttt
     1081 caccctagat taggtacaat aaattaggtc gatgcactgc attgactatg gcaaaatgat
     1141 cggtcagggt gtatgtgtgt gactggtcat tccctgattc ttagcgccaa ctaagggtca
     1201 gggagccttt ttttattcag ctgtttctat ctgaatttat tgtaagtaat ctcttatcca
     1261 agtgcaagca agaaaataca aaaaaacat
//
LOCUS       DAZKLA010000250         1278 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000250 DAZKLA010000000
VERSION     DAZKLA010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.259"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1042
                     /locus_tag="ACG85N_002919"
     CDS             <1..1042
                     /locus_tag="ACG85N_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0225596.1"
                     /translation="EEKIIQYDGLAKLKDAKVVSGEQRINREDLSDEFKNVVSLEATN
                     NTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAKQNNLKLGDEVDLELLDIEKSG
                     KIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDYSTSQEILNNSENNEIANKILM
                     YSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEESLESVSGIKYIIKVMTYSIMLG
                     GMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQFIFELIFISIPSIISSLFLGNV
                     LLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQSYLILISIIVLSVVFASSLML
                     IKKPKEILSKIS"
     gene            1052..>1278
                     /locus_tag="ACG85N_002920"
     CDS             1052..>1278
                     /locus_tag="ACG85N_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0225597.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYS"
BASE COUNT          529 a          115 c          215 g          419 t
ORIGIN      
        1 tgaagaaaaa ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag
       61 cggagaacaa agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact
      121 cgaagctaca aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa
      181 agaaggtaaa aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc
      241 taaacaaaac aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa
      301 aagtggaaaa ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca
      361 ggaaacatat acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc
      421 aacaagccaa gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat
      481 gtattctggt agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat
      541 tgatgagtca aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc
      601 agtgagtgga ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat
      661 ggttgttctt tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat
      721 attcttatct attggaacat ctaagataca aattataatg caatttatat tcgagttaat
      781 attcatatcg ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt
      841 aattgtagat ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa
      901 taatagtagt tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag
      961 tattattgtt ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga
     1021 aatcttatca aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc
     1081 atatagttat gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact
     1141 tgggaagttt tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt
     1201 acttgctgga cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca
     1261 aaataaagga tatagtaa
//
LOCUS       DAZKLA010000251         1275 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000251 DAZKLA010000000
VERSION     DAZKLA010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.260"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(489..863)
                     /locus_tag="ACG85N_002921"
     CDS             complement(489..863)
                     /locus_tag="ACG85N_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0225598.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(886..>1275)
                     /locus_tag="ACG85N_002922"
     CDS             complement(886..>1275)
                     /locus_tag="ACG85N_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HFQ0225599.1"
                     /translation="DYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLT
                     FGKTPNYPWPQMFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVG
                     TALSMTVTEGAIKVDKLDYSINYECGF"
BASE COUNT          430 a          236 c          164 g          445 t
ORIGIN      
        1 agaaaacctc cagacttctt gatagctcaa gtatatcttg aggtgcattt gttagtaagg
       61 tactgttcta tggggcgctt actctaggag tgagcatttt tcgaaaaata ctaaaaattt
      121 ttcaaaataa aaaaaggcat acagacaata aactgtctat acgcctcaca gactctattc
      181 tatacatcta attttggctg tcctgcaaaa aatatgtgtt agcctccatc gaaagatcaa
      241 actttggaaa acatcattat aactttgctt ggtaagagct ttgctaaaac atgatatgac
      301 ttgtatacaa aatgtggagt gtattccaat aaataggaag gattacaaaa tttgaaacca
      361 aataagtaaa aaatactgtc actttgaatt aatttgaaat agtaaattct cattaaacta
      421 ttgaaatgct gataccaaaa aatgaagtac ttttcgttca taccaactta gagcacttcg
      481 ttatctattt atttcttcat ttggcttatc tctataatct catgatttgg tccgtagaaa
      541 ttaaagtaac ggattccatt ttcccagaac ggtaattcca taatattttt atctaataat
      601 tcatatccta aatcttgaat tgaattgaat acctcatcta tgtcatccgt atctaatgct
      661 agatgatcaa tggctcctct acactcagta gttttatctt cccatatttc tattatcgtt
      721 ccattcaact cgaaaaagcc gactttactt cccacattca tttcttcatt aatcagacta
      781 aaccctaagg attcataaaa gatttttgaa gctccaaaat cttttgttgg aattccaata
      841 tgttgtaaac catttaattt cacttacatc atcccttcaa ttgttttaaa atccacattc
      901 ataatttatt gaatagtcta gtttatctac cttgattgct ccctccgtaa cagtcatact
      961 taatgctgtt ccgactttat catacattct agtacttaaa gcaacattat caatatataa
     1021 aacagctatc gtatcatcaa caattaactt tagttcatat tctttgccta tttccaaaaa
     1081 tagtggtcgt tgcagcccct tatcaaacat ttgaggccaa ggatagttcg gagtttttcc
     1141 gaaagtaagc tgattattaa tcaatgaaat acgaaattcg tatccttctt gagtatcact
     1201 atctacaaat aattttaaag caaactcctt tgttaacgat gtaatagtca aagttgtttc
     1261 aaatagaaaa taatc
//
LOCUS       DAZKLA010000252         1260 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000252 DAZKLA010000000
VERSION     DAZKLA010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.261"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(173..1030)
                     /locus_tag="ACG85N_002923"
     CDS             complement(173..1030)
                     /locus_tag="ACG85N_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0225600.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
BASE COUNT          359 a          253 c          183 g          465 t
ORIGIN      
        1 tctgtctttc aacattggag gaagttttta tttcttgaac ttggtgcact tctaattgct
       61 tattttcaga tggtgtaatt gcttgctgct ttttctttgc ttctgcttct ttttttattt
      121 tggcgagtcc tttgttgttc ccaaaaatat caagtccttt gatgtcagcc aattactcca
      181 cctccgcata ccaattctcg tgttcttcca attctttcaa aatactgata aataattcgt
      241 ggcttttgcg atcccattgt tttaagagac ctttctttgt cttctccatc gtaatacctg
      301 atccgtcgta tcgttttaag cgcgcatttt tgagtacaac atttttaatc aaatgttccc
      361 cataaatctc ttgggcttgt ttatacattt cctgatctac tgcgtcaccg ctttccaaca
      421 tgaaaggaat aacaccagca acatataaat ttgtatcaaa ttctttcaca aaaaagtcca
      481 tatactcaag atattgcatc gcatttctca tcgattttac ttgggtctgt aagacgacga
      541 ttgcccaatc cgcaatgtac atcgcggatt tactatatac agaaatagaa ggtgggacat
      601 ctaaatagat gacgtcaaac tcatcaaata atggttctag cttttctttc atatatttga
      661 attgggataa tgaatcatta tattggtctt tgatccaatc atcatacatt tcaaaggaca
      721 tgtctgctgg aagtatatag aggttattga ttactttata gacagcctct ttaggtggaa
      781 gattgtttat caacacgtca gtcacagtat tttcgtaaac aatcggttca ccttctccgc
      841 ctccttcttc gttagtgaag ttcatatgcg ttaagttcat cacatcagaa gtatttcctt
      901 gcatatccaa gtcaattatc aaggttcttt ttccttcatt aactgctgat tcaaacccta
      961 acatttgcgc gcacgtactt ttacctacgc cacctttaaa attaccgatt acatatttct
     1021 tcgctaccac gttcctacct cttttctttt agcaatattc atttgatact attataacat
     1081 atttattttt agttactacc aaaaaaaaga tatatatgta ctaccttata attaataagt
     1141 aaaaggtagt acataaataa ggaaaagctg aaatatattg atttatagct ttcctcaaag
     1201 taatgacgtt atttaattta tcgagaagca gagaatactc gcgactttag tcatgtagac
//
LOCUS       DAZKLA010000253         1251 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000253 DAZKLA010000000
VERSION     DAZKLA010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.262"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(856..>1251)
                     /locus_tag="ACG85N_002924"
     CDS             complement(856..>1251)
                     /locus_tag="ACG85N_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HFQ0225601.1"
                     /translation="SRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPA
                     FIAQGAHIDIRKNAVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTI
                     FELNTHRILNKNNKIVIGKHCWIGAKSVI"
BASE COUNT          375 a          258 c          141 g          477 t
ORIGIN      
        1 aatttaaaat taacattgat tctcctatga aaatatcttt tacctttctc ctgtgaaaag
       61 gaaatttcct tttttatagt gttattgata taatcagaat attcatcaaa ctctgtatct
      121 ggagttaaaa atctacagta acatctataa gctttgctag taatcttctt gggttctctc
      181 tccattgtca agcaacatca aaacacctac ttactattcg attcttttct gccaaaaacg
      241 agtcagcatc actcacaact tatgtgttgc tgactccgac tagtcctttg ttcaatatca
      301 acttattaaa cagatataat atttttcttt atttcgtata ctccaagact tccatctttc
      361 tttcatctta tctctgaaaa aaacacagca aagtgccctt aataaatata cattagaatt
      421 tgcaaatttt ttcatcttaa gatatatcct attcatattc tttacaatcc taatgggtga
      481 ttacaaatct atttagttca agttctacat tataaacacc tttcttgata gtatcatctg
      541 ctgcacaagc gatgtcgatc cacttattag gggatttacg tgctcaaaag tggctattcc
      601 ttttgggaag tccatccata ataatggtcc atggattttt ttagggtctt gtcgttcaaa
      661 tttctcaaaa actttattat acttccatag ttttataatt atttggtttc actttggctc
      721 gttcaaacac caacttgtat ttcttttcaa cgttcgaact ggagcccccc ctataatctc
      781 attattattt tcatgacttt ttactaccgt ggaattacaa gcaacaacaa tattatctgc
      841 taaatgaaca cctttctata tcacgctttt tgctccaatc caacaatgtt tacctataac
      901 tattttatta tttttattca aaatcctatg agtattcaat tcaaaaatcg tatgtgaatc
      961 ggtatccata aataatgtat cccacgatac tgtacagcca tctttgaatt gaatttcctt
     1021 aaagcaaatt atactcattt ttcctgtcga attaaacttt gacccaatat ctaaaactgc
     1081 atttttcctt atgtcaatat gagccccttg tgcgataaac gctggccctt gtattcttag
     1141 tattccttca atacttataa tcgtttcttc atttcccgca gtatttaata ctggaaagcc
     1201 caaataaact atttttccag attgtaatac tatctcaatt ttccctctcg a
//
LOCUS       DAZKLA010000254         1246 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000254 DAZKLA010000000
VERSION     DAZKLA010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.263"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1181
                     /locus_tag="ACG85N_002925"
     CDS             <1..1181
                     /locus_tag="ACG85N_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFQ0225602.1"
                     /translation="AQFIKGRLDDSPSHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTI
                     LELKRTRFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMELYENQSRKLIAKKYFVS
                     DGTVTRILREATKHYQPRMNFLPTVLCMDEFKSMKSVSGSMSFICVDGTTNQLFTILE
                     DRRLYKLTQYFLRFPRKARLKVKYLVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHIN
                     RSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTLEPLKRHYHRLFKRPIS
                     QTEIVDELLSYNEELRTAYHFCQLLRYYFVKRDTEGFQETLKTISSTLPQSFKKKFTI
                     FHRYQSGISNAFKVPHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN
                     "
BASE COUNT          382 a          271 c          244 g          349 t
ORIGIN      
        1 aagcgcagtt tattaaaggg agattggatg actcaccttc ccattgtgaa cactgtggat
       61 cgattcgtat tatccgcaac ggttcctata cgacacgcac tcagatgttg aaagtcaaag
      121 aaaagttgac tattttagaa ttaaaacgta cccgttttct ctgccatgat tgtggacgga
      181 ccttttctgc gaaaacggac ttagttgatg aacatcatca actaacaaag gaactgaagc
      241 aggcgattct catggaactg tacgaaaatc aatcacgtaa actaatcgct aagaagtact
      301 tcgtctcgga tgggacggtg acacggatct tgcgtgaagc aacgaagcat taccagccac
      361 gtatgaactt ccttccgacg gtgttgtgta tggatgagtt caaatcaatg aagtctgttt
      421 ctggttccat gagttttatt tgtgttgatg ggacaaccaa tcagctattt acgatccttg
      481 aagatcgcag actttacaaa ctaactcaat acttcctgcg ctttcctaga aaggcccgtt
      541 taaaggtcaa gtacctagtg atggacatga atgccagcta ttgtcagttg cttaaaacag
      601 tctttccaaa tgcggagatc gtaactgacc gctttcatat tgtccaacac atcaatcgca
      661 gcttcaatca attacgtgtc cagatcatga atcgttttcg aacctcacac tctgaaaatc
      721 agaagaagta ccgtcggctc aagcgatatt ggaaactctt actcaaagat tccacgacat
      781 tggagccact caaacggcat tatcatcgac tgtttaaacg accaattagt caaacagaaa
      841 tcgttgatga gctgttaagc tacaacgaag aattgcgaac tgcgtatcat ttttgtcaat
      901 tattacgcta ttatttcgtt aaacgagata cggaaggctt tcaagaaacc cttaaaacaa
      961 tttcatctac gttacctcag tcgtttaaaa agaaattcac gatcttccat cgctaccaat
     1021 cgggtatttc aaatgccttt aaggtgcccc actcaaacgg cgtcactgag gggctgaata
     1081 acaagatcaa gctgattaaa cgaattgcgt ttggttatcg caacttttat aactttcggg
     1141 cacggatcta tttacaacaa ggtctgatct ttgaaaacta aaaaaatatg cggcgcacag
     1201 aacgcgccgc atatcggact aagttttatt caaatttgtc ttcgcc
//
LOCUS       DAZKLA010000255         1231 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000255 DAZKLA010000000
VERSION     DAZKLA010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.264"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..180
                     /locus_tag="ACG85N_002926"
     CDS             61..180
                     /locus_tag="ACG85N_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0225603.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            complement(428..976)
                     /gene="aac(6')"
                     /locus_tag="ACG85N_002927"
     CDS             complement(428..976)
                     /gene="aac(6')"
                     /locus_tag="ACG85N_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0225604.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          379 a          244 c          182 g          426 t
ORIGIN      
        1 gattttttcg tgaggtttca aggaattttt cagaaaaaat agctttgtac aaggttttcc
       61 atggtcttcc aaaagccatt aaataatttt cgttatattg tctattattg ccatacaaag
      121 ataaaaaaga ataaacaaaa agaaactgtg acaaaagtct tgtcacagtc ccttatctga
      181 ataaacggtg ttcaaaagcc agtttctcgg tattaagcca aatttatgca aaaatgtaga
      241 aagccatttt cacaaaattt ttcttactac tcgaaactaa gcgacttttt cacaacctct
      301 ggtacacggt gttccaaaag tagcttcttc ggaaataagt tggaatttca caaaaattca
      361 agaagtaatt ttcgtaaatt ccttcttatt tcttgaagct gattacttct gtcacaacct
      421 ctttttatta ttgagaattt ggtcgaggaa taatcgtttt tgccatccaa atatccggtt
      481 tgtcccagcc atttgcattt ggtaagacac ctacgatttt ataacctaat ttttcataga
      541 attcatacgg atgttcacga aggttctgga tggaagccac tttatcaaat gtatgctcat
      601 acaggtccgt ttgacttaac gttgttccat ggtctaaatc atccgtacct aaataaatcg
      661 tgattcctcc tctggaagct acttcttttt ctaagtaatt gactaatcga gtacctattt
      721 ggttctttcg tcgggagctt tctacaacta atggatgcaa ttcccaacct gtgataccgt
      781 attgagggat tgcaccaata aatcctacta actcatcttg gtctaccgct gctaccgcga
      841 ttcgttctgg attcatcatt tcttctactt cttctgccga gctgtctcca tattcttccg
      901 gccaagtcag tctcagtaaa tcagaaagct gatctttcaa tactggatta ttacggtcaa
      961 attcactgat tatcatacgt cttccccctt gttcttgctt attctaacag tccttttgaa
     1021 aaaacgaaat gttctcgctc aaaaatcttt agctaccaat cagtatctct attctttcat
     1081 tatttatctt acttataaaa taaaaaaatt atacagaaac actttattca aaataggaaa
     1141 gatcaggaaa gccatcaatt ggaggaaaaa actctgtttg ttcatcatga tatagattat
     1201 cttttgcttt aaaaaaaacc aattatgaat t
//
LOCUS       DAZKLA010000256         1223 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000256 DAZKLA010000000
VERSION     DAZKLA010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.265"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..712
                     /locus_tag="ACG85N_002928"
     CDS             <1..712
                     /locus_tag="ACG85N_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFQ0225605.1"
                     /translation="TPFDEESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLL
                     STGMADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSD
                     HSLGYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLG
                     DGVKRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGK
                     KVKRPISADSVLLEEDVE"
     gene            709..>1223
                     /locus_tag="ACG85N_002929"
     CDS             709..>1223
                     /locus_tag="ACG85N_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFQ0225606.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVG"
BASE COUNT          405 a          162 c          267 g          389 t
ORIGIN      
        1 tactcctttt gatgaagaaa gtgcagactt tctaaaacaa attggtgtac aaggatttaa
       61 aattggctca ggagatttaa caaatttacc ttttcttaga aaaatagata tgtatgggtt
      121 gccagtttta ttatctacag gtatggctga tattcatgag gtcagtgagg ctgtagattg
      181 tttcatagat agtccagtta ctattttaca ttgtacttca aattatccgg ctagttctga
      241 agatattaat ttattggcga ttaacacatt aaaagaaatg ttcgggatac ccgttgggta
      301 ctccgatcat tcattgggat atgatgttgg tatttgtgct gttgcgatgg gtgctaaggt
      361 aattgagaaa cattttacct tggataagga actacctggt ccagatcata aagcttcttt
      421 agatcctcag gaatttgcag attttgtaaa gcatattaga aacactgaaa tatttttagg
      481 tgatggtgtt aagcgagcaa tgccttctga acttagtaca aaagaagtat caagaaaaag
      541 cctagtggtc actgagaatt taaatgtggg agatattctt acagagaaat ctcttacaat
      601 aaaacgtcct ggaaatggaa tagctcctaa gtttttatat caatatattg gcaaaaaagt
      661 taaaagacca ataagtgcag atagtgttct gcttgaggaa gatgttgaat gaaaaaaata
      721 cttatagtaa gtggaacacg agctgattat ggaatatata aacctgttgc tgagaattta
      781 ctacaagata attgggatgt atcatttgtt gtttctggaa tgcatttatc ccataaatat
      841 ggatttacta aagaattgat cttagctgac ggatttagaa ttagtggaga agttcaaagt
      901 ttgtttttag agaacaataa gggaaacatg gctcggtctg tggcattaga gataaatggt
      961 ttcacaaata tatttgaaca aactagccct gattttgtat tattactagg ggatagagga
     1021 gaaatgttgg cagctgcgat agcttgtttg tatcttggaa tcaaaacagc acatattcac
     1081 ggaggcgaag tgtctggtac tgtagatgaa tcaataagac atgccataac gaaaatagca
     1141 tctgttcatt ttgctgctac ggaaaaaagc aaagagcgtt taataaaaat gggggaagat
     1201 tcttggagag tttttcccgt cgg
//
LOCUS       DAZKLA010000257         1221 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000257 DAZKLA010000000
VERSION     DAZKLA010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.266"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            79..339
                     /locus_tag="ACG85N_002930"
     CDS             79..339
                     /locus_tag="ACG85N_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225607.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(701..940)
                     /locus_tag="ACG85N_002931"
     CDS             complement(701..940)
                     /locus_tag="ACG85N_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225608.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          431 a          167 c          223 g          400 t
ORIGIN      
        1 gtgttattga ttttcataat catattttaa tggtattatc attttaaata atggaaaaac
       61 attaaggatg gatgaaatat gaataacgaa actatcattt taggaaaaaa atatatttgc
      121 caacctattg gcttgaaaca aaaagtggtt ggagaagtaa tcagtaaatt agaaaattgt
      181 gttgtgatct gcgtagacga atatcatgac gtcgatcgca atgaaatatt agaaaaatgc
      241 ggaaaagtag tggcaaaata tactcacgtt tatgagttgg aaacaacaga atatctatat
      301 aactatagca acaaatatga acatacttta gtttcataaa gatttaaata aagtgccttc
      361 aaaagcgaga agaccaagct tttgaaggca ctttatttag ttggcagtga ttgttcatga
      421 tttttgattt attcaaaagg ttcgcctaga gaatctgtct ctgtaacgag gcctggtgga
      481 gaaactcgat aaaaacctat actgccagaa ctgtcctatt ttcggataga caaactagta
      541 gttttaaaaa gatcatccga tccaatcatt tcaaaaaatg caaaactgat atcaggatca
      601 tttagctcag gcttgttatc gtaaagcatt tgtaaaggtt gcatttagtt aggagtaaac
      661 gcgaaactat catcattatt tgctgaagaa gatgaagaag ctaattcaga gtttgtttga
      721 aataaaggag acgtgctgac agtttcttta ccctgttttt tgcagaggta gaaactttta
      781 tatcggaagt ttctaccgga gaatttttct catttgtaga aattgttgaa tcgatactat
      841 tcgaaatttg cgtatctgaa ggtatgttct ttgatgcaga agtcccacaa gcagtaagta
      901 acaataaatt tgataaactg ataaacagaa ttcttttcat tatgtacaca ctccttatag
      961 tatcgtttag catacaccta tgctcagtat taggcaaaat caatgcaaaa ctatcaagtc
     1021 ttttatagta tctattagtc ttagtaatac atatatggag agggtgatat cccagttcaa
     1081 accagataca ttggttacca tctgtattaa cgattatgcc tctaaacgct ttttttatga
     1141 aaaaatttat aagtgaaaac gtaggtatga tattatctag aggtaatttt tttgggtatt
     1201 taaatagttg ctaggaggat a
//
LOCUS       DAZKLA010000258         1219 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000258 DAZKLA010000000
VERSION     DAZKLA010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.267"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..972
                     /locus_tag="ACG85N_002932"
     CDS             19..972
                     /locus_tag="ACG85N_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0225609.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            944..1219
                     /gene="prgO"
                     /locus_tag="ACG85N_002933"
     CDS             944..1219
                     /gene="prgO"
                     /locus_tag="ACG85N_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFQ0225610.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
BASE COUNT          440 a          189 c          234 g          356 t
ORIGIN      
        1 aggaaattgg agggaactat ggaaaactta cggactgcat ttaaaacatc agataaacta
       61 ggaaaagttg taatgcttac aggtagaaaa ggcggcattg gtaagactac agacaatgat
      121 ttattagcga ttgtttctag tcaacttttt gaaaaagacg tgttgctaat tgattatgac
      181 caacaaagaa atactacttc caacattgga tcaacgtatc aaattactag ctttgatcga
      241 tccatgtctg cagcaatcaa aaaaggggac tgggtttcag gtattactca agtaagtcca
      301 catttatata ttatggctgg ctctcctgga tctgaagaac ttaatgaata cttgtccgaa
      361 aaatatcctg atcgtcgtaa gagatcattg gccttcatta aacctttaga agaattgaga
      421 aaaaatttcg attatatctt tatcgactgc ccaccatcaa cagataatgt ggttcgtgca
      481 ttcttaactg ctgcagatta tattattgca atgcaagaat taaaacggta cgcaatggaa
      541 ggaacagaag attttattaa taaagtttta gttccgattg tcacgaattt tgaagagtct
      601 catcttcaaa tcattggaat tttgcctgtt cttttctcag tgcgacgttc ctctcaacac
      661 gcaaactatc aaaaaacaat tgataagtat ggtgaatcaa acatttttaa aagcattgtt
      721 aaaggctcgg atcggttaga aatgtatggt gagaatggta tccaattaaa tgattatgta
      781 gatcgaagat ggtgggctat ttttgcagac atctttacag aattagaaga aagaattgaa
      841 tactatgaac aacatggtga tattgaagga tttgattata cacctaaata tgctgactca
      901 atggccaatc atatcctacc tcttggaaag gagattaaac taaatggcat tattaccaac
      961 gaacaaagat aagaagaaaa ttggtgctga agatatttca attaaagatg atattaatgt
     1021 cgagttaggg gaacgaaagt acactgcgaa agaaagaaaa ccagttcaag tagatcctcc
     1081 cgtactaaaa accattcggg atcttagtta tgcaaaagat attcctatgt atgaaatagt
     1141 caaacttgca atggacgcct atgttgatac tttaaacgac gaggaacgga ttatatataa
     1201 tagacgatca aaagattaa
//
LOCUS       DAZKLA010000259         1215 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000259 DAZKLA010000000
VERSION     DAZKLA010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.268"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            240..>1215
                     /locus_tag="ACG85N_002934"
     CDS             240..>1215
                     /locus_tag="ACG85N_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0225611.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTT"
BASE COUNT          486 a          239 c          220 g          270 t
ORIGIN      
        1 ttaggtcgaa agcgaaaaag aatcgttgtt atcttaagaa atgctagact taacgcggct
       61 ttttcatgat cattttctga aacgaacagt tttgtcattt ttaaaagttg ttttgaaaca
      121 tttttgtaac ataactatta tatcgttgac acactgctat ggtacaattt ttatatcgtt
      181 aagaaagact taaatatatt tagatattta ttaaattaag ccagtcggag gaatgaagag
      241 tgaaaaagag tttaatatca gcagtaatgg tatgttcaat gaccttgact gccgtagcat
      301 caccgatcgc tgccgcggcg gacgattttg attctcagat acaacagcaa gatcaaaaga
      361 ttgcagactt gaaaaatcaa caagcagatg cacaatcaca aatcgatgcc ttagaaagcc
      421 aagtttctga aatcaacaca caagcacaag acctattagc aaaacaagat acattgcgtc
      481 aagaatctgc acaattagta aaagacattg cagatttaca agaacgtatt gaaaaacgtg
      541 aagatacaat ccaaaaacaa gcacgcgaag cacaagtaag caacacaagc tcaaactaca
      601 tcgatgctgt attgaatgct gattcattag cagatgctat tggacgcgtt caagcaatga
      661 caacgatggt aaaagcaaac aacgacttga tggaacaaca aaaacaagat aaaaaagctg
      721 tagaagacaa aaaagctgaa aacgatgcta aactaaaaga attagctgaa aaccaagctg
      781 ctttggaatc tcaaaaaggc gatcttttat ctaaacaagc tgatttgaac gtactgaaaa
      841 cttcattagc tgctgaacaa gcaactgctg aagacaagaa agctgacttg aaccgtcaaa
      901 aagcagaagc tgaagcagag caagctcgta ttcgtgaaca acaacgtcta gcagaacaag
      961 ctcgtcaaca agctgcacaa gaaaaagctg aaaaagaagc acgcgaacaa gcagaggctg
     1021 aagcacaagc tactcaagct tcatctactg cacaaagctc agcaacagaa gaatcatcag
     1081 ctacacaatc ttcaatgact gaagaatcat catcagctac acaatcttca gcaacagaag
     1141 aatcaacaac acctgaatct tcaacagaag aatcaacagc gcctgaatct tcagcaacag
     1201 aagaatcaac aacag
//
LOCUS       DAZKLA010000260         1214 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000260 DAZKLA010000000
VERSION     DAZKLA010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.269"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1026
                     /locus_tag="ACG85N_002935"
     CDS             <1..1026
                     /locus_tag="ACG85N_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFQ0225612.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
     gene            complement(1080..>1214)
                     /locus_tag="ACG85N_002936"
     CDS             complement(1080..>1214)
                     /locus_tag="ACG85N_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0225613.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          427 a          179 c          207 g          401 t
ORIGIN      
        1 ttgacaggga atcctaaaaa cattcggctt tttttatata atctttttaa ctcaacagag
       61 ataagttttg tggaattgta tacgctacct ataattataa aaacagaaaa aatgttaaaa
      121 gatttaaaac agtattatcc tctgtctgct tatgaaaaaa agaaattatt ccacttttta
      181 gcaatcaacc tgttggctga acaagctgat tctttagata ttgctagcaa tcagtcgcta
      241 tttttttcgg atataggatt ttaccactta aaaggagtat taggagaaaa atccgttatt
      301 tttatcaata agacactctc ttggctttat cttcatgata aattggatca aaaatacttg
      361 agaaaaaatg aagatgaaac aatcgaatgg ttgaatgaac gattcattga aagttttgaa
      421 aatcagttta atctgttgcg agaagagacg agccaattga tccgaagaga gttaaccaaa
      481 atacatttta aacttttgca ttacactatt aataaagctg atgaatttcc acttgatgtt
      541 tcttttttta gagaaaacta tccagcattt tattgctatt tgatagagta tataaatgat
      601 ctaacgtcaa aacgcaaaga gttgcttcaa gataaatttt ttcttttttt cagttattta
      661 ttattattga tcaatcacat acccattcaa ctgatttctg aaccagtaaa gatcatcatc
      721 gatttttctt atggtgctgc ctataatcaa tttatcaaga aaaatttaag cttgtacatt
      781 aatctgaaca cggaagtgat agaggcagga aagcctgagt tcccggatgt tataatcacg
      841 aataggaata atctatatga agaaagtgca tctcaagtag tggtttggct cgatccgcca
      901 agagcagtag actgggggaa tcttacaaag attttgttga ctatccaaga agagaaatac
      961 aaagacagca aaattgacaa gcaaatagac gattttcctg aagaaatcat cgacaggatc
     1021 gaatagtgaa cttcttttag aaagtccgac tgctttttct aaaaatatta tttgtcaaat
     1081 taagcgagac aaaactttcc atttacgtaa ctcaaaaaaa cttctaatgg tgtttggtaa
     1141 tttagtgatt ttctagggat atggtttctt ttggacgcaa cggatgagat gaatccttga
     1201 ttgacttggt tgaa
//
LOCUS       DAZKLA010000261         1195 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000261 DAZKLA010000000
VERSION     DAZKLA010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.270"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..>1195
                     /locus_tag="ACG85N_002937"
     CDS             52..>1195
                     /locus_tag="ACG85N_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0225614.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQ"
BASE COUNT          365 a          234 c          237 g          359 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct
      361 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
      421 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
      481 tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag
      541 gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc
      601 atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat
      661 ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt
      721 attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg
      781 atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg
      841 aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa
      901 ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg
      961 gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta
     1021 gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta
     1081 aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca
     1141 gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaa
//
LOCUS       DAZKLA010000262         1171 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000262 DAZKLA010000000
VERSION     DAZKLA010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.271"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..691)
                     /locus_tag="ACG85N_002938"
     CDS             complement(<1..691)
                     /locus_tag="ACG85N_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HFQ0225615.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPPFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQ"
BASE COUNT          363 a          207 c          207 g          394 t
ORIGIN      
        1 attgtttttc tcgttcgcct ttgacgtagt agccgctttc cggatcagct ttacttattt
       61 ttctttcttt cagcttcact tccttttcta agtccaaagt aaaagggcgt tttccagcct
      121 ctattcgttg acgattgatc tcatcttcta attcatcctg ataggctttc gcttcatcat
      181 gggtcatttc tttcgtaaac ttatgtttat tggcgttagc tttgatgtgg gtagaatcca
      241 gatacaaatt atcctctgtt acaaaacctg ctttaactgc ttgttctaaa atataggcaa
      301 aaatatcctc aaacaccgtc gtttctctaa atcggcgaac ataattcttt ccaaaagttg
      361 agaaaggggg gattttctcc tcgaaggagt agcctaaaaa ccagcggtaa gcgacattgg
      421 tatcgacttc cttaatggtt tgtcgcatgg aacggatacc gaataaatac tgaataaaca
      481 ccaatttgat cagaatcact ggatcgatac ttgggcgacc taaatcggag taagtggctt
      541 cgacgattgg ataaataaat tcaaaatcaa gcgcggcatc aatctttcga acgagatgat
      601 cttgaggaac tagatcatca atcgtaagca tcgcaaattg atttctacca ttcgtattgt
      661 ttttagacat catagctatc actccttgca ttcgttttca taaatctatt ataaaagaaa
      721 agactgtaga catcatccat aaaatggact ttgtctacag tctggataac gtgatttttt
      781 gctgtaatga caaggtttta gctgtttcag ataccatgta tccaaaaata gtttattgag
      841 aaaataggag agtatttgat actaactagt aggaaaaaat gagataaaaa gtaaatggaa
      901 tcatttaagg taatctgaaa taaaaaatat acagccctgt ggtactttac ctacaaggct
      961 gtatatattt tttactctca gcgtttttga ctccgtgtct gactccgttt tattgatttt
     1021 gaatgttatt caatgaccat ttaacaggtc aaatttgatg tttttgattc cgtatgacgc
     1081 cgtttgatat ctagtgatat agtgatttga gtctacatcg aataagggaa taaaccaata
     1141 aaaaatgatt tagaatatta aaataacaaa a
//
LOCUS       DAZKLA010000263         1170 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000263 DAZKLA010000000
VERSION     DAZKLA010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.272"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..460
                     /locus_tag="ACG85N_002939"
     CDS             197..460
                     /locus_tag="ACG85N_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0225616.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            460..732
                     /locus_tag="ACG85N_002940"
     CDS             460..732
                     /locus_tag="ACG85N_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0225617.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            809..1138
                     /locus_tag="ACG85N_002941"
     CDS             809..1138
                     /locus_tag="ACG85N_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0225618.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          437 a          148 c          228 g          357 t
ORIGIN      
        1 tatgcgtttt tttgttgatt taccaacatg tttagacatt ttcctctagg aaaaaattaa
       61 ctagcaccac gggtataaag gaaacggata acactttaca atgctgtttt ggtactccaa
      121 tttaaacaaa acaatcgaat ttacgtactt aattaagtac gttagaatgg agatataaat
      181 tagaggggga gtagatatgg caaaaacagt cttaaatcct agctcagcac ggaaaaattt
      241 ttataagttg ttgaaagatg taaatgagaa tcatacggaa attgaaatca ttagtgagcg
      301 cagtgaaaat aatgcggtgc ttattggttt ggatgattgg aaagcaatca aagaaacgct
      361 attacttgag caaataggca ctttgggtgt tgttcgtaag cgagaaaaag atgatagtgg
      421 atttactaat gttgatgaaa ttgattggga taacttataa tgaataacta ttctgtgatg
      481 attaaaaact cggctaaagc tgatttaaag aaaatcaaac agtctaattt gaaacctcaa
      541 tttgagaagg ttattcagac tttaaaggaa gatccctatc tccctaccca atcatttgag
      601 aagttaaaac ctacacatga aggtaggtat tctagaagat tgaatagaca acaccgggtt
      661 gtttataagg tagatgaaga tgaacacgtg gttgaaattt attcagcaag gacgcattac
      721 gagtcacagt aattacttgt aaatacatta tctcgtggta taatgtattt actaagtaat
      781 tacatactgt tagaaatgag ggatttaaat gattacaaaa acaagaaaac aaggcaattc
      841 aatcatgttg acagtaccta aagattttaa tgttcctaat ggagtcgaag ttgaagcaaa
      901 gttggtagag aatggtattc tttatgaatt tgttgaacca caaaaagaat tttatgattt
      961 tagcgaagat attttgtctg atataattgc agaaggctac gataaagatg agattcttgt
     1021 agaatttaag aatagaaaaa acaaaatgca ttcttccttt agagatattg ctgaagatac
     1081 acttactaat tctaaagtaa tgactaaaga ggagttggca acagaaattg gcttatgaaa
     1141 tcgttccttc aaaacatgtc attaagtatt
//
LOCUS       DAZKLA010000264         1164 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000264 DAZKLA010000000
VERSION     DAZKLA010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.273"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            251..841
                     /locus_tag="ACG85N_002942"
     CDS             251..841
                     /locus_tag="ACG85N_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HFQ0225619.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            868..>1164
                     /locus_tag="ACG85N_002943"
     CDS             868..>1164
                     /locus_tag="ACG85N_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0225620.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNT"
BASE COUNT          419 a          130 c          236 g          379 t
ORIGIN      
        1 tacttgtact attgtaaaaa ttcgttaggt ttaaataact ggaaatatcg gaaaatgggc
       61 attatacatc acggacatcc cttcttttgg aaaaaatgtt acgattttta taagtaatat
      121 tgtatccggt ggaatcaacg atgaagataa catatccatc gttgtagatt cagtagcatt
      181 gaatgatgct cttgttgcaa gaaatccgaa taaaattgtt aagtataaat tgaattaata
      241 ggagatatga atggaaatta gcagtaaaga atatttaaaa gcggttcttc gtatggaaaa
      301 gaatctatat atagaatcta aaggacaaca cacaaaatta ctaaaaataa tgaaatcaca
      361 tccggactac aacgcttggc ggtatactcg tcgaatgaga attacatctt attattattc
      421 aaagagaaag aaaaatatca tttatgcgat tatgtatatt gtgtcttgca gaagaatgaa
      481 tagtttggga agaaaatgtg gtatagagtg tggtgaaaat gtatttgatg aaggtgtaag
      541 attttaccat acacaaggaa ttgttattaa tggtaacgcc ataataggaa aaaattgtta
      601 cttatatggg aataactgta ttggaaatga tggaatcgat cctaagtgtc ctgtaattgg
      661 aaataatgta cgtgtatgcg tgggtgcaaa gattattggt aatgtaaaaa tagctaacaa
      721 tgtagttgtt gcggcaggag ctattgttat caaagattgt ctagagaatg gtgcaattct
      781 tgcaggtgta ccggcaaaaa taatagggta tgttaattta acaaaagtat ttttagaata
      841 atttttagcg aaagaaggtg aataacgatg cgtatttcaa taaaaagatg gtcaggagca
      901 gattggacat tgttcacgtt ggcatttttt gcaatctcca tagttcttgt atatactttt
      961 aacgccccaa gagcaatttt atatttggga gatataatta atatctttgt gttattaaat
     1021 gcacttagct ttagacgcag aaaaggaata tatgctagca aaaatgagat gctgatagtt
     1081 ttgtttttac tagtttctat ttgtagctca gttattaatt tagaaaatat aggattgatt
     1141 atctggggga tacgaaataa cact
//
LOCUS       DAZKLA010000265         1137 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000265 DAZKLA010000000
VERSION     DAZKLA010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.275"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..355
                     /locus_tag="ACG85N_002944"
     CDS             <1..355
                     /locus_tag="ACG85N_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225621.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="ACG85N_002945"
                     /pseudo
     CDS             615..910
                     /locus_tag="ACG85N_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            985..>1137
                     /locus_tag="ACG85N_002946"
     CDS             985..>1137
                     /locus_tag="ACG85N_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0225622.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          431 a          135 c          237 g          334 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa gggttctgtt gcaaagtttt ccaaaaaatc tattttagtg taaaattgag
      961 aaaaaagaca gagaggacag agtaatgaat cattttaaag gcaaacaatt caaaaaagac
     1021 gtcattattg tcgctgttgg ttactacctg cgttacaatc taagctatcg tgaagttcag
     1081 gaattgttat atgatcgtgg aataaatgtt tgtcatacta cgatttatcg ttgggta
//
LOCUS       DAZKLA010000266         1112 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000266 DAZKLA010000000
VERSION     DAZKLA010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.276"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(267..971)
                     /locus_tag="ACG85N_002947"
     CDS             complement(267..971)
                     /locus_tag="ACG85N_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225623.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT          321 a          194 c          199 g          398 t
ORIGIN      
        1 cttttttctc tatcagaaaa tatgtttatt attagaattt tttatagtta aggttaggca
       61 tcttaagaaa tcaggcaact ccaagatatc ttcaggtaga agagaagtcg cctaacattg
      121 taggatcata ggtgatttgt tggacaggtc aagctgatat ttgagcctga tgttagaagg
      181 aatttctttt tacttgctgt agaaattatt atatgactaa aaaggctggg attttaaatc
      241 ccagcctttt atgacaaata gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt
      301 tcgatcaaag gaatcaaagt caatttgact ttgtatttcc gttcgagtgc tttttcgata
      361 acgaactcat tttcagtagt catagaaata gttgctaatt tacgaggttc atcagcattt
      421 ttttcattaa taactacttc atgttcattt tgttctgtgt aaaatttagg atactcagca
      481 acggcttttg aaaaatcagc aaaacttaaa gcggcattcg tatcagttga gcgtgtattt
      541 tcagttgtgt ccgcttttgg tttttccgtt ttttcttctg gcacactgac tgtttcttct
      601 gtaactggtg tcgcaacagc ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc
      661 cagtaagaca cgatttcaat aagaagattg tcacggaatc gttcgaaaat cccgttttct
      721 cctgaacgaa ttctctttaa tacagtattg aagttgtcat cgattggcac gttgatccgt
      781 gtatcttcat ttgttttccc agctaatccg atcgttaaga tttgacggtt tttgggatca
      841 agcatgactt gtaacggcaa accataacgt cgcaagctct cttcttctag agaacggaat
      901 aattgtcctc ttacaccatc caatgaagga cggaattgat ttaataattt ttgtttctgt
      961 tttttcttca tatgttctct ccttcgctca taccctttcg attataagaa aaaaacagcc
     1021 aaatgaaaag gagtaatgca aaaaaacttg cgtatacgca aattacaaca gaaaaatttg
     1081 cgtattttcc cgttttttga aagaaaaata aa
//
LOCUS       DAZKLA010000267         1073 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000267 DAZKLA010000000
VERSION     DAZKLA010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.277"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1073
                     /locus_tag="ACG85N_002948"
     rRNA            <1..>1073
                     /locus_tag="ACG85N_002948"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DAZKLA010000268         1069 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000268 DAZKLA010000000
VERSION     DAZKLA010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.278"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACG85N_002949"
     CDS             <1..969
                     /locus_tag="ACG85N_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFQ0225624.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          185 c          214 g          302 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccat
//
LOCUS       DAZKLA010000269         1022 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000269 DAZKLA010000000
VERSION     DAZKLA010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.279"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..284)
                     /locus_tag="ACG85N_002950"
                     /pseudo
     CDS             complement(60..284)
                     /locus_tag="ACG85N_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(838..924)
                     /locus_tag="ACG85N_002951"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="ACG85N_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          352 a          178 c          151 g          341 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 at
//
LOCUS       DAZKLA010000270         1005 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000270 DAZKLA010000000
VERSION     DAZKLA010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.280"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..624
                     /locus_tag="ACG85N_002952"
     CDS             85..624
                     /locus_tag="ACG85N_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225625.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT          366 a          129 c          166 g          344 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagta
//
LOCUS       DAZKLA010000271         1003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000271 DAZKLA010000000
VERSION     DAZKLA010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.282"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..1003
                     /locus_tag="ACG85N_002953"
                     /pseudo
     CDS             370..1003
                     /locus_tag="ACG85N_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          184 g          295 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta cct
//
LOCUS       DAZKLA010000272         1001 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000272 DAZKLA010000000
VERSION     DAZKLA010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.283"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..970
                     /locus_tag="ACG85N_002954"
     CDS             122..970
                     /locus_tag="ACG85N_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0225626.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT          341 a          166 c          197 g          297 t
ORIGIN      
        1 ggcaactcgt atgattacgc ttgtcgtttt tctattttcc aattttagtt tcatatctaa
       61 tcttacttat gatatagtta taaggtctgg aaaaattctt tgaaacgagg aaattaaaca
      121 catgaaactt gcagtagtta cagatagttc ggcatatctg ccggaacgta tcaagaacca
      181 tcccgactta ttcgttattc caatcccttt gattatggat gggaaaattt ataacgaagg
      241 aatcgatatc gaagcggatg aatattatgg acttttaaaa aacagtaaag attttcctac
      301 tacttcacaa cctgtcgtag gggaagtttt gggtttatat gaggaattga aagagaaagg
      361 gtatgacacg attttaagta tccatctttc ttcagggatt tctggctttg tcaacacatt
      421 atttgcgatg aaagatgaca tcagcggagt aacggtcatt ccttatgact ctaagatcac
      481 tagtatgccg atgggacaca tggtagaagc tgccttagac ttaaatgatg aaggtcagtc
      541 ccttgatgaa atacttgcac atatcgatcg aatccgagat aatacctatg cttatctgat
      601 tgtagatgat ttgaacaatt tagtacgagg aggacgtttg acaaatggtg cagcgctgat
      661 tggcagctta ttaaaaatca aaccgatcct gacattttct gaagggaaaa ttgttttatt
      721 cgaaaaaatc cgttcaagta aaaaagcatt tgctcgagct gaagaaatta ttggtagaag
      781 aaataacgaa atacaaccgc cagtcaaact ttacgtgata catgcaaata atttagaagt
      841 agcgattgcg gagaaagaga aattgcagaa aaaatatcca aatgcagaaa tcgaaattgg
      901 tcatttcgga ccagtcattg gtacacattt aggtgaaaaa gccatcggca ttgccatttc
      961 agcgcaataa ttccttttat tcaaaaaaca taccaaaaat a
//
LOCUS       DAZKLA010000273          988 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000273 DAZKLA010000000
VERSION     DAZKLA010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.284"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..988
                     /locus_tag="ACG85N_002955"
                     /pseudo
     CDS             1..988
                     /locus_tag="ACG85N_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          351 a          175 c          197 g          265 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag cgatcgtaac
      481 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
      541 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      601 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      661 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      721 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      781 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      841 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      901 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      961 atgtgctaac gctagcgagg cagtctaa
//
LOCUS       DAZKLA010000274          981 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000274 DAZKLA010000000
VERSION     DAZKLA010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.285"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          341 a          150 c          140 g          350 t
ORIGIN      
        1 ataaccaaca gcaaaaaata caatgaaaaa caaacttgga actaatgtat cattaataaa
       61 tgttgaaaaa ttcagcaact cattactgta tgattttagc agcccaacca acatataccc
      121 tataaaagct aataagatgg ttaggaatcc tttagaattt ttactcattc atagacactt
      181 ccttaaataa cttttattat agaaggtatt gtaaataata atattgacaa aaagtgactc
      241 aaaataaaaa aaaccttaca ttatactgga atgaactgta ttgatagtgt acctaaaagc
      301 tctactgaca taagatcgtg taatcggtaa agtaatttta ttaattatac gggatgtaat
      361 caattagaga ataaactaat cataaaaggt acttttacat tcataattgt cgctttgaga
      421 ttctactttg gttcactatc tgacccaatt gcacattttc gatcattaca agtattggtg
      481 tcgttagaac aactaaataa aaactagcgg tagatttttc agaatctatg ctaatttctg
      541 tatggatgtt ccagtgatgg caaatttctc ttttcattgt ttttcttgtc gcatgatatg
      601 cctttttgtg ttattcagtt aaactataga aattctttcc tagacagatc ttgatcccaa
      661 attttacgtt tttgagcatc gatatagttt aaaatgacat tactcatatc taaagagttt
      721 agatttttta ggactaaaac tgtcagttgt tctttttctt ctttctgtta aagtattgtc
      781 gcgacttttc gtatacgtta agatatgtcg tgaaaaaaca agttttgtga cacaaaaaaa
      841 cgaatgactt gaggaaatca cctcaatcat tcgtttaaaa tgctatatat tctttagttt
      901 aagatacgga aattaatgcc atcgaccaca ctagtagaat gaacgacatt tacttggcct
      961 ttttttaatt tcgttatgtt t
//
LOCUS       DAZKLA010000275          976 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000275 DAZKLA010000000
VERSION     DAZKLA010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.286"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..447
                     /locus_tag="ACG85N_002956"
     CDS             37..447
                     /locus_tag="ACG85N_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0225627.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            461..817
                     /locus_tag="ACG85N_002957"
     CDS             461..817
                     /locus_tag="ACG85N_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0225628.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          332 a          153 c          194 g          297 t
ORIGIN      
        1 tgtagaattg gattataatt aaaaaggagt gagaaaatga taaaagtata tacttcgcct
       61 agctgtactt cctgtagaaa agcaagagca tggttgcaag cgaatcaact tgaatttgaa
      121 gagaagaata tttttgccga accgttaaca gaaagtgaga tcaaaaagat cttggcagcg
      181 actgaaggag gagttcaaga tattatttcc actcggtcga aaatttacga aaagctgggc
      241 atagatttta acgaattaac tttaaagcaa atgatcgtct tattcaaaga atatccttca
      301 ttattgagac gacctatttt gattgatgaa aaaagaatac taatcggatt taatgaagat
      361 gagatccggt catttgtccc tcgtgaaatt cgatgtgttg ccagaaatct gatctattta
      421 caaaacaata acatcttaaa ttcataggga gggagccttc gtggaagtga aagtctatgg
      481 atcagcgcac tgtgtaacaa gtctgaaggc gaaacaatgg ttagaacacc atcaattgcc
      541 ttatcaattt gttgacttag aagaacgcga tctaagtaca gaagaagcag aagaattatg
      601 cagttttgaa gaagtgcaag cagaacagct atttgcgact tggtctgagg cttttcacaa
      661 cattgcgatt gacctttcct gtgtaggaaa aaaacaactg atttttttat gcacacaaca
      721 taccaatctc ctcagacgtc ctttgattat tatcgatgaa gttctattta ttggatacaa
      781 tgagcagttg atggaacagc ttatcttaaa taaatagact ttaactgttt taatcaagtc
      841 actgagcatt tcatacagtt gtaacagtcg aacacttagc atcaagcgtg ttatgtgttc
      901 tttttttgac tttatttttc tactatagag acatgctcaa tttatgtggt caaaattcaa
      961 atctaggtgg ggatat
//
LOCUS       DAZKLA010000276          961 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000276 DAZKLA010000000
VERSION     DAZKLA010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.287"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..459
                     /locus_tag="ACG85N_002958"
     CDS             <1..459
                     /locus_tag="ACG85N_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225629.1"
                     /translation="AKYLKTHVEAIDGKEALVQTDGDQAIAGHKNFSGSVTINNKRVL
                     TTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGNDSVILTVPEGYR
                     TPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
     gene            473..904
                     /locus_tag="ACG85N_002959"
     CDS             473..904
                     /locus_tag="ACG85N_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225630.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
BASE COUNT          362 a          140 c          203 g          256 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 a
//
LOCUS       DAZKLA010000277          947 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000277 DAZKLA010000000
VERSION     DAZKLA010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.288"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            324..737
                     /locus_tag="ACG85N_002960"
     CDS             324..737
                     /locus_tag="ACG85N_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0225631.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
BASE COUNT          335 a          146 c          199 g          267 t
ORIGIN      
        1 ctataggtaa aaaagataac atgaggagct tatttgttct agagtgtttg acaaagtaac
       61 aaaatgcgac gataccaata caaacgggaa tccaaaataa cagcatgctc cacatagtca
      121 ctcaaccatc tcctatacgc tttattatca atagataata tcatttttcg taataaagat
      181 aaaaaaataa gtaaggcttc tcattaaaat ataaaaaaga cagccgacca ctgactgcct
      241 atataagagt attgaaataa aaagctgctg atataataaa ttccacaagt ttattatatc
      301 acataaagga gcggtttgac ttgatgcaat tgttacgaga ggtagatttc aaacagacaa
      361 gatgtaatgc gagagatgtg ctgaagaact ttcggcgttt ggagcggatg gcaggtcgct
      421 ctttgataga tattaagtcg ccgattatta cggatatgcc gaaggcgccg aagcacggca
      481 ataaggcaga agacgcaatc attcagatga tggatataga agcggagaga gacgcgattc
      541 tagcagcctt gatggctctt agtctgatta gtcgtcagat actctactat agcttttgtg
      601 tgccagatag cttctcaaac tacagaatta gccgtgaagt gggttattca gaaagaagta
      661 tacaacggat gaagtcggaa gctctaatag agtttgcaga agcatataaa cacggaagaa
      721 taattgctta taaataattt ggcggctttt tggcggaatg atggcggttt ttagctattt
      781 accagtgata ttatggtagt gtcgaaagat tagtgatagg tctaagacaa aataataata
      841 aaaggaacat cgttttatta ttgtttcaca attaagcttc gatagacagc aacggaaata
      901 ttaagaataa ggatgtgaat tttaactcct tctaaattgt tcttatt
//
LOCUS       DAZKLA010000278          929 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000278 DAZKLA010000000
VERSION     DAZKLA010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.289"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..325
                     /locus_tag="ACG85N_002961"
                     /pseudo
     CDS             23..325
                     /locus_tag="ACG85N_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
     gene            433..>929
                     /locus_tag="ACG85N_002962"
     CDS             433..>929
                     /locus_tag="ACG85N_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFQ0225632.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          347 a          140 c          159 g          283 t
ORIGIN      
        1 aaatataaga aggagtataa aaatgaacaa acataataga aaaataacga ataacaaaga
       61 actgatcttg ttactaatag ggatttgggg tatcttttta ctctttaata tgtatatgcc
      121 gactatgaga gcggatgacg tagtttatgc aagtagatta gatgaacttg gctatttggg
      181 tgcttcgata gaacattata agacatggag ttcaagaatt attatagaac tattcttaat
      241 gttcttttct aaacacttta tgctatggaa attattaaat tctacgatta tgttaggaat
      301 tgtagtatta ctttgcaagt atgttgagag tataaaatat tttgtgtaaa tgaaaaaatc
      361 catacaaaaa aggaagtccc ttctgtagaa taaagttaac gacaaccaat tcacagaaaa
      421 gaggacttcc ctatgaatga ttttactaca gaaattgtgc aaactctagt cactaaaggc
      481 gatttaaatg aattattccg ttcgcactta gaaaaagcga taaacacact cctacggact
      541 gaattaacgg cttttttaga ttacgaaaaa tatgatcgca ctggttttaa ttcaggtaat
      601 tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat atggtgaatt aacattggaa
      661 atacctagag atcgtaatgg tgagtttaaa caacaaactt taccagccta caaaagaaca
      721 aacgatacat tggaaaccac tattatccat ttattcgaaa aaggtgttac gatgtctgaa
      781 attgctgatt tgatcgaaaa aatgtacggt catcactata ctccacaaac catgtccaac
      841 atgactaaag ttctgactga agaagtaaat gcctttaaat ccagagcctt aaatgataag
      901 tatgtcgcta tttttatgga cgctactta
//
LOCUS       DAZKLA010000279          919 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000279 DAZKLA010000000
VERSION     DAZKLA010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.290"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..718
                     /locus_tag="ACG85N_002963"
     CDS             <1..718
                     /locus_tag="ACG85N_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFQ0225633.1"
                     /translation="IKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLS
                     NGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLP
                     EVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSET
                     KLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKY
                     AGVSPKQYQLGLVDNFNNIS"
     gene            735..>919
                     /locus_tag="ACG85N_002964"
     CDS             735..>919
                     /locus_tag="ACG85N_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225634.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCE"
BASE COUNT          341 a          101 c          164 g          313 t
ORIGIN      
        1 aatcaaaata gataatctgt tatcacaaaa ttacgatcca gaaatatatc tgtttcttta
       61 tgaaaataaa atactggaat atgttaaaaa tggagatatt cgaaatttag aaaacatggt
      121 ttttaatctg agtaatggaa taattccaag tgttagtgga gatacgattc ggtctgaaaa
      181 aaattattct atcattgttt ttgaaaagtt agcacaaacg tctataacat taggaatgga
      241 tataatagaa gcgtatcaaa gtagagatgc tttgatacaa gaaaacgaat tagctgtatc
      301 gttacctgag gtcttaaaag tgagggattc tgggattgtt tattatacta aggaaatcgg
      361 aaaaactaag atagagcatc tttctccctt aatttcttct gtcgttcaat tcatcggctt
      421 aaatatctat aaaagaatta ctgtaaagga aatagctaat tatttttctg taagtgaaac
      481 taaattaaga gaaagcttta agaatgaaat gcatattacg atttataatt atatctctaa
      541 gcgaaaaatt tctacggcta aaataatgtt gaaatcaaac catactatca gtgaagtttc
      601 tttaggttta ggattttcag attcttcaca cttttcgaga gtattcaaaa agtacgcagg
      661 tgtatctcct aagcaatatc aattaggttt agtggacaat ttcaacaaca ttagctgaga
      721 ggttttattt tattatgaga ctatatcatt tacgtagttt ggaggagttg aggaaattgg
      781 attttaacaa aaaatttttt tgggaaattt ctgatttgag ttgtttcgta gaatctgagc
      841 gtctaagtaa tggtaataat agtgtgttaa atgaatatga caaagtagta attaataaag
      901 taaaaagttt atgtgaaga
//
LOCUS       DAZKLA010000280          915 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000280 DAZKLA010000000
VERSION     DAZKLA010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.291"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..>915)
                     /locus_tag="ACG85N_002965"
     CDS             complement(109..>915)
                     /locus_tag="ACG85N_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFQ0225635.1"
                     /translation="TYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGL
                     QLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDL
                     TYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDR
                     GKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLR
                     LELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          245 a          212 c          177 g          281 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccgattcggc gccgactgag
      781 ttgaagccca cgctttgcta aacacttctt caattttcgg gtaccgtaag cctttcggtt
      841 tcgaataaac tcttcctgaa cgatctcttc taagtcggac tcattttcga tcggtttcgc
      901 ttgataatag taggt
//
LOCUS       DAZKLA010000281          899 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000281 DAZKLA010000000
VERSION     DAZKLA010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.292"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            256..879
                     /locus_tag="ACG85N_002966"
     CDS             256..879
                     /locus_tag="ACG85N_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0225636.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          333 a          129 c          167 g          270 t
ORIGIN      
        1 aataaactaa tgtccttatt tttgtgttca catttctgtt acctaagaaa tacttaaagg
       61 aggtgatgta gccagcaacg actacaactt gaacccgcta tctacagttt atttgttgtt
      121 tcttttgggt atactctaat ggtacaaatc catatttgct taaagcgttg atttttcagc
      181 ggtttttctt tattgcaaag tgtaccctaa gacctgtata ctaatagtaa atagattgga
      241 taggaatgat ggcaaatgta tttagcttat ttaagagttt cttccgacga tcaaagttta
      301 gctcggcaac ataagctaat tgaaaattgg tcagtcaaaa ataatattgc tgaagacgat
      361 ttaaaaattt tcgaggaaaa agtctctgga aaaaatattg aaaacagacc agagctgcaa
      421 gagcttattt ctttcatacg tgaacgagat acgattatta ttcctagtct tgatcgatta
      481 gcaagaaata gtaaagatac aaaagactta cttcaattgt ttcgtagtaa aggtgcttct
      541 attgaaatat tagacctacc gtcgtttaat ggggtaactg accctgcatt aagagacttg
      601 ctaacaaatt tagtcataga agtgtttagc tatgttgctg aaaacgaacg gaaaaaaatc
      661 aaagagcgtc aaaaacaagg cattgaaatt gcaaagaaaa atggtcgtta tactggtaaa
      721 aagattcaat accatgccaa tgcaacagga aaaaacaaat tgatttacga tgaaattgtt
      781 gatggtctga agaaaaaaga aactatttct aaaatagcaa agaacgcagg agttacaaga
      841 aaaaccgttt atcgagtaaa aagagattta ggaatgtaat atgttccagt tagaaagaa
//
LOCUS       DAZKLA010000282          897 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000282 DAZKLA010000000
VERSION     DAZKLA010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.293"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..>897
                     /gene="bsh"
                     /locus_tag="ACG85N_002967"
     CDS             4..>897
                     /gene="bsh"
                     /locus_tag="ACG85N_002967"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0225637.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAID"
BASE COUNT          303 a          122 c          164 g          308 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgat
//
LOCUS       DAZKLA010000283          822 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000283 DAZKLA010000000
VERSION     DAZKLA010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.294"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..153)
                     /locus_tag="ACG85N_002968"
     CDS             complement(<1..153)
                     /locus_tag="ACG85N_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0225638.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTS"
     gene            231..809
                     /locus_tag="ACG85N_002969"
                     /pseudo
     CDS             231..809
                     /locus_tag="ACG85N_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
BASE COUNT          259 a          151 c          158 g          254 t
ORIGIN      
        1 actcgtttta gattgcttta cccatttgtc aaatgctgaa gccgttagtt catattctcg
       61 aatgatttct acacgtggct ttccagctaa gtaaagattg acgatttgtt gcttgaattc
      121 ttgtgaaaaa gttcttcgtg ttctcttaga cataaaaatt cctcctggta tgttttcttc
      181 tagtctacac accttaattt ttctgtctag ttaattgtag cctatccaca ggaatcaaat
      241 cgcatttatg ggtaacacaa aatatttggg gcgatcatac agatccttgc ggttatcttt
      301 ctgaaatggg tgtaagtaaa gaaaaactag cctatgattt ggctcatggt tttacggatg
      361 ataatccaac tacttcggat gataatccaa ctacttcgga ggataaacca gtaattgatc
      421 caactcgagc aggtgctgca aattctacgc tgacagatgg aacaaattac gcccacgttg
      481 atcagttcgg agaaatcgaa aacgcaaact tgcatgtagc tggatggcac attgctaatt
      541 ataaatacga gtatattttc attatggact acaatactgg aaaagaacta gctagagtaa
      601 gagctgatgg gatttataga ccagacgtaa atcaagctta taatacttta ggaaatgttg
      661 gctatcatgt atctttcaat atgcgtgatt tccctaataa gaaagtatac gtcatgatgc
      721 gtgcgacaaa cgatccagaa ggaaatacta agggtggagc acaagatttt catgacaagc
      781 gttggtattt aaatattcca caacgataaa aaattccccc ct
//
LOCUS       DAZKLA010000284          816 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000284 DAZKLA010000000
VERSION     DAZKLA010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.295"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>816)
                     /locus_tag="ACG85N_002970"
     rRNA            complement(<1..>816)
                     /locus_tag="ACG85N_002970"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          165 a          259 c          181 g          211 t
ORIGIN      
        1 ctctagagtg gtcaaaggat gtcaagacct ggtaaggttc ttcgcgttgc ttcgaattaa
       61 accacatgct ccaccgcttg tgcgggcccc cgtcaattcc tttgagtttc aaccttgcgg
      121 tcgtactccc caggcggagt gcttaatgcg ttagctgcag cactgaaggg cggaaaccct
      181 ccaacactta gcactcatcg tttacggcgt ggactaccag ggtatctaat cctgtttgct
      241 ccccacgctt tcgagcctca gcgtcagtta cagaccagag agccgccttc gccactggtg
      301 ttcctccata tatctacgca tttcaccgct acacatggaa ttccactctc ctcttctgca
      361 ctcaagtctc ccagtttcca atgaccctcc ccggttgagc cgggggcttt cacatcagac
      421 ttaagaaacc gcctgcgctc gctttacgcc caataaatcc ggacaacgct tgccacctac
      481 gtattaccgc ggctgctggc acgtagttag ccgtggcttt ctggttagat accgtcaagg
      541 gatgaacagt tactctcatc cttgttcttc tctaacaaca gagttttacg atccgaaaac
      601 cttcttcact cacgcggcgt tgctcggtca gactttcgtc cattgccgaa gattccctac
      661 tgctgcctcc cgtaggagtt tgggccgtgt ctcagtccca atgtggccga tcaccctctc
      721 aggtcggcta tgcatcgtgg ccttggtgag ccgttacctc accaactagc taatgcaccg
      781 cgggtccatc catcagcgac acccgaaagc gccttt
//
LOCUS       DAZKLA010000285          816 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000285 DAZKLA010000000
VERSION     DAZKLA010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.296"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>816
                     /locus_tag="ACG85N_002971"
     CDS             <1..>816
                     /locus_tag="ACG85N_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0225639.1"
                     /translation="NIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAGLNNLFTSIFQM
                     LNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVFTLGLLCIPFLR
                     FLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVINVSMLITFTFQ
                     FVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVGELEKETIDDII
                     KKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIMSIFAII"
BASE COUNT          263 a           97 c          136 g          320 t
ORIGIN      
        1 gaatataatt actggaacaa tttttaagtt atatcagatt atcattccct tttttatgcg
       61 tacacttatg atttatcaga tgggaatgga gtatgctgga ttgaacaacc tgttcacatc
      121 tatatttcaa atgttaaatt tagcggaatt gggaataggt gctgcactaa ctgtaagtat
      181 gtataaacct atagcagaag acaatagaaa agagatttgt gctttactga acgcatataa
      241 aaagtacttt tcaattattg gatggatagt gtttacatta ggattgttat gcattccttt
      301 tttgagattt ttagttaaag gttcgattcc tggaaattta aatatgacag ttttgtattt
      361 aatgtatttg ataaatactg ttttgtcgta ttggttattt tcatatagaa agagtctaat
      421 atatgcacat caaagaaatg atgtaattaa cgtgtctatg ctaattactt ttacattcca
      481 atttgtaatg caggcatatg ttctgatttt cttaaaaaat tactatttgt atttattggc
      541 ttctattttg ggacaaatag ttcttaactt aatgtgtgct gttgtttctc aaaagatgta
      601 tcctgattat actcctgttg gtgagttaga gaaagaaact attgacgata ttataaaaaa
      661 agtaaaagga ttagttacaa acaaggtagg cggtacaata ttacgatctt cagattcaat
      721 tattatttca tctttcttag gtctttcagt acttgcgatt tatcagaatt attactttat
      781 tatttctgcc ataatgagta tatttgcaat aattta
//
LOCUS       DAZKLA010000286          763 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000286 DAZKLA010000000
VERSION     DAZKLA010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.298"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            9..722
                     /locus_tag="ACG85N_002972"
     CDS             9..722
                     /locus_tag="ACG85N_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225640.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT          250 a          150 c          134 g          229 t
ORIGIN      
        1 tattttatat gaaaaagtta ggttttattt tattgttggt ctctttattc cttgtaggat
       61 gtacgtctcc tagtaaagaa aatagtgatc catctgcgac cacagacaaa agttcaatca
      121 gacaaaccag ttcagctgaa aaatcaagta ctcagaaatc agcttctact gaaacgaata
      181 catcttcaac aagcagtaaa gtctcagagc ccaatacttc ttctacatct agtagtacag
      241 ttgagacaaa tatttcttct tcaacaatag acacaacctc ctcacagtct tctcagtcaa
      301 tagatccgtt agcaggatat tctgatctac aaattgagta cgcacgtgtt tggctagcta
      361 ttagaggtaa tacttttaga gatacatcgg gtgatttcga attacatgtt attcattatc
      421 ctgcaggaac acctatcgcc ccttatgatg cagggagtgc agtctatccg aaagatgtgg
      481 ttatgctcac tgggaaatac agttaccagg gagttttagt gtattcaagt aaccatgacg
      541 ggacaatcac tagttatcca gttccttcac attggcaaat gcctgcagat caaacatctt
      601 atccagcgtt cattagagaa acaactcaag aaatcattga taatgcatct gtagtagcta
      661 ttcctacagg aaatcccaat gacgtgaaac agttaatcga tgtgacagtt atagataatt
      721 agactttcta atatgttata caaaaagaag ctgacagcat ttt
//
LOCUS       DAZKLA010000287          761 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000287 DAZKLA010000000
VERSION     DAZKLA010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.299"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..649)
                     /locus_tag="ACG85N_002973"
     CDS             complement(<1..649)
                     /locus_tag="ACG85N_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0225641.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFI"
BASE COUNT          252 a          146 c           86 g          277 t
ORIGIN      
        1 taataaaact tgttattttt gaatgttgca aatatgaaat atggaatatg atatcttcag
       61 atatataatc cctttctgac ggaaacttga tattgtgctg ttgaattatc tcagatgaat
      121 acagcgcatg ccaaactgac attggcaata taaaatcgac tttataactc ggctcagtgc
      181 caatcattcc aagcaacact ttttcaatta tagcgttatt tgtaaatatc gtactatcat
      241 atcttgctgg ctttgaaata atattattat catcataata ttcataatat ccagcataac
      301 atgtgtctgc attactcctt tttataccat ttaacattgc ttcaatataa ttagctgcaa
      361 caaaatcatc agaatcaata aaagcaacat attttccttt tacatgttca agtcctgtat
      421 ttcttgccaa tcccaagcca gcattatttt tatgaataat acgaatcctt gaatccttta
      481 atgcatagtc ttcacatatt ttattagatg aatccttaga tccatcatcc acaattataa
      541 tatctaggtt cttatatgtt tgattacaca ctgattccaa acaacttcct acatactttt
      601 caacattata tacaggtatg attacactaa ttagatcact cgaattcatc cttctatcct
      661 ttcactcacg ttttctcaat tagtcaatga cggcatcaac gattttctta aattctctga
      721 aaaaaataga ctctgatctt gatgatgtga atgacatact a
//
LOCUS       DAZKLA010000288          759 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000288 DAZKLA010000000
VERSION     DAZKLA010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.300"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..385
                     /locus_tag="ACG85N_002974"
     CDS             2..385
                     /locus_tag="ACG85N_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225642.1"
                     /translation="MSEDVESQQFGTNVINKVINPALSINAPLIPTALSFSVTAVVSE
                     IEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHYA
                     VVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            399..>759
                     /locus_tag="ACG85N_002975"
     CDS             399..>759
                     /locus_tag="ACG85N_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225643.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDY"
BASE COUNT          276 a          104 c          136 g          243 t
ORIGIN      
        1 aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata aagtaattaa
       61 tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt ctgtaacagc
      121 tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg aagttttaaa
      181 caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc ctcctcaagt
      241 taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag caggagagca
      301 ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg atatcaaggt
      361 caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata gtaccatcaa
      421 atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc gcatcggtaa
      481 ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa aatgataatc
      541 aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat ggcaaaattt
      601 ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa tatgatgaag
      661 atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc tcagattacg
      721 atgttttgga tactctcgat aatgtattat ctgactata
//
LOCUS       DAZKLA010000289          750 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000289 DAZKLA010000000
VERSION     DAZKLA010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.301"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..291)
                     /locus_tag="ACG85N_002976"
     CDS             complement(<1..291)
                     /locus_tag="ACG85N_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225644.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEK"
     gene            complement(298..>750)
                     /locus_tag="ACG85N_002977"
     CDS             complement(298..>750)
                     /locus_tag="ACG85N_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFQ0225645.1"
                     /translation="KVNTVDLEIKNKLVDELTFYFDTVGFLDEHGVYHIKDFGAIRER
                     ISQDNEQGRAARVFYEHIIVPRMEKVDWKRYAGCVAINSLPGGGVAWAIGTQGDFIEA
                     LKAFNFEKAADLLLKIAEKQLGKAAAKELAKVNIVAGIAAAAASCALE"
BASE COUNT          242 a          154 c           81 g          273 t
ORIGIN      
        1 tttttctagt gaaaacttgg taatctcttt gtaattaatt cttgttaaag aataattaaa
       61 cacttcattt ttacttgtta ttttttcatt gactataact agttcatctg gtaaaaaaat
      121 taaccaacaa acatcgacaa acagtaaatg atataatcca tttccacctc caagcatatt
      181 caatactggt gaacatgcct tagaaacacg aatatagtta tggtttaaat agatattttc
      241 tttaaacatt gcttgtttaa aagccatatg ctcttgttct aaacttttca taaactttta
      301 ctctaatgca cagctagcag cagctgctgc aattcctgct acaatattta ccttagctaa
      361 ctctttagct gcagcttttc ctaattgttt ttctgcaatt tttaataata aatccgcagc
      421 tttttcaaaa ttaaaagctt ttaatgcttc aataaaatct ccctgcgtac ctatagccca
      481 agccactcct cctccaggaa gagaattaat ggctacacat ccagcatatc gtttccaatc
      541 aactttctcc attctaggaa caataatatg ttcataaaag actcttgctg ctcttccttg
      601 ttcgttatct tgactgattc gttcacgaat agcaccaaaa tcctttatat ggtatacacc
      661 atgttcgtct aaaaaaccaa ctgtatcaaa ataaaaagtt aattcatcta ctaatttatt
      721 ctttatttct aaatcaactg tattcacttt
//
LOCUS       DAZKLA010000290          728 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000290 DAZKLA010000000
VERSION     DAZKLA010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.302"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..>728
                     /locus_tag="ACG85N_002978"
     CDS             19..>728
                     /locus_tag="ACG85N_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0225646.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLA"
BASE COUNT          271 a           97 c          142 g          218 t
ORIGIN      
        1 tcataggagg agatataagt gtttaaagga aaaacaatat tagtaactgg cggaactggt
       61 tccattggaa gtgaaattgt tcgtcagctt ttaaaatatg aaccaaaagc tatcagaatt
      121 tatagtagag gtgaagaaaa acaattttat atgcaacaag agctctcaaa tcacactaat
      181 ttgagatttt taattggtga tatcagagac tctcaaagga ttgattatgc atgtcgtgat
      241 gtggatatta tttttcatgc tgccgcaatg aaacacgttc cagcaagtga atataatcct
      301 atggaagctg taaaaacaaa tgtgatggga actcaaaatg taatagatgc agcgatcaaa
      361 aataatgtaa aacattttgt aggtatcagt acagataaag tagtgagtcc tactaacacc
      421 atgggtgcga caaagttatt gagtgaaaaa ttaataatag ctgccaatca gtataaagga
      481 aaaacagaaa caatattttc ttgtgtaagg tttggaaatg tcatggggtc aagtggatct
      541 gttattcctc tttttagtaa acaaataaaa aacaataaag atattacttt gacagatgtt
      601 gatatgacac gatttatgat gtcaataccg gatgctgcaa gattagttct gtctgctgct
      661 atgaattcaa aatttggaga aacatatgtc ttaaaaatgc cggcaatcaa tattttaact
      721 ttagctca
//
LOCUS       DAZKLA010000291          725 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000291 DAZKLA010000000
VERSION     DAZKLA010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.303"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>725
                     /locus_tag="ACG85N_002979"
     CDS             <1..>725
                     /locus_tag="ACG85N_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFQ0225647.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGF
                     KACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESL
                     IGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGW
                     KVIQNPRYDQLVVGAIINWARGG"
BASE COUNT          226 a          112 c          179 g          208 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggaca
//
LOCUS       DAZKLA010000292          688 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000292 DAZKLA010000000
VERSION     DAZKLA010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.304"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..>688)
                     /locus_tag="ACG85N_002980"
     CDS             complement(14..>688)
                     /locus_tag="ACG85N_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225648.1"
                     /translation="YKNLNDLSRSISEYLKGNSYLKIRFSHILILLPYLNENFTLIQV
                     DKSFIASGNQGNMNITSNKTSRVLLALMGYGILYRDDQNGQILINRAGRSLLMKYRKF
                     IPEDFQNLQLENMRLEDLFRLAEDDKYRFEVLKIQKNELLNKLYPENAIKAHFGDANH
                     MYSWFIPKRFRGKINAGDQVKVLNRNSTDVVMITDIYVEDEDIIKYMKPVIKLVNKNK
                     GTRGAS"
BASE COUNT          216 a          129 c           81 g          262 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg ttcctttatt tttattaaca agttttatca
       61 caggcttcat atatttgatt atatcttcat cttctacata gatatctgtt atcattacga
      121 catctgtact atttcgattg agtactttca cttgatctcc agcattgatt ttccctctaa
      181 atcttttagg aatgaaccaa ctatacatat gattagcatc accaaaatgt gcttttatcg
      241 cattctcagg atataactta ttcaatagtt cattcttttg aattttcaaa acttcaaatc
      301 tatacttatc atcttctgct aatctaaata aatcttctaa acgcatgttt tctaactgta
      361 agttttgaaa atcttccggt ataaattttc tatatttcat taataatgaa cgcccagccc
      421 gatttattag aatctggcca ttttggtcat ctctataaag aattccgtaa cccatcaacg
      481 ccaatagcac acgtgacgtt ttgttacttg taatattcat atttccttga ttacctgagg
      541 caatgaaaga cttatctact tgaattaggg tgaaattctc attcaaatat ggtagtaaaa
      601 ttaatatatg tgaaaatcga atcttgagat agctattccc cttgagatat tcactgatac
      661 ttctactcaa atcatttaaa tttttata
//
LOCUS       DAZKLA010000293          688 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000293 DAZKLA010000000
VERSION     DAZKLA010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.305"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            31..267
                     /locus_tag="ACG85N_002981"
     CDS             31..267
                     /locus_tag="ACG85N_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0225649.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            264..662
                     /locus_tag="ACG85N_002982"
     CDS             264..662
                     /locus_tag="ACG85N_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0225650.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT          248 a          110 c          125 g          205 t
ORIGIN      
        1 gaatgagaat ggtatagagg gtggcgaaat atgaaaaaaa tgaaaaagta taaaattaga
       61 aaatctggca atagcgatgt aacaactatc ccaccagaag taaaagaatt catgggtgtg
      121 cagacaggtg acgcaatcag ttatgttttt caatctgatg gttctgtacg aatgatcaaa
      181 gcacaagaag aacctgatat tgattcatta gttgattcga tcatgaatca atatgaagac
      241 gcgttaaaag atttggttga tttatgaggt atctttcgac taaagaaatt atcaaaatca
      301 acgcgaaagt tattttacgt cactcgccag gcgaaatgat cggtgtaaaa gatgcaaatg
      361 cattagatat ggccgtcaaa cagccttcac aagcagtatt caatcaagaa ctctatcctg
      421 aaatctatga aaaagcggcg attttagcaa ttaatttagc taaaaaacat ccttttcata
      481 atggcaataa acgaactgct cttgttgcca tgttgttgtt tcttcaattg aataattgtc
      541 cagttgcttt ttctcgtcaa gaagctgttg attttatcat aatgattacc actagttcat
      601 tggatttcga ttcactaaag tccaagataa ccagttattt acgacaaacc gcaataaaat
      661 gaacgaatta aaaactttat ttctcaat
//
LOCUS       DAZKLA010000294          678 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000294 DAZKLA010000000
VERSION     DAZKLA010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.306"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACG85N_002983"
     CDS             132..344
                     /locus_tag="ACG85N_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225651.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>678
                     /locus_tag="ACG85N_002984"
     CDS             506..>678
                     /locus_tag="ACG85N_002984"
                     /inference="COORDINATES: protein motif:HMM:NF015824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0225652.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDS"
BASE COUNT          245 a          101 c          117 g          215 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattc
//
LOCUS       DAZKLA010000295          675 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000295 DAZKLA010000000
VERSION     DAZKLA010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.307"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..>675
                     /locus_tag="ACG85N_002985"
     CDS             28..>675
                     /locus_tag="ACG85N_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0225653.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDP"
BASE COUNT          180 a          116 c          125 g          254 t
ORIGIN      
        1 aaattttcaa aagaaaagag gctagaaatg aaaaaattaa atttatttac agaattgtgc
       61 ggaatcattt taggcgcagg aatctacgga ttagctgtga caggaatcaa tattccatct
      121 aagctggcag atggtggggt tactggtatt gctttgcttt tgcataatct ttttggattc
      181 gctccttctg tcacttcact gctttttaat ttacctttat tgcttatctc attgtttctt
      241 tttggaaaac acgcttttct ccggacgatc gtcggaacat ttgcgctagt tttcttttta
      301 catttatggg agagccttca tatacatttt gctgtagata gtctactgat caatagtttg
      361 atgacaggta ttctttctgg cattggttgc ggtctcgtct ttcgattcgg cggaagcaca
      421 ggcggaacag atatcattta tcaagccatt gaaaaatatt ttcatattaa gattggcaaa
      481 agtcttttct tgattacttt tggtatttta gttgtctcct tgctttattt ggatctcaca
      541 cattttctgt atacattact tagctgttct attctatctt atacattgaa taaagtgaag
      601 tatctggaaa ttggatctgt tttgccccaa ttggaagcaa aacagactac agaaacattt
      661 gaaccacttg atcct
//
LOCUS       DAZKLA010000296          673 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000296 DAZKLA010000000
VERSION     DAZKLA010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.308"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          232 a           82 c          150 g          209 t
ORIGIN      
        1 tttacgggat ataaaaatag tttacgtcga tacttattaa gttatcgacg ttttttatta
       61 tagaaaaaat ttataaaagg aggtttcttg atatgataag aaacaaaaag ggtttgttga
      121 aagttgcttt actggcagct tttttctaag taactttttg acaagctcaa tggcaacaca
      181 agcggaagaa gtaatacaag aactaactgt tatgaataac agctagataa aatgggctat
      241 gtatgttatc caacaattta gtaatcacta gaagcctagc gaaagttcgc ttgataagtt
      301 atagatgagt attgatttgt agtgtttcca aatcaaaagg gtacagagcc acatgaaata
      361 tgttgtcctc tgcagcgtca ggatgggaat ccataccatg gagattcgga gatctgtgag
      421 ggaatactta cgtggttaat atgtcagttg tgttggacgt agtaatggtc atagtgggta
      481 gattgactga attcttatat gagaaaatga aaccaacaag aagatagggt tatttataca
      541 gaattgggga tcttttctgg aaaagtaatc ggataatgcg agattcgtac ggtctttttt
      601 aaatttctct aaaaaaaacg gcatcgaatg ggttgggtga ttgataagca atgaatcata
      661 gactgatata gaa
//
LOCUS       DAZKLA010000297          671 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000297 DAZKLA010000000
VERSION     DAZKLA010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.309"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>671
                     /locus_tag="ACG85N_002986"
     CDS             <1..>671
                     /locus_tag="ACG85N_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0225654.1"
                     /translation="NIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKG
                     AHFFDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGII
                     LSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMA
                     PKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIF
                     IPRLG"
BASE COUNT          211 a           84 c          129 g          247 t
ORIGIN      
        1 tgaatattgc aatctctgta gctttgatgc agacattata ttttgatgat aaatatgttg
       61 gcagagtagt cggcggaaca ctgtcatttg tgttgatagg tttagtaatt tatttaagaa
      121 taatgataaa gggtgctcat ttttttgata aaaaacagtg gagatacgct ttaaaaatct
      181 ctttgccgct aatcccacat tccttagcaa atatagtttt agcacaattc gatagaatta
      241 tgattaattc ttatttaggt cctgctgcgg ggggaatata ttcctacatt agtaacatag
      301 gtattattct aagcgtaata tggatgtcta ctaataacgc ctgggttcca tggttctatg
      361 gtgagatgag taaaaataat gaaaaaaaag tgaagaaagt tagtaactat tatttagtag
      421 tctttacttt gattacgatg attgtaatgg tagtttcgat tgatttagca aaatttatgg
      481 ctccgaaaga ctattattct gggttatctt tagtggttcc gataacctta ggctatttct
      541 ttcagttttt atatagttta ccagttaatg ctgaatttta tgagaaaaaa acatcgttta
      601 ttgcgattgg tacagtagca agtgctatag ttaatatact attaaacatg attttcatac
      661 ctaggttagg a
//
LOCUS       DAZKLA010000298          656 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000298 DAZKLA010000000
VERSION     DAZKLA010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.310"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACG85N_002987"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACG85N_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFQ0225655.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          198 a          143 c          111 g          204 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaa
//
LOCUS       DAZKLA010000299          582 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000299 DAZKLA010000000
VERSION     DAZKLA010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.312"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>582)
                     /locus_tag="ACG85N_002988"
     CDS             complement(37..>582)
                     /locus_tag="ACG85N_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0225656.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          166 a          110 c          100 g          206 t
ORIGIN      
        1 aatccactcc ttttttcatg ctctaaaatg atatttttac acaactgtta cggttgtttt
       61 acgaaattgg acgggacttt gccagtttaa tttctgtttt atccgttcat tattgtagta
      121 atagatatag ctatcgatgg ctgattttaa ttcctcgaaa cagttataaa ctttcccgtg
      181 aaaaatctct tgttttaata agccaaaaaa attctccata agtgaattat ctaagcagtt
      241 ccctttccga gacatgcttt gaaagatttt gtactccttc aatttgtttc tataagcttt
      301 catttgatac gcccaacctt gatctgagtg aaacgtacga cgatagggac aatcgttagt
      361 ggtctggata gcttcctcca aaccttccat taccgctaat gcattgggct tttcagagat
      421 acgataggat aaaatctcac tattgaacat atccataaaa ggatctagat acaatttctt
      481 ttgacggata atgcctttgg catccacttc atggtattta aattcagttg tatccgtcgt
      541 gattttctga tggcagatac tggtgtagaa acgacgatgg at
//
LOCUS       DAZKLA010000300          558 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000300 DAZKLA010000000
VERSION     DAZKLA010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.314"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..362
                     /locus_tag="ACG85N_002989"
     CDS             168..362
                     /locus_tag="ACG85N_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225657.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          236 a           70 c           89 g          163 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtca
//
LOCUS       DAZKLA010000301          538 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000301 DAZKLA010000000
VERSION     DAZKLA010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.315"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>538
                     /locus_tag="ACG85N_002990"
     CDS             <1..>538
                     /locus_tag="ACG85N_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225658.1"
                     /translation="FIAIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLY
                     AHASNVFDSLTEAMNNDLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLA
                     TTSLVPWYLLQNTPAKLLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMT
                     VLLLITLTVSSKKNFNQK"
BASE COUNT          181 a           83 c           82 g          192 t
ORIGIN      
        1 attttattgc tatatttaaa aaattattta gtgcggcagg aatgacattg gttctgacga
       61 gtgctttttg gtatccgatg tttgaacaaa tgctctacca aaaaatcaac aaacctagcg
      121 tgacaaattt atatgctcat gcatctaatg tgttcgatag tctgactgaa gctatgaaca
      181 acgacttaac tacttactca atgggactag ttggtctgtt atctttatgt attcccctga
      241 ttttatttaa aaaattaaca aacatagaaa aaaagattta ttatggtacg tgtttgactt
      301 ggctagctac aactagtttg gtgccatggt atcttctgca aaatacgcca gccaaattgt
      361 tacaatttcc ttggcggatc ttaagcttgc aaataatatt ctcttctctg atattaacga
      421 tgatattttt caaaaataga agatacaata agacaagaga actattttat ttatttatga
      481 ctgttttgtt attaatcact ttaactgttt ctagtaaaaa aaatttcaat caaaaaat
//
LOCUS       DAZKLA010000302          513 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000302 DAZKLA010000000
VERSION     DAZKLA010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.316"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..374
                     /locus_tag="ACG85N_002991"
     CDS             <1..374
                     /locus_tag="ACG85N_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0225659.1"
                     /translation="SSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSF
                     ARKIGQALAGGVGGWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFF
                     LYPLSKDKVLENCELLKQKHK"
     gene            384..>513
                     /locus_tag="ACG85N_002992"
     CDS             384..>513
                     /locus_tag="ACG85N_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0225660.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFL"
BASE COUNT          162 a           64 c          104 g          183 t
ORIGIN      
        1 ttagttcggt tggctatatg ggtttgggtg tgtttaatac tgttatttgg gcaaatatta
       61 ctgatgttat tgatgatcaa gaagtaaagt ctgatcaaag agaagatggg accgtatatg
      121 cggtatattc ttttgctaga aaaataggtc aagctctagc tggaggcgtc ggaggttgga
      181 ctctttctat tataggctat gactctttag caaatgtaca aacgggagat gttttagaaa
      241 agttatataa cgcttcaaca ttaattccat caatttgttt tctgattgta ggattaattt
      301 tatttttcct ttatccatta tctaaagata aagtcttaga aaattgcgag cttttaaaac
      361 aaaaacataa ataggagaga aatatgaata attggatatt ttgtattgat tcagatggct
      421 gtgttatgga tacgatgact tataaacaca atctcttttt cggaccaatt gctgcagaga
      481 tttttgatgt tcatcataga gaaaaattcc tac
//
LOCUS       DAZKLA010000303          512 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000303 DAZKLA010000000
VERSION     DAZKLA010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.317"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..343
                     /locus_tag="ACG85N_002993"
     CDS             <1..343
                     /locus_tag="ACG85N_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0225661.1"
                     /translation="DNIRDKKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDL
                     IRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRA
                     SLYRKLSEVNN"
BASE COUNT          206 a           69 c           93 g          144 t
ORIGIN      
        1 cgataacata cgagataaaa aggcaagttt aaaatcacta aaagatacat ggcttgattt
       61 atcagaagat aatccataca gccaattctt aattactgta atggctggtg ttaaccaatt
      121 agagcgagat cttattcgga tgagacaacg tgaagggatt gaattggcta agaaagaagg
      181 aaagtttaaa ggtcgattaa agaagtatca taaaaatcac gcaggaatga attatgcggt
      241 aaagctatat aaagaaggaa atatgactgt aaatcaaatt tgtgaaatta ctaatgtatc
      301 tagggcttca ttatacagga aattatcaga agtgaataat tagccattct gtattccgct
      361 aatgggcaat atttttaaag aagaaaagga aactataaaa tattaacagc ctcctagcga
      421 tgccgaaaag ccctttgata aaaaaagaat catcatctta agaaattctt agtcatttat
      481 tatgtaaatg cttataaatt cggccctata at
//
LOCUS       DAZKLA010000304          500 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000304 DAZKLA010000000
VERSION     DAZKLA010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.318"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..254
                     /locus_tag="ACG85N_002994"
     CDS             <1..254
                     /locus_tag="ACG85N_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFQ0225662.1"
                     /translation="GEIKVVGIRPGEKLYEELMTTDEIERSYENDKMYIIPPHYHATD
                     ELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVTW"
     gene            266..>500
                     /locus_tag="ACG85N_002995"
     CDS             266..>500
                     /locus_tag="ACG85N_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFQ0225663.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELS"
BASE COUNT          207 a           64 c           96 g          133 t
ORIGIN      
        1 gaggtgaaat aaaagttgta ggtatcaggc caggagaaaa attatatgaa gagctaatga
       61 ctacagacga aattgaacgc tcatatgaaa atgataaaat gtatatcatt ccaccacact
      121 atcatgctac tgacgaactg tacaaagatt atgaaaaaat taaaatcact gcatactctt
      181 cagatgcaac agaattaatg agtggacagg aatttattag aatcgcaaaa gagaataatg
      241 tcgtgacttg gtaaggagtt ttatgatgaa atcagaagta tatgtaattg cagaaattgg
      301 tgtaaaccat aatggaagtc tagatttagc tttagaaagt attgataaag ctaaagaatg
      361 tggagctaac gcggttaaat ttcaaacatt taaaacgaat aagctagttt ccaaaaaggc
      421 tccaatggca aaatatcaga aggataatct taaagaggac agttctcaat ttgaaatgct
      481 aaaaaaatta gaattatctc
//
LOCUS       DAZKLA010000305          497 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000305 DAZKLA010000000
VERSION     DAZKLA010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.319"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          165 a           96 c           58 g          178 t
ORIGIN      
        1 taagaatgct aaatcactct acaataacgg cgaaagcaca aaaaataaaa aaattattac
       61 gttattaaaa aaacagatga aagatcaggt tttattagat ctcattcttt catcacttga
      121 agggatttaa tgaactactt tttcctcttt gtcgttttta tatattcgta ttccttcaaa
      181 cagtaggata gcctctttca atgcttgata tttgaactgt ccgttactta aaaatttaga
      241 tgtgccgccc tcttcctaga acatattctt ttttactttt tcattaattg acagaccaat
      301 cgaacgaaac tgtaaatttc gataggataa tgattggtca aactcattca ttttcaagac
      361 atctcgattc atattcaaga taggaatctt tttatcaaca tcactttatt cgtattttcc
      421 tctaccataa aagcttcacg ctccacaaaa ttgttttata cactactatt cagcccattc
      481 aacgaccaat gaatttt
//
LOCUS       DAZKLA010000306          495 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000306 DAZKLA010000000
VERSION     DAZKLA010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.320"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..444
                     /locus_tag="ACG85N_002996"
     CDS             <1..444
                     /locus_tag="ACG85N_002996"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFQ0225664.1"
                     /translation="LEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQLEELYQAYQT
                     QGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTDPLFTYLKNE
                     APGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          177 a           95 c           75 g          148 t
ORIGIN      
        1 ttagaagacg gcactgtcta ttcattgaaa aaatatgcag gcaaaccgat aatcattgtt
       61 aacactgcaa caaaatgcgg tctagcacct caatttaaac aattagaaga actttaccaa
      121 gcttatcaga cacaaggtct ggttatctta ggctttcctt ccaatcaatt taaacaagag
      181 ctagattcag cgactgaagc agcagaagct tgtaaaacca cttatggcgt taccttccct
      241 atgcatcaat tgattgacgt aaatggagaa aatacagatc ctctttttac ttatcttaaa
      301 aatgaagctc ctggtactgt cactaaagca ataaaatgga attttactaa atttttgatt
      361 gatagaaatg gcaaagtaat cgaacgattt gcaccacaaa cttctccatt gaaaatgacc
      421 gctgcaattg aatccgttct ttaacttaat aaaaataaaa aaagatgaac tatttctagt
      481 tcatcttttt gcctt
//
LOCUS       DAZKLA010000307          485 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000307 DAZKLA010000000
VERSION     DAZKLA010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.321"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..425)
                     /gene="spxA"
                     /locus_tag="ACG85N_002997"
     CDS             complement(27..425)
                     /gene="spxA"
                     /locus_tag="ACG85N_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFQ0225665.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          140 a           98 c           84 g          163 t
ORIGIN      
        1 tcttttccaa gtttcgtatt atccttctat aatccagcca tcaattgtgc ttgacgtaat
       61 tctaattgtc gtacatcgcg cggtaggaac cgacgaattt catcttcatt aaaaccaact
      121 tgcagacgtt tatcatcaat catgattgga cggcgaagca atccaggatt gttttgcaca
      181 agttccaata attcttgtaa cggcagatca tctaaatcca tgttcagttt ttggaatact
      241 ttggaacgtg tcgagatgat ttcttctgtt ccgtcttctg tcatttgtaa aatcgctttt
      301 aattcagaac tgttcaatgg ttctgaaaaa atattacgtt caacaaatgg aatctgatgt
      361 tcttgcaacc acgcacgagc ctttctgcag gaagtacaac ttggggaagt atataatgtt
      421 aacaacgtgc accactcctt ttcgtgataa gaatcacttt tttgtaacag aagatcataa
      481 taatc
//
LOCUS       DAZKLA010000308          483 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000308 DAZKLA010000000
VERSION     DAZKLA010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.322"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..428
                     /locus_tag="ACG85N_002998"
     CDS             129..428
                     /locus_tag="ACG85N_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0225666.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          185 a           59 c           97 g          142 t
ORIGIN      
        1 aatcgttgcc ataacagtgt attgaaaggt tttaacaatc cagtaatgat ttaaaaaaat
       61 ctagatgtag cgtatacttt gtttatagaa tgtagtggga agaaatatat ttttgggagg
      121 aaatcgtgat gtctaaaagt gaagaaatag aaaacatggt actaaaatta tatgatctcg
      181 ttttaaatcc agagatcaaa gaaaaggaac gtatactttt gatagacgca aagacaggct
      241 tagaaaaagg tcagtattat cctaaagtca taaataattt ggaacgtagt ttacgtccgt
      301 tagcaatcag gggagaattg tctaaaccag tatcaccgtt ttatatggag atatcaacaa
      361 taggaaaatt tgaaaaagaa ttaggtcgag gaatggcttc ggcaccgatt acttttgggc
      421 atttataaaa acgagttatt catctcaatg aattaaaaag tatagtcaat ctcataatgc
      481 agg
//
LOCUS       DAZKLA010000309          483 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000309 DAZKLA010000000
VERSION     DAZKLA010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.323"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..99)
                     /locus_tag="ACG85N_002999"
     tRNA            complement(26..99)
                     /locus_tag="ACG85N_002999"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(63..65),aa:Ile,seq:cat)
     gene            complement(266..>483)
                     /locus_tag="ACG85N_003000"
     CDS             complement(266..>483)
                     /locus_tag="ACG85N_003000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225667.1"
                     /translation="LNHNCVFPSISIKVHLKKSVRTKVLFVNLKIDFPEYVLGISKCL
                     NVFLNAKSMQKILYFSTFLSLTKIKKR"
BASE COUNT          164 a           77 c           96 g          146 t
ORIGIN      
        1 agaacgattc aattggctac attaatgtac cctgtaggac tcgaacctac gaccggacgg
       61 ttatgagccg tctgctctga ccaactgagc taagggtagt ggttgttgcc acataaagcc
      121 ataaacaatc aaccagtaga atgcgtggca acaaacctgt tatcgcatat cttggagtgt
      181 gactatttat gagtgatagt gaagatatgc gtctccatat atgtatggtt gagacagata
      241 aacatgtatg aaaaacgttg ttaaatcaac gtttttttat tttggtcaag gagagaaaag
      301 tagagaaata caaaattttt tgcattgatt ttgcattcaa aaaaacattc aaacatttag
      361 aaatccccaa tacgtattca ggaaaatcta ttttaaggtt tacaaaaaga acttttgttc
      421 gtacactctt tttgaggtga acttttatgc tgatggaagg aaaaacacaa ttatggttta
      481 agc
//
LOCUS       DAZKLA010000310          482 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000310 DAZKLA010000000
VERSION     DAZKLA010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.324"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..398
                     /locus_tag="ACG85N_003001"
     CDS             141..398
                     /locus_tag="ACG85N_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225668.1"
                     /translation="MSKLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          209 a           64 c           68 g          141 t
ORIGIN      
        1 ttctgtccac gttcatatta ctccaaagtt ttcctcattc caaaagaaaa gcacagaata
       61 cacaaagaag cgtttttatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaaat tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 acaaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tt
//
LOCUS       DAZKLA010000311          476 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000311 DAZKLA010000000
VERSION     DAZKLA010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.325"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..109
                     /locus_tag="ACG85N_003002"
     CDS             <1..109
                     /locus_tag="ACG85N_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0225669.1"
                     /translation="FDKTDKEIGKVLGITRQGATNLKHRLYKKIQKMLV"
     gene            203..394
                     /locus_tag="ACG85N_003003"
     CDS             203..394
                     /locus_tag="ACG85N_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0225670.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          181 a           65 c           82 g          148 t
ORIGIN      
        1 ctttgataaa actgataaag aaattggaaa ggttctgggg attacaagac agggtgcgac
       61 aaatttaaaa catcgacttt acaaaaaaat tcaaaaaatg ctggtttaat atctgcataa
      121 taactaattt tgtatgcttc tttgctcgtt ctcctccttc ccaaagaaat agattataag
      181 tccatgggaa gaaggtagat ttatgaattc tttaaaagaa gatttcattt tagcaaaggc
      241 tggaaatgaa gaagctgttg aggcaattct caaaagattt agctctttga ttcataagca
      301 atcctggaga actggaaaat atgatcaaga ttgttatcaa gaatgtatgc ttgcaatata
      361 tctagctatc tcgaaattcg aaattaaaga ataactcaac taagggagtc tgtgacataa
      421 gtaaaaagat agtttcaaat ttcgaacaat aaatttgaaa ctatcttctt tataat
//
LOCUS       DAZKLA010000312          468 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000312 DAZKLA010000000
VERSION     DAZKLA010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.326"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>468
                     /locus_tag="ACG85N_003004"
     CDS             <1..>468
                     /locus_tag="ACG85N_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFQ0225671.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAZKLA010000313          455 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000313 DAZKLA010000000
VERSION     DAZKLA010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.327"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>455)
                     /locus_tag="ACG85N_003005"
     CDS             complement(<1..>455)
                     /locus_tag="ACG85N_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0225672.1"
                     /translation="YPRINRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHGSQ
                     WWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDL
                     PLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVRKV"
BASE COUNT          146 a           66 c           62 g          181 t
ORIGIN      
        1 acttttctta caaataattt ttcactttct tttatttgat ctatatccct ttcatcaaac
       61 catttgttca tggaaggatc aataatatga atatttgcta atttcgaagt ttgtaaagta
      121 gtttcttcaa tatttttcaa ttcaagtggt aaatctggtc ctccaataac atttttattt
      181 ttgaacgagg aattaaaaaa aagtgtatga aaaaattttt catctggggc aaacatacct
      241 attttaaaat tcaggtagtc ttgttgattt tgttcataca tttgtattaa ttctgttagg
      301 cattctttcg acaaagccca ccattgagaa ccatgataca aatcaaattt atggtgtcta
      361 atttttgggg gaagtttaat catcctcaga caacgattaa ttatagctct aaaaatttta
      421 aatgcagttg tgttattcct attaattcta ggata
//
LOCUS       DAZKLA010000314          439 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000314 DAZKLA010000000
VERSION     DAZKLA010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.328"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..270)
                     /locus_tag="ACG85N_003006"
                     /pseudo
     CDS             complement(34..270)
                     /locus_tag="ACG85N_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          144 a           74 c           74 g          147 t
ORIGIN      
        1 agaaggactg accttctgtc tcagattttt ttgttatttc acttgaatca attgattgat
       61 caaaaaaatc ctgattttca tgttctcaaa tgatttaaat ccataagata atcgtttcag
      121 tgtcttgata tggggaatct tggcctctat tttgccatta gaatacgggt aaatcatagc
      181 attgtaaata ccttcttcat acgttaaaag gttttgaagt ttcgtgcgaa gttgtgcatc
      241 aagtgtttca ggaagatttt ttaataacgc cgtttgtata attaagcgag acaaataaaa
      301 aagccattta tgttacactc caattgtaat acccgctaaa gcatacaagg agagtgaaca
      361 taaatgactt atacccatct tacaccaaac gagcttgtaa tgatagaagc atattttcat
      421 caagaaactc cggttgcta
//
LOCUS       DAZKLA010000315          431 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000315 DAZKLA010000000
VERSION     DAZKLA010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.329"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            9..323
                     /locus_tag="ACG85N_003007"
     CDS             9..323
                     /locus_tag="ACG85N_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225673.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          140 a           54 c           66 g          171 t
ORIGIN      
        1 gatagctttt ggatttttac agtaatttta tcttaataat agcaatatta ttacttctaa
       61 atatttggtt ttttgataag agtagaaatg cagggatagg ttttagaacc aaacgaagta
      121 cgtcttctga aaaaaagtgg gtatattcac agactatttt ttacggaggg gttatttcaa
      181 tcagtttact ttcttcaaca ctttattctt ttaatgttat agacgtttcc atgtcaaatt
      241 ttatatctat tattggaatc cttatttctg caattattac acaactactt cttgtgtttg
      301 aagagaagtc aaaaaataat taaagcaatt atgcctttta gtacaggaat tattacttct
      361 tacaaatgta tagcgaatgt gaagcctgct gttttaattg ttctagaaaa acttttgagt
      421 gaacaattat t
//
LOCUS       DAZKLA010000316          425 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000316 DAZKLA010000000
VERSION     DAZKLA010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.331"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          123 a           75 c           55 g          172 t
ORIGIN      
        1 ttatttcttc acatttatta ggttatagtt taagaaagtt taaacattgc gcaatttaat
       61 tattttagct gtttcttttc ataaaaaaac atgttgtgac tctgagacaa atcatagtcc
      121 atagtgatgt ctctagagta actacgccta atattacgag gcatcatatt aatctatcag
      181 cactagtacc taaactagca tccctttcga atattttttc ttatataata aagtttgctt
      241 tcactcctct aatctcacca aatcgactgg cttgcgttgt gtgatactag gaactgattg
      301 gtttagaacg acttttctat tgtaatcata tttttcgcca tcctcgttgt cttctaatcc
      361 ttcaatgaat gatccctaat gtgtgtatta aataatttaa tttttttgtt cctatttatt
      421 taaca
//
LOCUS       DAZKLA010000317          421 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000317 DAZKLA010000000
VERSION     DAZKLA010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.333"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..355)
                     /locus_tag="ACG85N_003008"
                     /pseudo
     CDS             complement(170..355)
                     /locus_tag="ACG85N_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
BASE COUNT          120 a           85 c           62 g          154 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagaggtt
      361 ctgttgcgaa gtttgaaaat caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt
      421 a
//
LOCUS       DAZKLA010000318          413 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000318 DAZKLA010000000
VERSION     DAZKLA010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.334"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..>413
                     /locus_tag="ACG85N_003009"
     CDS             153..>413
                     /locus_tag="ACG85N_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0225674.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIF"
BASE COUNT          148 a           72 c           59 g          134 t
ORIGIN      
        1 actaaaaaaa catttttgaa aataaacatc agcagtccta tcaatgcatg tgagcttata
       61 gaagttattc gcagcgcagc ataattgcat tagcttcttt tcgtgttttt tgcctctttt
      121 ccctttaaat cttaatggag gaggtgtgca atatgtcaga taacgacgaa cgcgtaaatg
      181 cccagttgat tcgattcttt gaaaaggtca ttttgaacac cgctaatact tattataaaa
      241 aacaagaaga aattcaaaaa catgaacaac ttgatcaact cccaccatat ttgtttacta
      301 aagatactat aaaatttaaa caaccgatta atgttttaaa tgtgactctc cttgttgaag
      361 atataaaatt agcaaaaatc cttcctttgt tgaaagaacc tgaacaaatt ttt
//
LOCUS       DAZKLA010000319          402 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000319 DAZKLA010000000
VERSION     DAZKLA010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.335"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..150)
                     /locus_tag="ACG85N_003010"
     CDS             complement(<1..150)
                     /locus_tag="ACG85N_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0225675.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYES"
     gene            complement(169..330)
                     /locus_tag="ACG85N_003011"
     CDS             complement(169..330)
                     /locus_tag="ACG85N_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFQ0225676.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT          134 a           76 c           42 g          150 t
ORIGIN      
        1 actttcatat aaagcctttt ctatttcatt gcttgatttc attattgtta aacaagaata
       61 caagcaagaa agaactatag ttaatatgat aaaaattatt aaagttctat ttttctttct
      121 tgtaatatat gctatagcat tttttatcac tttcctaact actcctctct attttcttac
      181 tccataacta attgcatcca ctggacaagc atttttacat cgtccacatc tgatacactc
      241 cagatgattg caattctcaa ctgaatcttt attcatttgg caaacttttt tacattttcc
      301 acaattaata cacttatcct tatcctgaat cggctacact agactagaca gaaaaaataa
      361 ggtgtgtaga ataataaaaa acacactgga ggatttttca tg
//
LOCUS       DAZKLA010000320          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000320 DAZKLA010000000
VERSION     DAZKLA010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.336"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACG85N_003012"
     CDS             <1..>386
                     /locus_tag="ACG85N_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0225677.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLIKQSKRLFPEINCVVADA
                     EYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTRERYREYKSNPKECVNCPLLNQCT"
BASE COUNT          120 a           80 c           67 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctca tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgagtata agacacctaa gtttgttcac tttttgactc atttaaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gggctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaaa ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAZKLA010000321          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000321 DAZKLA010000000
VERSION     DAZKLA010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.337"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACG85N_003013"
     CDS             <1..>386
                     /locus_tag="ACG85N_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0225678.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCT"
BASE COUNT          117 a           80 c           70 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctcg tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgggtata agacacctaa gtttgttcac tttttgactc atttgaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gagctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaag ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAZKLA010000322          385 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000322 DAZKLA010000000
VERSION     DAZKLA010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.338"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            98..>385
                     /locus_tag="ACG85N_003014"
     CDS             98..>385
                     /locus_tag="ACG85N_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225679.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERR"
BASE COUNT          146 a           52 c           76 g          111 t
ORIGIN      
        1 ttgtgcatta ctgaagaaga agcagataaa atcattagca aagaagagag cgcatcttaa
       61 ttgatgtgct ttttattttg attcaaggag ttgtcacatg attaatttag gggaatgggg
      121 agcgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa taaaaccaat
      181 tactgcatct ttctcgaaga ttactgagac tctttcaaaa gtaagtcaca atttagattt
      241 gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg aagaactaaa
      301 gaaacacgat gaaagattag atacacatac agaaaaattg gtggaacaca cgcaacaaat
      361 taaaacttta tttagggaga gaaga
//
LOCUS       DAZKLA010000323          379 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000323 DAZKLA010000000
VERSION     DAZKLA010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.339"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            203..379
                     /locus_tag="ACG85N_003015"
                     /pseudo
     CDS             203..379
                     /locus_tag="ACG85N_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT          144 a           47 c           66 g          122 t
ORIGIN      
        1 aaaccaaatt taaactttat attataataa tgaagcttaa tcttctttat agagaattgg
       61 gctattttta atggcaaaga ggggaagaag atgtttcaaa acaaaggaca acgctatgtt
      121 gtaaaaggag taaaggaatc ttactggaat caaaaatctt tattgagaaa attatctttg
      181 aatttaagca caaagaaatg aaggtacact agatagcaat gaagatatct ttgtcattaa
      241 acgtcgctat tttacacaag taagtgatga tttgattcat gtcttttcca atgagtggtt
      301 tgtttctgag aaaaaattac atgcttctaa tctgcaatat atgccaggtg aagatccaat
      361 tcctaatatg aaagcaatt
//
LOCUS       DAZKLA010000324          359 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000324 DAZKLA010000000
VERSION     DAZKLA010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.340"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..277
                     /locus_tag="ACG85N_003016"
     CDS             <1..277
                     /locus_tag="ACG85N_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFQ0225680.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
BASE COUNT          123 a           63 c           77 g           96 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataa
//
LOCUS       DAZKLA010000325          357 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000325 DAZKLA010000000
VERSION     DAZKLA010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.341"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..181
                     /locus_tag="ACG85N_003017"
     CDS             59..181
                     /locus_tag="ACG85N_003017"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225681.1"
                     /translation="MHKPFPDPIQKAIDELGIKNKKRFILVIHYMTCKVRKLVG"
     gene            154..285
                     /locus_tag="ACG85N_003018"
     CDS             154..285
                     /locus_tag="ACG85N_003018"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225682.1"
                     /translation="MQSAKACGIAFGLAKWGAKNIENFQSVDIIIDSPQEILDLLEK"
BASE COUNT          127 a           51 c           68 g          111 t
ORIGIN      
        1 ctagcaccac gggttaaatc tatattttga tatacaaatt acagcttcag atactaagtt
       61 gcataaacct tttccggacc ccatacaaaa ggcaattgat gaattgggca taaaaaataa
      121 gaaacgattt atattggtga ttcattatat gacatgcaaa gtgcgaaagc ttgtgggata
      181 gcatttggtt tagctaaatg gggagccaaa aatatagaaa attttcaaag tgtagatata
      241 attattgatt ctcctcaaga aatacttgat ttgttagaaa aatagacaaa gagcgtgaga
      301 taaggatagg gtctcacgct ctttgttttt cgccatttaa agattgaaat ttttgcc
//
LOCUS       DAZKLA010000326          348 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000326 DAZKLA010000000
VERSION     DAZKLA010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.342"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..329)
                     /locus_tag="ACG85N_003019"
     CDS             complement(<1..329)
                     /locus_tag="ACG85N_003019"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225683.1"
                     /translation="MDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSII
                     YRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYT
                     VTTIISYL"
BASE COUNT          101 a           67 c           51 g          129 t
ORIGIN      
        1 agataagaaa taatagtcgt tactgtataa cttgtgctta ctttaatatc agcaactcct
       61 ttgactccgc ttatttgtac caatccatta gcattcacct gctcctttac aggttgatga
      121 tttatttcta cattcgttcc taaataagca tatacaggaa gtgtgattac ttggctcttc
      181 tcattagtta ctctatatat gatagaacta ccagttgttt tataaggaag gtttacccat
      241 tgactgccaa tctttgcttc gtgttttatt atatgctcct tattctccaa tgaagcgata
      301 ggagaataat catataaaaa ataatccatt cctttagtgg tatacgcc
//
LOCUS       DAZKLA010000327          341 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000327 DAZKLA010000000
VERSION     DAZKLA010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.343"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>341
                     /locus_tag="ACG85N_003020"
     CDS             <1..>341
                     /locus_tag="ACG85N_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0225684.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEG"
BASE COUNT          141 a           50 c           76 g           74 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DAZKLA010000328          337 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000328 DAZKLA010000000
VERSION     DAZKLA010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.denovo.344"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          135 a           34 c           58 g          110 t
ORIGIN      
        1 aatagggaat tttctattgc aattttataa agagattgct atattattat ttgttctaaa
       61 tgattccttt aaggtgtcac taaaaattat aaaaaatagt gttgacgcac ttttgatgtc
      121 atgttataat aacgaagtcg tttggatgag atagctaata aagcttgatt ttatcgaaaa
      181 aaacttcgaa aaagttattg acaaataaca ataaagatgg tatgataatc aagtcgctgc
      241 taatgagtgg caaagacgtt tagaaaaaat aaacaaagaa gttgttgaca ttctcttctg
      301 aatctgataa gattaaagag ttgttaaaac aaaaaga
//
LOCUS       DAZKLA010000329        17069 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.guided.053, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000329 DAZKLA010000000
VERSION     DAZKLA010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.guided.053"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..577)
                     /locus_tag="ACG85N_003021"
     CDS             complement(134..577)
                     /locus_tag="ACG85N_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFQ0225685.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ACG85N_003022"
     CDS             complement(795..2135)
                     /locus_tag="ACG85N_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0225686.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ACG85N_003023"
     CDS             2404..3300
                     /locus_tag="ACG85N_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0225687.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ACG85N_003024"
     CDS             complement(3450..4391)
                     /locus_tag="ACG85N_003024"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0225688.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="ACG85N_003025"
     CDS             4517..5638
                     /locus_tag="ACG85N_003025"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0225689.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG85N_003026"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG85N_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0225690.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="ACG85N_003027"
     CDS             7465..7992
                     /locus_tag="ACG85N_003027"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0225691.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="ACG85N_003028"
     CDS             8203..9264
                     /locus_tag="ACG85N_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0225692.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="ACG85N_003029"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="ACG85N_003029"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0225693.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="ACG85N_003030"
     CDS             10177..12159
                     /locus_tag="ACG85N_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0225694.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="ACG85N_003031"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="ACG85N_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0225695.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="ACG85N_003032"
     CDS             12881..14305
                     /locus_tag="ACG85N_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFQ0225696.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="ACG85N_003033"
     CDS             complement(14486..15259)
                     /locus_tag="ACG85N_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0225697.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="ACG85N_003034"
     CDS             complement(15256..16161)
                     /locus_tag="ACG85N_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0225698.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="ACG85N_003035"
     CDS             complement(16315..16635)
                     /locus_tag="ACG85N_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFQ0225699.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         5520 a         3104 c         3493 g         4952 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    16921 tatttctata tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat
    16981 aatttttgtg ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt tcgtgagaaa
    17041 acaactatat tttttatgtg ttttgcttt
//
LOCUS       DAZKLA010000330         8652 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.guided.115, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000330 DAZKLA010000000
VERSION     DAZKLA010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.guided.115"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..1009)
                     /locus_tag="ACG85N_003036"
     CDS             complement(137..1009)
                     /locus_tag="ACG85N_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0225700.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            1137..1706
                     /locus_tag="ACG85N_003037"
     CDS             1137..1706
                     /locus_tag="ACG85N_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0225701.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1773..2510
                     /locus_tag="ACG85N_003038"
     CDS             1773..2510
                     /locus_tag="ACG85N_003038"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0225702.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            2741..4009
                     /locus_tag="ACG85N_003039"
     CDS             2741..4009
                     /locus_tag="ACG85N_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0225703.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            4496..4960
                     /locus_tag="ACG85N_003040"
     CDS             4496..4960
                     /locus_tag="ACG85N_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0225704.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            4944..5573
                     /locus_tag="ACG85N_003041"
     CDS             4944..5573
                     /locus_tag="ACG85N_003041"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0225705.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            5927..7405
                     /gene="msr(C)"
                     /locus_tag="ACG85N_003042"
     CDS             5927..7405
                     /gene="msr(C)"
                     /locus_tag="ACG85N_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0225706.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            7452..7898
                     /locus_tag="ACG85N_003043"
     CDS             7452..7898
                     /locus_tag="ACG85N_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0225707.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(8097..8612)
                     /locus_tag="ACG85N_003044"
     CDS             complement(8097..8612)
                     /locus_tag="ACG85N_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0225708.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
BASE COUNT         2839 a         1533 c         1756 g         2524 t
ORIGIN      
        1 taattatagt aatttactgg aattatcaga aataaaagga aggaacaaat taagaaactt
       61 atgttattta atcattggat cttttgctta acttattcgt tgttaaaaag ctgaaagcga
      121 aggaaaaaaa ctctacttaa ctgtatttct ccgttaggac atctaaaaat ttacgagcag
      181 caggagaaag aggctggtct tttttccaaa tgagattcag tgaagcttgt aatggcggat
      241 cgagtgggat gaatactttt tccgtcccat aagtattgat gatcccatct agagctaatg
      301 caccaaaagt cccgttatcg accatcaaag aagcattgta caataagtta tatgttccga
      361 cgatcgataa ctgatctatg ctgaaaccta gccattcagc tagttgactg tccactgacg
      421 tttgccggga aatgatcaaa ggaacagttt tcagttcctg cggatggatg actgtttgct
      481 ttgctaaagg atgattccga tgtatcaaaa gtccccaacg atcttgggct ggcagtcgaa
      541 atgactggta tttttgtttg tttgtcggtt cgattacgag tccaaaatca agtaatccgc
      601 tatccagttt gtctatgata tcatctgcat tcccgctata taaatgaaac tggatatcag
      661 gatgcgcctt gatcaacgaa tgaaggctat cagaaatgaa agagattgct ttagtttcgc
      721 ctccgccaat gtagatttct ccagagatga ttgcgtcatt tgttaaattg ttcgccgttt
      781 tttctaccaa cgacaagatt tcttttcctt ttgaataaag atatcgacca gaatccgtca
      841 actgtatctc tcgggagcct cgagtgaaaa gtgtcacacc aagttcttct tctaaatctt
      901 tcagttgttt tgagagagta ggttgcgtga tatgcagaga ttttgctgct cggctgatat
      961 ttttttctct agctactgct aaaaaatacg tcagtatacg aatatccatt ttttccctcc
     1021 tgtttttaaa aaagcatacc actaagtata gttaaaagga atagttaagt atgttttata
     1081 agtattagac atcataagcc gtccgttata aagtaaggat aaaaggaggg agcaaaatga
     1141 caagcctcat cgaaaaacaa atcgtcaata aagagattcg agtaacggat ccgctctttg
     1201 aagtgatcca tcagatccaa gcggaaaacg aagagccttt agcagcgctg aatactgggt
     1261 accatgaacc agcagatatc cgcaaacggc tagaaaatat cattagtgac aaagtagatg
     1321 atacggtgac tgttctgctg cctttttata cagatttcgg caaacatatt agtataggga
     1381 aaaatgtctt tatcaatcgt caagtgatgt tcgttgactt aggtggcatc tgtttagaag
     1441 attcagtatt gattggtcct cgtgtcaacc tcatcacggt gaaccatcta accgatccaa
     1501 aagaacgaag aggcttgtca gtaaagccaa tccacatcaa gaaaaatgct tggatcggag
     1561 caggagcaac tattctgcca ggcgtaacga ttggcgagaa tgccattgtt gcagcgaatg
     1621 caactgttac gaaagatgtc ccagacaata ccatcgttgc aggaatccct gccaaaatcg
     1681 tcaaacctgt cgaacggatt atttgaacag taagatgaaa taagagatag tgagtataag
     1741 caagagattt ttcaaaacag gaggagaata gaatgaatga tttatcagaa aaagtgattg
     1801 tcatcatggg cgcttcaagc ggaatcggtg aagcaacaac gaagctatta gcagaaaaag
     1861 gggctaaact agttatagct gcacgccgag aagatcgcct aaaagcaata aaagaatctt
     1921 taccagaagc tgagctatat attcaaacag cagatgtaag agattttgca caagtccaag
     1981 cagttatcga tttagcgatg gaaaagttcg gtcgaatcga tgtattgtac aataatgcag
     2041 gaatcatgcc aaccgctcca ttagttgaag gtcaccgtga tgaatggcaa aatatgcttg
     2101 atatcaacat catgggtgtg ttaaatggaa tttccgccgt acttccaatc atggaaaaac
     2161 aaaaatctgg gcatatcatt tcaactgatt ctgtggcggg acatgttgtt tatcctgatt
     2221 ccgccgttta ttgcggaaca aaatttgctg tccgtgcaat catggaagga ttacgtcaag
     2281 agcaacggca aaataacatc aaatctacga tcatctctcc aggagctgtt caaactgaat
     2341 tatatcaaac catcagcgac aaaaaggctg ctcttgaact tcatgaagcg caaaaagaat
     2401 ggggattaac atctgaagat atagcttctg ctgtagcatt tgcgatagaa acgccagacc
     2461 ggatgtctgt cagcaacatg attatccgtc cgacgacaca ggaagtataa tccataacgg
     2521 ataatatcca gagagaatta cttgttaaat agttattcaa acgaccatca atttaaggcg
     2581 tgaatggaga agaatattct tcatttacgt ctttttatgc agaaaaagca aatttattaa
     2641 aaagaaacaa atgttattta aatatggaca ttgacttgct tttacttttt tagttacact
     2701 gttatttagg aaaagtttca taattcaaag gagaaaaaag atgaaagcaa agaatttatt
     2761 atttagcgtt attcttttag gaaatctagc agttgccaac acaacagtgc tagctgaaga
     2821 atcgacagaa actgtcgcaa ctcagacatc gagttcttca gaaacaggaa ttgaaaacga
     2881 tacagaaagt actttgcagt cagctactgt ctcagaaaat acaatttcaa ccgaagaacc
     2941 aacaactgaa acaacagaac aaacacaaac cgaagacagt acatcagcag aagctcagaa
     3001 caatccagag aatgcagaag aaatcacgaa ccaattgaca gatgggcaaa cattgacaca
     3061 tacccatcaa gcagatttgt ctgaattcga aaactatact ggctatgctt tttatgctcg
     3121 ctcatctgct aattctcaac aagcgttcat tgatagtatt gcctcaactg cacaaagttt
     3181 ggcaagtgcc aatgacttat atgcatcagt catgattgct caagccatcg ttgaaagtgg
     3241 ctgggggaac agtactttgg cttctgcccc taattacaat ctatttggga ttaaaggaag
     3301 ctataatggc caatctgtta cgatgcctac ttcggaatat gtgaatgggc aatggattac
     3361 ggtgaatgcc gcttttagaa aatatccttc gtacaaagaa tcattgcaag ataacgtaac
     3421 ggtattgaag acgacttctt ttcagccagg cgtttactac tattccggtg catggaaaag
     3481 caatacgaac tcttacaaag atgctacggc ttggctaaca ggtcgttatg ccactgcgcc
     3541 taattatggt tctacattga acaatgtaat cgaaacctat aatctgacac agtatgatac
     3601 agcaccaact tcaacttctc cgatgtatcg tctgtataac cgtcatacag gagaacattt
     3661 atatacattg aatgccggag aaaaaaatta tctgccaact gtaggctggg aatatgaagg
     3721 gattgcttgg caagcaccaa acagcggaca acctgtctac cgtttataca atccttacag
     3781 tggagaccat cattacacaa tggcacaatc tgagatcaac tctttaacaa aaatcggttg
     3841 gcgttatgaa ggtctaagtt tttattctgg tggttccaag ccaatttatc gactatttaa
     3901 tccaaatgaa aaaactggta cccatcacta taccttgagt gctaaggaaa gagattggct
     3961 aacaccgatg ggctggagat atgaaggaat aggattttat ggttattaaa aagaggtagt
     4021 gaaaaagctg ccctgcatca agtagtaaga acagccaaac aaaaacagat tttcatgttt
     4081 tttgtttggt tgaacttatt accgcagatg caagtttttg aacaccgttt actcaaaaaa
     4141 ggttgtgaca acatcttgtc ataacttttt tattactgtt taagcatact gatccaattt
     4201 ctaatattcg acgggattct tgcaaaaggc gatttcggaa atttttggcg gtgtaaaatt
     4261 atctactgca gaagagctat cttctatagt ttgataagaa tcaataatct tggttttagc
     4321 gttgtgcttt ctttatcatt ttttccgttc attctacaaa aaatttcttt tctctatact
     4381 tttctattga gagcgttttt tttattacac agctgattct ttgacttttt tcttttttta
     4441 ttctattatt gacaagtcaa tcgttgacga gtcaattatt agaaaggagg aaattatgtc
     4501 tttagaagag attagtcacc ttttatatca aatcaaatta gccgatcaaa aaatcgtcca
     4561 gatctttgaa gaaaatatcg gtataagtct tactagatac gagatcatga tgagactgaa
     4621 agaagaaaat ccattgctac aaagcagttt gcaagaaaag ttgcagattg atcaaggagc
     4681 agttactcgt catctgaagg tattggaagc aaatggctat gtttcgcgtc agagaaatcc
     4741 tcagaacaac cgggaagtgt atgtagcgct aacagaaaaa gcccagaaga aaatcaagca
     4801 ttgtgaaatg gaccataaag atacaggttc cgtgctatcc ccggctttca gcgatgtaga
     4861 aatgaaacaa tttctaacac ttttagataa attgaatacc caactttgca atataacaac
     4921 agaaacgaaa gaggttaata ataatggaac aaaaaaatga tttttcaaca attatgactg
     4981 gtagaagatc cgttcgcgta tatgatgaaa catacaaaat cccacacgaa gaaatgctgg
     5041 aaatgatcaa agaagcgacg actgcacctt cttctgtcaa tatgaaacca tggcgctttg
     5101 tggtcgctga aagcgaggaa gcaaaagaaa tcttgcgtcc tttgatccgt ttcaatacga
     5161 gacaaaatga tacttcttct gcaatggtca tgatttttgg cgatatgcaa tgttacgaat
     5221 atggcgaaga aatctacaac caagcttacg aatcagggaa gatgccaaaa gaagtcagag
     5281 atcaacaatt agctgcgatc attccttatt acaagagttt ttcaagagaa gaaatgaacg
     5341 atgtagtcaa agtcgattct agcttagcgg ctatgcaatt catgttagtt gcgcgtgatc
     5401 atggctatga aacaaatcca attggtggat tcgaagcaga tcagttggca gaggcatttg
     5461 gtttggataa agaccgttat gttccagtta ttattttatc aataggaaaa gcggtagaag
     5521 aaggttacga gtctgttcgt ttagcaccag aaaagatcac tatgttcgaa taaaaggttt
     5581 tcagttacta ttcagtagct attgcttttt ttgaatcgtg tgtggtaacc taataaaaaa
     5641 ggaggtcgga aaaatgactg catcgatgaa attacgtttc gaacttttga ataacaacta
     5701 aataaattca aaagacatgt ataaacaaca atttcatgga tggagtatgt ccattttttc
     5761 gaacgacagt agaataggtg aaagctatga cagatttggt gatttagtca tagttatttc
     5821 gttcagagta ggaaaagatg tgatgcagtt tttttgcaaa cagatttttc tgctcttttt
     5881 tagtatcgat aattgttgtt ttacatgaaa taggagtgta aaacatatgg aaaatttagc
     5941 agtaaatata acaaatctac aagtcagctt tggcaaccag ctagaactat ctattgattc
     6001 tcttcgtgtc tatcagcaag atcgtatagg gatcatagga gaaaatggtg tcggtaaatc
     6061 gactttgctc aaactaatag ccggtgaact ttttcccgat catgggaaaa tccaaacaga
     6121 gatcaccttc aactacctgc ctcaattaac ctatctcgct gaagcaaagg acctaaattt
     6181 ggaattagcc agtcatttcc agttaagact ggaagaaact tcagagcgga aatggagcgg
     6241 aggggaagaa cgaaagatcg agttgatacg tcttctttct tcttatgaac aagggatgct
     6301 tctagacgag ccaacaaccc atctagatag aaaaagtatt gatcgactga ttgaagagct
     6361 tcgttattat tatggtacgc tggtttttgt tagtcatgat cgctattttc tagatgagct
     6421 agcatcgaaa atctgggaag taaaagacgg agaaatccga gagttttcgg ggaattatag
     6481 tgcctatctc actcaaaagg aattggagaa aaagactcag ctacgagaag cagagtcgat
     6541 catgaaagag aaaaaacgat tggaaaaatc gatccaagaa aagaaaaaac aagcggaaaa
     6601 gttggaaaaa gtgtccagta aaaagaaaaa gcaacaaatc agactggatc ggttgtcttc
     6661 ctctaaacaa aaagacagtg tacaaaaagc catccaaaag aatgcgaaaa cattagagag
     6721 aagactccaa aaaataggag aaacaaccaa gccgcagcag atgaaacaaa tccgttttcc
     6781 agtaccaaaa tctcttgaac tccacagccg ctatcccatc atgggacaaa atgtccaatt
     6841 ggaaagaagt gggagaacat tactggtaaa tggtgatttt cagttttctt taggtaaaaa
     6901 aatcgcgatt gtcggtgaaa atggttcagg taagacaacc ttattagaac atatccgcaa
     6961 acaaggagaa ggaatccttc tctctccgaa agtaagcttt caagtatatc agcaaaaggg
     7021 ttatcaaatg acatctgaag aatccatcat tcgttttgtc atgagacaaa cagagttttc
     7081 agaatcgctt gtccgtagtt tgctgaatca cttagggttt gctcaggaaa ctttgacgaa
     7141 accgttatgt acattaagtg ggggagaagc gacccgtctg acgattgctt tgctttttac
     7201 taagccaagt aatgtgttgc tgttagatga accgactaat tttattgata tggcaacgat
     7261 cgaagcttta gagaagctaa tgcaaatata tccgggaacg attttgttta cttctcatga
     7321 ttcttacttt gtcgagcgta cggctgatga agtttatgaa ataaaagggc agaaaataaa
     7381 aaaagtactt acgagaaatt tttaaatgtg ctacgatgat tttcgtgaca aatttaaagg
     7441 agtggtacag attgcctaca aataaaaaag aaggaataat ttttacaaca atgatgtgtt
     7501 ttttgatggt cttggggatg agcgcgtata atttatggct gcatcaagat cttcatttat
     7561 caagtttgtt gattggtcta gtgcctggat tcgtggtggc tttcattttg gatgtcttca
     7621 tcatcggtgt gctggcgaaa aaaattgcct ttaaactacc agtaaatcca gaaagcaagg
     7681 tccaaatgat cttaactatt tcatgcttga tggttttagg aatggtgaca tgtatgtctt
     7741 tattcggtgt gatcatggaa caaggaatac ctgatcaatt attctctgct tatatgaatg
     7801 cttggaaaat gaatgtgatt gccgctttac cgttgcagtt gatcatcgtt ggcccgcttt
     7861 caagaagtgt gctaaaagca attcaaagta atgaatgatg gaacactgtt atttgaaaaa
     7921 aggccttggc atgctttttg tcatagtctc ttgaatgcag ttctagaaaa atagtgatcc
     7981 gaacgatagg ttttggcctg tcgttcggat cgctttttct tttccaactg tattgctctt
     8041 ttattgaaga taatttgctc ttcaatagat atttctagaa aggttctgtt tatcccttac
     8101 tcaaaaaact gctgcatttg ttctcttact tgctgacacc attggagttc ctgctgacaa
     8161 agcataattc tgcgctggat cacaacataa cgtccaaatt cttgtttctt gtctagcgag
     8221 aggtcattca attttttttg atttttttgc atgattttgt tcagctgttg ttctctagtg
     8281 aatagcagtc cagtagctgt tgttttatcg agcgtcgaaa aagcatagat ttttgctaga
     8341 aattcatctt tttgcgtagg agcgggagtt tcctctttga tccattcctc tacaagagca
     8401 cgtccttcat cagtcaagtg gtagatcttt ttttgagagt gctcgtcttt gtccaaaacg
     8461 gtcacataac cctgctggtc taacttggca agcgttgtat agatctgact gtgatgggct
     8521 tcccaaaaaa ttgtgatcag ctgcttcaat tcatacccag aacgtggttt ggataaaagc
     8581 acgcataata atatataggc taaagtattc ataaaaatcc tacctctcgt cttattttct
     8641 ttattatatc ac
//
LOCUS       DAZKLA010000331         5592 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.guided.149, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000331 DAZKLA010000000
VERSION     DAZKLA010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.guided.149"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..829
                     /locus_tag="ACG85N_003045"
     CDS             137..829
                     /locus_tag="ACG85N_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0225709.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1156..1653
                     /gene="dfrG"
                     /locus_tag="ACG85N_003046"
     CDS             1156..1653
                     /gene="dfrG"
                     /locus_tag="ACG85N_003046"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0225710.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1725..3677)
                     /locus_tag="ACG85N_003047"
     CDS             complement(1725..3677)
                     /locus_tag="ACG85N_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0225711.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3684..3920)
                     /locus_tag="ACG85N_003048"
     CDS             complement(3684..3920)
                     /locus_tag="ACG85N_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225712.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4322..4612
                     /locus_tag="ACG85N_003049"
     CDS             4322..4612
                     /locus_tag="ACG85N_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225713.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(4884..5233)
                     /locus_tag="ACG85N_003050"
                     /pseudo
     CDS             complement(4884..5233)
                     /locus_tag="ACG85N_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            5326..>5592
                     /locus_tag="ACG85N_003051"
     CDS             5326..>5592
                     /locus_tag="ACG85N_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFQ0225714.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTI"
BASE COUNT         1900 a          901 c          973 g         1818 t
ORIGIN      
        1 gataaaaaag gaggattaaa tgagtatttg atagtattta tattaaaatt acatatgtaa
       61 tttaaaaacg cttgacattg tgtttgagat cacttacttt tagcatgtaa aagtattgga
      121 ttaaggagct atgcaaatga aaaagaaatt tattactggt gggattgtcg ttgcatttct
      181 atgtctaatt ggctacacag gcacgcggta cgcaaatgaa attattttgt ggggtggaga
      241 gacagatatt gatacaatca atgagaatct gacacagctt gatcatacat tgacagataa
      301 agagcaagcg attacagatc ttaccgctcg actagcacaa aaagaacaga cattgcaaga
      361 ttcaaaggca gatgtccaag aataccaaac aaaagtaaat gaattagaaa accaaaaaaa
      421 tcaatcggca gcagaaaaac aaaacttaga aagtcaatta gtcagcaagg atgcacaact
      481 acaaagcaaa caaaatgaaa tcaatgtcaa actagatgag atcaaccaaa aaaatgatga
      541 aatcaatcat ctgaatgcag attggacagc aagactacaa gaagcacaag caaaactttc
      601 agaattacaa aatactgtaa atggtttgaa tcaacaaatc gcaacactga cacaagaaaa
      661 aacaaatgta gaaactcagt taacagatac acaaaagaaa ttagacgaag caactgctga
      721 aaactcaagc ttgaaacaat atattcaaaa gttagaaaaa gcaaaagatg aagtggaaga
      781 tacagctgat aaatctcagc aaattgttga agaacatttg cgtaaataat ctttcttaac
      841 aagtaaacct aaattagtaa agaagcaaca gtaatctata ctaagtagtt cagttttgga
      901 gtacaaaatg acaaaatgta cttcaaaatg gactactttt ttcattttaa cttacatacc
      961 aaattacata caaaatgatt tataaaatgc aattgaaaat gataattgaa tgaaaatttg
     1021 tgattttgtg gtattatgat aatataactt atagaaaaaa ttgcttattc aactaacggg
     1081 caggataatg agaaaaggtt ttttgataga aataagaaat tagatggtaa attagttaaa
     1141 aaggctgtgt tataaatgaa agtttctttg attgctgcga tggataagaa tagagtgatt
     1201 ggcaaagaga atgacattcc ttggaggatt cccaaggact gggaatatgt taaaaatact
     1261 acaaagggac atccgataat attaggtagg aagaaccttg aatcaatcgg aagagcctta
     1321 cctgacagaa gaaatattat tctgacgaga gataaggggt ttacctttaa tggttgtgaa
     1381 attgttcatt caatagaaga tgtttttgag ttatgtaaaa acgaagaaga aatttttatt
     1441 ttcggaggag aacagattta taatttgttt ttcccttatg ttgagaaaat gtacatcaca
     1501 aaaatacatc atgaattcga aggagatact ttttttccag aagtgaatta tgaggaatgg
     1561 aatgaggtat ttgcccaaaa agggataaag aatgataaaa atccgtataa ctactatttt
     1621 catgtatatg aaagaaaaaa cttattgagt taacgggtgc gttaattgaa gaagaacagt
     1681 aaagataaag cacaagagct tgggattact ccaagctctt tttgtcattt gaaataaatt
     1741 atatctttcc aatcaaattg tttatctgta atccctaatc tttgagcagg tgtcaaaacc
     1801 tttttatccg ctgatttaaa agggcgacaa aagttgtagt aagttcttag aattgtaatc
     1861 gcaaattgag catacttcgg attgaagtta gcgtaaatat agctcttctt atcaccacga
     1921 gctgttgtta atggtctttc aagagatgat atataacgtc ttatctgttg aataaagcta
     1981 ttggtcgaat ggtcattaac atttaatagc atctttgcga tttcgtttgg ttccagagca
     2041 gataaatccg ttctacaatc aactgaataa aatcctttat ccttagtggc taatggatgt
     2101 ttgatagggt tatcggcata tttgcgatat ctttctccat ctttactaat agcctcttcg
     2161 tggaattgat gacttgttag taattcagtt agataatgag atgccaaagt ccgtagaaac
     2221 tttgttttga atcctctgat atctccccaa tcgagcagtt ctgcttttgc ttgtccgaat
     2281 tcttttaagc attgttttct gcttttcgtc ttgttaactt ggcaaataaa atgatgtgca
     2341 tcgctcaacc ttaattcttt cgtgaatact ctataaaagg cgtttctaat agagaagtca
     2401 tcatcactaa tcattcgcca ttcgtcggca ttaaccattt gtttaattaa ccaatagtga
     2461 gcagttgtag tataaggagc atcaacattt aaaccatcga caaatctact cctattagta
     2521 attttcccta attcgtgaag ataagcattc ctattttcgc tatcgttagc ggatggttct
     2581 tgtggataat aagaccaatc taacctatca tttttacgac taaaagtatt gagatggtct
     2641 tctttgaatt ttcttgtatc ttcatttaat tcatccattg aaatattcca atcataggca
     2701 acatcagaac ggaacacata tcttgataag acctcagcag atacaactat atacgtttgc
     2761 atattaaggt cttcaaaccc cgtatatttc tttgaacctt gtcctttctt acggacgtta
     2821 tttaggtagt agtgcatctt gtccgtatta agccacatct cgttaaattt ctttgtttga
     2881 aggggttgtg tctcatatcg ttctaaaaac tccaaacaac gcctatacag ccactccagc
     2941 ttatgatagt aggttccaac tccaattcct aaaatatcac aagtacgact gactgaaacc
     3001 cttccaacaa ccatcttagc gaacattgga agaatggtgt tcttttgttg gtgataagta
     3061 gtggtttctt ctctttttgg tagaacatta gtgaacttct tacacgcctt acattgatag
     3121 cgttgagatt tgcccctact tttccccctt ttgtaaaatt gcttcggctc attgaatggg
     3181 gttgattctt cttccgagca gccttcttta tgaaattgat aatctggctc aacatcttgg
     3241 atgctattga tttctataag gcgtttaatt tcttcaacaa cagaccaatt agataaaggg
     3301 gttactgtac agttttgcga tacacctctg ccaataggat tgtcattgca gtacatacct
     3361 ttatgacctt tatctttgga actaccagtc attttatatc gacttggttt cccttttaca
     3421 tctttgaatt tgtgctgttc tttcccaaaa ttacaacaaa acggattgat gcagtaatta
     3481 tattgaatgg gatattcaga accgttccat ttaaaagacg ttggcaagaa caacattttg
     3541 ttataccttg ttgcaaatcg ttttggagtt aataaatcat attgtttagc cctatctttt
     3601 aattcttcgt ttgaaatcgg aattttaaca tctacaattt catcttcctt cgtggctaat
     3661 cttttaagct tggtcattgt ctatcaaccc actctatctt tgatacccat tttttcaaca
     3721 atccgcttat tacttcgctt gttggcaatc cattcaataa acttcttatc gtctaaaatg
     3781 ttcaataaga attcatccac tgcttcgctt tcagtacgct ctgcatattc agcgtattgc
     3841 ttaactatca cccttacttg ctctgatatt tcccaatcca cagacttagc gttcttattt
     3901 tttggctcaa gaaattgcat aggaaaatgc accccttttt gcttagtaaa ttgtaaccat
     3961 tttaacacac caatttgcat tcctacaata attatttcac gttcgcattt ttatatacat
     4021 tttaacttac attttgtggt gtaaattaac aacaaacgtt gatttaacaa taaaaaacga
     4081 gtatcaaatt cagttgcaat ttgatactca tttaggttta cattttcact ttttgtactc
     4141 caaaactgaa ctacttaaat ctatactgct gcctcttttt tttagtacat gtccttcatc
     4201 ttcgcaggaa gtgccaatat gattttaaga aaggtaatag acaaaaagtc aaaaagataa
     4261 aacagagttt cttctttaca aagggaatgt atgttcgtat aatggtattg aggtgaaggt
     4321 tatggagaaa aaatatggaa cagtagataa gatccgtact ttgcaagtta gtgccagacc
     4381 gttgatccgt ttcagtttaa atggggtcaa ttgtttgatt gcttcccata gtttgaactt
     4441 cctagctgaa gtagacgaag ggatgaagtt agtcgttaca ggttattata ataatagaaa
     4501 gcaatttgtt gtgagatcct atcatgtgtt agggaaaccc aaaattgtgg tagaatatga
     4561 gaaaagtcta tacccaagaa aaaaggtccg acaagatgaa agaataaact agaaaacaag
     4621 cggctgagac aaaaatgtcc tagccgcttg ttttggaaag atggatgcag acgatgggat
     4681 ttgaacccac acgagcatac gctcacaaga acctcaatct atatataact acatgtattt
     4741 aagtatttgt gatgaatcac taaactttcc aaactcattt gagggatatt gcttaaagat
     4801 tgaacagatc aattcgaagc cctattagta ttcgctcaat ggggggatca gaggccataa
     4861 aaagagacta gtcattacta gtctcaggta gtggaattgt aggattttaa acgttcctgg
     4921 agagtaccgg tatgtaactc gaagaggtga ttatcatagt cgtaaaagta aattgaagaa
     4981 gcctcgcctt tcacacgaga acgtcctggt tcgacagtta aacctaacat ttggattttt
     5041 gagacaaacg aatctatatc ttgttcgtca attttaaaag caatatgatt gtatgtcttt
     5101 ggcagtttat tcgacgaatt ctgcattact gcaatccata ggtccttaac taagaaaaat
     5161 ttttctggat ataatgaaaa tttttttgtt gactatcgta gatttctttt gcatcaaata
     5221 aggtatttaa caattaagta tttaaaagat aacttttaag ttatctttaa gtaatgctta
     5281 aagctatgtt atactctatt aggaaatata agaaggagta taaaaatgaa caaacataat
     5341 agaaaaataa cgaataacaa agaactgatc ttgttactaa tagggatttg gggtatcttt
     5401 ttactcttta atatgtatat gccgactatg agagcggatg acgtagttta tgcaagtaga
     5461 ttagatgaac ttggctattt gggtgcttcg atagaacatt ataagacatg gagttcaaga
     5521 attattatag aactattctt aatgttcttt tctaaacact ttatgctatg gaaattatta
     5581 aattctacga tt
//
LOCUS       DAZKLA010000332         4862 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.guided.163, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000332 DAZKLA010000000
VERSION     DAZKLA010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.guided.163"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            260..1171
                     /locus_tag="ACG85N_003052"
     CDS             260..1171
                     /locus_tag="ACG85N_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0225715.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1324..1809
                     /gene="vanZ-A"
                     /locus_tag="ACG85N_003053"
     CDS             1324..1809
                     /gene="vanZ-A"
                     /locus_tag="ACG85N_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0225716.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            2328..2612
                     /locus_tag="ACG85N_003054"
     CDS             2328..2612
                     /locus_tag="ACG85N_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0225717.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            2978..3277
                     /locus_tag="ACG85N_003055"
     CDS             2978..3277
                     /locus_tag="ACG85N_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0225718.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            3320..4810
                     /locus_tag="ACG85N_003056"
     CDS             3320..4810
                     /locus_tag="ACG85N_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0225719.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
BASE COUNT         1801 a          715 c          893 g         1453 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc ccattgtcaa caggcagttc
       61 agcctcgtta aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata
      121 gtaaatccag tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca
      181 aacgagattg tgccgtttaa ggggaagatt ctagaaatat ttcatacttc caactatata
      241 gttaaggagg agactgaaaa tgaagaagtt gtttttttta ttgttattgt tattcttaat
      301 atacttaggt tatgactacg ttaatgaagc actgttttct caggaaaaag tcgaatttca
      361 aaattatgat caaaatccca aagaacattt agaaaatagt gggacttctg aaaataccca
      421 agagaaaaca attacagaag aacaggttta tcaaggaaat ctgctattaa tcaatagtaa
      481 atatcctgtt cgccaagaaa gtgtgaagtc agatatcgtg aatttatcta aacatgacga
      541 attaataaat ggatacgggt tgcttgatag taatatttat atgtcaaaag aaatagcaca
      601 aaaattttca gagatggtca atgatgctgt aaagggtggc gttagtcatt ttattattaa
      661 tagtggctat cgagactttg atgagcaaag tgtgctttac caagaaatgg gggctgagta
      721 tgccttacca gcaggttata gtgagcataa ttcaggttta tcactagatg taggatcaag
      781 cttgacgaaa atggaacgag cccctgaagg aaagtggata gaagaaaatg cttggaaata
      841 cgggttcatt ttacgttatc cagaggacaa aacagagtta acaggaattc aatatgaacc
      901 atggcatatt cgctatgttg gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt
      961 tctcgaggaa tatatggatt acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg
     1021 ggaaaaatat gagatctttt attatcctgt tactaaaaat accaccattc atgtgccgac
     1081 taatcttcgt tatgagatat caggaaacaa tatagacggt gtaattgtga cagtgtttcc
     1141 cggatcaaca catactaatt caaggaggta aggatggcgg aatgaaacca acgaaattaa
     1201 tgaacagcat tattgtacta gcacttttgg ggtaacgtta gctttttaat ttaaaaccca
     1261 cgttaactag gacattgcta tactaatgat acaacttaaa caaaagaatt agaggaaatt
     1321 atattgggaa aaatattatc tagaggattg ctagctttat atttagtgac actaatctgg
     1381 ttagtgttat tcaaattaca atacaatatt ttatcagtat ttaattatca tcaaagaagt
     1441 cttaacttga ctccatttac tgctactggg aatttcagag agatgataga taatgttata
     1501 atctttattc catttggctt gcttttgaat gtcaatttta aagaaatcgg atttttacct
     1561 aagtttgctt ttgtactggt tttaagtctt acttttgaaa taattcaatt tatcttcgct
     1621 attggagcga cagacataac agatgtaatt acaaatactg ttggaggctt tcttggactg
     1681 aaattatatg gtttaagcaa taagcatatg aatcaaaaaa aattagacag agttattatt
     1741 tttgtaggta tacttttgct cgtattattg ctcgtttacc gtacccattt aagaataaat
     1801 tacgtgtaag atgtctaaat caagcaatct gatctttcat acacataaag atattgaatg
     1861 aattggatta gatggaaaac gggatgtggg gaaactcgcc cgtaggtgtg aagtgagggg
     1921 aaaaccggtg ataaagtaaa aagcttacct aacactatag taacaaagaa agcccaatta
     1981 tcaattttag tgctgaggaa ttggtctctt taataaattt ccttaacgtt gtaaatccgc
     2041 attttcctga cggtaccccc actagcagcc atgctttttt tgaatctttg taggacgttt
     2101 taaagagaat tacccacttt aaaattaaac atgcgcttag aatcgcttta ggaaacacga
     2161 tccagtccaa taatcgtcga taaaaacttt tgaaaaaggt tggtgaaatt acctactttt
     2221 ggaataatca caaatcacaa gtgattaatc acttgtttat taagatatta aaagctataa
     2281 tttaaataaa gcgtgaattt tattacacaa aaagaggggg gagaaacttg gaactagcat
     2341 ttagagaaag cttaaaaaag atgagaggta ccaaatcaaa agaaaaattc tcccaagaat
     2401 tagaaatgag tagatcaaat tattcacgaa tagaatcagg aaaatcagat ccaaccataa
     2461 aaacactaga acaaattgca aagttaacta actcaacgct agtagtggat ttaatcccaa
     2521 atgagccaac agaaccagaa ccagaaacag aatcagaaca agtaacattg gatttagaaa
     2581 tggaagaaga aaaaagcaat gacttcgtgt gaataatgca cgaaatcgtt gcttattttt
     2641 tttaaaagcg gtatactaga tataacgaaa caacgaactg aatagaaacg aaaaaagagc
     2701 catgacacat ttataaaatg tttgacgaca ttttataaat gcatagcccg ataagattgc
     2761 caaaccaacg cttatcagtt agtcagatga actcttccct cgtaagaagt tatttaatta
     2821 actttgtttg aagacggtat ataaccgtac tatcattata tagggaaatc agagagtttt
     2881 caagtatcta agctactgaa tttaagaatt gttaagcaat caatcggaaa tcgtttgatt
     2941 gctttttttg tattcattta tagaaggtgg agtttgtatg aatcatgatg aatgtaaaac
     3001 ttatataaaa aatagtttat tggagataag aaaattagca aatatctata cactagaaac
     3061 gtttaagaaa gagttagaaa agagaaatat ctacttagaa acaaaatcag ataagtattt
     3121 ttcttcggag ggggaagatt atatatataa gttaatagaa aataacaaaa taatttattc
     3181 gattagtgga aaaaaattga cttataaagg aaaaaaatct ttttcaaaac atgcaatatt
     3241 gaaacagttg aatgaaaaag caaaccaagt taattaaaca acctatttta taggatttat
     3301 aggaaaggag aacagctgaa tgaatatccc ttttgttgta gaaactgtgc ttcatgacgg
     3361 cttgttaaag tacaaattta aaaatagtaa aattcgctca atcactacca agccaggtaa
     3421 aagcaaaggg gctatttttg cgtatcgctc aaaatcaagc atgattggcg gtcgtggtgt
     3481 tgttctgact tccgaggaag cgattcaaga aaatcaagat acatttacac attggacacc
     3541 caacgtttat cgttatggaa cgtatgcaga cgaaaaccgt tcatacacga aaggacattc
     3601 tgaaaacaat ttaagacaaa tcaatacctt ctttattgat tttgatattc acacggcaaa
     3661 agaaactatt tcagcaagcg atattttaac aaccgctatt gatttaggtt ttatgcctac
     3721 tatgattatc aaatctgata aaggttatca agcatatttt gttttagaaa cgccagtcta
     3781 tgtgacttca aaatcagaat ttaaatctgt caaagcagcc aaaataattt cgcaaaatat
     3841 ccgagaatat tttggaaagt ctttgccagt tgatctaacg tgtaatcatt ttggtattgc
     3901 tcgcatacca agaacggaca atgtagaatt ttttgatcct aattaccgtt attctttcaa
     3961 agaatggcaa gattggtctt tcaaacaaac agataataag ggctttactc gttcaagtct
     4021 aacggtttta agcggtacag aaggcaaaaa acaagtagat gaaccctggt ttaatctctt
     4081 attgcacgaa acgaaatttt caggagaaaa gggtttaata gggcgtaata acgtcatgtt
     4141 taccctctct ttagcctact ttagttcagg ctattcaatc gaaacgtgcg aatataatat
     4201 gtttgagttt aataatcgat tagatcaacc cttagaagaa aaagaagtaa tcaaaattgt
     4261 tagaagtgcc tattcagaaa actatcaagg ggctaatagg gaatacatta ccattctttg
     4321 caaagcttgg gtatcaagtg atttaaccag taaagattta tttgtccgtc aagggtggtt
     4381 taaattcaag aaaaaaagaa gcgaacgtca acgtgttcat ttgtcagaat ggaaagaaga
     4441 tttaatggct tatattagcg aaaaaagcga tgtatacaag ccttatttag tgacgaccaa
     4501 aaaagagatt agagaagtgc taggcattcc tgaacggaca ttagataaat tgctgaaggt
     4561 actgaaggcg aatcaggaaa ttttctttaa gattaaacca ggaagaaatg gtggcattca
     4621 acttgctagt gttaaatcat tgttgctatc gatcattaaa gtaaaaaaag aagaaaaaga
     4681 aagctatata aaggcgctga caaattcttt tgacttagag catacattca ttcaagagac
     4741 tttaaacaag ctagcagaac gccctaaaac ggacacacaa ctcgatttgt ttagctatga
     4801 tacaggctga aaataaaacc cgcactatgc cattacattt atatctatga tacgtgtttg
     4861 tt
//
LOCUS       DAZKLA010000333         3306 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.guided.192, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000333 DAZKLA010000000
VERSION     DAZKLA010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.guided.192"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..998)
                     /locus_tag="ACG85N_003057"
                     /pseudo
     CDS             complement(1..998)
                     /locus_tag="ACG85N_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            1489..2427
                     /locus_tag="ACG85N_003058"
     CDS             1489..2427
                     /locus_tag="ACG85N_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0225720.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            2557..3204
                     /locus_tag="ACG85N_003059"
     CDS             2557..3204
                     /locus_tag="ACG85N_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HFQ0225721.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
BASE COUNT         1112 a          483 c          549 g         1162 t
ORIGIN      
        1 aatacaatat tgttgccaca cttaggacat tttcctacaa tacttttttc tgcttctttt
       61 tctttctgtt cctggtaatc agaaaaattt agtttttcta tatcgttagg tacagcttcc
      121 agtaaatgaa caatgaattt tttgatattc gtaataaagt tctcttgatt gccttctctt
      181 ttaccgattt tttttaaata cgtttcccat ttagccgtca tttcagcact cgttaaaagg
      241 tgctgacttt caactgcctg gcacaataat tttccttttt cagttacaac aagcttattc
      301 ttaatcactt ggatatattc tttttgtttt aacgcttcaa taatacttgc tcttgtcgct
      361 tctgttccaa tcccctcaac ttcttgtaaa atcttgattg cttcttcatc atcaaccgtt
      421 ttattcgccg ttttcatagc agttaataat gtaccctctg taaaagcttt tggcggttgc
      481 gtttcttttt caacactctt aacaccaact tcggcacgtt cgccaatgat aacgagtggt
      541 aacgtttgaa cgtcctcttt ttcttctttg gtttgttgtt tgaaaagaat tttccaccct
      601 tcttgctttg gtgtctttcc gatcgattga aacagcaaat cagctacttt cgtttgtatc
      661 ttggtttctt catacaagta atcaggtaaa aacatggcaa cggtcgtttt aacgactaaa
      721 gcataaattt ttcgttgtaa atcgtccatt ttcgctaatg cagattcggt aggtacttgt
      781 tttgttggga taatggcgtg gtgttcctgt accttactac cgtccacata acgctttcta
      841 ggctctgttt gaaccatttc aagatcaagt cctaaaaaac cgctatattt gccaaaattc
      901 gcttttaaat aagcaaattc gttctctgta ataaatggtg tatctgttct tggataactc
      961 aataattttg cttcatacag tccttgcata gcttttaaac gtaaaatttt gcgttctaag
     1021 acgttttaaa tatatctgcg tatattttta tgtgttttat cttttcgtcg gcaaatgcgt
     1081 taaaatttgc gaaataaaat agctcaaggg cgaacgcagt gagtgcattt acccttgatc
     1141 tatttttttg agtaaattag cttttgaaac cgacggaaag gaaacacaaa acttttcaga
     1201 ggcaaggggt ttttaagggg cgcttttagc cccttgaatt tatttgcttt aagatatatt
     1261 tagttttaaa atagttttaa cgaaaaaaag acataaaaaa agacgagcaa cgctaccaac
     1321 gttctcgccc ggaataacaa accacacttg ttattcattc gatgattagt atatcaattt
     1381 ttgattttga atacaaaaca aaatggacga atggatacag gcgaaattta ttttgcgatt
     1441 ccactcgtcc ttttttcgtt ccaaaaatta ttttaggagc gataaaaaat gaagaaaatt
     1501 gatgaaaaat tagctgcaga aatctcacta aaaaaaccgg ccgtctctaa tagccggtta
     1561 agtaatagcc ggttaagtgg tcaaactttg ggaaaatctc aacccgcatt aagttttgat
     1621 gccatgacaa tcgttggaaa tttgaacaaa actaatgcta aaaagctatc tgactttatg
     1681 agtgtagagc cacaaatacg actttgggat atacttcaaa caaagtttaa agctaaggca
     1741 cttcaagaaa aagtttatat cgaatatgac aaagtaaaag cagatacttg ggatagacgt
     1801 aatatgcgtg ttgaatttaa tcccaataaa ctcacacatg aagaaatgat ttggttaaaa
     1861 caaaatatta tcgactacat ggaagatgac ggttttacaa gattagactt agcttttgat
     1921 tttgaagatg atttgagcga ttactatgca atgactgata aagcagttaa gaaaactgtt
     1981 ttttatggtc gtaatggcaa gccagaaaca aaatattttg gtgtccgtga tagtgataga
     2041 tttattagaa tttataataa aaaacaagaa cgtaaagata acgcagatgt tgaagttgtg
     2101 tctgaacatt tatggcgtgt agaagttgaa ttaaaaagag atatggttga ttactggaat
     2161 gattgtttta atgatttaca catcttgaaa cctgcgtggg ctactttaga aaaaattaat
     2221 gagcaagcta tggtttatac tttgttgcat gaagaaagta tgtggggaaa gctaagtaag
     2281 aatactaaga ctaaatttaa aaaattgatt agagaaatat ctccaattga tttaacggaa
     2341 ttaatgaaat cgactttaaa agcgaacgaa aaacaattgc aaaagcagat tgatttttgg
     2401 caacgtgaat ttaggttttg gaagtaaaat aagttttatt tgataaaaat tgctaattca
     2461 gtataattaa tatttacgag gtgacataac gtatgaaaaa atcagaggat tattcctcct
     2521 aaatataaaa atttaaaatt taggaggaag ttatatatga cttttaatat tattgaatta
     2581 gaaaattggg atagaaaaga atattttgaa cactatttta atcagcaaac tacttatagc
     2641 attactaaag aaattgatat tactttgttt aaagatatga taaaaaagaa aggatatgaa
     2701 atttatccct ctttaattta tgcaattatg gaagttgtaa ataaaaataa agtgtttaga
     2761 acaggaatta atagtgagaa taaattaggt tattgggata agttaaatcc tttgtataca
     2821 gtttttaata agcaaactga aaaatttact aacatttgga ctgaatctga taaaaacttc
     2881 atttcttttt ataataatta taaaaatgac ttgcttgaat ataaagataa agaagaaatg
     2941 tttcctaaaa aaccgatacc tgaaaacacc ataccgattt caatgattcc ttggattgat
     3001 tttagttcat ttaatttaaa tattggtaac aatagcagct ttttattgcc tattattacg
     3061 ataggtaaat tttatagtga gaataataaa atttatatac cagttgctct gcaacttcat
     3121 cattctgtat gtgatggtta ccatgcttca ctatttatga atgaatttca agatataatt
     3181 catagggtag atgattggat ttagttttta gattttgaaa gtgaatttaa ttttatacac
     3241 gtaagtgatc ataaaattta tgaacgtata acaaccacat tttttggttg cttgtggttt
     3301 tgattt
//
LOCUS       DAZKLA010000334         3083 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.guided.197, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000334 DAZKLA010000000
VERSION     DAZKLA010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.guided.197"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..877
                     /locus_tag="ACG85N_003060"
     CDS             8..877
                     /locus_tag="ACG85N_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0225722.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            858..1592
                     /locus_tag="ACG85N_003061"
     CDS             858..1592
                     /locus_tag="ACG85N_003061"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0225723.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1625..2533
                     /locus_tag="ACG85N_003062"
     CDS             1625..2533
                     /locus_tag="ACG85N_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0225724.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2530..3010
                     /locus_tag="ACG85N_003063"
                     /pseudo
     CDS             2530..3010
                     /locus_tag="ACG85N_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1073 a          437 c          675 g          898 t
ORIGIN      
        1 tgtttatatg gtaggtaatg ttattcaaat agtaacagag aaattatcat ctttgccttt
       61 tatagaaggc atcgtattag ggggctcacg tgcaagaggc acccatacag aaaattctga
      121 tatagatatc ggaatctatt acaattcaga ctcatttgac ctgacagcga ttaatcaaat
      181 tgctacagaa ttggatgata aaaatagaaa caaccttgtt gtgcctcccg gagcgtgggg
      241 tgattggatt aatggcggcg gatggttatt tataaatggg tatcatgttg acttgatttt
      301 acgtgatata aaacgggtag aacaaataat caaagatacg gagcaaggaa ttgttactgc
      361 caattatcag accgggcatc cccatggtta tataagtgca atgtatcgtg gagaattagc
      421 gattagtaaa atactatatg ctaagaacga aagcttatgc gaattaaaaa agcaggcaga
      481 aatttaccct actgctctaa agaaaagctt gatgaacttt tttatatttg aagctgaatt
      541 ctctttaatg tttgtaaaag caaatgcggg ggtagaggat aaatattata ttgcaggcca
      601 tgtttttcgt attatttcat gcttaaatca agtactattt gcatgtaata atgcttattg
      661 cattaacgaa aagaaagcta taaaactgct tgaaactttt gaatataaac ctgaaaaata
      721 tgccgaaaga gttaatcata tttttgaagt actcggtctt tctctttttg aatgctacga
      781 tatgaccgag aaactttata aagaagtgaa tgaaattgta tcggagataa ataacttttt
      841 aaacgaggag agttcagatg aaagaaaaca aatatgatga taatatattt tttcaaaaat
      901 acagtcaaat gagtcgctcg cagaaaggac tggctggtgc gggagaatgg gagactttga
      961 aaaagatgct acctgatttt aagggtaagc gtgtgcttga tttaggatgc ggctatggat
     1021 ggcactgtat atatgcgatg gaaaacggtg cttcctctgt agtaggtgtt gatatttctc
     1081 ataaaatgct cgaagtagca aaaggaaaaa cccattttcc acagattgaa tatgaatgct
     1141 gtgccataga agatgtggat ttcccagagg agagctttga tgtaatacta agttcgcttg
     1201 cgtttcatta tgtagcagac tatgagaatt taataaaaaa gatatatagg atgctgaagg
     1261 ctggtggcaa tttagttttt acagttgaac atcctgtttt tactgctcat ggaacacaag
     1321 actggtatta taacgaaaaa ggagaaatac tgcatttccc ggtggacaat tattattatg
     1381 agggcaaacg gacagctatg tttttggaag aaaaggttac aaaatatcat agaacactga
     1441 ccacatatct aaatacactg ctttcaaata gttttataat aaatcagatt gtggagccac
     1501 agccgccaga gaacatgatg gatattccgg ggatggcgga tgaaatgcga cgcccaatga
     1561 tgctgattgt atcggcaaaa aagaagatgt aataatatag aaaaaataaa cgaggagtat
     1621 gtaaatgaga tcagaaaaag aaatgatgga tttagtactt tctttagcag aacaggatga
     1681 acgtattcga attgtgaccc ttgaggggtc acgcgcaaat attaatatac ctaaagatga
     1741 atttcaggat tatgatatta catattttgt aagtgatata gaaccgttta tatctaatga
     1801 tgactggctt aatcaatttg ggaatataat aatgatgcaa aagccggagg atatggaatt
     1861 attcccacct gaagaaaagg gattttccta tcttatgcta tttgatgatt acaataaaat
     1921 tgatcttacc ttattgccct tggaagagtt agataattac ctaaagggcg ataaattaat
     1981 aaaggttcta attgataaag attgtagaat taaaagggac atagttccga ctgatataga
     2041 ttatcatgta agaaagccaa gcgcaaggga gtatgatgat tgctgcaatg aattttggaa
     2101 tgtaacacct tatgttatta aaggattgtg ccgtaaggaa attttatttg ctattgatca
     2161 ttttaatcag attgttcgcc atgagctgct gagaatgata tcatggaagg tcggcatcga
     2221 aacaggcttt aaattaagtg taggcaagaa ctataagttt attgaaaggt atatatccga
     2281 ggatttgtgg gagaaacttt tgtccaccta ccggatggat tcctatgaaa acatatggga
     2341 agcattattt ctatgccatc aattgttcag ggcggtatcc ggtgaggtgg cggaaaggct
     2401 tcattatgcc tatccggagt atgataggaa tataacaaaa tataccaggg acatgtataa
     2461 aaaatacact ggtaaaaccg gctgcctgga tagcacatat gccgctgata tagaagagag
     2521 gcgggaacag tgattacaga aatgaaagca gggcacctga aagatatcga taaacccagc
     2581 gaaccatttg aggtgatagg taagattata ccgaggtatg aaaacgagaa ttggaccttt
     2641 acagaattac tctatgaagc gccatattta aaaagctacc aagacgaaga ggatgaagag
     2701 gatgaggagg cagattgcct tgaatatatt gacaatactg ataagataat atatctttta
     2761 tatagaagat atcgccgtat gtaaggattt cagggggcaa ggcataggca gcgcgcttat
     2821 caatatatct atagaatggg caaagcataa aaacttgcat ggactaatgc ttgaaaccca
     2881 ggacaataac cttatagctt gtaaattcta tcataattgt ggtttcaaaa tcggctccgt
     2941 cgatactatg ttatacgcca actttgaaaa caactttgaa aaagctgttt tctggtattt
     3001 aaggttttag aatgcaagga acagtgaatt ggagttcgtc ttgtggttct gttgcaaagt
     3061 tttaaataaa gaataaaatc cct
//
LOCUS       DAZKLA010000335         2922 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.guided.201, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000335 DAZKLA010000000
VERSION     DAZKLA010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.guided.201"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            252..1220
                     /locus_tag="ACG85N_003064"
     CDS             252..1220
                     /locus_tag="ACG85N_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0225725.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1213..2244
                     /gene="vanA"
                     /locus_tag="ACG85N_003065"
     CDS             1213..2244
                     /gene="vanA"
                     /locus_tag="ACG85N_003065"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0225726.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2250..2858
                     /locus_tag="ACG85N_003066"
     CDS             2250..2858
                     /locus_tag="ACG85N_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0225727.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          867 a          558 c          712 g          785 t
ORIGIN      
        1 agcgatgcca gacttggttg ataaaaggag gtcctaagag atgtatataa ttttttagga
       61 aaatctcaag gttatcttta ctttttctta ggaaattaac aatttaatat taagaaacgg
      121 ctcgttctta cacggtagac ttaataccgt aagaacgagc cgttttcgtt cttcagagaa
      181 agatttgaca agattaccat tggcatcccc gttttatttg gtgcctttca cagaaagggt
      241 tggtcttaat tatgaataac atcggcatta ctgtttatgg atgtgagcag gatgaggcag
      301 atgcattcca tgctctttcg cctcgctttg gcgttatggc aacgataatt aacgccaacg
      361 tgtcggaatc caacgccaaa tccgcgcctt tcaatcaatg tatcagtgtg ggacataaat
      421 cagagatttc cgcctctatt cttcttgcgc tgaagagagc cggtgtgaaa tatatttcta
      481 cccgaagcat cggctgcaat catatagata caactgctgc taagagaatg ggcatcactg
      541 tcgacaatgt ggcgtactcg ccggatagcg ttgccgatta tactatgatg ctaattctta
      601 tggcagtacg caacgtaaaa tcgattgtgc gctctgtgga aaaacatgat ttcaggttgg
      661 acagcgaccg tggcaaggta ctcagcgaca tgacagttgg tgtggtggga acgggccaga
      721 taggcaaagc ggttattgag cggctgcgag gatttggatg taaagtgttg gcttatagtc
      781 gcagccgaag tatagaggta aactatgtac cgtttgatga gttgctgcaa aatagcgata
      841 tcgttacgct tcatgtgccg ctcaatacgg atacgcacta tattatcagc cacgaacaaa
      901 tacagagaat gaagcaagga gcatttctta tcaatactgg gcgcggtcca cttgtagata
      961 cctatgagtt ggttaaagca ttagaaaacg ggaaactggg cggtgccgca ttggatgtat
     1021 tggaaggaga ggaagagttt ttctactctg attgcaccca aaaaccaatt gataatcaat
     1081 ttttacttaa acttcaaaga atgcctaacg tgataatcac accgcatacg gcctattata
     1141 ccgagcaagc gttgcgtgat accgttgaaa aaaccattaa aaactgtttg gattttgaaa
     1201 ggagacagga gcatgaatag aataaaagtt gcaatactgt ttgggggttg ctcagaggag
     1261 catgacgtat cggtaaaatc tgcaatagag atagccgcta acattaataa agaaaaatac
     1321 gagccgttat acattggaat tacgaaatct ggtgtatgga aaatgtgcga aaaaccttgc
     1381 gcggaatggg aaaacgacaa ttgctattca gctgtactct cgccggataa aaaaatgcac
     1441 ggattacttg ttaaaaagaa ccatgaatat gaaatcaacc atgttgatgt agcattttca
     1501 gctttgcatg gcaagtcagg tgaagatgga tccatacaag gtctgtttga attgtccggt
     1561 atcccttttg taggctgcga tattcaaagc tcagcaattt gtatggacaa atcgttgaca
     1621 tacatcgttg cgaaaaatgc tgggatagct actcccgcct tttgggttat taataaagat
     1681 gataggccgg tggcagctac gtttacctat cctgtttttg ttaagccggc gcgttcaggc
     1741 tcatccttcg gtgtgaaaaa agtcaatagc gcggacgaat tggactacgc aattgaatcg
     1801 gcaagacaat atgacagcaa aatcttaatt gagcaggctg tttcgggctg tgaggtcggt
     1861 tgtgcggtat tgggaaacag tgccgcgtta gttgttggcg aggtggacca aatcaggctg
     1921 cagtacggaa tctttcgtat tcatcaggaa gtcgagccgg aaaaaggctc tgaaaacgca
     1981 gttataaccg ttcccgcaga cctttcagca gaggagcgag gacggataca ggaaacggca
     2041 aaaaaaatat ataaagcgct cggctgtaga ggtctagccc gtgtggatat gtttttacaa
     2101 gataacggcc gcattgtact gaacgaagtc aatactctgc ccggtttcac gtcatacagt
     2161 cgttatcccc gtatgatggc cgctgcaggt attgcacttc ccgaactgat tgaccgcttg
     2221 atcgtattag cgttaaaggg gtgataagca tggaaatagg atttactttt ttagatgaaa
     2281 tagtacacgg tgttcgttgg gacgctaaat atgccacttg ggataatttc accggaaaac
     2341 cggttgacgg ttatgaagta aatcgcattg tagggacata cgagttggct gaatcgcttt
     2401 tgaaggcaaa agaactggct gctacccaag ggtacggatt gcttctatgg gacggttacc
     2461 gtcctaagcg tgctgtaaac tgttttatgc aatgggctgc acagccggaa aataacctga
     2521 caaaggaaag ttattatccc aatattgacc gaactgagat gatttcaaaa ggatacgtgg
     2581 cttcaaaatc aagccatagc cgcggcagtg ccattgatct tacgctttat cgattagaca
     2641 cgggtgagct tgtaccaatg gggagccgat ttgattttat ggatgaacgc tctcatcatg
     2701 cggcaaatgg aatatcatgc aatgaagcgc aaaatcgcag acgtttgcgc tccatcatgg
     2761 aaaacagtgg gtttgaagca tatagcctcg aatggtggca ctatgtatta agagacgaac
     2821 cataccccaa tagctatttt gatttccccg ttaaataaac ttttaaccgt tgcacggaca
     2881 aacggttctg ttgcaaagtt ttaaataaag aataaaatcc ct
//
LOCUS       DAZKLA010000336         2042 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.guided.220, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000336 DAZKLA010000000
VERSION     DAZKLA010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.guided.220"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            92..139
                     /locus_tag="ACG85N_003067"
                     /pseudo
     CDS             92..139
                     /locus_tag="ACG85N_003067"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            155..>2042
                     /gene="tet(M)"
                     /locus_tag="ACG85N_003068"
     CDS             155..>2042
                     /gene="tet(M)"
                     /locus_tag="ACG85N_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFQ0225728.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRID"
BASE COUNT          692 a          320 c          417 g          613 t
ORIGIN      
        1 agtttaagaa tacctttatc atgtgattct aaagtatccg gagaatatct gtatgctttg
       61 tatgcctatg gttatgcata aaaatcccag tgataaaagt atttatcact gggattttta
      121 tgcccttttg ggtttttgaa tggaggaaaa tcacatgaaa attattaata ttggagtttt
      181 agctcatgtt gatgcaggaa aaactacctt aacagaaagc ttattatata acagtggagc
      241 gattacagaa ttaggaagcg tggacaaagg tacaacgagg acggataata cgcttttaga
      301 acgtcagaga ggaattacaa ttcagacagg aataacctct tttcagtggg aaaatacgaa
      361 ggtgaacatc atagacacgc caggacatat ggatttctta gcagaagtat atcgttcatt
      421 atcagtttta gatggggcaa ttctactgat ttctgcaaaa gatggcgtac aagcacaaac
      481 tcgtatatta tttcatgcac ttaggaaaat ggggattccc acaatctttt ttatcaataa
      541 gattgaccaa aatggaattg atttatcaac ggtttatcag gatattaaag agaaactttc
      601 tgccgaaatt gtaatcaaac agaaggtaga actgtatcct aatatgtgtg tgacgaactt
      661 taccgaatct gaacaatggg atacggtaat agagggaaac gatgaccttt tagagaaata
      721 tatgtccggt aaatcattag aagcattgga actcgaacaa gaggaaagca taagatttca
      781 gaattgttct ctgttccctc tttatcatgg aagtgcaaaa agtaatatag ggattgataa
      841 ccttatagaa gttattacta ataaatttta ttcatcaaca catcgaggtc cgtctgaact
      901 ttgcggaaat gttttcaaaa ttgaatatac aaaaaaaaga caacgtcttg catatatacg
      961 cctttatagt ggagtactac atttacgaga ttcggttaga gtatcagaaa aagaaaaaat
     1021 aaaagttaca gaaatgtata cttcaataaa tggtgaatta tgtaagattg atagagctta
     1081 ttctggagaa attgttattt tgcaaaatga gtttttgaag ttaaatagtg ttcttggaga
     1141 tacaaaacta ttgccacaga gaaaaaagat tgaaaatccg caccctctac tacaaacaac
     1201 tgttgaaccg agtaaacctg aacagagaga aatgttgctt gatgcccttt tggaaatctc
     1261 agatagtgat ccgcttctac gatattacgt ggattctacg acacatgaaa ttatactttc
     1321 tttcttaggg aaagtacaaa tggaagtgat tagtgcactg ttgcaagaaa agtatcatgt
     1381 ggagatagaa ctaaaagagc ctacagtcat ttatatggag agaccgttaa aaaatgcaga
     1441 atataccatt cacatcgaag tgccgccaaa tcctttctgg gcttccattg gtttatctgt
     1501 atcaccgctt ccgttgggaa gtggaatgca gtatgagagc tcggtttctc ttggatactt
     1561 aaatcaatcg tttcaaaatg cagttatgga ggggatacgc tatggctgtg aacaaggatt
     1621 gtatggttgg aatgtgacgg actgtaaaat ctgttttaag tatggcttat actatagccc
     1681 tgttagtacc ccagcagatt ttcggatgct tgctcctatt gtattggaac aagtcttaaa
     1741 aaaagctgga acagaattgt tagagccata tcttagtttt aaaatttatg cgccacagga
     1801 atatctttca cgagcataca acgatgctcc taaatattgt gcgaacatcg tagacactca
     1861 attgaaaaat aatgaggtca ttcttagtgg agaaatccct gctcggtgta ttcaagaata
     1921 tcgtagtgat ttaactttct ttacaaatgg acgtagtgtt tgtttaacag agttaaaagg
     1981 gtaccatgtt actaccggtg aacctgtttg ccagccccgt cgtccaaata gtcggataga
     2041 ta
//
LOCUS       DAZKLA010000337         1920 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.guided.226, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000337 DAZKLA010000000
VERSION     DAZKLA010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.guided.226"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..785
                     /gene="vanR-A"
                     /locus_tag="ACG85N_003069"
     CDS             90..785
                     /gene="vanR-A"
                     /locus_tag="ACG85N_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0225729.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            763..1917
                     /locus_tag="ACG85N_003070"
     CDS             763..1917
                     /locus_tag="ACG85N_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0225730.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          627 a          369 c          420 g          504 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcct ataatctgat aaattattaa
       61 gggcaaactt atgtgaaagg gtgataacta tgagcgataa aatacttatt gtggatgatg
      121 aacatgaaat tgccgatttg gttgaattat acttaaaaaa cgagaattat acggttttca
      181 aatactatac cgccaaagaa gcattggaat gtatagacaa gtctgagatt gaccttgcca
      241 tattggacat catgcttccc ggcacaagcg gccttactat ctgtcaaaaa ataagggaca
      301 agcacaccta tccgattatc atgctgaccg ggaaagatac agaggtagat aaaattacag
      361 ggttaacaat cggcgcggat gattatataa cgaagccctt tcgcccactg gagttaattg
      421 ctcgggtaaa ggcccagttg cgccgataca aaaaattcag tggagtaaag gagcagaacg
      481 aaaatgttat cgtccactcc ggccttgtca ttaatgttaa cacccatgag tgttatctga
      541 acgagaagca gttatccctt actcccaccg agttttcaat actgcgaatc ctctgtgaaa
      601 acaaggggaa tgtggttagc tccgagctgc tatttcatga gatatggggc gacgaatatt
      661 tcagcaagag caacaacacc atcaccgtgc atatccggca tttgcgcgaa aaaatgaacg
      721 acaccattga taatccgaaa tatataaaaa cggtatgggg ggttggttat aaaattgaaa
      781 aataaaaaaa acgactattc caaactagaa cgaaaacttt acatgtatat cgttgcaatt
      841 gttgtggtag caattgtatt cgtgttgtat attcgttcaa tgatccgagg gaaacttggg
      901 gattggatct taagtatttt ggaaaacaaa tatgacttaa atcacctgga cgcgatgaaa
      961 ttatatcaat attccatacg gaacaatata gatatcttta tttatgtggc gattgtcatt
     1021 agtattctta ttctatgtcg cgtcatgctt tcaaaattcg caaaatactt tgacgagata
     1081 aataccggca ttgatgtact tattcagaac gaagataaac aaattgagct ttctgcggaa
     1141 atggatgtta tggaacaaaa gctcaacaca ttaaaacgga ctctggaaaa gcgagagcag
     1201 gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta tgtacttggc gcacgatatt
     1261 aaaacgcccc ttacatccat tatcggttat ttgagcctgc ttgacgaggc tccagacatg
     1321 ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg acaaagcgta tcgactcgaa
     1381 cagctaatcg acgagttttt tgagattaca cggtataacc tacaaacgat aacgctaaca
     1441 aaaacgcaca tagacctata ctatatgctg gtgcagatga ccgatgaatt ttatcctcag
     1501 ctttccgcac atggaaaaca ggcggttatt cacgcccccg aggatctgac cgtgtccggc
     1561 gaccctgata aactcgcgag agtctttaac aacattttga aaaacgccgc tgcatacagt
     1621 gaggataaca gcatcattga cattaccgcg ggcctctccg gggatgtggt gtcaatcgaa
     1681 ttcaagaaca ctggaagcat cccaaaagat aagctagctg ccatatttga aaagttctat
     1741 aggctggaca atgctcgttc ttccgatacg ggtggcgcgg gacttggatt ggcgattgca
     1801 aaagaaatta ttgttcagca tggagggcag atttacgcgg aaagcaatga taactatacg
     1861 acgtttaggg tagagcttcc agcgatgcca gacttggttg ataaaaggag gtcctaagag
//
LOCUS       DAZKLA010000338         1148 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.guided.274, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000338 DAZKLA010000000
VERSION     DAZKLA010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.guided.274"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..76
                     /locus_tag="ACG85N_003071"
     CDS             26..76
                     /locus_tag="ACG85N_003071"
                     /inference="COORDINATES: protein motif:HMM:NF018061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFQ0225731.1"
                     /translation="MWTLSKQTKNSDYADK"
     gene            201..938
                     /gene="erm(B)"
                     /locus_tag="ACG85N_003072"
     CDS             201..938
                     /gene="erm(B)"
                     /locus_tag="ACG85N_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0225732.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          421 a          199 c          185 g          343 t
ORIGIN      
        1 atggcctttt atcttttaca caattatatg gactttatcg aaacagacta aaaacagtga
       61 ttacgcagat aaataaatac gttagattaa ttcctaccag tgactaatct tatgactttt
      121 taaacagata actaaaatta caaacaaatc gtttaacttc tgtatttgtt tatagatgta
      181 atcacttcag gagagattac atgaacaaaa atataaaata ttctcaaaac tttttaacga
      241 gtgaaaaagt actcaaccaa ataataaaac aattgaattt aaaagaaacc gataccgttt
      301 acgaaattgg aacaggtaaa gggcatttaa cgacgaaact ggctaaaata agtaaacagg
      361 taacgtctat tgaattagac agtcatctat tcaacttatc gtcagaaaaa ttaaaactga
      421 atactcgtgt cactttaatt caccaagata ttctacagtt tcaattccct aacaaacaga
      481 ggtataaaat tgttgggaat attccttacc atttaagcac acaaattatt aaaaaagtgg
      541 tttttgaaag ccgtgcgtct gacatctatc tgattgttga agaaggattc tacaagcgta
      601 ccttggatat tcaccgaaca ctagggttgc tcttgcacac tcaagtctcg attcagcaat
      661 tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa
      721 aacttacccg ccataccaca gatgttccag ataaatattg gaagctatat acgtactttg
      781 tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac taaaaatcag tttcatcaag
      841 caatgaaaca cgccaaagta aacaatttaa gtaccattac ttatgagcaa gtattgtcta
      901 tttttaatag ttatctatta tttaacggga ggaaataatt ctatgagtcg cttttttaaa
      961 tttggaaagt tacacgttac taaagggaat ggagataaat tattagatat actactgaca
     1021 gcttccaaga agctaaagag gtccctagcg cctacgggga atttgtatcg ataaggggta
     1081 caaattccca ctaagcgctc gggacccctt gtaggaaaat gtcctaagtg tggcaacaat
     1141 attgtatt
//
LOCUS       DAZKLA010000339         1003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3832
            SAMEA115706941-rid22582583.guided.281, whole genome shotgun
            sequence.
ACCESSION   DAZKLA010000339 DAZKLA010000000
VERSION     DAZKLA010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706941
            Sequence Read Archive: ERR13196675
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287835.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287835.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:32:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 37 of 91
            Pseudo Genes (incomplete)         :: 48 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706941-rid22582583.guided.281"
                     /isolate="VRE3832"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            287..835
                     /gene="aac(6')"
                     /locus_tag="ACG85N_003073"
     CDS             287..835
                     /gene="aac(6')"
                     /locus_tag="ACG85N_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0225733.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          353 a          145 c          209 g          296 t
ORIGIN      
        1 gattattata tgaaaacagg ttttatttgt caattcataa ttggtttttt ttaaagcaaa
       61 agataatcta tatcatgatg aacaaacaga gttttttcct ccaattgatg gctttcctga
      121 tctttcctat tttgaataaa gtgtttctgt ataatttttt tattttataa gtaagataaa
      181 taatgaaaga atagagatac tgattggtag ctaaagattt ttgagcgaga acatttcgtt
      241 ttttcaaaag gactgttaga ataagcaaga acaaggggga agacgtatga taatcagtga
      301 atttgaccgt aataatccag tattgaaaga tcagctttct gatttactga gactgacttg
      361 gccggaagaa tatggagaca gctcggcaga agaagtagaa gaaatgatga atccagaacg
      421 aatcgcggta gcagcggtag accaagatga gttagtagga tttattggtg caatccctca
      481 atacggtatc acaggttggg aattgcatcc attagttgta gaaagctccc gacgaaagaa
      541 ccaaataggt actcgattag tcaattactt agaaaaagaa gtagcttcca gaggaggaat
      601 cacgatttat ttaggtacgg atgatttaga ccatggaaca acgttaagtc aaacggacct
      661 gtatgagcat acatttgata aagtggcttc catccagaac cttcgtgaac atccgtatga
      721 attctatgaa aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa atggctggga
      781 caaaccggat atttggatgg caaaaacgat tattcctcga ccaaattctc aataataaaa
      841 agaggttgtg acagaagtaa tcagcttcaa gaaataagaa ggaatttacg aaaattactt
      901 cttgaatttt tgtgaaattc caacttattt ccgaagaagc tacttttgga acaccgtgta
      961 ccagaggttg tgaaaaagtc gcttagtttc gagtagtaag aaa
//
